LOCUS       DBNHQZ010000001        13790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_271051, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000001 DBNHQZ010000000
VERSION     DBNHQZ010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13790
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_271051"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1937
                     /locus_tag="AC16YU_00005"
     CDS             141..1937
                     /locus_tag="AC16YU_00005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HIR6063631.1"
                     /translation="MYRLPILLAVLLTACRAPSVVPAAAPDDRATHVRVNLIGFRAED
                     AKVAMVMSRGPVSGGFEVLDSATRRVVLSGVIVPVEAEPWAPFSHYYDADFSGLRTEG
                     RYVVRAGGAESPAFSVGAEAYRGAHEDLLAFMRQQRCGFNPFFDRMCHTRDGMSFYGP
                     MPDSTFIDASGGWHDAGDQLKYLITGSNATARMLLAFELSGAEFADAHDALGRSGANG
                     LPDVLDEAWWGLDWVRKLAAVPGALVHQVADDRDHRGWKLPYEDGVDYGWGPNAYRPA
                     YFATGRPQGLREFRSEATGVSNLAGRSAAALAMAHRVWPAGASDTLLSGDAMHLARAL
                     YAMGRADEGFQQGNSWGAPYRYNERTWADDMEWGAAELYRVTGEKAYLDDAVRYARLA
                     GTTSWMQHDSTGHYEFYPFVNVGHFALHPHVDSAFRDTLAAFYRDGIERSVARARTNP
                     WGVGVPFLWCSNNLVSALITQILLYQRMTGDTRYHEFMLRQRDWLLGRNPWGTSMFTG
                     LPREGERPVDVHTSTWALTRVDVPGGLVDGPIDAGTWGSLIGIALTAPDEFAAVQGDR
                     VVYHDDIGDYATNEPTMDGTAGAVFWMAWFGR"
     gene            complement(1984..2886)
                     /locus_tag="AC16YU_00010"
     CDS             complement(1984..2886)
                     /locus_tag="AC16YU_00010"
                     /inference="COORDINATES: protein motif:HMM:NF022588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HIR6063632.1"
                     /translation="MDMRNGCLAAAAGLVMWCGASDRAAAQIVASERGSVRQTVDGTV
                     ITVDYSRPSLRGRKDIFNTEVPRNMLWTPGADSATTISFSKDVTIDGTPVPAGRYSVW
                     MVTNRADWQVILDPDTEMFHVPHPPPDLDQVAFTVTPDTAAPFVETLTFSFPAVRSTG
                     ADLRMHWDRTAVDMAITVRPTRILTVTEAEASPYVGRYAVVARVDSAGDKTHAYETDL
                     RWSNDYLSGTLEFGSDWPVMDVYYAPAAEHVFNPVWVMNGTVAGVIDFLFFEFAMDES
                     GRAVSFELRGPEDELILTGSRTGP"
     gene            complement(2899..3300)
                     /locus_tag="AC16YU_00015"
     CDS             complement(2899..3300)
                     /locus_tag="AC16YU_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063633.1"
                     /translation="MKGEQTSRRAFLAMFAGLVAVPRHVRMPIDHPEPRPGIDASLVL
                     TREQLKGMPEKVLEVFDMIREIPEVADGLGCSCGCSVFPTYRSLLTCYYETGMARGCN
                     ICQGTARLAYKRHKEGQTLQQIRRAVDARFG"
     gene            complement(3302..3925)
                     /locus_tag="AC16YU_00020"
     CDS             complement(3302..3925)
                     /locus_tag="AC16YU_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063634.1"
                     /translation="MTRRILFAARNALPLLLVGTMIQCGPAASGARVNDAEAQIAAAV
                     KAAPTEFQEGASVLGFASDGQVVSIREGTNDLVCLADDPAREGWSVACYHASLEPFMT
                     MGRELRAQGITDGGELARLRWEAAEAGTLAMPEAPAALYVLNGEAFDPATGEITSPYL
                     RWVIYTPWATTESTGLASQPEFPGQPWLMFPGTAGAHIMVSPPQTEE"
     gene            4162..4791
                     /locus_tag="AC16YU_00025"
     CDS             4162..4791
                     /locus_tag="AC16YU_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR6063635.1"
                     /translation="MSSSVRAFAIWSVCLVALPANCVSPSGTTPVRASDRPLDEAPSN
                     DPEGRRLATVESDLLALVNRERGSASPSRRALRRDPGADRIILWHVAAMADGRFLSHS
                     DPRGRDTGDRVLAYAADRATRCSEIVQWWSGPPDGRAHYDGYFRSPTHHAAYMEEGDF
                     DLGPGTLVGVAAVSGPGPAGTAYEGVSGSYTGMLFCDRPVALSVDPFDD"
     gene            4895..5845
                     /locus_tag="AC16YU_00030"
     CDS             4895..5845
                     /locus_tag="AC16YU_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063636.1"
                     /translation="MHRLLLAGLLAVFSLPAVAQPALRAAGPPVALTRAGDGPFTGPL
                     WSPDGSLLAFSSAGYGGIWILNADGSGLRRLTDADGAGFEFSWSPDGSRILARVARDE
                     NRRRSHAVTVFRVADGSSRALTEFRPDMPSLPMWAPGTADVVLPTPAGLERLDAGLPA
                     AARRAGAAERFVALRDGRPVVGNASARTAEPLDRFADETLLNLVTSPDGSRVAFEVLG
                     GNAWVLDPASGRAIDLGRGYRPQWSPDGRWIVYMVTEDDGERYLSSELVAVRSDGSAR
                     VQLTDTPDRQEMNPSWSPDGTRLAYDDILDGVVYVLPIAE"
     gene            5854..8367
                     /locus_tag="AC16YU_00035"
     CDS             5854..8367
                     /locus_tag="AC16YU_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6063637.1"
                     /translation="MIRIRIALLAALLVAAAPASMAQPVSGLSGWTVFLDPGHSQTQN
                     VGIYGYSEAHKNLRVGLALRQLLLQTTDIDTVYISRTNDVQEVSLSQRVDHANATGAD
                     FFMSIHSNAAGPTANSALTLWPQHRDLSEVSPLGGKPMAEAVVDRLVEGMRIPRSGAI
                     GECTFYGVDQCRGVTTTGKGGSRNYVQSYTNMASELSEAGFHTNPTQNQRNMNADWKR
                     LEAQAIYWAILQYHGLARPQVHILAGIVSDLESGLPINGALVSVQDTTYVTDTWELLF
                     NTYSSDPDLLRNGFFYLDGLVDGAHTVTAAADGFMSYEDTVDMADTTFTFHDIGLISM
                     TPPTVVSTDPSDGQDPFRVSDPIVIDFSRPMDRASVEAALEIDPGIPGTITWSAGDRR
                     MTLRPDSLVERTLYTLRIVDTAFGAYLHPFDGDGDGLAGGDFEISFTTGFPDTQPAFI
                     TASHPRQNGKEVPLFPVLNLVYNEPLDSLSIDGKVRLLQGGTTEVDGTVRYYRYGERG
                     VITFFPAAELYAATPYTLEVAPGVSDLFLNAETSVKRLNFTTGRLVETVDPIDTFEGA
                     STGFWWQPQQSGSTMGIASLGTAMSADQAIANGLFGGSQAMRLDYAWDTEAGSWLIRE
                     YLGSGPAFDRVFDQGNALRVYVFGDGSGTQFRFAVDDRVPVAAAGNHEVSPWYTVDWI
                     GWQPVTWVLYEDEVGSWIGDGVLDGNLRIDSFQLSYVDGAASSGTLWFDDLQLVQAFA
                     TGAEDEGELPDDFTLGQNYPNPFNPTTTIEFSVPASAHVRLAVYDLLGRTVAVLRDGV
                     LPAGRHSATFDARDLASGVYLYRLETPGRVQSRTMLLLK"
     gene            8435..9586
                     /locus_tag="AC16YU_00040"
     CDS             8435..9586
                     /locus_tag="AC16YU_00040"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6063638.1"
                     /translation="MSPSRPASILLALALLGTSAACSQPIDQESTLVDYRLVTVASGL
                     SHPWSIAFLPGGDMLVTERPGRLRIIRDGVLIETPVPGVPEVFAQGQGGLLDVALHPE
                     FESNGLVYLSYSKPLADGSTTAVVRGRFEEDRLVDVEEIFEAQTRGRGHYGSRLAFDR
                     DGYLFVTVGDRQAPPEGDLSAHPAQDLSNHHGTINRLHDDGRIPEDNPFVGVEGALPE
                     IWSYGHRNPQGLAIHPETGVVWSDEHGPQGGDELNLVLPGRNYGWPVIGYGVNYGGAT
                     IHDSTAHEGMVQPVHYWVPSIATSGLLIYTGEPFREWKGNLFVGGLAGVQLARLTMDG
                     NTVVAEETLVQGVGRIRDVRQGPDGLIYLAIDSDGGPMSIVRLEPVRAE"
     gene            9649..11520
                     /locus_tag="AC16YU_00045"
     CDS             9649..11520
                     /locus_tag="AC16YU_00045"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HIR6063639.1"
                     /translation="MPASARFRVFRFLLTAAACAALLPGTARSQAAEKAGPVFADGQA
                     QVVAAFEDPDQWIREELWVETEFDSDGDGRKDRVHVSVARQRQTETEGLKVPVIYGSS
                     PYYAGTASGDEFFWDVHQEPGDPPPPRAAAEPPRLRLRPFISDGFIDTWLPRGFAVVH
                     SESPGTGLSEGCPTVGAPNESLAPKAVVDWLNGRARGFRTVDGQEQVRATWSTGKVGM
                     TGTSYNGTLPLAAATTGVEGLEAVIPDAPNTSYWHYYRANGLVRNPGGYPGEDIDVLY
                     DFIHSRDSERRAWCDANVRDGEMAAGQDRTTGDYNDFWRGRDYTHAVDGIRAAVFLSH
                     GLSDWNVMPDHSIRIYRTLREQGKTARLYLHQGGHGGPPPLEMMNRWFTRFLYGVENG
                     VEDEPRSWIVREDAERDAPTPYADYPHPDAEPVTLYPSSGGHGTGSLSPGHDYWAGAE
                     TIVDNVSFGAQTLATAEWTEHRLLFATPVLADSLHLSGTARVTIRASADRPAVNLSAY
                     LVSLPWSERRTWQGQVIRQGGLVTRGWADLQNHASLTESEPLVPGEFYSMSFDLEPDD
                     QVIPAGQRIGLMIFSSDRDFTLWPRPGAELTVDLDGTSLRLPVVGGAAAYEAATGGR"
     gene            11522..12547
                     /locus_tag="AC16YU_00050"
     CDS             11522..12547
                     /locus_tag="AC16YU_00050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Atu2307/SP_0267 family LLM class monooxygenase"
                     /protein_id="HIR6063640.1"
                     /translation="MEIGLYSFAEIAPDRQSGGATGAGERLRHLVEEIELADRVGLDV
                     FGVGEHHRPDYTVSAPAVVLAAAAARTSRIRLTSAVSVLSSDDPVRVYQQFSTLDLLS
                     EGRAEIMAGRGSFIESFPLFGYPLEDYDELFSEKLDLLLQLRDQDRVRWSGRHRAPID
                     GLGVYPRAEGPLPVWIAVGGSPPSVVRAARLGLPMALAIIGGAPARFVPLVRLYRETA
                     REAGIDPVPPVSINSHGWIADDPKRAADEAFPPFAATMTRIGRERGWPPTTRAQFDAE
                     CSPQGALLVGSPEQVIEKILFEHGLFGMDRFLVQLTVGPMPHDRVLRAIELLGTVVAP
                     AVRRALA"
     gene            complement(12554..13279)
                     /locus_tag="AC16YU_00055"
     CDS             complement(12554..13279)
                     /locus_tag="AC16YU_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063641.1"
                     /translation="MRAFTICFSVLLAALLGAGGCDSGGEVDLGLPGIPEPIDPADFV
                     DRVDNPYWPLAPSTTYSYAWEEDGEPVTDEVEVLSETRMVNGVEATVVRDRLYRNGAL
                     EEETWDWFAQDKDGNVWYLGEESYTITGAQRVLDQGSWESGVDGARAGLYMPAEPTVG
                     DYYYQEYRRGRAQDVAEVVALGRDVQVPAGVFKNCVEIRETTPLEPDALEIKSFCPAV
                     GLALTVDVAEGGTRQELVMWGGL"
     gene            complement(13404..>13790)
                     /locus_tag="AC16YU_00060"
     CDS             complement(13404..>13790)
                     /locus_tag="AC16YU_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063642.1"
                     /translation="VRGLDVFELMPSEHLTENEIAAATLARFSEFNAQSQPRYTWPTS
                     FIVANAYVDQLVRAGAMAADRADRLRGDFTRVQSGEGRAAGLRAMDAAADALDAEARA
                     EADAGHGHVVRSALSRQIRELAVAFR"
BASE COUNT         1993 a         4944 c         4740 g         2113 t
ORIGIN      
        1 acgagatcgc cgcggcgcgc tcgaggctgc ggctggccca cctgaaggcg catctcgaga
       61 cttcccgagt cctcacggac caccagctca tgctctatcg ccgcgcccgc ggctacggcg
      121 ggcatcactg acctgtctcc atgtaccggc ttccgatcct cctcgccgtc ctcctcaccg
      181 cgtgccgggc gccgtccgtc gttcccgccg cggcgccgga cgaccgggcc acccacgtcc
      241 gggtgaacct catcggcttc cgggctgagg acgccaaggt ggccatggtg atgtcccgcg
      301 gaccggtctc gggcggattc gaggtcctgg actccgccac gcgccgcgtg gtcctgtcgg
      361 gcgttatcgt cccggtcgag gccgaaccgt gggcgccctt ttcccactac tacgacgcgg
      421 atttctccgg cctgcgcacg gagggccggt acgtggttcg ggcgggcgga gccgaatcgc
      481 cggcgttctc agtcggggcg gaagcctacc gcggcgccca cgaggacctc ctggccttca
      541 tgcgccagca gcgctgcggc ttcaatccgt tcttcgaccg catgtgccac acccgcgacg
      601 ggatgagctt ctacggcccg atgccggact cgacgttcat cgacgcctcg ggtggatggc
      661 acgatgccgg cgaccagctc aagtacctga tcacgggcag caacgcgact gcccgcatgc
      721 tgctcgcgtt cgagctctct ggcgcggagt tcgccgacgc gcacgacgcg ctgggccggt
      781 ccggggccaa cgggcttccg gacgtactgg acgaagcgtg gtggggcctc gactgggtcc
      841 gcaagctggc ggccgtgccg ggggcgctcg tccaccaggt ggcggacgac cgggaccacc
      901 gcggatggaa gctcccgtac gaggacggcg tcgactacgg ctgggggccg aacgcctacc
      961 gaccggctta cttcgcgacg ggccggccgc agggcctgcg cgaattccgc agcgaggcga
     1021 cgggcgtctc caacctcgcg ggccggtccg cggccgccct cgcgatggcg caccgggtct
     1081 ggcctgcggg ggcgtccgac acgctgctct ccggcgatgc catgcacctg gcccgggccc
     1141 tgtatgccat ggggcgcgcc gacgagggct tccagcaggg caactcctgg ggagcgccct
     1201 accgctacaa cgagcggacg tgggccgacg acatggagtg gggcgcggcc gagctctacc
     1261 gggtcacggg cgagaaagcg tacctggacg acgcggtccg ctatgcgcga ctggccggca
     1321 cgacgtcgtg gatgcagcac gactcgaccg ggcactacga gttctacccg ttcgtgaacg
     1381 tgggccattt cgccctccat ccgcacgtgg actcggcctt ccgggatacg ctggccgcat
     1441 tctaccgcga cggcatagaa cggtccgtgg cccgtgcccg caccaatccc tggggagtcg
     1501 gggtgccgtt cctctggtgc tcgaacaacc tcgtctccgc gctcatcacg cagatcctgc
     1561 tgtaccagcg catgaccggg gacaccaggt accacgaatt catgctccgg cagcgcgact
     1621 ggctgcttgg gcgcaatccc tggggcacct ccatgttcac gggcctgccg cgggagggag
     1681 agcgccccgt ggacgtccat acgtccacct gggcgctcac gcgcgtcgat gttccgggcg
     1741 ggctcgtcga cggtccgatc gatgccggga cgtggggcag cctgatcggc atcgcgctca
     1801 cggccccgga cgagttcgcg gccgtgcagg gggaccgcgt ggtgtatcac gacgacatcg
     1861 gcgactacgc cacgaacgag ccgacgatgg acggcacggc gggggccgtg ttctggatgg
     1921 cgtggttcgg ccgctgagcc ggttcggcgc gccccggcgt ccgccgagat cctgcctccc
     1981 gggtcagggg cctgttcgcg acccggtcag gatgagctcg tcttccggtc cgcggagctc
     2041 gaacgaaacg gcgcggccgc tctcgtccat cgcgaactcg aagaagagga agtcgatcac
     2101 gcccgccacc gtgccgttca tgacccagac cgggttgaag acgtgctccg cggcgggggc
     2161 gtagtagaca tccatcaccg gccagtcgga cccgaattcg agggtccccg aaaggtagtc
     2221 gttcgaccac cgcaggtccg tttcgtacgc gtgggtcttg tcacccgccg aatccacgcg
     2281 cgcgacgacg gcgtagcgcc ccacgtacgg cgacgcctcc gcctcggtca ccgtcaggat
     2341 gcgtgtcggc cgtaccgtga tggccatgtc cacggctgtc cggtcccagt gcatccgcag
     2401 gtcggcgccg gtcgagcgga cggcgggaaa cgagaacgtc agggtttcca cgaacggcgc
     2461 ggccgtgtcc ggggtcacgg tgaaagcgac ctggtccagg tcgggcggcg gatgcgggac
     2521 gtggaacatc tcggtgtccg ggtccaggat cacctgccag tccgcgcgat tggtgaccat
     2581 ccagaccgaa taccggcctg ccggtaccgg cgtgccgtcg atcgtcacgt ccttcgagaa
     2641 cgagatcgtc gtggccgaat cggcgcccgg cgtccagagc atgttgcgcg ggacctcggt
     2701 gttgaagata tcctttcggc cgcggagcga cggtcgtgag tagtcgaccg tgatcaccgt
     2761 accgtcgacg gtctggcgga ccgagccccg ctcgctggcg acgatctggg ccgccgcccg
     2821 gtcgctcgcc ccgcaccaca tcaccaggcc cgccgccgcg gccagacaac cgtttcgcat
     2881 gtccatgtcc ttggaggctc agccgaagcg ggcgtcgacc gcccgccgga tctgttgcag
     2941 ggtctgtcct tccttgtgac gcttgtacgc cagcctggcc gttccctggc agatgttgca
     3001 gccccgcgcc atcccggtct cgtagtagca cgtgagcagc gaccggtacg tcggaaagac
     3061 ggagcatccg cacgagcagc cgaggccgtc ggccacttcg ggaatctcgc ggatcatgtc
     3121 gaagacctcg aggaccttct ccggcatccc cttgagctgc tcgcgggtca ggaccagcga
     3181 cgcgtcgatg cccggccgcg gttcgggatg gtcgatgggc atacggacgt gtcgcggaac
     3241 ggcgaccagg cccgcgaaca tcgccaggaa agcccggcgc gatgtctgct ctcctttcat
     3301 gtcattcctc ggtctggggc ggcgatacca tgatgtgggc accggccgtg cccggaaaca
     3361 tcagccacgg ctggccgggg aactccggct gcgaggccag tccggtggac tcggtcgtcg
     3421 cccacggcgt gtagatcacc cagcgcaggt acgggctggt gatctcgccg gttgcggggt
     3481 cgaacgcctc cccgttcagc acgtagagtg cggcaggcgc ttcgggcatg gccagcgtgc
     3541 cggcctcggc cgcttcccag cgaagccggg cgagctcgcc cccgtcggtt atcccctggg
     3601 cccgcagctc gcggcccatg gtcatgaacg gctcgagcga ggcatggtag cacgccacgc
     3661 tccagccctc gcgggcgggg tcgtccgcga ggcacacgag atcgttcgtg ccctcccgaa
     3721 tgctcacgac ctgcccgtcg gacgcgaaac cgaggacgga ggcgccctcc tggaactcgg
     3781 tgggcgccgc cttgacggcg gccgcgatct gtgcttcggc atcgttgacc cgcgcgccgg
     3841 acgcggccgg gccgcactgg atcatggttc cgacgagcag cagcggcagg gcgttccgcg
     3901 ctgcgaagag gatgcgacga gtcatggggg tgcgcggcgg gcgcgcgggc tgggtaccga
     3961 cggatccggt cggctcgttc ggagcggcca cgggcagggg gttcgccgga cgcgcttcgg
     4021 tgatagcgac atgaaggtag cacaaaccgg gtatgccgac caagggcaac gggatgcggc
     4081 attggaaccg tccggtttcg atccgcttta tgggcactcc ccttccggcc gatccttccc
     4141 ggacaccgtg atcggtatcc catgagcagt tcggtacgtg ccttcgccat ctggagcgtc
     4201 tgtctggttg ccctgccggc gaattgcgtc tccccatccg gcacgacgcc cgtccgggcc
     4261 tccgaccggc cgctcgacga ggcgccctcg aacgatccgg aggggcgccg cctggcgacc
     4321 gtggaatccg acctcctcgc cctggtcaac cgggaacgcg gatcggcttc gccgtcccgg
     4381 cgcgcgctcc gccgcgaccc gggagcggac cggatcatcc tgtggcacgt ggcggccatg
     4441 gccgacggac ggttcctgag ccacagcgac ccgcgagggc gggacaccgg agaccgcgtg
     4501 ctggcctacg ccgcggaccg cgccacgcgg tgctcggaga tcgtgcagtg gtggagcggg
     4561 ccgcccgacg gccgcgccca ctacgacggg tacttccgca gcccgaccca ccacgcggcg
     4621 tacatggagg agggcgactt cgatctcgga cccggcacgc tcgtcggggt cgccgcggtg
     4681 tcgggcccgg gcccggccgg aaccgcctac gagggggttt ccgggagcta cacgggcatg
     4741 ctgttctgcg accggccggt cgcgctctcg gtcgatccgt tcgacgactg agtggcctcg
     4801 gggccggggc ttgcggggtg attggggctt caggggaaac ccggccggcg cccgtcggtt
     4861 gaggccgtgc actcttccga ccacctcccc gccgatgcac cgtctccttc tcgccggcct
     4921 cctcgccgtc ttctccctcc cggccgtggc ccagcccgca ctccgcgccg ccgggccacc
     4981 cgtcgccctc acgcgcgccg gagacggacc cttcaccggc cccctgtggt ccccggacgg
     5041 gtcgctcctc gcgttcagct ccgcgggata cggtggaatc tggatcttga acgccgacgg
     5101 ctcgggtctg cgccgactga ccgacgcgga cggggccggc ttcgaattct cctggtcccc
     5161 ggacgggagc cgcatcctcg cgcgcgtcgc tcgggacgag aaccgccgac gcagccacgc
     5221 cgtcacggtg ttccgtgtgg cggacgggtc ctcgcgcgcc ctcacggaat tccggccgga
     5281 catgccgtcg ctcccgatgt gggcgcccgg caccgccgac gtggtcctcc ccaccccggc
     5341 cgggctggaa cgcctggacg cgggacttcc agccgccgcg aggagggcgg gagcggccga
     5401 gcgcttcgtc gcgctgaggg acggtcgacc ggtggtcggc aacgcttcgg cgcgcaccgc
     5461 cgagccgctg gatcggttcg cggacgagac cctgctgaac ctggtcacgt cacccgacgg
     5521 aagccgcgtc gcgttcgagg tcctgggggg caacgcgtgg gtgctggacc cggcgtcagg
     5581 acgcgcgatc gacctcggcc gcggctaccg gccgcagtgg tcgcccgacg gtcgttggat
     5641 cgtctacatg gtgaccgagg acgacggcga acggtacctc tcgtccgagc tcgtcgccgt
     5701 ccggtccgac ggcagcgccc gggtgcagct gaccgacacg ccggaccgcc aggagatgaa
     5761 tccttcctgg tcgcccgacg ggacccgcct cgcctacgac gacatcctcg acggtgtcgt
     5821 gtacgtcctc cccatcgccg aatgaagccc cccatgatcc gcattcgcat cgcgctcctc
     5881 gcggccctcc tcgtcgccgc cgcgcccgcg tccatggcgc agcccgtctc cggcctttcc
     5941 gggtggaccg ttttcctgga ccccgggcac agccagaccc agaacgtcgg catctacgga
     6001 tactcggagg cgcacaagaa cctgcgcgtg ggtcttgccc tgcgccagct gctgctccag
     6061 acgacggaca tcgacaccgt ctacatctcc cgcacgaacg acgtccagga ggtctccctg
     6121 tcccagcgtg tcgaccatgc caacgccacg ggagcggact tcttcatgtc gatccactcg
     6181 aacgcggccg ggcccacggc caactccgcg ctgacgctgt ggccgcagca ccgtgacctg
     6241 agcgaggtct cgccgctcgg cggcaagccc atggccgagg ccgtggtgga ccgactcgtt
     6301 gagggcatgc ggatcccgag gtcgggcgcc atcggcgagt gcacgtttta cggcgtggac
     6361 cagtgccggg gcgtgacgac gaccggcaag ggcggatccc gcaactacgt gcagagctac
     6421 acgaacatgg cgtcggagct ctccgaggcc ggtttccaca cgaacccgac acagaaccag
     6481 cgcaacatga acgccgactg gaagcggctc gaggcgcagg cgatctactg ggcgatcctc
     6541 cagtaccacg ggctcgcgcg cccgcaggtg cacatcctgg cgggcatcgt gtccgacctg
     6601 gagtcgggcc tgccgatcaa cggggcgctg gtctccgtgc aggacacgac ctacgtgacc
     6661 gacacctggg agctcctgtt caacacgtat tcgagcgacc cggacctgct gcgcaacgga
     6721 tttttctacc tggacggact cgtcgacggc gcgcacacgg tcacggccgc cgccgacggc
     6781 ttcatgtcgt acgaggacac cgtcgacatg gccgacacga ccttcacctt ccacgacatc
     6841 ggcctcatct cgatgacgcc gccgacggtc gtgtccaccg acccgtccga cggccaggac
     6901 cccttccggg tcagcgatcc gatcgtcatc gatttcagcc gcccgatgga ccgtgcgagc
     6961 gtcgaggcgg ccctcgagat cgaccccggg atcccgggga cgatcacctg gtcggcgggc
     7021 gaccgccgca tgaccctgcg gcccgactcg ctcgtagagc gcacgctcta cacgctccgg
     7081 atcgtggaca cggccttcgg cgcgtacctg caccccttcg acggggacgg cgacgggctg
     7141 gccgggggcg atttcgagat ctccttcacg accggcttcc cggacactca gccagccttc
     7201 atcacggcga gccatccccg ccagaacgga aaggaggtcc ccctcttccc cgtcctgaac
     7261 ctcgtctaca acgagcccct ggacagcctg agcatcgacg ggaaggtgag gctcctgcag
     7321 gggggcacca ccgaggtgga cggcacggtg cgctactacc gctacgggga gcggggggtc
     7381 atcacgttct tcccggcggc cgagctctac gccgcgaccc cgtacacgct cgaggtggct
     7441 cccggagtga gcgacctgtt cctgaacgcc gagaccagcg tgaagcgcct gaacttcacg
     7501 accgggcgcc tcgtcgagac ggtggacccg atcgacacgt tcgagggtgc ttcgaccggg
     7561 ttctggtggc agccgcagca gagcggctcc acgatgggga tagcgagcct ggggaccgcc
     7621 atgtcggccg accaggcgat cgcgaacggc ctcttcgggg gcagccaggc catgcggctg
     7681 gactacgcct gggacacgga ggccggatcc tggctgatcc gcgagtacct cgggagcggc
     7741 ccggccttcg acagggtgtt cgaccagggc aatgccttgc gcgtgtacgt gttcggagac
     7801 ggcagcggca cgcagttccg cttcgcggtc gatgaccggg tccccgtcgc tgccgcgggc
     7861 aatcacgagg tgagcccgtg gtacacggtc gactggatcg ggtggcagcc cgtgacgtgg
     7921 gtgctgtacg aggacgaggt cggctcctgg atcggtgacg gagtgctcga cggcaacctc
     7981 cgcatcgact cgttccagct ctcgtacgta gacggcgccg cctcgagcgg caccctctgg
     8041 ttcgacgacc tgcagctcgt gcaggcgttc gcgaccggtg cggaagacga gggcgagctg
     8101 cccgatgact tcacgctcgg gcagaactac ccgaacccgt tcaaccccac gacgaccatc
     8161 gaattctcgg tgccggcgtc cgcccacgtg cgcctggccg tgtacgacct gctcggccgg
     8221 accgtggccg tgctccggga cggcgtcctg ccggcgggcc ggcactcggc gaccttcgac
     8281 gcgcgcgatc tcgcgagcgg cgtgtacctg taccggctcg agacgccggg ccgcgtgcag
     8341 tcgcgcacca tgctgctgct caagtgagcc ggggcgcgcg aaacaccggg cggcccgcgc
     8401 cggttagcct gggacacgaa gacttcccgc acccatgagc ccgtcccgtc ccgcatccat
     8461 cctcctggcg ctcgcactcc tcggcacctc cgcggcctgc tcgcagccga tcgatcagga
     8521 atcgaccctc gtcgactacc gcctggtgac ggttgcctcg ggcctctcgc atccctggtc
     8581 gatcgccttc ctgcccggcg gggacatgct cgtcaccgag cggccggggc ggctgcgcat
     8641 catccgcgac ggcgtgctca tcgagacccc ggtgcccggc gtgccggagg tcttcgcgca
     8701 gggacagggc ggcctcctgg acgtggcgct ccacccggag ttcgagtcca acggcctggt
     8761 gtatctctcc tattcgaagc ccctcgcgga cggttcgacg acggccgtgg tgcgcggacg
     8821 gttcgaggag gaccggctcg tggacgtcga ggagatcttc gaggcgcaga cccgcgggcg
     8881 cggccattac ggctcccgcc tggcgttcga ccgggacggc tacctgttcg tgaccgtggg
     8941 cgaccggcag gcgcctcccg agggcgacct ttcggcgcat cccgcccagg acctgtccaa
     9001 ccaccacggg acgatcaacc gtctgcacga cgacgggcgc attcccgagg acaacccgtt
     9061 cgtgggcgtg gaaggcgcgc tgcccgaaat ctggagctac gggcaccgca acccgcaggg
     9121 gctcgcgatc catccggaga cgggcgtcgt ctggtcggac gagcacgggc cgcagggggg
     9181 tgacgagctg aacctggtcc ttcccggacg caactacgga tggcccgtga tcggctacgg
     9241 ggtgaactac ggtggcgcca cgatccacga ttcgaccgcg cacgagggca tggttcagcc
     9301 ggtccactac tgggtgccgt cgatcgccac gagcggcctg ctcatctaca cgggcgagcc
     9361 gttccgcgag tggaagggca acctgttcgt cgggggcctg gccggggtgc agctcgcccg
     9421 gctgaccatg gacggcaaca cggtcgtggc cgaggagacc ctcgtgcagg gtgtgggacg
     9481 cattcgggac gtgcgccagg gtccggacgg gctcatctac ctggccatcg actcggacgg
     9541 cggaccgatg tccatcgtgc gactggaacc ggtccgggcg gagtgaccct gcgccgatcg
     9601 gcgccggacg ctacctttcg ccttccacag aacatcgagg gtgaacccat gccggcgtcc
     9661 gcgcgcttcc gagtcttccg tttcctgctg accgccgcgg cgtgcgcggc cctcctgccc
     9721 ggcaccgccc gctcccaggc tgccgagaag gccggccccg tgttcgcgga cggacaggcc
     9781 caggtcgtcg cggcgttcga ggatccagac cagtggatcc gcgaagagct ctgggtggag
     9841 acggagttcg actcggacgg ggacggacgg aaggaccgcg tgcacgtctc ggtcgcgcgc
     9901 cagcggcaga ccgagaccga ggggctcaag gtcccggtca tctacggctc gagcccgtat
     9961 tacgcgggca ccgcgagcgg ggacgagttc ttctgggatg tgcaccagga gcccggcgat
    10021 cctcccccgc cccgcgccgc cgccgagccc ccgcgcctgc gcctgcgccc gttcatctcg
    10081 gacggcttca tcgacacgtg gctgccccgc ggcttcgcgg tcgtgcactc ggagtcgccg
    10141 ggaacggggc tctccgaggg atgtccgacg gtcggcgctc ctaacgaatc gctcgccccc
    10201 aaggccgtcg tcgactggct gaacggccgg gcgcgcggct tccgcacggt ggacggccag
    10261 gagcaggtcc gcgcgacctg gtcgaccggc aaggtgggca tgacgggcac gtcgtacaac
    10321 ggcacgctgc cgctcgccgc cgcaacgacc ggcgtagagg gcctggaggc cgtgattccg
    10381 gacgcgccca acacctcgta ctggcactac taccgggcga acgggctcgt gcgcaacccc
    10441 ggcggctatc cgggcgagga catcgacgtg ctctacgact tcatccacag ccgggactcc
    10501 gaacgccggg cctggtgcga cgcgaacgtc cgggacggcg agatggccgc gggccaggac
    10561 cggacgacgg gcgactacaa cgacttctgg cgcggccgcg actacaccca cgctgtggac
    10621 ggcatccggg cggcggtttt cctttcccac gggctgagcg actggaacgt gatgcccgac
    10681 cacagcatcc ggatctaccg gacgctccgg gagcagggca agaccgcgcg cctgtacctg
    10741 caccagggcg ggcacggtgg accgccgccg ctcgagatga tgaaccggtg gttcacgcga
    10801 ttcctgtacg gcgtggagaa cggtgtcgag gacgagccgc gctcctggat cgtccgcgag
    10861 gacgccgagc gcgacgcgcc gaccccgtac gccgactacc cgcaccccga cgcggagccg
    10921 gtgacgctct atccgtcctc cggcgggcac ggcaccggct cgctgagccc cgggcacgac
    10981 tactgggcgg gagcggagac gatcgtcgac aacgtttcgt tcggggccca gaccctggcc
    11041 acggccgagt ggacggagca ccggctgctc ttcgccaccc cggtcctcgc cgactcgctg
    11101 cacctgtccg gcacggcccg tgtcacgatc cgggcctccg cggaccgccc ggcggtcaac
    11161 ctgtcggcgt acctggtttc gctgccctgg agtgagcgcc gcacctggca gggccaggtc
    11221 atccggcagg gcggcctggt cacgcgaggc tgggccgatc tccagaacca cgcgtcgctc
    11281 accgagagcg agcccctcgt tccgggcgag ttctactcga tgtccttcga cctggagccg
    11341 gacgaccagg tgatcccggc cggccagcgc atcgggctca tgatcttctc gagcgaccgg
    11401 gacttcacgc tgtggccgcg cccgggcgcc gagctcacgg tggacctgga cgggacctcg
    11461 ctgcggctgc cggtcgtcgg gggcgccgcg gcgtacgagg cggcgaccgg cggccggtga
    11521 catggagatc gggctgtatt ccttcgcgga gatcgcgccg gaccggcagt cgggcggcgc
    11581 gaccggcgcc ggggagcgcc ttcgccacct ggtcgaggag atcgagctcg ccgaccgggt
    11641 ggggctcgac gtcttcggcg tgggcgagca ccatcggccc gattacaccg tgtccgcgcc
    11701 cgccgtcgtc ctggcggcgg ccgcggccag gaccagccgg atccgcctca cgagcgccgt
    11761 ctcggtcctg agctccgacg accccgtccg ggtgtaccag cagttctcca ccctggacct
    11821 cctttcggag ggccgggcgg agatcatggc ggggcggggc tccttcatcg agtcgtttcc
    11881 gctcttcggc tatccgctgg aggactacga cgagctcttc tcggagaaac tcgacctgct
    11941 gctccagctt cgggaccagg accgggttcg ctggtccggc aggcaccggg ccccgatcga
    12001 cggcctgggc gtgtatccgc gagcggaggg gccgcttccg gtctggatcg cggtcggcgg
    12061 aagccctccg tcggtcgtcc gggcggcgcg cctggggctt cccatggcgc tcgccatcat
    12121 cggcggggcg ccggcccggt tcgtgcccct ggttcgcctg taccgcgaga cggcccggga
    12181 ggcgggcatc gacccggtgc ccccggtcag catcaactcg cacgggtgga tcgcggacga
    12241 tccgaaacgg gccgccgacg aggccttccc gccgttcgcg gcgaccatga cgcgcatcgg
    12301 gcgcgagcgg ggctggcctc cgaccacgcg cgcgcagttc gacgcggagt gctccccgca
    12361 gggcgccctc ctcgtcggca gcccggagca ggtgatcgag aagatcctct tcgagcacgg
    12421 gctgttcggc atggaccgct tcctggtcca gctcacggtc ggccccatgc cgcacgaccg
    12481 ggtcctccgg gccatcgagc tcctgggcac ggtcgtcgct ccggccgtgc gtcgggccct
    12541 cgcgtgagcg ggatcagagc ccgccccaca tgacgagctc ctggcgcgtg cccccctcgg
    12601 ccacgtccac cgtcagcgcc aggcccacgg ccgggcagaa gctcttgatc tccagcgcgt
    12661 cgggttcgag aggagtcgtc tcgcggatct ccacgcagtt cttgaacacg cccgccggca
    12721 cctgcacgtc ccgccccagc gcgaccacct ccgcgacgtc ctgggcccgg ccgcggcggt
    12781 actcctggta gtagtagtcc cccacggtgg gttcggcggg catgtagaga cccgcccggg
    12841 cgccgtccac tccggattcc cacgacccct ggtccaggac gcgctgggcc cccgtgatcg
    12901 tgtacgactc ctcgcccagg taccagacgt ttccgtcctt gtcctgcgcg aaccagtccc
    12961 aggtctcctc ctcgagcgca ccgttcctgt agaggcggtc ccggacgacg gtagcctcga
    13021 cgccgttcac catcctggtc tcggagagta cctcgacctc gtcggtgacc ggttcgccat
    13081 cctcctccca cgcatagctg tacgtcgtgc tgggggcgag tggccagtac gggttgtcca
    13141 cccggtcgac gaaatcggcc gggtcgatgg gttcgggaat tcccggcagg cccaggtcca
    13201 cctccccccc ggaatcgcac ccgcccgctc cgagaagcgc ggcgaggagc actgagaagc
    13261 atatggtgaa ggctcgcatg gtggtctcgc gtttggtcca tcggcggacc gccgcggccc
    13321 gccccgcggt cagtctaacg gctccagcgg tcgggggcga tacgggcaat cctttttccg
    13381 ttccgggccg agcagcgggg agatcatcgg aaggcgacgg ccagctccct gatctgacgc
    13441 gacagggccg agcggaccac gtgcccgtgc ccggcgtccg cctcggcccg cgcctcggcg
    13501 tcgagcgcat ccgcggcggc gtccatggcc cgaaggcctg ccgcgcgccc ttccccgctc
    13561 tgcaccctcg tgaaatcacc ccgcagccgg tcggcccggt cggccgccat cgctccggcg
    13621 cgcaccagct ggtccacgta cgcgttcgcg acgatgaaac tcgtcggcca ggtgtagcgg
    13681 ggctggctct gggcgttgaa ctcggagaag cgggcgagcg tggcggccgc gatctcgttc
    13741 tcggtcagat gctcgctcgg catgagctcg aacacgtcga gcccgcgcac
//
LOCUS       DBNHQZ010000002         6041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_813186, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000002 DBNHQZ010000000
VERSION     DBNHQZ010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_813186"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="AC16YU_00065"
     CDS             <1..818
                     /locus_tag="AC16YU_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063643.1"
                     /translation="GGIDRIAMGLGYRISEAIAVGVRADFLFGIIEDVRRVRYFDSRY
                     EGARLSEATRLRGLGLGLGVRAKTSSLFRDGDVASIGFSLDFPAHLTGERVVTAGATV
                     DPDTLVSGVDGSMDLPVGAGFGLAYQAGPRFVAIADLRYERWSGFESTFALPAYPSGG
                     QLDDRLRVSGGIEFYPGGRDLLAGYFRRTAYRLGAFYEQSYASPVPDTRINAYGLTGG
                     FSFPTLIPGTRVDVHAEVGNRGTAENMLIQDRYLRIGVSLNFADRWFVRRRLG"
     gene            871..2196
                     /locus_tag="AC16YU_00070"
     CDS             871..2196
                     /locus_tag="AC16YU_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6063644.1"
                     /translation="MKHRRLLPAAARQVLVLSLVMLGMGLRSVTAKAQACSEDQNEIA
                     MNYSLYYEDFKNGNFETALPYLRWILRCAPTFPSGRDTNFRRAVEVYEGIAAKAASPE
                     DQRVYLDSALYVFDTAMTNLQDAGIEPDSFYWYFEKGRFIQNHATELPDVQGEVGPIY
                     GTAFELDPARLQTYYINYIIADFVQKNDKGSAVDFMDQVESHRAGDQEVTDLLAQWRG
                     QLFTSPEERIGFLESQLEKNPEDADIMAELFELLMDEGHRDRVYELAPAMMATDPSAR
                     TYRLIGKMRLEDGQTEEAIRLYEESLSLPGGPEGARESYYNIGIAHQQEGRLSRARTA
                     FRQSLDADPAYGASLIAIGDLYVSAVQGCGTFEREDRAVYWLAADYYERAAARGSAPE
                     AAQARSRLAQIQRYMPTAEDKFFKGWTAGDSYTIDYGCYTWIGESTRIR"
     gene            2404..3693
                     /gene="eno"
                     /locus_tag="AC16YU_00075"
     CDS             2404..3693
                     /gene="eno"
                     /locus_tag="AC16YU_00075"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6063645.1"
                     /translation="MARIESIHARQILDSRGNPTIEVEVAAEDGSFGRAAVPSGASTG
                     EHEAVELRDGDKDRFLGKGVLDAVRNVNEVIAGELIGFNVLDQAEVDRVLIALDGTPN
                     KGRLGANAILGVSLATAYAGADTVGLPLYRYIGGTGARTLPVPQMNILNGGRHADNSV
                     DMQEFMIMPVGAATFSDGLRMGVEIFHNLKKVLAARGYSTAVGDEGGFAPNLGSNEEA
                     IEVILEAIGKAGYHAGDDVFIALDPAASEMVQDGKYVFWKSDPDHPKSSDEMVDFWAS
                     WVENYPIISLEDAMGENDWDGWRKLTETVGSRVQLVGDDIFVTNPEILRRGIEAGCAN
                     SILVKLNQIGTLTETLDAIELAHKHGYTTVISHRSGETEDTTIADLAVAVNSGQIKTG
                     SASRSDRLAKYNQLLRIEELLEHTAVYPGRRAFRFGR"
     gene            3708..4016
                     /locus_tag="AC16YU_00080"
     CDS             3708..4016
                     /locus_tag="AC16YU_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR6063646.1"
                     /translation="MRLVNRLRALSKRSRRRLVLAVVAAAVIFLGFLDSHSVYKRIVL
                     HREARRLEAENRVLQDEIDRLQAELDRELSDEEIERIAREQYGMRREGETVYPVKRDD
                     "
     gene            4009..5025
                     /locus_tag="AC16YU_00085"
     CDS             4009..5025
                     /locus_tag="AC16YU_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6063647.1"
                     /translation="MTDGATSNAADLLAVGVDLGGTSIKAALVSRSEGLVRQETHETE
                     AHLGPERVVDNIARLVQELTRGLGGGIAGIGIGAPGAIDLARTTVSYPPNFPGWERLH
                     MGEALGPRLGPLAGQVIVENDANVAGLGSAHYGAGLDYDSFIMVTLGTGVGGAIIYRN
                     RIFRGTTGAAGEIGHMTIDYEGPFARSGVAGAIEAYLGQRFLSRHARYRLMNRRESSI
                     HQMADYDLHGITPLMLYEAAVEGDEGAREMLAWAGHKLGCVLGSAINLLDIRKVVVGG
                     GLSAAGDYILEEARRTVRHFVTPALHEGIEIIRETRGNEVGMLGAAHLVFQSLDDIKE
                     DPHA"
     gene            5129..>6041
                     /locus_tag="AC16YU_00090"
     CDS             5129..>6041
                     /locus_tag="AC16YU_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR6063648.1"
                     /translation="MDRPVRFSATDSLVVSLGTDEGDLGRLYGGSTVDYGESKLEAWR
                     VEILFDLDELRADGLPSDTGLVGGPVFRQGDETFRGERLAFNLRTERGRVQAARTEIE
                     EGFIRADVVKAREDSTLFIRNGVYTTCPCVDDPSYSLRSSRMKIVNRKWIYTGPIQLY
                     LYGIATPLWLPFGFLPATEGRRSGPLPPSYGEDEFGFYLRDFGWYFALSDYMDLQLQG
                     SLWTRGSVEIRALSRYRKRYGYDGQVQISLGRFRSGERGDPDFSIRRTGSLRWTHGQP
                     ISPTANFDANVNLSSSGYLRGVSQDYDD"
BASE COUNT         1038 a         2042 c         1989 g          972 t
ORIGIN      
        1 acgggggcat cgaccgcatc gccatgggcc tgggataccg gatctcggag gcgatagccg
       61 tcggcgtccg ggccgatttc ctcttcggca tcatcgagga cgtccggcgc gtccgctact
      121 tcgattcccg gtacgagggg gcccgcctct cggaggcgac caggctccgc gggctcggcc
      181 tcgggctcgg ggtccgcgcg aagacgtcct ccctgttccg cgacggggac gtggcgtcca
      241 tcggcttctc cctggatttt ccggctcacc tgaccggaga gcgcgtcgta acggccggtg
      301 cgacggtcga tcccgacacg ctcgtttcgg gtgtcgacgg ctcgatggac ctaccggtgg
      361 gcgccggctt cggcctcgcc taccaggcgg gaccccggtt cgtggccatc gcagatcttc
      421 gatacgagcg ctggtccggg ttcgagagca cgttcgccct cccggcctac ccgtcgggcg
      481 gccagctcga cgaccggctg cgggtctccg gcggaattga attctatccg ggcggacgcg
      541 atcttctcgc cggttatttc cgtcgcacgg cctatcggct cggagcgttc tatgagcagt
      601 cgtacgcgtc ccccgtgccg gacacgcgga tcaacgcgta cggcctcacc ggtggattca
      661 gttttcccac gctgattccc ggcacgcgtg ttgatgtaca tgccgaagta ggaaaccgtg
      721 gaacggccga aaacatgctg atacaggatc gataccttcg catcggtgtc agcctgaatt
      781 ttgccgaccg ctggttcgtt cgacgccgtc tgggctgacg gtccccggtt tcccgaaatc
      841 ggaaatgcaa cggcaagacg caacagaacg atgaaacacc gcagactcct tcccgccgcc
      901 gcgcgccagg tcctggttct atcgctcgtg atgctcggaa tgggcctcag gagcgtcacg
      961 gcgaaggccc aggcctgctc ggaggatcag aacgagattg ccatgaacta cagcctctac
     1021 tacgaggact tcaagaatgg caatttcgag acggcgctgc cctacctgcg ctggattctg
     1081 cggtgcgccc cgacgtttcc gtcgggcagg gataccaatt tccggcgcgc ggtggaggta
     1141 tacgagggca tcgccgcgaa ggcggcttcc cccgaggacc agagggtgta cctggactcg
     1201 gcactgtacg tattcgacac ggcgatgacc aatttgcagg acgccgggat cgagcccgac
     1261 tccttctact ggtatttcga gaagggccgg ttcatccaga accacgcgac cgagctcccg
     1321 gacgtccagg gcgaggtcgg gccgatctac gggacggcct tcgaacttga ccccgcccgg
     1381 ctgcagacct actacatcaa ctacatcatc gcggacttcg tccagaagaa cgacaagggc
     1441 tcggccgtgg acttcatgga ccaggtcgag agccaccgtg cgggagacca ggaggtcacc
     1501 gatctgctcg cgcagtggcg cggccagttg ttcacttcgc ccgaggagcg catcggattc
     1561 ctggagagcc agctcgagaa gaatcccgag gatgccgata tcatggccga gctcttcgag
     1621 ctgctgatgg acgagggcca tcgtgaccgg gtctacgagc tggctcccgc catgatggcg
     1681 accgatccgt cggcgaggac ctaccgcctg atcggcaaga tgcggctcga ggacggccag
     1741 accgaggagg ccatccggct gtacgaggag tctctcagcc tgccgggcgg ccccgaaggt
     1801 gcacgcgaat cctactacaa catcggaatc gcgcatcagc aggagggtcg cctctcccgc
     1861 gcccggaccg cgttccggca gtcgctcgat gccgacccgg cgtacggcgc gtcgctgatc
     1921 gcgatcggcg acctgtacgt atcggcggtc cagggctgcg gcacctttga gcgcgaggac
     1981 cgcgccgtct actggctcgc cgccgactac tacgagcggg ccgccgcccg gggcagcgcg
     2041 cccgaggccg cccaggcccg gagccgtctc gcccagatcc agcggtacat gccgaccgcc
     2101 gaggacaagt tcttcaaggg atggaccgcc ggcgattcgt acacgatcga ctacgggtgc
     2161 tatacctgga tcggcgaatc gaccaggatc cgctgacctc gtccggcatc ccggagacca
     2221 gggccggtcc cccgcgggat cggccctttc ttcattcgtg tgcatcctgc gcgaatccgg
     2281 cagggcaggc cccggtgacg ccactcgttc cttgttattg ggaccttcga tcgcgaattt
     2341 cgtccggttg acgcggccag tccgcgcccc tcgattccac ctccgatccg cctgcatatc
     2401 ctcatggccc gcatagaatc catccacgca cgacagatcc ttgacagccg gggcaatccc
     2461 accatcgagg tcgaggtcgc ggcggaggac ggctcgttcg gccgcgccgc cgttccaagc
     2521 ggtgcttcca ccggtgaaca cgaggccgtg gagctccggg acggggacaa ggaccggttc
     2581 ctgggcaagg gggtcctgga tgccgtccgc aacgtgaacg aagtcatcgc gggcgagctc
     2641 atcgggttca acgtgctgga ccaggccgag gtggaccggg tcctcatcgc gctcgacggc
     2701 acgccgaaca agggacggct cggcgcgaac gccatcctgg gcgtgtcgct ggcgaccgcg
     2761 tatgccggcg ccgacacggt gggccttccc ctctaccggt acatcggcgg cacgggcgcg
     2821 cgtacgctgc ccgtacccca gatgaacatc ctgaacggcg ggcgccacgc agacaacagc
     2881 gtggacatgc aggagttcat gatcatgccc gtcggggcgg ccacgttctc ggacggcctg
     2941 cggatgggcg tggagatctt ccacaacctc aagaaggtgc tcgccgcgcg cgggtactcg
     3001 accgcggtcg gagacgaggg cggctttgcc ccgaacctcg ggtccaacga ggaagcgatc
     3061 gaggtcatcc tcgaggcgat cggcaaggcc ggctaccacg cgggcgacga cgtcttcatc
     3121 gccctggacc ccgccgcgag cgagatggtc caggacggca agtacgtgtt ctggaagagc
     3181 gatcccgacc acccgaaatc gtcggacgag atggtcgatt tctgggcttc atgggtcgag
     3241 aactacccga tcatctcgct cgaggacgcg atgggcgaga acgactggga cggctggcgg
     3301 aagctgaccg agaccgtggg atcgcgcgtt cagctcgtgg gcgacgacat cttcgtcacc
     3361 aatccggaga tcctgcgcag gggcatcgaa gcagggtgcg cgaactccat cctggtcaag
     3421 ctcaaccaga tcggcacgct gaccgagacc ctcgacgcga tcgagctcgc ccacaagcac
     3481 ggatacacga cggtcatcag ccaccgctcg ggcgagaccg aggacaccac gatcgccgac
     3541 ctggccgtcg ccgtgaactc gggccagatc aagaccggct ccgcgagccg cagcgaccgc
     3601 ctggccaagt ataaccagct gctccgcatc gaggagctcc tggagcatac ggccgtgtac
     3661 ccgggccgcc gcgctttccg cttcggacgc tgagcccttc ggacgtcatg cgcctcgtca
     3721 accgcctgag ggccctctcc aagcgttcac gtcgacgcct cgtcctggcc gtcgtcgcgg
     3781 cggccgtgat cttcctgggc ttcctggaca gccacagcgt gtacaagcgg atcgtgctgc
     3841 atcgcgaggc ccgtcgcctg gaagccgaga accgcgtgct ccaggacgag atcgaccgcc
     3901 tgcaggcgga actggaccgc gagctgagcg acgaggaaat cgaacggatc gcccgggagc
     3961 agtacggcat gcgccgcgag ggcgagaccg tgtatccggt caagcgcgat gactgacggc
     4021 gcgacttcga acgcggcgga cctcctggcc gtgggcgtgg acctcggggg caccagcatc
     4081 aaggccgccc tcgtctcccg gtccgagggc ctcgtgcggc aggaaaccca cgagaccgag
     4141 gcacacctgg gcccggaacg ggtcgtggac aacatcgcgc ggctcgtcca ggaactcacg
     4201 cgggggctcg gagggggcat cgcgggcatc gggatcggcg cacccggggc gatcgacctg
     4261 gcccggacca cggtctccta ccctcccaac ttccccggct gggaacgcct tcacatgggc
     4321 gaagccctcg gtccgcggct gggccccctg gccggtcagg tcatcgtcga gaacgacgcg
     4381 aacgtggcgg gactcggctc cgcccactac ggcgcgggcc tggattacga ttcgttcatc
     4441 atggtcacgc tcgggacggg cgtgggcggc gcgatcatct accggaaccg cattttccgc
     4501 ggaacgaccg gcgccgcggg cgagatcggc cacatgacca tcgactacga gggaccgttc
     4561 gcccgctccg gcgtggccgg ggcgatcgag gcctacctcg gacagcgatt cctttcccgg
     4621 cacgcgcgct accggctgat gaaccgcagg gagtcgtcca tccaccagat ggcggactac
     4681 gacctgcacg gcatcacacc gctcatgctc tacgaggcgg ccgtcgaggg cgacgagggc
     4741 gcgcgcgaga tgctggcctg ggcgggccac aagctcggat gcgtcctcgg ctcggccatc
     4801 aacctgctgg acatccggaa agtcgtggtc gggggcggtc tctcggccgc cggagactat
     4861 atcctggagg aggcgcggcg cacggtgcgg catttcgtga cgcccgcgct ccacgagggc
     4921 atcgagatca tccgcgaaac ccgcggcaac gaggtcggta tgctcggggc ggcgcacctc
     4981 gtattccagt ctctcgatga catcaaggag gacccgcacg cctgaccgcc gccgatccgt
     5041 ctcccggatc ggagcccttc ggggtgcggc gtgcggggtg ctctgcctgt tgtggaccct
     5101 gcccgccgcc gcgcagacga gcggcagcgt ggaccgtccg gtccgcttca gcgcgaccga
     5161 ttcgctcgtc gtctcgctgg gcaccgacga gggcgacctc ggacgcctct atggaggctc
     5221 gacggtggac tacggcgagt cgaagctgga ggcctggcgc gtggagatcc tcttcgacct
     5281 cgacgaactc cgcgcagacg gcctcccatc ggacaccggt ctcgtgggcg gtcccgtctt
     5341 ccggcaggga gacgaaacgt tccggggaga gcggctcgcc ttcaacctgc gcaccgagcg
     5401 gggacgcgta caggcggcgc gaaccgaaat cgaggaaggg ttcatccggg ccgacgtcgt
     5461 caaggcccgc gaggacagca ccctgttcat ccggaacgga gtatacacga cgtgcccctg
     5521 cgtcgacgat ccgtcgtact cgctccgatc gtcgcgaatg aagatcgtga accggaaatg
     5581 gatctatacc gggccgatcc agctgtacct gtacggcatc gcgacgcccc tgtggctccc
     5641 cttcggcttc ctgcccgcca ccgaaggccg tcgcagcggc ccgctgcctc cctcctacgg
     5701 cgaggacgag ttcggcttct acctgcggga tttcggatgg tatttcgcgc tcagcgacta
     5761 catggatctt cagctccagg gaagcctgtg gacccgcggc agcgtcgaga tccgcgccct
     5821 gtcccggtac cggaagcggt acgggtacga cggccaggtc cagatcagcc tgggccgatt
     5881 ccgcagcggt gaacggggcg accccgactt ctcgatccgg cgcacgggat cgctgcgctg
     5941 gacccacggc cagcccatca gcccgaccgc gaacttcgat gcgaacgtga acctgtcatc
     6001 gtccggatac ctccgcgggg tctcgcagga ctacgacgac c
//
LOCUS       DBNHQZ010000003         3576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1490709, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000003 DBNHQZ010000000
VERSION     DBNHQZ010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1490709"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16YU_00095"
     CDS             complement(<1..310)
                     /locus_tag="AC16YU_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HIR6063649.1"
                     /translation="MPDPAILTVTTDFGARDAYVAAVKGVVLSLEPGLRIVDVTHEIG
                     PQDVMQAAFVLRGVIPFFPPGTVHLVVVDPGVGTDRRAIAARKDGQLFVGPDNGLFSL
                     L"
     gene            424..1062
                     /locus_tag="AC16YU_00100"
     CDS             424..1062
                     /locus_tag="AC16YU_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR6063650.1"
                     /translation="MNDRENTNRGARASASGPNLTERHVEALASFIRFWGEMASRWGI
                     NRTMAQIHALLYAVEEPLDTDTIMERLRISRGNANMNLRSLLNWNLLRKVRLPGSRKD
                     YYAAEKDVWEITLQIIRERERREIRPVVQQLQECRDQLTGGGDRPCTASTDPQEQRFC
                     ERIDNLIKLMLVFEGLSRTLLPFIQVRNADLLRQLAAMAEPPDGYPEGPDHA"
     gene            1055..1444
                     /locus_tag="AC16YU_00105"
     CDS             1055..1444
                     /locus_tag="AC16YU_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6063651.1"
                     /translation="MRESPSETGRTGEALAAAYLEERGFRVMDRNYRFERSEVDIVCF
                     LPAPRYEDGGELVFVEVKTRRGTGFGLPEEAIGAEKKRHLVKAARAWLYERRMEGTPC
                     RFDVVSILLRSRAEPEIHHIENAFWIF"
     gene            complement(1491..3455)
                     /locus_tag="AC16YU_00110"
     CDS             complement(1491..3455)
                     /locus_tag="AC16YU_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6063652.1"
                     /translation="MPDSPRQDFPFGQRIAWTPDPDWIAESNLRAFMDRHGIPDLDAL
                     QARSVNEIEWFWSAVLEDLGIRFRTAPSRSVDLSTGPEFPAWCPGARMNILDSLLGRW
                     QAGPEADRTALRWEAEDGRTVVLTYRELSDQVGRCMTALSAMGLGRGDAVGLLMPMVP
                     EIVVAFLAIIGGGGIVLPLFSGYGPEAVATRLRDAGARALFTADGFLRRGAAVRLKPV
                     ADEAVALCPTVERVIVVRHLEDDDTPMSDGRDAWWHDTVPRHAPGAAYAELDAEDVLM
                     VIYTSGTTGKPKGAVHTHCGFPVKAAQDMAHAMDVKAGNVVWWMTDMGWMMGPWLVFG
                     TLALGATMVLYDGAPDRPGPDRTWDVVARHGVTHLGLSPVLVRTLRPHGEESVRRHDL
                     SCLRAVASTGSPWDPESWTWLFRTVLEGRKPILNYSGGTEISGGIVCGNFFRPLKPCA
                     FSGPVPGMDADVVDDSGNSVRGQVGELVIRKPWIGMTRGFLNDKGRYLETYWSRFEGV
                     WLHGDFAAIDEDGLWYILGRSDDTIKIAGKRVGPAEVESVLNAHPAIQESAAIGVPDP
                     VKGEVLVAFCVPRSEADDRLRADLVELVTTHLGKPMRPKAILFADALPRTRNAKIMRR
                     VIRAAHLGLDPGDVTSLEDPSTVEAIRRAW"
BASE COUNT          598 a         1195 c         1245 g          538 t
ORIGIN      
        1 atagcagcga aaacagtccg ttgtccggac cgacgaacaa ctggccgtcc ttgcgggctg
       61 ctatggccct ccgatccgtg ccaacgcccg gatcgacgac cacgagatgg accgtgccgg
      121 gcgggaagaa gggaatcact ccgcgcagca cgaaggcggc ctgcatgacg tcctgcggcc
      181 cgatctcgtg cgtgacatcc acgatccgga ggccgggctc gagggaaagt acgaccccct
      241 tcacggcggc gacgtacgcg tcgcgggcgc cgaaatcggt cgtgaccgtg agaatggctg
      301 gatcgggcac aggtcgaaag gcgaccggat ggtatactct ggagtcctcg ccgggccgga
      361 acagggctcc ggaacggccg tctgaaacta cggattcccg tgctggctaa gcacccggac
      421 ccgatgaatg atcgcgagaa cacgaaccgg ggcgcacggg cgtccgcgtc gggtccgaac
      481 ctgacggaac ggcacgtcga ggccctggcc agcttcatcc gcttctgggg ggaaatggcc
      541 tcgaggtggg gcattaatcg cacgatggct cagattcacg ccctgctgta cgccgtggag
      601 gagccgctcg acacggacac gatcatggag cggctccgga tcagccgggg aaacgccaat
      661 atgaacctgc ggtcgctcct gaactggaac ctgttgcgca aggtgcggtt gccgggctcc
      721 cgcaaggact attacgcggc ggagaaggac gtttgggaga tcacgctgca gatcatccgc
      781 gagcgggagc gccgtgagat caggccggtg gtgcagcagc tgcaggagtg ccgcgaccag
      841 ctgaccggtg gcggggaccg tccctgcacg gcctcgaccg acccgcagga acagcggttc
      901 tgcgagcgca tcgacaacct catcaagctc atgctcgtct tcgagggact ctcgcggacg
      961 ctcctgccct tcatccaggt ccgcaatgcc gatctgctgc gacagctcgc cgccatggcc
     1021 gagcctcccg atggctatcc ggagggaccg gaccatgcgt gagagcccat cggaaaccgg
     1081 ccggaccggc gaggccctcg cggccgcgta cctggaggag cggggattcc gggtgatgga
     1141 ccgtaactac cggttcgagc ggtccgaggt cgacatcgtg tgcttcctgc ccgctccgcg
     1201 ctacgaggac gggggcgagc tggtcttcgt cgaggtcaag acccgccggg ggacgggatt
     1261 cgggctgccc gaggaggcga tcggggcgga gaagaaacgt cacctggtga aggcggcgcg
     1321 ggcatggctg tacgagcgcc gcatggaggg cacgccctgc cggttcgacg tggtgtccat
     1381 cctgctcagg agcagggcgg agccggagat ccatcacata gagaacgcct tctggatctt
     1441 ctgaccggcc ggccggcgaa ccggccggtc cggatcgctg ccccgggacc tcaccacgcg
     1501 cgccggatcg cctcgacggt cgacgggtcc tcgaggctgg tcacgtcgcc gggatcgagc
     1561 cccaggtgcg ccgcgcggat tacccgtctc atgattttcg cgttccgggt ccggggcagg
     1621 gcatcggcga acaggatggc cttcggccgc atgggcttgc cgaggtgggt cgtcacgagc
     1681 tccacgaggt cggcccggag ccggtcgtcg gcctcggagc gcggcacgca gaaggccacg
     1741 agcacctcgc ccttgacggg atcggggact ccgatggcgg cgctctcctg gatggccgga
     1801 tgggcgttca gcacggactc gacctcggcc ggcccgacgc gcttgcccgc gatctttatc
     1861 gtgtcgtcgc tgcggcccag gatgtaccag agtccgtcct cgtcgatggc cgcgaaatcg
     1921 ccgtgcagcc agactccctc gaagcgcgac cagtaggttt ccaggtaccg gcctttgtcg
     1981 ttcaagaatc cccgggtcat tccgatccag ggcttccgga tcactagctc cccgacctgc
     2041 ccgcgaacgg agttcccgga gtcgtccacc acgtcggcat ccatgccggg aacaggcccc
     2101 gaaaaggcgc acggcttgag cggcctgaag aaatttccgc agacgatgcc ccccgagatc
     2161 tcggtcccgc ccgagtaatt gagaatgggc ttgcggccct ccaggaccgt gcgaaagagc
     2221 caggtccagg attcgggatc ccacgggctt cccgtcgagg cgaccgccct caggcaggag
     2281 aggtcgtgcc ggcgcacgga ctcctcgccg tgggggcgca gggtccggac gagcacggga
     2341 gagaggccga ggtgcgtgac gccgtgccgg gcgacgacat cccaggtccg gtccgggccc
     2401 gggcggtcgg gggcgccgtc gtagagcacc atggtcgctc cgagcgccag ggtcccgaag
     2461 accagccacg gacccatcat ccagcccatg tcggtcatcc accagaccac gttcccggcc
     2521 ttcacgtcca tggcatgcgc catgtcctgc gccgccttca ccggaaagcc gcagtgcgtg
     2581 tggacggcgc ccttgggctt tccggtcgtg ccgctggtgt agatgaccat cagcacgtcc
     2641 tcggcgtcca gttcggcgta agccgcgccg ggtgcatgcc ggggcacggt gtcgtgccac
     2701 caggcatcgc ggccgtcgga catgggcgtg tcgtcgtcct cgagatgccg gaccacgatg
     2761 accctctcga ccgtcgggca gagcgcgacg gcctcgtcgg ctaccggctt cagccggacg
     2821 gcggcgcccc ggcgcaggaa cccgtccgcg gtgaacaggg cccgcgctcc ggcgtcccgc
     2881 agccgggtcg ccacggcctc cggtccgtac ccggagaaga ggggcaggac gatgccgccg
     2941 cctccgatga tcgccaggaa ggccacgacg atttccggca ccatgggcat gagaaggccc
     3001 acggcgtcgc cccggcccag ccccatcgca gagagggccg tcatgcacct ccccacctgg
     3061 tcagagagct cccggtacgt gaggaccacg gtgcgcccgt cctcggcctc ccaacgcagg
     3121 gccgtccggt cggcttcggg gcccgcctgc cagcggccga gcaggctgtc gaggatgttc
     3181 atccgggccc cggggcacca cgcgggaaac tccggcccgg tggacaggtc cacactgcgg
     3241 ctcggcgccg tccggaaccg gatgccgagg tcctcgagga cggcgctcca gaaccactcg
     3301 atctcgttca cggaccgcgc ctggagtgcg tccaggtccg gtatgccgtg gcggtccatg
     3361 aaagccctga ggttgctctc cgcgatccag tctggatcgg gcgtccaggc gatgcgctgg
     3421 ccgaacggga agtcctggcg gggcgaatcc ggcatgggga acggggcgac ttcggaccgt
     3481 gaatacgggg cggaaccggg gccgcggaca agataatctg tccgtggtgc gtattttgtt
     3541 tcaacgagca ggtcgtatcc ggccgtgcgt ggacac
//
LOCUS       DBNHQZ010000004         4394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3658775, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000004 DBNHQZ010000000
VERSION     DBNHQZ010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3658775"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="AC16YU_00115"
     CDS             <1..563
                     /locus_tag="AC16YU_00115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6063653.1"
                     /translation="EVSEWVAATELTELDRIRLNPVFGVGNPDADLMVIGEAPGADED
                     RQGEPFVGRAGQLLTKILEAIGFARGDVYIANILKSRPPNNRDPLPSEVQAHIPILYR
                     QITLIRPKVLLCVGRTAGNSLLGRDEPLARLRGQFHDFHGLPVAVTYHPAALLRNGQW
                     KRPTWEDVQTVRRRYDELAGATPSLA"
     gene            560..2050
                     /gene="dnaB"
                     /locus_tag="AC16YU_00120"
     CDS             560..2050
                     /gene="dnaB"
                     /locus_tag="AC16YU_00120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6063654.1"
                     /translation="MSESAGNGSSRLPSIDLGGRMPPQAVDVEQAVLGAMLIEREAIP
                     RAIEILPPDSFYSAKHTRIYEAMLSLFERGNPVDIITVTEELRRREHLDEVGGAYYVS
                     ELTSRVANAANVEYHARIIAEKSLVRKLIETMSGLIGDAYDPGSDAFELLDRAEGEIF
                     RISDTQLRRSASSLQEVLKETVTRLQAIHGREGGVTGVPSGFGRLDDMTGGWQPSDLI
                     IIAARPSMGKTAFALAAARNAALHPQTPTPVAIFSLEMSAQQLAQRMLTSEARVDAQA
                     ARTGRMDDEDWPRLARSAGRLSEAKIFIDDTPGLTILELRAKCRRLKAEHDIGLVVVD
                     YLQLMHATAMSRNANREQEIAHISRSLKSLAKELNVPVLALSQLSRAVETRGGDKRPQ
                     LSDLRESGSIEQDADVVTFIYRPERYGITVDEHGNSTEGLAEIIIGKQRNGPIGDVRL
                     AFVHQHARFENLSSYYGGQRPRSEAEVSGAGREFRGAGAEDAEAPF"
     gene            2088..3266
                     /locus_tag="AC16YU_00125"
     CDS             2088..3266
                     /locus_tag="AC16YU_00125"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6063655.1"
                     /translation="MIDPKAFYRALDSLLARIRLQGDDRSFLPDILTAFQEAFGPTLH
                     FGNGRLYDERDGSFDLVAGARGQVRLGRDHPAIAAVLEHGSYVFERPDERDWFDGGGD
                     GVPAAFSVWQTTTRRWIFVYELGEGWSHEEVAFAMNAVRFALNNRLQAEAVRSDVEQA
                     AEIQRSLLPSDVPKFEGFEFAARSIPAESVGGDFFDFIDLGSDVVGVSIGDASGHGLP
                     AALLVRDVMTGLRMGMEAQLKMLHTLKKLNRVVHRSAWSSRFLSVFYGEIEKNGNILY
                     ANAGHTSPVLVSASAVHRLEPTGTVFGPLPEIHLERNFVRMEPGSVLVLMTDGIVERF
                     DEAGDMFGLEGVEELVRANLSLTATELIDLVFAEVRAFGGSAPLADDSTLVVIRRLAT
                     "
     gene            complement(3282..3686)
                     /locus_tag="AC16YU_00130"
     CDS             complement(3282..3686)
                     /locus_tag="AC16YU_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063656.1"
                     /translation="MSSSFERDGWDSGRSPSVSRDKWVRFAIQKMERGYTLIISDARR
                     NASFFLSGKGYEGCPYRAAKQLVLDGLVKEAGRHPLGCQYALDPDAVARLDEPPPKTP
                     IKGRRRFDPDPDLRPGKDHRPDRTNEEEPTDE"
     gene            complement(3728..>4394)
                     /locus_tag="AC16YU_00135"
     CDS             complement(3728..>4394)
                     /locus_tag="AC16YU_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6063657.1"
                     /translation="SILSGRLPVAQRARARFLGLALALFGRVALLLSLAWIMRLTSPL
                     FEILGEAISGRDIILIGGGLFLLAKSTHEIHAKLEGEEEPGAARAGQATFRSVLIQIF
                     VLDLVFSLDSVITAVGMVDEVPIMITAIVIAILFMMVSASAVSRFIEQHPTVKMLALA
                     FLLMVGVALIAEGLDLHIPRGYIYFSMAFSIFVEMLNLRLRGRRTAPVELRGTPGPGD
                     VPA"
BASE COUNT          713 a         1535 c         1490 g          656 t
ORIGIN      
        1 ccgaggtgtc ggaatgggtg gcggccaccg agctcaccga gctcgaccgg atccgcctca
       61 accccgtgtt cggcgtgggg aatccggatg cggacctcat ggtgatcggc gaggctccgg
      121 gcgcggacga ggaccggcag ggcgagccct tcgtggggcg cgccgggcag ctcctcacga
      181 agatcctcga ggccatcggc tttgcccgcg gggacgtgta catcgcgaac atcctcaaga
      241 gccggccgcc gaacaaccgc gacccgctcc cgtccgaggt ccaggcccac attccgatcc
      301 tgtaccggca gatcacgctg atccggccca aggtgctcct gtgcgtcggc cgcacggccg
      361 gcaactcgct cctgggtcgc gacgagccgc tggcccggct ccgggggcag ttccacgact
      421 tccacggact gcccgtcgcc gtgacgtacc acccggccgc cctgctgcgc aacggccagt
      481 ggaaacggcc gacctgggag gacgtacaga ccgtccggcg ccgctacgac gaactcgccg
      541 gagccactcc ttccctcgca tgagcgaatc tgccggaaac gggtcctcgc gcctcccctc
      601 gatcgacctc gggggacgca tgcctcccca ggccgtggac gtggagcagg cggtgctcgg
      661 cgcgatgctc atcgagcgcg aggcgattcc gagggccatc gagatcctgc cgcccgattc
      721 gttctattcg gcgaagcaca cccggatcta cgaggccatg ctgtcgcttt tcgagcgcgg
      781 caacccggtc gacatcatca cggtcaccga ggagctccgg cgccgtgaac acctggacga
      841 ggtgggcggc gcgtactacg tctccgaact cacctcccgg gtcgcgaacg cggcgaacgt
      901 cgagtaccac gcccgcatca tcgcggagaa gtcgctggtc cggaagctga tcgagacgat
      961 gtccggcctg atcggcgacg cctacgaccc gggctccgac gccttcgagc tcctggaccg
     1021 ggcagagggc gagatcttcc gcatctcgga cacgcagctc aggcgctctg cgtcctcgct
     1081 ccaggaggtg ctgaaggaga cggtcacccg gctccaggcc atccacggcc gggagggcgg
     1141 cgtgacgggc gttccgagcg gcttcggccg cctggacgac atgaccgggg gctggcagcc
     1201 gtcggacctg atcatcatcg ccgcgcggcc ctcgatgggc aagaccgcct tcgccctcgc
     1261 agccgcccgg aacgcggcgc tgcatccgca gacgcccacc ccggtggcca tcttctcgct
     1321 cgagatgagc gcgcagcagc tcgcgcagcg catgctcacc tccgaggccc gtgtcgacgc
     1381 ccaggcggcc cgaacgggtc gcatggacga cgaggactgg ccgaggctcg cccgatcggc
     1441 cgggcgcctg tccgaggcga agatcttcat cgacgacacg ccggggctca ccatactgga
     1501 gctgcgggcc aagtgccggc ggctcaaggc cgagcacgac atcgggctcg tcgtcgtcga
     1561 ctacctgcag ctcatgcatg ccacggccat gagccggaat gcgaaccggg agcaggagat
     1621 cgcccacatc tcgcgctcgc tcaagtcgct ggccaaggag ctgaacgtgc cggttctcgc
     1681 gctctcgcag ctgagccgcg ccgtcgagac gcgcggcggc gacaagcggc cccagctctc
     1741 ggacctgcgc gagtccgggt ccatcgagca ggacgccgac gtcgtgactt tcatctaccg
     1801 gcccgagcgc tacggcatca ccgtggacga acacggcaac tcgaccgaag gcctcgccga
     1861 gatcatcatc ggcaagcagc gcaacggccc gatcggggac gtgcgcctcg ccttcgtgca
     1921 ccagcacgcc cggttcgaga acctctccag ctactacggg ggacagcgtc cccgctccga
     1981 agccgaggtg tccggcgcgg gccgcgagtt ccggggggcg ggcgccgagg acgccgaggc
     2041 gccgttctga tcggccctca ccgaagtccc tcttccaacg cacccgaatg atcgatccca
     2101 aggcgtttta ccgcgctctc gactcgctgc tggcccgcat acggctccag ggtgacgacc
     2161 ggagcttcct gccggacatc ctcacggcct tccaggaggc cttcggaccg acgctccatt
     2221 tcggcaacgg gcgcctgtac gacgagcggg acggcagctt cgacctggtg gccggcgccc
     2281 ggggccaggt gcggctcggc cgcgatcacc cggcgatcgc cgccgtgctc gagcacggca
     2341 gctacgtgtt cgagcgtccg gacgagaggg actggttcga cggcggcggt gacggggttc
     2401 cggcggcctt ctccgtctgg cagaccacga cccggcggtg gatcttcgtc tacgaactcg
     2461 gtgagggctg gtcccacgag gaggtggcct tcgccatgaa cgcggtccgc ttcgcgctca
     2521 acaaccggct gcaggccgag gccgtccgca gcgacgtcga gcaggcggcc gagatccagc
     2581 gcagcctgct cccgtcggac gtaccgaagt tcgagggatt cgagttcgcg gcgaggtcga
     2641 ttccggccga aagcgtcggc ggggacttct tcgacttcat cgacctcggc agcgacgtcg
     2701 tgggcgtgtc gatcggggac gcgagcggac acggtctgcc cgccgccctg ctcgtgcgcg
     2761 acgtgatgac cggcctgagg atggggatgg aggcgcagct caagatgctg cacacgctga
     2821 agaagctgaa ccgcgtcgtg caccgcagcg cgtggtcgag ccgcttcctg tcggtcttct
     2881 acggcgagat cgagaagaac ggcaacatcc tgtatgccaa cgccgggcac acgtcgcccg
     2941 tgctggtctc ggcctcggcg gtccaccggc tcgagcccac gggcaccgtg ttcggcccgc
     3001 ttccggaaat ccacctggag cgcaatttcg tgcgcatgga gcccggctcg gttctcgtcc
     3061 taatgaccga cggcatcgtc gaacgcttcg acgaggccgg cgacatgttc ggcctggagg
     3121 gggtcgagga gctcgtgcgc gcgaacctct cgctcacggc cacggagctc atcgacctcg
     3181 tgttcgcgga ggtgcgggcg ttcgggggct ccgcgcccct cgcggacgac tcgaccctgg
     3241 tcgtgatccg gcggctggcc acgtaggcgg ccgctccggc ctcactcgtc cgtgggctcc
     3301 tcctcgttcg tgcggtccgg gcggtggtcc ttcccggggc gcaggtccgg gtccggatcg
     3361 aaccgccggc gtcccttgat gggcgtcttc ggcggaggtt cgtccaggcg ggccacggcg
     3421 tcgggatcca gtgcgtactg gcacccgagc gggtgacggc ccgcctcctt caccagaccg
     3481 tccagcacca gttgctttgc cgcccggtag ggacagccct cgtacccctt gccggacagg
     3541 aagaacgagg cgttccggcg cgcgtccgag atgatcagcg tgtagccgcg ctccatcttc
     3601 tggatcgcga accgcaccca cttgtcacgc gagacggacg gagatcgtcc ggaatcccat
     3661 ccgtcacgtt cgaacgaact gctcatctac gcatgtctcc acgttggatg attctcggga
     3721 ggcggcctca ggcgggcacg tctccgggtc cgggtgtgcc ccgcagctcc accggcgcgg
     3781 tccgcctgcc gcggaggcgc aggttcagca tctcgacgaa gatcgagaag gccatcgaga
     3841 agtagatgta gccccgggga atgtgaaggt ccaggccctc ggcgatcagc gcgacgccca
     3901 ccatgaggag gaaggccagc gcgagcatct tgacggtcgg atgctgctcg atgaacctcg
     3961 agacggccga ggcgctcacc atcatgaaca ggatggcgat gacgatggcg gtgatcatga
     4021 tgggcacctc gtccaccatg cccaccgccg tgatgacgga atcgagcgaa aacacgaggt
     4081 ccagcacgaa gatctggatc aggacggagc ggaaggtcgc ctgcccggcc cgggcggcgc
     4141 cgggctcctc ctcgccctcc agcttcgcgt ggatctcgtg cgtgctcttg gccaggagga
     4201 acaggccgcc gccgatcagg atgatgtcac ggccggagat cgcctcgccc aggatctcga
     4261 agagcggcga ggtcaggcgc atgatccagg ccagcgacag cagcagggcc acgcggccga
     4321 agagcgccag ggccaggccc aggaaccgcg cgcgagccct ctgggccacg ggcagccgcc
     4381 ccgagaggat cgaa
//
LOCUS       DBNHQZ010000005        10823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1897857, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000005 DBNHQZ010000000
VERSION     DBNHQZ010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1897857"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC16YU_00140"
     CDS             complement(<1..357)
                     /locus_tag="AC16YU_00140"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR6063658.1"
                     /translation="MLKGIDPCIGPGLLGTLAEMGHGDEIVLADAHFPAHALHGRVIR
                     ADGVGIPTLLDGILRLLPLDTYDDPLAMMAPVPGDTLDPEVEAGYLGIVRRHEPGAPV
                     PVRVERFAFYGRARAAY"
     gene            528..1370
                     /locus_tag="AC16YU_00145"
     CDS             528..1370
                     /locus_tag="AC16YU_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063659.1"
                     /translation="MRRLIVIAFALAALPARAQDTTAVARDVAALRARGVVAEIRSRT
                     TKRNPETGRSTIATIEAYTRGVLADAQGGLVFFLADDDEGWIRPNAVALLEPADSLIG
                     LLEGEGRDASALRELNDQWFPHLEDGGDAALIRAWEEDLLEQGVPLVISMFTFERSDA
                     GDVFPSFGVKNISRRTVEGVALEMIGFNQYGDVVRDKSTGMATHYATLTGTIRPGDVA
                     LFSYTDTALWSNRSTTCIEIRKMVVTFQDGSSATVDYQLKEARMSPDTYHVMGECSRA
                     GSGP"
     gene            complement(1379..2743)
                     /locus_tag="AC16YU_00150"
     CDS             complement(1379..2743)
                     /locus_tag="AC16YU_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR6063660.1"
                     /translation="MPRLVLVLALVLMSSGASAQSPAEKVEAHRADAERLIEAALADS
                     AAWNRLAELTDTFGPRFSGTQALEDALGWILDAMRADGLENVRGEPVMVPRWVRGRES
                     ATLVEPRAQDLAMLGLGGSVSTPADGITAEVLVVSSFDELAARSGEAAGRIVLYDVEW
                     QGYGRTVQYRSRGAVEAARVGAVASLIASVGSGSLYTPHTGGMGYEEGVPRIPHAAIT
                     IEDSRMLRRMQDRGQRIVVRLDMEAHFLPDAPSRNVIAEVVGSEFPDQVVVLGGHSDS
                     WDVGQGAMDDGGGCVAAWEAVRLIKALGLRPKRTVRVALWTNEENGLRGGTAYRDAHR
                     PALGDHLLAIESDGGVFRPSGMGFTGSDEAFEILQAIGTLLEPIGAGTMTRGGGGADI
                     GPVMNEGVPGASPNVDESRYFDYHHTHADTMDKLDPRDVALNVAMLAVVAYTVANWDE
                     RLPR"
     gene            2841..4328
                     /locus_tag="AC16YU_00155"
     CDS             2841..4328
                     /locus_tag="AC16YU_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR6063661.1"
                     /translation="MAPFDAADRLFDAAGIARILLLCCAVSACGSDPPLLPESMPFSW
                     NQDARWEAYEASFRDARTAGCPGVEPALEALLERISATADSVHVHGADPDAAAFDRLE
                     EDVFSAAPLAAVCRGLLPAYQGAYSAARDAVKRASRDWDMDDRLHRERLYRLLYGGRA
                     ALEEAMLQGPLGDAEPVLFGRADVQPWLPAATVRGVTLQSGDLLLSRGGAASSALIAR
                     GNDFPGNFSHVALVRVDDATGEPSVVEAHIERGVVPADAEAYLADHKLRILVLRLRPD
                     HPALRNDPDLPRRAADLALDEARRRHIPYDFALDFVDPERMFCSEVASWAYREQGMGL
                     WMGLSTLSAPGLVRWLHQVGVRHFETHEPSDLEYDPQLQVVAEWLDPETLWKDHVDNA
                     AIDVMLEGADAGEDLGYPVWMVPVAAVMKAWSWTLNRFGGVGPVPEGMGALTALRVRD
                     LTARHDALVRSTLRLAEAYEAEHGYRPPYWDLVGLAREAKAAGPR"
     gene            4370..6625
                     /locus_tag="AC16YU_00160"
     CDS             4370..6625
                     /locus_tag="AC16YU_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR6063662.1"
                     /translation="MSTVSAAASTPTAPRGHGFGTAPVFFAGLSTILGAVMFLRFGYA
                     VGHVGLAGALLIVLLGHMVTVPTALALAEIATNRRVEGGGEYFIISRSFGTTIGSVIG
                     VSLYLSQAVSLAFYMIAFAEAFTLFAPDFERLTGLAFDARYVSLPATLLLVLTIYLRG
                     AHGGVRLLYVVVAVLAVAITAFFLGGPVEGADPEALPVMSGIADADPFILVFAVCFPA
                     FTGMTAGVGLSGDLANPRRSIPRGTLGATITGMLVYVAIIWKLAASATPEQMAEDQLI
                     MARIAIWGPIIPIGLACATLSSAIGSVLVAPRTLQAIGKDGALPWGGMSRWLSAGVGD
                     VNEPRNTTLVTGAFALIVVLAGNVDIVARLISMFFMVTYGSLCAISLLEHFAARPGYR
                     PSFRSRWYFSLLGAVMSLLLMFQMDPLFALLAIATLAGLYAVMTRSGGASKDDLAAMF
                     EGVMTQLNRNFHLRLHRRDDRQSGDEWRPSIISVNERTFDAQAPLALMRWLCHRQGFG
                     TYLHYIPGVLDEKSFHESEVVRSRLVQLARAQNTSVYMDTVVSPSMTSALAQSLQLPG
                     ISGLPNNTVLFEISAHDDPARISEVVNHVRFASATRMTLLVLRHGDYFFGDRRSIHVW
                     LTWNDATNANTMILLGYILVGHPDLSHSEIRVYVALPTGEVDAHRTRLESSVQTGRLP
                     VAEHNIRYLPVDSEESFRRTILETSRDADLTIVGFDLDGLRERGPDVFTNHPGLRDVL
                     FVNAQESIRLD"
     gene            6768..8057
                     /locus_tag="AC16YU_00165"
     CDS             6768..8057
                     /locus_tag="AC16YU_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6063663.1"
                     /translation="MPRLALVVPVPLVAFLVLLAVSPGGRAPSALPGADDEACVTCHE
                     EVTPGVVGDWRASRHAAEGTTCPDCHGDGHTSAEDVLEVELPTPETCETCHSERVEQY
                     ARGKHALAWAAMKAMPTLHWQPAGLIQGLKGCGGCHKIGLKTDEEIVDLKLEGHRYGV
                     ASCDACHTRHTFSVAEARQPEACQTCHMGFDHPQWEMYSSSKHGVRHGLKRDGVLPET
                     AAAPTCQTCHMPGGDHAVGTSWGFLAVSLPMPDDERWTADRTTILKGLGVLAPDGEPT
                     ARLQAVRDARLARLDYAEWFDLREKMVAVCTGCHAETYARTELAKGDDQIREIDHLMA
                     EAIRVVAGLYEDGTIAKPESYAAAFPDLLTFHDAATSIEQRLFVMFLKHRMRAFQGAF
                     HMNPDYALWYGWSEMVMDLTDIREMADEMRFQRRLGE"
     gene            complement(8058..9374)
                     /locus_tag="AC16YU_00170"
     CDS             complement(8058..9374)
                     /locus_tag="AC16YU_00170"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6063664.1"
                     /translation="MIRLAALLLLLLVSAAPAVTAQTSAILAGHLVDPESGTAAADQV
                     ILVERDPATGRSRILAVGADVAIPDGAEVIDLSDQWVLPGLVDAHDHLGITYKEDPES
                     WYYYFTVIMDSTPLRAIQSFSTGFQKLAHGFTIVRDLGNNGLYADTALRQAIEMGWVP
                     GPTIINSGIIIGGFGGQLFETPEREDTVYPEYLNADTDDEIVKAIRRNIHYGGKVVKV
                     CVDCQAYPYTVEQLELFVREAANAGQRVSGHVQTVEGARRAAEAGLWSLDHDNALTPE
                     IHAIMAEKGIFRAGTETPKASWSRVTDAQWERRLAGLRDAYEKGVPLVFSTDADYFIP
                     GMNRGELTIEFIETWKAAGIPDAEILKVMTINGYRISETMETRGPIRVGLAADIIATA
                     ANPLEGIDALRDVRFVMKDGLVFKRDGVVAPLDFFHNGPAYGWRKR"
     gene            9540..10016
                     /locus_tag="AC16YU_00175"
     CDS             9540..10016
                     /locus_tag="AC16YU_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063665.1"
                     /translation="MESSSSSSEFRSSPTVSKEKWAMHARAKLLKGYVLIIADGRRTA
                     SFFKEGRGFEPCPFKIAKALVEMGLVEEAGTHFLGTRFVLSSAAPVPPPVVEPDEDDE
                     TDDAAPREQAATGMEGLLDDLEAEQTDDEEEGEEAAGEEESDAEEAEEDEEEEEDY"
     gene            complement(10010..>10823)
                     /locus_tag="AC16YU_00180"
     CDS             complement(10010..>10823)
                     /locus_tag="AC16YU_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6063666.1"
                     /translation="DDAKSNFVAASRLGLDSQLAWVDGNRWPAHDLVLRELLPTAREG
                     LQSSGIDAADIDRYLGVIEERVGSRMTGSQWQLASLARMKKKPSTRAERLDALVAAMR
                     NRQVQGLPGHRWALAELEEGPEIERLPSSRVEHFMTTDLFTVHEDEAVEFVAVLMDWR
                     RIRHVIVEDRRHRLVGLVPHRRLLRYLAERESRPELVGDVTVKDIMIADPVSVSPETT
                     TMEAIRLMRDRSIGALPVVRDGQLVGMVTEVAFARIAGKILDQTLARGPGTQ"
BASE COUNT         1615 a         3820 c         3669 g         1719 t
ORIGIN      
        1 gtacgccgcc ctcgcccgtc cgtagaaggc gaagcgctcc acgcgcacgg gcacgggcgc
       61 tcccggctcg tgacgccgga cgatcccgag gtacccggcc tccacttcgg ggtcgagcgt
      121 gtcgcccggg acgggggcca tcatcgccag cggatcgtcg tacgtgtcga gggggagcag
      181 gcgcaggatg ccgtcgagaa gggtcggaat gccgacccca tccgcgcgga tcacgcgccc
      241 gtgaagcgca tgcgccggga aatgggcgtc cgccagcacg atttcgtcgc cgtgccccat
      301 ttcggcgagc gtcccgagga gcccggggcc gatgcacgga tcgatgcctt tcagcacggg
      361 tcgtaccgtt tcttgcgccg accgaaggta cgctccctgc gtccttttcc gctcggtcgc
      421 tgtagggatc cgtccctcag ggtggcgccg acaagtcccg cttgaggcgg gccccttccc
      481 tcgcgtcatt gtcgttcgga ccgcatgtct acctgcgagg tgacacgatg agacggttga
      541 tcgtgatcgc attcgcgctt gccgccctgc cggcgcgggc ccaggacacg accgctgtgg
      601 cgcgggacgt ggccgccctt cgagccaggg gcgtggtcgc cgagatccgc tccaggacga
      661 ccaagcgcaa cccggagacc ggacgctcga ccatcgccac gatcgaggcc tacaccaggg
      721 gcgtgctcgc ggacgcgcag ggcggcctgg tctttttcct ggccgatgac gacgagggtt
      781 ggattcgccc gaacgccgtc gccctgctcg agccggccga ctcgctgatc ggcctcctcg
      841 agggcgaggg acgcgacgcg tcggcgctgc gcgagctcaa cgaccagtgg ttcccccacc
      901 tcgaggacgg gggtgacgcc gccttgatca gggcctggga ggaggatctc ctcgaacagg
      961 gtgttccgct cgtcatctcg atgttcacgt tcgagcggtc ggacgccggc gacgtcttcc
     1021 cgtccttcgg cgtgaagaac atctcccgac ggaccgtcga gggcgtcgcg ctcgagatga
     1081 tcggcttcaa ccagtacggc gacgtggtgc gcgacaagtc gaccggaatg gccacccact
     1141 acgccaccct caccgggacc atccggccgg gcgacgtcgc gctcttcagc tacacggaca
     1201 ccgccctgtg gtccaaccgg tccacgacct gcatcgagat ccggaagatg gtcgtcacct
     1261 tccaggacgg ctcgtctgcg accgtggatt accagctgaa ggaggcccgc atgtcgccgg
     1321 acacctacca cgtcatgggc gagtgcagcc gggcggggtc ggggccgtaa ccgggcggct
     1381 accggggcag ccgctcgtcc cagttcgcga cggtgtaggc cacgacagcg agcatcgcca
     1441 cgttcagggc gacgtcgcgg gggtcgagtt tgtccatcgt gtcggcgtgc gtgtggtggt
     1501 agtcgaagta gcgcgactcg tccacgttcg ggctcgcccc gggcacgccc tcgttcatga
     1561 cgggaccgat gtcggcgcct cccccacccc gggtcatggt gccggccccg atgggctcga
     1621 gcagggtgcc gatcgcctgc aggatctcga acgcctcgtc ggacccggtg aagcccatgc
     1681 cgctcgggcg gaatacgccg ccgtcggatt cgatcgcaag gaggtggtca cccagggccg
     1741 gccggtgggc gtcccggtac gccgttccgc cgcgcaggcc gttctcctcg ttggtccaca
     1801 gggcgacccg gaccgtccgc ttcggacgca ggcccagcgc cttgatcagc cggacggcct
     1861 cccacgcagc cacgcacccg ccaccgtcgt ccatggctcc ctgtcccacg tcccacgagt
     1921 cgctgtgccc gcccagcacc acgacctggt ccgggaactc ggagccgacc acctccgcga
     1981 tcacgttccg cgacggggcg tccggcagga agtgggcctc catgtccagc ctgacgacga
     2041 tgcgctgtcc gcggtcctgc atccggcgga gcatccggga atcctcgatc gtgatggcgg
     2101 cgtgcgggat gcgggggacg ccctcctcgt agcccatgcc ccccgtgtgg ggggtgtaca
     2161 gcgaaccgga gcccacgctc gcgatgaggc tggccacggc gcccacgcgg gcggcctcga
     2221 cggcgccgcg cgaccggtac tgcaccgtgc ggccgtatcc ctgccattcc acgtcgtaga
     2281 ggacgatacg gccggcggcc tcgcccgagc gtgcggccag ctcgtcgaag gacgacacca
     2341 cgagcacctc ggccgtgatg ccgtccgccg gcgtgctcac cgagccgccc agcccgagca
     2401 tggccaggtc ctgggcgcgc ggctcaacga gcgtcgcgga ctcccggccc cgcacccagc
     2461 gcggcaccat cacgggctcg ccgcgcacgt tctcgaggcc gtcggcgcgc atcgcgtcca
     2521 ggatccaacc cagcgcgtcc tcgagcgcct gcgtgccgct gaaacgcggt ccgaacgtat
     2581 cggtcagctc ggccaggcgg ttccaggcag cgctgtcggc gagcgcggcc tcgatcaggc
     2641 gctcggcgtc ggcccggtgg gcctccacct tttcggcagg cgactgggcg gaggctccgg
     2701 atgacatgag gaccagggcg agaacgagca cgaggcgggg catttgactc cgggttcagt
     2761 ggatcgggcc ccgaatctac gggatcgttc ttatcatcgc gaccaccgat cgacgccctc
     2821 ccgattcacc gcgctgcgcc atggccccgt tcgacgcagc cgaccgcctg ttcgacgcag
     2881 ccgggatcgc ccgcatcctg ctcctctgct gcgccgtgtc cgcgtgcggc tccgatccac
     2941 cactcctgcc cgaatccatg ccgttctcct ggaaccagga tgcacgatgg gaggcgtatg
     3001 aagcctcctt ccgcgatgcc cgcaccgcgg gctgccccgg cgtcgagccc gcgctcgagg
     3061 ccctgctcga acggatctcg gccacggccg actcggtcca tgtgcacggc gcagatccgg
     3121 acgcggccgc cttcgatcgc ctcgaggagg acgtcttctc ggccgccccg ctggcggcgg
     3181 tctgccgcgg gctgctaccg gcctaccagg gggcatactc cgccgcgcgg gacgcggtca
     3241 agcgggcgtc gcgggactgg gacatggacg atcgcctgca ccgtgaacgg ctgtaccgcc
     3301 tgctctacgg aggacgggcg gccctcgagg aggccatgct ccagggtccc ctcggcgacg
     3361 ccgagcccgt cctgttcggc cgcgccgacg tgcagccctg gctccccgcg gccacggtcc
     3421 ggggcgtcac cctgcagagc ggcgacctgc tgctttcgcg gggcggagcc gcgtcgtcgg
     3481 ccctgatcgc gcggggcaac gacttcccgg gcaatttctc ccacgtggcg ctcgtgcggg
     3541 tagacgacgc cacgggcgag ccgtcggtcg tcgaggccca catcgaacgg ggagtcgtgc
     3601 cggcggacgc ggaggcatac ctcgcggacc acaagctccg catcctggtg cttcgcctcc
     3661 ggccggacca tccggcgctg cggaacgatc ccgacctccc gcgccgcgcc gcggacctcg
     3721 ccctcgacga agcgcggcgg cggcacatcc cgtacgactt cgccctggat ttcgtggatc
     3781 ccgaacggat gttctgctcg gaagtggcct cctgggccta ccgcgagcag ggcatggggc
     3841 tctggatggg cctgtcgacc ctgtccgccc cggggctcgt ccgctggctc caccaggtgg
     3901 gcgtccgcca tttcgagacg cacgagccga gtgacctcga gtacgacccg cagcttcagg
     3961 tcgtggccga gtggctggat cccgagacgc tctggaagga ccacgtcgac aacgccgcga
     4021 tcgacgtcat gctcgaaggc gcggacgcgg gcgaagacct gggctacccg gtctggatgg
     4081 tgcccgtggc ggccgtcatg aaggcctgga gctggacgct gaaccggttc ggcggcgtgg
     4141 gtccggtccc ggaaggaatg ggagccctga ccgcgctccg ggtgcgcgac ctcaccgccc
     4201 ggcacgacgc cctggtgcgc tcgacgctcc ggctggccga ggcgtacgag gccgagcacg
     4261 gctatcgtcc gccctactgg gacctcgtgg gcctcgcgcg tgaagcgaag gcggccggtc
     4321 cgcggtagcc ggtcccgggc gcctatcttc cagcgctaca gcagcaccca tgtcgaccgt
     4381 atcagcagca gccagcaccc cgaccgctcc gcgcgggcac ggcttcggaa ccgcgcccgt
     4441 gtttttcgcg ggcctcagca ccattctcgg ggcggtcatg ttcctgcggt tcggctacgc
     4501 cgtggggcac gtggggcttg caggggcgct gctgatcgtc ctgctcggcc acatggtcac
     4561 cgtccctacg gcgctcgcgc tcgccgagat cgccacgaac cggcgtgtcg agggcggcgg
     4621 cgagtacttc attatctcgc gctcgttcgg gaccacgatc ggcagcgtga tcggcgtctc
     4681 gctgtacctg tcgcaggccg tgtcgctggc gttctacatg atcgccttcg ccgaggcctt
     4741 tacgctgttc gccccggatt tcgagcgcct gaccgggctc gcgttcgatg cgcgctacgt
     4801 gtcacttccg gcgacgctcc tgctcgtgct cacgatatac ctgcgcgggg cacacggggg
     4861 cgtccgcctg ctgtacgtgg tcgttgcggt gctcgcggtc gcgattaccg ccttcttcct
     4921 cggcggtccc gtcgagggcg cggacccgga ggccctgccg gtgatgtcgg gcatcgccga
     4981 cgccgacccg ttcatcctgg tcttcgcggt ctgcttcccg gcgttcacgg gcatgaccgc
     5041 gggtgtcggt ctgtcggggg acctcgccaa tccccgccgc tccatcccga ggggcacgct
     5101 cggcgcgacg atcacgggaa tgctcgtcta cgtggcgatc atatggaagc tcgccgcatc
     5161 cgccaccccc gagcagatgg ccgaggatca gctcatcatg gcccggatcg cgatctgggg
     5221 tccgatcatc ccgatcggcc tcgcatgcgc cacgctgagc tcggccatcg gatcggtgct
     5281 cgtggcccct cgcacgctgc aggcgatcgg gaaggacggc gccctgccgt ggggcggcat
     5341 gagccggtgg ctctcggccg gcgtcggaga cgtgaacgag ccacggaaca cgacgctcgt
     5401 caccggggcc ttcgcattga tcgtggtgct cgccggcaac gtggacatcg tcgcgcgact
     5461 catctccatg ttcttcatgg tgacgtatgg ctccctctgc gcgatcagcc tgctcgagca
     5521 tttcgcggcg cgccccggct accgcccgag cttccggtca cgctggtact tcagcctgct
     5581 cggcgccgtg atgagcctgc tcctgatgtt ccagatggat ccgctgttcg cgctgctcgc
     5641 catcgcgacg ctcgcgggcc tgtacgccgt catgacccgc tcgggcgggg cttcgaagga
     5701 cgacctcgcc gccatgttcg agggcgtgat gacgcagctc aaccggaact tccacctgag
     5761 gcttcaccgc agggatgatc ggcagtcggg ggacgagtgg cggccgtcga tcatctcggt
     5821 gaacgagcgg actttcgacg cgcaggcccc gctcgcgctc atgcgctggc tctgtcaccg
     5881 ccagggtttc ggcacctacc tgcactacat cccgggcgtc ctggacgaga agtccttcca
     5941 cgaatcggaa gtcgtccggt cccgcctggt ccagctcgcg cgcgcccaga acacgtcggt
     6001 ctacatggac acggtcgtga gcccatccat gacctcggca ctggctcaga gcctgcagct
     6061 gcccggcatt tcgggcctgc cgaacaacac ggtgctcttc gagatctcgg cccacgacga
     6121 cccggcccgc atctcggagg tggtcaatca cgtacggttc gcgtccgcaa cccggatgac
     6181 cctgctcgtg cttcgtcacg gcgactactt cttcggcgac cgccgctcga tccacgtctg
     6241 gctcacctgg aacgacgcca cgaacgccaa cacgatgatc ctgctcgggt acatcctggt
     6301 cggccacccg gacctgtcgc attccgagat ccgcgtatac gtggcccttc cgaccgggga
     6361 ggtggatgcc caccggaccc ggctcgagtc ctcggtccag accgggcgcc tcccggtcgc
     6421 agagcacaac atacgctacc tgcccgtcga ctcggaagaa tcgttccgcc gcacgatcct
     6481 cgagacctcg cgagacgcgg acctgacgat cgtcggtttc gacctggacg gtcttcgcga
     6541 gcgcggaccc gacgtgttca ccaaccatcc cgggctgcgt gacgtgctct tcgtgaacgc
     6601 ccaggagtcg atccgactgg actgatgggc cagtgcccat ggccggccgc aggaaggttt
     6661 ggccctacag ggctttggga cctgccccga caggcgcggg gcggacgggc gcctatgctt
     6721 tcggctgacg cgttccgcca tccaatgccc gtccggagcc cgcagccatg ccacgcctcg
     6781 ccctcgtcgt gcccgttccg ctcgtcgctt tccttgtcct gctcgccgtc tcgccgggcg
     6841 ggcgcgcccc ttccgccctg ccgggcgcgg acgacgaagc ctgcgtcacc tgccacgagg
     6901 aggtgacccc gggggtcgtc ggcgactggc gggcgagccg ccacgcggcc gaggggacga
     6961 cgtgcccgga ctgccacggc gacggccaca cgagcgcgga agacgtcctc gaggtggaac
     7021 tcccgacgcc cgagacgtgc gagacctgcc attccgagcg cgttgagcag tacgcgcggg
     7081 gcaagcacgc cctcgcctgg gcggccatga aggccatgcc cactctgcac tggcagccgg
     7141 ccggcctgat ccagggcctg aagggctgcg gagggtgcca caagatcggc ctgaagacgg
     7201 acgaggagat cgtcgacctg aagctcgagg gacaccgcta cggtgtggcc tcgtgcgatg
     7261 cctgccacac ccggcacacg ttttctgtcg cggaggcccg gcagcccgag gcctgccaga
     7321 cgtgccacat gggcttcgac cacccgcagt gggagatgta ctcgtcgtcc aagcacggcg
     7381 tgcgccacgg cctgaagcgg gacggcgtgc tcccggagac ggccgccgca ccgacctgcc
     7441 agacgtgcca catgcccggc ggcgaccacg ccgtcgggac gtcctggggc ttcctggccg
     7501 tgagcctgcc aatgccggac gacgagcggt ggacggcgga ccggaccacg atcctgaagg
     7561 gtctgggcgt cctggccccg gacggggaac ccacggcccg cctgcaggcc gtccgtgacg
     7621 cccggctcgc ccggctcgac tacgccgagt ggttcgacct ccgggagaag atggtcgccg
     7681 tgtgcacggg atgccacgcg gagacgtacg cccgcaccga gctggcgaaa ggggacgacc
     7741 agatccgcga gatcgaccat ctcatggccg aggccatccg ggtggtcgcc ggcctgtacg
     7801 aggacggcac gatcgcgaag cccgagtcct acgccgcggc cttccccgac ctcctcacct
     7861 tccacgacgc ggccacgtcg atcgagcagc ggcttttcgt catgttcctg aagcaccgca
     7921 tgcgcgcatt ccagggcgcg ttccacatga acccggacta cgcgctgtgg tacggctgga
     7981 gcgagatggt catggacctg accgacatcc gggagatggc ggacgagatg cggttccagc
     8041 gccggctcgg cgagtagcta tcgtttccgc cagccgtagg cggggccgtt gtggaagaag
     8101 tcgagcggcg ccaccacgcc gtcccgcttg aagacgagcc cgtccttcat cacgaagcgg
     8161 acgtcccgca gggcgtcgat gccctcgagc ggattcgcgg cggtcgcgat gatgtcggcg
     8221 gccagcccga cccggatcgg accgcgggtt tccatggtct ccgagatccg gtacccgttg
     8281 atggtcatca ctttcaggat ctcggcgtcc ggaatgcccg ccgccttcca tgtttcgatg
     8341 aactcgatcg tgagctcgcc gcggttcatc ccggggatga agtagtcggc gtcggtcgag
     8401 aacacgagcg gaacgccctt ctcgtatgcg tcgcgaaggc ccgcgaggcg ccgctcccac
     8461 tgggcgtccg tcacgcggga ccacgaggcc ttcggcgtct cggtcccggc gcggaagatg
     8521 cccttctcgg ccatgatcgc gtggatctcg ggcgtcagcg cgttgtcgtg gtccagagac
     8581 cacagccccg cctccgcggc gcgccgggcg ccctcgaccg tctgaacgtg accggagacc
     8641 cgctggcccg cgttcgccgc ctccctgacg aacagttcga gctgctcgac cgtgtacggg
     8701 taggcctggc agtccacgca caccttcacc accttgccgc cgtaatggat gttgcggcgg
     8761 atggccttca cgatctcgtc gtccgtgtcc gcattcaggt actccgggta taccgtgtcc
     8821 tcgcgctcgg gcgtctcgaa gagctggccc ccgaagccgc cgatgatgat gccggaattg
     8881 atgatcgtcg ggccgggcac ccagcccatt tcgatggcct gccggagcgc ggtgtcggcg
     8941 tagaggccgt tgttgcccag gtcccgcacg atcgtgaacc cgtgggccag cttctggaat
     9001 ccggtcgaga acgactggat cgcccgcagc ggtgtcgagt ccatgatgac ggtgaagtag
     9061 tagtaccagc tctccgggtc ttccttgtag gtgatgccca ggtgatcgtg cgcgtccacg
     9121 agccccggga ggacccactg gtcggagagg tcgatcacct cggcgccgtc cgggatcgcg
     9181 acgtccgcac cgaccgccag gatgcggctc cggccggtcg ccggatcgcg ctccacgaga
     9241 atcacctggt cggccgcggc agtgccgctc tccggatcca ccaggtggcc cgccaggatg
     9301 gcgctggtct gggcggtgac ggcgggggca gccgaaacga ggagcagcag gagcagggca
     9361 gcgaggcgga tcatgacgtt gtccggtgaa tcgagtcccc caagtaccga cgtgcaccgg
     9421 tcaggtgcaa caggcggcgg atcaaggaga atttgtggcg gatgcgcttg tgcggaaccg
     9481 ggaacgtggc gttatgttag gcggcgcatt tcccgaatcc ggcaccgcaa gccctgacga
     9541 tggaaagctc gtccagctct tccgaattcc gcagcagccc caccgtctcg aaagagaaat
     9601 gggccatgca cgcccgcgcc aagctgctga agggatacgt actcatcata gcggacggca
     9661 gacgcaccgc cagcttcttc aaggagggcc gcggattcga gccgtgtccg ttcaagatcg
     9721 cgaaggccct cgtcgagatg ggcctcgtgg aagaggcagg cacgcatttc ctggggaccc
     9781 ggttcgtcct ctcgtccgcc gcgcccgttc cgccgccggt cgtcgaaccc gacgaggacg
     9841 acgagaccga cgatgccgct ccgcgcgagc aggcggcgac ggggatggag ggactgctcg
     9901 acgacctcga ggccgagcag acggacgacg aggaggaagg agaagaggcc gcaggcgaag
     9961 aggaatccga cgccgaggag gcggaagagg acgaggagga ggaagaggac tactgagtcc
    10021 ccgggccccg ggcgagcgtc tggtccagga tcttgccggc gatccgggcg aaggcgacct
    10081 cggtgaccat gcccacgagc tggccgtccc gcacgacggg caatgcgccg atgctccgat
    10141 cgcgcatgag cctgatcgcc tccatcgtcg tggtctcggg tgacaccgac accgggtcgg
    10201 cgatcatgat atccttcacg gtgacgtcgc cgacgagctc gggcctggat tcgcgctcgg
    10261 ccaggtagcg gagcagccgg cggtgcggca cgagccccac gagccggtgg cgccggtcct
    10321 cgacgatgac gtggcggata cggcgccagt ccatgagtac ggccacgaat tccaccgcct
    10381 cgtcctcgtg gacggtgaag aggtcggtcg tcatgaagtg ctcgacccgc gacgagggga
    10441 gcctctcgat ctccggcccc tcttccagct ccgcgagcgc ccaccggtgg ccgggcagcc
    10501 cctgcacctg ccggttgcgc atggccgcca cgagcgcgtc cagccgctcc gcccgggtcg
    10561 agggcttctt cttcatgcgg gcgagcgacg cgagctgcca ctgggatccc gtcatgcgcg
    10621 accccacccg ctcctcaatc acgcccaggt accggtcgat gtcggcagcg tcgattccgg
    10681 acgactggag gccttcgcgg gcggtcggaa gcagctcccg caggacgagg tcgtgcgcgg
    10741 gccagcggtt gccgtccacc caggcgagct gcgagtccag gccgagccgg gaggccgcga
    10801 cgaagttgga cttcgcgtcg tcg
//
LOCUS       DBNHQZ010000006         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1626912, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000006 DBNHQZ010000000
VERSION     DBNHQZ010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1626912"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16YU_00185"
     CDS             complement(<1..451)
                     /locus_tag="AC16YU_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR6063667.1"
                     /translation="MAKQITFDSEARTALKRGVDALADAVKVTLGPKGRNVIIEKKFG
                     APTVTKDGVTVAKEIELEDKIENVGAQMVKEVASKTSDVAGDGTTTATVLAQAIMTAG
                     LKNVTAGANPMDLKRGIDKAVEAVVEALRSMSRDIEDKNEISQVATVS"
     gene            complement(499..786)
                     /gene="groES"
                     /locus_tag="AC16YU_00190"
     CDS             complement(499..786)
                     /gene="groES"
                     /locus_tag="AC16YU_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6063668.1"
                     /translation="MSSIKPLGDRVVVKPQPAEEKTASGLFIPDTAKEKPQKGKVMAV
                     GPGRVENGTKIDMTVKAGDTVLYGKYSGTEIELDGEEVLIMRETDILGIVK"
     gene            1098..1508
                     /locus_tag="AC16YU_00195"
     CDS             1098..1508
                     /locus_tag="AC16YU_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR6063669.1"
                     /translation="MVLVTAPPYTGDGVRAVLMAAFSGVCHQLPARSPHVDGVPLAVC
                     FRCYGIYWGILLAALLYPVLRRRLGSMERTAPLVLVGSLVPAGVDWLLELVGLVPGSQ
                     AVRVVTGAVFGLAAGYFLVSGIARARGQPREAGA"
     gene            1597..2085
                     /locus_tag="AC16YU_00200"
     CDS             1597..2085
                     /locus_tag="AC16YU_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 family protein"
                     /protein_id="HIR6063670.1"
                     /translation="MPSKQQSIVLGGVLVGLLSTSYLGLINLLCCLGVIIGALVAVWH
                     YTSQNELTLSAGQGAGLGALAALLGWAIAFVLNYVLIKVGVRHDLVMTQFILDRFGGS
                     MPPEQYDQMLEQMTQEMTFAKYFVNSLIGLAISALFGAIGGAIGAAVFKKGPVQDPFA
                     GV"
     gene            complement(2092..2652)
                     /locus_tag="AC16YU_00205"
     CDS             complement(2092..2652)
                     /locus_tag="AC16YU_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063671.1"
                     /translation="MDTDLLFVVALVAFYLLRQLSSRKQPTQKKPRPARPQPEAIEAE
                     PSLDDALAEIRRALGMEPPPAPPVPEVSTRPAPEAVPRNRLEPAARPLPTPVAAPMPR
                     DTFRPVETGRIEVEESFQRGLPTFRDPLQGHTHARPAAKAARAVPKAPEPKPTERLLA
                     TRRQLRDAFILKEVLGPPRSRQRRIR"
     gene            complement(2703..>3224)
                     /gene="mutT"
                     /locus_tag="AC16YU_00210"
     CDS             complement(2703..>3224)
                     /gene="mutT"
                     /locus_tag="AC16YU_00210"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HIR6063672.1"
                     /translation="LGATVCTPRTPSCDACPVRSNCAGFRSGDPTAYPLRRRRPPVPH
                     FDVAVGVVRRADGRLLIQRRPEEAMLGGLWEFPGGKCEPGERPEYACRRELREELGIE
                     VDVGDRMAEIAHAYSHFRVTLHAFPCTVRDGEPTPADGRAFAWVAREELADYAFPRAN
                     RRLIERLASGVDG"
BASE COUNT          437 a         1049 c         1170 g          568 t
ORIGIN      
        1 ccgacacggt ggccacctgg ctgatctcat tcttgtcttc gatgtcgcgg ctcatcgacc
       61 gcaacgcctc gacaaccgcc tcaaccgcct tgtcgatgcc tcgcttgagg tccatcgggt
      121 tggcgcccgc cgtcacgttc ttcaggccgg cggtcatgat cgcctgggcc aggacggtgg
      181 cggtggtggt gccgtcgccg gcgacgtcgc tggtcttgga ggcgacctcc ttgaccatct
      241 gggcgccgac gttctcgatc ttgtcctcga gctcgatctc cttggcaacg gtcacgccgt
      301 ccttggtgac ggtcggcgcg ccgaacttct tctcgatgat gacattgcgg cccttggggc
      361 cgagggtcac cttgacggcg tcggcgaggg cgtccacgcc gcgcttgagc gcggtgcggg
      421 cctccgaatc gaacgtaatc tgcttggcca tggtctattc ctgttacggt cttgttcgtt
      481 gaaagggtgg gggcgtcctt acttcacgat cccgaggatg tcggtctcgc gcatgatgag
      541 gacttcctcg ccgtcgagct cgatctccgt tccggagtac ttgccgtaga ggacggtgtc
      601 gccggccttg acggtcatgt cgatcttggt gccgttctcg acgcggcccg ggccgacggc
      661 catgaccttg cctttctggg gcttctcctt ggcggtgtcg gggatgaaga gaccggaagc
      721 ggtcttctcc tcggcgggct ggggcttgac cacgacacgg tcgcccaggg gcttgattga
      781 ggacatgggc tggtacctcg ctgatgagat ggttggaagg ggaaaccggc gactggcact
      841 cgagcgggaa gagtgctaac gccaggtcaa aaaaatttcg gtgttggcac tctcactggg
      901 agagtgccac atctacctcg tggccgggga caggttccgg tcggcaggcc cagcgcggcg
      961 gcgcggctgc aaaattcgtg tgaaccggcg gggtccagcg gcgtcgaagc gcagcgggaa
     1021 ccggagtcag gatggaagcg gacaggcaaa gggagcgcag ggcgagacga atcgcgttat
     1081 cggtcgggat cctgctcgtg gtgctggtca ccgcgccccc gtacacgggc gacggtgtcc
     1141 gcgcggtcct catggccgcg ttctccggag tgtgccacca gctccctgcc cgctcgcccc
     1201 acgtggacgg cgtgccgctg gccgtctgtt tccgctgcta cgggatctac tgggggatcc
     1261 tgctcgcggc cctgctctac ccggtgctcc ggcgccgact ggggtcgatg gagcgcaccg
     1321 cacccctcgt gctggtcgga tcgctcgtgc cggcgggcgt ggactggctg ctggagctcg
     1381 tcgggctcgt gccgggatca caggccgtcc gcgtggtgac gggcgccgtg ttcggcctgg
     1441 cggcgggcta cttcctggta tccggaatcg cacgggcacg cgggcagccg cgggaagcgg
     1501 gggcgtagga cttcgtccgc tgtcaatttc ccccggtgtc cgctataatc cggcgtccgt
     1561 gatccggacg cgatccgtca ccatcccgat cccgccatgc ccagcaagca gcagtccatc
     1621 gttctcggcg gcgtgctcgt cggcctcctg agcacgtcct atctaggcct catcaacctg
     1681 ctctgctgcc tcggggtgat catcggtgcc ctcgtggccg tgtggcacta cacgtcgcag
     1741 aacgagctga cgctgtcggc cggacagggc gcgggactgg gcgcgctcgc ggccctgctg
     1801 ggctgggcca tcgcgttcgt gctgaactac gtgctcatca aggtgggcgt gcgccacgac
     1861 ctcgtgatga cgcagttcat cctggacagg ttcggcggat ccatgccgcc cgagcagtac
     1921 gaccagatgc tggagcagat gacgcaggag atgacgttcg cgaagtactt cgtgaacagc
     1981 ctcatcggcc tcgccatttc ggctctcttc ggagcgatcg gcggcgccat cggggctgcg
     2041 gtgttcaaga agggtcccgt ccaggatccc ttcgccggag tctgaaccgg ctcagcggat
     2101 ccggcgctgc cgcgagcgcg gcgggccgag gacctccttc aggatgaagg cgtcgcgcag
     2161 ctgccggcgc gttgcgagca ggcgctcggt cggcttcggt tcgggtgcct tcgggaccgc
     2221 ccgggcagcc ttcgccgccg gtcgcgcgtg cgtgtggccc tggagcgggt cgcggaacgt
     2281 gggcaggccc cgctggaaag actcctcgac ctcgatccgt ccggtttcca cgggccggaa
     2341 cgtgtcccgg ggcatcggcg cggccacggg tgtcgggagt ggccgggcgg cgggctcgag
     2401 ccggttgcgg gggacggcct ccggcgcggg ccgcgtggag acttcgggga cgggcggtgc
     2461 gggagggggc tccatgccca gggcccgccg gatttcggcc agcgcgtcgt ccaggctggg
     2521 ctcggcctcg atcgcctccg gctgcggacg tgccggccgg ggcttcttct gggtgggctg
     2581 cttgcgcgag gacagctggc gaagcaggta gaaggccacc agggcgacga cgaagaggag
     2641 gtcggtgtcc acggcggtcc gggtcgggtt cgaggcaaga taaggctccg cggacagggt
     2701 tctcagccgt ccaccccgga cgccagccgc tcgatgagcc tgcggttggc ccgcgggaac
     2761 gcgtagtccg cgagttcctc ccgcgcgacc caggcgaacg cccggccatc ggccggcgtg
     2821 ggctcgccgt ccctgacggt gcacggaaag gcgtgcaagg tcacgcggaa atgggagtag
     2881 gcgtgggcga tctccgccat ccggtcgccg acgtcgacct cgatgccgag ctcctcgcgc
     2941 agctcgcgcc gacaagcgta ctcggggcgc tcgccgggct cgcactttcc gccggggaat
     3001 tcccagagcc cgcccagcat ggcctcctcc ggcctgcgct ggatgagcag ccgcccgtcc
     3061 gcccgccgga cgacgccgac ggcgacatcg aaatgcggca cgggcggacg ccgcctgcgc
     3121 agggggtagg ccgtcggatc gcccgagcgg aagccggcgc agttcgagcg gacgggacat
     3181 gcgtcgcacg acggggtgcg cggcgtgcac accgtggcgc cgag
//
LOCUS       DBNHQZ010000007         4016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3523764, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000007 DBNHQZ010000000
VERSION     DBNHQZ010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3523764"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..954
                     /gene="lipA"
                     /locus_tag="AC16YU_00215"
     CDS             40..954
                     /gene="lipA"
                     /locus_tag="AC16YU_00215"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR6063673.1"
                     /translation="MRPEPSGDGDVLELPVLQPAGAPNERGQRPDWLRVKLPYGDNYK
                     RIVDLVDDFRLHTVCQSARCPNMGECWTSGTATFMILGNVCTRSCGFCAVMTGRPDEG
                     LDYDEPRRVAEAVRIMGVRHAVVTSVNRDERRDGGAPVFAETIRLIREQSPGCTVEVL
                     IPDFRGLWDALQVVLDQRPDILNHNVETVPRLYRRVRPQAVYGRSLELLRRAKDQGLR
                     TKSGIMVGLGETDDEIVALMDDFVVHGVDVMTIGQYLQPTRMHLPVERFVEPATFRWY
                     KEVGETRGIGHVESGPLVRSSYHAERHV"
     gene            964..1743
                     /locus_tag="AC16YU_00220"
     CDS             964..1743
                     /locus_tag="AC16YU_00220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6063674.1"
                     /translation="MFDWSSERRRLSDAVSRLDRQTVTVLCAAALLVILQMQIGDRDV
                     FREHFGAAFEQPWRGLLEWGWWFGMQGVLGFVVPVFLLRRVFHRTADEAGLGRGDWSF
                     AVRVAVLYLPLVAIGTWFLSDGDAFLARYPHLKSAKTDWSVFLVYEAFFLAYWFGWEY
                     LWRGFVLFGTRHTLGLYAIFVQAVPFAILHFNKPMAEALLSLVGGVALGALVWRARAF
                     WIAVPIHAAQMMILDLWCSLRTRTGVRGVGLDALLDLLRGL"
     gene            complement(1839..2750)
                     /locus_tag="AC16YU_00225"
     CDS             complement(1839..2750)
                     /locus_tag="AC16YU_00225"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6063675.1"
                     /translation="MSRAIPILALLWAFSVPASAQSGLAFLRIGPDAAGLALGDAGAA
                     VADGPFVAERNPAGLADAAGTAFGLSHHVWIADVRTYAAAASFRAGERGGIGLFVRAT
                     GSGNLEARQFPGDPDGFFDAQFLVAGIAYGRSFGPVHAGLSVKYLSEKIFAVSADGVA
                     ADFGVRVFPVRGLRLAAAYLHAGSMDELEQRRTRLPRTLRGAVAVYPFRVLAMLDGTV
                     LAEAFLTAEVSRNLVDEVTQWHIGASGHVLDTVQARIGYVANDDLRGVSFGAGVGVAR
                     FQVDYALLPFDQGFDGPGHVFTMAYRL"
     gene            complement(2767..3822)
                     /locus_tag="AC16YU_00230"
     CDS             complement(2767..3822)
                     /locus_tag="AC16YU_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR6063676.1"
                     /translation="MTRTPTQRIRPAAAPAALLMAAVLAAAAAALPASGQDAGAFARN
                     GFGPRGVAMGNALGAVAGASPWYNPALAPFTAVQQLDVSAATLSFDRTAQTVQFATPH
                     LRAGFAVGILHAGVSDIDGRDNAGFHTGMQSVDEFAGYLAFGLRVGSRASAGLALQAF
                     RSDLYDGLDAASTVGIDLGFTLAATDDIRFGLVLDDLLARYSWDSSSLYTEGGRASRD
                     DFPRRVRLAAAVRQLEGRLLISAEYEAGFSSIETASRRVETVGGTPLEFLDLRRITVR
                     DGQLRLGVQGRPVDVLTVRAGIDRIGSGSVRPAAGIELDQEIGNLGARFTYTFAREPY
                     GIGSAHYVGLGLIFDRR"
     gene            complement(3819..>4016)
                     /locus_tag="AC16YU_00235"
     CDS             complement(3819..>4016)
                     /locus_tag="AC16YU_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR6063677.1"
                     /translation="TLRIRVFDSAMNLVRELAESRQGGEGEVTWDGRDERGGRLANGV
                     YFYELSGPGDLGARGKILVIE"
BASE COUNT          585 a         1450 c         1399 g          582 t
ORIGIN      
        1 ccccgcccga accgccccgg tgacgtcacg ctccgcacgg tgcgtccgga gccgtcgggc
       61 gacggggacg tcctggagct gcccgtcctg cagcccgccg gcgcgccgaa cgagcgcggt
      121 caacggccgg actggctgcg cgtcaagctg ccctacgggg acaactacaa gcgcatcgtc
      181 gacctcgtcg acgatttccg gctgcacacg gtatgccaga gcgcccgctg cccgaacatg
      241 ggcgaatgct ggacctccgg cacggccacg ttcatgatcc tggggaacgt gtgcacgcgc
      301 tcgtgcggct tctgcgcggt catgaccggc cgtccggacg aggggctcga ctacgacgag
      361 ccgcgccgcg tggccgaggc cgtgcgcatc atgggcgtgc gccatgccgt ggtcaccagc
      421 gtgaaccgcg acgagcgcag ggacggcggc gctcccgtct tcgccgagac catccggctc
      481 atccgcgagc agtcgccggg ctgcacggtc gaggtgctca ttccggactt ccggggcctg
      541 tgggacgcgc tgcaggtcgt gctcgaccag cgtcccgaca tcctgaacca caacgtcgag
      601 accgtccccc gcctttaccg cagggtccgg ccgcaggccg tctacgggcg ctcgctcgag
      661 ctgctgcgcc gcgccaagga ccagggcctt cgcacgaaga gcggcataat ggtgggcctc
      721 ggcgagacgg acgacgagat cgtcgccctc atggacgact tcgtggtcca cggcgtggac
      781 gtgatgacga tcggccagta cctgcagccg acccgcatgc acctgcccgt cgagcgcttc
      841 gtggagccgg ccaccttccg gtggtacaag gaggtgggcg agacccgcgg catcggccac
      901 gtggagagcg gcccgctcgt ccggtcgtcc taccacgcgg aacggcacgt ctgaggccgg
      961 tcgatgttcg actggagctc cgagcggcgg cggctctccg atgccgtgtc ccggctggac
     1021 cggcagaccg tcaccgtcct gtgcgcggcc gcgctcctcg tgatcctgca gatgcagatc
     1081 ggcgaccggg acgtgttccg ggagcatttc ggcgcggctt tcgagcagcc ctggcgcggc
     1141 ctcctcgagt ggggctggtg gttcggcatg cagggcgtgc tcggcttcgt ggtccccgtg
     1201 ttcctgctcc ggcgggtgtt ccaccgcacg gccgacgagg cggggctcgg gcgcggcgac
     1261 tggagcttcg ccgtccgcgt cgccgtcctg tacctgccgc tggtcgccat cggcacctgg
     1321 ttcctctcgg acggagatgc cttcctcgca cgctaccccc acctgaaatc cgcgaagacg
     1381 gactggagcg tcttcctcgt ctacgaggcg ttcttcctcg cctactggtt cgggtgggag
     1441 tacctgtggc gaggcttcgt gctcttcggc acccggcaca ccctgggcct gtacgcgatc
     1501 ttcgtgcagg ccgtgccctt cgccatcctc catttcaaca agcccatggc cgaggcgctg
     1561 ctctcgctcg tgggcggcgt ggcgctcggc gccctggtgt ggcgcgcccg cgcgttctgg
     1621 atcgccgtcc ccatccacgc ggcccagatg atgatcctgg acctctggtg ctcgctccgg
     1681 acccggaccg gcgtccgggg cgtgggcctc gacgccctcc tggacctcct gcgcgggctc
     1741 tgatatctcc gccccggtac gtcgcagtga ggggatgcgc cgcccaacgc cgagatgccg
     1801 gattacccgt cggcccggat gccggattac ccgtcggcct acaggcgata ggccatcgtg
     1861 aacacgtgcc ccggcccatc aaacccctgg tcgaacggca gcagcgcata gtccacctgg
     1921 aaccgggcca cgccgactcc ggccccgaag gagactcccc gcaggtcgtc gttagcgacg
     1981 tagccgattc tcgcctgcac ggtgtccagc acgtggcccg acgcgccgat gtgccactgg
     2041 gtgacctcgt cgaccaggtt ccggctgacc tcggcggtca ggaaggcctc ggcgagcacg
     2101 gtcccgtcga gcatcgccag gacgcggaag ggatagacgg ccacggctcc ccgcaaggtt
     2161 cgcggcaggc gtgtgcgccg ctgctcgagc tcgtccatgc tgccggcgtg caggtaggcg
     2221 gcggcgagcc gcagcccgcg gaccggaaaa acgcgcaccc cgaagtcggc cgcgacgccg
     2281 tcggccgaga cggcgaagat cttctcggag agatacttga cggagagccc ggcatgcacg
     2341 ggcccgaacg agcggccgta cgcgatgccg gcaacgagga actgggcgtc gaagaacccg
     2401 tccggatcgc ccgggaactg ccgggcctcc aggttgcccg aaccggtcgc gcggacgaag
     2461 aggccgatgc ccccccgctc gccggcgcgg aacgacgcgg cggcggcgta cgtgcgcacg
     2521 tcggcgatcc acacgtggtg gctgagcccg aaggccgtcc ccgcggcgtc cgcgagcccg
     2581 gcgggatttc gctcggcgac gaagggaccg tcggcgacgg ccgcgccggc atccccgagc
     2641 gcgagccccg cggcgtccgg cccgatgcga aggaaggcca ggccgctctg agcggaagca
     2701 ggcaccgaga aggcccacag cagggccagg atgggaatcg cgcgactcat ggtcctcaat
     2761 tggtcctcat cgcctgtcga agatcagccc caggcccacg tagtgcgccg atccgatgcc
     2821 gtatggctcg cgtgcgaacg tgtaggtgaa ccgggcgccc aggtttccga tctcctggtc
     2881 gagctcgatg cccgcggccg gccgcacgct cccgctcccg atccggtcga tcccggcgcg
     2941 gaccgtgagc acgtccacgg gacggccttg cactcccagg cgcagctgtc cgtcccgcac
     3001 cgtgatgcgt cggagatcca ggaactcgag cggcgttccg ccgacggtct ccacgcggcg
     3061 cgaagccgtc tcgatgctcg agaagcccgc ctcgtactcc gccgagatga gcaggcgccc
     3121 ctcgagctgc cgcacggcgg ccgccaggcg gacgcggcgt gggaagtcgt cccggcttgc
     3181 ccggcccccc tccgtgtaca gcgaggaaga gtcccagctg tacctcgcga gcaggtcgtc
     3241 gaggaccagc ccgaagcgga tgtcgtcggt ggccgccagc gtgaagccca ggtcgatgcc
     3301 cacggtggac gccgcgtcga gcccgtcata caggtcggat cggaaggcct ggagcgccag
     3361 ccccgccgag gcccgcgagc cgacccggag gccgaaggcc aggtatccgg cgaactcgtc
     3421 caccgactgc atgcccgtgt ggaagccggc attgtcgcgc ccgtcgatgt cggatacgcc
     3481 ggcgtgcagg atgccgaccg cgaacccggc tctcaggtgg ggagtcgcga actgcaccgt
     3541 ctgggccgtc cggtcgaacg agagcgtcgc cgccgaaacg tccagctgct gcacggccgt
     3601 gaagggcgcg agggcgggat tgtaccacgg gctcgccccc gcgacggcac ccagggcgtt
     3661 gcccatcgcc acgccgcgcg gaccgaatcc gttccgcgcg aatgcgcccg cgtcctgtcc
     3721 cgaggccgga agcgcggccg ccgcggcggc gagaacggcc gccatgagga gcgcggccgg
     3781 tgcggcggca gggcggatcc tctgggtcgg agtgcgggtc attcgatcac caggatcttg
     3841 ccccgcgctc cgaggtcgcc cggacccgag agctcgtaga agtacacgcc gttggcgagg
     3901 cgtccgccgc gctcgtcccg gccgtcccat gtgacctcgc cttccccacc ctgccggctc
     3961 tccgcgagct cacggacgag gttcatggcg gaatcgaaca cgcggatgcg gagggt
//
LOCUS       DBNHQZ010000008         4004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_814200, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000008 DBNHQZ010000000
VERSION     DBNHQZ010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_814200"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC16YU_00240"
     CDS             complement(<1..868)
                     /locus_tag="AC16YU_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063678.1"
                     /translation="MRSLAGRTLAFVLLFTAPTISLYAQPTATDWHARAALLISDLRP
                     VSAAQAEYVLLGVQRQNRFMDAWTTISETTHAYDGARRLESTDHEWNGFELQPTYRTT
                     YAPDGQVQAVDLWDSGADDFVPETRFSYEYQYDLDSGQTVVSVATTQLWNGVGWADHE
                     RTTYAVEPVPSGGLRVTGGVTELGSPSGWAPYTDFVVEEVAGDVVHIIRFWSGSGWTN
                     QARYTYPSTTIQDLDARIRQLALEYRDTAGLNLGLLSLPDMEVALWSDAQQAWLPNNR
                     RTTEAFHFGTGKP"
     gene            complement(1059..1132)
                     /locus_tag="AC16YU_00245"
     tRNA            complement(1059..1132)
                     /locus_tag="AC16YU_00245"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Asp,seq:gtc)
     gene            1261..1638
                     /locus_tag="AC16YU_00250"
     CDS             1261..1638
                     /locus_tag="AC16YU_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6063679.1"
                     /translation="MHSIHTDAAPRPAGHYAQAVVHGGLVFVSGQLPVDPATREPVLG
                     PVEEQTRRALANVEAILREAGSDLSRVLRTTVYVADIALWGRVNAAYAEVFGDHRPAR
                     AVVPVPELHHGVLVEVEAVAFVA"
     gene            1767..>4004
                     /locus_tag="AC16YU_00255"
     CDS             1767..>4004
                     /locus_tag="AC16YU_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6063680.1"
                     /translation="MIGRTVGQFRIVEKLGQGGMGVVYKAIDTRLNRTVALKFLPAHL
                     SADAKAKARFIQEAQAASSLDHPSICTIFDIGETEEGEVFIAMAFYEGQTLKYRLSGE
                     ALSPEDTLSIARQLARALARAHEAGIVHRDVKPANIMWTDRGELKLLDFGLAKFAGGA
                     ELTMAGTTLGTAAYMSPEQVRGDRVGPPSDVWAFGALVYELLTGKRAFAADYDQALLY
                     GILNEDPEPVRSLRPAVPEPLSDLVSACLRKEPERRPGFDEILEVLGDGSSAEREVRG
                     SGTMSIRASAPPARVAPRSRRLVPIGVAAGIVVAVALLWFAPRPGGDPGRTDGAPGAT
                     THQSVAVLPFRTTAGAAEDARAFADGMHDDVLTRLGQISDLRVISRTSMLQYEGTSKS
                     IREIARELDVATVLEGSVQRSGNRVRVNVQLIDATTDGQLWAESYDEDLTPENVFGIQ
                     ADLARQIAAAMQATLTPEEEARIAQCDSTSLEVYDLYTQAVYLLRNSQRGDETAIRRA
                     LSILEGATRQDPGFGVAYARMARTYYLSVQFGIFTREEARTLADPLIEKALALDPDNI
                     ETQLAKGVAAEDRLDFAEAEAAYRRAIELAPGAADGYRAVARVLYLMGRPEEAMRLAR
                     LAVQMDPLDIEVRETLADALFFTNDFTGSVAESRRILAMEPNRTYSLYNMGYGLMVLG
                     QSDEALAAFERAVEIDSGDSWNRLGYAWALARVGRVEESLAMMNSVSDTPDSKKEKAI
                     IYGELG"
BASE COUNT          653 a         1317 c         1414 g          620 t
ORIGIN      
        1 ccggcttgcc cgtgccgaag tggaaggctt cggtcgtccg ccggttgttc ggaagccagg
       61 cctgctgggc atcggaccag agcgcgactt ccatgtccgg cagcgagagc aggcccaggt
      121 tcaggcccgc cgtgtcccgg tattccaggg caagctggcg gatcctggcg tccaggtcct
      181 ggatcgtcgt cgacgggtac gtgtaccggg cctggttcgt ccagccggac ccggaccaga
      241 accggatgat gtgcacgacg tcgccggcca cttcctcgac gacgaaatcg gtgtagggcg
      301 cccagccgga cggggatccg agctcggtga cgccccctgt cacgcggagc cctccggacg
      361 ggacgggctc gacggcatag gtcgttcgct cgtggtcggc ccatccgacg ccgttccaga
      421 gctgggtcgt ggcgacggac acgacggtct gcccggagtc gaggtcgtac tggtactcgt
      481 agctgaaccg ggtttccggc acgaagtcgt ccgcgccgga atcccacagg tctacggctt
      541 gcacctggcc gtccggtgcg taggtcgtgc ggtaggtcgg ctgcagctcg aacccgttcc
      601 actcgtggtc ggtgctctcg agccggcgag cgccgtcata cgcatgcgtg gtctcggaga
      661 tcgtggtcca tgcgtccatg aaccggttct gtcgctgaac gcccagcagc acgtactccg
      721 cctgggcggc ggaaacgggc cggaggtcgc tgatcagcag ggcggcgcgc gcgtgccagt
      781 cggtcgcggt cggctgggcg tacagggaaa tggtcggtgc cgtgaaaagg aggacgaacg
      841 cgagcgtccg tccggcgaga gagcgcatct tggtctgggt gctccgcggt tgggaacggg
      901 ttgtcagggt cgtgaatggg gggcatgagc ggcgatattc ggggaatcca gtgcgcggtt
      961 gcggggcccg ttgaaattca caatccggca cgtggccccg ctccgaacga aaaaccccgc
     1021 agtccgtcgg caggcgacga gctgcggggc cataagcgcg gtccggacgg gactcgaacc
     1081 cgcgacctcc gacgtgacag gccggcgttc taaccaactg aactaccgga ccgaaatgcg
     1141 ctcaagacag gtaatataga gcgaccgggc aggcctccac aaacggggcg attggaaatt
     1201 ttgtaaagcg gcgctgccgg gatccgtcgt agactcgggc tcccatccca cccgaagccc
     1261 atgcattcga ttcacaccga cgccgctccg cgcccggccg gacactatgc tcaggccgtg
     1321 gtgcacggcg gcctggtctt cgtatcgggg cagctgcccg tcgacccggc aacgcgcgaa
     1381 ccggtcctcg gcccggtcga agagcagacc cgccgcgcac tcgcgaacgt cgaggcgatc
     1441 ctccgcgagg ccggctcgga tctgtcccgg gtgctccgga caaccgtgta cgtggcggac
     1501 atcgcgctct ggggccgggt gaatgcggcg tacgcggagg tattcggcga tcaccggccg
     1561 gcccgagccg tcgtgccggt cccggagctg catcacggcg tgctggtaga ggtcgaagcg
     1621 gtagcgttcg tggcgtgatg ggccgcatcg gccgctattt cccggaggca cgctgaccga
     1681 tatattcggg cgcacgcgct acccccgggt cgcgggcggc cggaaggaaa gcccgggaat
     1741 ctccgttgac gcgtaacagc agttccatga taggtcgcac cgtagggcag ttcaggatcg
     1801 tcgaaaagct cggccaggga gggatgggtg tcgtctacaa ggcgatcgat acccggctca
     1861 atcggaccgt cgcgctcaag tttctcccgg cgcatctgag cgcggacgcc aaggcgaagg
     1921 cacggttcat ccaggaggcc caggcggcct ccagcctgga tcacccgagc atctgcacga
     1981 tcttcgacat cggcgagacg gaggaggggg aagtcttcat cgccatggcg ttctacgagg
     2041 ggcagaccct caagtaccgt ctctcgggcg aggccctttc gcccgaggac acgctgtcga
     2101 ttgcccgcca gctcgcgcgg gcgctcgcca gggcacacga ggccggcatc gtccaccggg
     2161 acgtgaagcc cgccaacatc atgtggacgg atcgcggaga gctcaagctg ctcgatttcg
     2221 gcctcgcgaa gttcgcgggc ggcgccgaac tgacgatggc gggaaccacc ttgggcacgg
     2281 cggcctacat gagccccgag caggtgcgag gcgaccgcgt cgggcccccg tcggacgtat
     2341 gggccttcgg cgccctcgtg tacgagctcc tgacgggaaa gcgggccttc gccgcggact
     2401 acgaccaggc actcctgtac ggaatcctca acgaggaccc cgagcccgtc cggtcgcttc
     2461 gacccgccgt gcccgagccg ctgtcggatc tcgtctcggc ctgcctgcgg aaggagccgg
     2521 agcggcgacc gggattcgac gagatactcg aggtcctcgg cgacggatcg tccgccgagc
     2581 gcgaggtccg ggggtcgggg acgatgtcca tacgggcttc cgcgccaccg gcgcgcgtcg
     2641 ccccgcgcag tcgccggctc gttccgatcg gcgtcgcggc gggcatcgtg gtcgccgtcg
     2701 ccctcctctg gtttgcgccg cggccgggcg gggatccggg aaggaccgac ggggcgccgg
     2761 gggcgaccac gcaccagtcg gtcgcagtac ttccgttccg gacgaccgcc ggtgcggcgg
     2821 aggacgcccg tgcgttcgcg gacggcatgc acgacgacgt actgacgcgc ctgggccaga
     2881 tttcggacct gcgcgtgatt tcgcgcacct cgatgctgca gtacgagggc acctcgaaga
     2941 gcattcgcga gatcgcccgg gagctcgatg tcgcgaccgt gctcgagggc tccgtccagc
     3001 gatccgggaa ccgggtccgc gtcaacgtgc agctgatcga cgcgacgacc gacggccagc
     3061 tctgggccga atcctacgac gaggacctca cgccggagaa tgtcttcggc atccaggccg
     3121 acctggcccg gcagatcgcc gccgccatgc aggccacgct cacgcccgag gaggaagccc
     3181 ggatcgcgca gtgtgacagc acgagcctcg aggtgtacga cctgtatacg caggcggtct
     3241 acctgctgcg gaacagccag cgcggcgacg agacggcgat acgtcgcgcc ctgtccatcc
     3301 tcgagggggc gacccggcag gatcccggtt tcggcgtcgc ctatgcgcgc atggcccgga
     3361 cgtactacct gtcggtgcag ttcggcatct tcacgaggga agaggcccgc acgctcgccg
     3421 atcccctcat cgagaaggcc cttgcgctcg atccggacaa catcgagacc cagctcgcga
     3481 agggcgtcgc ggccgaggac cggctcgatt tcgcggaagc cgaggcggcg taccgtcgtg
     3541 ccatcgagct ggcgccgggc gccgctgacg gctaccgggc cgtggcccgg gtcctgtacc
     3601 tgatggggcg tcctgaagag gcgatgcggc tggcccggct ggccgtgcag atggaccccc
     3661 tggacatcga ggtgcgggag acgctcgccg acgcgctctt cttcaccaac gatttcaccg
     3721 gaagcgtggc cgagtcccgc cgcatcctgg cgatggagcc gaaccggacc tactcgctct
     3781 acaacatggg atacggcctg atggtgctcg ggcagtccga cgaggccctg gcggcgttcg
     3841 agcgggcggt cgagatcgac agcggggatt cgtggaacag gctgggctat gcctgggccc
     3901 tggcgcgggt cggccgcgtg gaggagtccc tcgccatgat gaactccgtg tccgacaccc
     3961 ccgactccaa gaaggagaag gccatcatct acggcgagct gggg
//
LOCUS       DBNHQZ010000009         3687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3659366, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000009 DBNHQZ010000000
VERSION     DBNHQZ010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3659366"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16YU_00260"
     CDS             complement(<1..180)
                     /locus_tag="AC16YU_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR6063681.1"
                     /translation="MRPSFLALLVIAAVAASCSPSEPTAPARTTLVAFGSCAQQDLPQ
                     PIWNAVLETGPDAFLF"
     gene            complement(294..1178)
                     /gene="porD"
                     /locus_tag="AC16YU_00265"
     CDS             complement(294..1178)
                     /gene="porD"
                     /locus_tag="AC16YU_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorD"
                     /protein_id="HIR6063682.1"
                     /translation="MRVFLLSILLAAVPLSARAQEFNCSVSVNYRNLTGSDYTYLDEL
                     KQRVSEYINLQRWTEDRYEEHERIECTLQIVFLEAVSLTRFKTRLIVATRRPIYGTTQ
                     QTTVLQLSDEDWTFDYAQGTPLVYDPERYHPITSVLNFYALIMLGFDYDTFSPEGGSP
                     FFDRARRVSDIAQSSGASGWSTLGADRSRTDLITQIMDPRFKPLRASYYDYHFGCLDR
                     FVADTETARDVMLELVKGLQTLAMNQSRSYYLDQFFATKYQEIAAVFQSSRLASQAFD
                     VLAQVDPAHMSEYNRMME"
     gene            1276..2055
                     /locus_tag="AC16YU_00270"
     CDS             1276..2055
                     /locus_tag="AC16YU_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063683.1"
                     /translation="MRLGSCLLLLLVPLAAAGQSAWLDAASRSMGGAGVALGSGGPYP
                     LNPAALPEYGAVSIGSTRLYGLPDLTPLRIGIGLGRGAASAAFAVTGLKLEGYSDLRM
                     SLAASTRVGGPRMGIRVGLARREAGPYPSTHALLVVAGLRHGLTPRWSAALLAGRALG
                     RGSPRGEAPVLGVGAAWSDERLTLPVEIRRAGAAPPSFHAGMQVRPVPALALRVGMST
                     APDLVAVGIGLALGRMGIDMAVDFHPDLGHSLSLDLRMTGR"
     gene            2052..>3687
                     /locus_tag="AC16YU_00275"
     CDS             2052..>3687
                     /locus_tag="AC16YU_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063684.1"
                     /translation="MTTRTPILPVLALLLCAPERGLAQATDSLDAAWDADVLPPESVM
                     EHSTDDARPGRGGALLAWRSGWSRSHSTAGGRHALRFSRTGGSFELRLAGDHDPGERL
                     VTRTAPRLFDHVVVSARWAVSRRLQWIAGSFRRSFFHGLTAGSERFGAASSDPGGSTR
                     TGTGLRAWAAASEDLYLNGTALQADLAPGLTWSAWASRRLLDAAVDSSGPAPTARWVL
                     GGRHVTALERTRAGAVSYREVGVAVRWILGAVSIGGVLLHEAFGLRFCPGACDGPASH
                     RRRGHYGSVYVGLSARAGSLGVEARPTSGGWDVVAAGTATVRPAGSLRVLARRYGSSG
                     GPILASPPSAGSGSVPDEHGLLVHWRMRMAPGVILDLASDHARVPFGSPSLSGRAARL
                     DQRVSLEVRRASWEGVATLRRTVRTEPGSGVAEPDADRIARRRVRLKVSLPVGRSVVI
                     AAMADGADAGRSDAPGTAAMGISVDWSPNASWRLRAGSTEYAAASGTPVVTYEPDTPL
                     AYPLRVLHGSGRRAWVLVEWRGRGTSVSGSAGRGLRF"
BASE COUNT          487 a         1258 c         1355 g          587 t
ORIGIN      
        1 gaaaaggaac gcgtcgggcc ccgtctcgag cacggcgttc cagatgggct gcgggaggtc
       61 ctgctgggcg caggagccga aggcgacgag ggtcgtccgg gccggcgcgg tcggctccga
      121 cggggagcac gacgcggcga ccgccgcgat gacgaggagg gcgaggaagc tgggtcgcat
      181 gggagcctgc tgcgatgggt gcggaatgtc acaagatccc gcaaggctcg ctcctgcggg
      241 accggacatt tccggcaacc ggaagccggg attcccctac cgtcccatcc gtcttactcc
      301 atcatccggt tgtactccga catgtgggcc ggatccacct gggccagcac gtcgaaggcc
      361 tgcgacgcca ggcgggagct ctggaagact gcggcgatct cctggtactt cgtggcgaag
      421 aactggtcca ggtagtacga gcgcgactgg ttcatggcca gggtctggag acccttcacc
      481 agctccagca tcacgtcccg ggcagtctcg gtgtccgcga cgaaccggtc caggcagccg
      541 aagtggtagt cgtagtacga ggcccgcagc ggcttgaagc gcggatccat gatctgcgtg
      601 atcaggtcgg tgcgactccg gtcggcgccg agggtcgacc agccggacgc gccgctcgac
      661 tgcgcgatgt ccgacacccg ccgggcgcga tcgaagaagg gcgagccccc ttccgggctg
      721 aacgtgtcgt agtcgaatcc gagcatgatc agcgcgtaga agttcagcac cgacgtgatc
      781 gggtgatacc gctccggatc gtacacgagc ggcgttccct gtgcgtaatc gaacgtccag
      841 tcctcgtccg acagctggag gacggtggtc tgctgcgtcg taccgtagat cgggcgccgc
      901 gtggcgacga tgagccgggt cttgaaccgg gtgagcgaca ccgcctcgag gaagacgatc
      961 tggagggtac actcgatccg ctcgtgctcc tcgtagcggt cctcggtcca ccgctgcaga
     1021 ttgatgtact cgctcacccg ctgcttgagc tcgtccaggt aggtatagtc cgaccccgtc
     1081 aggttccggt aattgaccga aaccgagcag ttgaactcct gagcgcgggc cgagagcggg
     1141 accgcggcga gcaggatcga gaggagaaag actctcatgg ggttcaggca ccggtaggcg
     1201 cggttcatac aggcaggatg tgctccggtt tccgtcgccg ggttatgccg gagggccttc
     1261 acggatcagt ggaccatgcg cctcggatcc tgcctgctac ttctcctcgt tccgctcgcg
     1321 gcggccgggc agtcggcctg gctcgacgcc gcctcgcgct cgatgggcgg ggccggagtg
     1381 gccctcggat cgggcggtcc gtatccactc aatccggccg cgcttccgga atacggggcg
     1441 gtgtcgatcg gcagcacgcg gctgtacggc ctccccgacc tcacgcccct ccggatcggg
     1501 atcggcctgg gtcggggcgc ggcgagcgcg gccttcgcgg tcaccggtct gaagctcgag
     1561 ggctactccg acctgcgtat gtccctcgcg gcgtcgaccc gagtcggcgg accgcggatg
     1621 ggcatccgcg tcggtctcgc gcgccgcgag gccggaccgt acccgtccac ccacgcgctc
     1681 ctggtcgtgg ccgggctccg gcacggtctc acgccccggt ggtcggcggc tctcctggcg
     1741 ggtcgggcgc tcggccgggg ctccccgcgc ggcgaagccc ctgtcctggg cgtcggggcg
     1801 gcatggagcg acgaacgcct cacgctcccg gtggaaatca ggcgggccgg cgccgctccg
     1861 ccatcgtttc atgccggcat gcaggtccgc cccgtcccgg cccttgcgct tcgcgtcggc
     1921 atgtcgacgg cgcccgacct cgtggccgtg ggcatcggcc tcgcgctcgg gcggatgggc
     1981 atcgacatgg ccgtcgattt tcacccggac ctcggccatt ccctgtccct cgatctgcgg
     2041 atgacgggcc gatgaccacg cgaacgccga tcctgcccgt cctggccctg ctgctatgcg
     2101 cgccggagcg gggcctcgcg caggcaaccg acagcctgga cgcggcctgg gacgccgacg
     2161 tcctgccccc cgagtcggtc atggagcact ccacggacga cgcgcgcccg ggtcgcggcg
     2221 gcgccctgct cgcctggcgg tcgggctgga gccgatcgca ctcgacggcc gggggccggc
     2281 acgcgctccg cttctcacgc acgggcgggt ccttcgaact ccgcctcgcc ggggaccacg
     2341 atccgggcga gcgtctggtg acccgtaccg cgccccgcct cttcgaccac gtggtcgtct
     2401 cggcgcgttg ggcggtgtcg cgccggttgc agtggatcgc gggcagcttc cgccggagct
     2461 tctttcacgg gctgacggcg ggcagcgaac ggttcggggc ggcctcctcc gatcccggcg
     2521 ggagcacccg cacgggtacc ggcctgaggg cgtgggccgc ggcgagcgag gacctgtacc
     2581 tgaacggaac ggccctgcag gcggatctcg cgccgggcct cacctggtcc gcatgggctt
     2641 cacgtcgctt gctcgacgcc gcagtcgatt cgtccggccc ggcacccacc gcaagatggg
     2701 tcctgggggg acggcacgtg accgccctcg agcgcacgag agcgggcgcc gtcagctacc
     2761 gcgaggtcgg cgtggcggtc cggtggattc tcggagccgt atcgatcggt ggcgtgctcc
     2821 tgcacgaggc gttcggcctg cggttctgtc cgggcgcgtg tgacgggccc gcaagccatc
     2881 gccgaagggg gcattatgga tccgtatacg taggcctttc cgcccgggcc ggcagcctcg
     2941 gcgtcgaagc ccgtccgacg tcgggtggat gggacgtcgt ggccgccggc acggcgacgg
     3001 tgcgtcccgc cggaagcctc cgtgtgctcg ccaggcgata tggatcgtcg gggggaccga
     3061 tcctggcctc gcctccgtcg gccggcagcg gctccgtgcc ggacgagcac ggcctgctcg
     3121 tccactggag gatgcggatg gctcctggcg tgatcctgga cctggcctcg gatcacgccc
     3181 gcgtgccgtt cggatcgccc tccctgtccg ggcgggccgc ccgcctcgac cagcgggtct
     3241 cgctcgaggt gcgccgggcg tcgtgggaag gcgtggccac gttgaggcga accgtgcgca
     3301 cggagccggg ctcgggggtg gcggagcctg acgcggaccg gatcgcgagg cgacgcgtcc
     3361 ggctcaaggt cagcttgccg gtgggccggt ccgttgtgat tgcggcgatg gcggatggcg
     3421 cggatgccgg gcgttcggac gcgccgggca cggccgccat gggcatcagc gtcgactggt
     3481 cgcccaatgc gtcctggcgc ctcagggcgg gatcgacgga gtacgccgcg gcctccggta
     3541 cgccggtggt gacgtacgag cccgacaccc cgctcgccta cccgctccga gtgctccacg
     3601 gatcgggccg aagggcatgg gtgctggtgg agtggcgggg ccgcgggact tcggtcagtg
     3661 gctcggccgg gcgcggcctg cgctttc
//
LOCUS       DBNHQZ010000010         5620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1085595, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000010 DBNHQZ010000000
VERSION     DBNHQZ010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1085595"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="AC16YU_00280"
     CDS             <1..1224
                     /locus_tag="AC16YU_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HIR6063685.1"
                     /translation="EGGGVYLFEQRVRIVDEGDTLTADRVRYDRNARFGRATGSVRLG
                     DGEVVVLAPEGDYDTGAKRADFRAGVTLIDSASTLSSRSGSYWTGERRAEFAGDVRLE
                     QDSLRMAADSLLYLRDRRLAEAWSGVVIERREGEGAGVTWVLGRHAVNDEEAGRSVVR
                     GDVLLLRVAADSTSADSLWIRSGRVDFARTDSLETIVAVDSVRIAGDDFAALADSVEY
                     RIEATRPDAAPARREGRFFGSPMAWFGTSQVSGDSIRVAGRDQELDTLVVQGAARLAL
                     RDSTDAPMQQLRGRRMVGLFERDSLRTLTVMSNAEALYHMTSAERPDAGAVRTSGDRV
                     VLRLEGGEVRDIRIYEGVEGTWYPASLVDDAVSLEGVAWRPDLRPDAEPLRARYLTAR
                     CRIDAAGCRASAEPR"
     gene            1567..2319
                     /gene="lptB"
                     /locus_tag="AC16YU_00285"
     CDS             1567..2319
                     /gene="lptB"
                     /locus_tag="AC16YU_00285"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR6063686.1"
                     /translation="MHPAEDAAVLRAEDLVKRYRKRSVVRAVTLEVRQGEIVGLLGPN
                     GAGKTTTFYMIMGLARPDRGRVYLDDADLTRLPMYERARRGIGYLAQEASIFGNLTVE
                     QNLEAVLEYQGLPKAERAARADALVGEFGLERVRRSKGYMLSGGERRRTEIARALAAR
                     PKFFLLDEPFAGVDPIAVEDIQAIVAGLKARGIGVLITDHNVHETLAITDRAYLLYEG
                     RILKAGTAEALASDPEVRKRYLGEKFTLERYR"
     gene            2385..3701
                     /gene="tyrS"
                     /locus_tag="AC16YU_00290"
     CDS             2385..3701
                     /gene="tyrS"
                     /locus_tag="AC16YU_00290"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6063687.1"
                     /translation="MNPAADPSAVVTPPLADLHADLRWRGMVYDATPGLDEVLRTERL
                     TAYIGFDPTASSLHVGSMLPIMGLARLQRCGHTPIAIVGGGTGLIGDPSGKTAERRLL
                     DAAQVEENLQGIRAQLERFLDFDARGNPARIVNNLDWLGELRLVDFLRDTGKHFTVNY
                     MLAKESVKRRLEAEDGISYTEFTYMMLQAYDYLVLHDRYGCTLQMGGSDQWGNILAGS
                     DLIRRIRGARVHGMVFPLVTNAAGTKFGKTEAGTVWLDPQRTSPYRFYQFWLNTDDRD
                     VVAYLKYFTWLDGAAVDALEESVADRPESREAQRTLAREVTTLVHGEPETRRAERAAA
                     VLFGGDLDELSAHDLLDVFDDVPSADMDRETFAGDGLPLIDLLADSGLTASKGEARRL
                     VRSGGVFVNNRRMTDEQARVGIDSAVDGLLLVLRKGKKNYRLVKIR"
     gene            3722..4654
                     /locus_tag="AC16YU_00295"
     CDS             3722..4654
                     /locus_tag="AC16YU_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012991.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063688.1"
                     /translation="MVIVMRSGAGEAQIHAVIDRLNAFGFDVHRSSGLQRTVLGAIGA
                     KPGFDIRHIASLDGVEAVHRVTEPYGRVLRRMDDPEPRWSIGSRVFGPGRIAVVVSLV
                     DADVHAEAIAAVVRSGADALSLPAPAWSAEAGAAASGAGLPVLIEIAHGAQLPAADVR
                     PAAWRLTGTAMFERDLRHRIAALGAPVIVDRAPGSTIEEWLLAADDLLGAGCTDVALC
                     DSGSRSLDPARSLAPDIAALVFAAVRTRLPVLADPGRAIADPAMALAAACAAVGAGAR
                     GIVLTLRDRRGEPDVAVLERAGERLRAIAAAVGR"
     gene            4714..>5620
                     /gene="prfA"
                     /locus_tag="AC16YU_00300"
     CDS             4714..>5620
                     /gene="prfA"
                     /locus_tag="AC16YU_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6063689.1"
                     /translation="MRVLSRPEMIADRVLNAILDRHREVNDMMADPNVAVNVSRMKEL
                     GREHTALRDVVSGIEKYRAMLRERDDLRSLVESEDDAEFVRMARAELSDLEDRIPEFE
                     EELRLQLLPRDPDDSKDCIVEIRAGTGGDEACLFAGDLYRLYSRFAEGKGWKLDLIDA
                     SEGPQGGFKEIIFGLSGADAFGTMKYESGVHRVQRVPVTESSGRIHTSAATVAVLPEA
                     EEFDVQIGPNDLKIDVYRSSGPGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQHKNKD
                     KAMRVLRSRLYEQELERRKSERSEAR"
BASE COUNT          838 a         1900 c         2062 g          820 t
ORIGIN      
        1 gagggagggg gcgtgtacct gttcgagcag cgggtgcgga tcgtggacga gggggacacg
       61 ctcacggccg atcgcgtccg gtacgaccgc aacgcacggt tcggacgggc gacgggatcc
      121 gtccggctgg gggacggcga ggtggtggtt ctcgccccgg agggcgacta tgacacgggt
      181 gcgaagcggg ccgacttccg tgccggggtg acgctgatcg acagcgcgtc caccctctcg
      241 agccgctccg gttcgtactg gaccggggag cggcgcgcgg aattcgcggg agacgtgcgc
      301 ctggagcagg acagcctgcg catggccgcc gattccctcc tgtaccttcg ggatcgccgg
      361 ctcgccgagg cctggtcggg cgtcgtgatc gagcgacgcg agggagaggg cgcgggcgtg
      421 acatgggtgc tgggccggca tgccgtgaac gacgaggagg ccgggcggag cgtggtccgg
      481 ggcgacgtcc tcctgctccg cgtcgccgcg gattcgacct ccgcggattc gctctggatc
      541 cggtccggcc gcgtggactt cgcccggacc gactccctcg agacgatcgt cgcggtggac
      601 tcggtgcgca tcgcgggcga cgacttcgcc gccctcgccg actccgtcga gtaccggatc
      661 gaggcgaccc ggcccgatgc cgcgccggcg cgccgggagg gccgcttctt cggctcgccg
      721 atggcatggt tcgggacgag ccaggtgtcg ggcgattcga tccgggtcgc cggccgggac
      781 caggagctgg acacgctcgt ggtccagggc gccgcgcgcc tcgccctcag ggattcgacg
      841 gacgcgccga tgcagcagct ccgggggcgt cgcatggtgg gcctcttcga gcgggacagc
      901 ctgcgcaccc tcacggtgat gtccaacgcc gaggcgctct accacatgac ctccgccgag
      961 cggccggacg cgggcgcggt ccggacatcc ggagaccggg tcgtgctccg cctcgaaggc
     1021 ggcgaggtcc gggacatccg catctacgag ggcgtcgagg gcacctggta tcccgcgtcg
     1081 ctcgtcgacg atgccgtgag cctcgagggg gtggcctggc gtccggatct tcggccggac
     1141 gcggaaccgc tccgcgcgcg ctaccttacg gctcgctgca ggatcgacgc cgccggctgc
     1201 cgggcgtcgg ccgagccccg ctagccccgg aatccgccgg aaccgtcgac catgccgatt
     1261 gtcgagatcg agcaggccct cgagcacttc aagcgcggtg acccggcatc ggccgtgccg
     1321 gtgctcaggg aagtcgtgac gctggttccc acctacgtca tggcgcacgt gctcctggcg
     1381 caggcttacg aggccctgca acaggacgag gaggcggcgg actcctggcg ccgggtccag
     1441 cggctgatcc ccatgagccc gcccggtgct gcgctgccct cgctcgagga ttcggacccg
     1501 gatttcgtgc acttcgccga gcgcctgcgc gcggcggact gggccgccgc cgccgccggc
     1561 ccccgcgtgc accctgccga ggatgccgcc gtgctgagag ccgaggacct ggtgaagcgg
     1621 taccgcaagc gttccgtggt gcgcgcggtg acgctggagg tccggcaggg cgagatcgtg
     1681 gggctgctgg ggccgaacgg ggccggaaag acgacgacgt tctacatgat catggggctt
     1741 gcccgtcccg atcgcggccg cgtctacctc gacgacgccg acctgacgag gcttcccatg
     1801 tacgagcgcg cccgacgggg cattgggtac ctcgcgcagg aggcgtcgat tttcggcaac
     1861 ctgaccgtgg agcagaatct ggaggcggtg ctcgagtacc agggactgcc gaaggccgag
     1921 cgggccgccc gcgcggacgc gctcgtcggc gaattcgggc tggagcgagt gcgccgctcg
     1981 aagggctaca tgctctcggg cggggagcgt cggcgcacgg agatcgcccg agcgctcgcc
     2041 gcgaggccga agttcttcct gctggacgag ccgttcgcag gggtcgaccc catcgcggtc
     2101 gaggacatcc aggcgatcgt cgcggggctc aaggcgcgcg gcatcggcgt cctgatcacg
     2161 gaccacaacg tgcacgagac gcttgccatc acggaccggg cctatctcct ctacgagggc
     2221 cgcatcctga aggccggcac ggcggaagcg ctcgcctcgg atcccgaggt ccgcaagcgg
     2281 tacctgggcg agaagttcac gctcgaacgc taccgctgag gaactggacc ccggcaaccg
     2341 ggtctgaaac cgcccgattt cgacgccgcg ccaccgaccg aaccatgaac cctgcagccg
     2401 atccttccgc cgtcgtcacg ccgcccctgg cggacctgca tgccgatctc aggtggcgcg
     2461 gcatggtgta cgacgcgacg ccgggactgg acgaggtact ccgcacggag cgcctcacgg
     2521 cctacatcgg cttcgatccg acggcttcga gtctccacgt cggctcgatg ctccccatca
     2581 tggggctggc ccggcttcag cgctgcggcc acacgcccat cgccatcgtc ggcggcggga
     2641 cgggcctcat cggcgatccg agcggcaaga cggccgagcg acgcctgctc gatgcggcgc
     2701 aggtcgagga gaacctccag ggcatacgcg cgcagctcga gcgcttcctg gacttcgacg
     2761 cgcggggcaa cccggcgcgg atcgtgaaca acctggactg gctgggcgag ctgcggctgg
     2821 tcgacttcct ccgcgacacg ggcaagcact tcacggtcaa ctacatgctc gcgaaggaat
     2881 ccgtgaagcg ccgcctggag gcggaagacg gaatctcgta caccgagttc acctacatga
     2941 tgctgcaggc ctacgactac ctggtcctgc acgaccggta cggctgcacc ctgcagatgg
     3001 ggggcagcga ccagtggggc aacatcctcg cgggctcgga cctgattcgc cggatcaggg
     3061 gcgcccgcgt gcacggcatg gtctttccgc tggtcaccaa cgccgccggc accaagttcg
     3121 gaaagacgga ggccgggacg gtgtggctcg atccgcagcg gacctctccc taccggttct
     3181 accagttctg gctgaacacg gacgatcgcg acgtcgtggc gtacctgaag tacttcacct
     3241 ggctcgacgg cgcggcggtg gacgcgctcg aggagtccgt ggccgaccgg ccggagagcc
     3301 gggaggccca gcggacgctc gcccgcgagg tgacgacgct cgtgcacggc gagccggaga
     3361 cccgccgcgc ggagcgcgcc gccgcggtgc tgttcggggg cgatctggac gagctctccg
     3421 cccacgacct cctggacgtg ttcgacgacg tcccgtcggc cgacatggac cgggagacgt
     3481 tcgcggggga cggcctgccg ctcatcgacc tcctggccga ctcgggcctg acggcatcga
     3541 aaggggaggc acgccgcctc gtccgctccg gcggggtgtt cgtgaacaac cgtcgcatga
     3601 cggacgagca ggcccgcgtc ggcatcgact cggcggtcga cgggcttctg ctcgtgctcc
     3661 ggaaggggaa gaagaactac cggctcgtca agatccgctg acacacgggg agggattcgc
     3721 catggtcatc gtcatgcgct cgggcgccgg tgaggcccag atccatgccg tgatcgaccg
     3781 gcttaacgcc ttcggattcg acgtgcaccg gtccagcggc ctgcagcgca cggtgctcgg
     3841 ggcgatcggg gccaagcccg gcttcgacat ccggcacatc gcgtcgctcg atggcgtgga
     3901 ggccgtgcac cgggtcacgg aaccgtacgg tcgcgtgctc cggcgcatgg atgatccgga
     3961 gccacggtgg tcgatcggga gccgcgtgtt cggccccgga cgcattgccg tcgtggtgtc
     4021 gctcgtagat gcggacgtcc acgccgaggc gatcgcggcc gtcgtccggt cgggcgcgga
     4081 cgccctgagc cttccggcgc cggcatggag cgcggaagcc ggagccgcgg cgtccggggc
     4141 gggcctgccg gtcctgatcg agatcgccca cggcgcccag cttcccgctg cggacgttcg
     4201 ccccgccgcc tggcgcctga ccggcacggc gatgttcgag cgggacctgc ggcatcgtat
     4261 agcggcgctc ggagcgcccg tcatcgtgga ccgggcgccc gggtccacca tcgaggagtg
     4321 gctgctcgcg gcggatgacc tgctgggagc cgggtgcacg gatgtcgccc tgtgcgattc
     4381 ggggtcccgc tcgctggacc cggcccgctc gctcgcgccc gacatcgcgg ccctggtctt
     4441 cgccgccgtg cgcacacggc ttccggtgct tgcggatccc ggccgcgcga tcgccgatcc
     4501 ggccatggcg ctggcggcgg cgtgcgcggc ggtgggggcc ggcgcccggg gcatcgtgct
     4561 cacactcagg gaccgcaggg gggagcccga cgtggccgtc ctggagcgcg ccggcgagag
     4621 gctgcgcgcg atcgcggcgg ccgtcggccg gtagggcgct ggaccgccgg gtgcgttcgg
     4681 cagccccgaa ccccccgggg gcaggcagcg ttcatgaggg tcctttcccg tcccgagatg
     4741 atcgccgatc gagtcctgaa cgccatcctg gatcgccacc gcgaggtgaa cgacatgatg
     4801 gcagacccca acgtggccgt caacgtctcc cgcatgaagg agctggggcg cgagcatacg
     4861 gcgctgcgcg acgtggtttc cggcatcgag aagtaccggg ccatgctgcg ggaacgcgac
     4921 gacctgcgat cgctcgtgga gtcggaggac gacgccgagt tcgtgcgcat ggcacgcgcc
     4981 gagctctcgg acctggagga ccgcattccg gaattcgagg aggagcttcg cctgcagctg
     5041 ctgccccgtg acccggacga ttcaaaggac tgcatcgtgg agatccgggc cggtacggga
     5101 ggggacgagg cgtgcctgtt tgccggagac ctgtaccggc tctattcccg cttcgccgaa
     5161 ggaaaggggt ggaagctgga tctgatcgat gcctcggagg ggccgcaggg gggcttcaag
     5221 gagatcatct tcggtctctc gggagcggac gccttcggca cgatgaagta cgagagcggc
     5281 gtccaccgcg tgcagcgggt cccggtgacg gagtcgagtg gtcggatcca cacgtcggcc
     5341 gctaccgtgg ccgtgctgcc ggaggcggag gagttcgacg tccagatcgg gccgaacgac
     5401 ctcaagatcg acgtgtaccg ctcgagcggt ccgggcgggc agagcgtgaa cacgaccgat
     5461 tcggccgtgc gcatcaccca cctgccgacc ggcctcgtgg tcacctgcca ggacgagaaa
     5521 agccagcaca agaacaagga caaggccatg cgcgtcctgc ggtcaaggct gtacgagcag
     5581 gagctggagc gccgtaagtc cgagcgctcc gaagcccgtc
//
LOCUS       DBNHQZ010000011         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_137490, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000011 DBNHQZ010000000
VERSION     DBNHQZ010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_137490"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="AC16YU_00305"
     CDS             complement(<1..207)
                     /locus_tag="AC16YU_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6063690.1"
                     /translation="MDRTEIQQRVTGVVASVLAIDPAEVLPGSNFVFDLGADSMKSIE
                     LIAGFEEEFEIEMDEDRAREVQTVE"
     gene            503..1309
                     /locus_tag="AC16YU_00310"
     CDS             503..1309
                     /locus_tag="AC16YU_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6063691.1"
                     /translation="MSQEQPMEQAWTPEHDLALIFIALAYGTDAEFTNHEIEHVTEAL
                     SRWRPDEPQANVREIVIEALAMFEHDSTGDEVVRAIETLANRLDAPSRERALEDVMHI
                     AEADGLLLSGERSLISVIAAAWDLRGTETDLLARSSAAVDDRPVWSLLHDVALLAIAV
                     AHGSTGTLSGGELDRMVDRLRSWRMDLDDDDVRGIIGAAITAYRDGDLKDEIQRSARS
                     IKERLPHAMRLIVIDDIIAVAEADGPMSAAERDMIGSLAQAWHVGVRFEA"
     gene            1499..>2354
                     /locus_tag="AC16YU_00315"
     CDS             1499..>2354
                     /locus_tag="AC16YU_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6063692.1"
                     /translation="MVPEDATRENPPREVLVTGSASQALATRLASPDTNLVEAFLHRF
                     DNERTRRSYRNDLRQFFGMESVSLHVASQVTFFHVNEYLGVLEREGRKPSTLRRRLAA
                     VRGFFDWLDALDLVPDNPTKKQLLRKLPRIRTVDRPILVLTAAQASRLVEAASEPDDV
                     GPRDEALVKVLLYLVLRRSEAAAMDVEHIRPLGSYWILDLPDTKGGPGQYVKVPDHLV
                     ADVDRYRAHYGILSGPLWRSHSRSSRGHRLTPHSIYRIVRSAAQRAGLPEIGAHTLRH
                     TGCTLAIEA"
BASE COUNT          391 a          785 c          801 g          377 t
ORIGIN      
        1 ctcgaccgtc tggacctcgc gggcccggtc ttcgtccatt tcgatttcga actcctcctc
       61 gaaaccggcg atgagctcga tgctcttcat cgaatcggcc cccaggtcga agacgaagtt
      121 cgatcccgga aggacctcgg cagggtcgat cgccaggacg ctggcgacca cgccggtgac
      181 gcgttgctgt atttcggtgc ggtccatgcg tgttatcggc ctttgggacg gaacggatcg
      241 atcccggtct gatgtcgggc gggggaatgt tagagcgcgc ccctccccga atcaaccggt
      301 ccggccggcc tacgcgcact ctcgccggct cccggcaggc gggggaggga atcctccacg
      361 tggacgtggg cccgatcccg acaccggcgc gtgatggttg gtgcaacaac caatggcagg
      421 ggcagtccgc gggtgacgca aggtctttct ggggggcgtg cgttatcatg aaggtgctga
      481 acgtcatcca ccccgagaaa acgtgtccca agagcagccc atggagcagg cgtggacccc
      541 ggaacacgac ctggcgctga tcttcatcgc gctggcgtac ggaacggacg ccgagttcac
      601 gaatcacgag atcgagcacg tgacggaggc cctgtcccgg tggcggcccg acgagccgca
      661 ggcgaacgtc cgggagatcg tgatcgaggc cctggccatg ttcgagcacg attcgacggg
      721 tgacgaggtg gtgcgcgcca tcgaaacgct cgcaaaccgg ctcgacgcgc cgtcacgcga
      781 gcgcgccctc gaggacgtca tgcacatcgc cgaggcggac gggctgctgc tcagcggcga
      841 gcgctcgctc atttcggtca tcgcggccgc ctgggacctt cggggcacgg aaacggatct
      901 gctggctcgt tcgtcggccg ccgtggacga tcggccggtc tggagcctgc tgcacgacgt
      961 cgcgctgctc gccatcgccg tggcccacgg atcgacgggc acgctgtccg gcggagaact
     1021 cgaccggatg gtcgaccgcc tgcggagctg gcgcatggac ctcgacgacg acgacgttcg
     1081 cggaatcatc ggcgccgcca tcacggcgta ccgcgacggt gacctcaagg acgagatcca
     1141 gcgcagtgcg cgttcgatca aggaaaggct tccgcacgcg atgcgcctca tcgtgatcga
     1201 cgacatcatc gccgtcgccg aggcggacgg cccgatgagc gctgccgagc gcgacatgat
     1261 cggctcgctg gcgcaggcct ggcacgtcgg cgtccggttc gaggcgtagg cggatcgcgc
     1321 gtacggtctc gttcggcagg tgaccggaag tccgcgtgga cggggccgga cggaaatccg
     1381 gaagcgctgc tgggcccgat tgcgtcttcc ggacgaatgc aatatcttac gtgtgataat
     1441 aggagttatc gaacacgaat agcctccgga acgcttccgg gagcggtccg aacgacccat
     1501 ggtgcccgag gatgccacga gagagaatcc gccccgcgag gtcctcgtca cgggcagcgc
     1561 ctcccaggcc ctggcgacgc ggctcgcgtc cccggacacg aacctcgtgg aggccttcct
     1621 gcaccggttc gacaacgagc gtacccggcg ctcgtaccgg aacgacctcc ggcagttctt
     1681 cgggatggag tccgtgtccc tgcacgtggc gagccaggtc acgttcttcc acgtgaacga
     1741 gtacctcggg gtgctcgagc gcgagggccg caagccgtcc acgctccgac ggagactggc
     1801 ggcggtgcga ggcttcttcg actggctcga cgccctggat ctcgtaccgg acaatccgac
     1861 caagaagcag ctgcttcgga agctgccgcg gatccggacc gtggaccgac cgatcctggt
     1921 gctcacggcg gctcaggcct cccgcctcgt ggaggccgcg tcggaaccgg acgacgtcgg
     1981 cccgcgggac gaggctctcg tgaaagtcct gctgtacctg gtgctgcgcc gctcggaggc
     2041 cgcggccatg gacgtggagc acatccgtcc tctcgggtcc tactggatcc tggatcttcc
     2101 cgacaccaag ggtggcccgg gccagtacgt gaaggtcccg gatcacctcg tcgcagacgt
     2161 cgaccgctac cgggcccatt acggcatcct ctccggaccg ctgtggcggt cgcacagccg
     2221 ctcgagccgg ggccatcgcc tgacgccgca ttccatctac cggatcgtgc ggtcggccgc
     2281 acagcgcgcc ggactgcccg agatcggcgc gcacacgctg cggcataccg ggtgcacgct
     2341 ggccatcgag gcgg
//
LOCUS       DBNHQZ010000012         3913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2034582, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000012 DBNHQZ010000000
VERSION     DBNHQZ010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2034582"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..701
                     /locus_tag="AC16YU_00320"
     CDS             132..701
                     /locus_tag="AC16YU_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6063693.1"
                     /translation="MPATPDTTAMLIDLRGGNRTVVDRLFPVVYDELQQIAHRLLVGE
                     RSGHTLSTTALVHEAYFRLVDQSRVQWQDRAHFCAVAAKAMRRILIDYARRRRAIKRG
                     GGQQPVSLDEQHAGVDAQADLLLSVDQALESLEKLSDRLARIVELRFFGGMTEEEMAE
                     VLEVSPRTVRRDWVKARAWLYREMYPEKR"
     gene            827..3472
                     /locus_tag="AC16YU_00325"
     CDS             827..3472
                     /locus_tag="AC16YU_00325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6063694.1"
                     /translation="MTSDEWQIIESLFEEALDRPEAEREAWVREHSPDAEICREVLGL
                     IAAAATEDGFLDDPARILAADLIASVERAEHPAQIGPYRPIRELGRGGMGAVFLAERS
                     DGQFEHRVALKMLGRRFPSDDLRRRFLAERQILATLNHPGIARLLDGGVTGEGTLYFA
                     MEFVDGRPIDQYCAEERVPLRERLQLFLQACEAVTYAHRNLVVHRDLKPSNILVEEDG
                     ADPRAARVKLLDFGIAKLLGPDGGPSASPLTVTGMNVMTPEYASPEQVTGGPVTTVSD
                     VYQLGVLLHGLLTGRRLYDFSGRSITEIAHVVGSVEAPRPSSVSDGFPATSDQLKGDL
                     DAIVGKALRKEPETRYESVDRLADDIRRYLDRRPVSAHTGSFAYRARKLVRRHRLAVL
                     AAIVFLVAVAGYVVSITALQARTEQERDRAEQYAAFLTDVFSSPDPFTDDPGDREVTV
                     REFFDQAVERVHAEFDSDPELQLSLLGTIADVYAGLNLDEQALALYRDMLDIGTRLHG
                     PRSETVLEALRGIAWRTGDPEAADSLYRLQLDIAREIEGRTGPLTARSLVPYGSVLAG
                     RDRHEEAERMLQEAAAVFREEGPEAERDLVGALFFLGQTRRTLGRLALADTVLREAYE
                     LRARHYGPTHVRTAILMTELGMVAERLGDLEKATEWKQGALDIFSAELGDEHSYTLSA
                     MNNVAILLDTQGRAEEAEQMIRRVYAARESLYGADHRETVGSLQNLGAFLLKQGRLDE
                     AEPILEEVRVRYARTLPEGHHVHAYPLLSLTELRLRLGDHAGAEAVAREAVSILRSGL
                     SEEHPLTGIAESRLGETLVAQHHLAEAEPLLLEGYRKLRSASGFEGRRQSAIRRLMDL
                     YDAWGRTAEADRYRALLEEGEGEDR"
     gene            3563..>3913
                     /locus_tag="AC16YU_00330"
     CDS             3563..>3913
                     /locus_tag="AC16YU_00330"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6063695.1"
                     /translation="MTVDWQGVFPAVTTKFKPDLSIDEEANRAHLRAQLAAGVHGVIV
                     SGSLGEASTLETDEKLALVSMIADEAAGRIPVVCGVAERTTRNACRFVERAADAGADG
                     FMLLPPMLYPTDRRE"
BASE COUNT          627 a         1253 c         1411 g          622 t
ORIGIN      
        1 gagagggaga ccgtttccgt ttatgccggc tgcatcctgg tagggagcgc gctcccgata
       61 acgccccggg tgtaccattt cttggccgca agtcgttctt tgcggcggcc cggtcgacaa
      121 accacgcgga catgcccgca acgcccgaca cgaccgcaat gctcatcgac ctccggggcg
      181 gcaaccgcac ggtggtggac cggctgttcc cggtggtgta cgacgagctg cagcagatcg
      241 cacaccggct gctggtgggc gagaggagcg gacacacgct gtccaccacg gctctcgtgc
      301 acgaagcgta cttccgtctc gtggaccagt cccgggtcca gtggcaggac cgggcgcact
      361 tctgcgcggt cgcggcgaag gcgatgcgcc ggatcctcat cgactacgcg cggcgccgca
      421 gggcgatcaa gcgcggcggt ggccaacagc cggtctcgct cgacgagcag cacgccgggg
      481 tcgacgccca ggcggacctt ctgctctccg tcgaccaggc gctggagtcg ctggaaaagc
      541 tcagtgaccg cctggcccgg atcgtcgagc tgcgcttctt cggcggcatg accgaagagg
      601 agatggccga ggtcctcgag gtgtccccgc gcacggtccg gcgcgactgg gtgaaggcac
      661 gcgcgtggct gtaccgggag atgtacccgg agaagcggta aatgcggcgg aagcggccgg
      721 gaagaatgcg catctccgca tctgtcggtg gtgttcccgg gccgcgtcct gcgtttcatc
      781 aggattgcgc gtttattgca tccccgagga tccgtcacgt acccccatga cgtccgacga
      841 gtggcagatc atagaatccc tgttcgagga agccctggac cgaccggagg cggagcgcga
      901 ggcgtgggtg cgtgagcaca gccctgacgc cgagatctgc cgggaggtgc tcggcctgat
      961 tgcggcagcg gcgacggagg acggattcct ggacgatccg gcgcggatcc tcgccgcgga
     1021 tctcatcgcc tccgtggagc gggcggagca cccggcccag atcgggccat accggccgat
     1081 ccgcgagctc ggtcggggcg gcatgggcgc cgtcttcctg gcggagcggt cggacggaca
     1141 gttcgagcac agggtcgcgc tcaagatgct cggacgccgt tttccgtccg acgacctccg
     1201 ccggcgattt ctggcggagc gccagatcct cgccaccctg aaccacccgg ggattgcgcg
     1261 gctgctcgac ggtggcgtca ccggggaagg gacgctgtac ttcgcgatgg agttcgtgga
     1321 cggtcgcccc atcgaccagt actgtgcgga ggagcgggtg ccgctgcgcg agcgcctcca
     1381 gctgtttctg caggcctgcg aggcggtcac gtacgcccac cgcaacctgg tcgtccaccg
     1441 cgacctgaaa ccgtcgaaca tcctggtgga ggaggacggg gctgatccgc gggcggcccg
     1501 cgtgaagctg ctcgacttcg gcatcgcaaa gcttctcggc cccgacggcg ggccgtcggc
     1561 atcgcccctg acggtcaccg ggatgaacgt gatgacgccc gagtacgcct cccccgagca
     1621 ggtgacggga gggccggtca cgaccgtctc cgacgtgtac cagctcggtg tcctgctcca
     1681 cggcctgctg accgggcgga ggctgtacga tttctcgggc cgctccatca cggagatcgc
     1741 gcatgtcgtc ggcagcgtgg aggctccgcg gccttcctcg gtgtcggacg gcttccccgc
     1801 cacctcggat cagctgaagg gcgacctcga tgcgatcgtg ggcaaggcct tgcggaagga
     1861 gccggagacc cgctacgagt ccgtggaccg gctggcggat gacatccgcc ggtacctcga
     1921 caggagaccc gtgagcgcgc acacggggtc cttcgcgtat cgagcccgga agctcgtgcg
     1981 gcgccaccgc ctggcggtgc ttgccgccat cgtgttcctg gtcgccgtcg cgggctacgt
     2041 ggtctcgatc acggccctgc aggccaggac cgagcaggag cgggatcggg ccgagcagta
     2101 tgcggccttt ctcacggatg tgttctcgag ccccgacccg tttaccgacg acccgggaga
     2161 ccgggaggtc acggtcaggg agttcttcga ccaggcggtc gagcgggtcc acgcggagtt
     2221 cgacagcgat ccggagttgc agctgtccct gctcggcacg atcgcggatg tgtacgcggg
     2281 tctgaacctg gacgagcagg cgcttgccct gtatcgggac atgctcgaca tcggcactcg
     2341 gctccacggt cccaggtcgg aaacggtctt ggaggcgctc cgcggcatcg cgtggcgcac
     2401 cggcgatccg gaggctgcgg attcgctgta tcggctccaa ctggacatcg cccgcgagat
     2461 cgaggggcgg accggtccgc tgacggccag gagcctcgtc ccgtacggaa gcgtcctggc
     2521 gggacgggac cgccacgaag aggccgagcg catgctgcaa gaggccgcgg cggtgtttcg
     2581 cgaagagggg cccgaggcgg aacgtgacct cgtcggcgca ctgttcttcc ttggacagac
     2641 gcgccggacg ctcggccgcc tggcgttggc cgatacggtg ctcagggagg cctacgagct
     2701 ccgtgcgcgg cactacgggc cgacccacgt gcgaacggcc atcctgatga ccgaactggg
     2761 aatggtcgcc gagcggctcg gtgacctgga gaaggccacg gagtggaagc agggggccct
     2821 ggacattttt tcggccgaac tgggtgatga gcactcgtac acgctctccg cgatgaacaa
     2881 cgtggcgatc ctgctggaca cgcagggccg ggccgaagag gccgagcaga tgatccgtcg
     2941 cgtgtacgcg gcccgcgagt ccctgtacgg agccgatcac cgcgaaaccg tgggcagtct
     3001 gcagaacctg ggtgcgttcc ttctgaagca gggccgattg gacgaggcgg agccgatctt
     3061 agaggaggtc cgggtgcggt acgcgcggac cctgcccgag gggcatcacg tgcatgcgta
     3121 tccgctcctg tcgctgaccg aactgcgcct ccggcttgga gatcacgcgg gggcagaggc
     3181 cgtggcgcgg gaggccgtat cgatccttcg ctcaggactt tcggaggagc accccctgac
     3241 gggcatcgcg gagagccggc tgggggagac cctggtggcg cagcaccacc tcgcggaggc
     3301 ggaacccctg ctcctcgagg ggtatcggaa gctgcggtcc gcgagcgggt tcgaggggcg
     3361 ccggcagagc gccatccgtc gtctgatgga tctatacgac gcctggggcc gcacggcgga
     3421 ggccgaccgg tatcgtgccc ttctcgagga gggggagggg gaggaccgct gaggcaccgc
     3481 ccgggatgat cgatgatcgc ctgttgtgcc actggcacag tagtgatata ttagttgtgt
     3541 ttccacaagc cgtgccgtag aaatgaccgt ggactggcaa ggggtttttc cggcggtgac
     3601 cacgaagttc aagcccgacc tctcgatcga cgaggaggcg aaccgggcac acctccgcgc
     3661 gcagcttgcg gcgggagtgc acggagtgat cgtgagcggg tcgctcggag aggcctcgac
     3721 gctcgagacg gacgagaagc tggcgctcgt ctcgatgatc gccgacgagg ccgcgggcag
     3781 gatccccgtc gtctgcggcg tcgcggagcg caccacgcgg aatgcctgcc ggttcgtgga
     3841 acgcgccgct gatgcgggcg cggacggctt catgctgctc ccgccgatgc tctatcccac
     3901 cgaccgcagg gaa
//
LOCUS       DBNHQZ010000013         3325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_138027, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000013 DBNHQZ010000000
VERSION     DBNHQZ010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_138027"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16YU_00335"
     CDS             complement(<1..342)
                     /locus_tag="AC16YU_00335"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046132 - pyrimidine ribonucleoside
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6063696.1"
                     /translation="MAPERTTDILAGELAGDLLRIGAVALSPDRPFTWASGLRSPVYC
                     DNRLTLGHPAVRRRIADGFVSVLRSAGLRPDVVAGTATAGIPHAAWLADRLDLPMVYV
                     RSAAKGHGKARR"
     gene            complement(332..904)
                     /gene="pgsA"
                     /locus_tag="AC16YU_00340"
     CDS             complement(332..904)
                     /gene="pgsA"
                     /locus_tag="AC16YU_00340"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6063697.1"
                     /translation="MKHLPNALTIVRILVTPVVLALLFVDTAFARGWALFLFVAASIS
                     DWLDGKVARRYAVDTRLGQFLDPLADKVLVLGTFAALAVRWPELVPWWAVAVIAARDV
                     AVTVLRSRAVARGRPLRTSGAAKTKTAFQLTFLILVLLLLFLELLPPVVASPAASLLY
                     SAFVSALLVATVAATVVTGAAYFSPSRHGA"
     gene            1138..3051
                     /gene="dnaK"
                     /locus_tag="AC16YU_00345"
     CDS             1138..3051
                     /gene="dnaK"
                     /locus_tag="AC16YU_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR6063698.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPVVITNSEGARTTPSIVAFKK
                     DGERLVGAPAKRQAITNPAHTVSSIKRFMGRQYGEVATEIGEVPYAVVHGENDTARVK
                     IDDRVYTPQEISAMVLQKLKQTAEDHLGEKVTEAVITVPAYFNDAQRTATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKKEKDEIIAVFDLGGGTYDISILELGEGVFEVKSTNGD
                     THLGGDDFDQRLIDWIADEFKREEGIDLRKDAMALQRLKEAAEKAKIELSSSTQTTIN
                     LPFITATQEGPKHLTLDLTRALFEKLVDDLVQRTLPPMEKALKDAGISRERIDEVILV
                     GGSTRIPIIQQVVEKFFGKKPNRSVNPDEVVAVGAAIQGGVLSGDVSDVLLLDVTPLN
                     LGIETLGGVTTVLIASNTTIPTRKSETFSTASDNQPSVEIHVLQGDRSMATDNRTIGR
                     FHLDGIPPAPRGVPQVEVTFDIDANGILNVSAKDRATGKEQSVRIEASSGLSQQEIEK
                     MRTDAKSHADEDRKRREEADKVNAADQLIFSSEKNLKEYGDKIPAEKKAKIESALERL
                     KEAHKTRNLVEIESSMQQLNEAWNEASQDLYAAQQAAAQGATGEPADETGDASGEVRD
                     VDYEVVDDDESKA"
     gene            complement(3142..>3325)
                     /locus_tag="AC16YU_00350"
     CDS             complement(3142..>3325)
                     /locus_tag="AC16YU_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6063699.1"
                     /translation="VSLEVYDLTGSLVESIFENRRLAQGTYTERFDASGLASGIYLYR
                     LQGAGFVLTRAMTVVK"
BASE COUNT          637 a         1131 c         1152 g          405 t
ORIGIN      
        1 ccggcgggcc ttcccgtggc ccttcgcggc cgagcggacg tagaccatcg gcaggtccag
       61 gcggtccgcc agccaggccg cgtgcgggat gccggcggtc gccgtgcccg ccacgacgtc
      121 cgggcgaagg cccgcggacc gcaggaccga gacaaacccg tcggcgatgc gccggcgcac
      181 ggccgggtgg ccgagcgtca gccggttgtc gcagtatacc ggggagcgca ggcccgaggc
      241 ccacgtgaac gggcggtcgg gcgagagggc caccgcgccg atccggagga gatctccggc
      301 gagctcgccg gcgaggatgt ccgtcgtccg ctcaggcgcc atgtcgggag ggagagaaat
      361 acgcggcgcc cgtcaccacc gtcgccgcga cggtcgccac gaggagcgcg gagacgaatg
      421 cgctgtagag caggctcgcc gccggcgagg cgacgacggg agggagaagc tccaggaaga
      481 gcagcaggag caccaggatg aggaatgtga gttggaaggc cgtcttggtc ttcgcggctc
      541 cggacgttct gagtggccgc ccgcgggcca cggcccgcga ccgcaggacg gtcacggcca
      601 cgtcgcgggc cgcgatcacg gcgacggccc accacgggac gagttcgggc caccggacgg
      661 ccagggccgc gaacgtcccg aggacgagca ccttgtcggc gagcggatcc aggaactggc
      721 cgaggcgcgt gtccaccgcg tagcgccgcg cgaccttccc gtcgagccag tccgagatcg
      781 aggcggcgac gaacagaaac agggcccacc cgcgggcgaa cgcggtatcg acgaacagca
      841 gcgccagcac gacgggcgtg accaggatgc ggacgatcgt gagcgcgttc gggaggtgtt
      901 tcatcgcgcg gaaacgacgg gcgccgggca atatacggtc tgcctcggcg tcgcgtgatc
      961 ctgcaattcg tgcaggcgat gcggggccga aggcgcgtct gcgggcgtcc tttcgcgccg
     1021 gtcctgacac aatgtccgcc cggagagttg ggcacccttc ttgcgcacgc tgccatgcag
     1081 cgcggcacgg gcgtaccggg tcgcccaccc cgaatcacct gttcaagagt acctggcatg
     1141 ggaaagatca taggaatcga cctcggcacg acgaactccg tcgtggccgt catggagggc
     1201 ggtgagcccg tcgtcatcac gaactcggag ggcgcgcgca cgactccgtc catcgtcgcc
     1261 ttcaagaagg acggcgagcg cctggtcggc gcgccggcca agcggcaggc catcacgaat
     1321 cccgcgcaca ccgtctcctc gatcaagcgc ttcatgggcc gccagtacgg cgaggtcgcc
     1381 acggagatcg gggaagtccc gtacgcggtc gtgcacggcg agaacgacac ggcccgcgtc
     1441 aagatcgacg accgcgtgta cacgccgcag gagatctcgg cgatggtcct gcagaagctg
     1501 aagcagacgg ccgaggacca cctcggcgag aaggtcaccg aggccgtgat cacggtgccc
     1561 gcgtacttca acgacgcgca gcgcaccgcc accaaggagg ccggcgagat cgccgggctc
     1621 aaggtccgcc gaatcatcaa cgagcccacg gccgcggcgc tcgcgtacgg gctcgacaag
     1681 aaggagaagg acgagatcat cgccgtcttc gacctgggcg gcggcacgta cgacatctcg
     1741 atcctggagc tgggcgaggg cgtattcgag gtcaagtcca ccaacggcga cacgcacctc
     1801 ggcggcgacg acttcgacca gcgcctcatc gactggatcg ccgacgaatt caagcgcgag
     1861 gagggcatcg acctgcgcaa ggacgcgatg gccctgcagc gcctgaagga ggcggccgag
     1921 aaggcgaaga tcgagctctc gtcctcgacc cagacgacga tcaacctgcc tttcatcacg
     1981 gccacgcagg aggggccgaa gcacctcaca ctcgacctga cgcgcgccct gttcgagaag
     2041 ctcgtcgacg acctggtgca gcggacgctc ccgcccatgg agaaggccct caaggacgcc
     2101 ggcatcagcc gtgagcgcat cgacgaggtg atcctggtcg gcggctccac gcgcatcccg
     2161 atcatccagc aggtggtcga gaagttcttc gggaagaagc cgaaccgctc ggtgaacccg
     2221 gacgaggtcg tcgccgtcgg cgcggccatc cagggcggcg tgctctcggg cgacgtgtcc
     2281 gacgtcctgc tcctcgacgt cacgccgctg aacctgggca tcgagaccct cggcggcgtc
     2341 accacggtgc tcatcgcgtc gaacaccacg atcccgaccc gcaagagcga gacgttctcg
     2401 acggcgtccg acaaccagcc ctccgtcgag atccacgtcc tgcagggcga ccggtccatg
     2461 gcgaccgaca accgcacgat cggccgcttc cacctcgacg gcatcccgcc ggcgcctcgc
     2521 ggcgtgcccc aggtcgaggt caccttcgac atcgacgcca acggcatcct gaacgtgtcg
     2581 gccaaggacc gggctaccgg caaggaacag tcggtccgca tcgaggcctc gtcgggcctg
     2641 tcgcagcagg agatcgagaa gatgcgcacc gacgcgaagt cccacgccga cgaggaccgc
     2701 aagcggcgcg aggaggcgga caaggtcaac gccgcggatc agctcatctt cagctcggaa
     2761 aagaacctga aggagtacgg cgacaagatc cccgccgaga agaaggcgaa gatcgagagt
     2821 gcgctcgagc gcctcaagga ggcgcacaag acgcgcaacc tggtcgagat cgagtcttcc
     2881 atgcagcagc tgaacgaggc gtggaacgaa gcgagccagg acctctacgc cgcccagcag
     2941 gccgccgccc agggcgcgac cggcgagccg gcggacgaga ccggcgacgc ctcgggcgag
     3001 gttcgcgacg tcgactacga ggtcgtggac gacgacgagt cgaaggcctg atccggcgga
     3061 gcgcccgtaa cgacgaaggg acccggagcc gcgcggcacc gggtcccttt ccattccatc
     3121 cgaccgaggg ccgggagact cctacttcac gacggtcatc gcccgcgtga gcacgaatcc
     3181 cgctccctgc agccgataca ggtagatgcc actcgccagg ccggaggcgt cgaagcgctc
     3241 cgtgtacgtg ccctgcgcca ggcgccggtt ctcgaagatc gactcgacga gcgagcccgt
     3301 caggtcgtag acctcgaggg acacg
//
LOCUS       DBNHQZ010000014         3480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1899363, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000014 DBNHQZ010000000
VERSION     DBNHQZ010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1899363"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="AC16YU_00355"
     CDS             <1..934
                     /locus_tag="AC16YU_00355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HIR6063700.1"
                     /translation="QRVAVRQLEGRYSELLGRLRDELLDVTGYVELELDFSEEDVAFA
                     DRPRLSALLVDAGRTLERLVDSGRAGRMTRDGVRVVIAGRPNAGKSSLMNALVGHDRA
                     IVSEEAGTTRDSIEADLELDGFLFRIVDTAGLRAASGGVEAEGIRRTGREMRQADVIL
                     YVFDASVGLADGELSALGIDHPDTPVVLVANKRDLVGGGIEPGAVPVSATRAMHDERE
                     LDPLLDALRGSLDPVLRAVDASPALANARHREHLHRAREAVGRAHGALGEGRTGDLVA
                     QDLRLALHELGCITGAVTSEDVLGHIFSRFCVGK"
     gene            988..2898
                     /gene="mnmG"
                     /locus_tag="AC16YU_00360"
     CDS             988..2898
                     /gene="mnmG"
                     /locus_tag="AC16YU_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR6063701.1"
                     /translation="MPLPSRFDVIVVGAGHAGAEAAHAAARMGCTTLLVTLSLDAVGR
                     MSCNPAIGGIGKGQIVREIDALGGLMGLAADRAGIQFRMLNRRKGPAVWGPRAQCDRL
                     EYATVIREMLEDTPGLYLRADMVTDLLVDGDRVRGVRTQLGVDIPASAVILTSGTFMN
                     GVIHVGDRQFGGGRMGERASTGLTGALERLGFESGRLKTGTPPRLDGRTIDYAQLEEQ
                     PGDADPAPFSFLSGRVGARQVSCWLAYTSPAVHDMLRTGFDRSPMFAGRIRGTGPRYC
                     PSIEDKIDRFADRDRHQLFLEPEGLRTSEVYVNGFSTSLPEEVQERAIRLVPGLENVH
                     VLRPGYAIEYDYFPPFQVRFSLETRRVRGLFFAGQINGTTGYEEAAAQGLMAGINAVL
                     GIRGEEPLVLRRSEAYIGVLIDDLVTKGTEEPYRMFTSRAEHRILLRQDNADFRLTEL
                     GHELGLASDERLRRTRARREAARITGDRLRTTTVSPDAVNDYLEAAGTATIAEPERLG
                     RLALRPEIDVSALLRHAGLYESIVAPWDGPEPVIRTVESDLKYAGYLERERSHVAQMS
                     RLEDLRIPDGIGYEEIRALSIEAREKLTRIRPETLGQASRISGVSPADVSVLMVMLRS
                     PGSVSRETSGSP"
     gene            2895..>3480
                     /locus_tag="AC16YU_00365"
     CDS             2895..>3480
                     /locus_tag="AC16YU_00365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6063702.1"
                     /translation="MTTVDPLAAIPAGRARLVEVYVEALERANRGVNLVSRASSDTIR
                     IRHVAHSMALALRRFRAGTTVVDWGTGGGLPAVPLAICFPDVEFVAVDSIGKKARALE
                     SIVRETGLENVTVWHGRAEEWDGAAEYAVSRATAPLDRLWAWSRRVLSPVRPGAGEWA
                     GGLLCLKGGDLDPERRGLGRLDPSARVETVDLART"
BASE COUNT          498 a         1159 c         1326 g          497 t
ORIGIN      
        1 gcagcgggtc gcggtccggc agctggaggg ccggtactcg gagctgctcg ggcggctgcg
       61 cgacgagctc ctggacgtga ccgggtacgt ggagctggag ctggactttt ccgaggagga
      121 cgtggcgttc gccgatcggc cccggctgtc ggccctgctc gtggacgccg gccggacact
      181 ggagcggctc gtggactcgg gccgcgcggg ccggatgacg agggacgggg tgcgcgtcgt
      241 gatcgcgggc cgtccgaacg ccggtaagtc ctcgctcatg aacgcgctcg taggccatga
      301 ccgcgcgatc gtcagcgaag aggccgggac gacgcgggac agcatcgagg cggacctgga
      361 actggacgga ttcctgttcc gcatcgtgga cacggcgggg ctccgcgcgg cgtccggcgg
      421 cgtcgaggcc gaggggattc gcaggactgg gcgtgaaatg cggcaggccg acgtgattct
      481 gtacgttttc gacgcgagcg tcggcctcgc ggacggcgaa ttgtcggcgc tcggcatcga
      541 ccacccggac acgccggtcg tcctggtcgc gaacaagcgg gacctggtcg gaggcgggat
      601 cgagcccggt gccgtaccgg tgagcgcgac gagggcgatg cacgacgagc gggagctgga
      661 tccgctgctc gacgctctgc ggggatcgct ggacccggtg ctgcgggcgg tggacgcctc
      721 gcccgcgctc gcgaacgcgc gccaccggga gcatctgcac cgggcgcgcg aggccgtggg
      781 ccgcgcccac ggggccctcg gcgaggggcg gacgggagac ctcgtggcgc aggacctgcg
      841 cctggccctg cacgagctcg gctgcatcac gggagcggtc acgagcgagg acgtgctcgg
      901 acacatcttc tcgcggttct gcgtcggcaa gtgaaccggg aacggaggcc cgccgggctc
      961 cgtgcaacgg ccctcatccc tcgatccatg cccctcccct cccgcttcga cgtcatcgtg
     1021 gtgggcgccg gccacgccgg cgccgaggcc gcgcacgccg cggcgcgcat gggatgcacg
     1081 acgctcctcg tgaccctgag cctggacgcg gtgggccgca tgtcctgcaa tccggccatc
     1141 ggcggcatcg gcaagggcca gatcgtccgg gaaatcgatg ccctgggcgg cctgatgggc
     1201 cttgcggccg accgggccgg catccagttc cggatgctga accggcgcaa gggtccggcg
     1261 gtctggggcc cgcgggccca gtgcgaccgc ctcgagtacg cgaccgtgat ccgggagatg
     1321 ctcgaggaca cgccgggcct gtacctgcgc gccgacatgg tgacggacct cctggtcgac
     1381 ggcgaccgag tccggggcgt gcggacccag ctcggggtgg acatcccggc gtctgcggtg
     1441 atcctcacga gcgggacgtt catgaacggc gtgatccacg tcggcgaccg ccagttcggc
     1501 ggcggaagga tgggcgagcg cgcctcgacc ggtctcacgg gcgcgctcga gcgccttggc
     1561 ttcgaatcgg gacggctcaa gacgggcacc cctccccggc tcgacggccg caccatcgac
     1621 tacgcgcagc tcgaggagca gccgggcgat gccgatccgg cgcccttttc gttcctgtcc
     1681 ggccgggtgg gcgcgcggca ggtgagttgc tggctcgcgt acacgagccc cgcggtccac
     1741 gacatgctgc ggacggggtt cgaccgcagc cccatgttcg cgggacggat ccgggggacg
     1801 gggccgcggt actgcccgtc gatcgaggac aagatcgacc ggttcgcgga ccgggaccgt
     1861 caccagctgt ttctggagcc ggaagggctg cggacgagcg aggtgtacgt caacggcttc
     1921 tcgacgagcc tgccggagga ggtgcaggag cgggcgatcc ggctcgtgcc gggcctcgag
     1981 aacgtgcacg tgctccggcc gggctacgcg atcgaatacg actacttccc ccctttccag
     2041 gtgcggttca gcctcgagac gcggcgcgtc cgggggctct tcttcgcggg gcagatcaac
     2101 ggcacgaccg gctacgaaga ggccgcggcg caggggctga tggcgggaat caacgcggtc
     2161 ctcggtatcc gcggcgagga gcccctggtc ctgcgccggt cggaggcgta catcggggta
     2221 ctgatcgacg acctggtgac gaagggcacc gaggagccgt accgcatgtt cacctcgcgc
     2281 gcggagcacc ggatcctgct ccgccaggac aacgccgatt tccgtctcac ggagctgggc
     2341 cacgagctgg gactcgcgag cgacgaacgc cttcggagga cacgcgcgcg gcgggaggcc
     2401 gcgcggatca ccggggatcg cctccggacg acgaccgtgt cgcccgacgc ggtgaacgac
     2461 tacctggaag cggccggcac cgcgacgatc gcggagcccg agcgccttgg gcggctggcg
     2521 ctccgccccg agatcgacgt gtcggcactg ctccggcacg ccggcctcta cgagagcatc
     2581 gtcgccccgt gggacggacc ggaacccgtg atccgcaccg tcgagtccga cctgaagtac
     2641 gcggggtacc tggagcggga acgctcacat gtggcacaga tgtcccggct cgaggacctg
     2701 cgaattccgg acggtatcgg gtacgaggaa atccgcgcgc tgtcgatcga ggcccgggag
     2761 aagctcaccc gcatcaggcc ggagaccctc gggcaggctt cccggatctc cggggtcagc
     2821 ccggccgacg tctccgttct catggtcatg ctccggtccc cgggctccgt ttcacgtgaa
     2881 acatcgggct cgccgtgacc accgtcgatc cgcttgcggc gatccccgcc ggtcgtgcgc
     2941 ggctggtgga ggtctatgtc gaggccctgg agcgggccaa ccgcggggtg aacctggtgt
     3001 cgagggcctc ctcggacacg atccggatcc ggcacgtcgc gcactccatg gcgctggcgc
     3061 tccgccggtt tcgggccggg acgaccgtgg tggactgggg aactggcggc gggctccccg
     3121 ccgtgccgct cgccatctgc tttccggacg tggagttcgt ggcggtggac tcgatcggta
     3181 agaaggccag ggcgctcgag agcatcgttc gggagacggg cctggaaaac gtgaccgtct
     3241 ggcacggcag ggcggaggag tgggacggag cggcggaata cgccgtctcg cgggcgacgg
     3301 ccccccttga ccggctgtgg gcctggagcc ggcgcgtact cagtccggtc cggccgggtg
     3361 cgggcgagtg ggccgggggg ctgctctgcc tgaagggggg agacctggat ccggagcggc
     3421 gcggcctggg acgcctggac ccgtccgcgc gcgtcgagac cgtcgatctc gcgcgaacat
//
LOCUS       DBNHQZ010000015         8370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3118799, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000015 DBNHQZ010000000
VERSION     DBNHQZ010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3118799"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="AC16YU_00370"
     CDS             complement(<1..298)
                     /locus_tag="AC16YU_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIR6063703.1"
                     /translation="MTQLIRFSPAGDVRRMQNEFDRLFGSLFPTTRSEDPEAETAVWA
                     PRVDLVENDEAYLIHADLPGLSKEDVNVNYHDGVLSISGDRRSEQYEEKDNCVRL"
     gene            complement(501..1403)
                     /locus_tag="AC16YU_00375"
     CDS             complement(501..1403)
                     /locus_tag="AC16YU_00375"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6063704.1"
                     /translation="MTTSEPAFRGVWCASVTPLREDLSIDVDRLERHLGRLLADGCDG
                     VALFGSTGEGNSFSVRERVACILDLSARGMPMDRVIVGAGACAVPDAVFLARAAREAG
                     ASGVMMHPPFYYRGASEDGLFAFLAAVVRRMGREPIPVVLYHFPKLVGVGYGPELVAR
                     LVDHRPDVFVGLKDSSGDLERMAAIAAAHPGFSVLAGTERLLLPLLERGGAGCLTATT
                     NLTAALAQRVFAARRPVDQERLSAAREALETAPFVPGLKRLIADREGDAGWARVRPPL
                     DAAPPDVADRLRAAVAAAPYDVSA"
     gene            complement(1390..2658)
                     /gene="fahA"
                     /locus_tag="AC16YU_00380"
     CDS             complement(1390..2658)
                     /gene="fahA"
                     /locus_tag="AC16YU_00380"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HIR6063705.1"
                     /translation="MLDLFRTPPDHPFPIQNLPWGVFSAGDEGPRAGVRIADSVLDLA
                     AIAGRGLIDVPGLDARRVFGAPVLNPFMASGPGAWSAVRRRIAALVSGESPEFADDSD
                     LRSRAIHAADRVRMHLPAAIGDYTDFYSSREHATNVGTMFRGAENALPPSWLHLPIGY
                     HGRASSVVVDGTPVRRPAGQTRQGDADPAFGPSRLLDFELETGFFVGPGNDLGEPVPL
                     ARAHEQVFGFVLVNDWSARDIQKWEYVPLGPFLGKSFATTISPWVVPLEALLPMRVPG
                     PRQNPAPLPYLRDPEPWSFDVRLEVGIRPAGRDRSTTVCRSNLRHLYWNVRQQVAHHT
                     SGGCNLRPGDLLASGTISGPDPGSFGSLLELTWRGARPLDLGDGVTRRFLEDGDTVIL
                     TGYAQGNGYRIGFGEASGTVVPNDNGHDNE"
     gene            complement(2669..3601)
                     /locus_tag="AC16YU_00385"
     CDS             complement(2669..3601)
                     /locus_tag="AC16YU_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063706.1"
                     /translation="MTDTFRIHPLLDGLVDYAGLFPPTRLDMTASVAEYDAHRRGEAG
                     ALVGRFVCPSGRLAELDAFAALFDAAHPLRLALVVAPEIPLREALREASAFVRAFKGM
                     ARVETAEIRLDDPGTGLALRSRLCDAADAAAPRPVYVELPVAADPEAALRALPEFAPA
                     RRAGRYGLGAKLRCGGERAADYPSPEAVASFLYRCVMDGVPFKATAGLHHPVRGRPSP
                     EGPPMHGFLNVFGAAVLGATRGLAESELLEIVSETDPAAFSLEDGRFQWKEHAAPLPA
                     VHAVRAGIATSFGSCSLDEPADDLRALGWLQAYP"
     gene            3807..5012
                     /locus_tag="AC16YU_00390"
     CDS             3807..5012
                     /locus_tag="AC16YU_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6063707.1"
                     /translation="MKRLSAVFALLVGGLLLMGADGCSSDPNVEGAKLDLRNKDYDRA
                     LENLEIAISKDPANAEALELKGRVLSEMAFATMDVEENVELIGDMIQAFDAALAVDAT
                     MAESIDRSMVIAYVNEFQRGIQAFNRGQDDAGEFNAAATYFGTAATIQPDSAGAYLNQ
                     AYALMNAGRNGDAIVPFEQAIAKGDTEVDTYRFLASLYQTNGRAADAVTVLENVSEMY
                     PNNPDVQAELLNAYQVAGMSDRALQVYEDAVESDPENKLYRYNFGSLLVAAERYDEAI
                     VQLEKAIEIDPEYGNAQYNLGAAYINKAVDVNNRINELDDNLRSNRDSMSEGDIQAAE
                     AEIDRLADERRTLFGSAIGPLERARVLFEAEGESATAVCQALYQSYVQTNQLELAQSI
                     AVCAGYEDN"
     gene            5099..6271
                     /locus_tag="AC16YU_00395"
     CDS             5099..6271
                     /locus_tag="AC16YU_00395"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR6063708.1"
                     /translation="MAADRSATGTRFATLAVHAGQEPDPSTGAIMPPIYQTSTYAQAA
                     PGEHKGYEYARVSNPTRTALEGNLAALEGARHGVAFSSGVAATDAIVKCLRPGDHVVA
                     SNDLYGGTYRLLRQVFGPFGIGSSFVDMSSSEEVERAFRPETRLLWIETPTNPLLRIV
                     DIEALARMGRARGAAVAVDNTFATPWLQRPLALGADLVVHSTTKYLGGHSDVIGGAVC
                     TNDDDWADRLRFLVKSAGAVPGPMDCFLTLRGTKTLHLRMERHCANAARVADFLAGHP
                     AVGKAFYPGMPDHPGHAIARRQMRGFGGMVSFILKDESIEAAFRVLSSTRVFTLAESL
                     GGVESLLNHPATMTHGSIPREERIAAGLHDSLIRLSVGIEDVEDLVEDLDRALRSA"
     gene            complement(6275..>8370)
                     /locus_tag="AC16YU_00400"
     CDS             complement(6275..>8370)
                     /locus_tag="AC16YU_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR6063709.1"
                     /translation="GNGRSGQAVADGSDRHDAPIGDELDFTPVGPEAFEPETLLGVYR
                     TMLTSRRLDEKMLTLLKQGKGFFHIGCAGHEAAQAAVGLLSRPGHDWFSGYYRDLTMY
                     LMLGGTSREVMLHHLAKADDPNSGGRQMSEHYSNRERHILPLSSSVGSQFLPAVGVGL
                     AIRRDPGLDAYVYCSCGDGATSQGEFHEAMNWAARAGIPVLFLVQDNKYAISVPVQDQ
                     TAGGSAYKLARGYEGLRRASVDGTDFFETWSAARAAIESMRSGGGPVCLVADLVRLLP
                     HSSSDNHAKYRSSEELEADRQVDPIVRFEERLVAAGVLDEPAITRIRKEVQTEVDQAA
                     AWAMTQADPDPSTATRHVLFEGDLGLEYEASEPDGEPIVMVDAINHALHEEMERDERI
                     LVYGEDVGGDKGGVFTATRNLTKRFGEDRCFNSPLAEASVVGTAVGLSAAGYKPVVEI
                     QFGDYIWPAASVLRNQVASFRYRSNDTWSCPMVIRVPVGGYIHGGLCHSQNIEAFFGH
                     MPGLVVAFPSTAADAKGLLKTAIRMQDPVLFLEHKALYRAAAARSPQPDADYLLPFGK
                     ARRVREGSDVSVITYGMMVHKAAVAARHLEKEGISVDIVDLRTIVPLDQEAVLESVRR
                     TGRVVVVYEDNGFAGFGAELCAQVADLAFESLDAPVRRVSGAFTPVPFADPLERAVLP
                     DEAHIAAAIRELAAY"
BASE COUNT         1304 a         2898 c         2983 g         1185 t
ORIGIN      
        1 cgaggcggac gcagttgtcc ttctcctcgt actgctccga cctgcggtcg ccgctgatgg
       61 acagcacgcc gtcatggtag ttgacgttca cgtcttcctt ggagagaccc ggaagatcgg
      121 cgtggatcag gtacgcctcg tcgttctcga cgagatcaac tcggggcgcc cagacggccg
      181 tctcggcctc gggatcctcg gagcgcgtcg tcgggaacag ggacccgaac aggcgatcga
      241 actcgttctg catgcggcga acgtctccag cgggcgagaa acggatgagc tgtgtcatgg
      301 gtggcacctc gttagtgcta ttgggttgtg tgtctggttg tcggtcgttg ccgtttggct
      361 cgaagaaccg caaccggcat gccaccgggc cccgcgcatg cattctgaca cgttccgaag
      421 gctcgcatgc gccagcctgt caagcggtgt gaaaggctga cacgccttcg tcccggctac
      481 ctgtcacacc cgcgcacccg ctatgcagaa acgtcatatg gcgccgccgc cacggcggcc
      541 cgaagccggt ccgccacgtc cggcggggca gcgtcgaggg gcggacgcac gcgggcccag
      601 ccggcatcac cctcgcggtc ggcgatgagc cgcttgaggc cggggacgaa gggcgcggtc
      661 tcgagcgcct cgcgggccgc cgagagccgc tcctggtcca cgggccgcct cgcggcgaag
      721 acccgctggg cgagggcggc ggtcaggttg gtcgtcgcgg tcaggcagcc cgcgccgccg
      781 cgctccagca ggggcaggag caggcgctcc gtgccggcga gcacagagaa gcccggatgg
      841 gccgccgcga tggccgccat gcgctcgagg tccccggacg aatccttcag gcccacgaac
      901 acatcggggc ggtggtcgac gagccgcgcg acgagctccg ggccgtagcc gacgccgacc
      961 agcttcggga aatggtacag gaccacgggg atcggctcgc gccccattcg gcggacgacg
     1021 gccgcgagga acgcgaaaag cccgtcttcg gacgcgcccc ggtagtagaa cggcggatgc
     1081 atcatcacgc ccgatgcccc cgcctcgcgg gccgcccgcg cgaggaagac ggcgtccggc
     1141 accgcgcacg cccccgcgcc tacgatcacg cggtccatgg gcattccccg cgccgagaga
     1201 tccaggatgc acgccacgcg ctcccgcacc gagaagctgt tgccctcgcc cgtcgagccg
     1261 aagagcgcga cgccgtcgca gccgtccgcg agcagccgcc ccaggtgccg ttccaggcgg
     1321 tccacgtcga tcgacaggtc ctcgcggagc ggggtcaccg acgcacacca cacgccgcgg
     1381 aaagccggtt cactcgttgt catggccatt gtcattcggt acgaccgtgc ccgacgcctc
     1441 gccgaagccg atccggtacc cgtttccctg cgcgtagccc gtaaggatga ccgtgtcccc
     1501 gtcctcgagg aagcgccgcg tcacgccgtc gccgaggtcc aggggacgcg cgccccgcca
     1561 cgtgagctcg agcaggctgc cgaagctccc gggatcgggc ccgctgatcg tgcccgaggc
     1621 gaggaggtcg cccgggcgca ggttgcatcc gccggacgta tggtgggcga cctgctggcg
     1681 cacgttccag tacaggtgcc tcaggttcga tcggcagacc gtggtcgacc ggtcgcgtcc
     1741 cgccgggcgg atgccgacct cgagacggac gtcgaacgac cacggctccg ggtcgcggag
     1801 gtaggggagg ggcgcgggat tctgccgggg gcccgggacg cgcatcggca ggagagcttc
     1861 gagggggact acccacggac tgatggtggt ggcgaaactc ttgcccagga atggtccgag
     1921 cggcacgtat tcccacttct ggatgtcgcg ggcgctccag tcgttcacga gcacgaatcc
     1981 gaagacctgc tcgtgggccc tcgcgagggg cacgggctcg ccgaggtcgt tgcccgggcc
     2041 tacgaaaaag cccgtctcga gctcgaaatc gaggagccgg ctcggaccga acgcggggtc
     2101 cgcgtcgccc tgccgcgtct gtcccgcggg ccgtcggacg ggcgtgccgt ccacgaccac
     2161 cgaggaggcg cggccatggt acccgatcgg caggtgcagc caggagggcg gcagcgcgtt
     2221 ctcggcgcct cggaacatgg tgccgacgtt cgtcgcgtgc tcgcgagacg aatagaagtc
     2281 cgtgtagtcg ccgatggccg ccggcaggtg catgcgcacg cggtcggccg cgtggatggc
     2341 gcgcgagcgc aggtcggaat cgtcggcgaa ctcggggctt tcgcccgaga cgagcgccgc
     2401 gatccggcgc cggacggccg accaggcccc gggccccgat gccatgaagg ggttcagcac
     2461 aggcgcaccg aacacccggc gcgcgtcgag gcccgggacg tcgatgagcc cgcgcccggc
     2521 gatcgcggcg aggtccagca cgctgtccgc gatgcggacg ccggcccggg gcccttcgtc
     2581 tccggcactg aacacgcccc agggcaggtt ctggatcgga aacgggtggt cgggcggggt
     2641 ccggaagaga tccagcatcg ggctacggtc aggggtaggc ctgcagccag ccgagtgcgc
     2701 gcaggtcgtc ggccggctcg tccaggctgc agctgccgaa cgacgtggcg attcctgccc
     2761 ggacggcatg gaccgccggg agcggcgcgg cgtgctcctt ccactggaaa cgtccgtcct
     2821 cgagcgagaa cgccgcggga tcggtctccg agacgatctc gaggagctcc gattccgcga
     2881 ggccgcgcgt ggcgcccagg accgcggcgc cgaagacatt caggaagccg tgcatcggcg
     2941 ggccctcggg cgagggccgg ccccgcaccg ggtggtgcag accggccgtg gccttgaagg
     3001 gcacgccgtc catgacgcag cggtacagga acgaggccac cgcctcaggg ctggggtagt
     3061 ccgcggcccg ctcgccgccg cacctgagct tggcgccgag gccgtaccgt ccggcgcggc
     3121 gtgccggggc gaactcgggg agtgcgcgaa gggcggcctc cggatcggcg gcgaccggga
     3181 gctcgacgta caccggccgc ggggccgcgg cgtccgcggc gtcgcagagg cgactccgca
     3241 gggcgagacc cgtgccggga tcgtccaggc ggatctcggc ggtctcgacc cgggccatgc
     3301 ctttgaaagc ccggacgaag gccgacgcct cccggagcgc ctcgcggagc gggatttccg
     3361 gggccacgac gagtgcgagg cgcaggggat gtgccgcgtc gaacagggcg gcgaaggcat
     3421 ccagttccgc gagccggcct gaagggcaga cgaagcgtcc cacgagcgcg cccgcctcgc
     3481 cccgccggtg ggcgtcgtac tcggcgacgg aggcggtcat gtccagccgg gtcggcggaa
     3541 agaggcccgc gtagtccacc agcccgtcga gcagggggtg tatgcgaaag gtgtcggtca
     3601 tcggggctcg tcggacggcg aaagcgccgc ggtccaccgc cgagaatacg aaaccttcga
     3661 caactcttga ccgtgcaatt tggccgcgcg cggagtacca ccgtaccttg atcgacaggc
     3721 tgttcgcgcg gggaccgggc ttgtgtccgc aattgcgcgc gttcaaaccc tcagccgacc
     3781 cgaccactct gaccgacctg cccgtcatga agcgtctttc cgccgtcttc gccctcctcg
     3841 tcggtggcct cctcctcatg ggtgccgacg gatgctcgag cgaccccaac gtggagggtg
     3901 ccaagctcga cctccggaac aaggactacg accgcgccct ggagaacctg gaaatcgcga
     3961 tctccaagga tcccgccaac gccgaagccc tcgagctcaa gggccgcgtg ctgtccgaga
     4021 tggcgttcgc cacgatggac gtggaggaga acgtcgagct gataggggac atgatccagg
     4081 cgttcgacgc cgcgctcgcc gtcgacgcca cgatggccga gtcgatcgac cggtccatgg
     4141 tgatcgcgta cgtgaacgag ttccagcgcg gcatccaggc gttcaaccgc ggccaggatg
     4201 acgccggcga gttcaacgcc gcggcgacct acttcggcac ggctgcgacg atccagcccg
     4261 attcggccgg cgcctacctg aaccaggcct acgcgctgat gaacgccggg cgcaacgggg
     4321 acgccatcgt gcccttcgag caggccatcg ccaagggcga taccgaggtg gacacctacc
     4381 gcttcctcgc gagcctctac cagacgaacg gccgggccgc ggacgccgtg accgtcctgg
     4441 agaacgtctc ggagatgtat ccgaacaacc ccgacgtgca ggccgagctg ctgaatgcct
     4501 accaggtggc cggcatgtcc gaccgcgcgc tccaggtgta cgaggacgcc gtcgaatccg
     4561 acccggagaa caagctgtac cggtacaact tcggctcgct cctggtggcg gccgagcggt
     4621 acgacgaggc gatcgtgcag ctcgagaagg cgatcgagat cgatccggag tacggcaacg
     4681 cccagtacaa cctgggggca gcctacatca acaaggccgt cgacgtgaac aaccgcatca
     4741 acgagctcga cgacaacctt cgctcgaacc gcgactcgat gtcggagggc gacatccagg
     4801 cggcggaggc cgagatcgac cggctcgccg acgagcgtcg caccctcttc gggtcggcca
     4861 tcgggccgct cgagcgcgcg cgggtgctgt tcgaggccga gggcgagagc gccacggccg
     4921 tctgccaggc cctgtaccag tcgtacgtgc agacgaacca gctcgagctc gcgcagagta
     4981 tcgccgtctg cgccggctac gaggacaact gattcttccg gatccagagc ggggcccggg
     5041 ttcgccgaat ccgggccctt cctgtttttg gcccgaccgc cctccaatcg acgatcccat
     5101 ggccgccgac cgatctgcga ccggtacccg cttcgccacc cttgccgtgc acgccgggca
     5161 ggagcccgac ccgtcgaccg gcgcgatcat gccgccgatc tatcaaacgt cgacctatgc
     5221 gcaggcggcg cccggtgagc acaagggcta cgagtacgcc cgcgtctcga acccgacccg
     5281 cacggcgctc gagggcaacc tcgccgcgct cgagggggcg cggcacggcg tcgccttctc
     5341 gtcgggcgtg gcggcgacgg acgccatcgt gaagtgcctc aggccgggag accacgttgt
     5401 cgcctcgaac gacctgtacg gcgggaccta ccggctcctg cgccaggtgt tcggaccctt
     5461 cgggatcggg tcgagcttcg tggacatgtc gtcctcggag gaggtggagc gggcgttccg
     5521 gcccgagacg cggctgctct ggatcgagac gcccacgaac ccgctcctgc ggatcgtgga
     5581 catcgaggcg ctcgcacgga tgggccgggc gcgcggcgcc gccgtcgcgg tcgacaacac
     5641 gttcgccacc ccgtggctgc agcggccgct cgcgctcggc gccgacctgg tcgtgcactc
     5701 gacgaccaag tacctcggcg ggcactcgga cgtcataggc ggggcggtgt gcacgaacga
     5761 cgacgactgg gccgaccggc tgcgcttcct ggtgaagagc gccggcgcgg tgcccgggcc
     5821 catggactgc ttcctgacgc tgcggggcac gaagaccctg cacctgcgca tggagcgcca
     5881 ctgcgcgaat gcggcgcgcg tggcggactt cctcgcgggc catccggccg tcgggaaggc
     5941 gttctacccg ggcatgccgg accaccccgg gcacgcgatc gcccggcgcc agatgcgcgg
     6001 tttcgggggg atggtctcgt tcatcctcaa ggacgagtcg atcgaggcgg cctttcgcgt
     6061 gctctcgtcg acccgcgtct tcacgctcgc cgaaagcctg ggcggcgtcg agagcctgct
     6121 gaaccatccg gccaccatga cgcacggcag cattccgcgc gaggagcgca tcgcggccgg
     6181 cctgcacgat tccctgatcc ggctctcggt cggcatcgag gacgtggagg acctggtcga
     6241 ggacctggac cgcgcgctgc ggtccgcctg acgctcagta cgcggccagc tcgcggatcg
     6301 ccgcggcgat gtgcgcctcg tcgggcagga cggcgcgctc gagcggatcg gcgaagggga
     6361 ccggcgtgaa ggccccgctg acgcggcgca ccggggcgtc gagcgactcg aaggcgaggt
     6421 cggcgacctg ggcgcagagc tcggcgccga agcccgcgaa gccgttgtcc tcgtacacga
     6481 ccacgacgcg gccggtccgg cggacggact cgagcacggc ttcctggtcc agcggcacga
     6541 tggtgcgcag gtccacgatg tccaccgaga ttccttcctt ctcgaggtgg cgcgcggcga
     6601 cggcggcctt gtgcaccatc atgccgtacg tgatgaccga aacgtcggag ccttcgcgca
     6661 cgcgccgggc cttcccgaag gggagcaggt agtccgcgtc cggctgcggg gagcgcgcgg
     6721 cggccgcgcg gtagagcgcc ttgtgctcca ggaagaggac gggatcctgc atgcggatcg
     6781 ccgtcttgag cagacccttg gcgtcggctg ccgtcgacgg gaaggcgacc acgaggcccg
     6841 gcatgtggcc gaagaacgcc tcgatgttct gcgagtggca gagccccccg tggatgtacc
     6901 ccccgacggg cacgcggatc accatcgggc agctccaggt gtcgttcgag cggtaccgga
     6961 aggacgccac ctggttgcgc agcacggacg cggcgggcca gatgtagtcg ccgaactgga
     7021 tttcgaccac cggcttgtag cccgcggccg agagcccgac ggcggtgccc acgacggacg
     7081 cctcggcgag cggcgagttg aagcagcggt cctcgccgaa gcgcttcgtg aggttgcgcg
     7141 tcgctgtgaa cacgccgccc ttgtcgccgc ccacgtcctc gccgtagacc aggatgcgct
     7201 cgtcgcgctc catctcctcg tgcagggcat ggttgatggc gtcgaccatg acgatcggct
     7261 cgccgtccgg ctcggacgcc tcgtactcga ggccgaggtc tccctcgaac aggacgtgcc
     7321 gggtggcggt cgacgggtcg ggatcggcct gggtcatggc ccacgcggcc gcctggtcca
     7381 cctcggtctg cacttccttc cggatccgcg tgatcgccgg ttcgtcgagc acgccggcgg
     7441 ccacgagccg ctcctcgaac cggacgatgg gatcgacctg gcggtcggcc tcgagctcct
     7501 cgctggagcg gtacttggcg tgattgtccg aggaggagtg ggggaggagc cggaccaggt
     7561 ccgccaccag gcacacgggc cctccgcccg agcgcatcga ctcgatggcc gcccgggccg
     7621 ccgaccaggt ttcgaagaaa tcggtgccgt cgacgctcgc tcgccggagc ccctcgtatc
     7681 cgcgggccag cttgtaggcg ctcccgccgg cggtctggtc ctggaccggg accgagatcg
     7741 cgtacttgtt gtcctggacc aggaagagca cggggatgcc ggcccgggcg gcccagttca
     7801 tggcctcgtg gaattcgccc tgggacgtgg ccccgtcccc acacgagcag tacacgtagg
     7861 cgtcgaggcc gggatcgcgc cggatggcga gccccacgcc cacggcgggc aggaactgcg
     7921 agccgaccga cgacgacagg gggaggatgt ggcgctcccg gttcgagtag tgctccgaca
     7981 tctggcgccc gccggaattc gggtcgtcgg ccttggccag gtggtgcagc atgacctcac
     8041 gggacgttcc gcccagcatg agatacatcg tgaggtcgcg gtagtacccg ctgaaccagt
     8101 cgtgcccggg ccggctgagc aggccgaccg ccgcctgggc cgcctcgtgg cccgcgcaac
     8161 cgatgtggaa gaagcccttc ccctgcttca ggagcgtgag catcttctcg tcgagccgac
     8221 gcgacgtgag catcgtgcgg tacacgccca ggagcgtttc cggctcgaac gcctccgggc
     8281 cgacgggcgt gaagtccagc tcgtcgccga tcggggcgtc gtgacggtcg gatccgtcgg
     8341 ctaccgcctg gccggaccgc ccgttgccgg
//
LOCUS       DBNHQZ010000016         4804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1357649, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000016 DBNHQZ010000000
VERSION     DBNHQZ010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1357649"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="AC16YU_00405"
     CDS             <1..944
                     /locus_tag="AC16YU_00405"
                     /inference="COORDINATES: protein motif:HMM:NF022525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIR6063710.1"
                     /translation="VAKTIPETEASIPRQLGILRLPAAPDVPVGSHCDDPYECPFKAH
                     CWRDVPKNSVFTVPRLSGTLKDEYWRAGVCHVLDIPDVHTLNPPQQRYVECVRRQEPR
                     IMALGIAHELGKLRHPIHFLDFETDALPIQRFHGLRPYEKFPFQYSCHVEHADGTIEH
                     REYLHDDLSDPRPELARRLAGDVADEGSIVAWNAGFERGVLEQLAGLVRDRRLKGMAD
                     RLWDLLPVVRANIDHPDFEGSYSIKKVLPVLVPGLTYEGRAVTDGGDAQAAWDQAIRL
                     EDGPEKDALLRDVRAYCELDTLAMVEILKVLRGYATT"
     gene            complement(978..1628)
                     /locus_tag="AC16YU_00410"
     CDS             complement(978..1628)
                     /locus_tag="AC16YU_00410"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR6063711.1"
                     /translation="MPSLRVEGFTVSIDGFGAGPGQSLEQPLGIGGEALHGWLVATET
                     FQRMMGEDSGTTGIDERYARRGFDGVGAWIMGRNMFGPIRGPWPDEEWKGWWGDDPPY
                     HGPVFVLTHHRREPVEMLGGTVFHFVTGGIREAFDRAFEAAGGLDVRLGGGVSTVRQY
                     LSWGLVDEMHLAVSPVLLGRGEALFAGLDLAGLGYEVAGSEASEEAVHVVIRKAGS"
     gene            1951..2595
                     /locus_tag="AC16YU_00415"
     CDS             1951..2595
                     /locus_tag="AC16YU_00415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6063712.1"
                     /translation="MACAHCVDAGRVFNDRTARWEAIRYRWFGPSRPTKHLLRAVKGR
                     IGDAGTLLDVGGGVGTIPRHLSDRLDSCTAVDASPAYLSVARSEAERYGFGDRMTWVE
                     GDFLDVRDRVPPHDAVTMDRVICCYPDMESLVRATSGLARRVYVLVFPREGLLNRVAF
                     PLMNLLLRIRGSAFRIFLHPTRRVDDVAASGGLALSEVRRTLLWQVRVYERPSP"
     gene            complement(2653..3531)
                     /locus_tag="AC16YU_00420"
     CDS             complement(2653..3531)
                     /locus_tag="AC16YU_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063713.1"
                     /translation="MKRASVSFVATLLFIAGCAPQPSVDVEALQSEIAELRAHAGPPP
                     ASLDQFYPPQAEAPVYRIKMHEVGGPLSGMVVDLMQGDMENVPGQFEAFRAAYQEVAA
                     MVPEWQDLWPMTPVDELGTALQGGDQGAVMGAVQGVGAVCHSCHVDAMPKVHQRYAWP
                     SFADVRATDPVSGDEVPMAGYMRAMETSFSGMMNDLQQGQVDRAREHYRAFYDRFGGL
                     ADLCIECHDTEREYFIDEEVQRVVRDVGTAIEADPPDAARIGGLAQRIGEEACFRCHL
                     VHVPAAFAQAGWSTAH"
     gene            3706..>4804
                     /locus_tag="AC16YU_00425"
     CDS             3706..>4804
                     /locus_tag="AC16YU_00425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6063714.1"
                     /translation="MNRLLLFAAVMGLVPSLAASAQVTPRFTDPERAEKVRATAATVE
                     AMYRDYVEGRHMPGFVYGVVLDGELLYSGGFGYSNLEEQIPVSPMSLFRIASMSKSFT
                     ALAILQLRDAGKLSLDDPASKYIPEMRQLKYLTSDAPEITIRHLLTHGAGFPEDNPWG
                     DRQLADSDEELVRLIEEGVSFSNVPGVAYEYSNLGFALLGQIVQRVSGMEFQAYSREH
                     IFGPLGMDATVWEYTDAPPDRLAPGYDWIDDAYVRIPLEHHGSFGAMGGLITSIEDFT
                     RYVALHLSAWPPRDGEDSGPLTRSSLREMHHPWRFASLGARTGGRDCASVRAYAYGLA
                     WSRDCDGKVTVGHSGGLPGFGSNWTILPDYGL"
BASE COUNT          757 a         1720 c         1575 g          752 t
ORIGIN      
        1 aggtcgcgaa gaccatcccc gaaacggagg cctcgatccc gcggcagctc gggatcctgc
       61 ggctgcccgc ggcgccagac gtccccgtcg gctcccactg cgacgacccg tacgagtgcc
      121 cgttcaaggc gcactgctgg cgggacgtgc cgaagaactc ggtgttcacg gtcccgcggc
      181 tctcgggcac gctcaaggac gagtattggc gggccggcgt gtgccacgtg ctggacatcc
      241 cggacgtcca cacgctgaat ccgccgcagc agcgatacgt cgagtgcgtc cgccggcagg
      301 agccgcggat catggccctg ggcatcgcgc acgagctggg gaagctccgg cacccgatcc
      361 acttcctcga cttcgagacc gacgcgcttc ccatccagcg cttccacggc ctccggccgt
      421 acgagaagtt ccccttccag tacagctgcc acgtcgagca cgccgacggc acgatcgagc
      481 accgggagta cctgcacgac gacctttccg acccgcgacc cgagctcgcc cgccggctcg
      541 ctggagacgt cgcggacgag ggatccatcg tcgcgtggaa tgccggcttc gagcgcggcg
      601 tgctcgagca gctcgccggc ctggtccgcg accggcgcct caagggcatg gcggaccgcc
      661 tctgggacct cctgcccgtc gttcgcgcca acatcgacca tcccgacttc gagggatcct
      721 actccatcaa gaaggtgctt cccgtgctgg tccccgggct cacgtacgag gggcgggcgg
      781 tcacggacgg gggcgacgcg caggccgcgt gggaccaggc gatccgcctc gaggacggac
      841 cggagaagga cgccctgttg cgcgacgtcc gcgcctactg cgagctggac acgctcgcga
      901 tggtcgagat cctgaaagtg ctgcggggct acgcgacgac ctgaaccgcg tcagcccccg
      961 aacctcgcgg cccgcgatca ggaccccgcc ttcctgatca cgacgtgaac ggcctcctcc
     1021 gaagcctcgg acccggccac ttcgtacccc aacccggcaa gatcgagccc ggcgaacagc
     1081 gcctccccac ggccgagcag caccgggctc acggccaggt gcatctcgtc gaccagcccc
     1141 cacgagagat actggcgcac cgtggagacg ccgccaccga gccggacatc gagcccgcct
     1201 gcggcctcga aggcgcggtc gaacgcctcc cggatcccgc ccgtcacgaa gtggaagacg
     1261 gtgccaccca acatctcgac gggctcccgc cggtggtgcg tgagcacgaa gacgggaccg
     1321 tggtacggcg ggtcgtcgcc ccaccagcct ttccattcct cgtcgggcca ggggccgcga
     1381 atcgggccga acatgttgcg ccccatgatc catgctccga cgccgtcaaa gccgcgtcgg
     1441 gcgtagcgtt cgtcgatccc ggtcgtaccc gaatcctctc ccatcatgcg ctggaaggtc
     1501 tcggtcgcaa ccagccaccc gtggagcgcc tccccgccga tacccagcgg ctgctcgagg
     1561 ctttgaccgg gaccggctcc gaagccgtcg atcgacacgg tgaatccttc aacacgaaga
     1621 ctgggcatgg ccggcgcgcg aatgcgaggt agctggcgtt cacagacccg cactcgcccg
     1681 aaaaggtccg ctcccctgtc gaggtgacgc gctgccgagg gcttggatct gcaacttccc
     1741 ccgaccttcc acgcgtcagc gccgtgttcc tggttcgccc ggcaccgacg acgtcacgga
     1801 gccggcgctg cgcgaggcgg ccatcatggc gctcgccgag gcggcggagg acaatcagta
     1861 gtccgtcttg cacgaacgcg gcggcgcgag cgggaagtga accctgaccc cgggcccgcg
     1921 tagcacggcg gtcaatccgt tcacgatccg atggcctgcg cccactgcgt cgatgccggt
     1981 cgcgttttca atgaccggac cgcgcgctgg gaagccatcc ggtaccggtg gttcgggcct
     2041 tcgcgcccca cgaagcacct gcttcgcgcc gtgaagggac gaatcggcga cgccggaacg
     2101 ctgctcgacg tgggcggagg cgtcgggacc atcccacggc atctctccga ccggctggat
     2161 tcgtgcacgg ccgtcgacgc ctcgcccgcc tatctctcgg tcgcccgctc cgaggccgag
     2221 cggtacgggt tcggggaccg catgacctgg gtcgaaggtg atttcctgga cgtccgcgac
     2281 cgggtgcccc cgcatgacgc ggtgaccatg gaccgggtca tctgctgcta tccggacatg
     2341 gagtcgctgg ttcgggcgac gtccggcctg gcgcggcgcg tgtacgtcct ggtcttcccc
     2401 agggagggcc tgctgaaccg ggtcgcgttc ccgctcatga acctgctcct tcggatccgg
     2461 ggatcagcgt tcaggatctt cctgcacccg acacgacgtg tggacgacgt ggccgcctcc
     2521 gggggtctgg ccctgtcgga ggtgcgccgc accctgctgt ggcaggtgcg cgtttacgag
     2581 cgtccgtcgc cctagaacga cggggcgatt cccggcccga cggctgcgcc tcccggcgga
     2641 ttcatcgacg atctagtgcg ccgtgctcca tcccgcctgc gcaaacgccg ccggcacgtg
     2701 caccaggtgg catcggaagc aggcttcttc gccgatccgc tgcgccagtc cgccgatccg
     2761 cgcggcgtcc ggtggatcgg cctcgatcgc ggtgccgaca tccctcacga cgcgctgcac
     2821 ctcctcgtcg atgaagtact cgcgctcggt gtcgtggcac tcgatgcaga ggtcggccag
     2881 gccgccgaac cggtcgtaga acgcgcggta atgctcccgc gcccggtcta cctggccctg
     2941 ctggagatcg ttcatcattc cgctgaacga cgtctccatc gcacgcatgt acccggccat
     3001 cgggacctcg tcgcccgaga cggggtcggt agcccgaacg tccgcgaacg acggccaagc
     3061 gtaacgctgg tgcaccttgg gcatggcgtc cacgtggcag ctgtggcaca cggcgcccac
     3121 gccctgcacg gctcccatca cggcgccctg gtctcccccc tggagggcag tcccgagctc
     3181 gtcgaccggg gtcatgggcc agagatcctg ccactcgggc accatcgcgg cgacctcctg
     3241 gtaggcggcc cggaacgcct cgaactggcc gggcacgttc tccatgtctc cctgcatcag
     3301 gtcgacgacc atcccgctga gcggcccccc gacctcgtgc atcttgatcc ggtagaccgg
     3361 cgcttcggcc tgcggcggat agaactggtc gagggacgcc ggcggcgggc ccgcgtgagc
     3421 ccgtagctcg gcgatttcgc tctggagcgc ctcgacgtcg acggacggct ggggcgcgca
     3481 cccggcaatg aagagaaggg tggccacgaa ggagactgat gcacgtttca tggttcgtta
     3541 cctggaggta ggtgaagaca gcccggcgca caggtcgaga gcggccgacc gtccggcgtt
     3601 gcagcacacg ggaatgaacc acttcggcat gcaccagtca acaggatggc gtatccggcc
     3661 gctgatgtat accgtgtggg tcccgtccac agccgatcct ccgccatgaa ccggctcctg
     3721 ctcttcgccg ccgtcatggg cctcgttccc tccctcgccg cctccgccca ggtcaccccg
     3781 cggttcaccg atccggagcg ggccgaaaag gtccgggcca cggcggccac ggtcgaagcg
     3841 atgtacaggg actacgtcga aggccggcac atgcccggat tcgtgtacgg ggtggtgctg
     3901 gacggcgagc tcctctactc cggagggttc gggtattcca acctcgagga acagattccc
     3961 gtgagcccga tgtcgctctt ccgcatcgcc tcgatgagca agagcttcac ggcgctggcc
     4021 atccttcagc tccgcgacgc ggggaagctg agcctcgacg atcccgcgtc gaagtacatc
     4081 cccgagatgc ggcagctgaa gtatctcact tccgacgccc cggagatcac gatccgccac
     4141 ctcctcacgc acggagccgg attcccggag gacaacccgt ggggcgaccg gcagctggcg
     4201 gactcggacg aggagctggt gcgcctcatc gaggaaggcg tctcgttctc gaatgtcccg
     4261 ggcgtggcgt acgaatacag caacctcggt ttcgcgctcc tcggacagat cgtccagcgg
     4321 gtctcgggaa tggagttcca ggcatacagc cgggaacaca ttttcgggcc cctcggcatg
     4381 gacgctacgg tgtgggagta cacggacgcg ccgccggatc gcctggcgcc cggctacgac
     4441 tggatcgacg acgcgtacgt ccgcatcccg ctcgagcacc acgggtcctt cggggcgatg
     4501 ggcgggctca tcacgtccat cgaggacttt acgaggtacg tggccctgca cctgtccgcc
     4561 tggcctcccc gcgacggcga ggattcggga ccgctgacgc ggagctcgct gcgcgagatg
     4621 caccatccct ggcggttcgc ctcgctcggc gcccgcaccg gcggcaggga ttgcgcctcg
     4681 gtccgggcgt atgcctatgg cctggcctgg agccgggact gcgacggcaa ggtgaccgtc
     4741 ggccacagcg gcgggctgcc cggattcgga agcaactgga ccattcttcc ggactacggc
     4801 ctgg
//
LOCUS       DBNHQZ010000017         3961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_951615, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000017 DBNHQZ010000000
VERSION     DBNHQZ010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_951615"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16YU_00430"
     CDS             <1..719
                     /locus_tag="AC16YU_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6063715.1"
                     /translation="TWTESFRNADPDAFFDCFSFWDRERGFAFSDSHDGEFTLVRTTD
                     GGRSWHRIDPAAVPDARAGEGAFAASGTCVATRAGGLGWFATGASGVDTRVIRTLDYG
                     DTWTESVTPIASRGGTEGISSVAFADDVRGAVFGGDFTRPDSVHANLAMTRDGGATWA
                     SAGPVPLGGAVFAGSAVPGTGGPTWLAVAPTGSALSTDDGATWTRIDDTDWWTLSAAG
                     PDAVWAAGPGRISRLVDRAR"
     gene            911..2365
                     /locus_tag="AC16YU_00435"
     CDS             911..2365
                     /locus_tag="AC16YU_00435"
                     /inference="COORDINATES: protein motif:HMM:NF021390.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG3650 family protein"
                     /protein_id="HIR6063716.1"
                     /translation="MPNPRFLRSAPVAALVLACACAPTDGPVPPPGAPAAEREGRAYV
                     YDCADAGLRFTAWVAPDSMDVWLPAALGRPPVRIPRVEAASGARYEGEGVEVWTGERE
                     ATLRIDGTDHSPCELDSQASTWEDARLRGVSFRGVGNEPGWLVEIRADGSARLVLDYG
                     ERELVISAADAVREESDLQTVYRAVTDSHEVEMTIEAEWCPDTMSDETFDHTVAVTVG
                     DRSYRGCGRWLGVDAPGLGPRARRAFDPARDLLALHYDHAPDRDDGQSAVADRVMLET
                     ELGAAWIARSALAVSGAYGENAADFDAGSDAVMDAVFGDVGGWIAAHGDRSAAMDRMT
                     DRWLATLRAGGDVWVKEGGQSDLTADLVARVQDALPDVDTRARIHVVQHSDWNEDMTT
                     DAALAFVREHTDYVRIRDANAYLNLEGGDRAFEAAALAHDRFGAMWASAFSYYPPAER
                     LDFSDTGELMHILGVDEMGIDAFRARYLDPDRND"
     gene            2628..>3961
                     /locus_tag="AC16YU_00440"
     CDS             2628..>3961
                     /locus_tag="AC16YU_00440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6063717.1"
                     /translation="MLEELGRGGMGVVYVARDVRLGRRVALKFLTVELDADSTARGRF
                     VQEAQAASALNHPNICTIHEIGESDDGTIFIAMALCEGRTVRERIADGTLTARDAHRI
                     GRDLAAGLAAAHRIGIVHRDVKPANIIVSDDGHVRILDFGLAKVANASLTRTGATVGT
                     ADYMSPEQARGEPVDARSDVWSAGCVLYEMIAGVAPFEAAHDTAVVYRLMNEEPEPLR
                     PHLPPALVALEDIVLRCLRKDPDARYPSAAELQADLARLDDVGPPRPARRALRYGLAG
                     GGVVVVALAAVLLARGGAPTAGGIAVLPFRATGMDSLECVALSELVSQELGDAVHAAS
                     SGFWVFPQSEVRARHIRSGSDAARELGAGIGVEGYCMAVGDNVELDLSVVDAAAMRQV
                     GEARVSVDRVDLRGIQSAAATALADLLPGFPRRSGARADDVGGTRLPAAFTH"
BASE COUNT          553 a         1389 c         1441 g          578 t
ORIGIN      
        1 cgacgtggac ggaatcgttc cgcaacgcgg accccgacgc cttcttcgac tgcttctcgt
       61 tctgggaccg ggagcggggc ttcgcgttca gcgacagcca cgacggggag ttcaccctcg
      121 ttcgcacgac ggacggcgga cgatcctggc accggatcga tcccgccgcc gttccggacg
      181 cccgggcggg cgagggcgca ttcgccgcga gcggcacctg cgtcgcgacg cgggcgggcg
      241 gcctcggctg gttcgcgacc ggcgcgtcgg gcgtcgacac gcgggtgatc cgcacgctgg
      301 attacggcga tacgtggacg gaatccgtca cgccgatcgc gagccgcgga ggcacggagg
      361 ggatcagttc cgtggccttc gccgacgacg tccggggcgc cgtgttcggc ggcgacttca
      421 cgcgccccga ctccgtacac gcgaacctcg cgatgacccg cgacggaggc gccacctggg
      481 cgagcgccgg tcccgtgccg ctcggcggcg cggtgttcgc cggatcggcc gtccccggca
      541 cggggggccc cacctggctc gccgtcgccc ccacggggtc ggccctttcc acggacgacg
      601 gcgcgacgtg gacgaggatc gacgatacgg actggtggac cctctcggcc gcgggcccgg
      661 acgcggtatg ggcggccgga cccgggcgga tcagccggct cgtcgaccgc gcgcgctgag
      721 cgccaccccg gccccgtcga gaattcgccg cgcacgcgat cgctcacgtc ggtcggtcgc
      781 ccggtggccg gatcgacggg acaccacgcg gggagcgccc gaacccacct ccgatccacg
      841 catcgtgtaa gcgggaatct gatcggtccc cggaggtata ctggagcccc gaccttccga
      901 gagccctgcg atgccgaacc ctcgattcct ccgctccgcc ccggtcgccg ccctggtcct
      961 cgcctgcgcc tgcgcgccga cggacggccc ggtcccgcct cctggcgccc cggccgccga
     1021 gcgcgaggga cgcgcctacg tctacgactg cgcggacgcc ggcctccggt tcacggcatg
     1081 ggtcgcgccc gactcgatgg acgtctggct gccggccgct ctcgggcgcc cgccggtccg
     1141 gatccctcgc gtcgaggcgg cgagcggcgc ccgctacgag ggcgagggcg tcgaggtctg
     1201 gaccggagag cgcgaggcga cgcttaggat cgacggtacg gaccattccc cgtgcgagct
     1261 cgattcgcag gcatcgacct gggaggacgc caggcttcgg ggcgtttcgt tccggggcgt
     1321 ggggaacgag ccgggctggc tcgtcgaaat ccgggctgac ggaagcgcca ggctggtgct
     1381 cgactacggg gaacgggagc tcgtgatctc ggctgcggac gccgttcggg aggaatcgga
     1441 cctgcagacg gtctaccggg cggtcaccga ctcgcacgag gtggagatga cgatcgaggc
     1501 ggagtggtgc ccggacacga tgagtgacga gacgttcgat cacacggtgg cggtgacggt
     1561 cggcgaccgg tcatatcgag gctgcggccg gtggctcggt gtggacgccc ccggccttgg
     1621 cccgcgcgcg cgacgggcgt tcgaccccgc gagagacctg ctcgcgctgc actacgatca
     1681 cgccccggac cgggacgacg gccagagcgc cgtcgccgac cgcgtgatgc tcgaaacgga
     1741 gctcggcgcg gcctggatcg cccgcagcgc gctcgccgtg tccggagcgt acggcgagaa
     1801 cgcggccgac ttcgacgccg gttccgacgc ggtgatggat gccgtgttcg gcgacgtcgg
     1861 aggctggatc gccgcccacg gcgatcgctc cgcggccatg gaccggatga cggaccgctg
     1921 gctcgcgacc ctgcgcgcgg gcggagacgt ctgggtcaag gagggcggac agtcggacct
     1981 cacggccgac ctcgtggccc gcgtccagga cgccctcccg gacgtggaca cgcgtgcgcg
     2041 catccacgtc gtgcagcaca gcgactggaa cgaggacatg acgacggacg ccgcgctggc
     2101 cttcgtccgc gagcacaccg attacgtccg gatccgcgat gcgaacgcct acctgaacct
     2161 cgagggcggc gaccgggcgt tcgaggcggc cgccctggcg cacgaccggt tcggcgcgat
     2221 gtgggcgtcg gccttctcct attacccccc ggccgaacgc ctggacttct cggacaccgg
     2281 ggagctcatg cacatcctgg gggtcgacga gatgggcatc gacgcgttcc gggcacggta
     2341 cctggatccg gacaggaatg attgatagca ctattgttat ataacaataa tattattttc
     2401 actattccag cctcgcctgc gagcgcagtc cggccccgat cgtgcggagg cggccggcgg
     2461 gcaccccaga ttgcacttcc ttcggcgacc cgttagcttg ccgaaacgca cagcggcggg
     2521 tgccccctcc ggccggctgg agaggtcgtg gcagtcgggc cggccggagt gcgcagcgtt
     2581 cgcgaggtga cgaacccctg atcggcagac tcatatcgca tttcgaggtg ctcgaggagc
     2641 tcggtcgggg cggcatgggg gtcgtgtacg tggcccggga tgtccgcctc gggcggcgcg
     2701 tggccctgaa gttcctcacg gtcgaactcg atgccgactc gacggcgcgc gggcgtttcg
     2761 tgcaggaggc ccaggcagcg tccgcgctga accaccccaa catctgcacg atccacgaga
     2821 tcggcgagtc ggacgacgga acgatcttca tcgcgatggc cctctgcgaa ggccggaccg
     2881 tgagggagcg tatcgccgac ggcacgctga ccgcgcgtga cgcccatcgg atcggccggg
     2941 acctggccgc cgggctggcc gcagcgcacc gcatcggcat cgtgcaccgg gacgtcaagc
     3001 ccgcgaacat catcgtgtcg gacgacgggc acgtccgcat tctcgatttc ggcctggcca
     3061 aggtcgcgaa tgccagcctc acgcggaccg gtgcgaccgt cggcaccgcg gactacatga
     3121 gccccgagca ggcccgcggc gagccggtcg atgcgcgatc cgacgtctgg tcggccggct
     3181 gcgtgctgta cgagatgatc gcgggcgtcg ccccgttcga ggcggcgcat gacacggcgg
     3241 tggtttaccg cctcatgaac gaggagccgg agccgcttcg tccccatctt ccgccggccc
     3301 tcgtcgcact cgaagacatc gtcctgcggt gcctgcggaa ggatcccgat gcccgctatc
     3361 cctcggccgc cgagctccag gcggacctcg cgcgactcga tgacgtaggc ccgccgcggc
     3421 cggcgcggag ggcactccgg tacggcctgg ccggcggcgg ggtggtcgtc gtcgccctgg
     3481 cggccgtgct gcttgcccgc ggcggtgcgc cgaccgcggg cggcatcgcg gtgctgccgt
     3541 tccgcgccac gggaatggat tcgctcgagt gcgtcgcgct gtccgaactc gtatcgcagg
     3601 aactcggcga cgcggtgcac gcggcctcct ccggtttctg ggtgttcccc cagagcgagg
     3661 tccgggcacg gcacatccgc tcgggctcgg atgcggccag ggagctcggc gccggaatcg
     3721 gcgtcgaggg atactgcatg gcggtgggcg acaacgtgga actggacctc tccgtcgtcg
     3781 atgcagccgc gatgcgccag gtgggcgagg cccgcgtctc ggtggaccgg gtcgatctcc
     3841 ggggcatcca gtcggctgcg gcgacggcgt tggccgacct gctgcccggc tttccgcggc
     3901 gctccggcgc gcgagccgac gacgtcggcg gcacccgatt accggccgcg ttcacccatt
     3961 t
//
LOCUS       DBNHQZ010000018         2923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2983784, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000018 DBNHQZ010000000
VERSION     DBNHQZ010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2983784"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="AC16YU_00445"
     CDS             <1..1514
                     /locus_tag="AC16YU_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HIR6063718.1"
                     /translation="RLSLLFGYLFHVAAFIGNLYALHLGRDDRATIQHTSAMLYAGSA
                     LGAVFAGDLITLFVFWELLALTSVFLVWARGTERSYRAGFRYLVIHVVSGVILLSAAL
                     LRAHETGSIALEHVGLDGSLGAWLLLIAFGIKAAFPLAHNWLTDAYPEGTVTGAVFLS
                     AFTTKVAVYALARTFAGTEVLIYVGAVMTAFPIFFAVIENDLRRVLGYSMINQIGFMV
                     TGVGIGTALAVNGAVAHAFNDVIFKGLLFMSMGAVLHQTGRINGSDLGGLYKSMPWTA
                     GFCIVGASSISAFPLFSGFVSKSMVMSAAIEGGHPVVWLVLLFAAAGVFHHAGIKIPF
                     FAFYGHDSGIRTTEAPRNMLFAMGLASILCIVIGTFPQQTLYRILPFDAPYNPYDATH
                     VLTQIQLLFFGALAFVGLQKFKIYPAELRSTNIDVDWTYRWLAPRFVGFVGRVVMRVD
                     GAIRRAVLSALGGTVESLGRTHGRMGPLARSWPTGSMVLWVAIMLAASLIVYL"
     gene            1578..2393
                     /locus_tag="AC16YU_00450"
     CDS             1578..2393
                     /locus_tag="AC16YU_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063719.1"
                     /translation="MNVRLAAADPFRSQAERLGGRLTSLGFVVTVVKPAEDAELRLES
                     AEGLPELLPSRSAAIIGLAEPRDALVGSPEGAASLSELPATARVAVSGTLRAEMLGVH
                     RPELPVVRVDDPRDAADLLDRGDVDAWIAPVGVARAVGLGDAVREIFEPTSWATAVGR
                     GSVVLQAEAPGKVLTELLAELDEPAARAALRAEQVVLEVLGVGSDATVGVMARPHGPL
                     LRMRALLPARADRRLVRADFSGRLEDPVTAGRRIAEQLIERGGLELLATAASA"
     gene            2502..2918
                     /gene="rfaE2"
                     /locus_tag="AC16YU_00455"
                     /pseudo
     CDS             2502..2918
                     /gene="rfaE2"
                     /locus_tag="AC16YU_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
BASE COUNT          385 a          965 c         1037 g          536 t
ORIGIN      
        1 atcggctgag cctgctgttc ggctacctct tccacgtcgc ggccttcatc ggcaacctct
       61 acgcgttgca cctcggacgc gacgaccgcg ccaccatcca gcacacgtcg gcgatgctct
      121 acgccggcag cgcgctcggc gcggtattcg cgggtgatct gatcacgctg ttcgtgttct
      181 gggagctact ggcactcacg tccgtattcc tcgtgtgggc gcgcggaacg gaacggtcct
      241 atcgggcggg cttccggtac ctcgtcatcc acgtggtctc aggcgtcatc ctgctttccg
      301 ccgcgctcct ccgggcgcac gagacgggtt ctatcgcgct cgagcacgtg ggcctggacg
      361 ggtcactcgg ggcgtggctc ctgctgatcg ccttcggcat caaggccgcg ttccctctgg
      421 cgcacaactg gctcaccgac gcctatcccg aaggtacggt gaccggcgcg gtcttcctga
      481 gcgcgttcac gacgaaagtg gcggtgtacg ccctggccag gacgttcgcg gggaccgagg
      541 tgctcattta cgtcggggcg gtcatgacgg cgttcccgat cttcttcgcc gtgatcgaga
      601 atgacctgcg ccgcgtgctc ggctacagca tgatcaacca gatcgggttc atggtgaccg
      661 gggtcggcat cgggaccgcc ctcgcggtca acggcgccgt ggctcacgca ttcaacgatg
      721 tgatcttcaa gggcctgctc ttcatgtcca tgggcgccgt gctccatcag acgggacgca
      781 tcaatggctc ggacctcggc ggtctgtaca agtcgatgcc gtggaccgcg ggcttctgca
      841 tcgtgggggc gtcgtccatc tcggcgtttc cgctgttcag cggattcgtt tccaagtcca
      901 tggtgatgtc ggccgcgatc gagggaggcc atccggtggt ctggctcgtc ctcctgttcg
      961 cggcggcggg tgtgttccat catgcgggca tcaagattcc gttcttcgcg ttctacggtc
     1021 acgactcggg catccgcacg acggaggccc cgcgaaacat gcttttcgcc atggggttgg
     1081 cgtcgattct ctgcatcgtg atcgggacgt tcccccagca gacgctctac cggatcctgc
     1141 cgttcgacgc gccctacaat ccttacgacg cgacgcatgt cttgactcag atccagctcc
     1201 tgttcttcgg tgcgctcgcg ttcgtcgggc tgcagaagtt caagatctat ccggccgaac
     1261 tgcgctccac caatatcgat gtcgactgga cgtatcggtg gctggcgccg cggttcgtgg
     1321 gcttcgtcgg gcgcgtggtg atgcgcgtgg acggtgcgat ccgccgtgcg gtgttgtcgg
     1381 ccctgggcgg gaccgtggag tcgttaggga ggacgcacgg tcgaatgggc ccactggcgc
     1441 gctcgtggcc taccggtagc atggtgctct gggtagcgat catgctcgcg gcgagcctga
     1501 tcgtctatct gtaaccgggc gcggcgcccg aacgcccctc ctgaggcggg cgaccggaaa
     1561 cggcgtccgg atcgagcgtg aacgtacgac tcgcggctgc ggacccgttc cgtagccagg
     1621 ccgagcggct cgggggccga ctcaccagcc tgggcttcgt ggtgaccgtg gtgaagcccg
     1681 ctgaagatgc tgagctgcgc ctcgagtccg cagaagggct ccccgaactg ctgccgtcgc
     1741 gctcagcggc gatcatcggt ctggccgagc cgcgcgacgc gctcgtgggc agccccgaag
     1801 gagcggcgag cttgtcggaa ctcccggcca cggcccgggt ggcggtctcg ggcacccttc
     1861 gagcggagat gctcggcgta caccgcccgg agctccccgt ggtacgggtc gacgacccgc
     1921 gcgacgccgc agatctcctc gaccgcgggg acgtcgatgc gtggatcgcg ccggtgggcg
     1981 tggcgcgcgc ggtcgggctc ggtgacgccg tccgggagat cttcgagccg acatcctggg
     2041 cgacggccgt ggggcggggt agcgtcgtcc tccaggccga ggcccctggt aaagtcctga
     2101 ccgagcttct cgccgagctg gacgagccgg ccgcccgcgc ggcgctgcgg gccgagcagg
     2161 tggtcctcga ggtgctcggc gtcggttccg acgctacggt cggcgtcatg gcgcggccgc
     2221 acggtccgct ccttcggatg cgtgcgctgc tgcccgcccg ggcggaccga cggctcgttc
     2281 gtgccgactt ctcgggacgg ctcgaggacc cggtgacggc ggggcgtcgc atcgccgagc
     2341 agctgatcga gcgaggcggc ctcgagctgc tcgcgaccgc ggcctccgcg tgagggcgtg
     2401 ggcgtgacgg accgggcgac gtgcgcggaa tgaggacccc tgcggacaag gtgctgtcga
     2461 gggacgcgtt cctggccggg ttcggccgcc cgcgctccgg gcgattggtc ttcacgaacg
     2521 ggtgcttcga tctcctgcac cccgggcacg tgacctatct ggcccgggcc cgcgcgctcg
     2581 gagacgccct ggtggtcggc gtgaacaccg acgcgtcggt tcgccggctc gagaaggccg
     2641 cgaaccgccc gctcgtggcc gaaggcgccc gcgcgctcgt gttggccgcc ctcgagtcgg
     2701 tggacgcggt gtgtctcttc gacgaggata cacccgccgc gctcatcgag gcgctcctgc
     2761 ccgacgtgct cgtgaagggc ggagactacg tcgtcgacga ggtcgtcggc cgcgaggcgg
     2821 tcgaaggggc cggggggcgc gtggagatca ttccgttcgt ggagggctac tccacgacgg
     2881 cactgctgcg acgaatcagg gagactgggg atggctgacc gcc
//
LOCUS       DBNHQZ010000019         2434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1358799, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000019 DBNHQZ010000000
VERSION     DBNHQZ010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1358799"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..777)
                     /locus_tag="AC16YU_00460"
     CDS             complement(10..777)
                     /locus_tag="AC16YU_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063720.1"
                     /translation="MKAKTLAAVLTVAAATVAAVLPGPSPTVYRTCATVLDAEVCTWL
                     TMDGDRAVELGATVPLALIEAVPADAEMVWPPEQLGVVELPAEAKKALGIDHMGLNWE
                     AHGHPPATFLAPHFDFHFYSIPQESVHAIDCADLSKPSALPAGYTLPDIEIPGMGNLV
                     GLCVPRMGMHAMPAADAEATESFDASMILGYYGGEPIFFEPMVSRELLLERSDFTLAV
                     PSVEGLPEGVRYPSEFRAEYDADAEQYRLVFSGFETR"
     gene            complement(1050..2027)
                     /locus_tag="AC16YU_00465"
     CDS             complement(1050..2027)
                     /locus_tag="AC16YU_00465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6063721.1"
                     /translation="MTSCPDGWLARQNLRPYAAWRPERQVPGPVTASISREATMILVV
                     GATGSLGQKIVRGLLDRSEVVHALARPSSNWPPLEAAGARVVFGDLKQPASLERACEG
                     ADAVITTASISKTGDDSVENVDLQGNRNLVDSARASGVGHLIFVSTVGASVDSPVPVF
                     RAKGETEERLRESGIAHTILQPDPFMDVWFGMLIERPMHAGEPVTLVGESLRRHAFVA
                     EKDVAAFAVASTRTAAARNATLVIGGPEAVSLTEVVRAYEQASGRPIPIRSVAPGDPI
                     PGIPEPVWGIAAALESYDSVIPMQDTAETFGVTLTSVRDFARSRVAGTD"
     gene            complement(2061..2264)
                     /locus_tag="AC16YU_00470"
     CDS             complement(2061..2264)
                     /locus_tag="AC16YU_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR6063722.1"
                     /translation="MSVARVTEIKSASKTSFDDALRVGIERASETLQNVKGAWIAEQE
                     VIVENGKIAEYRVLLKVTFILKN"
BASE COUNT          417 a          799 c          814 g          404 t
ORIGIN      
        1 gcccgttcct tatcgtgttt cgaagcccga gaagaccagc cggtactgct cggcgtctgc
       61 atcgtactcc gcgcggaact cgctcggata gcggacgccc tccggcaggc cctcgaccga
      121 cggaacggcg agggtgaagt ccgaccgctc gaggagcagc tcgcgcgata ccatcggctc
      181 gaagaaaatc ggctcgccgc cgtaatagcc gaggatcatg ctggcatcga aggactcggt
      241 ggcctcggca tcggcagccg gcatggcatg catgcccata cggggcacgc agagaccgac
      301 gaggtttccc atcccgggaa tctcgatgtc ggggagcgtg tagccggcgg gaagggcgga
      361 cggcttggac aggtctgcgc agtcgatcgc gtggaccgac tcctggggga tgctgtagaa
      421 gtggaagtcg aaatgagggg cgaggaacgt ggccggcgga tgtccgtggg cctcccagtt
      481 gagtcccatg tgatcgatgc ccagcgcctt cttcgcttcc gcgggaagct ccacgactcc
      541 gagctgctcg ggcggccaca ccatctcggc gtcggccgga acggcctcga tgagcgccag
      601 gggaacggtc gcgccgagct ccacggcgcg gtcaccgtcc atggtcagcc aggtgcagac
      661 ctcggcgtcg aggacggtgg cgcaggttcg gtagacggtc ggggaagggc cggggaggac
      721 ggccgcgacg gtcgcagccg cgacggtcag gacggccgcg agtgttttcg ctttcattgg
      781 tctcgggtga tgatgggggg gtatccgggt gtattcgatg gacagtgtgt ccaatcaacg
      841 ggacgaacat agccacgccg ggagcggatg tcaagttcgc tttacaatta gtaaggcgaa
      901 aatggtcggc agcacgcccg acccgcttcc gtaccgtcca gcccgttgca ttgagccatg
      961 gcgcctcacc cacccggcgc cgtcccgccg tcaatccgcg tacggcaggt tcacgtagac
     1021 gagtccgagg tcggtgagct ctcccggcgt caatcggtgc cggccacccg cgaccgggcg
     1081 aagtcccgta cgctcgtcag cgtgacaccg aaggtctcgg cggtatcctg catgggaatc
     1141 acggagtcgt agctttccag cgcggccgcg atgccccata ccggttccgg aatgcccggg
     1201 atcggatcgc ccggagcgac gctccggatc gggatgggtc gcccggaggc ctgctcgtac
     1261 gcccggacga cctccgtcaa cgacacggcc tccggtcctc cgatcacgag cgtcgcgttc
     1321 ctcgccgccg cggtccgggt cgacgcgacg gcgaatgcgg ccacgtcctt ctccgcaacg
     1381 aaggcatgcc ggcgaagcga ctcgccgacc agggtcacgg gctctccggc gtgcatcggc
     1441 cgctcgatca gcattccgaa ccacacgtcc atgaacgggt cgggctgcag gatggtatgc
     1501 gcaatgccgc tctcgcgcag tcgctcctcg gtctctccct tcgcgcgaaa gacgggcacg
     1561 gggctgtcga ccgaggcgcc gaccgtggaa acgaagatca agtgccccac cccggacgcc
     1621 ctcgccgaat cgacgagatt gcggttgccc tggaggtcga cgttctcgac ggagtcgtca
     1681 cccgtcttcg atatgctcgc cgtcgtgatc accgcgtccg ctccctcgca cgcgcgctcg
     1741 aggcttgcgg gttgcttgag gtccccgaag accacccggg cgccggccgc ctcgagcggc
     1801 ggccagttcg acgacgggcg tgccagcgca tggacgacct cactacggtc gagcaggccg
     1861 cgtacgatct tctgaccgag cgatcctgta gcaccaacga caaggatcat ggtagcctcg
     1921 cgggaaatgg aggccgtgac ggggccgggc acctgtcgct caggccgcca ggccgcatag
     1981 ggccgcaggt tctgccgggc aagccatccg tccggacagc tcgtcatggt cccgaaaagc
     2041 cgatcgggac acggacctgg tcagttcttc aggatgaacg tgaccttgag cagcacgcgg
     2101 tactcggcga tcttcccgtt ctcgacgatc acctcctgct ccgcgatcca tgctcccttg
     2161 acgttctgga gcgtctcgct tgcgcgctcg attccgacgc gcagggcatc gtcgaagctc
     2221 gtcttcgacg cggacttgat ttccgtcact cgggctacgg acatggcaac ctctcgcttt
     2281 tgttttccgg caagggcgca ccacccgcac ccgccacggc cggcgtcact gccccgtggg
     2341 tctcagggca cggaaaggcg ccgacaacag ggtaaacgtc ggggcggagg gagtcaatcg
     2401 aggcacagag atgtggtcgc acatgggcga atca
//
LOCUS       DBNHQZ010000020         5143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1630103, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000020 DBNHQZ010000000
VERSION     DBNHQZ010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1630103"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="AC16YU_00475"
     CDS             <1..887
                     /locus_tag="AC16YU_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6063723.1"
                     /translation="AVALVAVLLTFFLVIFLVGWTLYTSTATMAGELPGYEERIGRMF
                     DNMERFVVERAAAMNIDVGEISLEDAVDVSAVRAALTSGVGSFLSFMGSMFLVLLFMM
                     FILAGSGDLAAKVRHAFPADHADRIARVIANVDSQVRRYLVTKTVVSLGTGILSFLVL
                     LIVGVDFPLIWGFLAFVLNYIPNLGSIVAVMFPVFLSALQFESFGIALLVLGLLVSCQ
                     TLMANIVEPRMMGFSLNLSPLLILVSLIFWGWLWGTWGMVLAVPLTSAIKILFENLEP
                     LRPLSVLMSQWSPRSPEG"
     gene            974..2260
                     /locus_tag="AC16YU_00480"
     CDS             974..2260
                     /locus_tag="AC16YU_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR6063724.1"
                     /translation="MKFVSAEEAVSVIESGNRVFVHTAAAAPQQLIRAMTARSDELTN
                     VEIVHLHTEGEAPYVDPKYRDAFRTNALFVGPNMRQAVADGDADYIPVFLSEIPALFR
                     RRILPVNVALIQVSTPDRHGFCSLGISVDASRAAVETANHVVAQINPNMPRTHGDGQI
                     HVRDIDFAVDVDDPIPTAHANTMGPIERAIGQHVANLIEDRSCLQMGIGAIPDAVLAA
                     LKDHVGLGIHTEMFSDGVIDLVESGVITGEYKTIHPRQIVGSFLVGSRRLYDFVDDNP
                     QVLLRECSYTNDTALIRQLPRMVAINSAIEVDITGQVVADSIGTRQYSGVGGQMDFIR
                     GASLSEGGKPIIALPSVTSRGESRIVPLPKPGAGVVTTRAHVHYLVTEHGVAYLYGKN
                     LRQRAVALIEIAHPDHREALEKEAVERFKRLRSWGY"
     gene            complement(2254..3651)
                     /gene="hutF"
                     /locus_tag="AC16YU_00485"
     CDS             complement(2254..3651)
                     /gene="hutF"
                     /locus_tag="AC16YU_00485"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HIR6063725.1"
                     /translation="MESTDAHARGMHAPYALLPDGWASDVDIRIDRRGMIEAVTTGIL
                     ESRLDRAEGPVVPGMPDAHSHAFQRLLVGRTQELTGEDDFWSWRTAMWRLAGALEPED
                     VQAVSAWVYADLVAAGYTSVAEFHYLFHDRDGTPYADPSELPLRMAAAAADARIGLTL
                     LPVLYRRSGFDGGALEGSARRFGLSLDAYVELVSRLETALSPSGIRVGIAPHSLRAVS
                     PEDITALVAWARGGPERPVHIHAAEQPAEVEGCRRATGHRPVEWLLQHGGAGSAWTVV
                     HATHTTLTERARLAASGARIALCPTTEADLGDGSFRLAEYLLSNGSFAIGSDSNAHVS
                     PASELRLLAWSARLAAGRRGALPLSAERPVGSQLWEHAACAGAGSLGLPVGRIGAGFR
                     ADLVVLDPGRPWAEGLSPDRLLDAFVLSGDPSDIARTIVAGQTVARSGRHVDAERIER
                     SARHAVRRVAGRLAQ"
     gene            complement(3658..4659)
                     /locus_tag="AC16YU_00490"
     CDS             complement(3658..4659)
                     /locus_tag="AC16YU_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6063726.1"
                     /translation="MFRPRSPTATPDGLASLHDGFSRLRTEIGKVIVGQDEIILQIFV
                     SLACRGHVLLVGVPGLAKTLLIRTLADALDLRFRRIQFTPDLMPSDITGTEIIEEDAS
                     TGRRVFKFVQGPVFANVVLADEINRTPPKTQAALLEAMQEHHVTAAGQTFVLPEPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMFNLWLDYPEFHQEVDIVRQTTSGSVAQVRHVMTG
                     QELLAAQDFVRQIPVADNVIEQAVRLVGMTRPNRDSAPEFVNQYLSYGAGPRASQYLI
                     LGAKALAALDGRLTPDLADVHRVAVPVLRHRIVTNFNAEADGVSAVDLVNRLLEAAS"
     gene            complement(4672..>5143)
                     /locus_tag="AC16YU_00495"
     CDS             complement(4672..>5143)
                     /locus_tag="AC16YU_00495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6063727.1"
                     /translation="DESTADPAPAIAPPAAVRDSILAGELTFDQAARRNSEDAASALM
                     GGRVADPQSGERKLILGALGPFWQRTLSGMAEGDISEPAEVELLDGRRAWHIVLLQER
                     IPAHRLDLASDYVMIEQYAQRDKEARVMTAWLAELRDEVYIDIRTPAPGSAPTQ"
BASE COUNT          795 a         1737 c         1786 g          825 t
ORIGIN      
        1 ccgccgtcgc cctggtcgcc gtcctgctca cgttcttcct cgtcatcttc ctggtcggct
       61 ggacgctgta cacgtcgacg gccaccatgg cgggcgagct tcccgggtac gaggaacgca
      121 tcgggcggat gttcgacaac atggagcggt tcgtcgtcga gcgagccgcc gccatgaaca
      181 tcgatgtcgg cgaaatttcg ctcgaggacg ccgtggacgt gtcggccgtg agagcagcgc
      241 tcacgtcggg cgtcggttcc ttcctgagtt tcatgggttc gatgttcctg gtcctcctgt
      301 tcatgatgtt catccttgcc gggagcggcg acctggcggc aaaggtccgg cacgcctttc
      361 ccgccgatca cgcggaccgc atcgcccggg tgatcgcgaa cgtggattcg caggtccgcc
      421 gctacctggt gacgaagacc gtggtcagcc tggggacggg catcctgagc ttcctcgtgc
      481 tcctaatcgt cggggtcgac ttcccgctca tctggggatt cctggccttc gtgctgaact
      541 acattccgaa cctgggatcg atcgtggccg tcatgtttcc cgtcttcctg tcggccctgc
      601 agttcgagtc cttcgggatt gcgctcctgg tgctgggact cctcgtctcc tgccagacgc
      661 tcatggccaa catcgtggag ccccggatga tgggattcag cctgaacctg agtcccctgc
      721 tcatcctggt gtccctgatc ttctggggct ggctctgggg gacgtggggg atggtgctcg
      781 ccgtcccgct gacgtccgcg atcaagatcc tgttcgagaa cctggagccc ctgcgcccgc
      841 tttccgtgct catgagccag tggtcgccca ggtcgccgga gggctgaatc caccctctct
      901 tcgcccatgc cgcatccggc ggcaacgcgg gggggcgtag cttgcattcc cggctaccga
      961 tcccgcccgc accatgaagt tcgtttcggc cgaagaggcc gtttccgtca tagaatccgg
     1021 caaccgcgtg ttcgtacaca ccgcggcggc cgccccccag cagctgatcc gggccatgac
     1081 ggcccggtcg gacgagctga cgaacgtcga gatcgtccac ctgcacacgg aaggggaggc
     1141 cccctacgtg gacccgaagt accgcgacgc cttccgcacg aacgccctgt tcgtcggccc
     1201 gaacatgcgg caggcggtgg cggacgggga tgcggattac atcccggtgt tcctgtcgga
     1261 gattccggcg cttttccggc ggaggatcct gccggtgaac gtcgccctga tccaggtgtc
     1321 caccccggac cggcacggat tctgctcgct gggcatctcg gtggacgcct cgcgtgcggc
     1381 ggtcgagacg gcgaaccacg tcgttgccca gatcaatccc aacatgccgc gcacgcacgg
     1441 ggacggacag atccacgtgc gggacatcga tttcgccgtc gacgtggacg accccatccc
     1501 gacggcccac gcgaacacca tggggcccat cgagcgggcg atcgggcagc acgtcgcgaa
     1561 cctgatcgag gaccgctcct gcctccagat gggaatcggg gccattccgg acgcggtcct
     1621 cgcggccctg aaggaccacg tgggcctcgg catccacacg gagatgttct cggacggggt
     1681 catcgacctg gtcgagtcag gcgtgatcac cggcgaatac aagacgattc acccgcggca
     1741 gatcgtcggc agcttcctgg tcggatcgcg acgactgtac gacttcgtcg acgacaatcc
     1801 gcaggtcctc ttgcgcgagt gctcgtacac gaacgacacc gccctcatcc gacagctccc
     1861 tcggatggtc gcgatcaaca gcgcgatcga ggtcgacatc acgggccagg tcgtcgccga
     1921 ttccatcggc acgcgccagt actcgggcgt ggggggccag atggatttca tccgcggggc
     1981 ctcgctctcc gagggcggaa agcccatcat cgcgctgccg agcgtcacgt cgcgcggcga
     2041 atcccgcatc gttccgcttc cgaagcccgg ggcgggggtc gtgaccacgc gcgcccacgt
     2101 gcattacctg gtgaccgagc acggagtggc ctacctgtac ggcaagaacc tgcgacagcg
     2161 ggccgtcgcc ctgatcgaga tcgcgcatcc cgaccaccgc gaggccctgg aaaaggaggc
     2221 cgtagagcgg ttcaaacggc tgcggagctg gggctactga gcgagccggc cggcgacccg
     2281 gcggaccgcg tggcgcgctg accgctcgat ccgctccgca tcgacgtgcc ggccggagcg
     2341 cgccaccgtc tgcccggcca cgatcgttcg ggcgatgtcg gacggatcac ccgaaaggac
     2401 gaaggcgtcg agcagccggt ccggcgagag gccctcggcc cacggacggc ccgggtccag
     2461 gacgacgaga tcggcccgga atccggcccc gatccggccc acgggcaggc caagggagcc
     2521 cgccccggcg cacgctgcgt gttcccagag ctgggagccc acgggccgct ccgctgacag
     2581 gggcagcgcg ccccggcgcc cggccgcgag ccgcgccgac caggccagga gccgcagctc
     2641 ggatgccggc gacacgtgcg cgttcgagtc ggagccgatg gcgaacgagc cgttcgagag
     2701 caggtattcc gcgagacgga acgatccgtc acccaggtcg gcctccgtgg tggggcacag
     2761 ggcgatccgc gcgccggacg cggcgaggcg ggcccgctcg gtcagggtcg tgtgggtcgc
     2821 gtggacgaca gtccacgcgg atccggcgcc tccgtgctgc aggagccact ccacggggcg
     2881 gtgtcccgtg gcgcggcggc agccttcgac ctcggccggc tgttcggccg cgtggatgtg
     2941 gacgggccgc tccggaccgc cccgcgccca ggcgaccagc gcggtgatgt cttccggcga
     3001 gaccgcccgc agggagtgtg gggcgatgcc gacgcggatg cccgacggcg agagagccgt
     3061 ctcgagccgt gagacgagct cgacgtacgc gtcgagcgaa agcccgaacc ggcgcgcgct
     3121 gccctcgagc gccccgccgt cgaatccgga tcggcggtag aggaccggca ggagcgtcag
     3181 cccgatgcgc gcgtccgcgg cggcggcggc catgcggaga gggagttccg acggatcggc
     3241 atacggcgtt ccgtcgcggt cgtggaagag gtagtggaac tcggcgacgg acgtgtaccc
     3301 cgccgccacg agatcggcat agacccacgc cgacacggcc tgcacgtcct cgggttcgag
     3361 cgcgcccgca aggcgccaca tggcggtcct ccacgaccag aaatcatcct cgcccgtgag
     3421 ttcctgggtc cggccgacca gcaggcgctg gaacgcatgg gagtgggcat ccggcattcc
     3481 gggtacgacc ggaccctccg cccggtccag ccgtgactcg aggatcccgg tcgtgaccgc
     3541 ctcgatcatg ccccgcctgt cgatccggat gtccacgtcg gaagcccagc cgtcgggaag
     3601 cagcgcgtac ggcgcgtgca ttccgcgggc atgggcatcc gtggattcca tcgctcgtca
     3661 ggaggcggcc tcgagcaggc gattcaccag gtccacggcc gatacgccgt cggcttcggc
     3721 attgaaattc gtcacgatgc gatgccgcag caccggaacg gcgacccggt gcacgtccgc
     3781 gaggtccggc gtcagcctgc cgtcgagggc ggccagcgcc ttggcgccga gaatcaggta
     3841 ctgcgaggcg cgcggtccgg cgccgtacga gaggtactgg ttcacgaact ccggcgcgga
     3901 gtcacggttc ggacgggtca tgcccacgag ccgcacggcc tgttcgatga cgttgtccgc
     3961 gacgggaatc tgccgcacga aatcctgcgc cgccaggagc tcctgccccg tcatgacatg
     4021 acgcacctgg gcgacgctgc ccgaggtcgt ctgccggacg atgtcgacct cctggtggaa
     4081 ttccgggtaa tccagccaga ggttgaacat gaaccggtcg agctgcgcct cgggaagggg
     4141 ataggtgccc tcctgctcga tgggattctg cgtggcgagc acgaagaagg gctccggaag
     4201 cacgaaggtc tgtccggcgg ccgtcacgtg gtgctcctgc atggcctcca ggagcgccgc
     4261 ctgggtcttg gggggagtcc ggttgatctc gtccgccagg accacgttcg cgaacaccgg
     4321 gccctgcacg aacttgaaga cccggcgccc ggtcgaggcg tcctcctcga tgatctcggt
     4381 cccggtgatg tcggagggca tcaggtcggg cgtgaactgg atccgtctga acctgaggtc
     4441 cagggcgtcg gcgagcgtgc ggatcaggag agtcttggcg aggccgggca ctccgacgag
     4501 cagcacgtgt ccgcggcacg ccaggctgac gaagatctgg aggatgatct cgtcctgccc
     4561 gacgatcacc ttgccgatct cggtccgcag ccggctgaac ccgtcgtgga gggaagcgag
     4621 tccgtcagga gttgcggtgg gagacctggg ccggaagatt gcggttgcgg atcactgggt
     4681 cggcgccgag ccgggggcgg gcgtgcgtat gtcgatgtag acctcgtcgc gaagctcggc
     4741 gagccacgcg gtcatgacgc gcgcttcctt gtcccgctgg gcatactgtt cgatcatcac
     4801 gtaatcggac gccaggtcga gtcggtgggc cggaatccgc tcctggagca ggacgatgtg
     4861 ccaggccctg cgcccgtcga gcagttcgac ctcggcaggc tcgctgatgt ccccctctgc
     4921 catgcccgag agcgtgcgct gccagaaggg acccagggcg ccgaggatca gcttgcgctc
     4981 gccgctctgc ggatccgcca cgcggccgcc catgagagcg ctcgcggcgt cctcggagtt
     5041 ccggcgggcc gcctggtcga aggtcagttc tccggccagg atcgaatcgc ggacggcggc
     5101 cgggggggcg atggcgggtg ccgggtccgc cgtggactcg tcg
//
LOCUS       DBNHQZ010000021         5326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_952513, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000021 DBNHQZ010000000
VERSION     DBNHQZ010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_952513"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="AC16YU_00500"
     CDS             <1..1613
                     /locus_tag="AC16YU_00500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6063728.1"
                     /translation="GRSLDGRLLVIASRDSTSEPRFQTRPGVQAVQVFGVDVDGADPG
                     SRLEIDGSVFGYPAERTSDLEPGRYWVQAVLHLYETFNLETGHTVKLPLPRGAGQNWR
                     LEPGNLYSTPSWVEIGSGGEIELTLDQVIPDIEPPRDTEYVRHVRIRSERLSEFWGRD
                     MYLGAHVLLPYGFDQHPEARYPLMVFHGHFPADFGGFRTSPPDPTVPCEYSERFRLDC
                     YNRIVDQEAYDFYRTWTSRDFPRFLVIEIQHDNPYYDDSYAVNSANLGPYGDAIQYEL
                     IPAIEQQFRGIGQGWARFTYGGSTGGWEALAVQIFYPDEYNGAFGACPDPIDFRQYTQ
                     VNIYEDANAYWQKGPFRTMERPAHRNWLGHVDIMQRDYNHYELALGTRGRSGDQYDIW
                     QAVYSPMGEDGYPKPIWDKLTGEIDRDVAEYWRENYDLRHILERDWAELGPKLRGKLH
                     IYCGDMDNYYLNNAVYLMEEFLESTTDPYYGGIVDYGDRAEHCWNGDHENPNAISRLR
                     YNTMYVPRILQRIRDDAPPGADLSSWRY"
     gene            complement(1607..4846)
                     /locus_tag="AC16YU_00505"
     CDS             complement(1607..4846)
                     /locus_tag="AC16YU_00505"
                     /inference="COORDINATES: protein motif:HMM:NF053442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6063729.1"
                     /translation="MRVAALVSAVALLSGALPSSAQVQGYYRFPTVHEVTVVFTSEGD
                     LWVASVAGGTAHRLTTHAGQEAFPVLSPDGRRVAFAASYEGAPEVYVMPVEGGTPTRL
                     TWDGGSSLPVAWSPDGRVVYRTSRYSTLPSVQLVAVDPATLERERIPLYEAAEVAYGP
                     DGTLVFTRQPRQGSNSRWYKGGSAQNLWKWKPGDAAATPLTADWPGTSKAPYWHDGRI
                     VFLSDRGGMGMNVWSMAPDGSDVRPHTRHMDWDIQEQSVQGSTVVYRLGAGLHRLDLA
                     TGEESPIALDLVSDREQRRVTWEDAPADYVTSAELSPDGSRLVLAARGEVFVAPVEGG
                     RVANASRDSGVRHRYARFSADGRSLLTLSDASGEVEWWSLPVDGVGEARQITRGPAML
                     RLSGIPSPDGRWIAEQDYDRRIWITDLATGEGRVVSESPGQWASQGIGYAWSPDSRHL
                     AYHVVGTTRLSELHLYDVSDGSSKRLSSGRFEDWDPAWSPDGNWLYFLSNRDFEVTGS
                     VWGARATQPHFDRQTRIHAIALSDGARWAFREPDEITPPDTGKAMPPARTGPPSPGPL
                     RVVPVDRGNYRSLATNGKRLFWTANEPGETGQVLRAIELEHDAKPVEVAKGIRGYLLS
                     QDGKKLMIRKGEDVFVVDATAGAEAKLEDGKVDLSGWQLEVDKDAEWRQIFVDAWRLH
                     RDYFYDPGMHGVDWEAVRAKYEPLVGRIGAREELADLQAQMISELSLLHSNASGGDTR
                     SGSDDVEPASLGAVFARDGATGGFRVEHVYRTDPDLPGTRAPLAHPDVAVAEGAVILS
                     VNGRHASGAPDIGALLRGEAGRQVRLRVQDADGTGRDVVARPVTMDEEFDLRYSEWEY
                     TRRLAVDEMGGGRIGYVHLRAMGGGDMGQWTRDFYPVFDRQGLVIDMRRNGGGNIDSW
                     VLTQLLRRAWMYFKGRTGDPYWNMQHAFRGHMVVLVDWRSGSDGEAFPDGFRRLGLGK
                     IIGTRTWGGEVWLSSSNRQVDGGVARASETGVYGPEGEWLIEGWGVVPDQVVDNDPYQ
                     AFNGRDSQLEAAIEHLERLIAEDPRLVPDPPPYPLVRPGRGYPTPYERQ"
     gene            5016..>5326
                     /locus_tag="AC16YU_00510"
     CDS             5016..>5326
                     /locus_tag="AC16YU_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063730.1"
                     /translation="MINAPGWRDVLLAEKDGGFRAHVQDLTARGMRALGYVGPAAIAL
                     YIALSLAAGREYTLGAGEPGAFAIWDKLVILLLSASLVPLSRIDAAQRAGRWIGMAAG
                     VV"
BASE COUNT          816 a         1974 c         1742 g          794 t
ORIGIN      
        1 ccggcaggtc tctcgacggc cgcctcctcg tcatcgccag ccgcgattcc acgtccgagc
       61 cgcgcttcca gacccggccc ggcgtccagg cggtccaggt cttcggcgtc gacgtggacg
      121 gggccgatcc aggaagccga ctggaaatcg acgggagcgt gttcggctat ccggcagagc
      181 gaacctcgga cctcgagccc ggccggtact gggtgcaggc cgtgctccac ctctacgaga
      241 ccttcaacct ggagacgggc cacaccgtca agctgccgct cccccgcggg gcgggccaga
      301 actggcgact cgagcccgga aacctgtact cgacgccctc ctgggtcgag atcggcagcg
      361 ggggcgagat cgagctgacc ctcgaccagg tgattccgga catcgaaccg ccccgcgaca
      421 cggaatacgt gcggcacgtc cggatccgga gcgagcggct ctccgagttc tggggacgcg
      481 acatgtacct cggggcccac gtcctcctgc cctacggctt cgaccagcac cccgaggccc
      541 gctacccgct catggtgttc cacgggcact tcccggccga tttcgggggc ttccgcacct
      601 cgccgcccga cccgacggtc ccctgcgaat acagcgagcg cttccggctg gattgctaca
      661 accggatcgt tgaccaggag gcctacgact tctaccggac ctggacgtcg cgcgacttcc
      721 cgcgcttcct ggtgatcgag atccagcacg acaacccgta ctacgacgac tcctacgcgg
      781 tcaactccgc gaacctcggg ccgtacggcg acgccatcca gtacgagctc atccccgcga
      841 tcgagcagca gttccggggc atcggccagg gctgggcgcg gttcacgtac ggcggctcga
      901 cgggcggctg ggaggccctc gcggtgcaga tcttctatcc cgacgagtac aacggcgcct
      961 tcggggcctg ccccgacccg atcgacttcc gccagtacac gcaggtcaac atctacgagg
     1021 acgcgaacgc ctactggcag aaggggccgt tccgcaccat ggagcgtccc gcccaccgca
     1081 actggctcgg gcacgtggac atcatgcagc gggactacaa ccactacgag ctcgcgctcg
     1141 ggacccgcgg ccggtcgggc gaccagtacg acatctggca ggcggtctac tcgcccatgg
     1201 gcgaggacgg ctaccccaaa cccatctggg acaagctcac cggcgagatc gaccgcgacg
     1261 tcgccgaata ctggcgcgag aactacgacc tcaggcacat cctcgagcgc gactgggccg
     1321 agctcgggcc gaagctccgc ggcaagctcc acatctactg cggggacatg gacaactact
     1381 acctgaacaa cgccgtgtac ctgatggagg agttcctgga gtcgaccacc gacccgtact
     1441 acggcgggat cgtggactac ggggaccgcg ccgagcactg ctggaacggc gaccacgaga
     1501 acccgaacgc gatctcgcgc ctccgctaca acacgatgta cgtgccgcgc atccttcagc
     1561 ggatccggga tgacgcgccc ccgggggccg acctctccag ctggcgctac tgacgttcgt
     1621 acggcgtggg atagccccgc cccggacgca ccagcgggta gggcggcggg tcgggcacca
     1681 ggcgcggatc ctcggcgatc agccgctcca ggtgctcgat cgcggcctcg agctgcgagt
     1741 cccggccgtt gaaggcctgg tacgggtcgt tgtcgacgac ctggtccggg accacacccc
     1801 atccctcgat gagccactcg ccctccggcc cgtagacccc ggtctcggac gcccgtgcga
     1861 cgccgccgtc cacctggcgg ttcgagctcg acagccacac ctcgccgccc cacgtcctcg
     1921 tcccgatgat cttgcccagg cccagcctgc ggaagccgtc ggggaacgct tcgccgtccg
     1981 acccggaccg ccagtccacg agcaccacca tgtggccccg aaaggcatgc tgcatgttcc
     2041 agtacgggtc gcccgtgcgg cccttgaagt acatccaggc ccgccgcagg agctgcgtca
     2101 acacccagct gtcgatgttg ccgcccccgt tgcgccgcat gtcgatgacg aggccctgcc
     2161 ggtcgaacac cgggtagaag tcccgcgtcc actggcccat gtcgccgccg cccatcgccc
     2221 gcaggtgcac gtacccgatg cgcccgccgc ccatctcgtc cacggcgagg cggcgcgtgt
     2281 actcccactc ggaatagcgc aggtcgaact cctcgtccat cgtgacgggc ctggccacga
     2341 catcgcgccc ggtcccgtcc gcgtcctgca cacgcaggcg cacctgccgc ccggcctcgc
     2401 cgcggagcag ggcgccgatg tccggcgccc cggaagcgtg cctgccgttg accgacagga
     2461 tgacggcccc ctcggcgacg gccacgtccg gatgcgccag cggcgcccgg gtccccggaa
     2521 ggtcgggatc ggtgcggtac acgtgctcga cacggaatcc gcccgtagcc ccgtccctcg
     2581 cgaagaccgc gccgagcgag gcgggctcga catcgtcact gcccgagcgc gtgtcccctc
     2641 ccgaggcatt cgagtgcagg agcgagagct cgctgatcat ctgggcctga aggtcagcga
     2701 gctcctcgcg cgcgccgatg cgcccgacga gcggctcgta cttcgcccgg acggcttccc
     2761 agtccacccc gtgcatcccc gggtcgtaga agtagtccct gtgcaggcgc cacgcgtcca
     2821 cgaagatctg gcgccattcg gcgtccttgt ccacttcgag ctgccagccg gacaggtcca
     2881 ccttcccgtc ttcgagcttc gcttccgcgc cggccgtcgc gtccacgacg aagacgtcct
     2941 cgcccttgcg gatcatcagc ttctttccgt cctgcgagag caggtagccc cggattccct
     3001 tcgcgacctc gacgggcttg gcgtcgtgct cgagctcgat cgcccgcagg acctgccccg
     3061 tctcgcccgg ctcgttcgcc gtccagaaca gccgcttgcc gttcgtggcc agggaacggt
     3121 agttcccccg atcgacgggc acgacgcgca gagggccggg cgacggcggg cccgtccgcg
     3181 cgggcggcat ggcctttccg gtgtccggag gcgtgatttc gtccggctcg cggaaggccc
     3241 agcgggcccc gtcggagagc gcgatggcat ggatccgggt ctgccggtcg aagtgcggtt
     3301 gcgtggccct cgcgccccac acgcttccgg tcacctcgaa atcccggttc gagaggaaat
     3361 acagccagtt cccgtccggc gaccaggcgg gatcccagtc ctcgaagcgg cccgacgaca
     3421 ggcgcttcga agagccgtcc gacacgtcgt acaggtggag ctcggacagg cgggtcgtgc
     3481 ccacgacatg gtaggccagg tgccggctgt ccggcgacca cgcgtatccg atgccctggg
     3541 aggcccactg cccgggtgac tccgacacga cgcgaccctc ccccgtcgcg aggtccgtga
     3601 tccagatccg gcgatcgtag tcctgctcgg cgatccaccg tccgtcgggc gacggaatgc
     3661 cggacagtcg cagcatcgcc ggaccccggg tgatctgccg ggcctctccg acgccgtcca
     3721 cgggcaggct ccaccactcc acctcgcccg aggcatccga cagcgtgagc aggctgcggc
     3781 cgtccgcgga gaaccgggcg tagcgatgcc ggacgccgga gtcccgcgac gcgttggcca
     3841 cgcggccacc ctcgaccggc gcgacgaaga cctcgccgcg ggcggccagg accagccggc
     3901 ttccgtcggg cgagagctcg gcggacgtca cgtaatcggc cggtgcgtcc tcccaggtca
     3961 cccgccgctg ctcccggtcg gagaccaggt ccagcgcaat gggagactct tcgcctgtcg
     4021 ccaggtccag ccggtgcagc ccggccccga gccggtacac gaccgtcgag ccctgcacgg
     4081 actgctcctg gatgtcccag tccatgtgcc gggtgtgggg acggacgtcc gagccgtcgg
     4141 gcgccatcga ccacacgttc atgcccatgc ccccccggtc cgacaggaac acgatccgcc
     4201 cgtcgtgcca gtacggagcc ttggacgttc ccggccagtc cgcggtgagg ggcgtcgcgg
     4261 ccgcgtcgcc cggcttccac ttccagaggt tctgcgccga gccccccttg taccagcgcg
     4321 agttgctccc ctgccggggc tggcgcgtga agaccagggt cccgtcgggc ccgtaggcca
     4381 cctcggcggc ctcgtagagc ggaatccgct cccgctcgag cgtcgcggga tccacggcga
     4441 cgagctgaac gctcggcagc gtgctgtagc gcgaggtccg gtacacgacg cggccgtccg
     4501 gcgaccaggc caccggcagc gacgagccgc cgtcccaggt cagccgggtc ggagtcccgc
     4561 cctcgaccgg catcacgtac acctccggcg ctccctcgta gctcgcggcg aacgccacgc
     4621 gccggccgtc gggcgagagt accgggaagg cttcctgtcc tgcgtgcgtc gtcagcctgt
     4681 gggccgttcc acccgccacg gaggccaccc agagatcccc ctcggacgtg aagaccaccg
     4741 tgacctcgtg gacggtcggg aatcggtagt aaccctggac ctgggcggac gacggaaggg
     4801 cgcctgaaag gagggcaacg gcggagacga gggcggcgac gcgcatggct cgtagggacc
     4861 tggtgatggg ttccgcaatg ctacgccgtt cttcggcgac gtgcagggtt tgttgcggca
     4921 gcccgcccgc gtccgcgatc cccgtagggt ttactccggc ttgactcctc ctttcccctt
     4981 cggtttattc gggataatct tccgtcgcgc cggccatgat caatgctccc ggatggcgtg
     5041 acgtgctgct tgcagagaag gatggcggat tcagggcgca cgtccaggat ctcaccgccc
     5101 gcggcatgcg ggcactcgga tacgtagggc cggccgccat cgcgctgtac atcgcgctca
     5161 gcctggcggc cggacgagag tacaccctcg gggccggcga gccgggcgcg ttcgcgatct
     5221 gggacaagct cgtcatcctc ctgctgtccg cctccctggt cccgctctcg cggatcgacg
     5281 cggcgcagag ggccgggcgg tggatcggca tggcggcggg cgtcgt
//
LOCUS       DBNHQZ010000022         2011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1494721, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000022 DBNHQZ010000000
VERSION     DBNHQZ010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1494721"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="AC16YU_00515"
     CDS             complement(<1..1273)
                     /locus_tag="AC16YU_00515"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR6063731.1"
                     /translation="MAHPNPWETLLDMPGVTASLQRSVARLVGALHEATPEPVARVLN
                     RVHEALSRPDGLDGAARFLEASSDEEIEAMIRFQTTLFHLLNQAEKQEIIRVNRERAI
                     RAGESDGRPESIAATARALAADGLGADAFGRFLERLDVGPTLTAHPTEARRRSVLSKQ
                     KAIGEAIAALEGRALTPAEAAEAEYRMRRAVHLLIATDEVRSRDVTVQDEVEYGLHFL
                     AGTIWEVLPRIAHDLRDAARRHFGFTPGTLAPLRFRSWIGGDRDGNPNVTPQVTRETA
                     ARLRAAAIDLNLAALADLRRILSISEDRVVVSEALARSLRRDLEEVDPGEMARRHYAA
                     EPFRLKIACMEARLRAEGAQGAASTYDARRYRADLDLIADALGAAGLDVSVRTGPLAA
                     LRDRVAATGLHLAALDIRQHSSRHEEAVAEIL"
     gene            1427..>2011
                     /locus_tag="AC16YU_00520"
     CDS             1427..>2011
                     /locus_tag="AC16YU_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6063732.1"
                     /translation="MTTDPTSRDFLRNLGMDDHNAGGFAGEWVGSGPELEATTPIDGS
                     RIATVRQVTEDEFDAIVARAHEAFLVWRTVPAPRRGEIVRQLGLRLREYREDLGRLVT
                     LEMGKIAAEGLGEVQEMIDICDLAVGQSRQLYGLTMSSERVDHRMMEQWHPLGVVGVI
                     SSFNFPVAVWSWNTALAIVCGDAVLWKPSSETPLT"
BASE COUNT          267 a          716 c          726 g          302 t
ORIGIN      
        1 gcaggatctc ggccacggcc tcttcgtgcc gggagctgtg ctgccggatg tcgagcgccg
       61 ccaggtgcag gcccgtggcc gccacgcggt cccggagcgc agcgagcggg cccgtccgga
      121 cgctgacgtc gagtccggcc gcgccaagcg cgtcggctat caggtccagg tccgcccggt
      181 accttcgggc gtcgtaggtc gaggcggcgc cctgggcgcc ctccgcgcgg agtcgcgcct
      241 ccatgcaggc gatcttgagg cggaagggtt ccgccgcgta gtgccggcga gccatctcgc
      301 ccgggtccac ctcctcgaga tcccgccgca ggctgcgggc gagcgcctcg gaaaccacca
      361 cgcggtcctc cgatatgctc aggatgcgcc gcaggtcggc cagcgccgcg aggttcaggt
      421 cgatggccgc cgcgcgaagc cgggcggccg tctcccgcgt gacctggggc gtcacgttcg
      481 ggttgccgtc gcggtcgccc ccgatccacg agcggaagcg caggggagcg agggtgccgg
      541 gcgtgaaccc gaagtgcctg cgcgccgcgt cccgcaggtc gtgcgcgatc cggggaagca
      601 cttcccagat cgttcccgcc aggaaatgca gcccgtactc gacctcgtcc tggacggtca
      661 cgtcgcgcga gcggacctcg tcggtggcga tcagcaggtg tacggcccgg cgcatccggt
      721 actccgcctc ggccgcttcc gcgggcgtga gcgcccggcc ctcgagcgcg gcgatggcct
      781 cgccgatggc tttctgcttg ctgaggacgc tgcggcgccg cgcctcggtc gggtgcgcgg
      841 tgagcgtcgg cccgacgtcc aggcgttcca ggaaacggcc gaacgcgtcg gcgcccagtc
      901 cgtcggccgc aagggcgcgc gcggtcgcgg cgatcgattc aggccgaccg tccgactcgc
      961 ccgcccggat cgcccgctcc cggttcaccc ggatgatctc ctgcttctcg gcctggttga
     1021 gcaggtggaa gagcgtcgtc tggaaccgga tcatcgcctc gatctcctcg tcgctcgacg
     1081 cctcgaggaa gcgggcggcg ccgtcgagcc cgtccgggcg ggacagggcc tcgtggacgc
     1141 ggttcaggac gcgggcgacg ggctccggcg tcgcctcgtg cagcgccccg acgagccgcg
     1201 cgaccgagcg ctggagcgac gccgtgactc cgggcatgtc gaggagcgtt tcccagggat
     1261 tcggatgggc catggccggg gcgttgcgtt gccccgtgta cgtcgcgggc ggcaggccgt
     1321 tcgccccgat acgtggaata cgggtccgaa ttcgaaccat cgtcacgctc cgctcgcccc
     1381 tcctgcgtaa cattcggctt cccgaaccaa tgtctctccg cacgcgatga cgaccgatcc
     1441 gaccagccgc gatttcctga ggaacctggg catggacgac cacaacgccg gcggcttcgc
     1501 gggcgagtgg gtgggctcgg gccccgagct cgaggcgacc acgcccatcg acgggtcgcg
     1561 catcgcgacc gtccgccagg tgaccgagga cgagttcgac gccatcgtgg cgcgcgccca
     1621 cgaggccttc ctcgtctggc gtaccgtacc ggcacctcgc cggggcgaga tcgtccggca
     1681 gctgggcctg cgcctgcgcg aatacaggga ggacctcggg cgcctggtca cgctcgagat
     1741 gggcaagatc gcggccgagg gcctgggcga ggtgcaggag atgatcgaca tctgcgacct
     1801 cgccgtcggc cagtcgcgcc agctctacgg gctcacgatg tcctcggagc gggtcgatca
     1861 ccggatgatg gagcagtggc acccgctcgg cgtggtcggc gtcatcagct cgttcaactt
     1921 tcccgtggcg gtctggagct ggaacacggc gctggcgatc gtctgcggcg acgcggtgct
     1981 ctggaagcca tcctccgaaa ccccgctcac g
//
LOCUS       DBNHQZ010000023         2630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1766941, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000023 DBNHQZ010000000
VERSION     DBNHQZ010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1766941"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="AC16YU_00525"
     CDS             complement(<1..1218)
                     /locus_tag="AC16YU_00525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6063733.1"
                     /translation="MHRTLRLLPVLLVLLLGPAATAQTVDLAAIDAWIAQAQRDWPVP
                     GLSVAIVKDGEIVLEKGYGVRHATDGGAADEHTLYAIASNSKAFTAAALAQQVDAGVL
                     SWDDHVIDHLPYFRLYDDYVTQEMRVRDLLSHRSGLGTFSGDLLWYGTPYTAEEVVRR
                     VRHLPPAYPFRAGYGYTNVMFVAAGEVLRSVTGQGWHDYVEEHFFAPLGMDRSVTSVR
                     DLASRENVATPHKNERGQVLPIEWYVWDAMGAAGGIISSVHDMSQWMIAQLGGGEKDG
                     TRLFSEDAQRTMWTVHNPFGLPRGYLDGPISSHFRGYGLGWSLRDQEGRLVVSHGGGY
                     DGMYSNLTLVPEEDLGVVVLTNSMTGVTGVITDRIIAAYLGGEVPDGSAEALERWKAS
                     RDEFHGRQDRAEAE"
     gene            1424..>2630
                     /locus_tag="AC16YU_00530"
     CDS             1424..>2630
                     /locus_tag="AC16YU_00530"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6063734.1"
                     /translation="MTRSLLSGVHGALVLLSALALTRPALGQGVTTAAISGLVTDTSG
                     QPLPGANVVAVHQPSGTTYGTAVRDGGLYNLPNLRIGGPYTITVSFIGFETKREENVF
                     LNLAQNLQLSFVLAAGVVSGEEIIVTADMDEVMSPDRTGAATTISPDQIALMPTIKRS
                     TRDLTRLDPRSDGNLSFGGRNWLYNNISLDGSYFNNPYGLDAPEPGGQSNAQPVPYDA
                     IEQVQVSVAPFDVREGGFTGAGINQVTKSGTNEYTGSIYAFTRNESFVGNKVSGTTVF
                     KNPELSFTQAGLSLGGPIMRNRLFFFVNGEIERREDPGSNFRASDGTPGPGESRVSAA
                     VMDQIRQRLIDVYDYDPGVYQGYSHDTENEKLLAKLDFNVNTSNTLSLRYNYMKAFRE
                     QGPHPFVLSF"
BASE COUNT          449 a          882 c          868 g          431 t
ORIGIN      
        1 ctccgcctcg gcgcggtcct gccgcccgtg gaactcgtcg cgcgacgcct tccagcgctc
       61 gagcgcctcg gcgctgccgt ctggcacctc gcctcccagg taagccgcga ttatgcggtc
      121 cgtaatgact cccgtgacgc ccgtcatcga gttcgtgagc acgacgacgc cgaggtcctc
      181 ctcgggaacg agcgtaaggt tcgagtacat gccgtcgtag ccgccgccgt ggctcacgac
      241 cagcctgccc tcctggtcgc ggaggctcca gcccagtccg tacccgcgga aatgcgagga
      301 gatgggtccg tccaggtagc cgcgcggcag gccgaacggg ttgtgcaccg tccacatggt
      361 gcgctgggcg tcctcggaga agagccgcgt tccgtccttc tctccgccgc caagctgggc
      421 gatcatccac tgggacatgt cgtgcacgct cgagatgatg ccgcccgccg cccccatggc
      481 gtcccagacg taccactcga tggggaggac ctggccgcgc tcgttcttgt gcggcgtcgc
      541 cacgttttcg cgcgaggcga gatcgcggac ggacgtcacc gagcggtcca tgccgagcgg
      601 ggcgaagaag tgctcctcga cgtagtcgtg ccagccctgt cccgtcaccg agcgcagaac
      661 ctcgccggcc gcgacgaaca tgacgttcgt gtacccgtaa ccggcccgaa acgggtaggc
      721 gggcggcagg tgtcgcacgc ggcgaaccac ctcctcggcc gtgtacgggg tcccgtacca
      781 caggaggtcg cccgagaacg tcccgaggcc gctccggtgc gagagcaggt cacgaacgcg
      841 catctcctgc gtcacgtagt cgtcgtagag ccggaagtac ggcaggtgat cgatgacgtg
      901 atcgtcccac gagaggacgc ccgcgtccac ctgctgcgcg agcgccgcgg ccgtgaaggc
      961 cttggagttg gacgcgatcg cgtagagcgt gtgctcgtcc gcggccccgc cgtccgtggc
     1021 gtgtcggacg ccgtagcctt tttcgaggac gatctcgccg tccttgacga tggccaccga
     1081 aaggccgggc accggccagt cccgctgcgc ctgggcgatc caggcgtcga tggctgcgag
     1141 gtcgacagtc tgggcggtgg ccgccgggcc gagaaggagg acgaggagaa cggggaggag
     1201 gcggagcgtc cggtgcatgg cgcggtcgaa ggtagtgcgt gaacggatcg acgggcgccc
     1261 ggtaactacc tcgcgcgggc gtattccgca agccgacggg cggcgtgacc cccgggtggg
     1321 ccgtgccgtg gatgaatgaa tgccagggaa gctgctcgct gttttcacct cgcgcgccgc
     1381 cggctacatt cccccgtccc caaccgtagc atggaggtct ctcatgacgc gtagcttact
     1441 gtccggagtg catggcgcac tcgtgctcct gtctgctctc gcactcaccc ggcccgccct
     1501 gggtcagggt gtaaccaccg cggccatcag cggtctcgtg accgatacgt cgggtcagcc
     1561 tctgcccggc gcgaacgtgg tggcggttca ccagccgagc ggcaccacct acggcacggc
     1621 cgtgcgtgac ggcggcctgt acaacctgcc gaacctccgg atcggcggcc cctacaccat
     1681 cacggtctcg ttcatcgggt tcgagacgaa gcgggaagag aacgtctttc tgaacctggc
     1741 ccagaacctg cagctgtcgt tcgttctggc agccggcgtc gtatccggag aagagatcat
     1801 cgttacggcc gatatggacg aggtcatgag ccccgaccgc accggcgccg caacgaccat
     1861 cagtccggat cagatcgcgc tcatgccgac catcaagcgt tcgacgcgcg acctcacgag
     1921 actcgatccg cgctcggacg gcaacctgag cttcggcggc cggaactggc tctacaacaa
     1981 catctcgctc gacgggtcct atttcaacaa cccgtacgga ctggatgccc cggagcccgg
     2041 cgggcagtcc aacgcgcagc ccgtcccgta cgacgccatc gagcaggtgc aggtctccgt
     2101 cgctccgttc gacgtgcggg agggcggatt caccggggcg ggcatcaacc aggtcacgaa
     2161 gagcggcacg aacgagtata ccggatcgat ctacgccttt acccgaaacg agagcttcgt
     2221 cggcaacaag gtgtcaggga cgacggtgtt caagaatccg gagctgtcgt tcacccaggc
     2281 cggcctgtcg ctgggtgggc ccatcatgcg gaacaggctc ttcttcttcg tgaacgggga
     2341 gatcgagcgg cgcgaggatc cgggctcgaa tttccgcgcg agcgacggca ctccggggcc
     2401 cggcgagtcg cgcgtttcgg ccgccgtgat ggaccagatc cggcagcgcc tcatcgatgt
     2461 ctacgactac gatccgggcg tgtaccaggg ctactcccat gacacggaga acgaaaagct
     2521 gctggcgaag ctggatttca acgtgaacac gagcaacacg ctgagccttc gctacaatta
     2581 catgaaggca ttccgcgaac agggcccgca cccgttcgtg ctgtcgttcg
//
LOCUS       DBNHQZ010000024         5136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1767418, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000024 DBNHQZ010000000
VERSION     DBNHQZ010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1767418"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="AC16YU_00535"
     CDS             complement(<1..188)
                     /locus_tag="AC16YU_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063735.1"
                     /translation="MPGSGAEPFRLEEESRGTLAASILPDTKKPSPEGEGERGTAGGA
                     GLLHLPRSSRIEQELAPGR"
     gene            274..1464
                     /locus_tag="AC16YU_00540"
     CDS             274..1464
                     /locus_tag="AC16YU_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6063736.1"
                     /translation="MHPQTRLALAGQKTDRSFGSVALPIYQSAIFRFGEDGEKPEFDY
                     SRSGNPTRLALERLLAELEGGNDAVAVGSGMAAIATLLSLYDADAHVICSHDCYGGTE
                     RLFTWLAGQGKLSVSFVDLTDPANLAAAVRPSTRAVWVETPSNPLLRIVDLRAIGLFC
                     EANGLDFIADNTFLSPLLQRPIEFGADYVVHSTTKYLNGHSDVVGGAIVARTADQAEA
                     IRFASNTLGTTAVPFDSWLVLRGLKTLPVRLRQHEENAHRVARFLERHDGVRRVFYPG
                     LETHEGHELAARQQSGFGGMVSFEVDSGRVDVRILLRSTTVFTLAESLGGVESLIEQP
                     STMSHASMRPEARLQAGITDQVVRLSVGIEHGDDLVDDLARAFDVAARIPVRRRQNAA
                     AVRI"
     gene            1562..2323
                     /locus_tag="AC16YU_00545"
     CDS             1562..2323
                     /locus_tag="AC16YU_00545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6063737.1"
                     /translation="MRIDLTDHVILVTGASRGIGRETARQLAAAGATVAVHYGRGAEE
                     AAALAAEIGNGSRAFGADLADAGETVKLFRDVVAAYGHVDTLVNNAGIALNVPVAAEL
                     DHWVREWDATLDVNARASALLAREAVAHFLDRGRGGRIIFIASRAAFRGDTPDYLAYA
                     ASKGAMVSMARTLARGYGKQGIKAFTVAPGFVRTDMAQNAIDEYGEGFVVNDLALDRL
                     TEPADVAPFVVLLSSGLSDHATGATIDINAGSYVH"
     gene            2301..3290
                     /locus_tag="AC16YU_00550"
     CDS             2301..3290
                     /locus_tag="AC16YU_00550"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR6063738.1"
                     /translation="MPAATFTDPPVKVLKFGGTSVGDLDGLKRVLSIIRGELVAYRAI
                     VVVSATSGTTDTLVDLARVEPAGRDALVDGIRDRHEALARDLLGDSAHATYLRLLRER
                     LDELRRVLAEPDAPERTAAVLATGERLSAPLVALGLERLGLEATAVDAVDLISTDSDF
                     GAATVDLAATRRTVEAWFAILPPNVVPVVTGFIGRSSCGRTTVLGRGGSDYTASLLAE
                     AVSATKLDRWTDVDGLHTADPRRFDGTGRFLYLLLEDASTWNETSRLGMHPQAFEPVR
                     RACIPVHVRSTRHPQGDGTLILPRRLAGHVRTHSRNAMKRAAHRERGSEAQSE"
     gene            3290..4348
                     /gene="asd"
                     /locus_tag="AC16YU_00555"
     CDS             3290..4348
                     /gene="asd"
                     /locus_tag="AC16YU_00555"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6063739.1"
                     /translation="MNGKLRAGILGATGAVGQKFVELLAEHPWFEVTALAASERSAGR
                     RYRDVVRWIGTGRVPDAVGDLEVRPSEAPLECDFVFSGLDASVAGDVETAFAAAGYPV
                     ISNARNHRMDADVPLLVPEVNPDHVALVDRQAFGGGGFIVTNPNCSTVGLTMALAPLH
                     RAFGVRDVAVTTFQALSGAGYPGVASLDAVGNVVPFIGGEEAKLVAEPRRILGTLAPG
                     GVEDADIAISPQCNRVPVIDGHLECVSVRFERKPDSIAEVAECLRGFASPDVVVGLPS
                     APALPVQVLDAADAPQPRRDVMAGRGMTVSVGRVRACEVLDAKFVLLVHNTIRGAAGG
                     AILNAELLVRTGYLKPRS"
     gene            complement(4317..>5136)
                     /gene="galK"
                     /locus_tag="AC16YU_00560"
     CDS             complement(4317..>5136)
                     /gene="galK"
                     /locus_tag="AC16YU_00560"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR6063740.1"
                     /translation="WEGGVAGDVPGGAGRSSSAALEIATLRAAPALPGEPWRADRMAR
                     LGQEAENRWVGMNCGIMDQMLSAGGREGTALLIDCRSLETEPVTLPAGTVAVVMDTAT
                     RRGLVDSEYNERRASCEEAARRLGVRALRDVSLRDFLAREGGLGDTIRRRARHVVTEN
                     ERTLEAAGTSDPERLGELMDESHESLRDDFEVSTSRLDLMAELARETDGCFGARMTGA
                     GFGGCAVALVAEDAAEAFTSSVSRRYATRSGLEPMLFATAAADGATIEASDSLS"
BASE COUNT          721 a         1890 c         1774 g          751 t
ORIGIN      
        1 cgccctggtg ccaattcctg ctcgatgcga gaagatcggg gaagatggag gaggccggcg
       61 ccgcccgcgg tgccgcgctc accctcccct tccggcgagg gttttttcgt atcgggaagg
      121 atggaagcgg cgagggtgcc tcgggactcc tcttcgagcc gaaacggctc ggcgccgctt
      181 ccgggcacgg ggcgccgccc acggatgtcg cggacccgtc cgatcgctgc tctccaggaa
      241 gcccctgaac cacgatccca cccaggaaga gcgatgcatc cccagacccg acttgcgctc
      301 gcaggccaga agaccgaccg gagcttcggc agcgtggcgc tgcccattta ccagtccgcg
      361 atcttccgct tcggcgagga cggcgagaag ccggaattcg actattcgcg gagcggaaac
      421 ccgacacgcc tggccctcga gaggctcctc gccgagctcg agggcggcaa cgacgccgtg
      481 gccgtcggga gcggcatggc ggccatcgcc acgctgctgt ccctctacga cgccgacgcg
      541 cacgtcatct gctcgcacga ctgctacgga ggcacggagc ggctgttcac ctggcttgcc
      601 ggccagggaa agctctcggt ctcgttcgtg gacctgaccg atccggcgaa cctggccgcc
      661 gccgtgcggc cgtcgacccg ggcggtctgg gtcgaaaccc cgtcgaatcc gctgctccgc
      721 atcgtcgacc tccgggcgat cgggctattc tgcgaggcca acgggctcga tttcatcgcc
      781 gacaatacgt tcctgtcccc cctgctccag cgtccgatcg aattcggggc ggactacgtc
      841 gtgcactcca cgaccaagta cctgaacggc cactccgacg tcgtgggggg cgcgatcgtc
      901 gcccggacgg cggaccaggc cgaggcgatc cgcttcgcct ccaatacctt gggcacgaca
      961 gcggtcccct tcgactcctg gctggtgctc aggggcctca agacgcttcc ggtcaggctt
     1021 cgccagcacg aggagaatgc gcaccgggtc gcccgattcc tggagcggca cgacggcgtc
     1081 cggcgcgtct tctatcccgg ccttgagacc cacgaggggc acgagctcgc cgcgcgccag
     1141 cagtccggct tcgggggcat ggtctcgttc gaggtggatt ccggccgcgt ggacgtccga
     1201 atccttctgc gctcgacgac cgtattcacg cttgccgagt cgctcggcgg agtggaatcc
     1261 ctcatcgaac agccgtccac gatgagccac gcctccatgc gcccggaagc ccgcctccag
     1321 gccggcatca ccgaccaggt ggtgcgcctg tccgtcggca tcgaacacgg ggacgacctc
     1381 gtcgacgacc tggcccgggc gttcgacgtc gcggccagga ttccagttcg tcgccggcag
     1441 aacgcagcgg ccgtccggat ctgagcccgg atcgccgcga acaagatgac tcatcgcgct
     1501 attccccgat cccgaaacgg cgatcctcgt tagacgggat tccagaccga cctgcgaccc
     1561 catgcgcatc gacctcaccg accacgtgat cctcgtaacc ggcgcgagcc gcggcatcgg
     1621 ccgcgagacc gcgcggcagc tcgccgccgc gggcgcgacc gtggccgtgc attacgggcg
     1681 gggggccgag gaggccgcgg cgctggcggc ggaaatcggg aacggttcgc gcgcgttcgg
     1741 agccgatctc gccgacgccg gcgagaccgt gaagctcttc cgggacgtcg tagccgccta
     1801 cgggcacgtg gacacgctgg tcaacaatgc ggggatcgcg ctgaatgtgc ccgtcgccgc
     1861 cgagttggac cactgggtgc gggagtggga cgccacgctc gacgtcaacg cacgggcctc
     1921 ggcgctcctg gcgcgggagg cggtcgcgca tttcctggac cgcggccggg gagggcgcat
     1981 catcttcatc gcgtcgcgcg cggccttccg gggagacacg cccgactacc tggcctacgc
     2041 ggcgtccaag ggcgccatgg tctccatggc ccgtaccctg gcgcgcggat acgggaagca
     2101 gggcatcaag gcgttcaccg tggcgcccgg tttcgtccgg accgacatgg cgcagaacgc
     2161 catcgacgaa tacggcgagg ggttcgtcgt gaacgacctc gccctggacc ggctcacgga
     2221 accggccgac gtggccccgt tcgtggtcct gctctcgagc ggactgtcgg accacgcgac
     2281 cggcgcaacc atcgacatca atgccggcag ctacgttcac tgacccgccc gtcaaggtgc
     2341 tcaagttcgg cggcacgtcc gtcggcgacc tggacgggct caagcgtgtg ctctcgatca
     2401 tccggggcga gctcgtcgcc taccgggcga tcgtcgtcgt gtcggccacg tccgggacga
     2461 cggacacgct cgtggacctg gcgcgcgtgg aaccggccgg gcgcgatgcg ctcgtggacg
     2521 gcatccgtga ccggcacgag gcgctcgcgc gcgacctgct cggggattcc gcccatgcca
     2581 cctacctgag gctgctccgg gagcggctcg acgagcttcg ccgcgtgctc gccgagcctg
     2641 atgcgccgga gcgcacggcc gccgtcctcg cgaccggcga gcgcctgtcg gctccgctcg
     2701 tcgccctggg cctggaacgg ctggggctcg aggccacggc cgtcgacgcc gtggacctga
     2761 tctcgaccga ctcggacttc ggcgcggcga cggtggacct cgccgccacg cggcggacgg
     2821 tcgaggcctg gttcgcgatc ctcccgccga acgtggtccc cgtcgtcacc gggttcatcg
     2881 ggcgctcgag ctgcggccgc accacggtgc tcggacgcgg aggcagcgac tacaccgcgt
     2941 ccctgctcgc cgaggcggtc tcggcgacca agctggaccg ctggaccgac gtcgacggcc
     3001 tgcacacggc cgatccccgg cggttcgacg gaacgggccg cttcctgtac ctcctgctcg
     3061 aggacgcctc gacctggaac gagacgagcc ggctcggcat gcatccgcag gccttcgaac
     3121 cggtccggcg ggcgtgcatc ccggtccatg tccgctccac gcgccatccg cagggtgacg
     3181 gcacgctcat cctcccgcgc cgcctggccg ggcacgtgcg cacgcactcc cgcaacgcca
     3241 tgaagcgggc ggcccatcgc gagcgcggct ccgaggcgca gagcgaatga tgaacggcaa
     3301 gctgagggca ggcatcctgg gcgccaccgg ggcggtcggg cagaagttcg tcgagctgct
     3361 cgccgagcac ccctggttcg aggtgacggc gctcgcggcg tcggagcggt cggcgggtcg
     3421 gcgttaccgc gacgtcgtcc gctggatcgg gacgggccgc gtgcccgacg ccgtgggcga
     3481 cctcgaggtg cggccctccg aggcgccgct cgagtgcgac ttcgtcttct ccgggctcga
     3541 cgcatccgtc gcgggcgatg tcgagaccgc gttcgccgcc gccggatacc ccgtgatctc
     3601 gaacgcccgc aaccatcgca tggacgcgga cgtccctctc ctcgtcccgg aagtgaatcc
     3661 cgaccacgtg gcgctcgtcg accggcaggc gttcggcggc ggcggcttca tcgtcacgaa
     3721 cccgaactgc tccacggtcg ggcttacgat ggcgctcgcg cccctgcacc gggcgttcgg
     3781 cgtccgggac gtggcggtga ccacgttcca ggcgctctcc ggcgcgggct atcccggcgt
     3841 ggcgagcctg gacgccgtcg gaaacgtggt gcccttcatc ggcggcgagg aagccaagtt
     3901 ggtggccgag ccgcgccgga tcctgggcac gctcgcgccc ggcggcgtag aggacgcgga
     3961 catcgccatc agcccccagt gcaaccgcgt gccggtcatc gacgggcacc tggaatgcgt
     4021 ctccgtccgg ttcgagagga agccggactc gatcgccgag gtggccgagt gcctgcgcgg
     4081 cttcgcatcg cccgacgtcg tcgtcggcct gccgtccgcc ccggccctcc cggtgcaggt
     4141 gctggacgcg gccgacgcgc cccagccccg ccgcgacgtg atggcgggac gcggcatgac
     4201 cgtgtccgtg ggacgcgtgc gcgcctgcga ggtgctcgac gccaagttcg tgctcctggt
     4261 gcacaacacg atccgcgggg cggccggcgg ggccatcctc aacgcggagc tcctggtcag
     4321 gacaggctat ctgaagcctc gatcgtagcc ccgtccgcgg cggccgtcgc gaagagcatc
     4381 ggctcgagac cgctccgcgt ggcgtatcgc cgggagacgg acgaagtgaa cgcctcggcc
     4441 gcgtcctcgg cgacgagcgc caccgcgcat cccccgaagc ccgctccggt catccgcgcg
     4501 ccgaagcaac cgtcggtctc gcgcgcgagc tcggccatga ggtcgagtcg ggaggtcgag
     4561 acctcgaaat cgtcccgcag gctctcgtgg ctctcgtcca tgagctcgcc gaggcgttcc
     4621 gggtcggagg tgccggccgc ctccagcgtg cgctcgttct cggtaaccac gtgtcgggcc
     4681 cgcctgcgga tggtgtcgcc gagcccgccc tccctcgcca ggaaatcccg gagcgacacg
     4741 tcgcgcagcg cccgcacgcc cagccgccgg gcggcctcct cgcaactggc ccgccgctcg
     4801 ttgtactccg agtccacgag ccctcggcgc gtggcggtgt ccatcactac ggccacggtg
     4861 ccggccggca gcgtgacggg ctcggtctcg agcgagcggc agtcgatgag gagcgcggtg
     4921 ccctcgcgcc cgccggccga gagcatctgg tccatgatcc cgcagttcat cccgacccat
     4981 cggttctcgg cctcctgtcc gagccgcgcc atgcggtcgg cgcgccaggg ctcgcccggg
     5041 agggcgggcg ccgcgcgcag cgtggcgatc tcgagggccg cgctcgacga gcggccggcc
     5101 ccccccggca cgtcgcccgc caccccgccc tcccac
//
LOCUS       DBNHQZ010000025        17893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2579821, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000025 DBNHQZ010000000
VERSION     DBNHQZ010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2579821"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="queG"
                     /locus_tag="AC16YU_00565"
     CDS             <1..714
                     /gene="queG"
                     /locus_tag="AC16YU_00565"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR6063741.1"
                     /translation="DYRPAAEPRGDAGFGRISRYAWGDDYHEVMKERLAQLFDWLDRE
                     AGGAGGRVFVDSAPVMDKAWARRAGLGWIGKHTNLISRSRGSWFFLGEMIVDVPLAPD
                     GPVPDHCGTCTRCIDACPTGAIDRPYELDAARCISYLTIEHRPDDIEPELARGMGNWI
                     FGCDVCQEVCPWNSFSRAGAEPRYAPRDGMADTPLDEWEELDLEAFRVRFRHNPVKRA
                     KYEGFRRNVRIARENRDRA"
     gene            complement(737..2158)
                     /locus_tag="AC16YU_00570"
     CDS             complement(737..2158)
                     /locus_tag="AC16YU_00570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6063742.1"
                     /translation="MADLPIDAFPDRTGGVTLLAQPQEFAFADVAPDGSLRYVSPFGE
                     ARWGWSEGAMLPDEMLFMLAELAPGASWELPFMQGGLYVNGIRRGESDGWIVVGYEGR
                     PVAEDPENEPLDALVRRMPIAALRMQVDGTVVFANPAFARMTGYPMDEVVGYRFWTRV
                     IVEDDAPAFEEALRHVTDAEGSRVRFAYRTAQGGIQHVDMHLVLNEAYLVEAVVLDRP
                     VDSDDALLLHARLDAEQATRQKTAFIAMLSHEIRTPLGSIRGYADLLAREVADLEAAG
                     TTIPPTVREFVDSIEDRAGRLVDLVRDLLELSAIEMGHVSVENVPVRLRAVLREVIPT
                     ITDELERKRLTLAVDVPGDPAVLTDARRLELVLGRLLSNALKFTAEGSITIRAREEDG
                     SLAIEVQDTGIGISEAFRRRMFAPFNQEDDWLNRGHDGIGLGLTLVQRVVELLGGRLE
                     VDSREQAGSTFRVVLPAATVDLD"
     gene            complement(2229..3497)
                     /locus_tag="AC16YU_00575"
     CDS             complement(2229..3497)
                     /locus_tag="AC16YU_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6063743.1"
                     /translation="MDDAAQPVGYVALVRGNPNYRRLWLGSVVSLLGDWFNTIALYAL
                     VLQLSGSAVAIGAVFITKMLPWALVSPVAGLIVDRYDRRRLMIGADLVRAVIVLGFIL
                     IDEPGEVPLIYVLTAAQVVVGSVFPPAQSASIPNITTRQELLTANALSAATWSTILAV
                     GAALGGLATEWLGLRTVFVLDSLSYLWSAWFITRTVIPQSTERPSGRVSVGAAAREVL
                     DGWRFMRSRPEVGRMAFAKAAWAAAGGALVYMLTLIGEAIAPTSAAFAIGLLFSARGL
                     GTGIGPIVVRMTARDRGLWPAIIGACIGVTGVSYFLVGLVGFTYAVVTFVVIAHATSG
                     ANWVLSTVLLQERTEDRWRGRVFATEWLLLMATEAASILAASLLMDTGVLGLKEAILL
                     FAGLETLAGALWVAFVVPRERRWAAAVATA"
     gene            complement(3503..5065)
                     /gene="lysS"
                     /locus_tag="AC16YU_00580"
     CDS             complement(3503..5065)
                     /gene="lysS"
                     /locus_tag="AC16YU_00580"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6063744.1"
                     /translation="MTRERSEQELRRREERAALEAAGIDPYPYEWAVTHRSEAILAEF
                     DDARHQPGEDAPAPEPLRVSVAGRIMTRRIMGKAAFFHLQDAYGQIQVYVRRDDLPEG
                     FYNDVFRKLLDIGDIVGVEGFAFRTKMGEVSVHAERMEVLAKALRPLPVVKEQDGRVY
                     NEVRDKEFRYRQRYVDLVVNPQVREVFRERARMIATLRRFLDQRGYIEVETPVLQPLY
                     GGAAARPFTTHHNALDVGLFLRIADELYLKRLIVGGFDGVYEISKDFRNEGLSRFHNP
                     EFTMLELYVAYRDYRWMMELVETMLEEVAITMHGSTDVRAGDVTVSFARPWPRIPIFE
                     AIREHTGHDLEGMDRGGLADVGRKLGLDVTSEMGAGKIIDVIFGEFVEPELIQPTFVT
                     DYPVELSPLAKRHREKPGLVERFEAIIGGKEICNAFSELNDPDDQRARFEEQARLRAA
                     GDTEAMQVDEDYLRALEYGMPPTAGLGVGIDRLAMLMTGQESIRDVILFPLLRPEQEA
                     PDGDPGGTPVDS"
     gene            complement(5097..5798)
                     /locus_tag="AC16YU_00585"
     CDS             complement(5097..5798)
                     /locus_tag="AC16YU_00585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6063745.1"
                     /translation="MNRMLTAVLLSCLAISCSPPDTSFYEISTPKGRMVVRLYDETPQ
                     HRDNFRRLADEGFYDGTLFHRVIARFMIQGGDPNSKDEDPMNDGQGGPGYRIPAEFVP
                     GLFHKRGALAAARDNNPERASSGSQFYIVHGAVLADSLLPGLEERISRQTGTEFRFSP
                     EAREAYTTLGGAPWLDGQYTVFGELVEGFEVLDSIAATPTPNVLGHPAPPGIGERPVE
                     DITMTVRPLPDYGRN"
     gene            complement(5795..6190)
                     /locus_tag="AC16YU_00590"
     CDS             complement(5795..6190)
                     /locus_tag="AC16YU_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063746.1"
                     /translation="MTRPAASWSAVLDRVDLELEADWARFADWWIRRYDRDPTVESAL
                     FLVGLQALGRGAPRRMSKEEKQDAIMEGTYRVLETVGMYRRVDDATWERTGDLPVLST
                     SEQEKLLRAGIIRYFEPVMSETIDRKPSP"
     gene            complement(6187..6699)
                     /locus_tag="AC16YU_00595"
     CDS             complement(6187..6699)
                     /locus_tag="AC16YU_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063747.1"
                     /translation="MFGWDTDRPEDSRLPMVSDHESAERIERLQLAADRKAPAFQSVL
                     AVVVSDLESVREAESGVVGAAISLAHREKVGAMLEETESALSALHRLAVRPLAVTGPK
                     SNEDARGSWWYALADATHTLEAGIDRISSLVGSQPRRSGVRDLASAAVRLMRSHQRML
                     LDEAGRWIEG"
     gene            complement(6808..7758)
                     /locus_tag="AC16YU_00600"
     CDS             complement(6808..7758)
                     /locus_tag="AC16YU_00600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022187.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR6063748.1"
                     /translation="MKRRKEIRQDRFVMIGAGAAASALGPALRAAGYDAAAVIGRDPE
                     RAARLARSLGAATVHFGLEPRTGTWADLPDAELYSICVPDRVIPEIAEALAQLSLDWK
                     GRRVLHVSGALDASPLAPVAARGAVTLAFHPVQTFTLRTDAGSFDGISVGVSGNGAGR
                     DAGLDLAARMGLRAVIVEDADRPLYHLATALVSNGAVTLSAMAHEVLAACGIGGEESR
                     RLLAPLVTASARNLASGEPGSVLTGPVVRGDAQTVERHLACLAAGGSHLVPAVASLTT
                     ETVRLAVRSGRLDEDAAGRILEIISRFLDAEGHSPQETEP"
     gene            complement(7777..8532)
                     /gene="amrB"
                     /locus_tag="AC16YU_00605"
     CDS             complement(7777..8532)
                     /gene="amrB"
                     /locus_tag="AC16YU_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6063749.1"
                     /translation="MPSSQSVSKDLAHPASRLLRAAVRPTFEGELFVLVVPRTGGADA
                     LAARVFKAAEGAAYRSVIVVASARQEMDGRIHLSRRESYPSAVGSIPVDLKSVDELCD
                     EDDDIYADDRGLYADDGIGDQLPWLRAALGEFALVPVVMGDESPDYCRELGAAVGEVM
                     YNQPTLLVACVDVLEASAGELKAFGDALAANDVGALMRAVMSERIRLDGAGPLLAALL
                     AADRRGARRVTVLELTAPAGAVPGRLGALITRA"
     gene            complement(8560..9258)
                     /gene="bioD"
                     /locus_tag="AC16YU_00610"
     CDS             complement(8560..9258)
                     /gene="bioD"
                     /locus_tag="AC16YU_00610"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR6063750.1"
                     /translation="MAKGLFLVGTARGVGKTLLGAGLVGVLRRMGVDATMMTPVSTGG
                     AVESAFEMLRGLGVDDPRRLVNPFAFETMASPLVASRIESKAIEPRTILEAFLELGES
                     REFVVVEGGGVLVPIARQYFMIDLLEDFGLPSIIIGRSGRGTLNHCLLTQRMMMVRGD
                     HPEGFILNGYGQYGDGFAESLNPDVLAELAAPTPVLGTLEWRPAYQEDLGAVIGALET
                     LPDLVALLRRSAGA"
     gene            complement(9417..10121)
                     /locus_tag="AC16YU_00615"
     CDS             complement(9417..10121)
                     /locus_tag="AC16YU_00615"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR6063751.1"
                     /translation="MKAPRSPQQIIDAQVHAWSLEQRRPSGSRAAHHPIITVSRSFGA
                     QGDTLGRILGEMTGFDVWDRDLVNAVADAAGGDVRIMESLDERRRRAIDDAVRGFLSG
                     IDHTNTQYFRALARVIRTIAEHGRAVVVGRGANYFVSADRALRLRVTAPLAWRVDAYA
                     ERMGITRAKARETVRERDVERADFVSHFFKRDIADPTEYDLVLNAASLSPNAMARMAI
                     DAYEVRFGLHVAHETA"
     gene            complement(10252..10944)
                     /locus_tag="AC16YU_00620"
     CDS             complement(10252..10944)
                     /locus_tag="AC16YU_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063752.1"
                     /translation="MSILASARTGLAVVASAVVFAGCGSSNRLARYDFYDASIYADAP
                     LAAPPDVYTDLAWSTEPRQGDSPILAAIRIGSAVVKEVGAEEARHKLLEAEERVDVPA
                     RIADAAMERVTRLLRANEVGDLREADFLVEILVERQGIFAADPVTGSLQYGVDGRVRM
                     IHLDSGRRVWERKFREREVFARSVGGSAGAIVSGVQLSEMSVEEMAEALERLSYRMGS
                     TVARYMGEDLDG"
     gene            11233..13014
                     /locus_tag="AC16YU_00625"
     CDS             11233..13014
                     /locus_tag="AC16YU_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6063753.1"
                     /translation="MTFDQLSVFGVLIASLALFLWNRLRYDVVAVLVLLGAAILGLVP
                     PERVFSGFGHPAVITVAGVLVISRALVNAGVVDSLARRLTRVGDRPALQVGTLTGIVA
                     LTSGFMNNVGALALLMPVAIWMSRRSGRSPALLLMPLAFGSLLGGMLTLIGTPPNIII
                     AAYREHATGTAFGMFDFLPVGAAVTLAGVAFITLLGWRMTPRRTDDGGGGDLFDIGGY
                     LAEVLVPPDSTFVGLTLRHALAELGDDAEVVIIGLVRKGEMQEMPSVFEILHGGDILV
                     IEAGSEDLDTFIHRTGFEIEAGTAQDGSPDPELSLHLAEIIVTPSSSLVGRTAVRVDL
                     RNRYGLNLVAVARQGHRLNERLGNVRFVAGDILLVQGEASTLKSRLAELGCLPLASRG
                     LRIGKPRNVILSAAIFGAVLAAVSLDVVPPATAMIAGAVAMVLVGLIGKDESYRSIDM
                     PVIVLLAAMLPIGEALETTGGSRLIADALLAGSASLPPAMMLAMLMTAIMLLSNVINN
                     AAAAVLSAPIAIDLAHGLGASVDPFLMAVAVASSSAFLTPIGHQSNALVMAPGGYRFG
                     DYWRMGLPLSILIVAIAVPMILWVWPL"
     gene            13123..13275
                     /locus_tag="AC16YU_00630"
     CDS             13123..13275
                     /locus_tag="AC16YU_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063754.1"
                     /translation="MTRVSYSRRDRAAGLGDGLLSGAVGTGPVLAGVLLGSRRVTGWE
                     RAPAPR"
     gene            complement(13342..13686)
                     /locus_tag="AC16YU_00635"
     CDS             complement(13342..13686)
                     /locus_tag="AC16YU_00635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6063755.1"
                     /translation="MSWNLRLAVREGQLENFRALMNEMVESTRQEPGALGYEWFLSED
                     GSACHIQERFTDSDAVMVHLAAFGSRFAERFLGCVQPTGFDVYGSPSAEVRAVVAGFG
                     AAHLGAFGGFVR"
     gene            13903..14589
                     /locus_tag="AC16YU_00640"
     CDS             13903..14589
                     /locus_tag="AC16YU_00640"
                     /inference="COORDINATES: protein motif:HMM:NF053222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRA domain-containing endonuclease"
                     /protein_id="HIR6063756.1"
                     /translation="MKHINLDRLPKGELIRRADLHTAYGGSKKADVAPSRKAGGLFLF
                     SADAGAPYDIRDGWSAPNEYELCVVVDDPGHSWDTASRSIRDHQKAGRRMYLFRTTSP
                     EMCRYLGRVELRGYRTEQVTDRARRTRTAVIFTLRESRLVLDMSASDHSLVDSAMKQV
                     AERLRQEGSDFVGPVPLPSDRDASENGDGAPGRMHRRSIYVRFPEASTIAALTELPLP
                     SGIEIELRQE"
     gene            14784..15095
                     /locus_tag="AC16YU_00645"
     CDS             14784..15095
                     /locus_tag="AC16YU_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063757.1"
                     /translation="MPFGPFGFVIAIIMVVFGFVAFTVKLNNDYKLRREELQSGREGS
                     SLGSGELRQLIREAVADAIVPVEERLELIERQMRQLPEPRPAPPRQPGPGDEPTRGPL
                     P"
     gene            15092..16462
                     /locus_tag="AC16YU_00650"
     CDS             15092..16462
                     /locus_tag="AC16YU_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR6063758.1"
                     /translation="MTLPLSRRRFLRTTGAAGLGAALVGLPRCGRAVGAGTVRVLSIG
                     VVGTIGGWDREQIADHPRAEIVGLCDVDAAYLAQAAADHPDAFTCRDYREAFARYADR
                     FDAVIVATPDHTHAPIMLTALAHDKHVYGQKPLVHQLEEITLIERAMRAKPGLVTQVG
                     NQRMVNPGRRAAVEILRSGALGRAVEAHIWTGGPDDSIYFNYGRVLSDPLPPPPGLDW
                     DLWLGPCEPRPYRDLLVPAKWRSWWDFGTGGIGDWGVHLLDVIFFAFDELTTPVSVVA
                     HTPEPAGTFHTFPTRTTTTYAVDSDRFAGSIFPIHYNDVAQAPPRESLGLPAGGWMGT
                     NMTVVVCEGGVMVLAPEGELEIWRDGRMTPGLEMPGLPEFPELNHWHAWVDTILGVET
                     ELRTPFVDGARITEAALLAVKATRFPGQELLWDKASLSFTNHPEATAAIVRRDYREGF
                     APPVVG"
     gene            complement(16675..>17893)
                     /locus_tag="AC16YU_00655"
     CDS             complement(16675..>17893)
                     /locus_tag="AC16YU_00655"
                     /inference="COORDINATES: protein motif:HMM:NF051532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6063759.1"
                     /translation="TIGVVRDTLFVYSDDPIEPVRGVPFQVTFRDWFAVADNDDPASY
                     EESGAWNTSVTQAYGPSSRYAGLPSAWTATASFTTTVQQGGVFDASYFVPSTVNAAVR
                     ADYLVSVDGVRIDSVRVDQNQGSGAWRPLGLYELEAGSVVRVTVRASDPDQSGRVLRA
                     DAFRLSRMGDELRTAVLDDADAEHYEEAGTWLSSNSQAYGTSSRYAGQGSGATATFRS
                     VAEVTGVHALHEIVPTTVNASTQAQYRVLRNGELVESLVIDQNQGSGAWRALGAWLFS
                     TGDEIAVEVSEAEGSTGVLRADAIRVDFGAASDVAVERDLPLDGWSLGPNWPNPFGGR
                     TRFAYSVGAPGRVVVELYDVLGRRVGTVVDEVLQTGTHEAEFDASGLSSGVYLARLRG
                     PGFHASRTWIVAR"
BASE COUNT         2725 a         6395 c         6081 g         2692 t
ORIGIN      
        1 gactaccggc ccgccgccga gccgcgcggg gatgccgggt tcggccggat cagccgctac
       61 gcgtggggag acgactacca cgaggtgatg aaggagcgcc tcgcccagct tttcgactgg
      121 cttgaccgcg aggccggggg cgccggcggg cgggtcttcg tggactcggc cccggtgatg
      181 gacaaggcgt gggcgcgccg cgcggggctc ggctggatcg gcaagcacac gaacctcatt
      241 tcccggagcc ggggctcgtg gttcttcctg ggcgagatga tcgtggacgt gccgctcgcc
      301 cccgacgggc ccgttcccga ccactgcggg acatgcacgc ggtgcatcga cgcctgcccg
      361 acaggggcga tcgaccgtcc gtacgagctc gacgccgccc gctgcatctc ctacctgacg
      421 atcgagcatc gcccggacga catcgagccg gagctcgccc gcggcatggg caactggatc
      481 ttcggctgcg acgtgtgcca ggaggtctgc ccctggaact cgttcagccg cgcaggcgcc
      541 gagccgcgct acgccccgcg ggatggcatg gcggatacgc cgctcgacga atgggaggag
      601 ctcgacctcg aggccttccg ggtgcggttc cggcacaacc ccgtcaagcg ggcgaagtac
      661 gagggcttcc ggcgcaacgt gcggatcgca cgggagaacc gggaccgggc ctgacgggag
      721 cgcccttgcc gtgcggctag tcgagatcga ccgtcgctgc cggcagcacc acgcggaatg
      781 tggacccggc ctgctcgcgg ctgtcgacct cgagccggcc gccgagcagc tccacgacac
      841 gctgcacgag cgtgaggccg aggccgattc cgtcgtggcc gcggttcagc cagtcgtcct
      901 cctggttgaa gggggcgaac atccggcggc ggaacgcctc ggagataccg atgccggtgt
      961 cctggacctc tatcgccagg cttccgtcct cttcgcgagc ccggatggtg atcgagcctt
     1021 cggccgtgaa cttcagcgcg ttcgagagca gacggcccag gaccagctcc agccgccggg
     1081 cgtccgtgag cacggcggga tcgccgggga cgtccacggc gagcgtgagg cgcttccgct
     1141 cgagctcgtc cgtgatcgtc gggatcactt cacgcagcac cgcgcgcagt cgaaccggga
     1201 cattctccac ggagacgtgg cccatctcga tcgcggagag ctccagcagg tcccgcacca
     1261 ggtccaccag gcggccggca cggtcctcga tcgagtcgac gaactcccgc acggtcggag
     1321 ggatcgtcgt ccccgccgcc tccaggtcgg caacctcccg ggcgaggagg tcggcatagc
     1381 cccggatgga gccgagcggc gtccggatct cgtggctgag catggcgatg aaggcggtct
     1441 tctggcgggt cgcctgctcg gcatcgagcc tggcatgcag cagcagggcg tcgtcggaat
     1501 cgacggggcg gtccagcacg accgcctcga cgaggtaggc ctcgttcagg accaggtgca
     1561 tgtccacgtg ctgtatgccc ccttgcgccg tccggtaggc gaagcggaca cgggagccct
     1621 ccgcgtccgt cacgtggcgc agcgcctcct cgaatgcggg cgcgtcgtcc tcgacaatca
     1681 cgcgcgtcca gaaacggtat ccgacgacct cgtccatcgg gtagccggtc atgcgggcga
     1741 acgcgggatt cgcgaatacg accgtcccgt ccacctgcat ccggagcgcg gcgatcggca
     1801 tgcggcggac gagcgcgtcg agcggctcgt tctcgggatc ctccgcgacg gggcggccct
     1861 cgtagcccac cacgatccag ccgtcggact cgccgcgccg gattccgttc acatacagcc
     1921 cgccctgcat gaagggcagc tcccaggacg cgcccggcgc gagctccgcg agcatgaaga
     1981 gcatctcgtc cggcaacatg gcgccctcgg accatcccca gcgtgcctcg ccgaacgggc
     2041 tcacgtagcg cagcgacccg tcaggggcca cgtcggcgaa agcgaattcc tggggctgtg
     2101 cgagcagggt gacgccgccg gtccggtccg gaaaggcatc gatggggagg tcggccacgt
     2161 gtttcaaacc gggattgctg tctcggcgga cgcaccaatc gaatacagtg ccaaacgtcc
     2221 gaccggcctc aggccgtggc gacggcggcg gcccagcgcc gctccctggg cacaacgaag
     2281 gccacccaga gggcacctgc gagggtttcc agcccggcaa acagcaggat agcctccttg
     2341 agtccgagca ccccggtgtc cataaggaga ctcgccgcga gaatggaggc cgcctccgtc
     2401 gccatgagca gcagccactc ggtggcgaac acgcggccgc gccagcggtc ctctgtgcgc
     2461 tcctgcagga ggacggtcga gagcacccag tttgcgccgc tcgtcgcgtg ggcgatcacc
     2521 acgaaggtga cgaccgcgta cgtgaagccg acgagtccca ccaggaagta gctcacgccc
     2581 gtcacgccga tgcaggcgcc gatgatcgcg ggccagaggc cgcggtcccg cgcggtcatg
     2641 cggacgacga tggggccgat ccccgtcccg agcccgcgcg ccgagaacag gagtccgatg
     2701 gcgaaggccg cgctcgtggg cgcgatcgcc tcgccgatga gcgtgagcat gtagacgagg
     2761 gcgcctcccg cggccgccca ggcggccttc gcgaacgcca tgcgcccgac ctccgggcgg
     2821 ctccgcatga agcgccatcc gtcgaggacc tcgcgcgcgg cggcgcccac gctcacgcgg
     2881 cccgagggcc gctcggtgga ctgcggaatc accgtccgcg tgatgaacca cgccgaccac
     2941 aggtacgaca ggctgtcgag cacgaaaacg gtccggagcc cgagccactc tgtggccagc
     3001 ccgccgaggg ccgcgccgac ggccaggatc gtggaccaag tggccgccga gagggcattc
     3061 gcggtcagga gctcctgccg ggtggtgatg ttcgggatcg atgcgctctg cgccggcggg
     3121 aacaccgatc ccaccacgac ctgggccgcc gtcaggacgt agatgagcgg cacttcgccc
     3181 ggctcgtcga tcaggatgaa gcccaggacg atgaccgcgc gcacgaggtc ggccccgatc
     3241 atgagccggc gacggtcgta ccggtccacg atgagacccg cgacgggcga gacgagcgcc
     3301 cacgggagca tcttcgtgat gaacaccgcc ccgatcgcga ccgcggaccc cgaaagctgg
     3361 aggaccagcg cgtagagcgc gatcgtgttg aaccagtcgc cgagcagcga caccacgctt
     3421 cccagccaca gcctgcggta gttcgggttg ccccggacca gggccacgta cccgacaggc
     3481 tgcgccgcgt cgtccatcgt cgtcaggagt cgactggtgt ccccccggga tccccgtccg
     3541 gagcctcctg ctccggtcgc aggagcggaa acaggatcac gtcgcggatc gattcctgcc
     3601 ccgtcatcag catggccagc cggtcgatgc ccacgccgag cccggccgtc ggcggcatgc
     3661 cgtactcgag cgcgcgaagg tagtcctcgt ccacctgcat cgcctccgtg tcgccggccg
     3721 cccggaggcg cgcctgctcc tcgaagcgag cccgctggtc gtcggggtcg ttcagctcgc
     3781 tgaacgcgtt gcagatctcc ttgccgccga tgatcgcctc gaagcgctcc accaggcccg
     3841 gcttctcgcg gtgccgcttg gccagcgggc taagctccac ggggtagtcc gtgacgaagg
     3901 tgggctggat gagctccggc tccacgaact cgccgaagat cacgtcgatg atcttgcccg
     3961 cgcccatctc cgacgtgacg tccagcccca gcttccgccc cacgtcggcc aggccgccgc
     4021 ggtccatgcc ctccaggtcg tggcccgtgt gctcccggat ggcctcgaag atcgggatgc
     4081 ggggccaggg cctcgcgaac gagaccgtca cgtcgcccgc gcggacgtcg gtggagccgt
     4141 gcatcgtgat cgccacctcc tcgagcatgg tctccacgag ctccatcatc cagcggtagt
     4201 cgcggtaggc gacgtacagc tccagcatcg tgaattccgg attgtggaag cggctcagcc
     4261 cctcgttccg gaagtccttc gagatctcgt agacgccgtc gaacccccca acgatcaggc
     4321 gcttcaggta cagctcgtcc gcgatccgca ggaagagccc gacgtcgagc gcgttgtggt
     4381 gcgtggtgaa gggccgcgcc gcggccccgc cgtagagcgg ctgcaggacg ggcgtctcga
     4441 cctcgatgta gccgcgctgg tccaggaaac gcctcagggt ggcaatcatg cgggcccgct
     4501 cgcggaagac ctcgcgcacc tgcgggttca cgaccaggtc cacgtaccgc tggcgatagc
     4561 ggaattcctt gtcccggacc tcgttgtaca cgcggccgtc ctgctccttg accacgggca
     4621 gcggacgcag ggccttggcc aggacctcca tgcgctcggc atgcacggag acctcgccca
     4681 tcttcgtccg gaacgcgaac ccttccaccc cgacgatgtc gccgatgtcc agcagcttcc
     4741 tgaagacgtc gttgtagaag ccctcgggca ggtcgtcccg gcggacgtac acctggatct
     4801 gcccgtacgc gtcctgcagg tggaagaagg ccgccttgcc catgatccgc ctggtcatga
     4861 tccgcccggc gacggacacg cgcaggggct cgggcgcggg cgcatcctcg cccggctggt
     4921 gcctcgcgtc gtcgaactcg gcgaggatgg cctccgagcg gtgggtcacg gcccactcgt
     4981 acgggtacgg atcgatcccc gccgcctcga gggcggcccg ctcctcgcgg cgtctcagtt
     5041 cctgttcgct gcgctcgcgg gtcatggaca ggcagggaga tgggtcggat ggcccgtcag
     5101 ttacggccgt agtccggcag ggggcgcacg gtcatcgtga tgtcctcgac cggacgctcc
     5161 ccgatgcccg gcggcgccgg gtggcccagg acgttcggcg tgggagtggc cgcgatgctg
     5221 tccagcacct cgaagccctc gacgagctca ccgaacacgg tgtactgccc gtcgagccac
     5281 ggggcgccgc cgagcgtcgt gtacgcctcg cgcgcctcgg gagagaaccg gaattccgta
     5341 cccgtctggc gactgatccg ctcctcgaga ccgggcagaa gggaatcggc cagtaccgcg
     5401 ccatgcacga tgtagaactg gctgccgctc gaggcgcgct cgggattgtt gtcgcgggcg
     5461 gccgcgaggg cgccccgctt gtggaagagg cccggcacga actcggccgg aatccggtac
     5521 ccggggccgc cctggccgtc gttcatcggg tcctcgtcct tcgagttcgg gtcgccaccc
     5581 tggatcatga accgggcgat tacgcggtgg aagagcgtcc cgtcatagaa cccttcgtcc
     5641 gcgagccggc ggaagttgtc gcggtgctgc ggggtctcgt cgtacaggcg cacgaccatc
     5701 cgccccttgg gggtcgaaat ctcgtagaag gaggtgtcag gcggcgagca ggagatcgcg
     5761 aggcatgaca ggaggacggc ggtgagcatt cgattcatgg gctgggtttt cggtcgatgg
     5821 tctccgacat cacgggctcg aaataccgga tgataccggc ccgaagcagc ttttcctgct
     5881 cggaagtgga cagcacgggc aaatcgccgg tccgttccca cgtggcgtcg tccacccggc
     5941 ggtacatgcc gaccgtctcg agcacgcggt acgtgccctc catgatcgcg tcctgcttct
     6001 cctctttcga catgcggcgg ggcgcgccgc gcccgagcgc ctgcaggcct acgagaaaga
     6061 gcgcggattc cacggtcgga tcgcgatcgt accggcggat ccaccagtcg gcgaaacggg
     6121 cccagtccgc ctcgagctcc aggtcgaccc ggtccaggac ggcggaccag gaggcggccg
     6181 gccgggtcat ccctcgatcc agcggcccgc ctcgtccagc agcatccttt gatggctgcg
     6241 catcagccgg acggccgcgc tcgccaggtc ccgcacgccg ctccggcgcg gctgggatcc
     6301 gacgagagag gagatccggt cgattccggc ctccagcgtg tgcgtggcgt cggcgagagc
     6361 gtaccaccac gagcccctgg catcctcgtt cgacttcggc cccgtgacgg ccaggggacg
     6421 gacggcgagc cggtggagcg cggacagggc ggattcggtc tcctcgagca tggcgcccac
     6481 cttctcgcgg tgcgccagcg aaatcgccgc tccgaccacg ccgctctcgg cctcgcgcac
     6541 ggactcgagg tccgagacga cgacggccag taccgactgg aaggccggag ccttccgatc
     6601 ggctgcgagc tgcagccgct cgatgcgttc cgccgactcg tgatcactca ccatgggaag
     6661 cctggagtcc tccggacggt ccgtatccca cccgaacatg cggcgggggg ggttctctcg
     6721 acgaagtcgg ctcaagcggg ccgataccat aggcgaccgg gacgaacccg ggttcaggaa
     6781 gggcggcggg catatgcagg ggtacgttca aggttccgtt tcttgcggcg agtgcccctc
     6841 agcgtccaga aaacgcgata tgatttcaag aatccttcca gcggcatcct cgtccagtcg
     6901 gcccgagcgc accgccaggc ggaccgtttc ggtcgtgagc gaggcgacgg cagggaccag
     6961 gtgagacccg ccggcggcga ggcaggccag gtgacgctcc acggtctgcg cgtctccccg
     7021 cacgaccggg ccggtgagca ccgacccggg ctctccagac gcgaggttcc tggcggaggc
     7081 cgtgacgagt ggcgccagca gacgacggct ctcctcgccg ccaattccgc acgccgcgag
     7141 cacctcgtgc gccatcgcgg agagcgtgac cgcaccgttc gacaccaggg ccgtcgccag
     7201 gtggtacagc ggccggtccg cgtcctcgac gatcaccgcg cgcaatccca tccgggccgc
     7261 aaggtccagc cccgcatccc ggccggcacc gttcccggaa acgcccacgc tgatgccgtc
     7321 gaacgagccc gcgtccgtcc gcagggtgaa cgtctgaaca gggtggaagg ccagggtgac
     7381 ggctccccgg gccgcgacgg gcgcgagggg tgaggcgtcg agcgcgcccg acacgtgcag
     7441 cacccgcctg cccttccagt cgagggacag ctgcgcgagc gcctcggcga tttccgggat
     7501 cacccggtcg ggcacgcaga tgctgtacaa ttccgcatcc ggcaggtccg cccatgtccc
     7561 ggtgcggggc tccagcccga agtggaccgt cgcggcgccc agcgagcggg cgagccgcgc
     7621 cgcccgttcc ggatccctcc cgatgaccgc cgccgcatcg tagcccgcgg cccggagcgc
     7681 cggcccgagg gccgacgccg cggcgcccgc gccgatcatg acgaaccggt cctgacggat
     7741 ctccttccgg cgtttcatgg atggtcggcc cgggggctat gcgcgggtga tgagcgcgcc
     7801 caggcgaccc ggcacggcgc ccgcgggagc cgtgagctcc aggacggtga cgcggcgcgc
     7861 ccccctccgg tcggccgcga gaagcgccgc caggagcggt ccggcgccat cgagccggat
     7921 ccgctccgac atgacggccc gcatcagggc gcccacgtcg ttcgcggcca gggcgtcgcc
     7981 gaaggccttc agctcgccgg ccgacgcctc cagcacgtcg acgcaggcca cgagcagcgt
     8041 cggctggttg tacatgacct cgccgaccgc cgccccgagc tcgcggcagt agtcgggcga
     8101 ctcgtcgccc atgacgaccg gcaccagcgc gaactcgccg agcgcggccc gcagccaggg
     8161 cagctggtcc ccgatgccgt cgtccgcgta caggcccctg tcgtcggcgt agatgtcgtc
     8221 atcctcgtca cagagctcgt ccaccgactt gaggtccacg ggaatgcttc ccacggccga
     8281 cgggtacgat tcgcggcggc tgaggtggat ccgcccgtcc atctcctgcc gggcggacgc
     8341 cacgacgatc accgaccgat acgcggcacc ctccgccgcc ttgaaaaccc gggccgcgag
     8401 ggcgtcggcg ccgccggtcc ggggaacgac gagcacgaac aactcgccct cgaaggtcgg
     8461 ccggaccgcc gcgcggagga gccgcgaggc cgggtgggcg aggtctttcg atacggattg
     8521 ggatgacggc atggcaccgt acgggatggt gaaacggttt caggcgccgg ccgagcggcg
     8581 gagcagcgcg acgaggtcgg gcagggtctc cagcgcgccg atcacggcgc cgaggtcttc
     8641 ctggtaggcg ggccgccact cgagcgtccc cagaaccggg gtcggcgccg cgagctcggc
     8701 gagcacgtcg gggttgagcg attcggcgaa tccgtcgccg tactgaccgt agccgttcag
     8761 gatgaatccc tccgggtggt ccccgcgcac catcatcatg cgctgggtga gcaggcagtg
     8821 gttgagcgtt ccccggccgg accggcctat gatgatcgac ggcaggccga aatcttcaag
     8881 gagatcgatc atgaagtact gccgggcgat cggcacgagc acgcctcccc cctcgaccac
     8941 gacgaactcc cggctttcgc cgagctccag gaatgcctcc aggatcgtcc gcggctcgat
     9001 cgccttgctc tcgatgcggc tggcgaccag cggcgacgcc atcgtctcga acgcgaacgg
     9061 gttgaccagg cggcggggat cgtccacgcc caggccgcgc agcatctcga aggcgctctc
     9121 caccgcgccg cccgtcgaga cgggcgtcat catcgtggca tccaccccca tccgccgcag
     9181 gacgcccacc agtccggcgc ccagcagggt cttgccgacg ccgcgtgcgg tacccaccag
     9241 gaaaagaccc ttcgccatgc ggaatgtcgg gacggacgcg ccccgcggat tcggggcggt
     9301 caaagtaggc cgcttccccg ccggcttcaa gccgcggcct gcgggggctg ccgcgggcgt
     9361 gcggctgcgg cggcaggcga aggcgccgcg acgattccgg cccttgcgcg gccgattcag
     9421 gcggtctcgt gggccacgtg caggccgaac cgcacttcgt aggcgtcgat cgccatgcgc
     9481 gccatcgcgt tcggggagag cgaggccgcg ttcaggacca ggtcgtactc ggtggggtcg
     9541 gcgatgtcgc gcttgaagaa gtgcgatacg aaatccgccc tttccacgtc ccgttcgcga
     9601 acggtctccc gcgccttcgc gcgggtgatc cccatgcgct ccgcgtacgc gtccacccgc
     9661 cacgcgaggg gtgcggtcac ccggagccgg agcgccctgt cggccgagac gaagtaattg
     9721 gcgccgcgcc cgaccacgac cgcccggccg tgctcggcga tcgtccggat cacgcgggcg
     9781 agggcgcgga agtactgcgt gttcgtgtgg tcgattccgc tcaggaagcc gcggacggcg
     9841 tcgtcgatcg ccctgcgccg gcgctcgtcg agcgactcca tgatgcggac gtccccgccg
     9901 gccgcgtccg cgaccgcgtt cacgaggtcc cggtcccaga cgtcgaatcc ggtcatctcg
     9961 ccgagaatcc ttccgagggt gtcgccctgc gccccgaagg accgcgagac cgtgatgatc
    10021 ggatggtgcg ccgcccgcga tccgctcggc cgccgctgtt cgagcgacca cgcgtggacc
    10081 tgcgcatcga tgatctgctg aggagagcga ggagctttca tggcgtgccg gggcatgttc
    10141 agcatcgggt acagaataat gccccggcac aaggatgtct gtgcggaaat cccgcgaatg
    10201 gcggtcggcg aggcacggat cgttgtagcg gccgcgccgg cggaggcgcc cctacccgtc
    10261 gagatcctcg cccatgtagc gcgccacggt cgatcccatc cggtatgaaa gccgctcgag
    10321 cgcctcggcc atttcctcga cgctcatctc ggagagctgg acgcccgaga cgatcgcccc
    10381 cgcgcttcct cccacgctcc gcgcgaagac ttcccgctcg cggaacttgc gctcccacac
    10441 gcgtctgccc gagtccaggt ggatcatgcg aaccctgccg tccaccccgt actgcaggct
    10501 gcccgttacc ggatcggccg cgaagatgcc ctgccgctcc accaggatct cgacgaggaa
    10561 gtcggcctcg cgcaggtcgc ccacctcgtt tgcgcggagc aggcgcgtca cgcgctccat
    10621 ggcggcatcc gcgatgcggg ccggcacgtc cacccgctcc tcggcctcga gcagcttgtg
    10681 tcgggcttcc tcggccccga cctccttcac gacggcggat ccgatccgaa tggcggccag
    10741 gatcggcgag tcgccctgcc ggggctcggt cgaccacgcg aggtcggtgt acacgtcggg
    10801 cggcgcggcc agcggcgcgt cggcgtagat cgaggcgtcg tagaaatcgt agcgggccag
    10861 gcggttcgac gagccgcagc cggcgaaaac gaccgcggag gcgacgacgg cgaggcccgt
    10921 gcgggcggaa gcgaggatcg acatggcagt tcgggttcgg tgagttcctg gcggccgaag
    10981 gccagcgtac ccacgcggga tcggccgttc tcacgcgaat tccaatcata cgccgtgcca
    11041 atcgcgcccg gaagcaggca aatcgccgaa tccgagccgt gggtgtcccg aatcggctac
    11101 accggcgacc ggtccggatg acggccggcc cgagaaccca acccctccac ggcacgtacc
    11161 cgctcccggg cgaggagtgc gatccctggg cccgacccca cccgccgcgt gcctgccccc
    11221 gcacacatgg acgtgacttt cgaccagctg tccgtcttcg gcgtgctcat cgcctcgctc
    11281 gccctgttcc tctggaacag gctacggtac gacgtggtcg ccgtcctagt gctgctcggt
    11341 gcggcgatcc tggggctcgt gccgccggaa agggtgttct cgggattcgg ccacccggcc
    11401 gtgatcaccg tggccggagt gctcgtgatc agccgggccc tcgtgaacgc cggggtagtc
    11461 gacagcctgg cccggcggct gacccgcgtc ggcgatcggc ccgccctgca ggtcggcacg
    11521 ctgaccggca tcgtcgccct cacctccgga ttcatgaaca acgtcggggc gctcgccctg
    11581 ctcatgcccg tggccatctg gatgtccagg cggagtgggc gctcccctgc gctgctcctg
    11641 atgccgctcg cgttcggatc gctgctcggc gggatgctca ccctgatcgg caccccgccc
    11701 aacatcatca tcgccgctta ccgggaacac gcgaccggga cggccttcgg catgttcgat
    11761 ttcctgccgg tgggcgccgc cgtcaccctt gccggggtcg cgttcatcac cctgctgggc
    11821 tggcgcatga cgccacggcg gacggacgac ggcggcggcg gagacctttt cgatatcggc
    11881 ggctacctgg ccgaagtcct ggttcctccg gacagcactt tcgtcggcct tacgctgcgt
    11941 cacgccctcg cggaattggg ggacgacgcc gaggtcgtca tcatcgggct ggtccgcaag
    12001 ggtgagatgc aggaaatgcc gtccgtgttc gagatcctgc acggcgggga cattctcgtg
    12061 atcgaggccg gttccgagga cctggacacc ttcatacacc gcacgggatt cgagatcgag
    12121 gccggaaccg cgcaggatgg atcccccgat cctgaactat cgctccacct ggccgaaatc
    12181 atcgtcacgc catcttcctc gctcgtcgga cggaccgccg tccgggtcga ccttcgaaac
    12241 cgctacggcc tgaacctcgt cgccgtcgcc cgacaggggc accgcctgaa cgagcggctc
    12301 gggaacgtcc ggttcgtcgc cggggacatc ctgctggtgc agggcgaggc atcgaccctc
    12361 aagtccaggc tcgccgagct cggctgcctg ccgctggcct cccgcggcct gcgcataggc
    12421 aagccgcgca acgtgatcct gtccgctgcg atcttcggag ccgtgctcgc cgccgtctcc
    12481 ctggacgtcg ttccgcccgc cacggccatg atcgcggggg ccgtcgcgat ggtgctcgtc
    12541 ggactcatcg ggaaggatga atcgtatcgc agcatcgaca tgcccgtgat cgtcctcctc
    12601 gccgccatgc tgcccatcgg cgaggccctg gaaacgacag gcggatcccg tctcatagcc
    12661 gacgcgctgc tcgcgggatc ggcgtccctt ccgcccgcga tgatgctcgc catgctcatg
    12721 acggcgatca tgctcctctc caacgtcatc aacaacgcgg cggccgccgt cctctccgcc
    12781 ccgatcgcga tcgacctggc gcacggactg ggagcctcgg tggacccgtt cctgatggcg
    12841 gtcgccgttg cgtcgtcttc ggccttcctg acgccgatcg gccaccagtc caatgccctg
    12901 gtcatggccc ccggggggta ccgtttcggg gactactggc ggatggggct gccgctatcc
    12961 atcctgatcg tggccatcgc cgttcccatg atcctgtggg tatggccgct ctgaacgact
    13021 tccggcagtt ctacccgagg aggctggcca tgcatcgact cgctccgggc catgccaggc
    13081 ttccgcgatt gggccgggac aacggatgtc ccgtggaccg acgtgacccg ggtatcgtac
    13141 tcgcgccggg accgggcagc cggtctgggc gatggcctgc tctccggcgc cgtgggaacc
    13201 ggcccggtcc tcgccggcgt gctcctcgga agccggaggg tcaccggctg ggagcgggcg
    13261 cccgctcccc gttgacgtcc ggactacccc ctcggatggt atccctaatc gttgcgacgt
    13321 gacgacctgg gtgcgccccg cctaccgcac gaacccgccg aacgctccga ggtgcgccgc
    13381 gccgaagccc gcgaccaccg cacgcacttc cgcgctcggc gagccgtaca cgtcgaaccc
    13441 cgtcggctgg acgcatccga gaaagcgctc ggcgaaacgc gagccgaacg cggcgaggtg
    13501 gaccatcacg gcatccgagt cggtgaaccg ctcctggatg tgacaggccg atccgtcctc
    13561 gctcagaaac cactcgtaac ccagagcgcc gggttcctgc cgcgtggact cgaccatctc
    13621 gttcatgagc gcccggaaat tctccagttg tccctcgcgc acggcgaggc gcagattcca
    13681 ggacacgacg ttcgacatgc gagtacctcc ggattctctt ggtcggggat ttcggctgtt
    13741 gccgggcgtt gcgcgcagtt gcgggcagtt gccggctgtt gccgacacat tttagggcac
    13801 ctgtgcgggt ggggcaatgt gcggccggcg cagcgcttcc ggcaccgggg gatttgcccg
    13861 ccgtcatgta gcttacggct gcatcgacgg tatccttccc gcatgaagca catcaacctc
    13921 gaccggctgc cgaagggtga gctgatccgg cgcgccgatc tccacacagc gtacggagga
    13981 tcgaagaagg ccgacgtggc cccgagccgg aaggccggcg gactcttcct cttctcggcg
    14041 gacgccggcg ccccctacga catccgcgac ggctggagcg cgccgaacga gtacgagctc
    14101 tgcgtggtgg tggacgatcc cggacacagc tgggacacgg cttcgcggtc catccgggac
    14161 caccagaagg ccggccggcg gatgtacctc ttccggacga ccagccccga aatgtgccgg
    14221 tacctggggc gggtcgagct gcgcggctac cggaccgagc aagtgacaga tcgcgcccga
    14281 cggacgcgca cggccgtcat cttcacgctc cgggagtccc ggctggtgct cgacatgtcg
    14341 gcgtcggacc actcgctggt cgactccgcg atgaagcagg tcgccgagcg cctgcgccag
    14401 gagggctcgg attttgtcgg ccccgtcccc ctgccctccg accgtgacgc gtccgagaac
    14461 ggggacggcg cgcccgggcg catgcaccgc cgcagcatct acgtccggtt cccggaggcc
    14521 tcgaccatcg cggcgcttac ggaactcccg cttccgtccg gaatcgagat cgagttgagg
    14581 caggagtagg tgggtttgag gggcgcgggg ggcctcgtag aagagcgtag gtgattcctc
    14641 atcccgggcg ggctttcggg cgatcggcga taccggcgtc gcccggcccg aatcctcgcg
    14701 ctccgccgcc gaccccgtgc acatgtcccg ccgatgcttc atctttggcc ctgcgtgcgg
    14761 cgcctcacca gccacgcgga cccatgccct tcggaccctt cggcttcgtc atcgcgatca
    14821 tcatggtcgt gttcggcttc gtcgccttta ccgtcaagct gaacaacgac tacaagctcc
    14881 ggcgcgaaga gctgcagagc ggcagggagg gctcgagcct cggctcgggc gagttgaggc
    14941 agctcatccg cgaggccgtg gccgatgcaa tcgtgccggt cgaggagcgc ctcgaactca
    15001 tcgagcggca gatgaggcag ctccccgagc cccgtccggc gccccctcgg cagcccggac
    15061 cgggtgacga acccacgagg gggcccctgc catgacgcta cccctctccc gacgccggtt
    15121 cctcaggacg accggcgcgg caggcctcgg cgccgccctc gtgggcctgc cgcggtgcgg
    15181 ccgggccgtc ggtgccggca ccgtgagggt gctctcgatc ggcgtcgtgg ggacgatcgg
    15241 cgggtgggac cgcgagcaga tcgcggacca tccgcgggcc gagatcgtgg gcctgtgcga
    15301 cgtggatgcc gcctacctgg cccaggccgc cgccgaccat ccggacgcct tcacgtgccg
    15361 ggactaccgc gaggcgttcg cgcgctacgc ggatcgcttc gacgcggtga tcgtcgcgac
    15421 ccccgaccac acgcacgcgc cgatcatgct gacggcgctc gcgcacgaca agcacgtcta
    15481 cggccagaag ccgctggtgc accagctcga ggaaatcacg ttgatcgagc gggcgatgcg
    15541 ggccaagccg ggcctcgtga cgcaggtcgg caaccagcgc atggtgaacc cgggacgtcg
    15601 cgcggccgtc gagatcctgc gctcgggcgc gctcggacgg gccgtcgagg cgcacatctg
    15661 gacgggcggc ccggacgact cgatctactt caattacggc cgggtcctga gcgatccgct
    15721 tccgccgccg cccggcctgg actgggacct gtggctcggc ccctgcgagc cgcggccgta
    15781 ccgggacctc ctcgtgccgg ccaagtggcg gtcgtggtgg gacttcggca ccggcggcat
    15841 cggcgactgg ggcgtgcacc tgctcgacgt catcttcttc gccttcgacg agctgacgac
    15901 cccggtctcc gtcgtcgcgc acaccccgga gcccgccggc acgttccaca ccttcccgac
    15961 ccggacgacg acgacctacg cggtcgattc ggaccgtttc gccgggagca tcttccccat
    16021 ccactacaac gacgtcgccc aggcgccgcc gcgcgaatcg ctcgggctgc cggccggcgg
    16081 ttggatgggc accaacatga cggtcgtcgt ctgcgagggc ggcgtgatgg tgctcgcgcc
    16141 ggagggcgag ctcgagatct ggcgcgacgg ccgcatgaca cccgggctcg agatgccggg
    16201 cctgcccgaa ttcccggagc tcaaccactg gcatgcgtgg gtcgacacga tcctcggcgt
    16261 cgagaccgag cttcggacgc ccttcgtcga cggcgcgcgg atcacggagg ccgctcttct
    16321 ggccgtgaag gcgacccgct tccccggcca ggagctcctg tgggacaagg cgtcgctctc
    16381 gttcaccaat caccctgagg cgacggccgc catcgtgcgc cgggactacc gggaggggtt
    16441 cgcgccgccc gtggtggggt gacggtacgg ccttgcggct cgggtgactt cggagggtca
    16501 ggtaggtgtc tgtatgcact ggacaccctc tggaagccgt gtccgattgt ttccaccggt
    16561 agcaatcggt cctttcttcc ggtatgtccc ggcaggaccg catcacgcac ccgccacctt
    16621 tccccaggag cccccaggta aggaaccacc cgagggggag caccggatac gaccctaccg
    16681 cgccacgatc cacgtccggc tcgcatggaa gccggggccg cgcagccgcg ccaggtacac
    16741 gccgctcgag aggcccgacg cgtcgaactc ggcctcgtgc gtgcctgtct gcaaaacctc
    16801 gtcgaccacc gtgcccaccc gacggccgag cacgtcgtac agctccacga ccacgcggcc
    16861 gggcgctccc accgagtacg cgaaccgggt ccgtccaccg aaaggattcg gccagttcgg
    16921 gcccagcgac cagccatcga ggggcaggtc gcgctcgacc gcgacatccg aggccgctcc
    16981 gaagtccacg cggatggcgt cggcgcgaag gacgcccgtc gagccctcgg cttcgctcac
    17041 ctcgaccgcg atctcgtcgc ccgtcgagaa gagccaggcg ccgagcgccc gccaggcacc
    17101 gcttccctgg ttctggtcga tgaccagcga ctcgacgagc tcgccgttcc gaagcaccct
    17161 gtactgggcc tgcgtcgacg cgttcaccgt ggtcggtacg atttcgtgca gggcgtgcac
    17221 tccggtgacc tccgccacgg agcggaacgt cgccgtcgcc ccgctgccct gcccggcgta
    17281 gcggctgctc gtgccgtagg cctgggaatt gctcgacagc cacgtgccgg cttcctcgta
    17341 gtgctccgcg tcggcgtcgt cgaggaccgc cgtccggagc tcgtcgccca tgcgggaaag
    17401 gcggaacgcg tcggcccgca gcacccggcc cgactggtcc gggtccgacg cccgcacggt
    17461 cacgcggacc acggagcccg cctcgagctc gtagaggccc agcgggcgcc acgccccgct
    17521 gccctggttc tggtcgaccc gtacggagtc gatgcgcacg ccgtcgaccg acaccaggta
    17581 gtcggcccgg acggcggcat tcacggtgga cggcacgaag taggaggcgt cgaaaacccc
    17641 gccctgctgg accgtcgtcg tgaagctcgc cgtcgcggtc cacgcggacg gaagacccgc
    17701 gtaccggctg ctcggaccgt aggcctgcgt gacgctcgtg ttccacgcgc ccgattcctc
    17761 gtaggacgcc gggtcgtcgt tgtccgcgac cgcgaaccag tcgcggaacg tgacctggaa
    17821 ggggacgccc cggaccggct cgatcggatc gtcgctgtac acgaagaggg tgtcccggac
    17881 cacgccgatc gtc
//
LOCUS       DBNHQZ010000026         5541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1496497, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000026 DBNHQZ010000000
VERSION     DBNHQZ010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1496497"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC16YU_00660"
     CDS             complement(<1..387)
                     /locus_tag="AC16YU_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063760.1"
                     /translation="MSGALVAALAALAAAPLRAPCGGTARASPAPADTVLEVHRIASF
                     RSAASVSVDPSGELVVSDAGRDAVYRLAPDGRVLEALGGTGADAALFDGPHGVDATNG
                     LILLVADAGNGRVQRFARDGRFLERLP"
     gene            complement(384..1676)
                     /gene="purD"
                     /locus_tag="AC16YU_00665"
     CDS             complement(384..1676)
                     /gene="purD"
                     /locus_tag="AC16YU_00665"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6063761.1"
                     /translation="MQILVVGGGAREHAIARCLVGSPSNPRVLVAPGNPGTASIAENV
                     PVRADDIDGQVVLVGERRIDLVVVGPEVPLVMGLADRLEEAGVPVVGPSAAAARLEGS
                     KAFAKEFMARHDIPTASYRTFTADQYAEARAFVEEEGAPIVVKASGLAAGKGAMVCGS
                     VAEALGALTTIMRDGAFGGAGDFVVVESFMHGEEASLFAISDGSDYVMLASAQDHKRI
                     GEDDTGPNTGGMGAYAPAPIVTPQIEEMARTRIIEPTLAGMRAEGTPYRGVLYVGLMI
                     DEDGPRVVEYNCRLGDPEAQVVLPLFEGDAVELFERAATGRLAGYEVPGSRGAAACVV
                     MASPGYPGPYEKGKAISGIEKAEALEGIAVFHAGTSGSARSGYVTSGGRVLTVSAVAD
                     SLGDALDRAYRGVAQITFEGAQFRRDIGRKGLERLRSA"
     gene            complement(1751..2296)
                     /locus_tag="AC16YU_00670"
     CDS             complement(1751..2296)
                     /locus_tag="AC16YU_00670"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6063762.1"
                     /translation="MLHPFLDVVPRLGDSVFVAESAQVIGDVHLGAESSVWFNAVVRG
                     DVNWIRIGAASNIQDNAVVHVTNRTAPTLIGDRVTVGHSAVVHGCTVEDDVLVGIGAV
                     ILDHAVIGHDSLVGARALVTGGTRIPPRSLVLGSPARVVRPLSDEEVEQVHAFARNYV
                     GYARIYRGVDRPARNPFYDPT"
     gene            complement(2324..4999)
                     /gene="mutS"
                     /locus_tag="AC16YU_00675"
     CDS             complement(2324..4999)
                     /gene="mutS"
                     /locus_tag="AC16YU_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6063763.1"
                     /translation="MAARKQAGTGSVTPLMRQYYRIKERHADAVLLFRMGDFYETFED
                     DAKKVHEVLGITLTKRSNGQAADVPLAGFPHHALDTYLPRLVSAGLRVAICEQLEDPK
                     NAKTVVKRDVVEIVTPGVSLHDRLLDPRRSNYVAAVHVDAAGTRAGVAYLDASTGEFA
                     VTEVAPSRVEGVLQTVQPAEIIRMKGVDPPKGAADAVLTRLEDWIFTREYAEETLLRH
                     FRTHSLKGFGLEDLSVGIVAAGAVLHYVSETQQGRIPHVRSLTRLAPDGYMALDPQTK
                     RNLELVASMQGGSAEGSLVSILDRALTPMGARMLRRRLIRPLTDVGEIHRRLDAVDAL
                     YASRRLREALRSMLGPVGDVERVAARAATGRAGPRDLDQLRTTLGLVPGIRELLAEED
                     CAPLRELGDLLDPCTDLAEELRAGLVDEPPAAVRDGGVIREGFSAELDEIRTLARSGK
                     DFIARLQQEEVRRTGIPSLKVGYNKVFGYYLEITNAHRDRIPDDYIRKQTLVNAERYV
                     TPALKEYEEKILTAEERIASLEAELFAGLRERVARALPVLQQNAHCLAELDTHSALAE
                     VAVRNRYVRPYVHDGRDIEIENGRHPVVERALPPGEPFIPNSVRLDPESEQILLITGP
                     NMAGKSVALRQVGLIVLLAQVGSFVPAEKARIGVVDRIFTRVGASDNLAAGESTFLVE
                     MNETASILHNATPRSLILLDEVGRGTSTFDGLSIAWALVEYLHERPDVAARTLFATHY
                     HELNELADRYPRIRNFRVQVQEHGDKVVFLRRLVPGGADHSYGIEVARMAGLPDELIG
                     RAREVLRHLESQQLAEAVGESGNDTGGSARSPGLPAAVPAGTFQMSLFGAAEPDPLTE
                     AVREKLRNVDPDRMTPIEALLLVSSLRRLAEEHGD"
     gene            complement(5130..>5541)
                     /locus_tag="AC16YU_00680"
     CDS             complement(5130..>5541)
                     /locus_tag="AC16YU_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140664 - ATP-dependent DNA damage sensor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA family protein"
                     /protein_id="HIR6063764.1"
                     /translation="GGRALKFYASVRMDIRRIGAIKDGTEVVGNRTRVKVVKNKVAPP
                     FREAEFDIVYGEGISSLGELIDLGVERDILQKSGSWYAYGDMKVGQGRDAAKQWLREN
                     DALRAEIREQLREALGMNPPPVGVTNGAVVEEEG"
BASE COUNT          810 a         2012 c         1885 g          834 t
ORIGIN      
        1 cggaagccgc tcgagaaacc tcccgtcccg cgcgaaacgc tgcacgcgtc cgtttccggc
       61 atccgcgacc agcaggatca ggccgttcgt ggcatcgacg ccgtgcggcc cgtcgaacag
      121 ggccgcgtcg gcgcccgttc caccaagtgc ctccagcacc cggccgtccg gcgccagccg
      181 gtagacggca tcgcgcccgg cgtccgatac gaccagctcg ccggacgggt cgaccgacac
      241 cgacgcagcg ctccggaagg acgcgatcct gtgcacctcg agcacggtgt ccgccggagc
      301 cggggatgca cgagccgttc cgccgcacgg agcgcggagc ggagccgctg cgagggcggc
      361 gagggcggcg accagcgcgc cgctcatgcc gaacggagcc gctccagtcc cttgcggccg
      421 atgtcccgcc ggaactgcgc cccttcgaac gtgatctgcg cgactccgcg gtacgcgcgg
      481 tcgagcgcgt cgccgagcga atccgccacg gcgctcaccg tgagcacgcg ccccccggac
      541 gtcacgtacc cggaccgagc acttccggag gtacctgcat ggaacaccgc aatgccttcc
      601 agcgcttcgg ctttctcgat gccggatatg gccttcccct tctcgtacgg gcccgggtat
      661 ccgggcgagg ccatgaccac gcaggccgcg gcgccacggg atccggggac ctcgtacccg
      721 gcaagccgcc cggttgccgc gcgctcgaac agctccacgg cgtcgccctc gaatagcggc
      781 agcaccacct gggcctcggg gtcgccgagg cggcagttgt actccacgac ccgcggaccg
      841 tcctcgtcga tcatgagccc cacgtagagg acgccccggt agggggttcc ctccgcgcgc
      901 atgccggcca acgtgggctc gatgatccgt gtccgggcca tttcctcgat ctgcggcgtc
      961 acgatcggag ccggggcgta ggcccccatc ccgcccgtgt tcgggccggt gtcgtcctcc
     1021 ccgatgcgct tgtggtcctg ggccgaggcc agcatcacgt agtcggatcc gtccgagatc
     1081 gcgaacaggc tggcctcctc tccgtgcatg aaggactcga cgaccacgaa atcgcctgcc
     1141 cctccgaacg ccccgtcccg catgatcgtc gtcagcgctc cgagggcttc cgccacgctc
     1201 ccgcacacca tggcgccctt ccccgccgcg aggccgctcg ccttgacgac gatgggcgca
     1261 ccctcctcct cgacaaacgc acgggcctcg gcgtactggt cggccgtaaa cgttcggtag
     1321 gacgccgtcg ggatgtcgtg gcgggccatg aactccttgg cgaaggcctt gctgccctcg
     1381 agccgggctg ccgcggccga cggcccgacc accggaacgc ccgcctcctc cagccggtcg
     1441 gcgagcccca tgaccagggg cacctccggt cccacgacga ccaggtcgat cctccgctcg
     1501 ccgacgagaa ccacctggcc gtcgatatcg tcggcacgga caggcacgtt ctctgcgatc
     1561 gaggccgtac cggggttgcc gggcgccacg agcacgcgcg ggttcgaggg acttccgacc
     1621 aggcaacggg cgatggcgtg ctcacgcgcc ccgccgccga cgaccagtat ctgcatgcga
     1681 gcttcggatg aaccggatcc gcgggcagta tacgcgatga tccggcgaaa tacggcgcga
     1741 agccggccgt tcaggtcggg tcgtagaagg gattgcgcgc cggacggtcc acgccgcggt
     1801 agatccgggc gtagcccacg tagttcctcg cgaaggcgtg cacctgctcc acctcctcgt
     1861 ccgacagcgg acggacgacg cgggccggac ttcccaggac cagggaacgc ggcgggatcc
     1921 gggtcccccc cgtcacgagg gccctcgccc cgacgagcga gtcgtggccg atcaccgcgt
     1981 gatcgaggat caccgcgccg atccctacga gcacgtcgtc ctcgaccgtg cagccgtgca
     2041 cgacggccga gtgccccacg gtgacccgat cgccgatcag ggtcggtgcc gtccggttcg
     2101 tcacgtgcac cacggcgttg tcctggatgt tcgaggccgc accgatgcgg atccagttga
     2161 cgtcgccccg cacgaccgcg ttgaaccaca cgctagattc ggcgccgaga tgcacgtcgc
     2221 cgatgacctg ggccgactcg gctacgaaga ccgagtcgcc gagccggggg accacgtcga
     2281 gaaaaggatg cagcatggat gcagtgaggt gtgcgctcgt taatcagtcg ccgtgctctt
     2341 cggccagtcg cctgagcgac gaaaccagga gcagggcctc gatgggcgtc atccggtcgg
     2401 gatccacgtt ccgcagcttc tcccggacgg cctcggtgag cgggtcgggc tcggccgcgc
     2461 cgaagagcga catctggaac gtgcccgcgg ggacggccgc ggggagtcca gggctgcgtg
     2521 ccgagccccc ggtatcgttt ccggattcgc cgacggcttc ggccagctgc tgggactcga
     2581 ggtggcgcag gacctctcgg gcgcgcccta tcagctcgtc cggcagcccc gccatccgcg
     2641 cgacctcgat gccgtacgag tggtccgccc cgcccggcac caggcgcctc aggaagacca
     2701 ccttgtcccc gtgctcctgc acctgcaccc ggaaattgcg gatccggggg tagcggtcgg
     2761 cgagctcgtt cagctcgtgg tagtgggtcg cgaagagcgt cctcgccgcg acgtccgggc
     2821 gctcgtgcag gtactcaacc agcgcccagg cgatcgacag cccgtcgaag gtggacgtgc
     2881 cgcggcccac ctcgtccagc aggatgagcg aacggggcgt cgcattgtgg aggatgctcg
     2941 ccgtctcgtt catctcgacg aggaacgtcg actccccggc ggcaaggttg tcggaggccc
     3001 cgacgcgcgt gaagatgcgg tccaccacgc cgatgcgcgc cttctcggcg ggcacgaacg
     3061 agccgacctg ggccagcagc acgatgagcc ccacctggcg gagggccacg ctcttgcccg
     3121 ccatgttcgg gcccgttatg agcaggatct gctcggactc cgggtccagc cggacggagt
     3181 tcgggatgaa aggctcgccg ggcgggagcg cgcgctccac gacggggtgg cggccgttct
     3241 cgatctcgat gtcgcggccg tcgtgcacgt acggccggac gtaccggttg cgcacggcca
     3301 cctcggcgag cgccgagtgc gtatcgagct cggccaggca atgggcgttc tgctggagta
     3361 cgggaagggc ccgcgccacg cgctcgcgca ggcccgcgaa gagctccgcc tcgagagaag
     3421 cgatccgctc ctccgcggtc aggatcttct cctcgtactc cttgagcgcg ggcgtcacgt
     3481 accgctcggc gttgacgagg gtctgcttgc ggatgtagtc gtcggggatc ctgtcccggt
     3541 gcgcgttcgt gatctccagg tagtagccga agaccttgtt gtagcccacc ttgagcgacg
     3601 ggatgcccgt gcgccggacc tcctcctgct gcagacgggc gatgaagtcc ttgccggagc
     3661 gggcgagcgt ccggatctcg tcgagttcgg ccgagaagcc ctcgcggatc acgcccccgt
     3721 cccgaacggc ggcgggcggc tcgtccacga ggccggcccg gagctcttcc gcgagatcgg
     3781 tgcaggggtc caggaggtcg cccagctccc ggagaggggc gcagtcctcc tccgccagga
     3841 gctcccggat gccgggaacc aggccgagcg tggtccggag ctggtccagg tcccggggcc
     3901 ccgcgcggcc ggtggcggcc cgtgcggcca cgcgctccac gtcgccgacg ggcccgagca
     3961 tcgagcggag ggcctcgcgc agtcgccgcg acgcgtagag cgcgtccacg gcgtcgagcc
     4021 gccggtggat ctcgccgacg tcggtgagcg gacggatcag ccggcgtcgc agcatccggg
     4081 cgcccatcgg cgtgagcgcc cggtccagta tcgagacgag cgagccctcc gcgcttccgc
     4141 cctgcatcga ggccacgagc tccaggttgc gcttcgtctg cggatccagc gccatgtacc
     4201 cgtccggcgc caggcgagtg aggctcctga cgtgcggaat gcggccctgc tgcgtctccg
     4261 acacgtagtg cagcaccgcg ccggccgcga caatgcccac cgagaggtcc tcgaggccga
     4321 atcccttcag cgaatgggtc cggaagtgcc tcagcagcgt ctcctcggcg tattcccggg
     4381 tgaagatcca gtcctcgaga cgcgtgagga cggcgtcggc ggcgccctta ggcggatcga
     4441 cgcccttcat gcggatgatc tccgccggct gcacggtctg cagcacgccc tccacacgcg
     4501 acggcgcgac ctcggtcacc gcgaactcgc cggtcgacgc gtccaggtag gcgacgcccg
     4561 cccgcgtgcc ggccgcgtcc acgtgcaccg cggcgacgta gttggagcgc ctggggtcga
     4621 gcaggcggtc gtgcagggaa acgcccggcg tgacgatttc cacgacgtcc cgcttgacga
     4681 cggtcttggc gttcttcggg tcctcgagct gctcgcagat cgccacgcgc aaccccgcgc
     4741 tcaccagccg gggcaggtac gtgtccagcg cgtggtgggg gaacccggcg agcggtacgt
     4801 cggccgcctg cccgttcgac cgcttcgtga gcgtgatgcc gagcacctcg tggaccttct
     4861 tcgcgtcgtc ctcgaacgtc tcgtagaagt cgcccatccg gaacagcagt accgcgtcgg
     4921 catgccgctc cttgatgcgg tagtactgcc gcatcagggg agtcacgctg cccgtgccgg
     4981 cctgcttcct ggccgccatg atcctcgccg cctgtgatgg gttcggtgcc ggaccgacgg
     5041 gcgccgggcg atcggcgcgg aaatgcggac gcgcccggtc cggcagtccc gccgaaggta
     5101 atgaagccgg cgggcaacga cccggtggat cagccctcct cctccaccac ggcgccgttg
     5161 gtcacgccca cgggcggcgg gttcatgccc agggcctcgc ggagctgctc cctgatctcg
     5221 gcccggagcg catcgttctc gcggagccac tgcttggccg catcccggcc ctgtcccacc
     5281 ttcatgtccc cgtaggcgta ccaggagccg ctcttctgga ggatgtcgcg ttcgacgccg
     5341 agatcgatca gctcgcccag cgacgagatg ccctccccgt acacgatgtc gaactcggcc
     5401 tcgcggaacg ggggcgcgac cttgttcttg acgaccttga cccgggtgcg gttgccgacg
     5461 acctccgtgc cgtccttgat ggccccgatc cgccggatgt ccatgcgcac cgatgcgtag
     5521 aacttgagcg cgcgcccgcc g
//
LOCUS       DBNHQZ010000027         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3664374, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000027 DBNHQZ010000000
VERSION     DBNHQZ010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3664374"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /locus_tag="AC16YU_00685"
     CDS             <1..2300
                     /locus_tag="AC16YU_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6063765.1"
                     /translation="EKGPMKLQDAVNIAGQVAEALKEAHAKQIVHRDIKSANVMLTTQ
                     GVAKVLDFGLAKTNQSTMLTRMGSTLGTVAYMSPEQARGHEVDSRSDLYSLGTMLYEM
                     VAGRLPFGGDYEQAVLYGIMNEPPEPLTAVRTGVPMELERIVAKLLSKEAEYRYQTAA
                     DLLADLKSLSLGGSGVTRRSMPSMSASQVPVQPSTSLPKWVWGAITGALVLGAVIAWV
                     AKPIPERSVDPVRKVVIPLDGITDYRSPTLSSDGRFLAFGGADTTENIPLLRIQDMET
                     GERMTVPNSNFPNDLRFSPDDSQLCFNSDGRLKVVSASGGDPVTLSGPGSDGCVWDPD
                     GIVYHSTDDGSLEEIRRDGSRRIILEADSASGYYPTEWLPGGFLIAVSGGAGNSHALA
                     VNVADGSTIEILPKPEAWHARLLGNEFLLAQVSPQAQLLAYPFDIDAMRVTDRPVSVV
                     DNITWYDWSVSKSGDFVSVPQSQITGKRLIRWVEGRRELATEQSMDFEDLQASPDGRM
                     VAVEVNAYRGGDDQILVLDLATEAFRQLTVEGHNSEPTWSPDGTQIAYARGGGTFNRL
                     VVQRSDGSGSEIVYPDRGLRTLQNPDFSPDGESIALFARMVDGSSTDFDIHRVNLRDS
                     SVVTLRADAGSQEHPRYSPDGRFIAYDSDETGQTEVFVMTADGSSKWPVTQSGGRYPV
                     WSEDGRQLFFRRGNSLFAIQVETDRVFRPIGNPRSVESIGSVFYFDVLDDGFVMVAGD
                     PVPGKADLVTNWIEAVRRRFGRDNP"
     gene            2297..>3016
                     /locus_tag="AC16YU_00690"
     CDS             2297..>3016
                     /locus_tag="AC16YU_00690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6063766.1"
                     /translation="MSNVHASGIAIAGLLLLAPAVSRAQDRTSGEVVAIEAPTRPLPP
                     EAESAGVDRFSFIVYGDTRGRRDGVELQYEHSQVVESMLRSIASLADEPDPVRFVLQS
                     GDAVVDGRDPAQWNVSFVGLINRLTREGGVPYFLAPGNHDVTSAAELDAPGRLVGLRN
                     YLSAVSRLVPPDGATRRLEGYPTYAFGYGNTFVLAFDSNIADDPVQFEWASAQLEGLE
                     RDRYVHVVAFFHHPAFSSGPHG"
BASE COUNT          512 a          954 c         1027 g          523 t
ORIGIN      
        1 tcgagaaggg cccgatgaag ctgcaggacg cggtcaatat cgccgggcag gttgcagagg
       61 ccctgaagga agcgcacgcg aaacagatcg tgcaccggga catcaagtcg gcgaacgtga
      121 tgctgacgac gcagggcgtg gccaaggtgc tcgacttcgg gctcgcgaag acgaaccagt
      181 cgacgatgct gacgcgcatg ggctcgacgc tcgggacggt ggcgtacatg agcccggagc
      241 aggctcgcgg gcacgaggtg gacagccgga gcgacctgta ctcgctcggc acgatgctct
      301 acgagatggt ggcgggcagg ctgcccttcg ggggcgacta cgagcaggca gtgctctacg
      361 gcatcatgaa cgagcctcca gagccgctca cggccgtcag gacgggcgtg ccgatggagc
      421 tggagcggat cgtggcgaag ctcctgtcga aggaggcgga gtaccggtac cagacagcgg
      481 cggatctgct cgccgacctc aagagcctga gcctgggagg aagcggggtg accaggcgat
      541 cgatgccgtc gatgagcgcg tcccaggtgc ccgtccagcc gtccacgagc ctcccgaagt
      601 gggtctgggg tgcgatcacc ggcgcgcttg tcctcggagc tgtcatcgcc tgggtcgcga
      661 agccgatccc ggagcgctcc gtagatcctg taaggaaagt cgtcatcccg ctggatggca
      721 ttacggacta tcggtccccg acgctttcgt cagatggccg cttcctggcg ttcggcgggg
      781 ccgacacgac ggaaaacatt ccgttgttaa ggatccagga tatggagacg ggcgagcgca
      841 tgaccgttcc gaattcgaac tttcccaacg atctccgatt ttcgcccgac gattcgcagc
      901 tgtgtttcaa cagtgacggc cggctgaagg tggtctctgc cagcggtggc gacccggtca
      961 cgctttccgg ccccggaagt gacggctgtg tgtgggatcc ggatggcatt gtgtaccatt
     1021 cgacggacga cggatccctc gaggagatac gccgcgacgg gagtcgccgg atcatactcg
     1081 aggcggattc cgccagcggc tactacccga cggaatggct cccaggcgga ttcctgatcg
     1141 ccgtgtcggg cggtgcgggc aactcgcacg ccctggcggt gaacgtggcg gacggatcga
     1201 cgatagagat tctcccgaag ccggaagcgt ggcatgcccg gttgctcggc aacgagtttc
     1261 tccttgccca ggtctcaccg caggcccagc tgctcgcgta cccgttcgac atcgacgcga
     1321 tgcgcgtgac ggaccggccc gtttcggtgg tcgacaatat cacctggtac gactggtccg
     1381 tgtcgaaatc cggtgatttc gtgagtgtgc cccagtcgca gatcaccggg aagcgactga
     1441 tccgatgggt cgaggggcgc cgagagctcg ccacggagca gtcgatggat ttcgaggatc
     1501 tccaggccag tccggacggc cgcatggttg ccgtggaggt caacgcctat cgtggcggcg
     1561 acgaccagat cctcgtactg gacctggcga cggaggcctt ccgccaactc acggtcgagg
     1621 ggcacaactc ggagccgacg tggtcgcccg acgggaccca gatcgcatat gccaggggag
     1681 gcggaacgtt caaccgcctc gtcgtccagc gctcggatgg atcggggtcg gagatcgtct
     1741 atcctgaccg gggcctccgg accctccaga atccggattt cagtccggac ggcgagtcga
     1801 tcgccctatt tgccaggatg gttgacggca gttcgaccga cttcgacatc caccgcgtca
     1861 acctccgcga ctccagcgtc gtgacccttc gcgcggacgc cggctcccag gagcaccccc
     1921 gatattcgcc cgatgggcgc ttcatcgcgt acgactcgga cgagacgggt cagacggagg
     1981 tcttcgtgat gaccgccgac ggctcgtcca agtggccggt gacgcagtcc ggggggcgat
     2041 acccggtgtg gtcggaggat ggacgacagc tcttctttcg tcgcggtaac agtctattcg
     2101 ccatccaggt cgaaacggac cgggtcttcc gcccgatcgg gaatccgaga tcggtggagt
     2161 cgatcggctc cgttttctac ttcgacgtcc tggacgacgg gttcgtcatg gtcgcggggg
     2221 atccggttcc cggcaaggcc gatcttgtca ccaactggat cgaagccgta cgccgccgtt
     2281 tcggccggga caacccatga gcaacgtgca cgcctccggg atcgccatcg caggtctgct
     2341 gcttctcgcc cctgcagtgt cgcgagccca ggaccgcacg tccggggagg tcgtcgccat
     2401 cgaggcgccg acccgtccgc ttccgccgga ggcggagagc gccggggtgg accgcttctc
     2461 cttcatcgtc tacggtgaca cgcggggccg cagggacggg gtggagctgc agtacgagca
     2521 ttcgcaggtc gtggaatcga tgctccggtc catcgcgtcg ctggcggacg agcccgatcc
     2581 ggttcgcttc gtgctccaga gcggcgacgc cgtcgtggac gggagggacc cggcccagtg
     2641 gaacgtgagc ttcgtcgggc tcatcaaccg gctcacgcgc gaaggcggtg tgccctactt
     2701 cctcgcgccc gggaaccacg acgtcacctc ggcggccgag ctggatgcgc ccggccggct
     2761 ggtcggactc aggaattacc tgtccgccgt gtccaggctc gttccgccgg acggcgcgac
     2821 ccggaggctc gagggctatc ccacgtacgc gttcgggtac gggaacacct tcgtcctggc
     2881 gttcgactcc aacatcgccg acgaccctgt ccagttcgaa tgggcgagcg cccagctcga
     2941 gggactggag cgggatcggt acgtccacgt cgtggccttc ttccaccatc cggccttctc
     3001 gtcgggtccc cacggg
//
LOCUS       DBNHQZ010000028         7207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1632973, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000028 DBNHQZ010000000
VERSION     DBNHQZ010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1632973"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="AC16YU_00695"
     CDS             <1..331
                     /locus_tag="AC16YU_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6063767.1"
                     /translation="DALAAQVGGIGIAPGGNINYDSGHAIFEATHGTAPKYAGQDKVN
                     PSSVILSGEMMFRYMGWTEVSDLIVKAMEKTIGQKRVTYDFHRLMEGATLLKCSEFGE
                     ALVENMG"
     gene            complement(393..1532)
                     /locus_tag="AC16YU_00700"
     CDS             complement(393..1532)
                     /locus_tag="AC16YU_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-queuosine alpha-mannosyltransferase
                     domain-containing protein"
                     /protein_id="HIR6063768.1"
                     /translation="MKVLALEPWYGGSHRNFLDHLVRHSRHEFQTVTMAARFWKWRMH
                     GGAVTMARKAAALYAGDERPDVIFATDMVNLPAFLALTRDRFGSVPVVLYMHENQLTY
                     PLPEDRPRDYTYAYINYLSALAADRLVFNSRFHYEEFMEALPTLLRLFPDYTHLHTVQ
                     KIRSKSTVLHLGMDLRGHDQYASAYAAHPWGPGMPPPIVLWNQRWEHDKNPAGFFRLM
                     NRLDDAGLPFRLILAGERFNEQPDEFDRAFERYASRILHYGYAEDFAEYSRLLHRSDL
                     VVSTAKHEFFGISLMEAIYCGCHPLLPNRLSYPELVPESLHRPLLHAPVLYEDDDDLF
                     RILSAILKGDERPLPVSTLKGIPEHLDWRGHVTHYDDLIEETGKA"
     gene            complement(1564..1815)
                     /locus_tag="AC16YU_00705"
     CDS             complement(1564..1815)
                     /locus_tag="AC16YU_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063769.1"
                     /translation="MALRKRKGMWIKNTHALPVVIEMKSRTLQLGSGEEILVTAEEVL
                     DSTLRAHLQVRAVSIVRPSTDEEEEALIRELAGASDAAE"
     gene            1966..2646
                     /locus_tag="AC16YU_00710"
     CDS             1966..2646
                     /locus_tag="AC16YU_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6063770.1"
                     /translation="MRLATLPRDLLDLLLPSMCLACEDRATDPLPLCASCRLGLAAVE
                     PYDAGRALAHLPGVDGLTALWMLDSGGPARALVHGLKYRGLRRIGPVVGQSLGRRLAR
                     EAAAPYDAVIPVPLHRTRLLERGYNQSEWIARGVAGALGIPVLARALVRRSAASSQTR
                     SGRQARADSVRGAFTVPRADDVAGRRIVLVDDVLTTGATAGEAARAMLEAGAGPVHLA
                     TVGLTRPD"
     gene            complement(2649..3275)
                     /locus_tag="AC16YU_00715"
     CDS             complement(2649..3275)
                     /locus_tag="AC16YU_00715"
                     /EC_number="7.5.2.-"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6063771.1"
                     /translation="MNELIAEGVGQRFGQRVLFRRLDLTARGGETLAVTGSNGSGKST
                     LVRILAGVQTPTRGRVVLRHQGADVPDDERPFLVGLVAPYLNVYDGFSPRENLEFIAR
                     ARRLPAAGERIRATLERVALADRSDDYVGTFSSGMKQRMRFAAALLADPPVVLLDEPT
                     SNLDEAGIRTVADIVLSCIEDGKVVVVATNDAAEAAACDRTLCVTDFR"
     gene            3410..3835
                     /locus_tag="AC16YU_00720"
     CDS             3410..3835
                     /locus_tag="AC16YU_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HIR6063772.1"
                     /translation="MRSVFALLVPVLLLAAACGRSTPETPEPAVPVSVRFASPADGDT
                     LDATFTVRMIVEGVDLAPAGTMEAGTGHLHVLIDTAFVDPGVVIPMDEHHRHFGDAAT
                     EAELTLEPGEHTLRLQLADGAHIALEGGEFRHEIRVVVR"
     gene            3876..5441
                     /locus_tag="AC16YU_00725"
     CDS             3876..5441
                     /locus_tag="AC16YU_00725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR6063773.1"
                     /translation="MKRLLPVAALVVASACSPGTPLDAPSAPSLIPLPASMEALPGSF
                     DATGDLDLSAPSSGWAGTASAVLGIPVETGGDDAAITIGIDGSLPPEGYRLKVDADGI
                     RIGGADDAGAFYALQTVRQLMRADGSVPAVRIEDAPRFTYRGLHLDVGRHFFPVDVVK
                     RYLDHMAALKLNTFHWHLTEDQGWRIQILRYPRLTEVGSCRTETVVEKNYDPFVGDGT
                     PHCGFYTQDQVRDVVAYAAERHITVIPEIEMPGHSVAALAAYPELACTDGPFEVSTTW
                     GIHSDILCPKEETFTFLEGVLTEVMDLFPSRYIHIGGDEAPRTRWEESDVAQAVIRRE
                     GLSDEHELQSWFIRRIETFLNANGRSLIGWDEILEGGLAPNATVMSWRGTEGGIAAAR
                     QGHDVIMTPTRHLYIDFYQGPPEGEPIASNWSGHALPIEDVYAFDPVPAELTPDEARH
                     VLGAQANLWTEFIATEDHLEYMLFPRVLALAEMVWTPAERRSWDDFARRLPERIRALQ
                     ADGVNVRVPEFMR"
     gene            complement(5517..6440)
                     /locus_tag="AC16YU_00730"
     CDS             complement(5517..6440)
                     /locus_tag="AC16YU_00730"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008989883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HIR6063774.1"
                     /translation="MNRRSFLSGGALATAAAATGLAAGPFGAAYAEPASGAPFRMHYA
                     PHLGMFSNHAGDDPVDQLHFMADEGFTAFEDNSMAGREVAVQEAMARAMQDRGLRMGV
                     FVAHTIYWTEPTLTTGRDDQREQFLAEIRSSVDVARRVGATWMTVVPGYEDLRRDLGF
                     QTASVVESLKRAAEILEPHGLVMVLEPLNPRDHPGLFLKRIPQAYTICKAVGSPACKI
                     LDDLYHQQVTEGNLIPNLDAAWDEIAYFQIGDNPGRQEPTTGEINYRNVFGHIHAKGF
                     DGILGMEHGVSGDGIEGEKRLIQAYREVDTF"
     gene            complement(6466..>7207)
                     /locus_tag="AC16YU_00735"
     CDS             complement(6466..>7207)
                     /locus_tag="AC16YU_00735"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6063775.1"
                     /translation="LARGLELAGAWWERTGGLFDASLGAMTRLWRRSMRRGSLPDSAS
                     LAAARAASGWRHVLVDGRTVTFLVAGVRLDFGGIGKGMAADEALRVLRSHGITRAVVD
                     AGGDVVAGEAPPDAPGWIVALPEESGGGRVYLENGALATSGDRYRHVEVDGVRYSHIL
                     DPETGTGRVGGRTVSVLARDGAAADALATAVLLAGPLKAGEFVPGVARAVLATGPDGG
                     TRRFGRLPEAVPDRAPASDCGPGGGRLD"
BASE COUNT         1037 a         2580 c         2489 g         1101 t
ORIGIN      
        1 cgacgccctg gctgcccagg tggggggtat tggcatcgct ccgggtggga acatcaacta
       61 cgactcgggc cacgccatct tcgaggctac ccacggcacg gcccccaagt acgccggcca
      121 ggacaaggtg aacccgagtt cggtcatcct ctcgggcgag atgatgttcc gctacatggg
      181 ctggaccgag gtctcggacc tgatcgtcaa ggccatggag aagaccatcg gccagaagcg
      241 ggtcacctac gacttccacc gcctgatgga gggcgcgacg ctgctcaagt gttcggagtt
      301 cggcgaggcg ctcgtcgaga acatgggctg agcggttccc gctgcggttt cgaaggggcg
      361 gctttccgga aggggggccg ccctttcccg atctacgcct tccccgtctc ctcgatcagg
      421 tcgtcgtagt gcgtgacgtg cccccgccag tccaggtgct cggggattcc cttcagcgtc
      481 gagaccggga gcgggcgctc gtcccccttc aggatggccg acaggatccg gaagaggtcg
      541 tcgtcatcct cgtacagcac cggcgcgtgg aggagcggcc ggtgcaggga ctccgggacg
      601 agctccgggt acgacaggcg gttcggcagc agcgggtggc agccgcagta gatcgcctcc
      661 atgagcgaga tgccgaagaa ctcgtgcttc gcggtcgaca ccacgaggtc cgaccggtgc
      721 aggagccgcg agtactcggc gaagtcctcg gcgtacccgt agtgcaggat gcgcgaggcg
      781 tagcgctcga aggcgcgatc gaactcgtcg ggctgctcgt tgaaccgctc gccggccagg
      841 atcagccgga acggcaggcc ggcgtcgtcg agccggttca tcagccggaa gaagccggcc
      901 gggttcttgt cgtgctccca gcgctggttc cagagcacga tgggcggggg catcccgggg
      961 ccccacggat gcgcggcgta ggcggacgcg tactggtcgt gcccgcgaag gtccatcccg
     1021 agatgcagga ccgtgctctt cgaccggatt ttctgcaccg tgtgcaggtg ggtgtagtcc
     1081 ggaaacagcc tcaggagcgt cggcagcgcc tccatgaact cctcgtagtg gaagcgggag
     1141 ttgaagacga ggcggtcggc cgccagggcc gacaggtagt tgatgtaagc gtacgtgtag
     1201 tcgcgcggac gatcctccgg cagcgggtac gtcagctggt tctcgtgcat gtacagcacg
     1261 acgggcacgc tcccgaaccg gtcccgcgtg agcgcgagga aggccgggag attgaccatg
     1321 tccgtggcga agatcacgtc cggccgctcg tcgcccgcgt acagggctgc cgccttccgg
     1381 gccatcgtca ccgcgccccc gtgcatgcgc catttccaga accgggccgc catggtcacc
     1441 gtctggaact cgtgacgact gtggcggacc agatggtcca ggaagttgcg gtgcgagccg
     1501 ccgtaccacg gctcgagggc aagcaccttc atcggtgtcg ggagtggctc gggggtgatg
     1561 gggtcactcg gcggcgtcgg aggcgcccgc gagctcgcgt atcagcgcct cctcctcctc
     1621 gtccgtggat ggccgcacga tcgagaccgc ccggacctgc agatgggccc ggagcgtcga
     1681 gtccagcacc tcctcggccg tcacgaggat ttcctcgccc gatccgagtt gcagcgtgcg
     1741 cgatttcatc tctatgacta ccggtagggc gtgtgtgttc ttgatccaca tgcccttgcg
     1801 cttcctgagt gccatgcgtg ggccgggttg ctgcgttggg gtcgggcggc cggacggccg
     1861 gcccgaagta tacggcattt tcgaagtgct gcacggtgtt gcgccctccc ggacgacagc
     1921 ccgcgcacct tttcaatcaa tatccgcctc acgacccgcc gcccgatgcg ccttgccacg
     1981 ctgccgcgcg acctcctgga cctgctcctc ccatcgatgt gcctggcctg cgaagaccgc
     2041 gccacggacc cgctcccgct ctgcgcctcg tgccgcctcg gtctcgccgc ggtcgagccg
     2101 tacgacgccg ggcgcgcgct cgcgcacctc cctggcgtgg acggcctcac cgcgctctgg
     2161 atgctcgatt ccggcggccc cgcccgcgcc ctcgtgcacg ggctgaagta ccggggcctc
     2221 cgccggatcg gcccggtcgt gggccagtcg ctcgggcggc ggctggcccg ggaggcggcc
     2281 gccccgtacg acgcggtcat ccccgtgccg ctccaccgca cgcgtctcct ggagcggggg
     2341 tacaaccagt ccgagtggat cgcccgcggc gtcgccgggg cgctggggat tccggtcctg
     2401 gcgcgggctc tggttcgacg gagcgccgcg tcgtcgcaga cccgctccgg ccggcaggca
     2461 cgcgcggact ccgtgcgcgg cgctttcacc gtcccgcggg cggacgacgt ggccggccgt
     2521 cggatcgtgc tggtcgacga cgtgctgacg accggggcga ccgccggcga ggcggcccgt
     2581 gcgatgctcg aggccggcgc ggggccggtg cacctcgcca cggtcggtct cacccgcccg
     2641 gactgacgtc agcggaagtc ggtgacgcag agcgtgcggt cgcaggccgc ggcctcggcg
     2701 gcgtcgttcg tggccacgac cacgaccttt ccgtcctcga tgcacgacag cacgatgtcc
     2761 gcgaccgtcc ggatgcccgc ctcgtccagg ttggacgtcg gctcgtcgag gaggacgacg
     2821 gggggatcgg ccaggagggc ggcagcgaag cgcatccgct gcttcatccc ggacgagaag
     2881 gtccccacgt agtcgtccga gcggtcggcc agcgccacgc gttcgagcgt cgcgcggatg
     2941 cgctcgccgg cggccggcag gcggcgtgcc cgggcgatga actccaggtt ctcgcgcggg
     3001 ctgaacccgt catagacgtt caggtacggc gccacgagcc cgaccaggaa cggacgctcg
     3061 tcgtccggga cgtcggcacc ctggtgcctg agcacgacgc ggccccgcgt gggggtctgg
     3121 acgcccgcca ggatgcggac cagcgtggac ttgccggacc cgttcgagcc ggtgaccgcg
     3181 agggtctccc cgccgcgcgc ggtgagatcc agccttcgga acagcacccg ctggccgaag
     3241 cgctgtccca ctccctctgc gatgagctcg ttcattccgt gccgcaatca gcccgccgtt
     3301 ggcggcgcga agataggccc atgggtgcgc cggaccaatg gaatttccgg cggaacggcg
     3361 cccccggcca ctcgttccat tacccgttgc aacaaccttg attcccggca tgcgttccgt
     3421 cttcgcgctc ctcgttcccg ttctccttct cgccgccgcc tgcggccgct cgacccccga
     3481 gacccccgag cccgccgttc cggtctccgt ccggttcgcc tcgcccgccg acggggacac
     3541 gctcgatgcg acgttcacgg tgcgcatgat cgtcgagggc gtggatctcg ccccggccgg
     3601 cacgatggag gcgggcaccg ggcacctgca cgtcctcatc gacacggcct tcgtcgatcc
     3661 gggcgtcgtc atcccgatgg acgagcatca ccggcacttc ggggacgccg cgaccgaggc
     3721 cgaactcacg ctcgagcccg gagagcacac gcttcgcctg cagctagcgg acggcgcgca
     3781 catcgcgctc gagggcgggg aattccggca cgagatccgg gtcgtagtcc gctgacggac
     3841 ctaccttgga agtccgatca acgatccgga ctccagtgaa gcgtctactc ccggtggcgg
     3901 ccctcgtcgt ggcttccgcc tgctcgcccg gcacgcccct cgacgcgccc tccgccccgt
     3961 ccctcatccc gcttcccgcc tcgatggagg cgctgcccgg ctccttcgac gcgaccgggg
     4021 acctggacct gtcggcgccg tcgtccgggt gggcgggcac ggcgagcgcc gtgctgggga
     4081 tccccgtcga aaccggcggc gacgacgccg cgatcacgat cgggatcgac ggctcgctgc
     4141 cgcccgaggg ctaccggctc aaggtggacg ccgacggcat ccggatcggc ggggcggacg
     4201 acgccggtgc cttctacgcc cttcagaccg tgcgccagct gatgcgggcc gacggttcgg
     4261 taccggccgt ccggatcgaa gacgctcccc gctttacgta ccgcggcctg cacctggacg
     4321 tcgggcgcca tttcttcccg gtggacgtgg tcaagcgtta cctggaccac atggcggcgc
     4381 tcaagctgaa cacgttccac tggcacctga ccgaggacca gggctggcgc atccagatcc
     4441 tccggtaccc gcgcctgacg gaagtgggct cgtgccgcac cgagacggtc gtggagaaga
     4501 actacgaccc gttcgtgggc gacggaacgc cgcactgcgg cttctacacg caggaccagg
     4561 tccgtgacgt ggtcgcctac gcggccgagc ggcatatcac ggtaatcccc gagatcgaga
     4621 tgcccgggca ctccgtggcc gcgctcgcgg cgtaccccga gctcgcgtgc actgacggcc
     4681 ccttcgaagt ctcgaccacc tggggcatcc attccgacat cctgtgcccg aaggaggaga
     4741 cattcacctt cctcgagggg gtgctcacgg aggtgatgga tctcttcccg tcccgctaca
     4801 tccacatcgg cggagacgag gcgccccgga cccggtggga ggagtccgac gttgcgcagg
     4861 cggtgatccg ccgcgagggc ctctcggacg aacacgagct ccagagctgg ttcatccgcc
     4921 ggatcgagac cttcctgaac gcgaacggcc gctcgctcat cgggtgggac gagatcctgg
     4981 agggcggcct cgcgccgaac gccaccgtga tgtcgtggcg cgggaccgag ggcgggatcg
     5041 ccgccgcgcg ccagggccac gacgtgatca tgacgccgac gcggcacctg tacatcgact
     5101 tctaccaggg tccccccgaa ggcgagccga tcgcgtccaa ctggtcgggc cacgcgctcc
     5161 ccatcgagga tgtctacgcc ttcgaccccg tgcccgcgga gctgaccccg gacgaggccc
     5221 ggcacgtgct cggcgcgcag gccaacctgt ggacggagtt catcgcgacc gaggaccacc
     5281 tcgagtacat gctcttcccg cgggtcctcg cgctggccga gatggtctgg accccggccg
     5341 agcgccggag ctgggacgat ttcgcgcggc gcctcccgga acggatccgc gcactccagg
     5401 cggacggcgt gaacgtccgc gtgcccgagt tcatgcggtg aggagccccg ccgcggcgtc
     5461 tccgggcgca cgcggtgggg ggcccgccgc ggcaggccgg tcccgcgctc acgggctcag
     5521 aacgtgtcca cctcgcggta ggcctggatc aggcgcttct cgccctcgat cccgtcgccc
     5581 gagacgccgt gttccatgcc caggattccg tcgaatccct tcgcatggat gtgcccgaac
     5641 acattacggt agttgatctc gccggtggtg ggctcctgcc ggcccgggtt gtcgccgatc
     5701 tggaagtagg cgatctcgtc ccaggcggcg tcgagattcg ggatcaggtt gccctcggtc
     5761 acctgctggt ggtacaggtc gtccaggatc ttgcaggcgg ggcttccgac cgccttgcag
     5821 atcgtgtacg cctgggggat gcgcttcagg aagagccctg gatggtctcg ggggttgaga
     5881 ggctccagca ccatgaccag gccgtggggc tcgaggattt ccgccgcgcg cttcaggctc
     5941 tcgaccacgc tcgcggtctg gaatccgagg tccctgcgca ggtcctcgta tccgggcacg
     6001 accgtcatcc acgtggcacc gacccggcgg gccacgtcga ccgagctccg gatctcggcc
     6061 aggaactgct cccgctggtc gtcccggccc gtcgtgaggg tgggctcggt ccagtagatc
     6121 gtgtgcgcca cgaatacccc catgcgaagc ccccggtcct gcatggcgcg ggccatcgct
     6181 tcctgcacgg ccacctcgcg acccgccatc gagttgtcct cgaaggccgt gaaaccctcg
     6241 tcggccatga agtggagctg gtccacgggg tcgtctcccg cgtggttcga gaacatgccc
     6301 aggtgggggg catagtgcat ccggaagggc gccccggacg ccggctcggc atacgccgcc
     6361 ccgaaggggc cggcggcgag gccggtggcg gcggcggccg tggcgagggc gccgccggac
     6421 aggaaggaac gtcggttcat gaggggctcc gataggattc cagcgctaat ctagccgccc
     6481 gccgccgggc ccacaatcgg aggccggcgc ccggtccggc accgcctcgg gaagccggcc
     6541 gaaccggcgc gtcccgccgt cgggtccggt cgcgaggacg gcgcgggcga cacccggaac
     6601 gaattccccg gccttcaggg ggccggcgag gagaacggcc gtcgcgagcg cgtccgccgc
     6661 cgccccgtcg cgtgcgagaa ccgagacggt ccggccgccg acccgccccg tgccggtctc
     6721 cgggtccagg atgtgcgagt aacggacgcc gtccacttcc acgtggcggt accggtcgcc
     6781 cgacgtggcg agcgccccgt tctccaggta cacacggccc ccgcccgatt cctccggcag
     6841 ggccacgatc catccgggcg cgtcgggcgg cgcctccccg gccaccacgt cccctcccgc
     6901 atcgacaaca gcgcgagtga tgccgtggga ccggagcacg cgcagggcct cgtccgcggc
     6961 catccccttg ccgatgccgc cgaaatccag ccggactccc gccaccagga acgtgaccgt
     7021 ccgcccgtcg acgaggacat ggcgccagcc ggaagccgcg cgcgccgcgg cgagcgaggc
     7081 cgaatccgga agcgaccccc gccgcatgct ccgccgccac aggcgggtca tcgcacccag
     7141 cgatgcgtcg aagagcccgc cggtcctctc ccaccaggcg ccggcgagct ccagcccgcg
     7201 cgccagg
//
LOCUS       DBNHQZ010000029         9968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_7160, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000029 DBNHQZ010000000
VERSION     DBNHQZ010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_7160"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="AC16YU_00740"
     CDS             complement(<1..686)
                     /locus_tag="AC16YU_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR6063776.1"
                     /translation="MTADQGAPIWHKGTRAADWVTRFTVGDDAAWDTLLLPYDCVASE
                     AHAWGLVKAGVLTGEEHAGIANVLRALREEHEAGALVVHVEDEDCHTVIERRLVQALG
                     ETGSRVHAGRSRNDQVLAALRLFLRDRLDALGRSASRLVERLCTLAAAGDVLPLPGYT
                     HMQRAMPSTVGLWALGFAELLVDDVERLGEARRRINRSPLGSAAGYGVPYVALPREDV
                     ARRLGFEGTQ"
     gene            complement(676..1761)
                     /locus_tag="AC16YU_00745"
     CDS             complement(676..1761)
                     /locus_tag="AC16YU_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6063777.1"
                     /translation="MTRRTRAAALLGSLVGIPSLSGEEKAVADWLAAYLGRAGLAVER
                     LKDNLWFSLGAGDDTLILNSHLDVVPPSEGHPYPAFEPTVVDDRLYGRGSVDAKGCVT
                     AMSLAAVELVESGWHPPEGGRLVVAFTTYEEMGGAYNGLETLRPHLPTLSAGIVGEPT
                     SLVPVTSQKGLLILRGVAEGRSAHAARAHLGDNAVVRAAADIGRLAALRPGRVHPLLG
                     PVTVTVTTVEGGKARNVVPDRCVFHVDVRSTPSYSHGELVREIQAAVESRIEVHSDRL
                     VPVETAADARIVRAVLRAVPGAEPAGSPTVSDWVFLRDLPVVKFGPGDSSLSHTADEH
                     MPLDSLEAGVDAYRRVIEAYFEADHDG"
     gene            complement(1758..2534)
                     /gene="argB"
                     /locus_tag="AC16YU_00750"
     CDS             complement(1758..2534)
                     /gene="argB"
                     /locus_tag="AC16YU_00750"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6063778.1"
                     /translation="MKRPVVVKIGGGLLETGSRTGFWRGLSVLREHNPVLLIHGGGPQ
                     ATDVARRLGHEPRIVHGRRVTGDTDIEVVQWTMRGQLSTRIVAEAHRLGIRAVGLSGV
                     DAGILQVRRRPPWVVEGETVDFGWVGDVVATDTRFLVRCLADGVLPVVSPLGVDVAGG
                     VYNVNADTVAESLASALNARELLLVTEKGGLRRGPEGDLVAECDRALYESGMADGWIT
                     EGMRVKLKVAFDALDHGVEAVWIVGADDLDRRGTATRVVA"
     gene            complement(2531..3565)
                     /locus_tag="AC16YU_00755"
     CDS             complement(2531..3565)
                     /locus_tag="AC16YU_00755"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HIR6063779.1"
                     /translation="MADLRHLLDWHLLPDAVWESRIEAAIRASSAPPPLEAARGRSVA
                     LLFFNPSLRTRCSMELAAMRLGAHSSTIVPGQGTWGFAWDEGTTMDGAEAEHIREAVG
                     VLSEYYDAIGVRLFASLTDREADASDRRLHQFVRAARVPVVNLESAAWHPCQALADAA
                     TIVERFGGAAAGRRFVLTWAYHPKALPAAVPNSALLAAARLGMHVTVARPDGFALSDD
                     VMGQARTYAATRGGSVEETTDPDAAFDGADIVYAKAWAGRSVYDDPAAEESARAARRD
                     WRVTAERMGRTREAAFMHCLPVRRNVVVDDAVLDGPSAIHLRQAAYRLWAQQAILSWM
                     WDLPDAGGGA"
     gene            complement(3568..4749)
                     /locus_tag="AC16YU_00760"
     CDS             complement(3568..4749)
                     /locus_tag="AC16YU_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6063780.1"
                     /translation="MNTRETIELEDRWQVPTYRKLPIAVIRGEGAWVWDADGDRYLDF
                     YGGHCVALLGHSHPAITEAVAAQAGELLFYSNFVYSPVRARAASALARLAPEGLRHVF
                     FCNSGTEANETALKLARKATGRPVVAAMVGGFHGRTLGSLSVTWGEGYRGPYERVMGP
                     TEFAPFGNLEAFERLLDERDDVAAVILEPIQSMAGVYEAPPEYFQGLREACDRAGCLL
                     VFDEIQTGVGRTGTFSISESLGVRPDLITMAKSLGNGVPVGAVLVGDAIAEGVRPGDQ
                     GTTFGGGMLAMAAVEATLAVLVRDRLMGRAVEIEARVRERAGPLVREIRGRGALLGLD
                     LGRPARPVLDALFERGVIAGNANDPHVVRIMPPLVTGDEEIDEFASILEAVLASVEVA
                     G"
     gene            complement(4754..5785)
                     /gene="argC"
                     /locus_tag="AC16YU_00765"
     CDS             complement(4754..5785)
                     /gene="argC"
                     /locus_tag="AC16YU_00765"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6063781.1"
                     /translation="MNDTSTFRVAVLHGAGYTGAELARLLLAHPTCTLAAVTSRSFAG
                     RPLWEAHPALRGSTDLAFTAPEDFDPSAVDAVFLAAEHGRAAGIVSVLLAGGFDGHVI
                     DLSADFRLRDPAVYEARYGRPHPAPELLPEFVYGLADVRAPYPEGTRLLANPGCFATG
                     LALSLWPLGRGGTFHAAVTALTGASGSGARPSDTTHFPTREGNVRSYRELRHQHLAEV
                     LQLLGPGARVDFVPVSGPWTRGIWGTAHILTSIAPDQVTRAFEEAFADRPLVRLWPDR
                     LPEMRWSVGTPYCDLGWVARDGAVVVGFALDNLVKGASGQAIQNLNLLRGRPETEGLL
                     PSAALQLQA"
     gene            complement(5791..7002)
                     /gene="argG"
                     /locus_tag="AC16YU_00770"
     CDS             complement(5791..7002)
                     /gene="argG"
                     /locus_tag="AC16YU_00770"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6063782.1"
                     /translation="MSIVLAFSGGLDTSFCVPFLREEFGEPVHTITVNTGGVDAAEAR
                     AIEARALELGAASHQTLDGRRLLFDDHLSYLVKGNVLRGSVYPLSVGPERVVQARLLV
                     DAAKRLGARAVAHGSTGAGNDQVRFDVAVRILAGDLEVIAPIREHGLSRAETTAFLKE
                     RGFDVPAKTTTYSINTGLWGTTIGGRETLGTIESVPDDAYPHSTAPADAPDEPLDLVI
                     GWEHGVATVIDGETMDPVAVVERLNRIAGEHGVGRDIHVGDTILGIKGRVAFEAPAAH
                     VLITAHRELEKIVLGKWQRFQKDHLADFYGMLLHEGQHFDPVMRDIEAFLDSSQQVVS
                     GRTRVRLFKGRVSILGCESPFSMFDTGVATYGETNTLWDGRDAQGFARILAVHALIAD
                     RARHGVAEEKE"
     gene            complement(7039..7413)
                     /locus_tag="AC16YU_00775"
     CDS             complement(7039..7413)
                     /locus_tag="AC16YU_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR6063783.1"
                     /translation="MKHLTRADEILLVAVLCLGDDAYSTTIRDEVARRSGKSVTVGSL
                     WVSLDHLADGGYLRKASRRVPGSKGGRPRIYYRLTPKGVRTLKRAREDHARLWEAVPD
                     PDAYRARRPVDRSRDPRRVPRV"
     gene            complement(7486..8916)
                     /locus_tag="AC16YU_00780"
     CDS             complement(7486..8916)
                     /locus_tag="AC16YU_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR6063784.1"
                     /translation="MNRPFDFIEIARQPVRYRPVASRVRDFDEVAVPAPGGVVAEQAR
                     RCMACGIPFCTAGCPLGNLVPEWNALVSADDWHRAWLRLRATNNFPEFTGRICPAPCE
                     SACVAGIPADPVTIEQIELAIVERAWSEGWIVPQPPAVRTGFSVAVVGSGPSGLAAAD
                     QLNRAGHQVTVFERHERPGGLLRFGIPDFKLDKAVLDRRIRLMEEEGIAFRCGVEVRD
                     AASLAGFDAVVLCAGSTRPRDLPVPGRDLAGIHFAWDFLAGQNRAVAGGEQARVPISA
                     AGRHVIVIGGGDTGSDCVGTANRQGAASVTQFELMPMPPPERPSGQPWPFMPAVMKTT
                     SSHEEGAHRLWAVMTAAFEGEARVRTLQTADVAFEGRRAVRIDGTERRWPADLVLLAM
                     GYVGAEAALAEAFGLDLDGGGRIVTTDFATSRRGVFSAGDMRRGQSLVVWAIAEGRDA
                     ARAVDRYLAGSTALPVRGGGDLPRVA"
     gene            complement(8913..>9968)
                     /locus_tag="AC16YU_00785"
     CDS             complement(8913..>9968)
                     /locus_tag="AC16YU_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR6063785.1"
                     /translation="AGAPEHVINYFHFVAEELRGIMASLGVRTLDELTGRTDLLEPAV
                     PEDHPKARTLDLAPILARPGIPEHVASHDRPSGGRDVHSALDHRLVDEARPAIERGTP
                     VTLEARVRNVDRTFGTLLSHAIASRRGLEGLPDGTITIDAAGTAGQSCLAFGSKGITL
                     WLTGGANDYFGKGLSGAIIQVRPPHDAGFRPHEQIIVGNVALYGATSGRVFLRGRAGE
                     RFAVRNSGATAVVEGVGDHGCEYMTGGTVLVLGPTGRNFAAGMSGGTAFVLDGTRDFR
                     SGRCDRSMVDVEPLAEPDDIALVEGLVREHVHRTDSSVGTWVLDHWHEAVAQFVKVMP
                     HEYRRALERMDAEGLAA"
BASE COUNT         1434 a         3699 c         3535 g         1300 t
ORIGIN      
        1 tgctgcgtgc cctcgaatcc gagccggcgc gccacgtcct cccgcggcag cgccacgtac
       61 gggaccccgt agccggcggc gctcccgagc ggcgagcggt tgattcggcg ccgggcctcg
      121 cccaggcgtt ccacgtcgtc caccagcagc tccgcgaagc cgagcgccca gagccccacg
      181 gtcgacggca tggcccgctg catgtgcgtg tatccgggca gcggaagcac gtccccggcg
      241 gccgccaggg tgcagaggcg ctccacgagc cgcgaggccg agcggcccaa cgcgtccagc
      301 cggtcgcgca ggaagagcct cagcgcggcc agcacctggt cgttccggga tcggcccgca
      361 tggaccctgc tcccggtctc gccgagggcc tggaccagac gccgctcgat cacggtgtgg
      421 cagtcctcgt cctcgacatg gacgacgagt gctcccgctt cgtgctcctc ccgtagtgcg
      481 cggagcacat tcgcaattcc ggcgtgctcc tccccggtca ggacgcccgc tttcaccagg
      541 ccccacgcgt gagcctcgct cgcgacgcag tcgtacggga gcagcagcgt gtcccatgcc
      601 gcgtcgtccc cgacggtgaa ccgcgtcacc cagtcggcgg cgcgcgtgcc cttgtgccag
      661 atgggcgccc cctggtcagc cgtcatgatc cgcctcgaaa taggcctcga tgacccgacg
      721 atacgcatcc acgccggctt cgagcgaatc gagcggcatg tgctcgtcgg cggtgtggga
      781 gagcgagctg tcgcccggcc cgaacttcac gaccgggaga tcgcggagga agacccagtc
      841 ggataccgtc ggcgatcccg cgggctcggc acccggaacg gcccgaagca cggcccggac
      901 gatccgcgcg tcggccgccg tctcgaccgg gaccagccgg tccgagtgca cctcgatgcg
      961 gctctcgacc gcggcctgga tctcccgcac gagctcgccg tggctgtagg acggcgtgga
     1021 ccggacgtcc acgtggaaaa cgcaccggtc cggcaccacg ttgcgggcct ttccgccctc
     1081 gaccgtcgtc acggtgacgg tcacgggacc cagcagcggg tgcacgcgcc ccggccggag
     1141 ggcggccagg cgaccgatgt ccgcggcggc gcggaccacg gcattgtcgc ccaggtgcgc
     1201 ccgcgcggcg tgcgcggagc gcccctcggc cacgccccgc aggatgagca ggcccttctg
     1261 agaggtcacg ggcacgagcg aggtcggctc gcccacgatg ccggcggaaa gggtcggcag
     1321 gtgcgggcgg agcgtctcga gcccgttgta cgctccgccc atctcctcgt acgtcgtgaa
     1381 agccacgacc agccgtccgc cctccggcgg gtgccaccct gactcgacga gctccacggc
     1441 ggcgaggctc atcgccgtga cgcagccctt cgcgtccaca gacccgcggc cgtacaggcg
     1501 gtcgtcgacg acggtcggct cgaaagccgg gtacggatgc ccctccgacg gcggcacgac
     1561 atccaggtgc gagttcagga tcagggtgtc gtccccggcg ccgagggaga accacaggtt
     1621 gtctttgaga cgctcgacgg cgagcccggc gcgcccgagg tacgcggcca gccagtcggc
     1681 gacggccttc tcctcgccgc tcagcgacgg aatgccgacg agggacccga gcagcgcggc
     1741 ggcccgcgtc cggcgcgtca tgccactacc ctcgtcgccg tcccgcgccg gtccaggtca
     1801 tccgccccca cgatccacac cgcctcgacc ccgtggtcca gcgcgtcgaa ggccaccttc
     1861 agcttcaccc gcatgccctc ggtgatccag ccgtccgcca tgcccgattc gtagagcgcg
     1921 cggtcgcact cggcgacgag gtcgccttcc gggccgcggc gcaggccccc cttctcggtg
     1981 accaggagca gctccctggc gttcagcgcg gaggccaggc tctcggccac ggtgtccgcg
     2041 ttcacgttgt acacgccgcc cgcgacgtcc acgccgagcg gcgagaccac ggggagcacg
     2101 ccgtccgcaa ggcagcgaac gaggaagcgc gtgtccgtcg ccacgacgtc gcccacccat
     2161 ccgaaatcga ccgtctcgcc ctcgacgacc cacgggggcc ggcggcgcac ctgcaggatg
     2221 cccgcgtcca cgccgctgag ccccaccgcc cggatgccca gccggtgggc ctccgctacg
     2281 atgcgcgtgg agagctggcc gcgcatggtc cactgcacga cctcgatgtc cgtgtccccg
     2341 gtgacgcgac ggccgtgcac gatccggggc tcgtgcccca ggcgacgggc cacgtccgtg
     2401 gcctgcgggc ctcccccgtg aatgaggagc acggggttgt gctcccggag cacgctcagc
     2461 ccgcgccaga atcccgtccg ggaaccggtc tcgagcagtc cgcccccgat cttgacgacg
     2521 acgggccgct tcatgctccg cctccggcgt ccggcaggtc ccacatccag gagagaatgg
     2581 cctgctgcgc ccagagccgg tacgcggcct ggcgcaggtg gatggccgac gggccgtcga
     2641 gcacggcgtc gtccacgacc acattgcggc ggacgggcag gcagtgcatg aacgccgcct
     2701 cgcgcgtgcg ccccatgcgc tcggccgtca cccgccagtc ccgtcgcgcg gcacgcgccg
     2761 attcctcggc agccgggtcg tcgtagaccg atcggccggc ccacgccttg gcgtagacga
     2821 tgtcggcacc gtcgaaggcg gcgtccggat cggtggtctc ctcgaccgac ccgccgcggg
     2881 tcgcggcgta ggtgcgcgcc tgccccatca cgtcgtccga cagggcgaag ccgtcggggc
     2941 gcgcgaccgt cacgtgcatg cccaggcgcg cggcggcgag cagggcggaa ttcggtactg
     3001 cggccggcag ggccttcggg tggtacgccc aggtgaggac gaaccgccgg cccgcggcgg
     3061 ctccgccgaa gcgttcgacg atcgtcgcgg cgtcggccag cgcctggcac gggtgccagg
     3121 ccgccgactc caggttgacg accggcacgc gggcggcccg cacgaactgg tgcaggcggc
     3181 ggtcggacgc gtccgcctcg cggtcggtga gcgaggcgaa gagccgcacg ccgatcgcgt
     3241 cgtagtactc cgacagcacg ccgaccgcct cgcggatgtg ctcggcctcg gcaccgtcca
     3301 tcgtcgtccc ctcgtcccag gcgaatcccc acgtgccctg ccccggcacg atggtcgagg
     3361 aatgggcgcc cagacgcatc gccgcgagct ccatcgagca ccgcgtccgg agcgacgggt
     3421 tgaagaagag gagcgcgacg gaccgtccgc gggccgcctc gaggggcggc ggggcggacg
     3481 aggcacggat ggccgcctcg atcctcgatt cccagacggc gtccgggagc aggtgccagt
     3541 ccagaagatg ccgcaggtcc gccatggtca gccggcgacc tccacggaag cgagcacggc
     3601 ttccaggatg gacgcgaatt cgtcgatctc ctcgtctccg gtgacgagcg gcggcatgat
     3661 gcgcaccacg tggggatcgt tcgcatttcc ggcgatcacg ccccgctcga acagggcatc
     3721 gagcaccggg cgggcgggcc gcccgaggtc gagcccgagc aacgccccgc ggccccggat
     3781 ctcgcggacg agcggtccgg cgcgctcccg gacgcgggcc tcgatctcga cggcccggcc
     3841 catgagccgg tcgcgcacga gcaccgcgag cgtggcctcg acggccgcca tggcgagcat
     3901 gccgcccccg aaggtcgtcc cctggtcgcc cgggcgcacg ccctcggcga tcgcgtcgcc
     3961 caccaggacg gcgcccaccg gaacgccgtt ccccaggctc ttggccatgg tgatgaggtc
     4021 cggccgaacg cccagcgact cggagatgga gaacgttccg gtccggccga cgcccgtctg
     4081 gatctcgtcg aacaccagca ggcagcccgc gcggtcgcag gcctcccgga ggccctggaa
     4141 gtactcgggc ggggcctcgt acacgccggc catgctctgg atgggctcca ggatgaccgc
     4201 ggccacgtcg tcgcgttcgt cgagcagtcg ctcgaacgcc tccaggttgc cgaacggcgc
     4261 gaactcggtc ggtcccatca ccctctcgta cggtccccgg tagccctcgc cccacgtcac
     4321 ggacaggctg ccgagcgttc ggccatggaa gccgcctacc atcgccgcca cgaccgggcg
     4381 gccggtggcc ttgcgcgcga gcttgagagc ggtttcgttg gcctcggtcc ccgagttgca
     4441 gaagaagacg tggcggagcc cctccggcgc cagccgggcc agggcgctcg ccgcgcgggc
     4501 gcgcaccggg ctgtagacga agttcgaata gaagagcagc tcgccggcct gcgcggcgac
     4561 ggcctccgtg atcgcagggt gcgagtggcc cagcagggcg acgcagtgcc cgccgtagaa
     4621 atcaaggtag cggtctccgt cggcatccca cacccaggcg ccctcgccgc ggatgaccgc
     4681 gatcggcagc ttcctgtagg tcgggacctg ccagcggtcc tcgagctcga tggtctcccg
     4741 tgtgttcatg ccctcacgct tgcagctgca gggcggccga cggcaggagg ccctcggtct
     4801 ccggacgccc gcgcagcagg ttcaggttct gtatggcctg gccggacgcg cccttcacga
     4861 ggttgtccag cgcgaatccg accacgacgg cgccgtcccg cgcgacccac ccgagatcgc
     4921 agtacggcgt gccgacggac cagcgcatct cgggcaggcg gtccggccac aggcggacga
     4981 gcgggcggtc cgcgaaggcc tcctcgaagg cccgcgtcac ctggtccggc gcgatggagg
     5041 tcaggatgtg cgccgtgccc cagatgccgc gggtccacgg cccggagacc ggcacgaagt
     5101 ccacccgtgc tcccggcccc aggagctgga gcacctcggc gaggtgctgg tggcgcaatt
     5161 cgcggtagga gcggacgttg ccctcgcgag tcggaaaatg cgtcgtatcg gacgggcggg
     5221 cgcccgagcc gctggctccc gtgagcgcgg tgacggccgc gtggaacgtc ccgccgcggc
     5281 cgagcggcca tagcgagagc gcgaggcccg tcgcgaagca gccggggttc gcgagcaggc
     5341 gcgtgccctc gggatacggg gcgcggacgt ccgcgaggcc gtacacgaac tccggaagca
     5401 gctccggcgc ggggtgcgga cgcccgtagc gagcctcgta cacggccgga tcccggagcc
     5461 ggaaatcggc ggagagatcg atcacgtggc cgtcgaagcc gccggcgaga agcacggaga
     5521 cgatgccggc cgcgcgcccg tgctccgccg ccaggaacac cgcgtcgacc gccgacggat
     5581 cgaagtcctc gggagccgtg aacgcgaggt ccgtcgagcc tcggagcgcg ggatgcgcct
     5641 cccagagcgg gcggcccgca aacgagcggc tcgtgacggc cgcgagcgtg caggtcggat
     5701 gggccagcag gagccgcgcg agctccgccc ccgtgtaacc cgcgccgtgg agcacggcaa
     5761 cgcggaaggt cgaggtgtcg ttcatggccg tcattccttt tcctcggcca cgccgtgccg
     5821 ggcccggtcg gcgatgagcg cgtggaccgc caggatgcgc gcaaatccct gggcgtcgcg
     5881 tccgtcccac agcgtgttgg tctcgccgta ggtcgcgacc ccggtgtcga acatggagaa
     5941 cgggctctcg cagccgagaa tcgacacccg gcccttgaag aggcgcacgc gcgtgcggcc
     6001 ggacacgacc tgctgcgacg agtccaggaa cgcctcgatg tcgcgcatga ccgggtcgaa
     6061 atgctggccc tcgtgcagga gcatgccgta gaagtcggcc aggtggtcct tctggaaccg
     6121 ctgccacttg ccgaggacga tcttctcgag ctcccggtgc gccgtgatga gcacgtgcgc
     6181 ggcgggcgcc tcgaaggcga cgcgtccctt gatccccagg atcgtgtccc cgacgtggat
     6241 gtcgcggccc acgccgtgct cccccgcgat gcggttcagg cgctcgacga ccgcgaccgg
     6301 gtccatggtc tcgccgtcga tcaccgtggc cacgccgtgc tcccacccga tgacgaggtc
     6361 cagcggctcg tccggcgcgt cggccggcgc ggtcgagtgc gggtaggcgt cgtcgggcac
     6421 ggattcgatc gtgccgagcg tctcgcgtcc cccgatcgtc gtcccccaga gacccgtgtt
     6481 gatggaatac gtggtggtct tcgccggcac gtcgaagccg cgctccttca ggaacgcggt
     6541 ggtctcggcg cgcgagaggc cgtgctcgcg gatcggggcg atgacttcca ggtcgcccgc
     6601 caggatgcgg acggccacgt cgaagcggac ctggtcgttc ccggcgcccg tggacccgtg
     6661 tgccacggcg cgcgcaccga ggcgcttcgc cgcgtcgacg agcagccggg cctggaccac
     6721 gcgctccggt cccacggaca gcggatagac cgagccccgc aggacgttgc ccttcacgag
     6781 gtacgacagg tggtcgtcga agagcagccg ccggccgtcg agcgtctggt gggaggcggc
     6841 gccgagctcg agcgcacgcg cctcgatcgc ccgggcctcg gccgcgtcga cgccgccggt
     6901 attcacggtg atcgtgtgca cgggctcgcc gaactcctcg cggaggaacg ggacgcagaa
     6961 ggacgtgtcg agtccgcccg agaaggccag aacgatggac attgcgccgt gggggtcgtc
     7021 aaccgggacg aagaaaggtc aaacccgcgg cacgcggcga gggtcccgtg aacggtcgac
     7081 cgggcgcctg gcccggtacg cgtccgggtc cggcacggct tcccacaggc gggcatggtc
     7141 ctcacgcgcc cgcttgagcg tccggacgcc cttgggcgtg agccggtagt agatgcgcgg
     7201 acgcccgccc ttcgagcccg gaacgcgccg cgaggccttc ctcaggtagc cgccgtcggc
     7261 caggtggtcg agcgacaccc acaggcttcc cacggtcacg gactttcccg accgccgggc
     7321 gacctcgtcg cggatggtcg tcgagtacgc gtcgtcaccg aggcagagca cggcgacgag
     7381 caggatctcg tcggcccggg tcaggtgctt catgggcggg tgcgggagcg atcggaggcg
     7441 cggacatagt acgaaatcgt cgtaaccgtc gccgcgcccg attactcagg ccacgcgggg
     7501 caggtctccc ccgccgcgca cgggcagggc ggtcgacccc gcgaggtacc ggtccaccgc
     7561 gcgggcggcg tcgcgtccct cggcgatcgc ccacaccacg agcgactgcc cccgccgcat
     7621 gtcgccggcc gagaacacgc cgcggcggct cgtcgcgaag tccgtcgtca ctatgcggcc
     7681 gccgccgtcg aggtccagcc cgaaggcctc ggccagcgcg gcctccgcgc ccacgtagcc
     7741 catcgccagg agaaccaggt cggccggcca ccgccgctcc gtgccgtcga tccggacggc
     7801 gcggcggccc tcgaaggcga cgtccgccgt ctgcagggtc cgcacgcggg cctcgccctc
     7861 gaaggctgcg gtcatgacgg cccagagccg gtgggcccct tcctcgtggg acgaggtggt
     7921 cttcatcacg gccggcatga agggccaggg ctggcccgac ggacgctccg gcgggggcat
     7981 cggcatgagc tcgaactggg tcaccgacgc cgccccctgg cggttggcgg tccccacgca
     8041 gtcgctgccc gtgtcgccgc cgccgatcac gatgacgtgc ctcccggcgg cggaaatcgg
     8101 cacgcgagcc tgctcgcccc ccgcgacggc ccggttctgg cccgcgagaa agtcccaggc
     8161 gaagtggatg ccggccaggt cccgcccggg cacgggcagg tcgcgtggcc gcgtcgagcc
     8221 cgcgcacagg accacggcgt cgaagccggc gaggctggcc gcatccctga cctccacgcc
     8281 gcagcggaac gcgatccctt cctcctccat caggcggatg cggcggtcga gcacggcctt
     8341 gtccagcttg aagtcgggaa ttccgaaccg cagcagtccg ccgggccgct cgtggcgctc
     8401 gaaaaccgtg acctggtgcc cggcgcggtt cagctggtcc gcggccgcga gccccgaagg
     8461 gcccgagccg acgacggcca ccgagaaccc cgtgcgcacc gcggggggct gcggcacgat
     8521 ccacccctcg ctccatgcgc gctccacgat ggccagctcg atctgctcga tcgtcaccgg
     8581 atcggcggga atgccggcca cgcaggccga ttcgcacggc gccgggcaga tccggcccgt
     8641 gaattccggg aagttgttcg tggcgcgcag ccggagccag gcgcggtgcc agtcgtccgc
     8701 ggagacgagc gcgttccact cgggcaccag gttgccgagc gggcatccgg ccgtgcagaa
     8761 cgggatgccg caggccatgc accgacgggc ctgctccgcc acgacacccc cgggcgccgg
     8821 cacggccacc tcgtcgaaat ctcgcacgcg cgacgcgacc ggacggtacc gtacgggctg
     8881 ccgggcgatc tcgatgaagt cgaaggggcg attcatgccg ccaggccctc cgcgtccatg
     8941 cgctcgagcg cacggcggta ctcgtgcggc atcaccttca cgaactgggc gacggcctcg
     9001 tgccagtggt ccagcaccca ggtgccgacg gacgagtccg tccgatgcac gtgctcgcgc
     9061 accaggccct cgacgagcgc gatgtcgtcc ggctccgcga gcggctccac gtccaccatg
     9121 gaacggtcgc aacggccgct ccggaagtcg cgggtgccgt cgagcacgaa ggccgtaccc
     9181 ccgctcatgc cggccgcgaa attccggccc gtgggaccca gcacgagcac cgtgccgccc
     9241 gtcatgtact cgcacccgtg gtcgcccacg ccctcgacga cggccgtcgc cccgctgttg
     9301 cgcaccgcga accgctcgcc cgcgcgcccg cggaggaaca cgcgcccgga ggtcgccccg
     9361 tacagcgcga cgtttccgac gatgatctgc tcatggggcc ggaagcccgc gtcgtgcggc
     9421 gggcgaacct ggatgatcgc gcccgagagc cccttgccga agtagtcgtt ggcgccgccc
     9481 gtgagccaca gcgtgatgcc cttcgagccg aacgccaggc agctctgtcc cgcggtcccg
     9541 gcggcgtcga tcgtgatcgt gccgtcgggc agtccctcga ggccgcgccg ggaggcgatc
     9601 gcgtggctca ggagcgtccc gaacgtgcgg tccacgttgc gcacgcgggc ctcgagcgtc
     9661 acgggcgtgc cccgctcgat cgccgggcgc gcctcgtcca cgagccggtg gtccagcgcc
     9721 gagtgcacgt cccgcccgcc ggacggccgg tcgtgggacg cgacgtgctc cgggatcccg
     9781 ggccgcgcca ggatcggcgc gaggtcgagc gtgcgcgcct tcgggtgatc ctccggcacg
     9841 gcaggctcga gcaggtcggt gcggccggtc agctcgtcca gggtccggac gccgagcgag
     9901 gccatgatgc cgcgcagctc ctcggccacg aaatggaagt agttgatgac gtgctcggga
     9961 gcccccgc
//
LOCUS       DBNHQZ010000030         2086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_142550, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000030 DBNHQZ010000000
VERSION     DBNHQZ010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_142550"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16YU_00790"
     CDS             <1..326
                     /locus_tag="AC16YU_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063786.1"
                     /translation="YSLQRPRNDPDRLSQMLSGTLRLQPTENWDMSWRTSYDLEAKAF
                     NDHIIRLTRDLHRWQAHFDFMQTATGNWSFRFEVALTDNRDLKFDYRQRNLDVGLPAQ
                     GSGGS"
     gene            498..1628
                     /locus_tag="AC16YU_00795"
     CDS             498..1628
                     /locus_tag="AC16YU_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR6063787.1"
                     /translation="MRRVMFVALAALIGAAGCNNDSTVVVGAPAAPAGLEGSYYAGAI
                     TLTWELGSGWDGESFRVYGRRVSDADYFLIAEVTSCTSGLCSYSDTNIEASVTYEYYV
                     AAWDPGAGIETPSDFTVEVFSPERTAPPAPDQSRVIALDDANYIVWGDRSRDAADFSF
                     YRVYQAAPDGNDYLLGETDSEGFLDLLAGNGLTYDYFVTAVDVDGHEGASSAIASGTP
                     RPDYTGEILYDYFDDASLSGFRFQEDESASPILPGDDPDAHFSVERDGSGFWFLVPGP
                     AAGSRVHPNGVFTSALKCGVAADQTCLDATVAPTSGYTTNSVAIDPEYTYFLEVIGDD
                     GEYHYGAVRVVMQGENQDGGIIIFDWAYQLQPGNPELVPGRS"
     gene            1819..>2086
                     /locus_tag="AC16YU_00800"
     CDS             1819..>2086
                     /locus_tag="AC16YU_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019454.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR6063788.1"
                     /translation="MNPVLEAVPNISEGRDVAKVQRLVELVRSIGVDVLDWSSDADHH
                     RSVLTFIGEPSAVEEAAFTLARFALEHIDLRDHQGIHPRVGALDV"
BASE COUNT          339 a          708 c          702 g          337 t
ORIGIN      
        1 cgtacagcct ccagcggccc cggaacgatc cggaccggct gagtcagatg ctcagcggca
       61 ccctccggct ccagcccacc gagaactggg acatgagctg gcgaacgtcg tacgacctcg
      121 aggccaaggc gttcaacgac cacatcatcc gactcactcg cgaccttcac cgctggcagg
      181 cccacttcga cttcatgcag accgcgaccg gcaactggag cttccgcttc gaggtggccc
      241 tgaccgacaa ccgcgacctg aagttcgact accgtcagcg caacctcgac gtcgggcttc
      301 ccgcgcaagg aagcggcggc agctgacgct gtctcccatc cgcgcggcag gcgtcccctg
      361 tggaggacac gttgggcgac cgtgggtttc gtaacatatt gtgctgcaat gacttgagtg
      421 cacggcacgc gccgtgcttc tgcgttgggt gtcacgcaag gcctcgcgac cgcggggctc
      481 gaccgaggag aggaaccatg agacgtgtca tgttcgttgc gctcgccgcc ctgatcgggg
      541 ccgccggatg caacaacgat tcgacggtgg tggtgggcgc gcctgcggcg ccggccgggc
      601 tggagggcag ctattacgcc ggcgccatca cgctgacctg ggagctggga tccggttggg
      661 acggtgagtc cttccgtgtc tacggccgtc gggtgagcga tgccgactac ttcctgatcg
      721 ccgaggtgac gagctgcacg agcgggctgt gctcctacag cgacaccaac atcgaggcca
      781 gcgtcaccta cgagtactac gtggccgcct gggacccggg cgccggtatc gagacgccct
      841 cggacttcac ggtcgaggtg ttctcacccg agcgcaccgc gccgccggcg ccggaccaga
      901 gccgcgtcat cgcgttggac gatgcgaatt acatcgtctg gggggatcgc tcacgggacg
      961 ccgcggactt ctcgttctac cgcgtctacc aggccgcgcc ggacgggaac gactacctgc
     1021 tcggtgagac ggactcggag ggcttcctcg acctcctggc cgggaacggg ctgacctacg
     1081 actacttcgt gacggccgtc gacgtggacg ggcacgaggg cgcgtcgagc gccatcgcgt
     1141 cggggacgcc gcggccggat tacacgggcg agatcctgta cgactacttc gacgatgcct
     1201 cgctgtcggg cttccgcttc caggaggacg agtcggcgag ccccatcctg ccgggtgacg
     1261 accccgacgc gcacttcagc gtcgaacggg acggtagcgg gttctggttc ctcgtgcccg
     1321 gaccggccgc gggctcgcgg gtgcatccga acggtgtctt cacgagcgcc ctcaagtgcg
     1381 gcgtcgccgc ggaccagacc tgtctggacg cgacggtcgc gccgacgagc ggctacacga
     1441 ccaactcggt cgccatcgac cccgagtaca cgtacttcct cgaggtcatc ggtgacgacg
     1501 gggagtacca ctacggggcc gtccgggtgg tcatgcaggg cgagaaccag gacggcggca
     1561 tcatcatctt cgactgggcc taccagctcc agccggggaa cccggagctc gtgcccggca
     1621 ggtcctgacc gacgaaagca gacgaatcgg ggttgggcgc cgaccggacg cgagatacgc
     1681 gcggcgtcca gcccgccacg gccggctcat cgggatcgct cccggtgagc cggtttcgtc
     1741 ggcacggcgc ctcggggccg tcaccggcac cggttaccgc ggtaaccggc cggcggcatc
     1801 cggcttatct tgtccggcat gaatccggtt ctcgaagcgg tcccgaacat ctccgaagga
     1861 cgtgacgtcg cgaaggtcca gcgactcgtg gagctcgtgc gctccatcgg cgtcgacgtg
     1921 ctcgactggt cgtccgacgc ggaccatcac cgttccgtac tcacgttcat cggtgagccg
     1981 tcggcggtcg aggaggcggc gttcaccctg gctcgcttcg ccctggagca catcgatttg
     2041 cgggaccacc aggggatcca cccacgcgtg ggagcgctgg acgtcc
//
LOCUS       DBNHQZ010000031         3874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2988767, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000031 DBNHQZ010000000
VERSION     DBNHQZ010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2988767"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="AC16YU_00805"
     CDS             <1..549
                     /locus_tag="AC16YU_00805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR6063789.1"
                     /translation="ATKDYVWMNDALDLVRTAVDRGIPTFGSCWGHQMLARAFGGRVL
                     HDHARAEMGCHGVELTEAGLRDDLFSGFPARFLANMGHHDRVVTLPPGAVELAFNASQ
                     PNQAFRLDGAVAYGTQFHSELDAHRERERLIRYRSYYVRELAGEESFQAILASLAETT
                     EVDHLLHDFLVAFAADAPPSTP"
     gene            complement(560..1591)
                     /locus_tag="AC16YU_00810"
     CDS             complement(560..1591)
                     /locus_tag="AC16YU_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Acg family FMN-binding oxidoreductase"
                     /protein_id="HIR6063790.1"
                     /translation="MGRFSYVLDDPWRIREDDFPADAMPEDQLRFLLNYAVLAPSSHN
                     AQPWLFRLSGDEVALLADRMRALPVADPEDRELVMSCGAALLNLRVAIAHFGFSSEVR
                     TFPDLRDPDLLAFVRMGSRGRPDERDAGLFRCIPERRTFRFPFEDRPVGPDVAGRLEA
                     AAAREGATLALFDDPDRKNGLADLIAEGDRIQGGDRSFRRELASWIHPNRSKSRDGIP
                     GYGIGLGDLRSMSGPFVVRTFDLGRGRAAKDRELAEGSPLLGVLSTHADNPVSWLSAG
                     QALQRVLLTACERGLSASFLNQPVECPELWSRVGEISGGGEPQLILRLGPGKRVRPSP
                     RRSVMDVLS"
     gene            complement(1752..2531)
                     /locus_tag="AC16YU_00815"
     CDS             complement(1752..2531)
                     /locus_tag="AC16YU_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6063791.1"
                     /translation="MKTPQYKDREPVRFSGGAENASGGRRGIPGRRKLHRAPGTDLRR
                     DYRLTIQVGLVVSLFAVTALVRAPLTPADEGFDLTLADQETVQMEEILQTAQVEKPPP
                     PPRPPVPVEVPNDEILMDEDLDLDASLDLDEPLFDLPPPPPTPAPEQEEDDLEQEIFV
                     VVEQMPEIIGGTQKVYEYLEYPPIARQAGMEGLVVIQVVVNQDGVPVEPVVARSAGEV
                     LDRAAIEAVMKLRFVPGKQRGKPVRVRMAIPIRFRLRDVQG"
     gene            complement(2542..3513)
                     /locus_tag="AC16YU_00820"
     CDS             complement(2542..3513)
                     /locus_tag="AC16YU_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6063792.1"
                     /translation="MKRMLTVRTILAATDFSGCADAAVDLALDVARRHRASLRLLHVE
                     EAGGPTSQEFGGLHPLSRAWENDLVGSVRPSEIQMVWDDVPIVAEVRSGIEATDAILD
                     AVASYDVDLLVMGTHGQHSVRRFLNDTPDAWSIGLTAREVLSETRCPVLAVGPRHDHP
                     VGPPQEVVAGVDDSPLADEVLTNAAALASFYGTPLIIVHVVPYGTDPTRVLEMRRDLV
                     ARAKALIPDVVPFEVHTPEGRPAETLVRLVRHRPGALLVVASHGHRTRGSVRIGSVAD
                     AVVRSAPCPVITIKPYGKSLVTPDPEVESRLARVTTRLPGAATARPA"
     gene            complement(3510..>3874)
                     /locus_tag="AC16YU_00825"
     CDS             complement(3510..>3874)
                     /locus_tag="AC16YU_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6063793.1"
                     /translation="LHPAFYNAGAFSIYDLQPDVELRALEHMRLEFSAAPGPDVPVTF
                     EVTSGHAAVEIANMTRDLPVGLVVMATHGLTGFAHLFLGSVAEHVARISAIPVFSVKS
                     FGRDLFVDSPEKREMKHA"
BASE COUNT          602 a         1361 c         1323 g          588 t
ORIGIN      
        1 gccacgaagg actacgtgtg gatgaacgat gccctggacc tggtccggac ggcggtggac
       61 cgtggcattc cgacgttcgg gtcctgctgg ggtcaccaga tgctcgcgcg cgcgttcggg
      121 ggccgtgtcc tgcatgacca cgcccgtgcc gagatggggt gtcacggggt ggagctgacc
      181 gaggccggac tccgcgacga cctgttctcg gggtttccgg cgcggttcct ggccaacatg
      241 gggcaccacg accgcgtggt cacgctgcct ccgggcgccg tggagctcgc gttcaacgca
      301 tcgcagccca accaggcctt ccggctcgac ggcgcggtcg cgtacggcac ccagttccat
      361 tccgaactcg acgcccaccg ggagcgggag cgcctgatcc gctaccggtc ctattacgtt
      421 cgggagctcg caggcgagga atcgttccag gccatcctgg catcgctcgc ggagacgacc
      481 gaagtggacc acctcctgca cgacttcctg gtggccttcg ccgccgatgc ccccccgtcc
      541 acgccctgac gcggctcgct cacgagagga cgtccatcac cgaccgccgc ggcgacggtc
      601 gcacccgttt tccgggaccg aggcgcagga tcagctgcgg ctcgcctcct ccggaaatct
      661 cccccacccg ggaccagagc tcggggcatt cgacgggctg gttcaggaac gacgcggaga
      721 gacccctctc gcaggccgtg agcagcacgc gctgaagcgc ctggccggcc gaaagccagg
      781 agacgggatt gtcggcgtgg gttgacagca cgccgaggag cggcgagccc tcggcgagct
      841 cgcgatcctt cgcggcacgg ccgcggccca ggtcgaaggt ccgcacgacg aacgggccgc
      901 tcatcgaacg gagatcgccg agtccgatcc cgtagcccgg gatgccgtcc cggctcttcg
      961 accggttggg gtggatccac gaggccagct cgcgccggaa cgatcggtcg ccgccctgga
     1021 tgcggtctcc ctcggcgatg aggtcggcca ggccattctt gcgatcggga tcgtcgaaca
     1081 gggcgagggt cgcgccctcc cgggcggcgg cggcctcgag ccggcccgcc acgtccggcc
     1141 ccaccggccg gtcctcgaag ggaaaccgga acgtccgtcg ctccgggatg caccggaaca
     1201 ggccggcgtc cctttcgtcg ggcctcccgc gggagcccat ccgcacgaag gccagcaggt
     1261 ccgggtcgcg caggtcggga aacgtccgca cctcggacga gaagccgaag tgcgcgatcg
     1321 ccacgcgcag gttcagcagg gccgcgccgc agctcatgac cagctctcgg tcctccgggt
     1381 cggccacggg cagcgcccgc atccggtcgg cgagaagcgc cacctcgtcg ccggaaaggc
     1441 ggaacagcca cggctgggcg ttgtgcgagg agggcgcgag gaccgcgtag ttcaggagga
     1501 aacgcagctg gtcctccggc atggcgtccg ccgggaagtc gtcttcccgg atccgccatg
     1561 gatcgtcgag gacgtagctg aatcgaccca tccgcagcct gcctggaatg cggacgccag
     1621 cgcccgcggc cgtgaaggaa cggcgaagga tagggcgccg cccgcccccc tgtctgcgcg
     1681 ggcgccacgc acaccgttgt agggtatatc cctacgacgt ccgtcctctc cgcgcctcgc
     1741 tgtccgacag tctagccctg cacatcgcgg agccggaagc ggatcgggat ggccattcgc
     1801 acgcggaccg gcttgccccg ctgcttcccc gggacgaacc gcagcttcat caccgcctcg
     1861 atcgccgcac gatccagcac ttcgccggcc gaccgggcga ccaccggctc cacgggcacg
     1921 ccgtcctggt tcacgacgac ctggatcacc acgagcccct ccatgccggc ctgccgggcg
     1981 atcggagggt attcgagata ctcgtagact ttctgggtgc cgccgatgat ctccggcatc
     2041 tgctcgacca ccacgaaaat ctcctgctcc agatcatcct cctcctgctc tggcgcgggg
     2101 gtcggcggag gcggcggaag atcgaaaagc ggctcgtcca gatcgagcga cgcatccagg
     2161 tccaggtcct cgtccatcag gatctcgtcg ttcggcacct ctaccgggac gggcggacgc
     2221 ggcggcggcg gaggcttctc gacctgcgcg gtctggagga tttcctccat ctgcacggtc
     2281 tcctggtccg caagggtcag atcgaacccc tcgtcagccg gcgtgagcgg cgcacgtacg
     2341 agtgccgtga ccgcgaagag tgacacgacc agaccgacct gtatggtcag gcggtagtcc
     2401 cggcgaaggt ccgttccggg ggcccggtgt agcttgcgcc gaccggggat tccccgtcgt
     2461 ccaccggagg cgttctcggc gccgccgctg aaccggaccg gctctctgtc cttgtactgg
     2521 ggcgtcttca tggtgtaccc ttcacgcggg tcgagctgtc gccgctcccg ggagccgcgt
     2581 ggtcacgcgg gccagccgcg attcgacctc gggatccggg gtgacgagtg attttccgta
     2641 cggtttgatc gtgatcaccg gacacggcgc gctccgcacg acggcgtccg ccacgctccc
     2701 tatcctcacc gagccccgcg tcctgtggcc gtgcgaggcg accacgagca gggctccggg
     2761 acgatgccgg accaggcgga ccagggtctc cgccggccgg ccctcgggcg tgtggacttc
     2821 gaacggcacg acgtccggaa tgagtgcctt ggcacgggcg acgaggtccc tccgcatttc
     2881 caggacgcgg gtggggtccg tcccgtacgg aaccacgtgc acgatgatga gcggggttcc
     2941 gtaaaacgag gccagcgccg cggcattcgt gagcacctcg tccgcgagcg gcgaatcgtc
     3001 gacgcccgcg accacttcct gcggagggcc gaccggatgg tcgtggcgcg gaccgacggc
     3061 gagcacggga cagcgcgtct ccgagagcac ctcccgggcc gtcaggccga tggaccacgc
     3121 gtcgggcgtg tcgttcagga agcgtcgcac gctgtgctgc ccgtgggtgc ccatgaccag
     3181 caggtcgacg tcgtaggacg cgacggcatc caggatggca tccgtcgcct cgatgccgct
     3241 ccggacctcg gcaacgatcg gtacatcatc ccagaccatt tggatctcgg acggtcgaac
     3301 ggagccgacg aggtcgttct cccacgcacg ggacagcggg tgcaggccgc cgaactcctg
     3361 gctcgtcggc ccgccggcct cctcgacgtg caggagccga agcgaggcac ggtgccgccg
     3421 tgccacgtcc agcgccaggt cgaccgctgc gtcggcgcag cccgagaagt cggtcgctgc
     3481 cagtatggtc ctcacggtca gcatgcgctt catgcgtgtt tcatctcccg cttttccggt
     3541 gagtccacga acaggtcccg tccgaagctc ttcacgctga agaccgggat ggccgatatc
     3601 cgcgcgacgt gctcggcgac gctcccgagg aacaggtgag cgaaccccgt gagcccgtgg
     3661 gtggccatga ccaccagccc gaccgggagg tcgcgggtca tgttggcgat ctcgacggcg
     3721 gcgtgacccg aggtgacctc gaacgtgacg ggcacgtccg gacccggcgc tgccgagaac
     3781 tcgagcctca tgtgttcgag cgcccggagc tcgacgtccg gctgcagatc gtaaatcgag
     3841 aacgccccgg cgttgtagaa cgccggatgc aggg
//
LOCUS       DBNHQZ010000032         6997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1770151, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000032 DBNHQZ010000000
VERSION     DBNHQZ010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1770151"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="AC16YU_00830"
     CDS             complement(<1..1334)
                     /locus_tag="AC16YU_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063794.1"
                     /translation="MEAILLVEKRIGARVSIQARTAGALATAVATRARSFSDGLSAAG
                     YAFGRAVSGRRAAVILDPDGLRSSEAVVSDAVRARTPLVVHLVCHDDWSAALSAGAAG
                     AVVLVTPDIRDVAALTVAAHRMAEDVLLPVICVHDSSRIALASADIPEPSDEALAALL
                     PDRVELTGWQRRLFEAERPAVPEWLSPDRPLGIGARLDGIDDVAAAAGRELFLDAGLG
                     ERVDRSLAVVSDALGRSIRGLRTFGSGKGAPWIVTAGPLAGLAAELAGRAGHPVRSAA
                     FVRLVPFPSEPFASAALDGPAALLLAAHPGLLADSIARAAREASGRRVLLGLFAGGSG
                     LPSTADLEAVAGAVRSGAREGPVFVGAMRPQDRPGIPEAERLEQLLRAERPDVDRRLL
                     HAPRPEADVAFCIATGPILSDLLQDEAAQAIAGTTGRPVRTVSLRTHVEAPAS"
     gene            complement(1381..2247)
                     /locus_tag="AC16YU_00835"
     CDS             complement(1381..2247)
                     /locus_tag="AC16YU_00835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR6063795.1"
                     /translation="MAALTAIGYLAALCVTLAVVLAIAQRTLYVEEDPRIDDVTDLLP
                     GANCGACGLPGCRAFAEQLVGGAVQPGECTVSDPDARGTIASYLGIEVGSVERRVARL
                     LCAGGEDVAGHAGAYAGFSSCRAAATIAGGPKECTYGCLRLADCREACTFDAITMGPT
                     GLPVVDLEKCTACGDCVDACPKGLFVIMPVGRRLVVQCRSLLEGDAALATCNVACTGC
                     GLCAADAPEGLIRMEHGLPVIQPERWELETPIATLRCPTGAITWVEGQQFPEVFEAMN
                     PTSTRVRDAVQA"
     gene            complement(2251..4665)
                     /locus_tag="AC16YU_00840"
     CDS             complement(2251..4665)
                     /locus_tag="AC16YU_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S domain-containing protein"
                     /protein_id="HIR6063796.1"
                     /translation="MEAIETHSSGADGTAVAETDPAAPASHVLRRLPAMAAPADPAAT
                     ARALQQFFRTGTLPPDSGLSAGLSGHVPALLFPFLGRPGLRTPWPIVLDESVDAPLPV
                     TLSAFMDRVADAVCDDGDEGELRRRFLARVEEAIRTAVLPEESNLEEAWWSAVDAVRP
                     GIRTDRRDAFEEGIRAAAAHVPDTARLLACSAAAPVALARDAARRLVGEISGPVAEEA
                     DRLRLALQNLLDVEREESPGAVEPGRLRGAAGGALTDAFDFDALSHLLDEAPHAAGLP
                     PERRERMEARAASLSEHVQALYGRSGTVLRPVSLRSAREAVAERTGRLLALFRDLEAA
                     RLEASHRYRPAIHDPYLEALTPARLSPEDLRRCPPLIVTADEATLDAAGIGHLLELLA
                     GTAPVKVLLVLRGSADDPESPSAACSLPAAAMGHGLAFVVQASLSDIVTLSRGIVDAV
                     RHDGPALVAVHAGAAKSGLPAYFASAVARDGRCAPSFVFAPGIGKPWADRLSVDGNDD
                     PEADWTVASLRIESGEGGEEISTAFTWLDHALTRPALAGYFLPVEPDLDLDLLLPADE
                     FLALGPEDRAGKIPFVPCATTDGFLVKAVTARPLVCAAERALDRWRRLRELAGIGNSY
                     AERAVDAVRGEIGAERDAAIRAAEAAHAEALDRTIASVAESVVSNIAASLLDLPSGAP
                     APALRPAPAAATPAAAPGPTPEESAPAPPVAEEEPEESLSLDEAYIETVRCTSCNECT
                     NLNGQIFAYNGEKQAYIKDASAGPYRDLVTAAEKCPVRIIHPGKPLNPDEPGLDELVE
                     RAKPFL"
     gene            complement(4676..5638)
                     /locus_tag="AC16YU_00845"
     CDS             complement(4676..5638)
                     /locus_tag="AC16YU_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6063797.1"
                     /translation="MFGLQDNATVDVGGDYYSLQDYQGAVPRWCPGCGDNTILSSVQK
                     LLRDEQLPPEKTVFVSGIGCSSRFPHHMGTYGFHSLHGRALPIAQGVKIRRPDLKVFV
                     NTGDGDCCSIGTAHWIHAIRYNFDMVVMVHDNEVYGMTKMQASPTTPKGTRTNTTPKG
                     AFLDPLNILSATLGVSNVSFVANVVEWIPEVMYQVLREAYHHKGFSFVRIVQRCPHFL
                     PAMFDPLLQDPNKILLLTDERGLKPSDDLRKVYRNEQAHDPSDLDAARRIVQVQDPMP
                     VGVLYRNEDVPVYDEIRRPARVVTHELRRQALDREFDRFAVDLA"
     gene            complement(5642..>6997)
                     /locus_tag="AC16YU_00850"
     CDS             complement(5642..>6997)
                     /locus_tag="AC16YU_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6063798.1"
                     /translation="VYNRNMDFARDTIRYIFRKKSQKVVDSNISLLDAGYEWGVENVD
                     LSYEIPPMPDKGRQVVINGNQAIALGVVASGMEVCAMYPITPATSASHYLSEIFDNVG
                     GVLHQAEDEIAAAAVAVGASYAGKCAVTITSGPGMSLKTEVLGLASMAEIPLVVVNVQ
                     RGGPSTGLPTKTEQGDLLQAIFGTHGDAPKVVMAPATIEDCFYSVITARRIAETFRMP
                     VYVLSDANLATGQAPFPRPEYRAEWVAPPIDQRPVPKGTKPYDWDEKTGLSPRLIPGQ
                     PGGMYAVTGLAHTDTAKVAYTPDANQEGSRRRSLKLAALQRTLKTPTVNGDEEGDLLI
                     VGWGSTKGAIEEAIDRVREDGGSVSSLHLTFLQPLAPGIGEILKRFKRVMTVEINYSD
                     SLEDELITDENRRYSNLSWLLRARYLVDVQNWTNVHGQPIGPGKIEEMIREQLAVLQP
                     A"
BASE COUNT         1029 a         2413 c         2573 g          982 t
ORIGIN      
        1 gaggccggag cctcgacatg ggtccgcagc gagaccgtgc gaaccgggcg cccggtggtt
       61 cctgcgatcg cctgggccgc ttcgtcctgg aggagatccg acaggatcgg acccgtggcg
      121 atgcagaagg cgacgtccgc ctctggacgc ggagcgtgga ggagtcgacg atccacgtcc
      181 gggcgctctg cacgcagcag ctgctcgagc cgctcggcct ccgggattcc gggccggtcc
      241 tgtggccgca tggcgccgac gaacaccgga ccctccctgg cgccggagcg gacggccccg
      301 gccacggcct ccaggtcagc cgtcgaggga agaccgcttc caccggcgaa caaccccagg
      361 agcacccgac gtccggaggc ctcccgggcg gcccgggcga tcgaatccgc gagcagtccg
      421 ggatgggcgg cgagcaggag ggccgccggg ccgtccaggg ccgccgacgc gaacggctcc
      481 gacgggaacg gaaccaggcg gacgaatgcg gccgagcgga ccgggtgccc cgcgcgaccg
      541 gcgagctccg cggccagccc ggcgagcggg ccggccgtca cgatccaggg cgcccccttg
      601 cccgagccga acgtgcggag accccgtatg gagcgcccga gcgcgtcgga tacgaccgcc
      661 agcgagcggt ccacccgctc gcccaggccc gcgtccagga acagctcgcg cccggcggcg
      721 gcagccacgt cgtcaatgcc gtccagccgt gcgccgatgc cgagcggccg gtcggggctc
      781 agccactcgg gcacggcggg tctctcggcc tcgaacaggc gccgttgcca gccggtgagc
      841 tcgacgcggt ccggaaggag tgcggcaagg gcctcgtccg agggctcggg aatgtcggcc
      901 gaggcgagcg cgatgcgcga cgagtcgtgc acgcagatca cggggagcag tacgtcttcg
      961 gccatgcgat gggccgcgac ggtgagtgcg gcgacatccc tgatgtccgg cgtcacgagc
     1021 acgaccgcac ccgcggcgcc ggccgagagg gcggcggacc agtcgtcgtg gcagacgagg
     1081 tggacgacga gcggagtccg ggcgcgcacg gcgtccgaaa cgaccgcttc ggaggaacgg
     1141 agtccgtccg ggtccaggat gacggccgcg cgccggcccg agacggcgcg cccgaacgca
     1201 tacccggcgg ccgaaaggcc gtccgagaag gaccgggccc gcgtggcgac ggccgtggcg
     1261 agggcgcccg cggtccgggc ctggatggac acccgcgcgc cgatccgctt ttcgacgaga
     1321 aggatcgctt ccatgccgaa ccggacgggg ccggccggac cggatgctgc gggtattcgc
     1381 ttacgcctga acagcatcac ggaccctggt cgaagtgggg ttcatcgcct cgaagacctc
     1441 cgggaactgc tgtccctcca cccacgtgat cgccccggtg gggcagcgca gcgtcgcgat
     1501 gggggtctcc agctcccagc gctcgggctg gatcacgggc aggccgtgtt ccatccggat
     1561 aaggccctcg ggagcatcgg ccgcacacag cccgcagccg gtgcacgcga cgttgcaggt
     1621 tgccagcgca gcgtcgccct cgagcagcga gcggcactgc acgacgagcc gcctgccgac
     1681 cggcatgatc acgaacaggc ccttcgggca ggcgtccacg cagtccccgc aggcggtgca
     1741 cttctccagg tcgaccacgg gcaggcctgt cggccccatc gtgatggcat cgaacgtgca
     1801 ggcctccctg cagtcggcga gccgaaggca gccgtaggtg cactccttgg gtccgccggc
     1861 gatggtcgcc gccgcgcggc aggacgagaa gccggcgtag gctccggcat ggccggcgac
     1921 gtcctcaccc ccggcacaga gcagtcgggc gacgcgccgc tccaccgagc ccacctcgat
     1981 gcccaggtac gaggcgatcg ttccccgggc gtccggatcg gagacggtgc actctcccgg
     2041 ctggacggcg ccgcccacca gctgctcggc gaaggcccgg cagcccggca gtccgcacgc
     2101 gccgcagttc gctcccggca ggaggtccgt gacgtcatcg atgcgcggat cctcttccac
     2161 gtagagcgtc cgctgcgcga tggccaggac gacggccagg gtcacgcaca gcgctgccag
     2221 gtagcctatg gcggtgagtg cggccatgga ttacagaaac ggtttggcgc gctccacgag
     2281 ctcgtccaga cccggctcgt cgggattgag gggcttgccc ggatggatga tccggaccgg
     2341 gcacttttcc gcggccgtca ccaggtcgcg gtacggaccg gcggacgcgt ccttgatgta
     2401 ggcctgcttc tcgccgttgt aggcgaagat ctggccgttc aggttggtgc actcgttgca
     2461 cgacgtgcag cggaccgtct cgatgtaggc ctcgtccagc gagagcgatt cctcgggttc
     2521 ttcctcggcg acaggcgggg ccggagcgct ttcctccggg gtgggccccg gggcggcggc
     2581 cggcgtcgcg gcggccggtg ccgggcgcag cgctggagcc ggcgcacccg agggcaggtc
     2641 cagcagcgac gcggcgatgt tcgagacgac cgactcggcg accgaggcga tggtgcggtc
     2701 gagcgcttcg gcatgcgccg cctcggcggc ccggatggcc gcgtcgcgtt ccgccccgat
     2761 ctcgccgcgc accgcgtcca cggcccgctc cgcgtaggag ttgccgatgc cggccagctc
     2821 gcgcaggcgt cgccagcggt cgagcgcccg ctcggcggcg cacacgaggg gtcgtgcggt
     2881 cacggccttg accaggaagc cgtcggtcgt ggcacacggg acgaacggga tcttcccggc
     2941 gcgatcctcc ggaccgaggg cgaggaactc gtcggcgggg agcagaaggt ccaggtcgag
     3001 gtccggctcg acgggcagga agtacccggc gagcgccggc cgcgtcagcg cgtggtcgag
     3061 ccacgtgaag gccgtggata tctcctcacc gccttccccg gactcgatgc ggaggctcgc
     3121 gacggtccag tccgcctccg ggtcgtcgtt tccgtccacg gagagccggt ccgcccacgg
     3181 ctttccgatc ccgggtgcga agacgaagga cggcgcgcac cggccgtccc gagcgacggc
     3241 cgaggcgaag tacgcgggca ggccactctt cgcggcgccc gcgtggacgg cgacgagcgc
     3301 gggtccgtcg tgccgcacgg catcgacgat gccccgcgac agcgtcacga tgtccgagag
     3361 cgacgcctgg accacgaagg ccaggccgtg acccatggcg gcggcaggca ggctgcaggc
     3421 cgcggacggc gactccgggt cgtcggccga gccgcgcagg acgagcagga ccttgaccgg
     3481 cgcggtgccg gcgaggagtt ccagcaggtg cccgatgccc gcggcgtcga gggtcgcctc
     3541 gtcggccgtc acgatgagcg gcgggcaccg tcgcaggtct tccggcgaca gcctggccgg
     3601 ggtcagcgcc tcgaggtacg ggtcgtggat cgcgggccgg tagcggtggg acgcctcgag
     3661 tcgcgcggcc tccaggtcgc ggaagagcgc gagcagtcgc ccggtccgct ccgccacggc
     3721 ctcgcgggcc gaccgcaggg acacggggcg cagcacggtg ccggaccttc cgtacagggc
     3781 ctggacgtgc tccgagagcg aggcggcccg ggcttccatg cgctcccggc gctccggcgg
     3841 aaggccggcg gcgtggggcg cttcgtccag gaggtgcgac agggcgtcga aatcgaacgc
     3901 gtcggtgagg gcgcctccgg cggctccgcg gagccgcccg ggctcgacgg cgccggggga
     3961 ttcctcgcgc tccacgtcca ggaggttctg cagtgcgaga cggagccggt ccgcctcctc
     4021 ggccacgggg cccgagattt cgccgacgag acgccgcgcc gcatcccggg ccagggccac
     4081 tggagccgcc gcggagcagg cgagaagccg cgcggtatcc ggaacgtgag cggccgccgc
     4141 ccggatgccc tcctcgaagg cgtcgcgccg gtcggtgcgg atgcccggcc ggacggcatc
     4201 gaccgccgac caccacgcct cctccaggtt cgattcttcc ggaaggacgg ccgtgcggat
     4261 ggcctcctcc acgcgggcca ggaaccgccg gcggagctcg ccctcgtccc cgtcgtcgca
     4321 gacggcgtcg gcgacccggt ccatgaaggc agagagcgtc accgggaggg gggcatcgac
     4381 cgactcgtcg agcacgatcg gccacggagt ccggaggccg ggccgcccca ggaacggaaa
     4441 gagcagggcc ggaacgtgtc cggacaggcc cgcggacagc ccggagtcgg gcggaagcgt
     4501 gccggtccgg aagaactgct gcagggcccg ggccgtcgcg gcggggtccg ccggagccgc
     4561 cattgcgggc agccggcgca gcacgtggct ggccggagcc gccgggtcgg tctccgcgac
     4621 cgccgtgccg tcggctccag agctatgggt ctcgatcgct tccacgggat cgggttcagg
     4681 cgaggtcaac ggcgaaacgg tcgaactcac ggtccagcgc ctggcggcgc agctcgtgcg
     4741 tcaccacgcg cgcggggcgc cggatctcgt cgtacacggg aacgtcctcg ttccggtaca
     4801 gcacgccgac cggcatggga tcctgcacct ggacgatccg ccgcgcggcg tccaggtccg
     4861 aggggtcgtg cgcctgctcg ttcctgtaga ccttgcgcag gtcgtccgac ggcttgagcc
     4921 cgcgctcgtc cgtcagcagc aggatcttgt tcggatcctg aagcagcgga tcgaacatcg
     4981 cgggcaggaa gtgcgggcag cgctgcacga tgcgcacgaa cgagaacccc ttgtgatggt
     5041 aggcctccct gaggacctgg tacatcacct cgggaatcca ctcgaccacg ttcgctacga
     5101 acgacacgtt cgaaacgccg agcgtggcgc tcaggatgtt cagcggatcc aggaaggcac
     5161 ccttcggcgt ggtgttcgtg cgggtcccct tcggcgtggt gggggaggcc tgcatcttgg
     5221 tcatcccgta cacctcgttg tcgtgaacca tcacgaccat gtcgaaattg taccggatgg
     5281 cgtgaatcca gtgtgccgtg ccgatggagc agcagtcgcc gtcgcccgtg ttcacgaaca
     5341 ccttcaggtc cggccgccgg atcttcacgc cctgggcgat gggaagcgcc cggccgtgca
     5401 ggctgtggaa tccgtacgtg cccatgtggt gcggaaaccg gctggagcac ccgatgcccg
     5461 agacgaagac ggtcttctcg ggcgggagct gctcgtcgcg cagcagcttc tgcacgctgg
     5521 acaggatcgt gttgtcgccg cagcccgggc accagcgcgg cacggcgccc tggtaatcct
     5581 ggaggctgta atagtcgccg ccgacgtcca ccgtggcgtt gtcttgcagt ccgaacatgg
     5641 gtcaggcggg ttgaagtacg gccagctgct cgcggatcat ctcctcgatc ttgccgggtc
     5701 cgatgggttg accgtggaca ttggtccagt tctgtacgtc cacgaggtag cgggcgcgca
     5761 ggagccacga gaggttcgag tagcgccggt tctcgtcggt gatcagctcg tcctcgagcg
     5821 agtccgagta gttgatctcg accgtcatga cgcgcttgaa gcgcttcagg atctccccga
     5881 tgcccggggc gaggggctga aggaaggtca ggtgcagcga ggagacgctg ccgccgtcct
     5941 cgcggacgcg gtcgatggcc tcctcgatgg cccccttcgt cgagccccag ccgacgatga
     6001 gcaggtcgcc ctcctcgtcg ccgttcacgg tcggggtctt gagcgtccgc tggagggcgg
     6061 cgagcttgag gctgcgccgc ctggagccct cctggttggc gtcgggcgtg taggcgacct
     6121 tggccgtgtc cgtgtgcgcg agacccgtca cggcatacat gccgcccggc tgcccgggga
     6181 tgagccgcgg agagagaccg gtcttctcgt cccagtcgta cggcttggtg cccttgggca
     6241 ccgggcgctg gtcgatcggt ggagccaccc actcggcccg gtactcgggt cgcgggaacg
     6301 gagcctggcc ggtggccagg ttcgcgtcgg agagcacgta caccggcatg cggaaggtct
     6361 ccgcgatgcg gcgcgccgtg atgaccgagt agaagcagtc ctcgatggtc gccggggcca
     6421 tgaccacctt cggcgcatcg ccgtgcgtgc cgaagatcgc ctgcagcagg tcgccctgtt
     6481 ccgtcttggt cgggaggccg gtcgaggggc cgccccgctg cacgttcacg accaccagcg
     6541 ggatctcggc catggacgcg aggcccagga cctcggtctt cagcgacatg cccggtccgg
     6601 acgtgatcgt gaccgcacac ttgccggcgt acgacgcgcc taccgcgacg gccgcggccg
     6661 cgatctcgtc ctcggcctgg tgcagcacgc cgcccacgtt gtcgaagatc tcgctcaggt
     6721 agtgcgaggc ggacgtggcc ggcgtgatgg gatacatcgc gcagacctcc atcccggagg
     6781 ccacgacgcc gagggcgatc gcctggttgc cgttgatgac gacctggcgc cccttgtccg
     6841 gcatcggcgg tatctcgtac gaaaggtcga cgttctcgac cccccactcg taccccgcgt
     6901 cgagcagcga gatgttcgag tcgacgacct tctgcgactt cttccggaag atgtagcgga
     6961 tcgtgtcgcg ggcgaagtcc atgttccggt tgtagac
//
LOCUS       DBNHQZ010000033         9774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_550243, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000033 DBNHQZ010000000
VERSION     DBNHQZ010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_550243"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="AC16YU_00855"
     CDS             complement(<1..1328)
                     /locus_tag="AC16YU_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6063799.1"
                     /translation="MNRPERRPRGAWWWVPSLYFAEGLPYVVVMIVSVIMYKRLGISN
                     TEIALYTSWLYLPWVIKPLWSPIVDILRTKRFWIVTMQLLIGAGLAGVALTLPTGDFF
                     RYSLAFLWLLAFSSATHDIAADGFYMLGLSQHDQTWFVGIRSTFYRLAMITGQGLLVI
                     LAGYLETATGLDPLAVSVTADASIEFVEAAPPDTLGAAADRGAARLESSAEEYRVAAR
                     PVDAAYADSLIALAHAWNLRHGFTEAPATVSGGDDREEPGWWARSVSGPLGRTIQRWF
                     GEDVPVPSPHSRSGNVVLVPLRVSGAVPPGDEVAVNVAHEGGDPSLRIVEGERLSFRA
                     ENAGAAALVAVRLDPKLDHTTRATFRARSGNIPLAWSITFMVLTGLFTLFVVWHRFVL
                     PRPPEDRPTAEASAASVLREFGATFVSFFRRPRIGIVLAFLLLYRFAEAQL"
     gene            complement(1325..4300)
                     /locus_tag="AC16YU_00860"
     CDS             complement(1325..4300)
                     /locus_tag="AC16YU_00860"
                     /inference="COORDINATES: protein motif:HMM:NF051532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="golvesin C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR6063800.1"
                     /translation="MVLAACGGSGVVMEPPPEPWTVERVSDAGRAAAESAIAACRLED
                     CGFRLGPPAAVREAAFDPVTGTLTVELNRAAGETAWREDGVRALRQAVASRIAPDAPV
                     EHIALRALDLPLEELVPNLYRSSLAPDASRRPGTRPEPRPLVRRPDGPPPPGRGLHGR
                     HVALWPSHGWYYERNLDRWEWQRARLFSTVEDVLPASFVLPFLVPMLERAGATVWLPR
                     ERDTQPRLVVVDNDGSDGRSGYRETRGWMRGPGAGFATGTPPYGAGHNPFRQGTWRVA
                     DMAPGSQDSVSWIPEIEASGEYSVHVSYGDDPRATDRAEYIVRHAGGETRLSVDQTMA
                     ERTWVYVGTFRFLEGAGADSASVVLRATGPAGRVVSADAVRFGGGTGLVERGGTTSGR
                     PRWLEAARYHLQYSGMPDTLVYHVTGELDDYGDDYRARGEWVNFLRGAPFGPNKDRGA
                     EGLGVPVDLSLAFHTDAGVAGADTTIGTLLIYSSEGADSTRVFPDGMSRVANRDFADI
                     LQTQLVSDLRALHDSAWARRPLWDRDYSEAFRPNVPAALLELLSHQNFADMRHALDPA
                     FRFDAARSIYKAMLRFLAAQYGEPDPVVQPLPPDHLEARVSDGVVILRWRPVPDPLEP
                     TALPDRYIVYTRIGEGGYDGGRVVDTTEARIVVEAGRDYGFRVAGLNDGGEGFPSEEV
                     AAAWVPGRPRVLIVSGFDRVAPPAAIDRPPFRGFLDLLDHGVALGMDLSFTGAQHEFD
                     TAVPWRDDDDPGHGASWGTHETMVFPGNTRDFPLVHGRSIRAAGYGYDTVSDERLAAE
                     PELVAGHAIVDVLLGEERETTPGHAPVSAFRAFPPLLMDALEGAAAAGVGLLVSGSYV
                     GTELMAGESDPRSAFAREVLGIRPRTGNAVSTGDVVPAGDGAVPDPGPFSFHSERNPG
                     SYEAEAPDAVEPADDRGRTILRYGETNMSAAVAFRSDHVAVTLGFPFETIAGREARDR
                     VMAALLEFLSHPATQPADR"
     gene            complement(4341..5903)
                     /locus_tag="AC16YU_00865"
     CDS             complement(4341..5903)
                     /locus_tag="AC16YU_00865"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10-like protein"
                     /protein_id="HIR6063801.1"
                     /translation="MIAPSDPSRSRPARRSARALGSTVVLALLVLAGCAGPRGAATPG
                     DEGAAPATAAAPSAMREFRGIWVATVDNIDWPSEPGLDPARQRDELLALLDQAAALRM
                     NAVIFQVRPMADAVYESELEPASWYVSGEQGRGPGYDVLAFAVEEAHRRGLELHAWFN
                     PFRAGHPTMRGTYDSSHVSIRHPEWVVRYGEQLWIDPGSPAASAHSLAVFEDVVRRYD
                     VDGVHMDDYFYPYPVVDATGIPVPFPDSTSRRMAEEAGTTLALADWRRANVDDFVERL
                     YGMVKRVKPWVKVGISPFGIWRPGHPEGVTGFDQYDQIYADARLWLQEGWLDYFTPQI
                     YWAVDSQGQPYGRLLDWWAGENTAARHLWPGNFTSRVIPAGGASWGPDEIVRQVRLTR
                     EQAGATGNVHFSMRALEPGAGVDTVLAPALYREPALVPATGWLGGLAPGAPEARLRRL
                     GDRSILDLAPGGGGEPFLWHLRTLRGAEWSVELVPAWRRALELGPRPPDAVVVSAVNR
                     LGQEGPDVRVLP"
     gene            complement(5958..6947)
                     /locus_tag="AC16YU_00870"
     CDS             complement(5958..6947)
                     /locus_tag="AC16YU_00870"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR6063802.1"
                     /translation="MSPAESPSTLRSEGIPIPTEGPCLLAHGGAWDIPDAYLDEQREA
                     LLRAVEAGRAELESGGKAVDVATLVVAAMESAGVFDAGCGAVLTRKGTVELDAGVMDG
                     ESLAFGSVAAVHRLGNPIWVARRLLDDGAGMVRILVGESAERFAAAEGILPVDPISLI
                     HARERLRFVHLQAETAFHPSEAFLPGGARMPSGTVGCVARDAEGRLAAATSTGGTPYR
                     PPGRVGDTPLPGCGFYADRSAAVSTTGWGEAIASSNLAGRVVCAIERGLDPEAAAREE
                     LSRMAARIANDAGECAAGGVIVLGREGSAAWAYTTPRMVRGGWVPGRDAWSSV"
     gene            7049..7843
                     /locus_tag="AC16YU_00875"
     CDS             7049..7843
                     /locus_tag="AC16YU_00875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042577 - lipid phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046839 - phospholipid dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6063803.1"
                     /translation="MPIRPASSRIPAVLAAWAFVFSATLPSAWPADARQQPDPLRFVI
                     WPVQDVPGLIAGVSYPELIGAAGFGLVTLAASRFDRPVTERTARIPDGAPIRVVREFG
                     DVRVVLPLGVVVFAGALLSGDGRFQDAAFTSLEAVVFATTVNGLVKVATGRARPYQGL
                     GPASFEPFSGRFSFPSGHSATAFALLTPWFLYYPGPATAGLLVLAGATAFSRMVTNRH
                     WLSDVMVGSAIGFTTAYALSRRHMRVGGVRVTPGIAANAVRIDVRF"
     gene            complement(7851..8792)
                     /locus_tag="AC16YU_00880"
     CDS             complement(7851..8792)
                     /locus_tag="AC16YU_00880"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6063804.1"
                     /translation="MKTLLTDDPLEAARILRGGGLVAFPTETVYGLGADATDGRAALG
                     IFSAKGRPADNPLIVHVADQGSLDRVASSVPVVAAAILEAFSPGPITVVLPRAPSISP
                     VVSAGLDTVGLRIPAHQTAHRFLEACGVPVAAPSANRSGRPSPTTWDAVLRDLDDRID
                     CILRGPRARVGLESTVIDCTVEPPVVLRTGSVTVEALRLVVGDVRVAEAGYEGAARSP
                     GTRHPHDAPRARIVIVDRPGDVRPASAAGYIGIQPPEDGYGITRVVRDPASYAHELYD
                     FFRRCDALDLDTIVCQRVERTGVGQALMDRIERAAAR"
     gene            complement(8891..>9774)
                     /locus_tag="AC16YU_00885"
     CDS             complement(8891..>9774)
                     /locus_tag="AC16YU_00885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6063805.1"
                     /translation="WALCPTFDVDYTRKWRPGIVWRETVQYLLMNRRGEQAAGRLVRF
                     GGALRDMLRPGDPFREAMDRIPQELARAGAASTWFLKAGAHGPHDVGYRLGSRWLRDR
                     VARLLAAGHEIGLHPSYHAFAHGAYMERERDRLARLLGSPPEAVRMHFLRWQDPGTAR
                     LAERLGFRIDSTLGFADRPGFRHGTTMPFHPWDERAAGPSGLWEMPLAVMESALFNRC
                     GLDLGQAIERTEGLAGTVRRHGGALVALWHTTLWDEVDCPGWGDHFVRTLGAAVEAGA
                     AVPALSEALRAWAPGPA"
BASE COUNT         1432 a         3622 c         3431 g         1289 t
ORIGIN      
        1 agctgcgcct cggcgaaccg gtacaggagc aggaacgcaa ggacgatgcc gatccggggc
       61 ctccggaaaa acgacacgaa cgtcgcgccg aactccctga gcaccgaggc cgcggaggcc
      121 tcggccgtgg ggcgatcctc gggcgggcgc ggcagcacga accggtgcca gaccacgaac
      181 agcgtgaaca ggcccgtgag caccatgaag gtgatcgacc acgcaagcgg gatgttgccc
      241 gatcgcgcgc ggaacgtggc gcgcgtcgtg tgatcgagct tcggatccag tcgcacggcg
      301 acgagggccg cggcgcccgc gttctccgcg cggaagctca gccgctcccc ctccacgatc
      361 cggaggctgg ggtctccgcc ctcgtgcgcc acgttcacgg ccacctcgtc gccgggtgga
      421 accgcgccgc tgaccctcag gggcacgagc accacgttac cggaccggga atggggcgac
      481 ggaaccggca cgtcctcccc gaaccagcgc tggatcgtcc gcccgagggg acccgagacg
      541 gaccgcgccc accagccggg ctcctcgcgg tcgtccccgc cgctcaccgt ggccggcgct
      601 tccgtgaatc cgtggcgcag gttccacgcg tgcgccagcg cgatgagcga atcggcgtag
      661 gccgcgtcga ccggacgggc ggcgacgcgg tactcttcgg cggacgactc gaggcgcgcc
      721 gcgccccggt ccgcggccgc gccgagggta tccgggggcg ccgcctccac gaactcgatc
      781 gacgcatccg cggtgacgga gacggccagg gggtccagcc cggtcgccgt ttccaggtac
      841 ccggcgagga tcacgaggag tccctggccc gtgatcatcg cgagccggta gaaggtcgac
      901 cggatgccca cgaaccaggt ctggtcgtgc tgcgagagtc cgagcatgta gaatccgtcg
      961 gccgcgatgt cgtgcgtggc cgacgagaag gccagaagcc acaggaacgc gagcgaatag
     1021 cggaagaagt cacccgtggg cagcgtcagg gcgacgcccg cgaggcccgc cccgatcagc
     1081 agctgcatcg tgacgatcca gaaccgcttg gtccgaagga tgtcgacgat cgggctccag
     1141 agcggcttga tgacccacgg caggtacagc cagctcgtgt acagggcgat ctcggtgttg
     1201 gagatcccga ggcgcttgta catgatgacc gatacgatca tcacgaccac gtacggcagt
     1261 ccctcggcga aatagaggga cggcacccac caccaggcac ctctcggtcg tctctcgggt
     1321 cggttcatcg gtcggcaggc tgcgtggcgg ggtgcgacag gaattccagg agggcggcca
     1381 tgacccggtc ccgggcctct cggccggcga tggtctcgaa cgggaatccg agcgtcacgg
     1441 cgacgtgatc cgaccggaag gcgacggcgg cgctcatgtt ggtttcgccg tagcgcagga
     1501 tcgtccggcc gcggtcgtct gcgggctcca cggcgtcggg cgcctccgcc tcgtacgacc
     1561 cgggattccg ctcgctgtgg aaggagaagg gaccgggatc cggcaccgcg ccgtcgcccg
     1621 caggcacgac gtcacccgtc gagacggcat tccccgtcct gggccggatg cccagcacct
     1681 cccgtgcaaa ggccgacctc gggtcggact cgccggccat gagctccgtt ccgacgtacg
     1741 atcctgaaac gagaaggccc actccggccg ccgccgctcc ctcgagcgcg tccatgagca
     1801 aggggggaaa cgcccggaac gcggataccg gcgcatggcc cggcgtagtc tcgcgctcct
     1861 cgccgagcag cacgtcgacg atcgcgtgtc ccgcgaccag ctccggctcc gcggccagcc
     1921 gctcgtcgct cacggtgtcg tacccgtatc ccgccgcgcg gatggaccgg ccgtgcacca
     1981 ggggaaagtc ccgcgtgttg ccggggaaga ccatggtctc gtgcgtaccc cagctggcgc
     2041 cgtgcccggg atcgtcgtcg tcacgccagg gcacggccgt gtcgaactcg tgctgcgcac
     2101 cggtgaacga gaggtccatg ccgagagcca cgccgtggtc cagcaggtcc aggaagcccc
     2161 ggaaaggcgg tcggtcgatg gccgccggcg gggcgacgcg gtcgaagccc gacacgatga
     2221 ggacgcgcgg gcgcccgggc acccaggcgg ccgccacctc ctcggacggg aacccctctc
     2281 ccccgtcgtt caggcccgcc acgcggaatc cgtagtcacg accggcctcg accacgatcc
     2341 gggcctccgt cgtatccacc acgcgcccgc cgtcgtatcc tccctctccg atccgcgtgt
     2401 agactatgta ccgatccggg agcgccgtcg gctcgagtgg atccgggacc gggcgccacc
     2461 ggaggatcac cacgccgtcg gacaccctgg cctcgaggtg gtccgggggc aggggctgca
     2521 cgaccggatc gggctccccg tactgcgccg ccaggaaccg cagcatcgcc ttgtagatgg
     2581 accgcgcggc gtcgaagcgg aacgcggggt ccagcgcatg ccgcatgtcg gcgaagttct
     2641 ggtgcgagag gagctcgagg agcgccgccg gcacgttcgg ccggaacgcc tcgctgtagt
     2701 cccggtccca gagaggccgg cgcgcccacg cggagtcgtg gagcgcacgc aggtccgaga
     2761 cgagctgggt ctggaggatg tcggcgaaat cacggttcgc gacccgcgac atgccgtccg
     2821 gaaatacccg ggtcgagtcg gcgccctcgc tcgagtagat gagcagcgta ccgatcgtcg
     2881 tatccgcgcc ggccactcca gcgtccgtgt ggaacgcgag cgacaggtcc accggaaccc
     2941 caaggccctc ggcgccgcgg tccttgttcg gcccgaaggg cgccccgcgg aggaagttca
     3001 cccattcccc ccgtgcgcgg tagtcgtccc cgtaatcgtc cagctcgccg gtgacgtggt
     3061 agaccagcgt atcgggcatg cccgagtact gcaggtggta gcgcgctgcc tcgagccatc
     3121 gcggtcgccc gctcgtcgtg ccgccccgct ccacgagacc cgtcccgccg ccgaagcgca
     3181 cggcgtctgc cgacacgacg cggcccgccg gccccgtggc acgcagcacg accgaggccg
     3241 aatccgcgcc ggcaccctcc aggaaccgga aggtgcccac gtacacccac gtgcgctcgg
     3301 ccatcgtctg gtccaccgag agccgggtct cgcccccggc gtgccggacg atgtactcgg
     3361 cccggtcggt tgcacgggga tcgtccccgt acgagacgtg gaccgaatac tcgcccgagg
     3421 cctcgatctc cggtatccac gaaacggaat cctgcgagcc cggggccatg tccgccacgc
     3481 gccaggtgcc ctgccggaac gggttgtgtc ccgcgccgta gggcggcgtc cccgtcgcga
     3541 atcccgcgcc gggaccccgc atccaacccc tcgtctcgcg gtagccggac cggccgtccg
     3601 atccgtcgtt gtccaccacg accaggcgtg gctgcgtatc gcgctcccgc ggcagccaga
     3661 ccgtcgcccc ggcccgctcg agcatcggaa cgaggaacgg aaggacgaag gaggccggga
     3721 gcacgtcctc gacggtcgag aagagccgcg cccgctgcca ctcccaccgg tccaggttcc
     3781 gctcgtagta ccacccgtgg ctcggccaaa gcgctacgtg gcgtccgtga agcccccggc
     3841 ccggcggcgg cggaccgtcc ggcctgcgca ccagcggccg cggctccgga cgcgtcccgg
     3901 gacggcgcga ggcgtcgggc gcgagcgacg accggtacag gttgggcacg agctcctcga
     3961 gcggcagatc gagcgcgcgc agcgcgatgt gctcgacggg cgcgtccggc gcgatgcgcg
     4021 aggcgacggc ctgccgcagg gcgcgcacgc cgtcctcgcg ccatgcggtt tcgccggccg
     4081 ctcggttcaa ctccacggtg agcgtccccg tcaccggatc gaacgcggcc tcccggacgg
     4141 cggccggcgg ccccaggcgg aacccgcagt cctccaggcg gcacgcggcg atggccgatt
     4201 cggcggccgc gcgaccggca tcggacacgc gctccacggt ccatggctcc ggaggtggct
     4261 ccatgacgac gcccgatccg ccgcacgcgg caagcaccat ggcgatcgga agggcgcccg
     4321 cgggaaaggc ccggcaggga ttacggcagg acacggacat cggggccctc ctgtccgaga
     4381 cggttcaccg cggataccac caccgcgtcg ggcggacgcg ggcccagttc cagggcgcga
     4441 cgccaggccg gcacgagctc cacggaccac tccgcgccgc gcagggtccg gaggtgccac
     4501 aggaagggct ccccgccccc tcctggagcc aggtccagga tgctccggtc gccgagtctc
     4561 cgaagccgcg cttccggcgc gcccggcgcg aggccgccca accagcccgt cgccggcacc
     4621 agggccggct cgcggtacag cgcgggggcg agcaccgtgt ccaccccggc accgggctcg
     4681 agcgcccgca tggagaaatg cacgttcccc gtggcgcccg cctgctcgcg ggtgagccgg
     4741 acctgccgga cgatctcgtc cggcccccag ctcgcgcccc cggccgggat cacgcggctc
     4801 gtgaagttgc ccggccacag gtggcgggcc gccgtattct ctccggccca ccagtcgagg
     4861 agccgtccgt agggctgtcc ctgcgaatca acggcccagt agatctgggg cgtgaagtag
     4921 tccagccagc cctcctgaag ccacagccgg gcgtcggcgt agatctggtc gtactggtcg
     4981 aacccggtga cgccctccgg gtggccgggc cgccagatcc cgaacgggct gatgcccacc
     5041 ttcacccagg gcttcacgcg cttcaccatg ccgtagaggc gctccacgaa gtcgtccacg
     5101 ttggcgcgcc gccaatcggc gagggccagc gtggtgccgg cctcctcggc catgcgcctg
     5161 ctcgtcgaat cgggaaacgg cacgggaatg ccggtcgcgt ccacgaccgg gtacgggtag
     5221 aagtagtcgt ccatgtgcac gccgtccacg tcgtagcggc ggacgacgtc ctcgaatact
     5281 gcgagcgagt gcgcgctcgc cgccgggctg cccggatcga tccagagctg ctcgccgtag
     5341 cgcacgaccc actcggggtg ccggatgctc acgtggctcg agtcgtacgt tccccgcatc
     5401 gtcggatggc ccgcccggaa agggttgaac cacgcgtgca gctccaggcc gcggcggtgg
     5461 gcctcctcga cggcgaacgc gagcacgtcg tatcccggcc cccggccctg ctctccgctc
     5521 acgtaccagg aggcaggctc gagctcggac tcgtacacgg cgtcggccat gggacggacc
     5581 tggaagatga ccgcgttcat gcgcagcgcg gccgcctggt ccagcagggc gagcagttcg
     5641 tccctctgcc gcgcgggatc gaggcctggc tcggacggcc agtcgatgtt gtccactgtc
     5701 gcgacccata tgccgcggaa ctcccgcatc gcggacgggg ccgcggccgt cgccggcgcg
     5761 gcgccctcgt ctccgggcgt ggccgctccg cgagggccgg cgcagccggc caggacgagg
     5821 agcgcaagga cgacggtgct gccgagggct cgcgcggagc gccgcgcagg acgtgatctg
     5881 gaggggtcgg atggcgcgat catggtctgg aggctctgcc cgtgcacggc caatgtaccg
     5941 gcacggggcc tccggcgcta cacggacgac caggcatccc gtcccgggac ccatcccccc
     6001 cggaccatcc ggggggtcgt gtaggcccag gcggccgaac cctcccggcc gaggacgatc
     6061 acgcctcccg cggcgcactc cccggcatcg ttcgcgatcc tcgccgccat gcgcgacagt
     6121 tcttcgcggg ccgcggcctc ggggtcgagc ccccgctcga tggcgcacac gacccggcct
     6181 gcgagattcg acgacgcgat cgcctcgccc catccggtcg tgctcacggc ggccgaccga
     6241 tcggcgtaga atccgcaccc cggcaacggg gtgtccccga cgcgcccggg cggtcggtac
     6301 ggcgtgcccc ccgtcgatgt ggcggccgcg agccggccct ccgcgtcccg cgccacgcat
     6361 cccaccgttc cggagggcat gcgggcgcca ccgggcagaa aggcctcgct ggggtggaag
     6421 gccgtctctg cctgcaggtg aacgaagcga agccgttccc gcgcgtggat gagcgagatc
     6481 gggtcgaccg gaaggatgcc ctcggccgcc gcgaaccgct cggccgactc gccgacgaga
     6541 attctcacca tcccggcccc gtcgtccaga aggcggcggg ccacccagat cggattgccc
     6601 agccggtgca ccgccgccac gctgccgaac gcgagcgact cgccgtccat gaccccggcg
     6661 tccagctcca cggttccctt gcgcgtgagc acggcgccgc accccgcgtc gaagacgccg
     6721 gcggactcca tcgccgcgac gaccagcgtg gccacgtcca cggccttgcc cccggactcg
     6781 agctccgccc gaccggcctc cacggcccgg agcagcgcct cgcgctgctc gtcgaggtag
     6841 gcatccggga tgtcccaggc cccgccgtgt gcgagcaggc aaggcccttc cgtcgggatc
     6901 ggaatccctt cggagcgcag tgtgctcggc gattcggccg gtgacatggg tggatcctgt
     6961 ggtgccggag atcgacagca ttccccggtc agccgtaata taggctcgaa gcccccgaag
     7021 gatcctcgcc cgaccgccgc cggaagccat gccgatccga ccagcgtcca gccgcatccc
     7081 ggccgtcctc gcggcatggg cgttcgtctt ctcggcgacg ctgccgtcgg cgtggccggc
     7141 cgacgcccgg cagcagcccg atccgctccg tttcgtgatc tggcccgtgc aggatgtgcc
     7201 ggggctcatc gccggcgtgt cctatcccga gctgatcgga gcggccggat tcggcttggt
     7261 gacactggcc gcgtcccgct tcgatcggcc ggtcaccgag cgcacggccc ggattccgga
     7321 cggcgcaccc atccgcgtgg tgcgggagtt cggggatgtc cgggtggtcc ttcccctggg
     7381 ggtcgtggtg tttgctggcg cgctcctttc cggcgacggc cggttccagg acgcggcctt
     7441 cacgtcgctg gaggccgtcg tgttcgcgac gaccgtgaat gggctcgtga aggtcgccac
     7501 gggacgggcg cgaccatacc aggggctggg gccggcctcg ttcgagccgt tctcgggacg
     7561 gttttccttc ccgtcgggac attcggccac ggccttcgcg cttctcacgc cctggttcct
     7621 gtactacccg gggccggcga ccgccggcct cctggtcctc gccggcgcga cggccttctc
     7681 ccggatggtc accaaccggc actggctctc cgacgtgatg gtcggctcgg ccatcggctt
     7741 tacgaccgcc tacgcccttt cgcggcggca catgcgggtg ggcggggtgc gcgtgacgcc
     7801 cggcatcgcg gccaacgcgg tccggatcga cgtgcgcttc tgagcaggac tcagcgggcc
     7861 gccgcccgct cgatccggtc catgagagcc tggcctacgc cggttcgctc gacccgctgg
     7921 cagacgatgg tgtccaggtc gagcgcgtcg cagcgacgga agaagtcgta cagctcgtgg
     7981 gcgtacgacg ccgggtcgcg caccacccgc gtaatgccgt agccgtcctc cggcggctgg
     8041 atgccgatgt agcccgccgc ggaagcgggg cggacgtcgc cggggcggtc cacgatgacg
     8101 atgcgcgccc ggggcgcatc atgggggtgg cgcgtgcccg ggctgcgggc ggcaccctcg
     8161 tacccggcct ccgcgaccct aacgtccccg accacgaggc ggagcgcctc gaccgtcacg
     8221 ctgcccgtcc ggagcacgac gggcggctcc acggtacagt cgatcacggt cgactccagg
     8281 ccgacccgcg ccctcgggcc gcgcaggatg cagtcgatcc ggtcgtccag gtcccggagc
     8341 accgcgtccc acgtggtagg gctcggacgc cccgaccggt tggccgaggg cgcggccacg
     8401 ggcaccccgc acgcctcgag aaaccggtgc gcggtctggt gagccggaat ccgcaggccg
     8461 accgtgtcca gcccggccga cacgaccggc gagatcgacg gggcgcgagg caacacgacg
     8521 gtgatcgggc ccggcgagaa cgcctccagg atcgcggcgg cgacgacagg aaccgacgag
     8581 gccacccggt cgagcgagcc ctggtcggcc acgtgcacga tcagcgggtt gtcggccgga
     8641 cggcccttcg cgctgaagat ccccagggcg gcccgcccgt cggtggcatc cgcacccagg
     8701 ccgtacacgg tctcggtcgg gaacgccacc agtcctcctc cacgcaggat gcgtgccgcc
     8761 tccagcggat cgtcggtgag cagcgtcttc acggcgtaat gcgcggattc tgccgaaggt
     8821 acgacgcgcc ggccgcacgg gggcgcgacg cgcccccgcc tggcgcggga cgcaccggca
     8881 gcccggtccg tcaagccggc ccgggcgccc aggcacggag cgcctccgag agcgcgggga
     8941 cggccgctcc cgcctcgacc gccgccccga gcgtccgcac gaaatggtcc ccccagccgg
     9001 ggcagtcgac ctcgtcccag agggtggtgt gccacagggc gacgagcgcg ccgccgtgtc
     9061 gccggacggt tccggccagg ccctccgtcc gctcgatggc ctgcccgaga tccagcccgc
     9121 accggttgaa gagcgcggat tccatgaccg cgagcggcat ttcccacagg ccggacggac
     9181 cggcggcccg ctcatcccac gggtggaacg gcatcgtggt gccgtggcgg aagcccgggc
     9241 ggtcggcgaa tccgagagtg gagtcgatcc ggaagccgag ccgctcggcc aggcgggcgg
     9301 tgcccgggtc ctgccaccga aggaagtgca tccggactgc ctccggaggg gatcccagga
     9361 gccgtgcgag ccgatcccgc tcgcgctcca tgtacgcccc gtgcgcgaac gcgtggtagc
     9421 tcggatgcag cccgatctcg tgacccgcgg cgaggagccg ggcaacccta tcccgcagcc
     9481 accgcgagcc gagccggtag ccgacgtcgt gcgggccgtg cgcccccgct ttcaggaacc
     9541 acgtcgaggc ggcccccgcc cgtgcgagct cctgcgggat gcggtccata gcttcccgga
     9601 acggatctcc gggccggagc atgtcgcgga gggcaccacc gaaccggacc aggcgtcctg
     9661 cggcctgctc gccgcgccgg ttcatgagca ggtactggac cgtctcgcgc cagacgatgc
     9721 ccggacgcca cttgcgcgtg tagtcgacgt cgaatgtggg gcacagcgcc cacg
//
LOCUS       DBNHQZ010000034         2305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_144457, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000034 DBNHQZ010000000
VERSION     DBNHQZ010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_144457"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16YU_00890"
     CDS             complement(<1..308)
                     /locus_tag="AC16YU_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063806.1"
                     /translation="MSDGKHEKSESYGKARSAFDGLSMDEQATFLVESVVTMMAEGVG
                     QAGKAVSRILDDLAEKLDDLRADEAGEKQPRPASPPKAGKKSSTARKKATGTRRPKAG
                     T"
     gene            complement(494..>2305)
                     /gene="ppdK"
                     /locus_tag="AC16YU_00895"
     CDS             complement(494..>2305)
                     /gene="ppdK"
                     /locus_tag="AC16YU_00895"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR6063807.1"
                     /translation="LVNAQGEDVVAGIRTPEHIDDMARHFPEAYRQLLEATAALEKHY
                     KNMQDIEFTVQEGKLYLLQTRGGKRTGPAAVKIAVDMVAEGLVDTKTAVRDLVEPRHI
                     DQLLHPRFSETLDYSKDVIARGLPASPGAAVGQVVFSADHAEEQKAKGIRTILVRIET
                     SPEDVGGMHAAEGILTSRGGMTSHAAVVARGWGKPCVAGCGEVVIDYRSRSFSAGSVT
                     VREHDWISINGSTGEVVLGEQPLVEPRLTGDFSKFMAWADEYRTLGVRTNADTPKDAK
                     AARDFGAEGIGLTRTEHMFFEGDRIEKMREMILAESETARRTALGKLLPYQKEDFLGI
                     FRAMEGLPVTIRLLDPPLHEFLPHTEADQEVMAREMGVPIEKIRAKVQALHEFNPMLG
                     HRGCRLGITYPEITEMQTRAIIEAAVELSEEGLSVLPEIMVPLIGTVEEFRNQKALIQ
                     KTAEAVFEEKGARVEYLVGTMIEIPRAALTADSIAREAEFFSFGTNDLTQMTFGYSRD
                     DAGVFLPAYVEAKILADDPFQSIDVEGVGQLVRMGTERGRSTRPDLKVGICGEHGGDP
                     HSVHFCHEVGMNYVSCSPFRVPIARLAAAQAALAKEG"
BASE COUNT          322 a          819 c          762 g          402 t
ORIGIN      
        1 gttccggctt tcggcctgcg ggtacccgtg gctttcttcc gtgcggtgga actcttcttc
       61 ccggccttcg gaggactcgc ggggcggggc tgcttctccc cggcctcgtc cgcgcgcaga
      121 tcgtccagct tctcggcgag atcgtcgagg atgcgggaga ccgccttgcc ggcctgcccc
      181 accccctcgg ccatcatggt gacgaccgac tcgaccagga acgtggcctg ctcgtccatg
      241 gagaggccgt cgaacgccga ccgggccttg ccgtacgatt cgctcttttc gtgcttcccg
      301 tcgctcatgg cacaccgtaa agggttgtcc gggggagcct gcggaccggg aagaccggcc
      361 cgggcaatgt cccctgcagt agacggctgg gcgggcggca ggtttcaggt gccgggcgcg
      421 ccggaaacga accggtgcga ccccccgggg gagaccgcac cgtgaacgtc ggatcctgca
      481 gcccgacgtg tcgtcatccc tccttcgcca gagcggcctg cgccgccgcg aggcgggcga
      541 tcgggacccg gaagggcgag cacgagacgt agttcatacc cacctcgtgg cagaaatgca
      601 ccgagtgcgg gtcaccgccg tgctcaccgc agatgccgac tttcagatcg ggtcgcgtcg
      661 agcggccccg ctccgttccc atccgcacga gctgtccgac gccctcgacg tcgatcgact
      721 ggaacggatc gtccgcgagg atcttggcct ccacgtaggc gggcaggaag acgccggcgt
      781 cgtcccgcga atagccgaag gtcatctggg tcaggtcgtt ggtcccgaac gagaagaact
      841 cggcctcccg tgcgatgctg tccgcggtga gggcggctct cggaatctcg atcatcgtcc
      901 cgacgaggta ctccacccgg gcgcccttct cctcgaagac cgcctcggcc gtcttctgga
      961 tgagcgcctt ctggttccgg aactcctcga cggtgccgat gaggggaacc atgatctcgg
     1021 gcaggaccga caggccctcc tcgctcagct cgacggccgc ctcgatgatg gcgcgcgtct
     1081 gcatctcggt gatctccggg tacgtgatgc ccaggcggca tccccggtgg ccgagcatgg
     1141 ggttgaactc gtgcagcgcc tgcaccttgg cccggatctt ttcgatcggc acgcccatct
     1201 cgcgggccat cacctcctgg tccgcctccg tgtgcggcag gaactcgtgc agcggggggt
     1261 ccaggaggcg gatcgtcacc gggagcccct ccatggcccg gaagatgccc aggaagtcct
     1321 ccttctggta cgggagcagc ttgcccaggg ccgtgcggcg ggcggtctcc gactcggcga
     1381 ggatcatctc gcgcatcttc tcgatgcgat cgccctcgaa gaacatgtgc tccgtgcgcg
     1441 tgaggccgat gccctcggcg ccgaaatcgc gggcggcctt cgcgtccttc ggcgtgtcgg
     1501 cgttcgtccg gaccccgagc gtgcggtact cgtccgccca cgccatgaac ttactgaaat
     1561 cgccggtgag cctgggctcc acgagcggct gctccccgag cacgacctcg cccgtcgatc
     1621 cgttgatcga gatccagtcg tgctcccgga ccgtcacgga gccggccgag aaggaacggc
     1681 tcctgtagtc gatgacgacc tcgccgcacc ccgcgacgca gggcttgccc cagccgcgcg
     1741 ccaccacggc ggcgtggctg gtcatgccgc cgcgcgaggt caggatgccc tcggccgcat
     1801 gcatgccgcc gacgtcctcc gggctggtct cgatgcggac caggatcgtg cgaatgccct
     1861 tcgccttctg ctcctccgcg tggtcggcgc tgaacacgac ctgcccgaca gccgcgccag
     1921 gcgaggccgg aagcccgcgg gcgatcacgt ccttcgagta gtcgagcgtc tccgagaagc
     1981 gcgggtgcag cagctggtcg atgtggcggg gctctaccag gtcgcgcacg gcggtcttcg
     2041 tgtcgaccag gccctccgcc accatgtcca ccgcgatctt caccgcggcc gggccggtcc
     2101 gcttgccgcc gcgcgtctgc agcaggtaca gcttgccctc ctgcacggtg aactcgatgt
     2161 cctgcatgtt cttgtagtgc ttctccagcg cagcggtcgc ctcgagcagc tgccggtagg
     2221 cctccgggaa gtgccgggcc atatcgtcga tgtgctcggg cgtccggatg cccgcgacca
     2281 cgtcctcgcc ctgcgcgttc accag
//
LOCUS       DBNHQZ010000035         8815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3666994, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000035 DBNHQZ010000000
VERSION     DBNHQZ010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3666994"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16YU_00900"
     CDS             complement(<1..304)
                     /locus_tag="AC16YU_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063808.1"
                     /translation="MRRSFAAVLLLAAGSLMLPACSDLLEPEVYGDLTPETFFSSEAD
                     FNSAIVALHNAFTADWGTTDTGAGTWYASLYNADPKTYLMRSMTTTDELYSPWSTTF"
     gene            complement(381..3470)
                     /locus_tag="AC16YU_00905"
     CDS             complement(381..3470)
                     /locus_tag="AC16YU_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR6063809.1"
                     /translation="MRPLQLLLVLLILGAGSAQAQHQVSGRILDGDSGATLPGANILV
                     KGTTVGTISGSDGTYTLRAPSPNDTLQVSFVGFVTQEVPIAGRSSVDVTLQPDTAMLE
                     TVVIVGYAAQRERDVSGSITRIDASRLQPVARTSVNQMLQGQAPGLNMSTRSAQPGGG
                     VSVNIRGDISPRGSGTPLYVIDGVPITEYQSSMPGLADADLGFYGGIDRDPLAYLNPS
                     DIESITVLKDASAAAIYGSAAANGVVLITTKSGRAGALQVSYRGSYTAETPHEYFPLL
                     NATQFMQEQDRMAYERYLYENNLAPYGETDPGAVADYVPLFTEGDIAAAGEGTDWLDL
                     VTENGWMQEHNISLSGGSGNTVAYASFNYQHNDAVLRGSELDRYSARLNLDQTISSRA
                     RLRLRLNGSRLEGNNASTGSNSGGVEKYNMLQAAYAYSPTIPVYEDDGTYAYTFNRLI
                     MNPAAFLSITDESRTTNLFAAPTLELNLMENLKGTLVGQFQQESTQRGFYLPRTTNNA
                     QAPEGMAQKAEYTVQNRAFEGYLTYNREFGESTVTVVGGGGYYKAETDGTTIQGVGFF
                     TDAFLYHNIGVSSEKLMNDIQSFKSARTKVSQFGRVDYSLRDRYVLSLVARRDGSSIF
                     AEDHKYGIFPGVSAAWILTEEDFMRNLPAITQLKLRVGYGQAGNETVLSGNTLQLYSP
                     GYPFLIGTTFFNGVALSQIANPNLTWETVSTLNLGVDFGVWSNRVTGSLDAFQKTAID
                     LLDYNQLPSNNAVGRVAANIGSTRSRGVELALHAINPLGRQLRWTSDLNVAYSKATWV
                     ERNPEVALASYVDSLGAMDLVYGWETAGLVRTEADRPEYMPDARLGNMIYVDQNGDGE
                     LDEFDVVVLGNSTPRWRIGFNNTFSWKGVDLNVFVYGAFGYKRGNTYAPSSFNISQST
                     NPENTTVYAQDIWSTQNTGGTFPGIATNPYNVNNPAGNDFNLKSASFLRLRNISLGYT
                     LPTSLFRSIGTVRRARVFVNLEDLGVITSYPGFDPEYTEANPYPKSYNTTIGIELDF"
     gene            3867..5651
                     /locus_tag="AC16YU_00910"
     CDS             3867..5651
                     /locus_tag="AC16YU_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HIR6063810.1"
                     /translation="MRTGTWILALLAAAAWPVRAQEFHFGNDLSYVNQMEDCGAVFRE
                     AGEPKDVYGIFADRGTTLVRVRLWVDPSWWQVPLSQPAGVKPMYGDLVDVRETVRRAK
                     DAGMQVLLDFQYSDFWADPGRQLIPKAWVWAANDLEAMKGLVYQYTKDVLTTLDAEGL
                     MPEMVQVGNEITNGLLKHIPPASGWDPAGTVSGGWSRYAQLLNEAIRAVREVGAAASV
                     NPKIAIHLAGGLSGYLWRFWNDILGSGVTDFDIMGISYYYAWHGGDLAELEYEVADMA
                     TTFPDYEVMVLETAYPWTTQDFDSLPNIVTSTDPDYHPVSPGKQLEYLTDYTRAVMRA
                     GGSGVVFWEPAWVSTPCRTPWGRGSSHDHLVFFDPIGNDFIEAGGGRWMERTFYENLD
                     APKVLFKVDMSGQDVSGGVFISGDFGIGPGQVAPMARLVNDVWYHYTYLPAGTVGTFH
                     FRSGPEGQDRETVPSACTGDGGVDRAFTVSATGGEYAFVWGGCATNVGLGGSAPELPG
                     GIVLSPAYPNPFRSATTLSFRLPRAGRVSLAVFDLTGRRVRTLVDGWKPAGTHEITFA
                     AEGLPAGPYLCRVEGRDAGSAAVVVVRR"
     gene            5776..5862
                     /locus_tag="AC16YU_00915"
     tRNA            5776..5862
                     /locus_tag="AC16YU_00915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5810..5812,aa:Ser,seq:tga)
     gene            6314..7669
                     /locus_tag="AC16YU_00920"
     CDS             6314..7669
                     /locus_tag="AC16YU_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6063811.1"
                     /translation="MRFRIPALLILVVALSSSSASAQRVITFDEAVQIALRQSVTLRR
                     AENTVRTQRATLSQEKSSLLPSLNASSSTSRNWGLTFDQTTLQLVTQSSDRFSMSANA
                     QLNLFNGFADVAGIEQAKLNLAAEEESFTRTQQSVVFNVISSYLQVILDREQIGIRQE
                     DLAAQQRQLASIEEFVRVGARAVSDLYQQQAVVAQSEATLLTAERNLQISETRLIQVL
                     QLDPLGAYTFEAPSSDEIPLLPRQYAADDLLRSAFERRRDLRAQELAIDASAQSIRIS
                     KAGYLPRLNLTGGISTGYSSLSVDPFTRESKSFGSQLEDNRSESVGLSLSIPIFDRFV
                     TKNSVERARVQYDNARLDLENLRQTIALEVRQAYLDYETAVKRLDVTEKQVRASAQAL
                     EVEEERYAVGASTLVELTQARARYVDSASQRVQAIFQFHFQHKVIEYYQGIIDPSETL
                     F"
     gene            7700..>8815
                     /locus_tag="AC16YU_00925"
     CDS             7700..>8815
                     /locus_tag="AC16YU_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6063812.1"
                     /translation="MAKKQGSATRRLIVILGILVVVAVIVIALVASSDMFGGERAAAV
                     EVAEVQLRSVTQVVVASGKIQPEVEVIISPDVSGEIVALAVAEGDQVRQGQLLARIRP
                     DFYEAQVEQADASVLQAKANEAQRRADLLNARLEAERQKTLFERNAVSEADYQRAQTT
                     FETATAGLEAAEYAVQAAQARLRESREQLAKTTIFAPMDGTVSKLDVELGERVVGTTQ
                     MTGTEMMRIARLDQMEMEVDVNENDVVNVSLGDSAAVEIDAYPGRVFRGTVTEIANSA
                     RTTGQGTQEQVTNFPVKIRILDPHNVLAGSDGAGEVQTTAEEVPGTANPTPSFRPGMS
                     GTVDVFTQTVFDAVAIPIQAVTVRDVNALAREAREAER"
BASE COUNT         1422 a         2902 c         2987 g         1504 t
ORIGIN      
        1 cgaacgtcgt gctccacggg ctgtagagct cgtccgtcgt ggtcatggac cgcatcaggt
       61 acgtcttggg gtccgcgttg tagagggagg cgtaccaggt gccggccccc gtgtcggtcg
      121 ttccccagtc agccgtgaac gcgttgtgga gcgcgacgat cgcgctgttg aagtcggcct
      181 ccgacgagaa gaaggtctcg ggagtcaggt cgccgtacac ctcgggctcc aacaggtccg
      241 aacacgcggg cagcatgagg ctgccggcgg caaggagcag gacggctgcg aatgagcgtc
      301 tcatggtttg tctcgaatgt cagctggaat ggatttcgga cgaggggccc ggttcgggcc
      361 ccgtgtgatc cggcgaggcg tcagaagtcc agctcgatcc ctatggtcgt gttgtaggac
      421 ttcggatacg ggttggcctc ggtgtactcg gggtcgaagc ccgggtagct cgtgatcaca
      481 cccaggtcct ccaggttcac gaagacgcgg gcgcgccgga ccgtgccgat gctccggaag
      541 agagacgtcg gcagggtgta gcccagcgag atgttccgca gcctcaggaa gctggcgctc
      601 ttcaggttga agtcgttgcc ggccgggttg ttgacgttgt acgggttggt cgcgattccg
      661 gggaaggtgc cgcccgtgtt ctgcgtcgac cagatgtcct gcgcgtagac ggtcgtgttc
      721 tccgggttcg tggactggga gatgttgaag gagctcggcg cgtacgtatt gccccgcttg
      781 tatccgaagg ccccgtacac gaacacgttc aggtcgaccc ccttccagga gaaggtgttg
      841 ttgaagccga tccgccagcg cggcgtgctg tttccgagca cgaccacgtc gaactcgtcc
      901 agctccccgt ctccgttctg gtcgacgtag atcatgttgc cgagcctcgc gtcgggcatg
      961 tactcgggcc ggtcggcctc cgtcctgacg agcccggcgg tctcccagcc gtagaccagg
     1021 tccatcgcgc cgagcgagtc gacgtacgag gccagggcca cctcggggtt gcgttcgacc
     1081 caggtggcct tcgagtaggc gacgttcagg tcggaggtcc accggagctg ccggccgagc
     1141 ggattgatcg catgcagcgc gagctcgacg ccgcggctgc gggtcgaccc gatgttggcc
     1201 gccacccgtc ccacggcgtt gttcgacggc aactggttat agtcgagcag gtcgatcgcc
     1261 gtcttctgga acgcgtcgag cgatccggtg acccggttgc tccagacacc gaaatcgacg
     1321 cccaggttca gggtggatac cgtctcccat gtcaggttcg ggttcgcgat ctgcgacagc
     1381 gccacgccgt tgaagaacgt ggtcccgatc aggaacgggt agcccgggct gtagagctgc
     1441 aacgtgtttc cgctgagcac cgtctcgttt cccgcctgcc cgtagccgac gcgcagcttc
     1501 agctgggtga tggccgggag gttccgcatg aagtcctcct cggtcaggat ccaggccgcc
     1561 gagactcctg ggaagatgcc gtacttgtgg tcttcggcaa agatcgacga cccgtctcgg
     1621 cgcgccacga gcgacagcac gtaacggtcg cgcagcgagt agtccacccg gccgaactgc
     1681 gagaccttgg tacgcgccga cttgaacgac tggatgtcgt tcatcagctt ctcgctcgac
     1741 actccgatgt tgtggtacag gaaggcgtcc gtaaagaatc cgacgccctg gatcgtggtc
     1801 ccgtcggtct cggccttgta gtacccaccg ccaccgacga ccgtcacggt gctttcgccg
     1861 aactcccggt tgtaggtcag gtatccctcg aaggccctgt tctggaccgt gtactcggcc
     1921 ttctgggcca tgccttcggg ggcctgcgcg ttgttcgtgg tccgcggcag gtagaaacct
     1981 cgctgcgtgg actcctgctg gaactggccg acgagcgtcc ccttcaggtt ctccatgagg
     2041 ttgagctcga gcgtcggcgc cgcgaacagg ttggtggtcc ggctctcgtc cgtgatcgag
     2101 aggaacgcgg ccgggttcat gatcaggcgg ttgaacgtgt aggcgtacgt gccgtcgtcc
     2161 tcatagaccg ggatcgtggg cgagtaggcg tacgcggcct ggagcatgtt gtacttctct
     2221 acgccgccgg aattcgaacc ggtcgaggca ttgttgccct cgagccggct cccgttcagg
     2281 cgaaggcgca gcctggcgcg ggacgagatc gtctggtcca ggttgagccg tgcgctgtag
     2341 cggtccagct ccgagcctcg gaggacggca tcgttgtgct ggtagttgaa ggacgcgtag
     2401 gccaccgtgt tgccggagcc cccgctcagc gagatgttgt gctcctgcat ccatccgttc
     2461 tcggtgacga gatcgagcca gtccgtcccc tcgccggccg ccgcgatgtc gccctccgtg
     2521 aagagcggga cgtagtccgc cacggcgccc ggatccgtct ccccgtaggg cgcgaggttg
     2581 ttctcgtaca ggtagcgctc gtaggccatc cggtcctgct cctgcatgaa ctgcgtcgcg
     2641 ttcaggagcg ggaaatactc gtgcggcgtc tccgccgtgt agctgccccg gtagctcacc
     2701 tgcagcgctc ccgcgcgccc ggacttcgtc gtgatcagga ccacgccgtt ggccgccgcg
     2761 ctgccgtaga tggccgccgc gctcgcgtcc ttgagcacgg tgatggattc gatgtccgac
     2821 gggttcaggt acgcgagagg atcgcggtcg atgcctccgt agaaccccag gtcggcgtcc
     2881 gccaggcccg gcatggacga ctggtactcg gtgatgggca ccccgtcgat cacgtacagc
     2941 ggcgtgccgc tgccgcgcgg ggagatgtcg ccgcggatgt ttaccgaaac gccgccgccc
     3001 ggctgcgcgc tgcgggtgga catgttcagg cccggcgcct ggccctgcag catctggttg
     3061 accgacgtgc gggcgacggg ctgcagccgg ctcgcgtcga tccgggtgat ggatccgctg
     3121 acgtccctct cgcgctgggc cgcgtacccg acgatgacga cggtctcgag catcgccgtg
     3181 tccggctgca gggtcacgtc gacgctggat cgcccggcga tcgggacttc ctgcgtcacg
     3241 aagccgacga acgagacctg cagggtgtcg ttcgggctgg gtgcgcgcag ggtgtaggtg
     3301 ccgtcggacc cggagatggt tccgaccgtg gttcccttga cgaggatgtt cgccccgggc
     3361 agcgtcgcgc ccgagtctcc gtcaaggatc cgtccgctca cctggtgctg ggcctgcgcc
     3421 gaaccggcgc ccaggatcag gagcacgaga agcagttgta gcggtctcat cgttgtatgg
     3481 ccgatgcgtt ggaaaggcgg tagcggaatc tgcggacggg cggccgtagt gtctgtagga
     3541 gcggatggaa gtccgcgtga gggacattct cccgcaccac ggccccagat cgccgcccgc
     3601 acccccggga cggctccccc gccctccctg acggtcatcc cgatggaggc attccgccat
     3661 ggggaaattc ccctctctgt tgtaggggat tccccctgtg cgattctgaa cccggtctcc
     3721 gggcccgcct tcagccgtcc gtagccggcc cccgtgatcc gtggagcacc gaagggtgga
     3781 tgccccgaca gcgccccgcc ccgcggccag ctaccttcgt ccctgcaccc gctcaccgtc
     3841 ccgcccggtc caccggccgg ccctccatgc gaaccggaac atggatcctc gcgctcctcg
     3901 cggccgccgc atggcccgtc cgtgcgcagg aattccattt cggcaacgac ctctcgtacg
     3961 tcaaccagat ggaggactgc ggggcggtct tccgggaggc cggcgagccg aaggacgtgt
     4021 acgggatatt cgccgaccgc ggcaccacgc tggtccgggt gcgcctctgg gtggatccct
     4081 cctggtggca ggtccccctt tcgcagccgg ccggcgtgaa gccgatgtac ggcgatctcg
     4141 tcgacgtacg ggagacggtc cggcgggcga aggacgcggg catgcaggtg ctgctcgact
     4201 tccagtactc ggacttctgg gccgatccgg ggcggcagct gatcccgaag gcgtgggtct
     4261 gggctgccaa cgatctcgag gccatgaagg gcctggtcta ccagtacacg aaggacgtgc
     4321 tgacgacgct cgacgccgag ggcctcatgc ccgagatggt gcaggtgggc aacgagatca
     4381 cgaacgggct cctgaagcac attccgccgg cgtcgggctg ggatcccgcg ggaaccgtct
     4441 ccggcgggtg gagccgttac gcgcagctcc tgaacgaggc catccgcgcg gtgcgcgagg
     4501 tcggagccgc ggcctccgtc aatccgaaga tcgccatcca cctggcgggc ggcctgtccg
     4561 gctacctgtg gcgattctgg aacgacatcc tggggtcggg ggtgaccgac ttcgacatca
     4621 tggggatttc gtattactac gcctggcacg gaggcgacct ggcggagctc gagtacgaag
     4681 tcgcggacat ggcgacgacg tttccggact acgaggtcat ggtgctcgag acggcctacc
     4741 cctggaccac gcaggacttc gacagcctgc ccaacatcgt gaccagcacg gacccggact
     4801 accatcccgt cagcccgggg aagcagctgg aatacctgac cgactacacg cgggccgtga
     4861 tgcgcgccgg gggctcgggc gtggttttct gggagccggc gtgggtgtcg accccgtgcc
     4921 gcacgccgtg gggacgcggg tcgtcccacg accacctggt cttcttcgat ccgatcggga
     4981 acgacttcat cgaggccggc ggagggcgct ggatggagcg gacgttctac gagaacctgg
     5041 atgcccccaa ggtgctcttc aaggtggaca tgagcggcca ggacgtatcc gggggggtct
     5101 tcatttccgg ggacttcgga atcggtcccg ggcaggtcgc cccgatggcc cggctggtga
     5161 acgacgtgtg gtatcactac acgtacctgc ccgcgggcac ggtgggcaca tttcacttcc
     5221 gctcgggtcc cgaagggcag gaccgcgaga ccgttccgtc ggcctgtacc ggggacggcg
     5281 gagtggatcg ggcattcacc gtgtccgcga cgggcggcga gtacgcgttc gtctggggag
     5341 gctgcgcgac gaacgtgggc ctgggcgggt ccgcccctga gcttcccggg gggatcgtcc
     5401 tgagcccggc ctacccgaat cccttccgtt cggcgaccac gctgtccttc agactgcccc
     5461 gggccggccg cgtctccctc gcagtgttcg acctcacggg ccggagggtc cggacactgg
     5521 tcgacggttg gaagcccgcc ggaacgcacg agatcacctt cgccgcggag ggactgccgg
     5581 cggggccgta cctgtgccgg gtggagggtc gggatgcggg aagcgcggcc gtggtggtgg
     5641 tcaggcgctg agaccgctcg tggtcggctc gaatcggcgc gatggcgcag gtcccgatgc
     5701 cggactgtcc gggccggccc ccggattccg ccccgccgtc gccgtcatcc gacttcggcg
     5761 taaattgcgc gccggggagg ggtggcagag cggttgaatg cactggtctt gaaaaccagc
     5821 gtgccctcgc gggtaccgtg ggttcgaatc ccaccccctc cgcacgaacg cccgcctgcg
     5881 cccgcgcagc gggcgttcgg cgtctggcgg agtcccgagc aagcttgctt gtgaggggct
     5941 tcgacaaacg ccgaatcccc accggggtga acaggcgggc gttcgtgcgg gaccctcccc
     6001 gacgggatac ccagcgagcg aagcgagcgg aatcccaacc ccgtggcccg cgcagcgggc
     6061 gttctccgtt tcgcggtgcg ggatgcgagc gaagcgagcg gaatcccaac cccggcacag
     6121 ggcactgcaa cagcccctcc acccctccgt atgccgggcc gtttgacctc ctttccggga
     6181 gctgacgccc gtgaaaagcc tgcatacagg gtgcggagat tctgtcacga tgcccgcccg
     6241 catgcaacac cctcttcccg atcgcgtgta cggcgacatt cccgtccagc gaattcccga
     6301 gtcccgatct gccatgcgat tccgaattcc cgccctcctg atcctggttg tcgccctctc
     6361 ctcgtccagc gcgtcggctc agcgggtcat cacctttgac gaggcggtcc agatcgcgct
     6421 caggcagagc gtcacgctgc ggcgcgccga gaacaccgta cggacgcaga gggcgacgct
     6481 gtcccaggaa aagtccagcc tgctcccgtc gctcaacgcg tcgagcagca cctcccggaa
     6541 ctggggcctc accttcgatc agaccaccct gcagctggtc acccagtcga gtgaccggtt
     6601 ctcgatgagc gccaacgcac agctcaacct gttcaacggt ttcgcggacg tcgcgggcat
     6661 cgagcaggcg aaactgaatc tcgcggccga ggaagagagc ttcacgagga cccagcagag
     6721 cgtggtgttc aacgtgatct cgtcctacct gcaggtgatc ctggaccggg agcagatcgg
     6781 catccggcag gaggacctcg cggcccagca gaggcaactg gccagcatcg aggaattcgt
     6841 ccgggtgggg gcgcgggcag tgtcggacct gtaccagcag caggccgtcg tcgcgcagag
     6901 tgaagcgacc ctcctgacgg cggaacggaa tctccagatc agcgagacgc ggctcatcca
     6961 ggttcttcaa ctggaccctc tcggcgcgta cacgttcgag gccccgtcgt cggacgagat
     7021 cccgcttctt ccccgccagt atgcggcaga cgacctgctc cgcagtgcct tcgaacggcg
     7081 ccgcgacctg cgggcgcagg agctggccat cgatgcctcc gcgcagtcca tccgcatttc
     7141 gaaggccggc tacctgccgc gcctgaacct gaccggcggg atcagcaccg gatactcgag
     7201 cctctcggtc gatcccttca cccgcgaatc gaagtccttc ggctcccagc tcgaggacaa
     7261 ccggtcggaa tccgtcggcc tcagcctgag cattccgatc ttcgaccggt tcgtgaccaa
     7321 gaacagtgtc gagcgcgccc gcgtccagta cgacaacgcc cggctcgacc tggagaatct
     7381 gcgtcagacc atcgcgcttg aagtgcggca ggcctacctg gactacgaga cggccgtcaa
     7441 gcggctggac gtgaccgaga agcaggttcg agcctccgcg caggccctcg aggtggaaga
     7501 ggagcgctac gcggtcgggg cgtccacgct ggtcgagctc acgcaggcac gggcccggta
     7561 cgtcgattcc gcgagccagc gagtccaggc gatcttccag ttccacttcc agcacaaggt
     7621 catcgagtac taccagggca tcatcgatcc gtccgagacg ctcttttagg accggcccgt
     7681 acctccctcc cctgaagcca tggccaagaa gcaaggcagc gccacgcgcc ggctgatcgt
     7741 aatcctcgga atcctcgtcg tagtcgcggt catcgtgatc gcgctcgtcg cctcgtcgga
     7801 catgttcggc ggcgagaggg ccgcggcagt ggaggtggcc gaggtccagc tcaggtccgt
     7861 tacgcaggtc gtggtcgcgt ccggcaagat ccagcccgag gtcgaagtca tcatcagccc
     7921 ggacgtgtca ggcgagatcg tcgccctcgc ggtggcggag ggagaccagg tccgccaggg
     7981 gcagctcctg gcgcgcatcc ggccggactt ctacgaggcc caggtggaac aggcggacgc
     8041 gagcgtcctg caggccaagg ccaacgaggc ccagcgacgc gccgacctgc tgaatgcccg
     8101 gctcgaggcc gagcgccaga agaccctctt cgagcgcaac gccgtctccg aggccgacta
     8161 ccagcgggcc cagaccacgt tcgagacggc cacggcaggt ctcgaggccg cggaatacgc
     8221 ggtccaggcc gcgcaggccc gcctccggga gtcacgcgag cagcttgcaa agaccacgat
     8281 cttcgctccg atggacggca cggtgagcaa gctcgacgtc gagctcggcg aacgcgtggt
     8341 gggcaccacg cagatgaccg gaacggagat gatgcgcatc gcgcggctgg accagatgga
     8401 gatggaggtc gacgtcaacg agaacgacgt cgtgaacgtc tccctcggag attccgcggc
     8461 ggtcgagatc gacgcgtacc ccggacgggt cttccgcggc accgtgacgg agatcgccaa
     8521 ctcggcccgg accacgggcc aggggacgca ggagcaggtc acgaatttcc cggtgaagat
     8581 caggatcctc gatccgcaca acgtgctcgc gggctcggac ggggccggcg aggtgcagac
     8641 gacggccgag gaagtgccgg gcacagccaa cccgaccccc agcttccgcc ccggaatgag
     8701 cggcaccgtg gacgtcttca cgcagacggt tttcgacgcc gtcgcgatcc cgatccaggc
     8761 cgttacagtc cgtgacgtca atgccctcgc ccgcgaggcc cgggaggccg agcgt
//
LOCUS       DBNHQZ010000036         6819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1635702, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000036 DBNHQZ010000000
VERSION     DBNHQZ010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1635702"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1979)
                     /gene="feoB"
                     /locus_tag="AC16YU_00930"
     CDS             complement(63..1979)
                     /gene="feoB"
                     /locus_tag="AC16YU_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR6063813.1"
                     /translation="MVLVGNPNVGKSVIFGALSGRYVTVSNFPGTTVEVARGALGGNG
                     SSRPLVDTPGIQSLLATSDDERVTRDILRSERDATVVLVVDTKNLLRGLMLALELAES
                     GVRFLIDLNMADEARARGIGVDAGRLSELLGVPVVSTVALRGEGIDRLRSALARATRS
                     PVRAAYAQVLEDAVDRATAHLENGVLDRRGLAVMALSDPDLAESLASGPDDREAIARI
                     RTEVEGGLPGSMPGLVHRYRLEKARSILDEVHTRAPRQDTPAERLGRFLVHPVYGWPV
                     LAAVLYGLYLAVGVLGAGVLVDLLEGRLFGEIVNPWVTVVADRFIPVPLVRDFLVGEY
                     GAITMGLSYALAIVLPIVAIFFLAFSVLEDSGYLPRLAVMLDRMFRWMGLNGKAVLPM
                     ILGLGCDTMATMTTRILDTRKERIQVTLLLALGVPCSAQLGVILGMVAVLGAAAISIW
                     TGVVAATLLTVGWLAARLIPGERSDFILELPPIRMPGLRNIALKTVARVEWYLREVVP
                     VFLAGTAALFVLDRLRLLQDIERAMRPLVQGWLGLPVETSGAFLVGFLRRDYGAAGLF
                     AVARDGLLDPIQITVSLVVITLFMPCFANFLMIVREHGTRTALLVTAVVFPMAFLIGG
                     LLLRVLRLFPGLVS"
     gene            2166..3131
                     /gene="rsgA"
                     /locus_tag="AC16YU_00935"
     CDS             2166..3131
                     /gene="rsgA"
                     /locus_tag="AC16YU_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6063814.1"
                     /translation="MEHGAPDAEILVAGLVVRTTGSWYDVASADDVIPCKVRGRFRLE
                     GGDETNPVVIGDRVRLRKGPDGTGLIVQLHERTTVLVRRAAGRRVGVSHVIAANVDAA
                     WVIQSVDLPRFNPGFVDRFLVMAGVHRIPAGIVINKSDLLDRRTGPPIRLWTDLYRGL
                     GYPVVLTSALTGDGVGDLREALTGRISAVAGPSGVGKSTLLNAVDPGLGLRTGEVSSR
                     TGKGTHTTTSASLHALEGGGYVADTPGLREFGLVELDPPHLSHYFTEMAPVLTACRYP
                     NCTHDHEPGCAVIEAVDHGRISQERYASYLGMLRSLRLGEKDVGR"
     gene            complement(3155..5731)
                     /gene="topA"
                     /locus_tag="AC16YU_00940"
     CDS             complement(3155..5731)
                     /gene="topA"
                     /locus_tag="AC16YU_00940"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6063815.1"
                     /translation="MTRLVVVESPTKARTIRRFLPEEYRVEASMGHVRDLPASAAQIP
                     ARYKGQGWSRLGVNVDNDFEPLYVIPPDKRKVIKTLEDALRDAEELFIATDEDREGES
                     IGWHLLEVLRPKVPVRRMVFHEITREAIHDALGHTRDMDRALIDAQETRRILDRLVGY
                     SLSPLLWKKIAPRLSAGRVQSVAVRLLVSRETERILFVPASYWDLAADLVVDGSGFEA
                     SLSQLDGVRVAGGRDFDDATGRLASRLTEGVDVVTLDEARATALADSLPQAEWTVSRV
                     ESKEARRSPYAPFITSTLQQEASRKLRLSARDTMSVAQRLYEQGFITYMRTDSTTLSG
                     EAMRASRQAVEDRYGKEYLSPKPREFASKVRNAQEAHEAIRPAGTAMKTADELRLSGV
                     ERALYDLVWKRTVASQMADARLRLVSADIEAATGTTVATFRATGRTIEFPGFFRAYVE
                     GSDDPEAALEDRDQPLPRLEQGMRPGCSKVEARGHETRPPARLTDASLIRVLEGEGIG
                     RPSTYASIIDTIVSRGYVRRVGGQLVPTFTAFATNNLLEVHFEQLVDVGFTAGMEQVL
                     DDIAAGSVEAVPYLQQFYRGEDGLAQRVEAGLDAIDARKVSEIRFAKWDPYVVRVGKY
                     GPYVETSHDGDRQVASLPEDLAPGDVTRERLEELVTAGRESDRVLGIHPEGEMPVLLC
                     RGPYGHYVQLGDDDLPGKPKRVSLPRDLAPESVDLEIAVRLLSLPRVIGSHPDTGQDI
                     VSNVGRYGPYVQHGSTFASLSKDDDVLEIALERALELLSRKEMRNKPLRTIGKHPESG
                     NPVEIFAGRYGPFVKHGRTNASLPKDVAPESVTMELALELLAARAERGPAKKGRGRKK
                     KG"
     gene            5973..>6819
                     /locus_tag="AC16YU_00945"
     CDS             5973..>6819
                     /locus_tag="AC16YU_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6063816.1"
                     /translation="METPVKTGQKVFVFGNGKADGDKSMKALLGGKGANLAEMSATGL
                     PVPPGFTISTESCAWYSAQGHVWPEGLEDEVRDGIGFVEEAMGARFGDPENPLLVSVR
                     SGAAVSMPGMMDTVLNLGLNDTVVEGLAKKTGNTRFAFDAYRRFIDMFGDVVMGVDHE
                     HFEEAIHGLKKERGVENDVDLTADDLRELANRYKAIFREHTGIMFPDEPFEQLRLSIN
                     AVFGSWDGDRAVKYRQINRVRGLIGTAVNVQAMVFGNMGTNSGTGVCFTRNPSTGEAL
                     LYGEFL"
BASE COUNT         1084 a         2318 c         2397 g         1020 t
ORIGIN      
        1 ccgtgtggca ggtcagcgaa tcgacctcga attcgtagcc gcagagtctg cacttcatgg
       61 catcaggaga cgagtccggg gaagagccgc agcacgcgca gcagcaggcc gccgatgagg
      121 aaggccatgg ggaagacgac cgccgtcacg agcagcgccg tccgggttcc gtgctcgcgc
      181 acgatcatca ggaagttcgc gaagcagggc atgaacagcg tgatgaccac gaggctcacc
      241 gtgatctgga tcgggtcgag caggccgtca cgcgcgacgg cgaagagccc tgccgcgccg
      301 tagtccctcc gcaggaatcc gacgaggaag gcgcccgacg tctcgacggg gaggcccagc
      361 cagccctgca cgagcggccg catcgcgcgc tcgatgtcct ggagcaggcg cagccggtcg
      421 agcacgaaaa gagcggccgt gccggcgagg aagacgggga cgacctcgcg caggtaccac
      481 tccacccggg ccaccgtctt gagcgcgatg ttgcgaaggc ccggcatccg gatcggcggc
      541 agctccagga tgaagtcgga ccgctcgccc ggaatgaggc gcgcggcgag ccagccgacc
      601 gtcagcagcg tggcggcgac gacgccggtc cagatcgata tggccgccgc cccgagcacg
      661 gccaccatgc ccaggatcac gccgagctgc gccgagcacg gcactccgag cgcgaggagc
      721 agcgtgacct ggatccgctc cttgcgcgta tcgaggatcc gggtcgtcat cgtggccatc
      781 gtgtcgcagc cgagcccgag gatcatgggc agcacggcct tgccgttcag gcccatccag
      841 cggaacatcc ggtcgagcat caccgcgagc cggggcaggt agcccgagtc ctcgaggacg
      901 ctgaacgcca ggaagaagat ggccacgatc ggcaggacga tcgcgagggc gtagctgagt
      961 cccatcgtga tcgcgccgta ttcgccgacg aggaagtcgc ggacgagcgg gaccgggatg
     1021 aaccggtcgg cgaccaccgt gacccacgga ttgacgatct ccccgaacag cctgccttcg
     1081 agcaggtcga cgagcacgcc ggcccccagc accccgacgg cgaggtagag tccgtagagc
     1141 acggcggcca gcacgggcca gccgtagacg gggtggacca ggaaccggcc gaggcgctcc
     1201 gccggggtat cctgccgggg cgcccgggtg tggacttcgt ccaggatgga gcgcgccttc
     1261 tcgaggcggt accggtggac gagcccgggc atcgagccgg ggaggccgcc ctccacctcg
     1321 gtccggatgc gggcgatcgc ctcgcggtca tccggtccgg aggcgaggga ctccgccagg
     1381 tcgggatcgc tgagggccat gaccgcgagc ccgcggcggt ccagaacgcc gttttccagg
     1441 tgagcggtcg cgcgatctac cgcgtcctcc aggacctgcg cgtacgccgc ccgaacgggc
     1501 gaccgggtcg cccgggcgag cgccgaacgc aggcggtcga tgccctcgcc ccgaagggcg
     1561 accgtcgaga cgacgggcac gcccaggagc tcggacaacc tgcccgcgtc cacgccgatt
     1621 ccccgcgcgc gggcctcgtc ggccatgttc aggtcgatca gaaagcgtac ccccgactcg
     1681 gcgagctcca gcgcgagcat gaggccccgg agcaggttct tggtgtcgac caccagcacg
     1741 acggtggcgt cgcgctccga tcgcaggatg tcccgcgtga cccgctcgtc gtccgatgtc
     1801 gcgagcaggc tctggattcc cggcgtgtcg acgagcggcc gggaagagcc gttgcccccg
     1861 agtgcgcccc gggccacctc gaccgtggtg ccgggaaaat tcgacacggt gacgtaccgc
     1921 ccggagagcg ccccgaagat caccgacttg cccacgttcg gattcccgac gaggaccacg
     1981 ggggattcac cggccgaatc ggttcgtgtg gcggacggct gcatcggatc tgcgggcggc
     2041 cccggtcggg tggcgcgtgc tgtacacgac cgggaacggg gtgtttccag gcggtggcgg
     2101 gttgaaacgg tcgtgccgca gcgacgttct tggatgcggt tcaattcatt ccgtccgcac
     2161 gagccgtgga gcacggggca cccgacgccg aaatcctggt cgccggcctg gtcgtgcgca
     2221 cgaccgggag ctggtacgac gtggcctcgg ccgacgacgt catcccgtgc aaggtacgcg
     2281 gccggttccg gctcgagggg ggggacgaaa ccaaccccgt cgtgatcggc gaccgggttc
     2341 ggctgcggaa ggggcccgac ggaacaggcc tcatcgtgca gctgcatgag cgcacgacgg
     2401 tcctggtccg ccgggccgcc ggccggcggg tgggcgtctc gcacgtgatc gcggcgaacg
     2461 tggacgcggc ctgggtcatc cagtcggtcg acctgccccg cttcaatccg gggttcgtgg
     2521 accgcttcct cgtgatggcc ggcgtgcacc ggatcccggc cggcatcgtg atcaacaagt
     2581 cggacctgct cgatcgaagg accggtcctc cgatccgcct ctggacggac ctgtaccggg
     2641 gcctcggata cccggtagtg ctcacgagcg ccctgacggg cgacggcgtc ggcgacctgc
     2701 gggaagcgct caccggccgg atcagcgcgg tcgccggacc gtccggcgtc gggaagtcca
     2761 cgctgctgaa cgccgtggat ccggggctcg gcctccgcac cggtgaggtg agctcgagaa
     2821 cgggcaaggg cacgcacacg acgaccagcg cctctctgca cgccctggaa ggcggcggtt
     2881 acgtggcgga cacgcctggc ctccgggagt tcgggctggt ggaactggac ccgccccacc
     2941 tctcgcatta cttcaccgag atggcgccgg tgctcaccgc atgccggtat cccaactgca
     3001 cgcacgacca tgagccgggc tgcgcggtca tcgaggccgt ggatcacggt cgcatcagcc
     3061 aggagcgcta cgccagctac ctgggcatgc tgcgctccct gcgactcggc gaaaaggacg
     3121 tcggccgctg accggccggc gccgattccc cggatcagcc cttcttcttc cggccgcgcc
     3181 ccttcttcgc ggggccgcgt tccgcccgtg cggcgaggag ttcgagggcg agctccatgg
     3241 ttaccgactc cggggccacg tccttcggca gggacgcgtt cgttcgaccg tgcttgacga
     3301 acggaccgta ccgtccagcg aagatctcga ccggattgcc gctctccggg tgctttccga
     3361 tcgtgcgcag gggcttgttg cgcatttcct tgcggctgag gagctcaagt gccctctcaa
     3421 gtgcgatctc caggacgtcg tcgtccttcg acagcgaggc gaacgtggat ccgtgctgca
     3481 cgtaaggacc gtaccggccg acgttcgata cgatgtcctg ccccgtgtcg ggatgcgacc
     3541 cgatgacgcg ggggagggag agcaggcgca cggcgatttc gaggtccacg ctctcgggcg
     3601 cgaggtcgcg tggcagggag acccgcttgg gcttccccgg aaggtcgtcg tcgccgagct
     3661 gtacgtagtg tccgtacggt ccgcggcaca gaaggacggg catctcgccc tccgggtgga
     3721 tgcccagcac gcggtcggac tcgcgcccgg cggtcacgag ctcctcgagg cgttcccggg
     3781 tcacgtcacc cggcgccagg tcttccggca gggatgccac ctgccggtcg ccgtcgtgcg
     3841 acgtctccac gtacggcccg tacttgccta cccgcaccac gtaagggtcc catttcgcga
     3901 accggatttc cgagactttc ctggcatcaa tggcatcgag gcccgcctcc acgcgctgcg
     3961 cgaggccgtc ctcgccccgg tagaactgct gcaggtacgg aacggcctcc acgctcccgg
     4021 ccgcgatgtc gtcgagcacc tgctccatgc cggcggtgaa tcccacgtcg accagctgct
     4081 cgaagtgcac ctccaggagg ttgttcgtgg cgaacgccgt gaacgtcggg acgagctggc
     4141 ccccgacgcg gcgcacgtaa ccacgggaga cgatcgtgtc gatgatgctc gcgtacgtgc
     4201 tcgggcggcc gatgccctca ccctcgagca cgcggatcag cgaggcgtcg gtgagccggg
     4261 ccggggggcg ggtctcgtgg cccctggcct ccaccttgga gcagcccgga cgcatgccct
     4321 gctcgaggcg cggcagtggc tggtcgcggt cctcgagggc cgcctcgggg tcgtccgagc
     4381 cctctacgta ggcgcggaaa aagcccggaa actcgatggt tcggccggtc gcccggaacg
     4441 tggccacagt cgtcccggtg gccgcctcga tgtcggccga caccaggcga aggcgggcgt
     4501 ccgccatctg ggacgcgacg gtccgcttcc agacgaggtc gtacagggcc cgctcgaccc
     4561 cggagagacg gagctcgtcg gcggtcttca tggccgttcc ggccggccgg atcgcctcgt
     4621 gcgcctcctg ggcgttccgc accttgctcg cgaactcgcg cggcttcggg ctcaggtact
     4681 ccttgccgta gcgatcctcc acggcctgcc ggctcgcccg catggcctcg ccggagagcg
     4741 tggtcgagtc ggttcgcatg tacgtgatga aaccctgctc gtacaggcgc tgcgcgaccg
     4801 acatcgtgtc gcgggcggaa aggcgcagct tgcggcttgc ctcctgctgc agcgtggacg
     4861 tgatgaacgg ggcgtagggg gagcggcgag cctccttcga ttcgacccgg ctcacggtcc
     4921 actcggcctg cggaagcgag tcggcgagcg ccgtcgcgcg cgcctcgtca agggtcacga
     4981 cgtcgacgcc ttccgtgagc ctcgaggcga gacgcccggt ggcgtcgtcg aaatcacgtc
     5041 ccccggccac ccggactccg tcgagctgcg agagggatgc ctcgaagccc gacccgtcca
     5101 ccaccaggtc cgcggcgagg tcccagtacg aggccgggac gaagagaatg cgctcggtct
     5161 cccgtgaaac caggaggcgg acggccacgc tctggacccg gccagccgaa agacgcggcg
     5221 cgatcttctt ccagagcagg ggcgaaagcg agtagcccac gaggcgatcc aggatgcgtc
     5281 gggtctcctg ggcgtcgatc agggcccggt ccatgtcgcg cgtgtgcccg agcgcgtcgt
     5341 ggatggcttc ccgcgtgatc tcgtggaaca ccatgcgccg cacggggacc ttcgggcgca
     5401 gcacctcgag caggtgccac ccgatggatt cgccctcgcg gtcctcgtcc gtggcgatga
     5461 agagctcctc ggcatcgcgc agggcatcct cgagagtctt gatcaccttg cgcttgtccg
     5521 gcgggatgac gtacagcggc tcgaagtcgt tgtccacgtt cactccgagg cgcgaccatc
     5581 cctgcccctt gtaccgtgcg gggatctggg cggccgacgc cgggaggtcc cggacatgcc
     5641 ccatgctggc ctccacgcgg tactcctccg gcaggaaccg gcgaatggtg cgggcctttg
     5701 tgggcgattc gacgacgacg aggcgggtca tgcgggtacg gagagggggt gccggcacgc
     5761 ggcggggtcc gtcagacgcc gaggccgccc cgccgttccg caccacggcg ggaaccggcc
     5821 ggccgcccgg tccgggcccc gggggaatcg gaagcgcttc ctttaatcat ttcttcccgc
     5881 cggccgccca cgggcacgaa accccttgtg tactttgact tgcttcgcta tatgcgacag
     5941 aatctgcaag caatacccct acgatgatgg atatggaaac tccggtcaag accggccaga
     6001 aggtcttcgt cttcggcaac ggcaaggccg acggcgacaa atcgatgaag gcccttctcg
     6061 gcggcaaggg cgccaacctg gccgagatga gcgcgacggg cctgcccgtg cctcccggct
     6121 tcacgatcag caccgagtcg tgcgcgtggt acagcgcgca ggggcacgtt tggccggagg
     6181 gcctggagga cgaggtccgg gacggcatcg ggttcgtgga ggaggcgatg ggcgcccgat
     6241 tcggcgaccc ggagaatccg ctcctcgtct cggtgcgctc gggcgcggcc gtctcgatgc
     6301 cgggcatgat ggacaccgtc ctgaacctgg gcctgaacga caccgtcgtc gaggggctgg
     6361 cgaagaagac gggcaacacg cgctttgcgt tcgatgccta ccggcgcttc atcgacatgt
     6421 tcggtgacgt ggtcatgggg gtggaccacg agcacttcga ggaggccatc cacggcctga
     6481 agaaggagcg cggcgtggag aacgacgtcg acctgacggc ggacgacctg cgcgagctcg
     6541 cgaaccgcta caaggcgatc ttccgcgagc acactgggat catgttcccg gacgagccct
     6601 tcgagcagct gcgcctctcg atcaacgccg tcttcggatc gtgggacggc gaccgcgccg
     6661 tcaagtaccg ccagatcaac cgggtgcgcg gcctcatcgg cacggcggtg aacgtgcagg
     6721 ccatggtgtt cggcaacatg gggacgaact ccggcaccgg cgtgtgcttc acgcgcaatc
     6781 cgtccaccgg cgaggcgctc ctctacggcg agttcctgg
//
LOCUS       DBNHQZ010000037         3395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2177318, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000037 DBNHQZ010000000
VERSION     DBNHQZ010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2177318"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /locus_tag="AC16YU_00950"
     CDS             complement(<1..1929)
                     /locus_tag="AC16YU_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR6063817.1"
                     /translation="MPRILVDPLNLNYALVGLAALTSAGYFIRVKGKTAPTWYMLGAL
                     IALAIYILVQAFWAFTHGAIAVEAAFLLTPAGMASIAVGVLGGPFLAQAAYRFAGNPF
                     RREANAVLVVSILSVLGSTLWVFVNTSSATAISRWIVAFWSLWGVMVLIRKWRNAEGS
                     ESTARDRSGMLAVAGFLLVVVASEVLFGFNFYRGSPGSFGVFVLGQILQFIVVLAFPV
                     VYVQFAPEPTTFQVRLVGFVLLTVLSVFGVLAAKEGAMDDQRVAFDPAPAPHSLVFTP
                     AEAGFDVRSTALSFDSDIGEDLRLGDDTKAVVPLPFGLPFGGDTLRAVVVNANGAIEP
                     TDAAYPFGSFSEEFPTVADRPLIAPWYTDLNPGAGGSVHARAHPDRLVVTWSEVPEWN
                     YFRPHTVQAVLHMSGQIDFNYAVLPFDTHVGASGILVDPGSPLAGGVARLTGLAPLTG
                     IGPVANPVAIYDRRLEFKAYLHRETMPFVWVVLGTIAFVLLVAPMFFRLALTRPLDRL
                     LMGMRKVNDGDLEAELPVFVKDEFGNLAQNFNQMTQSLRTYSGEMESLVAERTRELNE
                     SLDSLKATQEQLVQQEKLASLGQLTAGIAHEIKNPLNFINNFAEVNAELADELMEAVA
                     KGEDPSAIIADLKRNAAVI"
     gene            2206..>3395
                     /locus_tag="AC16YU_00955"
     CDS             2206..>3395
                     /locus_tag="AC16YU_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR6063818.1"
                     /translation="MHSLFHSAGKGLTGSIFFPLLLASAAYAQPSGGPYGPIERTWEI
                     PTEGTVYYVAPDGDPDADGMTLATPTTIESAMARVITDDTIVLRGGVYRTGNLELNQG
                     ITLQPWADEKPVLKGTEIAEDWEALSGGVWRTSWTTLFPSEPMFWWRREREEARTPLH
                     RFNNDMVFIDGRFLQSAGSIEELDENTHYIDYEERAVYIGADPTGRTVEITAHDIAIL
                     RATTEVHGKTSDRRGFVLRGITLTQYAWTAIAIDGERHFTHLDEPVDEPVGPADPATY
                     GKQVVGTHLENVSISWCSRVAGYFRGDGLVIRHSLISDTGTEGIYVIGSSNVLLERNI
                     IQRNDIERITGYYVSAVKIINQTHDVVVRDNLVLDHPTSNGVWYDVGNRNGVFVNNYV
                     EGPGV"
BASE COUNT          650 a         1104 c         1115 g          526 t
ORIGIN      
        1 gatgacggcg gcgtttcgct tcagatcggc gatgatcgcg ctcggatcct ctcccttcgc
       61 gacggcctcc atcagctcgt ccgccagctc ggcattgacc tcggcgaagt tgttgatgaa
      121 gttgagcggg ttcttgatct cgtgcgcgat gccggcggtc aactggccca gcgaggccag
      181 cttctcctgc tgcacgagct gctcctgggt cgccttgagg gagtcgagtg actcgttgag
      241 ctctcgcgtc ctttcggcaa ccagcgattc catttcgccg gagtaggtcc ggagcgactg
      301 cgtcatctgg ttgaagttct gcgccaggtt gccgaattcg tccttgacaa agacgggcag
      361 ctcggcttcc aggtcgccat cgttgacctt gcgcattccc atgaggagcc ggtcgagcgg
      421 cctggtcagc gcgagacgga agaacatcgg agcaacgagc aggacgaacg cgatcgtccc
      481 gagcacgacc cagacgaacg gcatcgtctc acggtgcagg tacgccttga actcgaggcg
      541 tcggtcgtag atggcgacgg gattggcgac ggggccgatc cccgtgaggg gggcgaggcc
      601 ggtgaggcgg gcgaccccac ccgccagggg cgaacccggg tccaccagga tgccacttgc
      661 gccgacgtgc gtgtcgaacg gcagcaccgc gtagttgaag tcgatctgcc ccgacatgtg
      721 cagcacggcc tgcaccgtat ggggccggaa gtaattccac tccggcacct cgctccaggt
      781 gaccaccagc cgatccggat gggcgcgtgc atgcaccgat cccccggcgc cgggattcag
      841 gtccgtgtac cacggggcga tgagcggacg atcggcgacc gtcgggaact cctcgctgaa
      901 gctcccgaag gggtaggcgg cgtccgtcgg ctcgatggct ccgttggcat tcaccaccac
      961 tgcacgcagc gtatctccgc caaatgggag gccgaacgga agcggtacga cggccttcgt
     1021 gtcgtccccg aggcgcaggt cctcaccgat gtcgctatcg aaggacaggg ccgtgctcct
     1081 gacgtcgaac ccggcttcgg ccggtgtgaa caccagcgag tgcggcgcgg gcgccggatc
     1141 gaacgccacc cgctgatcgt ccatggcgcc ttccttggcc gcgagcaccc cgaacacgga
     1201 gagcacggtc agcagaacga atccgacgag cctgacctga aacgtcgtcg gctccggagc
     1261 gaactggacg tagaccacgg ggaaggccag cacgacgatg aactgcagaa tctgccccag
     1321 gacgaacacg ccaaacgacc cgggcgaccc ccgatagaag ttgaagccga aaaggacctc
     1381 cgaagcgacg acgaccagga ggaaccccgc gacggcgagc atcccggaac ggtcgcgggc
     1441 ggtactctcc gaaccttcgg cattgcgcca ctttcggatg aggaccatca cgccccagag
     1501 tgaccagaag gcgacgatcc atcgggaaat ggccgtggcc gacgacgtat tgacgaacac
     1561 ccacaacgtg gaccccagta ccgacaggat ggagacgacg aggaccgcgt tggcctctcg
     1621 tcggaaggga ttgcccgcga accgataggc ggcctgggcc agaaagggtc ctccgagaac
     1681 acccacggcg atgctcgcca ttccggcggg tgtcagaagg aacgcggctt ccaccgcgat
     1741 cgccccgtgc gtgaacgccc agaacgcctg gaccaggatg tagatggcca gcgcgatgag
     1801 cgcgccgagc atgtaccacg tcggcgccgt cttgcctttg acccgaatga aataccccgc
     1861 cgacgtgagg gccgcgaggc ccaccagcgc gtagttcaga ttgagggggt cgacgagtat
     1921 tctgggcatt tccggggggg agtcggcggg tattcgaccg agtcggttcg gctccgcggt
     1981 cgctcgcgcg ggagtatgcg gtcccggttg gggtatccgg agccggcctg tccgaacata
     2041 ccgaaaagta tctcgatgta gtccctgcaa gccggcatgt tctccaggcc tgcccccggc
     2101 ccgaagagga cgtgcttgcg gatcggagtc cggaatcgta aacaggttcg cccggaccgg
     2161 gaccggcgct cccggcaccg ctgagccctc acaaaggatc ttgccatgca ttccctcttc
     2221 cactcggccg gaaagggcct gaccggatcg atcttcttcc cgctgctgct ggcgtcggcc
     2281 gcgtacgcgc agccctcggg cggtccgtac gggccgatcg agcggacctg ggagatcccg
     2341 acggagggga ccgtctacta cgtggctccg gacggcgacc cggacgccga cggaatgacc
     2401 ctcgcgacgc cgacgaccat cgaatccgcc atggcccggg tgattacgga cgacaccatc
     2461 gtcctgcgcg gcggagtcta ccgcaccggc aacctggaat tgaaccaggg gatcaccctt
     2521 cagccctggg ccgacgagaa acccgtcctg aaaggcacgg agatcgccga ggactgggag
     2581 gcgctctcgg gcggcgtgtg gcgaacctcc tggaccacgc tcttcccgtc ggagcccatg
     2641 ttctggtggc gacgcgaaag ggaggaagcg cgcacgccgc tgcaccgctt caacaacgac
     2701 atggtgttca tcgacggccg gttcctccag tccgcgggca gcatcgagga gctggacgag
     2761 aacacgcact acatcgacta cgaggagcgg gcggtctaca tcggcgccga tccgacgggc
     2821 cgcacggtgg aaatcacggc gcacgacatc gccatcctgc gcgccacgac cgaggtgcac
     2881 ggcaagacga gtgacaggcg ggggttcgtc ctgcgcggaa tcacgctcac gcaatacgcc
     2941 tggaccgcga tcgcgatcga cggcgagcgc cacttcaccc acctggacga acccgtcgac
     3001 gagcccgtcg gcccggcgga tccggccacc tacggcaagc aggtggtcgg cacgcacctc
     3061 gagaacgtca gcatctcctg gtgcagccgc gtggccgggt attttcgtgg cgacggcctg
     3121 gtgatccgcc attccctgat ctcggacacg ggcaccgagg gcatctacgt gatcggttcc
     3181 tcgaacgtgc tgctcgagcg caacatcatc cagcggaacg acatcgagcg gattacgggc
     3241 tactacgtct ccgcggtcaa gatcatcaac cagacccacg acgtggtggt gagggacaac
     3301 ctggtgctgg accaccccac ctcgaacggg gtgtggtacg acgtgggaaa tcgaaacggg
     3361 gtattcgtca acaactacgt cgagggaccg ggcgt
//
LOCUS       DBNHQZ010000038         1771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1093703, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000038 DBNHQZ010000000
VERSION     DBNHQZ010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1093703"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16YU_00960"
     CDS             complement(<1..271)
                     /locus_tag="AC16YU_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR6063819.1"
                     /translation="MQTTLPSGAAGHEPAELPSATTWSDEDIEAAIRFASGFIVPSLD
                     LACEVHDGIAILRGHPADHDERLRALDMAADIPGVRHVIDRLASVR"
     gene            complement(406..>1771)
                     /locus_tag="AC16YU_00965"
     CDS             complement(406..>1771)
                     /locus_tag="AC16YU_00965"
                     /inference="COORDINATES: protein motif:HMM:NF040057.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HIR6063820.1"
                     /translation="FTRLTDPARRAAGDTAATSINWIDALLSQSDFERATGQEERFAF
                     RATHRVPVSAPFSVSRLPLLGDFRMNLSPSVSYTEDWFVRTERQELVTVDSVSSVETR
                     SVPGFFALRQYSASISASSVFYGIFPLRIGPYSGLRHTVRPSIGFGFRPDFQAGHWGY
                     TRTYVDAAGNVVTYPVVPSVGRGEQQSLTFSLNNTFETRRPPEDSTAANARSRTVKLL
                     DLNVASSYNLAADSLGFGAINLTGRTRLFGQVDVDFRSAFSPYRLSGTGALIDEYAFS
                     ARSPFGRLTSASLTVRTSLRSSTRAGAGRPLTTPRAGFAPEGTSPFGTIDPTLQQPFG
                     GEGTDFSIPWSLSLDLTYGITRTGLTSFRRAIVNTSFDFSLTPNWKVTGRSGYDFELG
                     RVVTTNLALARDFDCWQMAVTWVPFGEYQSWGFDLHVKSGHLRDLLRLRQPKSDVRGR
                     FGSM"
BASE COUNT          296 a          568 c          662 g          245 t
ORIGIN      
        1 tccggacgga cgcgaggcga tcgatcacgt ggcgcacgcc agggatgtcc gccgccatgt
       61 ccagcgcccg gagccgctcg tcgtggtcgg ccggatggcc gcggagaatg gcgatgccgt
      121 cgtggacctc gcacgcgaga tcgagggacg gaacgatgaa cccggaggcg aaccggatcg
      181 ccgcctcgat gtcctcgtcc gaccacgtcg tggccgaagg aagctccgcg ggctcgtgcc
      241 cggctgcgcc ggacgggaga gtagtctgca tgaggggaaa ttctggccgc gcggcaggaa
      301 gagcttgacg gcgctgcgtc cggacgcggc ctggttgagg gatcggtctg tgcgggacga
      361 gcagtgcaag tgccgggcca aagcgggagc cgcgtccgat cgccgtcaca tcgagccgaa
      421 gcggccgcgc acgtccgatt tcggctgccg gaggcggagg aggtcgcgga gatgcccgct
      481 cttcacgtgc aggtcgaacc cccacgactg gtactcgccg aagggaaccc aggtgacggc
      541 catctgccag cagtcgaagt cgcgcgccag ggccaggttc gtcgtgacca cgcggcccag
      601 ctcgaaatcg tacccggacc ggccggtgac cttccagttg ggcgtgagag agaagtcgaa
      661 gctcgtattc acgatcgcgc gccggaagga ggtgagcccg gtgcgcgtga tgccgtacgt
      721 caggtcgaga gagagcgacc acgggatgga gaagtccgtg ccctcgccgc cgaagggctg
      781 ctgcagcgtc gggtcgatcg tgccgaacgg actcgtgccc tcgggggcga aaccggcccg
      841 gggcgtcgtg agcggccgtc cggcgcccgc gcgggtactg ctgcgcagcg aggtccgcac
      901 ggtgaggctg gcgctcgtca ggcggccgaa gggtgaccgg gccgagaacg cgtactcgtc
      961 gatgagcgcc ccggtccctg agagccggta gggcgagaag gcggaacgga aatccacgtc
     1021 cacctggcca aagagcctcg tgcgccccgt caggttgatc gccccgaatc cgagcgagtc
     1081 ggccgccagg ttgtagctgc tcgcgacgtt caggtccagg agcttcaccg tgcggctccg
     1141 cgcgttcgcg gcggtcgagt cctcgggcgg ccggcgcgtc tcgaacgtgt tgttgagcga
     1201 gaaggtgagc gactgctgct cgccgcgtcc cacggacggc accacgggat aagtgaccac
     1261 gttgccggcg gcgtccacat acgtccgcgt gtagccccag tggccggcct ggaaatcggg
     1321 ccggaatccg aagccgatgc tgggacgcac cgtgtgccgg agccccgagt agggtcctat
     1381 gcgcagcggg aaaatgccgt agaacacgct gctggcggag atgctcgccg agtactggcg
     1441 aagcgcgaag aaccccggca cgctgcgcgt ctcgaccgac gacacgctgt cgaccgtgac
     1501 cagctcctgc cgctcggttc gcacgaacca gtcctcggtg taggaaacgc tcggcgacag
     1561 gttcatccgg aaatcgccca gcagcgggag gcggctgacc gagaacggcg ccgagaccgg
     1621 cacgcggtgc gtggcgcgga aggcgaagcg ctcctcctgt ccggtcgcgc gctcgaagtc
     1681 ggactgcgag agcagcgcgt cgatccagtt gatggacgtc gcggccgtgt cgccggccgc
     1741 gcggcgcgcc gggtccgtca gccgggtgaa g
//
LOCUS       DBNHQZ010000039        14366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2990274, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000039 DBNHQZ010000000
VERSION     DBNHQZ010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2990274"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1883)
                     /gene="mrdA"
                     /locus_tag="AC16YU_00970"
     CDS             complement(60..1883)
                     /gene="mrdA"
                     /locus_tag="AC16YU_00970"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6063821.1"
                     /translation="MMHGRTGDDPSIRGRIFMVVATLVIGLLLLRLVKLQILDAGLYS
                     GESRSNAVRELRVTPARGNILDRDGRLVVDNEDSYTVLLTPRYFDPERTGLLASLLSV
                     PDSVVARKLAEARAWSPFRPSPAFRSVPFDVIGRLLEHRRRLPGVTYEASQKRRYVAP
                     FRAAHVLGYVREITRSELDRRTDAGYRSGDLVGKSGVESAFERALRGTMGSAFRLVNV
                     HGQEVDAFRGGAEDLAPIGGQDVVLTIDYELQALAESLFVGKRGAVVALDPNNGEILA
                     LLSKPDFDPDLMSRPLSPEAWNYLVSSRDRPQFNRATQSLLPPGSTWKPMVALMALQE
                     GAITPRSTVTCNGGHPIGRGRFFRCMHVHGTITVETAIEKSCNTFFLEMMTRIDVNTF
                     ARYARTMGFGRRAPLDVGEEDTGVIPDSAYLNRRYPGWTIGTPMNLGIGQGDMGVTPL
                     QLAEFMGAVGNGEWLYTPHVVRALRSPDGTEVKPDLPPPVRLPFDREHVELVRGALRL
                     VMEGGTGRWIQIPGIPSAGKTGTAQNNRGQDDSVFIMFAPYDEPRIALGVMVENAGFG
                     STAAGPIASLLAERYLTKRIAPERRPLFERTLAVTSGPLPE"
     gene            2205..5297
                     /locus_tag="AC16YU_00975"
     CDS             2205..5297
                     /locus_tag="AC16YU_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S9"
                     /protein_id="HIR6063822.1"
                     /translation="MIRMPVRTVGLTALFVGLFLTAQPAWGQYFGRNKVQYDQFDFQV
                     FQTENFEFYLYPEEELAVRDASRMAERWYQRHTRTFLREFGERKPIIFYANDADFQQT
                     NAIGGSIGQGTGGVTESLKERVIMPLTGSYRETDHVLGHELVHSFQYDIALTRTDSTR
                     FALQLLPLWLVEGMAEYLSVGRDDPHTAMWMRDAAIRDDIPTIDQLSRDFRYFPYRYG
                     QALLAYVGGKYGDQAVANLFKLGGRAGLDSAFVYTLGISTDSLSREWIAAVKDAYLPL
                     VEDRTLADSAGRRVLAEDLDAGEINISPVVSPDGKYVAFLSERDVFAFNLFIADAATG
                     KVVKKLRETTSGSHFDAIRFINSSGSWSPDGRRFAFITFVQGDNEISILDWQSGRIER
                     RIAVEGVTALSNPAWSPDGLQLAFSGMDGGISDLYLLDLVTGEVRQLTNDRYADLQPA
                     WSPDGTTLAFVTDRGPGGTDFETLSYADERIGLLDVASGSIRTLRPFEGAHINPQFSP
                     DGRNLFFISDHDGFKDIYRYVLSDGSVHRITYLQTGVSGITALSPAMSVAAQNGRMMF
                     SVYGDNKYTVFSLEADETQGTPYARPDPAERSIAGILPPFRDRSAGLVGGYLNDPLTG
                     LPPFGDHDLRTYGARLQLDFVAPPSIGVSAGGPFGGGLVGGIGLFFSDMLGDHNLTVV
                     AQANGTFKDVGAQVAYLNQGHRLNYGGYAGHVPILYSYYSPWQVDPTTGNLYWEQYKQ
                     RIFIDQVQGIASYPFTTSRRLELSLGATRYGVDIEVDRYIYTDFGIQRERIQRNDLEP
                     DPEYFVSGGLAAVNDFSFFGFTSPVRGGRSRFEVAPFVGTKSFVRALADTRRYFGLGD
                     FTFAFRALHVGSYGLRQGEANAGLFYLGEYLGYANSRGFVRGYSFSSFDSFAECTPTE
                     TGQCAELTRLIGTRMGLVSAEFRIPLFGNEQFGLLNFPYLPIEIALFADGGLAWSAED
                     PPVLKWRRSTDERVPVFSTGISSRFNLFNYLVLEIFYAKPYQRPGKGAHFGFQLVPGW
                     "
     gene            complement(5354..6148)
                     /locus_tag="AC16YU_00980"
     CDS             complement(5354..6148)
                     /locus_tag="AC16YU_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR6063823.1"
                     /translation="MPLPFRVVDAFASAPFTGNPAAVFVLDTFPEDAFMQRVALEMSL
                     SESAFVVGSGGSTWDLRWFTPAAEVDLCGHATLATAHVLFESGLVPGDQVARFRTLSG
                     ELVVRRTGGGRLSMDFPAEPVTISEPPPGLEAALGAGVMAFGRNRMDYLVELSGEAAV
                     RALRPDIGALSRFDARGVIVTAAAAGEYDFVSRFFGPRVGVPEDPVTGSAHCALAPWW
                     HRRTGRRRMTAWQASARGGRVEVEVRGDRVEIVGSAVTVTEGRILV"
     gene            6147..7322
                     /locus_tag="AC16YU_00985"
     CDS             6147..7322
                     /locus_tag="AC16YU_00985"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR6063824.1"
                     /translation="MALGNGVSAEYRRLWYISAHSPRRMSFYDRIRPVLFRLDAERAH
                     RLGVGASRVSARLAPALIRNVLDWDSRSLSINVLGQTFPNPIGLAAGFDKNGTTVRFW
                     RVVGAGHVEVGSVSARRCRGNPRPRAFRLPEDEALINRMGLNNQGAGRIARRLARLPR
                     DRAPLGINIVKTNDPSVTGEEAVADFVESFRLLAPLAGYLTLNLSCPNTADGRTFEDP
                     NALDDLLTAIFAARADVAPHVPVLLKLSPPISDRVVLDSAVDEIVTVALAHGVAGFVA
                     TNTTTDREGLETDPARVEAIGSGGLSGPPLENRSTRMVRYLYRRTAGLVPIIGVGGVA
                     STEQAYRKIRAGASLVQLYTGLVYHGPGLIRRMKIGLSDMLARDGFGRLADAVGVDA"
     gene            7395..9263
                     /locus_tag="AC16YU_00990"
     CDS             7395..9263
                     /locus_tag="AC16YU_00990"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6063825.1"
                     /translation="MPAVVPFETTPQLFRNLSGHFGGKRRTAVSYMDRRTREWTPIPW
                     EELTLMVESMAGYLYGEGIRPGDRVAILSENRPEWAIADLATQLLGAVNVSLYTSLPS
                     SQVAYILKDSGSAVLVTSTGIQLKKAEEIFDECPDLRRVVTMSELRKDHPGYVQSWDQ
                     AIEAGLAVARESGVEIRALERAVRPDDLSALIYTSGTTGYPKGVMLTHGNFCSNVTAA
                     LERVPFGPDDHHLSFLPLCHSFERTAGYLAVLACGARITYAQSIDTVSRDLPEVRPTV
                     LISVPRLFERVYNVILRSVEEGPSFRKRIFQWAVGAGERYARAARNGRTGPVLRAERK
                     LAGHLVFNKLHEKLGGNLRFAVSGGAALPRAVGEFFMAAGVTIIEGYGLTETSPVLTV
                     NPVDHPRYGTVGHVLPGVTIGIQDVQDGGIRGQLRGEDYPSELSTVEGEIVARGPNIM
                     RGYWNNETTTRESIDEDGWYHTGDVGRFDRGYLVITDRIKHMIVSKGGKNIYPGPIEE
                     LFKTETVIDQVMVVGEGREFLTALVVVSEDAVRDLSAGHEGDPLDSPALKEALDRIFR
                     AYSKGAASHEKIRDFRIVREPFTVDNGMMTPTLKLRRRAIETQYADLIESMYADVV"
     gene            complement(9292..10536)
                     /locus_tag="AC16YU_00995"
     CDS             complement(9292..10536)
                     /locus_tag="AC16YU_00995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6063826.1"
                     /translation="MSDDRTDVLVVGGGAVGLSAAWYLLRAGCEVTIVSRDRIGVGAS
                     AGNAGMIVPSHVIPLSAPGVIAQGLRWLANPESPFHVRMRPDLDLARWIWRFRAHCTP
                     AHARYAAPILRDLSMTSVGLFPGLQDDIGDFGWRQTGLVMLYRTEKYRAENLRAADTA
                     EGLGLRVRRLDGRGVLGVEPDLRSDADGGVLYEDDGRIDPEAFLARLAVELERRGAEI
                     REGVNVRSVGKRAGGTTTVHTDRGDLSASTVVLATGAWGGRTGPLRLPVQPAKGYSVT
                     VEAVGEGPGIPMILSEEKVTITPLPGRIRFGGTLSLSGFDGSVDPRRVRPIRAQVVRY
                     RPDLSPADVDGLAVWSGFRPASPDGLPIVGRLRPRTDVVVATGHGMMGMTLAPVTGRI
                     VADLVTDERSAIDPAPLSPYRF"
     gene            complement(10533..11534)
                     /locus_tag="AC16YU_01000"
     CDS             complement(10533..11534)
                     /locus_tag="AC16YU_01000"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HIR6063827.1"
                     /translation="MTRHTFSAIDAHTAGNPVRVVIGGAPMLSGGSMLDRRLHFLAEY
                     DWIRTGLMFEPKGHDQMSGSILYPPTRADCDVAILFIETSGCLPMCGHGTIGTVTVIL
                     ENGLVTPAEPGVLRLDTPAGRVDARYEMDGERVTSVRFTNVPSFLAAEGLEVECPDLG
                     TLRVDVAYGGNFYAIVDPQERFRDLADFAVADLVRWSPVVRRRLNERYTFEHPERPVI
                     CGLSHVLWTGAPRHPEAHARNAVFYGEKAIDRSPCGTGTSSRMAQWAARGTLSVGDDF
                     VHESVIGSLFRGRVEAVTRVGDHDAIVPSIEGSAFVTGFNTLFIDDRDPYARGFQVL"
     gene            complement(11543..13120)
                     /locus_tag="AC16YU_01005"
     CDS             complement(11543..13120)
                     /locus_tag="AC16YU_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HIR6063828.1"
                     /translation="MNIIAGRRSGAGPATIAAHDPRTGERLMPDFHEATGREIDEAAR
                     AAEAALPALAAAGREGRSRLLEGIAGAIESVGDELVQRVMRETGLPEARIIGERGRTT
                     GQLRMFADLVREGSWVDARIDQARPDRLPVPKPDLRRMLTALGPVAVFGASNFPLAFS
                     VAGGDTASALAAGCPVVCKAHPGHPGTSELVAEAVAWALEEEGFPAGTFSLVHGAAPA
                     VSISLVTHPAMKAVGFTGSLRAGRALFDAATARPDPIPVFAEMGSVNPVFVLPGAARE
                     RGESIAAGLVASVTLGSGQFCTNPGLVFGIAGPDLEAFAGHVRDRVAATAPQILLHRG
                     IRDGFEAGIGRWTGTDGVRMLGSGGEPEATAAVTARPVVFVTDARTFAGRPHLHEEVF
                     GPVSLLVEAGSADELHAAASALEGNLTATIHGTERDLEEFAGLVDLLSRKVGRLIVDG
                     FPTGVEVCPSMHHGGPWPATTDARSTSVGTAAIFRFARPICYQNFPESALPPELRSAN
                     PAGIWRTVDGHLTRDAL"
     gene            complement(13164..13811)
                     /locus_tag="AC16YU_01010"
     CDS             complement(13164..13811)
                     /locus_tag="AC16YU_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6063829.1"
                     /translation="MTSADPTLADQAYAAVEEALVTLRLAPGSVLSEAVLAESIGIGR
                     TPLREALQRLATARLIQVLPRRGMIVADINLVDFLALLETRRVLDRLVVGGAARRATS
                     AQRSDIRSAAEAMERASASGDVEAFLRADRMADLALEAAAGNPYAARAAAPLHTHCRR
                     FWVRYREDGDVGRSAQLHGLMLEAVVQGDEAAATRASDALVGYLEQLTRSLLGKA"
     gene            complement(13808..>14366)
                     /locus_tag="AC16YU_01015"
     CDS             complement(13808..>14366)
                     /locus_tag="AC16YU_01015"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6063830.1"
                     /translation="RRETLAYLRTVGAASPRPIMIYNNPVAYRTDVTPDMFEELADDP
                     TFEAIKESSDDVRRITAIKNLTGDRFRIFCGVDNIALEAMVLGAVGWVAGLVCAFPEE
                     TVALHRLVRQGRLDEAVAIYRWFMPLLDLDVSTKLVQNIKLAEALTGLGTETVRPPRL
                     PLAGEERSRVEATIRRAMEARPTLP"
BASE COUNT         2214 a         5173 c         4814 g         2165 t
ORIGIN      
        1 agggccgccc acgtgagaag gctcaggtag tccagcgtcc ggtaccacgt gcgcacggct
       61 tactcgggca aagggccgct ggtcacggcc agcgtccgtt cgaagagggg tctgcgctcc
      121 ggagcgatgc gcttggtcag gtagcgctcc gcgagcaggc tcgcgatcgg tcccgcggcc
      181 gtcgatccga acccggcatt ctcgaccatc acgccgaggg cgatccgcgg ctcgtcgtac
      241 ggcgcgaaca tgatgaagac cgaatcgtcc tggccgcggt tgttctgggc cgttcccgtc
      301 ttgccggcgg acggaatccc ggggatctgg atccagcggc ccgtaccccc ttccatcacc
      361 aggcgcaggg cgccccgcac gagctcgacg tgctcccggt cgaacggaag ccggaccggc
      421 ggcgggaggt cgggcttcac ctctgtgccg tccgggctgc ggagcgcacg gaccacgtgc
      481 ggcgtgtaca gccattcccc gttgccgacc gctcccatga actccgcgag ctgaagcggg
      541 gtcacgccca tgtcgccctg gccgatgccc aggttcatcg gcgtcccgat ggtccaccct
      601 ggataccgcc ggttcagata cgcggagtcc gggatgaccc ccgtgtcttc ctcgccgaca
      661 tccagcgggg cgcgccgccc gaatcccatc gtccgggcat accgcgcgaa cgtgttgacg
      721 tcgatccggg tcatcatctc gaggaagaac gtattgcacg acttctcgat ggcggtctcg
      781 acggtgatcg tgccgtggac gtgcatgcag cggaagaagc gcccacggcc gatcgggtgc
      841 ccgccattgc aggtgaccgt gctccgcggg gtgatcgcgc cctcctgcag cgccatgagc
      901 gcgaccatcg gcttccacgt ggaccccggg ggaagcaggc tctgcgtcgc ccggttgaac
      961 tgcggcctgt cgcggctcga gaccaggtag ttccaggcct cggggctcag gggccgcgac
     1021 atcaggtccg ggtcgaaatc aggtttggaa agcagcgcga gaatctcccc gttgttcgga
     1081 tcgagagcta cgaccgcacc ccgcttgccc acgaacagcg actccgccag ggcctgcagc
     1141 tcgtaatcga tcgtaaggac cacgtcctgg cccccgatcg gcgcgaggtc ctccgcaccg
     1201 ccgcggaacg cgtccacctc ctggccgtgc acgttgacca ggcggaatgc gctccccatc
     1261 gttccgcgga gcgcccgctc gaacgcactc tcgacgccgc tcttgcccac gaggtctccg
     1321 gaccggtagc cggcgtccgt ccgtcggtcg agctccgagc gcgtgatttc ccgtacgtag
     1381 ccgagcacgt gcgccgcccg gaacggagcg acgtagcgcc gcttctgcga cgcctcgtac
     1441 gtgactcccg gcagccggcg gcggtgctcc agcaggcggc cgatcacgtc gaacgggacg
     1501 ctccggaagg ccgggctcgg cctgaacggg ctccaggcgc gcgcctcggc gagcttgcgc
     1561 gcgacgaccg agtccggaac ggacagcagg gacgcgagaa ggcccgtccg ctccgggtcg
     1621 aaatagcggg gcgtgaggag gaccgtgtac gagtcctcgt tgtcgaccac caggcgcccg
     1681 tcgcggtcca ggatgtttcc ccgggccggg gtcacgcgca gctcgcgcac cgcgttggag
     1741 cgggactcgc ccgagtacaa cccggcgtcg agaatctgca gcttgaccag ccggagcagc
     1801 agcagcccga tcacgagggt cgcgaccacc atgaagatgc ggccccggat cgagggatcg
     1861 tcgcccgttc tgccgtgcat catgcgctct ccgtacttcc ggtcatgaat ggcccggtag
     1921 ccgcccggcc ggcgggccgc gcccgggcgc gattcggcgg cgggaggggg ccgctcgcca
     1981 tcgattccgt gccggtcgac ggctcccgat tcgagatcgg acctatttcc ccgtgagttt
     2041 cgaccggcat ggcgtttctt gaataaggca accgcgtcat cgcaaacccg ttacatgctc
     2101 gttccccggc gccggatcat ccgtagggga tgccggccgg tcgacgtgta cgctacgtac
     2161 gacgccggcg cccggagcct accgccgacc attcgaaccc cgccatgatc cggatgcctg
     2221 tgcgcaccgt cggccttacc gcccttttcg tcgggttgtt cctcacggca cagcccgcgt
     2281 ggggtcagta tttcggccgc aacaaggtgc agtacgacca gttcgatttc caggtcttcc
     2341 agaccgagaa cttcgaattc tacctgtacc cggaagagga gctggccgtg cgcgacgcct
     2401 cgcggatggc ggagcgctgg taccagaggc acacccggac gtttctcagg gagttcggcg
     2461 agcgaaagcc gatcattttc tacgcgaacg acgccgactt ccagcagacg aacgccatcg
     2521 gcggatccat cgggcagggc acgggcggcg tgacggagtc gctcaaggag cgcgtgatca
     2581 tgccgctgac gggcagctac cgggagacgg atcacgtgct cgggcacgag ctcgtccatt
     2641 ccttccagta cgacatcgcg ctcacccgca cggactccac ccggttcgcc ctgcagctgc
     2701 tgccgctgtg gctggtcgag ggcatggccg agtacctgtc ggtgggacgg gacgatccgc
     2761 ataccgccat gtggatgcgc gacgccgcga tccgggacga catcccgacg atcgaccagc
     2821 tgtcgcggga cttccggtac ttcccgtacc gctacggcca ggctctcctg gcgtacgtgg
     2881 gcggcaagta cggtgaccag gcggtcgcca acctgttcaa gctgggcggg cgcgccggac
     2941 tcgactccgc gttcgtctac acgctgggca tcagtaccga ctccctgtcc agggagtgga
     3001 tcgcggccgt caaggacgca tatctgccgc tcgtcgagga tcgcacgctc gccgacagcg
     3061 ccggccgccg cgtgctcgcc gaagacctgg acgccggcga gatcaacatc tctccggtcg
     3121 tgagcccgga cggcaaatac gtcgcgttcc tctccgagcg cgacgtgttc gccttcaacc
     3181 tgttcatcgc cgacgccgcc accggcaagg tggtgaagaa gctgcgggag accacctcgg
     3241 gcagtcactt cgatgccatc cgcttcatca actcctcggg atcctggtcg ccggacggca
     3301 ggcgattcgc cttcatcacc ttcgtgcagg gcgataacga gatttcgatc ctggactggc
     3361 agtccggccg catcgagagg cgcatcgccg tggagggcgt caccgcgctc tccaacccgg
     3421 cctggtcgcc cgacgggctg cagttggcct tctccggcat ggacggcggc atcagcgacc
     3481 tgtacctgct ggacctggtg acgggcgagg tccggcagct cacgaacgac cggtatgccg
     3541 acctgcagcc ggcctggtcg cccgacggga cgacgctggc cttcgtgacc gaccgcggcc
     3601 cgggcggcac cgacttcgag accctgagct acgccgacga gcggatcggc ctgctggacg
     3661 tcgcgtcggg ctccatccgc acgctccgcc cgttcgaagg cgcccacatc aatccccagt
     3721 tctcgcccga cggccgcaac ctgttcttca tctcggacca cgacggcttc aaggacatct
     3781 accggtacgt gctctccgac gggtcggtcc accgcatcac gtaccttcag acgggcgtga
     3841 gcggcatcac ggcgctgtcc cccgccatgt cggtcgcagc ccagaacggg cgcatgatgt
     3901 tctcggtgta cggggacaac aagtacaccg tgttctcgct cgaggccgat gagacgcagg
     3961 gcacgccgta cgcccggccc gacccagcgg agcggtcgat cgccggcatc ctcccgccct
     4021 tccgcgatcg gtcggccggc ctcgtcgggg gctacctgaa cgacccgctg accggcctgc
     4081 ccccgttcgg cgaccacgac ctgcgcacct acggggcccg gctccagctc gacttcgtgg
     4141 cgccgccctc gatcggcgta tcggccggcg gacccttcgg gggaggcctc gtgggcggca
     4201 tcgggctctt cttctcggac atgctcggcg atcacaacct gacggtggtc gcgcaggcca
     4261 acggcacctt caaggacgtg ggcgcccagg tggcgtacct gaaccaggga catcgcctga
     4321 actacggggg gtacgcgggc cacgtgccga tcctgtactc gtactacagt ccctggcagg
     4381 tggacccgac caccggcaac ctttactggg agcagtacaa gcagcggatc ttcatcgacc
     4441 aggtccaggg catcgcgtcc tacccgttca cgacgtcccg ccggctcgaa ctctccctcg
     4501 gcgccacccg gtacggcgtc gacatcgagg tggaccggta catctacacg gatttcggca
     4561 tccagcggga gcgcatccag cggaacgacc tcgaaccgga tccggagtat ttcgtctcgg
     4621 gagggctcgc cgcggtgaac gacttctcgt tcttcggctt cacctccccc gtgcggggcg
     4681 gccgatcccg tttcgaggtg gctcccttcg tcgggacgaa gtccttcgtc cgggcgctcg
     4741 cggacacccg ccggtacttc ggcctcggcg acttcacgtt cgcgttccgc gccctgcacg
     4801 tcggcagtta cggcctgcgg cagggcgagg cgaatgcggg cctgttctac ctcggtgagt
     4861 acctcggcta tgcgaacagc cggggattcg tccgcggcta cagcttcagc tcgttcgaca
     4921 gcttcgccga atgcacgccc accgagaccg ggcaatgcgc ggagctcacg cgcctgatcg
     4981 gcacgcgcat ggggctcgtg agcgcggagt tccggatccc gctcttcgga aacgagcagt
     5041 tcggcctgtt gaacttcccg tacctgccca tcgagatcgc gctcttcgcc gacggcggcc
     5101 tggcgtggtc ggccgaggat ccgccggtgc tcaagtggag gcgctcgacg gacgagcgcg
     5161 tcccggtctt cagcacgggc atctcgtccc ggttcaacct gttcaactac ctggtgctgg
     5221 agatcttcta cgccaagccg taccagcgcc cgggcaaggg ggcgcacttc ggcttccagc
     5281 tggttccggg gtggtaggaa ccggcgcctt cagcgcatga gcgccgattc gagggtccgg
     5341 gcccggcacc gggtcagacc aggatccgtc cctccgtaac cgtgaccgcc gacccgacta
     5401 tctccacgcg gtcgccccgg acctcgacct ccacccgtcc gccacgggcc gacgcctgcc
     5461 acgcggtcat ccgccggcgc cccgtcctcc ggtgccacca cggagccagc gcgcagtggg
     5521 ccgatcccgt gaccggatct tccggaacgc ccaccctcgg gccgaagaag cgcgacacga
     5581 agtcgtactc tccggccgcg gccgccgtca cgatgacgcc ccgggcatcg aaccgggaga
     5641 gcgcgccgat gtcgggccgc agggcccgca cggcggcctc gccggacagc tccaccaggt
     5701 agtccatccg gttgcggccg aaggccatga cgcccgcgcc cagggcagcc tcgagtccgg
     5761 gcggaggctc ggaaatggtc accggctcgg ccggaaagtc catcgaaagc cgtccgccgc
     5821 ccgtcctgcg gacgacgagt tccccggaga gcgtccggaa ccgcgcaacc tggtccccgg
     5881 gcacgaggcc gctctcgaag agcacgtgcg cggtcgcgag cgtggcatga ccgcacaggt
     5941 ccacctcggc ggccggcgtg aaccagcgca ggtcccaggt cgatccgccg ctcccgacga
     6001 cgaaggccga ttcggacagg ctcatctcga gcgcgacgcg ctgcatgaac gcatcctccg
     6061 gaaacgtatc cagcacgaaa acggcggcgg gattgccggt gaagggggcg gacgcgaagg
     6121 cgtccacgac gcggaacgga aggggcatgg cgctcggaaa cggggtgtcg gccgaatacc
     6181 gtcggctgtg gtacatttcg gcgcacagcc cgcgccgcat gtccttctac gaccggatcc
     6241 gccccgtcct gttccggctc gacgccgagc gcgcccatcg cctcggcgtc ggagctagtc
     6301 gggtatcggc gcgcctggcg ccggccctca tccgcaacgt cctcgactgg gactcccgca
     6361 gcctgtcgat caacgtactg ggacagacct ttccgaatcc catagggctg gccgccggat
     6421 tcgacaagaa cggtaccacc gtgcggttct ggcgcgtggt cggcgccggg cacgtggaag
     6481 tcgggtcggt cagcgcgcgc cgctgccgcg gcaacccccg tccccgcgcc ttccgccttc
     6541 cggaggacga ggcgctcatc aaccggatgg gcctgaacaa ccagggggcc gggcgcatcg
     6601 ctcgccgcct ggccaggctc ccgcgcgacc gggccccgct cggcatcaac atcgtcaaga
     6661 ccaacgaccc ctccgtcacc ggcgaggagg cggtcgctga tttcgtcgag agcttccggc
     6721 tcctggcgcc gctcgccggc tacctgacgc tgaacctgtc ctgtcccaac acggccgacg
     6781 gacggacctt cgaggatccg aacgccctcg acgacctgct gacggccatt ttcgccgcgc
     6841 gggccgacgt tgccccgcac gtcccggtcc tcctgaaact ctcccccccg atctccgacc
     6901 gcgtggtcct ggacagcgcg gtggacgaga tcgttacggt ggccctggcg cacggcgtgg
     6961 cgggtttcgt ggccacgaac accacgaccg accgcgaggg actcgaaacc gatcctgccc
     7021 gggtcgaggc gatcggctcc ggagggctca gcggcccgcc gctggagaac cggtccaccc
     7081 ggatggtccg gtacctttat cgccgcaccg ccggactcgt cccgatcatc ggcgtcggcg
     7141 gcgtggcctc gaccgagcag gcctaccgga agatccgcgc aggggcaagc ctggtacagc
     7201 tgtacacggg gctcgtttac catggacccg gcctcatccg gcgaatgaag atcggcctca
     7261 gcgacatgct ggctcgcgac ggtttcggcc gcctggccga cgccgtgggc gtggacgcct
     7321 gacgcgtccg cgttgttatt cggccgatac gggagtacta tatcgcaaac ctctggctcg
     7381 cgaagccctc cgccatgccc gcggtcgtcc cgttcgaaac cacgccacag ctcttccgca
     7441 acctgtccgg tcatttcggc ggcaagcgga ggacggccgt gtcctacatg gaccggcgga
     7501 cgcgcgagtg gaccccgatc ccgtgggagg agctcaccct gatggtggag tcgatggcgg
     7561 gctacctgta tggtgagggc atccgaccgg gcgaccgcgt tgccattctc tccgagaatc
     7621 ggcccgagtg ggcgatcgcc gacctcgcga cgcagctgct cggtgcggtg aacgtctcgc
     7681 tgtacacgtc gctgccgtcg tcgcaggtcg cctacatcct gaaagactcc ggctcggccg
     7741 tgctggtcac gtcgacggga atccagctga agaaagccga ggagatcttc gacgagtgcc
     7801 cggatctccg ccgcgtggta acgatgagcg agcttcgcaa ggaccacccc ggctacgtcc
     7861 agtcctggga ccaggcgatc gaggccggac tggccgtggc ccgggaatcc ggcgtcgaga
     7921 tcagggccct ggagcgcgcc gtccgccccg acgacctctc ggcgctcatc tacacgagcg
     7981 gaaccaccgg gtaccccaag ggggtcatgc tcacgcacgg gaatttctgc tccaacgtga
     8041 cggcggcgct cgagcgcgtg ccgttcggtc cggacgacca ccacctgtcc ttccttcccc
     8101 tctgccactc cttcgagcgc acggcgggct acctggcggt gctggcttgc ggggcacgga
     8161 tcacgtacgc gcagagcatc gacaccgtgt cgcgggacct cccggaggtc cgtccgaccg
     8221 tcctgatctc ggtcccgcgc ctcttcgagc gcgtgtacaa cgtcattctg cgctcggtcg
     8281 aggagggccc ctccttccgg aagcgcatct tccaatgggc cgtcggcgcc ggcgaacggt
     8341 acgcccgggc cgcccggaac ggccgaaccg gacccgtcct gcgggccgag aggaagctgg
     8401 ccggccacct ggtgttcaac aagctgcacg agaagctggg cggaaacctg cggttcgccg
     8461 tctcgggggg cgccgccctg ccgcgcgcgg tcggggagtt cttcatggcc gccggcgtca
     8521 ccatcatcga ggggtacggc ctcaccgaga cgtctcccgt ccttacggtc aacccggtcg
     8581 accacccgcg ctacgggacg gtcgggcacg tgctgccggg cgtcacgatc ggcatccagg
     8641 acgtgcagga cggcggcatc agggggcagc tccggggaga ggattacccg agcgagctct
     8701 ccacggtcga gggcgagatc gtcgcccggg gccccaacat catgcgcggg tactggaaca
     8761 acgagacgac cacccgcgag tcgatcgacg aggacgggtg gtaccacacg ggcgacgtcg
     8821 ggcggttcga ccggggatac ctggtcatca cggaccgcat caagcacatg atcgtctcga
     8881 agggcggcaa gaacatctac ccgggaccga tcgaggagct gttcaagacc gagaccgtca
     8941 tcgaccaggt catggtggtc ggcgaagggc gcgaattcct caccgcgctc gttgtggtct
     9001 cggaggatgc cgtccgggac ctgtcggccg ggcacgaggg cgacccgctg gactccccgg
     9061 cactgaagga ggccctggac cggatcttcc gggcgtactc gaaaggggcg gcgtcccacg
     9121 agaagatccg ggacttccgc atcgtccggg agccgttcac ggtcgacaac gggatgatga
     9181 ccccgacgct gaagctccgt cgacgggcca tcgagacgca gtacgcggac ctgatcgagt
     9241 ccatgtacgc cgacgtggtg tgaggcgccc ggtccgtgcg cccggcgggc gtcagaaccg
     9301 gtagggggac aggggagcgg gatcgatggc gctccgctcg tcggtgacca aatccgcgac
     9361 gatccgtccc gtgacggggg ccagggtcat tcccatcatg ccgtgcccgg tcgcgaccac
     9421 gacgtccgtg cgggggcgca ggcgtcccac gatcgggagc ccgtccggcg aagcggggcg
     9481 gaatcccgac cataccgcga gcccgtccac gtcggcgggc gagaggtctg ggcggtaccg
     9541 gaccacctgg gcacggatcg gtcgcaccct gcgcggatcc accgagccgt cgaagcccga
     9601 gagcgaaagc gtccccccga accggatgcg cccgggcagc ggcgtgatcg tgaccttctc
     9661 ctcggacagg atcatcggaa tccccgggcc ctccccgacg gcttccacgg tgacggagta
     9721 ccccttcgcc ggctggacgg gcagacgcag cgggccggtc cggcctcccc acgcccccgt
     9781 ggccagcacg acggtcgatg cgctcagatc gccccggtcc gtgtgcaccg tcgtcgtccc
     9841 gccggcacgc ttcccgaccg aacgcacgtt cacgccctcc cggatctccg cgccgcgccg
     9901 ctcgagctcc acggcgaggc gcgccaggaa ggcctccggg tcgatccggc cgtcgtcctc
     9961 gtacagcacc ccgccgtccg cgtccgatcg caggtcgggc tcgacgccca ggactccgcg
    10021 cccgtccagg cggcgcacgc gcaggcccag cccttcggcc gtgtcggcgg ccctcaggtt
    10081 ctcggcgcgg tacttttccg tgcggtacag catcacgagg ccggtctgac gccagccgaa
    10141 gtccccgata tcgtcctgga gcccggggaa caggccgacg ctcgtcatcg acaggtcgcg
    10201 caggatgggg gcggcgtacc gggcgtgggc cggcgtgcag tgcgcccgga accgccagat
    10261 ccaccgcgcc aggtcgaggt ccggccgcat ccggacgtgg aacgggctct ccgggttcgc
    10321 aagccagcgc aggccctggg cgatcacgcc gggtgcggac agcgggatca cgtgactcgg
    10381 aacgatcatg ccggcgttgc ccgccgacgc cccgaccccg atccggtccc tcgaaacgat
    10441 cgtcacctcg cagcccgccc gcagcaggta ccacgccgcg gagaggccga cggccccgcc
    10501 gcccacgacc aggacatcgg tgcggtcgtc gctcacagga cctggaaacc gcgcgcgtac
    10561 gggtcgcggt cgtcgatgaa cagcgtgttg aagcccgtga cgaaggcgct gccctcgatg
    10621 ctcgggacga tagcgtcatg gtctccgaca cgggtcaccg cctcgacccg gccgcggaag
    10681 aggctgccga tgacgctctc gtgcacgaag tcgtccccca ccgatagcgt cccgcgggcc
    10741 gcccactgcg ccatgcgcga cgacgtcccc gtgccgcacg gcgagcggtc aatcgccttc
    10801 tcgccgtaga acaccgcgtt ccgcgcgtgc gcctccgggt gcctcggcgc ccccgtccac
    10861 aggacgtggg acaggccgca gatgaccggt cgctcggggt gctcgaacgt gtaacgctcg
    10921 ttcaggcggc ggcggacgac ggggctccag cgcaccaggt cggcgaccgc gaaatccgcg
    10981 aggtcgcgga accgctcctg cggatcgacg atcgcgtaga agttgccgcc gtaggcgacg
    11041 tcgaccctga gggtgcccag atccgggcac tcgacctcca gcccctccgc ggccaggaag
    11101 ctcgggacgt tcgtgaagcg cacggacgtg acccgctcgc cgtccatctc gtagcgggcg
    11161 tcgacgcgcc cggcgggcgt gtccagacgc agcaccccgg gctccgccgg ggtgacgagg
    11221 ccgttctcca ggatcaccgt caccgtcccg atcgtgccgt ggccgcacat cggcaggcat
    11281 cccgaggtct cgatgaacag gatcgcgacg tcgcagtccg cgcgggtcgg cgggtacagg
    11341 atggaccccg acatctggtc gtgccccttc ggctcgaaca tcaggccggt tcggatccag
    11401 tcgtactccg cgaggaagtg cagcctccga tccagcatcg acccgcccga gagcatcgga
    11461 gcgccaccga tgacaacgcg aacggggttt cccgcggtgt gggcgtcgat cgccgaaaac
    11521 gtgtgtcggg tcatcggttc gctcagagcg cgtcacgggt gaggtgtccg tccaccgtcc
    11581 gccagatgcc ggccgggttc gcactgcgca gctcgggcgg aagcgcggac tccgggaagt
    11641 tctggtagca gatcggtctc gcaaagcgga atatcgcggc cgttcccacc gatgtcgagc
    11701 gcgcgtcggt cgtggcgggc cacggtcctc cgtggtgcat cgaggggcac acctcgacgc
    11761 ccgtggggaa cccgtccacg atcaaccggc ccaccttccg cgaaaggaga tcgacgagcc
    11821 ccgcgaattc ctccaggtcc cgttccgtgc cgtgtatcgt cgccgtgagg ttgccttcga
    11881 gcgcggaagc cgcggcgtgc agctcgtcgg cgctccctgc ctcgaccagc agggacaccg
    11941 ggccgaacac ttcctcgtgc aggtgcggac gcccggcgaa ggtccgcgcg tcggtcacga
    12001 acaccaccgg ccgcgccgtg accgcggcgg tcgcctccgg ctcgcccccg ctccccagca
    12061 tgcgcacccc gtcggttccc gtccaccgtc cgattccggc ctcgaatcca tcccgaatgc
    12121 cccggtgcag caggatctga ggggccgtcg ccgcgacacg gtccctgacg tgtcccgcga
    12181 aggcttccag gtccggaccc gcgatgccga acaccaggcc ggggttcgtg cagaactgcc
    12241 ccgagccgag cgtcaccgag gccaccagcc ccgccgcgat ggactcgccc cgctcccggg
    12301 cggcgccggg gagcacgaag accgggttga cgctccccat ctcggcgaac accgggatag
    12361 ggtcgggtcg ggcggtcgcc gcgtcgaaga gagcccggcc cgcgcggagg gatcccgtga
    12421 agccgacggc cttcatcgcc gggtgggtca ccagcgagat gctgaccgcc ggggcggccc
    12481 cgtgcaccag cgaaaaggtg ccggcgggaa aaccctcctc ttcgagcgcc cacgcgacgg
    12541 cttcggccac cagttcggac gttccaggat ggccggggtg ggccttgcag accaccggac
    12601 agcccgcagc cagggccgag gccgtgtccc ctcccgcgac ggagaaggcg agcgggaagt
    12661 tcgaggcccc gaagaccgcg acgggtccga gggccgtgag catgcggcgc aggtccggct
    12721 tcggtaccgg cagcctgtcg ggccgcgcct ggtcgatccg cgcatctacc caggatccct
    12781 cgcgcaccag gtcggcgaac atccggagct ggcccgtggt ccggccccgt tccccgataa
    12841 tgcgcgcctc cgggagtccc gtttcccgca tgacccgctg cacgagttcg tcaccgacgg
    12901 actcgatggc cccggcgatg ccctcgagca gtcgcgaccg accctcgcgg ccggccgccg
    12961 ccagggccgg cagggcggcc tccgcggccc gggcggcctc gtctatctcc cggccggtcg
    13021 cctcgtgaaa gtcgggcatc agtcgctcgc cggtccgcgg atcatgggcc gcgatcgtcg
    13081 ccggacccgc gccggatcgg cggcccgcga tgatattcat tccgtgcagc atgctcaggg
    13141 tgtggtctgt tggcggactt cgatcaggcc ttcccgagga gcgagcgggt cagctgttcc
    13201 aggtatccca cgagggcgtc gctggccctg gtggccgcgg cctcgtctcc ctgcaccacc
    13261 gcctccagca tcaggccgtg cagctgggcg gatcggccga cgtccccgtc ctcccggtag
    13321 cgcacccaga accggcggca gtgcgtgtgc agcggtgcgg ccgcccgcgc cgcgtacggg
    13381 tttcccgcgg cggcctccag tgccaggtcc gccatccggt cggcgcgcag gaaagcctcg
    13441 acgtcgcccg acgcggacgc cctttccatg gcctccgccg cggaccggat gtcggaccgc
    13501 tgcgcagacg tggcccgccg cgcggcgcct ccgacgacca ggcggtccag gacgcgcctc
    13561 gtctcgagca gggccaggaa atcgaccagg ttgatgtcgg ccacgatcat cccccggcgg
    13621 ggcagcacct ggatcagccg cgccgtcgcg agccgctgaa gcgcctccct gagcggggtg
    13681 cggccgatgc cgatcgactc ggcgagcacg gcctccgaaa gcaccgatcc cggtgcgagg
    13741 cggagcgtga cgagcgcttc ctcgacggcc gcgtaggcct ggtcggcgag cgtggggtcg
    13801 gcggaggtca cgggagcgtc ggccgggctt ccatcgcccg cctgatcgtg gcctccacgc
    13861 gtgaccgctc ctcccccgcg agcggcagtc gcggcggccg tacggtctcc gtgccgagcc
    13921 cggtcagcgc ctcggcgagc ttgatgttct ggaccagctt cgtggacacg tccaggtcca
    13981 ggagcggcat gaaccagcgg tagatggcaa cggcctcgtc gaggcgtccc tgccggacca
    14041 gccggtgcag ggcaacggtc tcctccggga acgcgcagac cagtcccgcc acccacccca
    14101 cggccccgag caccatggcc tcgagcgcaa tgttgtccac cccgcagaat atccggaagc
    14161 ggtccccggt caggttcttg atggccgtga tccgacgcac gtcgtcggag gattccttga
    14221 tcgcctcgaa ggtgggatcg tccgccagct cctcgaacat gtcgggcgtg acgtccgtcc
    14281 ggtaggccac cgggttgttg tagatcatga tcggcctcgg agaggccgcc cccaccgtgc
    14341 gcaggtacgc gagcgtttcc ctgcgg
//
LOCUS       DBNHQZ010000040         4015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1365327, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000040 DBNHQZ010000000
VERSION     DBNHQZ010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1365327"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..410
                     /gene="rpsI"
                     /locus_tag="AC16YU_01020"
     CDS             15..410
                     /gene="rpsI"
                     /locus_tag="AC16YU_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6063831.1"
                     /translation="MAAIAQNIAVGRRKTSVARVYLRPGSGKVVVNHRELGNYFPLEW
                     RRRSITAPFEVTNTVGTFDVVVNVRGGGPTGQAEAIRMGIARALVDNDEARRPALREA
                     GFLTRDPRMVERKKYGQPKARKRFQFSKR"
     gene            534..1370
                     /gene="rpsB"
                     /locus_tag="AC16YU_01025"
     CDS             534..1370
                     /gene="rpsB"
                     /locus_tag="AC16YU_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6063832.1"
                     /translation="MSDTPATTPQHRVELEALLKAGTHFGHLTRRWNPKMRSYIFMER
                     NGIHIIDLTQTQRMLDEAADAVARFARRGRKVLFTGTKKQARDIVKKYAEECGSPYVT
                     ERWLGGTLTNFQTIRKSIRRMEDLIKMEEDGTMDQLKKKERLMKRREREKLEKVLSGI
                     KDLARLPGALFVVDINREHIAVKEARRLNIPIIALVDTNCDPDLVDFPIPANDDAQRS
                     IELITAAISNAVSEGDKEKEVQDASGKAEAQKRAQDVAELSGKESDGEEVEVAADSES
                     DD"
     gene            1441..2271
                     /gene="tsf"
                     /locus_tag="AC16YU_01030"
     CDS             1441..2271
                     /gene="tsf"
                     /locus_tag="AC16YU_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6063833.1"
                     /translation="MAITASDVKKLRDITGVGMMDCKRALEEAGGDFEAAIDLLRKKG
                     QKVAAKRADREAKEGVIATASTGDGRTAILVEVNCETDFVARNEEFQAFASALASLVL
                     AERPADREALLALPFQGGRTVEQALTDMTGKIGEKIDVRRIAVETSTAGRIVSYIHPG
                     ARLGVLVDIDGNGNVEAAGRDVAMQVAALNPVATTRDEVPQDVQEKELEIGRETALNE
                     GKPEQIVDRIAQGKLERYFKDSVLVEQPFVKDASISVGAMLKGAGATVKGFVRFALGD
                     "
     gene            2420..3211
                     /gene="pyrH"
                     /locus_tag="AC16YU_01035"
     CDS             2420..3211
                     /gene="pyrH"
                     /locus_tag="AC16YU_01035"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR6063834.1"
                     /translation="MSDESPADRPLRYRRILLKLSGESLLGSGEYGIDQDVLRLYADE
                     IRDGIQAGAEIAIVIGGGNIFRGLRNAHDGMTRAHADYMGMLATMINAMALQDALEQV
                     GVTTRLQSSIKMEQIAEPFIRRRAMRHLEKGRVVIFGSGTGNPYFTTDTAAALRALEI
                     DADVILKGTRVDGVFTADPEHDPAATRFKSIHGNEVIARNLRVMDMTAFTLCRESSLP
                     VVVFNFNERGNFLRLLRGEAVGTLVHWDDHRTAELAEPAAPVNRS"
     gene            3214..3783
                     /gene="frr"
                     /locus_tag="AC16YU_01040"
     CDS             3214..3783
                     /gene="frr"
                     /locus_tag="AC16YU_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR6063835.1"
                     /translation="MLHESLQLVIDDSREHMEKSIEHLRAELKTIRAGRATPAMLDGV
                     RVDYYGTSTPLAQMASISAPQPDLIVVQPWDRSVLNDVEKAIQAANLGINPSNDGSFI
                     RLPVPPLSEERRHDLVKTARTRGEDAKVAIRNVRRHSKDHIKTMQKDENLPEDMRFEA
                     EEELQRLTDEYSEKVDHLLGHKEAEIMEV"
BASE COUNT          735 a         1430 c         1317 g          533 t
ORIGIN      
        1 gcgatcctca atccatggca gcaatcgcgc agaacatcgc cgtcggtcgt cggaagacct
       61 ccgtggcccg cgtgtacctc cgtccgggct cgggcaaggt ggtggtgaac caccgcgagc
      121 tgggcaacta cttcccgctc gagtggcgtc gccgctccat caccgcgccg ttcgaggtca
      181 cgaacaccgt cggcacgttc gacgtggtcg tcaacgtccg cggcggcggg ccgaccggac
      241 aggccgaggc catccggatg ggcatcgccc gcgccctcgt cgacaacgac gaagcgcgtc
      301 gccccgccct ccgcgaggcc ggcttcctca cgcgcgaccc gcgcatggtc gagcgcaaga
      361 agtacggtca gcccaaggca cgcaagcggt tccagttcag caagcgttaa gcgttcaccc
      421 ggccccaggc cggatcatcc cgcatgtgac ccgatggcgc cccggggtgg ccgatccccg
      481 atcggctcgg tggcgccagg cgacggtcgc agaggcacaa cccgacacct gacatgtccg
      541 acacccccgc gacgacgccg cagcaccgcg tcgagctcga ggccctgctc aaggcaggca
      601 cccacttcgg ccacctgacc cgccggtgga acccgaagat gcgctcctac atcttcatgg
      661 agcgcaacgg catccacatc atcgatctga cgcagaccca gcgcatgctg gacgaggccg
      721 ccgacgccgt cgcgcgcttc gcccgccgcg gacgcaaggt cctgttcacg ggaacgaaga
      781 agcaggcccg cgacatcgtc aagaagtacg ccgaggagtg cgggtccccg tacgtgaccg
      841 agcgctggct cggcggcacc ctgaccaact tccagaccat ccgcaagagc atccgccgca
      901 tggaggatct catcaagatg gaggaagacg gcacgatgga ccagctcaag aagaaggagc
      961 gtctcatgaa gcggcgcgag cgcgagaagc tggagaaggt gctcagcggc atcaaggacc
     1021 tcgcgcgcct tccgggcgcc ctgttcgtcg tcgacatcaa ccgcgagcac atcgcggtca
     1081 aggaagcgcg ccggctgaac atcccgatca tcgcgctcgt cgacacgaac tgcgatccgg
     1141 acctggtgga cttcccgatc cctgccaacg acgacgccca gcgctcgatc gagctcatca
     1201 cggccgccat ctcgaacgcg gtgtccgaag gcgacaagga gaaggaggtc caggacgcgt
     1261 ccggcaaggc cgaggcgcag aagcgcgccc aggacgtcgc cgagctttcc ggcaaggagt
     1321 cggacgggga ggaagtcgag gtcgcggccg acagcgagtc ggacgactga ttccgcccgc
     1381 cgtgtccgtt ccgggccggc ccgggcggcc cattcataca gatcgatcca ggcgacagcc
     1441 atggccatta ctgccagtga cgtaaagaag ctgcgcgaca tcaccggtgt cggcatgatg
     1501 gactgcaagc gcgcgctcga ggaggccggc ggcgatttcg aggccgccat cgacctcctg
     1561 cgcaagaagg gccagaaggt ggccgccaag cgcgccgacc gcgaggccaa ggagggcgtg
     1621 atcgcgaccg cgtcgaccgg cgacgggcgc accgcgatcc tggtcgaggt caactgtgag
     1681 acggacttcg tcgcgcgcaa cgaggagttc caggcgttcg cctccgcgct cgcgtcgctg
     1741 gttctcgcgg agcgtccggc cgaccgcgag gcgctcctcg cgctgccctt ccagggaggg
     1801 cggaccgtgg agcaggcact gaccgacatg acgggcaaga tcggcgagaa gatcgacgtc
     1861 cgccgcatcg ccgtcgagac gagcacggcc ggccggatcg tctcgtacat ccatcccggc
     1921 gcccgcctgg gcgtgctcgt cgacatcgac gggaacggca acgtcgaggc ggccggccgc
     1981 gacgtggcca tgcaggtcgc cgccctgaac cccgtcgcca ccacgcgcga cgaggtgccg
     2041 caggacgtcc aggagaagga gctcgagatc ggccgcgaga cggccctcaa cgaaggcaag
     2101 cccgagcaga tcgtggaccg catcgcccag ggcaagctcg agcggtactt caaggactcc
     2161 gtgctcgtcg agcagccgtt cgtcaaggac gcctcgatca gcgtcggcgc catgctcaag
     2221 ggagcgggcg cgacggtgaa ggggttcgtg cgcttcgccc tcggcgactg accgtgcgct
     2281 gcggcgcgat tccagggcgt gccggccggt ccggcgcgcc ctttttcttt gtccctcgac
     2341 ccggaccgtg cgcgggggct tcgttgaacc cgagggccgg ttccagtaga ttgggccggc
     2401 ttcgcaaccc gtgctcccga tgtccgacga gtcacccgcc gaccgcccgc tccgttaccg
     2461 gcggatcctg ctcaagctca gcggcgagtc gctcctcggc tccggcgagt acggcatcga
     2521 ccaggacgtg ctgcggctgt acgccgacga gatccgggac ggaatccagg cgggtgccga
     2581 gatcgcgatc gtgatcggcg gcggcaacat cttccgcggc ctgcgcaacg cccacgacgg
     2641 catgacgcgc gcccacgccg actacatggg catgctcgcg accatgatca acgcgatggc
     2701 gctccaggac gcgctcgagc aggtcggcgt gaccaccagg ctgcagtcca gcatcaagat
     2761 ggagcagatc gcggagccgt tcatccggcg gcgcgcgatg cgccacctgg agaaggggcg
     2821 ggtcgtgatc ttcggttcgg gaaccggcaa cccctatttc accacggaca ccgccgcggc
     2881 gctccgcgcc ctggagatcg acgccgacgt gatcctgaag ggcacccgcg tcgacggcgt
     2941 cttcacggcc gacccggagc acgatccagc ggccacgcga ttcaagtcca tccacggcaa
     3001 cgaggtcatc gcgcgcaacc tgcgcgtgat ggacatgacg gccttcaccc tgtgccgcga
     3061 gtcctcgctg ccggtcgtcg tcttcaattt caacgagagg ggcaacttcc tccggctcct
     3121 gcgcggcgag gcggtcggga cgctcgtcca ctgggacgac caccgcaccg ccgagctggc
     3181 cgagcccgcc gccccggtca accggtcgta accatgcttc acgagagcct gcaactcgtc
     3241 atcgacgact cgcgcgagca catggagaaa tccatcgagc acctgcgcgc cgaactgaag
     3301 acgatccgag ccggccgggc cacgcccgcc atgctcgacg gcgtgcgcgt ggactactac
     3361 ggaacgagca ctcccctcgc ccagatggcg agcatctcgg ccccccagcc cgacctgatc
     3421 gtggtccagc cgtgggaccg ctccgtgctg aacgacgtgg agaaggccat ccaggcggcg
     3481 aatctcggca tcaacccgtc gaacgacggc tcgttcatcc gcctgcccgt gccgcccctc
     3541 tccgaggaac gccgccacga cctcgtgaag acggcccgca cgcgcggtga ggacgcgaag
     3601 gtggccatcc gcaacgtccg gagacactcg aaggaccaca tcaagacgat gcagaaggac
     3661 gaaaacctcc ccgaggacat gcgcttcgag gccgaggagg agctgcagcg cctgaccgac
     3721 gagtactcgg agaaggtcga ccacctgctc gggcacaagg aagccgagat catggaggtg
     3781 tgacgtcccc gtccggacgc catacccgtg acccccgcgt gcccgcgccc ggaccgtgag
     3841 gcccaccggg cccgccgtga cgcccgccgc gcgaaaccag cccccgcgtg gggcattcca
     3901 tcccgcccga accccgcgtt cggacccgcc cacggagagg tgcccgagtg gctgaagggg
     3961 cacgcctgga aagcgtgtgt accgtaaccc ggtaccgtgg gttcgaatcc caccc
//
LOCUS       DBNHQZ010000041         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2042682, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000041 DBNHQZ010000000
VERSION     DBNHQZ010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2042682"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="AC16YU_01045"
     CDS             <1..802
                     /locus_tag="AC16YU_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR6063836.1"
                     /translation="YEVSTRPTEGFGDELVRLAREHEYLRFVPHPFDARHVLCVTIDR
                     VAEDTPEDPAHYIGVGRARIAALVAPILRTPWIRTVLGRLLGLARRGYALRIPMSSLL
                     FIRSGVVNSLPGVARVGQLALDRNDWVNMELAVPTERYEAFRRLFDTLKPPTSPFDRH
                     RPYFTCRVVGGATNVLLAPNHGRDVVFTDIHAPPTPESYAFLRRLEEAAAAELGARPH
                     WGKTFFSEQDALRALYPDGNFDAFQDARRRFDPDGVFSNAFTRRVLGD"
     gene            849..1601
                     /locus_tag="AC16YU_01050"
     CDS             849..1601
                     /locus_tag="AC16YU_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063837.1"
                     /translation="MSTPAIVTLFVATYLLSPAPLVAQASHPEPNASVQIATTLPAQI
                     AVPTALTTATGAWALATARAAVEAAATLPIGPQRMELLVQALVAARRAVATDPTDAEA
                     HYWVAAAVGLQADMEGGRTKIRLAEEAWAAATRALEFEPDHAGAHHIQGRLHASVMRL
                     PWVTRTLARHLLGADVLGDASWERAEYHLARAAALAPDDAVNHLEFGLTLRDRGRVEE
                     ARAAFQRALTVPGDRPIDRAPKERARALLDGG"
     gene            1928..>2265
                     /locus_tag="AC16YU_01055"
     CDS             1928..>2265
                     /locus_tag="AC16YU_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HIR6063838.1"
                     /translation="MNATAASAPANVERLREHLASDHPSEHFYVTLLGLSTFASTALH
                     ERVEAGLSYQALERVRQALGVSTADISEFIRVSSRTLARRKESKRLEPDESDRLVRLA
                     RIVGLALRLF"
BASE COUNT          359 a          810 c          731 g          365 t
ORIGIN      
        1 ctacgaggtc tcaacccggc ccaccgaggg attcggcgac gagctggtgc ggctcgctcg
       61 cgagcacgag tacctgcgct tcgtgccgca tcccttcgat gcgcgccatg tcctgtgcgt
      121 gaccatcgac cgcgtggccg aggatacgcc cgaggacccg gcgcactaca tcggcgtggg
      181 acgggcccgc atcgccgcgc tcgtagcgcc catactccgc acgccgtgga ttcggacggt
      241 gctgggccgc ctcctgggac tggcgcggcg gggctacgcg ctccggatcc ccatgtcgag
      301 cctcctcttc atccggtccg gggtggtcaa cagcctcccg ggcgtcgcac gcgtcggcca
      361 gttggccctg gatcggaacg actgggtcaa catggagctc gccgttccca cggagcgcta
      421 cgaggcgttc cgtcggctct tcgataccct gaagccaccc acgtcccctt tcgaccggca
      481 ccgtccgtac ttcacgtgcc gcgtggtcgg cggcgcgacc aacgtcctgc tggcgccgaa
      541 ccacgggcgc gacgtcgtgt tcacggacat ccatgcaccg ccgacgccgg agtcctacgc
      601 cttcctgaga cgtctggagg aggcggctgc ggccgaactg ggggcacggc cccactgggg
      661 aaaaacgttc ttctcggaac aggatgcact ccgggcgctc taccccgacg gcaatttcga
      721 cgccttccag gacgcgcgtc ggcgcttcga cccggacggc gtcttctcca atgcattcac
      781 gcggcgcgtt ctcggtgact gacctgcgcc ccatgtcacg aacgacccat caacccggag
      841 cacacaccat gtcgacccct gcgatcgtta ctctgtttgt cgccacgtac ttgctatcgc
      901 ccgcgccctt ggtggcacag gcgtcgcatc ccgaaccgaa cgcctcggtc cagatcgcca
      961 ccacgctccc ggcccagatc gccgtgccga cggcgctcac caccgctacg ggtgcctggg
     1021 ctctcgccac cgcgcgagcc gcggtggagg cggccgcgac gctaccgatc gggccgcaga
     1081 ggatggagct gctggtccag gcgctcgttg ccgcgcgccg cgcggtggcc accgatccca
     1141 ccgacgccga agcccactac tgggtcgcgg cggcagtcgg cctccaagcg gacatggaag
     1201 gcggacgcac gaagatccgc ctggccgaag aggcttgggc ggcggccacg cgcgctctgg
     1261 agttcgagcc cgaccacgcg ggtgctcatc atattcaggg gcgcttgcac gcgagcgtca
     1321 tgcgcctccc atgggtgacg cgcacgctgg cgcgtcatct gctgggcgcg gacgtactgg
     1381 gcgacgcgag ttgggagcgc gccgagtacc acctggcccg ggcggccgcc ctcgcgcccg
     1441 acgatgcggt gaaccacctc gagttcggcc tgaccctgcg cgaccgaggt cgtgttgagg
     1501 aggcgcgagc agcgttccaa cgcgccctca cggtgcccgg agatcgcccc atcgaccgcg
     1561 cccccaaaga gagagcgcga gcgctcctcg acgggggctg atcgcttaca agcaggaccg
     1621 ttacccctca cgtgcggccg tcgactgtgc cagacaccgt gcaacacgtt ggcggccgtg
     1681 cgaggtcaga gcgtcgaggg gacaacaccc gcatgggagt ggtcgcgagc agtggctgtc
     1741 ctggacgtgc tgaccggaga gacggtcacc ctctggcacc tcgggggcgc ctaccagaac
     1801 gtcgactggc gaccagcctt ggatccctga cgctcttcgt cacgcacggt gggacctggg
     1861 ctgactttga cccacgccac atggccacca tattacagtc acatggcgcg cacgtcagga
     1921 ggaaaccatg aacgccacag ctgcttccgc accagccaat gtcgagaggc tccgcgagca
     1981 cctcgcttcg gaccacccga gtgagcactt ctacgtaacc cttctggggc tttcgacctt
     2041 tgcatccacc gccctccacg agcgggtcga ggccggactg tcctatcagg ccctggagag
     2101 ggtccggcag gccttgggtg tttccacggc cgatatctcg gaattcatcc gggtctcgag
     2161 caggacgctc gcgcgccgga aagagtccaa gcgtttggag cctgacgagt ccgatcgcct
     2221 ggtccggctg gctcgaatcg tcggactcgc gttgaggctc ttcga
//
LOCUS       DBNHQZ010000042         3755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1094265, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000042 DBNHQZ010000000
VERSION     DBNHQZ010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1094265"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..961
                     /locus_tag="AC16YU_01060"
     CDS             179..961
                     /locus_tag="AC16YU_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6063839.1"
                     /translation="MSTDISVDTQDLETRVKDMYREVAENPHGEFHFELGRALAERLG
                     YPPADLDRIPSEAVDSFAGVGHYFGLRGASPGERVLDLGSGSGMDVFVAALHVGADGR
                     VTGLDMTREQLRKAERLRRQAGFDHVDFVEGHLEDLPFGAATFDLVISNGVINLCADK
                     ARVFREAARVLKPGGSLLLSDIVSEVPLPESIVCNVTLWAACIGGALEQGLYARLAEE
                     AGLVVERMVDNAEYGFLSKGARGAVEKYGVKSISLHARKPVG"
     gene            complement(1006..1620)
                     /locus_tag="AC16YU_01065"
     CDS             complement(1006..1620)
                     /locus_tag="AC16YU_01065"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR6063840.1"
                     /translation="MARSPAVQDMSLPPHTVPDRVEIRLPDWVSGTLAEAPACRTDED
                     RMRLAITLSRQNVLRGSGGPFGAAVFEAGGDRPVAVGVNAVERLGNAMLHAEVVALML
                     AQARLGSYTLRMGDGPGFELFTSCVPCAMCLGAALWSGVTRIVCGAAREDAMELGFDE
                     GPVFPSSYLYLEERGIRIDPDVLRGEARAVFAVYRDRGGLVYNG"
     gene            1729..>3755
                     /locus_tag="AC16YU_01070"
     CDS             1729..>3755
                     /locus_tag="AC16YU_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR6063841.1"
                     /translation="MDAFRTAAPFAAILFLSPLALSAQVPQPAYSGWERQLQVEIPRL
                     DARATIDGVLDDEVWSRAARLTGFSQYQPVDGRPAEEPTDVFVWYAPDAIHFGIRATE
                     VHGDAVRATRANRDNISSEDQVQILLDTYDDRRTAFLFAVNPLGVQADGTRSDSFGGG
                     AGGRSATGGGSRMINFLDGNIDLNPDYVFESKGRLTETGYEVEIRIPFKSLRYQESDV
                     QNWGLHILRRSMHSGFQDSWAPAVRANASFLVQSGELVGLHDLRRGLVLEVTPTATGR
                     MDGYQNAAGDWAYDRGGELSADVRWGIRQNLTLNGTVNPDFSQVEADVGQVTLNERFA
                     LFYPEKRPFFLDGLELFDTPGQLIYTRRIVAPVAGAKLAGKVGAWNLATILAADDTTY
                     SATGSTPLFGVARIRRDFGRKAILGGVVTAREDGDDHSRLAGLDVRLYHSRLYYIELQ
                     GVGSWASHGGSSYRGQMFQAVWDRTGRGWGFRYDLSGTSPDFEAAAGFVNRTNIVSAG
                     ASNRLTTYGEPGALIETASSFFHLGRTWAWDGWGEAWDGGAIEGSEAIWPSATLRGGW
                     NVSGAFSRNFYAFDPSFYAGYTVGGEPFVVPGVEDNQWQGSLGVTTPTWQVVSANASY
                     SMGRTPIFREAAPGRSRQFSATVDLRPTQALRASFQVTRLALERQRDDSRFS"
BASE COUNT          591 a         1300 c         1326 g          538 t
ORIGIN      
        1 ccacacgttc cccgaccctg atgccacgcc ctgtcgccca gcggcggcag gacgcgcttc
       61 aaagacctcc agcagggctt tcactttaca ttcatatttg gtatcaaaag gacgttaccc
      121 cgccccccgg gcgattcccg ggcgggtcgt cacccggaat caagacgtgg tcatcagcat
      181 gagtaccgac atcagcgtgg atacccagga cctggagacc cgggtcaagg acatgtatcg
      241 cgaagtggca gagaacccgc acggggaatt tcacttcgag ctcgggcgcg ccctcgccga
      301 gcggctgggc tatccccccg ccgacctgga ccggattccg tccgaggccg tcgactcgtt
      361 cgcgggcgtg ggtcattact tcggcctacg gggcgccagc ccgggcgagc gggtgctcga
      421 cctgggcagc ggctcgggga tggacgtgtt cgtcgccgcg ctgcacgttg gagcggacgg
      481 acgggtcacc gggctcgaca tgacccggga gcagctcagg aaggccgagc gactccgccg
      541 gcaggccggc ttcgaccacg tcgactttgt ggagggccac ctggaggacc tgccgttcgg
      601 ggccgcgacc ttcgacctgg tgatcagcaa cggcgtgatc aacctttgcg cggacaaggc
      661 gcgcgtgttc cgggaggcgg cccgtgtgct gaagccgggc ggctcgctcc tgctctccga
      721 catcgtcagt gaggtgccgc ttccggagtc catcgtctgc aacgtcacgc tctgggctgc
      781 atgcatcggg ggcgccctcg agcaggggct ctacgcacgg ctcgccgagg aagcgggcct
      841 cgtggtggag cgcatggtgg acaacgcgga gtacggattc ctgtcgaagg gggcccgggg
      901 cgccgtggag aagtacggcg tgaagagcat ctcgctgcac gcccgcaagc cggtcggctg
      961 aagccgacgc ctgccgagcc ggcccgcccg gagtcctcct cgccgctacc cgttgtacac
     1021 gagcccgcca cggtcccggt acacggcgaa gaccgcgcgc gcctcgccgc ggagcacgtc
     1081 cggatcgatc cggatgccgc gctcctcgag gtacaggtac gacgagggga acaccggccc
     1141 ctcgtcgaat ccgagctcca tggcgtcctc gcgggccgcc ccgcagacga tgcgcgtcac
     1201 gccgctccac agggcggcgc ccaggcacat ggcgcacggc acgcaggagg tgaagagctc
     1261 gaagccgggc ccgtcgccca tcctcagcgt gtaggagccg agccgagcct gggcgagcat
     1321 gagggccacc acctcggcgt ggagcatggc gttgcccagg cgctcgaccg cgttgacccc
     1381 gacggcgacc gggcggtcgc ccccagcctc gaacacggcc gccccgaagg gacccccgga
     1441 gcctcggagc acgttctggc gcgagagcgt gatggcgagc cgcatgcggt cctcgtccgt
     1501 ccggcaggcc ggcgcctcgg cgagcgtgcc cgacacccag tcgggaagcc ggatctcgac
     1561 gcggtcgggg acggtatggg gcggaaggct catgtcctgg acggctgggg agcgggccat
     1621 gcgggcgccg atcgtgacag gatacggaca aatggaatca cgccggaccg taccttcacg
     1681 cgcccggaac aacaccgcaa gaccttgcca tccacccgcc gacgcacgat ggatgcgttt
     1741 cgtacggccg cgcccttcgc ggcgatcctc ttcctctcgc ccctcgcgct ctcggcgcag
     1801 gtcccccagc ccgcgtattc ggggtgggag cggcagctgc aggtcgagat cccgcgcctc
     1861 gacgcgcgcg cgactatcga cggcgtgctc gacgacgaag tgtggagccg ggcggccagg
     1921 ctcaccgggt tctcgcagta ccagcccgtg gacgggcggc ccgccgagga gccgacggac
     1981 gtcttcgtct ggtatgcccc cgacgccatc cacttcggca tccgggcgac cgaggtccac
     2041 ggggacgccg tgcgggccac ccgggccaac cgcgacaaca tctcgagcga ggaccaggtc
     2101 cagatcctcc tggacacgta tgacgaccgg cggaccgcct tcctcttcgc cgtgaatccg
     2161 ctcggggtgc aggccgacgg gacgcggtcc gactcgttcg gaggcggcgc gggcggccgc
     2221 tcggcgacgg gcggcggaag ccggatgatc aacttcctcg acggcaacat cgacctgaac
     2281 ccggactacg tgttcgagtc gaagggccgg ctcacggaga ccggctacga ggtggagatc
     2341 cggattccgt tcaagagcct gcgctaccag gagtccgacg tccagaactg ggggctccac
     2401 atcctccggc gctccatgca ctcgggcttc caggactcgt gggccccggc cgtccgggcg
     2461 aacgcgagct tcctggtgca gtccggggaa ctcgtgggcc tgcacgacct gcggcgcggg
     2521 ctcgtgctcg aagtgacgcc gaccgcgacc ggacgcatgg acggctacca gaacgcggcc
     2581 ggcgactggg cctacgaccg gggaggcgag ctgagcgccg acgtgcggtg gggcatccgc
     2641 cagaacctga cgctcaacgg cacggtgaat ccggacttct cgcaggtgga ggcagacgtc
     2701 ggccaggtca cgctgaacga gcggttcgcg ctgttctacc cggagaagcg gcccttcttc
     2761 ctcgacggcc tcgagctgtt cgacacgccg gggcagctga tctacacccg gaggatcgtc
     2821 gcgccggtgg cgggagcgaa actggcgggc aaggtcggcg catggaacct ggccacgatc
     2881 ctcgcggccg acgacacgac ctacagcgcg accggcagca cgccgctctt cggcgtcgcg
     2941 cggatccgcc gcgatttcgg caggaaagcc atcctgggcg gcgtcgtcac ggcgcgcgag
     3001 gacggcgacg accattcgag gctcgcgggc ctcgacgtcc gcctgtacca cagccggctc
     3061 tactacatcg agctgcaggg cgtcggaagc tgggcttcgc acggcggctc gtcgtaccgg
     3121 gggcagatgt tccaggccgt ctgggaccgc acggggaggg ggtggggctt ccgatacgac
     3181 ctgagcggca cctctcccga cttcgaggcc gcagcgggct tcgtgaaccg caccaacatc
     3241 gtctcggcgg gcgccagcaa ccgtctgacg acgtacggag agccgggggc cctcatcgag
     3301 accgcgtcct ccttcttcca tctcgggcgg acgtgggcgt gggacggatg gggagaggcc
     3361 tgggacggcg gggcgatcga gggcagcgag gcgatctggc cgtcggccac cctgcgcggc
     3421 ggatggaacg tcagcggcgc gttctcgagg aatttctatg ccttcgaccc gtccttctac
     3481 gccggataca cggtgggcgg cgagccgttc gtggtgccgg gcgtcgagga caaccagtgg
     3541 cagggatcgc tcggggtgac gaccccgacg tggcaggtcg tctccgccaa cgcgtcgtac
     3601 tccatggggc gaacgcccat cttccgggag gccgcgcccg ggcgcagccg ccagttcagc
     3661 gcgaccgtgg accttcgccc cacccaggcg ctgcgcgcgt cgttccaggt gacccgtctc
     3721 gcgctcgagc ggcagcggga cgactcgcgc ttctc
//
LOCUS       DBNHQZ010000043         5774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2855589, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000043 DBNHQZ010000000
VERSION     DBNHQZ010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2855589"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16YU_01075"
     CDS             <1..266
                     /locus_tag="AC16YU_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR6063842.1"
                     /translation="LVRRMDLRVYVDADDDIRLARRIRRDVAERGRSVESVLDQYEAT
                     VRPMHLEFVAPTRGKADIIIPRGGHNEVAVAVLTTYLEGVLPA"
     gene            378..848
                     /locus_tag="AC16YU_01080"
     CDS             378..848
                     /locus_tag="AC16YU_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6063843.1"
                     /translation="MTVFEGSGWMRGYLVGAALLVLVIAGGCGAPREDEQAADPAVTM
                     ADDPVARGRQTFSTYCQGCHGPEGRADGPVAPILSVELSDLTLLNAKYGQFPEELIRA
                     SIDGRAEVQGHGTREMPVWGNIWNDPADPVAEERIRQQIDELVAFVASIQQPVE"
     gene            complement(857..1639)
                     /locus_tag="AC16YU_01085"
     CDS             complement(857..1639)
                     /locus_tag="AC16YU_01085"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6063844.1"
                     /translation="MSDWFVVDIGNSALKAARFGGEGLVASRRVEMLDGRCDLDGAFA
                     EFLASGPVSAAGLASVVPAVTPLVEDRLRSATGRLPLRIDANVRLPFVVAYETPSTLG
                     ADRIAVAAAAWAWRSRNRPAAQVAIAVDAGTAMNVEVVVSTAGGPTYLGGVIAPGPEL
                     MRLALHSGTAQLPTVPLRWPDRIVGSSTERALQAGILPGFVHAVDGIVQQIEQELGHR
                     AAVIATGGWCRLLAEQLPRVNHADPDLVLRGIRDILALNLAG"
     gene            complement(1636..2469)
                     /locus_tag="AC16YU_01090"
     CDS             complement(1636..2469)
                     /locus_tag="AC16YU_01090"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR6063845.1"
                     /translation="MPVTHPPAAPGDLHDLARLLSARAGTGRVGDRIEVHAEVDSTNR
                     LALDRAEAGAGHGLLVVADRQTAGRGRRGRSWSSEAGESLLLSVVVRPPTSTVRWEWL
                     PLATAVAVARAVRETVRAVPAPRVRVKWPNDVLAEGRKVSGILVERRSADGGPGTAVV
                     GIGLNVNQASFPPELAGSATSLRMMSRRRLDRSRVAARLVDELDRVFDAWARGDRSIR
                     ASYRELLEGVGRPVEVRRFDGDDVVPGILDGIDESGCLRLRTPAGDRIFAAGDITFRV
                     R"
     gene            2574..3236
                     /locus_tag="AC16YU_01095"
     CDS             2574..3236
                     /locus_tag="AC16YU_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063846.1"
                     /translation="MLLLALAAGLAAPAAAQTVRPPEIRQEYHRAERAWRTGTNLYEA
                     KVRLDRVLGALPDDAQALLLRARVLLGLDRPREALADARAATALVPDDGHAWLVRAEA
                     AQRTDGARDEALDALGRAAERITDGIEEHVRLSWIAQALSDVDRAESFARIALALGPE
                     EPAADWRLAEVFAEQGRDDDATTLIVRGLTDGVLSGVALRAHPVLAPLAESPAIRPLL
                     GG"
     gene            complement(3169..4014)
                     /locus_tag="AC16YU_01100"
     CDS             complement(3169..4014)
                     /locus_tag="AC16YU_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063847.1"
                     /translation="MVHSGPTRSVLPAALALVLAAAACGTEAGRAPAPGEAGLWAEVE
                     GLDTQAFREAWLRIQARDHGRTTRVEQLDETGRPVAFREWVVRFTAADSQEVVVSADS
                     SGAFRFGMLDAFAPDGAGAPEAVDLAPYVLPDDAPYLSARNREKYAVARLPDSAAFGR
                     PLQRIQVRALPGPGDAEPVRRVLYVLVGEGREVLAVQTEREDAALLFTERSRAYAGMR
                     PDETGGLLPSVSWYETWIRPRFGTDRRFRITTTWTPDDGRDTTRQAGGGSQAIPPAAP
                     AQGAP"
     gene            4088..4912
                     /locus_tag="AC16YU_01105"
     CDS             4088..4912
                     /locus_tag="AC16YU_01105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6063848.1"
                     /translation="MITTDLLDRALDEAVRAALEAGSLIRAEAGRVDARTIADKGIHD
                     LVTEVDAAAERLILTRLSGAFPDFGRLAEEDPASHAAGLPASGFRWIVDPIDGTTNFA
                     HGVPPYAVSIALQHESGILVGVVYDVPHDELFTAVRGGGLRVNGRPARVSRANALGDS
                     LVTTGFPYRSFGHADAYLGVLKAFMERTRGVRRPGSASVDLAYVACGRFDGFFESGLR
                     PWDVAAGYLLVTEAGGRVSDYAGSGDPVFDRQIVASNGHIHPDMLDVLAPMRTARD"
     gene            4981..>5774
                     /locus_tag="AC16YU_01110"
     CDS             4981..>5774
                     /locus_tag="AC16YU_01110"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR6063849.1"
                     /translation="MSTPGNGLLAGKKGVIFGALNESSIAWAIAEAVHREGGRFALSN
                     APIAKRLGTVDALAEKTGSPVLWADATSDVDLAALFESVRQDLGGVDFIVHSIGMGLN
                     VRKNREYDELDYDWYHTTLDISAVSLHRVVHHAEETGALNDGASIVALSYIGAQRTFS
                     KYSEMGDAKALLESIVRSYGYRLGRRGIRINAISQSPTRTTAGSGIAGFDSMYEFAAR
                     IAPLGNADASSCADYTVTLLSDLTRMVTMQTLFHDGGFSTMGISDGL"
BASE COUNT          859 a         2089 c         2031 g          795 t
ORIGIN      
        1 agctggtccg ccgcatggac ctccgcgtgt acgtcgatgc cgacgacgac atccggctcg
       61 cgcgccgcat ccgtagggac gtcgccgagc gcggacggtc cgtggaatcg gtcctggacc
      121 agtacgaggc caccgtccga cccatgcacc tcgagttcgt cgctcccacc aggggcaagg
      181 ccgacatcat catcccccgg ggcggccaca acgaggtggc cgtggccgtg ctcaccacgt
      241 acctcgaagg cgtcctgccg gcctgatttt gggccctgga agcctcttcc ggggcgatcg
      301 cgccttgcgg gacccgctgc tggagcgtaa tttcgccgag cagtgccttg agcaacgagc
      361 aacgtcgggg ggacgccatg accgtcttcg aaggcagcgg gtggatgcgc ggatacctgg
      421 tcggagcggc actcctggtt ctcgtgatcg ccggcggatg cggcgctccc cgggaggatg
      481 aacaggcggc ggatcccgcc gtaacgatgg cggacgatcc cgtcgcgagg ggccgtcaga
      541 ccttctcgac ctactgccag gggtgtcacg ggccggaggg tcgtgcggac ggccccgtcg
      601 cccccatact gagcgtcgaa ctgtcggacc tgacgctcct caacgcgaag tacgggcagt
      661 tccccgagga gctgatccgg gcctccatcg acggccgggc cgaggtgcag ggtcacggga
      721 cccgcgagat gccggtatgg ggaaacatct ggaacgatcc cgccgatccg gtggcggagg
      781 agcgcatccg gcagcagatc gacgagctcg tcgcgttcgt cgcgtccatc cagcagccgg
      841 tcgaataggt actccctcag ccggcgagat tcagggccag gatgtcgcgg atgccgcgaa
      901 ggaccaggtc cgggtccgca tgattcacgc ggggaagctg ctccgccagg aggcggcacc
      961 atccgcccgt tgcgatcacg gccgcccggt gtccgagctc ctgctcgatc tgctgcacga
     1021 ttccgtccac tgcgtgcacg aatcccggca ggatgcctgc ctgaagggcc cgctccgtcg
     1081 atgagcccac gatgcggtcc ggccaccgga gcgggacggt cggaagctgt gcggtccccg
     1141 aatggagtgc gaggcgcatc agctcgggac cgggcgcgat cacgccgccc aggtaggtcg
     1201 gccccccggc cgtggagacc acgacttcca cgttcatcgc ggtgccggcg tccacggcga
     1261 tcgccacctg ggcggccggc cggtttcggc tccgccacgc ccaggccgcg gccgcgacgg
     1321 caatgcgatc cgctccgagg gtggacggcg tctcgtacgc gacgacgaac ggcaggcgca
     1381 cgttcgcgtc gatccgcagg ggaagcctgc ccgtcgcgga gcgcagccgg tcctcgacga
     1441 gcggggtgac cgccggcacg accgacgcca gtccggccgc ggaaaccgga cccgaggcca
     1501 ggaattccgc gaatgccccg tccaggtcgc accgtccgtc gagcatctcg acacggcggc
     1561 tggcgaccag cccctctccg ccgaaccggg cggccttcag ggcgctgttg ccgatatcga
     1621 ctacgaacca gtcgctcatc ggacgcggaa cgttatgtcc cccgcagcga agatacgatc
     1681 gcccgccggg gtgcgcagcc ggagacatcc cgactcgtcg atcccgtcga gaatccccgg
     1741 aaccacatcg tctccatcga accgacggac ctcgaccgga cggccgacgc cctcgagcag
     1801 ttcccggtag gacgcccgga tcgatcggtc tccgcgggcc caggcgtcga acacccggtc
     1861 gagctcgtcg accagtcggg cggcgacccg ggaccggtcc aggcgccggc gggacatcat
     1921 ccggagcgac gtggccgagc cggcgagttc gggcggaaag ctcgcctggt tgacgttcag
     1981 ccctatcccc accaccgccg tgcccggccc tccgtctgcg gaccggcgct cgacgagaat
     2041 gccggacacc ttgcggccct ccgcgagcac gtcgttcggc cacttgaccc ggacccgcgg
     2101 agcgggcacc gcgcggaccg tctcgcggac tgcgcgggcg accgcgaccg ccgtcgccag
     2161 cggcagccac tcccagcgga ccgtgctcgt cggggggcgg acgaccacgg acagcaggag
     2221 gctctcgccc gcctccgatg accaggacct cccccgtcgg ccgcgaccgg cggtctggcg
     2281 atccgccacg accaggagtc cgtggccggc gcccgcctcc gcccggtcca gcgcgagccg
     2341 gttcgtggag tcgacttcgg cgtgcacctc gatgcgatct cccacgcggc ccgtgccggc
     2401 gcgtgcggag agcagccgcg cgagatcgtg caggtcgccg ggagcagccg gggggtgcgt
     2461 gacgggcatg gcgcaggggg atcgtgggca aggtacggcg gcgtgccgaa cgcttccggc
     2521 cgggcggcgt aagcccccgg ttacacgacg ccggaaccgt gacccgccac ccgatgcttc
     2581 tcctggcact cgccgccggt ctcgccgcgc ccgccgccgc gcagacggtg cgcccgccgg
     2641 agatccgaca ggaatatcat cgcgcggagc gggcatggcg gaccggaacg aacctgtacg
     2701 aggccaaggt gcgcctggac cgcgtgctcg gcgcgttgcc cgacgatgcc caggcgctcc
     2761 tgctgcgggc acgcgtcctg ctcggcctgg accggccgcg ggaggcgctg gcggatgccc
     2821 gggctgcgac ggcgctcgtt cccgacgacg ggcacgcgtg gctcgttcgc gcggaggcgg
     2881 cgcagcgcac ggacggggcg cgcgacgagg ccctcgacgc cctggggcga gccgccgagc
     2941 ggatcaccga cggaatcgag gagcacgtga ggctctcgtg gatcgcccag gccctctcgg
     3001 acgtcgaccg cgcggagtcc ttcgcccgca tcgcactcgc gctgggcccc gaggagccgg
     3061 cagcggactg gcgcctggcc gaggtcttcg cggagcaggg ccgcgacgac gacgcgacca
     3121 ccctgatcgt ccgggggctc accgacggcg tgctgtcggg cgtggccctc agggcgcacc
     3181 ctgtgctggc gccgctggcg gaatcgcctg cgatccgccc cctgcttggc gggtagtatc
     3241 gcggccgtcg tccggtgtcc aggttgtcgt gatccggaac cgccggtccg tgccgaatcg
     3301 cgggcggatc caggtctcgt accacgagac cgacggcagc aggccaccgg tctcgtcggg
     3361 tcgcatgccc gcgtaggcgc ggctccgctc ggtgaagagc agtgcggcgt cctcgcgctc
     3421 ggtctgcacg gccaggacct ccctcccctc cccgaccagg acgtacagga cgcgacgcac
     3481 gggctcggcg tcccccggcc cgggaagggc gcgcacctgg atgcgctgca ggggcctccc
     3541 gaacgccgcc gagtcgggca gccgggcgac cgcgtacttc tcccggtttc tcgcactcag
     3601 gtacggggca tcatctggca ggacgtacgg tgcgaggtcc acggcttccg gtgctccggc
     3661 cccgtccggc gcgaaggcgt cgagcatgcc gaaccggaac gctcccgacg agtcggcgct
     3721 cacgacgacc tcctgggagt cggcggccgt gaagcgcacg acccactcgc ggaaggccac
     3781 cgggcgtccg gtctcgtcca gctgctcgac gcgcgtcgtc cgcccgtggt cacgtgcctg
     3841 aatgcgcagc caggcctcgc ggaacgcctg cgtgtcgagc ccctccacct ccgcccagag
     3901 ccccgcttcg cccggcgccg gcgcccgccc ggcctccgtt ccgcaggccg ccgcggccag
     3961 cacgagggcc agcgcagcag gaaggacgga acgcgtcggg ccggagtgaa ccatcgggct
     4021 tcggtcgggt actcggtgcg ccggaggccg tccggcggat ggccaagata gacaatcgga
     4081 ccgcgcgatg atcacgaccg acctgctcga ccgggcgctc gacgaggccg tgcgagccgc
     4141 cctcgaggcc ggaagcctga tccgtgccga ggcgggacgc gtcgatgccc gcacgatcgc
     4201 ggacaagggt atccacgacc tggtcaccga ggtcgatgcc gcggctgaac ggctcatcct
     4261 cacccggctg tccggggcat tcccggattt cggcaggctc gccgaggagg accccgccag
     4321 ccacgcggca gggctgcccg cgtcgggctt ccgctggatc gtggacccga tcgacgggac
     4381 gaccaacttc gcccacggcg tacctcccta cgccgtaagc atcgccctgc agcacgagag
     4441 cggcatcctc gtcggcgtcg tgtacgacgt cccgcacgac gagctcttca cggccgtgcg
     4501 gggcggcggg ctgcgggtga acgggcggcc ggcccgcgta tcgcgcgcga atgcgctcgg
     4561 agacagcctg gtcacgaccg gcttcccgta ccggtcgttc ggtcacgcgg acgcgtacct
     4621 gggcgtgctg aaggcgttca tggagcgcac acggggggtc cggcggccgg gttcggcgag
     4681 cgtggacctc gcatacgtgg cgtgcggccg cttcgacggc ttcttcgaat cgggcctgcg
     4741 gccgtgggac gtcgccgccg ggtacctgct cgtcacggag gcgggcggcc gggtgtcgga
     4801 ctacgccgga agcggcgatc ccgtcttcga tcgccagatc gtcgcctcga acgggcacat
     4861 ccatccggac atgctcgacg tcctcgcgcc gatgcgcacg gccagggact gacggcgcgt
     4921 cggcggcgtt tcgtaccttc ccggccccgc cacggttcag agccaccatc cgagagcatc
     4981 atgtcaaccc cgggcaacgg actgcttgcc ggcaagaagg gcgtcatctt cggcgccctg
     5041 aacgagagca gcatcgcatg ggccatcgcc gaggccgtcc accgggaagg tggacgtttc
     5101 gcgctctcga acgctcccat cgcgaagcgc cttggaaccg tagacgccct cgccgagaag
     5161 acgggcagcc cggtcctgtg ggcggacgcg acgagtgacg tggaccttgc cgccctgttc
     5221 gagagcgtga ggcaggatct cggcggtgtg gatttcatcg tccactcgat cgggatgggc
     5281 ctgaacgtcc gcaagaaccg cgagtacgac gagctcgact acgattggta ccacacgact
     5341 ctggacatct cggcggtcag cctgcaccgc gtcgtacacc acgcggagga gacgggcgcc
     5401 ctgaacgacg gcgcgtccat cgtcgcgctc tcgtacatcg gcgcgcagcg cacgttctcg
     5461 aagtactcgg agatgggcga cgccaaggcc ctgctcgaga gcatcgtgcg gtcgtacgga
     5521 taccgcctgg ggcgtcgcgg catccggatc aacgcgatct cgcagagccc cacgcggacg
     5581 accgcgggct cgggcatcgc cgggttcgac tccatgtacg agttcgccgc gcgcatcgcg
     5641 ccgctcggca acgccgacgc ctccagctgc gccgactaca ccgtcacgct cctgtcggac
     5701 ctcacccgca tggtcacgat gcagacgctg ttccacgacg gcgggttcag cacgatgggg
     5761 atctcggacg gcct
//
LOCUS       DBNHQZ010000044         4380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1231114, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000044 DBNHQZ010000000
VERSION     DBNHQZ010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1231114"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="AC16YU_01115"
     CDS             <1..1719
                     /locus_tag="AC16YU_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6063850.1"
                     /translation="GGNAVDAACAMIAATATMWDVLHWGGETQALVWNPHTRKVTGIN
                     ALGVAPTGATAEFFRSKGMDHPPAYGPLAAVTPGTPGGLMTMLAEYGTLSLAEVLAPA
                     IQMADGYPIEAQTANSIERAKDWLKQWKYTPATMLPHLGEAREAPFPGEVFRQADLAA
                     TLRKLVAAEAEAIAAGRSRKEAIQAAQDRFYRGDIAEELVRGVQEDGGLITLEDLAGW
                     SVKIEEPVSTTYRGIEVFKLTHWTQGPVLLQALNMLRDMDLKAMGYNSARYIHALYQV
                     MNLAYADRDFYYGDPSFPPEEPIRGLLSGEYARERAAAIDWERNDPDARPGDPYPFQG
                     GTNPFAELLERWHTTGVEPAPGAPGGSPAPAHDQRPGGPSDEAFFSGTTSIQAADAEG
                     WVVSVTPSGGWIPAVIAGRTGVGLSQRMQSFVTDPAENPFNVVEPGKQPRVTLTPSLA
                     LKDGAPFLSFAVQGGDGQDQWLLQFFLNVVEFGMTVQEATEAANISSFQMRESFDRHV
                     SRPGRLQLRDDTPPWVRDELRRMGYTLTFDDRTSGPINAIWFDREHGTLWGGSSNHGE
                     DYGIAW"
     gene            complement(1764..2366)
                     /locus_tag="AC16YU_01120"
     CDS             complement(1764..2366)
                     /locus_tag="AC16YU_01120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6063851.1"
                     /translation="MAEFYSPQSRSLQDRFDTRRLADRLREVKVHDRFTADDRAFIER
                     LDMFFLASVDADGRPTCSYKGGDPGFVSVVDEQTLAFPNYDGNGMYLSMGNVAATGAV
                     GLLFIDFERQRRMRVDGTARILMDDPLLERHPEAQFMVRVEAGNIYPNCPRYIHRYEL
                     AERSSFVPRPRTRTPVPAWKRSDWATDVLPAGDPAREPEP"
     gene            complement(2594..3328)
                     /locus_tag="AC16YU_01125"
     CDS             complement(2594..3328)
                     /locus_tag="AC16YU_01125"
                     /inference="COORDINATES: protein motif:HMM:NF039186.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HIR6063852.1"
                     /translation="MSALYLVVNLAVLSVPLAFSWHPRLRFVDRWRDFVPACLAVAAG
                     FIAWDALFTAWGVWGFEDRYLLGARVAGLPVEEWLFFLCVPYACVFTYHAFGVLGAGG
                     PGRRTALALTGAALGLCILLTALFPARLYTAVTAALLAVLLAGLLWTRPSWLGRLWLS
                     LAVLTVPFILSNGVLTGLRFWTYPVLNARPEQVAPQVVWYDNAHNLGVRLFSIPLDDF
                     LYAALLIGLNVAGMEWLAARRRRRGE"
     gene            complement(3336..3839)
                     /locus_tag="AC16YU_01130"
     CDS             complement(3336..3839)
                     /locus_tag="AC16YU_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6063853.1"
                     /translation="MSILVNILIVIGTFAAMEAVAWLAHRFLMHGPMWYFHRDHHNHE
                     PGFFEKNDTFVLIFAIPSAWFFYTGTMNGDFRLWIGLGILLYGICYVFVHDIFIHQRF
                     KWLRGTNSVYLTAIRKAHLMHHKHLGPEDGECFGMLVPPPKYFAEARTAVSARKAARS
                     GRQASNA"
     gene            complement(3836..>4380)
                     /gene="idi"
                     /locus_tag="AC16YU_01135"
     CDS             complement(3836..>4380)
                     /gene="idi"
                     /locus_tag="AC16YU_01135"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005290867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR6063854.1"
                     /translation="ERVVLVDSDDRPLGEMEKLQAHREGALHRALSVFVFNGKGELLL
                     QRRAAAKYHSGGLWTNTCCSHPRPGEPVDRAAARRLREEMGLECELEPRFAFVYRSEL
                     DGGLVEHELDHVFLGRSDAEPDPDPAEVSEWRYASPDDIRHELEQAPDRFTAWFRICF
                     ESAVDAARHPSESTREILPT"
BASE COUNT          713 a         1507 c         1568 g          592 t
ORIGIN      
        1 ggcggcaacg ccgtcgacgc cgcgtgcgcc atgatcgcgg ccaccgccac gatgtgggac
       61 gtgctgcact ggggcggcga gacgcaggcg ctcgtctgga acccgcacac gcgcaaggtg
      121 accggcatca acgcgctggg cgtcgcgccc accggcgcga ccgcggagtt cttccggtcg
      181 aagggcatgg atcacccgcc ggcctacggt ccgctcgcgg ccgtcacgcc gggcacgccg
      241 ggcgggctca tgacgatgct cgccgaatac ggcacgctct cgctcgccga ggtgctcgcc
      301 cccgccatcc agatggccga cggctacccg atcgaggcgc agacggcgaa ctccatcgag
      361 cgggcgaagg attggctcaa gcagtggaag tacactcccg ccaccatgct tccgcacctg
      421 ggcgaggcgc gcgaagcgcc gttcccgggc gaggtcttcc ggcaggcgga tctcgccgcg
      481 accctccgga agctggtcgc ggccgaagcg gaggcgatcg ccgcgggcag gagccggaag
      541 gaagccatcc aggccgcgca ggaccgcttc taccggggcg acatcgccga ggagctcgtg
      601 cgcggcgtgc aggaggacgg cgggctcatc acgctcgagg acctcgccgg ctggagcgtg
      661 aagatcgagg agccggtgag caccacgtac aggggcatcg aggtcttcaa gctcacccac
      721 tggacgcagg ggcccgtgct gctgcaggcg ctgaacatgc tgcgcgacat ggacctgaag
      781 gcgatgggct acaacagcgc ccgctacatc cacgcgctct accaggttat gaacctggcc
      841 tacgcggacc gcgacttcta ctacggcgac ccttcgttcc cgccggagga gcccatccgg
      901 ggcctgctct ccggcgagta cgcccgagag cgcgcagccg cgatcgactg ggagcgcaac
      961 gaccccgacg cgaggccggg cgacccgtat ccgttccagg gcggcacgaa ccctttcgcg
     1021 gagctgctgg agcggtggca cacgacgggg gtcgagcccg cgcccggggc cccgggcggc
     1081 tcccccgccc cggcgcacga ccagcggccg ggcgggccgt cggacgaggc gtttttctcc
     1141 ggaacgacgt cgatccaggc ggccgacgcc gagggctggg tcgtgtccgt gacgccgagc
     1201 ggcggctgga tccccgcggt gatcgcgggg cggacgggcg tcggcctgag ccagcgcatg
     1261 cagagcttcg tcaccgatcc ggccgagaac cccttcaacg tggtcgagcc gggcaagcag
     1321 ccccgcgtca cgctcacgcc gtcactcgcg ctgaaggacg gcgcgccctt cctgtccttc
     1381 gccgtgcagg gcggcgacgg gcaggaccag tggctcctgc agttcttcct gaacgtggtc
     1441 gagttcggga tgaccgtgca ggaggcgacc gaggcggcca acatcagctc gttccagatg
     1501 cgcgagtcgt tcgatcggca tgtctccagg cccggacgtc tgcagctgcg cgacgacacc
     1561 ccgccctggg tccgagacga gctgcgccgc atgggctaca cgctcacgtt cgacgaccgg
     1621 acgtcgggtc ccatcaacgc catctggttc gaccgcgagc acgggaccct gtggggcggc
     1681 tcgagcaacc acggcgagga ttacgggatc gcctggtaag gatcccgcgc gccgccgcac
     1741 gggctgaacg tccggccggg cgttcagggc tccggttcgc gcgcagggtc gccggccggc
     1801 aggacgtcgg tcgcccagtc ggaccgcttc caggccggga cgggcgtccg tgtgcgaggc
     1861 cgcgggacga acgaggagcg ctcggccagc tcatagcggt ggatgtagcg gggacagttc
     1921 gggtagatgt tcccggcctc gacccggacc atgaactgcg cctcgggatg gcgttcgaga
     1981 agcgggtcgt ccatcaggat cctcgccgtc ccgtcgaccc gcatgcggcg ctgccgctcg
     2041 aagtcgatga agagcagccc gaccgcccct gtcgcggcga cgttccccat cgagaggtac
     2101 atcccgtttc cgtcatagtt gggaaaggcg agcgtctgct cgtccacgac ggagacgaag
     2161 cccgggtccc cgcccttgta ggagcaggtg ggccgcccgt ccgcgtcgac gctcgcgagg
     2221 aagaacatgt ccaggcgctc gatgaaggcg cggtcgtccg cggtgaatcg gtcgtggacc
     2281 ttcacttcgc ggagacggtc ggccaggcgg cgcgtgtcga agcggtcctg gagggatcgg
     2341 ctctggggcg agtagaattc ggccatggtg cacctctcgt accgtgccgg ttccggtgtt
     2401 ctgccgcccg cgcggcgccg cgccgtgccc ggttccgggg agggcacgcc gcccgccgtg
     2461 caggcctgga ttgtgccgtg cgccgcctcg ccgcccgccg tgtcccgcga acgggtcgcc
     2521 gccgccccgt aatggtaacg gatccggggc taccggggca acggttcgcg ccgcagccag
     2581 gcgcgcttcg gggtcactcc ccacgccgcc gccgccgcgc cgccagccac tccatcccgg
     2641 ccacgttcag gccgatgagc agggccgcgt acaggaagtc gtcgagcggg atgctgaaca
     2701 gccgcacgcc caggttgtgc gcgttgtcgt accagacgac ctgcggggcg acctgctccg
     2761 gccgggcatt cagcacgggg tacgtccaga aacggagccc cgtgagcacg ccgttcgaga
     2821 ggatgaaggg caccgtgagc acggcgagcg agagccagag ccggccgagc caggagggcc
     2881 gcgtccacag gagtccggcg agcagcacgg cgagcagcgc ggccgtcacc gccgtgtaga
     2941 gccgcgccgg gaagagcgcg gtgagcagga tgcacaggcc gagggcggcg cccgtcaagg
     3001 cgagcgcggt gcggcggccc ggcccgccgg cgccaagcac cccgaacgca tggtacgtga
     3061 agacgcaagc gtacgggacg cacaggaaga agagccactc ctcgaccgga aggccggcca
     3121 cgcgggcccc caggaggtac cggtcctcga agccccagac cccccacgcg gtgaacaggg
     3181 cgtcccaggc gatgaagccc gccgcgaccg cgaggcacgc cgggacgaag tcgcgccagc
     3241 gatccacgaa gcggagcctc gggtgccagc tgaaggccag cggcaccgag agcaccgcca
     3301 ggttcacgac caggtagagc gcggacacgt ccggctcagg cgttcgaagc ctgtcgaccc
     3361 gagcgtgccg ccttccgcgc cgagaccgcg gtgcgcgcct cggcgaagta cttcgggggc
     3421 ggcaccagca tgccgaagca ttccccgtcc tcgggaccga ggtgcttgtg gtgcatgagg
     3481 tgcgccttac ggatggccgt caggtacacg ctgttcgtcc cccgaagcca cttgaaccgc
     3541 tggtggataa agatgtcgtg cacgaacacg tagcagatcc cgtagaggag gatcccgagg
     3601 ccgatccaca gccggaagtc cccgttcatc gtgcccgtat agaagaacca ggcgctcgga
     3661 atcgcgaaga tcagcacgaa agtgtcgttc ttctcgaaga agccgggctc gtggttgtgg
     3721 tggtcccggt ggaaatacca catcggaccg tgcatcagga agcgatgcgc gagccaggcc
     3781 acggcctcca tggccgcgaa cgtgccgatg acgatgagga tgttgacgag gatgctcatg
     3841 tcggaaggat ttcccgggtg gattcggatg gatgccgggc ggcgtcgacc gcgctctcga
     3901 agcagatgcg gaaccacgcg gtgaagcgat cgggggcctg ctcgagctcg tggcggatgt
     3961 cgtcgggcga ggcgtagcgc cactcggaga cctccgcggg atccgggtcc ggctcggcgt
     4021 cggaccgccc caggaacacg tggtcgagct cgtgttcgac gagccccccg tcgagctcgc
     4081 tccggtacac gaacgcgaac cgcggctcga gctcgcactc gaggcccatc tcctcgcgca
     4141 gacggcgcgc ggcggcccgg tcgaccggct cgccgggcct cgggtggctg cagcacgtgt
     4201 tcgtccacag gcccccgctg tggtacttgg cggcggcgcg gcgctggagc agaagctctc
     4261 ccttcccgtt gaacacgaaa accgacagcg cgcggtgcag cgcgccttcc ctgtgggcct
     4321 gaagcttctc catctcgccc aggggacggt cgtcggaatc gacgagcacg acgcgttctt
//
LOCUS       DBNHQZ010000045         5622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2856379, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000045 DBNHQZ010000000
VERSION     DBNHQZ010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2856379"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(669..752)
                     /locus_tag="AC16YU_01140"
     tRNA            complement(669..752)
                     /locus_tag="AC16YU_01140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Leu,seq:gag)
     gene            902..2641
                     /locus_tag="AC16YU_01145"
     CDS             902..2641
                     /locus_tag="AC16YU_01145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6063855.1"
                     /translation="MRVPRLLPLLALPLAIVACRPAGRAEPCGDDLASFEGAFGAEPA
                     DAVVEFRARDGELLMQPALWSGPMVLGRDRADSFVVASHPRFGATFVRDGRGCVSTAR
                     IRGLGPEGSWRRLDPEHPRPVQLLTSGRPSEAASAYLASGTADPADLEQVASMFLRSM
                     PSHAGDAAVFLETLTDVLPEAAGLHAAHGDALLALGRRAEARAAWERALALDAGTERA
                     RKALERLGVLAPAETGGWTLPFDLEEALAPPEGAEIEAALAAADARNLDPDEPRIVLE
                     RGITLNGSPHELRVIEHGVHGSRHLGVVLVPEGVPPGGLPVLVEAKGVSPSFFPLEVP
                     GGLTSPAFMEGVPVIYAAPGYRGERIAVGPDTLLSEGDRSDAWDGAADDLVAFLRVAL
                     AATPEADTSRVCVFGRSRGGTVALLAGMREPRIDCVVSWAAPTDWFRLMDLEGWTQQE
                     LVADGLRHRAGPGATGGQFINYFLRRAIDGGAGLGETRRRMILSSPLYFAGRLPLTQA
                     HWGLEDSIVPPVNGREFFERWNAASRPAACLDARFHPDAGHDQDRQLAPRQSRAFILA
                     AFAGPDAATACRP"
     gene            complement(2651..4300)
                     /locus_tag="AC16YU_01150"
     CDS             complement(2651..4300)
                     /locus_tag="AC16YU_01150"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6063856.1"
                     /translation="MRRFLPVLLALAALSCREAAPPPNIVFIFSDDHGRQAVGAYGSV
                     INETPNIDRIAREGALFTNALVTNSICAPSRATVLTGRYNHVNGQLTNSETFDGSQET
                     FPKLLQQAGYETALVGKWHLKSAPTGFDFWRVLIGPGPYYNPPIGSQGDTVRVPGYTT
                     DLVTDIALEWLRDGRDPSRPFLLMFQHKAPHRNWQPGPDHLADYEDVDVPMPATFFDD
                     YSGRTRAAAEATMSVARNLSAHDLKIAEQAELDAEQRRAWDAVYGPRNETVRAAALSD
                     SALTRWKYQRYAKDYLRSVQSLDDNIGRMLDYLDEAGLSDNTIVVYMSDQGWYLGEHG
                     WYDKRWMYEPSLRTPFLVRWPGVAAASAVSDALVSNVDVAPTLLDAAGVAVPDGMQGA
                     SILPLLRASRSPETPAPRPSLELVDAGPPGAEVPSYGMPRDPAPTFGTPAGWRRSFYY
                     HYYEYPGWHCVRRHYGVRTDRYKLIHFYTLGEWELFDLAEDPDELRSVYDDPDYANVR
                     ADLHAELDRLRSELAVPEDDRPDGEGECTLGGEGWMGYGAE"
     gene            4343..5107
                     /locus_tag="AC16YU_01155"
     CDS             4343..5107
                     /locus_tag="AC16YU_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIR6063857.1"
                     /translation="MSSDQRFDDLAVLYVGLAHGIDGRLHFQELTQIESHLERWRHPG
                     ERRNVSSVVDEVVRAYDPIRVIDAVQRLGRQLSEREQRAALDDLSAIALADRRFLKEE
                     AEYIGRVAFDWGLQLDETEEGALWTVLRSRGDADPRALAMDLALLYLAVAHRPDDDIS
                     DRELAAIRGAMAGWLPGLGDEQVERLVRSALAAFARGMAEGGAEMIVRRVRATLPIHQ
                     RQAVVRDIRSIAGADGDPTVEAGLLVHWLEDELEAR"
BASE COUNT          825 a         1968 c         1972 g          857 t
ORIGIN      
        1 cgatccggtc ctcgttgccc gactgcgacc acgtccccgc gctgtgggtc cacgtgctct
       61 tggctatgga ttcggtcgca tcgggccact gcgccgccac gtacaggtag cccccgtcgg
      121 ccatcgccct gagcgtgaca ccggatgcgg tcaccagctc ggtggcaggc cactccccgc
      181 tatcgagcac gccatcaaga acggccgagc cggtgccgac agccagtccc tggcccgcca
      241 cacctgaagg caggaatcca ccgaccaaga tcagaagaga tagtctcgtt acccacatga
      301 cagcctccaa aggattgatt tggacgtaag gggtcacacc acgacaatga ggtccattga
      361 atcgaattgg atgacagtca ttgtcgcatc agcctgcagg ggccaggctc caataatcgt
      421 aggtagatcc cctgcattct ttcggctgca ccacggtgaa ccctggatgc gaccgccatg
      481 aaccggtggc gcccgccgca tcgggtccgg cgaggagtta cggcacggtc ggtcctgacg
      541 tcaccgtcga ttctcgggac attcgggcgc tgccggaggc tccggatctc aagaaaaaca
      601 ggaccgcccg aaacggacga tcctgctatg aaaggggcat caccgggatg ccctcgatct
      661 cgaaggtgtg cccaggactg gactcgaacc agcacgccct tgcgggcacc gccccctcaa
      721 gacggcgtgt ctacctattc caccacctgg gcaggggcgc ggaagatacc accggccggg
      781 ccggtgctgt caacctgcat ctgaatttaa cggcggccat cgaaccgcgg atcccggccg
      841 gacgcgcccg taaccccggg cgcggggtgt cgtaccgttc gcaacgcctg agatcagtcc
      901 catgcgcgtt ccgcgccttc ttccgctcct cgccctgccg ctcgcgatcg tggcttgccg
      961 gccggcgggg cgggcggagc cgtgcggcga cgacctcgcc tcgttcgagg gcgccttcgg
     1021 tgccgagccg gccgacgccg tggtcgaatt ccgcgcccgg gacggcgagc tgctgatgca
     1081 gccggcgctg tggtccggtc ccatggtcct cgggcgcgac cgggcggatt ccttcgtggt
     1141 cgcctcgcat ccgcggttcg gggccacgtt cgtgcgcgac ggccgcggat gtgtttcgac
     1201 ggcgcgcatc cgcgggctcg ggccggaggg ctcgtggcgc cgcctggatc ccgagcaccc
     1261 ccgccccgtg cagctgctca cctccggacg gccgtccgag gcggcctccg cgtacctggc
     1321 gtccggaacg gccgatcccg ccgatctgga gcaggtcgca tccatgttcc tgcggagcat
     1381 gccatcgcac gcgggtgatg ccgcggtctt cctcgagacc ctcaccgatg tgcttcctga
     1441 agcggcggga ctgcatgcgg cgcacggcga tgcgctcctc gcgctgggcc ggcgtgcgga
     1501 ggcccgcgcc gcctgggagc gcgcgctcgc gctcgacgcc ggcaccgaac gcgcacggaa
     1561 ggccctcgag cgcctgggcg tgctcgcgcc cgccgaaacc ggcggctgga cgctgccctt
     1621 cgaccttgaa gaagcgctcg ccccgccgga aggggccgag atcgaggcag cgctcgcggc
     1681 ggcggacgcc cggaacctcg atcccgatga gccgcggatc gtcctcgagc gcgggatcac
     1741 gctgaacggc tcgccgcacg agctccgcgt catcgagcac ggggtgcacg gctcgcggca
     1801 tctcggcgtg gtgctggtcc ccgaaggcgt tccgcccggc ggcctgcccg tgctggtcga
     1861 ggcgaagggc gtctcgccga gcttcttccc cctcgaagtg ccgggcggcc tgacgagccc
     1921 cgccttcatg gagggcgtcc ccgtcatcta cgcggcgccc gggtaccgcg gcgagcggat
     1981 cgcggttggt ccggacacgc tcctctccga aggggaccgc tccgacgcct gggacggcgc
     2041 ggcggacgac ctcgtcgcct tcctgcgcgt cgcgctcgcc gcgacgcccg aggcagacac
     2101 ctcgcgggtc tgcgtgttcg gccgcagccg cggcggaacc gtggcactcc tggccggcat
     2161 gcgcgagccg aggatcgact gcgtggtctc gtgggccgcc ccgacggact ggtttcgcct
     2221 catggacttg gaagggtgga cgcagcagga gctcgtggcc gacgggctcc gacaccgcgc
     2281 cgggcccggc gcgaccgggg ggcagttcat caactacttc ctgcgccggg cgatcgacgg
     2341 cggggcgggc ctcggggaga cgcgccgccg catgatcctc tcgtcgccgc tgtacttcgc
     2401 cggtcgcctc ccgctcacgc aggcgcactg gggcctggag gactcgatcg tgcccccggt
     2461 gaacggccgg gaattcttcg aacggtggaa cgcggcgtcg aggcccgctg cgtgcctgga
     2521 cgcgcgcttc catccggacg ccggccacga ccaggaccgc cagcttgcgc cccgccagtc
     2581 gcgcgcgttc atcctggccg cgttcgctgg tccggacgcc gcgaccgcat gccggccgtg
     2641 acggcggcgg tcactccgcc ccgtacccca tccacccctc gccgccgagg gtgcactcgc
     2701 cctcgccgtc cggccggtcg tcctcgggga cggccagttc cgatcgcagc cggtcgagct
     2761 ccgcgtgcag gtcggcccgg acgtttgcgt agtccgggtc gtcgtagacg ctccgaagct
     2821 cgtccgggtc ctcggccagg tcgaagagct cccactcgcc gagcgtgtag aagtggatca
     2881 gcttgtaccg gtcggtgcgc acgccgtagt gccggcggac gcagtgccaa cccggatact
     2941 cgtaatagtg gtagtagaag ctccggcgcc acccggcggg tgtcccgaac gtgggcgcgg
     3001 gatccctggg catcccgtac gacggaacct cggcgccggg cgggccggcg tccacgagct
     3061 ccagggaggg ccgaggggcc ggagtctccg gcgagcgcga ggcgcgcaga agcggcagga
     3121 tcgacgcccc ctgcatgccg tccggcacgg ccacccccgc ggcgtcgagc agggtggggg
     3181 cgacgtccac gttcgacacc agcgcgtcgc tcacggccga ggccgccgcg acgcccggcc
     3241 agcgcaccag gaagggcgtg cgcagcgacg gttcgtacat ccagcgcttg tcgtaccagc
     3301 cgtgctcgcc caggtaccag ccctggtccg acatgtagac gacgatcgtg ttgtccgaca
     3361 gcccggcctc gtccaggtag tccagcatcc gcccgatgtt gtcgtcgagc gactgcaccg
     3421 accgcaggta gtccttcgcg tagcgctggt acttccagcg ggtgagggcc gaatccgaca
     3481 gggcggcggc gcggaccgtc tcgttgcgcg gcccgtagac ggcgtcccag gcccggcgct
     3541 gctccgcgtc gagctccgcc tgctccgcga tcttcaggtc gtgcgccgac aggttccgcg
     3601 cgacgctcat cgtcgcctcc gcggcggcgc gcgtgcggcc ggagtagtcg tcgaagaacg
     3661 tcgccggcat gggcacgtcc acgtcctcgt agtccgccag gtggtccggg cccggctgcc
     3721 agttgcggtg cggcgccttg tgctggaaca tgagcaggaa gggccgcgag gggtctcgtc
     3781 catcgcgcag ccactcgagg gcgatgtccg tgacgagatc ggtcgtgtac cccgggacgc
     3841 gcacggtgtc gccctgcgag ccgatcggcg gattgtagta cgggcccggt ccgatgagca
     3901 cccgccagaa gtcgaagccg gtcggggcgc tcttcaggtg ccacttgccg acgagcgccg
     3961 tctcgtaccc cgcctgctgc aggagcttcg ggaacgtctc ctgcgagccg tcgaacgttt
     4021 cggagttcgt cagctggccg ttgacgtggt tgtagcggcc cgtcaggacc gtcgcccggc
     4081 tcggcgcgca gatcgagttc gtgacgagcg cgttggtgaa gagcgccccc tcacgcgcga
     4141 tccggtcgat gttcggcgtc tcgttgatca ccgagccgta ggcgccgacc gcctggcgac
     4201 cgtggtcgtc cgagaagatg aagacgatgt tgggcggggg agccgcctcg cggcaggaga
     4261 gcgccgcgag ggccaggaga acgggcagga atcgacgcat gcggatcgtt cggttatctt
     4321 ggcgcaccat gcaggcagcg acatgagcag cgatcagcga ttcgacgacc ttgccgtgct
     4381 gtacgtgggg ctggctcacg gcatcgacgg ccgactgcac ttccaggaac ttacgcagat
     4441 cgagagccac ctggaacgct ggcggcatcc cggagagcgg cgcaacgtgt cttccgtggt
     4501 ggacgaggtg gtgcgagcgt acgatccgat ccgcgtcatc gacgccgtac agcgcctcgg
     4561 caggcagctc tccgaacggg agcagcgcgc ggcgctcgac gatctctcgg cgatcgcgct
     4621 cgcggaccgg cgcttcctga aggaggaggc cgagtacatc gggcgggtgg cgttcgactg
     4681 gggcctgcag ctcgacgaaa cggaggaggg cgcgctgtgg accgtgctgc gcagccgcgg
     4741 cgacgcggac ccgcgcgcgc tggccatgga tctcgcgctc ctgtacctgg ccgtggcgca
     4801 tcgcccggac gacgacattt cggaccggga gctcgccgcc atccgcggcg ccatggccgg
     4861 gtggctgccc ggtctgggcg acgagcaggt agagcggctg gtgcgttccg cgctcgctgc
     4921 cttcgcgcgt gggatggccg agggtggggc ggagatgatc gtccgccggg tcagggccac
     4981 gctcccgatc caccagcggc aggccgtggt gcgcgacatc cggtcgatcg ccggggcgga
     5041 cggagatccg acggtcgagg ccggccttct cgtgcactgg ctcgaggacg agctcgaggc
     5101 gcggtagaag ggcccgctgc gcacggccgg gcacgtgacg gacgcccgca tgccggaaaa
     5161 agtgcccgca actccgcgca aacccccttc gctgccagcg aaaagcagta tacgggattc
     5221 cccgacagcg ggtttggtca ccggtcgaag aggggcgtcc accggtcgcg aatcctcgat
     5281 gagccgcccg cggaacctgc ccggtgagcg cgaggcccct acgctgtagg gaatcggacc
     5341 acagcattgt cgcgagtctg cctctactcg ccagactgcg tgcgtcctaa taagcgcaac
     5401 caacgcaatc cgcccgttcg tgggctttcc ccggaccagg gcatcgcagc aaccattcac
     5461 gaatcgcagg ggacggcatc catggatacg cgaatacaac gcttcttcct gggattcctc
     5521 gcggccttcc tgctggttcc tgtcgcctcg gcgcaggacg gcaaggtgac cggcacggtc
     5581 accgatcggg cgaccggcga ggcactgcca ggggccacag tg
//
LOCUS       DBNHQZ010000046         3399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2043975, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000046 DBNHQZ010000000
VERSION     DBNHQZ010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2043975"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16YU_01160"
     CDS             <1..285
                     /locus_tag="AC16YU_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6063858.1"
                     /translation="TVGNFAAFFEIAAAARLDGTYGRIQLLPLVLMGFLVSVVASSRA
                     VVRQLFRHRRSPFEWDKTDRYRTAAPPEFEPNNRNSNHDAAGTAGPREKA"
     gene            282..1289
                     /locus_tag="AC16YU_01165"
     CDS             282..1289
                     /locus_tag="AC16YU_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6063859.1"
                     /translation="MSVLVLILIVAGTLLLVGLPLLPSIRTLRSGLDRDALVIQDWYD
                     DERHRTAADVDRRLRLSEAPEIEHLEGDFQLDRNEVLRSTVSVGGSFDAAADSTVGAV
                     HAGDEVRLGERVKATGWLHAGHAARMASGASVTGSLSAADTLDVAVGARFERLAAPTV
                     RFGRPAVPGSEEAAFLVQEPTPVPESVRWIAWSKAIYDDFEVLSRTWMNGDLIVRGEL
                     SVGTGAVIDGTVKVHGSIDVADGVRITGGLFCDGSVRLGKGCKIDGVCTAGSDMQIDS
                     DTVIGHEDRRVTVTAARMTVADGVRIHGVVVATESGSVEAELPPAPGRKARHPSTDPS
                     T"
     gene            1289..2479
                     /gene="wecB"
                     /locus_tag="AC16YU_01170"
     CDS             1289..2479
                     /gene="wecB"
                     /locus_tag="AC16YU_01170"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR6063860.1"
                     /translation="MSAKRLLLCMGTRPEIIKMGPVYHALKAAGMQPVLLHTGQHEEM
                     AWPLYEFFDMAPDHVVHLERERKSLGHVGGRMMDEIDRVMIETRTEAVLVHGDTTSAL
                     MGAMAAFQNRIPVGHVEAGLRSHDPMDPFPEEMNRTVIARIAHWHFAPTELAVQNLKR
                     ENVAWGRIFQVGNTVIDATHMAMARALEQYSPGGPRENCPAAPFVRKASGGRLVLVTL
                     HRRENWGEPIERVARTIRHLVESEPDIHLVWPVHLNPIVRESVFRVMGSLAPEVQERV
                     RLTVPLDYAQMLWILRHSWLVVTDSGGIQEEAAALGRPVLVARRTTERPELIQAGGGV
                     LVGTDPEVIRSWVSALSADPSAYERMSSIENPYGDGRSGERIARILREQLEVREDKTD
                     AAAA"
     gene            2463..3299
                     /locus_tag="AC16YU_01175"
     CDS             2463..3299
                     /locus_tag="AC16YU_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HIR6063861.1"
                     /translation="MQPQPDVTLRHLSYRFAATASALLLAASAAPAALSQDVLPPARN
                     MVEFGTMLHRYSDGIDPWNGIYLRTQIASGARNLWLGEVVAEDRLGEQAFAYKVEYVL
                     DQPRWFARAGFRTSTSGIYNPEWRTELRLGVKTGPERRLLLMGSGFHQKIRDGHRDSG
                     FAAEVQYYGGPAWIVQGAARWTYSTPGDVWSRYFDAALTLGRYGTRNVTLHGAFGTEA
                     YQIIGPLDILVDFESWMARLVWREWLSRKVGFTVSGIWYENDYYTRGGVEAGLFIHFG
                     DR"
BASE COUNT          519 a         1154 c         1211 g          515 t
ORIGIN      
        1 acggtgggca atttcgcggc cttcttcgag atcgcggcgg cggcccgcct cgacgggacg
       61 tacggtcgca tccagctgct cccgctggtg ctcatgggct tcctggtcag cgtcgtcgct
      121 tcgtcgcgcg ccgtggtccg gcagctcttc cgccaccgca gatcgccctt cgagtgggac
      181 aagacggacc ggtatcgcac ggcggccccg cccgaattcg agccgaacaa caggaattcc
      241 aatcacgatg cggccggcac tgccggtccg cgtgagaagg catgagcgtc ctcgtcctca
      301 tcctgatcgt ggccgggacg ctgctcctcg tggggcttcc gctgctaccg tccatccgga
      361 ccctgcgctc gggcctggat cgcgacgcgc tcgtcatcca ggactggtat gacgacgagc
      421 ggcaccggac ggccgccgac gtggatcggc ggcttcgtct ctcggaggca cctgagatcg
      481 agcacctcga gggcgacttc cagttggacc ggaacgaggt cctgaggtcc accgtgtcgg
      541 tcggcggcag cttcgacgcg gccgcggact ccaccgtggg cgccgttcac gcgggcgacg
      601 aggtccgcct cggcgagcgc gtcaaggcca ccggctggct ccacgcgggc cacgcggcgc
      661 ggatggcgtc cggcgcctcg gtgacgggca gcctctccgc cgcggatacc ctggacgtgg
      721 ccgtcggcgc acgcttcgag cgcctggccg ccccgaccgt ccgcttcggt cgaccggccg
      781 tacccgggtc cgaggaggcg gccttcctgg tgcaggagcc gaccccggtt ccggagagcg
      841 tgcgctggat cgcctggtcc aaggcgatct acgacgattt cgaagtgctg agccgcacct
      901 ggatgaacgg tgacctgatc gtgcgcggcg aactgagcgt cgggaccggc gcggtgatcg
      961 acgggacggt caaggtccat ggctcgatcg atgtcgccga cggcgtccgc ataacgggcg
     1021 gactgttctg cgacggaagc gtgcgcctgg gcaagggctg caagatagac ggcgtgtgca
     1081 ccgccggctc ggacatgcag atagattccg ataccgtgat cggtcacgag gaccgccgcg
     1141 tgacggtgac cgccgcgcgg atgacggtgg cggacggcgt gcggattcac ggcgtggtcg
     1201 tggccacgga gtcgggctcg gtcgaggccg agcttccgcc ggccccggga cggaaggcga
     1261 ggcatccctc aacggacccg agtacatgat gagcgcgaag cgacttctcc tctgcatggg
     1321 caccaggccg gagatcatca agatgggtcc ggtctaccat gcgttgaagg cggccggcat
     1381 gcagccggtc ctgctccaca cggggcagca cgaagagatg gcctggcccc tgtacgagtt
     1441 cttcgacatg gcgcccgacc acgtggtgca cctggagagg gagcgcaagt ccctcgggca
     1501 cgtgggtggc cgcatgatgg acgagatcga ccgcgtgatg atcgagacgc ggaccgaggc
     1561 cgtcctggtg cacggcgaca cgacctccgc gctcatggga gcgatggcag cgttccagaa
     1621 ccggatcccg gtcggccacg tggaggccgg cctgcgctcc cacgacccga tggatccgtt
     1681 cccggaggag atgaaccgca cggtgatcgc gcggatcgcc cactggcact tcgcgccgac
     1741 cgagctcgcg gtccagaacc tgaagcggga gaacgtggcc tggggacgca tcttccaggt
     1801 cggcaatacc gtgatcgacg cgacgcacat ggccatggcg cgcgcgctcg agcagtactc
     1861 gccgggcggc cccagggaga actgcccggc ggcgccgttc gtgcggaagg cgtccggcgg
     1921 ccggctggtg ctcgtgaccc tgcaccgccg cgagaactgg ggcgagccga tcgagcgcgt
     1981 ggcgcgcacg atccgccacc tggtcgagtc ggagcccgac atacacctcg tgtggccggt
     2041 gcatctgaac ccgatcgtgc gcgagtccgt gttccgggtg atgggcagcc tggccccgga
     2101 agtccaggag cgggttcgcc tgacggtccc gctggactac gcccagatgc tctggatcct
     2161 gcgccattcg tggctcgtgg tgacggactc gggcggcatc caggaggagg cggcggccct
     2221 gggccggccg gtcctcgtgg cccgtcgtac gaccgagcgc cccgagctca tccaggcggg
     2281 tggcggcgtg ctggtcggca ccgatccgga ggtcatccgg agctgggtca gcgccctgag
     2341 tgccgatccg tcggcctacg agcgcatgag ttcgatcgag aacccgtacg gggacggccg
     2401 gtccggcgag cggatcgccc gcatcctgcg cgagcagctc gaggtccgcg aagacaagac
     2461 cgatgcagcc gcagcctgac gtgacgctcc gccacctgtc atatcgtttc gccgcgacgg
     2521 cctcagcgct cctcctggcg gcctcggcgg cgccggcggc cctgtcgcag gacgtgcttc
     2581 cgccggcccg caacatggtc gaattcggca cgatgctgca ccggtactcc gacggcatcg
     2641 atccgtggaa cggcatctac ctgcgcacgc agatcgcatc gggcgcccgg aacctgtggc
     2701 tcggcgaggt ggtggccgag gaccgcctcg gcgagcaggc cttcgcctac aaggtggagt
     2761 acgtcctgga ccagccccgc tggttcgccc gcgcgggctt ccggacgtcc acgagcggca
     2821 tctacaatcc cgagtggcgc acggaactgc ggctcggggt gaagaccggc ccggagcggc
     2881 ggctgctgct catgggctcc gggttccacc agaagatccg ggacggccac agggacagcg
     2941 gattcgccgc ggaggtccag tactacggcg gtcccgcctg gatcgtgcag ggagccgccc
     3001 gctggacgta cagcacgccg ggcgacgtgt ggtcgcgcta tttcgacgcg gcgctgacgc
     3061 tcggccgcta cggcacgcgg aacgtcacgc tccacggcgc cttcggcacg gaggcctacc
     3121 agatcatcgg tccgctggac atcctggtcg acttcgagag ctggatggcg cgcctggtct
     3181 ggcgtgagtg gctgagccgc aaggtcggct tcaccgtgtc cggcatctgg tacgagaacg
     3241 actactacac ccgcggcggc gtcgaggcgg gcctcttcat ccacttcggc gaccgatgag
     3301 cgcaccggct tcccatagcg accggggata cgcccgccgg gcgaccacgt acctgcacct
     3361 gcatcggggc atccagcggg aggaggcgac ctcgcatcc
//
LOCUS       DBNHQZ010000047         4969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3535195, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000047 DBNHQZ010000000
VERSION     DBNHQZ010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3535195"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="AC16YU_01180"
     CDS             complement(<1..954)
                     /locus_tag="AC16YU_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HIR6063862.1"
                     /translation="MPRLAHGLLLLLLFALIDVARAQEARLLRQPDLSESHIAFTYGS
                     DVWIVGRQGGEARRITSTPAVESDPHFSPDGRWLAFTSNRSGIDAVYVVPIEGGAPTR
                     LTWYPASSRARGWTPDGHVLYSSSRETAPGGYSRLWTVAPDGGPSVRIPAPRGNDASY
                     AQDGRRLAIEPISRWDVEWRGYRGGQNQPLVILDLETLGERRIPGDLTTDVQPLWLGD
                     TIYFLSDRDWAMNVWAYDPATEALRQVTRFTGAEAKWLAGHDGTLVIEQDGWIHTVDP
                     ATGRASRIPIIVRGDFPWMEPRWEDVSGRAGSASLSPTGQRI"
     gene            complement(999..1511)
                     /locus_tag="AC16YU_01185"
     CDS             complement(999..1511)
                     /locus_tag="AC16YU_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6063863.1"
                     /translation="MNDTAERLSNTVRVAAERLLAMPDEAARRRPAPGKWSPIEIIGH
                     LVDSASNNHQRLVRALFRTDFVFEGYDADDWVDSQHYREAPWPALVVLWREFNLHLAR
                     VVDSTPDEKLREPKMPHALHRIAWQKPPENEPVTLSWWFADYVGHLEHHLRQIPLDLG
                     VAVSAPSPRP"
     gene            1792..4488
                     /locus_tag="AC16YU_01190"
     CDS             1792..4488
                     /locus_tag="AC16YU_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6063864.1"
                     /translation="MKHLPLAVLALSTVLFPAQAVAQATLRGVVTDQETDEPLIGANV
                     VVIGTTNGTTTGIDGAFEFVIARPLPVLIRVTYVGYAAAEVEVAAAGAALSIELEPRI
                     LSQGSVVVSASRKSEKITEAPAAVSVLFGRDLEGQTTTSPAEVLRNVVGVDVSEQGIN
                     SYTVTLRGRNLAFDTATHILIDNRQATTPGLAVTIFGRQPLNPDDLERVEVIRGPGSA
                     LYGPGVDAGVIHFITKSPFDHPGTTVSVAGGEQSSFRITGTHRGVAGPKLGYKVTGMY
                     SRADNWPYDPNDPEDRAILEIIPDTILSGVDGRLFDVIDGLDEETFSYAATGSVEYRL
                     DPSTSLTADAGYTWYKGINRFTLGEIFNEFPEMYGQLRLSRGNLFVQANYLNAANDER
                     TFLYRTGRTLAFNNRKYQLQAQHAARVPSIRTSLTYGGEYQMVTTDTKGTINGMFEED
                     DETVQFGTYLQSQSELTPRLSLTLAGRVDRFQTIERTVFSPRAGLVFQPWPGHSIRLT
                     FNRAFAAPTNLSLYTDVAVADLSEADPAFADLEGIGDVRLVGGSVPITFDEPPVTVSF
                     IPGVDPYDGIGMPLGAAYRVALRGMSGSLPPELLAFLESGADRVSGFSAGIPLDVQDG
                     FGVIRERDYLLKPRRTDMVELGYTGVSPRLYVSASAYYTWKRDFESDLRIFSPLVIGP
                     SLGEDLRDAVDAAFTDEELAAADLDRSTLLAWFEEAGGALSEFPVGLLQPDQVPVTGR
                     PQLVGSFVNYGEIEYLGVELSATALLADRVSATANFTGLSQIDFMGEDLGLDRASMDR
                     YALNVPKYSYGLKVDYGTGAEGLFASAAFRHKGAFEGDFGDLFPGEVDEQNIVDLALG
                     YGLANGVRFNVTAQNVLDDTYRSFPGLPKIGRLVLASVRYSF"
     gene            complement(4547..4768)
                     /gene="infA"
                     /locus_tag="AC16YU_01195"
     CDS             complement(4547..4768)
                     /gene="infA"
                     /locus_tag="AC16YU_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6063865.1"
                     /translation="MAKQEPIKQEGVVTEALPNAQFRVELTNGHVVLGLLSGKMRKFF
                     IRILPGDRVDIEMSPYDMTKGRIVYRYKT"
BASE COUNT          797 a         1640 c         1732 g          800 t
ORIGIN      
        1 gatgcgctgc ccggtgggcg agagggatgc ggagccggcc cggccgctga cgtcctccca
       61 gcggggctcc atccacggga agtccccgcg gacgatgatc gggatccgcg aagcccggcc
      121 ggtcgccgga tcgacggtgt ggatccatcc gtcctgctcg atgaccaggg tcccgtcgtg
      181 gccggcgagc cacttggcct cggcgccggt gaatcgcgtg acctggcgca gggcctccgt
      241 ggcgggatcg tatgcccaga cgttcatggc ccagtcgcgg tccgacagga agtagatggt
      301 gtccccgagc cagagcggct ggacgtccgt ggtcaggtcg cccggaatgc gccgctctcc
      361 gagggtctcg aggtccagga tcacgagggg ctggttctgg cctccccggt agccccgcca
      421 ctccacgtcc cagcgggaga tcggctcgat cgccaggcgg cgtccgtcct gggcgtagga
      481 ggcgtcgttg ccccgcgggg cggggatcct gacggacggc ccgccgtcgg gggccaccgt
      541 ccacagccgg gagtagcccc ccggagccgt ttcccgcgac gaggaataca ggacgtgccc
      601 gtcgggggtc cagccccggg cccgggacga cgcggggtac caggtgagcc gggtcggcgc
      661 tccgccctcg atcggcacca cgtacaccgc gtcgatgccg gagcggttcg aggtgaacgc
      721 gagccatcgg ccgtcgggcg agaagtgcgg gtcgctctcg acggccgggg tcgacgtgat
      781 gcggcgcgcc tccccgccct gccgccccac gatccagacg tcggagccgt aggtgaacgc
      841 gatgtgcgac tcgctcaggt cgggctgccg gagcagccgt gcctcctgcg cgcgcgcgac
      901 gtcgatgagg gcgaaaagga gcaggaggag gagtccgtgg gcgaggcgcg gcatgacagg
      961 tgactgcgga tggagtgaat tcggacggga agcagggatc agggccgggg ggacggggcg
     1021 gacacggcga cgcccagatc cagcggaatc tgccggaggt ggtgctcgag gtgcccgacg
     1081 taatcggcga accaccacga gagggtcacc ggttcgttct cgggcggctt ctgccaggcg
     1141 atgcggtgca gggcgtgggg catcttcggc tcccggagct tctcgtccgg cgtcgagtcg
     1201 acgacccggg ccaggtgcag gttgaactcc cgccacagca cgacgagcgc cggccacggg
     1261 gcctcgcggt aatgctggga gtccacccag tcgtccgcgt cgtagccctc gaacacgaaa
     1321 tcggtccgga acagggcgcg caccaggcgc tggtggttgt tcgaagcgga gtccacgagg
     1381 tgcccgatga tttctatcgg ggaccatttc cccggcgccg ggcgtcgtcg agcggcttcg
     1441 tcgggcatgg cgaggagccg ctcggcggcg acccggacgg tgttgctcag tcgttccgcg
     1501 gtgtcgttca tccttcgtct ccccttccgt gcttgacgat gagacccgcc ggccatgcgg
     1561 ccgacccggg gtccctatct attcaacgac cgggcgatcc gcaagcccgc tcggcaagag
     1621 ggcccggggt cgtgggaagt gtcccgcccg tcatgcagga atgtcgggat tggcccggcg
     1681 ggcgcgtcgt ctattgcggg agtccacccc ctgtcaacca ggcatgccgg tttcgtacca
     1741 ttcggtccaa tccgttgcga tccggtccac gtgccccagg tacccccggt catgaagcac
     1801 cttccccttg ccgtcctcgc cctctcgacc gtcctgttcc cggcgcaggc cgtcgctcag
     1861 gccacccttc gcggcgtggt aacggaccag gagacggatg aaccgctcat cggggcgaat
     1921 gtggtcgtca tcgggaccac gaacggcacc acgacgggaa tcgacggagc attcgagttc
     1981 gtcatcgcgc ggcccctccc cgtactgatc cgggtgacct acgtcggtta cgcggcggcc
     2041 gaagtggagg tcgcggcggc gggcgcggcg ctctccatcg agctcgagcc acgcatcctg
     2101 tcgcagggat ccgtcgtggt gtcggcatcc aggaagtcgg agaagatcac ggaggcgccc
     2161 gctgcagtct cggtcctctt cggacgggac ctcgagggcc agaccacgac cagtcccgcg
     2221 gaagtgctgc gcaacgtggt cggcgtggac gtgagcgagc agggcatcaa cagctacacg
     2281 gtgacgctac ggggtcgcaa cctggcgttc gacaccgcca cgcacatcct gatcgacaac
     2341 cggcaggcga cgacgccggg cctggcggtc accattttcg ggcggcagcc actcaacccc
     2401 gacgacctgg aacgcgtcga ggtcatccgc ggacccggat cggcgctgta cgggcccggc
     2461 gtggacgcgg gcgtgatcca tttcatcacg aagagcccct tcgaccaccc gggaacgacc
     2521 gtcagcgtgg cgggaggcga gcagtcatcg ttccggatca cgggcacgca ccggggcgtg
     2581 gccgggccga agctggggta caaggtcacc ggcatgtact cgcgcgccga caactggccg
     2641 tacgatccga acgatcccga agacagggca atcctcgaga tcatcccgga cacgatactg
     2701 agcggggtgg acgggcggct attcgacgtg atcgacgggc tggacgagga gaccttcagc
     2761 tacgccgcga cgggatcggt cgagtaccgt ctcgatccgt cgaccagcct gaccgccgac
     2821 gcggggtata cctggtacaa gggcatcaac cggttcacgc tcggggagat cttcaacgag
     2881 ttccccgaga tgtacgggca gctgcggctg agcaggggca acctgttcgt gcaggccaac
     2941 tacctgaacg cggcgaacga cgagaggacc ttcctgtacc ggacgggccg gaccctggcg
     3001 ttcaacaacc gcaagtacca gctccaggcg cagcacgcgg cccgggttcc gtcgattcgc
     3061 acgagcctga cctacggcgg cgaataccag atggtcacga ccgacacgaa gggcacgatc
     3121 aacgggatgt tcgaggagga cgacgagacg gtccagttcg gaacgtacct gcagtcccag
     3181 tcggagctca cgcccaggct ctccctgacg ctcgccgggc gggtggaccg attccagacc
     3241 atcgagcgca cggtgttctc gccgcgcgcg ggtctcgtat tccagccctg gcccgggcat
     3301 tcgatccgcc tgacgttcaa ccgggcgttc gccgctccga cgaacctctc gctctacacg
     3361 gacgtggcgg tggcggacct ttccgaagcc gacccggcgt tcgccgatct cgaggggatc
     3421 ggggacgtac ggctggtggg cggctccgtc ccgatcacgt tcgacgagcc gcccgtgacc
     3481 gtgtccttca ttccgggcgt cgatccgtac gacggcatcg gcatgccgct gggcgcggcg
     3541 tatcgggtgg cgctccgcgg gatgagcgga agtctccctc ccgaactcct ggccttcctg
     3601 gaatccgggg cggaccgggt ctccgggttc tccgcgggaa ttccgctcga cgtccaggat
     3661 ggcttcggcg tcatacggga gcgggattac ctgctgaagc cgcgccggac ggacatggtc
     3721 gaactggggt acacgggggt ctcgccgcgc ctgtacgtca gcgcgtccgc gtattacacc
     3781 tggaagcggg acttcgagtc cgatctccgg atcttcagcc cgctggtgat cgggccgagc
     3841 ctgggggagg atctccggga cgccgtggac gccgcgttca ccgacgagga gctcgcggcc
     3901 gcggatctgg accgttcaac gctcctggcg tggttcgagg aggccggggg cgccctttcg
     3961 gagtttccgg tcgggctgct tcaacccgac caggtgccgg tgacgggacg tccgcaactc
     4021 gtcggcagct tcgtcaacta cggcgagatc gagtacctgg gcgtcgaact gtcggccacc
     4081 gcgctcctcg cggaccgcgt gagcgcgacg gcgaacttca ccgggctcag ccagatcgac
     4141 ttcatgggag aggacctggg gctcgaccgc gcgagcatgg atcgatatgc cctgaacgtg
     4201 ccgaagtaca gctacggcct gaaggtggac tacgggaccg gcgccgaggg gctgttcgcg
     4261 tccgccgcgt tccgtcacaa gggcgccttc gagggcgact tcggggatct ctttcccggc
     4321 gaggtcgacg aacagaacat cgtggacctg gccctgggat acggtctcgc gaacggagtc
     4381 cgcttcaacg tgacggccca gaacgtgctc gacgacacct accgcagctt cccgggcctg
     4441 ccgaagatcg gccgcctggt gctggcatcg gtgcgctact cgttctgagg cgcgtcctcc
     4501 ggccgaagcg caggccaggc ccgcgcatcc ggccgatcgg cggacgtcag gtcttgtagc
     4561 ggtagacgat ccgtcccttg gtcatgtcgt agggggacat ctcgatgtcc acccggtcac
     4621 ccggaagaat gcggatgaag aacttgcgca tcttccccga gagcagtccc agcaccacgt
     4681 gtccgttggt cagctcgacg cggaactggg cattgggcag tgcctcggtg acgactcctt
     4741 cctgttttat cggttcctgc ttggccatgc acgctgaacg ttcgtgaaag acggtcccgc
     4801 gaacctgcct gatgccgggt ccggaccgta cagttcatgt atacgcccgg taacgcacaa
     4861 agggttctcc gcgggtcatt ccgcgagccg gggcagctct tcgatgcgct cctggatgtc
     4921 cagcggcatg gccccgcccg acttcagcgc gatctcgtac aggaagcgg
//
LOCUS       DBNHQZ010000048         5319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2586478, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000048 DBNHQZ010000000
VERSION     DBNHQZ010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2586478"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="AC16YU_01200"
     CDS             <1..1899
                     /locus_tag="AC16YU_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6063866.1"
                     /translation="NYQASLSGPVPLVPSTTFLVNGQRIVDMGHLMGERRFLPTDRND
                     FERFIFNPSGDGAIVPMNFSKQWSWLGKLTNRSLKNVQLGYQVVGSLSESRGYNHAYR
                     FNPDGQKTARRFSIVHGLDVTHTLSDRTFYELNLRQNYIDYEDLKYEDLGTAPEGAGT
                     RYALPEDSPYYVPGQPRGDANYEEGAIVQGVDLGRFVQSTNALVVKASVTSQATRTHL
                     LKGGIELQVADLRFGNPRRIDQVVIDGIQQLGVVQDTLGAVVYKYDPVNAAAFLQDRV
                     EWGDIRVRAGVRLEYFDANSTVPSNLQNPANSIDGAPESRPKATTAKLVVAPRLGISF
                     PILETASVFFSYGHFYQMPGLGQMFSNADYSILEDLQAGAVSYGVLGNPDLRPEFTSQ
                     YEFGFKSEITSFLGLDVSLFYKDIRDLLGVEFVQTYTAAEYARLTNIDFGGVRGFTIS
                     LDQRRVGPISTSLDFTLQTAIGNASDPRETANRAAAGEDPRPRQIPFNWDQRNTLNGT
                     VVWLEPGNFSVTTVVRFATGQPYTPSISTGFGADLEANSGRKPSFATVDLRAEKFVSM
                     GGVEWTVFARVFNLFDQHFTNGFVYSDTGSPYYTVVPTQQRNPNPGRLSEPRRIEIGL
                     SLRGAITK"
     gene            1907..5203
                     /locus_tag="AC16YU_01205"
     CDS             1907..5203
                     /locus_tag="AC16YU_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR6063867.1"
                     /translation="MIKRLTALGAFVLLALPAGAQELQPVVPPELRGSIDAERVGVHD
                     ANRVRTMFYNFGMVGDYVTNPDLSVFHSVEVPKGSGMNYSDGITPFVLARIVQENGRT
                     AEIMETGFRERQASSPISGRVMRFEPRPGYNEPNPDINKGRSIAISNDPRTWPGAADE
                     SGEIIQGAMPDDCWIDKVNDPDDPGWCGSWNGFFGKRPNADQESFSVMDDQFYDAWNF
                     YPDSRDRTRRGLGLRVEVRGFQWANPQAQNVIFWHYDIVNESTTDYDDIVFGLYMDSG
                     VGGAAISCDGTAESDDDNAFYDKSFGLNLVYTWDKGGRGVSLSSNCMRTGYLGYAYLE
                     TPGDSFNGLDDDDDGIVDEVRDGGPGQRIDGQDAIRSWVSANYDLAAFENAYGPLIER
                     PAYVAGVWWTGDEDMDWTAEFNDTGADGIFEDEDNPADTGEMDGMPTAGESNFDQTDI
                     NESDMIGLTGFKLNRIRAGAGAPSSETDNIVFYMNEREWPYRLYEQFTSPDENVRFDA
                     PLALDYNIGFLFASGPFRLEAGRRERFSLALAYGEDLIELESTVGVVQSIYNANYRFA
                     TPPPLPTLKAHAGDGFVTLTWDNKAERSIDPVTNRNDFEGYRIYRSTDPNFLDPQVIT
                     NARGTGPLGNGRPLVQYDLENGIVGFSDIVVQGTAYWLGQDTGITHTFTDSSVVNGQT
                     YYYAVTSYDHGSDEFGFFPSENAITISRTPRGGTILPSNTVEVRPNAPVPGFIPASVD
                     AESFEQVSGRGTGSVDAVVVAPGLVPDGHTLRLEFESLADSIRALTYSLWDATTGERL
                     FDGGKDLEGEGTGPSAAGVLPVVSTLATVEVDSLLTGFTDGSGTNAVYQARYASPVPI
                     NVRRPGFPEDLVLTFADTPLDTSQAAIGAPAIPANFRITGVDSGTQFDFRFRDVNGDR
                     VVSAAGEFIEILMEESLSSTRRRPVWRIELDATRGPATVTPPRSGDVYELVLAQPYGD
                     EDAFTFRVWGESVDLNAAREQLDRQEPYVVPNPYVASAQFEPERFAVSGRGVRRIEFR
                     GIPLDATIRIYTLRGDLVQTLRHDGTTTGMVPWNLRSRDNLEVAPGLYLFHVESDGLE
                     DFVGKFAIIK"
BASE COUNT          937 a         1784 c         1749 g          849 t
ORIGIN      
        1 aactaccagg cgagcctgag cgggccggtc ccgctcgtcc cgtccacgac gttcctggtg
       61 aacgggcagc gcatcgtcga catggggcac ctgatgggag agaggcggtt cctcccgacg
      121 gaccggaacg acttcgagcg gttcatcttc aatccgtcgg gtgacggcgc catcgtgccg
      181 atgaacttct cgaagcagtg gtcgtggctg gggaagctca ccaaccgctc gctcaagaac
      241 gtgcaactgg gataccaggt ggtcggcagc ctctccgaga gccgggggta caaccacgca
      301 taccggttca acccggacgg ccagaagacg gccaggcgct tctcgatcgt gcacggactc
      361 gacgtcacgc acacgctctc cgacaggacg ttttacgagc tgaaccttcg gcagaactac
      421 atcgactacg aggacctgaa gtacgaagat ctcgggaccg cgccggaggg ggccgggacc
      481 cggtacgcgc tgcccgagga ctcgccgtac tacgtccctg gacagccccg cggcgacgcc
      541 aactacgagg agggggccat cgtgcagggc gtcgatctgg gccgcttcgt ccagagcacc
      601 aacgcgctgg tcgtgaaggc gtccgtcacc tcgcaggcca cccgcacgca cctcctgaag
      661 ggcggcattg agctgcaggt ggccgacctg cggttcggca acccgaggcg catcgaccag
      721 gtggtcatcg acggcatcca gcagctcggc gtggtgcagg acacgctcgg agcggtcgtc
      781 tacaagtacg acccggtgaa cgccgcggcc ttcctgcagg accgggtgga gtggggggac
      841 atccgggtca gggccggcgt gcgtctcgag tacttcgacg cgaactcgac ggtgccctcc
      901 aacctccaga atcccgccaa ctcgatcgac ggcgccccgg agtcccgccc gaaggccacg
      961 acggccaaac tggtcgtggc cccgcgcctc ggcatctcgt tcccgatcct ggagacggcg
     1021 tccgtcttct tctcgtacgg gcacttctac cagatgccgg ggctcggcca gatgttctcg
     1081 aacgccgact attcgatcct ggaggatctg caggccggcg ccgtttcgta cggcgtgctg
     1141 ggcaatcccg acctccggcc cgagttcacc tcgcagtacg agttcgggtt caagtccgag
     1201 atcacgagct ttctcggact cgacgtgagc ctcttctaca aggacatccg tgacctgctc
     1261 ggcgtcgagt tcgtgcagac gtacacggcg gcggagtacg cgcgcctcac gaacatcgac
     1321 ttcggcggcg tgcgcgggtt cacgatttcg ctggaccagc ggcgcgtggg gccgatctcc
     1381 acgtcgctcg acttcaccct gcagacggcc atcggcaacg cctccgatcc gcgagagacg
     1441 gccaaccggg ccgcggctgg agaggaccct cggccgcgcc agatcccgtt caactgggac
     1501 cagagaaaca cgctgaacgg cacggtggtg tggctcgagc ccggcaattt ctcggtcacg
     1561 accgtcgtgc ggttcgcgac cgggcagccc tacaccccgt cgatctcgac cgggttcggt
     1621 gcggacctcg aggcgaattc cgggcgcaag ccctcgttcg cgacggtcga cctgcgggcc
     1681 gagaagttcg tgtcgatggg cggcgtcgag tggaccgtct tcgcgcgggt cttcaacctg
     1741 ttcgaccagc acttcaccaa cggcttcgtc tactccgaca ccggcagtcc ctactacacc
     1801 gtggttccga cgcagcagag aaacccgaat ccgggtcgcc tgagcgaacc ccgccggatc
     1861 gagatcggac tctccctccg gggtgccatc acgaagtaga atcccgatga tcaagcggct
     1921 cacagcactc ggtgcgttcg ttctcctcgc gttgccggcc ggcgcgcagg agctgcagcc
     1981 ggtcgttccg ccggaactcc ggggctcgat cgacgcggag cgggtgggcg tccacgacgc
     2041 caatcgcgtc cggaccatgt tctacaattt cgggatggtc ggggactacg tcacgaaccc
     2101 ggacctgagc gtcttccatt ccgtcgaggt gcccaaggga tcgggcatga actactcgga
     2161 cggcatcacg ccgttcgtgc tggcccgcat cgtacaggag aacggccgga cagcggagat
     2221 catggagacg ggattccggg agcgccaggc ctcgagcccg atctcgggcc gggtgatgcg
     2281 gttcgagccc cgcccgggct acaacgagcc gaaccccgac atcaacaagg gccgctccat
     2341 cgccatcagc aacgatccgc gtacgtggcc gggcgccgcg gacgagagcg gggagatcat
     2401 ccagggcgcg atgcccgacg actgctggat cgacaaggtg aacgacccgg acgacccggg
     2461 atggtgcggt tcgtggaacg ggttcttcgg gaagcggccc aacgcggacc aggagtcgtt
     2521 ctcggtcatg gacgaccagt tctacgacgc ctggaatttc tatcccgact cgcgtgaccg
     2581 gacgcggcgc gggctcgggc tgcgcgtgga ggtgcggggc ttccagtggg cgaacccgca
     2641 ggcccagaac gtcatcttct ggcactacga catcgtcaac gagtcgacga cggactatga
     2701 cgacatcgtg ttcggcctgt acatggactc cggcgtgggc ggcgccgcga tatcgtgcga
     2761 cggaaccgcc gagtcggacg acgacaacgc gttctacgac aagagcttcg gtctgaatct
     2821 cgtctatacc tgggacaagg gcgggcgcgg cgtatcgctc tcgagcaact gcatgcggac
     2881 gggctacctc gggtacgcct acctggagac gcccggcgac tcgttcaacg ggctcgacga
     2941 cgatgacgac ggcatcgtgg acgaggtccg cgacgggggt ccggggcaga ggatcgatgg
     3001 gcaggacgcg atccggtcct gggtttcggc caattacgat ctcgccgcct tcgagaacgc
     3061 gtacggaccc ctcatcgagc gtcccgccta cgtggccggc gtatggtgga ccggagacga
     3121 ggacatggac tggaccgcgg aattcaacga cacgggagca gacggcatct tcgaggacga
     3181 ggacaacccg gccgacaccg gggagatgga cgggatgccg accgccggcg agtcgaattt
     3241 cgaccagacg gacatcaacg agtccgacat gatcggactc acgggtttca agctgaaccg
     3301 catccgcgcc ggcgccggtg cgccgagctc cgagacggac aacatcgtct tttacatgaa
     3361 cgagcgcgaa tggccctacc ggctttacga gcagttcacg agccccgacg agaacgtccg
     3421 cttcgacgcc ccgctcgcgc tcgactacaa catcggtttc ttgttcgcct cgggcccctt
     3481 ccgtctcgag gcgggtcggc gcgagcgctt cagcctggcg ctggcctacg gggaggatct
     3541 gatcgagctc gagtccacgg tcggcgtcgt ccagtcgatc tacaacgcca actaccgctt
     3601 cgctacgccc ccgccgctgc ccacgctcaa ggcgcacgcc ggagacgggt tcgtgacgct
     3661 cacctgggac aacaaggccg aacgctccat cgacccggtc accaaccgga acgacttcga
     3721 aggctaccgg atctaccgct ccacggaccc gaacttcctg gacccgcagg tcatcacgaa
     3781 tgcgcgcggg acgggtcccc tgggcaacgg gcggcccctg gtccagtacg atctggagaa
     3841 cggcattgtg ggcttctcgg acatcgtggt gcagggcacg gcctactggc tgggacagga
     3901 caccgggatc acccacacgt tcacggactc aagcgtggtc aacggccaga cctactacta
     3961 cgccgtgacg tcgtatgacc acggttcgga cgaattcggg ttcttcccgt ccgagaacgc
     4021 gatcacgatc tcccgcaccc cgcggggcgg cacgatcctg cccagcaaca cggtggaggt
     4081 ccgtccgaac gcacccgttc cgggcttcat tcccgcgtcg gtcgatgccg agtctttcga
     4141 gcaggtatcc ggccgcggaa cgggctcggt ggacgcggtc gtcgtggctc ccgggctcgt
     4201 accggacggg cacacgctgc gcctggaatt cgagagcctg gcggacagca tccgtgcgct
     4261 gacctactcc ctgtgggacg cgacgaccgg cgagcgcctg tttgacggcg gcaaggacct
     4321 cgagggcgag ggcaccggtc cgtcggccgc tggcgtactg cccgtcgtgt cgacgctcgc
     4381 caccgtggag gtcgacagcc tgctgacggg cttcacggac gggagcggca cgaacgccgt
     4441 ctaccaggcg cgctatgcgt ctccggtccc catcaacgtc cgcaggcccg ggttcccgga
     4501 agacctggtc ctgaccttcg cagacacgcc gctcgacacg tcgcaggccg ccatcggggc
     4561 gccggcgatc ccggccaatt tccggatcac gggcgtggac tccggcacgc agttcgactt
     4621 ccggttccgc gacgtgaacg gcgaccgtgt cgtgagcgcg gccggagagt tcatcgagat
     4681 cctgatggag gagtcgctga gctccacccg ccgtcgcccc gtgtggcgga tcgagctcga
     4741 cgcgacccgc ggcccggcga ccgtgacgcc gccccgatcg ggcgacgttt acgaactggt
     4801 gctcgcccag ccctacggcg acgaggacgc gttcacgttc cgggtgtggg gcgagtcggt
     4861 cgacctgaac gcggcgcgcg agcaactgga ccgccaggag ccgtacgtgg tgcccaatcc
     4921 gtacgtggcc tctgcccagt tcgagccgga gcggttcgcc gtgtccggac ggggcgtgcg
     4981 gcggatcgaa ttccgcggca tccctcttga cgcaacgatc cgcatctaca cgctgcgggg
     5041 cgacctggtc cagacgctcc ggcacgacgg caccacgacc ggcatggtgc cgtggaacct
     5101 gcggtccagg gacaacctgg aggtggcgcc cgggctgtac ctgttccacg tcgagagtga
     5161 cggcctggaa gacttcgtgg gcaagttcgc catcatcaag tgacgtgaac ccatgccctc
     5221 ccacagtaca ggaagacggt gaaggagatg cgcctcgata ggactctgct gctggcggcg
     5281 atcctgtttg ccgccccgcc cgtcgtccac gggcagagc
//
LOCUS       DBNHQZ010000049         3643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3535934, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000049 DBNHQZ010000000
VERSION     DBNHQZ010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3535934"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..2182
                     /locus_tag="AC16YU_01210"
     CDS             80..2182
                     /locus_tag="AC16YU_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR6063868.1"
                     /translation="MRVRRQLPTLRAAAICAGLATLLGPAASLAVRAQGVRATIDAAE
                     THAPISHHLYGQFLEHIGDIVNDGIWAELLDDRKFYYPVVETEPERAPGRRPGPRRWT
                     TIGEPDRVAMDTQRPFVGRHSPKIVPGGDRPAGFGQAGLAVEAGESYVGRIALAATGD
                     VRVEVTLVWGPASGDRQIVSTAPWAADYFTYPLTFVPAGSTTDARLEITATGLGTVHV
                     GAVSLMPADNLGGFRKEVVEALRSLRSGAYRFPGGNFVSAHEWRNAIGDPDRRPPTWD
                     PVWRAVQPNDVGTDEFILLCRLLDVEPYITVNAGFGDAWSAAQLVEYVNGSTLTTMGR
                     LRAANGHPEPYDVRFWGIGNEAWGSWQMGAMSLDQFVLKHNEFAEAMRAVDPSIVLIA
                     SGAMPDAMTGSGESQKRTGKIVPEPLGPADWTGGLFLHSLDHMDWISEHYYSYGGTRY
                     DVTRGEQVPVDPDEPLVDWMRRPANHVRLKVEAYEDYEERIPALRDKPVPIVIDEWAF
                     AGGPIRPDSYRAVPAYAWAFHEMFRRTDLFVMANFTFATSMVSATRTEAVLNPTGLLF
                     KLYRDHFGTIPVTVTGNSPQSAPKYPVGGEEPNLNAGSPTFPLDVAAAWTEDRSALTI
                     AVVNPTESELPLELGISGAELAGTGTRWRMAPESLDATVSVGRNPEVVVEEETVAGTL
                     SSVTLPRFSVTIYRLEAR"
     gene            2188..>3643
                     /locus_tag="AC16YU_01215"
     CDS             2188..>3643
                     /locus_tag="AC16YU_01215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR6063869.1"
                     /translation="MQRGPACVLVAWLVLASLVPSAPALAQERARNPILWADVPDPSV
                     VRVDSVYYMTSTTMHMSPGVPVMRSTNLVDWTTVGYVYDELANADALFMRQGQDAYGR
                     GTWASSLRHRDGLFYVVTFSYTTGETYVFTTDAIERGPWRKASLGGLYHDPSLLFDDD
                     GRVYLVYGAGNIRIVELAADARAVKPGGEDRVLIPNAGGIAGSDFWVPGEGSHIVKVD
                     GRYYLFLIVWPAGGMRTELVYRADSLEGPWEGRIVLEDEGIAQGGVVDTPDGRWYAML
                     FGDRGAVGRIPYLVPVAWTDGWPVFGTDGEVPATLDIPSDGTGVRGIVASDEFDGTHL
                     GPAWQWNHQPDGDRWSLGARPGWLRIVNGRPGRSLVDARNTPTQRTFGPRSSATTLLD
                     AGGLQDGDAAGLAILMEHYAWIGVVREGGRASLVVTALQDGGETETARVPLDREVVYL
                     RVDADFRDLKDEATFSYSLDGKAWIPVGEPFEMRY"
BASE COUNT          553 a         1275 c         1270 g          545 t
ORIGIN      
        1 gtcacctttg cgtgcctctt cctccggatc cgcgagtcgg aactcacggt ccacctcttc
       61 gtcgcttccg tcaaccgcca tgcgagtccg ccgccaactc cccaccctcc gagctgcggc
      121 gatttgcgcc gggctcgcca cgcttctggg cccggccgcg tcgctcgcgg tacgggccca
      181 gggtgtccga gcgaccatcg atgcggccga aacccacgcg ccgatatcgc atcacctgta
      241 cggacagttc ctcgagcaca tcggcgacat cgtgaacgac ggcatctggg cggagctgct
      301 ggacgaccgc aagttctact accccgtggt cgagaccgag cccgaacgcg cacccggccg
      361 ccgtcccggc ccgcgacgct ggacgacgat cggcgagccg gaccgcgtcg ccatggacac
      421 gcagcgcccg ttcgtcggtc ggcacagtcc gaagatcgtg cccggaggcg accggccggc
      481 cggcttcggc caggcgggcc tcgcggtcga agcgggtgaa tcgtacgtcg ggcgcatcgc
      541 gctcgccgcg acgggtgacg tgcgggtcga ggtgaccctg gtctggggac ccgcatccgg
      601 ggaccggcag atcgtgtcga ccgccccttg ggccgcggac tacttcacct acccgttgac
      661 cttcgttccg gccgggagca cgaccgacgc ccggctcgag atcacggcca ccgggctcgg
      721 gacggtgcac gtgggcgccg tctcgctgat gcccgccgac aacctgggcg ggttccggaa
      781 ggaggtggtg gaggcgctcc ggtccctgcg ctcgggtgcg taccggttcc ccggcggcaa
      841 cttcgtgtcg gcgcacgaat ggcggaacgc gatcggagac ccggaccgcc ggccgccgac
      901 ctgggatccg gtctggcgcg ccgtgcagcc caacgacgtg ggcacggacg aattcatcct
      961 gctgtgccgt ctgctggacg tcgagcccta catcacggtc aacgcgggtt tcggcgacgc
     1021 gtggtcggcc gcgcagctcg tcgaatacgt gaacggctcc acgctgacca cgatggggcg
     1081 cctacgggcc gccaacgggc atcccgaacc ctacgacgtc cggttctggg gcatcgggaa
     1141 cgaggcatgg ggatcgtggc agatgggcgc gatgtcgctc gaccagttcg tcctgaagca
     1201 caacgagttc gccgaggcca tgcgggccgt cgacccctcg atcgtgctga tcgcgagcgg
     1261 ggcgatgccc gatgccatga cgggatcggg cgagtcgcag aagcgcacgg ggaagatcgt
     1321 gcccgagccc ctcgggcctg ccgactggac gggcggcctg ttcctgcaca gtctcgacca
     1381 catggactgg atcagtgagc actactactc gtacggcggc acgcgatacg acgttacgcg
     1441 gggcgagcag gtgcccgtcg atcccgacga gccgctggtc gactggatgc gtcgcccggc
     1501 caaccacgtc cggctcaagg tcgaggcgta cgaggactac gaggagcgca ttccggcgct
     1561 tcgcgacaag ccggttccca tcgtcatcga cgaatgggcg tttgccggag ggcccatccg
     1621 cccggattcg taccgcgccg tgccggcgta tgcgtgggcc ttccacgaga tgtttcgccg
     1681 gacggacctc ttcgtgatgg ccaacttcac gttcgccacc tcgatggtaa gcgcgacgcg
     1741 gacagaggcc gtcctgaatc cgacgggcct gctcttcaag ctctaccgcg accacttcgg
     1801 gacgatcccg gtgacggtga ccggcaactc gccccagtcc gcgccgaaat acccggtcgg
     1861 cggcgaagag cccaacctga acgcaggcag ccccaccttc ccgctggacg tggccgcggc
     1921 atggaccgaa gaccggagcg cacttacgat cgcggtcgtc aacccgacgg aatcggagct
     1981 ccccctggag ctcgggatct cgggcgcgga gctcgccggc acgggcaccc ggtggcgcat
     2041 ggccccggag agcctcgacg ccaccgtgtc cgtgggacgg aatccggagg tggtcgtcga
     2101 ggaggaaacg gtggcgggca cgctgtcgtc ggtcaccctc ccgcgcttca gcgtgacgat
     2161 ctaccgcctg gaggccaggt gagcggcatg caacgcggac cggcttgcgt cctggtcgcc
     2221 tggctcgtcc tcgcgagcct cgttccgtcc gccccggcgc tggcgcagga acgcgcccgc
     2281 aacccgatcc tctgggccga cgtcccggac ccgtcggtcg tccgcgtgga cagcgtctat
     2341 tacatgacga gcacgaccat gcacatgagc ccgggagtgc cggtcatgcg gtccacgaac
     2401 ctggtcgact ggaccacggt cggctacgtc tacgacgagc tcgcgaacgc cgacgcgctc
     2461 ttcatgcgtc agggccagga cgcctacggc cgcggcacct gggcgagcag cctccgccac
     2521 cgggacggcc tcttctacgt ggtcaccttc tcgtacacga cgggcgagac ctacgtcttc
     2581 acgaccgacg ccatcgagcg cggaccctgg cgcaaggcct cgctcggcgg gctctaccac
     2641 gacccttcgc ttctcttcga cgacgacggc cgcgtgtacc tcgtgtacgg agccgggaac
     2701 atccgcatcg tggagctggc cgcggatgcc cgcgccgtga agccgggcgg cgaggaccgc
     2761 gtgctgatcc cgaacgcggg cggcatcgcc ggatcggact tctgggtgcc cggcgagggc
     2821 tcgcacatcg tgaaggtcga cggccgctac tacctgttcc tgatcgtctg gccggccggc
     2881 ggcatgcgga cggagctcgt gtaccgggcc gactcgctcg aaggcccctg ggaagggcgg
     2941 attgtcctgg aggacgaggg catcgcgcag ggcggcgtcg tcgacacgcc ggacggccgc
     3001 tggtacgcca tgctcttcgg cgaccgcggt gcggtcggcc ggattccgta cctcgtcccc
     3061 gtcgcgtgga cggacggatg gccggtcttc ggaacggacg gagaggtgcc ggcgacgctc
     3121 gacatcccat cggacgggac cggcgtgcgg ggcatcgttg cgtcggacga gttcgacggg
     3181 acgcatctcg gccccgcgtg gcagtggaac caccagcccg acggcgatcg ctggtcgctc
     3241 ggcgcgaggc cggggtggct ccggatcgtg aacggaaggc ccggccgcag cctcgtggac
     3301 gcgcggaaca cgcccacgca gcgcaccttc gggccccgga gttccgcgac caccctcctc
     3361 gacgcgggcg gcctccagga cggcgacgcg gccgggctcg ccattctcat ggagcactac
     3421 gcgtggatcg gcgtcgtcag ggagggcggc cgagcgagcc tggtcgtgac cgcgctgcag
     3481 gacggcgggg agacggagac cgcccgcgtg ccgcttgacc gggaggtcgt gtacctgcgg
     3541 gtggacgccg atttccgcga cttgaaggac gaggccacct tctcgtacag cctggacggc
     3601 aaggcgtgga ttcccgtcgg cgagcccttc gagatgcgct aca
//
LOCUS       DBNHQZ010000050         3331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_13860, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000050 DBNHQZ010000000
VERSION     DBNHQZ010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_13860"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1624
                     /locus_tag="AC16YU_01220"
     CDS             89..1624
                     /locus_tag="AC16YU_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR6063870.1"
                     /translation="MPGTQPKPTPESVLRDVFGFPDFRPGQREVIEAVLAGRDCIALM
                     PTGAGKSLTYQVPARILDGTVLVLSPLISLMKDQVDAVSALGLRATAINSTLPAEERR
                     ARMEGFRAGAWELVYLAPEALDGHFGDFVRGCPVSLLVVDEAHCISQWGHDFRPSYRR
                     LAGLKEQLDVPVLALTATATRRVAVDILRQLGMKKPAGYRGSFFRPNLRILARKKGQG
                     GNTRREILGLVRAHAGESGIVYCQSRRSVEQIAAFLAAQGIRALPYHAGLDAEDRIRN
                     QEAFQRDDVEVIVATIAFGMGIDKSNVRFVIHRDMPRDVESWYQEIGRAGRDGLPSDC
                     VLFYSWADVKLQERFLDGLEGEPLRQRQMEATRRLFRLADGPACRHRALVAHFGEEIA
                     DCGTSCDVCTGKTIPELVKDVTLATGTTGRRRRSGTGPRPSADAGDVDTALFERLRAL
                     RKTLADEAGVPAYVIFGDRTLVEMAARRPTTRDQLLDVSGVGLVKLERYGDAFLEVIA
                     GRA"
     gene            complement(1693..2205)
                     /locus_tag="AC16YU_01225"
     CDS             complement(1693..2205)
                     /locus_tag="AC16YU_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR6063871.1"
                     /translation="MSTPKSHPDPEVRSLYGRAVRRFDRLDRSIVRWMASYGIPILRV
                     GLGVIFVWFGLLKFFPGMSPAEGLVRNTVYFVDPDLFLPVLAAWETLIGVGLIAGRFM
                     RTTLLLLFLQMPGTALPLVVLPEVVWTAFPFALTLEGQYIIKNLVLIGAGIVLGATVR
                     GGRLSADPAD"
     gene            2549..>3331
                     /locus_tag="AC16YU_01230"
     CDS             2549..>3331
                     /locus_tag="AC16YU_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6063872.1"
                     /translation="MQVETRQTEQALIISLAGEWDIESTTPRFDRLVEDASPGREVRT
                     VAFDTMDLGKWDSSLLTFLLQGIDYCETHELVLEKESLPENISLLLDLARALPEQAVV
                     HEAAPAAPLARLGNRVVSAMDGSLGSVAFIGEFVGSLVRLLSFRVRLRWRDFWVSVQS
                     NSFGALPIVSLIAFLVGVIISFLGAVVLRKFGAGYYVSYLVGYGILREMGALMTGIIM
                     AGRTGAGFAAELGSMKITEEVDALRTFGISPIDHLVLPRVLGL"
BASE COUNT          513 a         1126 c         1161 g          531 t
ORIGIN      
        1 cgtgtgaggg acggttgccc agacccagtg ggcgaacgcg gcccggagcc gcctgttgaa
       61 tgacagcccc gcgaaacgcg ccgcccccat gcccggaacc cagccgaagc ccacgcccga
      121 atccgtcctt cgcgacgtgt tcggcttccc ggatttccgt ccggggcagc gcgaggtcat
      181 cgaggccgtg ctcgccggtc gcgactgcat cgcgctcatg cccacgggcg ccgggaagtc
      241 cctgacgtac caggtcccgg cgcgcatcct ggacggcacg gtgctggtcc tctcgccgct
      301 catctcgctg atgaaggacc aggtggacgc cgtctcggcg ctgggcctgc gggcgacggc
      361 catcaactcg acccttccgg ccgaggagcg cagggcccgg atggagggat tccgggccgg
      421 ggcctgggag ctcgtgtacc tcgctccgga ggcgctggac ggccatttcg gcgatttcgt
      481 ccggggttgc cccgtctcgc tactggtcgt ggacgaggcc cactgcatca gccagtgggg
      541 gcacgacttc cggccgtcgt accggcgcct ggccggcctc aaggagcagt tggacgtccc
      601 ggtgctcgcg ctcaccgcca cggccacccg gcgggtcgcc gtggacatcc tgcgccagct
      661 cggcatgaag aagccggcgg gataccgggg ctcgttcttc cgcccaaacc tgcgcatact
      721 cgcccgcaag aagggccagg gcgggaatac gcgcagggag atcctgggcc tggtccgcgc
      781 gcatgcgggt gagagcggca tcgtgtactg ccagagtcga cggagcgtcg agcagatcgc
      841 ggccttcctg gccgcgcaag gcatccgggc cctgccctac cacgccgggc tcgacgcgga
      901 ggatcgcatc cggaaccagg aggcctttca gcgggacgac gtcgaggtga tcgtcgccac
      961 catcgcgttc ggcatgggga tcgacaagtc caacgtccgc ttcgtgatcc accgggacat
     1021 gcccagggac gtggaatcct ggtaccagga gattggccgc gccgggcgcg acggcctgcc
     1081 cagcgactgc gtgctgttct attcgtgggc cgacgtcaag ctgcaggagc gcttcctgga
     1141 tgggctcgag ggcgaaccgc tccggcagcg gcagatggag gctacgcgcc gcctgttccg
     1201 gctcgcggac ggcccggcgt gtcgccaccg cgccctcgtc gcccatttcg gagaggagat
     1261 cgcggactgc gggacctcct gcgacgtgtg cacggggaag acgatcccgg agctcgtgaa
     1321 ggatgtcacg ctcgccacgg gcacgaccgg tcgcaggcgc cgatcgggca ccggcccgcg
     1381 gccgtccgcc gacgccggcg acgtggatac cgccctgttc gagcggctgc gcgccctccg
     1441 gaagacgctc gccgacgagg cgggagttcc cgcctacgtc atcttcggcg accggacgct
     1501 cgtcgagatg gcggcgcgcc ggcccacgac gcgcgaccag ctcctcgacg tgtccggcgt
     1561 cggcctggtc aagctggagc ggtatggcga cgcctttctc gaggtgatcg ccggccgcgc
     1621 ctagggcccg cgccgccggc gggcgcacgg ccggggaccg ctccggggcg cctgcggcgg
     1681 tcgcgcgtcc ggtcagtccg ccggatcggc cgagagccgg cctccccgga ccgtggcgcc
     1741 gagcacgatt ccggccccga tgagcaccag gttcttgatg atgtactggc cctccagcgt
     1801 gagcgcgaag gggaaggcgg tccagacgac ctcgggcagc accacgagcg gcagggccgt
     1861 gcccggcatc tgcaggaaga gcagcaggag cgtcgtgcgc atgaaccggc cggcgatgag
     1921 ccccacaccg atcagggtct cccacgcggc caggaccggc aggaagaggt ccggatccac
     1981 gaagtagacc gtgttccgga ccaggccctc cgcggggctc atccccggaa agaacttgag
     2041 cagcccgaac cacacgaata tgacgccgag gcccacccgc aggatgggga ttccgtacga
     2101 cgccatccag cggacgatgg atcggtccag gcggtcaaag cgccgaacgg cgcggccgta
     2161 caggctgcgg acttccggat ccggatgact cttcggggta ctcatggcgg gatgggggtg
     2221 tgattccgct gtccgtgcac gtcgtgagta cgtgccgcct ccccgtccgg tgtccgtgcc
     2281 cgtatgaggc ctcgtcgaat acgggcggcg gcgacagcgg ccctcgtggg tgtcgcgatc
     2341 atgccttagc tctgaatcgt ccgtgcccgg ttcgtacgcg cccgcgtcga actagaagca
     2401 gaatgcctac atgcggcgag gacaactgac ctcacgccga ccgccggacc gaacgtaccc
     2461 tgtccaccaa atggaggcag tccgcctctg tccgtgtcat ggtgcgccat ctacgcgtgc
     2521 gcgcacgaga ggggtgccgt tcgttcccat gcaggtagag actcgtcaaa cggaacaggc
     2581 tttgatcatc tcgctggccg gtgagtggga catcgagagc acgacgccac gcttcgaccg
     2641 gctcgtcgag gacgcatccc ccgggcgcga ggtccggaca gtggccttcg acacgatgga
     2701 cctgggaaag tgggacagca gtctgctcac attcctgctg cagggcatcg attactgcga
     2761 gacccacgaa ctcgtgctgg agaaggagag cctgccggag aacatttcgc tcctcctcga
     2821 cctcgcacgg gcccttccgg agcaggcggt cgttcacgaa gcggcacctg ccgcgcctct
     2881 cgcccggctc gggaatcgcg tggtttcggc catggacggg tccctcgggt cagtcgcctt
     2941 catcggggag ttcgtgggca gcctggtccg attgctcagt ttccgggtcc ggctgcgctg
     3001 gcgggatttc tgggtttccg ttcagtcgaa cagctttggt gccctcccga tcgtctcgct
     3061 gatcgcgttc ctcgtcgggg tgatcatctc gtttctcggg gcggtcgtgc tgcgcaagtt
     3121 cggggccggc tactacgtgt cgtacctggt cgggtacggc atactgcggg agatgggagc
     3181 cctgatgacg ggaatcatca tggccggccg gacgggtgcc ggcttcgcgg ccgagctggg
     3241 cagcatgaag atcaccgaag aggttgacgc cctgagaacc ttcggcatct cgccgatcga
     3301 ccacctcgtg ctgcccaggg tcctggggct c
//
LOCUS       DBNHQZ010000051         5369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1910528, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000051 DBNHQZ010000000
VERSION     DBNHQZ010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1910528"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC16YU_01235"
     CDS             <1..690
                     /locus_tag="AC16YU_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6063873.1"
                     /translation="SPLEAALQDEPDRVERLNALAQAYQATGRPAVNVERLYRQALRI
                     QPARGDVRLNYGQFLETRGRTEEALAEYRQAAAEGPWRALAEFNLGSAHARSGNQEEA
                     TAALRRALHLDPTLVEAWGTLGALYAQARMADSAGMAFERAVRIAPRDALALANLGAF
                     YVNLEQSAEGIPLLRDAVTIRPDYVDALANLALAWFRVDSLREARAAAERALAVDPGN
                     ALARQVILATE"
     gene            complement(671..1678)
                     /locus_tag="AC16YU_01240"
     CDS             complement(671..1678)
                     /locus_tag="AC16YU_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HIR6063874.1"
                     /translation="MESPLDVVVVGAGPVGLACANECGRAGLRARVVEKGAVVNSLVG
                     YPTGMEFFSTPDLLEIGGHPFPTSRYKPLREEALDYYRKVAWAERLGLALYERVLRLD
                     GSEGAFEVVTDRGSHRAGRVIVATGFFDVPNPLGVPGEDLPKVSHYFREPYPFTGQDV
                     AVVGAKNSAAKAALQCFRHGARVTMIVRGPSLSESIKYWIRPDLENRIAEGAITALFG
                     TEVTAIERDSIALRTPGGPGTIPNDWVLALTGYRPDYRLLDALGIGVGDDESRTPAFD
                     PGTFETDRPGVFLAGTICGGLATSRWFIENGRHHARVIAEEVAGRRERSGPSHPTPSP
                     G"
     gene            complement(1678..2184)
                     /gene="tadA"
                     /locus_tag="AC16YU_01245"
     CDS             complement(1678..2184)
                     /gene="tadA"
                     /locus_tag="AC16YU_01245"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR6063875.1"
                     /translation="MTGGLDALAAPHRRWMKAALREAEIALEEGDVPVGAVIVRGDQV
                     VGRGHNMVERLQDPTAHAELLAITAAASTIGSKFLEGCTLYVTLEPCPMCAGAIVWSR
                     LERLVFGAFDEKAGAASTLYNIPQDARLNHRVDIISGVEAEPAEAMLREFFRKLREES
                     APNGNRKS"
     gene            complement(2181..3434)
                     /locus_tag="AC16YU_01250"
     CDS             complement(2181..3434)
                     /locus_tag="AC16YU_01250"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIR6063876.1"
                     /translation="MAPTHLPRRLNVTSETGHLEQVIVHTPGAEMERIAPGSLQHVLF
                     EDILWVEHARKEHELMCRIFETVIAEPGGVLQMGDLVTESFASDEARQDFVRNLCLAA
                     PERNLQVFEKELSALSPASLSTFALTGVSDLPIVAQPLPNLMFTRDVAAVVGDHLVLS
                     HPATAARARESAVMNAVVMHHPGFAAYRDHVIRLPKGVTFEGGDLIVVNDRTVLIGHS
                     QRTSFGGVTSIVRALFERTGIRNVIMVNLPKERSCMHLDTVFTFVSPDECVVFPPLIG
                     LRGLGNVVRYAPGDGPDDLRCHVHADLAEALATVLETPVRFYPCGGDDPLSQQREQWT
                     DGANLFAIAPGVVIGYERNARTFEELRANGFRVVTAEGFLAYHAQSAFASGERIAIKL
                     DGHELSRGRGGPRCMTLPLSRGPLS"
     gene            complement(3511..3705)
                     /locus_tag="AC16YU_01255"
     CDS             complement(3511..3705)
                     /locus_tag="AC16YU_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063877.1"
                     /translation="MRSLDIQQILRWIVTAIVLMVAIVLLGVVLKMAGFLLSYAIKGL
                     LILLLIAIVVRLIGALQQRR"
     gene            complement(3719..5032)
                     /locus_tag="AC16YU_01260"
     CDS             complement(3719..5032)
                     /locus_tag="AC16YU_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR6063878.1"
                     /translation="MARIPVLAVALVAVACERPFVDVRPPDIEILEPDPASVIPDAEI
                     LLRVKATSFRGVSRVLVDGQDAEAEGPDTWSLPLTLTRGLNTFVLEAIDPETVSTVDT
                     LRLLRLSLGRQPGPTWSAAVGGHTATRLGDGRLLLAGGAAAPDGPATGATWLMDPASG
                     HISPGPPLVHARTGHTATLLPDGRLVVIGGAGTDGPDSVGDLVEAVEVLEPDEESFRE
                     LPVHGDPVRRAFHTAELRLTESGPVVDVIGGTGDVRYGQEPRLDVRDDLRSFRVHADS
                     VVALVPWYGVFLDAMTGHTQTALHVAGPGSPNRFLLSGTVFGATVQYPVNYVMDTRSS
                     PTILLEPVSPPVPVRNEHVAVRLADGRVLLLGGRTPDSDVDGAGALFDLSLGRYLRVR
                     AGVAGSPEPRRRHTATLLPDGRILLMGGYDATGAAETTSDRLYIE"
     gene            complement(5047..>5369)
                     /locus_tag="AC16YU_01265"
     CDS             complement(5047..>5369)
                     /locus_tag="AC16YU_01265"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0106029 - tRNA pseudouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR6063879.1"
                     /translation="LVGTHDFDAFCRSASDTENRICTVTRAAWSADRRPGDWRFDIEA
                     DRFLHGMVRAVVGTLLEIGRERRPEDDVSRILESRDRRLAGPAASPRGLVLETVTYDG
                     EVTA"
BASE COUNT          818 a         1893 c         1891 g          767 t
ORIGIN      
        1 tcgccgctcg aggcagcgct ccaggacgag cccgatcgcg tggagcgcct gaacgcgctg
       61 gcccaggcct accaggccac ggggcggcct gcggtgaacg tcgagcggct gtaccgccag
      121 gcgctccgca tccagccggc caggggagac gtccggctga attacggcca gttcctggag
      181 acccggggca ggactgagga ggcgctcgcg gaataccgcc aggccgccgc ggaggggccc
      241 tggcgggcgc tggccgagtt caacctggga tcggcccacg cccggtccgg caaccaggaa
      301 gaggctacag cggcgctccg gcgcgccctg cacctggacc ccacgctggt cgaggcctgg
      361 ggcaccctgg gggccctgta tgcgcaggcg aggatggccg actcggcagg catggcgttc
      421 gagcgggcgg tccgcatcgc gccccgggat gccctggccc tggccaacct cggagcgttc
      481 tacgtcaatc tcgagcagtc cgctgagggc atcccccttc tgcgcgacgc cgtgaccatt
      541 cggcccgatt acgtggacgc gctggccaac ctggccctgg cctggttccg ggtggacagc
      601 cttcgcgaag cccgcgcggc agcggagcgc gccctggccg tcgacccggg gaacgccctg
      661 gcgaggcagg tcatcctggc gacggagtag ggtgggacgg gccgctccgc tcccgccttc
      721 cagcgacctc ctccgcgatc acccgggcgt ggtgccgccc gttctcgatg aaccaccggc
      781 tcgtcgcgag gcctccgcag atcgtcccgg cgaggaaaac gcccggccga tccgtctcga
      841 aagtgcccgg gtcgaaggcc ggcgtacggg actcgtcgtc gcccacgccg atgcccaggg
      901 catcgaggag ccggtagtcg ggacggtagc cggtcagggc gaggacccag tcattcggaa
      961 tcgttcccgg tccgcccggt gtccggagcg caatcgagtc ccgttcgatg gcggtcacct
     1021 cggtgccgaa gagagccgtg atcgcgcctt ccgcgatccg gttctccagg tccggccgga
     1081 tccagtactt gatgctctcc gacagcgacg ggccgcgtac gatcatcgtg acgcgggccc
     1141 cgtgacggaa gcactgcagc gcagccttgg ctgccgagtt cttcgcgccg acgacggcca
     1201 cgtcctgccc ggtgaagggg tacggctccc ggaagtaatg ggagaccttg ggcaggtcct
     1261 ccccggggac tccgagggga ttggggacgt cgaagaagcc cgtcgcgacg atcacccggc
     1321 ccgcgcggtg cgacccccgg tcggtcacga cctcgaaggc cccctccgag ccgtccaggc
     1381 gcagtacccg ctcgtagagc gccagaccga gccgctcggc ccaggccacc ttccggtagt
     1441 agtcgagcgc ctcttcgcgc agaggcttgt agcgcgaggt cggaaacggg tgcccgccga
     1501 tctccaggag gtcgggggtg gagaagaact ccatgccggt cggatagccc acgagcgagt
     1561 tgacgaccgc ccccttctcg acgacccggg cgcgcagccc ggcccgcccg cactcgttgg
     1621 cacacgcgag ccccacgggc ccggcgccca cgacgacgac gtccagcggg gattccatca
     1681 gctcttccgg tttccgttcg gcgcagattc ctcgcggagc ttccggaaga actcgcggag
     1741 catcgcctcc gcgggctcgg cctccacccc cgagatgatg tccacccggt ggttcagcct
     1801 ggcgtcctgc ggaatgttgt agagcgtgga cgccgcgccc gccttctcgt cgaaagcccc
     1861 gaagaccagg cgttcgagcc gtgaccatac gatcgcgccg gcgcacatgg gacagggctc
     1921 gagcgtcacg tacagcgtgc agccctcgag gaacttcgag ccgatggtcg acgcggccgc
     1981 cgtgatcgca aggagctcgg cgtgcgcggt cgggtcctgg aggcgctcca ccatgttgtg
     2041 tccgcggccc acgacctggt cgccgcgtac gatcacggcg ccgacgggca cgtccccctc
     2101 ctcgagggcg atctccgctt cccgcagggc cgccttcatc cagcggcggt gcggcgcggc
     2161 gagcgcatcg aggccccccg tcatgacagg ggcccccgcg acaggggaag ggtcatgcag
     2221 cgcggcccgc cgcggccgcg cgagagctcg tgaccgtcca gcttgatcgc gatccgctcg
     2281 ccggacgcga aggcggactg cgcatggtag gcgaggaagc cctcggccgt cacgacccgg
     2341 aagccgtttg cccggagctc ctcgaaggtc ctcgcgttgc gctcgtatcc gatgaccacg
     2401 cccggagcga tcgcgaacag gtttgcgccg tcggtccact gctcccgctg ctggctgagc
     2461 gggtcgtcac cgccgcacgg atagaagcgg accggtgtct cgagcacggt cgcgagggcc
     2521 tcggccaggt cggcgtgcac gtggcaccgg agatcgtcgg gaccgtctcc cggagcatag
     2581 cggacgacgt ttcccaggcc ccgaagcccg atgagcggcg ggaatacgac gcactcgtcc
     2641 ggggagacga acgtgaacac ggtgtccagg tgcatgcagg agcgttcctt gggaaggttc
     2701 accatgatca cgttccggat cccggtgcgt tcgaagagcg cgcggacgat ggacgtgacg
     2761 cccccgaacg acgtgcgctg ggaatgtccg ataagcacgg tccggtcatt caccacgatc
     2821 aggtcgcctc cctcgaacgt cactcccttc ggcaggcgga tcacgtggtc gcgataggcg
     2881 gcgaagcccg ggtggtgcat gaccacggcg ttcatgacgg cgctctcccg tgcccgggcg
     2941 gccgtcgcgg ggtggctgag cacgaggtga tcgccgacga cggccgccac gtcccgcgtg
     3001 aacatgaggt tcgggagcgg ctgtgccacg atgggaagat ccgacacgcc ggtgagcgcg
     3061 aacgtcgaga gcgacgcggg cgagagggcc gacagctcct tctcgaagac ctggaggttg
     3121 cgctcgggcg cggcgaggca caggttgcgg acgaagtcct ggcgggcctc gtccgacgcg
     3181 aacgattccg tgaccaggtc gcccatctgg agcacgccgc cgggctcggc gatcaccgtc
     3241 tcgaagatcc ggcacatcag ctcgtgctcc ttcctcgcgt gctccaccca gaggatgtcc
     3301 tcgaaaagga cgtgctgcag gctgccaggg gcgatccgct ccatttcggc gcccggggtg
     3361 tgcacgatca cctgctccag gtgacccgtt tcggacgtga cgttcagccg gcggggcaag
     3421 tgcgtcggag ccatacccat ggggactgga atgccggcac gcgggtcgtg cctccgggcg
     3481 ccgtacggca gccctcgccg gatgatcccc ctagcggcgc tgctgcagtg cgccgatgag
     3541 ccgcacgacg atcgcgatca ggaggaggat gagaaggccc ttgatggcgt acgaaagcag
     3601 gaaaccggcc atcttgagca cgacgcccag cagcacgatc gccaccatca ggacgatcgc
     3661 ggtaacgatc cagcgcagga tctgctgaat gtcgaggctt ctcatcggcg cttccgtttc
     3721 actcaatata caaacggtcg gacgtggtct ctgccgcccc ggtggcatcg tatccgccca
     3781 tcagcagtat tcgtccgtcg ggcagcaaag ttgcagtgtg gcggcgcctg ggttccgggc
     3841 ttcccgccac cccggcgcgg acgcggaggt accgtcccag ggacaggtcg aagagggcgc
     3901 cggcgccgtc cacgtcgctg tccggggtgc gtccgcccag gagcagcacc cgcccgtcgg
     3961 ccaggcggac cgccacatgc tcgttccgca ccggtacggg tggcgagacc ggttcgagca
     4021 ggatggtcgg ggaactgcgc gtgtccatga cgtagttgac ggggtactgc accgtcgcgc
     4081 cgaacaccgt cccggatagc aggaaccggt tggggcttcc ggggcccgcc acgtggagcg
     4141 cggtctgggt gtggcccgtc atcgcgtcca ggaacactcc gtaccagggc acgagcgcga
     4201 ccaccgagtc ggcatgcacg cggaagctcc tgaggtcgtc ccggacgtcg agccggggct
     4261 cctggccgta ccggacgtcc cccgtgccgc cgatgacgtc gacgaccggt ccggactccg
     4321 tcaagcgcag ctccgccgtg tggaacgccc gccgcaccgg gtcaccgtgc acgggaagct
     4381 cacggaacga ctcttcgtcg ggctccagga cctcgacggc ctcgaccagg tcgcccacgg
     4441 agtccggccc gtccgtcccg gcgcctccga tgaccaccag gcggccgtcg ggcaggagcg
     4501 tagccgtgtg gcccgtccgc gcgtggacca gcggcggtcc gggggagatg tggcccgagg
     4561 ccggatccat cagccaggtc gcgccggtag ccgggccgtc gggcgcggcc gctccgcccg
     4621 ccagaaggag ccggccgtcc cccaggcgcg tcgccgtgtg tccgcccacc gccgcgctcc
     4681 acgtggggcc gggctggcgc ccgagggaca gccgcaggag ccggagcgtg tccacggtcg
     4741 agaccgtctc cgggtcgatc gcctcgagaa cgaacgtatt caggccacgg gtcagcgtga
     4801 ggggaaggct ccaggtgtcc gggccctcgg cctcggcgtc ctgtccgtcg acgagcacgc
     4861 ggctcactcc ccggaacgat gtcgccttga cgcgcagcag gatctccgcg tccgggatga
     4921 cgctcgcggg atccggctcc aggatctcga tatccggcgg acggacgtcc acgaacggcc
     4981 gctcgcaggc cacggcgacg agcgcgaccg cgaggacggg tatccgcgcc atggtaccgg
     5041 ggcggctcac gcggtgacct cgccgtcgta cgtcacggtc tcgagcacga gcccgcgcgg
     5101 actcgcggcc ggtcccgcca gccgccggtc ccggctctcc aggatgcggc tcacgtcgtc
     5161 ttcgggtcgg cgctcccggc cgatctcgag cagcgttccg acgacggccc gcaccatgcc
     5221 gtgcaggaag cggtcggcct cgatgtcgaa gcgccagtcg ccgggccgcc ggtccgcgga
     5281 ccaggcggcc cgcgtcaccg tgcagatccg gttctcggtg tcgctcgcgg atcggcagaa
     5341 ggcatcgaag tcgtgcgtcc cgaccaggc
//
LOCUS       DBNHQZ010000052         2999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1910540, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000052 DBNHQZ010000000
VERSION     DBNHQZ010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1910540"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="AC16YU_01270"
     CDS             complement(<1..210)
                     /locus_tag="AC16YU_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR6063880.1"
                     /translation="MEPYVLHKSANSGWIEVICGSMFSGKTEELIRRMRRARIARQRV
                     EIFKPAIDQRFSDFEVVSHDESAIPS"
     gene            complement(277..1056)
                     /locus_tag="AC16YU_01275"
     CDS             complement(277..1056)
                     /locus_tag="AC16YU_01275"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6063881.1"
                     /translation="MRRSAPDLLRAALLVVLLVAAAAGRSGAQPAEAVRLYEEGTQRL
                     LAGNYSGAVGSYRSAMDLGVGSAALYYNVGVAYYRLDRLGEAIRFLERARRLAPSDRR
                     ILHNLEIARSRIPERFARLPVRFWTRGWQAIVSVLGVGGLFAIGLVLWWLPFGLVAWR
                     VRRGRRTPWTGRIGWPSAALAVLFLAAATAASVRSPFGDQAVIIEPTAPLYTSAAGDD
                     AGIVLHEGLTVDIEARDGMWLRVRLPNGVTGWVQAEAAAEV"
     gene            complement(1056..2915)
                     /locus_tag="AC16YU_01280"
     CDS             complement(1056..2915)
                     /locus_tag="AC16YU_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6063882.1"
                     /translation="MRRFHIILALMAGLAGSAAAQEPQVLASVSGTTIGMEETVTFTI
                     EIRAETLSGVVTPSPPQAGGLALVQAFPSTQQSMSVVNGRVSQGVAYSWTYRPIREGR
                     TSFPAVSVRVGDREYHTTPVDVEVVPQAQRPQRRAAPGRVDPFASFRTPPDDEPAEAL
                     VPDENDLFIRVVPSSRSPFQNEQVLIQYELFFRDGIQLRSSRLTDSWDAEGFWREELD
                     VDARPIPRVVVENGLRYNTITLKRAAVFPTRPGELQVDPLKIETEAQLPSRSRDPFAQ
                     FFSLRSQYRLVELSSPALTIEARALPPGAPASFRGAVGQFGMIVDLDRTEVPVGGSVQ
                     MTVTLTGAGNIATLAPPEVHVPGAFERYDPQVETVIDRSGDRLQGSKRFRYVFVARSN
                     GEYEFPGVSFSYLDPASGAYREVHSDPGTVRVTGSADDSPIARATSGGFPVDDIAGPW
                     LEPVTWMRTDRTPLHRQAWPYAALLLPALLAGALVVADRRARRLAANPALGRHRRAHP
                     LARKHLRLAVELQEARKYRAFYEEVSGAILGFIGNRLDVAEHGLTRERLDDRLARSGV
                     SPEIRRELRAFLDACDRARFGPLDPEPGAVEVDRDRASRLLVLLDEALEAGRS"
BASE COUNT          470 a         1064 c         1073 g          392 t
ORIGIN      
        1 ggatggaatc gccgactcgt cgtgcgatac cacctcgaag tcggagaacc gctggtcgat
       61 ggcgggcttg aagatctcga cccgctgccg cgcgatgcgg gcacgccgca tccggcggat
      121 cagctcctcc gtcttgccgg agaacatcga gccgcagatg acctcgatcc agccggagtt
      181 cgccgatttg tggaggacgt agggttccat ggatgccgac cgggtccggg gtcgaagata
      241 cgcggcagcc ggcattcccg gcccccggtg gatgccttac acctccgcag cggcctccgc
      301 ctgcacccag ccggtcacgc cgttcggcag gcgtacccgg agccacatgc cgtcccgggc
      361 ctcgatatcg acggtcagcc cctcgtgcag cacgatgccg gcgtcgtccc cggccgcgga
      421 cgtgtaaagc ggcgcggtgg gttcgatgat caccgcctgg tcgccgaagg gcgagcggac
      481 ggatgcggcg gtcgcggcgg cgaggaagag caccgccagc gctgcggacg gccacccgat
      541 gcgtcccgtc cagggcgtcc gccggccccg gcgtacccgc catgccacga gcccgaacgg
      601 cagccaccag agcacgagcc cgatcgcgaa caggcccccg acgccgagga cgctcacgat
      661 cgcctgccag ccccgggtcc agaatcggac gggaagccgc gcgaagcgct ccgggatgcg
      721 cgatcgggcg atctcgaggt tgtggaggat ccggcgatcc gacggcgcga ggcggcgcgc
      781 ccgctcgagg aaccggatcg cttcgccgag ccggtccagc cggtagtacg cgacgccaac
      841 gttgtagtag agggccgccg atccgacgcc caggtccatc gccgagcggt acgagccgac
      901 cgcgcccgag taattgcccg cgagcagccg ctgggtgccc tcctcgtaca gccggacggc
      961 ctcggccggc tgggcccctg agcgcccggc ggcggccgcg accaggagga ccacaaggag
     1021 ggccgcacgg aggaggtctg gagcgcttcg ccgcatcagg atctcccggc ctcgagggcc
     1081 tcgtcgagca gcaccaggag gcgggacgcc cggtcccggt cgacctccac cgcgcccggc
     1141 tccggatcga gcggcccgaa ccgggcccgg tcgcaggcgt cgaggaacgc gcgcagctca
     1201 cgccggatct ccggggacac gcccgagcgt gcgaggcggt cgtccagccg ctcccgcgtc
     1261 aggccgtgct ccgcgacgtc caggcggttg ccgatgaagc cgagaatggc cccggagacc
     1321 tcctcgtaga aggcgcggta cttccgtgcc tcctggagct cgaccgcgag gcgcaggtgc
     1381 ttgcgcgcca ggggatgggc ccttcgatgc ctgcccaggg ccggattcgc ggccagccga
     1441 cgcgcccggc ggtccgccac gaccagggcg cccgcgagga gcgcgggcag caggagcgcc
     1501 gcgtacggcc aggcctgccg gtggagggga gtacggtccg tgcgcatcca cgtgaccggc
     1561 tccagccagg gacccgcgat gtcgtcgacg gggaagcccc cggacgtggc gcgcgcgatc
     1621 ggcgagtcgt ccgcgctgcc cgtgacgcga acggttcccg gatcggagtg aacctcgcgg
     1681 taggcccccg acgcgggatc caggtaggag aacgacacgc cggggaactc gtactcaccg
     1741 ttcgatcggg ccacgaacac gtagcgaaac cgcttggaac cctgcaggcg atcgccggac
     1801 cggtcgataa cggtctcgac ctggggatcg taccgttcga aggcacccgg cacgtgcacc
     1861 tcgggagggg ccagggtcgc gatgttgccg gcgcccgtca gcgtgaccgt catctggacg
     1921 gagccgccaa ccggcacctc cgtccggtcc aggtcgacga tcatgccgaa ctgaccgact
     1981 gccccccgga aggaggcggg ggcgcccggc gggagcgccc tcgcctcgat cgtgagcgcg
     2041 ggcgacgaaa gctcgaccag gcggtactgg ctccggagcg agaagaactg ggcgaaggga
     2101 tcgcggctgc ggcttggaag ctgcgcctcg gtttcgatct tcaggggatc tacctggagc
     2161 tctcccgggc gcgtcggaaa aacggccgcc cgcttcagcg tgatcgtgtt gtagcgcagc
     2221 ccgttctcca cgacgaccct cggaatgggt cgagcgtcca cgtcgagctc ctcgcgccag
     2281 aagccctccg cgtcccagga gtccgtaagc cggctggacc gcagctggat gccgtcgcgg
     2341 aagaagagct cgtactggat cagcacctgc tcgttctgga acggcgagcg ggacgagggc
     2401 acgacgcgga tgaacaggtc gttctcgtcg ggcaccaggg cctcggccgg ctcgtcgtcc
     2461 ggcggcgtcc tgaagctggc gaacggatcg acccggccgg gcgcggcgcg acgctgcggg
     2521 cgctgcgcct gcgggacgac ctcgacgtcg acgggggtcg tgtgatactc ccggtcgccc
     2581 acgcgtacgg acacggccgg aaagctggtc ctgccctccc ggatgggccg gtacgtccac
     2641 gaatacgcca cgccctggct gacgcgtccg ttcacgacgg acatggactg ctgcgtgctc
     2701 ggaaacgcct ggaccagggc gagccccccg gcctggggcg gcgacggagt cacgacgccg
     2761 ctcagcgtct cggcccggat ctcgatcgtg aacgtcacgg tctcctccat cccgatggtc
     2821 gtgccgctca ccgacgcgag gacctgcggc tcctgggccg cggcactgcc ggccagcccg
     2881 gccatcaggg cgaggatgat atggaaccga cgcatccgca tggacatccg gcgagaggcg
     2941 cgcctaccag tccttctcga cccggcgggg ccgcgccttc atcttctgca cctggcgga
//
LOCUS       DBNHQZ010000053         2545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1097682, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000053 DBNHQZ010000000
VERSION     DBNHQZ010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1097682"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..737
                     /locus_tag="AC16YU_01285"
     CDS             306..737
                     /locus_tag="AC16YU_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR6063883.1"
                     /translation="MVIFLLTAGSAAAQVDVRSDLPAVGGTEAPSIRLENMGLSDGIA
                     QGTIYDIIQDRHGYIWFGTQGGLHRYDGQTFEVFKPEPFDTTSISDGWVIGVDEGRDG
                     SLWLATHGGGLDRGGRARRWRVVRGPDSGLIAAFSVLVKSL"
     gene            788..>2545
                     /locus_tag="AC16YU_01290"
     CDS             788..>2545
                     /locus_tag="AC16YU_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6063884.1"
                     /translation="MIGLHLAQYRIDAELGRGGMGIVYRATDAKLGRTVALKVLPASA
                     LASEDDRARFFREAQAAAQLSHPNIAGVHQIDEAVPVDEHGMKVGASDGPRPFIAMEF
                     IDGETVAERAKRGPFQLREAVTVAGQVAEALKAAHGKGIVHRDIKSANVMLTGDGVAK
                     VLDFGLAKTNQSTMLTRMGSTLGTVAYMSPEQARGQEVDGRSDLYSLGTMLYEMVAGR
                     LPFAGDYEQAVLYGILNEPPEPLTAVRTGVPMELERIVAKLLSKEAEYRYQTAADLIA
                     DLKALDVSGSGVSRRSMPSIPAAEGAASAKSSFPVWAWAIAATCLVAGFAAAWLIASR
                     GGQGLAQYPTVHAEIALGGGRMPQAMSISPDGTLLAYSDASDGAVYVRDLETGEERMV
                     PNTRGIIELDFSPDGQWLLLDEQLSILRASVSGGSPVRLHVVASNAPHSSWVDSERIL
                     FDDASSIWMMSISDGSVTPVVTADSSEGELRLLWGRVSPDGSRLFARIIRDPDTPKFG
                     IYSFPDGEVIAKDDWDVVSPRYLETGHLILLENSDLMVRPLDIASGRFLGAPVRIAEA
                     ISETNWDLSSWGQLVYGPIT"
BASE COUNT          406 a          821 c          881 g          437 t
ORIGIN      
        1 tcgcgagcat tggcggcaac ttccattact ctctccatcc cgacggtgag cgcatactca
       61 tccagggtcc gatgctctcc caggcaagcc tcaaatcgac catccaggtc tggttcaact
      121 ggttcacgac ggcggatcga ctggcgccac cgagccggtg agcggctccc cggcccggcg
      181 ccgtttcttg aagccctcgt gaagcgccgg gacgcgccgt ctctttggca ccctaccccc
      241 gatctggaac tcgcattccc ggtcccgaga cgtcgtcggc tcccgtaggc cgatcgaagc
      301 ttcccttggt catcttcctg ctcacggccg gttctgccgc cgcgcaggtg gacgttcggt
      361 ccgatctccc ggcggtcggg ggcaccgagg cgccgtccat ccgcctcgag aacatgggcc
      421 tgagcgacgg catcgcccag gggaccatat acgacatcat ccaggatcgc cacgggtaca
      481 tctggttcgg tacccagggt gggctgcacc gctacgacgg gcagacgttc gaggtgttca
      541 agccggagcc gttcgacacg acctcgattt cggatggttg ggtgatcgga gtcgacgagg
      601 ggcgggacgg gagcctgtgg ctcgccaccc acggaggcgg cctggaccgt ggaggacgcg
      661 cccgacggtg gcgcgtcgtt cgtggtccgg attccggcct gatcgcggcc ttcagcgtac
      721 tcgtgaagtc attgtgaagg cacgtcgggt aggcgtacct tgatatgcta cccccagtgc
      781 ctgccccatg atcggccttc acctcgccca gtaccgcatc gacgccgagc tcggccgcgg
      841 cggcatgggt atcgtgtacc gcgcgacgga cgcgaaactc gggcgcacgg tcgcgctcaa
      901 ggtactgccc gcgtccgcgc tcgcgtccga ggacgatcgg gcgcgcttct tccgcgaggc
      961 ccaggcggcc gcccagctct cccacccgaa catcgcgggc gtgcaccaga tcgacgaggc
     1021 cgtccccgtc gacgaacacg gcatgaaggt gggggcgagc gacggaccgc ggcctttcat
     1081 cgccatggag ttcatcgacg gcgagacggt cgccgagcgc gcgaagcgtg ggccgttcca
     1141 gctccgggag gccgtcaccg tggcgggcca ggtggccgag gcgctcaagg ctgcgcacgg
     1201 caagggcatc gtgcaccggg acatcaagag cgccaacgtc atgctgacgg gcgatggcgt
     1261 cgcgaaggtc ctcgacttcg gtctcgcgaa gacgaaccag tcgacgatgc tcacgaggat
     1321 gggctcgacg ctggggacgg tggcctacat gagcccggag caggcgcggg gtcaggaggt
     1381 cgacgggagg tcggacctgt acagtctggg cacgatgctc tacgaaatgg tcgccggacg
     1441 gcttccgttc gcaggcgatt acgagcaggc ggttctctac ggcatcctga acgagccgcc
     1501 cgagccgctc acggcggtga ggacgggcgt gccgatggag ttggagcgga tcgtggcaaa
     1561 gctgctctcg aaagaggcgg agtaccggta ccagacggcg gccgacctga tcgcggacct
     1621 caaggcgctg gacgtttccg gaagtggcgt gtcgaggcgg tcgatgcctt cgattccggc
     1681 cgcggagggg gctgcttccg cgaagtcgag ctttccggta tgggcgtggg cgatcgcggc
     1741 gacctgcctg gtcgcgggat tcgccgcggc gtggctcatc gcgtcgcgcg gtgggcaggg
     1801 tctcgcgcag tacccgaccg tgcatgcgga aatcgctctc ggcggcgggc ggatgcccca
     1861 ggccatgagc atatcgccgg acggaacgct gctcgcgtat tccgacgcgt ccgacggggc
     1921 cgtctacgtg agagacctgg agacgggtga ggagcgcatg gttccgaaca cccgtggaat
     1981 catcgaactg gacttctcgc ccgacggcca gtggctgctg ctcgacgaac agctttccat
     2041 cctgcgcgcg tcggtgtccg gaggaagccc ggtgcgactg catgtcgtcg cgtccaacgc
     2101 cccgcattcg agctgggtcg acagcgagcg catcctgttc gacgacgcaa gcagcatctg
     2161 gatgatgtcc atttcggacg gctcggtcac gccggtcgtc acggccgact cgtcggaagg
     2221 cgagttgcgc ctgctgtggg gtcgcgtgtc acccgacggg tcgcggctgt tcgcccgcat
     2281 catccgggat ccggatacgc cgaagttcgg gatctactcg ttccccgacg gcgaggtgat
     2341 cgccaaggat gactgggatg tggtctctcc ccggtacctg gagacgggcc acctgatcct
     2401 gctggagaac tccgatctca tggttcgtcc cctcgacatc gcgtccggaa gattcctggg
     2461 tgcgccggta cgcatcgccg aggccatctc ggagacgaac tgggacctgt cgtcctgggg
     2521 tcagctggtg tacggaccga tcacg
//
LOCUS       DBNHQZ010000054         8605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2724038, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000054 DBNHQZ010000000
VERSION     DBNHQZ010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2724038"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..4877)
                     /locus_tag="AC16YU_01295"
     CDS             complement(180..4877)
                     /locus_tag="AC16YU_01295"
                     /inference="COORDINATES: protein motif:HMM:NF020337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR6063885.1"
                     /translation="MHRAVPHSAAIAGLLAALLPSPQAAAQVVINEVMASNLATVPDM
                     VDFGDFEDWIELHNAGLSPVDLGGYGLADYAAAPVTWVFPAGTVVEPGGFLLVWADGG
                     NDHPGSLHTRPWWPAGQTFTTRRHHADFRLDQDGEGVYLVDPQGALADSVTFGPQRTD
                     VSIGRFPDGSPAWRAFAEATPGAPNTGAPLADPSAAPAVTAEPAGGFFASPLQVTLDA
                     GPTHPPVHYTLDGSAPTSASPVYASPIPVAQTAVVRARAIVPGRLPGPVTTRTYFVAE
                     PRTLPVVSVSANPAWLWDRDLGIYLNSYKEREIPVGFEYFPDGEPVLSVTAGARIGGE
                     NIYRFAQKPLNVYFRSEYGAPRLDWPVFDDLPYFEYKRLYVRNGGDDWPETLLRDGLV
                     RELLKGRVGNATQAFRPAVMYLNGEYWGIHNLREKIDERWFELHHGVMPADLDHLEAD
                     GTVIAGDSTAFMALLDFARTTDLSDPAAFALVAAQVDLDDLADFVAVQSIIANPSWGH
                     NREVWRDRGSDGRWRWVIVDADRGFDMARVQDPTLQDLVDAFDLFRAALADSGFRERF
                     IRRLAVHLNATFGAERVVATVDSLRGLIAPEIPRHAGRWAAYIDSLTIDIWGKTPGIQ
                     SAEAWETDVDEIRTFAATRPDIVRGQVAELFGLDGTARLVVRSEPPDRGRVRVEGEAA
                     GEGEYFLGIPVALDATAPPGLAFVHWLERSLGTSVPLVPAGSAWRYLDAGTAPEAWAE
                     AGFDDGSWPEGAAELGYGDGDEQTVLSFGPDPLDKRITYYFRARFPAPDPVGLAGLDL
                     GLLVDDGAVVYLNGTELLREGMPPGTIDPLTRASVAVSGYGEALFHRYALPADLLVDG
                     ENVLAVEVHQVSRESSDISFDLELQARYVYGEPTERVASTAPAFDYVPTADADLVAVF
                     EPAEANLVPASIQAAMLLERARSPYYVEGEVVVAAGATLEAEAGVEVLMAPGSGIRVN
                     GRLAFFGTADLPVRVAPLYPGERWGTVCIVGGDEWSELEHVELTGSGPGAAPCPAALT
                     VVDTRVRLRAVTFDDVHLPVKADGSELEILDSVFRGVTGVGDYVEANGGRLVIEGSEF
                     VGNDLVDMDAVDIGFMQAGTVIRGNVIRDFTGFNSDGVDLGDASVDILVEGNRIHDCQ
                     DKAVSVGQGSSVVVSRNVVARCGMGVGVKDSLSTAAVVHSTFHDTGIGVAVFEKNPER
                     GGASASVVSSLFSRSATAAVQADGLSTLSVRFSLSDTDDLPGEGNLRADPRLVNPDGL
                     GAWPQVMSPAIDAGDPALPPDPDGSRADIGALPFAGYVDAPVVINEITYNASAAFDTD
                     DWVELHNPSYDPVDLVGWALRDESADPAFVFPGDAVLEGRGYLVLSRRPDLFSPLCPW
                     PGPPFGTLLTGLGGGGEGIWLYDAAGRMADSVRYGDSAPWPSLADGGGASLELVDPLL
                     DNALASSWTASVGHGTPGWRNSTFVGAEDRPGDGLPTEFALEQGFPNPFNPSTRIRYR
                     LPVRSRVELRAYDALGRLVRTLVDGDLDAGHHETVWTADDLSSGVYFVSMRARPLGGG
                     PEWRAARRAVLLK"
     gene            5035..6138
                     /locus_tag="AC16YU_01300"
     CDS             5035..6138
                     /locus_tag="AC16YU_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6063886.1"
                     /translation="MPSLLHTRRAFLRTATAGFGAALAGPAARRAAARRRDETVGFAV
                     AGLGGLSSWQILPALRKTRHARLVGVVTGTPEKGRRAVEEYGIPPDAVYSYDTMDRMA
                     DNPDIDVVYVVTPNVLHLDHTVAAARAGKHVFCEKPMAGSAADCETMISACRDAGVRL
                     AVAYRCQFEPHHRECMRIVRDGDFGPLRLVEAGLGFRIGDPAQWRLRRDLAGGGALMD
                     VGVYAIQACRYLSGEEPVRITAIETKTDPVKFAEVDETIAWHMEFPAGAVGSCTASYN
                     LSGLNHFRGYCERGSFWMDPAYSYDDLEGGTSQGRFDIEPVNHFAAEMDDFARCILED
                     RESIVPGEEGLRDLRIIEAIYESSRTGRAVDLP"
     gene            complement(6158..7339)
                     /locus_tag="AC16YU_01305"
     CDS             complement(6158..7339)
                     /locus_tag="AC16YU_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6063887.1"
                     /translation="MSGRVNALPRSVWALGIVSLLMDSSSELVHSLLPVFMVSTLGAS
                     MITVGVVEGMAEATASITRVFSGTFSDWLGRRKLPALVGYGLAALSKPLFPLASTIGW
                     VFAGRFVDRVGKGIRGAPRDALVADVTPPDRRGAAFGLRQALDSVGAFAGPMLAVVLM
                     AGFSGDLRAVLWVAVIPAVAAVAVLAAAVREPAVSPSAGPLRSPVDLRVLRALPGAFW
                     IVVGLGFVFGLARFSEAFLLLRAQSAGLAIGSVPVVLVVMNVAYAATAYPAGRAADRR
                     RPEALLAAGLVLLVAADVALAAARGPAAVFAGAAAWGVHMGMTQGLFAKLVADHVPRA
                     LRGTAFGVFHVATGLALLVASFLGGLLWDRFGAPATFLAGGGFAALALVGLVAIRRRQ
                     A"
     gene            complement(7346..7792)
                     /locus_tag="AC16YU_01310"
     CDS             complement(7346..7792)
                     /locus_tag="AC16YU_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019238.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HIR6063888.1"
                     /translation="MERRSLLDPDNLAEIERRIEAIRPDTVPLWGRMNAAQMFAHCAE
                     IQEVWLGKKLEGTPVWIRLLGPLAKGALLKDRPYRKNSPTHPQYRMEGEHDFEAEKRR
                     LLGAVRTYAAHPATEFRHPIFGRFTQQESGWATYRHLDHHLSQFGV"
     gene            7943..8368
                     /locus_tag="AC16YU_01315"
     CDS             7943..8368
                     /locus_tag="AC16YU_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6063889.1"
                     /translation="MKRKGSAVWKGGLRDGKGTVSTESGVLVDQQYSFSTRFENGIGT
                     NPEELIGAAHAGCFSMALSGQLGAAGMNPEEIRTTASVTLEKQDIGFTVTAVHLDVHA
                     RIPGADPAAFEKAAAGAKAGCPISRLLKAEITMTTHLEA"
     gene            8370..>8605
                     /locus_tag="AC16YU_01320"
     CDS             8370..>8605
                     /locus_tag="AC16YU_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6063890.1"
                     /translation="MTTRLAFRLALLLLVAASGSATAQEAPADRLAEFDRDDAPGVVL
                     AVFRGGEIVHAEAHGMASLEWGVPLTTEMAFNIAS"
BASE COUNT         1342 a         3131 c         3004 g         1128 t
ORIGIN      
        1 acggccgccc cttcggcgag tggggcgacg cgctggtcga acgtccggtg gacctccaca
       61 tcgcccggtt caggtggtca tccgaccgcc cgagagagca gaacgtgccg gtcctggccg
      121 tgtacctgga cggcgagatc ttccaccggg tgtccaactg acgcccggtc cgggagcgcc
      181 tatttgagga gcaccgcccg cctcgccgct cgccactccg gaccgccgcc cagcggccgg
      241 gcccgcatcg agacgaagta cacgccgctc gagaggtcgt cggcggtcca cacggtttcg
      301 tggtgccccg cgtccaggtc tccgtcgacg agcgtccgga ccaggcgtcc cagcgcgtcg
      361 taggcccgga gctcgacccg gctccggacg ggcagccggt agcggatccg ggtggacgga
      421 ttgaagggat tggggaatcc ctgctccagg gcgaactcgg tcggcagccc gtcgcccggc
      481 cggtcttccg ctcccacgaa cgtcgagttc cgccacccgg gcgtcccgtg cccgacggac
      541 gcggtccaac tcgaggccag cgcgttgtcc agcagcggat cgacgagctc gagcgacgcg
      601 ccgccgccgt cggccagcga gggccagggc gccgagtcgc cgtaccggac ggagtccgcc
      661 atgcgaccgg ccgcgtcgta gagccagatc ccctctccgc ccccgcccag gccggtcagc
      721 agcgtgccga acgggggccc tggccacgga cacagcggac tgaagaggtc cgggcgtcgg
      781 ctcagcacca ggtaccccct gccctcgagg accgcatcgc ccggaaagac gaacgccgga
      841 tcggccgact cgtcgcggag tgcccatccg acgaggtcca ccggatcgta ggacgggttg
      901 tgcagctcga cccagtcgtc cgtgtcgaag gcggccgacg cgttgtacgt gatctcgttg
      961 atcaccaccg gcgcgtcgac gtagcccgcg aacggcagcg cgccgatgtc ggcgcgcgag
     1021 ccgtccggat cgggcggaag cgcggggtcg ccggcgtcga tggccggaga catgacctgc
     1081 ggccaggccc cgagcccgtc gggattcacg aggcggggat ccgcccggag atttccctcc
     1141 ccaggcaggt cgtcggtgtc cgaaagcgag aagcggaccg acagggtgga tagcccgtcg
     1201 gcctgcacgg cggccgtcgc ggagcgcgag aacaggctgc tgacgaccga agcgctcgcc
     1261 ccgccgcgct ccgggttctt ctcgaagacc gcgaccccga tccccgtatc gtggaaggtc
     1321 gagtgcacca cggcggccgt cgagaggctg tccttcacgc cgacccccat gccgcaccgg
     1381 gcgacgacgt tccgcgagac gacgaccgac gagccctgtc ccaccgagac cgccttgtcc
     1441 tggcagtcgt ggatgcggtt gccctcgacc aggatgtcga cggacgcgtc gccgaggtcc
     1501 acgccgtcgg agttgaaccc cgtgaagtcg cggatcacgt tgccccggat caccgtcccg
     1561 gcctgcatga agccgatgtc gacggcgtcc atgtccacga ggtcgttccc cacgaactcc
     1621 gatccctcga tgacgaggcg tccgccgttg gcctcgacgt aatcgccgac ccccgtgacc
     1681 ccacgaaaga cggaatccag gatttcgagc tcggacccgt ccgccttcac cggcaggtgc
     1741 acgtcgtcga acgtcaccgc gcgcagccgg acgcgggtgt ccaccaccgt gagcgcggcc
     1801 gggcacggcg ccgcgcccgg tcccgacccc gtcagctcga cgtgctcgag ctccgaccac
     1861 tcgtcgccgc ccacgatgca gaccgttccc cagcgctcgc ccggatagag cggcgcgacg
     1921 cgcacgggaa gatccgccgt cccgaagaat gccaggcgtc cgttcacccg gatcccgctg
     1981 cccggcgcca tcagcacctc gaccccggcc tcggcctcca gcgtggctcc ggccgccacg
     2041 acgacttcgc cctcgacgta gtacggggaa cgggcgcgct cgagcagcat cgccgcctgg
     2101 atggaagccg ggaccaggtt ggcctccgcg ggctcgaaca cggcgaccag atccgcgtcg
     2161 gccgtgggca cgtagtcgaa cgcgggcgcg gtcgaggcca cgcgctcggt cggctcgccg
     2221 tacacgtagc gggcctggag ctccaggtcg aagctgatgt ccgagctctc ccggctcacc
     2281 tggtgcacct cgacggccag gacgttctcc ccgtccacca gcaggtcggc gggcagggcg
     2341 tagcggtgga agagcgcctc gccgtatccg gaaacggcca ccgaggcgcg tgtcagggga
     2401 tcgatcgtgc ccgggggcat cccctctcgc aggagctccg tgccgttcag gtagaccacc
     2461 gcgccgtcgt cgaccaggag gcccaggtcg agcccggcca gcccgacggg gtccggcgcc
     2521 ggaaaacggg cccggaagta gtacgtgatg cgcttgtcca ggggatccgg cccgaaggag
     2581 agcaccgtct gctcgtcgcc gtcgccgtag ccgagctccg ccgcgccctc gggccacgac
     2641 ccgtcgtcga acccggcctc cgcccacgcc tcgggagccg tgccggcatc gaggtagcgc
     2701 caggccgagc cggccggaac gaggggcacc gaggtcccga gcgagcgctc gagccagtgg
     2761 acgaaggcga ggccgggcgg ggccgtcgcg tccagcgcga ccgggatgcc caggaagtac
     2821 tccccttcgc cggcggcctc tccttccacg cgtacccggc cgcggtccgg cggttcggac
     2881 cggaccacga ggcgcgccgt cccatccagc ccgaagagct cggccacctg accgcggacg
     2941 atgtccgggc gggtcgcggc gaacgtgcgg atctcgtcca cgtccgtctc ccaggcttcc
     3001 gccgactgga tgcccggcgt cttcccccag atgtcgatcg tgagcgagtc gatgtaggcg
     3061 gcccagcggc cggcgtgccg cgggatctcg ggggcgatga gcccacgcag gctgtcgacg
     3121 gtcgccacga cgcgctccgc cccgaaggtc gcgttcaggt ggacggcgag cctgcggatg
     3181 aaccgctcgc ggaagcccga gtcggcgagc gccgcccgga acaggtcgaa ggcgtcgacg
     3241 aggtcctgga gcgtcgggtc ctggacccgc gccatgtcga aaccgcggtc cgcgtccacg
     3301 atgacccagc gccagcggcc gtccgagccc cggtcgcgcc acacctcgcg gttgtgtccc
     3361 cagctcggat tcgcgatgat cgactggacc gcgacgaaat cggcgaggtc gtcgagatcg
     3421 acctgcgccg cgaccagggc gaaggccgcg ggatcggaga gatccgtcgt ccgggcgaaa
     3481 tccagcagcg ccatgaaggc cgtggaatcg cccgcgatca ccgttccgtc ggcttccagg
     3541 tggtcgaggt ccgcgggcat gacgccgtgg tgcagctcga accagcgctc gtcgatcttc
     3601 tcgcgcaggt tgtggatgcc ccagtactcg ccgttcaggt acatgacggc ggggcggaat
     3661 gcctgcgtgg cgttgcccac ccggcccttc aggagctcgc gcacgaggcc gtcgcggagc
     3721 agcgtctccg gccagtcgtc gccgccgttg cgcacgtaga gccgcttgta ttcgaagtag
     3781 ggcaggtcgt cgaacaccgg ccagtcgagc cgcggggcgc cgtattccga ccggaaatag
     3841 acgttgagcg gcttctgcgc gaaccggtag atgttctcgc ccccgatcct cgcccccgcc
     3901 gtgaccgaga gcacgggctc gccgtccggg aagtactcga atccgacggg tatctcgcgc
     3961 tccttgtacg agttgaggta gatgcccagg tcacgatccc agagccaggc cggattcgcg
     4021 gacacggaga ccaccggaag cgtccgcggc tccgccacga agtacgtccg cgtggtcacg
     4081 ggaccgggca gccggccggg aacgatggcg cgggcgcgga ccaccgcggt ctgcgcgacc
     4141 ggaatcggcg acgcgtacac gggcgaggcg gaggtcggcg ccgagccgtc tagcgtatag
     4201 tggaccgggg gatgcgtggg ccccgcatcg agcgtgacct ggagcggcga ggcgaagaac
     4261 cctccggcgg gctcggcggt cactgccggg gcggcgctcg gatcggcgag cggcgcgccc
     4321 gtgttcgggg cgccgggtgt ggcctcggca aaggcccgcc acgcgggcga gccgtcgggg
     4381 aatcgcccga tcgaaacatc cgtgcgctgc ggtccgaacg tcaccgagtc ggcgagggct
     4441 ccctggggat cgaccaggta caccccctcc ccgtcctggt ccaggcggaa gtccgcgtgg
     4501 tgccggcggg tcgtgaaagt ctgcccggcg ggccaccagg gccgggtgtg cagggagccg
     4561 ggatggtcgt tccccccgtc ggcccagacc agcaggaagc ccccgggttc gacgacggtg
     4621 ccggcgggga acacccacgt gaccggggcg gccgcgtagt ccgcgagtcc gtacccgccc
     4681 aggtccacgg gcgagaggcc cgcgttgtgc agctcgatcc agtcctcgaa gtccccgaaa
     4741 tccaccatgt cggggaccgt ggccaggttc gaggccatca cttcgttgat cacgacctgc
     4801 gccgcggcct gcggcgacgg caggagggcg gccaggaggc ctgcgatcgc tgcgctatgg
     4861 gggacggctc gatgcatcgg caccggcaag gtacgggctt tggcgcgcag atcggagcgg
     4921 cgcggtcgcc ccgggtatcg gccgatgcgc ggcgaatctt gacggatggc ggcttgccat
     4981 cgccggctgc gtcgattaga taggtcatcc gggacagttg ccggtgacca cgcgatgcct
     5041 tccctcctcc atacccgccg cgccttcctg aggaccgcca ccgccggttt cggtgccgct
     5101 ctcgccggcc cggcagcccg gcgggccgcc gcccgccgcc gcgacgagac cgtgggattc
     5161 gccgtggccg gcctgggcgg cctctcctcc tggcagatcc tgccggccct gcgcaagacc
     5221 cgccatgcgc gcctcgtcgg cgtcgtcacg ggcacgcccg agaagggccg ccgcgcggtc
     5281 gaggagtacg gaatcccgcc ggacgccgtg tactcgtacg acaccatgga ccgcatggcc
     5341 gacaatccgg acatcgacgt ggtctacgtc gtgaccccga acgtcctgca cctggaccat
     5401 accgtcgccg ccgcccgcgc gggcaagcac gtcttctgcg agaagccgat ggccggctcg
     5461 gcggccgact gcgagaccat gatctccgca tgccgcgacg ccggcgtccg cctcgccgtc
     5521 gcctaccggt gccagttcga gccgcaccat cgggaatgca tgcggatcgt ccgggacggc
     5581 gacttcgggc cgctccgact cgtggaggcc ggcctcgggt tccgcatcgg cgacccggcg
     5641 caatggcgcc tgcgacgcga cctcgcgggc gggggcgcgc tcatggacgt gggcgtgtac
     5701 gcgatccagg cctgccgcta cctctccggc gaggaacccg tccgcatcac ggccatcgag
     5761 acgaagaccg accccgtgaa gttcgccgag gtcgacgaga ccatcgcatg gcacatggaa
     5821 ttcccggccg gcgcggtcgg ctcgtgcacg gcgtcgtaca atctctccgg actgaaccat
     5881 ttccggggct actgcgagcg gggcagcttc tggatggatc ccgcctactc gtacgacgac
     5941 ctggaagggg gaacgagcca ggggcggttc gacatcgagc cggtgaacca cttcgcggcc
     6001 gagatggacg acttcgcccg gtgcatcctc gaggaccgcg agtccatcgt gcccggcgag
     6061 gagggtctgc gcgacctgcg catcatcgag gcgatctacg agtcgtcccg cacggggcgg
     6121 gccgtggacc tgccgtgatg ccgccgtccg gcccggctca cgcctgccgc cgccggatcg
     6181 cgaccaggcc cacgagcgcg agagcggcga agccgccccc ggccaggaaa gtggccggcg
     6241 cgccgaaccg gtcccagagc aggccgccga ggaagctcgc cacgaggagc gcgaggccgg
     6301 tggccacgtg gaagacaccg aaggccgtcc cccgcagcgc gcgcggcacg tggtcggcta
     6361 ccagcttggc gaagagaccc tgggtcatgc ccatgtgcac gccccaggcg gccgcgccgg
     6421 cgaacacggc ggcaggaccc cgcgcggcgg cgagcgccac gtcggccgcg acgagaagca
     6481 cgaggcccgc cgcgagcagc gcttccggac gccgccggtc ggccgcgcgg cccgctggat
     6541 aggccgtcgc cgcgtaggcc acgttcatga ccacgagcac gaccggaacc gaaccgatcg
     6601 ccaggcccgc ggactgggcg cgcagcagca ggaacgcctc gctgaaccgc gccaggccga
     6661 acacgaatcc cagcccgacc acgatccaga acgcccccgg cagcgcgcgc aggacccgga
     6721 ggtccaccgg gctgcggagg ggcccggccg acggggagac ggccggttcg cgcacggccg
     6781 cggcgagcac cgcgacggcg gccacggcgg gaatcacggc cacccacagc accgcgcgca
     6841 ggtcgcccga gaacccggcc atgaggacga cagcgagcat cggacccgcg aacgccccca
     6901 ccgaatccag cgcctgcctc aggccgaatg cggcgccgcg ccggtcgggc ggcgtgacgt
     6961 cggcgacgag ggcgtcccgc ggcgcgccgc ggatgccctt gccgacccgg tccacgaagc
     7021 ggccggcgaa cacccagccg atcgtcgacg ccagcgggaa cagcggcttg gacagcgccg
     7081 cgagaccgta ccccacgagc gccggcagct tgcgacggcc aagccagtca ctgaacgtcc
     7141 ccgagaacac cctggtgatc gacgccgtgg cctcggccat gccctcgacc acgcccaccg
     7201 tgatcatcga cgcgcccagg gtggatacca tgaacacggg caggaggctg tgaacgagtt
     7261 ccgaggacga gtccatcagc agggacacga tcccgagcgc ccatacgctt cgcggcagcg
     7321 cgttcacccg gcccgacacg gcggttcaca ccccgaactg gctgaggtgg tggtccaggt
     7381 gccgataggt ggcccagccg gactcctgct gagtgaaccg cccgaagatc gggtgccgga
     7441 actccgtggc cgggtgcgcg gcgtacgtgc gcaccgcgcc gagcagcctc cgcttctcgg
     7501 cctcgaagtc gtgctcgccc tccatccggt actgggggtg cgtcggcgag ttcttccggt
     7561 agggccggtc cttcagaagc gcccccttcg ccagcggacc cagcaggcgt atccacacgg
     7621 gcgtcccctc gagcttcttg cccagccaca cctcctggat ctccgcgcag tgggcgaaca
     7681 tctgggcggc attcatccgg ccccagagcg gcaccgtgtc cggacgaatg gcctcgatcc
     7741 gccgctcgat ctccgcgagg ttatccgggt cgaggaggct cctgcgttcc atgacgtttc
     7801 gtcctgtacg atgatgatcg caagaaacgg gacggcgccc gcaattccga gacgggaatc
     7861 ggacaccgcg gtgccgtatt ctcgctttgc cgggccgccc cgcgcgcccg gccccacccg
     7921 aacaccattt ccggaatacg acatgaagcg caagggatcg gccgtctgga agggcggcct
     7981 tcgcgacggc aagggcacgg tttccaccga aagcggcgtg ctcgtcgacc agcagtactc
     8041 gttcagcacg cggttcgaga acggcatcgg cacgaacccc gaggaactca tcggagcggc
     8101 gcacgcgggc tgcttctcga tggccttgtc cgggcagctc ggcgccgccg gcatgaaccc
     8161 cgaggagatc cggacgaccg ccagcgtgac gctcgagaag caggacatcg gcttcaccgt
     8221 cacggccgtc cacctggacg ttcacgcgag gattcccgga gcggaccccg ccgcgttcga
     8281 gaaggccgcg gcaggcgcca aggcgggatg tccgatttcg cgcctgctca aggccgagat
     8341 cacgatgacc acgcacctcg aggcgtgaca tgacgacgcg cctcgccttc cgcctcgccc
     8401 tcctgctgct cgttgccgcc tcggggtcgg ccacggccca ggaagccccc gccgaccggc
     8461 tggcggagtt cgaccgggac gacgcccccg gcgtggttct ggccgtcttc cgcgggggcg
     8521 agatcgtgca cgcggaggcc cacggaatgg cctcgctcga atggggtgtg ccgctgacga
     8581 cggagatggc attcaacatc gcatc
//
LOCUS       DBNHQZ010000055         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_555929, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000055 DBNHQZ010000000
VERSION     DBNHQZ010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_555929"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..2796)
                     /locus_tag="AC16YU_01325"
     CDS             complement(91..2796)
                     /locus_tag="AC16YU_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6063891.1"
                     /translation="MKKLILLVAVLFAVAGTQYALQLAPQAVITIQAWSPQELADAGM
                     DPFAASGLRVVGVESLVYLVGSEASGEDVLTYEWSATSPLGVVVPTDTPDAETTTFRP
                     FETGTWTIDLTITTASGAASTSITLVAGEFVGVGSIGGAIPDAGKGQCAACHPHNTAD
                     WDGTGHAHLFELGIDGIASDHYTANCISCHVVGYDLSAENGGFDDIAAAYGWTFPAET
                     VAGNWQDILDNYPVLAQRANIQCENCHGPGSEHKGIAAGIDVSAMTGVCSVCHERGSH
                     HNKPTLWKRSGHHLGTAFATRGTRTGCADCHSSYGFIAKIDPLSNLDQQVGTGEGVEC
                     AACHDPHNDDNPAQLRTIDDVELVDGTVVTWGGVGKLCMNCHQARRTGATQIFATGHI
                     DPHHSNQVDMLAGNQVAITFGRYIPSSTHRNALEDACVSCHMYETPGTPEEGQYWLGE
                     HTFAVAADMEGMVVENVEACVRCHGEIESFEDIMAREDYDGDGAVESTMDETLGLMDE
                     VGKLLPPFGDPAIDEEAEGGLGEWPLMWKKALFNYNFVLDDGSKGFHNFQYAVGLLKV
                     TIEAMNYGILSAGSITEINDVPNDQGKKVQIVWERFGGDGASDDPVTVYAVFRQDGAA
                     VAGKAPLPRLDEVDLETTSVGTSVVVEGEVWTQVGAAEAFQMDLYSLEVPTLFDGTET
                     TFKVVAKTRSSEYVVMSDPAVGVSEDNLVPTAPMSLAADATSWDVTLTWQELDFLRNP
                     DVDYYAVYRGETEGFVPGEPIATVTDPMFFDDTVVPGNWYYYQVAAFDFSGNQGEFSG
                     EVGVAVSVGTDDLGLPTDYALHQNYPNPFNPSTTIIFDVPAASEISIVVYDLLGRQVK
                     TLVSGSIAAGRHEATFDATGLTSGLYIVRMRTGDRVFERSVVLMK"
BASE COUNT          510 a         1105 c         1076 g          551 t
ORIGIN      
        1 cccgaaacgg cgacgccccg gtctccagca aggagaccgg ggcgtccgga ccggcggccg
       61 cgtcgcggcg ccggaacgtt cagggccgga tcacttcatg agcacgacac tgcgctcgaa
      121 cacccggtcc ccggtccgca tccgcacgat gtacagaccg ctcgtcaggc cggtcgcatc
      181 gaaggtcgcc tcgtggcggc ccgcggcgat cgagcccgag accagcgtct tcacctggcg
      241 gccgagcaga tcgtagacga cgatcgaaat ctcgctcgcc gccggcacgt cgaagatgat
      301 cgtggtcgac gggttgaagg ggttcggata gttctggtgc agcgcgtaat cggtcggcag
      361 gccgaggtcg tcggtgccca ccgagacggc cactccgacc tcgcccgaga actcgccctg
      421 gttgcccgag aagtcgaagg ccgccacctg gtagtaatac cagttgcccg gcacgaccgt
      481 gtcgtcgaag aacatcgggt cggtcaccgt cgcgatcggc tcgcccggca cgaagccctc
      541 ggtctcgccc cggtacacgg cgtagtagtc aacgtccggg ttgcggagga agtcgagctc
      601 ctgccacgtg agggtcacgt cccaggacgt ggcatccgcc gcgaggctca tcggggccgt
      661 cgggaccagg ttgtcctccg agacgccgac cgcggggtcg ctcatgacga cgtactcgct
      721 cgaacgggtc ttggcgacca ccttgaaggt cgtttcggtc ccgtcgaaca gcgtcggaac
      781 ctcgaggctg tacaggtcca tctggaacgc ctcggcagcg ccgacctgcg tccagacctc
      841 gccctccacg accacgctgg ttccgaccga agtggtctcc aggtcgacct cgtcgagccg
      901 cggcagcgga gccttgcccg cgaccgccgc tccatcctgg cggaacaccg catacacggt
      961 gaccggatcg tccgacgcgc cgtcaccgcc gaagcgctcc cacacgatct ggaccttctt
     1021 gccctggtcg ttcgggacgt cgttgatctc ggtgatggag ccggccgaga ggatgccgta
     1081 gttcatcgcc tcgatcgtga ccttgagcag gcccaccgca tactggaagt tgtggaaccc
     1141 cttcgagccg tcgtcgagga cgaagttgta gttgaagagg gccttcttcc acatcagcgg
     1201 ccactcaccg aggccgccct cggcctcctc gtcgatcgcc gggtcgccga acggcggcag
     1261 gagcttgccc acctcgtcca tgagccccag cgtctcgtcc atcgtgctct cgaccgcgcc
     1321 gtccccgtca tagtcctcgc gggccatgat gtcctcgaac gactcgatct cgccatggca
     1381 gcggacgcac gcttctacgt tctcgaccac catgccctcc atgtccgcgg ccaccgcgaa
     1441 ggtgtgctcg ccgagccagt actgaccctc ttccggagtg ccgggggtct cgtacatgtg
     1501 gcacgacacg caggcatcct cgagggcgtt ccggtgcgtc gagctcggga tgtagcgccc
     1561 gaacgtgatc gcgacctggt tgccagcgag catgtcgacc tggttggagt ggtgcgggtc
     1621 gatgtggccg gtcgcgaaga tctgcgtggc ccccgttcgg cgggcctggt ggcaattcat
     1681 gcagagcttg ccgacgccgc cccacgtgac caccgttccg tccacgagct ccacgtcgtc
     1741 gatcgtgcgg agctgggccg ggttgtcgtc gttgtgcggg tcgtggcagg ccgcgcactc
     1801 gacgccttcg cccgtgccga cctgctggtc caggttcgac agcgggtcga tcttggcgat
     1861 gaagccgtag gacgagtggc agtcggcgca tccggtccgc gtcccccggg tcgcgaacgc
     1921 ggtgcccagg tggtgtccgg accgcttcca cagcgtcggc ttgttgtggt gggatccacg
     1981 ctcgtggcac acggagcaga cccccgtcat ggccgatacg tcgattcccg cggcgatgcc
     2041 cttgtgctcc gagcctggtc cgtggcagtt ctcgcactgg atgttcgcgc gctgcgccag
     2101 caccgggtag ttgtcgagaa tgtcctgcca gttgccggca acggtctcgg ccgggaaggt
     2161 ccagccgtag gcggcggcga tgtcgtcgaa tccgccgttc tcggcagaga ggtcgtatcc
     2221 gaccacgtgg cacgaaatgc agttcgccgt gtagtgatcg ctcgcgatcc catcgatccc
     2281 gagctcgaac aggtgggcat ggccggtgcc gtcccagtcc gccgtgttgt gggggtggca
     2341 cgcggcgcac tggccttttc cggcatcggg gatggcgccc ccgatggatc cgacgcccac
     2401 gaactcgccg gcgacgagcg tgatcgacgt cgacgccgcg ccggaggccg tcgtgatggt
     2461 caggtcgatg gtccaggttc cggtctcgaa gggccggaac gtggtcgtct ccgcatccgg
     2521 agtatcggtc ggcacgacga cgcccagcgg cgaggtggcc gaccactcgt aggtcagcac
     2581 gtcctctcca ctcgcttccg aaccgacgag gtacacgagg ctctccacgc ccactaccct
     2641 gaggccggag gccgcgaacg gatccatccc agcatcggcc agctcctgcg ggctccaggc
     2701 ctggatcgtg atcacggcct gcggagcgag ctgaagggcg tactgagtgc cggcgaccgc
     2761 gaaaagtacg gccacgagca gtatcagctt cttcatatgg tttgttgctc ccggtgagcg
     2821 gcaaggccgc tctccgtccg attgttcaat aattcccggc cgggactccg agcctcgcgt
     2881 tgggaggcgt tccgacgcct ccgccgggcc gacgtcgcgc gttgcgacgg gggagaggtt
     2941 accggtgctg gccggcaatg ggcatcggcg ttcatccgat aatgacgccg gacggtcccg
     3001 accgacgagc acaggcagaa ccgcacgcct tgcctcgttc ggcgcgtcac gcgtccaagt
     3061 ccgcccggta cctcgcaagg gaatgcacct acacgggagg gatccgatag ggggtcccga
     3121 aacggcgacg ccccggtctc cagcaaggag accggggcgt ccggaccggc ggccgcgtcg
     3181 cggcgccgga acgttcaggg ccggatcact tcatgagcac gacactgcgc tcgaacaccc
     3241 gg
//
LOCUS       DBNHQZ010000056         9373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1235022, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000056 DBNHQZ010000000
VERSION     DBNHQZ010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1235022"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="AC16YU_01330"
     CDS             complement(<1..1104)
                     /locus_tag="AC16YU_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6063892.1"
                     /translation="MADFCHLHCHTQYSLLDGAARIQGLVERSAELELPAIAITDHGN
                     LFGVPEFFTHAKKAGVRPILGCEFYVTPSGMADRSDRTRFHQVLLAKNEEGYRNLMRL
                     SSLSYTEGFYYKPRIDRETLRAHASGLIATTCCLQGEVPQTILRKGEEAARPVFEEYL
                     EIFGEDYFIEIQDHGIADQRKVNAVLVRWAAAYGVKMVATNDVHYVQREDHAAQDVLL
                     CLQTGRDLYDPGRMRFENDQFYLKSADEMESARGLLEPAQRAEALATTLEIADRCTFE
                     LPTGRLLMPHFPLPEAFAGDAEGYLRHLALDGARARYGELRQDVVDRIEYEVGVIRDM
                     GYAGYFLIVQDFTTAARKLGVRVGPGRGSAAGSL"
     gene            1280..1915
                     /locus_tag="AC16YU_01335"
     CDS             1280..1915
                     /locus_tag="AC16YU_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063893.1"
                     /translation="MIFVVAVDRRTMEDLMAWDAVARSLSARPAGSGGLSLVDAPGEQ
                     AERTLEGEGHAIRGADGLLRAGDRASAAALARAARGENRRITATLSEAGIPAVGFLGG
                     DRGLLRAAAVPAWLAGVVHAGSVPVIASVDAGEEGARELPLGDSVAWLAGLPGARTVF
                     LADRAASETAAQAESERKLPRDSASIERVQARGISVRISTVSELASLGREP"
     gene            2082..2255
                     /locus_tag="AC16YU_01340"
     CDS             2082..2255
                     /locus_tag="AC16YU_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HIR6063894.1"
                     /translation="MRRFSQLKSFVDALEDDFTKFFDKGNKAAGTRVRKAMQDLKKMA
                     QDIRVDVQNKKNV"
     gene            2350..3219
                     /locus_tag="AC16YU_01345"
     CDS             2350..3219
                     /locus_tag="AC16YU_01345"
                     /inference="COORDINATES: protein motif:HMM:NF022720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HIR6063895.1"
                     /translation="MMHRVLLLLAFVLVVVPARAQTAEPDTSAWVRSLTGKLSLTQAG
                     FRDWADGGVSTLAASSGIDGKAERTSGAWTRIYELRAGFGLVRQDTLAFRKAEDLIVI
                     KAEHRYAGRGFFGRFTPTIAFAARSQFWEGFDYDAANALPDGSAPKVSDFMAPASFIE
                     TIGLTNQPAPWVSMRLGVSSKQTVVAIERLRPLYSMDLDQSVRVELGLEFVTDVDKNL
                     AENIHYKSSFGAFAAFNQPESPDLIWENLLEMKVNSWLTTNFEWAMLLDRDRSDALQM
                     REVLSIGIVYHFL"
     gene            3242..5506
                     /locus_tag="AC16YU_01350"
     CDS             3242..5506
                     /locus_tag="AC16YU_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6063896.1"
                     /translation="MQLRRHIEPVGYVLVALLLWPVPLLNRLHAESSAVLALAAWGLA
                     GWAAIRAFREGTGFGPVLRRRLAMLALPLALMTVPVLWAPNCDWPHGLLLFALFPGVT
                     AVLAVAAAWALTGAGVRRPLAALLAIGLALAVLGPLYDVGLHPQFYTYNHVFGGVLGP
                     LYDEETVWRPGLFAFRGLTLLWAALAWSFGAALRAAPGSRARTVALACAAGATLLVGA
                     AYVGSGRLGFNTRERDLARALPVESVVGNMTLHLDPAIEPERRRRIESEAAYRYDRLA
                     RVLEVEPSRRVEVWVYPDAETRGRLIGARVTSIAPVWLATPQVHVLERQFADVFAHEL
                     VHAFAREFGMSVLNASPAVGLVEGLAVALEPPDGGPGPDEQVAAVRTDRTAERLAESL
                     SPWGFWGGRGAVSYTTTGSFVRYLCDAYGVDAVKDVYASAGFSRVFGRSPRELADGWM
                     EALDHLEWLPVDASHRARSRFAVPSLFERRCPHHVPATVRHRRASERAWERRDTLAAL
                     RAAQRALSADPRDPATIALALRMQLRAGDDDAARRLLTETPDSLLTATGALVAADLMA
                     IDGRAEEARERYDQVRRSLPASSREPRAVLTLRQLLAGRPDALRLLVLGGLARDPAGG
                     LARLRRQDPDAASQAAIGMTEALVRADHRGFADAFRILETVRPAGLSAAGDSAWRGVS
                     AGERNELALQRLAWLAVLGEAAGDLGASAAYADSAAGLSRALSDLVAERYWTDQAGRL
                     RHPRGSFHRPAQTP"
     gene            5503..6825
                     /locus_tag="AC16YU_01355"
     CDS             5503..6825
                     /locus_tag="AC16YU_01355"
                     /inference="COORDINATES: protein motif:HMM:NF052315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system plug protein"
                     /protein_id="HIR6063897.1"
                     /translation="MTPRATPFRSGRAGLAVAVVLVLWSAAGCASTEKERDDEDAPRR
                     TAPEPRFAARADGIGTIQLYRTGDEASLPVLPLGSGQTLTLAFDLVRQAGRPVSVYFY
                     HADRSWRRDLVPSEYLETFHRDDVLDYGPSSGTQVPYTHYRYEFPNRSIGFRLSGNYV
                     VRVTEQGMEEDVLFERPFFVSEQAAAIDLSLGNVMVAGSPIAHLQPSLSFTPPDPTYG
                     NAFDYTVCFQRNALLDDGRCTDDPSLAMAPELTYFLEPEASFGPRITEHFLDISEIRV
                     GGRVESADLESNPFRLLLEPDYIRFPGAIGVPQLNGSPVVDAAVRSLAEPSRSADYAE
                     VRFALVPEDDVPVRGGVWVLGSFNDWSRRPEHRMEWNAERRRYEGTALLKQGLYEYRY
                     GFENASLARQVAFDAPPQADALVTAFIYFSDVRVSTDRLITAVSVRGQ"
     gene            6993..>9373
                     /locus_tag="AC16YU_01360"
     CDS             6993..>9373
                     /locus_tag="AC16YU_01360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR6063898.1"
                     /translation="MTRIVPAALALVILLGGCSTTRRSAQVPAAADTTSARSRTAQEI
                     KPYRDVIKSDFAHDEGLFTVHRDKQKIWYEIPNAMLGRELLLVTRVARTADNLGFGGQ
                     KWNTLVLRWDRQDDTVLLRVVSYENVASPGDPIYEAVRNANLEPIVEAFDIAALSEDG
                     SGVVIEVSDLFTSDVPAVGMAPSRRTGYQIRRVDSDRSYIVSAKSYPENIEVRHVLTY
                     EAGQPPSNSTTGAVTVELNQSMILLPARPMQPRLCDERVGFFNVRMTDYSRDAQRAEE
                     RCYVTRWRLEPSDPEAYARGELVEPVKPITYYIDPATPVKWRPYLKQGIEDWREAFEA
                     AGFRNAIVAKDPPSPEEDPEFSPEDVRYSVIRYFPSEVQNASGPHVHDPRTGEILESD
                     INWYHNVMNLLRNWYFVQTAAADPRARGVRFDDALMGELVRFVSAHEVGHTLGLYHNF
                     IASSAVPVDSLRSPTYTSRNGTAPSIMDYARFNYVAQPGDGVTRFFAQIGPYDRWAID
                     WGYGRRFDHLGTDGETVELNRIVKEKNQDPALLFFQTAVADPRTQTEDLSDDAVRASD
                     LGIENLKRITQNLVSWTRREAEDYDDLEELYGQVLGQWNRYVGHVARSVGGVYQTAKT
                     YDQEGPVFEPVERERQERAVDWMDRQVFTTPAWMIAPDVLDRIQAYGISDRILGYQRG
                     ALEQLLDGRRLGRMLEAEALGRPEAYTVAELMGDLHAILWREVTESAVVDDPFRRNLQ
                     RVHVEALGELLDPDPVPTNAFQGAGVNLKATDVPALARAELERILSDVGRALAR"
BASE COUNT         1312 a         3308 c         3363 g         1390 t
ORIGIN      
        1 caggctgccg gccgccgagc cgcggccggg ccccacgcgc acgcccagct tccgggcggc
       61 ggtcgtgaaa tcctggacga tcaggaagta gccggcatag cccatgtcgc ggatcacgcc
      121 gacctcgtac tcgatccggt cgaccacgtc ctgccggagc tcgccgtagc gggcgcgcgc
      181 gccgtcgagc gcgaggtgcc gcaggtatcc ctccgcgtcc ccggcgaagg cctcgggcag
      241 cgggaagtgc ggcatgagca ggcgtcccgt gggcagctcg aacgtgcacc ggtccgcgat
      301 ttcgagggtc gtcgcgaggg cctccgcgcg ctgggcgggc tcgagaagcc cgcgcgccga
      361 ctccatctcg tcggccgatt tcaggtagaa ctggtcgttc tcgaagcgca tgcggccggg
      421 gtcgtacagg tcccggcccg tctgcaggca cagcaggacg tcctgggccg cgtggtcctc
      481 gcgctggacg tagtgcacgt cgttcgtcgc gaccatcttc acgccgtacg ccgccgccca
      541 acggacgagc accgcgttca ccttgcgctg gtccgctatg ccgtggtcct ggatctcgat
      601 gaagtagtcc tcgccgaaga tctccaggta ctcctcgaat acggggcggg cggcctcctc
      661 gcccttcctg agaatcgtct ggggcacctc gccctggagg cagcacgtcg tcgcgatgag
      721 gcccgacgcg tgggcccgga gggtttcccg gtcgatccgc ggcttgtagt agaagccctc
      781 cgtgtacgag agcgaggaca gccgcatcag gttgcggtag ccttcctcgt tcttcgccag
      841 cagcacctgg tggaaccggg tccggtccga gcggtcggcc attccagagg gcgtcacgta
      901 gaactcgcac cccaggatcg gccggacgcc cgccttcttc gcatgcgtga agaattccgg
      961 gacgccgaag agattgccgt ggtccgttat ggcgatggcg gggagctcga gctccgcgct
     1021 gcgttcaacg aggccctgga tgcgcgcggc gccgtcgagc agggaatact gcgtgtggca
     1081 gtgcaggtgg cagaagtcgg ccatggagcc ggtcggtgcg tgcgttccgg ggagcgggcg
     1141 agcctgaagt aagtcgacgc cggtgccgcg gggcaagcct tgtggatatc tcggcgaccg
     1201 ggcgagcggc tctcggatga acctccccgg ccggccgcgg ttcgctcccg ggaacacttc
     1261 gtccggcggg gcccgtcgca tgattttcgt cgtcgccgtc gatcggcgga ccatggagga
     1321 tctgatggcc tgggacgccg tggcgcggtc cctctcggcc cgccccgccg gctccggcgg
     1381 actgagcctg gtggacgcgc cgggcgagca ggcggagcgg acgctcgagg gcgagggtca
     1441 tgcgatccgc ggcgccgacg ggctgctccg cgccggggat cgggcgtcgg ccgcggcgct
     1501 cgcccgggcc gcccgcggcg agaaccggcg catcacggcg acgctctccg aggcgggcat
     1561 cccggcagtc ggcttcttgg gcggggatcg cgggctcctg cgcgcggcgg cggtgccggc
     1621 ctggctggcg ggcgtggttc acgcgggctc ggtgcccgtc atcgcgtcgg tagatgccgg
     1681 agaggaggga gcgcgggagc tgccgctcgg ggattcggtc gcctggctgg cgggccttcc
     1741 gggagcgcga accgtcttcc tggcggaccg tgcggcctca gaaaccgccg cgcaggccga
     1801 atcggagcgg aaattgcccc gcgactcggc atcgatcgag cgcgtgcagg cgcggggcat
     1861 aagcgttcgt atttcaacgg tttccgagct cgcatcactc ggccgcgagc catgacggac
     1921 atcccggcgg gcgccggaat ggctcaaatc agcgcggaaa cgcagaaact tccttgacac
     1981 ccccgggacg tccgcgtacc ttcgcaaaac ccagaggcac gggcacccat tcaaaccgtc
     2041 cgcgtcagcg ctaaccccgt tcaaacagga ggaaatccgg catgcgcaga ttctcacagc
     2101 tcaagtcgtt cgtcgacgca ctcgaagacg acttcacgaa gttcttcgac aagggcaaca
     2161 aggccgccgg cacgcgcgtc cgcaaggcca tgcaggacct caagaagatg gcccaggaca
     2221 tccgcgtgga cgtccagaac aagaagaacg tctgaggacg cacccgtcca cggaattcgg
     2281 aaaggctgcg tccaggcttg ggcgcggcct ttcgtctttt cagccgccgc gcaccgattc
     2341 gagccctcga tgatgcatcg cgtactgctc cttctcgctt tcgtcctcgt cgtcgtcccc
     2401 gcccgggcgc agacggccga acccgacacg tccgcctggg tgcgctccct caccggaaag
     2461 ctctcgctca cgcaggcggg cttccgcgac tgggcggacg gcggcgtgag cacgctcgcg
     2521 gcctcgagcg gcatcgacgg caaggcggag cggacgagcg gggcgtggac ccggatctac
     2581 gaactgcgag ccggcttcgg actcgtgcgg caggacaccc tggcgttccg caaggcggag
     2641 gacctcatcg tcatcaaggc cgagcaccgg tacgcgggcc ggggattctt cggccgcttc
     2701 accccgacga tcgccttcgc cgcgcggtcc cagttctggg agggcttcga ctacgacgcg
     2761 gccaacgcgc ttccggacgg cagcgctccc aaggtgtccg acttcatggc gccggcgagc
     2821 ttcatcgaga cgatcggtct cacgaaccag cccgcgccct gggtctcgat gcgcctcggc
     2881 gtctcgtcca agcagacggt cgtcgcgatc gagcgccttc ggccgctcta ttccatggac
     2941 ctggaccaga gcgtccgcgt ggagctcggc ctggagttcg tgacggacgt ggacaagaac
     3001 ctggccgaga acatccacta caagtcctcc ttcggggcgt tcgccgcgtt caaccagccc
     3061 gagtcgccgg acctgatctg ggagaacctg ctcgagatga aggtgaacag ctggctcacg
     3121 acgaacttcg agtgggccat gctcctggac cgggaccgtt ccgacgcgct gcagatgcgg
     3181 gaagtgctct cgatcgggat cgtgtaccac ttcctgtagc cccgggcggg cggagggtgc
     3241 gatgcagctc cggcgccaca tcgagccggt cggctacgtc ctcgtggcgc tcttgctctg
     3301 gccggtgccg ctcctgaacc ggctgcacgc cgagtcgagc gccgtcctgg cgctcgcggc
     3361 gtgggggctg gcgggctggg cggcgatccg cgcgttccgg gaagggacgg gcttcggccc
     3421 ggtcctgcgg cggcgcctcg ccatgctcgc cctgccgctc gccctgatga ccgtgcccgt
     3481 gctctgggcg ccgaactgcg actggcccca cggactgctc ctgttcgcgc tctttccggg
     3541 cgtcacggcg gtcctcgcgg tggcggccgc gtgggcgctc accggggcgg gggtgaggcg
     3601 gccgctggcc gcgctcctgg cgatcggcct ggccctcgcc gttctcggcc ccctgtacga
     3661 cgtgggcctc caccctcagt tctacacgta caaccacgtg ttcgggggcg tgctcggccc
     3721 cctgtacgac gaggaaaccg tgtggcgccc gggactcttc gcgttccggg ggcttacgct
     3781 cctctgggcc gcgctcgcct ggagtttcgg cgccgcgctc cgggcggcgc cgggaagccg
     3841 ggcgcggacc gtggccctcg cgtgcgccgc cggggcgacg ctcctggtcg gggccgcgta
     3901 cgtcgggtcc gggcgcctgg gcttcaacac gagggagcgc gacctcgccc gcgccctgcc
     3961 ggtcgaatcg gtcgtgggca acatgacgct gcacctggat cccgccatcg agcccgagcg
     4021 ccggcgccgc atcgagagcg aggcggcgta ccggtacgat cggctcgccc gggtcctgga
     4081 ggtcgagcct tcgcggcgcg tcgaggtgtg ggtgtacccg gacgcggaga cgcgcgggcg
     4141 cctcatcggc gcccgggtga cgagcatcgc cccggtgtgg ctcgccacgc cgcaggtcca
     4201 cgtgctcgag aggcagttcg cggacgtgtt cgcgcacgag ctcgtccacg cgttcgcgcg
     4261 cgaattcggc atgtcggtcc tgaacgcctc gcccgctgtg ggcctcgtgg agggtctggc
     4321 cgtggcgctc gagccgccgg acggcggacc gggtccggac gagcaggtgg cggccgtccg
     4381 caccgaccgc acggcggaac gcctggccga atccctgtcg ccgtggggct tctggggcgg
     4441 tcgcggggcc gtgtcgtaca cgacaaccgg ctcttttgtc cggtatctgt gcgacgcgta
     4501 cggggtcgat gccgtgaagg acgtctacgc gagcgccggc ttcagccgcg tgttcgggcg
     4561 ctccccgcgc gagctggccg acgggtggat ggaggcgctg gaccacctcg agtggctccc
     4621 ggtcgatgcc tcgcaccggg cgcgctcccg cttcgccgtg ccgtcgctct tcgagcggcg
     4681 ctgcccgcac cacgttccgg ccacggtgcg gcaccggagg gcgtcggaac gggcctggga
     4741 gcgccgcgac accctcgccg ccctgcgcgc ggcgcagcgg gcgctctcgg ccgatccccg
     4801 cgacccggcg accatcgcgc tcgcgcttcg catgcagctg cgcgcgggcg acgacgacgc
     4861 ggcgcggcgg ctgctcacgg agactcccga ttcgctcctg acggccaccg gggcgctcgt
     4921 ggcggcggac ctcatggcga tcgacgggcg cgcggaggag gcccgcgagc gctacgacca
     4981 ggtccgccgc tcgctgcccg cctcgtcgcg ggagccccgg gccgtgctca cgctgcggca
     5041 gctgctcgcc ggccggcccg acgcgctgcg cctcctggtc ctcggcgggc tcgcgcgcga
     5101 tcccgcgggc ggcctggcga ggcttcgccg ccaggacccg gacgcggcct cgcaggcggc
     5161 catcggcatg accgaggccc tggtccgggc cgatcaccgc gggttcgcgg atgcgtttcg
     5221 catcctggag acggtccgcc cggcggggct ttcggccgcg ggcgattccg cgtggcgcgg
     5281 ggtttcggcg ggcgagcgga acgaactcgc gctccagcgc ctggcgtggc tcgcggtgct
     5341 cggcgaggcg gccggggacc tgggcgcgtc cgctgcgtat gccgattccg cggcgggcct
     5401 gtcccgcgcg ctcagcgacc tcgtggccga gcgctactgg acggaccagg ccgggaggct
     5461 ccggcacccg cgcggatcgt tccatcggcc agcccagacg ccatgactcc ccgagcgacg
     5521 cctttccgat ccggccgcgc cggcctcgcg gtcgctgtcg tgctcgtcct gtggtcggcg
     5581 gcgggctgcg cttcgaccga aaaggagcgc gacgatgagg acgccccgcg ccgcacggcc
     5641 cccgagcccc gcttcgccgc gcgggcagac ggcatcggca ccatccagct gtaccgcacg
     5701 ggcgacgagg cctcgctgcc cgtcctcccg ctcggctccg ggcagacgct gaccctcgcc
     5761 ttcgacctgg tccggcaggc gggccggccg gtgtccgtgt acttctacca cgccgaccgg
     5821 tcgtggcgcc gcgacctggt gccgtcggag tacctcgaga cgttccaccg ggacgacgtg
     5881 ctcgactacg gaccctcctc cggcacccag gttccgtaca cgcactaccg ctacgaattc
     5941 ccgaaccgct cgatcggctt ccggctgagc ggcaactacg tggtccgggt cacggagcag
     6001 gggatggagg aggacgtcct gttcgagcgc ccgttcttcg tctcggagca ggcggccgcc
     6061 atcgatctct cgctcgggaa cgtgatggtg gcggggtcgc ccatagcgca cctgcagccg
     6121 tcgctgtcgt tcacgccccc ggacccgacg tacggcaacg cgttcgacta cacggtgtgc
     6181 ttccagcgca acgcgctcct cgacgacggg cgctgcacgg acgatccgtc gctggccatg
     6241 gccccggagc tcacgtactt cctggagccg gaggcgtcgt tcggtccgcg catcacggag
     6301 cacttcctgg acatctccga gatccgggtc ggcggccgcg tggagtcggc cgacctcgag
     6361 tcgaatccct tccggctgct gctggagccg gactacatcc gcttcccggg cgccatcggc
     6421 gtcccgcagc tgaacggcag ccccgtggtg gacgccgccg tccggagcct ggccgagccg
     6481 tcgcgctcgg ccgactacgc cgaggtgcgc ttcgcgctcg tcccggagga cgacgtgccc
     6541 gtgcgcggcg gcgtgtgggt gctcggctcc ttcaacgact ggtcgcgccg cccggagcac
     6601 cgcatggagt ggaacgccga gcggcgccgg tacgagggga cggcgctcct gaagcagggc
     6661 ctgtacgagt atcgctacgg cttcgagaac gcatccctcg cccggcaggt cgccttcgac
     6721 gcacccccgc aggcggacgc cctggtcacg gccttcatct acttctcgga cgtgcgggtg
     6781 agcacggacc ggctgatcac ggcggtgtcg gtaaggggcc agtaggcgga ggggtcggtc
     6841 agggcccggg tggcggcggg gcgggggctg tcggagcgac ggccggcggc ttgccgatgc
     6901 ggcgatgcgc gggttgccga agcggcgggg cgggggtatt ctatccgcgc ccggaccccg
     6961 acgtccgcat gcagtcgagc aagcgcacgc ccatgacccg catcgtaccg gccgccctgg
     7021 ccctcgtgat ccttctcggc ggatgcagca cgacccgccg ctcggcccag gtccccgcgg
     7081 ccgcggacac gacttccgcc cgctcccgca ccgcgcagga gatcaagccg tacagggacg
     7141 tgatcaagag cgacttcgcc cacgacgagg gcctcttcac cgtccaccgc gacaagcaga
     7201 aaatctggta cgagatcccg aacgcgatgc tgggccgcga gctgctgctc gtcacccggg
     7261 tcgcccggac ggccgacaac ctgggcttcg gcggccagaa gtggaacacg ctcgtgctcc
     7321 ggtgggaccg gcaggacgac acggtgctgc tgcgggtcgt cagctacgag aacgtggcca
     7381 gtcccgggga tccgatctac gaggcggtgc gcaacgcgaa cctggagccg atcgtcgagg
     7441 cgttcgatat cgcggccctg tcggaggacg gctcgggagt cgtgatcgag gtgtcggacc
     7501 tcttcacctc cgacgtgccg gccgtcggca tggcgccgtc gcgccgtacg ggctaccaga
     7561 tccggcgcgt ggattcggac cgctcgtaca tcgtctcggc gaagagctac cccgagaaca
     7621 tcgaggtgcg gcacgtgctc acgtacgagg cgggtcagcc gccgtcgaac tcgacgacgg
     7681 gcgcggtcac ggtggagctg aaccagtcca tgatactgct cccggcccgc ccgatgcagc
     7741 cgcgcctgtg cgacgagcgc gtgggcttct tcaacgtgcg catgacggac tacagccggg
     7801 acgcgcagcg ggccgaggag cgctgctacg tcacgcgctg gcgcctggag ccatccgacc
     7861 cggaggccta cgcgcggggc gagctcgtgg agcccgtgaa acccataacg tactacatcg
     7921 acccggcgac gcccgtgaaa tggcgtccct acctgaagca gggcatcgag gactggcgcg
     7981 aagccttcga ggcggccggc ttccgcaacg cgatcgtcgc gaaggatccg cccagccccg
     8041 aggaggaccc cgagttcagc ccggaggacg tgcgctactc ggtgatccgc tacttcccgt
     8101 ccgaggtcca gaacgcgtcg gggccgcacg tgcacgaccc gcggacgggc gagatcctgg
     8161 agagcgacat caactggtac cacaacgtga tgaacctgct ccggaactgg tacttcgtgc
     8221 agaccgcggc ggcggacccg agggcgcgcg gcgtccggtt cgacgacgcg ctcatgggcg
     8281 agctggtccg gttcgtgtcg gcgcacgagg tggggcacac cctcgggctg taccacaact
     8341 tcatcgcctc gagcgcggtc cccgtcgact cgctgcgctc gcccacgtac acgtcccgga
     8401 acggcacggc gccgtccatc atggactacg cgcgcttcaa ctacgtggcg cagccgggcg
     8461 acggggtcac gcgcttcttc gcgcagatcg gcccctacga ccgctgggcc atcgactggg
     8521 gctacggccg ccggttcgac cacctgggga cggacggcga gaccgtagag ctgaaccgca
     8581 tcgtgaagga gaagaaccag gaccccgcgc tcctcttctt ccagaccgcg gtcgcggacc
     8641 cgcgcacgca gaccgaggac ctgtccgacg acgccgtccg cgcgagcgac ctgggcatcg
     8701 agaacctgaa gcgcatcacg cagaacctgg tctcgtggac gcggcgcgag gccgaggact
     8761 acgacgacct ggaggagctc tacggccagg tcctgggcca gtggaaccgc tacgtggggc
     8821 acgtggcccg ctcggtgggc ggcgtgtacc agacggcgaa gacctacgac caggagggcc
     8881 ccgtcttcga gcccgtggaa agggagcggc aggagcgggc ggtggactgg atggaccggc
     8941 aggtgttcac gacgcccgcg tggatgatcg cgcccgacgt gctggaccgc atccaggcct
     9001 acggcatctc ggaccggatc ctgggctacc agcggggcgc cctcgagcag ctgctcgacg
     9061 gccggcggct gggccgcatg ctggaggcgg aagcgctcgg ccgcccggag gcctacaccg
     9121 tggcggagct catgggcgac ctgcacgcga tcctgtggcg cgaggtcacc gagagcgcgg
     9181 tcgtggacga ccccttccgc cgcaacctgc agcgcgtgca cgtcgaggcc ctcggcgagc
     9241 tcctggaccc tgaccccgtc ccgacgaacg cgttccaggg ggcgggcgtg aacctgaagg
     9301 ccaccgacgt cccggcgctc gcgcgggcgg agctggagcg gatcctgtcg gacgtgggcc
     9361 gggcgctggc ccg
//
LOCUS       DBNHQZ010000057         4604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3131192, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000057 DBNHQZ010000000
VERSION     DBNHQZ010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3131192"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="AC16YU_01365"
     CDS             complement(<1..567)
                     /locus_tag="AC16YU_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6063899.1"
                     /translation="MSAKHTPVVRALLALALMLAIGALFNADGAFFRWSVHRDMLRQV
                     SVFGILACGMTVVIITAGIDLSVSSLLALSAVAFAWLTLPAGLPAAFAVPAVLAAGAL
                     LGSLSGALVARMRIQPFIVTLAMMVFARGLAKVLAGGRKITNYVALPDGTSEVVDLPA
                     VFGMIDSRVLGGNVSIVTVIFLACVLFTW"
     gene            complement(564..2195)
                     /locus_tag="AC16YU_01370"
     CDS             complement(564..2195)
                     /locus_tag="AC16YU_01370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR6063900.1"
                     /translation="MFSISVEAGASIQRLTGMDIDQDRDHMGRTEHPVVAMRGIRKSF
                     GPVEVLHGVDLTVRPGEVHVLAGENGAGKSTLIRILSGVYRDFGGSVELSGREVRFAR
                     PADATRAGIATIHQELALVPSLDVADNLFLGRELRGTLGGVDFRAQRRVAADVLAELG
                     LEVEPDTPVGALPIATRQMIEIARALRGDARVLIFDEPTSALGEREVEVLFGRIRDLR
                     GRGAGILYITHRMEEIYRLADRITVLRDGSLVGTAEAADLPPDELVRWMVGRELEVAG
                     SSESHVRLEVALAVEDLSVRRDDGSGLPAVDHVSLQLLRGEIVGLAGLQGSGASEVLQ
                     ALFGTLGRRAWGSVELGGAPFRMESPAASIRSGMVLLSNDRNALGLAPEAGVTQSMSL
                     ASLERFAGPFGWIRRPEERNEVADFVRDFRISAPSLDAPVRTLSGGNQQKVLLARCLM
                     TRPDVLLLDEPTRGIDVGAKADLYALMRGWTADGAAILLITSEMDELLNLADRILVMH
                     RGRIAASFLGRGARRDAILAAAMGHDMDGPPSDTR"
     gene            2200..3393
                     /locus_tag="AC16YU_01375"
     CDS             2200..3393
                     /locus_tag="AC16YU_01375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HIR6063901.1"
                     /translation="MNKHASILALCALLAAPTLAQGVDGTLFDLAHIKEGVRSKRVSS
                     YDRTGGNNDRFENIADGETRTLFDVEGAGMINHIWITIAPPPETLSRNDIILRMYWDG
                     SDEPCVETPIGPFFGQGWNEAYDWSSLPLATGPANGRAMVSYFVMPFADGARIEVQND
                     TGRKIDAFYYYVDYVETDELPPDAGRFHAWYNHELTEALAGGENEWGVLGPQAKNVTG
                     DGNYVFADIEGKGHFVGVNYYVHSPGPIWYGEGDDMFFIDGEAWPSSLHGTGTEDYFN
                     TAWVPKTLFSHPYYGYARVNDDFGWLGRTHVYRFHIADPVYFDRSLRAGIEHGHDNAL
                     TLDLASVAYWYQREPHKPFPELPDREARAPKPFTEHWQIHLWRDAWRREMGGDSTLWG
                     NERRD"
     gene            3655..4275
                     /locus_tag="AC16YU_01380"
     CDS             3655..4275
                     /locus_tag="AC16YU_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HIR6063902.1"
                     /translation="MRTHIPGTRHLLSLIFVLLLAHLAAQNARAHCDALDGPVVRDAR
                     TALEQRDLAPVLKWILPEDEAEVRRAFELALVVREKGPESRELADLHFFGTVVRLHRA
                     SEGEPYTGLKPAGTDPGPAVSGADEALREGDASSLVRLVTDEVDRGLRERFEHAHQAL
                     GHSGESVEAGRAYVAAYVEFVHYAKRLLEDASHDATHGIGTAPAGH"
     gene            complement(4313..>4604)
                     /locus_tag="AC16YU_01385"
     CDS             complement(4313..>4604)
                     /locus_tag="AC16YU_01385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6063903.1"
                     /translation="ITVVDRYQGKGVGSMLLAALSRAARREGIETFVGYVESDRRRLL
                     RFLASLPGASIRAGDGVAEVHFPVLDDPDLWPDTPYGRLGRSVLTVEPVNDR"
BASE COUNT          777 a         1611 c         1597 g          619 t
ORIGIN      
        1 ccacgtgaag aggacgcacg cgaggaagat cacggtgacg atcgaaacgt tgccgccgag
       61 cacccggctg tcgatcatcc cgaagacggc gggcaggtcc acgacctcgc tcgtgccgtc
      121 ggggagcgcc acgtagttcg tgatcttgcg cccgcccgcg agcaccttcg cgaggccgcg
      181 cgcgaagacc atcatggcga gcgtcacgat gaagggctgg atgcgcatcc gcgccacgag
      241 ggccccggag agcgagccga gcagggcgcc cgcggccagg acggccggga cggcgaacgc
      301 ggcgggaagg cccgcgggga gcgtgagcca ggcgaaggcg accgcggaca gcgcgagcag
      361 gctgctcacg gagaggtcga tgcccgccgt gatgatgacc accgtcatgc cgcacgccag
      421 gatgccgaag accgacacct ggcggagcat gtcgcggtgc acgctccacc ggaagaaggc
      481 gccgtcggcg ttgaagagcg cgccgatggc cagcatcagg gccagcgcga ggagcgcgcg
      541 gacgaccggg gtatgcttcg cgctcatcgg gtgtcggagg gtggaccgtc catatcgtga
      601 cccatggcgg cggccaggat ggcgtcgcgc cgcgcgcctc gcccgaggaa cgaggcggcg
      661 atccgtccgc ggtgcatcac gaggatacgg tcggcaaggt tcagcaattc gtccatctcg
      721 gaggtgatga ggagaatggc cgcgccgtcg gccgtccacc cgcgcatcag cgcgtagagg
      781 tcggccttcg ccccgacgtc gatcccccgc gtgggctcgt cgagcagcag gacgtccggc
      841 cgggtcatga ggcagcgggc gaggaggacc ttctgctggt tgccgcctga cagcgtgcgg
      901 acgggggcgt ccagcgacgg cgcggagatc ctgaaatcgc ggacgaaatc cgcgacctcg
      961 ttccgttcct ccgggcgccg gatccacccg aagggacccg cgaaccgctc gagcgaggcc
     1021 aggctcatcg actgcgtgac gccggcctcc ggtgcgaggc cgagcgcatt gcggtcattc
     1081 gacagcagca ccatgccgct ccggatcgac gcggcggggc tctccatgcg gaacggcgcg
     1141 ccgccgagct cgaccgatcc ccacgccctg cggcccaggg tgccgaagag cgcctgcagc
     1201 acctcgctcg cccccgatcc ctgcaggccg gcgagcccga cgatctcgcc gcggaggagc
     1261 tgcagcgaga catggtccac cgccgggagg cccgatccgt cgtcgcggcg gaccgagaga
     1321 tcctccacgg cgagagccac ttcgagccgg acgtgcgact ccgacgaccc ggcgacctcc
     1381 agctcccgcc cgaccatcca gcgcaccagt tcgtccggcg ggaggtcggc ggcttcggcg
     1441 gttccgacga gcgaaccgtc gcgcagcaca gtgatccggt ccgcgagccg gtagatctct
     1501 tccatccggt gcgtgatgta caggattccg gcgccgcggc cgcggagatc gcggatgcgc
     1561 ccgaacagga cctcgacctc gcgctcgccg agcgcgctcg tcggctcgtc aaagatgagc
     1621 accctcgcgt cgccccggag ggcgcgggcg atctcgatca tctgccgggt agcgatgggg
     1681 agcgcgccga ccggagtgtc cggctcgacc tcgagtccga gctcggccag gacgtccgcg
     1741 gcgacccggc gctgcgcccg gaaatccacc cctccgagcg tgccgcgcag ctcgcgcccc
     1801 aggaacaggt tgtcggccac gtccaggctg gggacgagcg cgagctcctg gtggatggtc
     1861 gcgatgccgg cccgggtcgc gtccgcaggg cgcgcgaagc gcacctcccg gccggagagc
     1921 tcgacgctcc cgccgaagtc acggtacacg ccggagagga tgcggatcag agtgcttttg
     1981 ccggccccgt tctcgcccgc cagcacgtgc acctctcccg gacggaccgt aagatccacg
     2041 ccgtgcagca cctccacggg cccgaaggac ttccggatgc cgcgcatcgc gacgaccggg
     2101 tgctccgttc ggcccatatg gtcccgatcc tggtcgatgt ccatgcccgt gagccgctgt
     2161 atcgaagcgc cggcctcaac gctaatggaa aacacggcca tgaacaagca cgcctcgatc
     2221 ctcgcgctct gcgccctcct cgccgccccg accctcgcgc agggcgtgga cggcacgctt
     2281 ttcgatctcg cgcacatcaa ggagggggtc cgctcgaagc gggtctcgag ctacgaccgc
     2341 acgggcggca acaacgaccg gttcgagaac atcgcggacg gcgaaacccg cacgctcttc
     2401 gacgtggagg gcgccggcat gatcaaccac atctggatca cgatcgctcc gccgcccgag
     2461 acgctgagcc gcaacgacat catcctgcgc atgtactggg acggctcgga cgagccgtgc
     2521 gtcgagacgc cgatcggccc gtttttcggc cagggctgga acgaggccta cgactggtcc
     2581 tcgctgccgc tggccacggg cccggcgaac ggccgggcga tggtctcgta cttcgtgatg
     2641 cccttcgcgg acggggcgcg catcgaggtc cagaacgaca cgggccggaa gatcgacgcg
     2701 ttctactact acgtggacta cgtcgagacg gacgagctcc cgcccgacgc cggccgcttc
     2761 cacgcctggt acaaccacga gctcaccgag gccctggcgg gcggcgagaa cgagtggggc
     2821 gtcctgggac cgcaggcgaa gaacgtgacc ggcgacggca actacgtgtt cgccgacatc
     2881 gaggggaagg ggcacttcgt gggcgtcaac tactacgtcc actcgcccgg gcccatctgg
     2941 tatggggagg gggacgacat gttcttcatc gacggcgagg cgtggccctc gtcgctccac
     3001 gggacgggga cggaggacta cttcaacacg gcctgggtgc cgaagacgct cttcagccac
     3061 ccgtactacg gctatgcgcg cgtgaacgac gacttcgggt ggctcggccg tacgcacgtg
     3121 taccgcttcc acatcgcgga ccccgtctac ttcgaccgct cgctccgggc gggcatcgag
     3181 cacggccacg acaacgccct gacgctcgac ctcgcgtcgg tcgcctactg gtaccagcgg
     3241 gagccccaca agccgttccc cgagcttccc gaccgcgagg cccgcgcccc gaagccgttc
     3301 acggagcact ggcagatcca cctgtggcgc gacgcgtggc gccgcgagat ggggggtgac
     3361 tcgacgctct ggggcaacga gcggagggat tgagacgggg gtcgcggccc ggggcagggc
     3421 aggataatgg tgaagaaatg gtgaaccgaa cacggatgcg gaaaccctcg accatactcc
     3481 aggctgtttc aggcagtgtc actcacgaaa cggatcccgt ggaatccacc atggaatcac
     3541 tgaccaccgg agcggtggac tcacaagcgg cgtgacgccc gagtgcctgg tgatccatgc
     3601 gctcgacacc gggaaggacg tttcgtaaac cacccgcaag ccctgaggct agccatgaga
     3661 acgcacatcc caggtaccag acatctgctc tcgctcatct tcgtcctgct cctcgcgcac
     3721 ctcgcagccc agaacgcccg ggcgcactgc gacgccctgg acggcccggt cgttcgcgac
     3781 gcgcggacgg ccctggagca gcgggacctc gcgccggtcc taaagtggat actcccggag
     3841 gacgaagccg aagtgcggcg cgccttcgaa ctggccctcg tcgttcggga gaagggccct
     3901 gaatcccgcg agctggccga cctgcatttc tttggcacgg tcgtccgcct gcaccgcgcg
     3961 agcgagggcg agccgtacac cggcctgaag cccgcgggca cggacccggg tcccgcggtt
     4021 tcgggtgcgg acgaggccct gcgcgagggc gacgcgagct ccctggtcag gctcgtcacg
     4081 gacgaggtgg accgtgggct tcgcgagcga ttcgagcatg cgcaccaagc gctcggccac
     4141 tccggcgaga gcgtcgaggc cggcagggcg tacgtcgcgg cctacgtcga attcgtgcac
     4201 tacgccaagc ggcttctgga ggacgcctcg cacgacgcga cgcacgggat cgggacagcc
     4261 cccgccgggc attgacttcg ggccggccgg aaccgcggcg agcagtgatc ggctatcggt
     4321 cgttcaccgg ttccacggtg agcacgctcc gcccgagccg cccgtacggc gtgtctggcc
     4381 acaggtccgg atcgtcgagc acggggaagt gcacctcggc cacgccgtcg ccggcccgga
     4441 tcgaggcgcc gggcagggag gcgagaaagc ggagcaggcg tcgccggtcc gactcgacgt
     4501 atcccacgaa ggtctcgatg ccctcgcggc gggcggcccg gctcagcgcg gccagcagca
     4561 tcgagcccac gcccttgccc tggtagcggt ccacgaccgt gatg
//
LOCUS       DBNHQZ010000058         2589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_421370, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000058 DBNHQZ010000000
VERSION     DBNHQZ010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_421370"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="AC16YU_01390"
     CDS             complement(<1..1003)
                     /locus_tag="AC16YU_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063904.1"
                     /translation="MSLAAASGATARQSAARAQSATPAEIQFVFGSDTSTSGFDLDDP
                     TTLYNAGGFDLYASPAGTGVRVMEDAFRGQNRDWFGDPVRLTWWMQAGSLYGTAENTN
                     VPLGSTMSPYRMRTQQGEAIERHGDEFTFHYHTWVWSDYDGDGKWYWNQALSFEDSRE
                     DFDLTMAQMLLEEDTFPVSFRSGWHFMDDAWQARLDGLVPFSMHNDWPHVRNDTVEPT
                     DNIYDWSRSPSDFVPFHPATDDYQVPGDGRSWNVRSIYFGRATDALLRQVFESAQAGT
                     GQVVCIWSHLAETTFVDDLQAVLDRIAVVAADYPDVPYRFPTAVEAMQRWLGTEDATP
                     "
     gene            complement(1215..1853)
                     /locus_tag="AC16YU_01395"
     CDS             complement(1215..1853)
                     /locus_tag="AC16YU_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063905.1"
                     /translation="MKPVPILLSVLLAGGLFVGCDDGIAPDADVAAKQEACTTIQSGE
                     LLTSAGDVITPGYDQWGYNYQAMMFNGYYCDAYRDAAWCQPYVEDHLSMKWNAAWLSN
                     VDCDGDDKLDRHFGFASYIGSGAWLTNHMYGTYEGEAGELCDWDYFVKIVAAPDDATL
                     QGGNWFAADGTLIGPAIWGEFALIQEVANDPCDSFEDGVLFKNEAPTGFGFY"
BASE COUNT          412 a          869 c          872 g          436 t
ORIGIN      
        1 gcggcgtggc gtcctccgtt cccagccacc gctgcatcgc ctcgacggcc gtcgggaatc
       61 ggtacggcac gtccgggtag tcggccgcca cgaccgcgat gcggtcgagc acggcctgca
      121 ggtcgtccac gaacgtggtc tcggccaggt ggctccagat gcagaccacc tgtccggtac
      181 ccgcctgggc cgactcgaaa acctggcgca gcagcgcatc ggtggcccgg ccgaaataga
      241 tcgagcggac gttccacgag cggccgtcgc ccggcacctg gtagtcgtcg gtcgccgggt
      301 ggaacgggac gaagtccgac ggcgagcggc tccagtcgta gatgttgtcg gtcggctcga
      361 ccgtgtcgtt gcgcacgtgc ggccagtcgt tgtgcatgct gaacgggacg agcccgtcga
      421 ggcgcgcctg ccacgcgtcg tccatgaagt gccagccgct ccggaacgag accgggaacg
      481 tgtcctcctc gagcagcatc tgggccatcg tgaggtcgaa gtcctcgcgc gagtcctcga
      541 acgagagggc ctggttccag taccacttcc cgtccccgtc gtagtccgac cacacccacg
      601 tgtggtagtg gaacgtgaac tcgtcgccgt gacgctcgat cgcctcgccc tgctgcgtcc
      661 gcatgcggta cggcgacatc gtgctgccga gcgggacgtt cgtgttttcg gccgtcccgt
      721 acaggctccc cgcctgcatc caccaggtga ggcggaccgg atcgccgaac cagtcccggt
      781 tctggcctcg gaacgcgtcc tccatcacgc gcaccccggt gcccgcgggc gaggcgtaga
      841 ggtcgaagcc cccggcgttg tagagcgtgg tcggatcgtc caggtcgaag cccgacgtcg
      901 acgtgtccga gccgaagacg aactggattt cggcgggggt ggcggactgc gcgcgggccg
      961 cggactgtcg ggcggtcgcg cccgaggcgg ccgcgaggct caagagcgcc aggacggcaa
     1021 tccggagggc ggcgggtatg gtcttcggca gatggatcgc acggtcgccg gggaagaacc
     1081 cgacgcaatc agcggcacga gtcggctcaa agatacggac cgggccgccg cgacgacccg
     1141 gtccgcaacg ttcttcgccc taagtgcacg gggggtgccc attgagatag gggagctgct
     1201 gagcgaccgc cggtctagta gaagccgaaa ccggtggggg cctcgttctt gaacagcacg
     1261 ccgtcctcga acgagtcgca cggatcgttg gcgacctcct ggatgagcgc gaactcgccc
     1321 cagatcgcag gtccgatgag cgtgccgtcg gcagcaaacc agtttccgcc ctgaagtgtc
     1381 gcatcgtcgg gcgccgccac gatcttgacg aagtagtccc agtcgcacag ttcgcctgct
     1441 tcgccttcgt acgtcccgta catgtggttc gtcagccagg cgcccgagcc gatgtagctc
     1501 gcgaagccga aatgccggtc gagcttgtcg tcgccgtcgc agtcgacgtt gctcaaccag
     1561 gcggcgttcc acttcatgct caagtggtct tccacatagg gctggcacca ggcggcatcg
     1621 cggtaggcgt cgcagtagta cccattgaac atcatcgcct ggtagttgta gccccactgg
     1681 tcgtaaccgg gcgtgatgac gtcgcccgcg ctggtcagca gctcgccgct ctggatggtg
     1741 gtgcaggcct cctgcttcgc cgcgacgtcg gcatcggggg cgatgccgtc gtcgcagccc
     1801 acgaacaggc cgccggccag gaggacagag aggaggatcg ggacaggttt catgattgca
     1861 tgtgtggttg gtggtacggg tctggaatgc tctctgtgcg attgtagtgg gtctgggtgg
     1921 ctgcttgacc catgctgccc cctttgggag tgatggagaa ctcttaaggc cgtcaggcgg
     1981 acgaagcttc tgcaacttcc ggagtgcggt gtgaatctcg acttcacccg tcgcgaaggt
     2041 ttccaccctc gctctcgttg acaatacggg ctgatccccg gggatcgggg cgcggtttcg
     2101 aatcagaatc gaaactcgac ggcgctgcgg cacgctgagg tgcattggaa accgtgatcc
     2161 gcgggcctca ggccacgagt acggaccggg gcgtcgcggc cgcccgtcct cgtccacgtg
     2221 cgcgaggacc gtgtggactg cgcgcagttc ctcctcagcc agctcttcgg ttcggcctct
     2281 gacaccgagc cggttgaagc ctcgtgacag cccggcgcgc cgcccccccg aaccttcccc
     2341 cgcgcactgt cgtaagtggc gttttgcgga caccggtgat cccccaccca gcgtccgcgc
     2401 ccgagcatga cgcacccgga atccccgcgt cccgaatcgg acgtgacgac cagcaccgtt
     2461 ggcagtggcg gggtcgcccc ccgcgagccc cacggatccc ggtccccgga gggcggcgag
     2521 gccacacgcg agcctcgcac cagcgctgcc cgcgagcctc gcaccccggc cgacgacctc
     2581 gactaccgc
//
LOCUS       DBNHQZ010000059         8345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3402823, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000059 DBNHQZ010000000
VERSION     DBNHQZ010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3402823"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="AC16YU_01400"
     CDS             complement(<1..1973)
                     /locus_tag="AC16YU_01400"
                     /inference="COORDINATES: protein motif:HMM:NF039503.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5695 domain-containing protein"
                     /protein_id="HIR6063906.1"
                     /translation="MKPAGRAFSRDTRSRTRTDRPRRRPAARWLGAAAAVLLAGAAAA
                     PAVAQGGLDLAGGFLGFDASALRLELVADSQTAAALRPAAEPSFDYTPGDWLERRRGD
                     GYHHLGDLTLRIRSGGPWRDVSTAVARRSVLTLPASGSVLAAADLSPTLPADLPLSVN
                     RRWERHGDDLLLRFEIRNTSAGEVEIGALGIPMIFNSILHGRGLDEAHAVASFHDPYI
                     GRDAGYVQVVRLDGHGPVLLVVPEPGTPLEAWNPLLSDSTRRGVTFEGFYEWMAHSAA
                     YAETEWLEAEPWNAPSSAVLGPGEARTYGLRFLVAPDIRSVEETLASAGRPVAVGIPG
                     YVLPTDIDGALFVRSASGIVATDVEPVGAVEIEPADGPDGWRAFRVRGRTWGRVRVSI
                     TYADGAVQTVHYRVIDPAADVVDALGRFLTTEQWFERPGDPFGRSPGIITWDAEAGRQ
                     VTEDHRTWIAGLGDEGGSGSWLAAVAKQLLRPERDELARIQRFVDETLWGGLQYTDAH
                     PDAPYGVRKSLFYWEPDAMPPGTYSPDVPYGGWSSWSREHAESVGRSYNYPHVAAAYW
                     VLYRLARTREGLVTSHGWEWYLDRAWRTGVAMTRHAPHYAQFGQMEGTVFVHILEDLA
                     REGWDDAAAEFEGAMRARADHWLSLGYPFGSEMP"
     gene            2061..4058
                     /locus_tag="AC16YU_01405"
     CDS             2061..4058
                     /locus_tag="AC16YU_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR6063907.1"
                     /translation="MKRAGTGSAVAVLLLAAALPLATRAQLTPDEAIGMMARGINIGN
                     TLEPPTEGGWNNGPVQEYYFDDYVAAGFTCVRIPIRWDNHTGQSPPYAVAASWMDRVE
                     QIVDWGLERGLFIIINGHHEDWLKNGYADPALRARYDSIWTQISERFSQKSDRLLFEI
                     INEPYGMTRQQVDELNARILGIIRETNPTRIVVYSGNEWSGSATMMAAAIPDDDYLMA
                     YFHSYDPWSFAGEGQGTWGSASDRAGLAQQFQAVANWSASAGIPVMISEFGAVHQADY
                     NSRMAHYAAYVDHSLTHGIAFQSWDDGGNFGVYERGTRTWNEVKDILVKSFVDGPTLV
                     GHEVRGDSVVVLSWTNRSVAYDGLRVEVQDGDGDFAEAALLPGDATAYEDTTVGPGQT
                     VTYRVVAFEDGHPDRVSYPHRAFVAPWRRSGFHGGPLPIPGTIEAEDFDVGGEGLTYH
                     DADPVNTGGAYRPGEGVDIETRLEGGFHVGWVEAGEWLEYAVDVASGGTYELTAHVAA
                     TASGGIFRFEIGSARSSLFIAPGTGSWQTTAPVTKSITLVAGEQIMRVSILSTVSAFN
                     LDRFVLVPAGSTAIEELPGPGSLAIYPNPVGAELTLALDREPGPDVHAELVNLLGQVV
                     RRIGLHGRTTRLTTEGLPGGLYFVRILEPRGIATGRTVVVL"
     gene            4120..5226
                     /locus_tag="AC16YU_01410"
     CDS             4120..5226
                     /locus_tag="AC16YU_01410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HIR6063908.1"
                     /translation="MDAPLVSIHALATRVPASKYPQETIGEILRGELEPGGRGDRLMR
                     KIYRSSGIETRHSVIEDHRNGAGGSLFFDGESGAFLSPTTGERNDEYVRKALPLFRDT
                     ARDALERSGFAPGEVTHVVTVSCTGFMAPGPDYHIVRDLGLDASVERYHVGFMGCYAA
                     FPALRMAAAFCRSDPRAVVLVVCLELCTLHLEPSEEIDNIIASSVFADGAAGAVVSGR
                     RPRGPGVALVASTTRLAPDSEADMAWRIGDRGFEMTLSTYVPKILGAHIGDLVDGILG
                     DSGRTRRDVSHWWVHPGGRSILDQVEQALDLPGGALAASRAVLARYGNMSSATILFVV
                     EEALRSGSASPGDLAYAMAFGPGLTVESALFRVV"
     gene            5240..5929
                     /locus_tag="AC16YU_01415"
     CDS             5240..5929
                     /locus_tag="AC16YU_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6063909.1"
                     /translation="MPTPLLGDRRTDLAEIMDQADCDEARLDRTYRHFRLLNRLIAGW
                     GTVYGRWIRPRLLRGPATLVDIGCGGADVPRLLTRWAGRDGLDLHATGIDPDERAIAH
                     ARRQDDMELIACDSLALASTGRTFDFVLSNHLVHHLDEAGRAALLRDSERLATCAAIH
                     NDIRRDDLGYAAFWLFGLAFPGSFIRPDGLTSIRRSLTRAELEAVLPLTWRCVPMAPY
                     RLLAVHEKPGC"
     gene            5910..7055
                     /locus_tag="AC16YU_01420"
     CDS             5910..7055
                     /locus_tag="AC16YU_01420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6063910.1"
                     /translation="MRNRAADRTDVLVVGAGPVGLDLACRLVLGGLSVRVLERRTEPT
                     NHSRSIGNHPPALEALGEVGVARRLVAAGVAVREGVAMRESGPVGRLTFERCPGPYRF
                     VLAVPQWRTEAVLEDRLRELDRGALVRGVTVSGIREDAGRVSAQSPDGRRFEADCLVG
                     CDGWDSIVRTSLGIGFPGKAYPDRYMMGDFADSTDFGPVAMIFLARDGLVESFPLPEG
                     RRRWVVRLRETVENATADEVVRRVRARTGVEVDPATASMVSGFGVHRYLATTMARGRV
                     LLAGDAAHVVSPIGGQGMNLGWLDSRAAARAIAGAADDPARADDLFRAWSSARRRAAR
                     AAGRRAEFNMALGRPSPLRPLKLAAVRWMLTPPLARVFARRFTMRGL"
     gene            complement(7063..>8345)
                     /locus_tag="AC16YU_01425"
     CDS             complement(7063..>8345)
                     /locus_tag="AC16YU_01425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6063911.1"
                     /translation="QAQQPPAELTTEEDHALMMARLGIDSLRPGANGMDPEARDYANY
                     DEALANPYPHLPQLLRANDRTPVTTADAWWSVRRPELVELFEREIYGRVPADAPAVRW
                     EVTGSRDSVLSGVAVTIQDLVGRVDHSAYPAIEVNIRAELAVPTAAAGPVPVVVQFGW
                     IGPFPWRRPGQQGPAGPTWKDLVVARGWGYAVLVPNSVQADNGAGLTRGVIGLANHGR
                     PRAPDDWGALRAWAWGASRLLDHLESDPRTDARRVALEGHSRYGKAAVVAMAFDRRFG
                     AVFASSSGAGGLSLYRRDYGEIVENLTGSGEYHWMAGNFLKYGGPLTWDDLPVDAHGL
                     VALCAPRPVFISSGDVGDAWVDARGMFLAGAHAGPVYELLGARGLGTMDFPPLETGLM
                     EGEIAFRQHAEGHTDGPNWPVFLDFAARYFDPAR"
BASE COUNT         1154 a         2977 c         3007 g         1207 t
ORIGIN      
        1 ggcatctcgc tcccgaacgg gtacccgaga gagagccagt gatcggcccg ggcgcgcatg
       61 gcgccctcga actcggcggc cgcgtcgtcc cagccctcgc gcgcgagatc ctcgaggatg
      121 tggacgaaga cggtgccctc catctggccg aactgggcgt agtggggcgc gtgccgcgtc
      181 atggcgacgc ccgtgcgcca cgcgcggtcc aggtaccact cccacccgtg gctcgtcacg
      241 agtccctcgc gggtccgcgc cagccggtac aggacccagt acgccgccgc gacgtgcggg
      301 tagttgtagg accggccgac gctctccgcg tgctcccggc tccagctcga ccagccccca
      361 tagggcacat ccggggaata cgtgcccggg ggcatcgcgt ccggctccca gtagaacagg
      421 ctcttgcgca cgccgtaagg cgcgtccgga tgggcgtccg tgtactgcag gccgccccac
      481 agcgtctcgt ccacgaatcg ctggatcctg gccagctcgt cccgctccgg gcgcagcagc
      541 tgcttcgcca cggcagccag ccaggagccc gagcctccct cgtccccgag ccccgcgatc
      601 catgtgcggt ggtcctcggt cacctggcgg ccggcctccg cgtcccacgt gatgatgccc
      661 gggctgcggc cgaaggggtc gcccggtcgc tcgaaccact gctccgtcgt gagaaaccgc
      721 ccgagggcgt ccaccacgtc cgcggccgga tcgatcaccc ggtaatgcac ggtctgcacg
      781 gcgccgtccg cgtacgtgat cgacacgcgc acccggcccc acgtccggcc gcgcacccgg
      841 aaggcgcgcc agccgtccgg gccgtccgcg ggttcgattt ccacggcccc gacgggctcc
      901 acgtccgtcg cgacgattcc ggaggcggat cgtacgaaaa gcgccccgtc gatatcggtc
      961 ggaagcacgt atccgggaat gcccacggcc acgggccggc ccgcggacgc cagcgtctcc
     1021 tcgaccgagc gaatgtccgg ggcgaccagg aagcggaggc cgtaggtccg ggcctctccg
     1081 ggaccgagga ccgcggacga gggcgcgttc cacggctcgg cctccagcca ctcggtctcc
     1141 gcgtacgccg cgctgtgggc catccactcg taaaagccct cgaaggtgac gccacgcctc
     1201 gtggaatccg agaggagggg gttccacgcc tcgagcggcg tgcccggctc gggcaccacg
     1261 agcaggacgg ggccgtggcc gtcgagcctc acgacctgca cgtacccggc gtcgcgcccg
     1321 atgtacggat cgtggaacga ggcgacggcg tgcgcctcgt cgagcccgcg cccgtgcagg
     1381 atgctgttga agatcatcgg gatgccgagg gcgccgatct cgacctcgcc ggccgaggtg
     1441 ttacggatct cgaaccgcag gagcaggtcg tcgccgtgcc tctcccagcg gcggttcacc
     1501 gagagcggca ggtcggcggg cagggtgggc gagaggtcgg cggccgcgag cacgcttccc
     1561 gatgcgggaa gcgtcagcac ggaccgccgg gccacggccg tggacacgtc gcgccacggt
     1621 ccccccgacc ggatccggag cgtcaggtcg cccaggtgat ggtacccgtc gccgcgccgc
     1681 cgctcgagcc agtcgcccgg agtgtagtcg aacgagggct ccgccgcggg tcggagcgcg
     1741 gcggcggtct gcgagtccgc gacgagctcc agccgcagcg cggaggcatc gaagccgagg
     1801 aatccgccgg cgagatcgag tcccccctgg gccacggccg gcgccgcggc cgctccggcg
     1861 aggaggaccg ccgcggcggc gccgagccac cgcgccgccg gccgacgccg gggccggtcc
     1921 gtgcgggtgc ggcttcgcgt atcgcgtgaa aacgcccttc cggcgggctt catgccttgt
     1981 atccggttgt cgtcaaaata ccttgctccc ccggacgatc gcggagccgc gcgaccgccg
     2041 acgccaactt acggtacgcc atgaaacgag caggaaccgg aagcgccgtc gccgtgcttc
     2101 tcctcgccgc cgcgctgccg ctcgcgacgc gggctcagct gacccccgac gaggccatcg
     2161 gcatgatggc gcgcggcatc aacatcggca acacgctgga gccgccgacg gaagggggct
     2221 ggaataacgg tccggtccag gaatactact tcgacgacta cgtggccgcc ggcttcacgt
     2281 gcgtccggat tccgatccgc tgggacaacc atacgggcca gtcaccgccg tacgccgtcg
     2341 ccgcgtcgtg gatggaccgg gtggagcaga tcgtggactg ggggctcgag cgggggctct
     2401 tcatcatcat caacgggcac cacgaggact ggctgaagaa cggctacgcg gacccggcgc
     2461 tccgggcgcg ctacgacagc atctggacgc agatctccga gcgcttcagc cagaagtcgg
     2521 accggctgct cttcgagatc atcaacgagc catacggcat gacccgccag caggtcgacg
     2581 agctgaacgc ccgcatcctg ggcatcatcc gggagacgaa ccccacccgc atcgtcgtct
     2641 actcgggcaa cgagtggtcg ggctcggcca cgatgatggc cgcggcgatt cccgacgacg
     2701 actacctgat ggcgtacttc cactcgtacg atccgtggtc gttcgcgggc gaggggcagg
     2761 gcacgtgggg atccgcctcc gaccgcgccg ggctcgccca gcagttccag gcagtggcca
     2821 actggtcggc gtcggcgggc attcccgtca tgatcagcga attcggcgcc gtccaccagg
     2881 cggattacaa ttcgcggatg gcgcactacg cggcgtacgt ggatcattcg ctcacgcacg
     2941 ggatcgcgtt ccagtcatgg gacgacggcg gcaatttcgg ggtctacgag cgcggcacgc
     3001 gcacctggaa cgaggtgaag gacatcctgg tgaagtcgtt cgtggacggc ccgacgctcg
     3061 tcgggcacga ggtgcgcggc gactcggtcg tcgtgctctc gtggacgaac cgctccgtgg
     3121 cctacgacgg cctgcgggtc gaggtgcagg acggggacgg cgacttcgcg gaggcggcac
     3181 tgctgccggg cgacgcgacc gcgtacgagg acaccaccgt ggggcccggc cagacggtca
     3241 cctaccgcgt ggtggcgttc gaggacgggc acccggaccg ggtttcctac ccgcaccggg
     3301 cgttcgtggc gccgtggcgg cggtcgggct tccacggagg accgcttccg attccgggca
     3361 cgatcgaggc cgaggacttc gacgtggggg gcgagggtct cacgtaccac gacgcggacc
     3421 cggtgaacac cggaggcgcc tatcgccccg gggagggcgt cgacatcgaa acccgcctgg
     3481 agggcggctt tcacgtgggc tgggtcgagg ccggggagtg gctggagtac gcggtggacg
     3541 tggcctctgg cggcacctac gagctcaccg cccacgtggc ggcgacggcc tccggcggca
     3601 ttttccggtt cgagatcggg tccgcgagat ccagcctgtt catcgcgccg ggcaccggca
     3661 gctggcagac caccgcgccg gtaacgaagt ccatcacgct cgtcgccgga gagcagatca
     3721 tgcgggtctc gattctgagc accgtgtccg ccttcaacct ggaccgattc gttctcgtcc
     3781 cggccggatc gacggcgatc gaggagctgc ccgggccggg ctctctcgcc atctacccca
     3841 acccggtggg cgccgagctg acgctcgctc tcgatcgcga gcccggcccg gatgttcacg
     3901 cagagctcgt caacctgctc gggcaggtcg tccgccgcat cggtcttcac ggccggacga
     3961 cccggctcac gaccgagggc ctgccgggcg ggctctactt cgtgcgcatc ctggagccgc
     4021 gaggcatcgc caccgggcgg acggtggtcg tcctctgacg gtctccgccg ggcaccgtgt
     4081 cggggccagc gtacattgcg gcgtcgactg ctcccgacca tggacgcacc cctagtcagc
     4141 atacacgccc tggccacccg ggtcccggca tcgaagtacc cccaggagac catcggcgag
     4201 atcctgcgcg gcgagctcga gccggggggc cgcggcgacc ggctcatgcg gaagatttac
     4261 cgcagctccg gcatcgagac gcggcattcg gtgatcgagg accaccgcaa cggggcgggg
     4321 ggaagcctct tcttcgacgg ggaatccgga gcgttcctgt cgccgacgac gggcgagcgg
     4381 aacgacgagt atgtccgaaa ggcccttccc ctgttccggg acacggccag ggacgccctc
     4441 gagcgctcgg gattcgcccc cggggaggtc acgcacgtcg tcacggtgtc gtgcacgggc
     4501 ttcatggcgc cgggtccgga ctaccacatc gtgcgtgatc tcgggctcga cgcctccgtc
     4561 gagcgctatc acgtcgggtt catggggtgc tacgcggcgt ttcccgcgct ccggatggcc
     4621 gccgcgttct gccgctcgga ccctcgtgcc gtggtcctgg tcgtgtgcct ggagctgtgc
     4681 acgctgcacc tggagccctc cgaggagatc gacaacatca tcgcgtcctc cgtcttcgcg
     4741 gacggcgccg ccggcgccgt ggtcagcggc cggaggccgc ggggaccggg cgtggcgctc
     4801 gtcgcctcga ccacgcggct cgcccccgac tcggaggcgg acatggcctg gcggatcggt
     4861 gaccgcgggt tcgagatgac gctgtccacc tacgtgccga agatcctcgg cgcccacatc
     4921 ggggacctgg tggacgggat cctgggcgac tcgggacgca cccggcggga cgtctcgcac
     4981 tggtgggtcc atcccggcgg acgttccatc ctggatcagg tggagcaggc cctggacctt
     5041 ccgggcgggg cgctcgccgc gagccgcgcg gtcctggcgc ggtacggcaa catgtcgagc
     5101 gcgacgatcc tgttcgtcgt cgaggaggcg ctccggagcg gctccgcgtc gcccggcgac
     5161 ctcgcgtacg cgatggcctt cggtcccggg ctgaccgtgg agagcgcgct cttccgcgtc
     5221 gtgtagcccg gacccgccca tgcccacccc gcttctgggc gaccgccgca cggatctcgc
     5281 cgagatcatg gaccaggccg actgcgacga ggcccggctg gaccgtacgt accggcactt
     5341 ccgcctcctg aaccggctca tcgccggatg gggaaccgtc tacgggcgct ggatccggcc
     5401 ccggctgctc cgtggcccgg ccaccctcgt cgacatcggg tgcggcgggg cggacgtgcc
     5461 ccggctgctg acccggtggg ccggccggga cgggctggac ctccacgcga cggggatcga
     5521 ccctgacgag cgggcgatcg cccatgcacg gcgccaggac gacatggagc tcatcgcgtg
     5581 cgattccctc gccctggcct ccacgggccg gaccttcgac ttcgtcctct cgaatcacct
     5641 ggtccaccat ctggacgagg cgggccgtgc ggcgctcctc cgcgactcgg agcgactggc
     5701 cacgtgcgcg gcgatccaca acgacatccg gcgcgacgac ctgggctacg cggcgttctg
     5761 gctcttcggg ctggcgttcc caggctcgtt catccggccg gacgggctca cgtccatccg
     5821 gcgttcctta acgcgggccg agctcgaggc cgtgctgccc ctcacctggc ggtgcgtccc
     5881 gatggccccc taccgtctcc tcgcggtcca tgagaaaccg ggctgctgac cgcacggacg
     5941 tgctcgtcgt gggcgcgggc cccgtgggcc tggacctcgc ctgccgcctg gtcctcggcg
     6001 ggctttcggt ccgcgttctc gagcgacgca ctgaacccac gaaccattcc cgctccatcg
     6061 gcaaccaccc gccggcgctc gaggccctgg gcgaggtcgg cgtggctcgc agactggtgg
     6121 cggccggggt ggccgtgcgg gaaggcgtcg ccatgcggga gtccggtccc gtggggcggc
     6181 tcacgttcga gcggtgtcct ggcccgtacc ggttcgtgct cgccgtgccc caatggcgga
     6241 cggaagcggt gctcgaggac cggctccgcg agctggatcg gggcgccctg gtgcggggcg
     6301 tcacggtgtc gggcatccgg gaggacgccg gtcgggtctc ggcgcagtcc ccggacggcc
     6361 gccgcttcga ggcggactgt ctcgtggggt gcgacgggtg ggacagcatc gtccggacgt
     6421 ctctcgggat cggcttcccc gggaaagcct atccggaccg gtacatgatg ggggacttcg
     6481 cggactcgac ggatttcggg ccggtggcca tgatcttcct ggcccgcgac ggcctggtcg
     6541 agtcgttccc gctgccggag gggcggcggc gatgggtggt acgccttcgg gaaaccgtgg
     6601 agaatgccac ggcggacgag gtcgttcggc gggtccgggc ccgcaccggg gtcgaggtgg
     6661 atccggccac cgcgagcatg gtttcgggct tcggcgtgca ccggtacctg gccacgacga
     6721 tggctcgggg acgggtgctc ctggcgggag acgcggccca cgtcgtcagc ccgatcggcg
     6781 ggcaggggat gaacctcggc tggctcgact cgcgtgcggc ggcccgtgcg atcgcgggcg
     6841 ccgcggacga tccggcgcgg gccgacgatc tcttccgggc ctggtcgtcg gcgaggcgcc
     6901 gggcggcgcg cgcggccggt cggcgggcgg agttcaacat ggccctggga cggccgtcgc
     6961 cgcttcgtcc gctcaagctg gcggccgtcc ggtggatgct gacgccgccg ctggcccgag
     7021 tcttcgcccg ccgcttcacg atgcgcgggc tctgaacggc cgctaccggg cgggatcgaa
     7081 gtatcgggcg gcgaaatcca ggaatacggg ccagttgggg ccgtccgtat ggccctcggc
     7141 gtgctgccgg aacgcgatct cgccctccat gagcccggtc tcgaggggcg ggaagtccat
     7201 ggtgccgagg ccgcgggccc cgagcagctc gtacaccggg ccggcatggg cgccggcgag
     7261 gaacatgccc cgggcgtcca cccacgcgtc gcccacgtcg ccgctcgaaa tgaacacggg
     7321 ccgcggcgcg cagagcgcca ccaggccgtg ggcgtctacc ggtagatcgt cccacgtgag
     7381 cgggccgccg tacttgagga agttgcccgc catccagtgg tactctccgg aaccggtgag
     7441 attctccacg atctcgccgt agtcgcggcg gtacaggctc aggccgccgg cgccggacga
     7501 gctggcgaac acggcgccga accgccggtc gaaggccatg gcgaccacgg cggccttgcc
     7561 gtaacgcgag tggccctcga gcgcgacgcg gcgggcgtcc gtgcgcggat cgctctccag
     7621 gtggtccagg agccggctcg cgccccaggc ccacgcccgc agcgcgcccc agtcgtccgg
     7681 cgcgcgaggc cggccgtgat tggccaggcc gatcacgccg cgcgtgagcc ccgcgccgtt
     7741 gtccgcctgg acgctgttcg gaacgaggac ggcgtagccc cagccacggg ccaccacgag
     7801 gtccttccag gtcggccccg ccgggccctg ctgccccggc cgtcgccagg ggaacggccc
     7861 gatccatccg aactggacca cgacgggcac gggtccggcg gcggccgtcg gcacggccag
     7921 ctccgcccgg atgttcacct cgatcgcggg gtaggccgaa tggtccaccc ggccgacgag
     7981 atcctggatc gtgacggcga ctcccgagag caccgagtcc cgggagccgg tcacctccca
     8041 gcggacggcg ggcgcgtcgg ccggcacgcg cccgtagatc tcgcgctcga acagctccac
     8101 gagctcgggt cgccgcacgg accaccacgc gtccgcggtc gtgaccggtg tccggtcgtt
     8161 ggcgcgcagg agctgcggca ggtgcgggta gggattcgcg agcgcttcgt cgtagttcgc
     8221 gtagtcgcgc gcctcggggt ccatgccgtt cgcgccggga cggagcgagt cgatcccgag
     8281 gcgagccatc atcagcgcgt ggtcctcctc ggtcgtgagc tcggccggcg gctgctgggc
     8341 ctgcg
//
LOCUS       DBNHQZ010000060         9070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1913195, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000060 DBNHQZ010000000
VERSION     DBNHQZ010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1913195"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1408
                     /gene="fabF"
                     /locus_tag="AC16YU_01430"
     CDS             149..1408
                     /gene="fabF"
                     /locus_tag="AC16YU_01430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR6063912.1"
                     /translation="MDRPMRRVVVTGMGAVTPIGLTVDAFWDAMMAGISGAAPIAAFD
                     AEAFDTKFACELKEFDPGDYLDRKIARRLDRFSQLALIAADQAVADSGIEPDRLTEDE
                     KDRVAVIFGSGIGGMKVFHEQVVTYHESGPKRLSPFFIPMLIPDIAAGQISIRHGFRG
                     PNYCVVSACATGNNNLGDAFMLIQAGHADVAVSGGAEASVTEMGVGGFNALKALSTRN
                     DDPTRASRPFDATRDGFVLGEGAGALILEELGHARARGARIYAEVAGIGMSADAYHIT
                     APDPDGRGARAAMKAALLHAGIAPTDVDYLNMHGTSTPLGDIAETNAIKAVFGDHAYR
                     MNVSSTKSMTGHLLGAAGAVEAIAAILAVRNDRVPPTINFEHADADCDLNYTFNAPED
                     RPVRVALSNAFGFGGHNTTVAFRKFED"
     gene            complement(1445..1738)
                     /locus_tag="AC16YU_01435"
     CDS             complement(1445..1738)
                     /locus_tag="AC16YU_01435"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6063913.1"
                     /translation="MSRRKTPASLGDLIPEVVDGLGLTDRLEEARIEAEWRAMAGPRI
                     GRVTRRVRLHGDRLVVQVDSATWRQALHMQREEWRQKINEVIGRERVREIVFR"
     gene            2025..3152
                     /locus_tag="AC16YU_01440"
     CDS             2025..3152
                     /locus_tag="AC16YU_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6063914.1"
                     /translation="MHPETLAARAGAEPDPETGAVSPPIHLSTTFERDPDGSYPKGYV
                     YAREDNPTRRRFEEALARLEGGAACAAFASGMAAASAVFASLAPGDLVVIPNDVYHGV
                     RRVLRSRFEAWGLRVSEADLSRPDSLHDALTDGTRLVWIETPSNPLLRITDIRAVSAR
                     AHEVGALCVVDGTWTTPLLQRPLELGADLVVHSVTKYIGGHSDVLGGAVVGLDADSDA
                     FAAVRTVQRSEGAVMDPFSAWLALRGLRSLGARLDRQCDTAARVAMTLAAHPAVEAVH
                     FPGLPTDPGHAVASRQMSRPGAMLSFRVKGGEAAALRVAAQVRLFTRATSLGGTESLI
                     EHRASVEGEATRTPRDLLRVSVGLEHPDDLLADLVSALEAP"
     gene            3250..4107
                     /locus_tag="AC16YU_01445"
     CDS             3250..4107
                     /locus_tag="AC16YU_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR6063915.1"
                     /translation="MKLPFLLARRFVAAETLEDTLPVVRRLHGQGLRVTLDHLGEHIS
                     DRRLAEQARDGYLDLVRTLGEAGGALDRNISIKLSMLGQQIDEAFCEDNLHLLLTSAR
                     DRDVFVRIDMEGSAVTASTLALFEKVFAVYPQHVGIVLQAYLKRTREDVERMCELNAR
                     VRLCKGAYREPASVAHQRMDAIREHFAEYMETLVTRGNYPGIATHDDILIERTKAFVA
                     ERGIGNDRFEFQMLYGIRPDTQVALASSGYNMRVYVPYGRMWVPYFSRRLRERKENIF
                     FVLRNLFRG"
     gene            complement(4112..5065)
                     /gene="trxB"
                     /locus_tag="AC16YU_01450"
     CDS             complement(4112..5065)
                     /gene="trxB"
                     /locus_tag="AC16YU_01450"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6063916.1"
                     /translation="MSTIDFAGAEHRQVVVVGTGPAGLTAALYAARANLSPLVLQGPE
                     PGGQLITTTDVENFPGFPEGILGPELMGKFEAQATRFGAELRWGTVTSVDLSGPPHRL
                     VVDEETPITADAVIISTGASARYLGLENEKRLLGHGVSACATCDGAFFRGQEVAIVGG
                     GDTAMEEALFLTRFASTVHLVHRREELRASQIMQERAMKSDKIRFVWNTVVEDVLGER
                     EVTGLRVRNVKTGEESVLPVQGFFVAIGHSPNTALFKGLLDMDETGYIRTRPGSTWTN
                     VEGVFACGDAQDHVYRQAVTAAGTGCMAAIDAERWLAERSA"
     gene            complement(5062..5412)
                     /gene="trxA"
                     /locus_tag="AC16YU_01455"
     CDS             complement(5062..5412)
                     /gene="trxA"
                     /locus_tag="AC16YU_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6063917.1"
                     /translation="MADDAKYVVVNDQNFVKEVLESEVPVLVDFWAAWCGPCRIIAPI
                     VKELAAEFEGRAKVAKLDVDNNPQVAHRFHIQSIPTLLFFKDGHVVDQLIGTAPKRAL
                     KERLESLAVQTAQS"
     gene            complement(5558..7828)
                     /gene="purL"
                     /locus_tag="AC16YU_01460"
     CDS             complement(5558..7828)
                     /gene="purL"
                     /locus_tag="AC16YU_01460"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR6063918.1"
                     /translation="MAHSAHSETTVTLELAREHGLTDEEYGWVLERLGRTPTFVELGI
                     YSVMWSEHCSYKNSIAVLKTLPREGEALLVGAGEENAGLVDIGDGLAVAFKIESHNHP
                     SAVEPYQGAATGVGGIQRDIFTMGARPICSLDSLRFGSLENPRVRYLFDGVVRGIGDY
                     GNSFGVPTVAGEVCFDPSYEGNPLVNAMSVGVVRIGETASAIAEGPGNPVYIVGSATG
                     RDGIHGATFASEEITEASESRRPSVQVGDPFTEKLLLEATLEVIHSGVVVGIQDMGAA
                     GLTCSSCEMSAKGSSGMDLFVDRVPMRETNMTPYEVMLSESQERMLIVCRKGREAELQ
                     AIFEKWDLHAVRIGEVTATDRVRVFWHGELVADVPAEHLVLGGGAPVYHRETRRPEYL
                     DRVAAFDASAVPDTIPESASEVLLRLLGSPNIASKRWVFDQYDTMVRTNTVAGPGPTD
                     AAVVRIKGTGKGLAVKTDCNGRYVYLNPRRGGQIAVAEAARNVVCAGGRPVAITNCLN
                     FGNPYKPEVYWVFREAVGGMGDACRALGTPVTGGNVSFYNENPAGAVFPTPTIGMLGV
                     VDDVEAHTTTSAFREAGDVIALLTPSGWVHRDDIAASEYLSVVHGRTDGDAPWMDLEE
                     ERSVQEACLALIRGGLVHSAHDVADGGLAVCLAEASIFSDGLGADVELAAKGTSRLDA
                     LLFGEAQSRILVTLGESDLDEALSVASSAGARLTRIGRVIRRSFRIGVDGVTVIHLTR
                     GQLAAPYETAIPSRMS"
     gene            complement(8025..8136)
                     /gene="rrf"
                     /locus_tag="AC16YU_01465"
     rRNA            complement(8025..8136)
                     /gene="rrf"
                     /locus_tag="AC16YU_01465"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(8229..>9070)
                     /locus_tag="AC16YU_01470"
     rRNA            complement(8229..>9070)
                     /locus_tag="AC16YU_01470"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1457 a         3226 c         2954 g         1433 t
ORIGIN      
        1 atcacgattc ccgacgagga tgccgagaag atcgccacgg taggcgatgc tgtttcgtac
       61 ctgtcggaga agtctgcctg aggcagccgc tccgcacggc ccgccccccg ggcctgacgc
      121 ccacccggaa accccgctgg aggaccccat ggatcgaccg atgcgtcgtg tcgtcgtgac
      181 gggcatggga gccgtcacgc ccatcggcct tacggtcgat gccttctggg acgccatgat
      241 ggcggggatc agcggcgccg ccccgatcgc ggcgttcgat gccgaggcct tcgacacgaa
      301 gttcgcgtgt gagctcaagg agttcgatcc cggggactac ctggaccgga agatcgcccg
      361 ccgactggac cgcttcagcc agctggcgct gatcgcggcc gaccaggccg tcgcggattc
      421 aggtatcgag cccgaccgcc tcacggagga cgagaaggac cgcgtcgcgg tcatcttcgg
      481 atccggcatt ggcggaatga aggtgttcca cgagcaggtc gtgacctatc acgagtcggg
      541 gccgaagcgg ctttcgccat tcttcatccc gatgctcatc ccggacatcg ccgccggcca
      601 gatctcgatc cgccacggct tccgggggcc gaactactgc gtggtctcgg cctgcgcgac
      661 gggcaacaac aacctgggcg acgccttcat gctcatccag gccgggcacg cggacgtggc
      721 ggtcagcggc ggggccgagg cgagcgtcac cgaaatggga gtgggcggct tcaacgcact
      781 gaaggccctc tcgacccgca acgacgaccc cacgcgcgcc agccgtccgt tcgatgcgac
      841 ccgcgacgga ttcgtgctcg gcgagggcgc cggcgccctg atcctcgagg agctcgggca
      901 cgcccgggcg cggggagccc ggatctacgc ggaggtggcc ggcatcggca tgagcgcgga
      961 cgcctaccac atcacggcgc ccgaccccga cggccggggc gcgcgcgccg ccatgaaggc
     1021 cgccctcctg cacgccggaa tcgcgccgac cgacgtggac tacctgaaca tgcacggcac
     1081 ctcgacgccg ctcggcgaca tcgccgagac caacgcgatc aaggccgtgt tcggggacca
     1141 cgcttaccgc atgaacgtct cctccacgaa aagcatgacc ggccacctcc tcggcgcggc
     1201 cggcgccgtg gaggcgatcg ccgccatcct cgcggtgcgg aacgaccgcg tcccgccgac
     1261 gatcaatttc gagcacgccg acgcggactg cgacctgaac tacacgttca acgcgcccga
     1321 ggaccggccg gtgcgcgtcg cgctcagcaa tgccttcggc tttggcggac acaacacgac
     1381 ggtcgcgttc cggaagttcg aagactgacc cgggaacgac gcccggggat gccccgccgg
     1441 aaggtcagcg gaagacgatc tcgcggacgc gctcacgccc gatcacctcg ttgatcttct
     1501 gccgccactc ctctcgctgc atgtgcaggg cctggcgcca cgtggccgag tcgacctgaa
     1561 ccaccaggcg gtcgccgtgc aggcgcacgc ggcgcgtgac ccgtccgatg cgcggcccgg
     1621 ccatcgcccg ccactcggcc tcgatccggg cctcttcgag ccggtccgtg aggccgaggc
     1681 cgtccaccac ttcggggatc agatcaccca ggctggcggg tgtcttgcgg cggctcatcg
     1741 ttccgtactg gctgttcgcg gggcacgata cgggtactcc ccccgctccg caacgatccg
     1801 gggccggatg aaacgggaaa gggagccccg ctggccgatt ccggcgcgcg aagcccccca
     1861 tcccttcatc ggaagacgca cgatccgata cgggagaccg gggccccggt tgcggcgcgc
     1921 acgcgatttg gcaccggacg atcctggccg tgccggtacg cgcccggtcg aacggtcggg
     1981 acgcctggcc taccttccgc gcgaacgcca ccccacgcta cgccatgcac ccggaaaccc
     2041 tggccgcacg ggccggagcg gaaccggacc ccgagaccgg ggccgtgtct ccgcccatcc
     2101 acctctcgac gaccttcgag cgcgacccgg acggctcgta cccgaagggc tacgtgtacg
     2161 cgcgcgagga caatccaacg cgccgccgat tcgaggaggc gctcgcccgc ctggagggcg
     2221 gcgcggcctg tgccgcgttc gcctccggca tggcggccgc gtcggccgtg ttcgcgtccc
     2281 tggcaccggg cgacctcgtg gtcattccga acgacgtcta ccacggggtt cggcgagtgc
     2341 tccggagccg gttcgaggca tgggggctgc gcgtttccga ggccgacctc tcgcggccgg
     2401 attccctcca cgatgccttg accgacggga cgcgcctggt ctggatcgag acgccgtcca
     2461 acccgctgct gcggatcacc gacatccggg ccgtgtccgc ccgcgcgcac gaggtcggtg
     2521 cgctctgcgt cgtggacgga acgtggacca cgccgctcct ccagcgaccg ctcgagctcg
     2581 gcgcggacct cgtcgtgcac tcggtgacga aatacatcgg cggccactcc gacgtcctgg
     2641 gcggagcggt cgtcggcctc gacgctgatt cggatgcgtt cgccgccgtc cgcacggtcc
     2701 agcgctccga gggagccgtc atggacccgt tctcggcctg gctggccctg cgcggactcc
     2761 gctcgctggg ggcccggctc gaccggcagt gcgacaccgc ggcacgggtc gctatgacgc
     2821 tcgccgcaca cccggccgtc gaggccgtgc acttccccgg ccttccgacg gacccgggcc
     2881 acgccgtcgc ctcccgccag atgagccggc ccggcgccat gctctcgttc cgcgtgaagg
     2941 gaggcgaggc cgcggccctg cgcgtcgccg cccaggtgcg gctcttcacg cgcgcgacca
     3001 gcctcggcgg caccgagagc ctgatcgaac accgcgcgtc ggtcgaaggc gaggccacgc
     3061 gcacgccgcg ggatctcctg cgcgtgtcgg tggggctgga acacccggac gacctcctcg
     3121 cggacctcgt ttcggccctc gaagccccgt aaaccccgct tgcgcccgca tggacgggtt
     3181 ggtagcttcc ggccgcctcg ccggcggcgt ccggcgggcg cggatcaaac cgggctcccc
     3241 gcccccgtca tgaagcttcc cttccttctc gcccggcgct tcgtcgcggc cgagaccctc
     3301 gaggataccc ttcccgtggt ccgccggctg cacggacagg ggctgcgcgt cacgctggac
     3361 cacctcggcg agcacatcag cgaccggcgc ctggccgagc aggcccggga cggctacctg
     3421 gacctggtcc ggaccctcgg cgaggccggg ggggcgctgg accgcaacat ctcgatcaag
     3481 ctttccatgc tcgggcagca gatcgacgag gcgttctgcg aggacaacct gcacctcctg
     3541 ctcacgtccg cccgtgaccg ggacgtcttc gtgcggatcg acatggaggg cagcgccgtc
     3601 accgcctcga cgctcgcgct cttcgagaag gtgttcgccg tgtaccccca gcacgtgggc
     3661 atcgtcctgc aggcctacct gaagcggacg cgcgaggacg tggagcggat gtgcgagctg
     3721 aatgcccgcg tgcggctgtg caagggcgcc taccgggaac cggcctcggt cgcgcaccag
     3781 cggatggacg cgatccgcga gcatttcgcc gagtacatgg agaccctcgt cacgcggggc
     3841 aactatcccg gaatcgccac ccacgacgac atcctcatag agcgcacgaa ggccttcgtc
     3901 gccgagcggg gcatcggcaa cgaccgcttc gaattccaga tgctgtacgg catccggccc
     3961 gacacgcagg tcgcgctcgc gtcgtccggt tacaacatgc gggtttacgt gccgtacggc
     4021 cgcatgtggg tgccctactt ctcgcggcga ctgcgcgaac gcaaggagaa catcttcttc
     4081 gtcctgcgca acctgttccg cggctgagcg ctcaggcgga tcgctccgcg agccagcgct
     4141 ccgcgtcgat cgctgccatg caccccgtcc cggctgccgt caccgcctgc cggtacacgt
     4201 ggtcctgcgc gtcgccgcag gcaaacacgc cctcgacgtt cgtccaggtg gatccgggcc
     4261 gggtccggat gtagcccgtc tcgtccatgt ccaggaggcc cttgaaaagc gcggtgttgg
     4321 gcgaatgacc gatcgcgacg aagaatccct gcaccggcag gaccgattcc tctccggtct
     4381 tcacgttgcg cacccgcagc cccgtcactt cccgctcccc gagcacatcc tcgaccaccg
     4441 tgttccagac gaagcggatc ttgtccgact tcatggcgcg ctcctgcatg atctgcgagg
     4501 cacgcagctc ctcccggcgg tggacgaggt gaacggtcga ggcgaagcgc gtcaggaaca
     4561 gtgcctcctc catggccgtg tcgccccctc cgacgatggc cacctcctgc ccgcggaaga
     4621 aggccccgtc gcaggtggcg caggccgaca ccccgtggcc caacaggcgc ttctcgttct
     4681 cgaggcccag gtagcgcgcg gacgcacccg tcgagatgat gacggcgtcc gccgtgatcg
     4741 gcgtctcctc gtccaccacg aggcggtgcg gaggaccgga caggtcgacc gacgtgaccg
     4801 tcccccagcg cagctccgcg ccgaagcgcg tggcctgcgc ctcgaacttg cccatgagct
     4861 ccggcccgag gatgccctcc ggaaagcccg ggaagttctc gacgtcggtg gtcgtgatga
     4921 gctgccctcc gggctccggc ccctggagca ccaggggcga gagattcgcg cgcgccgcgt
     4981 acagggcagc cgtcaggccg gccggtcccg ttccgacgac gaccacctgg cggtgctcgg
     5041 cgccggcgaa atcgatggtg ctcatgactg cgcggtctgc acggccagcg actccagacg
     5101 ttccttcagg gcgcgcttcg gcgccgttcc gatcagttgg tccaccacgt ggccgtcctt
     5161 gaagaaaagg agcgtcggga tcgattggat atggaatctg tgcgcgacct gggggttgtt
     5221 gtccacgtcg agcttggcca ccttcgcgcg gccctcgaac tcggcggcga gctccttgac
     5281 gatgggggcg atgatgcggc agggaccaca ccacgccgcc cagaagtcca cgagcacggg
     5341 tacctcggac tcgagcacct ccttgacgaa attctggtca ttcaccacga cgtacttggc
     5401 atcgtctgcc atcattcacg ctccgcggca tccgcgatgc cggctggttg attttcgaag
     5461 ttgaggatga ttgagtgtct ttcgaggcac agccgttacg cgggccggtc cgcggggctc
     5521 cgcaaggccc gtgccggcgg gccgtcccgc cagagagtca ggacattcgc gacggaatgg
     5581 cggtttcata gggtgcggca agctgtcccc gtgtcaggtg gatgacggtg actccgtcca
     5641 cgccgatccg gaacgagcgt cggatcacgc gcccgatgcg agtcagcctg gccccggccg
     5701 acgacgcgac ggagagcgcc tcgtcgaggt cggactcgcc gagcgtcacc aggatgcggg
     5761 actgggcctc gccgaacagc agggcgtcga ggcgcgacgt gcccttggcc gcgagctcca
     5821 cgtcggcccc caggccgtcg gaaaagatcg aggcctccgc caggcacacg gcgagcccgc
     5881 cgtcggccac gtcgtgggcg ctgtgcacga ggcccccgcg gatcagcgcg aggcaggctt
     5941 cctggaccga gcgctcctcc tccaggtcca tccagggagc gtcgccgtcc gtccggccgt
     6001 gcacgacgga caggtattcc gacgccgcga tgtcgtcgcg gtgcacccag cccgaggggg
     6061 tcaggagcgc aatcacgtcg cccgcttccc ggaatgccga ggtcgtcgtg tgggcctcga
     6121 cgtcgtcgac gacgcccagc atgccgatgg tgggagtcgg gaagacggcg cccgcgggat
     6181 tctcgttgta gaacgacacg tttcccccgg tcaccggcgt gcccagggcc cggcacgcgt
     6241 cccccatgcc cccgaccgcc tccctgaaca cccagtagac ctcgggcttg tacggattgc
     6301 cgaagttcag gcagttggtg atggcaaccg gccggccccc ggcgcagacg acgttgcgcg
     6361 cggcctcggc gaccgcgatc tgcccgcccc gtcgcggatt caggtacacg tagcgcccgt
     6421 tgcagtcggt cttgacggcg agacccttcc cggtcccctt gatccggacg acggccgcat
     6481 ccgtggggcc gggaccggcg acggtgttcg tgcgcaccat ggtgtcgtac tggtcgaaca
     6541 cccagcgctt ggacgcgatg ttcggcgagc cgagaaggcg cagcagcacc tcggacgccg
     6601 actcggggat cgtgtccggc accgccgacg cgtcgaaggc cgcgacccgg tccaggtact
     6661 ccggccggcg cgtttcccgg tggtagaccg gggcgccccc accgagcacg agatgctcgg
     6721 ccggcacgtc ggcgacgagc tcgccgtgcc agaacacccg cacccggtcg gtcgccgtga
     6781 cctcgccgat ccgtacggcg tgcaggtccc acttctcgaa gatcgcctgg agctccgcct
     6841 cgcggccctt ccggcacacg atgagcatgc gctcctggct ctccgagagc atgacctcgt
     6901 acggcgtcat gttcgtctcg cgcatcggca cgcgatcgac gaacaggtcc attccgctcg
     6961 agcccttcgc gctcatctcg cacgacgagc acgtgagccc cgctgccccc atgtcctgga
     7021 tgccgacgac gacccccgag tggatcactt cgagcgtcgc ctccagcagg agtttctcgg
     7081 tgaacgggtc gcccacctgg acggacgggc gccgcgattc ggaggcctcc gtgatttcct
     7141 ccgacgcaaa cgtcgcaccg tggatgccgt ccctgccggt ggccgagccc acgatgtaca
     7201 cggggttgcc cggcccctcg gcgatcgccg aggccgtctc cccgatgcgg acgacgccga
     7261 cgctcatcgc gttgacgaga gggttgccct cgtacgaggg atcgaagcac acctcgcccg
     7321 cgaccgtcgg cacgccgaac gagttgccgt agtcgccgat gccgcggacg accccgtcga
     7381 acaggtagcg gacgcgggga ttctcgagcg atccgaagcg gagcgaatcg agtgagcaga
     7441 tgggacgcgc gcccatcgtg aagatgtcac gctggatgcc cccgacgccg gtagccgcgc
     7501 cctggtaggg ctccaccgcc gacgggtggt tgtgcgattc gatcttgaag gcgacggcaa
     7561 gcccgtcccc gatgtccacc agccccgcgt tctcctcgcc ggctcccacg aggagcgcct
     7621 ccccctcgcg ggggagcgtc ttgagaacgg cgatggaatt cttgtacgag cagtgctcgc
     7681 tccacatcac cgagtagatg cccagctcca cgaacgtggg cgtgcgcccg agccgctcga
     7741 gcacccagcc gtactcctcg tcggtcaggc cgtgttcgcg cgcgagttcg agggtgaccg
     7801 tcgtttcgga gtgcgcgctg tgggccatgt cgctgcgttt cggtgacggc ggcgggagcg
     7861 ccggggtgcc gggagcggtg agccgcaaag atacgatcgg ggctcggccg ccgggcggga
     7921 atatcccgtt caggaaggcc accggcccgc gaggtgcctg cagaagaagc gcccataaac
     7981 gaaaagcgcc ccccgatcag acgatcggag ggcgctcatg aagatctcgg cggcgaccta
     8041 ctctcccacc acttgggcag taccatcggc gctgcggggc ttaactgctc tgttcggaat
     8101 gggaagaggt ggaccccccg cgcgatagcc accgagaagc cggtgacacg atgactgttc
     8161 cgtagcgcaa ggcgcaagga tgagtaccgc acgatcgcct ggcgtgaggc ggtgcatgta
     8221 caaagacagg aaataagcct gacgggtaat tagtacggct cggctgaaca cattgctgtg
     8281 cgtacacctg ccgcctatca acgtcctagt ctggaacgac ccgtagggga aatctagtct
     8341 tgcggtgggc ttcgtgctta gatgctttca gcgcttatcc catccgaaca tagctaccct
     8401 gcgatgcagc tggcgccaca acaggtacac tagaggttcg tccacttcgg tcctctcgta
     8461 ctagaagcag atccgcgcaa atttccaacg cccacagcag atagggaccg aactgtctca
     8521 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
     8581 cttctccagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
     8641 gaactcttgg gggagatcag cctgttatcc ccggcgtacc ttttatcctt tgagcgacgg
     8701 cccttccatt cggaaccgcc ggatcactaa gtcctgcttt cgcacctgct cgacctgtat
     8761 gtctcgcagt taagcaccct tgtgccttta cactctacgc acgattaccg accgtgctga
     8821 gggtaccttt gagagcctcc gttactcttt gggaggcgac cgccccagtc aaactaccca
     8881 cctgacactg ttcctcgacc ggataacggc cgaaggttag gcttcaacca atgggagggc
     8941 ggtatttcaa ggttgactcc accagagctg gcgccctggc ttcacagtct cccgcctatc
     9001 ctacacacct attggacgaa gtcaatgcca agctgcagta aaggtgcacg gggtctttcc
     9061 gtcccgctgc
//
LOCUS       DBNHQZ010000061         3089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2048343, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000061 DBNHQZ010000000
VERSION     DBNHQZ010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2048343"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="AC16YU_01475"
     CDS             complement(<1..791)
                     /locus_tag="AC16YU_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6063919.1"
                     /translation="MASLTETSIRRPVATAMAYLVVITIGVVGFMFLPVDLLPPVEFA
                     RLTVFTSYPNVGPEEMEQIVTDPIENALSGIPNLDRITSSSSEGSSRVTLEFARGTSL
                     DEAANDVRSSLDRVRRNLPPEVDPPVIWKFDPNNFSIVTLAVESRRPLEEVTRLLERD
                     ISNRFEQIPGVGTVELRGGINRQIHVDLERDRLQAASLTPADVQAAIARENQKLPGGN
                     VKEGVRDLYVRSLGEYTTVEQIRQTVIRYVDDRPVRVQDVANVVDG"
     gene            complement(904..2244)
                     /locus_tag="AC16YU_01480"
     CDS             complement(904..2244)
                     /locus_tag="AC16YU_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6063920.1"
                     /translation="MSRYIAGLVPLVVLALTAGCGTDTGGPASGAGRPGAPIPSVEVV
                     QAKMGSLPLRERLSGMVRAQNQVSVVSEISAPVERVVARNGDRVSAGDALAYLRDKQY
                     QDQLRQAEAALQIAHADQRRTEATLAELRTRLERTRQLAEKEYESAAGLELLQAQAAG
                     AEAANEQALARIAQAEATVEEQREALRRTVVRAPVSGVVGERNVEVGMRIDPGRPLFM
                     VGDLSRIRVEVAVTDRMVGRIEAGQTALITVEGSTEPPIEAQVSRISPFLQEGTFTAA
                     AEIDLVNPDGRLRPGMFVAVDVLYGESEQATVVPEAALFEDPNSGILGAFVATSLRTE
                     TPVEEPEEYDPDNPPPLVGPTPVSFQPLDVLARGDGLAGVSGIQAGDWVLTVGQHLIR
                     SLDGTGQVLARPVPWTRVASLQRLQDRDLLLRFLEKQQRLARESFTDNGSAASK"
     gene            complement(2414..2686)
                     /locus_tag="AC16YU_01485"
     CDS             complement(2414..2686)
                     /locus_tag="AC16YU_01485"
                     /inference="COORDINATES: protein motif:HMM:NF021873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR6063921.1"
                     /translation="MQPKYPGVVAVRPLERKQLDIHFRDGSRKAYDGSPLLVEDAFAP
                     LAVESLFRQVRVEPHGYAVSWDDDIDLAGSELWLNGKTVAEGAQVD"
     gene            complement(2764..>3089)
                     /locus_tag="AC16YU_01490"
     CDS             complement(2764..>3089)
                     /locus_tag="AC16YU_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063922.1"
                     /translation="TFNRMLSVLLGDAPEGRERQYVRAGWALSVMYYLGGAFATTLLP
                     VPELGLTAAARGTLDLPGGGLWVDEPQRVLAFGFLYFGMTGIGEVLGWARNEKFLKGV
                     PKDPE"
BASE COUNT          435 a         1082 c         1100 g          472 t
ORIGIN      
        1 ccgtccacga cgttcgcgac gtcctgcacc cggaccggcc ggtcgtccac gtagcggatc
       61 accgtctgcc ggatctgctc gacggtcgtg tattcaccca gcgaccggac gtacaggtcg
      121 cgtaccccct ccttcacgtt ccctcccggg agcttctggt tctccctcgc gatggcggcc
      181 tgcacgtcgg ccggcgtaag gctcgcggcc tgcagccggt cccgctccag gtccacgtgg
      241 atctgccggt tgatgccccc gcgaagctcc accgtaccga cccccgggat ctgctcgaac
      301 cggttcgaga tgtcgcgctc caggagccgc gtcacctcct cgagggggcg gcgcgactcg
      361 accgcgagcg tcacgatcga gaagttgttc gggtcgaact tccagatgac gggcgggtcc
      421 acctcgggcg ggaggttccg ccgcacccgg tccagcgagg accggacgtc gttggccgcc
      481 tcgtccagac tggtgccgcg cgcgaactcg agggtcaccc ggctcgatcc ctcggacgag
      541 ctggacgtga tgcggtccag gttggggatg cccgagagcg cattctcgat cgggtccgtg
      601 acgatctgct ccatctcttc cgggcccacg tttggatagc tcgtgaacac ggtgaggcgg
      661 gcgaactcca cgggcggcag gaggtcgacc ggcaggaaca tgaatccgac cacgccgatc
      721 gtgatgacga ccaggtaggc catcgcggtc gcgacgggtc gacggatgga ggtctcggtc
      781 agtgatgcca tggctcggcc gttggtcgct gggggtgcgc ggggatcggg cggaagcggg
      841 gggtgcggac ggacgctgtg ggtgcgcggg ttcgggcgga tgcggtccgg ggtcagtccg
      901 cggtcatttc gaggcggcgg acccgttgtc cgtgaacgat tcgcgggcca ggcgctgctg
      961 cttctcgagg aaacgcagca gcaggtcgcg gtcctgcagg cgctgcagcg aggccacgcg
     1021 cgtccacggg acgggccgcg ccaggacctg gcccgtgccg tcgagcgagc ggatcaggtg
     1081 ctggccgacg gtcagcaccc agtcgccggc ctggatgccc gagacgccgg cgaggccgtc
     1141 gccgcgggcc agcacgtcga gcggctggaa cgagacgggg gtcgggccca cgagcggggg
     1201 cggattgtcc gggtcgtact cctcgggctc ctcgacgggc gtctcggtgc gcagcgaagt
     1261 ggcgacgaag gccccgagga tgccgctgtt cggatcctcg aagagcgcgg cctcgggaac
     1321 gacggtggcc tgctcggact cgccgtagag cacgtccacg gcgacgaaca tgcccggccg
     1381 aagccgcccg tccggattca cgaggtcgat ctcggcggcg gcggtgaagg taccctcctg
     1441 caggaagggc gaaatccggg acacctgggc ctcgatcggc ggctcggtgg atccctccac
     1501 ggtgatgagc gccgtctgcc cggcctcgat gcggcccacc atgcggtcgg tcaccgccac
     1561 ctcgacgcgg atcctggaca ggtcgcccac catgaagagc ggacgacccg gatcgatgcg
     1621 catgcccacc tcgacgttgc gctcgcccac gacccccgaa accggggccc ggaccacggt
     1681 ccggcggagg gcctcgcgct gctcctcgac ggtcgcctcg gcctgcgcga tgcgggcgag
     1741 ggcctgctcg ttcgcggcct cggcgcctgc ggcctgcgcc tggagcagct ccaggcccgc
     1801 cgcgctctcg tattccttct cggccagctg ccgggtgcgc tcgagccggg tccggagctc
     1861 ggcgagcgtc gcctctgtcc ggcgctggtc cgcgtgcgcg atctgcagcg cggcctcggc
     1921 ctgccggagc tggtcctggt actgcttgtc cctcaggtac gcgagggcgt cgcccgcgct
     1981 cacccggtcg ccgttgcgcg ccacgacgcg ctcgaccggc gccgagatct cggacacgac
     2041 ggacacctgg ttctgcgcac ggaccatgcc gctgagccgc tcgcggagcg gcaggctgcc
     2101 catcttggcc tggacgacct cgacggacgg aatcggcgcc ccgggccgac ccgcgcccga
     2161 ggccggtccc ccggtatcgg taccgcagcc ggccgtgagt gccaggacga cgagcgggac
     2221 gaggcccgcg atatatctgg acatgggtgg attgctttcc cgttcaggcc ttgaagaacg
     2281 gcccagcaat cgggcagttc acccccgtct gtcacggaag tgtgtcgatt ccgcggacgg
     2341 agggtggaga cggggggtgg gtgcgtggcg gcggtggggg cgccgcgggt ggttcggcgg
     2401 caggccctct ccgtcagtcg acctgtgccc cttccgcgac ggttttcccg ttcagccaca
     2461 actccgagcc cgcgaggtcg atgtcgtcgt cccagctgac ggcatacccg tgaggctcga
     2521 cccggacctg gcggaacagc gactcgacgg cgagcggcgc gaaagcgtcc tccacgagga
     2581 gcggcgagcc gtcgtatgcc ttgcgcgacc catcgcggaa gtggatgtcc aattgcttcc
     2641 gctccagcgg ccgcacggcc acgaccccgg gatatttcgg ttgcatcgtc attcgtccag
     2701 acccggatgc tccctgaaat cctgcgtcac ccacatggcc cggaggtccg gccggcgagg
     2761 ttcctattcc gggtctttgg gcacgccctt caggaacttc tcgttccgcg cccaccccag
     2821 cacctcgccg attcccgtca tcccgaagta caggaagccg aaagccagga cgcgctgggg
     2881 ctcgtccacc cagagcccgc cgcccgggag gtcgagcgtg ccgcgcgcgg ccgccgtgag
     2941 ccccagctcg ggcacgggca ggagcgtggt cgcgaaggcg ccgcccaggt agtacatcac
     3001 cgagagcgcc caccccgccc ggacgtactg tcgctcccgg ccctcgggcg cgtcgcccag
     3061 caggacggag agcatgcggt tgaaggtca
//
LOCUS       DBNHQZ010000062         3895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3267543, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000062 DBNHQZ010000000
VERSION     DBNHQZ010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3267543"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            569..2215
                     /locus_tag="AC16YU_01495"
     CDS             569..2215
                     /locus_tag="AC16YU_01495"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR6063923.1"
                     /translation="MSDDPRATAGGLLIQSIDVLADAPEKAPSIFNDVLGPVMRGPSS
                     SHTAASWRIGRLATQMLDEPLAEALVEFDEMGAWAPNYEEQGTVLGMTGGLLGIEITD
                     ARIVHGTRIGQDSGIRIEYRVSRFPTDHANTVRLALTGRHGRRLVLVAVSLGGGMIEV
                     RMIDGHPVRLQGDWFELLGWVGSDGSGPVRSAVEERMGEDHALTWSETGSGCLFRVRS
                     PQPFDPEAVERLAGLHGVSRVALVRPILPVVSGREGAFPFRTYADLVARGADAGDSLG
                     ELGLRYEAARSGLERAEVVRMMQDLVGIIEASIDTGLAGTEYADRILPRQSDRIRAAE
                     SRGILPADGLVNRLIENVTAIMEAKSAMEVIVAAPTAGGCGTLGGTIRAVADARGAPG
                     DAKIRAYFAAGLVGVFIAQGPGFSAEEGGCQLETGAAASMAAAALVDLCGGTTAQALA
                     GASMALQNTIGLVCDPVADRVEVPCLGKNVTAGMNALAASTMALAGFDGVIPLDEVIV
                     TVKSVAATMPRALCCTGLGGLAATPTSARLKEELRIRCSC"
     gene            complement(2244..3731)
                     /locus_tag="AC16YU_01500"
     CDS             complement(2244..3731)
                     /locus_tag="AC16YU_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6063924.1"
                     /translation="MTTGADDSTATPRVLLTGATGYVGGRLLTALGNRDFRVRCLARR
                     PAVLESRAGPSTEVVEGDVLDRPSLDAALRGVDAAYYLVHSMGSDGSFEQMDRQAARN
                     FGEAAKAAGVSRIVYLGGLGNTDEALSAHLRSRHEVGDVLRESGVPVIEFRASIIIGS
                     GSLSYEMIRSLVERLPIMITPRWVQVPAQPIAIDDVLEYLVAALQLPLSGSRVYEIGG
                     ADVVSYADIMRAYARQRGIRLRMIPVPVLTPYVSSLWLGLVTPLYARIGRKLIESIVH
                     STVVRDPAALEAFPIRPIGIEEAIRSAIRSEEREAAETRWSDAVSSSGELPSWDGMQF
                     GSRLVDSRTVSVAAAPDAAFAPILRIGGRTGWYAWNWLWRLRGYLDMLVGGIGMRRGR
                     RSETSLSVGDTVDFWRVEAVDANRLLLVAEMKLPGRAWLEFEVASDGSSATIRQTAIF
                     DPVGLPGRAYWYALYPLHHLVFGGMLRGIARAALRESTTHGPVMP"
BASE COUNT          591 a         1327 c         1367 g          610 t
ORIGIN      
        1 cctgagcctg ttcggtgcag gtgacggaga agctgacggt gcccagggga tggccggcgt
       61 gctgagaaac ctcgcgtttc gtttcgtccg cacagcccat gagtgaaaca accatgaggc
      121 cggtagcgag gcacaggaag ccaagacgac gtgcgcgcgc caccgaccca cgtggtgtcc
      181 gtccaggaag tctgccgatt gtcattgctt gctccgtggg ccgcttcgcc gctcatcgtg
      241 aatccgcgag ggtttccgtg ccacccaacc tgtcttctac gggcacgccg cgcccatcca
      301 aaagaggtgg tcgacggttg cggttcgaga aagtgtaatg gctgcaagga gttgcgtcaa
      361 gcggtacgca agaggcgtat tgaaaggccg catatcgccg gaccgcatat cgccggacca
      421 catatcaccg ctccgggatc cccccgcatc cggtacccgc aggcgattcc gttggcccgg
      481 cacgtttcac ggccgaatcc ccccgcccgc ccctcgactc tcggtcgcgg ttgccctatg
      541 atccgatacc gaaacacccc ctttcgcgat gtccgacgac cctcgtgcca cggcaggcgg
      601 actcttgatc cagtcgatcg acgttctggc cgatgcgcct gaaaaggctc cgagcatctt
      661 caacgacgtc ctgggccccg tcatgcgcgg gccgtccagc tcgcacaccg ccgcgtcctg
      721 gcggatcggc cggctggcga cccagatgct ggacgagccg cttgccgaag ccctggtcga
      781 gttcgacgaa atgggcgcgt gggcccctaa ctacgaggag cagggcacgg tgctcgggat
      841 gaccggcgga ctgctgggca tcgagatcac cgacgcgcgc atcgtccatg gcacccgcat
      901 cgggcaggac tcggggatcc ggatcgagta ccgggtgtcg cgattcccga ccgatcatgc
      961 gaacacggtc cggctcgccc tgacgggtcg gcacgggcgg cgcctggtgc tggtcgcggt
     1021 gtcgctcggc gggggcatga tcgaggtccg gatgatcgac ggccacccgg ttcggctgca
     1081 gggcgactgg ttcgagctgc tcgggtgggt cgggtcggac ggatccggtc cggtcaggtc
     1141 cgccgtggag gaacgcatgg gtgaggatca tgcgctcacc tggtccgaaa ccggatcggg
     1201 ctgcctgttc cgcgtccggt ccccgcagcc gttcgatccg gaggccgtcg agcgcctcgc
     1261 gggactgcac ggcgtaagcc gcgtcgcgct cgttcggccc attcttccgg tcgtctccgg
     1321 tcgggaggga gcgttcccgt tccggacgta cgcggacctg gtcgcccggg gggcggacgc
     1381 gggcgattcg ctgggtgagc tcgggctgcg gtacgaggcc gctcggagcg gcctggagag
     1441 ggccgaggtg gtccggatga tgcaggacct cgtcgggatc atcgaggcga gcatcgatac
     1501 gggcctggcg gggaccgagt acgcggaccg gatcctgccg cggcagtccg atcgtatccg
     1561 cgccgcggag tcgcggggca tcctgccggc ggacggcctc gtcaatcggc tcatcgagaa
     1621 cgtgacggcc atcatggagg ccaagagcgc gatggaggtg atcgtggcgg cgccgaccgc
     1681 gggcggctgc ggtacgctgg gggggacgat cagggcggtg gccgacgcgc ggggcgcgcc
     1741 cggcgacgcg aagatccggg cctacttcgc cgccgggttg gtgggcgtct tcatcgcgca
     1801 ggggccgggc ttctccgcgg aggagggggg atgccagctc gagaccgggg ccgccgcgtc
     1861 gatggcggcc gcggcactgg tcgatctctg cgggggcacc acggcccagg cactcgcggg
     1921 ggcgtcgatg gcgctccaga acacgatcgg tctggtgtgc gacccggtgg cggaccgcgt
     1981 cgaggtgccc tgcctgggga agaacgtcac ggccggcatg aacgccctgg ccgcgtccac
     2041 catggcgctc gcgggcttcg acggcgtaat tccgctcgac gaggtgatcg ttaccgtcaa
     2101 gagtgtcgcc gcgaccatgc ccagggccct gtgctgcacg gggctgggag ggctggcggc
     2161 cacgccgacg tcggcccggc tcaaggagga actgcggatc cgctgttcct gctagacgaa
     2221 gcccggccgc gaatccgctt cgttcagggc attaccggcc cgtgcgtcgt gctctcgcgc
     2281 agggcggcac gcgcgatgcc ccgcagcatg ccgccgaaca ccaggtggtg cagcgggtag
     2341 agcgcatacc agtaggctct ccccggcagc ccgaccggat cgaagatcgc ggtctgccgg
     2401 atggtcgcgg acgagccgtc gctggccact tcgaactcca gccaggcccg tcccggcagc
     2461 ttcatctcgg cgacgagtag cagccggttc gcgtcgaccg cctccacccg ccagaaatcg
     2521 accgtgtcgc cgacgctgag gctcgtttcg gaacgccgcc cgcgtctcat gccgatgccc
     2581 ccgacgagca tgtccaggta gcccctcagt cgccagagcc agttccacgc ataccagccc
     2641 gtcctgccgc caatgcgaag tatgggagcg aaggcggcgt ccggcgccgc ggcgacgctc
     2701 acggtccgcg agtccaccag tctcgaaccg aactgcatgc cgtcccacga gggcagctcg
     2761 cccgacgagg acacggcgtc ggaccagcgg gtttccgcgg cctctcgttc ctcactccgg
     2821 atggcgctgc gaatggcctc ttcgatgccg atcgggcgga tggggaatgc ctcgagcgcg
     2881 gcagggtccc gtaccaccgt gctgtggacg atgctctcga tgagcttgcg gccgatgcgc
     2941 gcatagagag gcgtgacgag gccgagccag aggctggaca cgtacggggt gaggaccggc
     3001 acggggatca tgcgcagacg gatcccacgc tgccgggcat acgcgcgcat gatgtcggcg
     3061 taggacacca cgtcggcgcc gccgatttcg tagacccggc tcccggatag cggaagctga
     3121 agcgccgcga ccaggtactc cagcacgtcg tcgatggcga tgggctgcgc cggtacctgg
     3181 acccacctgg gcgtgatcat gatcgggagc cgttccacga gcgagcgaat catctcgtac
     3241 gaaaggctgc ccgatcctat gatgatcgac gcgcggaatt cgatcaccgg cacgcccgat
     3301 tcgcgcagca cgtctccgac ctcgtgtcgg cttcggagat gcgcggagag ggcctcgtcc
     3361 gtgttgccca ggccgcccag gtacacgatg cggctcaccc ctgcggcctt tgccgcctcg
     3421 ccgaagttcc gtgccgcctg ccggtccatc tgttcgaacg agccgtccga acccatcgag
     3481 tgtacgaggt agtaagccgc gtcgacgccg cgcagcgcgg catcgaggct cggccgatcg
     3541 agaacgtcgc cctcgacgac ttccgtcgac ggaccggccc tcgactcgag cacggccggg
     3601 cgcctggcaa ggcagcgcac acggaagtcc cggttcccga gcgccgtgag gagccgcccg
     3661 ccgacgtacc cggtcgcccc ggtgagcagc acccgcggcg ttgccgtgga gtcatccgcg
     3721 cctgtcgtca tctcaccttc cgccggccat ggcccgccgt cctgaggcct ggcctgaaat
     3781 ccgtggcccg cgccctgtcc gttcgcggtc attcggtgac cgccggcgcc tgggcggagt
     3841 ccggggccgc cggctgtggc gccgaatccg gctgcggctc cgcttccgcc tccgc
//
LOCUS       DBNHQZ010000063         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_151864, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000063 DBNHQZ010000000
VERSION     DBNHQZ010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_151864"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="AC16YU_01505"
     CDS             <1..879
                     /locus_tag="AC16YU_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR6063925.1"
                     /translation="VAGAVRLELEQFLNRHPTTGEAIASRVIQAARARHASRAAVQSV
                     RRATSVSHRLNLPGKLADCSSTDPSDSEIFIVEGDSAGGSAKQGRERRFQAILPLRGK
                     VLNAEQATLAKVSENRELSNVVQALGCGLGDGLRLEHLRYHKIILLMDADSDGHHIAT
                     LLLTFFYRYMRPLIDGGYVYLAQPPLYRIDALSGTWWALDDADRAQIEGEIRKKNRQA
                     RIEVQRFKGLGEMMPATLWETTLDPDRRRLLRVSIPDDERLRTEQVISDLMGKEASAR
                     FDFIMNHASEVEELDV"
     gene            1010..1085
                     /locus_tag="AC16YU_01510"
     tRNA            1010..1085
                     /locus_tag="AC16YU_01510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Lys,seq:ctt)
     gene            complement(1152..2369)
                     /locus_tag="AC16YU_01515"
     CDS             complement(1152..2369)
                     /locus_tag="AC16YU_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6063926.1"
                     /translation="MIRSILTGLSALAINPMRSALSTLGVIIGVAALVAILALGDGLE
                     RFSREQVEATTDLHVVTVAVQSDVRTDGIVVRRDSLPHFSRAHLDALLDRIDVPVTGY
                     RMLVGSAMARPPDSTRDVGILIRAVDTSATLGERVRVLHGDLEAWRARPDRVTAVLSR
                     AAAEAFFPSGTERAPGGRVTIGGLRIPVAAVLDGRPASRPLEIYVPLNDSTEAWLFQA
                     GRVPSILLEARQVEDVDGIARAAGAWAAERYPGDLVRVQSNRARVEQVSRGLLVFKLG
                     LASIAAISLLVGGIGIMNILLASVSERTREIGIRKSAGARARDIQMQFLAESVAISGL
                     GSLVGVVVGMGGALLATGIIRSITDAPVHAAFTWQSIAVACAAAIVVGLVFGTYPARK
                     AARLSPIDAIRHE"
     gene            2486..>2787
                     /gene="tsaA"
                     /locus_tag="AC16YU_01520"
     CDS             2486..>2787
                     /gene="tsaA"
                     /locus_tag="AC16YU_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR6063927.1"
                     /translation="MIPFQAVGHVRSPYSEAVQVPRGPGAEHRAEGVIEVLPEFEAGL
                     KDIEGFSHLFVIWVFDRAEGYDLMSTPPTDDRSHGVFATRSPRRPNPIGLTVVELLG"
BASE COUNT          447 a          951 c          985 g          404 t
ORIGIN      
        1 gtcgcgggcg ccgtccggct cgagctcgag cagttcctga accgtcatcc gacgaccggc
       61 gaggcgatcg cgtcgcgggt catccaggcc gcgcgggccc ggcatgcgag ccgggcggcg
      121 gtgcagagcg tgaggcgcgc aacgagcgtt tcgcaccgcc tgaacctgcc ggggaagctc
      181 gccgactgct cgtccaccga tccgtcggac agcgagatct tcatcgtcga gggcgactcg
      241 gcgggcggct ccgccaagca gggacgggag cgcaggttcc aggcgattct cccgctgcgc
      301 ggcaaggtgc tgaacgcgga gcaggccacg ctcgccaagg tgtccgaaaa ccgcgagctc
      361 tcgaacgtcg ttcaggccct cggatgcggt ctcggagacg gcctcaggct ggagcacctc
      421 aggtatcaca agatcatcct gctgatggat gccgactcgg acgggcatca catcgccacc
      481 ctgctcctca cgtttttcta ccggtacatg cgcccgctca tcgacggggg atacgtgtac
      541 ctggcccagc cgccgctcta tcgcatcgac gccttgagcg ggacgtggtg ggcgctcgac
      601 gacgccgatc gcgcgcagat cgaaggcgag atccggaaga agaaccgcca ggcgcggatc
      661 gaggtgcagc gtttcaaggg actgggtgag atgatgccgg caaccctctg ggagacaacg
      721 ctggaccccg atcgccgccg ccttctccgg gtcagcatcc cggacgacga gcggctcagg
      781 acggagcagg tcatctcgga cctcatgggg aaggaggcgt ccgcgcggtt cgacttcatc
      841 atgaaccacg cgtccgaggt cgaagagctg gacgtgtgag tgctgtggcg tcttgcgact
      901 ccgcgctgca tccggccggc ctggattgcg gagacgcgcg tgaaaaggcg gcatacctcc
      961 ggcgcgctgg agaacccgcc ggtcagagac tcgtatattc gggctctgca ggcccgtagc
     1021 tcaactggca gagcagctga ctcttaatca gcgggttcgg ggttcgagtc cccgcgggcc
     1081 taccacttcg agggcgattc cgcggatggc gggatcgccc tcgcgcgttt ccgaggagcc
     1141 ggccgcggca ctcactcgtg ccggatcgcg tcgatcggtg acaggcgcgc ggccttccgg
     1201 gcggggtagg tgccgaagac gaggccgacc acgatggcgg ccgcgcaggc gaccgcgatc
     1261 gactgccagg tgaacgcggc gtggacggga gcgtccgtga tcgagcggat gatccctgtg
     1321 gcgagaaggg cgcctcccat gccgaccacg accccgacga ggcttcccag gccggagatc
     1381 gccaccgatt cggccaggaa ctgcatctgt atgtcccgtg cgcgggcccc tgccgatttc
     1441 cggatgccga tctcgcgcgt ccgctcgctc acggacgcga gcaggatgtt catgatgcct
     1501 atgcctccga ccagcagcga gatggccgcg atcgaagcca gccccagctt gaagacgagc
     1561 agcccgcggc tgacctgctc cacgcgggcg cggttcgact gcacccgcac caggtccccg
     1621 ggatagcgct cggccgccca ggcaccggcc gcgcgcgcga tgccgtccac gtcctcgacc
     1681 tgccgcgcct cgagcaggat ggagggcacg cggcccgcct ggaacagcca ggcctcggtc
     1741 gaatcgttca gcggcacgta gatctcgagc gggcgggagg caggacggcc gtccagcacc
     1801 gcggcgacgg ggatcctcag acctccgatc gtcacccggc cgcccggggc gcgctccgtg
     1861 ccggacggga agaaggcctc ggccgcggcg cgtgaaagca cggcggtcac ccggtccggc
     1921 cgggcgcgcc atgcctccag gtcaccgtgc agtacgcgca cccgttcccc gagggtcgcc
     1981 gacgtgtcca ccgcccggat caggatgccg acgtcgcgcg tcgagtcggg cggccgggcc
     2041 atggccgagc ccacgagcat gcggtagcct gtgaccggca cgtcgatccg gtccaggagc
     2101 gcgtcgagat gcgcgcggga gaaatggggc agagaatcgc ggcgcaccac gatgccgtcc
     2161 gtccggacgt cggactgcac ggcgacggtc acgacgtgca ggtcggtggt ggcctcgacc
     2221 tgctcgcgcg agaagcgctc caggccgtcg ccgagcgcaa ggatggccac gagcgcggcg
     2281 acgccgatga tcacgcccag cgtcgagagc gcggaccgca tggggttgat cgccagggcc
     2341 gaaaggccgg tcaggatgga gcggatcacg gggctcgcgg tcgattcgcc acggcctacg
     2401 tcgcagtccg cgcggcgttt cacggcttgc gcggcatgga gcccgtcgat agactgcgag
     2461 ccgtcaatag catgggaggc ttcgcgtgat cccgttccag gccgtcggcc atgtccgcag
     2521 cccgtactcc gaagcggttc aggtgccccg gggccccggg gcagagcacc gcgccgaggg
     2581 cgtgatcgag gttcttcccg agttcgaggc cggcctgaag gacatcgagg gcttctcgca
     2641 cctgttcgtc atctgggttt tcgaccgcgc ggagggttac gacctcatgt cgacgccccc
     2701 tacggacgat cggtcccacg gcgtgttcgc gacccgctcg ccgcgccgcc ccaacccgat
     2761 cggcctgacg gtcgtcgagc tgctcgg
//
LOCUS       DBNHQZ010000064         2074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2726382, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000064 DBNHQZ010000000
VERSION     DBNHQZ010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2726382"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="AC16YU_01525"
     CDS             complement(<1..384)
                     /locus_tag="AC16YU_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063928.1"
                     /translation="MRGFPSRRLAMCVAALAVVFASPRVHAESPGPATSFAVLLSGGT
                     KDHADASPFYDFADPTFQFDGMQVAVRARIQLAPGTAGPLRNVTVSATLDGAPMTVHL
                     LGTDTDADGAIDLIPDARFPELSYVD"
     gene            complement(488..865)
                     /locus_tag="AC16YU_01530"
     CDS             complement(488..865)
                     /locus_tag="AC16YU_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063929.1"
                     /translation="MIEDLRFTIYQAGPLPEPTERSRPKIFRVSPGVLEARPGDEIAF
                     VSEIGEVWLMLLPEGDGGPLFEENPVRVTGGTSLRVLEGASGGYTCVVYVPAMTEEMR
                     EKDEPPRDDNLADAASPPRIIIF"
     gene            complement(896..>2074)
                     /locus_tag="AC16YU_01535"
     CDS             complement(896..>2074)
                     /locus_tag="AC16YU_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6063930.1"
                     /translation="FTHFVRGRSSLLRGDSPETLGAAIDELGRAVMLDPEYARARTAL
                     GEALLGRYRFTGDEASLRRAVSELEAAAAAGPTLAPAHVALAEAYRLVGDWDSATRAL
                     DRAAAIDSTDWQVQGALGRFHRARGRPDVADRLYRRAVALSPDYWPLRLEYGTFLFET
                     DRLQGARDQFRHIERLVPESYYAATGMGGVLYAAGRFDEAADRFRQAAEIRRSAGALS
                     NLASMLMLDGRPAEAAETYREAVKLPDGERDFRIWGNLALAEKGAGRQDRAASAFGVA
                     IELAEAERPSRPDDFSLLIDLASYNAHIGQTERAVELADGALEQARDGYSWHSAAVVF
                     ELAGNREKALYWLGRAVQSGYPVQNLAADPDLADLRRDPRYEAMLLGDVPEPLPEFDP
                     "
BASE COUNT          311 a          723 c          742 g          298 t
ORIGIN      
        1 atccacgtag gaaagctcgg gaaaccgggc atccgggatg aggtctatcg cgccgtccgc
       61 gtcggtgtca gtgcccagaa ggtgcacggt catgggagca ccgtcgagcg tcgcgctgac
      121 ggtcacgttg cgcagcggac cggccgtgcc gggcgccagc tggatgcggg cccgcacggc
      181 gacctgcatg ccgtcgaact ggaaggtcgg gtcggcgaag tcgtagaacg ggctggcatc
      241 ggcgtggtcc ttcgtccccc ccgaaagcag gacggcgaac gacgtggccg gaccggggga
      301 ctccgcgtgg actcgggggg acgcgaagac gacggcgagc gccgccacgc acatggcgag
      361 gcggcgagac gggaagcctc tcatgatgcc tgctgactgg tcgggtgaac ggggaaactc
      421 cgggcggggg ttgcccgggc catccgcgct tacgactcgt atcggcagcc tggggccgcc
      481 gcttcaatca gaaaatgatg atccgcggag gcgaggccgc atccgcgagg ttgtcgtccc
      541 ggggcggctc gtccttctcg cgcatctcct cggtcatggc gggcacgtac accacgcagg
      601 tatagccccc cgatgcgccc tccaggaccc tgagcgacgt gcctcccgtc acccggaccg
      661 gattttcttc gaagagcggg ccgccgtccc cctcggggag gagcatgagc cagacttcgc
      721 cgatctccga aacgaaggcg atctcgtctc ccggccgggc ctcgaggacg ccgggcgaga
      781 cccggaaaat cttgggccgg gagcgctccg taggttcggg gagcggcccc gcctggtaaa
      841 tggtgaatct caggtcctcg atcatggcgg ccctccgagg ggtaggtgaa cgggattacg
      901 gatcgaactc gggcaggggt tcgggcacgt cgcccaggag catcgcttcg tagcgcggat
      961 cgcggcgcag gtccgccagg tccggatccg ccgcgagatt ctgcacggga tagccggact
     1021 ggacggcacg ccccagccag tagagcgcct tctcccggtt gccggccagc tcgaagacga
     1081 ccgccgccga atgccagctg tagccgtccc tggcctgctc cagcgccccg tcggcgagct
     1141 cgacggcgcg ctccgtctgc ccgatgtgcg cgttgtacga ggccaggtcg atgagcaggg
     1201 agaagtcgtc cggtcgggac ggccgttcgg cctccgccag ctcgatcgcg acgccgaagg
     1261 ccgaggccgc acggtcctgc cgccccgccc ccttctcggc gagcgccagg ttgccccaga
     1321 tccggaagtc gcgctcgccg tccggcagct tgacggcctc ccggtacgtc tcggccgcct
     1381 cggccggcct cccgtcgagc atgagcatgc tggcgaggtt cgaaagcgcc ccggccgatc
     1441 ggcggatctc cgccgcctgg cgaaaccggt cggcggcctc gtcgaagcgt ccggccgcat
     1501 acagcacgcc acccatgccg gtagccgcgt agtagctctc cgggaccagg cgctcgatgt
     1561 gacggaactg gtcccgcgct ccctgcagcc ggtcggtctc gaacaggaag gtcccgtact
     1621 cgagccggag gggccagtag tccggcgaca atgcgaccgc gcggcggtac aggcggtcgg
     1681 cgacgtcggg tcggcccctc gccctgtgga accggcccag ggctccctgt acctgccagt
     1741 cggtggagtc gatggcggcg gcccggtcca gggcccgcgt ggccgaatcc cagtcaccga
     1801 ccagccggta cgcttccgca agggccacgt gtgccggcgc cagcgtcggc ccggcggctg
     1861 ccgctgcctc gagttcggac acggcgcgcc gaagcgacgc ctcgtctccg gtgaagcggt
     1921 acctgccgag cagggcttct ccgagcgcgg tccgcgcccg ggcatactcc gggtcgagca
     1981 ttacggcccg gcccagttcg tcgatcgcgg cgccgagcgt ctcgggcgag tcgccccgga
     2041 gcaggctcga tcggccccgc acgaaatggg tgaa
//
LOCUS       DBNHQZ010000065         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1778989, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000065 DBNHQZ010000000
VERSION     DBNHQZ010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1778989"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2719)
                     /locus_tag="AC16YU_01540"
     CDS             complement(<1..2719)
                     /locus_tag="AC16YU_01540"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6063931.1"
                     /translation="MMRLAMDDEQDRERVRGRDQVAEAVAGGPFDRDTCLVVRAGAGS
                     GKTRALVERMVALVRTGVALRNMAAITFTVRAAGEMRARFFLTLRATLRDLDVALARG
                     PDPGLQVERDRVREAAARTDDLFVDTIHSFCRTMLAERPFEAGVPPDFRQVDDREEAE
                     IRGRFWHGYLDGELRRGAHTPAWWAEIGVDPEDLSSFFGTRCEHEDLRLAPGRAERPD
                     LWPAVDETLAWLDRVLPGIPDAGADRDELTGVLHHMNRYRRLMDMDSPGSRAAFLQML
                     DVLVNDDGDLKDKARVGLWKAWGGGAYELLQSLRGKSDRPVMEPLGAFVLRTVRPAVI
                     AWKEYVYAEVREFVDGAVDAYRMHRCEEGLLTFQDLLLRAADLLRSNGDARRHFRQRY
                     THLLVDEFQDTDPVQAEIVFLLTASEDEISDWTRAHPAPGSLFLVGDDKQSIYRFRRA
                     DVQVFRQACSLIETGGGETVRFVTNFRSESRICDWVNRAVRPAFGDSEPYQAPWEDLH
                     PYLGAGTTPDPVLALDLEKENRNAGRLVARQEAGFVTAMIRRLLAGLDIPTSSGAPAR
                     LDSGPVDAGDIMILVRKATHAAEYTRALDAAAIPYVTSGGKTLGKEPVVAALLDLLEA
                     ALDPDDPVALLAVLRGSLVGASDEDLRQFRAAGGTFAFDTPGQPPSATEAFTAVRDGF
                     RLVESARRLFETYSVGEAMARLLDGEALLAGMAVRRNGSSSAGALERLLGIAREIDGA
                     GGGWIEMMDELRRLSEGEIQAEAQTLEAGCSAVRVMTVHQAKGLQARVVFLAFPLDGR
                     EHGVDFHVDRSVGEPTLYLPCLKGRAEIARPVGWDAAEQEARRFGAAEENRLVYVAST
                     RARDLLVVSRYRSKIAGPWLPLYDALESVPAVDTAALMALPFDAESHALH"
     gene            complement(2716..>3276)
                     /locus_tag="AC16YU_01545"
     CDS             complement(2716..>3276)
                     /locus_tag="AC16YU_01545"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecB family exonuclease"
                     /protein_id="HIR6063932.1"
                     /translation="EIAFGGARPGRDGARTEPYALHLDGFSVSLRGVVDRLDRLPDGR
                     LVVIDYKTGSAHGFSGHSLGDDGTKLQWALYALAVEDLFREPVERSGYLFVGGRELGL
                     ALFLSTPDRSALADRLREQAALPLLGMFPQAAGSDACRFCDFARICGDLDARKEQIES
                     KVAATPDDSDRGAALEGWKRRVKKSK"
BASE COUNT          503 a         1255 c         1069 g          449 t
ORIGIN      
        1 agtgcaacgc atgagactcc gcatcgaagg gcagagccat cagggccgcg gtatcgacgg
       61 ccgggacgga ttccagcgcg tcgtacaggg gcagccacgg ccccgcgatc ttcgagcggt
      121 accgcgatac cacgaggaga tccctcgccc gcgtggacgc cacgtacacg aggcggttct
      181 cctccgccgc cccgaaccgc cgggcctcct gttcggcggc gtcccatccg acgggccgcg
      241 cgatctccgc acgccccttc aggcacggca ggtacagcgt cggctctccg acggagcggt
      301 ccacgtgaaa atcgaccccg tgctccctgc cgtcgagcgg gaaagccagg aataccaccc
      361 gcgcctggag gcccttcgcc tggtgcaccg tcatgacgcg gacggcggag cacccggcct
      421 ccagggtctg ggcttccgcc tggatctcgc cctccgagag gcgccggagc tcgtccatca
      481 tctcgatcca tccgccgccg gccccgtcga tttcccgcgc gatgcccaga agccgctcca
      541 gggcgcccgc cgacgaggag ccgttcctgc ggaccgccat gcccgcaagc agcgcctccc
      601 cgtccaggag gcgggccatc gcctccccca cggaatacgt ctcgaacagc cgccgtgcgg
      661 actccacgag gcggaatccg tcgcggacgg cggtgaacgc ctccgtcgcc gaaggcggct
      721 gcccgggcgt gtcgaacgcg aacgtccctc ccgccgcacg gaactgcctc aggtcctcgt
      781 ccgacgcccc gacgagcgaa ccgcgcagca ccgcgagcag cgctaccgga tcgtccggat
      841 cgagcgcggc ttcgagcagg tcgaggagcg ccgccacgac gggctccttt cccagcgtct
      901 ttccgcccga ggtgacgtac gggatggccg cggcgtcgag cgccctcgtg tattcggccg
      961 cgtgggtcgc cttccggacc aggatcatga tgtcgcccgc gtccaccggc ccggaatcga
     1021 gccgcgccgg cgcgcccgac gaggtcggaa tgtcgagccc ggcgaggagc ctgcggatca
     1081 tggcggtaac gaagcccgcc tcctgtctcg cgaccagccg accagcattc cgattctcct
     1141 tctccaggtc cagcgcgagg accgggtcgg gcgtggtgcc ggcgcccagg tacgggtgca
     1201 ggtcctccca gggcgcctgg tacggctcgg aatccccgaa cgcgggacgg acggcgcggt
     1261 tgacccagtc gcagatccgc gactccgacc ggaagttggt gacgaaccgc accgtctccc
     1321 cgccccccgt ttcgatgagg gagcacgcct gccggaacac ctgcacgtcc gcccgccgga
     1381 agcggtagat ggactgcttg tcgtcgccga ccaggaacag ggacccgggc gccgggtggg
     1441 cccgggtcca gtcggagatc tcgtcctcgg acgcggtcaa caggaagacg atctccgcct
     1501 gcacgggatc ggtgtcctgg aattcgtcca cgagcaggtg cgtgtaccgc tgccggaaat
     1561 ggcgcctcgc gtcgccgttc gaccggagca ggtccgccgc gcgcagcagg agatcctgga
     1621 acgtgaggag cccttcctcg cagcggtgca tgcggtacgc gtcgacggcg ccgtccacga
     1681 actcccggac ctcggcatac acgtactcct tccaggcgat caccgccggc cggaccgtgc
     1741 ggaggacgaa ggcgccgagc ggttccataa ccggccggtc cgacttgccg cggagggact
     1801 gcaggagctc gtacgcgccg cctccccagg ccttccacag gccgaccctt gccttgtctt
     1861 tcaggtcacc gtcgtcgttt accagcacgt cgagcatctg caggaaggcc gcccgcgacc
     1921 cgggtgagtc catgtccatg agccggcggt agcggttcat gtgatggagc acgcccgtca
     1981 gttcatcccg gtccgcgccg gcatcgggaa tccccgggag cacgcggtcg agccaggcca
     2041 gcgtctcgtc cacggccggc cacaggtccg gcctctccgc ccggcccggg gcgagccgca
     2101 gatcctcgtg ctcgcaccgc gtgccgaaga acgaggagag atcctccgga tcgacgccga
     2161 tctccgccca ccacgccggg gtatgcgcgc cgcgtcgcag ctcgccgtcc aggtacccgt
     2221 gccagaaacg accgcggatc tccgcctcct cgcggtcgtc cacctgcctg aaatccggag
     2281 gcacaccggc ctcgaacggg cgctccgcga gcatcgtccg gcagaacgag tggatggtgt
     2341 ccacgaagag gtcgtccgta cgtgcggctg cctcgcggac ccggtcccgc tccacctgca
     2401 gccccggatc cgggccgcgg gcaagggcca cgtccaggtc ccgcagcgtc gcgcggagcg
     2461 tcaggaagaa gcgggcccgc atctccccag ccgcacgcac cgtgaacgtg atggcggcca
     2521 tgttccgcag cgcgaccccc gtgcgcacga gggcgaccat gcgctcgacc agcgcccgcg
     2581 tcttcccgga ccccgctccg gcgcgcacga ccaggcacgt gtcccggtcg aacggaccgc
     2641 ccgcgaccgc ctccgcgacc tggtcccggc cgcggacccg ctcgcggtcc tgctcatcgt
     2701 ccatcgccag gcgcatcatt tcgatttctt cacccttcgt ttccacccct cgagcgccgc
     2761 gccccggtcg gagtcgtccg gggtcgcggc caccttcgac tcgatctgct ccttgcgggc
     2821 gtcgaggtcc ccgcagatcc gggcaaaatc acagaacctg caggcgtcgc tgcccgccgc
     2881 ctgggggaac atccccagga gcgggagcgc cgcctgctcc cgaagccgat cggccagggc
     2941 cgacctatcg ggcgtcgaca ggaacagcgc cagccccagc tcgcgccccc ccacgaacag
     3001 gtagccgctc cgctcgaccg gctcgcggaa caggtcctcg acggcgaggg cgtacagggc
     3061 ccactgcagc ttggtcccgt catctccgag cgagtgtccc gaaaacccgt gcgcgctgcc
     3121 cgtcttgtag tcgatcacca cgagacggcc gtccggaagc cggtccagcc ggtccacgac
     3181 gccgcgcagc gagacggaaa agccgtccag gtgcagggcg tacggctccg tccgagcccc
     3241 gtcgcgcccc gggcgcgcgc ccccgaacgc gatttc
//
LOCUS       DBNHQZ010000066        17435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1373162, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000066 DBNHQZ010000000
VERSION     DBNHQZ010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 17435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17435
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1373162"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /gene="accC"
                     /locus_tag="AC16YU_01550"
     CDS             complement(<1..1257)
                     /gene="accC"
                     /locus_tag="AC16YU_01550"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6063933.1"
                     /translation="MIRKVLIANRGEIALRIIRTCHEMGLRTVAVYSTADRDSLHVRF
                     ADEAVCIGPPPSRDSYLRFDRILAAAEVTGADAIHPGYGFLAENPDFSAICEDHHIKF
                     IGPNPETIRLMGDKSLAKDPMRAAGVPVVPGSTGVVTDIAEGRDVAGGIGYPVIIKAS
                     AGGGGRGMRVVQREEDFAKQFQMASGEAEAAFGDGSVYVEKYVSSPRHIEIQVLGDGR
                     GHVVHYGERECSIQRRHQKLVEESPSPAVDAALRARMGEAATRGAMSVNYEGAGTVEF
                     LLDADGEFYFMEMNTRIQVEHPVTEEVTDTDLIAAQLHVAMGEPLKPFELHMDGHAIE
                     CRINAENPFRGFSPSPGRILTFHPPGGHGVRVDTHVYAGYVIPPHYDSMIAKLIVRGQ
                     TREMAIRKMLRALDEFVIEGVQTTIPF"
     gene            complement(1270..1794)
                     /gene="accB"
                     /locus_tag="AC16YU_01555"
     CDS             complement(1270..1794)
                     /gene="accB"
                     /locus_tag="AC16YU_01555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6063934.1"
                     /translation="MDLDRIKELLRIVAESGVSEVEIEDGDLRLIVRKNAPTVTVQPH
                     QAAYPFGWPPMAPMPQAPAPWPYPAPAAPNPAPSAAPEPAAPAPVAAARPAGVEVRAP
                     IVGTFYRSPAPDAEPYVSVGDRVKPGDVLCIIEAMKLMNEVECEMSGTVLEVLVENSQ
                     PVEFDQPLFVVDPD"
     gene            complement(1826..2398)
                     /gene="efp"
                     /locus_tag="AC16YU_01560"
     CDS             complement(1826..2398)
                     /gene="efp"
                     /locus_tag="AC16YU_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6063935.1"
                     /translation="MADTSDFRNGLTLVWNGDLWTITEFMHVKPGKGGAFVRTKLRNV
                     RTGRVVDNTFRAGEKVDTARIERRPHQFLYEDDLGLHFMNTETYEQLTLKPDAVEGRE
                     YIKEGGMIDVLVHAETDQPLATEIANSVELAVTRTDPGLKGDTATGATKPATLESGAV
                     VYVPLFINEGDVVRVNTERGEYITRVSTAP"
     gene            complement(2456..4096)
                     /locus_tag="AC16YU_01565"
     CDS             complement(2456..4096)
                     /locus_tag="AC16YU_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6063936.1"
                     /translation="MDADLASIQQARDLVRAARAAQLTARELDQEAVDRIVDAMVHAG
                     AKAADRLARLACEETGFGRPDSKKLKNLFATERLGERLRGMRTVGIVARTEDGSVWEV
                     ATPMGVIAALVPSTNPTSTAMYKAIIAAKARCGIVMSPHPRARACTAEALRVVAEAAV
                     RAGAPEGLFGCMSEITAAGTEALLEDPQTDLILATGGTAMVRAAYSKGKPAYGVGSGN
                     VPVYVDRSADLAKAAADVVYGASFDWGTLCSTERSLVVDLPVRARFLDALRSAGAHVT
                     DQAEGDLLARLVVKGGRLNTEQIGKPPFEIARMAGFSVPRDTRVLVVPAVSVGPEEPL
                     SMETLSPILTLYTADGWEAGCERCMEVLAYGGMGHTLGLHATSERVIEAFALQKPAMR
                     IVVNSVAALGSVGYTTSLFPAMTLGPGTLGGSITSDNISPMHLVNIKRVAFETRPVTG
                     PARVSPGRRPARRDPGPKADWIATVEARLRERAGNPPSPAAPPKAAPQAAAAEQRGAT
                     DQPAALPAEPAHVEPALSDEEIESLVARFTRRPGAGSN"
     gene            4333..5808
                     /locus_tag="AC16YU_01570"
     CDS             4333..5808
                     /locus_tag="AC16YU_01570"
                     /inference="COORDINATES: protein motif:HMM:NF018455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HIR6063937.1"
                     /translation="MSLSTEPSRRGVQGVPSGIEPIDREWGGLYRGGAYLVYGRAASG
                     RGLLTLRFVKAGLDIGDRCLFVASERFRDLAVEASSIGLDLRRARLQGTVRLTRVPPL
                     VNVPDDEDGAVAQAVADLAALIRRERPERVVVNDFLPFVMFRSSDRFRRAFVEMLESL
                     DGVDSTVLFALPEPANDQSARVVDFVSTKMTGTIHVEPFEYDPGSTERRLTLTPHVGH
                     FGGRVVCRWDLAALVRLPSSPATSPPARVDRGSAETAGPAPTAPAAAKEPAQPVSRPA
                     QPVSRPAPARPPAPTEPVDRQEPEEPVESAAPAAPPVEEKPPAEPAASAEPAEPAEPA
                     EPAEPAASPGPARPKPAAEPTPPDPEREAFRARLDRQFERRERGGTPFLLLAMRLDRD
                     GNGITRPFDFEFIIDLVTDSLRSQDSVLVKADAERLVVWLADSMPDDAQDFFARLRRT
                     LEAEAPQRAGQLLHAVSAIVVPDGRPFQTAAEFLAYALDED"
     gene            5813..6115
                     /locus_tag="AC16YU_01575"
     CDS             5813..6115
                     /locus_tag="AC16YU_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063938.1"
                     /translation="MDPTTCTVLLVALAALTLVGWGAWLREVNRREGPAPDAAITIAP
                     PVRAVLYVPPDAQESTSAAPATVREESPEWVMERMMDERFEPVRAPSPSDRGTDWL"
     gene            complement(6120..6881)
                     /gene="bshB1"
                     /locus_tag="AC16YU_01580"
     CDS             complement(6120..6881)
                     /gene="bshB1"
                     /locus_tag="AC16YU_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR6063939.1"
                     /translation="MADASLDVLAFAAHPDDVEFFAGGTVALLVSQGYRVGIADLTRG
                     ELGSRGTARIREREAAEAAAILGVEARVNLGLPDGDIRNTLENRVEVARVLRTFRPRI
                     LLVPAPDCRHPDHPAAARLVTEAVFPAGLRRIETPAGGESLEPWRPHHVLHYMQSIPF
                     DPTLVVDVTPVWETRVRALRAFASQVHDADRAAPGGEPETFISNPAFVDWIEARARTY
                     GYRIGATYGEPLLYRHGPIGVTDLVRCLERDLPVR"
     gene            complement(6883..9588)
                     /gene="leuS"
                     /locus_tag="AC16YU_01585"
     CDS             complement(6883..9588)
                     /gene="leuS"
                     /locus_tag="AC16YU_01585"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6063940.1"
                     /translation="MADYPFRDIEAKWQRHWEEKKTFRTPQDVDPSRPKFYVLDMFPY
                     PSGVGLHVGHPLGYIATDILARYKRMRGANVLHPIGFDAFGLPAEQFAVEHGVHPRTT
                     TEGNIANMLRQLKALGLSYDWDRVLATTDVDYYRWTQWIFLKLYGSWYDPEKGGSRPI
                     AELEEKLARERDDWADLSDAGREEVLAGYRLAYLAEVPVNWCPALGTVLANEEVTSEG
                     RSERGNHPVYRRPLRQWMLRITAYADRLERDLALVDWPEPIKLMQRNWIGRSHGATIR
                     FALPGSDDVIEVFTTRPDTLFGATYMVLAPEHPLVDRITTADRQADVTAYLEQTAART
                     EVDRMAETRTKTGVFTGGYALNPATGREIPVWIADYVLMGYGTGAIMAVPGQDERDWE
                     FAEVFGLPIVRTVQPPDDFDGKAYLGDGPAIHSGFLDGLHVAEAKAKMTAWLEAEGVG
                     RGAVTFKLRDWLFSRQRYWGEPFPVLHGPDGRIRTVDESELPVELPAMDDFSPEASDD
                     PNAPPSPPLRRAPDSWRFVERDGVGYERELNTMPQWAGSCWYYLRFIDPDNDRAFVDP
                     ERERYWMQPNGIDLYVGGVEHAVLHLLYARFWHKVLFDLGYVSTPEPFGRLFNQGYIQ
                     AFAYRDERRILVDSERVVDQDGNPALEVQDRHDRQYFFDGRPVTQEYGKMGKSLKNAV
                     SPDEINERFGCDTLRLYEMYLGPLEASKPWNTRDIVGVNRFLRRVWRVYTGEFDETLR
                     VVDAEPPEALVRSLHRTVRRVTDDLERMSFNTAIAALIEFTSELVPLGAVPRAVAEPL
                     VLLLSPLAPHMAEELWSRMGHPGSLAYEPWPVYDDALLRTETVPVAVQVNGKVRGTVH
                     VDPEAMEDAVLAAARADENVSRHLDGKTLRRAVYVPGRIVNFVVS"
     gene            complement(9698..11350)
                     /gene="bshC"
                     /locus_tag="AC16YU_01590"
     CDS             complement(9698..11350)
                     /gene="bshC"
                     /locus_tag="AC16YU_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HIR6063941.1"
                     /translation="MTLPGTETAAPTLVPFDSFGSFSTLYADYCARFDRVASFYARDW
                     TSAADRRAAVEEAAGFPRDRETLVRVLRGQQPTWGPDDTALRSVERLADPASAAVVTG
                     QQVGLFTGPLYTVLKAMTTVLLARRLEEETGRPVIPVFWLGSEDHDLDEMAGVGVPEG
                     DGVLDLRYTGHVLPPGGNLGSVGPLPFDPTIAEVVDRLETLLPPSDFHDDLVRTVREA
                     YAPGTSFRDAFGRLLRRMLPGQGLVLMSADHPELKRLALPLWTKEISDGDGLMAGLGE
                     ATARLESTYHAQVHVSPTNLFVMHGGSRLPIDLEDGRYRVRGTDQAWSRDELLRLMES
                     ETGRFSPNVVMRPLTQDLLLPTAAYVGGPGEVAYFAQYRGAYEWAGLPMPVVWPRASV
                     TLVERRVRRSMDRLGRDIPDFSGDVEALFSELVLEQLDFDLEARFGEAAGHLDRAVTG
                     LKETLTAVDPSLEKTVEATRAALAKEMERLRERVVRSERRRQEELRAHVDKVAGALFP
                     GGGFQERVVTPLWFLNKYGPDFFVRLMEQIDVGSGRHQVVDL"
     gene            complement(11394..12002)
                     /locus_tag="AC16YU_01595"
     CDS             complement(11394..12002)
                     /locus_tag="AC16YU_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR6063942.1"
                     /translation="MDYIQVDIIGLSTSPSSGGAYALVLGEVDGNRRLPIIIGAFEAQ
                     AIALELEKIQPPRPMTHDLLRDLFETLGAEVVDVVIDELREGTFFAKIRFTAGGADAQ
                     LDARPSDAVALAVRVQAPIFVAPGVLEEAGIPTEEESDVEAEPGEVDPEEHEPEEEEV
                     SSEPKSPLERIEEQLQQAISEEDYERAAQLRDEISRLKGGSN"
     gene            complement(12043..13161)
                     /gene="hisC"
                     /locus_tag="AC16YU_01600"
     CDS             complement(12043..13161)
                     /gene="hisC"
                     /locus_tag="AC16YU_01600"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6063943.1"
                     /translation="MSAHPPSDPTTIDDVVNLVRPYVRAERAYHVGSAPDIRVKLNQN
                     ESPYDLPADLKRELVEAFLDVPFNRYPSEQPDALRDAIARREGHPPDGVLVGNGSNEL
                     AYTLGLAFVGEGTPVVLPRPLFALYGTMVRIHGGRAVEVGCRPDFHFDVDGLVEAIRR
                     HAPPLTIVATPNNPTGLALPVGEIERIVEAGPGLVLVDEAYVEFNPHGSALGLLERHP
                     NVLVMRTLSKAFGLAGLRVGYLLGHPDLMAQLLKARLPFMVDRLAQAAAMALLDRPAL
                     VAERIAAIRRDTAELTRAMQALDGIEVVPSMTNFVLFRPMTPVPRFMERLADSHVLVR
                     NMGGYPELAGFLRVSTGTPGENRDFMAALKAVLGAEGR"
     gene            complement(13241..13606)
                     /locus_tag="AC16YU_01605"
     CDS             complement(13241..13606)
                     /locus_tag="AC16YU_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HIR6063944.1"
                     /translation="MENDNRDGRGRRQDTGRYRDEVYSRRVPAGKRTYFFDVKTTRSG
                     EDFFLTITESKRLDDDHYDKHKIFLYKEDFGKFVSAMLEVVGYIQEECLPDYEFRGLP
                     DLSGLQFGTDDFADDSGTA"
     gene            complement(13727..14536)
                     /locus_tag="AC16YU_01610"
     CDS             complement(13727..14536)
                     /locus_tag="AC16YU_01610"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR6063945.1"
                     /translation="MKDAAKVIRASTGVVPRLALVLGSGLNDLADSVEDAVVIDAAKL
                     PGYPRSTVQGHRGRLVFGHLEGCPVIVVQGRLHAYEGHPLDTVTLPVRLAAALGATHL
                     LLTNAAGGIRPDFAPGTLMWIVDHIDWVRRRPFDPAAGMAPGAWARPEQPPYDRAWQR
                     RLASLARGAGIATEEGTYLWTLGPSFETPAEIRLFARLGADAVGMSTVPEAEAAERLG
                     LRVLGLSTITNAAAGITGEPLNHEEVLETGSRVRDRLERIIRLVAADVAAA"
     gene            complement(14568..15593)
                     /locus_tag="AC16YU_01615"
     CDS             complement(14568..15593)
                     /locus_tag="AC16YU_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6063946.1"
                     /translation="MFRWVAAGLALVVAALLLWTFSRLVVYLVVGFVLAYLMRPVKDR
                     IQGLGTGRIPAILVTFLILFGAVAVLVRYLAPFAAEQIAAVSGQLSPGGVSRTVAERI
                     SAWLPIDPARVEDGLIRAMETLLQGDRLSRMADSVFDLFANIFYAVLVVPFVAFFFLK
                     DGSRLRKSALGAVPNRYFEITLGIIEKVEINIGRYFRAIGVQSLSIMVVSTVLLSLAG
                     LEFALAVGIFTGLANTIPYFGPLLGFAAGTVVGISQTGDFSLLPGILVAMGLTQIADN
                     VLFQPMIFSRAARAHPLVILFVVLVGAQLGGILGMLLAIPVVTTVRVVVQEIIWSYRN
                     FRSLRLA"
     gene            complement(15686..16357)
                     /locus_tag="AC16YU_01620"
     CDS             complement(15686..16357)
                     /locus_tag="AC16YU_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR6063947.1"
                     /translation="MTRRSTEEPAHTGTVTAVSAQARDANRVSVFVDGRFSFGLPADG
                     ALELGIRKGAVIDEVLLERALRLDAGYRARQRALGLLAHRPRSVEEVRGRLRRAGFDD
                     GIVDDTVRRLGDLGYLDDEAFAEAYARSRSGGRGYGPVRILSELRRKGVPEPIARRAV
                     ETLRGEGDPLDDALAVARRAWSRIRAEPDPRRRRNRMYGFLARRGFDPDVIGRVIEIL
                     DGRQP"
     gene            complement(16354..17229)
                     /locus_tag="AC16YU_01625"
     CDS             complement(16354..17229)
                     /locus_tag="AC16YU_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HIR6063948.1"
                     /translation="MRRLSTILLALAALLAGCDTVAPDRPGTLVLEAFVETDAPLPPV
                     RVSRTRSLTDTAPGAVSDATVVLTLGGRDIPYGPTGEPGTFRPTAGFDTVVARTGAAF
                     RLVVRSGEDHAQATGSVPPALTGLGLDIVIPDDPVEAVLLDSLSIPLDSLQFELPART
                     GLVYPIVATLSWAEAEADWWIALRVEPRDRFSSTLLDYFLRPSAVASESDLRDAASGS
                     GRWSGVYAVPVESDDAPVPQHDLVVTLVRGDAAWAAYAASAGDPLRREPTSNVEGGVG
                     IVAGVSVLRRTVPLP"
     gene            complement(17226..>17435)
                     /locus_tag="AC16YU_01630"
     CDS             complement(17226..>17435)
                     /locus_tag="AC16YU_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063949.1"
                     /translation="LPAYFRTDVDVSWGFAGLGAAWSVRIQLYNVLARRNVIDRTYEP
                     TDDGVSVRNRLGLPLIPLFEIRMEL"
BASE COUNT         2593 a         6086 c         6202 g         2554 t
ORIGIN      
        1 gaaggggatc gtggtctgga ccccctcgat cacgaactcg tcgagcgcgc ggagcatctt
       61 ccggatggcc atctcgcgcg tctggccgcg cacgatgagc ttcgcgatca tcgaatcgta
      121 gtgcggcgga atgacgtacc cggcgtagac gtgcgtgtcc acgcgcacgc cgtggccgcc
      181 cggcgggtgg aaggtcagga tgcggccggg cgacgggctg aagccccgga acgggttctc
      241 cgcgttgatc cggcactcga tcgcgtgccc gtccatgtgc agctcgaacg gcttcagcgg
      301 ttcacccatc gccacgtgca gctgcgccgc gatcaggtcg gtgtcggtga cctcttcggt
      361 gaccgggtgc tccacctgga tccgggtgtt catctccatg aagtagaact ccccgtctgc
      421 gtcgagcagg aactccacgg tgcccgcccc ctcgtagttg accgacatgg cgccgcgcgt
      481 cgccgcctcg cccatgcgcg cgcggagcgc ggcgtccacg gcgggggagg gcgattcctc
      541 gaccagcttc tggtgcctgc gctggatgga acactcgcgc tcgccgtaat gcaccacgtg
      601 accgcgcccg tcgcccagca cctggatctc gatgtggcgc ggggacgaga cgtacttctc
      661 gacgtacacg gatccgtcgc cgaaggctgc ctcggcctcg ccggacgcca tctggaactg
      721 cttcgcgaag tcctcttccc gctgcacgac ccgcatgccg cggccgccgc cgcccgcgct
      781 cgccttgatg atcacggggt agccgatgcc gccggcgacg tcgcgccctt cggcgatgtc
      841 ggtgacgacg ccggtcgatc ccggcacgac cggcacgccg gcggcccgca tcgggtcctt
      901 ggccaggctc ttgtcgccca tcagccggat ggtctccggg ttcgggccga tgaacttgat
      961 gtggtgatcc tcgcagatcg cgctgaagtc cgggttctcg gccaggaagc cgtagcccgg
     1021 gtggatggcg tccgcgccgg tcacctcggc ggcggcgagg atgcggtcga agcgcaggta
     1081 gctgtcgcgc gagggcggcg gaccgatgca cacggcctcg tccgcgaacc ggacgtgcag
     1141 ggaatcgcgg tcggcggtcg agtagaccgc gaccgtccgc aggcccatct cgtggcacgt
     1201 gcggatgatg cgcagggcaa tctcgccccg gttggcgatg aggaccttcc gaatcacgcg
     1261 ttgcgcggat cagtcggggt cgaccacgaa gagcggctga tcgaactcga cgggctggga
     1321 attctcgacc agcacctcga gcaccgtacc gctcatctcg cactcgacct cgttcatgag
     1381 cttcatcgcc tctatgatgc agagcacgtc gcccggcttc acgcggtcac ccacgctcac
     1441 gtacggctcg gcgtcgggag ccggcgagcg gtagaacgtg ccgacgatgg gggcacggac
     1501 ctcgacgccg gcgggtcgcg cggcggccac gggcgcgggc gcggccggtt cgggagccgc
     1561 ggagggcgcc ggattcgggg ccgcgggcgc cgggtacggc cagggtgccg gcgcctgcgg
     1621 catcggcgcc atgggaggcc agccgaacgg gtaggcggcc tggtgcggct gtacggtgac
     1681 ggtcggcgcg ttcttcctga cgatcagtcg caggtctccg tcctcgattt ccacctcgga
     1741 aaccccgctc tcggcgacga ttcggaggag ctccttgatg cggtcgagat ccatgcgttc
     1801 gaggggctgt gcggatgggg aggcgtcagg gcgccgtgga cactcgcgta atgtattcac
     1861 cgcgctccgt gttcacacgg accacgtccc cctcgttgat gaagaggggc acgtagacca
     1921 ccgccccgct ctcgagcgtg gccggcttgg tggcgccggt cgccgtgtcg cccttgagcc
     1981 cgggatccgt ccgcgtgacg gccagctcca cgctgttcgc gatctccgtc gccagcggct
     2041 ggtcggtctc ggcgtgcacg agcacgtcga tcattccgcc ctccttgatg tactcccggc
     2101 cctcgacggc atccggcttc agggtcagct gctcgtacgt ctcggtgttc atgaagtgca
     2161 ggccgagatc gtcctcgtac aggaactggt gcggacggcg ctctatccgc gcggtatcca
     2221 ccttttcgcc ggcccggaac gtgttgtcga ccacccggcc cgtgcgcacg ttccggagct
     2281 tggtgcggac gaaggccccg cccttgcccg gcttgacgtg catgaactcg gtgatcgtcc
     2341 agagatcgcc gttccagacc agggtcaggc cgttccggaa gtcgctggtg tccgccatct
     2401 gggtttcgta cggaagtaaa tagccttaca agataccgcg gcgcacggta cccggtcaat
     2461 tcgagcccgc gccggggcgg cgggtgaacc gggcgacgag actctcgatt tcctcgtccg
     2521 aaagcgcggg ctcgacgtgc gccggctcgg cgggaagggc ggccggctgg tccgtagcgc
     2581 cccgctgctc cgccgcggca gcctgcgggg cggccttcgg cggtgcggcc ggcgagggcg
     2641 gattgcccgc cctctcgcgc agccgggcct caacggtcgc gatccagtcg gccttcggcc
     2701 cgggatcccg ccgcgccggg cgcctcccgg gcgagacgcg ggcggggccc gtcacgggcc
     2761 gcgtctcgaa ggcgacccgc ttgatgttca ccaggtgcat cggcgagatg ttgtcgctcg
     2821 tgatcgagcc gccgagcgtc cccggaccca gggtcatcgc agggaacagc gacgtcgtgt
     2881 acccgacgga tccgagcgct gccaccgagt tcacgacgat tctcatggcc ggcttctgca
     2941 gcgcgaaggc ctcgatcacg cgctcgctcg tggcgtggag tccgagcgtg tgccccattc
     3001 cgccgtacgc gagcacctcc atgcagcgct cgcacccggc ctcccatccg tcggcggtgt
     3061 acagggtgag gatcggcgag agcgtctcca tggacaacgg ctcctccggt ccgaccgaca
     3121 cggccgggac cacgagcacg cgcgtatccc gcggcacgga gaagccggcc atgcgggcga
     3181 tctcgaaggg cggcttcccg atctgctccg tgttcagccg tccgcccttc acgacgaggc
     3241 gggccaggag gtcgccctcg gcctggtccg tcacgtgcgc gccggccgac cgaagggcat
     3301 ccaggaaacg ggcccggacc ggcaggtcca cgaccaggct ccgctcggtg gaacagaggg
     3361 ttccccagtc gaacgaggca ccgtagacga cgtcggccgc ggccttggcc aggtccgcgg
     3421 agcggtccac gtacacgggg acgttgcccg agccgacgcc gtaggccggc ttccccttgg
     3481 agtaggccgc gcgcaccatg gccgtgccgc cggtcgcgag gatcaggtcg gtctgggggt
     3541 cctcgagcag ggcctcggtc ccggcagccg tgatctcgga catgcagccg aacaggccct
     3601 ccggagcgcc ggcccggacg gccgcctcgg ccacgactct cagcgcctcg gccgtgcacg
     3661 cgcgggccct ggggtgcggg ctcatcacga tgccgcagcg cgccttcgcc gcgatgatgg
     3721 ccttgtacat cgcggtggac gtcgggttgg tggacggaac gagcgcggcg atcacgccca
     3781 tgggcgtggc cacctcccac accgagccgt cttccgtccg ggcgacgata cccacggtcc
     3841 gcatcccacg gaggcgctcg cccaggcgct ccgtggcgaa gaggttcttg agcttcttgg
     3901 agtccggccg gccgaagccg gtctcctcgc aggccaggcg ggccaggcgg tccgcggcct
     3961 tcgcgccggc gtgcaccatg gcgtccacga tccggtcgac ggcctcctgg tcgagctccc
     4021 gggccgtgag ctgagccgcg cgtgcggccc tgaccaggtc tctggcctgc tggatcgagg
     4081 cgaggtcggc gtccatgacg gtgcggggtg ggccggcaat atccctgtcg gccggtccgc
     4141 ggtcaagacg gcgctggcgg catcacggcg ccgggatgaa tctcaaagag ggattccgcg
     4201 tctccctatg ggccgagacc cggcgccgaa ttcgtgcgtt agagcccgta tccgggccgt
     4261 attcgatccc ggcacaccgt ttgtttcgcc agccatcgat ccctggaatc ccacttccgc
     4321 accgtccggt ccatgagtct ttctactgag ccgtcccgaa gaggagtcca gggtgttccc
     4381 tccggaatcg agccgatcga ccgggagtgg ggtgggctct accgcggggg cgcgtacctc
     4441 gtgtacggcc gtgcggcgag cggacgggga ctgctgacgc tccgcttcgt caaggccggg
     4501 ctcgacatcg gcgaccggtg cctgttcgtc gcgtcggagc ggttccggga cctggccgtc
     4561 gaggcttcgt cgatcggact cgacctccgg cgggcccgcc tccagggaac ggtgcggctc
     4621 acccgcgtgc cgcccctggt gaacgtgccg gacgacgagg acggggcggt cgcgcaggcc
     4681 gtggcggacc tggccgcgct gatccgccgg gagcgcccgg agcgcgtggt ggtcaacgat
     4741 tttctcccgt tcgtgatgtt ccggtcgtcc gaccggttcc gcagggcgtt cgtcgagatg
     4801 ctcgagtcgc tcgacggggt cgattccacc gtcctgttcg cgcttcccga gccggcgaac
     4861 gaccagtcgg cccgcgtggt ggatttcgtc tccaccaaga tgacgggcac catccacgtc
     4921 gagccgttcg aatacgatcc gggctcgacc gagcgacgcc tcaccctgac gccgcatgtc
     4981 ggccatttcg gcggaagggt cgtgtgccgc tgggacctcg cggcgctcgt gcgtctgccg
     5041 tcgtccccgg cgacgtcgcc gccggcccgg gtggaccgcg gcagcgcgga gacggccggg
     5101 cccgcgccga ccgcacccgc cgcggcaaag gagccggcac agcccgtctc tcggccggca
     5161 cagcccgtct ctcggccggc gcccgcccgt ccgccggcgc cgaccgagcc cgtcgaccgg
     5221 caggagcccg aagagcccgt cgagtccgcc gcccccgcgg cgcccccggt cgaggagaaa
     5281 ccgccggccg agcccgccgc gtcagcggag ccggccgagc ccgccgagcc cgccgagccc
     5341 gccgagcccg ccgcgtcacc cgggcccgcc cggccgaagc ccgccgccga gcctacgccc
     5401 cccgaccccg aaagggaggc gttccgcgcc cgcctcgacc ggcagttcga gcgccgggag
     5461 cgcggaggca cgccgttcct tctcctggcg atgcgcctgg accgggacgg taacggcatc
     5521 acgcggccgt tcgacttcga gttcatcatc gacctcgtca ccgacagcct gcggtcgcag
     5581 gacagcgtgc tggtgaaggc ggacgccgag cgcctggtgg tctggctggc cgacagcatg
     5641 ccggacgacg cccaggattt cttcgcgcgc cttcgccgga ccctcgaggc cgaagcgccg
     5701 cagagggcgg gtcagctgct gcatgccgtg tcggcgatcg tggtgccgga cgggcgtccc
     5761 ttccagacgg ccgcggagtt cctggcctac gccctggacg aggactgagc cgatggaccc
     5821 gaccacgtgc acggttctcc tcgtggcgct ggccgccctg accctcgtcg gctggggcgc
     5881 ctggctgcgc gaggtgaatc gccgcgaggg gccggcgccg gatgccgcga tcacgatcgc
     5941 tcccccggtc cgggcggtcc tctacgtacc cccggatgcg caggaatcca cctccgcggc
     6001 gccggccact gtccgcgagg aatccccgga atgggtcatg gaaaggatga tggacgagcg
     6061 gttcgagccc gtccgggcgc cctcgccctc ggaccgggga acggactggc tctgagagcc
     6121 taccgcacgg gcaggtcgcg ctcgaggcag cgcacgaggt ccgtcacccc gatcgggccg
     6181 tgccggtaca gcaggggttc tccgtaggtc gcgccgatcc ggtacccgta ggtgcgggcg
     6241 cgcgcctcga tccagtccac gaaggcgggg ttcgagatga aggtctccgg ctccccgcct
     6301 ggcgccgcac ggtccgcgtc gtgcacctgg ctcgcgaacg ccctgagcgc gcggacccgc
     6361 gtctcccaga ccggggtcac gtcgaccacg agcgtgggat cgaacgggat cgactgcatg
     6421 tagtgaagca cgtgatgcgg tcgccagggc tcgagggatt ctccgcccgc gggcgtctcg
     6481 atgcgtcgaa gaccggcggg gaacaccgcc tcggtgacga gccgggcggc ggccggatgg
     6541 tcggggtggc ggcagtccgg cgccgggacg aggagaatgc gcggccggaa ggtgcgaagc
     6601 acccgcgcca cctcgacacg gttctcgagc gtgttccgga tgtccccgtc gggcagtccc
     6661 aggttcacgc gcgcttcgac gcccaggatc gccgcggcct ccgcggcctc gcgctcccgg
     6721 atccgggccg tgccgcgcga tccgagctcg ccgcgggtca ggtccgcgat ccccacgcgg
     6781 tagccctgcg agacgagaag cgcgaccgtg ccccccgcga agaactccac gtcgtcggga
     6841 tgggcggcga acgccaggac gtcgagcgat gcgtcggcca tctcagctca ccacgaaatt
     6901 gacgatgcga cccggcacgt agacggcccg ccgaagggtc ttgccgtcca ggtgccggct
     6961 cacgttctcg tcggcccggg cggcggcgag gacggcgtcc tccatggcct ccggatcgac
     7021 gtggacggtg ccgcgcacct ttccgttgac ctggacggcc accgggacgg tctcggtccg
     7081 gagcagcgcg tcgtcgtaca cgggccacgg ctcgtacgcg agcgatcccg ggtgccccat
     7141 gcgcgaccac agctcctcgg ccatgtgcgg ggcgagcggc gagaggagca gcacgagcgg
     7201 ctccgcgacg gcgcgcggga cggcgccgag cggcacgagc tcggaggtga actcgatgag
     7261 cgcggcgatg gccgtgttga agctcatgcg ctccaggtcg tcggtcaccc gcctgacggt
     7321 ccggtggagc gagcgcacca gggcctccgg cggctcggcg tccaccacgc gcagggtctc
     7381 gtcgaattcg ccggtgtaca cgcgccagac gcggcgcagg aaccggttca cgcccacgat
     7441 gtcgcgcgta ttccagggct tggacgcctc gagcggaccc aggtacatct cgtacaggcg
     7501 aagcgtgtcg cagccgaagc gctcgttgat ctcgtccggg gagacggcgt tcttcaggct
     7561 cttgcccatc ttgccgtact cctgcgtgac gggccggccg tcgaagaagt actgccggtc
     7621 gtgccggtcc tggacctcga gggccgggtt tccgtcctgg tccacgaccc gctcggagtc
     7681 gaccaggatg cgccgctcgt cccggtaggc gaaggcctgg atgtaaccct ggttgaagag
     7741 gcgtccgaag ggctcgggcg tcgacacgta tcccaggtcg aagagcacct tgtgccagaa
     7801 ccgggcgtag agcaggtgca gcacggcgtg ctcgacgccc ccgacgtaca ggtctatgcc
     7861 gttcggctgc atccagtagc gctcccgctc cgggtcgacg aacgcccggt cattgtccgg
     7921 gtcgatgaac cggaggtagt accagcacga gccggcccac tggggcatcg tgttcagctc
     7981 gcgctcgtac ccgacgccgt cccgttcgac gaaacgccac gagtcgggcg cgcggcgcag
     8041 cggagggctg ggcggggcgt tcggatcgtc ggacgcctcc gggctgaaat cgtccatggc
     8101 cggaagctcc acgggcagct cggactcgtc gaccgtgcgg atgcgtccgt ccgggccgtg
     8161 caggacgggg aagggctcgc cccagtaccg ctgacggctg aagagccagt cgcggagctt
     8221 gaacgtcacc gcgccccggc ctacgccttc cgcctccagc caggcggtca tcttcgcctt
     8281 cgcctcggct acgtgcagcc cgtccaggaa accggagtgg atggccggcc cgtcgccgag
     8341 gtacgccttg ccgtcgaaat cgtccggggg ctgcacggtc cgcacgatgg gcaggccgaa
     8401 cacctcggcg aactcccagt cccgctcgtc ctgcccgggc acggccatga tggcgcccgt
     8461 accgtatccc atgagcacgt agtccgcgat ccaaaccggg atttcacgcc ccgtggccgg
     8521 attcagcgcg tatccgccgg tgaagacgcc ggtcttggtc cgggtctcgg ccatgcggtc
     8581 cacctcggtg cgcgccgcgg tctgctccag gtaggcggtg acgtcggcct gccggtcggc
     8641 cgtcgtgatc cggtccacga ggggatgctc gggcgccagg accatgtagg tcgccccgaa
     8701 gagggtgtcg ggtcgcgtgg tgaacacttc gatcacgtcg tccgagcccg gaagcgcgaa
     8761 ccggatcgtg gcgccatggc tgcggccgat ccagttccgc tgcatgagct tgatgggctc
     8821 gggccaatcg accagcgcca ggtcgcgctc cagccggtcc gcgtacgccg tgatgcgcag
     8881 catccactgc ctgagcgggc gacggtagac ggggtggttg ccgcgctccg agcggccctc
     8941 gctcgtcacc tcctcgttgg ccagtaccgt gcccagtgcg ggacaccagt tgacgggcac
     9001 ctcggccagg taggccaggc ggtacccggc caggacctcc tcgcgcccgg cgtcggagag
     9061 gtccgcccag tcgtcacgct cgcgggcgag cttctcctcg agctccgcga tggggcggga
     9121 tccgcccttc tccggatcgt accagctgcc gtacagcttc aggaagatcc actgggtcca
     9181 ccggtagtag tccacgtcgg tcgtcgcgag gacccggtcc cagtcgtagc tcagtccgag
     9241 cgccttgagc tggcggagca tgttggcgat gttgccctcg gtggtcgtcc gggggtgcac
     9301 gccgtgctcg acggcgaact gctcggccgg aaggccgaac gcgtcgaatc cgatcggatg
     9361 gaggacgttc gcgccccgca tccgcttgta ccgggccagg atgtccgtcg cgatgtagcc
     9421 cagcggatgg cccacgtgca gcccgacccc gctggggtac ggaaacatgt ccagcacgta
     9481 gaacttgggc cgggacgggt cgacgtcctg gggagtccgg aacgtcttct tctcctccca
     9541 gtggcgctgc cacttcgcct cgatatcccg gaacggatag tcggccatcg acggaaacag
     9601 gttggattgg ggctcggata agcctgaaag ctacgcgggg cctccgtgaa ttgctcgcgc
     9661 ggctcggggc gcgccgcccc cggccgggtc gccccggtca gaggtccacg acctggtgcc
     9721 tgcccgaacc gacgtcgatc tgctccatca ggcggacgaa gaagtcgggt ccgtacttgt
     9781 tcaggaacca gagcggcgtg acgacgcgct cctggaatcc gcccccgggg aagagggccc
     9841 ccgccacctt gtcgacatgt gcacggagct cctcctggcg acggcgctcc gagcggacca
     9901 cgcgctcgcg cagacgctcc atctccttcg cgagggcggc gcgtgtggcc tctacggtct
     9961 tttcgaggga ggggtcgacc gcggtgagcg tctccttgag cccggtgacg gcccggtcca
    10021 ggtggccggc cgcctcgccg aagcgcgcct ccaggtcgaa atccagctgc tcgagcacca
    10081 gttcggagaa gagcgcctcg acgtcgcccg agaaatccgg gatgtcccgc cccaggcggt
    10141 ccatcgaccg ccggacgcgg cgctccacga gcgtgaccga ggcccggggc cagacgaccg
    10201 gcatgggaag cccggcccac tcgtacgccc cgcggtactg cgcgaaatag gcgacctcgc
    10261 ccgggccgcc gacgtaggcg gcggtcggaa gcagcaggtc ctgcgtgagc gggcgcatca
    10321 ccacgttcgg gctgaaacgc ccggtctcgg attccatgag ccggagcagt tcgtcccgcg
    10381 accacgcctg gtcggtcccc cgcacgcggt accggccgtc ctccaggtcg atgggaaggc
    10441 ggctgccccc gtgcatcacg aacaggttgg tcgggctgac gtgcacctgg gcgtggtacg
    10501 tcgactccag ccgcgcggtc gcctccccga gcccggccat gagcccgtcg ccgtcgctga
    10561 tctccttcgt ccagagcggg agggccaggc gcttgagctc cgggtggtcc gccgacatga
    10621 gtaccaggcc ctggccgggg agcatgcgcc ggagcagccg gccgaaggcg tcgcggaacg
    10681 acgtgccggg cgcataggcc tcgcgcaccg tgcgcacgag gtcgtcatgg aaatcgctgg
    10741 gcggaagcag ggtttccagg cgatccacga cctcggcgat ggtcgggtcg aagggcagcg
    10801 gaccgaccga cccgaggttg cccccgggcg ggagcacgtg accggtgtag cgcaggtcca
    10861 ggacgccgtc cccctcgggc acgcccacgc cggccatctc gtccaggtca tggtcctcgc
    10921 ttccgagcca gaacacgggg atcaccggcc ggccggtctc ctcctccagg cgccgcgcca
    10981 ggaggacggt ggtcatggcc ttgagcacgg tgtacagggg gccggtgaag aggcccacct
    11041 gctgcccggt gacgaccgcc gccgacgcgg ggtcggcgag tcgctcgacg gatcgaaggg
    11101 ccgtgtcgtc cgggccccag gtcggctgct gcccgcggag cacgcgcacg agcgtctcgc
    11161 ggtcgcgcgg gaagccggcg gcctcctcga cggcggcgcg tcggtcggcc gcgctggtcc
    11221 agtcccgcgc gtagaacgac gcgacccggt cgaaccgcgc gcagtaatcg gcatagaggg
    11281 tcgaaaacga cccgaacgaa tcgaagggca ccagggtggg cgccgccgtc tcggtacctg
    11341 gaagcgtcat ctcggggggg accgacgacc gcgcggccgc atgcccgttg aagtcagttg
    11401 gatccgccct tcaggcgaga aatctcgtcg cgaagctgtg cggcgcgctc gtagtcttcc
    11461 tcggaaatgg cctgctgcag ctgctcctcg atgcgctcga ggggcgactt gggctcggag
    11521 ctgacctctt cctcctccgg ctcgtgttcc tccggatcca cctcgccggg ttcggcctcg
    11581 acgtcggact cctcctcggt ggggatgccg gcctcctcga ggacgccggg tgccacgaag
    11641 atcggggcct ggacgcggac ggcgagggcg accgcgtcgc tcggacgggc atcgagctgc
    11701 gcgtcggcgc cgccggccgt gaagcggatc ttggcgaaga aggtcccctc ccgcagctcg
    11761 tcgatcacca cgtcgaccac ttccgcaccg agcgtctcga acaggtcgcg cagcaggtca
    11821 tgggtcatcg ggcggggcgg ctggatcttc tcgagctcca gagcgatcgc ctgggcctcg
    11881 aaggccccga tgatgatggg aagtctgcgg ttgccgtcca cctctccgag aaccagcgcg
    11941 taggcgcctc cgctcgacgg gctggtcgag agcccgatga tgtccacctg gatataatcc
    12001 atcgcgttca gttctgagtc cgtggcacga ccgcggtcca gttcatcgcc cctccgcgcc
    12061 gagcacggcc ttcaacgcgg ccatgaaatc ccggttctcg ccgggcgtgc cggtcgaaac
    12121 gcgcagaaat ccggccagtt cggggtatcc gcccatgttg cggaccagga cgtgggaatc
    12181 cgccagccgc tccatgaagc gcggcacggg cgtcatcggc cggaagagca cgaagttggt
    12241 catggacggg accacctcga tcccgtcgag tgcctgcatg gcgcgggtga gctccgccgt
    12301 gtcccgccgg atggccgcga tccgctcggc cacgagggcc ggacggtcca gcagggccat
    12361 ggcggcggcc tgcgccaggc ggtccaccat gaaggggagc cgggccttga gcagctgcgc
    12421 catcaggtcc gggtgcccca ggaggtatcc gacgcgcagg ccggccaggc cgaaggcctt
    12481 cgagagcgtg cgcatcacca ggacgttggg gtgccgctcg aggagcccca gggcagagcc
    12541 atggggattg aactccacgt acgcctcgtc gacgagaacc aggccgggcc cggcctcgac
    12601 gatccgctcg atctccccga cgggaagggc gagccccgtc gggttgttcg gggtcgccac
    12661 gatcgtgagg ggcggggcgt gccgacggat cgcctccacg aggccgtcca cgtcgaagtg
    12721 gaagtcggga cggcatccga cctcgacggc gcgcccacca tggatccgga ccatcgtgcc
    12781 gtagagcgcg aaaagcggcc tgggaagcac gacgggcgtg ccttcgccca cgaacgccag
    12841 gccgagggtg taggccagct cgttcgaacc gttgcccacc agcacgccgt caggcggatg
    12901 accctcacgg cgggcaatgg cgtcccgcag cgcgtccggc tgctcggacg ggtaccggtt
    12961 gaacgggacg tcgaggaaag cctcgacaag ctcccgcttc aggtccgcag gcaggtcgta
    13021 ggggctctcg ttctggttga gcttcacccg gatgtcgggc gccgagccca cgtggtaggc
    13081 ccgctcggcc cgcacgtacg ggcggacgag attcacgacg tcgtcgatgg tggtcggatc
    13141 cgacggcgga tgcgcgctca tgcggtgcgg gacggatgct gggcgcgggt atacccggga
    13201 gggcggaagg ggtgccggcc cggatcggcg cgcggagaat tcacgccgtc ccggaatcgt
    13261 ccgcgaagtc gtcggttccg aactggaggc ccgacaggtc gggcaggccc cggaactcgt
    13321 aatccggcag gcactcctcc tggatatacc cgacgacctc cagcatcgcc gaaacgaatt
    13381 tgccgaaatc ctccttgtag agaaagatct tgtgcttgtc gtagtggtcg tcgtcgagcc
    13441 gcttgctttc ggtgatcgtc aggaagaagt cttcccccga ccgcgtggtc ttgacgtcga
    13501 aaaaataggt gcgcttgccg gcggggaccc tcctggagta cacctcgtcg cggtaacggc
    13561 ccgtgtcctg gcgccggccc cggccgtcac ggttgtcgtt ttccatctcc acgtagccgc
    13621 ccctcgtgcg gatcggccgc ggggggacgc ggccccgatt gttgcgaatc ggtgaattca
    13681 acatgcgaat atgcacaaga ggatcgcaca aatcaacgca tggccatcac gcggccgcca
    13741 cgtccgcggc gacgaggcgg atgatccgct ccaggcgatc ccggacccgg ctcccggttt
    13801 cgagcacttc ctcgtggttc aggggctctc ccgtgatgcc cgcggcggcg ttcgtgatcg
    13861 tcgacagtcc cagcactcgc aggcccagcc gctccgcggc ctcggcctcc gggacggtgc
    13921 tcatgcccac ggcgtcggcc cccagccggg cgaacaggcg gatctcggcc ggggtctcga
    13981 agctcggccc cagcgtccac aggtaggtgc cctcctccgt ggcgatgccg gctccgcggg
    14041 ccaggctcgc caggcgccgc tgccacgcgc ggtcgtacgg gggttgttcg ggccgcgccc
    14101 acgcgcccgg cgccatgccc gccgcgggat cgaacgggcg gcggcggacc cagtcgatgt
    14161 ggtccacgat ccacatcagc gtgcccggag cgaagtccgg ccggatgccg cccgccgcgt
    14221 tggtcagcag gaggtgggtc gccccgaggg ccgccgcgag gcgcacgggc agcgtcaccg
    14281 tgtccagggg atggccctcg taggcgtgca gccggccctg cacgacgatc acggggcagc
    14341 cctccaggtg tccgaacacc agccgtccac ggtgtccctg gacggtgctg cgaggatagc
    14401 ctgggagttt cgcggcatcg atgacgaccg cgtcctccac ggaatcggcg aggtcgttca
    14461 ggccggagcc gaggacgagc gcgaggcgcg gcacgacgcc ggtggaggcc cggatcactt
    14521 tggccgcgtc cttcacgaca cggtcggggg tcggcgcgtc caaaggatca ggcaaggcga
    14581 aggctccgga aattgcggta gctccagatg atctcctgca ccacgacccg caccgtggtc
    14641 acgaccggaa tcgcgagcag catgcccagg atgccgccga gctgcgcgcc gaccagcacc
    14701 acgaacagga tgacgagcgg atgtgcccgc gcggcgcgcg agaagatcat cggctggaaa
    14761 aggacgttgt ccgcgatctg ggtcaggccc atcgcgacga ggatacccgg gagcagcgag
    14821 aagtcgcccg tctgcgaaat gcccacgacg gtgccggccg cgaaaccgag cagcggaccg
    14881 aagtacggga tcgtgttggc gaggccggtg aagatgccca cggcgagggc gaactccagc
    14941 ccggccaggg agagcagcac cgtggacacg accatgatcg acaggctctg cacgccgatc
    15001 gcccggaagt agcgcccgat gttgatctcc accttctcga tgatgccgag cgtgatctcg
    15061 aagtagcggt tcggcacggc gccgagtgcc gacttccgga gccgcgagcc gtccttcaga
    15121 aagaagaagg ccacgaaggg caccacgagc accgcgtaga agatgttcgc gaacaggtcg
    15181 aagaccgaat cggccatccg ggagagccgg tccccctgga gcagggtctc catcgcccgg
    15241 atcaggccgt cctcgacgcg ggcagggtcg atggggagcc aggcgctgat ccgctcggcg
    15301 acggtccggg acaccccgcc gggcgacagc tggccggaca cggcggcgat ctgctcggcc
    15361 gcgaacgggg ccaggtaccg cacgaggacg gcgaccgcgc cgaacaggat gaggaaggtc
    15421 accaggatgg ccgggatccg ccccgttccg agcccctgga tccggtcctt gacgggtcgc
    15481 atgaggtacg cgagaacgaa acccacgacc aggtagacca cgagccgcga gaaggtccag
    15541 agcagcagcg cggccacgac cagcgcgagt ccggccgcca cccatcggaa cacgcggtcc
    15601 ggggtgatcc tgggcgcagc gggagcggga tgggcgggaa cgggcatcgg cgacggggaa
    15661 gcgggggtgt tacggccggt cggcatcacg gttgccggcc gtccaggatc tcgatcacgc
    15721 ggccgatgac gtcggggtcg aatcccctgc gcgcgaggaa accgtacatc cggtttcgtc
    15781 gccgccgcgg gtccggctcc gcgcggatgc ggctccaggc ccgacgcgcg acggcgagcg
    15841 cgtcgtccag gggatcccct tcgccccgga gcgtctcgac ggcccgtcgg gcgatgggct
    15901 ccggcacgcc cttccggcgg agctcggaca ggatccgtac gggcccgtag ccccggccgc
    15961 cggatctcga ccgggcgtag gcctcggcga aggcctcgtc gtcgaggtag ccgaggtccc
    16021 cgaggcgccg gaccgtgtcg tccacgattc cgtcgtcgaa gccggcccgc cgaaggcggc
    16081 cgcgcacctc ctcgaccgac cgcggacgat gggcgagcag tccgagggcc cgctgccgcg
    16141 cacggtatcc cgcgtcgagc cggagggccc gctccaggag cacctcgtcg atgacggcgc
    16201 ccttccggat gccgagctcg agcgcgccgt ccgccggaag cccgaacgag aaccgcccgt
    16261 cgacgaacac ggagacccgg ttggcgtccc gggcctgcgc cgagacggcg gtgacggtgc
    16321 cggtgtgggc gggctcttcc gtgcttctgc gggtcatggc agcggaacgg tccttcgaag
    16381 caccgagacg ccggccacga tgcccacgcc gccctcgacg ttggaggtcg gctcgcggcg
    16441 cagcgggtcg cccgccgagg cggcgtacgc ggcccaggcc gcgtcacccc gcacgagcgt
    16501 aacgacgagg tcgtgctggg gcacgggggc gtcgtcgctc tcgacgggca cggcgtacac
    16561 gccgctccac cggcccgatc cggaggctgc gtcccgcagg tccgactcgg acgccaccgc
    16621 ggacggccgc aggaaatagt cgaggagcgt ggatgagaag cgatcgcgcg gctccacccg
    16681 gagcgcgatc caccagtcgg cctccgcctc tgcccaggac agcgtcgcga cgatcgggta
    16741 cacgagcccg gtgcgggccg gcagctcgaa ctggagcgag tccagcggga tgctgagcga
    16801 atcgagcagc acggcctcga ccggatcgtc cggtatgacg atgtccagtc cgagaccggt
    16861 cagcgccggc ggcaccgacc ccgtcgcctg ggcgtggtcc tcgcccgagc ggaccacgag
    16921 ccggaaggcc gctccggtcc gagcgaccac cgtgtcgaag ccggccgtcg ggcggaacgt
    16981 gcccggctcg cccgtggggc cgtacgggat gtcccggccg ccgagcgtaa gcacgacggt
    17041 cgcgtcggag accgcgcccg gcgcggtgtc cgtgagcgac cgcgtccggg agacgcgcac
    17101 cggcggtagc ggggcgtccg tctcgacgaa cgcctccagc accagcgttc cgggccggtc
    17161 cggcgctacc gtgtcgcacc ccgcgaggag ggccgccagg gcgagaagaa tcgtggaaag
    17221 gcgcctcaca gctccatccg gatttcgaac agcggaatga ggggaagacc gaggcggttc
    17281 cgcaccgaga ccccgtcgtc ggtcggctcg tacgtccggt cgatgacgtt gcgccgggcg
    17341 agcacgttgt agagctggat ccgcaccgac caggcggccc cgagccccgc gaaaccccac
    17401 gacacgtcga cgtccgtgcg gaaataggcg ggaag
//
LOCUS       DBNHQZ010000067         7908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3134435, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000067 DBNHQZ010000000
VERSION     DBNHQZ010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3134435"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1035
                     /locus_tag="AC16YU_01635"
     CDS             385..1035
                     /locus_tag="AC16YU_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR6063950.1"
                     /translation="MVKKHTALMMACLLGCAGVLAFATDQPASRTRHLPLTAQFGPDR
                     FVGPRLGPIEEALLRPSRIAPLPPDMIDSETLWLARAIYSETKRPDEQMLVAWVVRNR
                     VETRYRGKSTYQDVVLDPYQFSAFLPGNVKRPYYAGLTARSQAPGWQHALRIAHAVRS
                     LDPIYRPFPAGTRHFFSERSMVDRSAPGWAHNATPVQLERPYEVDTRRFRFFADVS"
     gene            complement(1049..1693)
                     /locus_tag="AC16YU_01640"
     CDS             complement(1049..1693)
                     /locus_tag="AC16YU_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6063951.1"
                     /translation="MTLRSSALVLLFAAFSFAVALPAEAQFGIAAGLNFDSIDDINLE
                     ADDGRSTFDQATGYHAGVFYDLGVGPLGVRIGLFYRDLGTIDVEGVAEPVEVNLSMIE
                     AGVDLRYTILAVPKASPYLLVGPVVSLPRSDSEGGGAFEKSYDDALESLMVSGVVGAG
                     VSLNLGTIAIMPEIRYAVGLSGLTGKEFEIGGITYRIEETQKVKSVQLRLGVRF"
     gene            1820..2965
                     /gene="bshA"
                     /locus_tag="AC16YU_01645"
     CDS             1820..2965
                     /gene="bshA"
                     /locus_tag="AC16YU_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR6063952.1"
                     /translation="MKIGITCYPVYGGSGVVATELGKALALRGHEIHFIAYSMPFRLD
                     HFQENITFHEVHVNAYPLFEYPPYTLNLTSKMVDVVRFEQLDVLHVHYAIPHATSAVL
                     ARQILAQEGIHIPVVTTLHGTDITLVGQDPSFLPVVTWSIEQSDGVTAVSEYLRQETM
                     GCCKVDCNIKVVPNFIDTVRFRRQKKDHFKAVLSPNGERVLVHVSNFRPLKHAPEVVE
                     VFARLRAEGMPLKLLLVGDGPDRMATEHRARDLGVVDDVRFMGKQECVEEILSIADVF
                     LMPSGSETFGLAALEAMACEVPVVASNVGGLPELVVDGETGFLCPLGDIEAFTAATRR
                     ILTEDGLHDRMAAASRRRAVEEFDVHRIVPLYEEHYEAVREQAVARV"
     gene            2976..4043
                     /locus_tag="AC16YU_01650"
     CDS             2976..4043
                     /locus_tag="AC16YU_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063953.1"
                     /translation="MRHLISRKKKVYPVSDAFREYLGRYGRLQDHGIRYEELLRYANA
                     VPVYDESGEDTLWSTVLYPQFDQEEVHEALLVSYAQLKAGGDRSIIRYLYIDRVDLCV
                     YGNTLPFRIRVVNRINENFDYFYVKKVDANRLYGLEIEHILSPNRISYFVNGQTVIEA
                     HIVGVPADAFLREGMPITPFDQIRLAKDFVKFNERCFVCLLGDMHSGNFVIELTRDFE
                     KWHFRIHPIDFDQSTHHWRKEVYLPQYFPQNGPFVKVAAQHLAPENVRQYQLEERALI
                     ANRVGVSHGRFDALMSSMRLDAIAPSENVLRLGEQLAEHYDEPEFRDCSTMGDLLHTS
                     ILTTVNRPTPATAPLMGSEAS"
     gene            complement(4047..6224)
                     /locus_tag="AC16YU_01655"
     CDS             complement(4047..6224)
                     /locus_tag="AC16YU_01655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR6063954.1"
                     /translation="MTRVLRLPSALFLVAALLVPSARAQDVTGNWFGVLDPDEIGLRT
                     TLHVSATEGGYSATLDSPDQGAFGIPLDSFERVGNRVTFGLSRASVTYEGTIDEGSTV
                     IRGTLTQSDEDMPLDFGRTELEPLAGPRWATERLEKTEVYITMRDGVRLFTSIYAPRD
                     RSRTYPIIINRTPYNIEPAGEERFSGKIGSFRHFLEDGYILVLQDVRGRFMSEGTYEN
                     IRPLLREMDGPEDIDESTDTYDTIEWLVNNVEGNNGRVGVLGISYPGFYSTTSIPGAH
                     PALKAVSPQAPVTNWFLGDDWHHNGAFFMLDAFSFYVSNGVPRPEPTRAFPPPFRWPS
                     EDNYQFFLELGAIPDVVSTHLEGIEFWPELIAHPNYDDFWKDRDPRPHLTDVEPAVWT
                     VGGWFDAEDLFGPLEVYRAIERQDPDADNRLIMGPWSHGQWASGNAGNMGNVHWGRNT
                     SADFKQMELAFFNEYLKDGPDDGLAEVTVFDTGALTWDSFDVWPPGASTEATLWLQPD
                     GALGFRAPTATGSFSEYVSDPANPVPYTEDVHLRRTTAYMSDDQRFADRRPDVVTWRT
                     EPLAEAVTLTGPVEADLWVTTTGTDADFVVKIVDVFPDTLSGYPENDDGVPMQGYQML
                     VRGEIMRGRFRESFERPVPFTPGAETLVRFSVPDLHHTFKPGHRIMVQVQSSWFPLVD
                     RNPQTFVPNIFEATDEDFVKAVHRIHHDAAHPSGIRLRRLDRP"
     gene            complement(6326..6970)
                     /locus_tag="AC16YU_01660"
     CDS             complement(6326..6970)
                     /locus_tag="AC16YU_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6063955.1"
                     /translation="MDVRSFTFNPFMTNCYVCHDAAEAVIVDPSCASDEERDEVLRYV
                     REEGLAVREILLTHAHIDHIFGCAALSDAFGVGIRVHRSEVGLLAAAGEQARLFGVTV
                     DAPPEPAGFLDHGQEVRIGDARWEILHTPGHSPGSVSFVDRGNGFVIAGDVLFRDSIG
                     RTDLWQGSLPELMRSIFDRLLPLGDDMVVRPGHGPSTTIGRERRRNPFLAEGGS"
     gene            7072..>7908
                     /locus_tag="AC16YU_01665"
     CDS             7072..>7908
                     /locus_tag="AC16YU_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6063956.1"
                     /translation="MSLRMLPGPFLGWLSLLMFLLVMQFLIKYLPNLVGKGLPFQAIV
                     ELIAYNLAYMLVLAVPMSVLIAAIMTFGRLAESGAWAVMKSAGVSFVQLVWPFVVVGL
                     VLTAAMGYFNNVVLPEANFRARVLWQDIRTTRPGFELKPGVFYDWLDDYMILVRSLPA
                     DQPDRLEDVLIYDYTEWPRFRTDIKATSGTIRPVDAGRSLEIELLEGVIHRRRSMMGE
                     GGDRYERMSFDRHRMRIETPDLGFERTDPAGGRRTDRTMRSEGLRRLVDSLETSVVEH
                     RVR"
BASE COUNT         1325 a         2698 c         2589 g         1296 t
ORIGIN      
        1 cccaacccct cgaactgtgg tcgtacgtcc ggaatgccgg cgtcaacgac gatcagcggt
       61 gtcccctcac tcacggcgct ggaacggcgg aagcgattcc gctgcatttt cggcacgttg
      121 cttgtgcgaa gctacgacca agccgccgcg aatcaccagt caatcccgtt tgaacgaccg
      181 gtgaattagt atcgcgagta agtataaatt gcataattat aggtatatag cgcaagttct
      241 ccgtcggaaa cctggtattg ggcccggtgt ttgcggcgac ttcggcatgc gcccgatggc
      301 gtatcggaat gagacggggc catccccaca cgatggcggc aagccgtctc gaccccaagc
      361 ccccgcaaca acccgcaaga atccatggtg aagaagcata cggccctcat gatggcctgt
      421 cttcttggct gcgcaggagt ccttgcgttc gcgaccgacc agccggcgtc ccggacccgg
      481 catcttcccc ttaccgccca gttcggcccc gaccggttcg tgggtcctag actgggtccc
      541 atcgaggagg cgctcctccg gcccagccgg atcgcccccc tcccgcccga catgatcgac
      601 tcggagaccc tctggctcgc tcgggcgatc tactcggaaa ccaagcgccc cgacgagcag
      661 atgctcgtcg cctgggtcgt ccgcaaccgt gtcgagacgc ggtaccgcgg aaagtcgacc
      721 taccaggacg tggtgctgga cccgtaccag ttcagcgcct tccttcccgg caacgtgaag
      781 cgtccctact acgcggggct cacggccagg tcacaggctc cgggctggca gcacgcgctg
      841 cgcatcgcgc acgccgtccg ttcgctcgac ccgatctacc gtcccttccc cgccgggacc
      901 cggcatttct tcagcgagcg ttccatggtc gaccggagcg cgcccggatg ggcccacaac
      961 gcgactcccg tccagctgga gcggccgtac gaggtggata cgcggcgctt ccgcttcttc
     1021 gccgacgtct cctgacccag ggcccgattc agaagcgcac tccgaggcgc agctggaccg
     1081 acttcacctt ctgggtttcc tcgatccggt aggtgatccc gccgatctcg aactcctttc
     1141 ccgtgaggcc cgacaggccg accgcgtacc ggatttcggg catgatggcg atcgtgccca
     1201 ggttcaggct cacgcccgcg cccacgacac ccgagaccat cagcgattcc agcgcgtcgt
     1261 catacgactt ctcgaaggcg ccgccgccct cggagtcgga tctcggaagg ctcacgacgg
     1321 gacccacgag caggtaaggc gacgccttcg gcacggcgag gatggtgtag cgcagatcga
     1381 cccctgcctc gatcatggac aggttgacct cgaccggctc cgcgacgccc tcgacgtcga
     1441 tggtgccgag gtcgcggtag aagaggccga tccggacgcc gagaggcccc actcccaggt
     1501 cgtagaagac gccggcgtgg tagcctgtgg cctggtcgaa cgtgctgcga ccatcgtccg
     1561 cttccaggtt gatgtcgtcg atcgagtcga aattgaggcc ggcggcgatc ccgaactggg
     1621 cctccgccgg cagcgcgacg gcaaagctga acgccgcgaa gagaaggacc agggcagagg
     1681 agcggagcgt catggcgagc ctgttcgatg gttgtcacgc gcatgatact gcggcggcgc
     1741 ggcggcgggc aacccgattc caggggtgca acctcccccc cggtccggag ttttcgacca
     1801 tggaccaaac ggagccacca tgaagatcgg catcacctgc taccccgtgt acggcgggag
     1861 tggcgtggtc gccaccgagc tgggcaaggc gctcgccttg cgcgggcacg agatccactt
     1921 catcgcctac tcgatgcctt tccggctcga ccacttccag gagaacatca ccttccacga
     1981 ggtccacgtc aacgcgtacc cgctgttcga gtacccgccg tacacgctga acctgacgag
     2041 caagatggtc gacgtcgtcc ggttcgaaca gctggacgtg cttcacgtcc actacgcgat
     2101 tccgcatgcc acgagcgccg tgctcgcgcg gcagatcctg gcgcaggaag gcatccacat
     2161 ccccgtcgtc accacgctgc acgggacgga catcaccctg gtcggacagg atccctcgtt
     2221 cctgccggtc gtgacgtggt ccatcgagca gtcggacggc gtgaccgccg tctcggaata
     2281 cctccggcag gagacgatgg gctgctgcaa ggtggactgc aacatcaagg tggtgcccaa
     2341 cttcatagac acggtccggt tcaggcgcca gaagaaggac catttcaagg cggtcctgag
     2401 cccgaacggc gaacgggtgc tggtgcatgt gtcgaatttc cgccccctga agcatgcccc
     2461 ggaggtcgtg gaggtgttcg cccgcctgcg cgcggaaggc atgccgctga agctcctgct
     2521 cgtgggcgac gggcccgacc ggatggcgac cgagcaccgg gcccgcgacc tgggcgtcgt
     2581 cgacgacgtc cggttcatgg gcaagcagga atgcgtcgag gagatcctct cgatcgccga
     2641 cgtcttcctg atgcccagcg gctcggagac gttcggcctc gccgccctcg aggccatggc
     2701 ctgcgaggtg cccgtcgtcg cgagcaacgt cggcgggctc ccggagctcg tcgtcgacgg
     2761 cgagaccggg ttcctgtgcc cgctcggcga catcgaagcc ttcacggcgg ccacgcgaag
     2821 gatcctgacc gaggacgggc tgcacgaccg aatggcggcc gcatccaggc ggcgcgccgt
     2881 ggaggagttc gacgtgcacc gcatcgtgcc gctctacgag gagcactacg aggcggtccg
     2941 cgagcaggcg gtcgcgcggg tctgaacggg taccgatgcg ccacctcatc agccggaaga
     3001 agaaggtcta cccggtcagc gacgccttcc gggaatacct gggccggtac gggcggctgc
     3061 aggaccacgg catccgctac gaggagctgc tccgctacgc caacgccgtg cccgtgtacg
     3121 acgagagcgg cgaggacacg ctctggtcga cggtcctgta ccctcagttc gaccaggagg
     3181 aggtacacga ggcgctcctg gtctcgtacg cccagctgaa ggcgggcggc gaccggtcca
     3241 tcatccgtta cctgtacatc gaccgggtgg acctctgcgt gtacggcaac acgctgccct
     3301 tccgcatccg cgtcgtcaac cgcatcaacg agaacttcga ctacttctac gtcaagaagg
     3361 tcgacgccaa ccggctctac gggctcgaga tcgagcacat cctctccccg aaccggatct
     3421 cgtacttcgt gaacgggcag acggtcatcg aggcccacat cgtcggcgtg ccggccgacg
     3481 ccttcctgcg cgagggcatg ccgatcacgc cgttcgacca gatccggctc gccaaggact
     3541 tcgtcaagtt caacgagcgc tgcttcgtgt gcctgctcgg ggacatgcac tcgggcaact
     3601 tcgtgatcga gctcacgcgc gacttcgaga agtggcactt ccggatccac cccatcgact
     3661 tcgaccagtc cacccaccac tggcgcaagg aagtctacct gccgcagtac ttcccacaga
     3721 acgggccctt cgtgaaggtg gccgcgcagc acctcgcccc ggagaacgtg cgccagtacc
     3781 agctcgaaga acgggcgctc atcgccaacc gggtcggcgt ctcgcacggc cgcttcgacg
     3841 cactcatgtc gagcatgcgg ctggacgcca tcgcgccgtc ggagaacgtg ctccgcctcg
     3901 gcgagcagct cgccgagcat tacgacgagc cggaattccg ggactgctcg acgatggggg
     3961 acctcctcca cacgtcgatc ctcacgaccg tcaaccggcc cacgcccgcc acggccccgc
     4021 tcatgggcag cgaggcgtcc tgatgatcac gggcggtcca gtcgccgaag ccggatgccg
     4081 gaggggtgag ccgcgtcgtg gtggatccgg tggaccgcct tcacgaagtc ctcgtccgtc
     4141 gcctcgaaga tgttgggcac gaacgtctgg ggattgcggt ccacgagcgg gaaccacgag
     4201 ctctgcacct ggaccatgat ccggtgcccc ggcttgaagg tgtggtgcag gtcgggtacg
     4261 gagaagcgca cgagcgtctc cgcgccgggc gtgaagggaa cggggcgctc gaagctctcc
     4321 cggaagcggc cacgcatgat ctcgccgcgc acgagcatct ggtaaccctg catcggcacc
     4381 ccgtcgtcgt tctcggggta tccgctcagc gtgtccggga atacgtcgac gatcttgacg
     4441 acgaagtcgg cgtccgtgcc tgtcgtcgtg acccagaggt ccgcctcgac cggacccgtg
     4501 agcgtgaccg cctcggcgag gggttcggtg cgccaggtga ccacgtccgg ccgacggtcc
     4561 gcgaagcgct ggtcgtccga catgtaggcc gtcgtccggc gaaggtggac gtcctccgtg
     4621 tacgggaccg ggttcgccgg gtcggagacg tactcgctga agctgcccgt ggcggtcggg
     4681 gcccggaatc cgagcgcgcc gtccggctgc agccagagcg tggcctcggt cgatgccccg
     4741 ggcggccaga cgtcgaacga gtcccaggtg agcgctcccg tgtcgaagac ggtgacctcg
     4801 gcgaggccgt cgtcgggacc gtccttcagg tactcgttga agaacgcgag ctccatctgc
     4861 ttgaagtcgg cgctcgtatt ccggccccag tgcacgttgc ccatgttccc ggcattgccc
     4921 gacgcccact gcccgtgcga ccacgggccc atgatcagcc ggttgtcggc atccggatcc
     4981 tggcgctcga tggcccggta gacctcgagc ggcccgaaca ggtcctcggc gtcgaaccag
     5041 ccccccaccg tccatacggc cggctccacg tcggtcaggt gcggccgcgg gtcacgatcc
     5101 ttccagaaat cgtcgtaatt cggatgggcg atgagctcgg gccagaactc gatgccctcc
     5161 aggtgggtcg acaccacgtc cggaatggcc cccagctcca ggaagaactg gtagttgtcc
     5221 tcggacggcc accggaacgg gggcgggaag gcgcgggtgg gctcgggccg cggaacgccg
     5281 ttcgagacgt agaacgagaa ggcgtcgagc atgaagaagg ccccgttgtg atgccagtcg
     5341 tcacccagga accagttcgt gaccggggcc tgcggcgaga cggccttcag cgccgggtgc
     5401 gcgccgggaa tggacgtcgt cgagtagaag cccgggtacg agatgccgag cacgcccacg
     5461 cgtccgttgt tgccttcgac gttgttcacc agccactcga tcgtgtcgta cgtgtcggtg
     5521 gactcgtcga tgtcctccgg gccgtccatc tcgcgcagca gcgggcggat gttctcgtac
     5581 gtcccctcgc tcatgaaccg cccgcgcacg tcctgcagca ccaggatgta gccgtcctcg
     5641 aggaagtgcc ggaacgagcc gatcttgccg gagaaccgct cctccccggc cggctcgatg
     5701 ttatagggcg tacggttgat gatgatcgga taggtccggg agcggtccct gggggcatag
     5761 atcgacgtga agagccgcac accgtcgcgc atcgtgatgt agacctcggt cttttccagg
     5821 cgctccgttg cccagcgggg cccggccagc ggctccagtt cggtccgtcc gaagtccagg
     5881 ggcatgtcct cgtcgctctg cgtcaacgtc ccgcggatga ccgtcgaacc ctcgtcgatc
     5941 gtgccttcgt aggtgaccga cgcccgcgac aagccgaaag tcacccgatt tccgactcgc
     6001 tcgaacgaat cgaggggaat gccgaaagcg ccctggtccg ggctgtcgag cgtggccgag
     6061 tatccgccct cggtggcgga cacgtgcagc gtggtgcgga ggccgatttc gtcggggtcg
     6121 agcacgccga accagttgcc ggtcacgtcc tgggcccggg cggacggaac gaggagcgcg
     6181 gcgacgagga acagggcgga cggaaggcgg aggacgcggg tcatggcgga cgtcggtcgg
     6241 ttggaacgcc ccaccgtacg gcggacggcc atcacttcgc aaggggcggt ccgccgcgaa
     6301 ggttcgccgg ggctcccctc gcgccctacg acccgccctc cgcgaggaac gggttccgcc
     6361 gacgttcgcg gccgatggtg gtcgacggcc cgtggccggg ccggaccacc atgtcgtcgc
     6421 cgagggggag gaggcggtcg aagatcgatc gcatgagctc cggcagcgag ccctgccaca
     6481 ggtcggtgcg cccgatcgag tcgcggaaga gcacgtcgcc cgcgatcacg aagccgtttc
     6541 cgcggtccac gaacgacacg gagcccggcg agtggcccgg cgtgtgaagg atctcccacc
     6601 gggcgtcgcc gatccggacc tcctggccgt ggtccaggaa ccccgcgggc tccggcggcg
     6661 catcgacggt cacgccgaac aggcgggcct gctccccggc ggcggccagg aggccgacct
     6721 ccgaacggtg gactcggatc cctaccccga atgcgtccga gagggccgcg cacccgaaga
     6781 tgtggtcgat gtgggcgtgc gtgagcagga tctcgcgcac cgcgaggccc tcctcccgga
     6841 cgtaccgcag gacctcgtcc cgctcttcgt ccgacgcgca cgacggatcc acgatgaccg
     6901 cctccgccgc gtcgtgacag acgtagcagt tggtcatgaa cgggttgaac gtgaacgagc
     6961 ggacgtccat ggccctccgg cgcgagacgg tcgcggatcg aatggcagaa cacggggctg
     7021 gttcctacgt tcaggaccgc cacctcccga cgtatgcgtc acgttcaccg catgtccctc
     7081 cggatgctgc cgggcccgtt cctgggctgg ctgagcctcc tgatgttcct gctcgtgatg
     7141 cagttcctga tcaagtatct cccgaacctc gtcggcaagg gtcttccctt ccaggcgatc
     7201 gtcgagctca tcgcctacaa cctggcctac atgctggtcc tggcggtgcc gatgtcggta
     7261 ctcatcgccg cgatcatgac gttcggccga ctcgccgagt cgggagcctg ggcggtcatg
     7321 aagtcggccg gcgtgtcgtt cgtgcagctc gtgtggcctt tcgtcgtcgt cggcctggtg
     7381 ctcaccgccg cgatgggcta cttcaacaac gtggtgttgc cggaggccaa cttccgggca
     7441 cgcgtgctgt ggcaggacat ccggaccacc cggccgggtt tcgagctcaa gccgggcgtc
     7501 ttctacgact ggctcgacga ctacatgatc ctggtccgct cgctccctgc cgaccagccg
     7561 gaccgcctcg aggacgtcct catctacgat tacacggagt ggccgcggtt ccggaccgac
     7621 atcaaggcga cctctggcac catccgtccc gtggacgcgg gccgctcgct cgagatcgag
     7681 ctgctcgagg gcgtgatcca tcgcaggcga tccatgatgg gcgagggcgg ggaccgctac
     7741 gagcgcatga gcttcgatcg gcaccgcatg cggatcgaga cccccgacct cggcttcgag
     7801 cgcacggacc ccgccggcgg acgccggacg gaccgcacca tgcgctcgga gggccttcgc
     7861 cggctcgtcg actcgctcga gacctcggtc gtcgagcacc gcgtgcgc
//
LOCUS       DBNHQZ010000068         9751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2728217, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000068 DBNHQZ010000000
VERSION     DBNHQZ010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2728217"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4937)
                     /locus_tag="AC16YU_01670"
     CDS             complement(<1..4937)
                     /locus_tag="AC16YU_01670"
                     /EC_number="2.3.1.-"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HIR6063957.1"
                     /translation="MESPDQRAVAIVGVGAVLPDAPDAPAFWRNVASGRYSISEVPAD
                     RWDPDAYYDPDPSAPDRTYSKIGGWVQDYVWDPLGWKLPIPPRVGEAMDRAQKWAIIA
                     AREALADFGWPGRKFDTDRTAVILGSAMGGDLHYRTAQRIQFPDVMHELASAPSFASL
                     PGDVRAAVTAEVRANVATSFPPITEDTMPGELANIIAGRIANLFNFHGPNYVVDAACA
                     STMAAVTAAVEGLVQHDYDVVLSGGVDSNMSASTFTKFCKIGALSATGSRPYADGADG
                     FVMGEGAALFVMKRLADAERDGDRIYAVVRGMAGASDGKGKGITAPNPVGQRLAVERA
                     WLRAGVTPDSATMIEGHGTSTRVGDVIELEAMSSVLERYGLAPGTIPLGSVKSNIGHL
                     KGAAGAAGILKAALALHHRQIPPSINCERLNPNLDFTRSPLVVNRSLKEWDKEEGQVR
                     RAGVSAFGFGGTNFHVVLEEHVPGLLGGEGAARTTGSARVRPAAPSAARTPLRGALVV
                     GAGDVDALRNRLSEARDRVASSGAPAPIPPAASDLDAAERIAIDYGDADELLDRIDRA
                     LSALDRPDDRGWKALRARGIHRGSGPARKVAFLYTGQGSQYANMLGTLRHSVPEVREA
                     FAEADAVMAPILGRPLSEYVFIDTDDAAAVAELEEHLRQTEITQPAVLTTDEALTRLL
                     RAYGIVPDMVIGHSLGEYGALVAAGGLGFGDALRAVSARAHAMREVSMEDNGCMAAVA
                     APLDELERLLAQVDGYVVIANLNSTRQAVIGGATKAVEEAERVITEAGFAVRRVAVSH
                     AFHTRIVGPASEPLTRYLSGLDIRPPAVPVVANVTGTFYPMDPGARPAMIDLLGRQIA
                     SPVQFVSGLRTLYDQGVRVFVEVGPKRALESFAQDVLGDMEDTTTLFTNHPKTGDVVS
                     FNQALCGLYAAGLGAGTAPTRGVEAAAVPAAASEAAPRQVPPSPSSEAAPSPEPPEDR
                     VVALGRMFVDFLERSFDVYSGGASPRPRPGPAATGVVISGAALGLPGPQPVFDEGNLE
                     RILRGESFIVPIPMSVRQAMIDKHITRLVKTDAGGPRFETIDSVDEAVKLAGRPGPFD
                     LREFGVPEERIPSLNRVTQLAMAVGIEALRDAGLPLVMRYRRTTTGGRLPDRWMLPES
                     IADDTGIIFASAFPVSAAVADEVTGYDEDRALRARVAELAELRDAARRSPSPDPDLIA
                     DMDRRIDEAEGSIRELNYSVDRRLLFRILSMGHSQFAEHIGARGPNTQVNSACASTPT
                     AVSIAEDWIQAGRCRRVVIVSADDVTGGRLLEWIGAGFLASGAAATDERVEDAALPFD
                     RRRHGMVVGMGAAALVVEAPDAVAERGMQPICEVIGAIARNSAFHGTRLDVRHIAGVM
                     EELVSAAERRWGIDRRAIAPETVFVSHETYTPARGGSAQAEIDAIRAVFGDRADAIVM
                     ANTKGFTGHAMATGIEDVLAVRTLETGIVPPVANFREVDPDLGALNLSRGGSYPVRYS
                     LRLGAGFGSQIAMMLLRWVPTADGRRRTPAELGPDYRTVDPARRRAWLRAASGYNDPV
                     LEVVGRTLRIRDDGPPASRPPAPSPVASVSAEQSVPAPAPAPASSGDGAATPAPVAAS
                     GTDAVRQRVLDIVSGVTGYPPDMLDPDLDLEADLG"
     gene            complement(4973..6646)
                     /locus_tag="AC16YU_01675"
     CDS             complement(4973..6646)
                     /locus_tag="AC16YU_01675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6063958.1"
                     /translation="MQTAETARRTGLDRADLDLVLESIREFAAEALPDARLLELDHAD
                     EFPEATVRDLCGEGLGIHLLFVPEAYGGMGGSTFDVYRVCEALARVDLGIATGVFATF
                     LGSDPIVFGGTEEQQAYWLGRIASEGLLMAYAATEPQAGSDLGALRSTAVPVEEDGTV
                     TAYRLNGSKQWISNGGYADACTFLAKAPGGPSWFVVERDAPGLSHGSPEDKHGIRLSN
                     TSTVTCDDVVVPADRLIGGVEGQGLLQAQAVFGYTRLMVAAFGLGGGWAALDRAIPYS
                     KDRIQAGSPLSEKEGYTHKLIVPHVVRLEASRAYIEDTARRIDAGEGQLNTEGAIAKY
                     LATEFGNAAADASIQALGGYGYTREYMVEKIRRDIRITTIYEGTSEIMEMTIARDRWQ
                     LHLKTRGGYYAERAGALRAIAAGNDESGALAAADALDALSTLLEQARVERLTRNQHVL
                     FRIGELIAWVETAEVFARRARTALAGELPEKADRRFEPRVLAAMSRVFAREAALRVVT
                     DGLRWITGSSASDAGAFASRLSAERIYGAQAGVLDDLQLVADALYHRNH"
     gene            complement(6916..7803)
                     /locus_tag="AC16YU_01680"
     CDS             complement(6916..7803)
                     /locus_tag="AC16YU_01680"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR6063959.1"
                     /translation="MPASPPALSAADGRPIRAARLLLAALLLAAPACSGSDPNGGGDG
                     DDIPTKEGWSLAWHEEFDAATVDLSTWNFWVTGNPYNNELQYYTNRPQNARIEDGKLV
                     IEAHGEEFTGPDGTRAYTSARMNTQGKADFLYGRFEIRARLPFGQGLWPAIWMMPTYS
                     EYGGWPRSGEIDIMELLGHEPHKTYGTIHHTDSNGAHAYSGGSYTLPSGTFFETFHTF
                     ALEWEEGVMRWYVDDTLILTKTSWGTEGHDFPAPFDRHFHLILNVAVGGDWPGSPNAT
                     TIFPQRMEVDWIRVFERAE"
     gene            complement(7816..8517)
                     /locus_tag="AC16YU_01685"
     CDS             complement(7816..8517)
                     /locus_tag="AC16YU_01685"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR6063960.1"
                     /translation="MTGPGRPYRGILAAGLGALALAGCAGALELRDRPIPFTDARIAM
                     TREYAAERYGLSDALRIDPRVIVLHWTAIATLEDSWGAFVPETLPGARADLAGAGAVN
                     VSIQFLVDRDGTVYRLMPETWMARHTIGLNHAAIGVENVGGAGSVDDLTDAQIRANIR
                     LVRYLKAKYPAIGYLIGHHEYTEFEGHPLWLERDPDYRTEKVDPGVRFMTAVRRGVND
                     LSLRGPADIRAEIAR"
     gene            complement(8514..9704)
                     /gene="kbl"
                     /locus_tag="AC16YU_01690"
     CDS             complement(8514..9704)
                     /gene="kbl"
                     /locus_tag="AC16YU_01690"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR6063961.1"
                     /translation="MFQSTAPQLATVLDEIRSSGLYKDERVITSQQAAEIEVAGGREV
                     INFCANNYLGLANHPDLLEAAQEGIRRYGFGLASVRFICGTQDVHKELEARIAEFLGT
                     DDTILYSSCFDANGGLFETLLDEDCAIISDQLNHASIIDGIRLCKADRHRYPHGDMDA
                     LESALKATQGARRRMIATDGVFSMDGSVAKLDEICDLADRYGAMVMFDDCHATGFFGP
                     TGRGTHEYRGVMDRVDIITSTLGKALGGASGGFTTGRKEAIDLLRQRSRPYLFSNSLA
                     PVIAYTSVKVLDMLSGTTALRDRLEESTMYFRKRMTANGFDIKPGVHPIVPIMLYEAR
                     LAADMARDLLEEGIYVIGFSYPVVPKGEARIRVQVSAAHTREHLDQAIDAFAAVGRRH
                     GVIA"
BASE COUNT         1363 a         3400 c         3544 g         1444 t
ORIGIN      
        1 atgccgaggt cggcctcgag gtccagatcg ggatcgagca tgtccggcgg gtagccggtg
       61 acgccggaga cgatgtccag cacgcgctgc cggacggcgt ccgtgccgga ggccgcgacc
      121 ggtgccggcg tggcggcccc gtcgcctgag gaggccgggg cgggcgccgg ggcgggaacg
      181 gactgctcgg ccgatacgga ggcaacgggg ctcggggcgg gcgggcgcga cgcgggaggc
      241 ccgtcgtcgc ggatccgcaa tgtgcggccg acgacctcca ggacggggtc gttgtacccg
      301 gaggccgcgc gcagccacgc ccgtcgccgg gcggggtcga ctgtgcggta gtccgggccc
      361 agctcggcgg gcgtccgccg ccgcccgtcc gcggtcggca cccagcgcag cagcatcatc
      421 gcgatctgcg atccgaagcc cgcccccagg cgaagcgaat accggacggg atacgagccg
      481 cccctggaca ggttgagcgc cccgaggtcc gggtcgacct cgcggaaatt cgcgaccggc
      541 ggcacgatgc cggtctcgag cgtccggacc gcgagcacgt cctcgatgcc cgtggccatg
      601 gcgtggccgg tgaatccctt ggtgttggcc atcacgatgg cgtccgcgcg gtcgccgaag
      661 acggcccgga tggcgtcgat ctcggcctgc gcgctgcccc cgcgcgcggg cgtgtacgtc
      721 tcgtgggaga cgaagacggt ctcgggcgcg atcgcccgcc ggtcgatgcc ccagcggcgt
      781 tcggcggccg acacgagctc ctccatgacg cccgcgatgt gccgcacgtc gaggcgcgtg
      841 ccgtggaagg ccgagttgcg ggcaatcgcc ccgatgacct cgcagatcgg ctgcatgcct
      901 cgctcggcga cggcgtccgg cgcctcgacc acgagcgccg cggcgcccat gcccacgacc
      961 atgccgtgcc tgcgccggtc gaagggcagg gcggcgtcct cgacccgctc gtccgtggcg
     1021 gccgctccgg aggcgaggaa gccggcgccg atccattcca ggagccgtcc gcccgtgacg
     1081 tcgtcggccg agacgatcac gacccggcgg caccgtcccg cctggatcca gtcctcggcg
     1141 atggagacgg cggtcggcgt gctcgcgcag gccgagttga cctgcgtgtt ggggccgcgc
     1201 gcgccgatgt gctcggcgaa ctgcgagtgg cccatggaca ggatccggaa gagcagccgc
     1261 cgatccacgg aatagttcag ctcgcggata ctgccttccg cctcgtcgat cctgcggtcc
     1321 atgtccgcga taagatccgg gtcgggggag ggcgaccgcc gggcggcgtc ccggagctcg
     1381 gccagctcgg caacgcgcgc gcgcagggcg cggtcctcgt cgtacccggt gacctcgtcc
     1441 gcgacggccg cggagaccgg gaaggcggag gcgaagatga tgccggtgtc gtcggcaatc
     1501 gattcgggaa gcatccaccg gtccggcagc cgtccgcccg tcgtcgtccg gcggtaccgc
     1561 atcacgagcg gcagcccggc gtcccgcagg gcttcgatgc cgacggccat ggcgagctgc
     1621 gtgacccggt tgagcgacgg gatccgctct tcggggaccc cgaactcgcg aaggtcgaac
     1681 ggcccggggc gtccggccag cttgacggcc tcgtccaccg aatcgatggt ctcgaaccgg
     1741 ggcccgccgg cgtccgtctt caccaggcgc gtgatgtgct tgtcgatcat cgcctgccgc
     1801 acgctcatgg gaatcggcac gatgaacgac tccccgcgca ggatgcgctc caggtttccc
     1861 tcgtcgaaca cgggctgcgg cccggggagg ccgagcgcag cgccggagat gacgacgccc
     1921 gtcgccgcgg gcccggggcg cgggcgcgga gacgcgccgc ccgagtagac gtcgaacgac
     1981 cgctccagga agtccacgaa catgcggccg agggccacca cgcggtcctc gggcggctcc
     2041 ggggacggcg ccgcctcgga cgacggcgac ggcggcacct gccggggcgc cgcctcggac
     2101 gccgcggcag gcaccgcggc agcctcgaca ccgcgggtcg gggccgtgcc cgcgccgagc
     2161 ccggccgcgt acagcccgca gagcgcctgg ttgaacgaga cgacgtcgcc cgtcttcggg
     2221 tggttggtga agagcgtcgt cgtgtcctcc atgtctccga ggacgtcctg ggcaaagctc
     2281 tccagcgccc gcttcggccc gacttccacg aagacccgga cgccctggtc gtagagcgtc
     2341 cggagcccgg agacgaactg gaccggcgag gcgatctgcc ggccgaggag gtcgatcatc
     2401 gcgggccggg ccccgggatc catgggatag aacgtccccg tcacgttggc gaccaccggc
     2461 acggccggcg gacggatgtc aagccccgac aggtagcgcg tgagcggttc cgaggcgggc
     2521 cccactatcc gggtgtggaa ggcgtggctc acggcgacgc ggcggacggc gaaccccgcc
     2581 tccgtgatga cccgctccgc ctcctccacg gccttcgtcg ctccgccgat cacggcctgc
     2641 cgcgtcgagt tcaggttggc gatgaccacg tagccgtcca cctgcgcgag cagccgctca
     2701 agctcgtcga gcggcgcggc gacggccgcc atgcagccgt tgtcttccat cgagacctcg
     2761 cgcatggcat gcgcgcgggc gctcacggcg cgaagcgcgt ccccgaatcc gagccccccg
     2821 gcggccacca gggcgccgta ctcgccgagg ctgtgcccga tcaccatgtc cgggacgatg
     2881 ccgtacgccc ggaggagacg ggtgagcgcc tcgtcggtcg tgagcacggc gggctgggtg
     2941 atctcggtct ggcggaggtg ctcctcgagc tccgcgacgg cggccgcgtc gtccgtgtcg
     3001 atgaagacgt actccgagag cggcctgccc aggatgggcg ccatcacggc gtccgcctcg
     3061 gcgaacgcct cccgcacttc gggcaccgag tgccggaggg tccccagcat gttggcatat
     3121 tgggagccct ggcccgtgta caggaaggcc accttgcggg ccggcccgct gccccggtgg
     3181 atgccccgtg cgcgcagcgc cttccagccc cggtcgtcgg ggcgatcgag cgcactcagc
     3241 gcccggtcga tgcggtccag gagctcgtcc gcatcgccgt agtcgatggc gatccgctcg
     3301 gcggcgtcga ggtcggaggc cgcgggcggg atcggcgccg gggcgcccga cgaggccacg
     3361 cggtcgcgcg cctcagacag gcggttgcga agcgcatcca cgtcgcccgc gccaacgacg
     3421 agcgctcccc gaagcggcgt gcgcgccgct gagggcgcgg cgggacgcac ccgggcggag
     3481 cccgtcgtcc gggcggcgcc ctcgccgccg agaaggccgg gcacgtgctc ctcgaggacc
     3541 acgtggaaat tcgtccctcc gaagccgaag gccgacaccc cggccctgcg gacctgtccc
     3601 tcctccttgt cccactcctt caggctgcga ttgacgacga gcggcgaccg ggtgaagtcg
     3661 agattcgggt tcagccgctc gcagttgatg ctgggcggaa tctgccggtg gtgcagggcg
     3721 agggccgcct tgaggatgcc ggccgcaccg gccgcgccct tcaggtggcc gatgttcgac
     3781 ttgacggaac cgaggggaat ggtgcccggg gcgagcccgt agcgctcgag gacggacgac
     3841 atggcctcga gctcgatcac gtcgcccacc ctggtcgacg tgccgtggcc ctcgatcatg
     3901 gtcgcggagt cgggagtgac gcccgcgcgc agccaggcgc gctcgacggc caggcgctgt
     3961 ccgaccgggt tcggcgcggt gatgcccttc cccttgccgt cgctcgcgcc ggccatgccg
     4021 cggacgacgg catagatccg atcgccgtcc cgctcggcgt ccgcgaggcg cttcatcacg
     4081 aacagggccg cgccttcgcc catcacgaag ccgtcggcac cgtccgcgta cggcctcgag
     4141 cccgtggccg aaagagcgcc gatcttgcag aacttggtga aggtcgacgc gctcatgttc
     4201 gaatcgacgc cgcccgagag cacgacgtcg tagtcgtgct gcaccaggcc ctccaccgcc
     4261 gccgtgaccg ccgccatcgt ggaggcgcac gccgcgtcga ccacgtagtt cggcccgtgg
     4321 aaattgaaga ggttcgcgat gcggcccgcg atgatgttcg cgagctcgcc cggcatcgtg
     4381 tcctcggtga tgggagggaa gctcgtggcc acgttggccc ggacctcggc ggtgaccgcc
     4441 gcacggacgt caccgggaag cgaggcgaac gacggcgccg aggccagctc atgcatcacg
     4501 tcgggaaact ggatgcgctg cgccgtccgg tagtgcaggt cgccgcccat cgcgctgcca
     4561 aggatgacgg ccgtgcggtc cgtgtcgaac ttgcgcccgg gccagccgaa gtcggcgagc
     4621 gcctcgcggg cggcaatgat ggcccatttc tgcgccctat ccatcgcctc gccgaccctg
     4681 ggcgggatgg gcagcttcca tccgaggggg tcccacacgt agtcctgcac ccacccgccg
     4741 atcttcgagt aggtccggtc cggggccgag ggatccgggt cgtagtaggc gtcggggtcc
     4801 caccggtccg cgggcacctc ggaaatgctg taccgtccgg aggcgacgtt ccgccagaag
     4861 gccggagcgt ccggggcatc cggcaggacg gcgcctacgc cgacgatggc tacggctcgc
     4921 tggtcagggc tctccatggc aggttcgggc aatcgggcgt gaatcgtgca gttcagtggt
     4981 tccggtggta gagggcgtcg gcgacgagct gcaggtcgtc gagcacgccg gcctgcgcgc
     5041 cgtagatgcg ctcggcggac aggcgggacg cgaaggcgcc ggcgtcggac gccgaggagc
     5101 cggtaatcca ccggagcccg tcggtgacga cgcggagggc ggcctcccgg gcgaacaccc
     5161 ggctcatggc ggccagcacg cggggttcga accgtcggtc cgccttctcg ggcagctcgc
     5221 ccgcgagggc ggtccgtgcg cggcgggcga aaacctcggc ggtctccacc caggcgatga
     5281 gctcgccgat ccggaagagc acgtgctggt tgcgggtgag gcgctcgacc cgcgcctgct
     5341 cgaggagggt ggacagggcg tcaagggcgt cggccgcggc cagggcgccc gactcgtcgt
     5401 ttccggccgc gatcgcgcgg agcgctcccg cccgctcggc gtagtagccg ccgcgggtct
     5461 tcaggtgcag ctgccagcgg tcgcgggcga tggtcatctc catgatctcg gaggtgccct
     5521 cgtagatcgt ggtgatccgg atgtcgcgcc ggatcttctc gaccatgtac tcgcgggtgt
     5581 acccgtagcc tccgagtgcc tggatggacg cgtccgccgc cgcgttcccg aactcggtcg
     5641 cgaggtactt ggcgatggcg ccctccgtgt tcagttggcc ctcgcccgcg tcgatccgcc
     5701 gggcggtatc ctcgatgtag gccctggacg cctccaggcg cacgacgtgg gggacgatga
     5761 gcttgtgggt gtagccctcc ttctcggaga ggggagaccc ggcctggatg cggtccttcg
     5821 agtaggggat ggcgcggtcg agggcggccc atccgccgcc gagaccgaac gcggcgacca
     5881 tcagccgcgt gtagccgaat accgcctgcg cctgcagcag gccctggccc tcgacgccgc
     5941 cgatcaggcg gtcggccggg accacgacgt cgtcgcacgt cacggtcgac gtgttgctga
     6001 gccgtatgcc gtgcttgtcc tcggggctgc cgtgggagag ccccggcgcg tcgcgctcga
     6061 cgacgaacca ggacgggccg cccggcgcct tcgcgaggaa cgtgcaggcg tccgcgtatc
     6121 cgccgttcga gatccactgc ttcgagccgt tgagccggta ggcggtgacg gtcccgtcct
     6181 cctcgaccgg gacggcggtg gaccggagcg cccccaggtc cgatcccgcc tgcggctcgg
     6241 tcgccgcgta ggccatgagg aggccctcgc tcgcgatgcg tcccagccag taggcctgct
     6301 gctcctccgt tccgccgaac acgatcgggt cgctgccgag gaacgtcgcg aagacccccg
     6361 tggcgatgcc caggtccacg cgcgcgaggg cctcgcatac ccggtagacg tcgaacgtgc
     6421 tgccgcccat gccgccgtac gcctcgggga cgaacaggag gtggatgcca agcccctcgc
     6481 cgcacaggtc gcggacggtc gcctccggaa actcgtcggc atggtcgagc tccagaagac
     6541 gggcgtccgg cagcgcctcg gccgcgaact cgcggatgga ttccaggacc aggtccaggt
     6601 cggcacggtc gaggcccgtc cggcgcgcgg tttcggctgt ctgcatcacg tttggacgcg
     6661 gttcggcagg gtacacgttg agccgtcaaa gaacccgccc ggcgcccgaa acgggtagag
     6721 gcgttcacct acagcctgtg tagggccgcc gggcggaacc ctgtcggggc gccccctaca
     6781 cgatcgccac gcttccgccc ggcggccgga tacggggcct tccgcccact ttccggccgc
     6841 aggatcgccg ccgcccggag acgccgcgcc gtcggggcgc cccgcaggcc gggcgcgggt
     6901 cgatccccgc cgcggctact cggcgcgctc gaacacccgg atccagtcga cctccatccg
     6961 ctgcgggaag atggtggtcg cgttcgggct gcccggccag tcgccgccca ccgcgacatt
     7021 caggatcagg tggaaatgcc ggtcgaaggg ggcggggaag tcgtgcccct cggttcccca
     7081 cgacgtcttc gtgaggatca gcgtgtcgtc cacgtaccag cgcatcacgc cctcctccca
     7141 ctcgagcgcg aacgtgtgga acgtctcgaa aaaggtgccc gacggcagcg tgtacgagcc
     7201 cccgctgtac gcgtgcgcgc cgttcgagtc ggtatggtgg atcgtcccgt acgtcttgtg
     7261 cggctcgtgt cccagtagct ccatgatgtc gatctcgcca gagcgcggcc agccgccgta
     7321 ctccgagtac gtgggcatca tccagatcgc cggccacagg ccctggccga acggcagccg
     7381 cgcccggatc tcgaaccggc cgtacaggaa atccgccttc ccctgcgtgt tcatccgggc
     7441 ggacgtgtac gcgcgggtcc cgtcggggcc cgtgaactcc tccccgtggg cctcgatcac
     7501 gagcttgccg tcctcgatgc gcgcgttctg cgggcggttc gtgtagtact gcagctcgtt
     7561 gttgtacgga tttcccgtga cccagaagtt ccaggtcgag aggtcgacgg tggccgcgtc
     7621 gaattcctcg tgccaggcga ggctccagcc ctccttggtc gggatgtcgt cgccgtcccc
     7681 gccgccgttc ggatcggagc ccgagcacgc gggagcggca agcagaaggg ccgcgagaag
     7741 cagccgggct gcccggatcg ggcggccgtc ggcggcggag agggcggggg gcgatgcggg
     7801 catggggatg cggattcagc gggcgatttc ggcccggatg tcggcggggc cacgcagcga
     7861 gaggtcgttc acgccgcgcc gcacggccgt catgaatcga acgccgggat ccaccttttc
     7921 ggtccggtag tccggatcgc gttcgagcca gagcgggtgg ccctcgaact cggtgtattc
     7981 gtggtgtccg atgaggtagc cgatcgccgg gtacttcgcc ttcaggtagc gcaccaggcg
     8041 gatgttcgcg cggatctggg cgtcggtcag gtcgtcgacc gagcccgcgc ctcccacgtt
     8101 ctcgacgccg atggccgcgt gattcagccc gatggtgtgc cgggccatcc aggtttcggg
     8161 catcagccgg tagacggtgc cgtcccggtc caccaggaac tggatcgaca cgttgaccgc
     8221 gcccgcgccg gcgaggtcgg cccgcgcgcc gggaagcgtc tcggggacga acgcgcccca
     8281 cgagtcctcg agcgtcgcga tggccgtcca gtgcagcacg atcacgcggg gatcgatgcg
     8341 cagggcgtcg ctgagcccgt aacgctcggc cgcgtattcg cgggtcatcg cgatgcgggc
     8401 gtcggtgaac gggatcgggc ggtcgcggag ttcgagcgcg ccggcgcatc ccgccagggc
     8461 gagcgcaccg agcccggcgg cgaggattcc gcggtatggc cgcccggggc ccgtcatgcg
     8521 atcacgccat gcctgcggcc gacggccgcg aaggcgtcga tcgcctggtc caggtgctcc
     8581 cgcgtgtggg cggcggatac ctggacccgg atgcgggcct cgcccttcgg gacgaccggg
     8641 taactgaacc cgatcacgta aatgccctcc tccaggaggt cccgcgccat gtcggcggcg
     8701 aggcgcgcct cgtacagcat gatcggcacg atgggatgca cgccgggctt gatgtcgaag
     8761 ccgttcgccg tcatccgctt ccggaagtac atcgtgctct cctcgaggcg gtcgcgcagg
     8821 gccgtcgtcc cggagagcat gtccagcacc ttgacgctgg tgtacgcgat gacgggtgcg
     8881 agcgagttcg agaagaggta cgggcgcgac cgctggcgca gcaggtcgat cgcctccttc
     8941 ctgccggtcg tgaatccccc cgaggccccg ccgagcgcct tgccgagcgt cgacgtgatg
     9001 atgtccacac ggtccatcac gccgcggtac tcgtgcgtgc cgcgaccggt cgggccgaag
     9061 aagcccgtgg cgtggcagtc gtcgaacatc accatggcgc cgtagcggtc cgcgaggtcg
     9121 cagatctcgt cgagcttcgc cacggacccg tccatcgaga agaccccgtc cgtcgcgatc
     9181 atccgccgcc gagccccctg cgtggccttg agggcgctct cgagcgcatc catgtcgccg
     9241 tgcgggtacc ggtgccggtc ggccttgcac aggcggatgc cgtcgatgat ggaggcgtgg
     9301 ttgagctggt cgctgatgat cgcgcagtcc tcgtcgagca gcgtctcgaa cagcccgccg
     9361 tttgcgtcga agcatgagct gtacaggatc gtgtcgtccg tgcccaggaa ctccgcgatg
     9421 cgggcctcga gctccttgtg gacgtcctgc gtcccgcaga tgaaacggac cgaggccagg
     9481 ccgaatccgt accggcggat tccctcctgg gccgcttcca gcaggtcggg gtggttggcc
     9541 agcccgaggt agttgttggc gcagaagttg atgacctcgc gtccgccggc gacttcgatc
     9601 tcggcggcct gctgggaggt gatgacccgc tcgtccttgt acagtcccga ggagcggatt
     9661 tcgtcgagca cggtggcgag ctggggcgct gtcgattgaa acatggctcc ggtatccggt
     9721 atgggagaga gctcaggcca ccgtttcggg c
//
LOCUS       DBNHQZ010000069         7292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_20037, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000069 DBNHQZ010000000
VERSION     DBNHQZ010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_20037"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="AC16YU_01695"
     CDS             <1..430
                     /locus_tag="AC16YU_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6063962.1"
                     /translation="FLACVLFTWVILSRLRLGRHIYAVGGNEEAARLSGVPVRRTLIL
                     AYALSGLFSAVAGICQAAQETQGDPETGMAYELDAIAMVVIGGTTLAGGKGSVILTLI
                     GVLTIGYLQKILSLNAAPEATRLMLTGAIIIIAVLFQRGR"
     gene            434..1375
                     /locus_tag="AC16YU_01700"
     CDS             434..1375
                     /locus_tag="AC16YU_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6063963.1"
                     /translation="MRYLPVLFVLLAACGSPGPRGHAGTAEDPWVIGMSQCNLGEPWR
                     VQMDADVREAAAAHDNLRVVFKDAQNNALTQRAQVEEFVEQGVDLLIISPKEAAPLTA
                     PVAEAYRRGIPVIVLDRRVQGDAYTVFIGADNVRIGREAGRWIRETLVGEGRIVELKG
                     LMTSTPGQDRHSGFLEGLDLESNPGLEIVFEGEMQWLEPNARREMESALATNDRIDAV
                     YAHNDPGAHGAWLAARAAGREDQMLFVGIDALPHEGRQYVAQGIMDATFLYPTGGAEA
                     IDAALTLLGGGTVPKEIVLGTRLFTGENLEDGGEAIE"
     gene            1388..2905
                     /locus_tag="AC16YU_01705"
     CDS             1388..2905
                     /locus_tag="AC16YU_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HIR6063964.1"
                     /translation="MKQRIALFWPGDGRDIPNELAVPSATEATRQMEAALRRLDREPY
                     RIEGWLTKPHHAIERLGPVRDPMIGICVHWFYGPHTTDGVVGKDNPLLLASNFSGQWP
                     GLVGLLNTGACLASLNRPFSRVWTDAEDWTRDDLFMSRLEQWCEEGSIVYDVDALHFP
                     TSVPPGAAELASKVARGFRDRRALILMLGDTSMGMINGYFGPRLLAPHGFAEHKIDQA
                     WIVDRGSRIADGRIDDAFRFVQDRGVTFHWRETGAEDFTEDSTREQLRDYLAVLDMAD
                     EYRADCIGWQYQLGLIPLRPPSDFAEGLLNSTCRPESNGDTIVTATEADQGNAVPMEM
                     MKRLLKAKGLHQSVAFHDVRWGGEHEGRFIWVLLNSGSTGAFPFNHDPDTLEGAHSYR
                     QAAIYFPFAGGTLTGESLPGRVTWSRAYIRNDQMWMDIGRGEVVSLPPKKRDAWWNGT
                     NRQWPFMAADLGIGRDTLMAHYMSNHIAVAYGDIFEEMVALSRELGFRVRVLAEG"
     gene            complement(2918..3232)
                     /locus_tag="AC16YU_01710"
     CDS             complement(2918..3232)
                     /locus_tag="AC16YU_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063965.1"
                     /translation="MRHPGVKRIRIGLTAAAAGRTEEAREVARKIESKPRLWDSYGLA
                     QIYKALGDADRVFYWLEVGCEQRHPCIKWFQRPGNFPEFGADPRFAELARRFGLPADG
                     SG"
     gene            3224..3508
                     /locus_tag="AC16YU_01715"
     CDS             3224..3508
                     /locus_tag="AC16YU_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063966.1"
                     /translation="MSHHYEDLVLIFTDVELNDEPAIVSGEPVLTLREAGVVSPNEPL
                     LQHFHSAGTVIQGTGIFESWVGRRAVSRGWLDTDPETMLPIHAWGPTILQ"
     gene            complement(3642..6635)
                     /locus_tag="AC16YU_01720"
     CDS             complement(3642..6635)
                     /locus_tag="AC16YU_01720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR6063967.1"
                     /translation="MQSARRQEHGVSSFAIHGAAVLLLLLIGLPRVGLAQPDSGDRMS
                     RTLQVRLAEGQVPGAFISQVRREYATLIESVRPLFSLAPEQLAALRAQGEARTGRRLP
                     DLNLWIRITLEDVSQVEPMIETLGRMDAVDVVERAPVPLPPPQITPDFEASQGYLDAA
                     PGGIDARFAWTLPGGTGSGITVIDVEYDWNQTHEDLSKVHGLALLLNGGDANDPPFPP
                     DHGTAVLGELIADDNGLGVTGIVHDAGIGLAPANTQDLGYNVANAITLAQAATGPGDL
                     ILIEQQICVCDIDCPNDGTQSGSGPSEWEQAVFDAISNATAAGRIVVEAAGNGSVNLD
                     MDSCDDKFDRTNRDSGAIIVGAGQPPATGNDRERYNFSSYGSRLDLQGWGGGVATTGY
                     GFGYMDPDDPTNEDRWYDDGFGGTSSASPIVTAAVAALQGIALANGDPLFTADEVRDL
                     LIATGSPQLGNTAQHIGPRPNLAAAIITQFPELFPPEITGISPSSQTVQYSDNISAVT
                     ITAEDIGSDVLTINRAGLPSDLSILPANDEDACTDTMYGTECEWTLEGQMLEASQVFL
                     LTVTVTDTDGNTDDNTDVGDPELEITVEEEDVTVTFDPANPVGVLVDSEGGDSGPFTL
                     TTYVTEFDDPEGVAPNLAGDITLADVGMNLVALVGGGVIAGTCTPEAGPISGYEPLQV
                     DCSFDNVAVNAYAVDVTVNGGYYEGSGEDVLVIFDPSLGFTTGGGWFYWPETTDRTSF
                     GFTVQYNGGSIKGKGKGVQAQEPKAQGGLLVIRHLGDGTSHRLKSNAIAALALDPDNA
                     WATFLGKATYRAPGWEESEGDYDFMAYVEDHDTPGSGADEFWLQTLDPSDAVLPALSL
                     DEPMADHAVVIEAGNIVVPQAGGNGKNKEAPGQTAAKAGEAEVEVTPDVGIGLPESFD
                     ISGNYPNPFNPSTSIRVELPDAAHVSFVVYDATGRAVDHVADREYPAGIHTLSWSARD
                     LPSGVYFYRIVAGPLQVTRPMVLLR"
     gene            7071..>7292
                     /locus_tag="AC16YU_01725"
     CDS             7071..>7292
                     /locus_tag="AC16YU_01725"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR6063968.1"
                     /translation="MRRLRYQVAASLDGYIAGPNGEYDWIPDEPTYDFAKFYSGIDTV
                     LMGRRTFEPLSDGGAGFLSGMEIVVFSTTL"
BASE COUNT         1157 a         2514 c         2409 g         1212 t
ORIGIN      
        1 cttcctcgcg tgcgtcctct tcacttgggt gatcctctcc cgcctgcggc tcggtcggca
       61 catctacgcg gtgggcggca acgaggaggc cgcgcgcctc tcgggcgtgc cggtccggcg
      121 tacgctcatt ctcgcttacg ccctgtcggg cctcttctcg gccgtcgccg gcatctgcca
      181 ggccgcgcag gagacgcagg gcgatcccga gaccggcatg gcttacgagc tcgacgccat
      241 cgcgatggtg gtcatcgggg gcacgacgct tgccggcggg aagggttcgg tcatcctgac
      301 gctcatcggc gtcctcacga tcggctacct gcagaagatc ctctcgctga atgccgcacc
      361 cgaggccacg cgcctcatgc tgacgggcgc catcatcatc atcgccgtgc tgttccagcg
      421 cggccgttag cccatgagat atctgcccgt cctcttcgtt ctcctcgcag cctgcggctc
      481 tcccggcccg cgcggccacg ccggcaccgc ggaggatccc tgggtgatcg gcatgagcca
      541 gtgcaacctg ggcgagccct ggcgggtgca gatggacgcc gacgtgcgcg aggccgcggc
      601 ggcgcacgac aacctgcggg tcgtcttcaa ggacgcccag aacaatgccc tcacgcagcg
      661 cgcccaggtc gaggagttcg tggagcaggg cgtggacctc ctcatcatca gcccgaagga
      721 ggccgcgccg ctcacggcgc ccgtcgcgga ggcctaccgg cgcggcatcc ccgtcatcgt
      781 cctcgaccgc cgggtccagg gcgacgcgta cacggtcttc atcggggcgg acaacgtccg
      841 gatcgggcgc gaggcgggcc ggtggattcg cgagacgctc gtcggcgagg gccggatcgt
      901 ggagctgaag ggcctgatga cctcgacgcc cgggcaggac cggcactcgg gattcctgga
      961 gggcctcgac ctggaatcga atcccgggct cgagatcgtg ttcgagggag agatgcagtg
     1021 gctcgagccg aatgcgaggc gcgagatgga gtcggcgctc gccaccaacg accggatcga
     1081 cgctgtctac gcccacaacg accccggcgc gcacggcgca tggctcgccg cacgggccgc
     1141 cggccgcgag gaccagatgc tcttcgtggg catcgacgcc ctcccccacg agggacggca
     1201 gtacgtcgcg cagggcatca tggacgccac cttcctctac cccaccggcg gggccgaggc
     1261 catcgacgcc gcgctgacgc tgctcggcgg cggcacggtc ccgaaggaga tcgtgctagg
     1321 cacccggctc ttcacgggcg agaacctgga agacggcggc gaggccatcg aataaccgga
     1381 tcggagcatg aaacagcgga tcgccctctt ctggcccggc gacgggcgcg acatcccgaa
     1441 cgagctcgcc gtgccgagcg ccaccgaggc gacgcgccag atggaggccg ccctccgccg
     1501 cctggaccgc gagccctacc ggatcgaggg ctggctcacg aagccgcacc acgcgatcga
     1561 gaggctcggg cccgtccgcg acccgatgat cggtatctgc gtgcactggt tctacgggcc
     1621 gcacacgacg gacggcgtgg tgggcaagga caacccgcta cttctcgcca gcaatttctc
     1681 gggccagtgg ccgggcctcg tcgggctcct gaacacgggc gcctgcctgg cgagcctgaa
     1741 ccggccgttc tcccgcgtct ggacggacgc cgaggactgg acccgcgacg acctcttcat
     1801 gtcgcgactg gaacagtggt gcgaggaagg ctcgatcgtc tacgacgtgg acgcgctgca
     1861 cttcccgacc tcggtcccgc ccggggcggc cgagctggcc tcgaaggtcg cgcgcggatt
     1921 ccgcgaccgg cgggcgctca tcctgatgct cggcgacacg tcgatgggca tgatcaacgg
     1981 gtacttcgga ccgcgtctgc tcgccccgca cggcttcgcc gagcacaaga tcgaccaggc
     2041 ctggatcgtg gaccgcggca gccggatcgc ggacgggcgg atcgacgacg ccttccgctt
     2101 cgtgcaggac aggggcgtga ctttccactg gcgcgaaacc ggcgcggagg acttcaccga
     2161 ggactcgacc cgcgagcagc ttcgcgacta tctcgccgtc ctggacatgg cggacgagta
     2221 ccgggccgac tgcatcggct ggcagtacca gctggggctc atcccgctcc ggccgccgtc
     2281 ggacttcgcc gagggcctcc tgaactcgac ctgccggccc gaatcgaacg gcgacacgat
     2341 cgtgacggcc accgaggccg accagggcaa cgccgtcccg atggagatga tgaagcggct
     2401 cctgaaggcg aagggcctgc accagtcggt cgccttccac gacgtgcgct ggggaggcga
     2461 gcacgagggg cgcttcatct gggtgctcct gaactcgggc tcgaccggcg cctttccctt
     2521 caaccacgac ccggacacgc tcgagggcgc gcacagctac cggcaggccg ccatctactt
     2581 cccgttcgcg ggcggcaccc tgacgggcga gtcgctgccc ggccgggtga cgtggtcccg
     2641 cgcgtacatc cggaacgacc agatgtggat ggacatcgga cgcggcgagg tagtctccct
     2701 cccgccgaag aagcgcgacg cctggtggaa cggcaccaac cgccagtggc ccttcatggc
     2761 ggccgacctg ggcatcgggc gcgacacgct gatggcccac tacatgtcca accacatcgc
     2821 ggtcgcgtat ggggacatct tcgaggaaat ggtggcgctg tcgcgggagc tggggttcag
     2881 ggtgagggtg ctggcggagg ggtgacgggg gccgccgtca accggaaccg tcagcgggca
     2941 ggccgaaccg cctggccagc tccgcgaacc gcggatcggc gccgaactcc gggaagttcc
     3001 ccggccgctg gaaccacttt atgcaggggt ggcgctgctc gcagcccact tccagccagt
     3061 agaagaccct gtccgcatcg ccgagagcct tgtagatctg ggccagccca tagctgtccc
     3121 acagcctggg cttgctctcg atcttccggg ccacctccct ggcctcttcc gtcctgcctg
     3181 ccgcggcagc ggtaagccct atgcggatcc gtttcactcc gggatgtctc atcactacga
     3241 ggatctggtg ctgatcttca ccgacgtgga gctcaacgac gaaccggcca tcgtgtccgg
     3301 agaacccgta ctcacgcttc gcgaggctgg cgtggtatca ccgaacgaac cgcttctgca
     3361 gcactttcat tctgcgggta cggtcatcca gggcacgggc atcttcgaat cctgggtggg
     3421 gagacgagcc gtctcccgcg gatggctgga cacggatccg gagaccatgc ttcccattca
     3481 tgcctggggc ccgaccatcc ttcaataggc cccgtccgcc tctcccttgg tcgccggcgc
     3541 gtcagacgga cataccgtct ccgcggccgg atcgcacgat gacaggggcc ccgccggatg
     3601 cggcgaggcc cctgtttctg cccggagacg agcggtatcg ctcagcggag cagcaccatg
     3661 ggccgcgtca cctggagcgg gccggccacg atgcggtaga agtacacgcc gctcggcagg
     3721 tcccgcgcgc tccacgacag cgtgtggatg ccggccggat attccctgtc ggccacgtgg
     3781 tccacggccc ggccggtcgc gtcgtacacg acgaacgaca cgtgcgcggc gtcgggcagc
     3841 tcgacccgga tcgacgtgga cgggttgaac ggattggggt agttgcccga gatatcgaac
     3901 gactcgggaa gccctatgcc gacatcgggt gtgacctcga cctcggcttc cccggccttg
     3961 gcggccgtct ggcccggcgc ctccttgttc ttcccgttcc cgcccgcctg cggcaccacg
     4021 atgttgccgg cctcgatgac cacggcgtgg tccgccatcg gctcgtccag ggagagcgcg
     4081 ggcagcacgg cgtccgacgg atcgagcgtc tgcagccaga actcgtccgc cccggacccc
     4141 ggggtgtcat ggtcttcgac gtaggccatg aagtcgtagt ctccctcgga ctcctcccag
     4201 cctggcgccc ggtaggtcgc cttgcccagg aaggtcgccc aggcgttgtc cgggtccagc
     4261 gcgagggcgg cgatggcgtt gctcttcagg cgatgggacg tcccgtcgcc caggtgccgg
     4321 atcacgagca ggccaccctg cgccttcggt tcctgggcct gcacgccctt gcccttgccc
     4381 ttgatgctgc ctccgttgta ctgcaccgtg aagccgaaac tcgtgcggtc cgtcgtttcc
     4441 ggccagtaga accagccgcc gccggtcgtg aagcccaggg acgggtcgaa gatcacgagg
     4501 acgtcttcgc ccgagccttc gtagtagccc ccgttcacgg tgacgtccac cgcgtaggcg
     4561 ttcacggcga cgttgtcgaa tgagcagtcc acctgcagcg gctcgtagcc ggatatcggt
     4621 ccggcctcgg gagtgcaggt gcccgcgatc acgccacctc cgacaagcgc cacgaggttc
     4681 atgcccacgt ccgcgagcgt gatgtccccc gcgagattcg gggccacacc ctccgggtcg
     4741 tcgaactcgg tcacgtaggt cgtgagcgtg aacggacccg aatcgccgcc ctcgctgtcg
     4801 accagcacgc cgaccgggtt ggccgggtcg aacgtgaccg tcacgtcctc ctcctcgacc
     4861 gtgatctcaa gctccggatc accgacgtcc gtgttgtcgt cggtgttgcc gtccgtgtcc
     4921 gtcacggtga cggtcagcag gaagacctgg gaagcctcca gcatctggcc ctccagcgtc
     4981 cattcgcact cggtgccgta catcgtgtcc gtgcaggcat cctcgtcatt cgccggaaga
     5041 atcgagaggt cggacggcag cccggcccga ttgatcgtga gaacgtccga tccgatgtct
     5101 tccgccgtga tggtgacggc ggagatgttg tcactgtact ggacggtttg cgacgacggg
     5161 ctgatcccgg tgatttcggg cgggaacagt tcggggaact gcgtgatgat cgcagccgcc
     5221 aggttcgggc gcggaccgat gtgctgggcg gtgttgccca gttgcgggct tccggtcgcg
     5281 atcaggagat cacgcacctc gtctgcggtg aacagcggat cgccgttggc cagcgcgatc
     5341 ccctgaagtg cggccacggc ggcggtcacg atgggggaag cgctggacgt acccccgaag
     5401 ccgtcatcgt accagcgatc ctcattggtc ggatcgtccg gatccatgta tccgaaaccg
     5461 tatccggtag tcgccacgcc tcccccccat ccctgaagat caagacggct tccatacgag
     5521 gagaagttgt accgttcgcg gtcgttcccg gtggcggggg gctgcccggc gcccacgatg
     5581 atcgcgcccg agtcacggtt cgtgcgatcg aatttgtcgt cgcagctgtc catgtcgagg
     5641 tttaccgagc cgttgccagc ggcctcgacg acgatgcgac cggccgcggt cgcattggaa
     5701 atggcatcga aaacggcctg ctcccattcc gaaggtccac tccccgattg cgtcccgtcg
     5761 ttcggacaat cgatgtcgca gacgcagatc tgctgctcga tcaggatgag atcgccgggg
     5821 cccgtggccg cctgggcgag agtgatggca tttgcgacgt tgtagccgag atcctgcgta
     5881 ttcgcggggg ccagcccgat gccggcgtcg tgaacgatgc cggtgacgcc aaggccgttg
     5941 tcgtcggcga tgagctcacc gagcaccgcc gtgccgtgat cgggaggaaa gggcgggtcg
     6001 ttggcgtctc cgccgttcag caggagcgcg agtccgtgca ccttgcttag gtcttcgtgg
     6061 gtctggttcc agtcgtattc cacgtcgatc acggtgatgc ccgaccccgt accgcccggc
     6121 aaggtccagg caaaccgtgc gtcgattccg ccgggcgcgg catccaggta cccctggctg
     6181 gcctcgaagt ccggggtgat ctgggggggc ggcaggggga cgggcgcccg ttccacgacg
     6241 tcgacggcgt ccatccgccc aagcgtctcg atcatgggtt ctacctgcga gacgtcctcg
     6301 agcgtgatgc gaatccagag gttcaggtcc ggcaatcgtc gtccggtacg agcctcgccc
     6361 tgcgcgcgga gcgcagccag ctgttccggc gcgagcgaaa acagcggacg cacgctttcg
     6421 atgagggtgg cgtactcccg cctcacctgc gagatgaacg ctcccgggac ctggccttcg
     6481 gcgagtcgca cctggagcgt acgggacatc cggtcgcccg agtccggctg cgccaacccg
     6541 acccgtggca agccgatcaa gaggagaaga agcaccgccg cgccatggat ggcgaacgac
     6601 gacacgccat gttcttgccg tctggctgac tgcatgatac acctccatca gtgttgattt
     6661 ccccgacggg tgtggacatg tcccgagcgc cccgtggcag ctggcgcccg gcacgtcgac
     6721 gagaggtgca cgggggggta gtattcctct ccggagacgc ttgctgatac tgcccacggg
     6781 ccacgatcgg cacgcccccg ggcgacgctt gcaccatgcg cccaacctgc cgaactgcaa
     6841 atccacggca aggctttcgt gcaagcagcc tcgggcaacc gccgaacccg gctgccttca
     6901 tctcacagtc acgcctccgc gattgaatta atcggaaaca ccgcgggcat tgtcaatact
     6961 ttacgcggac tcccggcatg ggcaggcact gcctgtgccg ctcccgcagg gtatcccgaa
     7021 tgcgtgccga ttcggatgcc gttccggcac gagccccgcc ccctaccccc atgcgccgcc
     7081 tccgctacca ggtcgccgcc agcctcgacg gctacatcgc cggcccgaac ggcgaatacg
     7141 actggatccc cgacgaaccg acctacgatt tcgcgaagtt ctactccggg atcgacaccg
     7201 tgctcatggg tcgcaggacg ttcgagcccc tctctgacgg cggcgccgga ttcctgtcgg
     7261 gcatggagat cgtcgtgttc tcgaccaccc tc
//
LOCUS       DBNHQZ010000070         7782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3406755, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000070 DBNHQZ010000000
VERSION     DBNHQZ010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3406755"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16YU_01730"
     CDS             <1..283
                     /locus_tag="AC16YU_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6063969.1"
                     /translation="LSVSEIAGKGFTDRGSFHGHVLDPRTGRPVTGALLAAVVGPSAA
                     GTDALATALLVLGADARTATAFRDARHRWLVALRGERGPEFVHMGLPPG"
     gene            377..1633
                     /locus_tag="AC16YU_01735"
     CDS             377..1633
                     /locus_tag="AC16YU_01735"
                     /inference="COORDINATES: protein motif:HMM:NF046806.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6063970.1"
                     /translation="MDRRRFLQRSSLGTLIGLAGAGDVLHGARAGARRRDALSDEQLA
                     CAVVGFGEWGRQVALEVEGVPELRLAAVCDEYPVMLRRAQRAHPDAARHASVDAVLDD
                     PAIPAVLVATPTHRHLEVVRKALAAGKHVYCEAPMAASIEDARAIAEAAAGSPGQIFQ
                     VGLHLRTEPQYRSVFQFIRSGALGRAAMGRAQWHAKESWRRASPDPERQRAQNWRLDP
                     EISLGLVGETGMQQMDASNWVFGDYPVAVTGFGSTLYWTDGRDLPDTVQAVFEYPGGQ
                     RMLWDGTLVSSFDSAHEVFYGSDSTIMLRDQKAWMFKEVDAPMLGWEVYARKDKFYRE
                     TGIALIANATKLEAQGLDPAADDPNRKSPLWHALKAFADNYFFGPYPPVADHVLGYAN
                     TVLAVRARQAVAEQRRVVIDPAEYVL"
     gene            complement(1640..2422)
                     /locus_tag="AC16YU_01740"
     CDS             complement(1640..2422)
                     /locus_tag="AC16YU_01740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6063971.1"
                     /translation="MHLVDTHAHLYLDRFDEDREDVIRRAAEAGVTGIVLPAIDVPSI
                     HAALALAARHRGIHVMAALHPSETKDATDADWLEVESLSREPAVVAIGETGLDYHWDR
                     SFDDRQREFLRRHIALAEERDLPLVFHDRKASDDLVRIVDEARAASERPERIRGVFHC
                     FGGPAELAFRVLGLGFHVGLGGTLTFRNSGVADAVREVPLERIVLETDAPFLAPAPNR
                     GKRNEPAWVRLVADRLAEVRAEPVEHVAAVTTANARRLFGLP"
     gene            2593..5094
                     /locus_tag="AC16YU_01745"
     CDS             2593..5094
                     /locus_tag="AC16YU_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR6063972.1"
                     /translation="MDRFDRSASVLLVLVVLLALAPIGRLAYDWWTIRAYLAGAQAPP
                     MESAEGFDWVEWTERDGHIEATWVFPRGPGADAGIRTGDRFFMLEYQQFFDVSDLRNA
                     VRSMGPGETRDYLLERGGEYVAAEVRLARYPTFLYPRSSSLWYFALWAFAIGAFVHLI
                     ALFVARPLARRSREARAEFLLILVSSVWVFGNLLRLALLELIGPPAAGTTYDHLLQGI
                     TFAGLVGWIAFPAFLLHEVTSDVLGRDRSLPIRVLLFLPAAVLALSLAAVSVRGHLGP
                     VTPEGLLVPVLFYTSCYIAAAAVVVQASYRFRPLRAHAVFGGWGQAGSLVILLVALLA
                     ALSVQEVVPLLDLAGDVAAGWIVVAAQLLAVAPVTMFSYGTLRHGKIEEVVSRALVYL
                     TVLGVIFFLFVAFTSLLDPTLQALGGSRHVVEALFVVALLMVADRLGRRLRGVATSFF
                     RTDREKGRQLLSHFQEQMLELVDPDVLCRQTVETLAEALQARSAVLHLLVPGPSLRWV
                     SASWNPEPPFLTERIFRLVWPHFQDDPRIWVRNDELDERGLPTDAEYLLTERGAAVAV
                     PIRGEGVSIGLLVLGTKRKRGSVYNLEDLDNLRSLAGQLALAADRIARVEREKDLARE
                     SSEAQLVALRAQINPHFLFNALNTILSHIGERPDSAEEAVERLAAMFRHTLQTDKRPF
                     VPLESELELIEHYLRVEQARFGPGLEVEIRIDPAVRDHPVPAFIVQTLVENAVKHGIE
                     RKRGGGRLAIVAEASDAGFARISVSDTGVGIQQLFVPDGYADRSFFGIGLSNAASRLA
                     LLYGRDDLLELRSDPETGTVATILLPPRQLTETGT"
     gene            5120..5956
                     /locus_tag="AC16YU_01750"
     CDS             5120..5956
                     /locus_tag="AC16YU_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR6063973.1"
                     /translation="MIHVFIVDDEAPARARLRKLLEPFADDGRLEVAGEAKDGVEAVE
                     WLSANPADVVFLDIRMPGLDGFEVLERLPPDRRPIVVFTTAYDEYAIRAFDSNAVDYL
                     LKPFGKERLEEAVARVERLRGSESSIRDREDRLARLLDWFDAQAESKHAVPSGGYVQH
                     LSIPYRDRILVTPVEQIVSAEITEGITRLNVVDDTPVPSGPPRPRIRQHIVGHTLDQL
                     AAMLDPEQFMRVHRSAIVQFNHIRELIPWFSGRYKLILTGNHEVIASRERSKVLKEKL
                     NL"
     gene            complement(5912..7744)
                     /locus_tag="AC16YU_01755"
     CDS             complement(5912..7744)
                     /locus_tag="AC16YU_01755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10-like protein"
                     /protein_id="HIR6063974.1"
                     /translation="MTRLFLLLLVAPWPARPARAQDAPRHEFRGAWIATVANLDWPSS
                     GRLAPAEQRAELVRMLDRLGDLGVNAVFFQVRSECDAMYDSAVEPWSRFLTGEQGRPP
                     EPRWDPLTFAVDEAHRRGMALHAWLNPLRCVRDVARDRLAASHPARRRPDWMLTIGSD
                     AEPGRAMKILNPGLEAVREYVTGIVLDVARRYDVDGIHFDDYFYPYPPLGVGTEDLAD
                     WQSDPRGLSLADWRRDNVNRLVRGVSRALARERPDVVFGISPFGIWRSGVPRGVVGTS
                     AADEIYADARAWLENGWIDYLAPQLYWDFGGGQDFGALVRWWASVADGRHVYPGLAAY
                     KADAATHAGELLAPGVVPDQIRYARGVEGVHGHAVYRASNLTRYGAAGLAREIGSELY
                     GRPALLPPMRHLLGAAPEPVRNVSAREEAAVGIRVEWDLPPFSFVRPRRFALFRVQGD
                     APVDPARVAADVGNLVAVVYAPRRHYSDPVAPSGGPYHYVVVALSANAAESVPSEPVS
                     VTVTGRPAAAPAHPVADVPAAAEQASGEVLRVTVTDRMGRELARFEPPSIPAGAPGDL
                     ARRVPGPPGLYFVIVETAGRRAVHPVLRRWSQVQLLLQHLGALP"
BASE COUNT         1104 a         2855 c         2687 g         1136 t
ORIGIN      
        1 gctctccgtc tccgagattg ccgggaaggg gttcaccgac cggggctcct tccacggcca
       61 cgtactcgac ccgcgcaccg ggcgcccggt gaccggcgcg ctgctcgctg ccgtcgtcgg
      121 accgtcggcg gccgggacgg acgccctcgc gaccgcgctc ctggtgctgg gcgccgatgc
      181 ccgcaccgcg accgcgttcc gcgacgcgcg ccaccggtgg ctggtcgccc tgcgcgggga
      241 gcgcggcccg gagttcgtgc acatggggct cccgcccgga taggccccgc ccgggtgccc
      301 cggcgcacgg atgcgcccct cgcaggatgc gccgtcagac cctaacttgc cgctcgctcg
      361 cgaaccggac cggaccatgg atcgtcgtcg attcctccag agaagctcgc tcggaaccct
      421 catcggcctt gccggggcgg gagacgtgct gcacggggcc cgcgccgggg cccgtcggcg
      481 ggatgcgctc tccgacgagc agctcgcctg cgcggtggtc ggcttcggcg agtggggccg
      541 gcaggtcgcc ctcgaggtgg agggcgtccc cgagctccgc ctggcggccg tctgcgacga
      601 gtacccggtg atgctccgac gggcgcagcg ggcccacccg gacgcggccc gccacgcctc
      661 ggtcgacgcc gtgctggacg accccgcgat ccccgcggtc ctggtcgcaa cgccgacgca
      721 ccgccacctg gaggtcgtcc ggaaggcgct cgcggccggc aagcacgtct actgcgaggc
      781 gccgatggcc gcgtcgatcg aggacgcccg ggccatcgcc gaggcggccg ccggctcccc
      841 ggggcagatc ttccaggtgg gcctccacct ccggacggag ccccagtacc gttcggtctt
      901 ccagttcatc cgcagcggtg ccctcgggcg cgccgcgatg ggccgcgcgc agtggcacgc
      961 caaggagagc tggcggcgcg cctcgcccga ccccgagcgg cagcgggcgc agaactggcg
     1021 tctggatccc gagatcagcc tgggactggt cggggaaacg ggcatgcagc agatggacgc
     1081 gtccaactgg gtcttcgggg actacccggt cgcggtcacg ggattcggct ccacgctgta
     1141 ctggacggac gggcgggacc tgcccgacac ggtccaggcc gtattcgagt atcccggcgg
     1201 gcagcgcatg ctgtgggacg gcaccctcgt gagctccttc gactccgccc acgaggtgtt
     1261 ctacggctcg gacagcacga tcatgctgcg cgaccagaag gcctggatgt tcaaggaggt
     1321 cgacgccccg atgctgggct gggaggtcta cgcgcggaag gacaagttct accgcgagac
     1381 gggcatcgcg ctcatcgcga acgcgacgaa gctcgaggcc cagggcctgg acccggcggc
     1441 cgacgacccc aaccggaagt ccccgctctg gcacgccctg aaggcgttcg ccgacaacta
     1501 cttcttcggc ccctacccgc ccgtggccga ccacgtgctg ggttacgcga acaccgtgct
     1561 ggcggtcagg gccaggcagg ccgtggccga gcagcgccgc gtcgtgatcg acccggccga
     1621 gtacgtcctg tagacgggcc tacggcagcc cgaacaggcg ccgggcgttc gccgtcgtga
     1681 cggccgccac gtgctccacg ggctcggcgc gcacttcggc gagacgatcc gccacgaggc
     1741 ggacccaggc cggctcgttc cgcttgcccc ggttgggggc gggggccagg aacggcgcgt
     1801 cggtctcgag cacgatccgc tcgagcggca cctcgcgcac ggcgtcggcc acgccggaat
     1861 tgcggaaggt aagcgtacct ccgagcccca cgtggaaccc gagaccgagc acgcggaagg
     1921 ccagctccgc tggcccgccg aagcagtgga agacgccccg gatccgctcc ggcctttcgg
     1981 acgccgcgcg ggcctcatcc acgatccgga ccaggtcgtc ggaggccttc cggtcgtgga
     2041 agaccagcgg aaggtctctc tcctcggcca gcgcgatgtg tcgccggagg aactcgcgct
     2101 ggcgatcgtc gaacgagcgg tcccagtggt agtcgaggcc ggtctcgccg atcgccacga
     2161 ccgcgggctc gcgcgagagg ctctccacct ccagccagtc ggcatccgtc gcgtccttcg
     2221 tctcggacgg gtgcagggcc gccatcacgt ggatgccccg gtgccgggcc gccagagcga
     2281 gcgccgcgtg gatggacggc acgtcgatgg ccggcaggac gatgcccgtc acgcccgcct
     2341 cggcggcccg gcggatgacg tcctcccggt cctcgtcgaa ccgatccagg tacaggtggg
     2401 cgtgggtgtc tacgagatgc atggacggcg gagtgccccg aatcggatcg ttcggatggc
     2461 cggagcgacc ggtcgcggcc cgcggtggac tgcccgcggc gctccatgct accttccgac
     2521 ggagccaccc aacggccgga acgtaccagc gttccgaagg acgccgttcg tccggagacc
     2581 cactttcacc cgatggaccg tttcgaccgt tccgcctccg tcctgctcgt cctggtcgta
     2641 ctgttggcgc tcgcgccgat cggacggctg gcctacgact ggtggaccat ccgggcgtac
     2701 ctggccgggg cgcaggcgcc gcccatggag tccgccgagg ggttcgactg ggtggaatgg
     2761 accgagcggg acggccacat cgaggcgacc tgggtctttc cgcgcggccc gggcgcggac
     2821 gcaggcatcc ggaccggcga ccgcttcttc atgctcgagt accagcagtt cttcgatgtc
     2881 tcggacctgc gcaacgccgt gcggtccatg gggccggggg aaaccaggga ctacctgctc
     2941 gagcgcggcg gcgaatacgt cgcggccgag gtccggctcg cgcggtaccc gacgttcctc
     3001 tacccgaggt cgtccagcct gtggtatttc gccctgtggg ccttcgcgat cggggcgttc
     3061 gtgcacctga tcgcgctgtt cgtcgcacgt ccgctcgccc ggcgcagccg cgaggcgcgg
     3121 gccgaattcc tgctgatcct ggtctcttcg gtgtgggttt tcggcaacct gctgcggctg
     3181 gccctgctcg agctcatcgg tccccccgcg gccggtacga cctacgacca cctgctgcag
     3241 gggatcacgt tcgcgggcct ggtgggctgg atcgcctttc ccgccttcct gcttcacgag
     3301 gtcacgagcg acgtcctggg ccgcgatcgt tcgctgccga tccgcgtcct gctgttcctg
     3361 ccggccgcgg tcctcgccct ctccctcgcg gccgtctccg tccgggggca cctggggccc
     3421 gtcacccccg agggcctgct cgttcccgtg ctgttctaca cgtcgtgcta catcgccgcg
     3481 gcggccgtcg tcgtgcaggc ttcgtaccgc ttccggcctt tgcgcgccca tgcggtgttc
     3541 ggcggctggg gccaggccgg aagcctcgtc atcctcctgg tcgcgctgct ggctgccctg
     3601 tcggtgcagg aagtggtgcc gctgctggac ctcgccggcg acgtggccgc cggctggatc
     3661 gtcgtcgccg cccagctgct cgccgtcgcc ccggtgacga tgttctcgta cggcacgctc
     3721 cggcacggca agatcgagga ggtcgtgtcg cgggcgctgg tttacctgac cgtcctgggc
     3781 gtcatcttct tcctgttcgt ggctttcact tcgctcctcg acccgaccct gcaggccctc
     3841 ggaggttctc ggcatgtcgt ggaggccctg ttcgtcgtgg ccctgctcat ggtggcggac
     3901 cgactgggac ggcgcctgcg gggcgtggcc acgtcgttct tccggacgga ccgcgagaag
     3961 ggccggcagc tgctcagcca cttccaggag cagatgctcg agctcgtgga cccggacgtg
     4021 ctctgccgcc agaccgtcga gacgctggcg gaggccctcc aggcccgatc cgccgtcctc
     4081 cacctcctcg tgccgggccc gagtctccgc tgggtgtccg cctcgtggaa cccggagccg
     4141 cccttcctga ccgagcgcat cttccgcctg gtgtggcctc atttccagga cgatccacgc
     4201 atctgggtgc gcaacgacga gctggacgag cgcggcctcc ccacggacgc cgaatacctg
     4261 ctcaccgagc ggggggccgc cgtggccgtt ccgatccggg gcgaaggcgt gtcgataggt
     4321 ctcctggtgc tcggcacgaa gcgcaagcgg ggctccgtct acaacctgga agacctggac
     4381 aacctgcgct cgctcgccgg gcagctcgcc ctcgcggccg accgtatcgc gcgtgtggag
     4441 cgggagaagg atctcgcccg cgaatcctcc gaggcccagc tcgtcgccct gcgggcgcag
     4501 atcaatccgc acttcctgtt caacgcgctg aacaccattc tctcgcacat cggcgagcgg
     4561 cccgactccg ccgaggaggc cgtcgagcgg ctggcggcga tgttccggca cacgctccag
     4621 acggacaagc ggcccttcgt cccgctcgag agcgagctgg agctcatcga gcactacctg
     4681 cgcgtggagc aggcccgctt cggtcccggg ctcgaggtcg agatccggat cgacccggcc
     4741 gtgcgcgacc atccggtgcc cgcattcatc gtccagaccc tggtcgagaa cgcggtcaag
     4801 cacggcatag agcgcaagcg gggcggcggt cgccttgcaa tcgttgcgga agcgtccgat
     4861 gccggcttcg cccggatctc ggtctccgac accggggtcg gcatacagca gctcttcgtt
     4921 ccggacgggt acgccgatcg cagcttcttc ggcatcggcc tctcgaacgc ggcgagccgc
     4981 ctcgcgctgc tctacggccg ggacgacctc ctcgagctgc ggagcgaccc ggagaccggg
     5041 accgtcgcca ccatcctgct tcctccacgg cagctcaccg agaccggcac ctgaccccac
     5101 gacccaacct ccaagcgcca tgatccacgt attcatcgtc gacgacgagg cgcctgcccg
     5161 ggcccgtctc cgcaagctcc tggaaccgtt tgccgacgac gggcggctcg aagtggccgg
     5221 cgaagcgaag gacggcgtcg aggccgtcga gtggctctcc gcgaacccgg cagacgtcgt
     5281 gttcctggac atccgcatgc ccggcctgga cggcttcgag gtcctcgaaa ggctcccccc
     5341 cgaccgccgg cccatcgtcg tgttcacgac ggcgtacgac gagtacgcca tccgggcgtt
     5401 cgattccaat gccgtcgact acctgctgaa gcccttcgga aaggagcgcc tcgaggaggc
     5461 ggtcgcgcgc gtggaacgcc tccgcggcag cgaaagctcg atccgtgacc gagaggaccg
     5521 tcttgcacgg ctcctggact ggttcgacgc ccaggccgaa tcgaagcacg ccgtgccttc
     5581 gggcggctac gtgcagcacc tgtccattcc gtaccgcgac cgcatcctgg tgacgcccgt
     5641 cgagcagatc gtctcggcgg agatcaccga gggcatcacg cgtctgaacg tggtggacga
     5701 cacgccggta ccgtcgggac cgccccgtcc ccggatccgg cagcacatcg tcggccacac
     5761 cctggatcag ctcgccgcga tgctcgaccc cgagcagttc atgcgcgtgc atcggtctgc
     5821 catcgtgcag ttcaaccaca tccgggaact catcccctgg ttctcgggcc ggtacaagct
     5881 gatcctgacc ggcaaccacg aggtgatcgc ctcacgggag cgctccaagg tgctgaagga
     5941 gaagctgaac ctgtgaccac cggcgaagca cgggatgcac ggcccgacgc cctgcggtct
     6001 cgacgatgac gaagtacagg cccggaggcc cgggcacgcg ccgcgcgagg tccccgggag
     6061 cccccgcggg gatcgacggc ggctcgaagc gggccagctc gcgacccatc cggtcggtca
     6121 ccgtgacgcg caggacctcc ccggacgcct gctccgcggc ggccggaacg tcggcgaccg
     6181 ggtgcgcggg cgcggcggcc ggccggccgg tcaccgtgac ggacaccggc tccgacggca
     6241 cgctctcggc cgcgttcgcc gaaagggcaa ccacgacgta gtgatagggc ccgccggacg
     6301 gcgccaccgg gtcgctgtaa tgacggcgcg gcgcgtagac caccgcgacg aggttgccca
     6361 cgtcggccgc cacgcgagcc gggtcgacgg gcgcgtcccc ctgaacgcgg aacagcgcga
     6421 accggcgggg ccggacgaag gagaacggcg gcaggtccca ctcgacccgg atgccgacgg
     6481 ccgcctcctc ccgggcgctc acgttgcgga ccggctcggg agccgccccg agcaggtgcc
     6541 gcatcggcgg caggagcgca ggacgaccgt acagctccga tccgatctcc cgggcgaggc
     6601 cggccgcccc gtagcgcgtc aggttcgacg cccggtacac ggcgtggcca tgaacgcctt
     6661 cgacgccgcg ggcataccgg atctggtccg gtaccacgcc gggagcgagc agctccccag
     6721 cgtgcgtcgc ggcatccgcc ttgtacgccg cgagacccgg gtacacgtgc ctgccgtcgg
     6781 cgaccgaagc ccaccatcgc acgagcgcgc cgaagtcctg gccgcccccg aaatcccagt
     6841 agagctgcgg agccaggtag tcgatccagc cgttctcgag ccacgcgcgg gcatcggcgt
     6901 agatctcgtc ggccgcggac gtccccacga cgcccctcgg cacgccgctc cgccagatgc
     6961 cgaagggact gatgccgaag accacgtccg gccgctcgcg cgcgagtgcc ctcgagacgc
     7021 cccggaccag ccggttcacg ttgtcgcgcc gccagtccgc aagcgagagc ccgcgcggat
     7081 cgctctgcca gtcggccagg tcctccgtgc ccacgccgag cggcggatac ggatagaagt
     7141 agtcgtcgaa gtggatgccg tccacgtcat accggcgggc cacgtccagg acgatccccg
     7201 tgacgtactc ccgcacggcc tcgagcccgg ggttcaggat cttcatggcc cggcccggct
     7261 cggcgtcgct cccgatcgtc agcatccagt cggggcgccg ccgggccggg tgcgacgcgg
     7321 cgagccgatc gcgggcgacg tcgcggacgc agcgcagcgg gttgagccag gcatggagcg
     7381 ccatcccccg cctgtgcgcc tcgtcgaccg cgaacgtgag cggatcccac cggggctcgg
     7441 gcggccgccc ctgctccccc gtaaggaacc gcgaccacgg ctcgaccgcc gagtcgtaca
     7501 tcgcgtcgca ctcggagcgg acctggaaaa agaccgcgtt gacgcccagg tcgccgagac
     7561 gatcgagcat ccgcacgagc tcggcccgtt gctcggccgg tgccaggcgc cccgacgacg
     7621 gccagtccag gttggccacg gtcgcgatcc atgccccgcg aaactcgtgc cgcggcgcat
     7681 cctgcgcgcg ggccgggcgg gccggccagg gggcgacgag aagcaggagg aagagcctgg
     7741 tcacgatggc ggaggacgga agcgcggcaa gctacgggta tc
//
LOCUS       DBNHQZ010000071         6211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_968428, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000071 DBNHQZ010000000
VERSION     DBNHQZ010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_968428"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16YU_01760"
     CDS             complement(<1..466)
                     /locus_tag="AC16YU_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6063975.1"
                     /translation="MIRRPLLLSALVLTLAAAWARPAAGQSDLDAVWAEMSRTVAEGD
                     FEGYAALYHDDAVLVSESAATSYPIAQALAGWKQGFDDTRAGRATAGVELRITRTLSD
                     GTAAHQTGMFRYSFHPEGGEASVITVHFEALLVKKDRWLMLMEYQQHAATDEE"
     gene            737..1417
                     /locus_tag="AC16YU_01765"
     CDS             737..1417
                     /locus_tag="AC16YU_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR6063976.1"
                     /translation="MNGAVTGFAHAALAPCLAGLLAVTAAAQSPLVRLSEVDSTIVQE
                     MRYFGSDNFIGRPIRGYRAPECMLTSAAAAALSRAQSAARAEGLGLLVYDCYRPQRAV
                     DDFVQWAYVLRDTARKALHFPDVPKTELFERGYISARSGHTRGSTVDLSLVRDGEPLD
                     MGTPFDFFDERSHTASPLVPEAATANRIRLRAVMEGSGFRNYASEWWHYTLVDEPYPD
                     TYFDFPVE"
     gene            1509..4178
                     /locus_tag="AC16YU_01770"
     CDS             1509..4178
                     /locus_tag="AC16YU_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6063977.1"
                     /translation="MTGTLFAHYEIEAELGRGGMGIVYRAHDTKLDRTVALKVLPSSA
                     LASSDDRTRFYREAKAAAQLHHPNIASVFEIDEAVPRAPGEEPQVGAEKRPYIAMEYI
                     EGETLEERIRKGPLGLADAVGVARQVAEALKAAHARNIVHRDVKSANVMLTADGVAKV
                     LDFGLAKTTQSTMLTRQGSTLGTVAYMSPEQARGLEVDGRSDLYSLGTVLYEMVAGRR
                     PFAGDYEQAVVYGILNEDPEPLTSLRTGVPMGLEWIVNKLLAKEARHRYQSAADLIAD
                     LETVDLSGSGFTRTRPAVAAPAAAARVPRKAWPWVAALGGALLVGLAIGWGIAREPDP
                     VVLPLAVTVQIPGEQRIGHPSLSPDARYLAFSASFNQKLLLHDLSDGTTRTIVDLTDA
                     RISRFTPNGKWLVFEHLAPAAGLSIVAVPDGNVARLVDSGRVPSWIDDEWLLYENGGA
                     IERISRITQVRDVIWEPGPDSGFRMDNPEVLPGQRHALVSLVSPTSASIAILDLGDGS
                     IRTLREGAVAPRYVSSGHLLFTEQVGPTVNYTGRLFAQPFDPASGTTRGPAVPVLGER
                     GYWDIGISAAGHLVTTGGLLADLSSSVFRLLWLDAESGLQELLGRTLPVVTGGLDLSP
                     DGTWLAYSTVSGGLLDGAVGIGPLDATLGAPTTTTLGMYTYSPRFEGDSESVLVNRGE
                     WSEGYSVYRVSASGASAPERLDLSESASEIFHYLSGDGRTLVLRAMDRTEPPFDATWW
                     LVDLESGSREQLGLPPEADGVDLSPDGRWLTYTLHEGGSRLFVMGIDGSGPWQVATDR
                     TYPQWLPGGRALAAIGSDGIYIIPLSMDRGVRPAGAERRVFTAIREFGFRVHRPTGRI
                     LVAERIVDEGGANNQIDLVLNWGERLRRDVPVQ"
     gene            4371..4826
                     /locus_tag="AC16YU_01775"
     CDS             4371..4826
                     /locus_tag="AC16YU_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIR6063978.1"
                     /translation="MEKLQLAPDFVEFLKLLNGNDVDYLVIGGYAVAVHGYVRTTGDL
                     DIWVRMSPANAARVVVALREFGFSTSELDDSLFLDPDKVTRLGIPPVQIEIFTSISGV
                     EFEQASIGRLQVALSDVEIPVIGFDDLIAAKKASGRHIDLHDVDRLLGG"
     gene            4869..>6211
                     /locus_tag="AC16YU_01780"
     CDS             4869..>6211
                     /locus_tag="AC16YU_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6063979.1"
                     /translation="MTGTHLAHYRIDAELGRGGMGIVYRAHDTKLDRTVALKVLPPHA
                     LASEDDRARFQREARAAAKLHHAHIASIFEIGEAVPSDAPHGSEPRPFIAMEFVDGES
                     LADRIAQGPLRLEEAVRLATQIAEALGAAHEAGIVHRDIKSGNVMLTAKGEVKVLDFG
                     LAKTAASTQLTRQGSTLGTVAYMSPEQARGEEVDGRTDLWALGVVLYEMVAGRLPFPA
                     DYEQAALYGILNEDPEPLTAIRTGVPMELEAVVFKLLRKEARLRYPSAAGLLADLETI
                     STGRTSSHRSVPAMPAAPSSGADTPSTRSWRMYALWFGFAGLLVGALVTASLLRAGSS
                     EVQSASEIVRSSIILPPEHPVQMSTGPTLGLEMDAIDLTRDGALMVYVSGTDDLPVLA
                     LRDMRDGTIRVLEGTDEAYNPTFSPDGRSIAYQTESGVYRVATDGGRPEFVAQTS"
BASE COUNT          941 a         2087 c         2180 g         1003 t
ORIGIN      
        1 actcctcgtc cgtggcggca tgctgctggt actccatcag catgagccag cggtccttct
       61 tcacgagaag ggcctcgaag tgcaccgtga tcaccgacgc ctcgccgccc tccgggtgga
      121 acgagtaccg gaacatcccc gtctggtgag cggccgtccc gtcggagagc gtccgcgtga
      181 tgcggagctc cacgccggcc gtcgcccgtc cggcgcgggt gtcgtcgaat ccctgcttcc
      241 atcccgcgag ggcctgcgcg atcgggtagc tcgtggccgc cgattccgac accagtacgg
      301 cgtcgtcgtg gtacagggcg gcgtagccct cgaagtcgcc ctccgcgacc gtgcgggaca
      361 tttccgccca gacggcgtcg aggtccgact ggccggcggc cggtcgcgcc caggcggcgg
      421 ccagggtgag gacgagtgcg gacaggagca gcgggcggcg gatcatggcg ggcgtgggag
      481 tgcgggagag gggccgcaac gtagggaggg cgaccgttcc gggcaaggcg ggggcgcggg
      541 tgtgcggtcg tggacgggag cggacacctt acccgatccg gtcacgggta aggcgtcgcc
      601 ttaccggaca ggccgccggg taaggccccg tgtacggccg gcgggcacgc gacgctccgc
      661 cggcgatacg cgtccagtca cactggatgc ggcgtcctgc ccgcctctat cttgcgggtc
      721 accagccatc cctgccatga acggtgccgt caccgggttt gcccatgccg ccctggcccc
      781 gtgcctcgca gggcttctgg ccgtgaccgc cgccgcccag tcgccgctcg tccgcctgtc
      841 ggaggtggat tccacgatcg tccaggaaat gcgctatttc ggcagcgaca acttcatcgg
      901 gcggccgatc cgcgggtacc gcgcgccgga gtgcatgctg acctccgccg cggccgcggc
      961 gctctcgcgg gcccagtccg cggcgagggc cgaggggctc ggactgctcg tctacgactg
     1021 ctaccggccg cagcgcgcgg tcgacgactt cgtgcagtgg gcatacgtac tgcgcgacac
     1081 cgcaaggaag gcgctccact tccccgacgt gcccaagacc gagctcttcg agcgcgggta
     1141 catctcggcg cgctcgggac acacccgcgg cagcaccgtc gacctgtcgc tggtccggga
     1201 cggcgagccg ctggacatgg gcacgccgtt cgatttcttc gacgagcgat cccatacggc
     1261 aagcccgctg gtcccggagg ctgcaacggc caaccgcatc cggctcaggg ccgtcatgga
     1321 gggctcgggc ttcaggaact acgcgagcga gtggtggcac tacacgctcg tggacgagcc
     1381 atatccggac acgtacttcg atttcccggt cgagtagacc cggcgcaggg acccgggctg
     1441 tgaagccctt gtgaacccgt cgcccgagcc gtttctttgg cccgcttccc ctgcacgcgc
     1501 aatcccagat gaccggcacc ctgttcgccc actacgagat cgaggcagag ctcggccgtg
     1561 gcggcatggg catcgtatac cgcgcgcacg acacgaagct cgatcggacg gtggcgctca
     1621 aggtgctacc ctcctcggcg ctcgcgagct ccgacgaccg gacgcggttc taccgcgagg
     1681 cgaaggccgc cgcgcagctg caccatccca acatcgcctc ggtcttcgag atcgacgagg
     1741 cggtgccgcg cgcgcccggg gaggagccgc aggtcggcgc cgagaagcgc ccctacatcg
     1801 cgatggagta catcgagggg gagacgctcg aggagcgcat ccggaagggc cccctcgggc
     1861 tcgcggacgc ggtcggcgtc gcgcgccagg tcgccgaggc gctcaaggcc gcgcacgcaa
     1921 ggaacatcgt tcaccgggac gtcaagtcgg ccaacgtgat gctcacggcc gacggcgtgg
     1981 ccaaggtgct ggacttcggt ctcgccaaga cgacccagtc cacgatgctc acgcgccagg
     2041 gctcgacgct tgggacggtg gcctacatga gccccgagca ggcccggggg ctggaggtcg
     2101 acgggcggag cgacctctac tcgctcggca ccgtgctgta cgagatggtg gccggccggc
     2161 ggccgttcgc gggggattac gagcaggccg tggtgtacgg catcctgaac gaggaccccg
     2221 agccgctgac ctcccttcgg accggcgtgc cgatgggcct ggagtggatc gtgaacaagc
     2281 tcctggccaa ggaggcgcgg caccgctacc agtcggcggc cgacctgatc gccgacctcg
     2341 agaccgtgga tctgtcgggc agcggattca cgagaacgcg ccctgcggtg gcagcgcccg
     2401 ccgctgcggc ccgcgtgccc aggaaggcgt ggccgtgggt ggcggccctc ggaggcgccc
     2461 tgctggtcgg actggcgatc ggctggggta tcgcgcgcga gcccgatccc gtcgtcttgc
     2521 ccctggcggt gaccgtgcag atccccgggg agcagcggat cggccacccg tcgctgtctc
     2581 cggacgcccg gtacctggcg ttctcggcct ccttcaacca gaagctgttg ctgcacgacc
     2641 tttcggacgg gacgacacgc acgatcgtag acctgacgga cgcgcggatt tcgcggttta
     2701 cgccaaacgg gaaatggctc gtcttcgagc acctcgcgcc cgccgcggga ctctcgatcg
     2761 tggcggtccc ggacggcaac gtggcgcgac tggtcgactc cggccgggtg ccgtcgtgga
     2821 tcgacgacga gtggctgttg tacgaaaacg ggggagccat cgagcggatc agccgcatca
     2881 cgcaggtgcg tgatgtgata tgggagccgg gcccggattc cggattccgg atggacaacc
     2941 ccgaagtgct tcccgggcag aggcacgcgc tggtctcgct cgtttccccg acatcggcgt
     3001 ccatcgccat cctcgacctc ggcgacggct cgatccgtac gctgcgcgag ggagccgtgg
     3061 ccccccggta cgtgtcctcc ggacatctcc tcttcaccga gcaggtcggg ccgacggtga
     3121 attacacggg ccgtctgttc gcccagccgt tcgatccggc gagcgggacc acgcgcggtc
     3181 cggcggtacc cgtactcggc gaacgggggt actgggacat cggaatcagt gccgcaggcc
     3241 acctggtcac gaccggaggt ctcctggccg acctgtcttc gtcggtcttc cgcctgctct
     3301 ggctcgatgc ggaatccggc ctgcaggagc tgctgggccg gacgctgccg gtggtcacgg
     3361 ggggactgga cctgtcgccg gacggtacat ggctcgccta ttccaccgtg tccggcggct
     3421 tactggacgg tgccgtggga atcgggccac tcgacgccac cctcggcgcg ccgacgacga
     3481 ccacgctggg gatgtacacc tacagcccgc gcttcgaggg cgacagtgaa tccgtgctgg
     3541 tgaaccgcgg cgagtggagc gaaggctact ccgtctacag ggtctccgcc agcggagcgt
     3601 cggcgcccga acggctggac ctgtccgagt cggcctccga gatcttccac tacctgtccg
     3661 gtgacggcag gacgctcgtg ctccgggcga tggaccgcac cgagccgccg ttcgacgcca
     3721 cgtggtggct cgtggacctg gagtctggca gccgcgagca gctcggcctt ccgcccgaag
     3781 ccgacggtgt cgatctgtct cccgacgggc gctggctcac ctacacgctc catgaggggg
     3841 gatcccgtct gttcgtgatg ggcatcgacg gaagcggccc ctggcaggtc gcgacggacc
     3901 ggacctaccc gcagtggttg ccgggcggtc gcgcgctcgc cgcgatcggc tcggacggca
     3961 tctacatcat tcccctgagc atggatcgcg gtgtccgacc ggcgggagcc gagcgccggg
     4021 tcttcaccgc catccgggag ttcggcttcc gggtgcatcg cccgaccggg cgaatcctgg
     4081 tggcggagcg catcgtggac gaaggcggtg cgaacaacca gatagatctc gtcctgaact
     4141 ggggcgagcg gctgcgccgg gacgtgccgg tgcaatagcg ggcgcggcga gccgggggct
     4201 gatgaggccc gggccggacc atcgtctatc tttgagcatc atgaagctgg atcgtacttc
     4261 gttctcggtg atctcgaagg aggaccttcc ctcggcggat cgggcgttct ggcgttcccg
     4321 gtccccgcgc gagcgcgtcg aagccctcga gcaaatgcgt cgcatggcct atggagaagc
     4381 tgcaactggc gccggacttc gtcgagttct tgaagttgct gaacgggaac gacgttgatt
     4441 acctcgtcat cggtgggtat gccgtggccg tgcacggtta tgtgcgcacc acgggtgatc
     4501 tggacatctg ggtacgcatg tctccggcga acgcggccag agtagtggtc gcccttcgcg
     4561 agttcgggtt ctccacttcc gagctggacg attccctgtt cctggatccc gacaaggtga
     4621 cgcggctcgg catcccgccg gtgcagatcg agatcttcac atccataagc ggagtggaat
     4681 tcgagcaggc atcgatcggg cgtcttcagg ttgcgctttc ggacgtcgag attccggtga
     4741 tcggattcga cgatctgatc gcggcgaaga aagcgagtgg gcgtcatatc gacctgcatg
     4801 atgtcgaccg gctgcttgga gggtagcccg gctgagccgt ttcttccggc cagctactcc
     4861 tgcatacgat gaccggcacc cacctcgccc attaccgcat cgacgccgag ctcggccgtg
     4921 gcggcatggg catcgtgtac cgcgcgcacg acacgaagct cgaccggacg gtggcgctca
     4981 aggtgctgcc gccccacgcc ctcgcgagcg aggacgaccg ggcgcgtttc cagcgcgagg
     5041 ccagggccgc cgcgaagctc caccacgcgc acatcgcgtc gatcttcgag atcggggagg
     5101 cggttccgtc ggacgccccg cacggttcgg agccgcgccc gttcatcgcg atggagttcg
     5161 tggacgggga gtcgctcgcc gaccgcatcg cgcagggtcc gctcaggctg gaagaggccg
     5221 tccgcctcgc gacgcagatc gccgaagcgc tcggtgcggc ccacgaggcg ggcatcgtgc
     5281 accgtgacat caagagcggc aacgtgatgc tgacggcgaa gggggaggtg aaggtgctcg
     5341 acttcgggct cgcgaagacc gccgcgtcga cccagctcac gcgccagggc tcgacgctcg
     5401 ggacggtcgc ttacatgagc ccggagcagg cccgcggcga ggaggtggac gggcggacgg
     5461 acctctgggc gctcggcgtg gtgctgtacg agatggtggc gggccggctt ccgttcccgg
     5521 cggactacga gcaggcggct ctctacggca tcctgaacga ggacccggag ccactgaccg
     5581 cgatccggac gggggtgccc atggagctgg aggcggtggt cttcaagctt ctgcgcaagg
     5641 aggcccggct ccgatatccg tcggccgcgg gactgctcgc cgacctggag acgatatcga
     5701 ccggacgcac ctcttcgcac cgttcggtgc ccgcgatgcc ggccgcgcct tcgagcggtg
     5761 cggacacccc ttcgacgcgc agctggcgca tgtatgccct gtggttcgga ttcgccggtc
     5821 tgcttgttgg cgcgctggtc acggcctcgc tgcttcgcgc cggttcgtcc gaggtgcagt
     5881 cggcttccga aatcgtccgc tcgtcgatca ttcttccccc cgaacacccg gtccagatga
     5941 gcaccggacc gactctcggt ctggagatgg acgcgatcga cctgacgcgg gacggggccc
     6001 tcatggtgta cgtatccgga accgacgact tgcccgtcct ggccctccgc gatatgcgtg
     6061 acgggacgat ccgcgtcctc gagggcacgg acgaggcgta caatccgacg ttctcacccg
     6121 acggtcggtc aatcgcctac cagaccgaat cgggcgtcta ccgcgtggcg acggacggtg
     6181 ggcgaccgga gttcgtcgcg cagacgtcgg a
//
LOCUS       DBNHQZ010000072        20188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3271214, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000072 DBNHQZ010000000
VERSION     DBNHQZ010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3271214"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16YU_01785"
     CDS             <1..759
                     /locus_tag="AC16YU_01785"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6063980.1"
                     /translation="GIDQFESVHAARIRVGDLPLHPVEPFRFQLGSSPDKLLEDYTLP
                     VMVLEDGAVATVDPLMGVESIDFGEPFGRLEAFHTAGGLSHLAERLLGRVRYLDLKSV
                     QWPGHAGRMRFLLGLGLGEDRSIDVRTHLTYRDILVRRMRQRLAPAGPDVVLARVAVQ
                     GTVDGRDRTLVFGVAEHAGPDPEDSAMRRCTAVPAAAVAAILASGAIGGGGAAPPESV
                     VPRAALLSAVRGRGIAISEQWFDGHVGVGDAASA"
     gene            complement(760..1458)
                     /locus_tag="AC16YU_01790"
     CDS             complement(760..1458)
                     /locus_tag="AC16YU_01790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6063981.1"
                     /translation="MDERDFLGRVAVVTGASGRLGRLVTAAFQEEGAQVAALVRTEAD
                     ARLVPVVPGSTVRTVACDVTDAASVQKAFARVAEEFGRVDALAHTVGGWARTPLLETP
                     AGEWERILRINLLSTWHCFREAARIMAAADGGGLVAIASSQGADRGIAGQSAYAAAKA
                     GVIRLVESAAEELASHGITARAVAPSTILYGGETSPGVPARTVVRLCLDCAHPDAVVP
                     SGSVLRAYGTAADP"
     gene            1830..9830
                     /gene="sov"
                     /locus_tag="AC16YU_01795"
                     /gene_synonym="sprA"
     CDS             1830..9830
                     /gene="sov"
                     /locus_tag="AC16YU_01795"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR6063982.1"
                     /translation="MPARDGGGRAIRTLAGLVSFAAVAAWVLVGRASPEPAGTGYRWQ
                     VAARADTDSVAVLLDSLALDAAARSAVRSAMLRDVLAARSDTDSVAARLDSLALDSTS
                     AVADTGLARHYLPTWRDWRSARSTPRRNRPLGAKFGTYWRQSVEFDTTARTFRVHEQV
                     GGEDVRIPVIYDMEGYRSVRLSRDLERNWRAMVVQRTRQQMQQNRGGLGFNIVVPGGR
                     QSAFTTIFGKPSVDLRVNGQADIKAGFNYRKSDQQVAITGRASQLDPDFKQDLRLGIN
                     GTIGDKLRIDVNYDSQNQFDYQNQLKLQYTGYEDEIIQSVEAGNVFLQTPSSLIRGGQ
                     SLFGIKSQFQVGGFHLTTVMSQQEGQSNTLNIEGGSEATNFDLKPTDYDESIHFFLSY
                     YFRNRFEDALSDPPNIRVADGFERITEMEVWRLMPTTPEEQNVRQVVAVVDLGEPVDV
                     LSEADAFTAAVLPTNTIDQYDDQPDGEVDLQLRDGDATPGSYLESVKALTASDYQIGK
                     FRKLERGRDYQVDEILGTLSLRSRLQESEALAVAYRFTAGGRNFQVGDFSTDTGGSDG
                     GQNEDKLFLKLIRPVQLRQPAPESDFNPAAWYLELRNIYRLPGRGIQANEFDLQIFYE
                     PPGKTASKTLPGVSSQETLLRQLGLDRVNADQALRPDDVFDYLVHFTIDPGEGTLIFP
                     YLEPFGERLTDLIALTGGTDAEQEELRNLYVFETLYQEKKANARRDSQHDVFRIRGSY
                     KGSVQDFYDLRAYAGLIPGSVRVSSGGTPLQEGTDFTVDYQGGTVMIINPAFLTAGRS
                     INIDYEQNSLFNLQKKTLLGARMDYNLNDRLVLGATLMQMSQKSPIDKFRIGEEPVSN
                     TIWGVDGSYQAEPRWLTRMVDALPFIQTKERSAFSLTGEFAQLRPNHVETIAFERTRR
                     ELQKSGRDFTGDELGGISYIDDFEGFENTYSLMQPGTWTLSAAPDSIGAIDRGGSLPG
                     TLADSLRTTWRGSFAWYRANANILQEIPAVSYDADAIRPLRINDVFPNRDTRGELVDQ
                     LETLDIYFSPRERGPYNYTTDLRGFLSAPTDVWGGMTQRLPEGYTDFSLKNIDFVEFV
                     FRPFPENPARDAGTAARLYVDLGSISEDVLPDEKLSNEDGLSLATIGETSVTKWGRTP
                     SATQNSVVDVDDATSRTEDLGLDGLASYGGDYPPFATEQVHFSDFLDALDPSETDPAY
                     RAEMAKAMRDPSGDDYHYFGNSQYFDNPEFYPSGATFQQRFSRYFAGYELNAFETQNK
                     LATNTSVKRGNSRFPDSEDRNLNSTVDTDNSYFQYELPLSRAVLDSLAQPGRVDDYIV
                     GEITDRDGNGTGWYQARIPVQNFTRQVGEIQDFSLIESIRLWTDGHEVPVTIRFATLE
                     LVGSQWQKSTRIPLEHETPADTTASETRLTISSINNEENADLYSPPVGAVVSQTRLAT
                     GRVQNAREQSLVLRVENLYPGKQRAVFRTQNTALDLLRYSNVRMFVHMHGRTGDGTEL
                     YNLPIDEARSKVRLFVRLGANESNDYYEYEQPLTPSSETAGSSDALWQTAVDYQGRIQ
                     DLNSVNILLGALNQLKVTRDRLLFPTDSVFYNVENGVLVAPDAPDAESFGPPGTRIGI
                     KGTPSLGRVNSIVIGIRNPADSLAMGFEHVLEDVTVWVNELRVSGYDESNGWAGLANV
                     DLQLADFARIKANVQTQTDGFGSLSSTLAEREQNNLLNYSVTSEVNVDKLIPERFGWS
                     MPVNVQVQSNTTTPRFAPTRGDVRLSELLAQIDERTDIDEAQKEQLRVSANEEAQTHT
                     LSRSVTARVGKSGSRSAILRHTLDGLSFSWSNSQADGRTPQLVLNGTRRWSSTAAYRL
                     GVRRTRTIRPLFFLDYVPVLKVLGGLRWNLVPQSVSVSGSLNRQLNQTQERPTTTRAG
                     AVEQAPNIVRFPLREQHTFGHTRDFAIQYNPFSFLNLGFDTSTQQNLNAAGVDTLFNT
                     ITRDGAILAGYRIEQALAGGLVDSTDLATTWEQELLRVKPFDRVIREALSGAATVRAE
                     RHDQRFTGTFRPRLNTIRAIDFINLQDISYTATYGWQNGPVGRNTGASVRVMGDLRAG
                     VTLRVQEIWRKFGFYRSIETSQREYQAARDRERQDREREKQDRKRREDARKVEAEARE
                     KAAKELEAALETAELEAREAGFDLSRRAIDSIRAAIPQPPPPRPEEPDTTGGGGFRVP
                     LPNMGSVARQFILAVTGIRDVSLTYSGRRGGASSNVGIPVLGADSSIVDVRTPYSLLD
                     AIRGDGVPLRYRFGLYRFLDSSERLVDPSLQVADNIETTDQLQGRTLISPWQSVQLNV
                     TWNVEWTQGQTYTYRPAFDAANRYVGIDTTLTENGSNRSSIWAFGASYLDLFYRQYQT
                     LLYDLQSAGPDATEFGDANGDGRVVLTNATVTEDFRKAFVMGGGRTLDRLRLMPTPLP
                     NWTLTYSGLSKWPVFRSLVTNLTVRHGYTADYSADYATNSSLSGGEPFRTLDLGNRRI
                     IFAIEPYQTGSMRVNERYMPLVGVDVAWKGQIQTNVAFNRSNAYSLSTSNFEVSENRT
                     NEISFTASWQKSGMKIPLFGKRLNNRVSFSLNVAHSETRDQRLRLRRALESALLDPNF
                     VPANALEGDNISLVTAHTRLTVSPTIAYQFSNRVTANFTLSYEKFDSEDSRQPSSVNI
                     QGNFNIRVSISN"
     gene            9830..10660
                     /gene="lipB"
                     /locus_tag="AC16YU_01800"
     CDS             9830..10660
                     /gene="lipB"
                     /locus_tag="AC16YU_01800"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6063983.1"
                     /translation="MADQDTRRETLPRPAAAGGRQRVVVCDLGRVSYGTAWDLQARLQ
                     ARLVAAKRGEAPPAPHVLLLVEHPPVVTIGKSGDATHVLFDEEALARRGIGLYRVDRG
                     GDVTYHGPGQLVAYPILDLDRFFTDIHRYLRELEEAVIGVCSDLGLAAGRIEGRTGVW
                     IGPDDRGPERKVCAMGIRCSRWVTMHGLALNVSTDLTHFDVIVPCGIGDRGVTSLARE
                     TGRTVDLAEASERLVGRFAERFGVDVERLAGEPARAFLGDLLGAPLDARDFRSAAAAF
                     "
     gene            10774..11133
                     /locus_tag="AC16YU_01805"
     CDS             10774..11133
                     /locus_tag="AC16YU_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063984.1"
                     /translation="MANFHLEDFARRLIAETLFYDEEYEALGNLSLVDAAATREAFVA
                     SYAPEDGVFVIEEATEWEEYEPGDADDIGYALAVDSREHGTYDTPDEAAAALLDLARK
                     HNLAPSITLLFEEDDAG"
     gene            complement(11099..11497)
                     /locus_tag="AC16YU_01810"
     CDS             complement(11099..11497)
                     /locus_tag="AC16YU_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR6063985.1"
                     /translation="MVNRRPFIVIGSVLAGLAVAAGAFGAHGLRDVISPERLDVFDTA
                     VRYQMMHALAMLLVGVLGAESGNRLLRAAGWCFALGIVLFSGSLYTLVLTGVAALGAV
                     TPFGGVFFLAGWGLIAAWGISLRRPPRTEA"
     gene            11600..12055
                     /locus_tag="AC16YU_01815"
     CDS             11600..12055
                     /locus_tag="AC16YU_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063986.1"
                     /translation="MAFTFSDADIDRIAAVLEAEPKRDGSLVRFELTDDESGRRLALE
                     IRTSLDLPQRAVADSVSNLVSVYAASSFLQIQGCTGFIASQELGEVIFVARSGHAANG
                     LVVERQAGCSLYANVHPRLLSADFTQLPPELIMSSVALSMAEDLFGDLG"
     gene            12066..12533
                     /gene="ybeY"
                     /locus_tag="AC16YU_01820"
     CDS             12066..12533
                     /gene="ybeY"
                     /locus_tag="AC16YU_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6063987.1"
                     /translation="MTDRPTRDAAPTGPDPGLEVSCVHPERHAPGVDLEALARRVLEG
                     ERCAWVYVGIVLADHALVHRLNREYLGHDFRTDVLSFLIDRTDRGIEGEVYVDLDTAE
                     SNAARFGEPYEREVARYVVHGLLHLSGHDDSTEEERARMRLLEDRYLGPVAVD"
     gene            12593..12904
                     /gene="hpf"
                     /locus_tag="AC16YU_01825"
     CDS             12593..12904
                     /gene="hpf"
                     /locus_tag="AC16YU_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6063988.1"
                     /translation="MQIQVTARHFDADDSLKEYVQMKVARLQRYYDGITDVQVVLTRN
                     AQVPEALAEIVVGVFRKQLTARAESATHEEAIDRSVERIRRQLYRYKARLRGTDRDEH
                     R"
     gene            complement(12962..13918)
                     /locus_tag="AC16YU_01830"
     CDS             complement(12962..13918)
                     /locus_tag="AC16YU_01830"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6063989.1"
                     /translation="MKVAELLERASAPLVSYEIIPPRRGGSLQGILDIVEQLVEFEPP
                     FIDVTSHSAQAYYEEQPDGSWRRLIKRKRPGTLGLCASIRGRYGIETVPHILCLGFTR
                     EETEDALIELNYLGIRNVMALRGDDHGVGKPIRPDRTSNEYAVDLVRQIVDMNRGRYL
                     EELVDAAATDFCVGVAGYPEKHFESPNLTWDIQKLKQKVDAGAHYVTTQLFFDNDKYL
                     AFVGKCREAGITVPIVPGLKILTSKRQVSSLPSRFHVSIPEELAGEVLEAPDGHVAAV
                     GVEWARRQAVGLLEAGVPYLHFYIMATADHVRTVVQDLRRLA"
     gene            complement(13970..14278)
                     /locus_tag="AC16YU_01835"
     CDS             complement(13970..14278)
                     /locus_tag="AC16YU_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063990.1"
                     /translation="MSLLLVLVAAPTVAVMTVWASMTALRILRPEDSVLRPLDPVLVQ
                     LARMPVRPAVAAGDDAPPAWFSGRDTWREFHHARYDYAPGRSDGLPEAWWEDVVRRQN
                     "
     gene            complement(14289..14699)
                     /locus_tag="AC16YU_01840"
     CDS             complement(14289..14699)
                     /locus_tag="AC16YU_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR6063991.1"
                     /translation="MAHADGHTHHITPTRTLVAVFAGLVVLTIVTVAVSRVHLGPLNV
                     PIALAIAAAKGTLVVLFFMALKYDNRVNMVILTVGALFVVVFLAFTLLDTQFRGDLPN
                     TTKGTIMDQAREEEALKAREPDPESIRINRPDTP"
     gene            complement(14705..15400)
                     /locus_tag="AC16YU_01845"
     CDS             complement(14705..15400)
                     /locus_tag="AC16YU_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HIR6063992.1"
                     /translation="MATPTATTLEHDAEPPAHLQHHFVTSDQQFDAAKMGMWLFLITE
                     ILLFAGMFVAYAVYRAWHPEVFQHSADLLDWRLGGLNTIVLLLSSFTVAYGINRIQHD
                     DRRGLVINLVITLACAAVFMIVKYFEYTGKFQAGIFPGEAFAPVGEYHGHDLALYDIP
                     YAPQFFSIYFVMTGIHGVHVLVGMGVFTWILVKALRGRFSSAWYTPVELTGLYWHLVD
                     IIWIFLFPLLYLI"
     gene            complement(15451..17154)
                     /gene="ctaD"
                     /locus_tag="AC16YU_01850"
     CDS             complement(15451..17154)
                     /gene="ctaD"
                     /locus_tag="AC16YU_01850"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6063993.1"
                     /translation="MNESNPSAPIHGAVNYLNHERGLKSWLLTLDHKRIGLMYLAAVS
                     LAFLSGGILALLVRVELLAPGETIMGPDAYNQSFTLHAVLMIFAFIIPAVPAVMGNFV
                     LPMQLGAKDVAFPRLNLASWYIYILGALIVMSALLVGRIDGGWTFYAPYSERVGDSIL
                     FVTLGVFVMGFSSILTGLNFIVTIHKMRAPGLTWTRLPLFVWSIYATSIVQVLATPVI
                     GITMVLLFLERLLGIGIFDPALGGDPVLFQHFFWFYSHPAVYIMILPAFGIISDLMGT
                     FSRNEVQGYKFVALSSVAIAFLGFLVWGHHMFVSGQSATSSIVFSLITYLIGIPTGVK
                     VLNWVGTMYRGSVWLRTPMLYALAFLFTFSIGGFTGIMLGVLAIDVHLHDTYFVVAHF
                     HYVMMGGNVIAALGGLHYWWPKMTGKMFNETAGRIAAALVFVGFNLTFFPQFILGSQG
                     MPRRYYDYLPEFQPLHELSTYGSWVLSAGLFLILGYLLASLFSKKRAPANPWGALTLE
                     WTHTTSPPHPHNFETTPIVTRGPYDYHLAPELFGDGPAGDGAGTATPPVRPAERESTE
                     A"
     gene            complement(17197..18129)
                     /gene="coxB"
                     /locus_tag="AC16YU_01855"
     CDS             complement(17197..18129)
                     /gene="coxB"
                     /locus_tag="AC16YU_01855"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6063994.1"
                     /translation="MGENGNVWMPDARSTVAPEVDALFYFVFWTSLIIFAMVMAAMVY
                     FAFRYRRRNAHEVPAPVHESRLLEVAWVVLPTILVLIVFTWGFKVYVHLYSPPPDSYQ
                     ITVRGKQWFWEFEYPNGTVTTNEMHVPIGVPVKLNMSSEDVLHSFFVPEFRVKQDVLP
                     NRYTSLWFEPTEIGEYQIFCTEYCGTSHSGMLGKVVVETEEGFQAWLAAQGADRPPLE
                     LGQDLFRQYNCNVCHSVDGTRIVGPSLKGLYGAERRFEDGTTAVADENYIRESILAPG
                     ARIVETYPNAMPATFGSLSAKQLDGLIAFIQSLE"
     gene            complement(18154..18987)
                     /locus_tag="AC16YU_01860"
     CDS             complement(18154..18987)
                     /locus_tag="AC16YU_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6063995.1"
                     /translation="MTRFGALFGVLLAWGTVSGASLSAAQPAGSIPSVFDEVGIDERL
                     GDRLPLDARFEDETGRTVALGEYFDGERPVVLNLVYHECPMLCSLLLTEFTRTLSEME
                     WQPGGEFDVVTVSFSANEAPTIAARAKEKYLAQLGRPEAGAGWHFLTGEHESIQVLAD
                     ALGFRFRWVESTREFAHPAALIFASGDGMITRYIHGMGYPPADVRKALVEASEGRVGS
                     AVDRILLYCYRYDADANSYVLHARNLMKLGGLLTLLLLVVGMALLWRREHGPLRPAGA
                     A"
     gene            complement(19010..19378)
                     /locus_tag="AC16YU_01865"
     CDS             complement(19010..19378)
                     /locus_tag="AC16YU_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063996.1"
                     /translation="MTSVPVHDEHADSGFEPEGVSGLGILGLTVAGGLILAVLVLIAV
                     QLARSEFRAAEGEAVALTGYPALVETRIAGEEKLTQYGVVDAAARRYRIPVDRAIDLM
                     VNEAAAEGDRPVSEEVRLTR"
     gene            complement(19553..>20188)
                     /locus_tag="AC16YU_01870"
     CDS             complement(19553..>20188)
                     /locus_tag="AC16YU_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063997.1"
                     /translation="VTTAFASFDLLMSLDPHWFSTIFGVYFFGGSFLGIMAFMILLAL
                     VYQRGGMLREGVSAEHYQDLGKFMFGFVVFWAYIAFSQYMLIWYGNLPEETVWYRHRL
                     EHGWELHSVTLLIFHFAIPFVVLISRWAKRMLGLLAFMSVWLLFMHWFDLHWIVMPVL
                     HEEAGFHLLDFTALLGLGGIWVAVLIFRMSRHSMVPQRDPRLAQSLGFLNS"
BASE COUNT         3546 a         6989 c         6645 g         3008 t
ORIGIN      
        1 ggaatcgacc agttcgagtc cgtccacgcg gcccgcatcc gcgtcgggga ccttccgctc
       61 catcccgtgg agccgttccg cttccagctg ggatcctccc cggacaagct gctcgaggac
      121 tacaccctgc cggtgatggt gctcgaggac ggtgcggtcg ccacggtgga tccgctcatg
      181 ggcgtcgaat cgatagattt cggggagccg ttcgggcggc tggaagcctt ccacacggcg
      241 ggcggcctct cgcacctggc ggagaggctg ctgggccggg tccggtacct ggacctcaag
      301 tccgtccagt ggcccggaca cgcgggccgc atgcggttcc tgctcgggct gggactcggc
      361 gaggaccgct cgatcgacgt gcgcacccac ctgacgtacc gggacatcct ggtgcgccgg
      421 atgcggcagc ggctcgcacc cgccggaccg gacgtcgtgc tggcgcgcgt cgccgtccag
      481 gggaccgtcg acggccgtga tcgcacgctc gtgttcggtg tcgcggaaca cgcgggcccc
      541 gaccccgaag actccgcgat gcggcgctgc accgcggtgc ccgcggcggc cgtggccgcg
      601 atattggcct cgggcgcgat cggtggcggc ggggccgctc ccccggaatc ggtcgtgccc
      661 agggcggcat tactttccgc ggtccgcggc cgcggcatcg cgatcagcga gcagtggttc
      721 gacggtcacg tgggcgtcgg cgacgccgcg tcggcctgat caggggtcgg ccgccgtgcc
      781 gtacgcccgc aacacggatc ccgacggcac gacggcatcg gggtgggcgc agtccaggca
      841 caggcgcacg accgtgcggg cgggcacgcc cggcgaggtc tccccgccat acaggatcgt
      901 ggagggggca accgcgcggg ccgtgatgcc gtgggaggcc agttcctccg ccgcggactc
      961 cacgagtcgg atcacgcccg ccttggccgc cgcataggcc gactgtccgg cgatcccgcg
     1021 gtccgctccc tgcgacgagg cgatcgccac gagacctccc ccgtccgccg ccgccatgat
     1081 gcgcgccgcc tcccggaagc agtgccacgt ggaaaggagg ttgatccgca ggatccgttc
     1141 ccattccccg gctggcgttt cgagcagggg cgtccgcgcc cagccgccca cggtgtgcgc
     1201 gagggcgtcc acgcgaccga attcctccgc gaccctcgcg aacgccttct ggacggaggc
     1261 cgcgtccgtc acgtcgcacg ccaccgtgcg gaccgtcgag ccgggcacga cgggcaccag
     1321 cctcgcgtcc gcctcggtac ggacgagggc cgccacctgg gcgccctcct cctggaaagc
     1381 cgccgtcacc aggcgcccga gccgtcccga agcgccggtc acgaccgcca ccctgcccag
     1441 gaagtccctt tcgtccatgc gttcctccga tgtggccgcc ggcgcattcc ggtcggcgtt
     1501 gcgctgcccg gaccgtgaac aaaaatacat caggcctgtt cggattgtcc ggcgccaccc
     1561 acaggcacga ctttcgaacc ttgaagtggc ttgcaggcat gtgtagcttg cagccggcac
     1621 gcccgggaat ccggcccccg tgccggatcg tccggtccca ccgcaccccg caccaaatcc
     1681 gcactccgct ccgcccgtca ccgctaccga ccgatccatt catccgtaac cggacggcct
     1741 gctgactcca gacgcggaac gccttcgtgc cccgggacca ctccgatccg aaatcaacag
     1801 tcgatgcccc ccgtcggcac gacccggccg tgcccgcccg tgacgggggc ggtcgggcga
     1861 tccgtacgct tgcgggcctc gtgtcgttcg cggccgtcgc cgcctgggtg ctggtaggac
     1921 gggcttcgcc cgagccggcc gggacaggct accgctggca ggtcgccgca agggcggaca
     1981 cggattcggt cgccgtcctg ctcgactcgc tcgccctgga cgcggccgct cgctccgccg
     2041 tccggagcgc catgctgcgg gacgtgcttg ccgcgcggtc cgacaccgac tcggtggccg
     2101 cccggctcga ctcgctcgcc ctggactcga cctcggccgt ggcggacacg gggctggccc
     2161 ggcattacct gcccacgtgg agggactggc ggagcgcccg ttcgactccc aggcgaaatc
     2221 gtccactggg cgcaaaattc ggcacctact ggcggcagag cgtggaattc gacacgaccg
     2281 cgcggacgtt ccgggtgcac gagcaggtcg gcggcgagga cgtccgcatt ccggtgatct
     2341 acgacatgga ggggtaccgg tccgtgcggc tctcccgcga cctcgagcgc aactggcggg
     2401 ccatggtcgt gcagcgcacc cggcagcaga tgcagcagaa ccgcgggggc ctcgggttca
     2461 acatcgtcgt gccgggaggg cgccagagcg cgttcacgac gatcttcggc aagccctcgg
     2521 tggacctccg ggtgaacggc caggccgaca tcaaggccgg cttcaactac cgcaagagcg
     2581 accagcaggt ggcgatcacc ggaagggcct cgcagctcga ccccgacttc aagcaggacc
     2641 tgcggctggg catcaacggg acgatcggcg acaagctccg gatcgacgtc aactacgact
     2701 cgcagaacca gttcgactac cagaaccagc tcaagctgca gtacacgggc tacgaagacg
     2761 agatcatcca gtcggtcgag gccggcaacg tgttccttca gaccccctcc tcgctgatcc
     2821 ggggcgggca gagcctcttc ggcatcaaga gccagttcca ggtcggcggc tttcacctca
     2881 ccacggtgat gagccagcag gaggggcagt cgaacacgct gaacatcgag ggcggctccg
     2941 aggccacaaa cttcgatctc aagccgacgg actacgacga gagcatccac ttcttcctgt
     3001 cgtactattt ccgcaaccgc ttcgaggacg cgctctcgga cccgcccaac atccgcgtgg
     3061 cggacggttt cgagcggatc acggagatgg aagtgtggcg gctcatgccc acgacgcccg
     3121 aggagcagaa cgtccgccag gtggtcgcgg tcgtggacct cggcgaaccg gtcgacgtcc
     3181 tctcggaggc cgacgccttc acggcggccg tgcttccgac caacacgatc gaccagtacg
     3241 acgaccaacc ggacggggag gtggacctgc agctgcgcga cggcgacgcc acgcccgggt
     3301 cgtacctgga gtccgtgaag gccctcacgg cgtcggacta ccagatcggc aagttccgga
     3361 agctcgagcg cgggcgcgac taccaggtcg acgagatcct gggcacgctg agcctcaggt
     3421 cccgcctcca ggagagcgag gccctcgcgg tggcctaccg cttcaccgcg ggcggccgca
     3481 acttccaggt gggcgacttt tcgacggaca ccggcggctc ggacggcgga cagaacgaag
     3541 acaagctgtt cctgaagctg atccggcccg tgcagctccg gcagcccgca cccgaatccg
     3601 acttcaaccc ggccgcctgg tacctggagc tgcgcaacat ctaccggctc cccggccgcg
     3661 gaatccaggc caacgagttc gacctccaga tcttctacga gccgcccggc aagacggcgt
     3721 cgaagaccct tcccggggtc agctcgcagg agaccctgct gcggcagctc ggcctggacc
     3781 gggtcaacgc cgaccaggcg ctccggcccg acgacgtttt cgactacctg gtccacttca
     3841 cgatcgaccc gggcgaggga acgctcatat tcccgtacct ggagccgttc ggcgagcgcc
     3901 tgaccgacct cattgccctg acgggcggaa cggacgcgga gcaggaggag ctgcgcaacc
     3961 tgtacgtgtt cgaaaccctg tatcaggaga agaaggcgaa cgcccgccgg gactcccagc
     4021 acgacgtctt ccgcatccgg ggctcgtaca agggctcggt ccaggacttc tacgacctgc
     4081 gcgcctacgc cggcctgatc ccgggctccg tgcgcgtgag ctccggcggc acgcccctgc
     4141 aggagggcac cgacttcacg gtcgactacc agggcgggac cgtgatgatc atcaatccgg
     4201 cgttcctcac ggccggccgg tccatcaaca tcgactacga gcagaactcg ttattcaacc
     4261 tccagaagaa gacgctcctc ggagcgcgga tggactacaa cctgaacgac cgcctcgtgc
     4321 tcggcgccac cctcatgcag atgagccaga agtcgccgat cgacaagttc cggatcgggg
     4381 aggagccggt ctcgaacacc atctggggcg tggacggctc gtaccaggcc gagccccggt
     4441 ggctcacgcg catggtcgac gcactgccct tcatccagac gaaggagcgg agcgcgttct
     4501 cgctgacggg ggagttcgcc cagctccgtc ccaaccacgt cgagacgatc gcgttcgagc
     4561 gcacccggcg cgagctccag aagtccgggc gcgacttcac gggcgacgag ctcggcggca
     4621 tctcgtacat cgacgatttc gagggcttcg agaacaccta ctcgctcatg cagccgggta
     4681 cgtggaccct cagcgccgcg cccgattcca tcggtgcgat cgaccgcggc ggctccctcc
     4741 ccgggacgct cgcggactcg ctgcgcacca cgtggagggg cagtttcgcg tggtaccgcg
     4801 ccaacgcgaa catccttcag gaaattccgg ccgtctcgta cgacgccgac gccatccgcc
     4861 cgctgcggat caacgacgtg tttccaaacc gcgacacgcg cggcgagctc gtcgaccagc
     4921 tcgagacgct ggacatctat ttcagcccgc gcgagcgcgg cccgtacaac tacaccaccg
     4981 accttcgtgg cttcctctcg gcgccgaccg acgtctgggg cggcatgacc cagcgtctcc
     5041 ccgaggggta cacggatttc agcctcaaga acatcgactt cgtcgagttc gtcttccggc
     5101 cgttcccgga gaacccggcg cgggacgccg ggaccgcggc gcgcctgtac gtggatctcg
     5161 gctcgatctc ggaggacgtg ctgccggacg agaagctgtc gaacgaggac ggcctgtccc
     5221 tcgccacgat cggcgagacg tccgtcacga aatggggccg cacgccgagc gccacccaga
     5281 acagcgtcgt cgacgtcgac gatgccacca gccgcacgga ggacctgggg ctcgacggcc
     5341 tggcctcgta cggaggggac tatccgccgt tcgcgaccga gcaggtgcac ttcagcgact
     5401 tcctcgacgc gctcgatccg tccgagaccg atcccgcgta ccgcgccgag atggccaagg
     5461 ccatgcggga cccctccggc gacgattacc actacttcgg caacagccag tacttcgaca
     5521 accccgagtt ctacccctcg ggggccacgt tccagcagcg gttcagccgg tatttcgccg
     5581 gctacgagct caacgcattc gagacccaga acaagctggc gacgaacacc tccgtcaagc
     5641 gcggcaactc gcgcttcccg gactccgagg accgcaacct gaactcgacg gtcgacaccg
     5701 acaacagcta tttccagtac gaacttccgc tcagccgggc cgtgctggac tcgctggcgc
     5761 agcccggtcg tgtcgatgac tacatcgtgg gcgagatcac cgaccgggac ggcaacggaa
     5821 cgggatggta ccaggcgcgg attcccgtgc agaacttcac ccggcaggtg ggcgagatcc
     5881 aggacttttc gctcatcgag tccatccggc tctggacgga cggccacgag gttccggtca
     5941 ccatccgctt cgccacgctc gagctggtcg gcagccagtg gcagaaatcg acgcggatcc
     6001 cgctcgagca cgagacgccc gccgacacga ccgcatcgga aacccggctc accatctcga
     6061 gcatcaacaa cgaggagaac gccgacctgt actctccgcc cgtcggagcc gtcgtgagcc
     6121 agacgcgcct cgcgaccggc cgcgtgcaga acgccaggga gcagtcactc gtgctccgcg
     6181 tcgagaacct gtacccgggc aagcagcgcg cggtcttccg gacgcagaac acggccctcg
     6241 acctgctccg gtattcgaac gtccgcatgt tcgtccacat gcacggccgg accggcgacg
     6301 ggaccgagct gtacaatctc ccgatcgacg aggcccgctc caaggtgcgc ctcttcgtgc
     6361 ggctgggcgc gaacgagagc aacgactact acgagtacga gcagcctctg acgccgagct
     6421 ccgagaccgc gggctcgagc gatgcgctct ggcagacggc cgtcgactac caggggcgga
     6481 tccaggacct gaactcggtg aacatactgc tcggagcgct caaccagctc aaggtcacgc
     6541 gcgaccggct cctcttcccg acggacagcg tcttctacaa cgtcgagaac ggagtcctcg
     6601 tggcgcccga cgcgcccgac gcggagtcct tcggcccgcc cggcacccgc atcggcatca
     6661 agggcacgcc ctcgctcggc cgggtcaaca gcatcgtcat cggcatccgg aacccggccg
     6721 actccctggc catgggcttc gagcacgtgc tggaggacgt gacggtctgg gtgaacgaac
     6781 tccgggtcag cgggtacgac gagtcgaacg ggtgggccgg actcgccaac gtggacctcc
     6841 agttggcgga ttttgcccgg atcaaggcga acgtccagac ccagaccgac ggcttcggct
     6901 ccctctcgtc cacgctcgcg gagcgggagc agaacaacct gctcaactac agcgtgacgt
     6961 ccgaggtcaa cgtcgacaag ctcataccgg aacggttcgg ctggagcatg cctgtgaacg
     7021 tgcaggtcca gtcaaacacg accacgcccc gtttcgcgcc gacgcgaggc gacgtccgcc
     7081 tgtccgagct gctcgcgcag atcgacgagc gcacggacat cgacgaggcc cagaaggagc
     7141 agcttcgcgt ctcggcgaac gaggaggcgc agacgcatac cctgtcgcgc tcggtcacgg
     7201 cgcgcgtcgg caagtcggga tcccgctcgg cgatcctccg gcacacgctc gacggcctct
     7261 cgttctcctg gtcgaacagc caggcggacg gccgcacccc gcagctggtc ctgaacggga
     7321 cgcgccggtg gtcctcgacc gccgcgtacc gcctgggcgt ccgccgcacc cggaccatcc
     7381 gaccgctttt cttcctggac tacgtgcccg tgctgaaggt gctcggcggc ctgcggtgga
     7441 acctggttcc gcagtccgta tcggtctcgg ggtcgctgaa ccgtcagctg aaccagaccc
     7501 aggaacggcc gacgacgacc cgggcgggag cggtcgagca ggcgcccaac atcgtccggt
     7561 tcccgctccg cgagcagcac acgttcggcc acacgcgcga cttcgccatc cagtacaatc
     7621 cgttcagctt cctgaacctc ggattcgaca cgagcacgca gcagaacctc aacgccgcgg
     7681 gcgtcgacac gctgttcaac acgatcaccc gcgacggtgc catactggcc gggtaccgga
     7741 tcgagcaggc gctcgccggc ggcctcgtgg acagcacgga tctcgccacc acctgggagc
     7801 aggagctgct ccgcgtaaaa ccgttcgacc gggtgatccg cgaggcgctt tccggggccg
     7861 caacggtccg ggcggagcga cacgaccagc ggttcaccgg gacgttcagg cctcgcctga
     7921 acacgatccg ggccatcgac ttcatcaacc tccaggacat ctcgtacacg gcgacctacg
     7981 ggtggcagaa tggacccgtc ggacggaata cgggagcgtc cgtccgcgtc atgggcgacc
     8041 tgcgcgccgg cgtcacgctg cgcgtgcagg agatctggcg caagttcgga ttctaccgct
     8101 ccatcgagac cagccagcgc gagtaccagg ccgcgcggga ccgcgagcgg caggatcgcg
     8161 agcgggagaa gcaggaccgc aagcgccgcg aggatgccag gaaggtggag gcggaggcac
     8221 gcgagaaggc cgcgaaggag ctcgaggcgg ccttggagac ggccgagctg gaggcccgcg
     8281 aggcgggctt cgatctgagc cggagggcca tcgattccat ccgggcagcc atcccccagc
     8341 ctccgccgcc gcggccggag gagcccgaca ccacgggcgg aggcggtttc cgggttccgc
     8401 tcccgaacat gggctccgtg gcgcgccagt tcatcctcgc cgtgaccgga atccgcgatg
     8461 tgtccctcac gtacagcggc cgtcggggcg gtgcctcgtc gaacgtcggc atcccggtcc
     8521 tcggcgcgga ttcctcgatc gtggacgtca ggaccccgta cagcctgctc gacgccatcc
     8581 gcggggacgg cgtgccgctc cggtaccggt tcggcctgta ccgcttcctg gactcgagcg
     8641 agcgcctcgt cgatccgagc ctccaggtgg cggacaacat cgaaacgacc gaccagctcc
     8701 aggggcggac cctcataagc ccctggcagt cggtgcagct gaacgtcacg tggaacgtgg
     8761 aatggacgca ggggcagacc tacacctacc gtccggcgtt cgacgctgcc aaccggtacg
     8821 tggggatcga cacgacgctc acggagaacg gctccaaccg ctcctcgatc tgggccttcg
     8881 gcgcctccta cctggatctc ttctaccgtc agtaccagac gctgctctat gacctgcagt
     8941 ccgccgggcc tgacgccacc gagttcggcg acgcgaacgg cgacggccgg gtggttctca
     9001 cgaacgcgac cgtgacggag gacttccgga aggccttcgt catgggcggc ggacggactc
     9061 tggaccgcct gcgcctgatg cccacccccc tgcccaactg gaccctgacg tactcgggac
     9121 tctccaagtg gccggtcttc cggtccctcg tcaccaacct caccgtccga cacggctaca
     9181 cggccgacta ctccgcggat tacgccacca actcgtccct ctccggaggg gagcccttcc
     9241 ggacgctgga cctcggcaat cgccggatca tcttcgccat cgaaccctac cagacgggct
     9301 ccatgcgggt gaacgagcgc tacatgcccc tcgtcggcgt ggacgtcgcg tggaagggcc
     9361 agattcagac caacgtggcg ttcaaccgct cgaacgccta ctcgctttcg acgagcaatt
     9421 tcgaggtcag cgaaaaccgc accaacgaga tttcgttcac cgcttcgtgg cagaagagcg
     9481 gcatgaagat ccccctgttc ggcaagcggc tgaacaaccg ggtcagcttc tcgctcaacg
     9541 tggcacactc cgagacgcgc gaccagaggc tccggctccg cagggccctc gaatcggccc
     9601 tcctcgaccc gaacttcgtt cccgcgaatg ccctggaagg cgacaacatc tcgctcgtga
     9661 ccgcgcacac gcggttgacc gtgtcgccga ccatcgccta ccagttctcg aaccgggtga
     9721 cggccaactt cacgctcagc tacgagaagt tcgacagcga ggacagccgc cagccctcct
     9781 cggtgaacat ccagggcaac ttcaacatcc gcgtgagcat ctcgaactga tggcggacca
     9841 ggacacgcgc cgggagacgc ttcctcgccc cgccgcagcg ggcgggcgcc agcgggtcgt
     9901 cgtgtgcgat ctcggccggg tgtcgtacgg cacggcgtgg gatctccagg cccgcctgca
     9961 ggcccggctc gtcgccgcga aacggggcga ggctcccccg gcgccgcacg tcctcctgct
    10021 cgtcgagcac ccgcccgtcg tcacgatcgg caagagtggc gatgccacac atgtcctgtt
    10081 cgacgaggag gcgctcgcgc ggcgcggcat cgggctgtac cgggtggacc ggggcgggga
    10141 cgtcacctat cacggacccg gccagctcgt cgcctacccc atcctggacc tggaccggtt
    10201 cttcacggac atccaccggt acctcaggga gctcgaggag gccgtcatcg gcgtctgctc
    10261 cgacctgggg ctcgcggcgg gccgaatcga gggacggacc ggcgtgtgga ttggtccgga
    10321 cgaccggggc ccggaacgca aggtgtgcgc gatggggatc cgttgcagcc gctgggtgac
    10381 catgcacggc ctggcgctca acgtctcgac cgatctcacc cacttcgacg tcatcgtgcc
    10441 ctgcggcatc ggcgaccggg gcgtcacgtc gctcgcgcgc gagaccgggc gcaccgtgga
    10501 ccttgcggag gcctcggaaa ggctcgtcgg ccggttcgcg gagcggttcg gggtcgacgt
    10561 cgagcggctg gcgggggagc cggcccgggc gttcctcggc gatctgctcg gcgcgccgct
    10621 ggacgcacgg gacttccggt cggcggccgc ggccttctga ccgcgcacgg ccgccccgct
    10681 tcgttcgaat ttcgggcggc atcgcccgcg ccgattcgcc cggtggtgct ccgtgtgcta
    10741 ttttgcgctc ccactcaagc gccccgacca gtcatggcca acttccatct cgaggatttt
    10801 gcgcgccggc tcatagccga gacgctcttc tatgacgagg agtacgaggc cctcggcaac
    10861 ctgagcctgg tcgatgcggc cgccacgcgt gaagccttcg tcgcgtcgta cgctccggaa
    10921 gacggcgttt tcgtgatcga ggaggccacc gagtgggagg agtacgaacc gggcgacgca
    10981 gacgacatcg ggtacgcgct ggccgtggac agccgcgagc acgggaccta cgacacgccc
    11041 gacgaggccg cggccgcgct cctggacctg gcccggaagc acaacctggc gcccagcatc
    11101 acgcttctgt tcgaggagga cgacgcaggc tgattcccca ggcggcgatt agcccccagc
    11161 ccgcgaggaa gaagacgccc ccgaacggcg tcaccgctcc cagggccgcg accccggtga
    11221 ggaccagggt gtacaggctg ccggagaaca gcacgattcc gagggcgaag caccagccgg
    11281 cggcccggag caatcggttc ccggattccg cgcccagcac gccgacgagc agcatcgcca
    11341 gggcatgcat catctgatac cggacggccg tatcaaacac gtcgagacgc tccggggaga
    11401 tcacgtcccg gagtccatgc gccccgaatg cgccggcggc cacggccagc ccggcgagca
    11461 cggagccgat cacgatgaaa ggacgtcggt ttaccatgac gatccggcgg aggaactgct
    11521 gcgcttcact actccgttcg ttgtaagtcg ctcctttcgg ggttaatttt cacgataccg
    11581 ccgactccgg ggtcccccca tggcattcac gttttccgac gcagacatcg accgcatcgc
    11641 ggccgtgctc gaggccgagc ccaagcggga cggatcactc gtccgtttcg agctcaccga
    11701 cgacgaaagc ggccgacgcc tcgccctcga gatccggact tcgctcgacc tgccccagag
    11761 agccgtggcg gactcggtgt ccaacctggt ctcggtttac gcggcgtcct cgttcctcca
    11821 gatccagggc tgcacgggct tcatcgccag ccaggaactg ggcgaggtga tcttcgtcgc
    11881 acgcagcgga cacgccgcca acggcctggt ggtcgagagg caggcgggct gctcgctcta
    11941 cgccaacgtg catccgcgcc ttctgtcggc ggatttcacc cagctgccgc ccgagctgat
    12001 catgagcagc gtggcccttt cgatggccga agacctgttc ggagacctgg gctgagcctc
    12061 gccgcatgac cgaccggccg acccgcgacg cggcgccaac cggaccggac ccgggcctcg
    12121 aggtgagctg cgtgcatccg gagcgtcacg cgccgggcgt cgatctcgaa gcgctcgcgc
    12181 gccgcgtgct cgagggagag cgctgcgcct gggtatacgt gggcatcgtg ctcgccgatc
    12241 acgcgctggt gcaccggctg aaccgcgagt acctgggtca cgacttccgg acggacgtgc
    12301 tctccttcct catagaccgt acggaccggg gcatcgaggg cgaggtctat gtcgacctcg
    12361 acacggccga atcgaacgcc gcccggttcg gcgagcccta cgagcgggag gtggcgcggt
    12421 acgtcgtcca cggactgctg cacctctccg gccacgacga ctctaccgaa gaggagcgcg
    12481 cccgcatgag gctcctcgag gatcgctacc tcggcccggt cgcggttgac tgagaccgcc
    12541 gaccggcgta ttcaccgtca gccccctgtc ccacccaatc acccgccgcc gcatgcagat
    12601 ccaggtcacg gcccggcatt tcgatgccga cgacagcctg aaggaatacg ttcagatgaa
    12661 ggtcgccagg ctccagcgtt actatgacgg catcacggac gtgcaggtcg tcctgacccg
    12721 gaacgcccag gtgcccgagg cgctcgcgga gatcgtcgtg ggcgtcttcc ggaagcagct
    12781 cacggcacgc gccgagagcg ccacccacga ggaagccatc gaccgctccg tggagcgcat
    12841 ccgccgacag ctgtaccgct acaaggcgcg cctgcgcggc acggaccggg acgagcaccg
    12901 gtaaggcacg aaggccccgg gaaggcggcg gaccgtctcc ggccggacgc accgccggcc
    12961 ctcaggcgag gcggcggagg tcctgcacca cggtccgcac gtgatccgcc gtggccatga
    13021 tgtagaagtg caggtacggc acccccgcct cgagcagccc gaccgcctgc cggcgcgccc
    13081 actcgacgcc gaccgcggcc acgtgcccgt cgggcgcctc gagcacctcc ccggccagct
    13141 cctccgggat cgagacgtgg aaccggctcg ggagcgacga gacctgccgc ttcgaggtga
    13201 ggatcttgag ccccgggacg atcggcacgg tgatgccggc ctcgcggcac ttgccgacga
    13261 acgccaggta cttgtcgttg tcgaagaaga gctgggtcgt gacgtagtgg gcgcccgcat
    13321 ccaccttctg cttcagcttc tggatgtccc aggtgaggtt gggcgattcg aagtgcttct
    13381 ccgggtaacc ggccacgccg acgcagaaat ccgtggccgc ggcatcgacc agctcctcca
    13441 ggtagcggcc ccggttcatg tccacgatct gccgcacgag gtcgaccgcg tactcgttgc
    13501 tggtccggtc ggggcggatc ggcttgccca ccccgtggtc atcgccacgg agcgccatca
    13561 cgttcctgat cccgaggtaa ttgagctcga tgagcgcatc ctcggtctcc tcgcgcgtga
    13621 aacccaggca caggatgtgc ggcacggtct cgatcccgta gcgcccgcgg atggacgcgc
    13681 acaggcccag cgtgcccggc cgcttgcgct tgatgaggcg gcgccacgag ccgtcgggct
    13741 gctcctcgta gtacgcctgc gccgagtgcg acgtgacgtc gatgaacggg ggctcgaact
    13801 ccacgagctg ctcgacgatg tccaggatgc cctgcaggct gcctccccgt ctcggaggga
    13861 tgatctcgta cgaaacgaga ggggccgaag cccgctccag cagttctgca actttcatcg
    13921 tcgaaccgga tggtgccctg gcggccggac cgctgcccgc cgccgaagct cagttctgcc
    13981 tgcgcacgac gtcttcccac caggcctcgg ggagtccgtc ggaacggccg ggtgcgtagt
    14041 cgtaccgggc atggtggaac tcccgccacg tgtcgcgacc cgaaaaccac gcaggcggcg
    14101 catcgtcccc cgccgcgacc gcgggccgga ccggcatgcg cgccagctgc acgagcaccg
    14161 ggtccagcgg ccggagcacg gagtcctcgg gacggagaat ccgcagggcc gtcatggacg
    14221 cccatacggt catcacagcg accgtcgggg cggcaacgag tacgagtaga aggctcatgg
    14281 acggggaatc agggcgtgtc cgggcggttg atgcggatgg attccggatc gggttccctg
    14341 gccttgagcg cctcttcctc gcgcgcctgg tccatgatgg tacccttcgt ggtattcggg
    14401 aggtctcccc tgaactgcgt gtcgagcagg gtgaacgcga ggaacaccac gacgaacagg
    14461 gcgcccacgg tgaggatgac catgttcacc cggttgtcgt acttcagggc catgaagaac
    14521 aggaccacga gcgtaccctt cgccgccgcg atcgcgagcg cgatgggcac gttgagcggc
    14581 ccgagatgga cgcgcgacac cgcgaccgtg acgatcgtca ggacgaccag ccccgcgaag
    14641 accgccacca gcgtgcgggt cggcgtgatg tggtgcgtgt gcccgtcagc gtgtgccatg
    14701 agcgtcagat gagatagagc agcgggaaga ggaagatcca gatgatgtcc accaggtgcc
    14761 agtagaggcc ggtgagttcg accggcgtgt accaggcgct cgagaagcgg ccgcggagcg
    14821 ccttgaccag gatccaggtg aagacgccca tgcccaccag cacgtggacg ccgtggatgc
    14881 ccgtcatcac gaagtagatc gagaagaact ggggggcgta cgggatgtcg tacagggcga
    14941 gatcatgccc gtggtattcc cccaccggcg cgaaggcctc gccgggaaag atgcccgcct
    15001 ggaatttgcc cgtgtactcg aagtacttga cgatcatgaa gaccgccgca catgccagcg
    15061 tgatcaccag gttgatgacc aggccgcgcc ggtcgtcgtg ctggatccgg ttgatgccgt
    15121 acgccaccgt gaaggacgac agcaggagca cgatcgtgtt caggccgccc agtcgccagt
    15181 ccagcaggtc ggccgaatgc tggaagacct ccggatgcca cgcgcggtac accgcgtagg
    15241 ccacgaacat cccggcgaag agcaggatct cggtgatcag gaagagccac atgcccatct
    15301 tcgcggcgtc gaactgctgg tccgacgtca cgaaatggtg ctgaaggtgc gccgggggct
    15361 cggcgtcgtg ctccagcgtg gtggccgtgg gtgttgccat ggattcgcgc gaggggtgct
    15421 cggttggggc gatcggtcgg ggttccggga tcaggcttcg gtggactctc gctcggccgg
    15481 gcgcacgggc ggcgtcgccg ttcccgcccc gtcgccggcc gggccgtcac cgaagagctc
    15541 cggcgccagg tggtagtcgt aggggccacg cgtcacgatc ggggtcgtct cgaaattgtg
    15601 cgggtgcggc ggcgacgtcg tgtgcgtcca ttcgagggtg agcgcccccc acgggttggc
    15661 cggcgcccgc ttcttcgaaa agaggctcgc caggaggtag cccaggatca ggaagagccc
    15721 ggcggagagc acccacgagc cgtacgtcga gagctcgtgc agcggctgga attccggcag
    15781 gtagtcgtag tagcggcggg gcatgccctg cgacccgagg atgaactgcg ggaagaaggt
    15841 caggttgaag cccacgaaca ccagggcggc ggcgatgcgc ccggccgtct cgttgaacat
    15901 cttgcccgtc atcttcggcc accagtagtg caggccgccc agcgccgcga tcacgttgcc
    15961 gcccatcatc acgtagtgga agtgcgcgac gacgaagtac gtgtcgtgca ggtgcacgtc
    16021 gatcgccagc acgccgagca tgatgccggt gaacccgccg atcgagaacg tgaacaggaa
    16081 cgcgagggcg tagagcatcg gcgtcctgag ccacacggat ccccggtaca tggtcccgac
    16141 ccagttcagc accttgacgc ccgtcggaat gccgatgaga tacgtgatca gcgagaacac
    16201 gatcgaactc gtcgccgact gccccgagac gaacatgtgg tggccccaga ccaggaagcc
    16261 caggaacgcg atcgcgacgg acgagagcgc cacgaacttg tagccctgca cctcgttgcg
    16321 cgagaaggtc cccatcaggt ccgagatgat cccgaacgcc ggcaggatca tgatgtacac
    16381 ggccgggtgg ctgtagaacc agaagaagtg ctggaacagc acagggtccc caccgagcgc
    16441 cgggtcgaag atgccgatgc cgagcaggcg ctcgaggaac agcaggacca tcgtgatgcc
    16501 gatcacgggc gtcgcgagca cctgcacgat gctcgtggcg tagatcgacc agacgaagag
    16561 cggcaggcgg gtccacgtca ggcccggcgc ccgcatcttg tggatggtga ctatgaagtt
    16621 cagacccgtc aggatggacg agaatcccat cacgaacact cccagcgtca cgaacagtat
    16681 cgagtcgccc acccgctccg agtacggcgc gtagaaggtc catccgccgt cgatccggcc
    16741 caccaggagc gcgctcatca cgatgagggc gcccaggatg tagatgtacc agctcgcgag
    16801 gttcaggcgc gggaaggcca cgtccttcgc gccgagctgc atgggcagca cgaagttgcc
    16861 catcaccgcc ggcacggccg ggatgatgaa ggcgaagatc atcaggacgg cgtgcagcgt
    16921 gaacgactgg ttgtaggcgt ccggccccat gatcgtctcg ccaggggcca gcaactccac
    16981 gcgcacgagc agggccagga tgccccccga gaggaacgcc agcgaaacgg ccgccaggta
    17041 catcaggccg atccgcttgt ggtccagcgt caggagccac gatttcagcc cccgttcgtg
    17101 gttcaggtaa ttcaccgcgc cgtgaatggg cgcggacggg ttcgactcgt tcatgactga
    17161 tgccattcct tgggaagcga tgcgggcacg ggcggcctat tccagggact ggatgaaggc
    17221 gatcaggccg tcgagctgct tggccgacag cgagccgaag gtcgcgggca tcgcgttcgg
    17281 gtacgtctcg acgatcctgg cgccgggggc gaggatcgac tcccggatgt agttctcgtc
    17341 ggccaccgcg gtcgtgccgt cctcgaagcg ccgctccgcc ccgtacagtc ccttcaatga
    17401 gggtccgacg atgcgcgtgc cgtcgaccga gtggcagacg ttgcagttgt actgccggaa
    17461 caggtcctgc ccgagctcga gcggcggccg gtcggcgccc tgcgcggcga gccaggcctg
    17521 gaagccctcc tccgtctcca cgaccacctt cccgagcatg ccggagtgcg aggtcccgca
    17581 gtactcggtg cagaatatct ggtactcacc gatctcggtc ggctcgaacc agagcgacgt
    17641 gtagcggttc ggcagaacgt cctgcttcac gcggaactcg ggcacgaaga agctgtgcag
    17701 cacgtcctcg ctcgacatgt tcagcttcac gggcacccct atggggacgt gcatctcgtt
    17761 ggtcgtcacc gtcccgttcg ggtactcgaa ttcccagaac cactgcttgc cccgcaccgt
    17821 gatctggtac gagtcgggcg gcggggaata caggtggacg tagaccttga atccccaggt
    17881 gaacacgatg agcaccagga tcgtcggcag gacgacccac gccacctcga gcagccggct
    17941 ctcgtgcacg ggagcgggca cctcgtgcgc attccgccgt cggtagcgga aggcgaaata
    18001 caccatcgcc gccatgacca tggcgaagat gatgagactg gtccagaaca cgaagtagaa
    18061 cagggcgtcg acctccggcg ccaccgtcga cctcgcgtcg ggcatccaaa cgtttccgtt
    18121 ctctcccatg gtgtgtgttg gtggtgtcgg gcctcaggcg gccccggcgg gacggagcgg
    18181 gccgtgctcc ctccgccaca ggagggccat gcccacgacg aggagcaaca gggtgagcag
    18241 accgcccagc ttcatcaggt tgcgggcgtg aaggacgtac gagttggcgt ccgcgtcgta
    18301 ccggtagcag tagagcagga tgcgatcgac cgcgctcccg acccggccct cggaggcctc
    18361 gacgagcgcc ttgcgcacgt cggccggcgg atagcccatg ccgtggatgt agcgggtgat
    18421 cattccgtcg ccggaggcga agatgagcgc ggcggggtgg gcgaactccc gggtcgactc
    18481 cacccagcgg aaccggaagc ccagcgcgtc ggcgagcacc tggatcgact cgtgctcgcc
    18541 cgtcaggaag tgccacccgg cgccggcctc cggccgcccg agctgggcca ggtacttttc
    18601 cttggcacgc gccgcgatcg tgggggcctc gttcgcggag aagcttacgg tgaccacatc
    18661 gaactcgccg cccggctgcc actccatctc cgacagggtc cgcgtgaact ccgtaaggag
    18721 caggctgcag agcatcgggc actcgtggta cacgaggttc aggacgaccg gccgctctcc
    18781 gtcgaagtac tcgccgagcg cgaccgtgcg cccggtctcg tcctcgaacc gggcgtcgag
    18841 cggcagccgg tcgcccagcc gctcgtcgat gcccacctcg tcgaagacgg acgggatgga
    18901 acccgccggc tgcgccgcgg acagggaagc gccggaaacc gtcccccagg ccaggaggac
    18961 gccgaaaagg gctccgaatc gagtcatcgg gtggtcggcg ggacgtggtt caacgggtga
    19021 ggcggacctc ttccgagacc ggccggtctc cctcggccgc cgcctcgttg accatgaggt
    19081 caatcgcgcg atccacggga atccggtaac gccgcgcggc ggcgtcgacg acgccgtact
    19141 gcgtcagctt ctcctcgccc gcgatcctcg tctcgacgag cgcgggatag ccggtcaacg
    19201 ccacggcctc accctcggcc gccctgaact cggacctggc gagctgcacg gcgatgagca
    19261 cgaggacggc gaggatcagc ccgccggcga ccgtcaggcc caggatgcca aggcccgaga
    19321 caccctccgg ctcgaagccg gagtcggcgt gctcatcgtg taccggtacg gaagtcatct
    19381 tcgcgcgcgt agcgtcgatc tgcggggttc cgggatccgg atgccgccgc ccgacgggga
    19441 tcgatcggat gcggcgctcc aagaaagaca aagataccaa acaggaggcc ggccgttcca
    19501 cgaccttttc cctgacccgc ctgctgcgta cacccgtgtg agggcgctgc catcacgagt
    19561 tcaggaaacc gagcgactgg gcaagtcgcg gatcccgctg cggtaccatg ctgtggcggc
    19621 tcatccggaa gatgagcacg gccacccaga tgccgccgag gcccaggagc gccgtgaagt
    19681 cgagcaggtg gaagccggcc tcctcgtgca ggacgggcat cacgatccag tgcaggtcga
    19741 accagtgcat gaacaggagc cacaccgaca tgaaggcgag caggccgagc atgcgcttcg
    19801 cccaccggga tatcagcacg acgaacggga tcgcgaaatg gaagatgagc agcgtcaccg
    19861 agtgcagttc ccatccgtgc tcgagccggt gccggtacca gacggtctcc tcgggcaggt
    19921 tgccgtacca gatcagcatg tactggctga acgcgatgta cgcccagaac accacgaagc
    19981 cgaacatgaa cttgcccaga tcctggtaat gctcggccga aacgccctcg cgcagcatcc
    20041 cgccgcgctg gtacaccagg gcgagcagga tcatgaaagc catgatgccc aggaacgagc
    20101 ccccgaagaa gtacacgccg aagatcgtcg agaaccagtg cgggtccagc gacatcagca
    20161 ggtcgaagct ggcgaacgcc gtggtcac
//
LOCUS       DBNHQZ010000073         6597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1104306, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000073 DBNHQZ010000000
VERSION     DBNHQZ010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1104306"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="AC16YU_01875"
     CDS             <1..567
                     /locus_tag="AC16YU_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6063998.1"
                     /translation="QGDSVDLSVNAADELRIHNPGSARTYGLTVRMESASGVVSRHQE
                     SIVLGDGATHEVVPDWSNPAVPVTVHVDDDSDGTFEGSFELADSGTGMRGVPGEAVPQ
                     RFGLRSVHPNPATDRVTLGIDLPRPARIRITVTDILGRVVTRPWSETRPAGAYELALD
                     LPDLGNGVYLIHLDAGVRVSTKSLVVLR"
     gene            732..804
                     /locus_tag="AC16YU_01880"
     tRNA            732..804
                     /locus_tag="AC16YU_01880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:765..767,aa:Gln,seq:ctg)
     gene            1151..1276
                     /locus_tag="AC16YU_01885"
     CDS             1151..1276
                     /locus_tag="AC16YU_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6063999.1"
                     /translation="MGDKSPKSTQKKSSQKQVKANKSERKKKDAEEARRTALPRK"
     gene            1514..3898
                     /locus_tag="AC16YU_01890"
     CDS             1514..3898
                     /locus_tag="AC16YU_01890"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR6064000.1"
                     /translation="MDTLAVAFDGMTGQVEVGGPYAGAEFHDSRPVPARISFYYPVAN
                     SIDLSTDYWRRGESRPLTLVVRVDGRPDTLGNRPWGYRWTPYSVVFREQAPGYDAALE
                     YRFADDLPLVVMRLTVWNRSGDRAAFGIDARLATSLRTSHRYATRDPASTRYVDGGSA
                     FLASFDAVDTDSVELFVANVGASPDPVSRPSGSAAEFTYTRDLEPGDSLVVTQLIGTS
                     RQRESDALRKRAVGQWAGEVAAYEDGIRAYVRDQGRFELQDPELRQTDRLARALLRAN
                     RHHLDGHIVPMPSPAEYNFFFTHDLLLTDLGAVFFDVARVREDLHYVQSLVRADSVLP
                     HARYWRDSTYVTEPANADNWNHLWAVLLAASYLKHSADTATVGALYPTLQKSIALMLG
                     NEHGGLMYAERPDWWDIGHVYGPRAYLTALTIRALDAYAFLTIQLGRDGAELAVHAET
                     ADRMRRALVDVLWDEDAGYLLNGLDSTTLDRHYYTGSLLAAAYDLLDPPKRDALLETA
                     RRELLDELVGMRNAMPADYHLLRDVYHFQEGEVGGPFLYMNGGVWPQGNAWYALGLIA
                     AGRVDDARAVLERYLSVAGVLDSPNGQPAFYEYRNADPASPVYGAVDKPTFLWAGGWY
                     LHVLYQLAGLRETPWNLSFRPELPEGFETVAYDLAVEGRQSRVTWSGAGSTFRRIAVD
                     GVESASAVLTGPASRIELERGVPAAPYLAEASCIVDRVWRERGGRSLAVGVRGVVGQR
                     VVLEVIAPTRLSDIRVNGEAVHADSMAAEESDGVAVMRVFWTLETTSAVVTFGP"
     gene            3977..5077
                     /locus_tag="AC16YU_01895"
     CDS             3977..5077
                     /locus_tag="AC16YU_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6064001.1"
                     /translation="MRHHGIGIVGYGGFGRFLHETWGGMDRVSVEAVADIGFAGAPDV
                     TSYTDWKDLLTHPGIDLVAIVTPPAQHAEMACAALAAGKHVIVEKPIATTRKDAQRVL
                     DARNRSGRVAAVDFMLRFNPVVEAVHAWCGNRAFGELRRVLVENYAQDESLPPGHWFW
                     SREDSGGILVEHAVHFIDIVQGCRPGRATQVFGALQRRNPGQADRMLATVIHEGGLTE
                     SHYHAFSRPRAFERTSMRFVFDRAEVDVRGWIPMAGEVTALVSVDSESELDRLPGFRV
                     THRQALSQVDETGGRSSHASTPVRGMQTLVEGTFAMSVTKLEAYAAALRGLMNDVVDG
                     MEHPGHVLRVTLEDAMIGLEIALHATEAAYRG"
     gene            5188..6225
                     /locus_tag="AC16YU_01900"
     CDS             5188..6225
                     /locus_tag="AC16YU_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6064002.1"
                     /translation="MAISKRTTIKDVASEAGVAISTVSLVMNGKGYVSRDMRVRVRKA
                     AQLLGYVPRHAARSLPSRRTGNVGFVVREDHFQRTESFYTRIYLGTEFHANHHRFYVL
                     LTTIPKTYVSGKDTPRLLKELNVDGILIAGKVDDAFIGDAQGSGVPVVLVDFEHDGLP
                     AAVIDNLGGARSAARHLIERGHERITFLGADMRHPSLSARREGFQQACAEAGLPADQL
                     RFLIADDGEPDYQTGARLCKRLFEDGDRPTAVFCINDAMALSVLDHAAQLGLGVPEDV
                     AVVGFDDVPAAERSQPALTTIRVPNELLGELAMRYLLDLVHDTPSIETPYVRGPHTIR
                     VPTELIIRNTT"
     gene            6324..>6597
                     /locus_tag="AC16YU_01905"
     CDS             6324..>6597
                     /locus_tag="AC16YU_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064003.1"
                     /translation="MTNSLRKKLAWAGAVLAAILVLLPVPTRAQDALSRIVAANSAAN
                     LLSNGGFETMKPAYWEPSGAGATWSREQSRTPEWSLQLEGPGANWVQ"
BASE COUNT         1130 a         2148 c         2198 g         1121 t
ORIGIN      
        1 cagggagact ccgtcgacct gtcggtgaat gccgccgatg aactccgcat ccacaatccc
       61 ggcagcgccc ggacgtacgg cctcacggtg cggatggagt cggcatcggg cgtcgtgagc
      121 cgccaccagg agagcatcgt tctcggcgac ggcgcgacgc acgaggtcgt ccccgactgg
      181 tcgaatccgg ccgttcccgt gaccgtgcac gtcgacgacg acagcgacgg cacgttcgag
      241 ggctccttcg agcttgccga ttccggtacc ggcatgcggg gcgttcccgg cgaggccgtg
      301 ccgcagcgtt tcgggctgcg gagcgtccat ccgaatcccg cgaccgacag ggtcacgctc
      361 ggtatcgatc tcccgcggcc ggcccggatc cggatcaccg tcacggacat cctcggacgc
      421 gtggtgacga ggccgtggtc ggaaaccaga cctgctggcg cgtacgagct tgccctcgac
      481 ctgcccgacc tgggcaacgg ggtctacctg attcatctcg acgccggagt gcgggtctcg
      541 acgaagagcc tggtcgtcct gcgctagcta ccgggaagct cctcaccggc gcggcaagcc
      601 ctcgaaaaag cctcgaagcc ggcggcggag cagttcaatg ggctaaattc ggaacttgcc
      661 gggaacagcg gcgctccctg ccggtgtcac cgcgccttcg gccggccgct cgcggccgcg
      721 gcccgcgaca ttgccctata gctcaattgg cagagcgcct gactctggat caggaggttc
      781 caggttcgaa tcctggtagg gcaacatgac gacgcctcgg ggctatccgg tcccgaggcg
      841 ttgttacatt ctgtgaggag tcggacactg ccaaggttcg acatgaggcc ggcgattcag
      901 ccggcatgtt cacctcgccc gttcgtgccg gccgattgat ggagcgagcg aagccccgaa
      961 taatcctggt agggcaacat gaccgcgcct cggggccacc cggtcccgag gcatccccac
     1021 gctttactgg gattcgtgtt gagcccaggc agtgggcaac cacgatggcc acgaaacgca
     1081 ccgggtaacc tggacgcgct taccttgacg gcacagaccg gatgccgaac tccaaccgag
     1141 gttgatgacc atgggtgaca agtctcccaa gtccacgcag aagaagtcca gccagaagca
     1201 ggtcaaggcg aacaaaagcg agcggaagaa gaaggacgcc gaagaggcca gacgcactgc
     1261 tttgccgagg aaatagttgc gtccgtgccg cagaacccgc caccgccgac cgatggaggg
     1321 cgtgacccct attgcgtgcg tgaactcacc cgccgcctct gaggatggac ggttgctcca
     1381 ggagcggcag gacggaagcg atgcgcactt ctgttccgcg cacaggcggt cgcatggcac
     1441 agtctcgaac gcgtgcgggc tgctctgtct gctggtagcg ctggcccctc cggcccaggc
     1501 ccagaaccgt ctcgtggaca cgctcgccgt tgcgttcgac gggatgacgg gacaggtgga
     1561 ggtcggggga ccctacgcgg gcgccgagtt ccacgacagc cgacccgttc ccgcacggat
     1621 cagcttctat tacccggtgg cgaacagcat cgacctgagc acggactact ggcgccgcgg
     1681 ggagtcgcga cctctgaccc tcgtcgtccg ggtggacggc cgtccggaca cgctcggaaa
     1741 caggccctgg ggctaccgct ggacgcccta cagcgtcgtg ttccgcgagc aggcgccggg
     1801 ctacgacgct gcgctcgagt accgtttcgc ggacgacctc ccgttggtcg tgatgcggct
     1861 gacggtatgg aaccggtcgg gcgaccgcgc cgccttcggg atcgacgcgc gcctcgcgac
     1921 ttcccttcga acgtcacaca gatacgcgac acgggacccg gccagcactc gatacgtgga
     1981 tggcggctct gccttcctcg cctcgttcga cgccgtggac acggattccg tggaactctt
     2041 cgtggccaac gtgggggcct ctccggaccc cgtgagccgg ccctccggct ctgcggccga
     2101 gttcacgtac acgagagacc tggagcccgg cgactcgctc gtggtcacgc agctcatcgg
     2161 aacgagtcgg cagcgggaga gcgacgcgct ccggaagcgg gccgtgggtc aatgggccgg
     2221 tgaggtcgcc gcctacgagg acggcatacg ggcgtacgtc cgggaccagg gccgcttcga
     2281 gcttcaggac ccggagctgc gacagaccga tcgccttgcg cgggcgctgc tccgcgccaa
     2341 ccgtcaccac ctcgacggtc acatcgtgcc gatgcccagc ccggccgagt acaacttctt
     2401 ctttactcac gacctccttc tcacggacct cggcgccgtc ttcttcgacg tggcgcgtgt
     2461 tcgcgaggac ctgcactacg ttcagtccct cgtccgcgcc gactcggtgc tcccacacgc
     2521 ccgctactgg cgcgacagca cctatgtcac ggaacccgcc aacgcggaca actggaacca
     2581 cctgtgggcc gtcctgctcg cggcgtctta cctcaagcac agcgccgaca cggccaccgt
     2641 cggtgcgttg taccccaccc tgcagaagag catcgcgctg atgctcggca atgagcacgg
     2701 cgggttgatg tacgccgagc ggccggactg gtgggacatc ggccacgtgt acggtccgcg
     2761 ggcgtatctc actgcactca cgatccgtgc gctggacgcc tacgcgttcc tcacgattca
     2821 gctggggagg gacggcgcgg agctcgccgt ccacgccgag acggcggacc ggatgcgccg
     2881 cgctctcgtg gatgtcctgt gggacgagga cgcggggtac ctcctgaacg gactcgactc
     2941 gacgacgctc gatcggcact actacaccgg gtcgttgctc gcggcggcat acgacctgct
     3001 cgatccacca aagcgcgacg cgcttctgga gacggcccgg agggagctgc tcgacgagct
     3061 cgtcggcatg cgcaacgcca tgcctgcgga ctaccacctg ctccgggacg tctatcattt
     3121 ccaggaggga gaggtcggag gtccattcct gtacatgaac gggggcgtat ggccgcaggg
     3181 gaatgcgtgg tacgcgctgg gactgatcgc cgccgggcgt gtggacgacg cccgggcggt
     3241 gctggagagg tacctcagcg tagccggcgt cctggacagt ccgaacggtc aaccggcctt
     3301 ttacgagtat cgcaatgccg atcccgcgtc ccccgtctat ggagccgtgg acaagcccac
     3361 gttcctgtgg gcgggaggat ggtatctgca cgtgctctac cagctcgccg ggttgcgtga
     3421 gacaccgtgg aacctctctt tccgcccgga gctgccggag gggttcgaga cggttgccta
     3481 tgacctcgcc gtggagggcc ggcagagccg cgtgacgtgg tctggtgccg ggtccacgtt
     3541 ccgccgcatc gccgttgacg gcgtggagtc cgcatctgcc gtcctgacgg gacccgcatc
     3601 gcggatcgag ttggagcgcg gggtgcctgc ggctccgtac ctcgccgagg cttcctgcat
     3661 cgtcgatcgg gtatggcgtg agaggggcgg ccgttcgctg gccgtcggtg tccgcggggt
     3721 cgtcgggcaa cgggtcgtcc tggaagtgat cgccccgacc cggctgtccg atatccgcgt
     3781 gaacggggag gccgtgcacg cggactcgat ggcggcggag gagtcggacg gagtggccgt
     3841 aatgcgcgtc ttctggacct tggagacgac gagcgccgtc gtaacgttcg gaccgtgagg
     3901 tccagggctc gcggaacaga cccaccggcg ccggtccgac ggccggcatg cagcaacctt
     3961 cgaaatggtg cgcggcatga gacaccatgg aatcggcatt gtcggctatg gcggcttcgg
     4021 acgcttcctt cacgagacct ggggcggaat ggaccgggta agtgtcgagg cggtcgccga
     4081 catcgggttc gccggtgccc cggacgtcac gtcgtacacc gactggaagg acctgctgac
     4141 ccatccgggc atcgacctcg tggcgattgt cacacctccc gcgcagcatg ctgagatggc
     4201 gtgtgccgcg ttggccgcgg gcaagcatgt gatcgtggag aagccgatcg caaccacacg
     4261 gaaggatgcc cagcgggtgc tggacgcccg taaccgctcc ggacgcgtcg cggccgtcga
     4321 tttcatgctc cggttcaatc cggtggtgga ggccgtccac gcctggtgcg gcaacagggc
     4381 cttcggcgaa ctgcgcagag tcctggtgga gaactacgca caggacgaga gcctgccccc
     4441 gggccactgg ttctggtcgc gcgaggattc agggggcatc ctggtcgaac acgcggttca
     4501 tttcatcgat atcgtgcagg gctgcaggcc gggacgagcc acgcaggtct tcggcgcgct
     4561 ccagcggcgg aatcccggcc aggcggaccg gatgttggcc acggtcatcc acgaaggcgg
     4621 gctcacggaa tcccattacc atgccttctc tcggcccaga gcgttcgagc gaacgtccat
     4681 gcgttttgtc ttcgaccggg ccgaagtcga cgtccgggga tggatcccga tggccggcga
     4741 ggtgacggcc ctcgtcagcg tcgactccga atccgagctg gacaggctcc ccggattccg
     4801 ggtgacgcac cggcaggctt tgtcgcaggt cgatgagacc gggggccgat cgagccatgc
     4861 ttctaccccg gtccgcggga tgcagaccct ggtggagggg actttcgcca tgtccgtgac
     4921 caagctcgag gcctacgcgg ctgcactgcg cggcttgatg aacgacgtgg tggacgggat
     4981 ggagcatccc gggcacgtgc ttcgggtcac actcgaggac gcgatgatcg gcctcgagat
     5041 cgcgctccat gcgacggagg cagcataccg gggctgactc cccggcggga ttggacgtcg
     5101 cgggcgaccg aaggggcttg acattctgga tatttcgtat agtttctcga aacgtttcga
     5161 gaaccccatc cagaccgatc aaaccccttg gcgatttcca agcggacgac gataaaagac
     5221 gtcgcgtcag aggctggcgt ggcgatatcg actgtctcgc tggtcatgaa cggcaagggg
     5281 tacgtgagcc gcgatatgcg cgttcgtgtg cgaaaggctg cacaactgct cggttatgtt
     5341 ccccggcatg ccgctcgctc gcttccgtcg cgaaggacgg gcaacgtcgg cttcgtggtc
     5401 agggaagatc acttccagag gaccgagtcg ttttacacgc gcatctacct cggcacggag
     5461 ttccatgcca atcaccaccg tttctacgtc cttctgacca ccattccgaa aacctatgtg
     5521 agcggcaagg acactccgcg tcttctgaag gaactgaacg tggacggcat tctgattgcc
     5581 ggaaaggtgg atgacgcgtt catcggggac gcccaggggt cgggagtccc cgtggttctt
     5641 gtcgatttcg agcacgacgg cctcccggct gccgtgatcg acaatctggg tggtgcacgc
     5701 tcggctgcgc gtcacctcat tgagcgtgga cacgaacgga tcactttcct gggggccgac
     5761 atgcggcacc ccagcctgag cgcgaggcgc gaaggattcc agcaggcctg cgccgaggcc
     5821 ggactcccgg ccgaccagct tcgattcctc atcgcggatg acggtgagcc ggactaccag
     5881 acgggcgcaa gactctgcaa gagactcttc gaagacggcg accggccgac ggccgtcttc
     5941 tgcatcaacg acgccatggc cctgagtgtc ctggatcacg ctgcgcagct cgggctgggt
     6001 gtgccggagg acgtggccgt ggtcgggttc gacgacgtgc cggcggccga gcgctcgcaa
     6061 ccggcgctca ccacgattcg ggttccgaac gagcttctcg gcgagctcgc catgcggtat
     6121 ctgctcgacc ttgttcacga taccccttcc atcgagactc cgtacgtgcg tggcccgcac
     6181 acgatcaggg tcccgacgga gttgatcatc aggaatacaa cgtgacctgt ccccaggatg
     6241 gtgaacctcc gaccacatac cggaagacca tctccaccca ctgggccgcg ccgacgcccg
     6301 gcccgcatta tcggagaacc gtcatgacga actccctacg aaagaaactg gcctgggccg
     6361 gagcggtgct cgccgcgatt ctggttctgt tgcccgttcc gacacgtgcc caggatgccc
     6421 tgtcacgcat cgttgcggcc aactcggcag cgaacctgct gtcgaacggt ggattcgaga
     6481 cgatgaagcc ggcctactgg gagccctccg gtgccggtgc gacgtggtcg cgcgagcagt
     6541 cgcgcacgcc ggaatggagc ctccagctcg aaggccccgg agcgaactgg gtccagg
//
LOCUS       DBNHQZ010000074         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3408331, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000074 DBNHQZ010000000
VERSION     DBNHQZ010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3408331"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..747
                     /gene="rpsD"
                     /locus_tag="AC16YU_01910"
     CDS             145..747
                     /gene="rpsD"
                     /locus_tag="AC16YU_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6064004.1"
                     /translation="MARDRGPKQKIARRFKEPIFGPSKSLERKPHPPGQHGRGRRSKE
                     SEYAVQLKEKQKAKYTYGLLERQFRNLFDKAARKKGVTGENLLKFLEARLDNTVFRMG
                     FARTRRQARQLVTHGHVTVNSQVVDIPSAQLRAGDIVALRPKSRNLYAVRDAVKTTRR
                     SFPWIEVDRNLMQGKFLDYPNRSDIPENIREQLIVELYSK"
     gene            833..1810
                     /locus_tag="AC16YU_01915"
     CDS             833..1810
                     /locus_tag="AC16YU_01915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6064005.1"
                     /translation="MSTYTIQMPDGVEVEEASGNYGRFVIQPLERGYGVTIGNALRRA
                     LLSSLPGVAITAVKIDGVQHEFSTIEGVTEDVSDIILNLKGVRFQAGEAAEGVIHLVV
                     KGPGEWTAADIAGATSEYEVLNPDHHIAILAKGSNLSVELRMDAGRGYVPADENKRAD
                     DPIGVIAMDSIFTPIKNVRYTIKETRVGEKIDYERLTLEVTTDGSVQPEDALAQAAGI
                     LRDHVNLFIRMDSEPQPSAEEKEVDAEVQRVRELLAQSVDELDLSVRAHNCLKAANIK
                     TIGDLVRREESEMLKFRNFGRKSLQELLQVLEERSLHFGMDVDKYLEEV"
     gene            1859..2533
                     /gene="rplQ"
                     /locus_tag="AC16YU_01920"
                     /pseudo
     CDS             1859..2533
                     /gene="rplQ"
                     /locus_tag="AC16YU_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
BASE COUNT          544 a          908 c          951 g          364 t
ORIGIN      
        1 cgtcactccc attcctcaca acggctgccg gccgcccaag cgtcgccgcg tctgatcgcg
       61 gaaagcaggg catgagtccg gctacgggga cgcacggtgc gtgcccgtac gtttgttaaa
      121 caaggcaaac gagaaagaac cgaactggcc cgagacagag gccccaagca gaagatcgcg
      181 cgacgcttca aggagccgat cttcggaccc agcaagtcgc tggaacgcaa gccccatcct
      241 cccgggcagc acggccgtgg acgccgctcc aaggagagcg aatacgccgt gcagctcaag
      301 gagaagcaga aggcgaagta cacctacgga ctgctcgagc ggcaattccg taatctgttc
      361 gacaaggcgg cccgcaagaa gggcgtgacc ggcgaaaacc tcctgaagtt tctggaggcg
      421 aggctggaca acaccgtgtt ccgcatgggc tttgcccgta cgcggaggca ggcccggcag
      481 ctggtcacgc acggtcacgt cacggtgaac agccaggtcg tcgacatccc gtcggcacag
      541 ctgcgcgccg gggacatcgt cgcgctccgg ccgaagagcc ggaacctgta cgcggtccgc
      601 gacgccgtga agaccacgcg ccggtccttc ccgtggatcg aggtggaccg caatctcatg
      661 cagggcaagt tcctggacta cccgaaccgt tcggacatcc cggagaacat ccgggagcag
      721 ctcatcgtcg agctgtattc gaagtagtcc gggctcgccc gccctcgccc cgggagcgcg
      781 ccatccgccg cgcggtgccg ggagctgcac agaacgtact ctacgagccg atatgagcac
      841 ttacacgatc cagatgcccg acggcgtgga ggtcgaggag gcctccggga actacggccg
      901 tttcgtcatt cagcccctcg agcgcggtta cggcgtcacc atcggcaacg cgctgcgccg
      961 cgcgctgctc tcgtctcttc cgggcgtggc gatcacggcc gtgaagatcg atggcgtcca
     1021 gcacgagttc tccaccattg aaggggtgac cgaggacgtc tcggacatca tcctgaacct
     1081 gaagggcgtc cgcttccagg cgggtgaggc cgcggagggc gtgatccacc tggtagtgaa
     1141 ggggcccggt gagtggacgg ccgcggacat cgcgggcgcg accagcgaat acgaggtgct
     1201 gaatcccgat caccacatcg cgatcctggc caagggatcc aacctgtcgg tcgagctccg
     1261 catggatgcc ggtcgcgggt acgtgccggc cgacgagaac aagcgggccg acgatccgat
     1321 cggcgtgatc gcgatggact cgatcttcac gccgatcaag aacgtccgct acacgatcaa
     1381 ggagacgcgc gtcggcgaga agatcgacta cgagcggctc acgctggagg tgacgacgga
     1441 cggctcggtc cagccggagg atgcactcgc tcaggcggcg ggcatcctgc gggaccacgt
     1501 gaacctgttc atccggatgg actcggagcc gcagccatcg gccgaggaga aggaagtgga
     1561 cgcggaggtt cagcgggtgc gcgagctcct ggcccagtcc gtcgacgagc tcgacctctc
     1621 ggtgcgcgcg cacaactgcc tgaaggcagc caacatcaag acgatcggcg atctcgttcg
     1681 ccgcgaggag tcggagatgc tcaagttccg caacttcggt cgcaagtccc tgcaggagct
     1741 gctgcaggtg ctcgaagagc ggagcctgca cttcggcatg gacgtggaca aatacctcga
     1801 ggaggtctag gccccagggc gccttcaccc ctccacaggt cgcgaacgaa cgacggaaat
     1861 gagacaccag aacaagggat tcaagctcgg acgcacgagc acccaccgca aggccacgct
     1921 ggccgcgctg tccaacgccc tgatccgcca caagcggatc cagaccacgt tgacgcgcgc
     1981 ccgcgcgctg cgcatgtaca tagagcccat catcacgcgg gcgaaggccg acagcacgca
     2041 caaccgccgc atggtcttcc gcgacctgca ggacaagcac tccgtgaccg agcttttcgg
     2101 cgacatcgcc ggcaaggtcg gggagcgccc gggcggatac gtgcgcatcg tcaagctcgg
     2161 tcagcgcgcg ggtgactcgg ccgagatggc cgtgatcgag ctcgtggact acaacgacgt
     2221 gagcccggac ggggcggcgt cgggccgcaa gcgccggacg cgccgcggcg cctcgggtcg
     2281 tgggaagacc gccaaggccg tcggggccgg agcggcgggc gttacggccg ccgccgaggc
     2341 cgaggaggcc gtggccgatg ccgaggaggc cgtagccgag gcaggcgagg acgttgccga
     2401 agccgccgcc gaggaggccg tcatcgaagc aggcgaggac gtcgccgaag ccaccgccga
     2461 tgccgaggag gccgtggccg aggcaggcga ggacgttgcc gaagccgccg ccgagaccga
     2521 agaggacgtc gccgaagccg ccgccgaggc cgaagaggac gtcgtcgacg agaaggctcc
     2581 cgatgccgag gccgccgcca aggcttcggc cgccgccgaa gatatcgagg cgccggcacc
     2641 ggaagccgag gacgacgagg agaagaagga ctgacccggc cggcccggat cgtacggggc
     2701 gttcccacgc gggggcgccc cgttcatttt gccccgggcg ggcattctcg ccgtttcggc
     2761 gcccccg
//
LOCUS       DBNHQZ010000075         3431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2053590, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000075 DBNHQZ010000000
VERSION     DBNHQZ010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2053590"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16YU_01925"
     CDS             complement(<1..431)
                     /locus_tag="AC16YU_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR6064006.1"
                     /translation="MPAADTIAAIATAQGAAALSVVRVSGAGSIAVADVCFRGRSRLS
                     DADGYTAHFGRIVHRGDEVDQVVATVFRSPHSYTGEDSVELSCHGGGIAARLVLEALL
                     AAGARMARPGEFTERAFVNGKMDLVQAEAVADLIHATSEAA"
     gene            complement(443..2239)
                     /gene="yidC"
                     /locus_tag="AC16YU_01930"
     CDS             complement(443..2239)
                     /gene="yidC"
                     /locus_tag="AC16YU_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR6064007.1"
                     /translation="MDRNVVIATVLIAIIMVVWLTWLTPPAPPPGTDADSTVAGTEDV
                     LRDTLPDPAADIGPEAGAPPALSPDDSTVVGASTGVERTITVTSDLYEAEFSTLGATI
                     RSWSLAEYQVADSSATVQLVDSTVAGAVSLVFTSPSSRVYDTRAFYFETDAEADRIVV
                     DDRERTLSFRAPVADGALEVVYTFAPGTYEVVMDVRMERAESFVTTSGYEVVWNGSIP
                     FTEENHENEGQHSGVYARSGGEVEKVEVTSQSYDERSLRGQVDWVAVKSKYFVAAVIP
                     SGATRGAELIGERFGEAADPAVRYGYSASLAMAVPGPDGDRYRMYVGPMEYYRIREYG
                     LGLYDMVDYGWDFFEAVTRPLAKYVFIPTFVFLAKWLPNYGLVIIIFSILIKLLVHPL
                     TKSSFTNMARTRELQPRMEAIKEKYSDNPQKQQEAMMKMYKETGVNPLGGCLPMLLQY
                     PIIIALWQFLPQAIEIRQQGFLWASDLSAPDVILHLPFSIPLYGNFVAGFTLLMGLSM
                     VIQMKLQATPSSNAQAKIFMYVFPVMIFVIFNKLASGLNLYYLCYNVLSAGHQKLINR
                     RLEAKKAAGESLFDKKEPKAGRNGQGPKRRKR"
     gene            complement(2255..2665)
                     /locus_tag="AC16YU_01935"
     CDS             complement(2255..2665)
                     /locus_tag="AC16YU_01935"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6064008.1"
                     /translation="MMRLNRSHRLKRRRLIRPLFDRSRSDVHGASVGCVRVMYRHVAA
                     DDLPRGVPIQAGFAVARGTGSAVVRNRIRRIMREAYRTGVGPLVDVVGTGGLRLTALF
                     MFRGQASLAPDCIPRDLPHLLNLVAHRLAGGTGR"
     gene            complement(2704..2859)
                     /gene="rpmH"
                     /locus_tag="AC16YU_01940"
     CDS             complement(2704..2859)
                     /gene="rpmH"
                     /locus_tag="AC16YU_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6064009.1"
                     /translation="MQPTYRPSRRKRANKHGFRSRMATRNGRRVLSRRRSKGRKVLSV
                     SDTRSRK"
     gene            3021..3093
                     /locus_tag="AC16YU_01945"
     tRNA            3021..3093
                     /locus_tag="AC16YU_01945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3054..3056,aa:Gly,seq:ccc)
     gene            3192..3268
                     /locus_tag="AC16YU_01950"
     tRNA            3192..3268
                     /locus_tag="AC16YU_01950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3226..3228,aa:Val,seq:tac)
BASE COUNT          557 a         1155 c         1138 g          581 t
ORIGIN      
        1 tgcgcggctt cggacgtggc gtggatcagg tcggcgacgg cctccgcctg gaccaggtcc
       61 atcttgccgt tgacgaacgc gcgctccgtg aattcgccgg gacgcgccat gcgggcgccg
      121 gcggcgagca gcgcctcaag gaccagccgt gcagcgatgc ccccaccgtg gcacgagagc
      181 tcgacggagt cctcgccggt gtacgagtgg ggggagcgga agacggtggc caccacctgg
      241 tccacctcgt cgccccggtg cacgatccgc ccgaagtgcg ccgtgtatcc gtcggcgtcg
      301 gacagccggc tgcgcccgcg gaagcagacg tcggccacgg cgatcgaccc ggccccggag
      361 acgcgcacca cggagagggc ggcggcgccc tgcgccgtcg cgatcgcggc gatcgtgtcg
      421 gcggccggga tccccacgtc gcttaccgct tcctgcgctt cggtccctgc ccgttcctcc
      481 cggccttggg ctccttcttg tcgaacaggc tctcaccggc cgccttcttc gcttccagtc
      541 gccggttgat gagcttctgg tgcccggccg acagcacgtt gtagcacagg tagtacaggt
      601 tcagccccga cgccagcttg ttgaagatga cgaagatcat cacggggaac acgtacatga
      661 agatcttcgc ctgcgcgttg ctgctcgggg tggcctggag cttcatctgg atgaccatcg
      721 agaggcccat gagcagcgtg aagccggcca cgaaattgcc gtagagcggg atgctgaacg
      781 gcaggtgcag tatgacgtcg ggggcagaca ggtccgaggc ccacaggaag ccctgctgcc
      841 ggatctcgat cgcctggggc aggaactgcc acagcgcgat gatgatgggg tactgcagga
      901 gcatcgggag gcatccgccg agcggattca cccccgtctc cttgtacatc ttcatcatcg
      961 cctcctgctg cttctgcgga ttgtccgagt acttctcctt gatcgcctcc atgcgcggct
     1021 gcagctcgcg cgtcctggcc atgttggtga aggaggactt cgtcagcggg tggaccagca
     1081 gcttgatcag gatcgagaag atgatgatga ccaggccgta gttcggcagc cacttcgcca
     1141 ggaaaacgaa cgtcgggatg aagacgtact tggccagcgg gcgcgtgacg gcctcgaaga
     1201 aatcccagcc gtagtccacc atgtcgtaca ggccgaggcc gtactcgcgg atccggtagt
     1261 attccatcgg cccgacgtac atccggtacc gatcgccgtc gggccccggt acggccatgg
     1321 cgaggcttgc ggagtatccg tagcggacgg ccgggtccgc cgcctcgccg aaccgctctc
     1381 cgatcagctc cgcgccgcgc gtcgcaccgg aaggaatgac cgccgcgacg aagtacttcg
     1441 acttcaccgc cacccagtcc acctggccgc ggagcgagcg ctcgtcgtag gactggctcg
     1501 tcacctcgac cttttccacc tcgccgcccg accgggcgta caccccggaa tgctgtccct
     1561 cgttctcgtg gttctcctcc gtgaacggga tcgagccgtt ccagaccacc tcgtagccgc
     1621 tcgtcgtcac gaacgattcc gcgcgttcca tccgcacgtc catcacgacc tcgtaggtcc
     1681 cgggggcgaa cgtgtagacg acctcgaggg ccccgtcggc gaccggcgca cggaacgaaa
     1741 gcgtgcgctc gcggtcgtcc accacgatgc ggtccgcctc ggcatccgtt tcgaagtaga
     1801 aggccctcgt gtcgtagacc ctggaggagg gggacgtgaa cacgagcgag accgcgccgg
     1861 ccacggtaga gtccacgagc tgaacggtgg cgctcgagtc ggcgacctgg tattcggcca
     1921 gggaccacga acggatcgtc gctccgagcg tcgagaactc cgcctcgtac aggtccgacg
     1981 ttaccgtgat cgtccgctcc acgcccgtcg aggccccgac gacggtcgaa tcgtccggcg
     2041 acagggcagg aggggccccg gcctcgggcc cgatgtccgc cgccggatcg ggcagcgtgt
     2101 cccgcagcac gtcctcggtc ccggccaccg tggagtcggc gtcggttcct ggcggcgggg
     2161 cagggggggt gagccacgtg agccacacca ccatgatgat cgcgatcagg acggtcgcga
     2221 tgacgacgtt acgatccacg acagggagag cggtttatcg gcccgttccg cctgcgaggc
     2281 ggtgggcgac gagattcaga aggtgtggca ggtcccgtgg gatgcagtcg ggtgccaggg
     2341 acgcctgtcc ccggaacatg aagagcgccg tcagccgcag tccgcctgta cccacgacat
     2401 ccacgagggg tcccacgccg gtccggtagg cctcgcgcat gatccggcgg atcctgttgc
     2461 gcaccaccgc ggagcccgtc ccgcgggcga ccgcgaagcc ggcctggatc ggcacgccgc
     2521 gcggaagatc gtccgcggcg acatgccggt acataacgcg aacgcaaccg accgacgccc
     2581 catggacgtc cgaccggctg cgatcgaaga gagggcggat caggcgtctc cgcttgagcc
     2641 tgtgcgaccg gttgaggcgc atcatggggg ctcctggagg cctccgcgac gcgcgccgcg
     2701 ggattacttg cggctgcgcg tatcgctgac cgacagaacc ttgcgaccct tggaacgacg
     2761 gcggctcagg acgcggcggc cgttcctggt cgccatgcgg gagcggaagc cgtgcttgtt
     2821 ggcccgcttg cggcggctgg ggcgatacgt gggttgcatc tatccgggaa gctcgacgtg
     2881 aatgcaggtg ggaagcatcc ggctccatgc ggcggaccgc gcgattcgga cgcaggacgg
     2941 caaagatacg aattcgggcc gggtggtcaa atcgtgttat tgactctgcc tccgggtgct
     3001 cctactttat ctctctacgc gcgggaatag ctcagttggt agagcatcag cttcccaagc
     3061 tgagggtcgc gggttcgagt cccgtttccc gctccgccgc gtacgggttg cgcgccggcc
     3121 tccgcggccg gatccccgca acccgttttg cttttgcacc acgcgggaac ggcggattgc
     3181 cctgcggcac ggggcggtta gctcagttgg ttagagcgcc tgccttacaa gcaggaggtc
     3241 actggttcaa atccagtacc gcccaccact tctgacccag cacacccgag ccagccgcgc
     3301 tcgccgagtc caggcgcgcc ccggcgtctc gtccgccgcc acttctccga ccggacccgg
     3361 ctcttcttta cttcttcccg gtccggcatg tcgtccagca tcgttcgaat ctggaattgc
     3421 gatgcttgac c
//
LOCUS       DBNHQZ010000076         3905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1513014, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000076 DBNHQZ010000000
VERSION     DBNHQZ010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1513014"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16YU_01955"
     CDS             <1..431
                     /locus_tag="AC16YU_01955"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6064010.1"
                     /translation="PRSKESADHSLPYVVAAALVDRAVTPAQFTQDRILSDDIRSQLH
                     KVKVTADPEIEKVFPALQRVHVTILASDGRELVRQLDFPKGDPRNPLTDREIEQKFDA
                     LAAPVLSAGARRRVKDTVWGLDEGVSIGDLMALCTADLPG"
     gene            443..2383
                     /gene="lysF"
                     /locus_tag="AC16YU_01960"
     CDS             443..2383
                     /gene="lysF"
                     /locus_tag="AC16YU_01960"
                     /EC_number="4.2.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase"
                     /protein_id="HIR6064011.1"
                     /translation="MGQTLTARIARAHMTAGPSRPLVAGDVVSLRPRHVMTHDNTAAV
                     MQKFASLGAARILDPSQPVWPMDHDVQNRSEANLAKYATIEAFAREQGVDFYPPGTGI
                     SHQVMVAEGYVVPGSLVVGSDSHSNMYGALGALGTPVVRTDAAAIWATGEFWWQVPEA
                     VRVVLTGSLRPGVTGKDVIVTLCGLYNQGEVLNAVVEFAGEGAGSLSMDARMTIANMT
                     TEWGALAGIFPADAVTIEWLRGRHRVLASRGIERFTADDLAAWEADPPSSDPDAPYEA
                     EIELDLARVTPHVSGPDSVQVMRPVAEIARERVAIQKAYLVSCVNSRLEDLEAAARVI
                     RGRRVAPGVSLYVAAASREIQEQAERSGAWGALVDAGARVLPPGCGPCIGLGEGLLEA
                     GETGISATNRNFKGRMGSRDARAYLGSPAVVVESAVRGIITGPNLEEAEPPRHRYRRF
                     DRATPSETVHIEEGFPARIEGRLVFVPKDNLNTDGIYGKDYTYREDLTPADMARVVME
                     NYDPSFAGRIGRGDILVGGRNFGTGSSREQAATALKAAGIAMVIAGSFSQTYLRNAFN
                     NGFVCVACPRLLDHLLQRFRGEIARGDPTLIPGDIVGVDFTTGTVTLGGRSFRFVPLG
                     AVPQSLIVAGGIEPLVRARLER"
     gene            2425..3609
                     /locus_tag="AC16YU_01965"
     CDS             2425..3609
                     /locus_tag="AC16YU_01965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR6064012.1"
                     /translation="MPRHTVVTMPGDGIGLVVLPEAVRVLEAVGFDADYLHGDIGWEF
                     WKTEGNPLPDRTLDLLDRHRLGLFGAITSKPKKEAAAELDPALRDRGHVYFSPIVAMR
                     QRYNLDICIRPCRSFAGNPLNFVRQTIDGGHEEPLVDVVIFRQNTEGLYCGLEWTDPP
                     ESVRTAFATHPRFAPFADTPGPDLAISTRVFTRNACRRIVQRAFEYAREHGYRSVTVC
                     EKPNVLRETSGMMEEEARRAKADFPEIALWSTNIDAQMMWLTKNPEDYGVIVAGNMFG
                     DIVSDAFAGLVGGLGFACSGNIGDEIAVFEPTHGSAPKYAELDPHIVNPIAMILSAAM
                     LLDHVGEAERAGRIRSAVAEVIREGRVRTYDMLKIPGGPDAIRRGAATTEAMTDAVIA
                     RL"
     gene            3641..>3905
                     /locus_tag="AC16YU_01970"
     CDS             3641..>3905
                     /locus_tag="AC16YU_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018556.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase ACP"
                     /protein_id="HIR6064013.1"
                     /translation="MDESTNLHVTGEAGPRGEDIRSDAFVRVEPRRSGGLVIDLESRV
                     KVLFGDSIREAARSTAERFGVTDARIGIDDRGALPFVLGARLEA"
BASE COUNT          596 a         1328 c         1404 g          577 t
ORIGIN      
        1 acccgcgctc caaggagagc gcggaccaca gcctgccgta cgtcgttgcg gcggcgctcg
       61 tggaccgggc cgtgacgccc gcgcagttca cgcaggatcg cattctctcg gacgacatcc
      121 gctcgcagct ccacaaggtg aaggtcacgg cggatcccga gatcgagaag gtgttcccgg
      181 cgctgcagcg cgtccacgtc acgatcctgg cgtccgacgg acgcgagctc gtccggcagc
      241 tggactttcc gaagggcgat ccgcgcaatc cgctgacgga ccgggagatc gagcagaaat
      301 tcgacgcgct ggccgcgccc gtgctctcgg ccggcgcccg tcgccgcgtg aaggacaccg
      361 tctggggtct cgacgagggg gtatcgattg gcgacctgat ggccctctgc accgcggatc
      421 tgcccggctg acggggaagg ccatgggtca gacactcacc gcccggatcg cccgcgccca
      481 catgacggcc ggcccgtcga ggccgctcgt ggccggagac gtggtttcgc tccgtccgcg
      541 gcacgtgatg acgcacgaca acacggccgc cgtgatgcag aaattcgcct cgctcggcgc
      601 ggcccggatc ctggacccgt cgcagcccgt ctggcccatg gaccacgacg tgcagaaccg
      661 gtccgaggcg aacctggcga agtacgccac gatcgaggcg ttcgcccggg agcagggcgt
      721 ggacttctac ccgcccggga cgggaatcag ccaccaggtc atggtggcag agggctatgt
      781 cgttcccggg tccctggtcg tgggctcgga ttcgcactcg aacatgtacg gcgctctcgg
      841 agcgctgggc acacccgtcg tgcggacgga cgccgcggcg atctgggcca cgggcgagtt
      901 ctggtggcag gtaccggagg ccgtccgcgt cgtgctgacc ggctcgcttc gcccgggcgt
      961 gacgggcaag gacgtgatcg tcacgctctg cggcctctac aaccagggcg aggtgctcaa
     1021 cgccgtcgtg gagttcgcgg gcgagggagc agggtcgctc tcgatggacg cccggatgac
     1081 catcgccaac atgaccaccg agtggggcgc cctggcgggg atcttcccgg ccgacgccgt
     1141 gacgatcgag tggctgcgcg gcaggcaccg ggtgctggcc tcgcggggga tcgagcggtt
     1201 caccgcggac gacctggcgg cctgggaggc cgatccgccc tcctccgatc cggatgcgcc
     1261 ctacgaggcg gagatcgagc tggacctcgc gcgggtgacg ccgcacgtct cgggcccgga
     1321 ctccgtccag gtgatgcgcc ccgtcgccga gatcgcccgc gagcgcgtgg cgatccagaa
     1381 ggcgtacctc gtctcgtgcg tgaacagccg tctcgaggac ctcgaggcgg ccgcccgggt
     1441 gatccggggc cgccgcgtgg cgccgggggt ctccctgtac gtggcggcgg ccagccggga
     1501 gatccaggag caggccgagc gctccggcgc gtggggcgca ctcgtggatg ccggggcccg
     1561 cgtgctgccg cccggatgcg ggccgtgcat cggcctgggc gaggggctcc tcgaggccgg
     1621 cgagacgggc atctcggcta cgaaccgcaa cttcaagggc cgcatgggat cgcgcgatgc
     1681 ccgggcgtac ctcgggagtc ccgccgtggt ggtcgaatcg gccgtccgcg gaatcatcac
     1741 gggccccaac ctcgaagagg cggagccgcc ccggcaccgg taccggcgct tcgaccgcgc
     1801 caccccgtcc gagacggtgc atatcgaaga gggatttccg gcgcgcatcg agggtcggct
     1861 cgtgttcgtg ccgaaggaca acctgaatac cgacgggatc tacgggaagg actacaccta
     1921 ccgggaggac ctgacgcccg ccgacatggc gcgggtcgtc atggagaact acgatccgtc
     1981 gttcgccggc cgcatcggcc ggggcgacat cctggtcggc ggccggaatt tcggcaccgg
     2041 gtcgagccgc gagcaggcgg cgacggccct gaaggccgcc ggcatcgcga tggtcatcgc
     2101 gggctcgttc tcgcagacct atctccggaa tgccttcaac aacggcttcg tgtgcgtggc
     2161 ctgcccgcgg ctcctggacc acctgctgca gaggttccgc ggcgagatcg cccggggcga
     2221 tccgacgctc attccgggtg acatcgtggg cgtggacttc accacgggca ccgtgacgct
     2281 cgggggccga tccttccgct tcgtcccgct cggggcggtc ccgcagtcgc tcatcgtggc
     2341 cggcggtatc gagccgctgg tccgcgcgcg cctggagcgc tgacgcctcc cattcgcatt
     2401 caccctaccc gcaaccaaac gaccatgcct cgtcataccg tggtcacgat gccgggcgac
     2461 ggcatcggcc tcgtcgtcct tccggaggcc gtgcgcgtgc tcgaagccgt gggcttcgac
     2521 gccgactact tgcatggcga catcggatgg gaattctgga agaccgaggg caacccgctg
     2581 cccgaccgga cgctggacct cctggaccgg catcggctgg gtctcttcgg agcgatcacc
     2641 tccaagccga agaaggaggc ggccgcggag ctcgatccgg ccctgcggga tcggggccac
     2701 gtgtatttca gcccgatcgt ggccatgcgg cagaggtaca acctggacat ctgcattcgc
     2761 ccctgccgct cgttcgcggg caatccgctc aatttcgtcc ggcagacgat cgacgggggg
     2821 cacgaggagc cgctcgtcga cgtggtgatc ttccggcaga acacggaagg cctgtactgc
     2881 ggcctggaat ggaccgatcc cccggagtcg gtgcggacgg ccttcgcgac gcatccccgc
     2941 ttcgcgccgt tcgccgacac gcccggcccg gacctggcca tctcgacccg cgtgttcacg
     3001 cggaacgcct gccgccgcat cgtgcagcgg gcgttcgagt acgcccgtga gcacggatac
     3061 cgctccgtca ccgtgtgcga gaagccgaac gtgctccgcg agacgtccgg aatgatggag
     3121 gaggaggccc ggcgcgcaaa ggccgatttc ccggagatcg ccctctggtc cacgaacatc
     3181 gacgcccaga tgatgtggct gaccaagaac ccggaggatt acggcgtgat cgtggccggc
     3241 aacatgttcg gcgacatcgt gtccgacgcc ttcgcggggc tggtcggggg cctgggcttc
     3301 gcgtgctcgg gcaacatcgg ggacgaaatc gcggtcttcg agccgacgca cgggtcggcg
     3361 cccaagtacg cggagctcga cccgcacatc gtgaacccga tcgccatgat cctgagcgcc
     3421 gcgatgctcc tcgaccacgt cggggaggcg gagcgcgcag gccggatccg gtcggccgtg
     3481 gcggaggtga tccgggaggg ccgggtccgc acgtacgaca tgctcaagat cccggggggc
     3541 ccggacgcca tccggcgggg cgccgcgacc acggaagcca tgacggacgc cgtcatcgcg
     3601 cgcctctgac ggcgacacgg gagccacaac cttgcaggac atggacgaat cgactaatct
     3661 tcatgtgacg ggtgaggccg ggccccgggg cgaggacatc cgctcggacg cgttcgtccg
     3721 ggtggagccg cgccggagcg gaggcctggt gatcgacctg gagtccaggg tgaaggtgct
     3781 gttcggggac tcgatccggg aggccgcacg gtcgacggcc gagcggttcg gggtcacgga
     3841 tgcacggatc gggatcgacg accggggcgc cctgccgttc gtgctgggcg cccgcctgga
     3901 ggcgg
//
LOCUS       DBNHQZ010000077         4463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_971760, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000077 DBNHQZ010000000
VERSION     DBNHQZ010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_971760"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC16YU_01975"
     CDS             complement(<1..331)
                     /locus_tag="AC16YU_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR6064014.1"
                     /translation="MSPFKRNVYERSIYKEPAPIRDSENPFEAMVERFDVAAQILKLD
                     SGFYEYLVTPSRIHITAIPVLMDDGTLRMFEGYRVIHNEILGPSKGGIRYAEDVNLAE
                     VKALAAWM"
     gene            472..1266
                     /locus_tag="AC16YU_01980"
     CDS             472..1266
                     /locus_tag="AC16YU_01980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6064015.1"
                     /translation="MAGPVDLTPLTERRRKEIATLSRRRGRDRLGQFLVEGLRSVAAA
                     ADAGAPLVDVVVAESVKDTPDVQRLVGRLRVPAWRAPDRVVERLGDVQASQGIVAVAA
                     SGSGGAPDPAAAGRLLLLDGVQDPGNVGTLLRTAAWFGIDHAVAGPGTADFHGPKVVR
                     ASMGALWDIALSESSDLAPLVARLRVAGFEVWGADMRGTRFADWKPAARAALVLGSEA
                     AGLSAAVRDACTGTVTIPATGRVRGTESLNVAAAAAILVVRLSGAG"
     gene            1343..2563
                     /locus_tag="AC16YU_01985"
     CDS             1343..2563
                     /locus_tag="AC16YU_01985"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6064016.1"
                     /translation="MEVPALQGSGLERGLLTMEKRAPVGEQHRSFRIGVPRENSNEER
                     RVSLSPAGVQTLVADGHQVYVEKSAGDLANFPDREYSEMGAEIVQDPADLYAQSELVV
                     KVGPPTEDETRFLREKQTLISALHLGGTTQAFLKRLMELGITGIGFEFIRDSDGTLPI
                     VRMMHEIMGSMAVQIAARYLESGEGGKGIMLGGISGVPPSTVVILGAGVVGEWAARTA
                     LGFGAHVIVLDNDLGSLRVMEHYLDRRITTAMATEQYIRQAVVRADVVIGAMMTAGQR
                     APMLVSEEMVRSMRPGSVIVDAVIDQGGCVETSRPTSHSEPSFVQHGVIHYCIPNMPS
                     NAARTATFALTNVLVPYLLKIGQAGSINEALWRDVGLRNGAYVYRRHLTKKSLAAMFG
                     MPHRDIELLIASGI"
     gene            2607..3311
                     /gene="pepE"
                     /locus_tag="AC16YU_01990"
     CDS             2607..3311
                     /gene="pepE"
                     /locus_tag="AC16YU_01990"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HIR6064017.1"
                     /translation="MRLLLLSNSMNAGSGYLEHAADWLAEHFAGAGTVAFVPYAAVRF
                     GYDEYEERVARALAHTGATVRSVHRDADPASAVRSSDAVAVGGGNTFHLAHEMQRLGL
                     VEAIRERVAQGAPYAGWSAGANVASPTLRTTNDMPIIEPRSFDVLGLVPFQVNPHYLD
                     AHPDGHMGETREERLLEFVRVNPDVFVIGLREGSALRMDDGSARLLGPRTARIFRGDA
                     APYEVEPGTSLDFLLR"
     gene            3352..3969
                     /locus_tag="AC16YU_01995"
     CDS             3352..3969
                     /locus_tag="AC16YU_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR6064018.1"
                     /translation="MEIPFEQVRVLGALIEKERSTPEYYPLTLNSLTAACNQKSNREP
                     VTDLSESDVQRAIDELRRARLVGQSTGAGSRAEKFRHALAEAWSLAEPELAVLAVLML
                     RGPQTVGELRSRTGRLFAFGSMEAVEEALSGLAGRPEPLVRLLERRPGQKEARWAHLL
                     SGEPAEPPAEAEAGPAGALAQEVRALRAELESLREEFHAFRDAFT"
     gene            4022..>4463
                     /locus_tag="AC16YU_02000"
     CDS             4022..>4463
                     /locus_tag="AC16YU_02000"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937433.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR6064019.1"
                     /translation="MKAPTGTDLSCRGWHQEAALRMLMNNLDPDVAERPEDLVVYGGG
                     GKAARNRESLVRIIQTLRRLENDETLLVQSGKPVGVFRTHDEAPRVLIANAHLVPRWA
                     TWEEFRRLDALGLTMYGQMTAGSWIYIGTQGILQGTYETFAECAR"
BASE COUNT          653 a         1563 c         1604 g          643 t
ORIGIN      
        1 tcatccacgc ggcgagcgcc ttgacctcgg cgaggttcac gtcctcggcg taccggatgc
       61 cgcccttgga cggcccgagg atctcgttgt ggatcacccg gtagccctcg aacatgcgca
      121 gcgtcccatc gtccatcagg accggaatgg cggtgatgtg gatgcgcgac ggggtgacga
      181 ggtactcgta gaagccggag tccagcttca ggatctgggc ggccacgtcg aaccgctcga
      241 ccatcgcctc gaacggattc tcggagtcgc ggatgggtgc gggctccttg tagatggacc
      301 gctcgtacac gttgcgcttg aatggagaca tcgatcgtgg gaagaggacg gaactgcgcc
      361 cgacgagcgc ttccgggtgt cgggggtcga gccgcgcggg acgcgtatct tgcgcggaat
      421 gatcaaagta cctcgcctcg ccgttccgtg cgacagaagg atcgttgaat cttggccggc
      481 cccgtggacc tgaccccgct caccgaacgt cgcaggaagg agatcgcgac gctgtcgcgc
      541 cggcgcgggc gtgaccggct cgggcagttc ctcgtcgaag gcttgcggtc ggtcgccgcg
      601 gccgccgacg cgggggcgcc gctcgtggac gtggtcgtcg ccgagtcggt gaaggacacg
      661 ccggacgtgc agcgtctcgt cggacgcctg cgcgtgcccg cgtggcgggc gccggaccgc
      721 gtcgtggagc gcctgggcga tgtgcaggcc agccagggga tcgtggcggt cgcggcctcg
      781 gggagcggcg gggcgccgga tccggcggcg gccgggcggc tcctcctgct cgacggcgtg
      841 caggaccccg gcaacgtggg gacgctcctg cggacggccg cgtggttcgg catcgatcac
      901 gcggtcgcgg ggcccggcac ggccgatttc cacgggccga aggtggtccg ggcgagcatg
      961 ggggcgctat gggacatcgc cctctcggaa tcgtccgacc tcgcgccgct cgtcgcccgc
     1021 ctgcgggtgg ccggcttcga ggtctggggg gccgacatgc ggggcacccg attcgccgac
     1081 tggaagcctg ccgcgcgcgc cgcgctggtc ctcggatcgg aagccgcggg gctgtcggcc
     1141 gcggtccggg acgcgtgcac gggcaccgtg acgataccgg cgaccggacg cgtccgcggg
     1201 accgaatccc tgaacgtcgc cgccgcggcc gccatcctgg tggtccggct ctcgggcgcg
     1261 ggttgaaggc cggcgccggc acccccctcg caatcccgcg ccgcctcgta tattttccgg
     1321 atcattcgtc ggaggacgcg gcatggaagt gccagccctg cagggatcgg gcctcgagcg
     1381 ggggctgctg accatggaga agcgggctcc cgtgggcgag cagcaccgct cgttccgcat
     1441 cggcgtccct cgggaaaact cgaacgagga acgccgcgtg tccctctcgc ccgccggcgt
     1501 gcagacgctg gttgcggacg ggcaccaggt gtacgtggag aagagcgccg gcgacctggc
     1561 caacttcccc gaccgcgaat actccgaaat gggggccgag atcgtccagg atccggccga
     1621 cctgtacgcc cagtcggaac tcgtcgtgaa ggtcggcccc ccgacagagg acgagacccg
     1681 cttcctgcgc gagaagcaga ccctgatctc ggcgctgcac ctgggcggca ccacgcaggc
     1741 cttcctgaag cgcctgatgg agctcggcat cacgggcatc ggcttcgagt tcatacgcga
     1801 ttcggacggt accctgccca tcgtgcgcat gatgcacgag atcatgggat cgatggccgt
     1861 ccagatcgcg gcccggtacc tggagagcgg cgagggcggc aagggcatca tgctcggcgg
     1921 catctccggc gtcccgcctt ccaccgtggt gatcctgggg gcgggcgtag tcggcgagtg
     1981 ggccgccagg acggcgctcg gcttcggcgc gcacgtcatc gtgctcgaca acgacctggg
     2041 ctccctgcgc gtcatggagc actacctgga ccggcgcatc accacggcga tggcgacgga
     2101 gcagtacatc cggcaggccg tggtccgcgc ggacgtcgtg atcggcgcca tgatgaccgc
     2161 cggccagcgg gcgcccatgc tcgtgagcga ggaaatggtc cggtcgatgc ggcccggctc
     2221 ggtgatcgtg gacgccgtga tcgaccaggg cggctgcgtg gagaccagcc gccccacgag
     2281 ccactcggag ccgagcttcg tccagcacgg cgtgatccac tactgcatcc ccaacatgcc
     2341 gtcgaacgcc gcccggacgg ccaccttcgc gctcacgaac gtgctcgtgc cgtacctgct
     2401 gaagatcggc caggccggct ctatcaacga ggcgctgtgg cgggacgtcg gcctgcgcaa
     2461 cggcgcctac gtataccgcc gccacctgac caagaagagc ctggcggcga tgttcggcat
     2521 gccgcaccgg gacatcgagc tcctgatcgc ctcgggcatc tgaccgcccg gcttccgtct
     2581 cccgaaacct gccgatcccg ttcgccatgc gcctgctcct gctcagcaac tcgatgaacg
     2641 ccggctccgg gtacctggag cacgccgccg actggctggc ggagcacttc gcgggtgccg
     2701 ggacggtggc gttcgtgccg tacgcggcgg tccggttcgg gtacgacgaa tacgaggagc
     2761 gcgtggcccg ggcgctcgcc cacacgggag cgaccgtgcg gagcgtgcac cgggacgcgg
     2821 atccggcctc ggccgtacgg tcgtccgacg ccgtcgcggt cggcggcggc aacacgttcc
     2881 acctggccca cgaaatgcag cggctggggc tcgtcgaggc catccgggag cgcgtcgcgc
     2941 agggtgcgcc gtacgcgggc tggagcgcgg gggcgaacgt cgcctccccg acgctccgca
     3001 ccacgaacga catgcccatc atcgagccgc gatcgtttga cgtgctgggc ctggtcccct
     3061 tccaggtcaa cccgcactac ctggacgccc atccggacgg tcacatgggc gagacgcgcg
     3121 aggagcgcct gctcgagttc gtgcgcgtga acccggacgt cttcgtcata ggcctccggg
     3181 aggggtcggc cctgcgcatg gacgacggct ccgcccgcct gctcggcccc cgcaccgcgc
     3241 gtatcttccg cggcgatgcg gcgccctacg aggtggaacc gggcacttcg ctcgatttcc
     3301 ttcttcgctg acggcgccgc ggcatcgcgc ccggaaagcc accgtccgaa catggagatt
     3361 cccttcgagc aggtccgcgt gctcggcgcc ctcatcgaga aggagcgctc gacgcccgag
     3421 tactaccccc tcaccctgaa ttccctcacg gccgcctgca accagaagtc gaaccgcgag
     3481 ccggtcaccg acctctcgga gtcggacgtg cagcgggcca tcgacgagct gcgccgcgcg
     3541 cgcctcgtgg gccagtcgac gggcgcggga agccgggccg agaaattccg ccacgccctc
     3601 gccgaggcgt ggtcgctcgc ggagccggag ctcgcggtgc tggccgtcct gatgcttcgg
     3661 gggccgcaga ccgtgggcga gctccgaagc cgcacgggcc gactcttcgc gttcggatcg
     3721 atggaggccg tcgaggaggc gctctccggg ctcgccgggc ggccggagcc gctggtccgg
     3781 cttctcgagc gccggcccgg gcagaaggag gcccgctggg cgcacctgct ctcgggcgag
     3841 cccgcggagc cgcccgccga ggcggaggcc ggcccggccg gcgcgctcgc gcaggaagtc
     3901 cgggcgctgc gggccgagct cgaatccctt cgcgaggagt tccacgcgtt ccgcgacgca
     3961 ttcacctgag cggggacgga cccgcgcgca ccgaaccatg gatcccgccg tctacacgcc
     4021 gatgaaggcg ccgaccggca ccgacctgag ctgccgcgga tggcaccagg aggccgcgct
     4081 gcgcatgctc atgaacaacc tggatcccga cgtggccgag cgcccggagg acctggtcgt
     4141 gtacggggga ggtgggaagg ccgcgcgcaa ccgggagtcg ctcgtgcgga tcatccagac
     4201 gctccgccgc ctcgagaacg acgagacgct cctcgtgcag agcggcaagc ccgtcggcgt
     4261 gttccgcacg cacgacgagg cgccgcgcgt gctcatcgcc aacgcccacc tcgtgccccg
     4321 ctgggccacg tgggaggagt tccggcggct cgacgccctc gggctcacga tgtacggcca
     4381 gatgacggcc ggctcgtgga tctacatcgg cacgcagggc atccttcagg ggacgtacga
     4441 aacgttcgcc gagtgcgccc ggc
//
LOCUS       DBNHQZ010000078         2735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_428482, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000078 DBNHQZ010000000
VERSION     DBNHQZ010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_428482"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2565
                     /locus_tag="AC16YU_02005"
     CDS             <1..2565
                     /locus_tag="AC16YU_02005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6064020.1"
                     /translation="GRWLVALVKPPYDSTRAAKIDGRKDDDLPKDSLAILDLGTGRIS
                     KLGPVKSFTLPDDAGDALAVLFDKVKADSTEADSSEAAPDSAKAAPAKADSTVHDKKD
                     GTRLLLRRLSSGAEWSFDSAVEYGLAKNGAWLVYAAESKDGEADGAFAVNAETGEATA
                     MKTGEGHYRQLAIDDAGTQAAFVADADSFASGQPGFILFRAALGRAAEAVAFEGGEGL
                     PDGWWVSEHRKPGFSDSGRRLYFGTAPRPEKEEEDKRPDEEKVKLDVWSWTDPLLQPM
                     QLVQREAELKRSYAAVVQADGRVVQLATEDVPEVDLLETGDADVVIGESSLPYRKEIS
                     WDSPGYTDYYVIDAVTGERRRVLEGIRSNADASPDGRHLAWWDGSDRAWKITDTESGE
                     TVNVTAGADVRFDNEEHDWPYLPDEHGSAGWTKDGGAFLVYDDFDVWIATADGSLLSN
                     MTDGAGRRDRVRYRYVELDPEAKGIDLERPLFLSAFDETSKASGFARLERGADGPTRL
                     VMGDVRYGRPLKAKAADRLLFTRESFVDFPDLWTSGLELSGLTRLSNANPQQAEYRWG
                     TTRLVHWTSIDGERLDGILVRPDGFDPTRRYPMMVYFYERNSDGLHRHTPPRPGGSSI
                     GFAFYASRGYLVFIPDIPYKVGYPGESAMNAVMPGVTHLVDQGFVDPERIGVQGHSWG
                     GYQITYMVTQTNLFAAAEAGAPVTNMTSAYGGIRWGSGMSRMFQYEKTQSRIGGTLWD
                     AQDRYIHNSPLFQAPKVQTPIMYMHNDQDGAVPWYQGIEFFVALRRLGKPVWMLNYNG
                     ADHGLQNYWQRVDLQVRMQQFFDHYLLGAPAPVWMTDGIPAVRKGRTMGLELAGER"
BASE COUNT          446 a          924 c          975 g          390 t
ORIGIN      
        1 ggccgctggc tcgtcgccct ggtgaagccg ccctacgact cgacccgcgc cgcgaagatc
       61 gacggcagga aggacgacga cctgccgaag gattccctcg ccatcctgga tctcgggacg
      121 ggacggatct cgaagctggg cccggtcaag tcgttcaccc ttccggacga cgcgggcgac
      181 gccctcgcag ttctcttcga caaggtgaag gcggattcca ccgaggccga ttcctccgaa
      241 gccgctcccg attccgcgaa ggccgcgccg gcgaaggccg attccacggt gcacgacaag
      301 aaggacggca cgcggctcct gctccgccgg ctgtcgagcg gcgcggagtg gtcgttcgat
      361 tccgccgtcg agtacggcct cgcgaagaac ggcgcgtggc tcgtgtacgc ggccgagtcg
      421 aaggacggcg aggcggacgg ggccttcgcc gtgaacgcgg agaccggcga ggccaccgcc
      481 atgaagacgg gcgagggtca ttaccgacag ctcgccatcg acgacgccgg cacgcaggcg
      541 gcgttcgtgg cggacgcgga ctcgttcgcg tccgggcagc ccgggttcat cctgttccgg
      601 gcggcgctcg gtcgggcggc cgaggccgtc gccttcgagg gcggagaggg ccttccggac
      661 ggctggtggg tgtccgagca tcggaagccc ggcttctcgg actcgggacg gcgcctgtac
      721 ttcggcaccg cgcctcgtcc cgagaaggaa gaggaagaca agcggccgga cgaggagaag
      781 gtgaagctgg acgtctggag ctggaccgat cccctgcttc agcccatgca gctcgtgcag
      841 cgcgaggccg agctcaagcg ctcgtacgcg gcggtcgtgc aggcggacgg gcgcgtggtg
      901 caactcgcga cggaggacgt gccggaagtg gacctgctgg agacgggcga cgccgatgtc
      961 gttatcgggg agtcgagcct cccgtaccgc aaggagatct cgtgggactc gcccggctac
     1021 acggactact acgtgatcga cgccgtcacc ggagaacgcc ggcgggtgct cgagggcatc
     1081 cgctcgaacg ccgacgcttc gcccgacggc aggcacctcg cgtggtggga cggaagcgac
     1141 cgcgcgtgga agatcacgga caccgaatcg ggcgagaccg tgaacgtcac cgcgggcgcc
     1201 gacgtgcgct tcgacaacga ggagcacgac tggccctacc tgccggacga acacggctcg
     1261 gccgggtgga cgaaggacgg cggcgccttc ctggtctacg acgacttcga cgtgtggatc
     1321 gcgacggccg acggctcgct cctctccaac atgaccgacg gcgcgggccg gcgcgaccgc
     1381 gtccgctacc gctacgtgga gctggacccg gaggcgaagg gcatcgacct ggagcggccg
     1441 ctcttcctct cggccttcga cgagacgtcg aaggcgtcgg gcttcgcgcg gctcgagcgg
     1501 ggcgccgacg gcccgacgcg gctcgtgatg ggcgacgtcc ggtacggccg cccgctgaag
     1561 gcgaaggcgg cggaccggct gctcttcacg cgggagtcgt tcgtggactt tccggacctg
     1621 tggacgtccg ggctcgaact gtcggggctc acgcgccttt cgaacgccaa cccgcagcag
     1681 gccgagtacc gctggggtac gacgcgcctc gtgcactgga catcgatcga cggcgagcgc
     1741 ctcgacggca tcctggtccg ccccgacggc ttcgacccga cccgccgcta ccccatgatg
     1801 gtgtacttct acgagcggaa ctccgacggc ctgcaccgcc acacgccgcc ccggccgggc
     1861 gggtcgtcga tcgggtttgc gttctatgcg agccgcgggt acctcgtgtt catccccgac
     1921 atcccgtaca aggtgggata ccccggcgag agcgccatga atgccgtgat gccgggggtg
     1981 acgcacctcg tcgaccaggg cttcgtggac ccggagcgga tcggcgtaca ggggcactcg
     2041 tggggcggat accagatcac gtacatggtc acgcagacca acctgttcgc ggccgccgag
     2101 gcgggcgcgc ccgtcacgaa catgacgagc gcgtacggcg ggatccgctg gggctcgggc
     2161 atgagccgca tgttccagta cgagaagacc cagagccgga tcggcggcac gctgtgggac
     2221 gcccaggacc ggtacatcca caactcgccg ctattccagg ctccgaaggt ccagacgccc
     2281 atcatgtaca tgcacaacga ccaggacggc gccgtgccgt ggtaccaggg catcgagttc
     2341 ttcgtggcgc ttcgccggct cggcaagccc gtgtggatgc tgaactacaa cggcgcggac
     2401 cacgggctcc agaactactg gcagcgcgtg gacctgcagg tgcgcatgca gcagttcttc
     2461 gaccactacc tgctcggcgc gcccgctcca gtctggatga ccgatggcat cccggcagtg
     2521 aggaagggcc ggacgatggg gctcgagctc gccggggaac ggtagagacc ggaatcgtcg
     2581 cccccccgaa acgacgggcg ccgcgccgcc tccagggcag cgcggcgctc gggtctccag
     2641 ctttcgccgt ggaccgggga tcagccctcg ccctgctcgg cctcgagctg ggcgcggtac
     2701 tcctcggtgg ccgtgatcgc gatttccaca cggcg
//
LOCUS       DBNHQZ010000079         5704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_428660, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000079 DBNHQZ010000000
VERSION     DBNHQZ010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_428660"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..2028
                     /gene="tkt"
                     /locus_tag="AC16YU_02010"
     CDS             10..2028
                     /gene="tkt"
                     /locus_tag="AC16YU_02010"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6064021.1"
                     /translation="MSQAASVPETETDLLAVNALRFLAVDAVQKANSGHPGMPMGAAA
                     MAFALWSRHLKHDPSNPDWFDRDRFVLSAGHGSMLLYGLLHVFGYDLPMDELRRFRQW
                     GSRTPGHPERGDTPGVETTTGPLGQGLANGVGMALAERLLAARFNRPGHTVVDHHTYG
                     IVSDGDLMEGISHEAASLAGHLGLGKLIYLYDDNGISIDGPTSLSYSDDAAARFAAYG
                     WHVVRVEDGNDLDAVDEALDEARAEHARPSLVLVRTHIGFGSPAKQDTAGVHGSPLGP
                     DEVLATKRNLGWPESPAFHVPDAVYRHAAESTRRGRAAREKWTAGLEAYAAEFPDEAA
                     EFRAWTGGALPADWEQALPRYAAGDRMATRKASGNAIAALAGRIGNLVGGSADLTPSN
                     NTAVPGRDAQSHQEPGGRYFHFGVRELGMAAISNGLVLHGGLRPYCGTFLVFSDYMRP
                     AVRLSAIMRQPVIYVYTHDSIGLGEDGPTHQPVEHLAALRAIPGLVVLRPADASETSE
                     AWRIAIARTDGPTALALTRQDTVVSPETADRASGGVRRGGYVLREGGDRPDAVLIATG
                     SEVGLALAAADLLESEGTRVRVVSMPSWELFEAQPQAWRDSVLPPGVTRRVAVEAGVS
                     LGWARYVGPAGRTVSMDRFGASAPDEVLFEQFGFTPERVADAVRSTAG"
     gene            2097..3836
                     /locus_tag="AC16YU_02015"
     CDS             2097..3836
                     /locus_tag="AC16YU_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6064022.1"
                     /translation="MPEPVSRRSFIASASLAGAAAALGVPSGSARARSVAEAVAEVAG
                     PSGPITVETIAAAEVVAGISFTPEQRELMLEDLQERLEMFDRLRAVPLPNAVTPSLLF
                     DPRLGGATPAPPSEAGISWEPSPGGRPTSAEDLAFATVAELSGLLRQRAVTSVELAEL
                     YLDRLQRLDDRLHAVVTLTSERALDQARAADAELDAGRWLGPLHGVPWGAKDLLAVRG
                     HPTTWGAMPYRDQVIDLDAAVVERLDAAGAVLVAKLTLGALAWGDVWFGGRTNNPWNP
                     EQGSSGSSAGPGAAVSAGLVGFAMGSETLGSIVSPATRNGVTGFRPTFGRVSRHGAMT
                     LSWSMDKLGPMARCAEDCALVFAALHGRDPRDPSTTDARFRWPFPLRPDRLRVGYVAE
                     AFEGEYDGSAADAAVLGVLRDLGFEPEPVALPDLPADAVTMMLEAEAAAAFDELTRSG
                     GVDSMVRQERNAWPNVFRHARLIPAVEYINASRVRTLLMREMGELFAAWDVVVCPSFR
                     GGVLGITNLTGHPSVTVPNRFDPVEDDASGTLRAPGSITFLGGLYRDDQVLAAAHAYQ
                     QATDFHRRRPPVR"
     gene            3833..4753
                     /locus_tag="AC16YU_02020"
     CDS             3833..4753
                     /locus_tag="AC16YU_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TroA family protein"
                     /protein_id="HIR6064023.1"
                     /translation="MTGPRTLSLVPDASDWMRLLGLEGFLAGGTEVCRGRMDLPACTR
                     PAWRPAPVRALVPSEPAERDPGRDLDVEAVRAVDPELVIVDAWNETWTEGPGDWLGEL
                     LGHPVETWTWNVRTLRDALEQGLRLGRRFGAMGRTMQVLGDREAELLSWRSRTGLHRR
                     APDGQLDPVTVISSLDPPAAAAGWTSEVVDRAAARLSTARSGEPAETRSWESLPLGPD
                     VFAIVSLPGRSADEACRVLGEMPGATTALGRAGRVVCYDGRGTFDGAGPGLHQAILDI
                     MALVHGFGDRNPGARSVAWPVPETGTAGSG"
     gene            4786..5145
                     /locus_tag="AC16YU_02025"
     CDS             4786..5145
                     /locus_tag="AC16YU_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR6064024.1"
                     /translation="MDINFYKLLHILGILLVFQGIGASVALARSESGAPWRRASAIGH
                     GIGMILLLVAGFGMLAKLGIMASMPGWVWAKLVIWLILGALPTVLRKSPGAVRWVWGG
                     AIGLGVLAASMALFKPF"
     gene            5148..5672
                     /locus_tag="AC16YU_02030"
     CDS             5148..5672
                     /locus_tag="AC16YU_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfiT family bacillithiol transferase"
                     /protein_id="HIR6064025.1"
                     /translation="MDDLAYPIGRFRASQDHSPVARRDRIEAIAELPGRLSRAVEGLD
                     DVQLDTAYRPGGWTIRQLVHHVADSHVNAVVRLRLALTEDGPTIKPYNQEAWASLPDA
                     TGGPVGPSLEILQGLHERWVALLRTLPPGAFSRTAVHPEVGPITVDWILDQYSWHGRH
                     HVAQILAARARLGW"
BASE COUNT          753 a         2043 c         2078 g          830 t
ORIGIN      
        1 cggagcccga tgtcccaggc cgcttccgtc cccgaaaccg aaacggatct cctagccgtc
       61 aacgccctgc gattcctcgc cgtagacgcg gtgcagaagg cgaacagcgg ccatccgggc
      121 atgcccatgg gtgcggcggc catggcgttc gcgctctgga gccggcacct gaagcacgat
      181 ccctcgaatc cggactggtt cgaccgcgac cgcttcgtgc tctcggccgg gcacggctcg
      241 atgctcctgt acgggctcct gcacgtgttc ggctacgacc tgccgatgga cgagctccgc
      301 cggttccggc agtggggaag ccgcacgccc ggccaccccg agcgcgggga cacgcccggc
      361 gtcgagacca cgacgggacc gctcggccag ggcctggcga acggcgtcgg aatggccctc
      421 gccgaacgcc tgctcgcggc gcggttcaac cgccccggac acacggtcgt cgaccaccac
      481 acgtacggga tcgtgtcgga cggcgacctg atggagggca tcagccacga ggcggcctcg
      541 ctggcaggcc acctggggct gggcaagctc atctacctgt acgacgacaa cggcatctcg
      601 atagacggtc cgacgtccct ctcgtacagt gacgacgcgg ccgcgcggtt cgcggcctat
      661 ggctggcacg tggtcagggt cgaggacggc aacgatctcg acgcggtgga cgaggcgctc
      721 gacgaggcgc gggcggagca cgcgaggccg tccctggtcc tggtccggac gcacatcggg
      781 ttcggctccc cggccaagca ggacacggca ggggtccacg ggtctcccct cggaccggac
      841 gaggtcctgg cgaccaagcg caacctgggc tggccggaat cgcccgcgtt ccacgttccg
      901 gacgcggtgt atcggcacgc cgcggagagc acccgaaggg gccgagcggc ccgggagaag
      961 tggaccgccg ggctcgaggc gtacgccgcg gaattccccg acgaggccgc ggaattccgg
     1021 gcgtggacgg gaggcgcgct gccggccgac tgggagcagg cgctgccccg ctacgcagcc
     1081 ggcgaccgga tggcgacgcg gaaggcgagc gggaatgcga ttgccgcgct ggctgggcgc
     1141 ataggcaacc tggtcggcgg atccgccgac ctgacgccgt ccaacaacac ggccgttccg
     1201 ggccgggacg cccagtcgca ccaggagccg ggcggccgct acttccactt cggggtgcgg
     1261 gagctgggca tggccgcgat ctcgaacggc ctcgtgctgc acggagggct tcgaccgtac
     1321 tgcggcacgt tcctggtctt cagcgactac atgcggccgg ccgtccgcct gagcgcgatc
     1381 atgcggcagc ctgtgatcta cgtctacacg cacgactcga tcggtctcgg cgaggacggt
     1441 cccacgcacc agccggtcga gcatctcgcg gccctcaggg ccattccggg cctcgtggtg
     1501 cttcggccgg ccgatgcctc ggagacgtcc gaggcgtggc ggatcgcgat cgcccgcacg
     1561 gacgggccca cggcgctggc gctcacgcgg caggacaccg tcgtgagtcc ggagaccgcg
     1621 gaccgcgcgt ccggtggcgt ccggcgggga ggctacgtgc tccgcgaggg gggagaccgg
     1681 cccgatgccg tgctcatagc cacgggcagc gaggtcggcc tggcgctcgc ggccgccgac
     1741 ctcctcgaat ccgagggtac ccgggtccgc gtagtcagca tgccgtcctg ggaactcttc
     1801 gaggcgcagc cgcaggcctg gcgggactcg gtcctgccac ccggcgtgac gcgccgggtc
     1861 gcggtggagg ccggcgtttc cctgggctgg gcgcgctacg tgggcccggc cggccggacg
     1921 gtgtcgatgg accgcttcgg cgcgtccgct cccgacgagg tactcttcga gcagttcggc
     1981 ttcacgcccg aacgtgtcgc ggacgccgta cggtcgaccg cgggttgaag cgcgcgccgg
     2041 ccggtttcgg cccgcgtccg ccgggtgtac tttccggaaa accagcccgg agtcccatgc
     2101 ccgagcccgt ctcccgccgc tcgttcatcg cctcggcctc gctggccggt gccgccgcgg
     2161 cgctcggagt cccgagcgga tccgccaggg cccggtctgt cgccgaggcc gtcgccgagg
     2221 tggccgggcc gtccggtccg atcaccgtcg aaaccatcgc cgccgcggag gtcgtggccg
     2281 gcatctcgtt cacgcccgag cagcgcgagc tgatgctcga ggacctgcag gagaggctcg
     2341 agatgttcga ccggctccgc gcggtgccgc tcccgaacgc cgtcactccg tcgctgctct
     2401 tcgatccccg gctggggggc gccacgcccg cgccgccctc ggaggccggg atctcctggg
     2461 agccctcgcc cggcggtcga ccgacgagcg ccgaggacct cgccttcgcg accgtggcgg
     2521 agctctccgg cctcctgcga cagcgcgcgg tgacgagcgt cgagctggcg gagctgtacc
     2581 tggaccgact tcagcggctc gacgaccgtc tccacgccgt cgtcacgctc acgagcgagc
     2641 gggccctgga ccaggcccgg gcggcggacg ccgagctcga tgcgggccgc tggctcgggc
     2701 ccctgcacgg cgtgccctgg ggcgcgaagg acctcctcgc agtgcggggc catccgacca
     2761 cctggggggc gatgccgtac cgtgaccagg tgatcgacct ggatgcggcc gtggtcgagc
     2821 ggctggatgc cgccggtgcc gttctcgtgg ccaagctcac gctgggggcc ctggcctggg
     2881 gcgacgtctg gttcggggga cggacgaaca acccgtggaa ccccgaacag ggatcgagcg
     2941 gttcgtcggc cggcccgggg gccgccgtgt cggcgggcct ggtgggcttc gcgatgggct
     3001 cggagacgct gggatccatc gtgtcgccgg ccacgcggaa cggcgtgacg ggcttcaggc
     3061 ccaccttcgg ccgcgtatcg cgccatgggg cgatgaccct gtcgtggtcc atggacaagc
     3121 tcggcccgat ggcgcgctgc gcggaagact gcgctctcgt attcgcggcg ctgcacggcc
     3181 gcgacccccg cgatccgtcc acgacggatg cccggttccg gtggcccttc ccgttgcggc
     3241 ccgaccggct gcgcgtggga tacgtcgccg aggcgttcga gggggaatac gacggatcgg
     3301 cggccgacgc ggccgtgctc ggcgtgcttc gcgacctcgg cttcgagccg gagcccgtgg
     3361 cgcttccaga tcttccggcc gacgccgtga cgatgatgct cgaggccgag gcggcggcgg
     3421 cgttcgacga gctcacccgg tcgggcgggg tcgattccat ggtccgccag gagcggaatg
     3481 cctggcccaa cgtgttccgc cacgcccgcc tcattccggc cgtggaatac atcaacgcga
     3541 gccgggtccg caccctgctc atgcgcgaga tgggcgagct gttcgccgcc tgggacgtcg
     3601 tcgtgtgccc cagtttccgc ggaggcgtgc tcggcatcac caacctcacg ggtcatccgt
     3661 ccgtgactgt cccgaaccgg ttcgatccgg tcgaggacga cgcatccggc acgctgcgcg
     3721 cgccgggcag catcactttc ctgggcgggc tctaccgcga cgaccaggtg ctcgccgccg
     3781 cccacgcgta ccagcaggcg accgatttcc atcggcgccg gccgcccgtc cgatgaccgg
     3841 cccgcgcacc ctttccctgg tgccggacgc gtcggactgg atgcggctgc tcgggctgga
     3901 gggcttcctg gccggtggaa cggaagtctg ccgcggacgc atggatcttc cggcttgcac
     3961 ccgcccggcc tggcggcctg cgcccgtccg ggcgctcgtg ccgtcggagc ctgcggagcg
     4021 ggatccgggg cgggacctcg atgtcgaggc cgtgcgcgcg gtcgatcccg agctcgtcat
     4081 cgtggacgcc tggaacgaaa cctggacgga gggtccgggc gattggctcg gggagctgct
     4141 gggccacccg gtcgagacct ggacgtggaa cgtccgcacg ctgcgtgatg ccctggagca
     4201 gggcctccgg ctgggtcgcc gcttcggggc gatgggccgg acgatgcagg tcctgggcga
     4261 ccgggaggcg gagctcctgt cctggcgctc gcgcacgggc ctgcaccggc gggcgcccga
     4321 cgggcagctg gatccggtca cggtcatctc ctcgctcgat ccgcccgccg ccgccgccgg
     4381 ctggacgtcc gaggtcgtgg accgggcggc ggcacggctc tcgaccgccc gctccggaga
     4441 gccggccgag acccggtcct gggagtcgct gccgctcggg ccggacgtgt tcgcgatcgt
     4501 gagcctcccc ggccggagtg ccgacgaggc atgccgcgtg ctgggcgaga tgcccggggc
     4561 gaccactgcg ctcggccgcg cggggcgcgt cgtctgctac gacggccggg gcaccttcga
     4621 cggcgcaggg cccggcctgc accaggccat actcgacatc atggccctgg tccatggatt
     4681 cggcgaccgg aatccgggcg cccgctccgt cgcatggccc gtcccggaga ccggaactgc
     4741 cggttcgggt tgaagtacgc ccacccaccc gaaccgagac gacccatgga catcaatttc
     4801 tacaagctgc ttcacatcct gggcatcctg ctcgtcttcc agggcatcgg cgcgtcggtc
     4861 gccctggcac ggtccgagtc cggggcgccg tggcgcaggg catcggccat cgggcacgga
     4921 atcggcatga ttctgctgct cgtcgccggg ttcggcatgc tggccaagct gggcatcatg
     4981 gcctcgatgc ccggatgggt gtgggccaag ctcgtgatct ggctgatcct gggcgcgctg
     5041 cccaccgtgc tccggaagtc gccgggcgcg gtccgctggg tctggggcgg agcgatcgga
     5101 ctcggcgtgc tcgcggcctc catggcgctc ttcaagccgt tctgaggatg gacgacctgg
     5161 cctatcccat cgggcggttc cgggcgtcgc aagaccactc gccggttgcc cgacgggacc
     5221 ggatcgaggc catcgcggag cttcccggcc ggctctcgcg cgcggtcgag ggcctcgacg
     5281 acgtccagct ggacacggcg taccggcccg gcggatggac catccggcaa ctcgtgcacc
     5341 acgtcgcgga cagccacgtg aacgccgtcg tgcgcctccg cctggcactc accgaggacg
     5401 ggccgacgat caagccctac aaccaggagg cctgggcgtc gttgcccgat gcgaccggcg
     5461 gtcccgtcgg gccgtcgctc gagatcctgc aggggctgca cgagcggtgg gtcgccctgc
     5521 tgcggaccct gcccccggga gccttctcgc gcaccgctgt ccacccggaa gtcggaccga
     5581 tcacggtgga ctggatcctg gaccagtaca gctggcacgg ccggcaccac gtcgcccaga
     5641 tcctggcggc aagggcgcgc ctcggctggt agcgcgaaga gtcagaattc gtaccgcacc
     5701 tgca
//
LOCUS       DBNHQZ010000080         5248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_158160, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000080 DBNHQZ010000000
VERSION     DBNHQZ010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_158160"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..915)
                     /locus_tag="AC16YU_02035"
     CDS             complement(232..915)
                     /locus_tag="AC16YU_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064026.1"
                     /translation="MAPRRKLALNAGAISLLMFAAFVLMGTRGNPGLADASSDPVARG
                     RFLVLTHDCGGCHGGLANPAGPGWLTGYDAETNPDVAFKIGPFTTFPRNLTPDNTTGL
                     GRFSERQIFNALRYGLRPGETPDVEITSSTPGEGNFPEHPKYLAPPMPWPAWRHMPDE
                     DLRAIAAYLKRGLKPVEHFVPDSEGPPDFWAEEYAKDYWGTYPAPAFPTANEVNPAPS
                     AGGSGNGRP"
     gene            complement(927..2363)
                     /locus_tag="AC16YU_02040"
     CDS             complement(927..2363)
                     /locus_tag="AC16YU_02040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR6064027.1"
                     /translation="MSGRRRARLTGGSVLFAFLVGLGLAGRAVAQAPAPERSAFSEPA
                     PADIRASDDGTLEVWLTAAPTGTSLLPGTETDVFAYNGQIPGPTIEVREGDRLIVHFR
                     NDLPEPTTVHWHGLHIPVSSDGSPLNLVQPGETFDYDLMIDPGTAGTYWYHPHPDHRT
                     GFQVGKGLFGALIVRADDDPLAGLAPEKLIVLTDNRFGDDGSVHFPDPSSLAGRIDFE
                     NGREGDVLFVNDRVMPTLSIGRGETQRWRIVNASGSRVYRLSLGGRPFLHVGDDGGLF
                     ERPREVTELLMANSERVEILVRGEGPPGTRTMLQSLPYDRYIRQTRPADWETPLDLLA
                     VEVSDAPPSEPVRIPEVLRPVAPLDTSDIAATRVQVLTQGLINGRHMDMNRVDVSARL
                     GTTEIWQVENLVGMDHPFHLHGFRFQVLSRNGVPTEYLSWKDTVNVPKHETVRFVVRF
                     DDHPGKWMFHCHILDHEDAGMMGILEVN"
     gene            complement(2534..2932)
                     /locus_tag="AC16YU_02045"
     CDS             complement(2534..2932)
                     /locus_tag="AC16YU_02045"
                     /inference="COORDINATES: protein motif:HMM:NF040487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-virulence regulator CigR family protein"
                     /protein_id="HIR6064028.1"
                     /translation="MTASRLRLLLVAVAMAGLALGPGDARAQNRRSHDRGSGHDLVSL
                     TVGLSSQIRDFYAARPATGAKPLPPGMRNRLARGKPLPPGIAKQVAPAALRSHLALPR
                     GYDLVEVGLDVLLVEVATGVIHDVLMDVVR"
     gene            complement(3026..>5248)
                     /locus_tag="AC16YU_02050"
     CDS             complement(3026..>5248)
                     /locus_tag="AC16YU_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064029.1"
                     /translation="TYDVVVVNDAGGEAVLPGGFRVEPATRLAIDTQVAAPDAIRGGS
                     SATYGLTFSNNGNTDIPYLSIEAATSFALDIDVVASDGLLTLTDILPDAFNPAGHDFV
                     YSREKMASIPMLYRNLPPGGSATASITFEDPEDPLLLSLYANPLTAPQYVEQLRSHFA
                     TLREQIMLHRTDVDPFFVGLAGDREEFDRRYFRVYVALGILEPEEGIGLPDGLLIAGK
                     RSSGPAAPTLDGQNNEYNFCFVLFEDGTDQEVWPCPPDPPPPPPNPSPPAPTPTPPPT
                     PDPGPELPGPCASYDRCLEIKARQCGYAGVLHCAALGGLAGILTGGVGGIVLGGLCSA
                     LYVNACDTSNPNSLPRSICTPVKQSCDPNDIIGPAGYGAAKWIRRDATLTYTVRFEND
                     PLKATAPAQVVGITHPLAETADVRRFRLGSFGFGPFSFHPPENSAHYSTRLDVTDSLG
                     VYVDVTAGLDVIGSRAFWVFRSVDPETGDQPSNDPFAGFLPVNDDAGAGEGFAEYSIA
                     PAENAATGDRLNALARIVFDINPPLDTPEVFNTIDASQPVSSVAVQPGRQDTTAFHVS
                     WSGTDEGSGVRDFELYVSTDEGPFARYQEATSGTSLLFIAEKERTYRFFTIATDHVGN
                     REPMKTEGEAVTVVGVEEEEPGLPASFALHQNYPNPFNPRTTIPFDIAQHADVQIVVY
                     NVLGQRVLVARDESMSPGHYQQTIDMSQFASGVYFYEIRVLGTGQARFRDVRKFVLVK
                     "
BASE COUNT          877 a         1660 c         1853 g          858 t
ORIGIN      
        1 gaccgggccg tcgagctcgt ggccgaggcc gtccccggca ggtcaccgga cgacatcctg
       61 gcccggctca tggagcagac ggacgcgagg agggccggac agcctccgaa cgacgacgtc
      121 accttcgtgg tgctgaaggc atccgtcgtc gagtagggtt ccatccggcc cgtcgcgatc
      181 gctaccgatc gccggagccc tgcaccgcgc ccggcagccg tcccgcggcg gtcagggccg
      241 gccgttcccg ctgccgcccg cggacggggc aggattgacc tcgttggccg tcgggaacgc
      301 cggggcaggg taggtgcccc agtagtcctt cgcgtactct tccgcccaga agtccggcgg
      361 tccctcggag tcgggcacga agtgctcgac gggcttgagc ccgcgcttca ggtacgcagc
      421 gatggccctc aggtcctcgt cgggcatgtg ccgccacgcc ggccacggca tcgggggggc
      481 caggtacttc ggatgctcgg ggaaattccc ctcaccgggc gtcgacgacg tgatctccac
      541 gtcgggcgtc tcaccgggcc ggaggccgta gcggagcgcg ttgaagatct gccgctccga
      601 gaaacgtccc aggccggtcg tgttgtccgg ggtcaggttg cggggaaacg tcgtgaaggg
      661 gcctatcttg aatgcgacat cagggttcgt ttcggcatcg tagccggtga gccagcccgg
      721 acccgcggga ttcgcgagac cgccgtggca gccgccgcaa tcgtgcgtca ggaccaggaa
      781 gcggccccgg gcgaccgggt cgctcgaggc gtcagccagc cccgggtttc cccttgtgcc
      841 catcagcacg aaggcggcga acatgagcag ggagattgcg cccgcgttca gcgcgagctt
      901 ccgtcgtggg gccatggggt tctccgtcaa ttgacttcaa gtatgcccat catcccggcg
      961 tcctcgtggt ccaggatgtg gcagtgaaac atccattttc cggggtggtc gtcgaatcgg
     1021 accacgaacc ggaccgtctc gtgcttgggc acgttgaccg tgtccttcca gctcaggtac
     1081 tctgtcggga cgccgttgcg cgagagcacc tggaagcgga atccgtgcag atggaaagga
     1141 tggtccatgc cgaccaggtt ctccacctgc cagatttcgg tcgttccgag gcgcgcgctc
     1201 acgtcgacgc ggttcatgtc catgtgacgc ccgttgatta ggccctgggt gagcacctgc
     1261 acgcgcgtgg ccgcgatgtc gctcgtgtcg agcggcgcga ccgggcgcag gacttcggga
     1321 atgcggacgg gctctgaggg cggcgcgtcc gagacttcga ccgccaggag gtcgaggggc
     1381 gtctcccagt cggcggggcg cgtctgccgg atgtaacggt cgtagggaag cgactgcagc
     1441 atggtccggg tgccgggagg gccctcgccc cggaccagga tttccacgcg ctcggaattg
     1501 gccatgagga gctccgtcac ctcgcggggc ctttcgaaca gaccgccgtc gtcgccgacg
     1561 tggaggaaag gccggccgcc gagcgacagc cggtacacgc gggatccgga cgcgttcacg
     1621 atgcgccagc gctgggtctc gccgcggccg atcgacagcg tcggcatcac ccggtcgttc
     1681 acgaacagga cgtctccctc gcgcccgttc tcgaagtcga tgcgcccggc gagcgaggag
     1741 ggatccggga agtgcaccga gccgtcgtca ccgaaccggt tgtccgtgag gacgatgagc
     1801 ttttcggggg cgaggccggc caggggatcg tcgtccgcac ggacgatcag tgcgccgaag
     1861 aggcctttcc ccacctggaa accggtgcgg tggtcggggt gaggatggta ccagtaggtg
     1921 ccggccgtgc ccggatcgat catcaggtcg taatcgaagg tctcgcccgg ctgcaccagg
     1981 ttcagcgggc tgccgtcgga ggagacggga atgtgaagcc cgtgccagtg gacggtggtg
     2041 ggttcgggca ggtcgttccg gaaatgcacg atgagccggt cgccctcccg cacctcgatc
     2101 gtcgggccgg ggatctgccc gttgtaggcg aagacgtccg tttcggtgcc gggcagcagc
     2161 gaagtcccgg tcggggcggc cgtcagccag acctcgagcg tgccgtcgtc cgaagcgcgg
     2221 atgtcggccg gcgccggctc ggagaatgcc gaacgctccg gggcaggggc ctgggccacg
     2281 gcccggcccg cgaggccaag gccgaccagg aaagcgaaca ggacggagcc gccggtcagg
     2341 cgtgcccgac ggcggccgga cacccgactg gctgctccgc ccggacactg gcagggcata
     2401 ggggcggtct tcccgtcagc atgtagggga tagcactaca cgccaaggta ggaatctgcg
     2461 gcacaattgc aagcccccat gcaattgtcc gggaggaccg agacagaacg gcccgaatcg
     2521 tcgcggaatc cggtcagcgc acgacatcca tgagcacgtc gtggatcacg ccggtggcca
     2581 cctcgacgag cagcacgtca aggcccacct ccacgaggtc gtagccgcgc ggaagggcca
     2641 ggtgcgaccg gagcgccgcc ggcgccacct gcttcgcgat gccgggcggc agcggcttgc
     2701 cgcgcgcaag ccggttgcgc atgccgggcg gtagcggctt cgcgcccgtc gccggccggg
     2761 ccgcatagaa gtcgcggatc tgcgaggaaa ggccgaccgt aaggctgacc aggtcatggc
     2821 cgctcccccg atcgtgggat ctgcgattct gcgcccgggc gtcgccgggg ccgagggcga
     2881 gcccggccat cgcgacggcc acgagaagga gtcggagtcg tgatgcggtc atggcgatgc
     2941 cccggcgttc atgtggtaca cacgtcgggg tatcgacctc ggcggaggca aacctaaagc
     3001 ccggttgcgg tcggctcgcc cgacctcatt ttacgagaac gaattttctc acgtccctga
     3061 accgtgcctg gccggttcca aggacccgga tctcgtagaa gtacacgccg ctggcgaact
     3121 gcgacatgtc gatcgtctgc tggtagtgtc cgggggacat cgactcgtcg cgcgcgacga
     3181 gcacccgctg tcctagcacg ttgtagacga cgatctgcac gtccgcgtgc tgcgcgatgt
     3241 cgaacgggat cgtggtccgc ggattgaatg gattcgggta gttctggtgc agggcgaacg
     3301 aggcgggcag cccaggctcc tcctcctcga ctcccaccac cgtgaccgct tccccctcgg
     3361 tcttcatggg ctcgcggttg ccgacgtggt ccgtcgcgat cgtaaagaag cggtaagtac
     3421 gctccttctc cgcgatgaag agaaggctcg tccccgaggt cgcctcctgg tagcgcgcga
     3481 acggaccttc gtccgtggaa acgtacagtt cgaagtcccg aacgcccgaa ccttcgtccg
     3541 tgccggacca cgagacgtgg aatgcggtcg tgtcctgccg cccgggctgg acggcgacgg
     3601 agctgacggg ctgcgaggcg tcaatggtgt tgaacacctc gggcgtgtcg agcggcggat
     3661 tgatgtcgaa tacgatgcgg gcgagcgcgt tgagtctgtc accggtcgcc gcattctcgg
     3721 cgggtgcaat cgaatactcg gcaaacccct ctcccgctcc tgcatcgtcg ttcacgggca
     3781 ggaagccggc aaagggatcg ttggaaggct ggtctcctgt ctccggatcg accgaccgga
     3841 atacccagaa tgcgcgactg ccgatcacgt ccagtccggc cgtcacgtcc acgtataccc
     3901 cgagcgagtc cgtcacgtcc agccgcgtcg aatagtgggc agagttctcg ggtggatgaa
     3961 aagagaacgg accgaatccg aacgatccca gccgaaagcg gcgcacgtcg gccgtctcgg
     4021 cgagcggatg cgtaatcccc acgacctggg ccggagcggt ggccttcagc gggtcgtttt
     4081 cgaatcggac ggtgtatgtg agcgtagcgt cccgacggat ccacttcgct gcgccgtagc
     4141 cggcgggccc gatgatgtcg ttcggatcgc acgactgctt gacgggtgtg caaatgcttc
     4201 gcggcagcga attcggattg ctggtgtcgc aggcgttgac atacagggcg ctgcacaaac
     4261 cgccgagcac gatgcctcca accccgcccg tgaggatgcc ggcgagccct cccagggcgg
     4321 cgcagtgcag gacacccgcg tacccgcact ggcgggcctt gatttccagg cagcggtcat
     4381 acgatgcgca gggacccggc agttcggggc cggggtccgg tgtcggcggg ggagtgggag
     4441 tcggagcagg gggagaggga ttgggagggg gagggggagg atccggtggg cagggccaga
     4501 cctcctgatc ggtcccgtcc tcgaacagga cgaagcagaa gttgtattca ttgttctggc
     4561 cgtccaaggt cggcgcggcc gggccggatg accgcttgcc cgcgatcagt agtccgtccg
     4621 gaagtccgat tccctcttcg ggttcgagaa tgccaagcgc gacgtacacc cggaagtagc
     4681 gtcggtcgaa ctcctcgcga tcaccggcca gccccacgaa gaaagggtcc acatcggtcc
     4741 gatgcagcat gatctgttcg cgcagggtgg caaagtggct gcgcagttgc tcgacgtatt
     4801 gcggggcggt cagcggattg gcatagagcg acagaaggag cggatcctcc ggatcctcga
     4861 acgtgatgct cgccgtggcc gaacctcccg gtggcagatt ccggtacagc atcgggatgc
     4921 tcgccatctt ctcacgcgag tagacgaaat cgtggccggc cggattgaac gcgtccggga
     4981 ggatatcggt cagcgtcagg agtccgtcgg acgcgacgac atcgatgtcg agtgcgaacg
     5041 aggtggcagc ctcgatcgac aggtacggaa tgtccgtgtt gccgttgttg ctgaaggtga
     5101 gcccatacgt agcagagctg cccccccgga tggcgtccgg ggctgcgacc tgcgtgtcga
     5161 tggcgagacg cgtggcgggc tccacccgga accctcccgg aagcacggcc tcgccacccg
     5221 cgtcgttcac gacgaccacg tcataggt
//
LOCUS       DBNHQZ010000081         1903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3274286, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000081 DBNHQZ010000000
VERSION     DBNHQZ010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3274286"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="AC16YU_02055"
     CDS             complement(<1..1684)
                     /locus_tag="AC16YU_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064030.1"
                     /translation="MRTLYRIFPLMAYALVAAAFWPVTASGQIILDLRTEGAVADHNG
                     ARFIQEDASDGAGTGILDPFYRIKGNTGTVEGYNTGSPDVQFETESGPWTEALLLSTV
                     TDREVEGLGIFKEFLLDINQTAGNSIYAMVEVQVFEDDQPDLDCMDPVVPGSLATWGS
                     CDTGTLAEEIFWLDKPNDVSFPGESGNASVVMDYSLESGSGNQTDITMLIPYAAFSVG
                     EECAYGMPSASEVEGGCDTWVYVYSRFGIDERDTIDFSPNDDGFEEWSTIVHPFVNID
                     KTLAGSIDETHDWTLTKDVDSAVCDAFIGGSCTFDYDVNVVEIESRTVSIGGVVTYSV
                     PATLPDGDKAALDAEVTVLDDFYSDADGTIVWSDPSCAPGSPPFTLGAGDSKLCPYTI
                     TFADESLADGGTQTNTVTARVAITEVEVQATFEAVIAENITGPEEVDLSDTIQGDYGV
                     ISSSFLWQYTREIACSVNPEDYAGDGSVTQTIPNTASITDGLVLIDDDSESVTVNCYA
                     PVVSKTANASYTDEYAWTIEKSVSPEVHNLFAGETAGSDYDVNVVLEHNTYGY"
BASE COUNT          314 a          604 c          615 g          370 t
ORIGIN      
        1 catagccgta cgtgttgtgc tccaggacca cgttgacgtc atagtccgag cccgcagtct
       61 cacccgcgaa caggttgtgc acctcaggcg atacggactt ctcgatcgtc cacgcgtatt
      121 cgtccgtgta cgaggcgttg gccgtcttgc tgaccaccgg cgcgtagcag ttcacggtca
      181 cggactcgga gtcatcgtcg atgagcacca ggccgtccgt gatcgacgcg gtgttcggga
      241 tcgtctgcgt gactgatcca tcgccggcat agtcctccgg gttcacggag caggcgatct
      301 ctctcgtgta ctgccagagg aaggacgacg agatcacgcc gtagtccccc tggatcgtgt
      361 cggagaggtc gacctcctcc gggccggtga tgttctcggc gatgaccgcc tcgaacgtcg
      421 cctgcacttc cacctcggtg atggcaaccc gtgcggtcac cgtgttcgtc tgcgtgccgc
      481 cgtccgcgag ggattcgtcg gcgaacgtga tggtgtacgg gcagagcttg ctgtcgcccg
      541 ctccgagcgt gaacgggggc gaacccggag cgcaggacgg atccgaccat acgatcgtgc
      601 cgtccgcgtc ggagtagaag tcgtccagga ccgtcacctc ggcgtccagg gcggccttgt
      661 cgccgtcggg cagcgtcgcg ggaaccgagt aggtcaccac gccgccgatc gacacggtcc
      721 ggctctcgat ctccaccacg ttcacgtcgt aatcgaacgt gcagcttccg ccgatgaagg
      781 cgtcgcagac tgccgaatca acgtccttcg tgagcgtcca gtcgtgggtc tcgtcgatgc
      841 tgccggccag cgtcttgtcg atgttgacga acggatggac tatggtcgac cattcctcga
      901 aaccgtcgtc gttcggggag aagtcgatcg tgtcgcgctc gtcgatgccg aagcgggaat
      961 acacgtacac ccacgtgtcg catcccccct cgacctcgga ggcgctcggc attccgtacg
     1021 cgcactcctc gcccacggag aaggccgcgt acgggatcag catcgtgatg tccgtctggt
     1081 ttccgctgcc ggactccagg ctgtaatcca tcacgaccga tgcgttgccg gattcaccgg
     1141 ggaagctgac atcgttcggc ttgtccagcc agaagatctc ctcggcgagg gttccggtgt
     1201 cgcagctgcc ccacgtagcg aggcttccgg gcacgacagg gtccatgcag tcgaggtcgg
     1261 gctggtcgtc ctcgaatacc tgcacttcga ccatcgcgta aatcgagttg cccgccgtct
     1321 ggttgatgtc gagcaggaac tccttgaaga tgcccaggcc ctccacttcc cggtccgtga
     1381 cggtgctcag gagaagggcc tcggtccacg ggccgctctc cgtttcgaac tgcacgtcgg
     1441 gactacccgt gttgtagccc tcgaccgtcc cggtattgcc cttgatccgg tagaacgggt
     1501 cgaggatgcc ggttccggca ccgtcggagg cgtcctcctg gatgaagcgg gcgccgttat
     1561 gatcggctac ggcgccttcc gtcctcaaat cgaggatgat ctggccggac gccgtgacgg
     1621 gccagaaggc ggccgccacc agcgcatagg ccatcagtgg aaaaattcgg taaagcgttc
     1681 gcatgggggt tacctccccg ttaggtgatc cggtgcgcgg aaacacgacg gggccgatcg
     1741 caggcgccgg tcattaccgg ctgcgcatcg cgcaagacga ctcggtgggc agccatggca
     1801 ggtggggtgc cagggtggag gggtcccgcc gctcggccgt gccgcaggct gcagcttccg
     1861 gccggacagt gatgtgtttt catcataatc cgttggcggc gag
//
LOCUS       DBNHQZ010000082         4780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1920554, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000082 DBNHQZ010000000
VERSION     DBNHQZ010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1920554"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC16YU_02060"
     CDS             <1..351
                     /locus_tag="AC16YU_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064031.1"
                     /translation="PRVLGLFLMMPFLTVYADFIGILGGMIVATAFLDLSTTQFIGGM
                     LTAVSLGDALLGVFKGTVYGLIIGLAGCMRGLQTGKDAGAVGRSATSAVVTGITLIIV
                     ANALIDWLAVLLEI"
     gene            359..1141
                     /locus_tag="AC16YU_02065"
     CDS             359..1141
                     /locus_tag="AC16YU_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064032.1"
                     /translation="MESSAPIDVRDLTMAFGAKVVMRDLTFQVRPGEVFVIMGGSGSG
                     KSTLLRSLVGLLEPAAGTILFDGRDFWKADAEERKGMRRRMGVLYQGGALWSGLTLAE
                     NVALPIEEYTDLDPEAIRDVVSLKLALVGLRGFEPFYPNEISGGMRKRAALARATALD
                     PEVLFFDEPSSGLDPITASRLDDLILQLRASLGMTIVVVSHDLASIFRIADRALFLDI
                     EKKTMTALGDPEEMRKNPPSAEVHQFLTRSSDADPVPESNPR"
     gene            1147..2136
                     /locus_tag="AC16YU_02070"
     CDS             1147..2136
                     /locus_tag="AC16YU_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR6064033.1"
                     /translation="MSIRANPTAIGLFTIGAVALVVLGIAMLASGAWFSRWSTFVSYF
                     DESVNGLENGAPVKFQGVPVGRVTDLLIQIELADKTFQVPVQYEIELSRLTSQTGTFV
                     DLADPGVLRRQIANGLRAQLQMESMVTGLLYVELSYRTDAEPPVLEPGPTVYPEIPTT
                     QSLLAAFGTQAGSMVGDVLKILFRVNEMLESMEVEEMNAAIVSSAEALESLLESPEIR
                     DAFREVPVMSAQFNRTMAEMQILAERLGSAVEPVQANLDSTSAELIAALRTVRHGLEQ
                     MQGFFTTDSGIGYQMQEALAGLKDAADAMQILAFSLERNPDMLLRGKKPTLEH"
     gene            2152..2781
                     /locus_tag="AC16YU_02075"
     CDS             2152..2781
                     /locus_tag="AC16YU_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HIR6064034.1"
                     /translation="MKTARQITVIALVVLTGCFSLGRDEPVQIHYVLGGSGPAAIPAP
                     DSLVDDLTVALRQLQIAEYLQTPFIVVRREQHMITFSESNRWGERLREGITRTMAANL
                     AVRAPFRGVDTVPWPTGARHDYLIQLHVLLFEGAAPEDSLSSDGEARVTVSWEILGPR
                     DGSVLSRGITDFREDWTVGEYGVLVALLDTGLGVLADDLVNAMVDLAAP"
     gene            complement(2837..3532)
                     /locus_tag="AC16YU_02080"
     CDS             complement(2837..3532)
                     /locus_tag="AC16YU_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6064035.1"
                     /translation="MKYRIFILDDHPVMRRGYRTLINGEMDMEVCGEAETAMQALERI
                     PAAAPDLVIADITLEGINGIQFVKRLRTEQAGLGVLVVSMHEESLYAERALHAGAVGY
                     LMKTEADSSVIAAIRRVMGGKIYVSDRMQDKVMVVFAGRAPARDRPDSRSLVEKLSDR
                     ELEVFELHGRGLSTHDMAEALKISHKTVATHRGRIKTKLAIESTPELVRRAVLWIENE
                     KSATEAVESLNGE"
     gene            complement(3574..>4780)
                     /locus_tag="AC16YU_02085"
     CDS             complement(3574..>4780)
                     /locus_tag="AC16YU_02085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064036.1"
                     /translation="IDIGRPLEHLSHRLTYAGVINDARHSLESATEVEREVEANDGSW
                     FMMRVTPYQSARGPAGGVVLTFSDISSQKSAEQASVERFSLAFHAGPMAASIVTLDEG
                     RFLEVNQVFEQLTGYARDEVIGRLAGTLNLDFLREVEADPAGPERTDRLRNEEARIRT
                     MSGDLRDVIVATTAIEYEGRPCGLSHFYDVTERKRLEREVLQVSDREQRRIGADLHDG
                     LGTHLTGTALMARGLARNLRADRAVTAEEIDEIARLLTEGIAQARTLAHGLNPFQVEM
                     RGLANALKELALGLKDRAGIDCTFEETGRGKPLDGDSSMQLFRIVQEALTNAARHAQA
                     TRIRITLKRTGKHVRITIRDDGIGFEVETARATGMGLSTMRYRANIIGARLEIRSSPG
                     HGTTVTCSV"
BASE COUNT          798 a         1560 c         1564 g          858 t
ORIGIN      
        1 cccagggtcc tggggctctt cctgatgatg cccttcctga ccgtctatgc cgacttcatc
       61 gggattctgg gcgggatgat cgtggcgacc gcgttcctgg acctctccac gacccagttc
      121 atcggcggca tgctgacagc ggtgagcctg ggggatgcgc tgctgggcgt gttcaaggga
      181 acggtatacg gcctgatcat cgggctcgcg ggctgcatgc gcggtcttca gaccggaaag
      241 gacgccggcg cggtgggcag atcggctacc tcggccgtgg tcacgggcat cacgctgatc
      301 atcgtggcca atgccttgat cgactggctg gcagtcctgc tcgagatcta ggaaacccat
      361 ggaaagcagc gctcccatcg acgtacggga cctcacgatg gccttcggcg ccaaggtcgt
      421 catgcgggat ctgacgttcc aggttcggcc cggggaggtt ttcgtgatca tgggcgggag
      481 cggaagcggg aagagcacgc tgctccgaag cctcgtcgga ctcctggagc ccgcggccgg
      541 aaccatcctg ttcgacggtc gggatttctg gaaggccgac gcggaggaac ggaaagggat
      601 gcgccgccgc atgggggtcc tgtaccaggg tggcgccctg tggagcggat tgacgctcgc
      661 cgagaacgtg gctctgccca tcgaggaata cacggacctg gacccggagg cgatccggga
      721 cgtcgtttcg ctgaagcttg ccctcgtcgg gcttcgcggc ttcgagccgt tctacccgaa
      781 cgagatcagc ggcgggatgc ggaagcgcgc agccctggcc cgcgcgacgg ccctcgatcc
      841 cgaagtgctc ttcttcgacg aaccgtcgtc gggcctggac ccgattacgg caagccgcct
      901 ggacgacctg atcctccagc ttcgagccag cctgggcatg acgatcgtcg tggtcagcca
      961 cgacctcgcg agcatcttcc ggatcgccga tcgggcgctg ttcctggaca tcgagaagaa
     1021 gaccatgacc gcgctcggag acccggagga gatgcggaag aaccctccga gcgccgaagt
     1081 acaccagttc cttacacgca gcagcgacgc cgacccggta ccggaatcca acccacggtg
     1141 agacccatga gtatacgcgc caacccgacc gcgatcggac tgttcacgat cggagccgtc
     1201 gcgctggtgg tgctcggcat cgccatgctg gcttccggcg catggttcag caggtggtcg
     1261 accttcgtca gctatttcga cgagtcggtg aacggcctcg agaacggcgc accggtgaaa
     1321 ttccagggcg tgccggtggg cagggtcacg gatctgctga tccagatcga actggcggac
     1381 aagaccttcc aggtgccggt acaatacgag atcgagctca gccggctcac ctcgcagacc
     1441 ggcacgttcg tcgacctggc cgacccgggc gtcctgcggc ggcagatcgc caacggattg
     1501 cgggcacagc tgcagatgga gagcatggtg accggcttgc tctacgtcga gctctcgtac
     1561 cgcaccgatg ccgagccgcc ggttctcgaa cccggcccga cggtgtatcc ggagataccg
     1621 accacccagt cgctcctggc cgcgttcggg acgcaggccg gaagcatggt gggtgacgtg
     1681 ctgaagatcc tgttccgggt gaacgagatg ctcgaatcga tggaagtcga ggaaatgaac
     1741 gccgccatcg tctcctcggc cgaggcgctc gaaagcctcc tggaatcccc cgagatccgt
     1801 gatgccttcc gggaggtccc ggtgatgtcc gcgcagttca accggacaat ggccgagatg
     1861 cagatcctgg ccgagcggct cggctcggcc gtggagcccg tccaggccaa cctcgacagc
     1921 acgagcgccg agctgattgc cgcgctgcgg acggtgcggc atggtctcga acagatgcag
     1981 gggttcttca cgaccgattc cggtatcggc taccagatgc aggaggccct ggccggactc
     2041 aaggacgccg ccgacgccat gcagattctc gccttctcgc tggagcgcaa tccggacatg
     2101 cttcttcggg gcaagaagcc aaccctcgag cattagacac ggccaaacag gatgaagact
     2161 gcacgccaga ttaccgtaat cgccctggtc gttctgacgg ggtgtttcag cctgggccgt
     2221 gacgaaccgg tccagattca ctacgtactc ggcggaagcg ggccggccgc gattccggca
     2281 ccggactccc tcgtcgacga cctcaccgtc gcgctccgcc aactgcagat cgccgagtac
     2341 ctgcagacgc cgttcatcgt cgtgcggcgg gagcagcaca tgatcacctt ttccgagtcc
     2401 aaccggtggg gcgagagact tcgggagggg atcacccgga ccatggctgc caacctggct
     2461 gtccgggcac cattccgggg cgtggatacg gtgccgtggc cgacaggggc ccggcacgac
     2521 tacctgatcc agctccacgt cctgctcttc gaaggcgcgg cgcccgaaga ttcgttgtct
     2581 tccgacggag aggcccgcgt cacggtcagc tgggaaatcc tgggcccgcg ggacggctcc
     2641 gtgctgtcac gaggcatcac cgacttccgg gaggactgga cggtgggcga atacggcgtc
     2701 ctcgtggcac tgctcgatac cgggttgggc gtgctcgcgg atgacctggt caatgccatg
     2761 gtggaccttg ccgctccgta gcctgcgacg ggcgccccgg cgtcgatttc cgtccccggg
     2821 ccatccagca gcgcgctcac tctccgttga ggctctcgac cgcctccgtg gcggacttct
     2881 cgttctcgat ccagagcacc gcacgccgaa cgagttcggg agtgctctcg atggccagct
     2941 tggtctttat gcgaccccga tgcgtggcga ccgtcttgtg gctgatcttg agcgcctcgg
     3001 ccatgtcgtg ggtggagagt ccccggccgt gcagctcgaa aacctccagc tcgcgatcgg
     3061 acagcttctc cacgagggat cgcgaatcgg ggcgatcccg agcgggtgcg cgcccggcga
     3121 atacgaccat gaccttgtcc tgcatgcgat cgctgacgta gatcttcccg cccatgacgc
     3181 gccggatggc ggcgatgacc gaactgtcgg cctccgtctt catgaggtac ccgacggcgc
     3241 cggcgtgaag ggcacgctcc gcatagaggg attcctcgtg catggaaacg accaggacgc
     3301 ccaggcccgc ctgctcggtc cgcaggcgct tgacgaactg tatgccgttg atcccttcca
     3361 gggtgatatc ggcgatgacg aggtccgggg cggcggccgg aatcctctcc agggcctgca
     3421 tggcggtctc ggcctcaccg cacacctcca tgtccatctc cccgttgatc agcgtccggt
     3481 agccccgacg catgaccgga tggtcatcga gaatgaaaat ccggtatttc atggtaatcg
     3541 tgcgggtgga gcgacgccgg aggcgtcgat ggttcagacg gaacaggtga cggtcgtgcc
     3601 gtggcccggg gaactcctga tctcgaggcg ggcgccgatg atgttggcgc ggtagcgcat
     3661 ggtgctcagc cccatcccgg tggcgcgcgc cgtctccact tcgaagccga tgccgtcgtc
     3721 acggatcgtg atgcgcacgt gcttccccgt ccgtttcagg gtgattcgga tcctggtggc
     3781 ctgtgcgtgc cgggcggcat tggtgagcgc ctcctggacg attctgaaca gctgcatgct
     3841 cgaatcgccg tccagcggct ttccacgccc ggtttcctcg aacgtacagt cgatgcccgc
     3901 ccggtccttg aggccgagcg ccagctcttt cagggcgttc gccagccccc gcatttcgac
     3961 ctggaacggg ttgagcccgt gcgccagcgt gcgcgcctgc gcgatgcctt ccgtgagaag
     4021 tcgggcgatt tcgtcgattt cctcggcggt gaccgcacga tcggcccgga ggttgcgggc
     4081 aagacccctg gccatcaggg cggtgcccgt gaggtgggtt cccaggccgt cgtgcagatc
     4141 cgccccgatc cggcgctgct cacggtcgct cacctgcagg acctcgcgct ccaggcgctt
     4201 gcgctcggtc acgtcgtaga aatggctcag gccgcatgga cgaccctcgt actcgatggc
     4261 ggtggtggca acgatcacgt cgcgcagatc acctgacatg gtacggatgc gtgcttcctc
     4321 attcctcagg cggtccgtcc gttccgggcc cgcagggtcc gcctcgacct cgcgcaggaa
     4381 atccaggttc agcgtcccgg ccagccgacc gatgacttcg tcccgggcgt aaccggtaag
     4441 ctgctcgaat acctggttga cctccaggaa gcggccttcg tcgagcgtga cgatgctcgc
     4501 cgccatcgga cccgcatgaa aggccagcga gaaccgctcc acgctggcct gctccgcgct
     4561 cttctgcgac gagatgtcgg agaacgtgag tacgactccg ccggccggcc ccctcgccga
     4621 ctggtacggc gtcacgcgca tcatgaacca ggaaccgtca ttcgcctcga cctcgcgttc
     4681 cacctctgtc gcgctctcga gcgaatgcct ggcatcgttg atgacccccg cgtacgtcag
     4741 gcggtgggaa agatgctcga gagggcggcc gatgtcgatg
//
LOCUS       DBNHQZ010000083        18887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_294281, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000083 DBNHQZ010000000
VERSION     DBNHQZ010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 18887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18887
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_294281"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="AC16YU_02090"
     CDS             complement(<1..1031)
                     /locus_tag="AC16YU_02090"
                     /inference="COORDINATES: protein motif:HMM:NF042862.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon-like protease helical domain-containing
                     protein"
                     /protein_id="HIR6064037.1"
                     /translation="MPEFRLPDSLRPESLRTRCNPDVFDFETTADITDGPFVVGQERA
                     EEAFRFGIGMTQAGYNVYVVGDAGMGRRALVKRFLEQEAAERPVPDDWCYVNNFEDET
                     RPLALRLPPGRGATLRDGMARLIDDLGAALHAAFESEEYQLRRQVVQEAAREQEEEAL
                     QSLQTRAREQDIAVLRTPTGFAFAPLHDGEVISSKDLQGLPEEQQKKMQTAVTQFQEE
                     LQHILRQLPRRMRQLREELRTLNREVAEYAASELVAQLKDAFEDQPQVTDFIDRAVGE
                     MMKSIETAFLEPGDEDGGTARMAEVLDERTYGVNVLVDHTDASGAPIVHEDNPHYQGL
                     VGEVEQIAE"
     gene            complement(1127..2638)
                     /locus_tag="AC16YU_02095"
     CDS             complement(1127..2638)
                     /locus_tag="AC16YU_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6064038.1"
                     /translation="MSHPEINRRAFLKRASVATAAALLTPAALASDPYRPVYGFRPAA
                     RPVRIRGRVTVDGGGRRGVAVSDGRTVAVSGRDGSYELVSDGYRPFVFASVPAGAAIP
                     TSPVGTARFFHPIPPDGSDRLRADFAFRSPGRSDERHAFLALADTQTQNLFETGRLHA
                     ESVPDMQRTITGLGALPLFGIAVGDIMFDDLSLYPEYERAVERLGIPFLQVVGNHDLD
                     FDSPTDPGTTRTFRSHFGPTYYSFDRGQVHYVVLDDVFWHGAGYIGHLDADQLAWLAE
                     DLALVEPGRTVVVFQHIPALSTEYRRAGQARPGASVSVTNREALYRLLEPFEAHVLSG
                     HTHEHEHVFEGGVHEHVLGTVCGAWWSDDICHDGTPNGYAVVEVAGADLRWRYQATGR
                     PADERLRVYARGSVPDAPDEIVANVWDWDPRTTVTWTEDGVRRGAMARRTARDPWAVE
                     RFTGPERPDRRTWVEPGLVDHLFFAPVSEGARSVVVEWTDRFGAVWTASPPAP"
     gene            complement(2714..3865)
                     /locus_tag="AC16YU_02100"
     CDS             complement(2714..3865)
                     /locus_tag="AC16YU_02100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR6064039.1"
                     /translation="MKPEDYQYRRTGRYFALAAGNMEELAGGELAGLGAGSVEPAYRG
                     VHFDASDEVLYRVNYCSRLVTRVLAPVITFDCHSDRYLYRTAREIDWSELLSLEDTFA
                     VFATVSNSRITHSQFAARRLKDAIVDQFRDRTGRRPSVRTPDPDLWINLHIHGDRAVI
                     SLDTSGGSLHRRGYRKESVQAPMQETLAAAVVLLSGWDANRPLLDPMCGSGTLLAEAF
                     MHAARLPAAFGRSTFGFMRLPGYDAALWESVRSLADQAVRPVSEGLVTGSDRDPAAVK
                     AARRNLDSLPGGAAVRVETGAFERHPGISEGIVVCNPPYGVRLGTDAETPAVWTALGD
                     WLKQRCTGSSAFLYAGRPALLKSVGLRSTWKRALPNGALDGRLARYDLF"
     gene            complement(3862..4275)
                     /locus_tag="AC16YU_02105"
     CDS             complement(3862..4275)
                     /locus_tag="AC16YU_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064040.1"
                     /translation="MSQTPAPSRATLRIIRYAMLAGILVFGAVVAVLRSQGVLSDSGI
                     DGERSPVAIALSVMILATIGSVPLFSRLIRAAEDPVRRFQLVIAALAVAEAAALAGGV
                     LWLLSGTWTMYAVGVVLYLVAMIWVAPDAEAPGGP"
     gene            complement(4298..5521)
                     /locus_tag="AC16YU_02110"
     CDS             complement(4298..5521)
                     /locus_tag="AC16YU_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6064041.1"
                     /translation="MTSRAVRLAAVVLLLVSAGCGKRQLEPTVILVSIDGFHPDYQAR
                     FDTPVLDRLAREGVHAADGMLPVFPTKTFPNHYSIATGLYPARHGIVGNTMYDPGMDA
                     WFRLSDRDAVEDTRWWLGEPIWVTAEKQGVTAGTFFWPGTEAPVQGIQATYWKRFDNE
                     IPPGDRVDEVLRWLDLPDAERPRLVTLYFSDTDNVGHEHGPSSAEVGEAAERADGYLG
                     LLVSGLEERGLLDQVNLVVVSDHGMAEVSPDRVIVVEEIAELGESRVVEWSPVLLIEP
                     EPGRREAIEAALDTVRHLTRYDQAALDSLFRFAGSPRIPSIVAVAEAGWSITGNREFL
                     ESRRSRFPSGMHGFDPRDPSMAALFVARGPAFRSGYSAAPFENVHVYPLVAHILGLEP
                     ADVDGRLDAVRDLLR"
     gene            complement(5610..5891)
                     /locus_tag="AC16YU_02115"
     CDS             complement(5610..5891)
                     /locus_tag="AC16YU_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HIR6064042.1"
                     /translation="MKRTHRIASVALAVLGVTLLAGCGGPNLFERVGGFWGYGICGII
                     VVILDVLALLEIAGSSWPFGRKALWALLIIFFPVGGLILYWFFAREAGA"
     gene            6024..7103
                     /gene="dusB"
                     /locus_tag="AC16YU_02120"
     CDS             6024..7103
                     /gene="dusB"
                     /locus_tag="AC16YU_02120"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR6064043.1"
                     /translation="MRIGPFDFGDRPLFLAPMEDVSDPPFRLLCKRFGADLLYTEFIS
                     SGGLVHGADGSMRKLDFYEEERPLAVQIFGGDVEQVREAASIVDRAAPDIIDINFGCP
                     VKKVVCRDGGAGILRDLPKMRAITEAVVRASSRPVTVKTRLGWNDESIRILEVVRMLE
                     DAGVQALAVHARTRAQMYRGEARWDWLRRIKDEAGLRIPLIGNGDATTPGKIQDMFDE
                     TGVDAVMIGRGAIGNPWIFRDAKIFRETGDVPPGPSWEEIVGVVAEHLTLKCEWLGER
                     TGVLEMRRMYGGYFKGFRNASRLRTLLMDETARDGVLETLLNFREDEPDLQVAVAVRP
                     ARIEGAMRAALPTRATRRPLPEQAA"
     gene            7271..9580
                     /locus_tag="AC16YU_02125"
     CDS             7271..9580
                     /locus_tag="AC16YU_02125"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR6064044.1"
                     /translation="MDRLPRGILALLLCSAALPAAGQTVRTPPASRVQDPLPFRPNVR
                     PNLQVGRATAPITVDGELGDEGWAGSAVATSFSETYPGDQTVPPIGIEVWVTYDTENL
                     YLAYLIEDRPDRIRANMRDRDAIWQDDYVGVILDPYGDNAWAYFLAANPFGIQGDTKI
                     MNGGQEDVGFDVVYSSEGRITDSGYQVEVAIPFRSLRFPNAPTQEWKLTFWITHPRES
                     RSTYSWAAMSRDDPCSLCQLGSVSGIAGARPGGKVELLPAVTGSQSGTLVDRSAPTAG
                     LDNDRLTAEPSLGLKYSFTSNAIADLAVNPDFSQIEADAAQIDVNTTFALFFPERRPF
                     FQEGSDLFSTPIEQVYSRSINNPSAAAKFTGRFGRTSVGYIGGLDEDTPIILPFEDRS
                     MLAAAGNSYSNVLRYRRTLGTSSYVGGLVSDRRYLDDGGSGSTVGVDARITLTRALAV
                     EGQFVASRTVEQVDPGLLPAADGEIEFGAGHTAAFDGESFGGYAAYLSVEQQARHLFF
                     DLDFWRSNPTFRAANGFVTQNAINRSILVGGYRFYFEDSFVERLTPYFITGYWWNHDG
                     VRKDEFAWLSLEGRFKGQTNAQVQLLLVSNERFLGQDFRGLRRVEANVNSNFSDPVKL
                     GGGVSYGRSIARNLDVPEMGRALEIEAEGTFRPWSRLVVQPEVDYFRLSSEATGDELF
                     SGYILRTRVNYQFTRRLFLRLVTQYNDFGRRLDVDPLVTYKVNPFTAVYVGSTHDFVE
                     YPTGAGGPNEGFYQTDRQIFFKLQYLFRM"
     gene            complement(9675..10706)
                     /locus_tag="AC16YU_02130"
     CDS             complement(9675..10706)
                     /locus_tag="AC16YU_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR6064045.1"
                     /translation="MRKLIPLLLAAFLASCGGGSGERLVIYSPHGKEMLSEYERRFEA
                     RHPEVDVQWLDMGGQDAYDRIRTEKANPQADVWWGGDSPTFARAADEGLLAPYRPTWA
                     DAVPAAGRDTLDRWYATYRTPEVILYNDSTVKADERPTDWDDLLDEGWRGRIIIRYPL
                     ASSTMRTIWGALIMRQPTVEDGYAWLARLDMNTRTYAADPTQLYLKVARREGDVSLWN
                     MPDTEIQTEINGYPFDFAVPVSGTPLLYDGIAVVAGTDNPATARRFYEFVTSDSALVD
                     QARTYFRIPVRSDIEPELLPAWMRRPIPEMPIDWARLGEEGPTWMRYWDERIKGRGEA
                     WLRDSGRLP"
     gene            complement(10714..13059)
                     /locus_tag="AC16YU_02135"
     CDS             complement(10714..13059)
                     /locus_tag="AC16YU_02135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6064046.1"
                     /translation="MQQYHLVPEPAAGAPAPRIDADAVLSGLNEAQREAVLATDGPVL
                     IVAGPGSGKTRTLTHRIAYLLAMRKARPSELLAITFTNKAAREMRSRTDGLVGGDLAR
                     WMTIGTFHATLARVLRREAPALGFTSDFSIYDTDDTQRALRELMGRFNVDPKQHSPRA
                     IYGLISSAKNRMIGPSEYAKLAVGPAQEKAATLYAPYQELLRRSNAMDFDDLLLKPIE
                     LFRGHPDVLERYRDRWRFIHVDEYQDTNHAQYVVTRLLADGHRNLCVVGDDAQSIYAF
                     RGADIGNILSFQRDYPEATVVRLEQNYRSSKTIVRLADRIIRGNSRQIEKKLWTDNEE
                     GEPVLVLEALSEKDEAQKVERRIRDLCLRRGLRYRDVAVLYRTNAQSRSLEEAFRRED
                     VPYRIVGGVSFYQRREIKDAIAYLRLVVNPDDGGSLRRIINVPARGIGDRSQDLLFAF
                     AEREGIGPWEALHRLDEIPELGSRAVNALAGFRDLVAAHVREADAAPAGDLARSLIHQ
                     SGLVEDLMRENTPENLVRRENIAELVSAIQEFTENPDTPDTLSAFLQEVSLLTDADLG
                     GDDDDRVTLMTMHASKGLEFPAVFVTGLEEGLFPLSAAAQDPDELEEERRLFYVGTTR
                     AERHLVLAYARTRYRYGEQQSCLRSRFLDELDGDLLRNEAGGAFDPKRDRFALGGVPE
                     VRYDALDPHYYRRSLRPDLRPERSVTTVPKPPPGERRIVYDEEQTGTVAAGMRVEHSL
                     FGEGKVLAVEGDGPQAKATVFFPEVGQKKLILRFARLRRIG"
     gene            13226..14548
                     /locus_tag="AC16YU_02140"
     CDS             13226..14548
                     /locus_tag="AC16YU_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR6064047.1"
                     /translation="MTIESLLRGVLGIAVIIGIAFLVSNNRRRVNWRVVAAGLGLQLL
                     LAVFILKGSVMGEWFGPLEWPKQFFSWVSSFFVLVLNFTTAGARFVFGDLAIPQGADG
                     SLGFFFAFQVLPTIVFFGSLMAILYYLGIMQRVVQGMAWVVRRLLGTSGAESLSVTAN
                     IFVGQTEAPLVIKPYLEKMTYSELMAVMTGGMATIAGGVMAAYIQMLGDPYAAARGLS
                     LDVGRLLFAEQLLGASVMAAPAALMLAKIMIPETATPLTGGTVRVDAESGSSNVIDAA
                     ANGAVDGVKLAINVGGMLIAFLALIAMVNFILGWAGGLVGWELTLEQLLGWTVAPVAF
                     LIGVPWSDAVTFGSLLGTKVVVNEFVAYLSLAGEISAGALSPKSILMATFALCGFSNL
                     SSIAIQIGGIGPLAPSRTAELASLGLRAVLAGTLANMMTATIAGALTG"
     gene            complement(14570..15337)
                     /locus_tag="AC16YU_02145"
     CDS             complement(14570..15337)
                     /locus_tag="AC16YU_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6064048.1"
                     /translation="MIRLPTVRGVRIAVGAAALVTAGIAGAGCTTGPSIPLDAPEGWL
                     TDGRESWWLPDADTALAFRDLETLESMGLGIESPVYSADHLAESQVAVGRFRITEAVR
                     RDLLPLYRNHPEIVDSLFRRYVADNILPAPKTGDVRGLIQGYRRDAYRTLMRHFRAPA
                     TLVTLGRDIPVPVPDSLRAVAAGRSVSWQVFVGADGRPRALRLRSGIHPVLDRIALRA
                     VTQVTWQPAYVLRGGRSRPIDAWVRFGVRFPELPGPG"
     gene            complement(15334..15843)
                     /locus_tag="AC16YU_02150"
     CDS             complement(15334..15843)
                     /locus_tag="AC16YU_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6064049.1"
                     /translation="MELLIPIALVLAGLALIVAEVYLVPGFNVVGVLGLVLIVFAVGY
                     TFTESGFLGGMLTLVGTVVAGGSIFWFMWQSGAWDRFVLSTNLRQDEKVAASETEQRT
                     RFLGREGIALTPLRPTGVVEIGDERLEVMTEGEFIAAGSRIRIVAMDRRRYFVRLAET
                     VAREDVART"
     gene            complement(15872..17278)
                     /locus_tag="AC16YU_02155"
     CDS             complement(15872..17278)
                     /locus_tag="AC16YU_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6064050.1"
                     /translation="MQRTPLIRSLLVLAVLSLAAPRTGTAQPDSAWIPETGDGPVFVV
                     PVEGLIDNGLARYIDRALGDAEAAQAAAVIFHVDTFGGLVDAADQIRKAILNAPMPTI
                     AYIDKNAASAGALISYAADRIVMAPGSSIGAATVVEGMGGEAAPDKYQSYMRGLMRAT
                     AEANGRDPRIAEAMVDQDIEIEGISAAGQVLTLSASEALKFGVADAIEADLGAVTSAL
                     SAEGARLVEHEASGLERLLRFFGSPVVQSILMLMMLGGLYFELQTPGVGFPGLMAAVG
                     AVLFFGPSYMTGLAESWEMVLFAIGIVLLIVEIFVTPGFGVAGISGLVLVVGSLAVAL
                     IGNVGLSFPSGHAITQAVLTLAATMALLVVLAFSLGRYLPRSSRFGQLVLVPDLASAA
                     GYTSAEAREDLLGRTGIALTPLRPSGAAEIDGRRVDVTTAGEFIEARASVRVVSVHGS
                     RVEVRKVEPEPDPATSGA"
     gene            complement(17334..18092)
                     /locus_tag="AC16YU_02160"
     CDS             complement(17334..18092)
                     /locus_tag="AC16YU_02160"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6064051.1"
                     /translation="MTGCIRNGALLLAAVLLAPAGAHAQRSLPGEDALHYSIRSRSPV
                     FQLADSTSPYLTLGLQEPVQVLENGGGWARIRTRDGAQGLVRAEDLSNVWIRISKSRQ
                     SVLVYRGTELVDRIPADLGYNFFADKERRGSPGDPDHWRTPEGVFHVVEKNERSQYHR
                     ALVLNYPNEEDARRGLRDSLISEAQYWAILQANQSFRRPPMSTALGGWIEIHGRGTGQ
                     RANWTQGCVAIPDDAIDRIWPLVHVGTPVLIDPE"
     gene            complement(18089..18841)
                     /locus_tag="AC16YU_02165"
     CDS             complement(18089..18841)
                     /locus_tag="AC16YU_02165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6064052.1"
                     /translation="MIRLFVSDIDGCLSEPFRPFDRKRLDAIARLAARGDPWGVPGAP
                     PAFSLCTGRPYAYTEAMTQALGLTLPVLFEAGAGLFEPVKARTTWHPDFDEALERELD
                     ELRSWLASAVRGTGLFVDIGKRTQASVIGPARDDVRGMLPLVRARVAERHPDLTVAST
                     PVSIDILSRRLTKAQGLAWLADELRIPLGETAFIGDTDGDIVGLRAVGRSFAPANASE
                     EVRSVVDVVTTGRVAEGVHEAYLACIRTNQDP"
BASE COUNT         2873 a         6494 c         6673 g         2847 t
ORIGIN      
        1 atctccgcga tctgctcgac ctcgcccacg agtccctggt aatgggggtt gtcctcgtgc
       61 acgatcgggg cgccgctcgc gtccgtgtgg tcgacgagga cgttcacccc gtacgtgcgc
      121 tcgtccagca cctcggccat ccgggccgtc ccgccgtcct cgtcgcccgg ctcgaggaag
      181 gccgtctcga tcgacttcat catctcgccg acggccctgt cgatgaaatc ggtgacctgg
      241 ggctggtcct cgaacgcatc cttgagctgg gcgacgagct cggatgcggc gtattcggcc
      301 acctcacggt tcagcgtccg cagctcctcg cgcagctgac gcatgcggcg cgggagctgg
      361 cgcaggatgt gctgcagctc ctcctggaac tgcgtcacgg ccgtctgcat cttcttctgc
      421 tgctcctcgg gaaggccctg caggtccttg gacgagatca cctccccgtc gtgaagcggg
      481 gcgaacgcga acccggtggg ggtacgcagg acggcgatgt cctgctcgcg ggccctggtc
      541 tgcaggctct gcagcgcctc ctcctcctgc tcgcgggccg cctcctgcac gacctgacgg
      601 cggagctggt actcctcgct ctcgaacgcg gcgtgcaggg cggctccgag gtcgtcgatc
      661 aggcgcgcca tgccgtcgcg cagcgtcgcc ccgcggccgg gcgggagccg gagggcgagc
      721 ggccgcgtct cgtcctcgaa attgttgacg tagcaccagt cgtccgggac ggggcgctcg
      781 gcggcctcct gttcgaggaa ccgcttcacc agggcgcgtc gccccatgcc ggcgtccccg
      841 accacgtaca cgttgtagcc ggcctgcgtc atgccgatgc cgaaccggaa ggcctcctcg
      901 gcacgctcct gtccgacgac gaacggtccg tcggtgatgt cggccgtggt ctcgaagtcg
      961 aacacgtctg gattgcagcg tgtccggagg ctctcgggcc ggagcgaatc gggaaggcgg
     1021 aattcgggca tggcggagta tcgtttaccg ctcaaacgtg ccgccgccgc accgcaaaag
     1081 caagccggga ttcacccgga ctccgtgacg ggcgcatccg gccgggtcag ggagccggcg
     1141 gcgatgcagt ccacaccgcg ccgaaccggt ccgtccactc gaccaccacg ctacgggcgc
     1201 cctccgacac gggagcgaag aacaggtggt cgacgaggcc cggctccacc caggtcctac
     1261 gatccgggcg ctccgggccg gtgaaccgct ccacggccca cgggtcccgg gccgtgcgtc
     1321 gggccatcgc tccccggcgg acgccgtcct cggtccaggt gaccgtcgtc cgcgggtccc
     1381 agtcccacac gttggccacg atctcgtccg gggcgtcggg cacggatccc ctcgcgtaca
     1441 cccgcaggcg ctcgtcggcc ggccggccgg tcgcctggta gcgccagcgc aggtcggccc
     1501 cggcgacctc gacgaccgcg tagccgttcg gcgtcccgtc gtggcagatg tcgtcgctcc
     1561 accaggcgcc gcacaccgtg ccgagcacgt gctcgtgcac gccgccttcg aacacgtgct
     1621 cgtgctcgtg cgtgtggccg gataggacgt gcgcctcgaa gggctcgagc aggcggtaga
     1681 gggcctcccg gttggtaacc gagacggacg cgcctggccg cgcctggccg gcccgtcggt
     1741 actcggtcga tagggccgga atgtgctgga ataccacgac ggtccttccc ggctcgacca
     1801 gcgcgaggtc ctcggcgagc caggccagct ggtccgcgtc caggtgtccg atgtagcccg
     1861 ccccgtgcca gaagacgtcg tcgagcacga cgtagtgcac ctggccccgg tcgaaggaat
     1921 agtaggtggg gccgaaatgg ctccggaagg tccgggtcgt gcccgggtcc gtcggcgaat
     1981 cgaaatcgag gtcgtggttg ccgaccacct gcaggaaggg aatccccagg cgctcgaccg
     2041 cacgctcata ctcggggtag agcgacaggt cgtcgaacat gatgtccccc accgcgatgc
     2101 cgaagagcgg aagcgcgccc aggccggtga tcgtccgctg catgtccggc acggactcgg
     2161 cgtgcagccg gcccgtttcg aacaggttct gcgtctgcgt gtcggcgagc gcgaggaaag
     2221 cgtgccgctc gtcggaccgg cccggcgagc ggaaggcgaa gtccgcccgc aaccggtcgg
     2281 agccgtcggg cggaatcgga tggaagaagc gggccgtccc gaccggcgaa gtcggaatcg
     2341 ccgcacccgc cggcacggag gcgaagacga acggccggta gccgtcggag acgagctcgt
     2401 acgaaccgtc ccgtcccgac acggccaccg tcctcccgtc cgaaacggcc actccgcggc
     2461 gacccccgcc gtcgaccgtc acccgtcccc ggatccggac cgggcgcgcc gccggccgga
     2521 agccgtacac ggggcggtag ggatcggagg ccagggcggc cggggtcagc agggcggcgg
     2581 cggtggccac ggacgcccgc ttcaggaatg cccgccggtt gatctcggga tgggacatgg
     2641 ggccggtcgg atggtgctcg tcctcggagt atcgagggcg cggaccgccc gcgcaaccgc
     2701 tccgcgcgga ccttcagaaa agatcatacc gcgccagccg cccgtcgagc gcgccgttcg
     2761 ggagcgcccg cttccaggtc gagcgcaggc ccacggactt gagcagggcc ggacgcccgg
     2821 cgtacaggaa ggcgctcgat ccggtgcagc gctgcttcag ccagtcgccg agcgcggtcc
     2881 agacggcggg cgtctcggcg tcggtgccca ggcgcacgcc gtacggcggg ttgcagacga
     2941 cgatgccttc cgagatcccc ggatgccgct cgaacgctcc cgtctccacg cgcaccgccg
     3001 cgccgcccgg cagggaatcg aggttccggc gggcggcctt cacggcggcc gggtcccggt
     3061 cggaccccgt gacgagcccc tcggagaccg gcctcacggc ctggtcggcc aggcttcgca
     3121 ccgactccca gagggccgcg tcgtacccgg ggagccgcat gaacccgaac gtggaccggc
     3181 cgaacgcggc gggcagccgg gccgcgtgca tgaaggcctc cgcgagcagc gtgcccgagc
     3241 cgcacatggg gtccaggagg gggcggttcg cgtcccaccc ggagagcagg acgacggccg
     3301 cggcgagggt ctcctgcatg ggcgcctgga ccgattcctt ccggtacccg cgccggtgaa
     3361 gcgagccgcc cgaggtgtcc aggctgatca cggcccggtc gccgtggata tgcaggttga
     3421 tccagaggtc gggatcgggc gtgcggacgg acgggcgacg ccccgtgcga tcgcggaact
     3481 ggtccacgat ggcgtccttg agccggcggg ccgcgaactg cgagtgcgtg atccggctgt
     3541 tggacaccgt agcgaacacg gcgaacgtgt cctcgagcga gagcagttcc gaccagtcga
     3601 tctcccgggc cgtgcggtac aggtaccggt cgctgtggca gtcgaaggtg atgaccggcg
     3661 cgaggacgcg cgtgacgagc cgggagcagt agttgacgcg gtacaggacc tcgtccgatg
     3721 catcgaaatg gacgccgcgg taggcgggct ccacggaacc ggcgccgagc ccggcgagct
     3781 ccccgccggc aagctcctcc atgttgccgg ccgccagggc gaagtagcgc ccggtccgcc
     3841 gatactggta gtcttccggc ttcacggtcc gcccggcgct tccgcgtcgg gagcgaccca
     3901 gatcatggcg accaggtaca ggacgacccc gacggcgtac atcgtccagg tgccgctcag
     3961 cagccacagg acgccgccgg ccagggccgc cgcttcggcc accgccaggg cggcgatcac
     4021 gagctggaag cgccgaaccg ggtcttcggc cgcccggatg agccgggaaa agagcggcac
     4081 ggacccgatc gtcgcgagga tcatcacgct gagcgcgatc gcgacgggcg agcgctcgcc
     4141 gtcgatcccc gagtcggaaa ggactccctg gctgcgcagg acggccacga ccgcgccgaa
     4201 caccaggatg cctgcgagca tggcgtaccg gatgatccgg agggtggcgc gggaaggtgc
     4261 cggcgtctgg ctcatggcgg ctcgggctgc ggcctcgcta acggagcagg tcccgcacgg
     4321 cgtcgaggcg tccgtcgacg tcggcgggct ccaggcccag gatatgcgcg accaggggat
     4381 acacgtggac gttctcgaac ggcgcggccg agtacccgga ccggaaggct ggcccccggg
     4441 cgacgaacag ggccgccatc gacggatccc tcgggtcgaa gccgtgcatg ccggagggga
     4501 agcgcgatcg cctggactcc aggaactcac ggttgccggt gatcgaccat cccgcctcgg
     4561 cgacggcgac gatcgacggg atgcgcgggc ttccggcaaa acggaacagc gaatcgagcg
     4621 cggcctggtc gtaccgggtc aggtgccgca cggtgtccag cgcggcctcg atcgcctcgc
     4681 ggcggccggg ctcgggctcg atcaggagca ccggagacca ttcgacgacc cggctctcgc
     4741 ccagctccgc gatctcctcg acgacgatca cgcgatccgg cgacacctcg gccatgccgt
     4801 ggtcggaaac cacgacgagg ttcacctggt ccaggagtcc ccgctcctcc aggcccgaga
     4861 ccaggaggcc caggtagccg tccgcccgct cggcggcctc gcccacctcg gcgctcgacg
     4921 gcccgtgctc gtgtccgacg ttgtccgtgt ccgagaaata gagggtcacg agcctgggcc
     4981 tctcggcgtc cggaaggtcc agccagcgga gtacctcgtc cacccgatcc ccgggaggaa
     5041 tctcgttgtc gaaccgcttc cagtacgtgg cctggatgcc ctggacgggc gcctcggtcc
     5101 ccggccagaa gaaggtgccg gccgtcacgc cctgcttctc ggcggtcacc cagatgggct
     5161 ccccgagcca ccagcgcgtg tcctcgaccg catcgcggtc gctcagcctg aaccacgcgt
     5221 ccatgcccgg atcgtacatc gtgttgccga cgatgccgtg gcgggccggg tacaggccgg
     5281 tcgcgatcga gtagtggttc ggaaaggtct tcgtggggaa gaccggcagc atgccgtccg
     5341 ccgcgtggac gccctcgcgg gcaagccggt ccaggacggg cgtgtcgaac cgggcctggt
     5401 agtcgggatg gaacccgtcg atcgagacca ggatgacggt gggttcgagt tgccgcttcc
     5461 cgcatccggc ggatacgagc agcagcacga cggccgcgag gcggaccgcg cgagaagtca
     5521 tgggcgaggg aggaatggtg acgcgcgccc caaaatagag gcggccccgt cacccacgcg
     5581 ccgtccgccg tgaaaaggcg ggcaatccgt caggcgcccg cttcccgtgc gaagaaccag
     5641 tacaggatca ggccgccgac cgggaagaag atgatcagca gcgcccagag ggccttgcgg
     5701 ccaaacggcc agctggagcc cgcgatctcg aggagcgcga ggacgtccag gatgacgacg
     5761 atgatgccgc agatgccgta tccccagaag ccgcccacgc gctcgaacag gttgggtcct
     5821 ccacacccgg cgagaagcgt gacgccgagg acggcgaggg cgaccgaagc gatgcgatgc
     5881 gtgcgtttca tggagtcggg tcggatgtcg aaggtcgcct cctgtatacg aaggaacggg
     5941 aaaaggttgg gcacggcccg cgtgcccgcc cggaactggc ccgcgaggga cctcgttcga
     6001 ggcggcggca cacgaagggc cccatgcgca tcggaccgtt cgatttcggc gaccgccccc
     6061 tgtttctcgc gcccatggag gacgtgagcg acccaccgtt ccggctgctg tgcaagcggt
     6121 tcggggccga cctgctgtac accgagttca tctcgtcggg cgggctggtg cacggggcgg
     6181 acgggtccat gcggaagctg gacttctacg aggaggagcg gccactggcc gtgcagatct
     6241 tcggaggcga cgtggagcag gtccgcgagg cggcctcgat cgtggaccgc gcggcaccgg
     6301 acatcatcga catcaacttc ggctgtcccg tgaagaaggt ggtgtgccgg gacgggggag
     6361 cgggcatcct tcgtgacctg cccaagatgc gggcgatcac cgaggccgtg gtgcgggcgt
     6421 cgtcgcgccc cgtcaccgtg aagacgcgcc tggggtggaa cgacgagtcg atccggatcc
     6481 tggaggtcgt ccggatgctc gaggacgcgg gcgtccaggc cctggccgtc cacgcgcgca
     6541 cgcgcgccca gatgtaccgg ggcgaggcgc gttgggactg gctgcggcgg atcaaggacg
     6601 aggcgggcct ccgcattccg ctcatcggca acggggatgc gaccaccccg gggaagatcc
     6661 aggacatgtt cgacgagacc ggggtcgatg ccgtgatgat cgggcgggga gccatcggca
     6721 acccgtggat cttccgcgac gcgaagatct tccgcgaaac gggcgacgtg ccgcccggcc
     6781 cgtcctggga ggaaatcgtg ggcgtggttg cggagcatct cacgctcaag tgcgagtggc
     6841 tcggcgaacg gaccggcgtc ctggagatgc ggcggatgta cggcggctac ttcaagggat
     6901 tccggaacgc gtcgcggctg cgcacgctgc tcatggacga gactgcgcgg gacggcgtcc
     6961 tcgagacgct gctcaatttc cgggaggacg agcccgacct gcaggtcgcc gtggccgtcc
     7021 gaccggcgcg gatcgagggt gcgatgcggg ccgctctccc gacgcgggcc acgcggcgtc
     7081 cccttccgga acaggccgcc tgacgccgac ggcccgtcgg ggcataaaag tgcccctgcg
     7141 cgtcggcgtg ccccgggcgc tgtaaccggg ccgcccggtc cccgtaggcg accgggtatc
     7201 tgccgaagcc gcatcggagc tcgtacagcg gcttcgccag gggatccccg cccaccgacc
     7261 ggtcgccccc atggatcgtc ttccgcgcgg cattctggcg ctgctcctct gttcggccgc
     7321 attgccggcc gcgggtcaga cggttcggac gccgcccgcc tcccgtgtcc aggaccccct
     7381 gccgttccgc cccaacgtgc ggccgaacct gcaggtcgga agggcgaccg cgccgatcac
     7441 ggtggacggg gagctgggcg acgagggctg ggccggctcc gcggttgcga cgtccttctc
     7501 ggagacgtat cccggggacc agaccgttcc gcccatcggc atcgaggtct gggtgacgta
     7561 cgatacggag aacctgtacc tggcctacct gatcgaggac cgcccggacc gcatccgggc
     7621 gaacatgcgc gaccgggacg ccatctggca ggacgactac gtgggggtga tcctggaccc
     7681 gtacggggac aacgcgtggg cgtacttcct cgccgccaac cccttcggca tccagggaga
     7741 cacgaagatc atgaacggag gtcaggagga cgtgggtttc gacgtggttt actcatccga
     7801 gggcaggatc accgactcag gataccaggt cgaggtggcc atcccgttcc gcagcctccg
     7861 gtttccgaac gcgccgacgc aggagtggaa gctgacgttc tggatcacgc acccgcgcga
     7921 gagccgctcc acgtattcct gggcggccat gagccgggac gatccctgct cgctgtgcca
     7981 gctcggctcg gtgtccggga tcgccggcgc gaggccgggc ggcaaggtcg aactgctgcc
     8041 cgccgtcacc ggaagccaat cgggcacgct cgtcgaccgg agtgcgccca ccgccggcct
     8101 ggacaacgac cgcctgacgg ccgagccctc gctcggcctc aagtactcgt tcacgtcgaa
     8161 cgccatcgcg gacctggcgg tcaatcccga cttcagccag atcgaggcgg acgccgcgca
     8221 gatcgacgta aacacgacgt ttgcgctgtt ctttccggaa cgcaggccgt tcttccagga
     8281 gggttcggac ctgttctcga cgccgatcga gcaggtctat tcgcgatcga tcaacaatcc
     8341 gtcggccgcg gccaagttca cgggccggtt cggacggacg agcgtcggct acatcggcgg
     8401 gctggacgag gacacgccca tcatcctccc gttcgaggat cggtccatgc tggcggcggc
     8461 cgggaacagc tacagcaacg tgctccgcta ccgccggacc ctggggacga gctcgtacgt
     8521 gggcggcctg gtcagcgacc ggcggtacct cgatgacggc ggctccggat cgaccgtcgg
     8581 cgtggacgcc cggatcacgc tcacgcgcgc gctcgcggtg gaaggacagt tcgtggcgag
     8641 ccggaccgtc gagcaggtcg atcccggcct gcttcctgcc gcggacggcg agatcgagtt
     8701 cggcgccggg cacacggccg ccttcgacgg cgagtcgttc ggcggctacg cggcctactt
     8761 gagcgtagag cagcaggcgc ggcacctgtt cttcgacctg gatttctgga ggtcgaaccc
     8821 gacgttccgt gccgcgaacg ggttcgtcac ccagaacgcc atcaaccgct cgattctcgt
     8881 cggcggatac cgcttctatt tcgaggattc gttcgtggag cgcctgacgc cgtacttcat
     8941 caccgggtac tggtggaacc acgacggcgt ccggaaggac gagttcgcct ggctgtccct
     9001 ggagggccgg ttcaaggggc agaccaacgc ccaggtccag cttctgctcg tgagcaacga
     9061 gcgcttcctg gggcaggact tccgcggact gcgacgggtg gaggcgaacg tgaactcgaa
     9121 cttctccgac ccggtgaagc tgggcggagg cgtcagctac gggcgatcca tcgcacgcaa
     9181 cctggacgtg cccgagatgg ggcgcgccct cgagatcgag gccgagggca cgttccggcc
     9241 ctggtcgcgg ctcgtcgtcc agcccgaggt ggactacttc cggctcagct ccgaggcgac
     9301 gggagacgag cttttctcgg gctacatcct gcgcacccgc gtgaactacc agttcacccg
     9361 ccggctcttc ctgcgcctcg tcacccagta caacgacttc gggcgccggc tggacgtgga
     9421 tccgctcgtt acgtacaagg tgaatccgtt cacggccgtg tacgtgggct ccacccacga
     9481 tttcgtggag tatcccacgg gcgcaggcgg tccgaacgaa ggcttctacc agacggaccg
     9541 ccagatcttc ttcaagctgc agtacctctt ccgcatgtag ggaagccgga ttgcaccgtg
     9601 cggctcgtca tctgagggag ggacgatccg cgacgtggcc gggacctcga agccgtcggg
     9661 gtcccggccg ccgttcaggg caggcgcccc gaatccctga gccaggcctc gccccgtccc
     9721 ttgatgcgct catcccagta gcgcatccat gtcgggcctt cctcgcccag gcgggcccag
     9781 tcgatcggca tctccgggat cggccgccgc atccacgcgg gcaggagttc gggctcgatg
     9841 tccgaccgga ccgggatgcg gaagtacgtg cgtgcctggt ccacgagcgc cgagtcggac
     9901 gtgacgaact cgtagaagcg gcgggccgtg gccgggttgt ccgtgccggc cacgaccgcg
     9961 atgccgtcgt agagaagggg cgtgccggag accgggaccg cgaaatcgaa cgggtatccg
    10021 ttgatctcgg tctgtatctc ggtgtccggc atgttccaca gcgagacgtc cccctcgcgg
    10081 cgggcgacct tcaggtacag ctgcgtcggg tcggccgcgt acgtgcgggt gttcatgtcc
    10141 agccgcgcga gccaggcgta cccgtcctcg accgtcggct gccgcatgat gagagctccc
    10201 cagatcgtgc gcatcgtgct ggatgcgagc gggtaccgga tgatgatccg cccgcgccag
    10261 ccctcgtcca ggaggtcgtc ccagtccgtg ggccgctcgt cggccttcac ggtggagtcg
    10321 ttgtacagaa tgacctccgg cgtccggtac gtggcatacc agcggtcgag cgtgtcgcgt
    10381 ccggcagcgg gcacggcgtc cgcccatgtg gggcggtacg gggcgagcag tccctcgtcc
    10441 gcagcgcggg cgaaggtcgg gctgtcgccg ccccaccaga cgtcggcctg cggattggcc
    10501 ttctccgtgc ggatgcggtc gtaggcgtcc tggccgccca tgtccagcca ctgcacgtcg
    10561 acctcggggt gtcgcgcctc gaagcgccgc tcgtactcgg agagcatctc cttgccgtgc
    10621 ggcgagtaga tcaccagccg ctctccggag ccgccgccgc acgaggccag gaaggccgcg
    10681 agaaggaggg gaatcagctt gcgcatggtg atgtcagcct atgcggcgca gcctggcgaa
    10741 ccgcaggatg agtttcttct ggccgacctc ggggaagaac accgtggcct tggcctgcgg
    10801 cccgtcgccc tccacggcga gcaccttgcc ttcgccgaac agggagtgct cgacccgcat
    10861 tcccgcggcc accgtgccag tctgctcctc gtcgtacacg atgcgccgct cgcccggcgg
    10921 cggcttgggc acggtcgtga cgctccgctc ggggcgaagg tccggccgga ggctccgccg
    10981 gtagtagtgc ggatccaagg cgtcgtagcg gacctcgggc acgccaccga gcgcgaatcg
    11041 gtcgcgcttc ggatcgaacg cccccccggc ctcgttgcgc agcaggtccc cgtcgagctc
    11101 gtccaggaag cgggaccgga ggcacgactg ctgctcgccg taccggtacc gcgtgcgggc
    11161 gtaggcgagc accaggtggc gctcggcgcg cgtcgtcccc acgtagaaca gccgccgctc
    11221 ctcctcgagc tcgtccggat cctgcgcggc cgcgctgagc ggaaagagcc cctcctccag
    11281 gcccgtcacg aagacggccg ggaattccag tcccttggac gcgtgcatgg tcatgagcgt
    11341 cacgcggtcg tcgtcgtcgc cacccaggtc cgcgtccgtc aggagcgaga cctcctgcag
    11401 gaaggcgctc agcgtgtcgg gggtgtccgg gttctcggtg aactcctgga tcgccgacac
    11461 gagctccgcg atgttctcgc gccggaccag gttctccggg gtattctccc gcatcaggtc
    11521 ctcgacgagc cccgactggt ggatcaggct tcgcgccagg tcgccggccg gcgcggcgtc
    11581 ggcctcccgc acgtgcgccg cgacgagatc gcggaacccc gcgagggcgt tcacggcacg
    11641 ggagcccagt tccgggatct cgtccaggcg gtgcagcgct tcccagggcc cgatgccttc
    11701 ccgttcggcg aacgcgaaga gcaggtcctg cgagcggtcg ccgatcccgc gcgcgggcac
    11761 gttgatgatc cgccgcaggc tgcccccgtc gtcggggttg accacgagcc tcaggtaggc
    11821 gatcgcgtcc ttgatttcgc gccgctggta gaatgacacc ccgcccacga tccggtacgg
    11881 cacgtcctcg cggcggaacg cctcctcgag cgagcggctc tgggcgttgg tccggtaaag
    11941 gacggccacg tcgcggtagc ggagaccccg gcgcaggcac aggtcgcgga tgcgccgctc
    12001 caccttctgt gcctcgtcct tctccgagag cgcctccagc accaggaccg gctcgccctc
    12061 ctcgttgtcc gtccagagct ttttctcgat ctggcggctg ttgccccgga tgatccggtc
    12121 ggccaggcgg acgatggtct tcgacgagcg atagttctgc tcgaggcgaa cgacggtggc
    12181 ttcggggtag tcgcgctgga aggagaggat gttgccgatg tccgcccccc ggaatgcgta
    12241 gatcgactgc gcgtcgtccc ccaccacgca caggttccgg tggccgtcgg cgagaagccg
    12301 ggtcacgacg tactgggcgt ggttcgtgtc ctggtactcg tccacgtgga tgaagcgcca
    12361 ccggtcccgg taccgctcga gcacgtccgg atgaccgcgg aacagctcga tcggcttcag
    12421 gagcaggtcg tcgaagtcca tcgcgttgga tcgccggagt agctcctggt acggggcgta
    12481 gagcgtggcg gccttctcct gggccggccc cacggcgagc ttagcgtact cggacggccc
    12541 gatcatgcgg ttcttggccg acgagatcag gccgtagatg gcccgggggc tgtgctgctt
    12601 cgggtcgacg ttgaaccggc ccatgagctc gcggagcgcg cgctgcgtgt cgtccgtgtc
    12661 gtagatcgag aagtcggacg tgaagccgag cgccggcgcc tcgcggcgca ggacgcgggc
    12721 gagcgtcgcg tggaaggtcc cgatcgtcat ccagcgggcg aggtcgccgc ccacgaggcc
    12781 gtcggtccgg gagcgcatct cccgggccgc cttgttcgtg aaggtgatcg cgagcagctc
    12841 ggagggacgg gccttgcgca tcgcgagcag gtaggcgatg cggtgcgtga gggtccgggt
    12901 ctttcccgac ccgggaccgg ccacgatcag cacgggaccg tcggtcgcca ggacggcctc
    12961 ccgctgcgcc tcgttcaggc ccgagagcac cgcgtccgcg tcgatgcggg gcgcgggcgc
    13021 gcccgcagcg ggctccggga cgaggtgata ctgctgcatg gatcgggggg ccggatgggg
    13081 cccgtcgtac acgcggaacc gggggagtcg tttcccccgg cgggacgctg ttccgccggc
    13141 ccgctgcggt cgcgcgacga ccggggcgcg tatattcgcg ccggccgaac gatgtcgcgc
    13201 ttcccctggc acatcccgca accccatgac gatcgaatcc ctgctccggg gcgttctggg
    13261 catcgccgtc atcatcggta tcgcgttcct ggtctcgaac aaccgcaggc gggtgaactg
    13321 gagagtcgtg gccgccgggc tcggcctgca gctcctgctc gcggtgttca tcctgaaggg
    13381 ctcggtgatg ggggagtggt tcgggccgct ggagtggccc aagcagttct tctcgtgggt
    13441 gagctccttc ttcgtcctgg tcctgaactt cacgacggct ggagcgcggt tcgtcttcgg
    13501 cgacctcgcg attccgcagg gcgcggacgg cagcctgggc ttcttcttcg cgttccaggt
    13561 ccttccgacg atcgtgttct tcggatccct catggcgatc ctgtactacc tgggcatcat
    13621 gcagcgggtg gtgcagggca tggcctgggt ggtgcggcgt ctgctcggca cctccggcgc
    13681 ggagtcgctc tcggtcacgg cgaacatctt cgtcgggcag accgaggcgc cgctggtcat
    13741 caagccctac ctggagaaga tgacctactc cgagctcatg gccgtcatga cgggcggcat
    13801 ggccacgatc gcgggcggcg tgatggcggc ctacatccag atgctgggcg atccgtacgc
    13861 ggcggcccgg ggcctttcgc tggacgtggg caggctgctc ttcgcggagc agctcctcgg
    13921 cgcgagcgtg atggccgccc cggccgccct catgctggcc aagatcatga ttcccgagac
    13981 ggcgacgccg ctcacgggcg gcaccgtgcg ggtcgacgcc gagtcgggat cgtccaacgt
    14041 gatcgacgcg gccgcgaacg gagcggtgga cggcgtgaag ctcgcgatca acgtgggcgg
    14101 catgctgatc gccttcctgg cgctcatcgc gatggtcaat ttcatcctgg ggtgggccgg
    14161 cgggctggtc ggatgggagc tgacgctcga gcagctcctg ggctggaccg tggcgccggt
    14221 cgccttcctg atcggcgtgc cgtggagcga cgcggtgacg ttcggctccc tgctcggcac
    14281 caaggtcgtc gtgaacgagt tcgtcgccta cctgagcctc gcgggcgaga tctccgcggg
    14341 cgcgctctcg ccgaagtcga tcctcatggc cacgttcgcg ctctgcgggt tctcgaacct
    14401 gtcgtcgatc gcgatccaga tcggcggtat cggcccgctg gccccgtcgc gcaccgcgga
    14461 gctcgcgtcg ctcggcctga gggcggtgct ggcgggaacg ctcgccaaca tgatgacggc
    14521 caccatcgcg ggcgcgctca ccggctgacc gggcccgccg cgcggcgcct cagcccggcc
    14581 ccggcagctc ggggaaccgg acgccgaagc gcacccaggc atcgatcggg cgggaccggc
    14641 cgccgcggag cacgtaggcc ggctgccagg tcacctgcgt aaccgcccgg agggcgatgc
    14701 ggtccaggac gggatggatg cccgagcgga gccggagcgc gcgcggccgc ccgtccgctc
    14761 cgacgaacac ctgccaggag accgaacgcc ccgccgcgac cgcacggagc gagtccggaa
    14821 cggggaccgg gatgtcgcgg ccgagcgtga cgagggtcgc gggcgcgcgg aagtggcgca
    14881 tcagggtccg gtacgcgtcg cggcggtagc cctggatcag gccgcgaacg tcgcccgttt
    14941 tcggcgccgg caggatgttg tccgcgacgt agcgccggaa cagggagtcg acgatctccg
    15001 ggtggttccg gtacaggggg agcaggtcac ggcggacggc ctccgtgatc cggaaccggc
    15061 cgacggccac ctggctctcg gcgagatggt cggccgagta gacgggcgac tcgatcccga
    15121 gccccatcga ttcgagcgtc tccaggtccc ggaaggcgag cgcggtatcc gcgtccggca
    15181 gccaccagga ttcgcgcccg tccgtcagcc agccctccgg agcatccagc gggatggacg
    15241 gcccggtcgt gcaccccgcc ccggcgattc cggccgtgac gagcgcggcg gcaccgaccg
    15301 cgatccggac gccccgcacg gtcggaaggc ggatcacgtc cgggctacgt cttccctggc
    15361 cacggtctcc gcgagccgga cgaaataccg gcggcggtcc atggcgacga tccggatgcg
    15421 gcttccggcc gcgatgaatt ctccctcggt catcacctcg agccgctcgt cgccgatctc
    15481 cacgactccc gtgggccgga gcggcgtgag ggcaatgcct tcgcgaccga ggaaccgggt
    15541 ccgctgctcg gtctcgctcg ccgcgacctt ctcgtcctgc cggagattgg tcgacagcac
    15601 gaagcggtcc catgccccgg actgccacat gaaccagaat atcgatcctc cggcgacgac
    15661 cgtccctacg agcgtaagca tgccgcccag gaagccgctc tcggtgaacg tgtacccgac
    15721 ggcgaacacg atcagcacga gcccgagaac gcccacgacg ttgaatcccg gaaccaggta
    15781 tacctcggcc acgatcagcg cgaggccggc caggacgagg gctatgggga tcagcagctc
    15841 caaggggaca gggggaccac gattctacgg atcacgcgcc ggatgttgca ggatccggtt
    15901 ccggctcgac cttccggacc tcgacccggc ttccgtgcac gctgaccacc cggacggagg
    15961 ccctggcctc gatgaactcg cctgccgtgg tcacgtccac ccggcgcccg tcgatttcgg
    16021 ccgcgcccga ggggcggagc ggcgtgagcg cgatgccggt gcgcccgaga agatcctcgc
    16081 gcgcctccgc cgacgtgtag ccggcggcgc tggccaggtc gggcaccagc acgagctggc
    16141 cgaaccggga ggaccgcggc aggtatcgac cgagcgagaa ggccaggacc acgagcagag
    16201 ccatggtcgc cgcgagcgtg aggaccgcct gcgtgatcgc gtggcccgac gggaacgaga
    16261 gaccgacgtt tccgatcagg gccacggcga gcgagcccac cacgagaacg agtcccgaga
    16321 tgcccgccac gccgaatccc ggcgtgacga agatttccac gatgagcagc acgatgccga
    16381 tcgcgaagag gaccatctcc cacgactcgg cgaggcccgt catgtagctc gggccgaaga
    16441 agagcacggc cccgacggcc gccatgaggc ccgggaagcc cacgccgggc gtctgcagct
    16501 cgaaatacag gcccccgagc atcatgagca tcaggatcga ctgcacgacg ggcgacccga
    16561 agaagcggag cagccgctcc aggcccgaag cctcgtgctc cacgagccgg gctccctcgg
    16621 ccgacagcgc cgaggtcacc gcgccgaggt cggcctcgat ggcgtccgcg acgccgaact
    16681 tgagcgcctc ggaggcggaa agcgtgagca cctgtcccgc ggcggatatg ccctcgatct
    16741 cgatgtcctg gtccaccatg gcctcggcga tccgcgggtc gcgaccattg gcctcggccg
    16801 tcgcccgcat cagcccgcgc atgtagctct ggtacttgtc gggagccgcc tctccgccca
    16861 tgccctccac gacggtcgcg gcaccgatgg acgatcccgg tgccatgacg atccggtcgg
    16921 ccgcgtacga gatcagcgct ccggcggacg ccgcattctt gtcgatgtac gcgatcgtgg
    16981 gcatcggggc gttaaggatg gccttccgga tctggtccgc cgcatcgacg agcccgccga
    17041 acgtatccac gtggaagatc accgccgccg cctgcgcggc ttccgcatct ccgagggcgc
    17101 ggtcgatgta gcgggcgagc ccgttgtcga tcaggccctc cacgggaacg acgaagaccg
    17161 gtccgtcccc cgtctcgggg atccaggcgc tgtcgggctg tgccgtgccc gtgcgcggcg
    17221 ccgcgaggct caggacggcc agaacgagca gcgaccgtat gagaggtgtc cgctgcatcg
    17281 gcgtacggcg gttgggttcc atccggttcg tacgccggat ccgtgaccgt gattcactcc
    17341 gggtcgatga ggaccggcgt cccgacatgc accaggggcc atatgcggtc gatcgcgtcg
    17401 tccggaatgg cgacgcatcc ctgggtccag ttcgcccgct gcccggtgcc ccggccgtgg
    17461 atctcgatcc agccgccgag ggcggtgctc atcgggggcc tcctgaacga ctggttggcc
    17521 tgcaggatgg cccagtactg cgcctccgag atgaggctgt cgcgaaggcc ccgcctggcg
    17581 tcctcctcgt tcggatagtt caggaccagg gcacgatggt actggctgcg ctcgttcttc
    17641 tcgaccacgt ggaagacgcc ttccggcgtc cgccagtggt cggggtcgcc cgggctgccc
    17701 cgccgttcct tgtccgcgaa gaagttgtag ccgaggtccg ccggaatgcg gtccacgagc
    17761 tcggtaccgc ggtagacgag gaccgactgg cgggacttcg agatccggat ccagacgttg
    17821 gagaggtcct cggcacgcac caggccctgg gcgccgtcgc gggtcctgat ccgcgcccag
    17881 ccgcccccgt tctcgaggac ctggacgggc tcctgcagcc cgagggtcag gtacgggctc
    17941 gtcgaatcgg ccagctggaa gaccggggag cggctccgga tcgagtagtg cagcgcgtcc
    18001 tccccgggca gggaccgctg cgcgtgcgct ccggccggcg ccaggagcac cgccgccagg
    18061 aggagcgcgc cgtttcggat gcagccggtc atggatcttg gttcgttcgg atacacgcca
    18121 ggtaggcctc gtgcacgcct tccgccaccc gccccgtggt gacgacgtcg acgacactcc
    18181 ttacttcctc ggacgcattc gccggggcaa aagaccggcc caccgcccga agcccaacga
    18241 tgtcaccgtc cgtgtcgccg atgaacgccg tctccccgag cgggatccgc agctcgtcgg
    18301 ccagccaggc gaggccctgg gctttcgtga gtcgccggct caggatgtct atcgagaccg
    18361 gagtcgacgc gaccgtgagg tcgggatgcc gctcggcgac ccgggcccgt acgaggggca
    18421 gcatgccgcg gacgtcgtcc cgggccgggc cgatgacgct cgcctgcgtg cgcttgccga
    18481 tgtcgacgaa gagccccgtt ccgcggacgg cggaggccag ccacgagcgc agttcatcga
    18541 gttcccgctc gagggcctcg tcgaagtccg gatgccaggt cgtcctggcc ttcacgggct
    18601 cgaagaggcc cgccccggct tcgaaaagca cggggagggt gagtccgagg gcctgggtca
    18661 tcgcttccgt gtacgcatac ggacgcccgg tgcagaggga gaacgccggc ggggctcccg
    18721 gtacgcccca ggggtcgccg cgggccgcca ggcgtgctat ggcgtccagt cgcttgcggt
    18781 cgaagggtcg gaagggctcg gacaggcagc cgtcgatgtc cgagacgaac agtcggatca
    18841 ttcgactccg aagacgtcct tgagcgcgtc gaccaggccg tcggaaa
//
LOCUS       DBNHQZ010000084         4483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_972978, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000084 DBNHQZ010000000
VERSION     DBNHQZ010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_972978"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..635)
                     /locus_tag="AC16YU_02170"
     CDS             complement(66..635)
                     /locus_tag="AC16YU_02170"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6064053.1"
                     /translation="MSTQTRVKLNGLDTTQMFETVEAIKSDSNVALFEFRAKNRWIDG
                     GANESTIQGFYGALQEDTSRPEPFVLMNGEPPVLLGDNEGANPVEFLLHALAGCVTTT
                     FVIHATARGIEIESISTSLDGDVDVRGILALDDSVPAGYREIRMKMDIKANASDQEID
                     ELLAFVADHSPVCNTIRRPVPVKVKRVAA"
     gene            complement(860..2770)
                     /locus_tag="AC16YU_02175"
     CDS             complement(860..2770)
                     /locus_tag="AC16YU_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6064054.1"
                     /translation="MPLSAAVDPAVLRAIVEGTATETGDGFFDALCRHMAEAMRVKAT
                     WVTEWYPEERRLRALSFWDGDRFVTGFEYCIEGTPCEPVIENRTLVHVPDRIVELYAA
                     DPSLEPLGAVSYLGVPLFDTDDEILGHLAVLHDAPMDADESLLAVFRIFAGRAAAELR
                     RKRRDHALRDRELRFSRLLEGAMDAIIECDGDRRITTANAAAARVFGASLEDLPGRPF
                     DAFLLPESRGKLSYLIAQLERQPEGRKSLWIPNGLQCVTVSKEKFPADATVSCYGEAG
                     ARYYTVVLRNVQAQAEAQERIRSLSGEADYLRSELASIQGFDEIIGESAALRALLADV
                     ERVAGTDAAVLVTGETGTGKELIARAIHRRSPRASGPLIAVNCAAIPENLQESEFFGH
                     EKGAFTGALAQRDGRFLLADGGTLFLDEVGEMGLDLQAKLLRVLQEGVIEPVGGSRTI
                     PVDVRVVAATNRDLARMVEEGAFRADLMYRLNVFPLHLPPLRERGGDVILIAEAIVQE
                     IARRRGTVAAPLTDSMKERLLRYDWPGNVRELRNVVERAFITSTDGRTLNLVRALPDA
                     GPPAASDGPERGEAILTEREMKDFERRNIERALTAAGGRISGAGGAAELLGVNPNTLT
                     SRMKTLGVERPR"
     gene            2934..>4483
                     /locus_tag="AC16YU_02180"
     CDS             2934..>4483
                     /locus_tag="AC16YU_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR6064055.1"
                     /translation="MKALVLTVLAFAWTLILAEPTLGQVASFGRISATAGGFAGVLAD
                     GDGFGSDVSPLGDLDGNGVPDLAVGAPGDDYGGTDLGAAWILFLDHTGSVTGHGKIRQ
                     NSPGFTGGRLGDGDRFGERLVRVGDLNGDDIPDLTVAAPSDDDGGADRGAVYTLFLTA
                     GGQAQATAKISHAFGGLSGGLRDGDRFGAGLDALGDVNGDGLGDLVVGAPGDDDGGPD
                     RGAVWILRMNSNATIKSQQKVSSTQGGFLGALADGDRFGSGVADTGDVDGDGIDDLAV
                     GAAGSADGGAVWILFLNADGTVRAWQKLAHGEGGVDFAYQSIGTGVAGPGDLDGDGVP
                     DVALADPGMDSGRGGLWIVFLNPDGTARGSAAADGSALGGQLADGDAFPSSVRALGDV
                     DGDGAVDFGAGHPTRDDGGPDRGAAWILFGSYEDVSAQKLAAPANGSSDLATNLTLQW
                     EALVGTMSYTLQVATSADFAGPFVNAQGLIGTSRAVTGLSPLTTYFWRVQPASLQGLG
                     GWSATWSFT"
BASE COUNT          617 a         1602 c         1577 g          687 t
ORIGIN      
        1 gtagtcggcc gcttcgcccg gccttccgcc gggcccgccg accgtcgtcg ggcgtcccga
       61 gggagtcagg ccgccacgcg cttcaccttc accggcaccg gcctgcggat cgtgttgcag
      121 acgggcgagt gatcggccac gaaagcgagc agctcgtcga tctcctggtc cgaagcgttc
      181 gccttgatgt ccatcttcat gcggatctcc cggtagccgg cgggcacgga gtcgtccagg
      241 gccaggatgc cccgcacgtc gacatcgccg tccaggctcg tggagatcga ctcgatctcg
      301 atgccccgcg ccgtcgcgtg gatcacgaag gtcgtcgtga cgcagccggc gagggcgtgc
      361 agcaggaact cgaccgggtt cgcgccctcg ttgtcgccga gaaggaccgg cggctcgccg
      421 ttcatgagga cgaagggctc cgggcgcgag gtgtcctcct gcagggcgcc gtagaagccc
      481 tggatcgtgg actcgttggc gccgccgtcg atccagcggt tcttcgcgcg gaactcgaag
      541 agggcgacgt tggaatccga cttgatggcc tccaccgtct cgaacatctg ggtggtgtcg
      601 aggccgttca gcttgacgcg ggtctgggtg ctcatttccg tgtacctgtg tgaggttggt
      661 tgggttgtcg atcgtcggcc cgagcgttgc gtgccgttga ccagtgcctt tcccataccg
      721 tgccaagtcc ggggcgtcat caggtgactg atgtcccaca acgagttacg gtctcctcga
      781 tcgatcccac tcgatcacga catttcgtga ttcacgacat ctcgtgaccg cgctcacgaa
      841 tcctcgcggg tcaccgggct cagcggggcc gttccacgcc cagcgtcttc atgcgggagg
      901 tgagcgtgtt cggattcacc ccgaggagtt ccgcggcgcc cccggcgccc gagatccggc
      961 ccccggccgc ggtgagcgcc cgctcgatgt tccggcgctc gaagtccttc atctcgcgct
     1021 cggtcaggat cgcctcgccc ctctccggtc catccgacgc ggccggggga ccggcatccg
     1081 ggagcgcccg gacgaggttc agcgtccggc cgtcggtgga cgtgatgaag gcgcgctcga
     1141 cgacgttgcg cagctcgcga acgttgcccg gccagtcgta gcgcaggagc cgctccttca
     1201 tggagtcggt gagcggagcc gccaccgttc cgcgccgccg cgcaatctcc tgcacgatgg
     1261 cctcggcgat caggatcacg tccccgcccc gctccctgag cgggggcagg tggagtggaa
     1321 acacgttcag gcggtacatg aggtccgccc ggaaggcccc ctcctccacc atgcgcgcca
     1381 gatcgcggtt cgtggcagcg accacacgga cgtccacggg gatggtccgc gagccgccca
     1441 ccggctcgat aacgccctcc tgcagcacgc gcaggagctt ggcctgcagg tccaggccca
     1501 tctccccgac ctcgtccagg aacagggtcc caccgtcggc cagcaggaat cggccgtccc
     1561 gctgcgcgag cgccccggtg aacgcgccct tctcgtgccc gaagaactcg ctctcctgca
     1621 ggttctctgg gatcgcggcg cagttcaccg cgatgagcgg gcccgatgcg cgggggctgc
     1681 gcctgtggat cgcccgggcg atcagctcct tccccgtccc cgtctctccg gtcacgagca
     1741 cggcggcatc ggtcccggcc acgcgctcga cgtcggcgag cagggcgcga agggccgcgg
     1801 actcgccgat gatctcgtcg aatccctgga tcgaggccag ctcgctgcgc aggtagtccg
     1861 cctcgcccga gagcgagcgg attcgctcct gggcctcggc ctgcgcctgc acgttgcgca
     1921 gcaccaccgt gtaatacctc gcgcccgcct ccccgtagca cgaaacggtc gcgtcggcgg
     1981 gaaacttctc cttcgagacg gtgacgcact gcaggccgtt cggtatccag agcgacttcc
     2041 ggccctcggg ctgccgctcg agctgcgcga tcaggtacga cagcttgccc cgggattcgg
     2101 ggagcaggaa cgcgtcgaac ggccggcccg gcaggtcttc cagcgaggcc ccgaagacgc
     2161 gggccgcggc ggcgttcgcg gtggtgatcc tgcgatcgcc gtcgcattcg atgatggcgt
     2221 ccatcgcgcc ctcgagaagc cgcgagaacc gcagctcgcg atcgcggagg gcatggtccc
     2281 gccgcttccg gcgaagctcg gccgccgcgc ggccggcaaa gatgcggaag acggcgagga
     2341 gcgactcgtc cgcgtccatc ggcgcgtcgt ggagcacggc gaggtgaccg aggatctcgt
     2401 cgtccgtgtc gaagaggggc acgccgaggt agctcacggc gccgagcggc tcgaggctcg
     2461 ggtcggccgc gtacagctcc acgatccggt ccggcacgtg gacgagcgtc ctgttctcga
     2521 tcaccggctc gcacggcgtc ccctcgatgc agtactcgaa gcccgtcacg aaccgatcgc
     2581 cgtcccagaa cgagagcgcg cgcagccggc gctcctccgg gtaccactcg gtgacccagg
     2641 tggctttcac gcgcatggcc tcggccatgt ggcgacacag cgcgtcgaag aagccgtcgc
     2701 ccgtctccgt ggccgtgccc tcgacgatgg cgcggaggac tgcggggtcg acggcggcgg
     2761 acaggggcat cgcgttccga gagccgccct cggacggcct gctgtggggg cgacgcggaa
     2821 agtagggcac gcgcggagaa ttatcggacg gccggtttcg tccgatccct gactggtcga
     2881 aacccagtcg gcgtggccgc cctgccgggt cgccactccg gaggagtcac tgcatgaagg
     2941 ccctcgtact gacggtcctg gcctttgcct ggaccctcat cctggccgag cccacgctcg
     3001 gccaggtggc gtcgttcggc cggatcagcg ctacggcggg cggcttcgca ggcgtgctgg
     3061 ccgacggcga cggcttcggc tcggacgtct cgcccctcgg cgatctggac gggaacggcg
     3121 tcccggacct cgcggtcggc gcgcccggcg acgactacgg cgggaccgac ctcggtgccg
     3181 cgtggatcct gttcctggac cacacgggct cggtcacggg ccacggcaag atccggcaga
     3241 acagtcccgg cttcaccggc ggtcggctgg gcgacgggga ccgcttcggc gagcgcctgg
     3301 tccgcgtcgg tgacctgaac ggcgacgaca ttcccgacct gaccgtggcc gcaccctccg
     3361 acgacgacgg cggggccgac cggggcgccg tctacaccct cttcctgaca gccggcggcc
     3421 aggcgcaggc cacggcgaag atcagccacg cgttcggcgg gctctcgggc gggctgcgcg
     3481 acggggaccg gttcggggcc ggcctggacg ccctcggcga cgtgaacggc gacggcctgg
     3541 gcgacctcgt ggtgggcgcg cccggcgacg acgacggtgg cccggaccgc ggggccgtgt
     3601 ggatcctgcg catgaactcg aacgccacga tcaagtccca gcagaaggtg tcctccaccc
     3661 agggcggctt tctcggggcg ctcgccgacg gggatcgctt cggctccggc gtggccgaca
     3721 ccggcgacgt ggacggggac gggatcgacg acctcgccgt cggcgcggcg ggcagcgcgg
     3781 acggcggcgc cgtctggatc ctgttcctga atgccgacgg caccgtgcgc gcctggcaga
     3841 agctcgccca cggcgagggc ggagtggact tcgcgtacca gtcgatcggc accggcgtcg
     3901 ccgggcccgg cgacctggac ggggacggcg tgcccgacgt ggcgctcgcc gatccgggca
     3961 tggactcggg ccgcgggggc ctctggatcg tgttcctgaa ccccgacggc accgcgcgcg
     4021 gctcggccgc ggctgacggc tcggcgctgg gcggccagtt ggcagacggt gacgcgttcc
     4081 cttcgtccgt gcgggctctg ggcgacgtgg acggagacgg cgcggtggac ttcggcgccg
     4141 gccacccgac ccgcgacgac ggcggacccg accgaggcgc ggcgtggatc ctcttcggct
     4201 cgtacgagga cgtctcggcc cagaagctcg ccgcgccggc caacggctcc tccgacctcg
     4261 ccaccaacct cacccttcag tgggaggccc tcgtgggcac catgtcgtac acgctgcagg
     4321 tggcgacctc cgcggacttc gcaggaccct tcgtgaacgc ccaggggctc atcggcacgt
     4381 cccgcgccgt gaccggcctc tccccgctca ccacgtattt ctggcgcgtc cagcccgcca
     4441 gcctccaggg gcttggcggc tggtccgcca cctggagctt cac
//
LOCUS       DBNHQZ010000085         4356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2056207, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000085 DBNHQZ010000000
VERSION     DBNHQZ010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2056207"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2193
                     /locus_tag="AC16YU_02185"
     CDS             <1..2193
                     /locus_tag="AC16YU_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6064056.1"
                     /translation="AKTNQSTMLTRMGSTLGTVAYMSPEQARGHEVDGRTDLYSLGTM
                     LYEMVAGKLPFGGDYEQAVLYGILNEPPEPLTALRTGVPMELERIVAKLLSKEAEYRY
                     QTAADLLADLKSLSLGASGVSRRSIPAMSGAPVAATPAPAGGLPKWAYGAIAGALVLG
                     AAGAWLAKPAPSDRDIPLMRTRLVLEDAVSPLSAAITPDGTRLFYVAQANEGDQAMLA
                     VYDFRTGISTRIPGTAGAQLPVLSPDGRWVAFRVEAQWWRMPAEGGEPSLLPGTASQS
                     RNAGFAIDGSLHFTAPGWRSVFRYDDAEGLSTVVTDTSSFISGIVPLGSRGRMIYGGW
                     SGPSNPKGSYLYGGDLDEPRLILDGANVHGVTENGYALFVRGGEAPQTLAIPIDLETG
                     EARGSEVALGRFSVPLAVSRTGVLVYEERPAETGGVIPADLYRVDSEGRAEKLFRMPG
                     AGSQEIAVSRDGSRLLVSARASQESLPDMYVVDMETGTPTRLTQGGYYDVPSWSHDDR
                     LIYFDHQPPGDTAWRLMRRRADGLGSNELVLPDSLDVGDPSLSANGRYLVFVDANDIR
                     LIDLHADTTAWLVREPGAQVKPRVSPDSRYLVYVDSGDAADYQCGSIQVRPLLQQGSA
                     IELATRGCMPAWGRDGSAVYYEQDGSIWRVPVTTAPAFRNLGLPEVAYAGPAGRAPNS
                     DRGVFFDTAPDGTLFVSYPEGSRSGGDRPIWVVTRWFEELERLAPRFD"
     gene            2207..>4356
                     /locus_tag="AC16YU_02190"
     CDS             2207..>4356
                     /locus_tag="AC16YU_02190"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6064057.1"
                     /translation="MTGLHLAQYRIDAELGRGGMGIVYRATDTKLNRAVALKVLPAAA
                     LSSGDDRARFMREAQSAAQLHHPNIATVFGIDEAVPVQPGVAPQPGTEARLFIAMELI
                     EGETLDARIRRGPMKLEEVVRIGIQIAEALKEAHAKQIVHRDIKSANVMLTQAGVAKV
                     LDFGLAKTNQSTMLTRMGSTLGTVAYMSPEQARGQEVDGRSDLYSLGTVLYEMIAGRL
                     PFGGDYEQAVLYGIMNEDPEPLTAVRTGVSMDLERIVFKLLAKEQRHRYQTASDLVVD
                     LETAGLQPSGSTTRTTIRAPRPSSWSARRTASTAGIAFAAGAVLVALAWALWPSPAPA
                     PRPQQVLDVKLAADHPIVTADLSADGSTLVYATNDLGRDNVFVRDLATGEVRRIAGSA
                     GALVVEMSPDGESVLITKQLTIERTSLRSGTPLLVVEAEEGNPRANWGPPGWVIYEDL
                     QALWKVSLETGEKAPLAIRPVGGMEQDYDWPSLLPDGRTVMAQVENSDGTTGIGFWDF
                     ESGDRLGTTAVGGVRPQYVASGHLVFSAPIVGATGNLVSVPFDAGRLRMLGAPSPIAA
                     EVLSTTVAVSGEGTLVYRRSATGAEVSRSTRPLAGIQVAEFPGINRIVPVTPARFADI
                     ELSPDETRAVLTIQDPSLNTGQGGGTDVFVLVVARGTLLQLTFNATGGSPTWVAGGDS
                     VAYVDLSSSDRGIYGVNVRAADGTGSPRLLFRSVV"
BASE COUNT          655 a         1449 c         1579 g          673 t
ORIGIN      
        1 gcgaagacga accagtcgac gatgctgacg cggatgggct cgacgctcgg gacggtcgcg
       61 tacatgtcgc cggagcaggc ccgcggccac gaggtcgatg gaaggacgga cctgtactcg
      121 ctcggcacga tgctctacga gatggtcgcc gggaagctgc ctttcggggg cgactacgag
      181 caggcggtgc tctacgggat cctgaacgag ccgccggagc ccttgacggc gctgcggacg
      241 ggggtgccga tggagctgga gcggatcgtc gcgaagctgc tctccaagga ggcggagtac
      301 cgttaccaga cggcggcgga tctgcttgcg gacctgaaga gtctgagcct gggcgcgagc
      361 ggggtgtcga ggcgaagcat cccggcgatg agcggggcac ccgtggcggc gacgcccgcg
      421 ccggccggcg gactcccgaa gtgggcctac ggagcgatcg ccggtgccct ggtgctcggg
      481 gccgccgggg cgtggctcgc gaagcccgcg ccgtccgacc gcgacatccc gctcatgcgg
      541 acgcgcctgg tcctggaaga cgccgtctcc cccctttccg ccgcgatcac gcccgacggc
      601 acgcgcctgt tctatgtcgc gcaggcgaac gaaggcgatc aggcgatgct ggccgtttac
      661 gatttccgca ccggtatctc cacccggatt ccgggcacgg cgggtgccca gctgcccgtc
      721 ctgtcgccgg acggccgctg ggtcgcgttc cgcgtcgagg cgcagtggtg gcggatgccc
      781 gccgagggag gtgagccttc gcttcttccc ggaacggctt cacagtcgcg caatgccgga
      841 ttcgcgatcg acggcagcct gcatttcacg gcgcccgggt ggcggagcgt gttccgctac
      901 gacgacgcgg aagggctgtc gaccgtggtg acggacacgt ccagtttcat ctcgggaatc
      961 gtccccctgg ggagcagagg caggatgatc tacgggggat ggtccggacc ctcgaatccg
     1021 aaagggtcct acctgtacgg tggggatctc gacgagccgc gcctcatcct ggacggcgcc
     1081 aatgttcacg gcgtgaccga gaacggttac gcgctgttcg ttcgcggcgg cgaggccccg
     1141 cagaccctgg ccatccccat cgatctcgag accggagagg cccgcgggtc ggaggtcgct
     1201 ctggggcggt tctccgtgcc gctggccgtg tcgcgaacgg gagtcctcgt gtacgaggag
     1261 cgtccggcgg agacgggagg ggtgattccg gccgacctgt accgggtgga ttccgagggc
     1321 cgggcggaga agctgttccg gatgcccggc gcgggatcac aggagatcgc cgtttcacgc
     1381 gacggctcga ggctgctcgt gagcgcccgt gcaagccagg aatccctgcc cgacatgtac
     1441 gtcgtcgaca tggagaccgg gacgcccacc cgcctcaccc agggcgggta ctacgacgtc
     1501 ccgtcgtggt ctcacgacga ccggctcatc tatttcgacc accagccacc cggggacacc
     1561 gcatggcggc tcatgcgtcg tcgcgcggac ggcctggggt cgaacgagct cgtgctcccg
     1621 gacagtctgg acgtgggcga tccgtccctg tcggcaaacg gccgctacct cgtcttcgtc
     1681 gacgccaacg acatccggtt gatcgacctg cacgccgaca ccacggcctg gctcgtccgg
     1741 gagcccggag cccaggtgaa gccgcgggta tcgcccgaca gccgctacct ggtctacgtc
     1801 gacagcggcg acgcggccga ttaccagtgc ggatcgatcc aggtgcgacc cctcttgcag
     1861 cagggcagcg cgatcgagct ggcgacgagg ggctgcatgc cggcctgggg cagggacggg
     1921 agcgcggtct actacgagca ggacgggtcg atatggaggg ttcccgtcac cacggcgcct
     1981 gcgttccgaa atctcgggct gccggaggtc gcgtacgccg gaccggccgg ccgggctccc
     2041 aacagcgatc ggggggtgtt cttcgacacc gctccggacg gcacgctctt cgtctcctac
     2101 ccggaaggat cacgttcggg aggcgaccgg cccatctggg tcgtcaccag gtggttcgag
     2161 gagctcgagc ggctcgcccc ccggttcgat tgacctccct gccaccatga ccggcctcca
     2221 cctcgcccag taccgtatcg acgccgagct cggccgcggc ggcatgggca tcgtgtaccg
     2281 cgcgacggac acgaagctca atcgggcggt tgcgctgaag gtgctgccgg ccgccgcact
     2341 gtcctcgggc gacgaccgcg cccgcttcat gcgcgaggcc cagtcggcgg cgcagctcca
     2401 tcacccgaac atcgccactg tcttcgggat cgacgaggcc gtacccgtgc agcccggtgt
     2461 cgcgccccag cccggcaccg aggcgcggct cttcatcgcg atggagctca tcgaggggga
     2521 gacgctggac gcgcgcatcc gccgcggtcc gatgaagctc gaggaggtcg tgcgcatcgg
     2581 catccagatc gcggaagcgc tgaaggaggc gcacgcgaag cagatcgtgc accgggacat
     2641 caagtccgcg aacgtgatgc tcacgcaggc gggcgtcgcg aaggttctcg acttcgggct
     2701 cgccaagacg aaccagtcga cgatgttgac acggatgggc tcgaccctcg gcacggtggc
     2761 gtacatgagc ccggagcagg cgcggggcca ggaggtggac gggcggagcg acctgtactc
     2821 gctcgggacc gtgctctacg agatgatcgc cggacggctg ccgttcgggg gggactacga
     2881 gcaggcggtg ctctacggga tcatgaacga ggaccccgag ccgctgaccg cggtgcggac
     2941 cggggtgtcg atggacctcg agcgcatcgt cttcaagctg ctcgccaagg aacagcgcca
     3001 ccgttaccag acggcctcgg acctggtcgt ggacctggag actgcggggc ttcagccctc
     3061 cgggtccacg acgcggacca cgatccgggc gccgaggccg tcatcgtggt ccgcgcggcg
     3121 caccgcgtcg accgctggga tcgcctttgc ggccggagcg gtgctggtgg ccctcgcgtg
     3181 ggcgctctgg ccttcgcccg cgccggcgcc gcgtccccag caggtgcttg acgtgaagct
     3241 ggcggccgac catcccatcg tgacggccga cctctcggcg gacgggtcga ccctcgtata
     3301 tgccacgaac gacctgggcc gcgacaacgt gttcgtccgt gacctggcca ccggcgaggt
     3361 gcggcgcatc gcgggatcgg ccggagcgct cgtcgtggag atgtccccgg acggcgagag
     3421 cgtgctcatc accaagcagc ttacgatcga gaggacgtcg cttcggtcgg gcacgccgct
     3481 tctcgtggtc gaggcggagg aggggaatcc gagagcgaac tggggtcctc cgggctgggt
     3541 gatctacgag gacctccagg ccctgtggaa ggtgtcgctc gagaccgggg agaaggcgcc
     3601 cctggccatt cgccccgtcg gcggcatgga gcaggactac gactggccgt cgcttcttcc
     3661 ggacggacgt accgtcatgg cccaggtcga gaattcggac gggaccacgg gcatcgggtt
     3721 ctgggacttc gagtcgggcg accgcctcgg gacgacggcc gtcgggggcg tccggcccca
     3781 gtacgtggcg tccggccacc tggtcttcag cgctccgatc gtgggcgcga cggggaacct
     3841 ggtgtccgtt cccttcgacg ccggccggct gcgcatgctc ggggcgccgt cgccgatcgc
     3901 ggcggaagtg ctctctacca cggtggccgt ttcgggcgag ggcaccctgg tgtacaggcg
     3961 gagcgcgaca ggtgcggagg tgagtcgatc cacccgcccg ctggcaggaa tccaggttgc
     4021 cgagtttccg ggcatcaacc ggatcgtgcc ggtcacgccg gcccgtttcg ccgacatcga
     4081 gctgagcccc gacgagaccc gggccgtgtt gaccatccag gatccgtcgc tgaacaccgg
     4141 gcaggggggc ggcacggacg tcttcgtgct cgtcgtggcc aggggaacgc tgctccagct
     4201 cacgttcaac gcgaccggtg gctcgccgac ctgggtcgcg ggaggggatt ccgtcgccta
     4261 cgtggacctg tcgagctccg atcgggggat ctacggcgtg aacgtccggg ccgcggatgg
     4321 caccgggtct cctcgcctcc tgttccggag cgtggt
//
LOCUS       DBNHQZ010000086         5375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2191471, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000086 DBNHQZ010000000
VERSION     DBNHQZ010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2191471"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..581)
                     /locus_tag="AC16YU_02195"
     CDS             complement(87..581)
                     /locus_tag="AC16YU_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064058.1"
                     /translation="MLRTNCRLTALAVALLVAGTATADPPGLVLQTDEAALMTPTEWV
                     AYSERISEALASGQDGLREAALRMTILYADRLSLDHGDTVEMVRVFRDHRNDRMRRLA
                     VVALHAADDHWGIDMLARSYRFEDCKAVRYQIGAVLLDHAMRHREGVIELEHPKAIVV
                     WPVE"
     gene            853..1614
                     /locus_tag="AC16YU_02200"
     CDS             853..1614
                     /locus_tag="AC16YU_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HIR6064059.1"
                     /translation="MASYFAFDYAGGAFELFGPAHLVTLAVIAALTAWLARSGRAAKG
                     DGRRAMRRALAVVLLANEASWHAWNLAHGAWDPGHMLPLHACSVMVWIAVAVLWTGYR
                     PLYAPVYFLGVAGALQALITPDAGIYGFPHFRYLQTMIAHGGLVAAGIWVVTAERYAP
                     SARDGVRVLVSLNVYALAVYFVNGWIGSNYLYVNAKPDTASILDAMPGWPWYLPILEL
                     LAVAFFALLLLPFRRRPVAAAPAESVPALERVGHA"
     gene            1761..2171
                     /locus_tag="AC16YU_02205"
     CDS             1761..2171
                     /locus_tag="AC16YU_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064060.1"
                     /translation="MNRFFRLATLAAVALLLAACQSEIVDNTDEGDPVVDVSYSAQVQ
                     PIFSSSCSGVGCHIGETTNGVNLSSYAQVMASAGVQYGQAIVVAGDADASPIIDKLSA
                     NPDFGVRMPRERSPLTASQVQIIRTWIDEGALDN"
     gene            2176..2724
                     /locus_tag="AC16YU_02210"
     CDS             2176..2724
                     /locus_tag="AC16YU_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR6064061.1"
                     /translation="MPRLLSVLALAALTIPAPSSAQAFRTDSGTAEFTSSVPLHTFTG
                     TSDRLEGRIDLADGTVDFFLDLETLETGIGKRDKDMRKTLETDEYPFASFYGRFVSPV
                     DSTSTVEQPVRVTGAFSIHGVERGIEVDGTVQRTAEGLRVRAEWTLNLKDYDIAPPRL
                     LIVKVDEVQRLRIDALLTPETP"
     gene            2721..3602
                     /locus_tag="AC16YU_02215"
     CDS             2721..3602
                     /locus_tag="AC16YU_02215"
                     /inference="COORDINATES: protein motif:HMM:NF040196.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HIR6064062.1"
                     /translation="MTPSLTRTGLVAILVTLLAVQAVSAQIERRRADPSGPVDELFWA
                     PSLVTTASVTNVPRGNLNVTIMHAFGLVSDGLDEWFGMDAGANIRIGLDYGLTDRISI
                     GIGRSRLEKVVDLRTKVSLLRQSRDGRMPVEVAVKGDLGIRTEPGGFDFADRLSYLGA
                     VLVARRMSDRVSLQLTPMVSHFNTVFAEVGPGGAPRIEENTHVAVGLGARLVLTDRLA
                     VLAEYIPVIGERSDGTRDAMALGLDIETGGHVFQVFVTSSQWLTEQHVVARNVDDFLA
                     GDFRIGFNVNRVFGPGG"
     gene            complement(3609..>5375)
                     /locus_tag="AC16YU_02220"
     CDS             complement(3609..>5375)
                     /locus_tag="AC16YU_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064063.1"
                     /translation="TNLVKGYDPATRSFATTWSDIDVSCEACHGPGSRHVAWARRKAR
                     FPLLYPDGDSLTEAAIGLVVSIHDREALAWRMDPATGTARRTRPRGTAQDLPTCARCH
                     ARRASLLPEYRFGLPFEDTHVLEPPVPPLYHADGQVLEEDYVYGSFLQSRMYRAGVSC
                     GNCHDAHSLRTRAPGNLLCRTCHDPVRFDTTSHHGHVPDTPGSQCVDCHMPETVYMGV
                     DARRDHGFTIPRPALSREAGVPNACTGCHSERTDEWAIRALARLGQAPASPPFALAFH
                     AARTGAPDAEDRLLSVAADTAEANVRRAGALRLLRSAAGRRTVPFVETILSSRQPALR
                     WAAIGLLELFPPPERARLGAPLLDDPVRSVRIEAARVIADPAARSGLKPADGPAFDRA
                     LAEYMDVLRMDADRPEAQANLGLVLAALGDSAGAENAFREAIRLSPWFTQAIVNLADL
                     YRAADRDTEGEALLRGGLERQPASADLHHALGLLLVRARRTDEALSELGEAARLRPDE
                     IRFGYVHAVALHTSGRGREALERIDALLERRPADAELLYLLATVRRDRGETGLALEAA
                     RRLARTHPDDPRGPALVDRLTR"
BASE COUNT          745 a         1876 c         1934 g          820 t
ORIGIN      
        1 ggggcgcgcc ccccgcgggg cgcgccccga cgcgtcgggc ggtcgtgccg cccggccgcc
       61 cgtggaagac gtcgtttcgg tgccgtctac tcgacgggcc agaccacgat ggccttcgga
      121 tgctcgagct cgatcacgcc ttcgcggtgg cgcatggcgt ggtccagcag cacggccccg
      181 atctggtagc ggaccgcctt gcagtcctcg aaccggtagg accgggccag catgtcgatg
      241 ccccagtggt cgtcggcagc gtgcagggcc acgacggcca ggcgccgcat gcgatcgttc
      301 cggtggtcac ggaatacccg gaccatttcg acggtgtccc cgtggtcgag gctcaggcga
      361 tccgcgtaca ggatcgtcat cctcagggcg gcctcgcgga ggccgtcctg cccggacgcc
      421 agcgcttccg aaatccgctc cgagtaggcg acccattccg tcggggtcat cagggccgcc
      481 tcgtccgtct ggagcacgag gcccggcggg tccgcggtgg ccgtgccggc cacgagcagt
      541 gcgacggcca gggccgtcag gcgacagttg gtgcgcaaca tggcagtacc ctccctctgg
      601 tacgacagtg attgcgtctg tccgataggt gggagggcgt ttcccgcttc gcccgccggg
      661 cgcctccgtc gccacggatt ctgtcccgga attccccctc ccttgctggt caaggaccga
      721 gggccgttcg ctcgacccac gatctgaaga tgtggtacgg aaggttcact ggagagttgt
      781 acccggcgcc ccgatattcg ggtaggcaat tccccgattc tgcacggagc acctggcccg
      841 gccggacgag cgatggcgtc ctacttcgca ttcgactacg cgggcggcgc gttcgagctg
      901 ttcggtcccg cgcatctcgt cacccttgcg gtcatcgccg ccctgacggc ctggctcgcg
      961 cggtccgggc gcgccgcaaa gggcgacggg cggcgggcga tgcggcgggc gctcgccgtg
     1021 gtgctcctgg cgaacgaggc gtcgtggcac gcttggaacc tggcgcacgg cgcctgggac
     1081 cccgggcaca tgcttcccct gcatgcctgc agcgtcatgg tgtggatcgc ggtggccgtg
     1141 ctctggaccg gctaccggcc gctctacgcg ccggtgtact tcctgggggt ggcgggcgcg
     1201 ctgcaggcgc tcatcacccc ggacgccggg atctacggct tcccccactt ccgctacctg
     1261 cagacgatga tcgcccacgg cgggctggtc gcggccggaa tctgggtagt cacggccgag
     1321 cgctacgcac cgtcggcccg agacggcgtg cgggtgctcg tgtcgctgaa cgtatacgcc
     1381 ctcgccgtct acttcgtgaa cggctggatc ggctcgaact acctgtacgt taacgcgaag
     1441 ccggacacgg cgtccatcct cgacgccatg ccggggtggc cgtggtacct gccgatcctg
     1501 gagctcctgg ccgtcgcgtt cttcgcgctc ctgctcctgc ctttccgccg ccgtccggtg
     1561 gccgccgccc cggcggagag cgtgccggcg ctcgaacgag tgggccacgc ctgatcggcg
     1621 tccccacgaa agtcgttgca acgggtggat gtcgggcgtc gaccgttcac ccgcccgaat
     1681 cgacgggcgg cggcccggat cgcacgcgcg ccgccccgag tcgctaccgt gaccggcgct
     1741 acccgtccga cgtgataccc atgaaccgct tcttccgcct tgcgacgctc gccgccgtcg
     1801 cacttctcct ggctgcctgc cagagcgaaa tcgtggacaa taccgacgag ggagaccccg
     1861 tggtggacgt ctcgtactcg gcccaggtgc agcccatctt cagctcttcg tgctcgggcg
     1921 tcggctgtca catcggcgaa accacgaacg gcgtgaatct gtcgagctac gcccaggtga
     1981 tggccagcgc gggcgtgcag tacgggcagg cgatcgtcgt ggccggggat gcggatgcga
     2041 gtccgatcat cgacaagctg tccgcgaacc cggacttcgg cgtgcgcatg ccccgggagc
     2101 gctcgccgct cacggcgtcc caggtccaga tcatccgcac ctggatcgac gagggcgccc
     2161 tcgacaactg atcccatgcc gcgcctcctg agtgtcctgg ccctcgcggc cttgacgata
     2221 ccggcaccct cgtccgcaca ggcgttccgc acggactcgg gcaccgcgga gttcacctcg
     2281 tcggttccac tgcacacgtt cacgggcacg tcggaccggc tcgagggccg gatcgacctc
     2341 gcggacggca cggtggactt cttcctggac ctcgagaccc tcgagacggg catcggaaag
     2401 cgggacaagg acatgcggaa gacgctcgag accgacgagt acccgttcgc ctccttctac
     2461 ggccggttcg tctcgcccgt cgattccact tcgaccgtcg agcagccggt ccgcgtgacc
     2521 ggcgcattct cgatccacgg ggtcgagcgc gggatcgagg tggacggcac ggtgcagcgc
     2581 actgcggagg gcctgcgggt ccgcgcggag tggacgctga acctcaagga ctacgatatt
     2641 gccccgccgc gactgctgat cgtgaaggtg gacgaggtcc agcggctccg catcgacgcc
     2701 ctgctcacgc ccgagacgcc atgacaccct ccctgacgcg aaccggcctc gtcgccatcc
     2761 tcgtcaccct gctcgccgtc caggcggtct ccgcacagat cgagcggcgc cgggccgatc
     2821 cgtcgggacc ggtcgacgag ctcttctggg ccccttccct cgtgaccacg gcctcggtca
     2881 cgaacgtccc gcggggcaac ctgaacgtca cgatcatgca cgccttcggg ctcgtgtcgg
     2941 acggcctgga cgagtggttc ggcatggacg cgggcgcgaa catacggatc ggtctcgact
     3001 acgggctgac cgaccggatc tcgatcggca tcggacgcag tcggctcgaa aaggtggtgg
     3061 acctgaggac gaaggtgagc ctgcttcgcc agtcgcgtga cggacggatg ccggtggagg
     3121 tggccgtgaa gggcgacctg ggcattcgca ccgagcctgg cggcttcgac ttcgcggacc
     3181 gcctgtcgta cctgggcgcg gtgctcgtgg cccggcgcat gtcggaccgc gtgagcctcc
     3241 agttgacccc gatggtctcc cacttcaaca cggtgttcgc ggaggtcgga ccaggaggcg
     3301 cgccccgcat cgaggagaac acgcacgtgg ccgtgggcct cggggccagg ctcgtgctga
     3361 ccgaccggct ggcggtcctc gccgagtaca ttcccgtgat cggcgagcgc tccgacggca
     3421 cgcgcgacgc gatggctctg gggctcgaca tagagacggg cgggcacgtg ttccaggtgt
     3481 tcgtgacctc gtcgcagtgg ctcaccgagc agcacgtcgt cgcccggaac gtggacgact
     3541 tcctggccgg cgatttccgc atcgggttca acgtgaaccg ggtgttcggg ccgggcggct
     3601 gacgcggctt accgcgtgag ccggtcgacg agggcgggtc cgcgcggatc gtccggatgc
     3661 gtccgggcga gccgcctcgc cgcctcgagg gcgagccccg tctccccgcg gtcgcgccgg
     3721 acggtcgcca ggaggtacag gagctcggcg tcggcggggc gccgttcgag cagggcgtcg
     3781 atccgttcca gggcctcgcg cccgcgcccc gaggtgtgca gggcgaccgc atggacgtag
     3841 ccgaagcgga tctcgtcggg tcgaagccgc gcggcctcgc ccagctcgga gagggcctcg
     3901 tcggtccggc gggcgcgcac cagcagaagt cccagcgcgt ggtgcaggtc ggccgaggcg
     3961 ggctgccgct ccaggccgcc gcggagcagc gcctctccct cggtgtcccg gtccgccgcg
     4021 cggtacaggt cagcgagatt cacgatcgcc tgcgtgaacc agggcgacag gcggatcgcc
     4081 tcccggaagg cgttctcggc tcccgcggag tcgccgagcg cggcgagaac caggcccagg
     4141 ttcgcctgcg cctccggccg gtcggcgtcc atccgcagga cgtccatgta ctcggcgagc
     4201 gcgcggtcga acgcgggccc gtccgcgggc ttcaggccgg agcgggcggc cggatcggcg
     4261 atcacacgcg cggcctcgat ccggacggat cggacgggat catccaggag gggcgcgccg
     4321 aggcgggcgc gctccggcgg cgggaacagc tccaggagac cgatcgccgc ccaccgaagc
     4381 gcgggctggc ggctcgagag gattgtctcg acgaaaggga ccgtccgccg cccggccgcc
     4441 gacctgagga ggcgcagcgc tcccgcccgt cgcacgttgg cttcggcggt gtcggcggcg
     4501 accgacagga gccggtcctc ggcatcgggc gcgccggtac gggcggcatg gaaggcgagg
     4561 gcgaacgggg gcgatgcggg cgcctgtccg agccgcgcca gggcccggat cgcccactcg
     4621 tcggtgcgct ccgagtggca gcccgtgcac gcgttgggca cgccggcctc ccgcgagagc
     4681 gcggggcgcg ggatcgtgaa gccgtggtcc cgccgggcgt cgacgcccat gtagacggtc
     4741 tccggcatgt ggcagtcgac gcactgcgaa ccgggcgtgt cgggcacgtg cccgtgatgc
     4801 gaggtggtgt cgaatcgtac cgggtcgtgg caggtccggc acaggaggtt gcccggcgcg
     4861 cgggtccgca ggctgtgcgc gtcgtgacag ttgccgcacg agacgccagc gcggtacatc
     4921 cggctctgca ggaaggagcc gtacacgtag tcctcctcca gcacctggcc gtcggcatgg
     4981 tagaggggcg ggaccggcgg ctccaggacg tgcgtgtcct cgaacgggag cccgaaccgg
     5041 tactccggaa gcaggctcgc gcggcgcgcg tggcagcggg cgcaggtggg caggtcctgc
     5101 gccgtgcctc ggggccgcgt ccgccgcgcc gtgccggtcg cggggtccat ccgccaggcc
     5161 aacgcctcgc ggtcatggat cgagacgacg aggccgatgg cagcctccgt cagggagtcg
     5221 ccgtccgggt agagcagcgg aaaacgtgcc ttccggcggg cccacgcgac gtggcgggag
     5281 ccgggcccgt gacacgcctc gcacgatacg tcgatgtcgg accacgtcgt cgcgaacgag
     5341 cgggtcgccg ggtcgtaccc tttcacaagg ttcgt
//
LOCUS       DBNHQZ010000087         3023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1650737, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000087 DBNHQZ010000000
VERSION     DBNHQZ010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1650737"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="AC16YU_02225"
     CDS             complement(<1..979)
                     /locus_tag="AC16YU_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HIR6064064.1"
                     /translation="MLHLQHLTEAHPVEPFDRALQLPQPGSEEERRAFSRLQERLVTQ
                     FRDVFPDPRAPRTVVVVPSLSMNPALLEKISGVQYYEERLLFMLMLLRLPNTRVLYVT
                     SQPIAPSIIDYYLNLLPGVPVSHARKRLELFSAYDTSPVPLTRKLLERPRLVRRIRHA
                     IPDRQVAHLTVYNVTDVERTLAVWLDIPLYGCDPDRCPIGTKSGSRRVFREAGIDHPP
                     GFEDVHGADEMAEALAELRGRHPGLAKAVVKLDEGISGEGNATFDYADAPTGSALAPW
                     IRRLLPRRLSFEASAESWEAYSSQFQSMGGVVEAYIDGIGKRSPSMQGGV"
     gene            complement(1069..2085)
                     /locus_tag="AC16YU_02230"
     CDS             complement(1069..2085)
                     /locus_tag="AC16YU_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR6064065.1"
                     /translation="MDLSTTYLGLKLRNPLVPSASPLSHSVTSIRRMEDAGAGAVVMH
                     SLFEEQLTQQSLELDHFLTYGAESFSEALSYFPEAGAYAVGPDTYLEHVRAAKESVGI
                     PVIGSLNGVSSGGWTSFAKQIEQAGADALELNIYYVPTEPHLTGASVEQMYVDVVSDV
                     VGAVSIPVAVKLSPFFSSIPNISLRMAEAGARGLVLFNRFYQPDLDLESLDVVPRLVL
                     STSEELRLPLRWIAVLFGRVPIDFALTSGVHTFEDVLKGLMAGARVTMMASELLANGI
                     GRLREILEALRFWMEEREYESVAQMQGSMSQQHVAEPAAFERANYVRTLQSWSPDPTG
                     RMIH"
     gene            complement(2133..>3023)
                     /locus_tag="AC16YU_02235"
     CDS             complement(2133..>3023)
                     /locus_tag="AC16YU_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6064066.1"
                     /translation="AADQPDEAAIGAQRSRVDRLRDKLQGRTDPESVDLLGLLDTLVR
                     KTVWIVGGDGWAYDIGFGGLDHVLASGRNVNILVMDTEVYSNTGGQASKATGLGAVAK
                     FAAGGKPTPKKDLGLLAMSYGYVYVAQIAMGADENQTIKAIREAEAYDGPSLVIAYSH
                     CIAHGIDMSKGLDQQRRAVESGHWPIYRFDPERKKTDAVPFQLDCKTPSIPLSEYFYN
                     ESRYRMLRQTDAEAAAAFLKQAQLNVEEKRRQYERMAAPPVASGDGAPGGPPAPGARK
                     PTLPPGRPAPAPGPKRPPQG"
BASE COUNT          459 a         1013 c         1068 g          483 t
ORIGIN      
        1 cgacgccccc ctgcatggag ggcgagcgct tcccgatgcc gtcgatgtac gcctcgacga
       61 ctccgcccat cgactggaac tggctcgaat aggcttccca cgattccgcg gaagcctcga
      121 agctcagccg gcgaggcagc agccggcgga tccagggcgc gagggcgctc ccggtcggag
      181 cgtcggcgta gtcgaaggtg gcattcccct cgcccgagat gccctcgtcc agcttgacga
      241 ccgctttcgc gaggcccgga tgccggccgc ggagctcggc cagggcctcg gccatctcgt
      301 ccgctccgtg cacgtcttcg aagcccgggg gatgatctat gcccgcctcc cggaacacgc
      361 ggcggcttcc gctcttcgtc ccgatcggac accggtccgg gtcgcatccg tagagcggga
      421 tgtcgagcca cacggccagc gtgcgctcga cgtcggtcac gttgtacacc gtcaggtggg
      481 cgacctgccg atccgggatc gcgtgccgga tcctgcgcac gaggcgcggc cgctccagga
      541 gcttgcgcgt gagggggacc ggcgaggtgt cgtaggccga gaagagctcc aggcgcttcc
      601 gggcgtggct caccggcacg ccgggcagca ggttcaggta gtagtcgatg atcgacggcg
      661 cgatcggctg gctggtcacg taaagcaccc gcgtattcgg caggcggaga agcatgagca
      721 tgaacagcag ccgctcctcg tagtactgga cgcccgaaat cttctccagc agcgccggat
      781 tcatcgacag gctcggaacc acgacgacgg tgcgcggagc cctcgggtcc gggaagacat
      841 cccggaactg cgtcacgagg cgctcctgca gcctggaaaa cgcgcggcgc tcctcctcgg
      901 aaccgggctg aggaagctgg agcgcccgat cgaacggctc gaccggatgc gcctcggtga
      961 ggtgctggag atgcagcatg gggctgcacg atcggtccgt ctactggcaa taaacggccc
     1021 ggcgcccaca agcgccaatc gcggatcttc cccggcaagg ccccgggctc agtggatcat
     1081 ccggccggtc ggatcggggc tccaggactg cagcgtgcgg acgtagttcg ctcgctcgaa
     1141 agcggccggc tcggccacgt gctgctggct catgctgccc tgcatctgcg cgaccgactc
     1201 gtattcgcgt tcctccatcc agaagcgcag cgcctcgaga atctcgcgga ggcgtccgat
     1261 gccgttggcg agcagctccg aggccatcat cgtcacgcgt gcgccggcca tcagcccctt
     1321 gagcacgtcc tcgaacgtgt gcactccgga cgtcagcgcg aaatcgatgg gcacgcgccc
     1381 gaagaggacc gcgatccagc gcagcggcag acgcagctcc tccgacgtgc tgagcaccag
     1441 gcgcggcacg acgtcgagcg attccaggtc caggtcgggc tggtagaacc ggttgaagag
     1501 cacgagcccc cgcgcccccg cctcggccat gcgcagggag atgttcggga tggacgagaa
     1561 gaaggggctc agcttcaccg ccaccggtat cgacacggcg ccgaccacgt cggacacgac
     1621 gtccacgtac atctgctcga cggacgcgcc cgtcaggtgc ggctcggtcg gcacgtagta
     1681 gatgttcagc tccagggcgt cggcccccgc ctgctcgatc tgcttcgcga aggacgtcca
     1741 tccgcccgac gaaacgccgt tcaggctgcc gatgacgggg atgccgaccg attcctttgc
     1801 cgccctcacg tgctcgaggt acgtgtccgg acccacggcg taggccccgg cctcggggaa
     1861 gtacgagagg gcctccgaga aactctcggc gccgtacgtg aggaagtggt cgagctcgag
     1921 gctctgctgc gtcagctgct cctcgaagag cgagtgcatg accacggctc cggcgcccgc
     1981 gtcctccatg cgtcggatgg aggtcaccga gtgcgagagt ggcgaggccg acggaaccag
     2041 cgggttgcgc agcttcaggc cgaggtaggt ggtgctcagg tccatggccg gtcgggggct
     2101 gagggatgtc ggacggtgtc gggagagcag ggtcagccct gcggggggcg cttcggtccg
     2161 ggggccggcg cgggccttcc gggcgggagc gtcggcttgc gggctcccgg tgcgggcggg
     2221 ccgccgggcg cgccgtcgcc cgaggcgacc ggcggcgcgg ccatccgctc gtactgccgt
     2281 ctcttctcct cgacgttcag ctgcgcctgc ttcaggaacg cggcggcggc ctccgcgtcg
     2341 gtctgccgca gcatgcggta ccggctctcg ttgtagaaat actcggacag ggggatggac
     2401 ggcgtcttgc agtcgagctg gaagggcacg gcatcggtct tcttccgctc gggatcgaac
     2461 cggtagatgg gccagtgccc ggactcgacg gcccggcgct gctggtccag gcccttggac
     2521 atgtcgattc cgtgcgcgat gcagtggctg taggcgatga cgagggaggg tccgtcgtag
     2581 gcctccgcct ctcggatggc cttgatcgtc tggttctcgt ccgcgcccat ggcgatctgc
     2641 gccacgtaca cgtaaccgta gctcatcgcg agcaggccca ggtccttctt gggcgtgggc
     2701 ttgccgccgg ccgcgaactt ggccacggcg ccgaggccgg tggccttcga agcctggccg
     2761 cccgtgttcg agtagacctc ggtgtccatc acgaggatgt tcacgttgcg gccgctggcg
     2821 agcacgtggt ccaggcctcc gaagccgatg tcgtaagccc agccgtcgcc gcccacgatc
     2881 cagaccgtct tgcggaccag ggtgtcgagc agtccgagca ggtcgacgga ttcggggtcg
     2941 gtgcgtccct ggagcttgtc gcggagacgg tccacgcggg agcgctgcgc gccgatggcc
     3001 gcctcgtccg gctggtcggc cgc
//
LOCUS       DBNHQZ010000088         3347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_702951, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000088 DBNHQZ010000000
VERSION     DBNHQZ010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_702951"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="AC16YU_02240"
     CDS             complement(<1..2224)
                     /locus_tag="AC16YU_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GPI anchored serine-threonine rich family
                     protein"
                     /protein_id="HIR6064067.1"
                     /translation="MITGTARAVQTTGDPSAGAGYAETLVGSLRLRQDKSYPPPATVY
                     ILESGWVTWSATGQIGDCSVTGVSTRVDLQPSEGQIQIGPDGVYSGGVTSSALGSAAE
                     LRLNCPDGSTEVPPSLITVFGVGPFDGFTVSEDGRRLEGSLSYPGLDQSWNLARCVSL
                     HITAPTADEHLVPGETYTITWAADGVEVVNLDLRTRAGAVSARIADHLPAVEERYAWK
                     VPDSLARRAQIHIEDAAIPEIGDTSDEFRIKGYVLARVRDEEYERFEPARHGWKAENS
                     DGNWDPPTYAETGQFCYRCQPPGTDPFAQKPYPFYQFYLAEMLPPRFGDFPSFVRAFG
                     GGPFYWADPRSPEPAAYRTSGLFFWVAKSGPDWGGSCSGMSVSALMAFSKPGAFGARF
                     PAIGARERLFDAIPGASVPDPVRASVNALFQYWHGTAATRHTAANLAKRPRATLENLK
                     EMLLDPDDTHAHDAYLYIANATGSALGVRAHAVAPILMKKTSEEPTRYDLEVYDPNAP
                     GQRSKIQIDSTADKWAYPGQYDGSGSFFLMDPTANYFPLAGFPGLAAGKNHAVDDLEI
                     VPSPWASITIEGSAGRIGYQDGRSFSTIPDREPVVVPSGYPSYPTPYLVGPDAYQVTQ
                     TSYPEPLASLLLIDQGRFYSARRADASPDQTDRFTVGSGLRIRNDDPTAKSWSYSAVL
                     PGETHDRVFDVTGLDLAQGDSVDLSVNAADELRIHNPGSARTYGLTVRMESASGVVSR
                     H"
     gene            complement(2805..3128)
                     /locus_tag="AC16YU_02245"
     CDS             complement(2805..3128)
                     /locus_tag="AC16YU_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR6064068.1"
                     /translation="MAGRFHVYVIELDPAVARRRRFREANPRMRPGLPPLYVGSSVQP
                     PDERFDQHKEGYKANRYVREFGVRLRPDLYEQYNPIPSRADALDIEEYLASRLRTKGH
                     GVWQH"
BASE COUNT          524 a         1094 c         1199 g          530 t
ORIGIN      
        1 ggtggcggct cacgacgccc gatgccgact ccatccgcac cgtgaggccg tacgtccggg
       61 cgctgccggg attgtggatg cggagttcat cggcggcatt caccgacagg tcgacggagt
      121 ctccctgtgc caggtcgaga ccggtcacat cgaagacgcg atcgtgcgtc tcaccgggca
      181 ggacggccga atacgaccag gacttcgccg tcgggtcgtc gttgcggatg cgcaggccac
      241 tgccgaccgt gaaccggtcg gtctggtccg gcgacgcgtc cgcgcgacgc gcggagtaaa
      301 agcgaccctg atcgatcagc aagaggctcg cgagcggctc cgggtacgac gtctgcgtga
      361 cctggtacgc atccggtccg accaggtacg gcgtcgggta cgagggatag ccggagggaa
      421 cgacgaccgg ctcgcgatcg gggatcgtgc tgaacgagcg gccgtcctgg tatccgatcc
      481 gtcccgcaga tccctcgatc gtgatcgaag cccacggaga gggcacgatc tccaggtcgt
      541 cgaccgcgtg attcttgccg gcggcaaggc cgggaaagcc ggccaggggg aagtagttgg
      601 ccgtgggatc catcaggaag aacgaaccgg agccgtcgta ctgccccggg tacgcccatt
      661 tgtcggcggt cgagtcgatc tggatcttgc tgcgctgtcc cggagcgttg ggatcgtaca
      721 cctcgaggtc gtaccgcgtg ggttcctccg aggtcttctt catcaggatc ggggccacgg
      781 catgggccct gaccccgagc gccgagcccg tcgcgttggc gatgtagagg tacgcgtcgt
      841 gggcgtgggt atcgtccggg tcgagcagca tctccttcag attctccagc gtcgctcgtg
      901 ggcgcttggc caggtttgcc gcggtgtgcc gggtagcggc agtcccatgc cagtactgga
      961 agagggcgtt gaccgaagcc cgcacgggat cgggcacgct cgcccccggg atggcgtcga
     1021 agaggcgctc ccgggcgccg atcgccggaa accgggctcc gaaggcgccg ggcttggaga
     1081 acgccataag cgccgacacg ctcatgccgg agcacgagcc gccccagtcc gggccggact
     1141 tcgcgaccca gaagaagagg ccggaggtcc tgtaggcggc cggctccggc gatctgggat
     1201 ccgcccagta gaatggtcca cctccaaaag cgcgcacgaa cgaggggaag tccccgaatc
     1261 gtggcggcag catttcggcg aggtagaact ggtagaaggg gtatggcttc tgggcgaacg
     1321 gatcggtgcc gggaggctgg caccggtagc agaactgtcc ggtctccgcg tacgtcggcg
     1381 gatcccagtt gccgtccgaa ttctcggcct tccagccgtg ccgtgccggc tcgaaccgct
     1441 cgtactcctc gtcccggacc cgggcgagga cgtacccctt gatgcggaac tcgtccgacg
     1501 tatcgccgat ctccgggatc gcggcgtcct cgatgtggat ctgggcgcgg cgcgccaggg
     1561 agtcgggcac cttccacgcg taccgctcct cgacggccgg caggtggtcg gctatccggg
     1621 cgctgacggc gccggcccgg gttctgaggt ccaggttcac cacctcgacg ccatccgccg
     1681 cccaggtgat agtgtaggtc tcgcccggga ccaggtgttc gtcggccgtc ggcgcggtga
     1741 tgtggagcga cacgcaacgg gcgaggttcc agctctgatc gaggcctggg tacgagaggc
     1801 tgccctcgag acggcggccg tcctcggaga ccgtgaaccc gtcgaacggg ccgacgccga
     1861 acaccgtgat cagggacgga gggacctcgg tcgatccgtc cggacagttc aggcggagct
     1921 ccgcggcgct tcccagggcg gaagacgtga cccctccgga gtagacgccg tcggggccga
     1981 tctggatctg gccttcggac ggctggagat ccacccgggt gctgacgccc gtgacggagc
     2041 agtcgccgat ctggccggtc gcgctccagg tgacccagcc gctctccagg atgtagacgg
     2101 tcgccggggg tggatacgac ttgtcctgcc ggagacggag gctgccgacg agagtttccg
     2161 cgtatccggc accggccgac ggatcacccg tcgtctgcac cgcgcgcgcg gtgccggtga
     2221 tcaattcgca ggcgtcggac ggtccggccg tcgaccgggc cgagcgcccg acaaggggtg
     2281 actgagccag ggcggctggc ccgtgcgcga gtagaaggag caggacggcg atccgctgga
     2341 cccgccgggg catcgacgtg gatccgcaag gcatacgagg tgtgcgagaa gcgcattccg
     2401 gcgtctgcac ggtgtccgga atgcctattc gggagtcggc ccgcgtctga caccgctcat
     2461 ccagccaccc ggaccctggt ccggaccgtg ccgttccccg gaacttgccg ggtcgcaggg
     2521 gtgcgggcgc acgcgcgccc gacgatgatc cgcacccggg gcaggcgcta cccccgtacg
     2581 gtcaccggct ggaaggtgat cgtcaaccgg actggacggg ctggcacatg gcgaccctcc
     2641 gggatgaaaa gggggtagat gcggcgaagt gtatgcgccg tcggagtcca ttccatgccc
     2701 gggacaactc tattctctgt gtaggacgat gtcctacacg atcttctggc gctgaccggc
     2761 gacggggcag gcactggtgg cgcccgatcc ggaaccggcc gaattcagtg ctgccacacg
     2821 ccgtgcccct tcgtgcggag acgcgaggcc aggtattcct cgatgtcgag cgcgtccgcc
     2881 cgggacggga tcgggttgta ctgctcgtac aggtccgggc ggaggcgaac gccgaactcc
     2941 cgcacgtagc ggttggcctt gtacccctcc ttgtgctggt cgaaccgctc gtcgggcggc
     3001 tggaccgagc tgcccacgta gagcggaggc agcccgggcc tcatgcgggg gttcgcctcg
     3061 cggaatcgcc ggcgcctggc gacggcgggg tcgagctcga tgacgtatac gtggaagcgg
     3121 ccggccatgg tcagatcatt cctcattgcc tcccacggcg gctaggcatg gtcggttcag
     3181 acggccgtcc cgtccacctc cgacacgtcc accccgcgcc actcggccac cttgcctacg
     3241 atcgccgcgc gtacgcgctc ctgcacggcc cgggcgtcgt ccgggcccag gccctcgact
     3301 gggacggggt cgaacacgtg gatccggatc gtccgcgggg ggccgaa
//
LOCUS       DBNHQZ010000089         3967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_160032, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000089 DBNHQZ010000000
VERSION     DBNHQZ010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_160032"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC16YU_02250"
     CDS             <1..780
                     /locus_tag="AC16YU_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR6064069.1"
                     /translation="TRHEDGWIYGLFCTERRDPDATPGDYSAAVAQCGIARTHDLDTW
                     ERLPDLRTHSPQQRNVVLHPEFVEGRYALYTRPQDRFIDAGKGGGIGWGLTDSMEDAE
                     IDEETIVDGREYHTIKEAKNGLGPAPIWTDYGWLHLAHGVRHTASGLRYVLYLFMTAL
                     DDPTRIIHSPAGQFLGPVGEERVGDVSNVVFSNGWIARPDGKVYIYYASSDTRCHVAV
                     TTLERLLDHVMQAPPDAGRSAANVALRNELIERNRGFLETN"
     gene            784..2025
                     /locus_tag="AC16YU_02255"
     CDS             784..2025
                     /locus_tag="AC16YU_02255"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR6064070.1"
                     /translation="MPSHPGLDALRAGMARELTERLLPWWMSRAPDPARGGFVGRITG
                     AGEAQLDAPRGSILNARILWAFSAAYRMFGREGHREMADRAFNWFDRHFVDPDWGGVY
                     WMVCRDGRPTDERKHAYAQSFAIYALAEYCRATGDERALRRAVEVFTLLEDHARDRRH
                     GGYLEAFSRAWERLEDQRLSEKDADVPKTMNTHLHLLEAFTGLYGVWPDGWAGARLRE
                     LVDVFLDRVVDPETGHVLLYFDEDWTPRSRMRSYGHDIEASWLLLEAADALGDPVLRR
                     RVLPVSLRLVDGVLREGVDADGGLFNERREDGRIDHDKHWWPQAEAVVGFVNAWQETG
                     QERYLAAAESAWAFTERFILDPVGGEWFWRVDRSGRVYEEEDRLGPWKCPYHNVRSCM
                     EIMRRAELVPDDDAPRGATRV"
     gene            2123..>3967
                     /locus_tag="AC16YU_02260"
     CDS             2123..>3967
                     /locus_tag="AC16YU_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HIR6064071.1"
                     /translation="MALRFLSAAAAVAVCIPQPHAAQAQPGPIAVLSVNAGAHVRVDT
                     PIGASLVGVPGAGTTALVLVEVTDGIERPVRSQVDGSLLRWVLDGETAPGAVRTFELR
                     ASPGSAPQGRVGLSDDGAALTLGIGEHPVLSYRYAVQPAPEGAEPVFARSGFIHPLWS
                     PGGAVLTRIQPPDHLHHYGIWNPWTSTEFEGRAVDFWNIGSGQGTVRARDILERRSGP
                     VAGGFRAALEHVAFGGPAGETIALDETWDVTAWDVRHGAGPWIVDFVSTLSPASGVPL
                     TVKGYRYQGFSIRATERWSDENSTLLTSEGLDKSTGNATRARWVDLGGETDVPAGRSG
                     LVMMTHPANFNHPEHLRIWPTGMNEGRENVFVNFNPAQDRDWELEPGGTYSLRYRLVV
                     YDGAMPQDVAERHWAEFADPPRVTVHPTSLRGARVFVYTRNGAGYVHDNIASSVAAIR
                     RLGEEHGFSVESGDDPAAFTPVNLARFDALVFSNTNNEAFDTDDQREALQAYVRAGGG
                     FVGIHSASGSERDWPWYSALVGGNFERHAPQQDFTVQVVDASHPSTAFLPRRWEIESD
                     ECYYHDELNPGIRVLLAADLSTVDDPDGGSFPGTLFADRYPVAWYQAFD"
BASE COUNT          592 a         1382 c         1416 g          577 t
ORIGIN      
        1 acgcggcacg aggacggctg gatctacgga ctgttctgca ccgagcgccg ggatccggac
       61 gcgaccccgg gggactactc ggccgcggtg gcgcagtgcg gcatcgcgag gacccacgac
      121 ctcgacacct gggagcgcct ccccgacctc aggacccatt cgccccagca gcgcaacgtg
      181 gtgctgcacc ctgaattcgt ggaagggcgg tacgccctgt acacccgccc gcaggaccgg
      241 ttcatcgatg ccgggaaggg cggaggcatc ggctggggcc tgaccgactc catggaggac
      301 gcggaaatcg acgaggagac catcgtcgac ggacgcgaat accacaccat caaggaagcg
      361 aagaacgggc tgggcccggc gcccatctgg acggactacg gctggcttca cctggcccac
      421 ggcgtccgcc acacggcctc ggggctccgc tacgtgctgt acctgttcat gacggcactc
      481 gacgacccga cacgcatcat ccacagcccg gccggccagt tcctgggccc ggtcggagag
      541 gagcgggtcg gcgacgtctc gaacgtcgtc ttctcgaacg ggtggatcgc ccgccccgac
      601 gggaaggtgt acatctacta cgcatcgtcc gacacccgat gccacgtggc ggtgaccacg
      661 ctggagcggc tgctggacca cgtgatgcag gcgcccccgg acgcgggccg ctccgctgcc
      721 aacgtcgccc tccgcaacga gctcatcgaa cggaatcggg gcttcctgga aaccaactga
      781 gacatgccct cgcatcccgg gttggatgcc cttcgcgccg gcatggcgcg cgagctgacc
      841 gagcgtcttc tcccctggtg gatgtcgagg gcgcccgacc cggcgcgggg cggtttcgtt
      901 ggtcgcatca ccggtgcggg cgaggcgcag ctcgacgcgc cgaggggcag catcctgaac
      961 gcccgcatcc tctgggcctt ctcggccgca taccgcatgt tcggccggga gggtcaccgg
     1021 gagatggccg accgggcctt caactggttc gaccgtcatt tcgtggaccc ggactggggc
     1081 ggcgtgtact ggatggtgtg ccgggacgga aggccgacgg acgaacggaa gcacgcgtat
     1141 gcgcagagct tcgcgatcta cgcgctcgcg gaatactgcc gggcgacggg tgacgagcgg
     1201 gcgctgcgcc gcgccgtgga agtattcacc ctgctcgagg accacgcgag ggaccgtcgg
     1261 cacggggggt acctggaggc cttctcgcgc gcctgggagc gcctcgagga ccagcgcctg
     1321 agcgagaagg atgcggacgt gcccaagacc atgaacacgc acctgcacct gctcgaggcc
     1381 ttcaccgggc tctacggcgt ctggccggac ggctgggccg gggcccgcct ccgcgagctg
     1441 gtcgacgtct tcctggaccg ggtcgtggac ccagagacgg ggcacgtgct cctgtatttc
     1501 gacgaggact ggacgccgcg gtcgcgcatg cgctcgtacg gccacgacat cgaggcgagc
     1561 tggctcctcc tcgaggcggc cgacgccctg ggtgatcccg tcctgaggcg gcgcgtgctt
     1621 ccggtctcgc tccgcctcgt ggacggcgtg ctccgtgagg gggtggacgc tgacggcggg
     1681 ctcttcaacg agcgccgcga ggacggccgg atcgaccacg acaagcactg gtggccccag
     1741 gccgaggccg tcgtggggtt cgttaacgcg tggcaggaga cggggcagga gcgctacctg
     1801 gcggccgcgg agtcggcgtg ggccttcacg gagcgcttca tcctggaccc ggtgggcggg
     1861 gagtggttct ggcgggtgga ccggtcgggc cgcgtgtacg aggaggagga caggctgggc
     1921 ccctggaagt gcccctacca caacgtccgc tcgtgcatgg aaatcatgcg ccgggccgag
     1981 ctggttccgg acgatgacgc cccgcgcggg gcgacgcgcg tctgagggga ggcgcaggtc
     2041 cctccgattg gagatattgt accggcgtac ccgcggcgcg ccggtcgccg tgaaggtccg
     2101 cccgcaccct ccgaggcaag agatggccct ccggttcctg tccgcggcgg cggccgtcgc
     2161 cgtctgcatc ccgcagcccc acgccgccca ggcccagccg ggtccgatcg ccgtgctgtc
     2221 ggtgaacgcc ggggcgcacg tgcgtgtcga cacgcccatc ggtgcgtccc tcgtcggcgt
     2281 cccgggagcc gggacgaccg ctctcgtgct cgtcgaagtc acggacggca tcgagcgtcc
     2341 ggtgcggtcg caggtcgacg gctctctcct gcggtgggtg ctcgacgggg agaccgcccc
     2401 gggcgcggtg cgcacgttcg agctccgcgc ctcccccggt tcggcaccgc aagggcgggt
     2461 agggctctcc gacgacgggg cggcgctcac cctgggcatc ggcgagcacc ctgtcctgtc
     2521 gtaccggtac gcggtacagc cggcgccgga gggcgccgag cccgtcttcg cccgttccgg
     2581 tttcatccac ccgctctggt cgcccggagg cgccgttctc acccggatcc agccgcccga
     2641 ccacctgcac cactacggca tctggaatcc gtggacgagc acggagttcg aggggcgcgc
     2701 ggtcgacttc tggaacatcg ggagcggcca gggcacggtg cgggcgaggg acatcctcga
     2761 gcggcgctcg ggtcccgtgg ccggcgggtt ccgcgccgcc ctcgaacacg tggccttcgg
     2821 cgggccggcc ggcgagacga tcgcgctcga cgagacgtgg gacgtgaccg cctgggacgt
     2881 gcgccatggc gccgggccgt ggatcgtcga tttcgtctcc acgctaagcc ccgcgtccgg
     2941 cgtgccgctc accgtgaagg gctatcgcta ccagggcttc agcatccgcg caacggagcg
     3001 gtggagcgac gagaactcga cgctcctgac gtccgaaggg ctcgacaagt cgaccggcaa
     3061 cgccacccgc gcccggtggg tcgacctcgg cggcgagacc gacgtgccgg cgggacggtc
     3121 gggcctcgtg atgatgacgc acccggccaa cttcaaccat ccggaacacc tgcgcatctg
     3181 gccgaccggc atgaacgagg gccgggagaa tgtcttcgtg aacttcaatc cggcgcagga
     3241 ccgcgactgg gagctcgagc cgggggggac gtacagtctg cgctaccggc tcgtggtgta
     3301 cgacggggcg atgccgcagg acgtggccga gcgccactgg gccgaattcg ccgacccgcc
     3361 ccgcgtgacg gtccacccga cgtcgctccg gggcgcacgg gtcttcgtct acacccgcaa
     3421 cggcgcgggc tacgtgcacg acaacatcgc ctcctcggtt gcggccatac gccgcctggg
     3481 cgaggagcac ggattctcgg tggaatccgg ggacgatccc gcggccttca cgcccgtgaa
     3541 cctcgcgcgc ttcgacgcgc tcgtcttctc gaacacgaac aacgaggcct tcgatacgga
     3601 cgaccagcgc gaggccctgc aggcgtacgt ccgtgccggg ggaggtttcg tgggcatcca
     3661 ctcggccagc ggctcggagc gcgactggcc gtggtactcc gcactggtgg gcggcaactt
     3721 cgagcggcac gccccccagc aggacttcac ggtccaggtc gtcgacgcgt cccatccctc
     3781 gacggccttc ctgccgcgcc ggtgggagat cgaaagcgac gagtgctact accacgacga
     3841 gctgaacccg ggaatccggg tgctgctcgc ggccgacctc tcgacggtgg acgaccccga
     3901 cggcggttcc ttccccggga ccctcttcgc cgaccggtac ccggtggcct ggtaccaggc
     3961 gttcgac
//
LOCUS       DBNHQZ010000090         3204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1922623, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000090 DBNHQZ010000000
VERSION     DBNHQZ010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1922623"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /gene="fucP"
                     /locus_tag="AC16YU_02265"
     CDS             <1..1168
                     /gene="fucP"
                     /locus_tag="AC16YU_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HIR6064072.1"
                     /translation="NFEGSLVQFAFYGGYFAMAFPAALFIKRYSYKAGILVGLALYAG
                     GALLFIPASWSMAFAPFLVAYFILTCGLSFLETSANPFVLSMGSIGTATRRLNLAQAF
                     NPVGSLTGMFVAREFILARLDPATAAERAALAPAELDALTASDLAIVRAPYVAIAGVI
                     IVVAVLVWRTRMPGSVGGAGRLDVMPTLRRLLADRSYVGGVVAQFFYIGAQITCWTFI
                     IQYGTEVLMQARGLTESQAEVAAQGYNMVAMVIFLASRFVCTFLLRYVSPGALLGVLA
                     AAGFVLVAGTIALPGVTGLYCLVGVSACMSLMFPTIYGIALRGMGDDAKLGAAGLIMA
                     ILGGSVLPPVQAAIMDGSGLAGLSAVRLSFVVPLLCFVVVARYGFRTRRSETAA"
     gene            complement(1181..2746)
                     /locus_tag="AC16YU_02270"
     CDS             complement(1181..2746)
                     /locus_tag="AC16YU_02270"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR6064073.1"
                     /translation="MHRVLSALLLVLLAVAPAGAQNTPIGQARLEFLPDTTGPAISRH
                     IYGQFAEHLGRNIYGGFWVGEGSSIPNVRGIRTDVVEALREVGVPNLRWPGGCFADIY
                     HWRDGIGPKEERPAVPNRFWGRALEDNAFGTHEFMDLAEQLGAEPYIAGNLGSGTVQE
                     MADWAEYLTRDDGSPMAELRRRNGRDEPWPVHLFGVGTESWGCGGNMRPEYYADLFRQ
                     YATYLREYGRGLSLVAAGPSAGYGGYGDDLRWTEILMREAGWLMGGYSLHYYTVTHNW
                     RRKGSATEFDESEWFLTLKKATWIDSVITAHGRVMDRFDPHKRVAMIVDEWGTWHDVP
                     PDTAGHTLYQQNTLRDALVAGITLNSFNNHADRVRVANLAQTVNVLQSMILTQGNRMV
                     RTPTYHVFSLFRGHHDATLLPTRIEAPDYVFGDDAMPALSASASVDSTGAIRVTVTNA
                     DPDQGYSLRVPVEPHHRFFLARIVTAETMQAHNTFDEPDRVRVQGYREYRRSGDHVLL
                     LVPAKSIVAFEIR"
     gene            complement(2797..>3204)
                     /locus_tag="AC16YU_02275"
     CDS             complement(2797..>3204)
                     /locus_tag="AC16YU_02275"
                     /inference="COORDINATES: protein motif:HMM:NF053331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannanase"
                     /protein_id="HIR6064074.1"
                     /translation="KAIGYLADHVAIAERLGKPIVLEEFGLSRDGGSYEPSAPTTWRD
                     RFFRIVFEAALKLAEERSALAGLNVWSWSGEGRGVRPGEVWLPGEPLTGDPPHENQGW
                     YSIYLEDTSTIDLLGTYARRVSALAGTGPEAER"
BASE COUNT          428 a         1087 c         1135 g          554 t
ORIGIN      
        1 caatttcgag ggctccctcg tccagttcgc cttctacggg ggatacttcg cgatggcctt
       61 tccggccgcc ctcttcatca agcgctactc gtacaaggcc gggatcctgg tggggctcgc
      121 gctctacgca ggcggggcgc tgctcttcat tccggccagc tggtcgatgg ccttcgcgcc
      181 gttcctggtc gcgtacttca tcctgacctg cggcctgtcg ttcctggaga cgagcgcgaa
      241 cccgttcgtc ctgtcgatgg gctcgatcgg gacggcgacc cgacggctga acctggcgca
      301 ggccttcaac cccgtaggct cactgacggg gatgttcgtg gcccgggagt tcatcctcgc
      361 ccggctcgat cccgcgacgg ccgcggagcg ggcggcgctc gcgccggccg agctcgacgc
      421 gctcacggcg tccgacctgg ccatcgtgcg cgcgccgtac gtggccatcg ccggggtgat
      481 catagtggtc gcggtcctcg tctggcgcac ccggatgccg gggtcggtcg gcggcgcggg
      541 ccggctcgac gtgatgccca cgctccgccg gctcctggca gaccggagct acgtgggggg
      601 tgtcgtggcg cagttcttct acatcggcgc gcagatcacg tgctggacct tcatcatcca
      661 gtacgggacc gaggtgctca tgcaggcgcg cggcctgacc gagtcgcagg ccgaggtggc
      721 cgcgcagggc tacaacatgg tggccatggt catcttcctg gccagtcggt tcgtgtgcac
      781 gttcctgttg cgctacgtga gtcccggggc gctgcttggg gtactcgccg cggccgggtt
      841 cgtgctcgtc gccggcacga tcgcgctgcc cggcgtcacc gggctgtatt gcctggtggg
      901 tgtgtcggcg tgcatgtccc tcatgtttcc cactatctac gggatcgcgc tgcggggcat
      961 gggcgacgac gcgaagctgg gcgcggccgg cctgatcatg gcgatcctcg gcggttcggt
     1021 gctgccgccc gttcaggccg ccatcatgga cggctcgggg ctcgccggcc tgtcggcggt
     1081 acgcctctcc ttcgtggttc cgctcctgtg cttcgtggtc gtggcccggt acggcttcag
     1141 gacccgccgg tccgaaaccg cggcctgacg cagccggcga tcaccggatc tcgaaggcga
     1201 cgatggactt ggcgggaacg agaaggagca cgtggtcccc gctgcgccgg tattcccggt
     1261 agccctgcac ccgaacccgg tccggctcgt cgaacgtgtt gtgggcctgc atggtctccg
     1321 ccgtcacgat gcgcgcgagg aagaaccggt ggtgcggctc caccggaacg cgcagcgagt
     1381 acccctggtc ggggtccgcg ttcgtgaccg tgacgcggat cgcgcccgtc gagtcgacgg
     1441 acgccgaggc cgagagggcg ggcatcgcgt cgtcaccgaa gacgtagtcc ggggcctcga
     1501 tccgggtggg caggagcgtg gcgtcatggt ggcccctgaa gagcgagaac acgtggtacg
     1561 tgggagtccg caccatgcgg ttgccctgcg tgaggatcat cgactgcagc acgttcaccg
     1621 tctgggcgag gttcgcgacg cgcacgcggt cggcgtggtt gttgaagctg ttcagtgtga
     1681 tgccggcgac cagggcgtcg cgcagggtgt tctgctggta cagggtgtgc ccggcggtgt
     1741 cgggaggcac gtcgtgccac gtgccccact cgtcgacgat catggccacg cgcttatgcg
     1801 gatcgaagcg gtccatgacg cggccgtggg ccgtgatgac ggagtcgatc cacgtcgcct
     1861 tcttcagcgt gaggaaccac tcggactcgt cgaactccgt cgccgagccc ttgcggcgcc
     1921 agttgtgcgt caccgtgtag tagtggagcg agtagccccc catgagccag ccggcctcgc
     1981 gcatcaggat ctcggtccag cgcaggtcgt cgccgtatcc gccgtacccc gcgctcgggc
     2041 ccgcggccac gagcgagagg ccgcgcccgt actcgcggag gtaggtcgca tattgccgga
     2101 agaggtccgc gtagtactcc ggccgcatgt tgccgccgca gccccagctc tcggtcccca
     2161 cgccgaacag gtggacgggc cagggctcgt cgcggccgtt gcggcggcgc agttccgcca
     2221 tggggctgcc gtcgtcccgg gtcaggtact cggcccagtc ggccatctcc tgcaccgttc
     2281 ccgatccgag attccccgcg atgtacggct cggcgcccag ctgctcggcc aggtccatga
     2341 actcgtgcgt cccgaacgca ttgtcctcga gagcgcgccc ccagaaccgg ttgggcaccg
     2401 ccggcctctc ttctttcggg ccgatgccgt cccgccagtg gtagatgtcc gcgaagcacc
     2461 cgccgggcca cctcaggttc ggcacgccca cctcccggag ggcctccacg acgtccgtcc
     2521 ggatgccccg cacgttcggg atggacgagc cctcgcccac ccagaagccg ccgtagatgt
     2581 tgcggcccag gtgctcggcg aactgcccgt agatgtgtct cgagatggcc ggtcccgtgg
     2641 tgtccggcag gaattcgagc cgggcctgcc cgatcggggt gttctgcgcg ccggccggcg
     2701 cgacggcgag gagcacgagg aggagggcgg agaggacgcg atgcatggcg cggggaggat
     2761 cgtgaaatgg caatctattg caccccacgg tgcgattcag cgttcggcct ccggacccgt
     2821 gcccgcgagg gcggaaacgc ggcgcgcgta ggtgcccagg aggtcgatcg tggaggtgtc
     2881 ttcgagatag atcgagtacc acccctggtt ctcgtggggc ggatcaccgg tgagcggctc
     2941 gccgggcagc cagacctccc cgggccgtac gccgcggccc tcccccgacc aggaccagac
     3001 gttgagcccg gcgagcgccg agcgctcctc ggcgagcttc agggcggcct cgaagacgat
     3061 ccggaagaag cggtcccgcc acgtggtcgg ggccgacggc tcgtaggacc ctccgtcgcg
     3121 cgacaggccg aattcctcga gcacgatggg tttgccgagc cgctccgcga tggccacgtg
     3181 gtccgccagg tacccgatgg cctt
//
LOCUS       DBNHQZ010000091         4651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3004763, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000091 DBNHQZ010000000
VERSION     DBNHQZ010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3004763"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16YU_02280"
     CDS             complement(<1..255)
                     /locus_tag="AC16YU_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064075.1"
                     /translation="MPSSAMRLLLALTVTYALVGIGGRDARAQDHDAHAAGHADQADA
                     ARSLKLLMVTLGQDMQRIADGNWHEDFVMIGAGARAIASHP"
     gene            474..1658
                     /locus_tag="AC16YU_02285"
     CDS             474..1658
                     /locus_tag="AC16YU_02285"
                     /inference="COORDINATES: protein motif:HMM:NF017727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HIR6064076.1"
                     /translation="MIPIDIGPSRPAGPPFLQDGFRPFFLGATAWAVLGMLPWWASYA
                     LAVPMPRAEVVGMQWHAHEMIFGFAAAVVAGFLLTAVRNWTGRPTLSRGPLLALFAAW
                     LAARGTFTAGWIPVWIPAAADFAFGCGLLVAIAVPIRDAGQLGRQAGILSKVAMLPVA
                     SALFFAGTMGWIAPGIRWGLYLGFFAVLGLVLSVGRRIMPSFIAVGTGTKTPLRNRAW
                     VDRSALVLYGILVVAMVFVPVGALVAASAALLALVHGIRAAGWYAPGIWKRPLLWTLY
                     VSYLFTIAGFGLLALSHWAPLPPLVGIHALGAGGIGLITAGMMARVSLGHTGRGIANL
                     PRGTAAAFGLLLAAAIVRVAFPIAAPGLAWEWVIASQSLWMLGFALLAARFLPVWTGP
                     SA"
     gene            complement(1714..4398)
                     /locus_tag="AC16YU_02290"
     CDS             complement(1714..4398)
                     /locus_tag="AC16YU_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6064077.1"
                     /translation="MRHVLLAALLVLAVRSASAQHTVSGIVTDGANEPLIGVNIAEKG
                     TFNGTTTGVDGRYRLEVGSRDATLVFSYVGFQRQEIAVGGRPQIDVVLVEGLTLGEVQ
                     VVGSRSVNRSVTDSPVAVDVIDVGDLAATTGRAEVNQILQVAAPSFNATKQSGSDGAD
                     HIDPASLRGLGPDQTLVLVNGKRRHQSSLVNVFGTRGRGNTGTDLNAIPASAIKRVEI
                     LRDGASAQYGSDAIAGVINIVLKDRTDGLSASLTRGAYSTAIGDGYAEATGETIYNVK
                     GENRLDGEDRSFDGTTTKLDLNYGIGLGDNGGFANVTTEFLTRDRTLRPGFSWRKGYG
                     TAAVDGFAFLVNSALPLGADTEVYAFGGRSFRDTDAYAFSRDGFEDGDNRAVPSLYPD
                     GFTPRITSNITDVSATVGVRHALAAGWRADFANTYGKNDFHYFIRGTNNASLGDASPT
                     DFDAGGHSLSMNTTNLDLSRYFADVAEGLNVAFGLEYRTETFAIFAGEEASYAIYDEN
                     GVAVTNPAIQEPAEDSNGDQLPGGSQGFPGYSPANVVDRGRSNFGVYADAELDATSEL
                     LVGGAVRYERYSDFGETLNFKATGRYRLSPALSVRGSVSTGFRAPSLAQIHYNLVFNN
                     IVAGASLRTLLASNTSTVTKAFGIDRLDEETAVNGSVGFTFESRGFSATVDAYSIAVD
                     DRIILTDNFDASDLDIGVEAAQFFANGVDTRTTGVDIVVGYRTNLGPGRLNVGLAGNV
                     NDLEIQRINNGELNPFTFFGPFSQAYLEAAAPDYKFGLNVGYAASRFDVQLSLTQFSE
                     VQLQDFQWVDTPATNQAEADALYEVATDTYDAAMTVDLSVSYIVTPHVRLTVGGNNLF
                     NTYPTPQFDAWTDQGGFNDSVQMGSDGTYFFGRIAVDL"
BASE COUNT          640 a         1593 c         1602 g          816 t
ORIGIN      
        1 gggatgcgac gcgatggcac gcgcccctgc gccgatcatc acgaagtcct cgtgccagtt
       61 gccgtccgcg atccgctgca tgtcctgtcc cagcgtgacc atgagcagct tcagcgaacg
      121 cgcagcgtcg gcctggtccg cgtggcccgc ggcgtgggcg tcatggtcct gcgcccgggc
      181 gtcccggccc ccgatgccga cgagcgcgta agtcacggtc agggcgagca gcaggcgcat
      241 ggcggaggac ggcatggtgg gacgacgcgt tcaatgtggg tgattcggca ggcgacgggt
      301 cactcccgag tcgacaaacc gcgcgggact ccccgaggat tcatctgcgc ggcgcccgta
      361 ttgactacaa ccgagtcgcc aatcttctat tttcctttcg cggcatggct ccgcctgcgc
      421 ggcgggacga cggcgccccg tcgcccgccg acatccccat cgcacgatcc gccatgatcc
      481 ccatcgacat cggcccttcg agacccgccg gcccgccctt ccttcaggac ggtttccggc
      541 ctttcttcct cggcgccacg gcatgggccg tgctcggcat gctgccctgg tgggcgtcgt
      601 acgcgctggc cgtgcccatg ccccgcgccg aggtcgtcgg catgcagtgg catgcgcacg
      661 aaatgatctt cgggttcgcg gccgcggtcg tggcggggtt cctcctcacg gccgtccgca
      721 actggacggg ccgacccacc ctgtcgcgcg gaccgctcct ggcccttttc gcggcgtggc
      781 tggcggcgcg gggcaccttc acggccgggt ggatccccgt ctggatcccg gcggcggcgg
      841 acttcgcttt cggatgcggc ctcctggtcg ccatcgccgt tccgatccgc gacgccggac
      901 agctgggccg ccaggccggg atcctgtcca aggtcgccat gctgcccgtc gccagcgcac
      961 tgttcttcgc gggtacgatg ggctggatcg cacccggtat ccggtggggc ctttacctgg
     1021 gcttcttcgc cgtgctggga ctcgtgctct ccgtcggacg gcgcatcatg cccagcttca
     1081 tcgccgtcgg caccggcacg aagaccccgc tgcgaaaccg cgcctgggtg gacaggtccg
     1141 cgctcgtgct gtacgggatc ctggtcgtgg cgatggtctt cgtgcccgtc ggcgcgctcg
     1201 tcgccgcgtc ggccgcgctc ctcgccctgg tccacgggat ccgggcggcc ggctggtacg
     1261 cgcccgggat atggaagcgg ccgctgctct ggacgctcta cgtctcctac ctgttcacca
     1321 tcgccggatt cgggctcctg gccctgagcc actgggcccc gctccctccg ctcgtcggca
     1381 tccacgcgct cggtgctggg ggtatcggac tcatcacggc cggcatgatg gcccgcgtct
     1441 cgctggggca caccggacgg ggcatcgcga acctgccccg tggcacggcc gcggcgttcg
     1501 gactgctgct cgcggccgcg atcgtgcggg tcgccttccc gatcgcggcg cccggcctgg
     1561 cgtgggaatg ggtgatcgcg tcccagtcgc tgtggatgct ggggttcgcg ctgctcgcgg
     1621 cgcgcttcct gcccgtctgg acgggcccct ccgcctgaac ccgaggccgg acaggccccg
     1681 gtggtcggga ccgcccgagc cgtccgcgcg gacctacagg tcgaccgcga tccggccgaa
     1741 gaaatacgtg ccgtcggagc ccatctgcac ggagtcgttg aacccgccct ggtcggtcca
     1801 ggcgtcgaac tgcggggtcg ggtaggtatt gaacaggttg ttgccgccaa cggtgagccg
     1861 cacgtggggc gtgacgatgt agctcacgct caggtcgacc gtcatggccg cgtcgtacgt
     1921 gtcggtcgcg acctcgtaga gagcatcggc ctccgcctgg ttggtcgccg gcgtgtcgac
     1981 ccactggaag tcctgcagct gcacctcgct gaactgggtg agcgagagct ggacgtcgaa
     2041 gcgggacgcg gcgtacccga cgttcagccc gaacttgtag tcgggtgcgg ccgcttccag
     2101 gtaggcctgc gagaacgggc cgaagaacgt gaaaggattg agttcgccgt tattgatgcg
     2161 ctggatctcg aggtcgttga cgtttcccgc caggcccacg ttcaatcggc ccgggccgag
     2221 gttcgtgcgg tagccgacga cgatgtccac gccggtggtc cgcgtgtcga cgccgttcgc
     2281 gaagaactgc gccgcctcga ccccgatgtc caggtcggac gcgtcgaagt tgtcggtcag
     2341 gatgatccgg tcgtccaccg cgatcgagta ggcgtccacg gtcgccgaga acccgcggga
     2401 ttcgaaggtg aatccgacgc tcccgttgac ggcagtctcc tcgtccagcc ggtcgatgcc
     2461 gaacgccttc gtgaccgtgc tcgtgttcga cgccaggagc gtccgcagcg aggcccccgc
     2521 cacgatgttg ttgaacacga ggttgtagtg gatctgcgcc agcgacggcg cgcggaatcc
     2581 cgtggagacc gatccgcgga ccgacagcgc cgggctgagc ctgtaccttc cggtcgcctt
     2641 gaaattcagg gtttccccga agtcgctgta gcgttcgtac cggaccgcac cgccgaccag
     2701 aagctcggaa gtcgcatcga gctctgcatc cgcatatacg ccgaagttgg atcggccgcg
     2761 gtcgaccacg ttggccgggc tgtacccggg gaagccctgg gagccgcccg gaagctggtc
     2821 gccgttcgag tcctcggccg gttcctggat ggccgggttc gtcaccgcca ccccgttctc
     2881 gtcgtagatc gcgtaggatg cctcctctcc ggcgaagatg gcgaaggttt ccgtgcggta
     2941 ttcgaggccg aacgccacgt tgagcccctc ggccacatcg gcgaagtacc gggagaggtc
     3001 caggttggtg gtgttcatgg agaggctgtg gccgcccgcg tcgaagtccg tgggagacgc
     3061 gtccccgagc gaggcgttgt tggtcccccg gatgaagtaa tggaagtcgt tcttgccgta
     3121 ggtgttggcg aagtccgccc gccatccggc ggccagggca tggcgcacgc cgacggtcgc
     3181 cgagacgtcc gtgatgttcg aggtgatcct gggggtgaac ccgtccgggt agaggctggg
     3241 caccgcccgg ttgtcgccgt cctcgaatcc gtcgcgggag aaggcgtacg catccgtgtc
     3301 acggaagctt cggccgccga acgcgtagac ctcggtatcg gcgccgagag gcagcgcgga
     3361 gttgacgagg aacgcgaacc cgtcgactgc ggccgtgccg tagcccttgc gccacgagaa
     3421 gccgggccgc agcgtccggt ccctcgtcag gaattccgtc gtgacgttcg cgaagccgcc
     3481 gttgtcgccg aggccgatgc cgtagttgag atccagcttc gtcgtggtcc catcgaagct
     3541 ccggtcctcc ccgtcgagcc ggttctcccc tttcacgttg tagatggtct ccccggtcgc
     3601 ctcggcatac ccgtccccga tcgccgtgct gtaggcgccg cgcgtgaggc tcgccgagag
     3661 gccgtccgtc cggtccttca gcacgatgtt gatgacgccc gcgatcgcgt cggagccgta
     3721 ctgcgcggat gcaccgtcgc gcaggatctc cacccgcttg atggcggacg ccgggatggc
     3781 gttcaggtcg gtgccggtgt ttccgcggcc ccgtgttccg aacacgttca cgagcgacga
     3841 ctggtgccgt cgcttgccgt tcacgagcac gagcgtctgg tccggcccga ggccccgcag
     3901 ggaggccggg tcgatgtggt cggcgccgtc ggacccagac tgcttcgtgg cgttgaacga
     3961 gggcgcggca acctgcagga tctggttcac ttcggccctg ccggtggtcg ccgccaggtc
     4021 cccgacgtcg atgacgtcca cggcgacggg cgagtccgtg accgagcggt tgacactcct
     4081 ggatcccacg acctggacct cgccgagcgt caggccctcc acgaggacca cgtcgatctg
     4141 cggccgaccg ccgactgcga tctcctgccg ctggaacccg acgtagctga agaccagggt
     4201 ggcgtcgcgc gagcccacct cgagccggta ccggccgtcc acgccggtcg tggtcccgtt
     4261 gaaggtgccc ttttcggcga tgttgacgcc gatcagcggc tcgttcgcgc cgtcggtgac
     4321 gattccggaa accgtgtgct gggcggatgc cgaccgcacg gcgaggacga ggagggcggc
     4381 aaggaggacg tgacgcatgg cctttccgac agtctggatg tgagggatga cccggggccg
     4441 tgtggggact gtactgtcgg ggcgtcggat aatcaagggg ccatagcgtg cgaacgaccg
     4501 ccgggatgct cctcgaatcc tgcgcgcaac cgcccgcacc cccctcaccc caccggcagc
     4561 gatacgacga acgtcgcgcc ccggcccggc tcgctctcga ccgtgagact gcccccgtgg
     4621 ccgctggtca cgatgtcgta cgagagcgac a
//
LOCUS       DBNHQZ010000092         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_26365, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000092 DBNHQZ010000000
VERSION     DBNHQZ010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_26365"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="AC16YU_02295"
     CDS             <1..1308
                     /locus_tag="AC16YU_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR6064078.1"
                     /translation="MEGTGGGARTGHVPVDDDNDGLYDEDGPDDLNGNGVYDQIRKHV
                     PGEGDYRISHDNPRIMEPVPAGEKGDWVMLGTEGIDNDGDGRVNEDGPGGYDPNRNWG
                     SDWQPNYVQGGAMDYPFQLPEARAVNDFLMSHSNIAGLQTYHNSGGMILRGPGAEWQG
                     NYPPEDIAFYDVLGQAGERMLPYYDYIVIWSGLYTVHGGSIDWSNDGLGIVSFSNELW
                     AGGQYFNSPKLQKQVEDPTSPISGQKGRYFFDEFVELGDQFVEWEAYDHPDYGPVEMG
                     GWKKTFGRVPPRFMNEELAHRNMAFTLYQADEMPLISLEKPTVERLGGDLYRVRVDIT
                     NSKVVPTITARAAANNVVDPDLLTLSGRSVEVVSAGWVRDKHRPGATDLVDQHDLSRI
                     MIRNGHPGHTTRTIEYLVRGSGEVTVRYASLKGGTGEVTVALR"
     gene            1338..2588
                     /locus_tag="AC16YU_02300"
     CDS             1338..2588
                     /locus_tag="AC16YU_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010596024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6064079.1"
                     /translation="MDRPVRLGLRENLAQFSLLVLVNAFVGAMVGMERSILPAIAEDE
                     FHLAARTAILSFIVVFGITKALTNYFAGRFSDRFGRKVVLVAGWLVAVPVPFLLMWAP
                     SWSWILAANALLGVSQGLTWSTTVIMKIDLAGPKMRGLAMGLNEFAGYFAVALSALAT
                     GWIAAEVGLRPEPFYLGVGFVVAGTALSVLAVRETRHHVTHESNLAGVTAGDRPSPRE
                     VIMRTSFTDRDLSSVSQAGLVNNLNDGMAWGLFPLVFAAASMTLTQIGTLAAIYPGVW
                     GLGQLFTGAWSDRIGRKGLIVAGMWTQGAGIVVIGLGATFAWFALGSVLLGLGTAMVY
                     PTLLAAIGDVAHPGWRASSVGVYRLWRDLGYAVGALLAGIVADAFGLSAATLTIAVLT
                     IASGVVVAVRMRETLRADEAGTPG"
     gene            complement(2709..3641)
                     /locus_tag="AC16YU_02305"
     CDS             complement(2709..3641)
                     /locus_tag="AC16YU_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6064080.1"
                     /translation="MKQTLLEALSDRVLVSDGAMGTQLMAAGLEHGGCGEIWNVTHPD
                     RVLAIQRRYVEAGADCLITNTFGGNGIMLGRRGHRDELSAANRAAARIAREAFGGRDG
                     FVLGDVGPIGGLMEPYGDLQVEDVRDGIRAQVRALVEAGVDAVIIETQTALEEAEIGV
                     LAAREFGAPCVIASMAYDLSPDGDACATMMGVDPETAARHLLDLGVDVVALNCGTGVD
                     MAVAARIVASYRESGARFTMAQPNAGLPELEGGRIVYRQDPDAYVRPIRSLLDAGVNI
                     VGGCCGTTPDHVRAIRREVDAWGGRNVRQKDATG"
     gene            complement(3638..3865)
                     /locus_tag="AC16YU_02310"
     CDS             complement(3638..3865)
                     /locus_tag="AC16YU_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013052503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HIR6064081.1"
                     /translation="MDVILESELTCPRCGRKSVETMPTNACVHFHPCPECGTVITPRK
                     GDCCVFCSYGSVPCPLEQLRNAATGAKTVSP"
     gene            complement(4065..>4364)
                     /locus_tag="AC16YU_02315"
     CDS             complement(4065..>4364)
                     /locus_tag="AC16YU_02315"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF020387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HIR6064082.1"
                     /translation="NDRARLRNHVYLSAECPEGQVTYVSIEALPGDWNALPYRSSSQR
                     FGDAWIDRQESLVLGVPSVIVPEEWNFLVNPAHPAFGGITVSEPRRAAFDPRLLR"
BASE COUNT          655 a         1514 c         1513 g          682 t
ORIGIN      
        1 atggagggca ccggcggcgg cgcgcgcacc gggcacgttc cggtcgacga cgacaacgac
       61 gggctctacg acgaggacgg ccccgacgac ctgaacggca acggggtcta cgaccagatc
      121 cgcaagcatg tcccgggcga gggcgactac cggatcagcc acgacaaccc gcggatcatg
      181 gagcccgtac ccgccggcga gaagggcgac tgggtgatgc tgggcaccga gggcatcgac
      241 aacgacgggg acggccgcgt gaacgaggac ggccccggcg gctacgaccc caaccggaac
      301 tggggatcgg actggcagcc caactacgtc cagggcggcg ccatggatta cccgttccag
      361 ctgcccgagg cgcgtgccgt gaacgacttc ctcatgagcc actccaacat cgcgggcctg
      421 cagacctacc acaactcggg aggcatgatc ctgcgcggcc cgggcgccga gtggcagggc
      481 aactatccgc ccgaggacat cgccttctac gacgtcctgg gccaggcggg cgagcgcatg
      541 ctcccgtact acgattacat cgtgatctgg agcggcctct acaccgtgca cggcggctcg
      601 atcgactggt cgaacgacgg gctaggcatc gtctccttct cgaacgagct gtgggcgggc
      661 gggcagtatt tcaacagtcc caagctgcag aagcaggtgg aggatcccac gagccccatt
      721 tcgggacaga agggccgcta cttcttcgac gagttcgtgg agctgggcga ccagttcgtc
      781 gagtgggagg cctacgacca ccccgactac gggcccgtcg agatgggcgg gtggaagaag
      841 accttcggcc gcgtgcctcc gcgcttcatg aacgaggagc tcgcgcaccg caacatggcc
      901 ttcacgctct accaggcgga cgagatgccg ctaatctcgc tcgagaagcc gacggtcgag
      961 cgcctgggcg gcgacctgta ccgcgtgcgg gtcgacatca cgaactcgaa ggtcgtcccg
     1021 acgatcactg cccgcgcggc cgcgaacaac gtcgtcgacc ccgatctcct gacgctttcg
     1081 ggccgcagcg tcgaggtggt ctcggcgggc tgggtgcgcg acaagcaccg ccccggcgcg
     1141 acggacctcg tcgaccagca cgacctctcg cggatcatga tccggaacgg gcacccgggc
     1201 cacacgacgc ggaccatcga gtacctggtg cgggggagcg gggaggtgac ggtgcggtac
     1261 gcgagtctga agggggggac gggggaggtg acggtcgcgc tgaggtgatt cgcgccgccc
     1321 acatcccggg cgcgccgatg gaccgccccg ttcgcctggg cctgcgcgag aacctcgcgc
     1381 agttctcgct gctcgtcctg gtcaacgcgt tcgtcggcgc catggtcggc atggagcgga
     1441 gcatcctgcc cgccatcgcc gaggacgagt tccacctcgc ggcgcgcacc gcgatcctgt
     1501 cgttcatcgt cgtcttcggc ataacgaagg cgctgacgaa ctacttcgcc gggcgcttct
     1561 ccgaccgctt cggtcgcaag gtcgtgctgg tcgcgggttg gctggtggcg gtgcccgtgc
     1621 cgttcctcct gatgtgggcc ccctcgtggt cgtggatcct cgcggccaac gcgttgctcg
     1681 gcgtcagcca gggcctgacc tggtcgacca cggtcatcat gaagatcgat ctcgcgggtc
     1741 cgaagatgcg cgggcttgcc atggggctca acgagttcgc cggctacttc gccgtcgcgc
     1801 tctctgcgct cgcgacgggg tggatcgcag ccgaggtcgg actgcgtccc gagccgttct
     1861 acctgggcgt gggcttcgtg gtcgcgggaa ccgcgctctc ggtcctggcg gtccgggaga
     1921 cgcgccatca cgtcacccac gagtcgaacc tcgcgggcgt cacggcgggg gaccgtccgt
     1981 cgccgcgcga ggtcatcatg cgcacgagct tcaccgaccg tgacctgtcg tcggtgagcc
     2041 aggcgggcct ggtcaacaac ctcaacgacg gcatggcctg ggggctcttc ccgctcgtct
     2101 tcgccgccgc gagcatgacg ctgacccaga tcgggacgct ggccgccatc taccccggcg
     2161 tctggggcct cggacagctt ttcacggggg cctggtccga tcgcatcggc cgtaagggcc
     2221 tgatcgtcgc cggaatgtgg acgcaggggg cgggcatcgt cgtcatcggg ctcggtgcga
     2281 cgttcgcctg gttcgccctg ggatccgtac tgctcgggct cgggacggcg atggtgtacc
     2341 ccacgctcct ggccgccatc ggcgacgtcg cccatccggg ctggcgcgcg tcttcggtcg
     2401 gagtctaccg cctgtggcgc gacctgggct acgccgtggg cgcgctcctg gccggcatcg
     2461 tggccgacgc cttcgggctc tcggccgcga ccctgacgat tgccgtgctc acgatcgcat
     2521 cgggcgtcgt cgtggccgtg cgtatgcgcg agacgctgcg cgcggacgaa gcgggcacgc
     2581 cggggtagcc ttccctccac gattcaacgg atcccggcgc gtcgcgggtc cgcgcgcggc
     2641 gcggccgatg acgggctggc cgaggccacg cgccgtggca ccatgaaggc cagggtgcct
     2701 ggcgccgatc agcccgtcgc gtccttctgc cggacgttcc gcccgcccca cgcatccacc
     2761 tcccgccgta tcgcgcgcac gtgatccggc gtcgtcccgc agcacccgcc gacgatgttc
     2821 acgccggcgt cgagcaggct ccggatcggg cggacgtacg cgtccgggtc ctggcgatag
     2881 acgatccgac cgccctcgag ctccggaagg ccggcgttcg gctgggccat cgtgaagcgg
     2941 gcgccgctct cgcggtacga cgcgacgatc cgcgcggcca cggccatgtc cacgcccgtg
     3001 ccgcagttga gggccacgac gtccacgccc aggtccagga ggtgccgggc ggccgtctcg
     3061 gggtcgacgc ccatcatggt cgcgcacgcg tccccgtccg gcgacaggtc gtacgccatg
     3121 gacgcgatga cgcacggcgc cccgaactcc cgggcggcca ggaccccgat ctcggcctcc
     3181 tcgagcgcgg tctgcgtctc gatgatgacg gcgtcgacgc cggcctcgac gagggcccgg
     3241 acctgcgcgc gaattccgtc ccgcacgtcc tcgacctgca ggtcgccgta cggctccatg
     3301 agccccccga tcggccccac gtcgccgagc acgaaaccgt cccggccgcc gaaggcctcg
     3361 cgcgcgatgc gggcggcggc gcggttggcg gcggaaagct cgtcccggtg gccgcgccgc
     3421 ccgagcatga tgccgttgcc cccgaaggtg ttcgtgatga ggcagtccgc gcccgcctcc
     3481 acgtagcggc gctggatggc gagcacccgg tcggggtggg tcacgttcca gatctccccg
     3541 catccgccgt gctccaggcc cgcggccatc agctgcgtgc ccatcgcgcc gtcggacacc
     3601 agcacgcggt cggagagggc ttcgaggagg gtctgcttca tggggagacg gtctttgcgc
     3661 ccgttgcggc gttgcggagc tgttccagag ggcagggcac ggagccgtat gagcagaata
     3721 cgcagcagtc ccccttccgg ggcgtgatca ccgtcccgca ctccgggcac gggtggaaat
     3781 ggacgcaggc gtttgtgggc atcgtctcga cggacttgcg gccacaccgg ggacaggtca
     3841 gttcactttc caggatgaca tccatcgaga tgcgggggga aaggcggggt ccgatgccgg
     3901 acgacgctca accgagggca tacgggcggg aatcccgccg gttcgcgcgg aggcccgcgc
     3961 ctaccgctgc cagagtccgt gcccgacgac ctgttgtcgc agtatggtcc gggtgtcgcg
     4021 cttcgtaatg cgagtcccga gttgtgctgg ccgtgatcgt cacgctatct gaggagtcgc
     4081 ggatcgaatg ccgcgcgcct gggctcgctc acggtgattc cgccaaatgc cggatgggcc
     4141 gggttcacga ggaaattcca ctcctccgga acgatcacgc tcggcactcc cagaacgagc
     4201 gattcctgcc tgtcgatcca cgcgtcgcca aatcgctgcg aactgctccg gtaaggcagc
     4261 gcgttccaat ccccgggaag agcctcgatg ctcacatagg tgacctgccc ctcggggcat
     4321 tccgcactca gatacacatg gttccggaga cgggcccggt catt
//
LOCUS       DBNHQZ010000093         5430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2870807, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000093 DBNHQZ010000000
VERSION     DBNHQZ010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2870807"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="AC16YU_02320"
     CDS             complement(<1..1277)
                     /locus_tag="AC16YU_02320"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR6064083.1"
                     /translation="MRRNASTRLLPILAVVAAVLSGCTPSFEGYTTRDGQIVDRATGE
                     PVHLVGFGLGAWLLPEGYMWGIRSLDRPRQFEAAIEDLIGPEDAAEFWRLYHDNYMTR
                     EDFRAMATMGANSIRPALLASKLMPPEQPAGGPYVFSDEGFRFLDSLVAWATGYRVGL
                     IWDMHGAPGAQNAENIADSDGEARLWTQPDVYWPRLNALWRRIAERYAGNPIIIGYDL
                     LNEPLLRRYEGIDEADLRRLYVELTATIREVDPHGIIFIEGDDWAQNFRALEPLDWDP
                     HLVVAFHSYPPTSSAEGLARWDSLRVRYGVPLWHGETGEVGPPYDRNREATAFLRSAN
                     VGWSWWTHKKLASQTQPWYCPRTAGFLRILDYWQGRAERPTREEARAWLFDQAEKVNT
                     RYCDFLPEMVASLDGLDPQAWLAVRGREAPAIYL"
     gene            complement(1380..2696)
                     /gene="nhaA"
                     /locus_tag="AC16YU_02325"
     CDS             complement(1380..2696)
                     /gene="nhaA"
                     /locus_tag="AC16YU_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6064084.1"
                     /translation="MGIQPNALSSNPVVRPFQAFFRTEAAGGILLLVCALVALVWANT
                     PWAASYFALWSTVVTAGFGSFEISKPLLLWINDGLMAIFFFVVGLEIKREVLMGELGT
                     PRKAALALVAALGGMVVPALLYAAVNAGGEGAAGWGIPMATDIAFALGVLALLGKRAP
                     LALKVFLTALAIVDDLGAVLVIAFFYTAQLKLGYLAFAAGVLAALMILNRTGVRRTAP
                     YVVLGLALWLAFLKSGVHATIAGVLLALTIPARRLLDDSAFLARGRELLDRFARDVKP
                     GEGLPTADQVDVLHALEDAAGRLDTPLHRMEHALHGWVAFGIMPVFALANAGVALGGG
                     VSLGSPVTLGVILGLFAGKQLGVTGFSWLAVRLGWAELPQGVSWRQLYGVSLLTGIGF
                     TMSLFIANLAFDNQALLDGAKVGILAASLIAGVAGWLVLSRVGARE"
     gene            3015..3320
                     /locus_tag="AC16YU_02330"
     CDS             3015..3320
                     /locus_tag="AC16YU_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064085.1"
                     /translation="MRNVLLVTVLVLTGLLVIAFLLYLADGTPLGVLPVAGIMWIVLA
                     PIPAFVASRNGRSFFGYLLLALAISPVLGLAVVAALGKPAYLRNRRRIRSHEFVMPL"
     gene            3382..3828
                     /locus_tag="AC16YU_02335"
     CDS             3382..3828
                     /locus_tag="AC16YU_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR6064086.1"
                     /translation="MKRVHGDLLALLQEGTFDVIVHGCNCMASMGGGLAAQVAATYPE
                     VEEADREYDLHRPGRLKAGTILPVDVEDGTVVNAYTQVMGGPAAEPVLIKQALWKVRA
                     LYGHEDVRIGIPKIGCGIGGLDWHEVEPIVEEELGGLDVTLVEYGA"
     gene            3951..>5430
                     /locus_tag="AC16YU_02340"
     CDS             3951..>5430
                     /locus_tag="AC16YU_02340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6064087.1"
                     /translation="MKRLLLLVLAAVTVLPAAAQKRPMTTDDGLRMHDLSTALMTPDG
                     TGVLYGVSDLDWDENKRETTYFRIPADSGEAYPYLGKEGGSDLKYSPDGRFLSFKRTV
                     DKKAQVFVLRTAGGEARQLTKHDEAVGSYAWLPGSDALVFVAEWPRSKDEQKEFEKGD
                     DGIFVDEGPNGQTAARWQSLYRVAVPEGKIRRLTRDSLRIGSPAVAPDGRSVAVAVRR
                     ENRRNDQWRSEIYRFTFADSSLVRLTRNGAPEGSLEWAPDSRRLAFTAPDTADWDLRN
                     DKIWILDTSSGGISMVSGGFTGNIGTYAFTPDGGYILFNGLHRTNRNLYRLALDTGQV
                     TALTDVVGSLDVQDFSKDRTRMVYQYSSFSVAPDLYTSPTTALAPRRLTDLNPIVRDS
                     LILARAETVRWNSSDGLEIEGLMLTPPGASAPMPLLLHIHGGPAGVFTNRFSFREHVW
                     AGLGYLQLMPNVRGSSGYDDALLRGNMRDIGGGDFQDLMTGVD"
BASE COUNT          877 a         1835 c         1917 g          801 t
ORIGIN      
        1 aggtagatgg ccggcgcctc gcgaccgcgc acggcgagcc aggcctgcgg gtccagcccg
       61 tcgagcgacg cgaccatctc ggggaggaaa tcgcagtacc gcgtgttcac cttctcggcc
      121 tggtcgaaga gccacgcgcg ggcctcctcg cgtgtcgggc gctcggcccg gccctgccag
      181 tagtccagga tacgcaggaa gccggcggtt cgggggcagt accagggctg cgtctggctc
      241 gcgagcttct tgtgcgtcca ccagctccag ccgacgttcg ccgagcgcag gaacgcggtc
      301 gcctcgcggt tgcggtcgta gggcgggccg acctcgcccg tctcgccgtg ccagagcggc
      361 acgccatagc gcacgcgaag gctgtcccac cgggcgagcc cttcggcgga agaggtgggc
      421 gggtaggaat ggaaggccac gaccaggtgc ggatcccagt cgaggggctc gagggcgcgg
      481 aagttctgcg cccagtcgtc gccctcgatg aagatgatgc cgtgcgggtc gacctcccgg
      541 atggtggcgg tgagctccac gtacagccgc cgcaggtccg cctcgtcgat gccttcgtac
      601 cggcgaagga gcggctcgtt caggagatcg tagccgatga tgatcgggtt gcccgcgtat
      661 cgctcggcga tccgccgcca gagggcgttg agcctcggcc agtacacgtc gggctgggtc
      721 cagagacggg cctcgccgtc cgagtccgcg atgttctcgg cgttctgcgc cccgggtgcg
      781 ccgtgcatgt cccagatcag gcccacacgg tagcccgtgg cccaggcgac gagcgagtcc
      841 aggaagcgga aaccctcatc cgagaacacg tacggtcccc cggccggctg ctcgggcggc
      901 atgagcttcg aggccaggag cgccggccgg atggagttcg cgcccatggt cgccatcgcc
      961 cggaagtctt cccgggtcat gtaattgtcg tggtacaggc gccagaattc ggccgcgtcc
     1021 tcggggccga tcagatcctc gatggccgcc tcgaactgcc gtgggcgatc cagcgagcgg
     1081 atgccccaca tgtagccctc ggggaggagc caggcgccga ggccgaaacc caccaggtgc
     1141 acgggctctc ccgtggcccg gtccacgatc tggccgtcgc gggtcgtgta gccctcgaac
     1201 gagggtgtgc agcccgagag gactgcagcg acgacggcga ggatgggcag aagtcgggtg
     1261 gaggcgttgc ggcgcatggc gtccctacac ttgtcaggct gccggaaagg tagcggctcg
     1321 caagggcccg ggccgtgaac aaactgagag tccggcggca ggcttgccgc agcggcgccc
     1381 tactcccgcg ccccgacgcg cgacagcacc agccaccccg ccacccccgc gatcagcgac
     1441 gccgccagga tgccgacctt cgccccgtcg agcagggctt ggttgtcgaa ggcgaggttc
     1501 gcgatgaaga gcgacatcgt gaatccgatg cccgtgagaa ggctcacgcc gtagagctgt
     1561 cgccaggaga cgccctgcgg gagctccgcc caccccaacc gcacggcgag ccacgagaag
     1621 cccgtgaccc cgagctgctt acccgcgaag agcccgagga tcacgcccag cgtgacgggg
     1681 cttccgagcg agactccgcc gccgagcgcc acgcctgcgt tcgcgagggc aaagacgggc
     1741 atgatgccga aggccaccca gccgtgcagc gcgtgctcca tgcggtgcag cggggtgtcc
     1801 aggcgcccgg cggcgtcctc gagggcgtgc agcacgtcta cctggtccgc cgtcggaagc
     1861 ccctcgcccg gctttacgtc gcgcgcgaaa cggtccagga gctcgcggcc gcgcgcgagg
     1921 aaggccgagt cgtcgagcag gcggcgggcc gggatcgtca gggcgagcag gacgccggcg
     1981 atcgtggcgt gcacgccgga cttcaggaag gcgagccaga gcgccaggcc cagcacgacg
     2041 tacggggccg tgcggcgaac gcccgtccgg ttcaggatca tgagcgcggc cagcacgcct
     2101 gccgcgaacg cgaggtagcc cagcttgagc tgtgccgtgt agaagaaggc gatgacgagg
     2161 acggccccca ggtcgtcgac gatggcgagc gccgtgagga agaccttcag cgcgagcggg
     2221 gcgcgcttgc cgagcagggc caggacgccg agcgcgaacg cgatgtccgt cgccatgggg
     2281 atgccccacc cggccgcccc ttcgccgccc gcgttcaccg cagcgtacag cagcgcggga
     2341 accaccatgc cgccgagggc cgccaccagg gcgagggccg ccttacgcgg agtgcccagc
     2401 tcgcccatga gcacctcgcg cttgatctcg agccccacca cgaagaaaaa gatggccatg
     2461 aggccgtcat tgatccagag caggagcggc ttcgagatct cgaagctccc gaatcctgcc
     2521 gtgacgacgg tgctccagag cgcgaagtac gaggcggccc acggcgtgtt ggcccacacc
     2581 agcgccacga gtgcgcagac gaggagcagg atgcccccgg ccgcctccgt gcggaagaac
     2641 gcctggaagg ggcggacgac aggattcgag gacagggcgt tcggttggat tcccatggtt
     2701 cggcggatgc gggggacggt cctggaaccc cgtctatagt cgttgtaacg acgagttccg
     2761 gggttccaaa cgttttcgac ggttctgaac ggaagcgggg tccgattcgc ggtcgcgacc
     2821 gtccggcggc ggcccgggcg tccgtcccgc gggggccggt cctgggcact gaaatgcgcc
     2881 tccggcctgt cgctccgcaa cgtaccgctt cgtttccatt cttgccccct gacctggagg
     2941 agcagacgat gggcactgct tcacaccgaa accgggcgtc cttcaaggga cgccgaaccg
     3001 aatcgacatc cggaatgcgc aatgtgctgc tcgttaccgt gctggtcctg accggactcc
     3061 tggtgattgc gttcctcctg tacctggccg acggaacacc gctcggcgtc ctgcccgtgg
     3121 cgggaatcat gtggatcgtg ctggccccga taccggcgtt cgtcgcctcg cgcaacggcc
     3181 ggtccttctt cggctacctg ctgctcgcgc tcgccatttc cccggtcctc ggcctcgcgg
     3241 tcgtggcggc gctcggcaag ccggcgtacc tgcgcaatcg gcgccgcatc cggagccacg
     3301 agttcgtcat gccgctgtga cggccttgac gaaagcagct tttcgaacgt acccttccaa
     3361 ggggaaggaa ccggtggagc catgaaaagg gttcatggag atctcctggc gctccttcag
     3421 gagggcacct tcgacgtgat cgtgcacggg tgcaactgca tggcgtcgat gggcggagga
     3481 ctcgccgcgc aggtcgcggc gacgtatccg gaggtcgagg aggccgaccg cgagtatgac
     3541 ctgcaccggc ccggacggct gaaggccggc acgatcctgc ccgtcgatgt cgaggacggg
     3601 accgtggtca acgcctacac gcaggtcatg ggcggcccgg cagccgagcc cgtgctcatc
     3661 aagcaggccc tgtggaaagt gcgtgcgctc tacgggcacg aggacgtgcg catcggcatc
     3721 ccgaagatcg gctgcgggat cggggggctc gactggcatg aagtggagcc catcgtggag
     3781 gaggagctgg gcggcctgga cgtcacgctg gtcgaatacg gcgcctgacg ggagcgggga
     3841 atggcggcgc ggtgcacggg gtttcccgga tcgcgcgcgc tcgatttccc ggtatgcgct
     3901 gccgggcggg tgggctacat tcgcggcgtc accatcccga tgccaccgcg atgaagcgtc
     3961 tgctgttgct cgtgctcgcc gccgtcaccg tcctgccggc tgcggcccag aagcgcccga
     4021 tgaccacgga cgacgggctg cgcatgcacg acctctcgac cgccctcatg acgcccgacg
     4081 gcaccggcgt cctgtacggc gtctcggacc tggactggga cgaaaacaag cgcgagacca
     4141 cgtacttccg gatcccggcc gattccggcg aggcctatcc gtacctgggc aaggaggggg
     4201 gatcggacct gaagtactcg cccgacggcc gcttcctctc cttcaagcgg acggttgaca
     4261 agaaggcgca ggtcttcgtg ctccgcaccg cgggcgggga ggcgcggcag ctgacgaagc
     4321 acgacgaggc ggtgggatcg tacgcgtggc tgcccgggtc ggacgcgctc gtgttcgtgg
     4381 ccgagtggcc gcgctcgaag gacgagcaga aggagttcga gaagggagac gacggcatct
     4441 tcgtggacga gggcccgaac ggccagacgg ccgcccgctg gcagagcctg taccgcgtcg
     4501 ccgtgcccga gggaaagatc cgtcgactga cccgggattc gctccggatc ggctctcccg
     4561 ccgtggcgcc ggacggccgc agcgttgccg tcgccgtgcg gcgcgagaac cgtcgcaacg
     4621 accagtggag gtccgagatc taccggttca cgttcgcgga ctcgtcgctc gtgcgcctga
     4681 cgcgcaacgg cgcgccggaa ggctcgctcg aatgggcccc ggattcgcgc cgcctggcgt
     4741 tcaccgcccc ggacacggcc gactgggacc tgcgcaatga caagatctgg atcctggaca
     4801 cgtcttcggg cgggatcagc atggtgtcgg ggggcttcac gggcaacatc ggcacgtacg
     4861 ccttcactcc ggacggcggc tacatcctct tcaacgggct ccaccggacg aaccggaacc
     4921 tgtaccggct cgcgctcgac accggccagg tgaccgcgct gaccgacgtc gtgggcagcc
     4981 tggacgtgca ggatttctcg aaggaccgga cgcgcatggt ctaccagtac tcgtccttct
     5041 ccgtggcgcc cgacctgtac acctcgccga ccacggctct cgcgccccgg cgcctgaccg
     5101 acctgaaccc gatcgtccgg gactcgctga tcctggcccg ggccgagacc gtgcgctgga
     5161 actcgtcgga cggcctggag atcgaggggc tcatgctcac gccgcccggt gcgtcggcac
     5221 ccatgccgct tctcctgcac attcacggcg gtccggcggg cgtcttcacc aaccggttca
     5281 gcttccggga gcacgtctgg gcgggtctcg ggtacctgca gctgatgccg aacgtgcggg
     5341 gatcgagcgg ctacgacgac gccctgctcc ggggcaacat gcgcgacatc ggcggcgggg
     5401 acttccagga cctgatgacc ggcgtggacc
//
LOCUS       DBNHQZ010000094         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_838867, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000094 DBNHQZ010000000
VERSION     DBNHQZ010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_838867"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="AC16YU_02345"
     CDS             complement(<1..1746)
                     /locus_tag="AC16YU_02345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6064088.1"
                     /translation="MFPIQMDPEAFPAHTRAIFGLEDGDRPAWLPSAARPAAVRLVNC
                     SRRDFLRTGAAAAGSVFVLGLVPGCATPEELEDLGPKAPPSGLMGEAQFQPNVFVALN
                     SQGDVFLVNSRAEMGQGTKTGMTATLADEMDADWDRVHVVQADGHPKFGPQSTGGSQS
                     VRLFFQDWRKAGATARAMLVSAAALTWEVDEAECETAPHLVRHVPTGRELSYGALASA
                     ASTLPVPEAPALKARDEWRYIGKAMHGVDNHAIVTGAAQFGADVRVPDLKFAVIARSP
                     VLGGRLVSFDDSAARAVPGVLAVLEVPAFTGAPGTQALGGVAVVAENTWAALKGRDAL
                     AVSWDPGPNAVFDSGTYREALLDTVTRPGRVDREQGDVDAALAAADRVVEATYYTAML
                     EHAPMEPPATLAWVKTDGTCEAWCSTQDPQSARNLMAEALGVDAEQVTLHMTLLGGAF
                     GRKGLPDFAPEAALVSKQYGGPVLLTWSREDAMRHGYYHPPAAQHFKAAVDADGRVTG
                     WLHRTAFPAIGATFTEGAEYGGIQGARETMWAIPNLRIENGPAKAHVRIGWLRSVMAV
                     HHGFGIGAFVGELAHA"
     gene            complement(1749..2213)
                     /locus_tag="AC16YU_02350"
     CDS             complement(1749..2213)
                     /locus_tag="AC16YU_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6064089.1"
                     /translation="MATFTLTVNGRERTVEAEPDMPLLWVLRDLLGLTGTKYGCGQAL
                     CGACTVHLDGRAVRSCETEIQDVGGAVVVTIEGLSEEGDHPLQVAWKELDVAQCGYCQ
                     PGHIMEAAAWLASTPRPTAEDIDREQSDVLCRCGTYERIRAAIVRAAEIQGR"
     gene            2378..>2989
                     /locus_tag="AC16YU_02355"
     CDS             2378..>2989
                     /locus_tag="AC16YU_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6064090.1"
                     /translation="MSDSVETLVVGGGLAGWCAARVLAGRGSVSLATMEHAADAHASS
                     AAALAGIANPLMGRRGHPVWRADQAMASLERLIEDTGPDLVHRLPGIIHPAVSSWQVD
                     DFRRSAADRPDIATWWGAGLVAERAPWLTAPRGALVIQRGCALSVPDLLEAVEADASD
                     RGVVTRRGWRLTGWREEQEGVTADFETAAGPASLHAGRLVLALG"
BASE COUNT          413 a         1083 c         1063 g          430 t
ORIGIN      
        1 cgcgtgggcc agctctccca cgaacgcgcc gatgccgaac ccgtggtgca cggccatcac
       61 ggagcggagc cagccgatgc gcacgtgcgc cttcgccggg ccgttctcga tgcggaggtt
      121 cggaatcgcc cacatcgtct cgcgagcgcc ctggatgccc ccgtactcgg ctccctcggt
      181 gaacgtcgcc ccgatcgcgg ggaacgccgt ccggtgcagc cagccggtca cgcggccgtc
      241 cgcatccacg gccgccttga agtgctgcgc cgcgggtgga tggtaataac cgtgccgcat
      301 ggcgtcctcc cggctccagg tgagcaggac cggcccgccg tactgcttcg agacgagcgc
      361 cgcttccggc gcgaagtccg gaaggccctt ccgtccgaac gcgccgccca ggagcgtcat
      421 gtgcagggtg acctgctccg cgtcgactcc cagcgcctcg gccatgaggt tgcgtgccga
      481 ctgcgggtcc tgggtcgagc accaggcctc gcaggtcccg tcggtcttca cccaggcgag
      541 ggtcgcgggg ggctccatcg gggcatgctc gagcatggcc gtgtagtagg tcgcctcgac
      601 cacgcggtcg gccgcggcga gcgcagcgtc cacgtcgccc tgctcccggt ccaccctgcc
      661 cgggcgggtc accgtatcca ggagcgcctc gcggtacgta cccgaatcga acacggcatt
      721 cggccccgga tcccacgaca cggcgagggc atcgcgcccc ttgagggccg cccacgtgtt
      781 ctcggcgacg accgccactc cgcccagggc ctgggtaccc ggcgctcccg tgaaggcagg
      841 gacctcgagc accgccagca cgccgggcac ggcccgggcc gccgagtcgt cgaacgacac
      901 gagccggccg cccagcacag gcgagcgcgc gatgacggcg aatttcaggt ccggcacgcg
      961 gacgtcggcg ccgaactgcg ccgccccggt cacgatggcg tggttgtcca cgccgtgcat
     1021 cgccttgccg atgtagcgcc actcgtcgcg ggccttcaga gccggcgcct ccggaacggg
     1081 cagcgtcgag gcggcgctcg ccagcgctcc gtacgagagc tcgcgcccgg tcggcacgtg
     1141 gcgaaccagg tggggagccg tctcgcattc ggcctcgtcc acctcccagg tcagggcagc
     1201 tgccgacacg agcatcgccc gcgcggtggc gcccgccttc ctccagtcct ggaagaagag
     1261 acgaacggac tgcgagccgc cggtcgactg cgggccgaat ttcgggtgac cgtccgcctg
     1321 gaccacgtgc acgcggtccc agtcggcatc catttcgtcg gccagcgtgg ccgtcatgcc
     1381 ggtcttcgtc ccctgcccca tctcggcgcg ggaattgacc aggaagacgt cgccctgcga
     1441 gttgagcgcg acgaacacgt tcggctggaa ctgcgcctcc cccatgagcc cggacggagg
     1501 cgccttgggg ccgagatcct ccagctcttc gggggtcgcg caccccggca cgaggccgag
     1561 gacgaagacc gagcccgcgg ccgcggcccc cgtccggaga aaatcgcgcc gggagcagtt
     1621 gacgaggcgc acggccgcag gacgcgcggc ggagggaagc caggccggcc ggtcaccgtc
     1681 ctcgagtccg aagatcgccc gggtatgggc cgggaaggcc tcggggtcca tctggatggg
     1741 aaacatggct acctcccctg gatttcggcg gcccgcacga tggccgcccg gatgcgctcg
     1801 tacgtgccgc agcggcacag cacgtcagac tgttcgcgat cgatgtcctc cgccgtgggc
     1861 cggggcgtgg acgcgagcca ggcggccgct tccatgatgt gcccgggctg gcagtagccg
     1921 cactgcgcga cgtcgagctc cttccaggcg acctgcagcg gatggtcgcc ctcctcgctg
     1981 agcccctcga tcgtcaccac gacggcgccg cctacgtcct ggatttccgt ctcgcaggaa
     2041 cggacggccc ggccgtccag gtgcacggtg caggccccgc aaagggcctg tccgcagccg
     2101 tatttcgtgc cggtcaggcc caggaggtct cgcaggaccc acaggagcgg catgtcgggc
     2161 tcggcctcga cggtgcgctc ccggccgttt acggtcaggg tgaaagttgc catggtaccg
     2221 ggatggtgat tggacgagca ctgcagggta agcaccccgc cggacggaat cctgtcatga
     2281 ccggcgcgat catttgcggg tggaaccctg acgcatcgcc gaacccgtgt cctatcttcc
     2341 gccggtttca ttcccgcgct gccgtccagg ccaccgcatg tccgactccg tggaaacgct
     2401 cgtagtgggc ggcggcctgg ccggctggtg cgccgcgcgc gtgctcgccg gccgcggatc
     2461 cgtctccctg gccacgatgg agcacgcggc ggacgcgcac gcctcgtccg ccgcggcgct
     2521 cgcgggcatc gccaacccgc tgatgggacg gcgcggacac cccgtgtgga gggcggacca
     2581 ggcgatggcc tcgctcgagc ggctcatcga ggacacgggg cccgacctgg tgcaccgact
     2641 gccggggatc attcaccccg ccgtgtcgag ctggcaggtg gacgattttc gccggtcggc
     2701 ggcagaccgt ccggacatcg ccacgtggtg gggcgccggc ctcgtcgcgg agcgtgcccc
     2761 gtggcttacg gcgccgcggg gagcgctcgt gatccagcgc ggatgtgcgc tctcggttcc
     2821 ggacctgctg gaggccgtcg aggccgacgc gtcggatcgg ggcgtggtca cgaggcgcgg
     2881 ctggcgcctc accggctgga gagaggagca ggagggcgtc acggccgatt tcgagacggc
     2941 cgccggcccg gcgtcgctcc acgccgggcg gctggttctg gcgctgggc
//
LOCUS       DBNHQZ010000095         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3141512, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000095 DBNHQZ010000000
VERSION     DBNHQZ010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3141512"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="AC16YU_02360"
     CDS             <1..738
                     /locus_tag="AC16YU_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6064091.1"
                     /translation="VTTVDGRLRPDLIIRLPGDRIIVVDAKVPLEAYLDSVNMDDEVA
                     RKAAMQRHVSQIREHVKKLGAKAYFDQFPTTPDMAVMFLPGESFYYAALQEDPSLLEY
                     GVGNNVVIATPMTLIALLRAVSYGWRQESMAENAREIAALGRELYDRMIVLAEHFARV
                     GSGLGTAVDNYNRAVGSFEGRVLVQARRFRDLGAGSSREIQEMGPVDKSPRSIDIPEF
                     RASRALTAGGDADNRPALPPADAVDEP"
     gene            844..1758
                     /locus_tag="AC16YU_02365"
     CDS             844..1758
                     /locus_tag="AC16YU_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6064092.1"
                     /translation="MTPTPDAEGRPALSAVLLAFAALYLVWGSTYLAIRVAVETIPPF
                     LMLGARFAFTGGVLYAWMRVRGAARPTASEWRTAGWTGGLMLMGGTGLVGVAEQYIPS
                     GLTALLLSTTPLWMVLLAWLWKGEKRPHGVVFAGIALGLVGVYFLFDTESLSAEAGAG
                     PMPGLLVLLAAVSWSVASIAAREGGLPRNPFLASAMQMISGGVLLFVAATVTGEWSAF
                     RPEAVTLHSWLSLGYLVVFGSFVAFSAYVWLLRQTTPARVSTYAFVNPGVAVVLGWLI
                     LAEPFTARIAVALAFLTVSVVLIVRYGR"
     gene            complement(1805..2212)
                     /locus_tag="AC16YU_02370"
     CDS             complement(1805..2212)
                     /locus_tag="AC16YU_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064093.1"
                     /translation="MIRTSTGRTATVAITVLVVAFLSAPPAVAQGFDPERARQRMTEQ
                     VGETVKALALEGETADTVRSILMARVEKRMELFASMRPGEGRGPEGMREAMAKLDTEA
                     EGRLAGVLSKEQIDAWKAHEEALRSRMRRGPRG"
     gene            complement(2319..2792)
                     /locus_tag="AC16YU_02375"
     CDS             complement(2319..2792)
                     /locus_tag="AC16YU_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064094.1"
                     /translation="MLIPVPAQTPAPAPSDEPRDMAVALRLLSGKDCERDASLAHTML
                     VRLSESPDEVVSSEATRVLKAGVSNRWFVDRAPHHYELERIAEKTLARRRAAPDSRRS
                     ALAIGIGLTALLGGLVAYLAAGDSPGDGLGGQMLIAAVVIALLVAMVLYRFAARS"
     gene            2931..>3279
                     /locus_tag="AC16YU_02380"
     CDS             2931..>3279
                     /locus_tag="AC16YU_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064095.1"
                     /translation="MSDSLATFLQEPGVRTARKLLVAPSQQAGVSVRTALARAGIRIA
                     GLEVSTPLGLARRIVDSADAGTGGDLLGPSTLAVQVACILHEMEEREGRPLMRSAGTI
                     ARSIAEARSAGLDV"
BASE COUNT          421 a         1127 c         1193 g          538 t
ORIGIN      
        1 gtgacgaccg tcgacgggcg gctgcggccc gacctgatca tccggctgcc gggcgaccgg
       61 atcatcgtgg tcgatgcgaa ggtgccgctc gaggcctacc tggactcggt gaacatggac
      121 gacgaggtgg cccgcaaggc ggcgatgcag cgccacgtgt cgcagatccg cgaacacgtg
      181 aagaagctgg gtgccaaggc ctacttcgac cagtttccga cgaccccgga catggcggtc
      241 atgttcctgc ccggcgagtc gttctattac gcggcgctgc aggaggaccc gtcgcttctc
      301 gaatacggcg tgggcaacaa cgtggtgatc gcgacaccga tgacgctcat cgcgctcctt
      361 cgcgcggtct cctacggctg gcggcaggag tctatggccg agaacgcgcg ggagatcgcc
      421 gccctcgggc gcgagctcta cgatcggatg atcgtgctcg ccgaacattt tgcccgggtc
      481 ggatcgggcc tgggcacggc cgtggacaac tacaaccggg ccgtgggctc gttcgagggc
      541 cgtgtgctcg tgcaggcgcg gcgcttccgg gacctgggcg cgggatcgag ccgcgagatc
      601 caggagatgg gaccggtcga caagtcgccg cgctcgatcg acatcccgga gttccgcgcg
      661 tcacgcgccc tgaccgccgg cggtgacgcg gacaatcgtc cggcgctacc gccggccgac
      721 gccgtggacg agccgtagcc ggcagtccgg cgagcggcgc ccgcgtggtc gcggtccgct
      781 ggcccgcgcc ccggagcata ggacggggag cggcgttcgg gagggattcc cgtcgccacg
      841 cccgtgaccc cgaccccaga cgccgagggc cggcccgcgc tctcggctgt cctcctcgcg
      901 ttcgccgcgc tctacctggt gtggggctcg acgtacctcg cgatccgggt cgcggtcgag
      961 accatcccgc ccttcctgat gctcggcgcg cggttcgcgt tcacgggcgg cgtgctatac
     1021 gcctggatgc gggtccgcgg cgcggcgcgg cccacggcct ccgagtggcg gaccgccggg
     1081 tggacgggcg ggctcatgct catggggggc acggggctcg tgggggtcgc cgagcagtac
     1141 atcccgtccg ggctcacggc cctgctcctc tcgaccacgc cgctctggat ggtgctgctc
     1201 gcctggctct ggaagggaga aaagcgcccg cacggcgtgg tattcgcggg catcgcgctg
     1261 ggcctcgtgg gcgtgtactt cctgttcgac acggaatcgc tgagcgccga ggcgggcgcc
     1321 ggtcccatgc ccggactgct cgtgcttctc gccgcggtct cctggtcggt agcgtcgatc
     1381 gccgcgcgcg agggcgggct gccgcgcaat cccttcctcg cgtccgccat gcagatgatc
     1441 tcgggcgggg ttctgctctt cgtcgccgcg accgtgacgg gcgagtggtc ggcgttccgg
     1501 ccggaggccg tcacgctgca ctcgtggctg tcgctcggat acctggtcgt gttcgggtcg
     1561 ttcgtggcat tctctgcgta cgtctggctg ctgcggcaga cgacgcccgc gcgggtgtcg
     1621 acctacgcct tcgtgaaccc cggcgtggcc gtggtgctgg gctggctgat cctggcggag
     1681 cccttcaccg cccgtattgc cgtggccctg gcgtttctga ccgtttcggt ggtcctgatc
     1741 gtgcggtacg ggcgctgagc gccaggatcg acgggggccg agccgccgtc tcgcccgacc
     1801 ggggtcagcc gcgcggtccg cggcgcatcc gcgagcggag cgcttcctcg tgcgccttcc
     1861 acgcgtcgat ctgctccttg ctcagcacgc cggcgagccg gccctcggct tccgtgtcga
     1921 gcttcgccat ggcctcccgc atgccttcgg gtccgcgccc ttcgccgggg cgcatcgagg
     1981 cgaacagctc catgcgcttc tccacgcggg ccatgagaat ggagcggacc gtgtcggccg
     2041 tctcgccctc gagcgccagg gccttgacgg tctcacccac ctgctcggtc atgcgctggc
     2101 gggcccgctc cgggtcgaat ccctgggcga ccgccggggg cgcggagagg aaggcgacga
     2161 cgaggacggt gatggcgacg gtcgccgttc ggccggtcga cgtgcgaatc atggcggggc
     2221 tccttctgtt gggtggatga aggtctaccg gaacggggag ccgcggttcg gaccgcgaca
     2281 gaatccgcac aactgcgacc cgttcgtgac gccggtcctc aggaccgggc ggcgaaccgg
     2341 tagagcacca tcgcgaccag cagggcgatg acgaccgccg cgatcaacat ctgcccgccg
     2401 agcccgtcgc ccggcgaatc ccccgccgcc aggtacgcca ccaggccacc gaggagcgcc
     2461 gtgaggccga tcccgatcgc cagggcggag cggcgcgaat cgggcgcggc ccgccgccgg
     2521 gcgagcgtct tctcggcgat ccgctccagc tcgtagtggt gcggcgcgcg gtcgacgaac
     2581 caccggttcg agacgccggc cttcaggacg cgcgtcgctt cgctgctgac gacctcgtcg
     2641 gggctttcgg acagccggac cagcatcgtg tgggcgagcg acgcgtcgcg ctcgcagtcc
     2701 ttgccgctga gcagccggag cgcgaccgcc atatcccgcg gctcgtcgga gggggccggg
     2761 gccggagtct gcgcgggaac gggtatgagc acggtcgtcg ccgggaggtg cctctcgtgt
     2821 cacgatacga aatccgtccc tgccatttgc cctgcgcctg gtgtaggagg ggtatgaaat
     2881 gttgatgtca tcctcgatat gtaccattca tcgtcgatct tcggattccc ttgagcgact
     2941 cgcttgccac atttctccag gagccgggcg tgcgcacggc ccggaagctc ctcgtcgcgc
     3001 cttcgcagca ggccggcgtc tcggtgagaa ctgcgctcgc gcgggcggga atccgcattg
     3061 ccggcctcga ggtgtcgact ccgctcggtc tggccaggcg gatcgtggac tcggccgacg
     3121 ccgggaccgg cggcgacctc ctggggccgt ccacgctggc ggtacaggtg gcctgcatcc
     3181 tccacgagat ggaggagcgg gaggggcggc cgctcatgcg gtcggcagga acgatcgcga
     3241 ggtcgatcgc ggaagcccgc tcggcgggcc tcgacgtgg
//
LOCUS       DBNHQZ010000096         3655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3413471, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000096 DBNHQZ010000000
VERSION     DBNHQZ010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3413471"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="AC16YU_02385"
     CDS             complement(<1..355)
                     /locus_tag="AC16YU_02385"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6064096.1"
                     /translation="MLIPIGDDDRELYRPSLVTKALLLVNAAVFFLLQGANSENAFTY
                     GWAAIPYELTTGIDLTGIHQILVDGTVANIPHAPGPTPIWLTALSAMFMHGGFGHLFG
                     NLLYLWIFGDNVEHRF"
     gene            complement(366..671)
                     /locus_tag="AC16YU_02390"
     CDS             complement(366..671)
                     /locus_tag="AC16YU_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HIR6064097.1"
                     /translation="MSGKATIAERLGMVAYRNQGRTDARPHILVDTAICNSTCPHRCT
                     TYVCPANCYTVDEAGQVRFQFEDCIECGTCLYACDQGAVRWSFPDPEAGRGVNWNLG"
     gene            complement(668..1990)
                     /locus_tag="AC16YU_02395"
     CDS             complement(668..1990)
                     /locus_tag="AC16YU_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6064098.1"
                     /translation="MDERFDCIIVGGGIAGLSAAMTLARAGARFLLIERGEFCGAKNV
                     SGGVLWGRDLERLVPGYSGQEAGWDRYVAHRRLTFMDEGSAFSVDFKSSHFDEAPYSG
                     ITVLRARFDAWLAERVEEAIAESGRADESFVATNVLVDEILLEDGRAVGIRAGGERFG
                     ADCVILAEGVNNLLTRQVGLEADYVPADHVAVGVKEVLHLDRAVLEDRFQLHGRSGLT
                     NEFVGACTAGVEGGGFLYTNLESISLGLVLGMADLREKGLKPYDVLNTFKAHPAVADM
                     IRGAEPVEYSAHVVSTGDLRGMPREIFGDGVLVAGEAAHLLLNAGRAIQGMDYAMRSG
                     ILAAEGVLSAREAGDFSAGMLSSYRRALEGSYVLRDMRSFQDAVRLLHDPAMHTSVPS
                     VVCDFGRQFFTIDNEPTARASTMFRTAVKRHASYWELVKLGVRGARAL"
     gene            complement(1990..2970)
                     /locus_tag="AC16YU_02400"
     CDS             complement(1990..2970)
                     /locus_tag="AC16YU_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6064099.1"
                     /translation="MSSILVFVSTVEGRIRRSSLEVLSHARRQASVAGLAVDALVVGP
                     DPDAAVDGVAAYGPRRIYTVKDPVFCSPLNAPMIQALAAGCRVSEARVLAMASTEAVK
                     DVLGALSVRLAAPALPDVSDFTVLAEGVEALRPVMASKQMARVRAEGQPVLVSVRSGS
                     FEAEEAPSIPEVVPVPFTFDEGSLGQTLREIVRGAGGTVDLSEARVVVAAGRGVRDEE
                     GKRLVEELAGLFGGAIGASRAVVESGLFPATAQIGQTGKVVSPDLYIAVGVSGAIQHV
                     AGMINSRVIVAINKDAEAPIFRVANYGLVGDLYRILPPLITALRGAVGRA"
     gene            complement(2996..>3655)
                     /locus_tag="AC16YU_02405"
     CDS             complement(2996..>3655)
                     /locus_tag="AC16YU_02405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6064100.1"
                     /translation="NAYDASAVEEALVLTEAGGGTVDLLLVGPRSAGETIRKALAMGA
                     DRATHVVVEDVGSLDSYAISGMLADELAGSGYDVIACGKQSQDTDAGLAGSMLAERLG
                     LPYASNAVALALDGDRLVVTRQGDAGQEIIALNTPCVVTCSNDMNDPRIASLKGIMAA
                     KRKEVVERDAPAVSPDRPRTVVLRHEPVPDRAAGQVFEGDPDELASTLVDRLANEARV
                     I"
BASE COUNT          599 a         1305 c         1205 g          546 t
ORIGIN      
        1 cgaaccggtg ctcgacgttg tcgccgaaga tccagagata gagcagattg ccgaacaggt
       61 gcccgaagcc gccgtgcatg aacatggccg acagtgcggt cagccagatc ggcgtgggtc
      121 cgggggcgtg cgggatgttc gcgaccgtgc cgtcgacgag gatctggtgg atgcccgtca
      181 ggtcgatccc cgtcgtgagc tcgtacggga tggcggccca gccgtatgtg aacgcattct
      241 ccgaattcgc gccctgaagc aggaagaaca ccgcggcgtt gacgagtagc agagccttcg
      301 ttacgaggga gggcctgtac agctcgcgat cgtcgtcgcc gataggaatg agcatgaggg
      361 gtttcctatc cgagattcca gttcacgccc cggcccgcct ccggatccgg aaaggaccac
      421 cggaccgctc cctggtcgca ggcgtagagg caggtgccgc actcgatgca gtcctcgaac
      481 tggaaacgga cctggccggc ctcgtccacc gtgtagcagt tcgccgggca cacgtacgta
      541 gtgcagcggt gcggacaggt cgagttgcag atcgcggtgt ccacgagaat gtgcggtcgg
      601 gcatcggtcc gaccctggtt gcggtacgcg accatgccca ggcgctcggc gatcgtggcc
      661 ttgccgctca taatgcccgc gccccccgca cgccgagctt cacgagctcc caatagctcg
      721 catgccgttt gacggccgta cggaacatgg tcgatgcacg ggccgtgggt tcgttgtcga
      781 tggtgaagaa ctggcggcca aagtcgcaca cgaccgacgg caccgacgta tgcatggccg
      841 ggtcgtgcag gagccgcaca gcatcctgga acgagcgcat gtcgcgcagc acgtagctgc
      901 cttcgagcgc ccggcggtac gaggacagca tgccggccga gaagtccccc gcctcccggg
      961 ccgagagcac gccctcagcc gcaaggatgc ccgatcgcat ggcatagtcc atgccctgga
     1021 tcgcccttcc cgcgttgagc agcaggtggg ccgcctcgcc cgcgaccagc acgccgtctc
     1081 cgaagatctc cctcggcatg ccgcgaaggt cgcccgtcga aaccacgtgg gccgagtatt
     1141 ccacgggttc cgccccgcgg atcatgtccg cgacggccgg gtgcgccttg aaggtgttca
     1201 gcacgtcgta cggcttcagg cctttctcgc gcaggtccgc catgcccagg accaggccca
     1261 gcgaaatcga ttcgaggttg gtgtacagga atcccccgcc ctcgaccccg gcggtgcacg
     1321 cgccgacgaa ttcgttcgtg aggccgctgc gtccgtgcaa ctggaaacgg tcctcgagca
     1381 cggcccggtc caggtgcaga acctccttga cgccgacggc cacgtggtcg gccggcacgt
     1441 agtcggcctc cagccccacc tggcgggtga gcaggttgtt cacgccttcg gcgagaatca
     1501 cgcagtccgc tccgaaacgc tccccgcccg cccggatgcc gaccgcccgg ccgtcctcga
     1561 gcaggatctc gtccacgagc acgttcgtag cgacgaaaga ctcgtccgcg cggccggact
     1621 ccgcgatcgc ctcctcgacc cgctccgcga gccatgcgtc gaagcgggcg cgaagcacgg
     1681 taatgccgga gtacggcgcc tcgtcgaagt gcgacgattt gaagtccacc gaaaacgccg
     1741 agccttcgtc catgaaggtc aggcgccgat gggcgacgta gcggtcccac ccggcctcct
     1801 gcccggaata cccggggacc agccgctcca ggtcgcgccc ccagagcacg cctcccgata
     1861 cgttcttcgc gccgcagaat tccccgcgct cgataaggag gaaccgcgcg ccggccctgg
     1921 ccagcgtcat cgccgcgctc aggccggcga tcccgcctcc gacaatgata cagtcgaacc
     1981 gctcgtccat caggcgcggc ccacggcccc gcgcagggcc gtgatcagcg gtggaaggat
     2041 ccggtacagg tcgccgacca gtccgtagtt cgccaccctg aagatgggcg cctcggcgtc
     2101 cttgttgatc gccacgatga cgcggctgtt tatcatgccc gccacatgct ggatcgcgcc
     2161 cgacaccccg accgcgatgt agaggtcggg cgagaccacc ttgccggtct gtccgatttg
     2221 ggcggtggcg ggaaacaggc ccgactcgac cacggccctc gacgccccga tggctccccc
     2281 gaacagtccg gcgagctcct cgacgaggcg cttgccctcc tcgtcccgca cgccgcgacc
     2341 ggccgccacg accacgcggg cctcggacag gtccaccgtc ccgcccgctc cccggacgat
     2401 ctccctgagg gtctggccga gcgatccctc gtcgaacgtg aacggcacgg ggacgacctc
     2461 ggggatcgag ggcgcctcct ccgcctcgaa cgagcccgaa cgcacggaga cgagcacggg
     2521 ctgcccctcg gcgcggaccc gtgccatctg cttcgaggcc atgaccggcc ggagggcctc
     2581 gacgccttcc gcgagcaccg tgaagtccga cacgtcgggc agggccggcg cagcgagccg
     2641 caccgagagc gctccgagca cgtccttgac ggcctcggtc gatgccatcg ccagcacgcg
     2701 ggcctccgag acccggcacc ccgcggcgag ggcctggatc atcggggcgt tcagcggaga
     2761 gcagaagacc ggatccttca ccgtgtagat gcgccgaggc ccgtaggccg ccacgccgtc
     2821 cacggccgca tccgggtcgg gaccgacgac cagcgcgtcg actgcgagac ccgcgacgga
     2881 ggcctggcga cgtgcgtgcg agaggacttc cagcgaggac cggcggatcc gcccttcgac
     2941 cgtcgaaacg aatacgagta tgctgctcat gccgatggtc ggctccggat gcttcctaga
     3001 tgacgcgggc ctcgttcgcc agccggtcca cgagggtcga ggccagctcg tccggatcgc
     3061 cctcgaaaac ctggccggcc gcgcggtcgg gaaccggctc gtgccggagg accacggtcc
     3121 gcggccggtc cggcgagacg gcaggcgcat cccgctcgac gacctccttc cgtttcgccg
     3181 ccatgatccc cttgagggac gctatgcgcg ggtcgttcat gtcgttcgag cacgtgacga
     3241 cacacggcgt gttcagagcg atgatttcct ggccggcatc tccctggcgg gtgacgacga
     3301 gccggtcccc gtccagcgcg agcgcgacgg cgttgctcgc gtacggcagt ccgagccgct
     3361 cggcgagcat cgatccggcc aggccggcgt ccgtgtcctg ggactgcttg ccgcaggcga
     3421 tgacatcgta gccgctgccg gccagctcgt cggccagcat cccggagata gcgtacgaat
     3481 ccaggctgcc cacgtcttcg accacgacgt gcgtggcgcg atcggcgccc atcgcgaggg
     3541 ccttgcggat ggtctcgccc gcggacctgg gcccgacgag caggaggtcc acggtgcccc
     3601 cgcccgcttc ggtcagaacg agggcctcct cgacggccga cgcatcgtac gcgtt
//
LOCUS       DBNHQZ010000097        12287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_704416, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000097 DBNHQZ010000000
VERSION     DBNHQZ010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_704416"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC16YU_02410"
     CDS             <1..677
                     /locus_tag="AC16YU_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6064101.1"
                     /translation="GYFLYQVAFVVGLNRTTAGSAALIMAGAPIWTALLSRLMGFEFL
                     RRLSWIGLVTSIAGTIAIVVAGSQDVELGSDVLFGNAVMLGAAFLWGSYTALNSPLLA
                     DVRPVTLTFYAVAVSLPLIVLVGLPYLLRTDWGAVTPAAWAGIAVSGALSTGLATALW
                     NTAVRTLGASHTAAYGNAVPVVALLGSALLLGEPVFPGQIVGGVLIIGGLLMLRRSRR
                     PYRPPA"
     gene            674..1468
                     /locus_tag="AC16YU_02415"
     CDS             674..1468
                     /locus_tag="AC16YU_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064102.1"
                     /translation="MNRFLLALAALLAPVLTAAGQSPYWEMAPLRFLADVDAATGHDA
                     MALSLGVDRSVPLDFFLPNVGPGSRIDAMGLGAGVRIVDGRAVFRWRVAPLELDLTRP
                     PWNGGVTLLRQDRTGLRTVEADWLAVHGGPSARVVLGTVAIEPSIVLRGGVASRRTGR
                     SFFPDLPRLADEGATGLAGSAAARILVSEPGGVSFTATASTARLWAGADPELRTLQGA
                     LRVPLNPAWSVIGRAEWHEARIVGARDRTTTFGLSIRFSPRAAGSV"
     gene            complement(1440..2030)
                     /locus_tag="AC16YU_02420"
     CDS             complement(1440..2030)
                     /locus_tag="AC16YU_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR6064103.1"
                     /translation="MHLLDVLIMVVVILGMVRGYQTGMFRQVGSIVGFLLGFFFAAGL
                     MTGAGARLTELLNVSEDIGPVLGFIAVFVVVYALTLLVVHFAEAALTQLELTTLNQVS
                     GSLFGGLFAALMASALFVLLSYVDVPAQRTRNESHLYGPTAGLAPVVWSYMSSNRDTF
                     KALTDRLDLEKLNEFVGPSHRAPKAPPPRRYQRPEG"
     gene            complement(2033..2488)
                     /locus_tag="AC16YU_02425"
     CDS             complement(2033..2488)
                     /locus_tag="AC16YU_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6064104.1"
                     /translation="MLKEQLQEDIKAAMRARDEVRLRTVRSLRAALMEREIELRTGGS
                     ATLSADQEMAVVQKQAKQRRDAAEQYRAAGRGDLVKKEEDELAIIDEYLPRQMGDEEL
                     DALVREVVKETGAESMRDMGKVMGAAMARTQGKADGKRVQAAVRTALGG"
     gene            complement(2626..3393)
                     /locus_tag="AC16YU_02430"
     CDS             complement(2626..3393)
                     /locus_tag="AC16YU_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064105.1"
                     /translation="MKTSALLVACAVLAALPVRAQEAVLRVETNDPAAHVFADSTWLG
                     TAVRGPFTLAPGLHEVRVVPSAIGTWGVPSLSFEVLAAAGDTVVVEAAFPYLYRIVSH
                     PAAEALLDAPSGVTALGGTPVVHRSGSPPEGVFRLEREGFVPALVRPGTELWNLHEVT
                     LEPVRDPDAESLGISVQEGRGPRRWIDYATVGVAVAAGVVAVHYKRKADRRYDVYRET
                     GDPTLRPAIDRYDRYSAVALGTMQVGIGVFAIRLALR"
     gene            complement(3383..4531)
                     /locus_tag="AC16YU_02435"
     CDS             complement(3383..4531)
                     /locus_tag="AC16YU_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR6064106.1"
                     /translation="MARLLLASLVALAGCQSIRLDAPVRPRSGDWTATGASTAHNTRL
                     DAAIRPPLERAWEYNAEAAFGPGGAVVVADVLLVGTLRGELHAVNLEKGKRIGVVKFK
                     EPVEGTPVLGDGRLYLATSWGDRTVHAYDLERGGKRWSVKAEPVEAGMLLVDGVLVVA
                     ATDGEVTGRDPGTGTVLWTAPSAGRPVSSTPVSTDGRAVVVADQGGLVRAIRAVDGRE
                     LWSADAGAPVYVALTAVGDCIVVPTTRGRVLGLEAGSGRTRWQYGAADSTVRFAPAAG
                     DRSRILFGATDGRVRAVDPETGELLWMAELPDGIDAQPLLAGDVVWIGTMGRELSALD
                     AGDGSVLWTTELPGRSRSSLTAHDGGLIVLSEPRTITFMKPGEAEDED"
     gene            complement(4533..7520)
                     /locus_tag="AC16YU_02440"
     CDS             complement(4533..7520)
                     /locus_tag="AC16YU_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6064107.1"
                     /translation="MAARAHTDRSRPRAGFGPAAALVLAALLAALPSAAAAQYGYHFG
                     RNKIQYDDFDWRVLETEHFDIYYYPEMKELAEQGAYFAEEGYRDLQNRFAFSLNHRVP
                     LIFYSSNLHFKQTNTTPGFIPDGVGGFFEFLKGRVVIPANGNIQRFRRVIRHELVHVF
                     TYNRLIRVMRDHRRPIDRFLPLWFTEGLAEYWSGPQDHQHEMVVRDALFSNHLVPLRN
                     IDRILGTYSMYKQGEAICRFIAEGWGEEKLLDLIDNFWKDRDFRRVVEATLRLPYDDF
                     AREWEDWMRARYYPDLEFTEMPSLIADAVSAEGFSAKPAVHRFPDGRRRVYFVGNRTG
                     YSNLYAVDVDSMYRPIGDPHAVLEGGRSDRFEAFHLFESRISISRDGHLAFVTKSGGS
                     DAIHVYDVRRERVDSTLAFEGLVAVYSPSWSPDGRSLAFTAIDDSGYSDLYVYDRDAS
                     RLERLTDDEYDDQDPAWSPDGRRIAFVSDRTDTGAEGSYNLFLYELGSGRIEHVTHGA
                     RMDFAPTWSPDGRRLAFTSAIPDSSGQYGAQDIWVADMAPEPGRFPATASLRVPEAVP
                     VRTTRTLRRITRLAGASYDPVWTQDDHLVFTSFEGLSFGIRSLARVDSLFANPVQTDE
                     VDLTRMGEPWSWPSLVEGEGYELAPYRKRFRLDLAQGVVSQSPVLGTVGGAVLAFSDM
                     MGDDYLYLTIFNTANTQREFLQSLSFQLARYQLHRRTNIGYGVYRFGGLRYDVTDPDA
                     PAGFPRFYETTYGGFGVVSYPVSQFQRIEVGTSLNWNNKEIAIRNIDRDALLLSNGVS
                     IVHDNALYWYNGPIDGWRARLDVAYTTDVLYSNVSYFSVMADVRRYWRLGRNVTFASW
                     ALARVNEGREARLWVMGGSWDLRGFRFFDVRGQKIWFTSHELRFPLLVHPTVTLPLLA
                     PFGVANLRGALFFDAAHAWNDDYDDRHPEIFAGETIGAAGFGFRLNLFGGFVLRYDLG
                     WRFRDGFKRHDKWFRQFFFGWDF"
     gene            complement(7665..9488)
                     /gene="ptsP"
                     /locus_tag="AC16YU_02445"
     CDS             complement(7665..9488)
                     /gene="ptsP"
                     /locus_tag="AC16YU_02445"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6064108.1"
                     /translation="MTARDHPGDSLSHGIGPNGEERTYMGIAVSPGIALGPAFVFERE
                     VYDVEESRIDEAEVEAEFERFQSAIARSERDLKKIVAITREKVGEDSAAIFDAQRMML
                     HDHTMHEEVVAAIRTDRVNAAWAVHRVLDRHRQKLEASDSEYFRERASDLGDVHDRII
                     RHLNRGRLLSAIDEDHVVIAENLTAADMVLFSRRGILGCAMDFGGPTSHVAIMARSLN
                     VPAVVSLHDITSSVHNGEYVILDGIRGRVIVNPTETTLARYRDRRERFGRLKEEEKEL
                     VPLAAETLDGHAVSLQANLELREELDLLPEYGAAGIGLFRTEILFLMEARLSVSEAEQ
                     FRTYRTVVERMRPGPTTFRVLDLGGDKLLPVAHREHNPFLGWRGVRVLLDRPELLMPQ
                     LRAILRASAFGPTRIMVPMVTDVTEMRAVRSMLVAAMEELRRDGVPFDEDIPLGAMIE
                     VPSAALLADRFAAECDFFSIGTNDLTQYTLAVDRGNDLVSALFRDLHPAVLRLIQSTV
                     DAARAAGIPVSVCGELANNLRAVPVLLGLGVDTLSMAPVYLPGVKRVIRAMRLDEGKE
                     LARAALDASDAEELTRRLDHWLDDHACGVSFFLEGNAANGD"
     gene            complement(9504..9773)
                     /locus_tag="AC16YU_02450"
     CDS             complement(9504..9773)
                     /locus_tag="AC16YU_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR6064109.1"
                     /translation="MLVREVTVTNRAGLHTRPASMIVRTAARFDSSFFIHKDGYEING
                     KSIIGVMTLAAEQGAVLKLVFDGDDESEAAEALAQLFEEGFGEVK"
     gene            complement(9818..10807)
                     /locus_tag="AC16YU_02455"
     CDS             complement(9818..10807)
                     /locus_tag="AC16YU_02455"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6064110.1"
                     /translation="MGAPGRPAPESPEALLRLVEEGLAQAVGSGEPAELYEAARYVLG
                     GGGKRLRPLLTLLAASHCGLPARRALGPAVAVEIFHNFTLVHDDIMDRADTRRGRPTV
                     HRRWDEPTAVLVGDLLMGIAYEMVMRGPAETRADAWAVFSPMVRRLCEGQMLDMVFAR
                     RTDVSAPEYMDMIDRKTGALLACALELGAAVAGAGEGDRAILRRVGLDLGRAFQIQDD
                     LLDLTATSASWGKPVGGDLLEGKRTWLLLAALERAEGEDRGVFERVLRGEIGEADVDG
                     VRRRMERLGVLADTRSAVIFHAEAALRGLATLEETGARGVLQDVVRGLGARTN"
     gene            complement(10811..11455)
                     /locus_tag="AC16YU_02460"
     CDS             complement(10811..11455)
                     /locus_tag="AC16YU_02460"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR6064111.1"
                     /translation="MRIALFGPPGVGKGSQASLLQERLDLRHISTGIILRRAIRSGTS
                     VGRAAEEFVTSGRLVPGHIVRALAEEAIAEAGYDDFVLDGYPRTIEQAEWLRDFLEGH
                     GSPLHGVISLRVPDQVIVDRLSKRRIDPVTGENYHLDFRPPPEDLPADRIVQREDDRP
                     DAILKRLSVYRDQTAPVEGWYRERGELREVDGVGPFEEVHTRIGAVLHALASAV"
     gene            complement(11551..12042)
                     /locus_tag="AC16YU_02465"
     CDS             complement(11551..12042)
                     /locus_tag="AC16YU_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064112.1"
                     /translation="MADRLTRSELQERIDSTEASISGRMGALQGVFRPKRLAAVQRVI
                     RLPEARIVSMVMAGLAVGWLVVGIARRFARRRRTVEHWVEDVVEEVEREKERGEDDGT
                     ALRQALRREAGAAPGVGSGSRSGLVSFVMGYAVRKGVQTLVQSLIGRWSGGRPGGNGT
                     GES"
     gene            complement(12035..>12287)
                     /locus_tag="AC16YU_02470"
     CDS             complement(12035..>12287)
                     /locus_tag="AC16YU_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064113.1"
                     /translation="LAFLALVFALVTAALGLGLWWDNMLLGFVAVSLALIVLASLVYV
                     LRPGIVRRRTGPSPEAPPAIGPAPEQPRLEAPKPEDENG"
BASE COUNT         1866 a         4391 c         4201 g         1829 t
ORIGIN      
        1 tcgggtactt cctgtaccag gtcgcgttcg tcgtcggact gaaccggacc acggccgggt
       61 cggcggccct gatcatggcg ggcgccccga tctggacggc cctcctgtcc cgcctgatgg
      121 gattcgagtt cctgcgccga ctctcctgga tcgggctcgt gacctcgatc gcgggcacga
      181 tcgcgatcgt cgtcgcgggc tcgcaggacg tggagctggg atcggacgtc ctgttcggaa
      241 acgcggtgat gctcggcgcg gccttcctct ggggctcgta cacggcactg aacagccccc
      301 ttctcgcgga cgtccggccg gtcacgctga cgttctacgc cgtggccgtc tcgctgccgc
      361 tcatcgtgct ggtcgggctt ccgtacctcc tgcgcacgga ctggggggcc gtcacgcccg
      421 cggcgtgggc gggcatcgcg gtttcgggcg cactgtcgac gggcctcgcg acggcgctct
      481 ggaacacggc cgtccggacg ctcggggcaa gccacacggc ggcctacggc aacgccgtcc
      541 cggtcgtggc gctcctgggc agcgccctgc tgctcggcga gccggtgttc ccgggccaga
      601 ttgtcggcgg agttcttatc atcgggggcc ttctcatgct ccgccgctcc cgccgaccct
      661 accggccccc ggcatgaatc gattcctcct cgcgctcgcc gcactgctcg cgcccgtact
      721 caccgcggcg ggccagagcc cgtactggga gatggccccg ctcaggttcc tcgcggacgt
      781 cgatgcggcc acgggacacg acgccatggc gctgagtctc ggggtagacc ggtcggtgcc
      841 tctcgacttc ttcctcccga acgtgggacc cggtagccgg atcgacgcga tgggcctggg
      901 cgccggcgtc cggatcgtgg acggaagggc ggtgttccgc tggcgggtcg ctccgctcga
      961 actggacctc acgaggccgc cctggaacgg gggcgtgacg ctcctccgcc aggaccggac
     1021 cggcctcagg acggtcgagg ccgactggct ggccgtgcac gggggtccgt cggcccgggt
     1081 agtgctgggg acggtggcga tcgagccgtc gatcgtactc cggggcgggg tcgcttcgag
     1141 gcgtacggga cggagcttct tcccggacct tccccggctg gcggacgagg gcgccacggg
     1201 ccttgcgggg tccgcggccg cccggatcct cgtgtcggag ccgggcggcg tctcgttcac
     1261 ggccacggcg tcgaccgcgc ggctatgggc gggcgcggac cccgagctgc gcacgctgca
     1321 gggcgcgctt cgcgtgccgc tgaacccggc gtggagcgtg atcggccgcg cggaatggca
     1381 cgaggcgcgg atcgtcgggg cgcgagaccg caccacgacg ttcgggcttt cgatccggtt
     1441 cagccctcgg gccgctggta gcgtctaggc ggcggcgcct tcggggcgcg gtggctcggt
     1501 ccgacgaact cgttcagttt ctccaggtcc agccggtccg tcagggcctt gaacgtgtcg
     1561 cggttcgacg acatgtaaga ccacaccacg ggcgcgagcc cggccgtcgg gccgtacagg
     1621 tgggattcgt tgcgcgtcct ctgcgccggc acgtccacgt acgacaggag cacgaacagg
     1681 gcgctcgcca tgagcgcggc gaagaggccg ccgaacaggg agcccgacac ctggttcagc
     1741 gtcgtcagct cgagctgcgt gagcgccgcc tcggcaaagt gcacgacgag cagggtcagg
     1801 gcgtagacga cgacgaagac ggcgatgaag cccaggaccg ggccgatgtc ctcggacacg
     1861 ttcaggagct ccgtcagccg cgcacccgct ccggtcatca gcccggccgc gaagaagaac
     1921 ccgagcagga atccgacgat cgaaccgacc tgccggaaca ttccggtctg gtagccccgg
     1981 accatcccga ggatcaccac gaccatgatc aggacgtcga ggaggtgcat cgtcagcctc
     2041 cgagcgccgt gcgcacggcg gcctggaccc gcttgccgtc ggccttgccc tgggtgcgcg
     2101 ccatcgccgc ccccatcact ttccccatgt cccgcatcga ttcggcaccg gtctccttga
     2161 ccacttctcg aaccagagca tcgagctcct cgtcgcccat ttggcgcggc aggtactcgt
     2221 cgatgatcgc gagctcgtcc tcttccttct tcacgaggtc gccccgtccg gcggcgcggt
     2281 actgctcggc cgcgtcccgg cgctgcttgg cctgcttctg gacgacggcc atttcctgat
     2341 cggcggagag cgtagctgat ccgcccgtgc ggagctcgat ctcccgctcc atcagcgctg
     2401 ccctgaggga ccgcacggtc cggagccgga cctcgtcgcg agccctcatg gcggccttga
     2461 tgtcttcctg aagttgctcc ttgagcatgg tctcgtgcct ctcgtcccgt gggcaggaat
     2521 gtccgcacgc acggccgcga tgtgtcacgc gcgcggcggg agcggacgcc gcgatcgggg
     2581 ccccggaggt gcgcgtgcgc gccgccggac tccggcctga accggtcagc ggagggcgag
     2641 gcggatggcg aacaccccga tcccgacctg catcgttccg agtgcgaccg cggagtaccg
     2701 gtcgtaacgg tcgatggccg gccgaagcgt gggatcgccc gtctccctgt acacgtcgta
     2761 gcgccgatcg gccttccgct tgtagtgcac ggcgaccacg ccggccgcga cggccacgcc
     2821 gaccgtcgcg tagtcgatcc agcggcgcgg gccgcgccct tcctggaccg agatgcccag
     2881 gctctccgca tccggatcgc gcacgggctc gagcgtgacc tcgtgcaggt tccacagctc
     2941 cgtgcccggg cgcaccagcg cgggtacgaa tccctcgcgc tccaggcgga acaccccctc
     3001 cggcgggctg cccgatcgat ggaccacggg cgtgccgccg agggccgtga cgcccgatgg
     3061 agcgtcgagg agcgcctcgg ccgcagggtg cgacacgatc cggtacaggt acgggaaggc
     3121 cgcctcgacg accaccgtgt cgccggccgc cgcaaggacc tcgaacgaga gcgacggcac
     3181 cccccatgtc ccgatcgccg agggaaccac ccggacctcg tggaggcccg gcgcgagcgt
     3241 gaacggtccc cggacagcgg tgcctagcca ggtggaatcc gcgaagacgt gggccgccgg
     3301 atcgttcgtc tcgacccgca gaacggcctc ctgggcccgg accggcaggg cggccagcac
     3361 cgcgcaggct acgaggaggg cgctagtctt catcctcggc ctcaccgggc ttcatgaagg
     3421 tgatcgtcct cggctcgctg agcacgatca ggccgccgtc gtgggcggtc agggaggaac
     3481 gcgaccggcc tggaagctcc gtggtccaga gcacggatcc gtcgccggcg tcgagcgccg
     3541 agagctcgcg gcccatggtg ccgatccaca cgacgtcccc ggccaggagc ggctgggcgt
     3601 cgatcccgtc cggcagctcg gccatccaca gcagctcgcc ggtctccggg tccactgcac
     3661 gcacccggcc gtcggtcgcg ccgaacagga tgcgcgaccg gtctcctgcc gccggagcga
     3721 agcgcaccgt cgaatccgcg gcgccgtact gccaccgcgt tcggccgctc ccggcctcca
     3781 gcccgaggac cctcccgcgg gtcgtcggca ccacgatgca gtccccgacg gcggtgaggg
     3841 ccacgtacac gggcgccccg gcatccgcgc tccagagctc gcgcccgtcg accgcccgga
     3901 tcgcccgtac caggccgccc tggtcggcga cgacgactgc ccggccgtcc gtcgacacgg
     3961 gggtcgacga gaccggacga cccgccgacg gggcggtcca caggacggta cccgtccccg
     4021 ggtcgcgccc ggtgacttcg ccgtcggtcg ccgcgacgac caggacccca tccaccagca
     4081 gcatgccggc ctccacgggc tcggccttca cgctccagcg cttgcccccc cgctcgagat
     4141 cgtaggcgtg cacggtccgg tcgccccagg acgtggcgag gtagagccgc ccgtccccga
     4201 ggacgggcgt gccttcgacg ggctccttga acttgacgac gccgatccgc ttgcccttct
     4261 ccaggttcac ggcgtgcagc tccccgcgga gcgtgccgac gagcagcacg tcggcgacga
     4321 cgaccgcacc gcccggcccg aacgccgcct cggcgttgta ctcccacgcg cgttcgagcg
     4381 gcggccggat ggcggcgtcc aggcgcgtgt tgtgggccgt cgaggcgccg gtcgcggtcc
     4441 agtcgccgct tcgtggccgc acgggggcgt cgaggcggat ggactggcag ccggccagcg
     4501 cgacgagcga tgcgagaagc agtcgggcca tatcagaagt cccagccgaa gaagaactgc
     4561 cggaaccact tgtcgtggcg cttgaagccg tcccggaacc gccagcccag gtcgtagcgc
     4621 agcacgaagc cgccgaagag gttaaggcgg aatccgaacc ccgcggctcc gatcgtctcc
     4681 ccggcaaaga tctcgggatg ccggtcgtcg tagtcgtcgt tccacgcatg cgccgcgtcg
     4741 aagaacagcg cgccgcgcag gttcgccacg ccgaacgggg ccaggagcgg caacgtcacg
     4801 gtcggatgca cgagcagcgg aaaccgcagc tcgtgcgagg tgaaccatat tttctgtccg
     4861 cgcacgtcga agaaccggaa gccccgcagg tcccagcttc cgcccatgac ccacaggcgc
     4921 gcctcgcgcc cttcgttcac gcgcgccagc gcccacgacg cgaacgtcac gttccggccc
     4981 aggcgccagt agcggcgcac gtccgccatg acgctgaagt acgagacgtt cgaatacagc
     5041 acgtccgtcg tgtaggcgac gtcgagccgg gcccgccacc cgtcgatcgg cccgttgtac
     5101 cagtagaggg cgttgtcgtg cacgatcgag acgccgttcg agaggagcag cgcgtcccgg
     5161 tcgatgttcc ggatcgcgat ctccttgttg ttccagttga gcgacgtccc gacctcgatg
     5221 cgctggaact gcgagaccgg gtagctcacg accccgaatc cgccgtacgt ggtctcgtag
     5281 aagcggggaa agccggccgg tgcgtcggga tcggtgacgt cgtagcgcag accgccgaac
     5341 cggtacaccc cgtacccgat gttcgtccga cggtgcagct ggtaccgggc cagctggaag
     5401 gagaggctct gcaggaattc ccgctgcgtg ttcgccgtgt tgaagatcgt caggtacagg
     5461 tagtcgtccc ccatcatgtc cgagaaggcg agcaccgccc cgcccaccgt cccgagcacg
     5521 gggctctggc tcaccacgcc ctgcgccagg tccagcctga accgcttccg gtacggggcg
     5581 agttcgtagc cctccccctc cacgaggctc ggccaggacc atggctcgcc catccgcgtg
     5641 aggtcgacct cgtcggtctg gacggggttc gcgaacaggg aatcgacgcg ggcgagcgag
     5701 cggatgccga aggagaggcc ctcgaagctc gtgaagacca ggtggtcgtc ttgcgtccat
     5761 acggggtcgt atgaggcgcc cgcaagccgc gtgatgcgcc tgagcgtccg cgtggtccgc
     5821 acggggacgg cctccggcac ccgcagtgaa gcggtcgccg ggaagcgtcc gggctcgggc
     5881 gccatgtcgg cgacccagat atcctgggcg ccgtattgcc cggacgagtc cgggatggcg
     5941 ctcgtgaacg cgagccgccg tccgtccggg ctccaggtcg gcgcgaagtc catgcgggcg
     6001 ccgtgcgtca cgtgctcgat gcggccggag ccgagctcgt acaggaacag gttgtacgat
     6061 ccctccgctc ccgtatcggt ccggtccgag acgaaggcga tgcgccggcc gtccgggctc
     6121 cacgccgggt cctggtcgtc atactcgtca tcggtgagcc gctccaggcg ggacgcgtcc
     6181 cggtcgtaga cgtacaggtc gctgtagccc gagtcgtcga tggccgtgaa cgcgagcgag
     6241 cgcccgtcgg gcgaccagga cggcgagtac acggccacga ggccctcgaa ggccagcgtg
     6301 ctgtccaccc gctcccggcg gacatcgtac acgtggatcg cgtccgagcc cccgctcttg
     6361 gtcacgaacg cgagatggcc gtcccgcgaa atcgagatcc gggactcgaa caggtggaac
     6421 gcctcgaagc ggtcgctccg ccctccctcg agcacggcat gtggatcccc gatcgggcgg
     6481 tacatcgagt ccacgtccac cgcgtacagg ttggagtacc ccgtgcggtt gcccacgaag
     6541 tacacgcgac ggcggccgtc gggaaagcgg tgcacggccg gcttcgcgct gaagccctcg
     6601 gccgagacgg catccgcgat cagcgacggc atctccgtga actcgaggtc cgggtagtag
     6661 cgggccctca tccagtcctc ccactcccgc gcgaagtcgt cgtagggcag acgcagcgtg
     6721 gcctccacca cccggcggaa atcccggtcc ttccagaaat tgtcgatgag gtccaggagc
     6781 ttctcctcgc cccagccctc cgcgatgaac cggcagatcg cctcgccctg cttgtacatg
     6841 ctgtacgtgc ccaggatgcg atcgatgttc ctgaggggca cgaggtgatt cgagaagagc
     6901 gcatcccgga ccaccatctc gtgctggtgg tcctgcgggc cggaccagta ctcggccagc
     6961 ccctccgtga accacagcgg caggaagcgg tcgatgggcc ggcgatgatc gcgcatgacg
     7021 cggatgagcc ggttgtacgt gaacacgtgc acgagctcgt gccggatcac cctccggaag
     7081 cgctggatgt tgccgttcgc cggaatgacc acgcggccct tcaggaactc gaagaacccg
     7141 cccacgccgt ccgggatgaa ccccggcgtc gtattcgtct gcttgaagtg gaggttggac
     7201 gagtagaaga tgagcggcac ccggtggttc agcgaaaacg cgaaccggtt ctgcaggtcc
     7261 cggtatccct cctccgcgaa gtacgccccc tgctcggcca gttccttcat ctccgggtag
     7321 tagtagatgt cgaagtgctc ggtctcgagc acgcgccagt cgaagtcgtc gtactggatc
     7381 ttgttgcggc cgaagtgata gccgtactgg gccgccgccg cggacggcag ggcggccagc
     7441 agcgcggcca gaacgagcgc agcggcgggg ccgaagccgg cccgcgggcg ggatcgatcg
     7501 gtatgggcgc gggcagccaa ggcgtcgaat ggaagggtgc cgtgcgactc gggcgatacg
     7561 gtatgatacg cgtgcggacg gggcatcgcg ggtcgcatgg aacggtcggt ccctcacgcg
     7621 cgcccggcgg gccaggaacc gccccgaacc gctttgccgg agcgtcagtc cccgttcgcg
     7681 gcgttgccct cgaggaagaa ggagacgccg caggcatggt cgtccagcca gtggtccagc
     7741 cgccgggtca gctcctcggc gtcggacgcg tcgagggcgg cgcgggcgag ctcctttccc
     7801 tcgtccagac gcatggcccg gatcacccgc ttcacgcccg gcaggtagac gggcgccatg
     7861 gacagcgtgt cgacgcccag acccagcagg accggtaccg cgcgcaggtt gttcgcgagc
     7921 tcgccgcaga cgctcacggg aatgccggct gcccgcgccg cgtccaccgt ggactggatc
     7981 aggcggagca cggccgggtg caggtcgcgg aaaagcgccg ataccaggtc gttgccccgg
     8041 tccacggcga gcgtgtactg ggtgagatcg ttcgtgccga tcgaaaagaa atcacactcg
     8101 gccgcgaacc ggtcggcgag cagggcggcc gagggtacct cgatcatcgc cccgagcgga
     8161 atgtcctcat cgaacggaac tccgtcccgg cggagttcct ccatcgccgc cacgagcatg
     8221 ctccggacgg cgcgcatttc cgtgacgtcc gtcaccatgg ggaccatgat ccgcgtggga
     8281 ccgaaggccg aagcacgaag aatcgcccga agctggggca tcaggagctc gggccggtcc
     8341 aggagcacgc gcacgccccg ccatcccagg aacgggttgt gctcccggtg ggccacgggg
     8401 agcagcttgt ccccacccag gtccaggacc cggaacgtgg tcgggccggg acgcatccgt
     8461 tcgacgacgg tgcggtaggt ccggaactgt tccgcctccg agaccgagag ccgcgcttcc
     8521 atgagaaaca ggatctccgt ccggaagagc ccgatcccgg cggcgccgta ctcgggcagg
     8581 agatcgagct cctcccggag ctccaggttc gcctgtagcg agaccgcgtg gccgtcgagg
     8641 gtttccgcgg cgaggggcac cagctccttc tcctcctcct tcaggcgccc gaagcgctcc
     8701 cggcggtcgc ggtagcgcgc gagggtggtc tcggtcgggt tcacgatcac ccgcccgcgg
     8761 atgccgtcga ggatcacgta ttccccgtta tgcaccgagg acgtgatgtc gtgcaggctc
     8821 accacggccg gcacgttcag cgaccgggcc atgatggcca cgtgcgaggt tggcccgccg
     8881 aagtccatcg cgcacccgag gatgccccgc cggctgaaga ggaccatgtc ggccgccgtc
     8941 aggttctccg cgatcacgac gtgatcctcg tcgatggccg agagcagccg gccgcggttc
     9001 aggtgacgga tgatccggtc atgcacgtcg ccaaggtcgg acgcgcgctc gcggaagtac
     9061 tcgctgtcgc tcgcctccag cttctgacgg tggcgatcca ggacgcggtg cacggcccag
     9121 gccgcgttca cgcggtcggt ccggatggcg gcgaccactt cctcgtgcat ggtgtggtcg
     9181 tgcagcatca tgcgctgggc atcgaaaatg gcggcgctgt cctctcccac cttctcgcgc
     9241 gtgatggcga cgatcttctt gaggtcccgc tccgaacgcg cgatcgccga ctggaagcgc
     9301 tcgaactcgg cctccacctc ggcctcgtcg atccggctct cctccacgtc gtacacctcc
     9361 cgctcgaaca cgaaggccgg gccgagcgcg atccccggag acacggcgat gcccatgtag
     9421 gtccgctcct ctccgttcgg cccgatgccg tgcgagaggg aatcgccggg gtggtctctg
     9481 gcagtcacgg gatctcgccg gggctatttc acttcgccga atccttcctc gaacagctgg
     9541 gccagggcct ctgccgcctc cgattcgtcg tccccgtcga agacgagctt gagcacggcg
     9601 ccctgctccg cggccagcgt catgacgccg atgatcgact ttccgttgat ctcgtatccg
     9661 tccttgtgga tgaagaagct ggaatcgaac cgggcagcgg tgcgcacgat catggacgcg
     9721 ggccgcgtat gcagtccggc ccggttcgtg acggtcactt cgcggacgag catgcgggcc
     9781 tgtacggagg gggttttcgg tggaaccggg aggagggtca attggtccgg gccccgaggc
     9841 cacggaccac gtcctgaagc accccgcgcg ccccggtttc ctcgagcgtc gcgagacccc
     9901 tcagggccgc ctccgcgtgg aatataacgg cggacctcgt gtcggccagt acgcccagcc
     9961 gttccatgcg gcgccttacg ccgtccacgt ccgcctcgcc gatctcgccc cgcaggacgc
    10021 gctcgaacac gccccggtcc tcgccctcgg cgcgctcgag ggccgcgagc aggagccagg
    10081 tcctcttgcc ctcgaggagg tcgccgccga cgggcttgcc ccacgaggcg ctcgtcgccg
    10141 tgaggtccag caggtcgtcc tggatctgga aggcgcggcc caggtcgagc ccgacgcgac
    10201 gcaggatcgc ccggtcgccc tcgccggccc cggccacggc cgcgccgagc tccagggcgc
    10261 acgcgagcag cgcgccggtc ttccggtcga tcatgtccat gtactccggg gccgagacgt
    10321 ccgtccgccg ggcgaagacc atgtccagca tctgcccctc gcacaggcgg cgaaccatgg
    10381 gcgagaacac ggcccaggcg tcggcgcgag tctccgcggg cccccgcatg accatttcgt
    10441 aggcgattcc catgagcagg tcgcccacga ggaccgccgt cggctcgtcc cagcgcctgt
    10501 gcacagtggg gcggccgcgc cgcgtgtccg cccggtccat gatgtcgtcg tgaaccaggg
    10561 tgaaattgtg gaagatctcc accgccacgg cagggccgag cgcgcgccgt gccgggagcc
    10621 cgcaatgcga agcggcgagc agcgtgagca gcgggcgcag gcgctttccg ccgcccccga
    10681 gtacgtaccg ggcggcctcg tagagctcgg cgggttctcc cgatcccacg gcctgcgcga
    10741 ggccctcctc gaccaggcgg agaagcgctt cgggggattc gggggccgga cggcccgggg
    10801 cgcccacggc tcagaccgcg ctcgcgagcg cgtggagcac ggcaccgatg cgcgtgtgca
    10861 cctcctcgaa cggccccact ccgtccacct cccggagttc gccccgctcg cggtaccagc
    10921 cctccaccgg agcggtctgg tcccggtaca cggagaggcg cttcaggatc gcatccggac
    10981 ggtcgtcctc gcgctgcacg atccggtccg ccggcaggtc ctcgggcggg ggccggaagt
    11041 ccaggtggta gttctcgccc gtgaccggat cgatccggcg tttcgacaac cggtccacga
    11101 tgacctggtc cggcacccgc aggctgatca cgccgtggag cggggagccg tgcccctcca
    11161 ggaaatcgcg cagccactcg gcctgctcga tggtgcgcgg atagccatcc agcacgaaat
    11221 cgtcgtaccc cgcctcggcg atggcctcct cggccagcgc gcgaacgatg tggcccggca
    11281 cgagccgccc ggatgtcacg aattcctccg cggcacggcc gacggacgtg cccgagcgga
    11341 tggcccgacg aaggatgatc cccgtcgaga tatggcgaag atccaggcgc tcctgcagga
    11401 ggctggcctg cgagcccttg cccacgccgg gcggtccgaa cagtgcgatg cgcatcgacc
    11461 tcacgccgcc ggagcgaatc cggcggccgg taccctctgg aaaaaaaaga gcgtcgaaga
    11521 tacggccgcg gccatcgggc cgccaccgga tcaggattct ccggtgccgt tgccgcccgg
    11581 tcgcccgccc gaccagcggc cgatcaggct ttgcacgagt gtctgcacgc ctttccgaac
    11641 ggcgtatccc atgacgaagg acaccaggcc cgaacggctt ccgctcccga cccccggggc
    11701 agcccctgct tccctccgca gggcctgccg gagcgccgtc ccgtcatcct ccccccgctc
    11761 cttctcgcgc tcgacctcct ccaccacgtc ttccacccag tgttcgaccg tgcggcggcg
    11821 ccgagcgaac cgcctcgcga ttcccacgac caaccagccc acggcgagcc cggccatgac
    11881 catcgacacg atccgcgcct ccggaagccg gatcacgcgc tggacggcgg caagccgctt
    11941 cgggcggaac acgccctgga gagcgcccat ccgcccggag atcgaggcct ccgtgctatc
    12001 gatacgctcc tggagctcgc tgcgggtaag ccgatcagcc attttcgtcc tccggtttcg
    12061 gcgcctcgag gcgcggctgc tccggcgccg gaccgatcgc gggcggcgcc tcgggcgaag
    12121 ggcccgtccg gcgtcggacg atgcccggcc tgagcacgta tacgagagac gcgaggacga
    12181 tgagcgcaag cgacacggcc acgaagccga gcagcatgtt gtcccaccac aggccgaggc
    12241 cgagcgcagc cgtgacgagc gcaaaaacca gggccaggaa ggccagg
//
LOCUS       DBNHQZ010000098         5699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1110576, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000098 DBNHQZ010000000
VERSION     DBNHQZ010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1110576"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC16YU_02475"
     CDS             complement(<1..447)
                     /locus_tag="AC16YU_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6064114.1"
                     /translation="MQTLVHSAGFAFMAFLILAAGCGQGSREAGPAAYDPASDSLRFA
                     GETHLRNIRQLTFGGNNAEAYWSPDGTQLTFQSDFSGLTGQGCDQIFVMNADGSSRPS
                     GRRYDRVSTDTGRTTCSYFMPGGRVLFASTHAAAPECPAEVMFEDGR"
     gene            536..1351
                     /gene="trxA"
                     /locus_tag="AC16YU_02480"
     CDS             536..1351
                     /gene="trxA"
                     /locus_tag="AC16YU_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6064115.1"
                     /translation="MAFEVEQFEQDVIEASRKQPVLVDFWAPWCGPCRVLGPVLEKLA
                     GEADGAWTLAKVNSDEHPELSARYGIRGIPAVKLFADGQVVDEFTGALPEYAVRQWLE
                     KALPSETRSWLARAESALEEGEADEARALLEAILRAEPTHPAASALMARLIVFDDPDR
                     ALSLAETAMAAEARYVQMAESVRNLAAVLAAAHEPEKLPDEPGRDAYRTAMDALRAGD
                     YEGAIGSLIQVLQQNRYYADDGARKAGVAIFTLLGDRHPVTRAHRRTFDMWLY"
     gene            complement(1355..3160)
                     /locus_tag="AC16YU_02485"
     CDS             complement(1355..3160)
                     /locus_tag="AC16YU_02485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064116.1"
                     /translation="MSDTDKEKDGPGGRGFDRRLLRRIVTYLVPYKGWVALAFVTVMT
                     EAFLGPLVPKLVQLAIDRHIVVGDLPGLRRMILLLVIVLVAEGVLSFVNQYLTQWIGQ
                     QAIYDLRTRVHRHILRQPVAFFNRTPIGRLLTRVTSDVESLSQVLSAGVVTILGDLSR
                     LVFIAWFMFSLNWVLALVTLAVMPVMLWATFWFTRKVRDAYRDTRKQAARLNSFLQEH
                     VTGMRVVQMFNREKEEYRRFEGINDDHRQAYLKTIFYFALFWPVVDIIGSSALGLVIW
                     VGGVRAMEGALTLGVLIAFIQYARQFFEPIRNLSDQFNTLQSAMAGAERIFGLLDQDD
                     ALPEREDPVPLPTVRGEIEFRNVWFAYVDGGEGEPEWVLRDVSFVLRPGQTAAIVGAT
                     GAGKSTIINLLLRFHDVQKGQILVDGEDIRNYRLRDLRGHVGLVLQDVFLFSGSIERN
                     ISLDNPDIPREAVVEATRQIGADEFIGRLKNGYAQDVRERGMTLSHGQRQLLSFARAL
                     AYDPEILVLDEATSSVDTETEHVVQEALHRLMKGRTSLVIAHRLSTIQHADQILVMHR
                     GEIRERGSHQELLRADGLYRRLYELQYKEQEASAA"
     gene            complement(3173..4975)
                     /locus_tag="AC16YU_02490"
     CDS             complement(3173..4975)
                     /locus_tag="AC16YU_02490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064117.1"
                     /translation="MGPLAPVNRYYWTYRRLFIPGLLCAVAASGFAIVVPIVVRYSVD
                     GIPRMVASYRAFEGSPVGGWLHGYFFLALVVTAVIILLLSILSGTFSFLMRQTVVVAS
                     RHIEFDLRNRLFDHLQQLSRGFYVRHSTGDLITRATSDIEQVRRYVGPALMYTTRAVV
                     TIVTAMTVMFVISPRLTLFALIPMPFLALSVFFVARLVHQRSDALQQQYSSLTSRVQE
                     ALSGIRVIKAYSREEAEQEGFDAESSGYRKRSLDLALVEAAWRPVFVLLVGLATIIVV
                     WEGGRLVVVGAITIGNIAEYIIYVAMMTWPVAAFGFVISMVQRASASMVRLQAIFDAE
                     PDIRDGERTDAGVREVEGAIEFDGVGYTFEGDEEPALEGVSFSVPAGTTLGIVGRTGS
                     GKTTLVEMIPRLLQPTVGRVLVDGRDAREIPLHALRSSIGYVAQDVFLFSDTIAENIA
                     FGRMEAPIEAIERAAAEADLLENVKDFAHGFETLVGERGITLSGGQKQRSAIARALVC
                     EPRILILDDALSAVDVQTEQTILANLRRQFGRRTIVIVSHRISAVQEADQIVVLDAGR
                     VVEQGAHDELLAREGLYASLYRKQLLEQELEAMA"
     gene            5142..>5699
                     /locus_tag="AC16YU_02495"
     CDS             5142..>5699
                     /locus_tag="AC16YU_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6064118.1"
                     /translation="MLEQPARNLDLLERENMQLRRAVDELSILNELSAAIGASRDVQE
                     VIQTIIRRSLKAVRAEQGVITLVGEEATDPTRTLVRTMASSGETEAYSPDQNLLGWMH
                     LHKRPLVINDPRSDDRFRGVRWPESVRSIVSVPMIAQSRLIGILTLYNKKRDDAGFSG
                     EDQRLLAILAAQSAQIVENARLYQEE"
BASE COUNT          957 a         1968 c         1920 g          854 t
ORIGIN      
        1 gcggccgtcc tcgaacatga cttcggccgg acactcgggc gccgccgcgt gcgtggacgc
       61 gaacagcacc cggccgcccg gcatgaagta gctgcaggtc gtccgcccgg tgtcggtcga
      121 cacccggtcg taacgccggc cgctcggccg cgacgagccg tccgcgttca tgacgaagat
      181 ctggtcgcag ccctgtcccg tcaggccgct gaagtccgac tggaacgtga gctgcgtgcc
      241 gtcgggactc cagtaggcct cggcgttgtt tccgccgaag gtcagctgcc ggatgttccg
      301 caggtgcgtc tcgcccgcga accgcagcga gtccgaggcg ggatcgtagg ccgcggggcc
      361 cgcttcccgg ctgccctggc cacagcctgc ggccagaatc aaaaacgcca tgaacgcgaa
      421 tcctgcgctg tgaacgaggg tttgcatgat cggtcgggat agtgtcgggt gtcggttgcg
      481 gcgccgcgaa actacgatcc cggccccgca gccaccatga aaaagcgcga gagccatggc
      541 gttcgaggtt gagcagttcg agcaggacgt gatcgaggcc agcaggaagc agccggtgct
      601 ggtggacttc tgggccccgt ggtgcgggcc gtgccgggta ctggggcccg tcctggagaa
      661 gctcgccggc gaggccgacg gcgcctggac cctcgcgaaa gtgaactcgg acgagcatcc
      721 ggagctctcg gcccgctacg gcatccgcgg cattcccgcc gtgaagctgt tcgccgacgg
      781 gcaggtggtc gacgagttca cgggcgccct cccggagtac gccgtccgcc agtggctcga
      841 gaaggcgctc cccagcgaga cgcggtcgtg gctcgcccgg gcggaatcgg ccctcgagga
      901 gggcgaggcc gacgaggcga gggccctgct cgaggccatc ctccgcgccg agccgaccca
      961 ccccgccgcg agcgcactca tggcccgcct cattgtgttc gacgacccgg accgggcgct
     1021 ctccctggcc gagaccgcca tggccgcgga ggcgcgttac gtgcagatgg ccgagtccgt
     1081 ccgcaacctg gccgccgtcc tcgcggcggc gcacgagccg gaaaagctcc cggacgagcc
     1141 gggacgggat gcgtaccgga cggccatgga cgccctgcgc gccggggatt acgagggcgc
     1201 gatcggcagt ctcatccagg tgcttcagca gaaccgctac tacgcggacg acggcgcccg
     1261 caaggcgggt gtcgcgatct tcacgctcct cggcgacagg cacccggtga cccgcgccca
     1321 ccggaggacc ttcgacatgt ggctgtactg accgtcacgc cgcgctcgct tcctgctcct
     1381 tgtactggag ctcgtacagg cgccggtaca ggccgtcggc gcgcagcagc tcctggtgcg
     1441 agccgcgctc gcggatctcg ccacggtgca tcaccaggat ctggtcggcg tgctggatgg
     1501 tggacagccg gtgcgcgatg acgagcgagg tccgcccctt catcagccgg tggagcgcct
     1561 cctgcaccac gtgctcggtc tcggtgtcca cgctcgacgt ggcctcgtcc aggaccagga
     1621 tctccgggtc gtaggccagc gcgcgcgcga acgagaggag ctgccgctgc ccgtgcgaca
     1681 gcgtcatgcc gcgctcgcgc acgtcctgtg cgtagccgtt cttcaggcgc ccgatgaact
     1741 cgtccgcgcc gatctgccgc gtcgcctcga cgaccgcctc gcgcgggatg tccgggttgt
     1801 ccaggctgat gttgcgttcg atcgatccgg agaacaggaa gacgtcctgc agcacgagac
     1861 ccacgtggcc gcggaggtcg cggagccggt agttccggat gtcctcgccg tcgaccagga
     1921 tctgtccctt ctgcacgtca tggaagcgca gcagcaggtt gatgatggtc gactttccgg
     1981 ccccggtcgc gcctacgatg gccgccgtct ggccgggccg caggacgaac gacacgtccc
     2041 ggagcaccca ctcgggctcc ccttccccgc cgtccacgta cgcgaaccac acgttgcgga
     2101 actcgatctc gccgcgcacg gtcggcagcg ggaccggatc ctcgcgctcg ggcagggcgt
     2161 cgtcctggtc cagcagcccg aagatgcgct cggcgccggc catcgccgac tggagcgtgt
     2221 tgaactggtc cgacaggttt cggatgggtt cgaagaactg ccgggcatac tggatgaagg
     2281 cgatcagcac gccgagcgtg agcgcgccct ccatcgcccg cacgccgccc acccagatca
     2341 cgagccccag cgcgctggag ccgatgatgt ccacgaccgg ccagaagagc gcgaagtaga
     2401 agatcgtctt caggtacgcc tgccggtgat cgtcgttgat cccttcgaag cgccggtact
     2461 cctccttctc ccggttgaac atctggacca cccgcatgcc cgtcacgtgc tcctgcagga
     2521 acgagttcag gcgtgcggcc tgcttgcgcg tgtcgcggta ggcgtcgcgc accttgcgcg
     2581 tgaaccagaa ggtggcccac agcatgacgg gcatgacagc cagcgtcacg agggcgagca
     2641 cccagttcag cgagaacatg aaccaggcga tgaagaccag gcgagagagg tcgcccagga
     2701 tcgtgaccac gccggccgag agcacctggc tcagggattc gacgtccgag gtcacgcgcg
     2761 tgaggaggcg gccgatcggg gtccggttga aaaacgcgac cggctgacgg aggatgtgcc
     2821 ggtgcacgcg ggtgcgtagg tcgtagatgg cctgctgccc gatccactgg gtcaggtact
     2881 ggttgacgaa cgacagcacg ccctccgcga ccagcacgat gacgagcagc aggatcatcc
     2941 gccgcaggcc gggcaggtct ccgaccacga tgtggcggtc gatggccagc tggaccagct
     3001 tcggaacgag cggccccagg aacgcctccg tcatcaccgt gacgaacgcc agcgccaccc
     3061 agcccttgta cgggaccagg taggtgacga tgcgccggag caggcggcgg tcgaaaccgc
     3121 gcccgcccgg gccgtccttc tctttgtcgg tgtctgacac gggcgtccgg tctcaggcca
     3181 tggcctcgag ttcctgttcc aggagctgct tccggtagag cgacgcgtac agtccctcgc
     3241 gggcgagcag ctcgtcgtgg gcgccctgct ccacgacgcg gccggcgtcg aggacgacga
     3301 tctggtcggc ctcctgtacg gccgagatgc ggtggctcac gatcacgatc gtgcgccggc
     3361 cgaactggcg gcgcaggttg gcgagaatcg tctgctccgt ctgcacgtcc accgcggaca
     3421 gggcatcgtc caggatcagg atcctgggct cgcatacgag cgcccgggcg atggcggacc
     3481 gctgcttctg cccgcccgac agcgtgatgc cgcgctcgcc gacgagcgtc tcgaagccgt
     3541 gggcgaagtc cttcacgttt tcgagcaggt cggcctcggc agcggcccgc tcgatggcct
     3601 cgatcggcgc ctccatgcgc ccgaacgcga tgttctcggc gatcgtgtcc gagaagagga
     3661 agacatcctg cgccacgtaa ccgatcgacg acctgagcgc atgcagcggg atctcgcggg
     3721 cgtcccgtcc gtcgacgagc acgcgtccga cggtcggctg gagcaggcgc ggaatcatct
     3781 cgacgagggt ggtcttgccg gagcccgtgc gtcccacgat gccgagcgtc gtgccggccg
     3841 gaacggaaaa gctcacaccc tcgagtgccg gctcctcgtc cccctcgaac gtgtatccca
     3901 caccgtcgaa ctcgatcgcg ccttccacct cgcgcactcc cgcatccgtt cgttcgccgt
     3961 cccggatgtc cggctccgcg tcgaagatcg cctggagccg caccatggac gccgatgcgc
     4021 gctgcaccat cgagatcacg aagccgaacg ccgccacggg ccaggtcatc atcgccacgt
     4081 agatgatgta ctcggcgatg ttgccgatcg tgatggcccc gaccacgacg agccggccgc
     4141 cctcccagac cacgatgatg gtggcgagcc ccaccaggag cacgaacacc ggacgccatg
     4201 cggcctcgac cagggcgagg tccaggctgc gcttccggta cccggaggac tccgcgtcga
     4261 agccctcctg ctcggcttcc tcccgggaat aggccttgat cacgcggatc cccgagagcg
     4321 cctcctgcac gcggctcgtg agcgaggagt actgctgctg cagcgcgtcg gagcgctggt
     4381 ggaccaggcg cgccacgaag aacacagaca gcgcgaggaa gggcatcggg atgagcgcga
     4441 aaagcgtgag ccgcggcgag atcacgaaca tcaccgtcat cgcggtcacg atggtcacca
     4501 cggcgcgggt cgtgtacatc agggccggac ccacgtagcg ccggacctgc tcgatgtcgc
     4561 tcgtggcgcg cgtgatcagg tcgcccgtcg agtgccggac gtagaagccg cgagagagct
     4621 gctgcaggtg gtcgaagagc cggttgcgca ggtcgaactc gatgtggcgg gaggcgacga
     4681 ccaccgtctg tcgcatcagg aaggagaacg tgccgctgag gatgctcagg agcaggatga
     4741 tcacggccgt cacgacgagg gccaggaaga agtagccgtg gagccagccg ccgaccggcg
     4801 acccctcgaa ggccctgtag ctcgccacca tgcgcggtat gccgtccacc gagtagcgta
     4861 ccacgatcgg caccacgatc gcgaagcccg acgcggcgac ggcacagagc aggcccggaa
     4921 tgaagagccg tcggtacgtc cagtagtacc ggttgaccgg ggcgagagga cccatggaat
     4981 ccgtggggga gcgaatttct gcaaacgccg gccaccgacc ggtgatccgc gcctccgcgt
     5041 cgagtccgcc gcccggtgcg cccaaacctt atttgaccgc gctcgcacgt ttacgctacc
     5101 ttcacacgct tactccttcc cccgccaagc caccgccctc cgtgctcgaa cagcctgcca
     5161 ggaacctgga tctcctcgaa cgcgagaaca tgcagctgcg ccgcgcggtc gacgagctct
     5221 cgatcctcaa cgagctctcg gccgccatcg gcgcgtcccg cgacgtccag gaggtcatcc
     5281 agacgatcat ccggaggtcc ctgaaggccg tgcgcgccga gcagggggtg atcacgctcg
     5341 tcggcgagga ggcgaccgat ccgacccgca ccctggtccg cacgatggcc agttcggggg
     5401 aaaccgaggc gtacagcccc gaccagaacc tgctggggtg gatgcacctt cacaagcgtc
     5461 cgctcgtcat caacgatccc cggagcgacg accggtttcg gggcgtccgc tggcccgagt
     5521 ccgtgcgctc gatcgtgagc gttccgatga tcgcccagtc ccgcctgatc ggcattctca
     5581 cgctctacaa caagaagcgg gacgacgcgg ggttctcggg cgaggaccag cggctgcttg
     5641 ccatcctcgc ggcccagtcg gcccagatcg tcgagaacgc ccgcctgtac caggaggag
//
LOCUS       DBNHQZ010000099         5909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1111107, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000099 DBNHQZ010000000
VERSION     DBNHQZ010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1111107"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="AC16YU_02500"
     CDS             <1..2284
                     /locus_tag="AC16YU_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064119.1"
                     /translation="AQPVPAPAPRSAESASTGRIIGVFGVMATLAIAVVYAAMHYFGL
                     PDWVLAVGVLLMAIGLPILILAGAMERRRATLDSGERSALSGVPGWLSVRKAAIGGVG
                     AMLLLAVASGGWMGMRAMGIGPAGTLVSKGALDARDFVVLADFENRTSDPNLAVSIKE
                     AFRIDLSQSSIVNLVDAATVRGALERMQRDPDSPLGAAVAREVAERTGAKAVILGDIS
                     SVGRSFVVVARLVSASDGSELVALRENAADDGALLPAIDRLSGRLRERVGESLVTIRG
                     GEPLEDVSTASLPALRAYSEAEAAASGGGDYGRAEELLLEALDLDSTFAMAWRKLAVV
                     RANAGASLADRNAAAERAYALRDRLPERERLLADAAYFNYVVPDDDRVMAAYEAVLEK
                     WPDDLTALNNLSIEYGDRNRFEDAERLLRRAVEIGESITFHQNLIGALANQRKWDEVD
                     ERITEFIGLYPENALPWTWQARLAARRSEFERAEALLDSASARVRDPADGDFLMRTRG
                     TIAQLVGRYQEAQRHFDELSAGLADRGFEGAQIGTRLWPALLRADVSGDMEWLRNYLD
                     GLLERYPLAGMNVRTRPDPFVAGMYAWTGDPDRAEAILAQWRSEVPAQLRPDEPYVSM
                     ALIALARGDRAGALELVEKEREASDCTECVWDAQARMYERMDSTEAAVRAYERFLASD
                     RGFNLLEEEVPVRLRLGDLYQDLGDLDRAIENLGRVVTLWAGADPDLQPSVRQAQADL
                     DRLLDLKAREPATVPATSRP"
     gene            2316..3074
                     /locus_tag="AC16YU_02505"
     CDS             2316..3074
                     /locus_tag="AC16YU_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR6064120.1"
                     /translation="MYVRFVKLRVKPGMEAAYDRFYRAEVAPAVLENEGCLFAHLIQD
                     EETPTAFVSLTFWRSPEDAAGYEQSGRFAALVERNRPFLDDSSEWRMVLKEDLTLSYV
                     PVMIEPVVEAFDVTAASTDQAPDERGLAHMYFRIVAGHADPDRIEELRQVFRDEVIDA
                     LRDVDGCRYAYLAAGRAENEVISVTLWDSRAQALAYESSRLYLDIMAKLRPMMSSLTQ
                     WKATLDPERQSNVTTSDDLEVRGFRVPGADTGEA"
     gene            complement(3081..3737)
                     /locus_tag="AC16YU_02510"
     CDS             complement(3081..3737)
                     /locus_tag="AC16YU_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6064121.1"
                     /translation="MTRPHAIIVGAGPGMGLALARRFGAGGFRISLVARRQETLNRFV
                     ASLRTRRNAAAGYVADAGDWHGLADTIAAAEKEHGPCEVLCYNPAGRIVSPLADVEPE
                     VLVDTLRVNVVGALAAVRAVLPGMRERGRGSILFTGGGLAVRPLPKFGALAIGKAAQR
                     SMVQTLSAELQGSGIYVGTVLIAGYVQGGTDFDPDRIAEEFWRLHTEHPSESEVLFRG
                     "
     gene            complement(3914..4552)
                     /locus_tag="AC16YU_02515"
     CDS             complement(3914..4552)
                     /locus_tag="AC16YU_02515"
                     /inference="COORDINATES: protein motif:HMM:NF042765.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HIR6064122.1"
                     /translation="MSIRSVAWLFAVGLGAATAQPPAVAAPDPRAGVTSEPAAAAAPD
                     PPAAAVPDSEAASAHEFHVTYGRMGVEGAIIQVRLRFFQDDLALALRAFAGDPDVLLA
                     VDPGSDSLVTAYLADRFPVRAGAGDDVSGTDPLAGSIVASGEEVDGAERIWWYVLQYE
                     AAAPIGRVRVDAKVLLELFDDQRNILRIQAFPSGRERTYYLTHGSSVADFVV"
     gene            complement(4552..>5909)
                     /locus_tag="AC16YU_02520"
     CDS             complement(4552..>5909)
                     /locus_tag="AC16YU_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR6064123.1"
                     /translation="TVPWNHILDATGTLQNPEEVLTATQRERLERAYTSEEPVFIVPA
                     DEVGDPVARPVTSGTVTWHFKANNVRDFAFASSRAFVWDAAGFRYRASDDPILIHSLY
                     PKEAMPLWDKVSTRATIQTMKTYGRMAFEYPYEKAINVNGPVGGMEYPMVAFCGARPA
                     ADGTYSDQVERALIGVTIHEVGHNWFPMIVASDERKWTWMDEGLNTFLQYYGEIDYAR
                     TYCGTYFTQTDDCTYPSSRGPAENIVDYMRDPDQVPIMTESDLIHKDFGNNGYAKPAT
                     GLVMLREHILGPEAFDQAFSGYSQGWAFRHPMPADFFRSMDEGAGENLAWFWRGWFYT
                     THANDQAIESVSSQPADSLLGSSERGAYYYRVKVSNEGGLLMPLELEVTYDDGSTERM
                     EMPADVWRNNELEYTKGFFSDRKVNRVVLDPDAAYADINPDNNTWIAPELRETGEQGN
                     G"
BASE COUNT          897 a         2036 c         2071 g          905 t
ORIGIN      
        1 cgcgcagccc gtccccgcgc cggccccgcg ctcggccgag tcggcctcga cgggtcgcat
       61 catcggagtt ttcggggtca tggccacgct cgccatcgcc gtggtgtacg cggcaatgca
      121 ctacttcggc cttcccgact gggtgctggc ggtcggcgtg ctgctgatgg cgatcggcct
      181 ccccatcctg atcctcgccg gcgccatgga gcgtcgtcgc gccacgctcg attcgggcga
      241 gcggagcgcc ctctccggcg ttccgggctg gctctcggtc cgcaaggccg cgatcggagg
      301 ggtcggcgcc atgctgctcc tggccgtcgc gtccggcgga tggatgggca tgcgcgcgat
      361 ggggatcggg ccggcgggca cgctcgtctc gaagggtgcg ctcgacgcgc gggacttcgt
      421 ggtcctggcg gacttcgaga accggacgtc cgatccgaat ctcgccgtat cgatcaagga
      481 ggccttccgc atcgaccttt cccagtcgtc gatcgtgaac ctggtggacg cggcgaccgt
      541 gcgcggcgcc ctggagcgga tgcagcgcga tccggactcg ccgctcgggg cggccgtcgc
      601 gcgcgaggtc gcggagcgga cgggggccaa ggccgtcatc ctcggcgaca tctcgtccgt
      661 cgggcgcagc ttcgtcgtgg tggcccggct ggtgagcgcc tccgacgggt cggagctcgt
      721 ggcgctccgc gagaacgcgg cggacgacgg cgccctgctc ccggccatcg accggctctc
      781 cgggcgcctg cgggagcggg tcggcgagtc cctcgtgacc atccgcggcg gcgaaccgct
      841 cgaggacgtg tcgacggcct cgctgcccgc actccgcgcc tattccgagg ccgaggccgc
      901 cgcgagcggc ggcggtgact acgggcgtgc agaggagctg ctgctcgagg ccctcgacct
      961 cgattccacg ttcgccatgg cgtggcgcaa gctggccgtg gtgcgggcaa acgccggcgc
     1021 ttcgctcgcc gaccggaacg cggcggccga aagggcctac gcgctccggg accgtctccc
     1081 cgagcgggag cggctgctcg cggacgccgc ctacttcaac tacgtggtcc cggacgacga
     1141 ccgggtgatg gccgcctacg aggccgtgct cgagaagtgg ccggacgacc tcacggcgct
     1201 caacaacctc tccatcgagt acggcgaccg gaaccgcttc gaggacgcgg agagactgct
     1261 ccggcgcgcc gtcgagatcg gcgagagcat cacgttccac cagaacctga tcggcgcgct
     1321 cgccaaccag cgcaagtggg acgaggtcga tgagcggatc accgagttca ttgggctcta
     1381 tccggagaat gccctgccgt ggacctggca ggcccggctg gccgccaggc ggtcggagtt
     1441 cgagcgtgcc gaggccctgc tcgactccgc ctccgcgcgg gtccgggacc cggcggacgg
     1501 ggacttcctc atgagaacgc gggggaccat cgcacagctc gtcgggcggt accaggaggc
     1561 gcagcggcac ttcgacgagc tgtcggcggg cctggccgac cggggcttcg agggagccca
     1621 gatcggaacc cggctctggc ccgccctgct tcgggcggac gtcagcggcg acatggaatg
     1681 gctccggaac tacctggatg gtctcctcga gcggtatccc ctggccggga tgaacgtgcg
     1741 gacccggccg gatccgttcg tcgcaggcat gtacgcgtgg accggcgacc cggaccgggc
     1801 ggaggcgatc ctcgcacagt ggcggtccga ggtcccggca cagcttcgac cggacgagcc
     1861 gtacgtgagc atggccctga tcgcgctggc ccggggcgac cgcgcgggag cgctcgaact
     1921 cgtggagaag gagcgggagg caagcgactg caccgaatgc gtctgggatg cccaggcccg
     1981 catgtacgag cgcatggatt cgaccgaggc ggccgtgcgc gcctacgaac gcttcctcgc
     2041 cagcgatcgg ggcttcaacc tcctcgagga ggaggtccct gtccggctcc ggctcggcga
     2101 tctctaccag gacctcgggg acctggaccg ggccatcgag aatctcggac gggtcgtcac
     2161 gctgtgggcc ggcgccgacc cggacctcca gccctccgtg aggcaggccc aggccgacct
     2221 ggatcgcctc ctcgacctga aggcccgcga gcccgcgacc gtgcctgcga cctcgcgacc
     2281 ctgatccgtc cccgttgcac ctaccccctc ctgccatgta cgtccggttc gtcaagctgc
     2341 gcgtgaaacc cggcatggag gccgcctacg accgcttcta ccgggccgag gtcgccccgg
     2401 ccgtgctgga gaacgagggc tgcctgttcg cccacctgat ccaggacgag gagacgccga
     2461 cggccttcgt ctcgctgacc ttctggaggt cgccggagga cgccgccgga tacgagcaga
     2521 gcgggcgctt cgccgcgctg gtcgagcgca accggccctt cctggacgat tcgtcggagt
     2581 ggcggatggt cctcaaggag gatctcacgc tctcgtacgt gccggtcatg atcgagccgg
     2641 tggtcgaagc cttcgacgtg acggccgcga gcacggacca ggctcccgac gagcgcggcc
     2701 ttgcccacat gtatttccgg atcgtggccg ggcacgccga tcccgaccgg atcgaagaac
     2761 tccgacaggt tttccgagac gaggtgatcg acgcgctccg ggacgtggac ggctgccgct
     2821 atgcgtacct tgccgcgggc cgggcggaga acgaagtgat atccgtgacc ctgtgggaca
     2881 gccgggcgca agcgctggcg tacgagtcca gccggcttta cctggacatc atggcgaagc
     2941 tgcggccgat gatgtcgtcg ctcacgcagt ggaaggcgac gctcgatccg gaacggcagt
     3001 ccaacgtcac gacgtcggat gacctggagg tccgtggctt ccgcgtgccg ggcgcggaca
     3061 ccggggaagc ctgagagcga tcagccgcgg aagaggacct cggattcgga agggtgctcg
     3121 gtgtgcagcc gccagaactc ctccgcgatc cggtccgggt cgaagtccgt gcctccctgg
     3181 acgtatcccg cgatcaggac cgtgcccacg tagatgccgg aaccctggag ttcggccgag
     3241 agcgtctgga ccatgctccg ctgggccgcc ttgccgatcg cgagggcgcc gaacttcggc
     3301 agtggtcgca cggccagccc cccgccggtg aacaggatcg agccccggcc ccgctcccgc
     3361 atgccgggca ggaccgcacg caccgccgcg agtgcaccga ctacgttcac acgcagggtg
     3421 tcgacgagca cctcgggctc gacgtccgcg agcggcgaca cgatgcggcc cgcggggttg
     3481 tagcagagca cctcgcaggg cccgtgctcc ttctcggcgg ccgcaatggt gtccgcgagg
     3541 ccgtgccagt cgccggcgtc cgccacgtag cccgccgcgg cattccgacg cgtgcgaagg
     3601 gacgccacga agcggttcag agtctcctgg cggcgggcga ccaggctgat gcgaaagccg
     3661 ccggctccga accggcgggc gagggcgagc cccatgccgg ggccggctcc gacgatgatg
     3721 gcgtgcggac gcgtcatgga cgtcgattcg atggcgctta cgattcgagg gcggggcgtt
     3781 tacccgactt ccggccggga gttcgcccgc cgcccaacga taaggccccc ggcgccgcga
     3841 tcggcccttg taccgaaatt cacgaggcgc gcggacccgg gagaaggtgc cggaacgcgc
     3901 ggcgaggtgc agctcagacg acgaaatcgg cgacggacga accgtgcgtc aggtagtacg
     3961 tccgttccct tccggatggg aaagcctgga tccggaggat gttgcgctga tcatcgaaga
     4021 gctcgagaag caccttcgca tcgacccgga cgcgcccgat cggggccgcc gcctcgtact
     4081 gcagcacgta ccaccagatg cgttcggccc cgtcgacctc ctccccgctc gcgacgatcg
     4141 agcccgcgag cggatccgtt ccggaaacgt cgtctcccgc cccggcacgg accgggaagc
     4201 ggtccgccag gtacgccgtg acgagcgaat ccgaacccgg gtccaccgcc agaagaacat
     4261 ccgggtcacc ggcgaaagcc cgcagcgcga gggcgagatc gtcctggaag aagcggaggc
     4321 gcacctggat gatcgcccct tccacgccca tccggccgta cgtcacgtgg aattcgtggg
     4381 cggaggcagc ctcggaatcc ggcacggcgg cggccggggg atccggcgct gcggcggccg
     4441 cgggttcgga cgtgacgccg gcccggggat ccggagcggc cacggccggg ggctgcgcgg
     4501 tcgcagcccc cagcccgacc gcgaacagcc aggcgactga ccgaatagac atcagccgtt
     4561 cccctgctcg cccgtttcgc gcaattccgg ggcgatccac gtgttgttgt ccgggttgat
     4621 gtccgcgtac gccgcatccg gatccaggac gaccctgttc accttgcggt ccgagaagaa
     4681 gcccttcgtg tactcgagct cgttgttccg ccatacgtcg gccggcattt ccatgcgttc
     4741 cgtcgagccg tcgtcgtagg tgacctccag ttcgagcggc atcaggaggc cgccctcgtt
     4801 tgacaccttt acccggtagt agtacgcccc ccgttccgag gagcccagga gcgagtcggc
     4861 cggctgcgaa gagacggact cgatcgcctg gtcgttcgcg tgcgtcgtgt agaaccagcc
     4921 gcgccagaac caggcgaggt tctcgcccgc tccctcgtcc atcgaccgga agaagtcggc
     4981 cggcatcggg tgccggaagg cccagccctg ggagtagccc gagaaggcct ggtcgaaggc
     5041 ctccgggccc aggatgtgct cgcgcagcat caccagtccc gtcgccggct tcgcgtaccc
     5101 gttgttgccg aaatccttgt ggatgaggtc ggactcggtc atgatcggca cctggtccgg
     5161 gtcgcgcatg taatccacga tgttctcggc cgggccccgg ctggacgggt acgtgcagtc
     5221 gtcggtctgc gtgaagtagg tgccgcagta cgtccgggcg tagtcgatct cgccgtagta
     5281 ctgcaggaac gtgttcagcc cctcgtccat ccaggtccac ttgcgctcgt cggacgcgac
     5341 gatcatggga aaccagttgt gcccgacctc gtggatggtg acgccgatga gcgcccgctc
     5401 cacctggtcg gagtacgtcc cgtcggccgc cgggcgcgcg ccgcagaagg cgaccatcgg
     5461 atattccatg ccgccgaccg ggccgttcac gttgatggcc ttctcgtagg gatactcgaa
     5521 cgccatccgg ccgtacgtct tcatcgtctg gatggtcgcc ctcgtggaga ccttgtccca
     5581 gagcggcatg gcctccttcg ggtacaggga atggatcagg atcgggtcgt ccgaggcgcg
     5641 gtagcggaaa ccggcggcat cccagacgaa ggccctggac gaggcgaacg cgaagtcgcg
     5701 cacgttattc gccttgaagt gccaggtgac cgttccggac gtgacagggc gcgcgaccgg
     5761 atcgccgacc tcgtccgccg ggacgatgaa gaccggctcc tcggacgtgt acgcccgctc
     5821 gagccgctcg cgctgggtcg cggtcagcac ctcctccggg ttctgcagcg tgccggtcgc
     5881 atcgaggatg tggttccacg gcaccgtca
//
LOCUS       DBNHQZ010000100         6170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3007370, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000100 DBNHQZ010000000
VERSION     DBNHQZ010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3007370"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC16YU_02525"
     CDS             <1..493
                     /locus_tag="AC16YU_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6064124.1"
                     /translation="TVGAIHGGVRSNIIPEEVELVGTIRTFEDDMRDTVHRRIREIAE
                     HTAAANGATVTVEIPMSASYPVTFNDPDLTAAMLPSLVRAAGEGNVELVDAETGAEDF
                     SFFANEVPGFYFNVGGMPRGMSRDEAPSHHTPDFFIDESGMRLGVQAMLNVALDYLNG
                     AAQ"
     gene            480..2741
                     /locus_tag="AC16YU_02530"
     CDS             480..2741
                     /locus_tag="AC16YU_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR6064125.1"
                     /translation="MPLSRSLAPARLAVALLLAWTSTAAAQTVPRVDGAEGDLHRLTA
                     PIRFDGRVDDDEWEAVPVLPLIQFWPTWGDPVVHPTEIRLAYDDEYLYLAARCFDTRP
                     PTMTTYRRDAWDDRDDQVAIGIDSFNDFENQLVFSTYATGARIDSQFSDDAREGFDPN
                     TDWNTYWDAETTVHEAGWDLEVRIPFSSLRFETSTDGPVTMGINVFRYRPHEGYLYHY
                     PGPPNDWGYWSFLKPSKGARRTLHGIESSKPLYVSPYVTSGLGQNWALDDAGTAYRRR
                     DEADFDAGLDLKYSLTSNLTLDLTANTDFAQVEADDQQVNLTRFSLFFPEKRQFFLER
                     TSNFEFSFGGSDRLFYSRRIGVAEGQEVTLLGGGRVVGRVGDWDVGLLNLQTGRTTVD
                     TDDGPLRLGTENFGVVRLKRRVLNENSWLGGIGTSRIGDGGDRNVAYGLDGVVNVTGD
                     EYLRFAWAQTFDDGRQDALAALDNSRFRLGWERSRSIGFIYGTSVSRSGYGYRPGIGF
                     QRREDYTQLEGEVGYGRLSGSASAIRGWSVEAEVESWYENRREAFGTLNAGAGGEITF
                     RSGASVRGNLNLEWEDLAEGFSLSDNAEVPAGEYRFVGGEVGGESPAGNPLYLSGELR
                     FGGFFDGRQVVAVLSPKWSATERISVTGTYQYSRITLPERDQVFEAHLVRGRIEWTLN
                     TRITLSTLVQYSGAADAAVLNFRFRYNPRDGNDLYLVYNEGFNTDRHAEDPTLPFTSG
                     RTVIAKYVHTFRW"
     gene            complement(2769..3551)
                     /locus_tag="AC16YU_02535"
     CDS             complement(2769..3551)
                     /locus_tag="AC16YU_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6064126.1"
                     /translation="MSRSFLIGNFLRAYGQSFGRLRRPVRTDGGLSTERLAVRFDERA
                     RRHGERGRVYVRNLQAGFVAALLLVTGSFRIDLRPAGDMDFAVEDQEVVVMEEIAQTR
                     QIQRPPPPPRPPVPVEVPNDAILDDVELDLDVSLDVELAVADLPPPPPDPVTAEEPEE
                     PEIFYVVEQMPEMIGGMAALVASLEYPELARKAEVEGMVVVQIVIPPSGIPQDPRIVR
                     SVGRVLDNAAVDAVMKQTFTPGRQRGKAVTVAMNIPVVFKLS"
     gene            3715..4959
                     /locus_tag="AC16YU_02540"
     CDS             3715..4959
                     /locus_tag="AC16YU_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6064127.1"
                     /translation="MSRSSVSTRLSVMMFLQFFIWGAWYVTIGNYMTAIGMTDLTHWP
                     YTVNPLAAIVSPFFLGLVADRYFSTERVLAVLHILGGLVMFAVPQFSGNATVFILLLA
                     LYNLCYMPTLGLTNSLAFHHIDDQEKQFPLIRVFGTIGWIVAGLFISFVLGRMAGEGI
                     KPDETALPLYGTAVASLVLGVFALFLPHTPPPARGQAVSVRSILGVDAFNALKSRPFM
                     IFLVSSFLICIPLAAYYNFAPIFVNAAGVENVAATMSLGQMSEVVFMLLMPLFFVRLG
                     VKWMLVVGMGAWVLRYALFAVAAPAGIFWMIAAGILLHGICYDFFFVTGQIYVDKKST
                     AAIRGQAQGMLVLVTYGLGMFIGAQIAGNVFNAFLGGASGLTPELWQQFWFIPAGFAA
                     LVMVFFALSFKDKVVPDPASHA"
     gene            4952..5911
                     /locus_tag="AC16YU_02545"
     CDS             4952..5911
                     /locus_tag="AC16YU_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6064128.1"
                     /translation="MHRRTFMRRTAAIAAGAGLYGLGCRPSGQGEPVATAAADPIFRI
                     SLAEWSLHRAIRAGEFDPLDFPVVSKRDYGIDAVEYVSALFTDGRSDTEYIGALRERC
                     DGEGVTSVLIMVDGEGALGDADEAARRQAVLNHHRWVGAAAALGCHSIRVNAQSTGSR
                     EEQAGRAADGLRRLCEFGDTMGINVIVENHGGFSSDGSWLADVMRRVDHPRVGTLPDL
                     GNFAVDEETFYDPYQGVAELMPYAKGVSAKAHNFSDAGGSVNSRFTDPFDYTRLMRIV
                     LDAGYRGFVGIEYEGSQLSEADGIRATKALLEQIRETLTPEYR"
     gene            5917..>6170
                     /locus_tag="AC16YU_02550"
     CDS             5917..>6170
                     /locus_tag="AC16YU_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR6064129.1"
                     /translation="MRFPSVLLLASALLAAACGGQPEPAPVDEAAAPGEWEDLFDGHG
                     FEHWRGYGQDSMPDSWAIIEGAMYNAVPGTDLVTRAQYAD"
BASE COUNT          960 a         2150 c         2039 g         1021 t
ORIGIN      
        1 cacggtcgga gcgatccacg ggggtgtccg ctcgaacatc atccccgagg aggttgaact
       61 ggtcggtacc atccggacgt tcgaggacga catgcgcgac acggtgcacc ggcgcatccg
      121 cgagatcgcc gagcacacgg cggccgcgaa cggcgcgacc gtcacggtcg agatcccgat
      181 gtcggcctcg taccccgtga ctttcaacga cccggatctc acggccgcga tgctgccgtc
      241 gctcgtccgg gccgccggcg agggcaacgt ggagctcgta gatgccgaaa cgggcgcgga
      301 ggacttctcg ttcttcgcca acgaggtccc ggggttctac ttcaacgtcg gcggcatgcc
      361 ccggggcatg tcgcgggacg aggcgccgtc acaccacacg cccgacttct tcatcgacga
      421 gagcggcatg cgcctgggcg tccaggcgat gctcaacgtc gcgctcgact acctgaacgg
      481 tgccgctcag tagatcgctc gcccccgccc gcctggccgt cgccctgctc ctggcatgga
      541 cgtccacggc ggccgcgcag accgtgcccc gagtggacgg ggccgaggga gacctgcatc
      601 ggctcaccgc tccgatccgg ttcgacggca gggtggacga cgacgagtgg gaagccgtgc
      661 cggtccttcc cctcatccag ttctggccca cctgggggga tcccgtcgtc cacccgaccg
      721 agatccgcct cgcctacgac gacgagtacc tctatctcgc ggcccgctgc ttcgacacga
      781 ggccaccgac catgaccacg taccgtcgcg acgcgtggga cgaccgcgac gaccaggtgg
      841 ctatcgggat cgattcgttc aacgacttcg agaaccagct ggtcttctcg acctacgcca
      901 ccggggcgcg catcgattcg cagttctcgg acgatgcgcg ggagggcttc gaccccaaca
      961 cggactggaa cacgtactgg gacgcggaga cgacggtaca cgaggcgggc tgggacctcg
     1021 aggtgcgcat tcccttctcg agcctccgct tcgagacctc gaccgacggc cctgtgacga
     1081 tgggcatcaa cgtcttccgg taccggcccc acgagggcta tctctaccac tatccggggc
     1141 ccccgaacga ctgggggtac tggagcttcc tcaagccgtc gaagggcgcg cggcgcacgc
     1201 tgcacggcat cgaatcctcg aagccgctct acgtctcccc gtacgtgacg tcgggtctgg
     1261 gacagaactg ggcgctcgac gacgccggaa cggcctaccg acgcagggac gaggccgatt
     1321 tcgacgctgg actggacctc aagtattcgc tcacgtcgaa cctgaccctg gacctcacgg
     1381 cgaacacgga cttcgcgcag gtcgaggcgg acgaccagca ggtgaacctg acccgcttct
     1441 cgctcttctt cccggagaag cggcagttct tcctggagcg gacgagcaac ttcgaattct
     1501 cgttcggcgg atcggatcgc cttttctaca gccggcgcat cggggtggcc gaggggcagg
     1561 aagtcacgct cctgggcggg ggccgggtgg tgggacgggt cggtgactgg gacgtgggcc
     1621 tgctgaacct gcagaccggc cggacgaccg tcgacaccga cgacgggccc ctgcggcttg
     1681 gcaccgagaa cttcggcgtg gtgcgcctga agcgacgggt cctgaacgag aactcctggc
     1741 tcggcggcat cggcacctcc cggatcgggg acggcggcga tcgcaacgtc gcctacggcc
     1801 tggacggcgt ggtgaacgtc acgggagacg aatacctgcg cttcgcctgg gcgcagacct
     1861 tcgacgacgg ccggcaggac gccctcgccg cgctcgacaa cagccggttc cggctcggat
     1921 gggagcgctc acggagcatc gggttcatct acggaacgag cgtctcgcgg tccggctacg
     1981 gctatcgccc gggcatcggc ttccagcgcc gggaggacta cacccagctc gagggtgagg
     2041 tcggatacgg ccggctctcc ggctccgcgt ccgcgatccg cggctggtcg gtcgaggcgg
     2101 aggtcgaatc gtggtacgag aaccgccggg aagcgttcgg cacgctgaat gcgggcgccg
     2161 ggggcgagat caccttccgg tcaggggcct ccgtgcgtgg caatctgaac ctggaatggg
     2221 aggacctggc ggagggcttc tcgctttcgg acaatgccga ggtgcccgcc ggcgagtaca
     2281 ggttcgtcgg gggggaggtg ggcggcgagt cgccggccgg caaccccctc tacctgagcg
     2341 gagagcttcg cttcggaggg tttttcgacg gaaggcaggt cgtggccgtc ctttcgccca
     2401 agtggagcgc gaccgaacgc atctcggtca ccggcacgta ccagtactcc cggatcaccc
     2461 tgccggaacg cgaccaggtg ttcgaggcgc acctggtccg cgggcggatc gagtggacgc
     2521 tcaacacccg catcacgctg tccaccctcg tgcagtactc gggagccgcc gacgcggcgg
     2581 tcctcaattt ccgcttccgg tacaatccgc gcgacggcaa cgacctctac ctcgtgtaca
     2641 acgagggctt caacacggat cgccacgccg aggacccgac gctgcccttc acgtccggcc
     2701 ggacggtcat cgcgaagtac gtccacacgt tccgctggtg atcccggccg gcccggcggg
     2761 acgtgggatc acgacagctt gaataccacc gggatgttca tggccaccgt aaccgccttc
     2821 ccgcgctggc ggcccggcgt gaacgtctgc ttcatgacgg catccacggc cgcgttgtcc
     2881 aggacgcgtc cgacggagcg gacgattctc gggtcctggg ggatgcccga gggcggaatc
     2941 acgatctgga ccaccaccat gccctcgacc tcggccttgc gggccagctc cgggtactcc
     3001 agcgaggcga cgagcgccgc catgccgccg atcatctcgg gcatctgctc caccacgtag
     3061 aagatctccg gctcctcggg ctcctcggcc gtcaccgggt ccggcggcgg gggcggaagg
     3121 tccgccacgg ccagctcgac gtcgaggctg acgtcgagat ccagttccac gtcgtccagg
     3181 atcgcatcgt tcggaacctc gaccggcacg ggcggcctcg gcgggggggg cggccgctgt
     3241 atctgccggg tttgcgcgat ctcctccatg accaccactt cctggtcctc gaccgcgaaa
     3301 tccatgtccc cggccggtcg gagatcgatg cggaagctcc cggtcaccag gaggagggcc
     3361 gccacgaagc cggcctgcag gttccggacg tagaccctgc cacgctcgcc gtggcgccgg
     3421 gccctttcgt cgaaccggac ggccaggcgc tccgtcgaca ggccaccgtc ggtccggacc
     3481 ggcctccgca gccggccgaa cgactgcccg taggcgcgca ggaaattccc gatgagaaac
     3541 gatcgtgaca tccgttcgcc cgtccgattt gatccggggt acggaaatcc cctacgcggg
     3601 ttacgcggcg cgggcgaagt ttccgtcaag cgggccgccc gcggggtgcg cgccgggttg
     3661 agattccgcc cgtccggcac taaatacggc gggtccaccc gcccgagacc gagcatgagc
     3721 agatcctccg tgagcacccg cctgagcgtg atgatgttcc tccagttctt catctggggg
     3781 gcgtggtacg tcacgatcgg caattacatg acggcgatcg ggatgaccga tctcacccac
     3841 tggccctaca cggtcaatcc gctcgccgcg atcgtctcgc cgttcttcct ggggctcgtc
     3901 gccgaccgct acttctcgac cgagcgggtg cttgcggtcc tgcacatcct cgggggactc
     3961 gtgatgttcg ccgtgccgca gttcagcggc aatgccacgg tgttcatcct gctcctggcg
     4021 ctttacaacc tctgctacat gcccacgctc gggctgacga actcgctcgc gtttcaccac
     4081 atcgacgacc aggagaagca gttccccctc atccgggtgt tcggcacgat cgggtggatc
     4141 gtggccggcc tcttcatcag cttcgtgctc ggccgcatgg cgggcgaggg catcaagccc
     4201 gacgagacgg ccctcccgct ctacggcacc gcggtagcca gcctggtact gggcgtcttc
     4261 gcgctgttcc tgccccacac gccgccgccg gcgcgcggcc aggccgtgtc cgtccggtcg
     4321 atcctgggcg tggacgcttt caacgcgctg aagagccggc ccttcatgat cttcctcgtc
     4381 agctcgttcc tcatctgcat cccgctggcc gcctactaca acttcgcgcc catcttcgta
     4441 aacgcggccg gggtcgagaa cgtggccgcc acgatgtcgc tcggccagat gtccgaggtc
     4501 gtgttcatgc tcctgatgcc gctcttcttc gtgaggctgg gcgtgaagtg gatgctggtc
     4561 gttggcatgg gagcctgggt cctccggtac gcgctcttcg cggtcgcggc cccggcgggc
     4621 atcttctgga tgatcgccgc cggcatcctc ctgcacggca tctgctacga cttcttcttc
     4681 gtgacgggcc agatctacgt cgacaagaag tcgaccgcgg ccatccgggg ccaggcgcag
     4741 ggcatgctcg tcctggtgac gtacggcctg ggcatgttca tcggcgccca gatcgccggc
     4801 aacgtgttca acgccttcct gggcggcgcc tccggcctca cgccggagct ctggcagcag
     4861 ttctggttca tcccggccgg cttcgccgcg ctcgtgatgg tgttcttcgc actctccttc
     4921 aaggacaagg tcgttcccga ccccgccagc catgcataga cgaaccttca tgcggcgcac
     4981 ggccgccatc gccgccggcg ccggactcta cggactcggc tgccgcccgt cggggcaggg
     5041 cgagccggtg gccacggccg ccgcggaccc gatcttccgc atttcgctcg ccgagtggtc
     5101 gcttcaccgc gccatccggg ccggtgagtt cgatccgctc gacttccccg tggtgtcgaa
     5161 gcgcgattac ggcatcgacg ccgtcgagta cgtgagcgct ctcttcactg acggccgctc
     5221 cgacaccgag tacatcggcg cgctccggga gcggtgcgac ggcgagggag tgaccagcgt
     5281 cctgatcatg gtcgacggcg agggagcgct cggcgacgcc gacgaggcgg cccggcgaca
     5341 ggccgttctg aatcaccacc ggtgggtcgg ggcggccgcc gcactcggct gccactcgat
     5401 ccgcgtaaac gcgcagagca cgggctcgcg cgaggaacag gccggtcggg ccgccgacgg
     5461 cctgcgtcgc ctgtgcgagt tcggcgacac gatgggcatc aacgtgatcg tcgagaacca
     5521 cggcggattc tcgagcgacg gcagctggct cgccgacgtg atgcggcgcg tggaccatcc
     5581 gcgcgtcggc acgctcccgg acctcggcaa cttcgcggtg gacgaggaga cgttctacga
     5641 tccctaccag ggcgtcgcgg agctcatgcc gtacgcgaag ggcgtgagcg cgaaggcgca
     5701 caacttctcg gacgccggcg gctcggtgaa ctcgcggttc accgacccat tcgactacac
     5761 gcgcctgatg cggatcgtgc tggacgccgg ctaccgcgga ttcgtcggca tcgagtacga
     5821 gggcagccag ctctccgagg ccgacggcat ccgcgcgacg aaggctctcc tggaacagat
     5881 ccgcgagacg ctcacccccg aataccggtg atcaacatgc gattcccttc cgtcctgctc
     5941 ctcgcttcgg cgctcctcgc ggccgcctgc ggaggccagc ccgagcccgc ccccgtggac
     6001 gaggccgccg cgcccggcga atgggaggac ctcttcgacg gccacggctt cgagcactgg
     6061 cggggctacg gccaggactc catgccggat tcctgggcca tcatcgaggg tgccatgtac
     6121 aacgcggtgc cggggacgga cctcgtcacc cgcgcgcagt atgccgattt
//
LOCUS       DBNHQZ010000101         2537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3279388, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000101 DBNHQZ010000000
VERSION     DBNHQZ010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3279388"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="AC16YU_02555"
     CDS             complement(<1..674)
                     /locus_tag="AC16YU_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6064130.1"
                     /translation="MRLVFRSLLLAALTLAAACSGPAEEPPAPAAPGTPVDGSSTRQI
                     RVQSVVLQPTGFEDVIQQTGAVEANDDATLSAQSGGTIETLVPLGSRVGKGDVLARLD
                     SELVLATLEQARAQLEVARAQYALADGLFRRQEPLYRDSIISDLEFDNVRSQRIQAQA
                     EVSRTEATVRQVEKQLENTRITAPFGGTVEQHFVEEGEQVLPGSPVLRLVNTRRIRIA
                     AGVPER"
     gene            complement(694..2274)
                     /locus_tag="AC16YU_02560"
     CDS             complement(694..2274)
                     /locus_tag="AC16YU_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6064131.1"
                     /translation="MRSVLPIASVLLALFSWCPGTPAAGQAPAPRPVSLTLDEAIQVA
                     LVSNHAVRSLRLDIDDARSRIREGWGELMPQVDLQSSFTRNIESVNPFSGSSAGGLFN
                     SLGFVDWLAYNEKARTDADPGTDPITVAEYFLRRQAGLDAAGVPAADPDENPFRIPST
                     YRSGLTVTQKLFDGRAIFGASGAQRWLPPLNEAAVSREEQRLVDQVRRAFYGTMLADQ
                     RAVVVSQSVQRAEATRQEVSRQVSEGVAPVFQRLSAEVELANLETQLVQATNDAEAAL
                     DQLRLLLGMPADQPVRLVGSLEGGVTLTAPSDSADAVVQALTRRPDLEQARISIELQK
                     VQLKVNRSAFLPELEAFANLSYIGNVPDNRLDVIPDPTDPFRFTTRTNGYFADPYWNW
                     EFNVGFQLRWTLFNGFRRHQQAQQTRIALEKARISRDFLEQSIATEVVRALRDVQAAR
                     RRMISQEQNVQRAALNYDHAEARLKEGVATPIEVREASSQLDQSRLNYLQAIHDYLVA
                     RSAYETAIGTPVSAATAP"
     gene            complement(2271..>2537)
                     /locus_tag="AC16YU_02565"
     CDS             complement(2271..>2537)
                     /locus_tag="AC16YU_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064132.1"
                     /translation="ARALAVPLRRAMERGEIRPLPPETVAHMLLGNIQGCQVLLSLGE
                     CGSMRGDAAPPWNASDAARFLTVMLFDGLLLPGPAVNSPSESAT"
BASE COUNT          372 a          810 c          950 g          405 t
ORIGIN      
        1 tagcgctccg gcacgccggc ggcgatccgg atgcgccggg tgttcacgag gcgcagaacg
       61 ggggatccgg ggaggacctg ctcgccctcc tcgacgaaat gctgctcgac cgttccgccg
      121 aagggcgccg tgatacgggt gttctcgagc tgcttctcca cctgtcggac ggtggcctcg
      181 gttctggaca cttcggcctg ggcctggatg cgctgcgacc gcacgttgtc gaactcgagg
      241 tcgctgatga tggaatcccg gtacagcggc tcctggcggc ggaaaagccc gtcggccagc
      301 gcgtactggg cacgtgccac ctcgagctgg gcgcgggcct gctcgagcgt cgcgaggacg
      361 agctcggagt ccaggcgcgc gaggacatcg cccttcccga cgcgcgagcc cagcgggacg
      421 agcgtctcga tggttccccc gctctgggcg gaaagggtgg cgtcatcgtt ggcctccacg
      481 gcgccggtct gctggatcac gtcctcgaag ccggtcggct gcaggaccac ggactgaacg
      541 cggatttgcc gggtggatga tccgtcgacg ggcgtgcccg gtgccgcggg tgccggcggc
      601 tcctcggcgg gcccggagca cgccgcggcc agggtcaggg cggcgaggag cagggagcga
      661 aagaccagtc gcatggtacg ggtcgttgaa tggttagggg gcggtcgcgg cggaaaccgg
      721 cgtaccgatg gcggtctcgt acgccgaacg ggcgacgagg tagtcgtgga tggcctgcag
      781 gtagttcagg cggctctggt ccagctggct ggatgcctcg cggacctcga tgggtgtcgc
      841 gacgccctcc ttgaggcggg cctcggcgtg gtcgtagttg agtgcggcgc gctgcacgtt
      901 ctgctcctgg ctgatcatcc gtcggcgggc ggcctggacg tcccgcaggg cccgcacgac
      961 ctcggtggct atcgactgtt ccaggaaatc ccggctgatg cgcgccttct cgagcgcgat
     1021 ccgggtctgc tgcgcctgct ggtggcgccg gaagccgttg aaaagcgtcc agcgcagctg
     1081 gaagccgacg ttgaattccc agttccagta gggatcggcg aagtacccgt tggtgcgggt
     1141 cgtgaaccgg aacgggtcgg tcgggtcggg aatgacgtcg agccggttgt cgggcacgtt
     1201 gccgatgtac gacaggttcg cgaacgcttc cagctccggg aggaacgcgc tccggttcac
     1261 cttgagctgc accttctgca gttcgatgct gatgcgggcc tgctccaggt ccggccttcg
     1321 ggtcagggcc tggaccacgg cgtcggcgct gtccgacggg gcggtaaggg taaccccgcc
     1381 ctccagcgag cctacgagac gtacgggctg gtcggccggc atgcccagga gcagtcgcag
     1441 ctggtcgagg gcggcctcgg cgtcgttggt cgcctggacc agctgcgtct ccaggttcgc
     1501 gagctccacc tcggccgaca gccgctggaa tacgggtgcg accccctcgg atacctggcg
     1561 gctcacctcc tgcctggtcg cctcggcccg ctgcacgctc tgcgagacga ccaccgcgcg
     1621 ctggtccgcg agcatggtgc cgtagaatgc gcggcggacc tggtcgacga gccgctgctc
     1681 ctcccggctg acggccgcct cgttcagggg gggcagccag cgctgggcac cggacgcgcc
     1741 gaagatcgcc cgcccgtcga agagcttctg agtgaccgtg agaccgctgc gatacgtgct
     1801 cggaatccgg aacgggttct cgtcgggatc ggcggcgggc actccggcgg cgtcgagtcc
     1861 cgcctgccgg cgcaggaagt actcggccac cgtgatcggg tcggttccgg ggtcggcgtc
     1921 cgtgcgggcc ttctcgttgt acgccagcca gtcgacgaat ccgagggaat tgaagaggcc
     1981 gccggccgag ctgcccgaaa acgggttcac ggactcgatg ttgcgggtga aggacgactg
     2041 caggtccacc tggggcatga gctctcccca tccctcgcgg atccgcgacc gggcatcgtc
     2101 gatgtccagc cgcaggctgc ggacggcatg gttgctgacg agggccacct ggatcgcctc
     2161 gtcgagcgtc agcgagaccg gtcggggagc cggcgcctgc ccggccgccg gcgtgcccgg
     2221 gcaccaggag aacagggcga gaaggaccga tgcgatcgga agaacggatc tcatgttgcg
     2281 gattcggagg gcgaattcac ggccggtccc ggaaggagca ggccatcgaa gagcatcacg
     2341 gtgaggaagc gggcggcatc ggacgcattc cagggagggg cggcgtcgcc gcgcatggat
     2401 ccgcactctc cgagggaaag aaggacctgg cagccctgga tgttgcccag cagcatgtgg
     2461 gccacggttt ccggcggcag tggccggatc tcgccccgtt ccatggcccg gcggagcggg
     2521 acggcgagag cccgggc
//
LOCUS       DBNHQZ010000102         4927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3143639, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000102 DBNHQZ010000000
VERSION     DBNHQZ010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3143639"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="AC16YU_02570"
     CDS             complement(<1..550)
                     /locus_tag="AC16YU_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013930.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR6064133.1"
                     /translation="MRLPDEIIEEVRAVADIVDVVGDYVRLKKRGSNFIGLCPFHAEK
                     TPSFNVNPRLGIFKCFGCGEGGDVFSFVARVENLPFPETVRLLAERFGVSIPEPEPED
                     GSGSEQESILHALRFAARFFHDRLARSDDADAARRYLQRRGLGTEVVKRFGLGYAPDA
                     WDTLLEAAREAGIAEETLEKAGL"
     gene            complement(567..704)
                     /locus_tag="AC16YU_02575"
     CDS             complement(567..704)
                     /locus_tag="AC16YU_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064134.1"
                     /translation="MSRLLLLLFVCCLAIPACQCSDKPEIGPVEGGEAALSASRPVNR
                     A"
     gene            854..2332
                     /locus_tag="AC16YU_02580"
     CDS             854..2332
                     /locus_tag="AC16YU_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6064135.1"
                     /translation="MFLTLILVAAGFLVVGRVASQDEKRPQELLASIVLLGLALALHL
                     ASGESLWVTAGRIALEFGIGLVLAAGLIARRRGPARPFLVLGFALLAVAVVVFGGRRL
                     LGVDGGPVSLLVELGPDDSIDELAPVLEAHDALAEPAFPRIELPEDEDLAQVFIVTVP
                     ARHEEPLRRALGLDRENVDHVEENRLIGLGPPIAAGTPAALTHPVLENDPLVGEQWAL
                     DAIRAHEAHRLLLGITPVRRARVAIVDTGVDAGHEDISAGMAGGPAGTDQHGHGSHCA
                     GIAGGVTNNGLGIASLNWEGRFVEVTAYRALDANGFGTIEQIAQGIIDAARDGADVVS
                     LSLGDRSRSAPKVLSDAIAYARRRGAVVVAAAGNADADAAMHTPSNVEGVIAVAALDP
                     SLRKARFSNVNTSLARPIAAPGVDILSLRTGGGYVPMSGTSMATPMVAGLVGVMRSIR
                     PELTGDEVYRILSETARDVPDAARTGRMIDAEAALRRVVDAG"
     gene            2322..2837
                     /locus_tag="AC16YU_02585"
     CDS             2322..2837
                     /locus_tag="AC16YU_02585"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6064136.1"
                     /translation="MPDDASPARVRAASPGDAARIASIYSESAAARDSTMDTGPVDGS
                     DVIGWLERLSPFETILVVEERGEVLGWGIAKRYSDRPGYARAAETSVYLDRAHVGRGL
                     GPMLQEELVAWCRRAGFHHLVAKIWAENAPSLAMHRRFGYETVGVQREIGFVGGAWRD
                     VAILQRILDET"
     gene            2882..4363
                     /locus_tag="AC16YU_02590"
     CDS             2882..4363
                     /locus_tag="AC16YU_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6064137.1"
                     /translation="MDGRSAPGAGTTPARPDLEGAAPCTLVWYESDLRTADHPALTEA
                     AARQAPVIPVFAWDGGAGWADGAASRWWLHRSLTDLREGLQLLGLDLVIRRGDPVHVI
                     PDLARALGADRVTWNARPEPDRAARDRRVAEALRARSVDAVPVRGSLLHDPDSVRTTS
                     GGPYQVFTAFHARIAADEATSVPLPVPGIGPRHRPRTAPETLAPGDLGLLAPDAPSPG
                     GDWVPGEAGAWERLDRFADEAVTRYAGLRDLPGKGGTSRLSPHLHFGEITPAQILHRL
                     LLGHGSHPSVLPFQRQLAWREFAFHLLHHFPGTPGRPLRPAFEAFPWRDDPRGLERWQ
                     AGETGYPMVDAGMRELLHTGWMHNRARMVVASFLTKHLLVSWTRGAAWFRERLVAADL
                     ANNTLGWQWSAGCGADAQPYFRIFNPVLQGTRFDPDGDYVRRWVPELGRLPPAHVHAP
                     WKASAATLAKAGIVPGVTYPWPIVDHGEARRRALAAWDAVRSG"
     gene            4431..>4927
                     /locus_tag="AC16YU_02595"
     CDS             4431..>4927
                     /locus_tag="AC16YU_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6064138.1"
                     /translation="MPTDRPPAADLLEARYREVRGRIDALLEDEDDWVSAMATVACEL
                     HHAMPHFHWTGFYRVVAPGMLAVGPYQGGHGCLRISFDRGVCGAAARTRRTQVVPDVE
                     AFPGHIACSSGTRSEIVVPVAAPGGGIVAVLDVDSDWPDAFGEADRDALEALCADLGR
                     RFGASA"
BASE COUNT          646 a         1837 c         1759 g          685 t
ORIGIN      
        1 cgagcccggc cttctcgagc gtttcctcgg cgatgcccgc ctcccgggcc gcttccagca
       61 gggtatccca ggcgtcgggc gcatagccca ggccgaaccg cttcacgact tccgtcccga
      121 gaccccggcg ctgcaggtag cggcgggccg cgtcggcgtc gtcggagcgg gccagccggt
      181 catggaagaa gcgggccgcg aaccggagcg cgtgcaggat cgactcctgc tcgctccccg
      241 aaccgtcctc gggctccggc tccgggatcg acacgccgaa ccgctccgcg agcaggcgga
      301 ccgtctccgg gaagggcagg ttctccacgc gcgcgacgaa gctgaacacg tcgcccccct
      361 ccccgcaccc gaagcacttg aagatcccga gccgcgggtt cacgttgaag gacggggtct
      421 tctccgcgtg gaagggacag agcccgatga agttggaccc acgtttcttc agccggacgt
      481 agtctccgac cacgtccacg atgtccgcca ccgcgcgtac ttcttcgatg atctcgtcgg
      541 ggaggcgcat cggccggtga ggcgtgtcag gcccggttca ccggtcggga cgcggaaagc
      601 gcggcctctc ctccctcgac gggcccgatc tcgggcttgt ccgagcactg gcaggccgga
      661 atcgcgaggc agcacacgaa gaggagcagg agcagtcggg acatggcgga acccgttgat
      721 tgagacttca agctaccccc gcagggggct cgcgggcaat gcgtgggcgg cgggtcgggc
      781 atgaaaccgg ccgcgccgga caacgtagcg taccgttacc aacaccggtc ttccgtaccg
      841 ggggcgagca ctcatgtttc tgaccctgat cctggtcgcg gcgggattcc tcgtcgtcgg
      901 ccgcgtggcg tcgcaggacg agaaacgccc tcaggagctg ctcgcctcga tcgtcctcct
      961 gggcctcgcc ctcgccctgc acctcgcgtc gggcgagagc ctgtgggtca cggccggccg
     1021 gatcgcgctg gagttcggaa tcggcctggt cctggcggct ggcctcattg cgcgccgcag
     1081 gggtcccgcg cggcccttcc tggtgctcgg cttcgccctg ctcgccgtcg ccgtcgtggt
     1141 gttcggaggc cgccggctgc tcggcgtgga cggcggcccc gtttcgctcc tcgtcgagct
     1201 cggtccggac gactcgatcg acgagctcgc gcccgtcctg gaggcccacg acgccctggc
     1261 cgagcccgcc ttcccccgca tcgaactgcc ggaggacgag gacctcgccc aggtgttcat
     1321 cgtcaccgtg ccggcgaggc acgaggagcc cctgcgccgg gccctgggcc tggaccgcga
     1381 gaacgtggac cacgtggagg agaaccggct catcgggctc ggcccgccga tcgcggccgg
     1441 gactcccgcc gccctgaccc atcccgtcct cgagaacgat ccgctcgtgg gcgagcagtg
     1501 ggcgctcgac gccatccgtg cccacgaggc gcaccggctt ctactcggga tcactccggt
     1561 ccggcgcgcc cgggtggcca tcgtcgacac cggggtggac gccgggcacg aggacatctc
     1621 cgccggcatg gccggcgggc cggccggcac ggaccagcac ggccacgggt cgcactgtgc
     1681 gggcatcgcg ggcggcgtga cgaacaacgg gctcggcatc gcctcgctga actgggaggg
     1741 ccggttcgtc gaagtgacgg cctaccgcgc gctcgacgcg aacggcttcg gcacgatcga
     1801 gcagatcgcg cagggcatca tcgacgccgc ccgggacggc gcggacgtcg tctcgctgtc
     1861 gctcggcgac cgctcgcgct cggcgccgaa ggtgctctcc gatgccatcg cctacgcgcg
     1921 ccgccgcggg gccgtcgtgg tggcggccgc cgggaacgcc gacgccgacg cggccatgca
     1981 cacgccctcg aacgtggaag gggtgatcgc cgtcgcggcg ctcgacccct cgctgcggaa
     2041 ggcccggttc tcgaacgtga acacgtcgct cgcccggccg atcgccgcgc cgggagtcga
     2101 catcctgtcg cttcgaacgg gaggcggata cgtacccatg agcggcacgt cgatggccac
     2161 gcccatggtc gcgggcctcg tgggcgtgat gcgctccatc cggcccgagc tgaccgggga
     2221 cgaggtgtac cgcatcctct ccgaaaccgc gcgggacgtg cccgacgccg cccggacggg
     2281 gcggatgatc gacgccgagg ccgcgctccg ccgggtcgtc gatgccggat gacgcctccc
     2341 cggcccgtgt ccgagcggcc tcgccgggcg acgccgcccg catcgcgtcc atctactcgg
     2401 agtccgcggc cgcccgcgac agcacgatgg ataccggtcc cgtggacggg agcgacgtga
     2461 tcggctggct cgagcgcctc tcccccttcg agaccatcct cgtcgtcgag gaacggggcg
     2521 aggtgctcgg atggggcatc gcgaaacgct actcggaccg cccgggctac gcccgtgcgg
     2581 ccgagacgtc cgtgtacctg gaccgggcac acgtcgggcg cggtctcgga cccatgctcc
     2641 aggaagagct cgtcgcctgg tgccggcggg cgggcttcca ccacctggtg gcgaagatct
     2701 gggcggagaa tgcgcccagc ctcgcgatgc atcgccggtt cggctacgag accgtgggcg
     2761 tccagcgcga gatcggattc gtcgggggcg cgtggcgcga cgttgccatc ctgcagcgta
     2821 ttctggacga aacctgaggg gcccccgccc cgcgtcccga accacgagct gcaggaccga
     2881 tatggatggc aggagcgcac ccggggccgg caccacgccg gcgcggccgg acctggaggg
     2941 tgccgcgccg tgtacgctag tctggtacga atcggacctg cgcacggcgg accacccggc
     3001 cctgacggaa gcggcagcgc ggcaggcgcc cgtcatcccg gtgttcgcgt gggacggcgg
     3061 ggccggatgg gcggacggcg ccgcctcgcg ctggtggctg caccggagcc tgacggacct
     3121 tcgggaaggg ctgcagctgc tcgggctgga cctcgtcata cgccgggggg accccgtgca
     3181 tgttatcccg gacctcgcac gcgcgctcgg agccgaccgc gtgacctgga acgcccgccc
     3241 cgagccggat cgcgcggccc gggaccggcg ggtggccgag gccctgcggg cccggagcgt
     3301 cgatgccgtc ccggtccggg gaagcctgct ccacgatccg gattcggtcc ggacgacgtc
     3361 gggcggcccg taccaggtgt tcaccgcctt ccacgcccgg atcgcggcgg acgaggccac
     3421 gtcggtcccg cttcccgtgc ccggcatcgg cccgcggcac cgcccgcgca ccgcgcccga
     3481 gacgctcgcg cccggcgacc tcgggctcct cgcgccggac gcgccctcgc cgggcggcga
     3541 ctgggtgccg ggcgaggccg gggcttggga acgcctggac cggttcgcgg acgaggcggt
     3601 gaccaggtac gccggactgc gtgacctgcc gggcaagggc ggcacctcgc gcctctctcc
     3661 ccaccttcac ttcggcgaga tcacgcccgc gcagatcctc caccggctgc tcctcgggca
     3721 cggctcgcac ccgtcggtcc tgcccttcca gcgccagctg gcctggcgcg agttcgcctt
     3781 ccacctgctg caccacttcc ccgggacgcc cggacgcccg ctgcgcccgg cattcgaagc
     3841 gttcccgtgg cgggacgatc cccgggggct cgagcgctgg caggccggag agaccggcta
     3901 cccgatggtc gacgcgggaa tgcgcgagct cctgcacacg ggctggatgc acaaccgcgc
     3961 gcgcatggtc gtcgcctcct tcctcacgaa gcacctgctc gtttcctgga cacggggcgc
     4021 cgcctggttc cgggagcggc tcgtagccgc cgatctcgcc aacaacacgc tcggatggca
     4081 atggtcggcc gggtgcgggg ccgatgcgca gccgtatttc cggatcttca atcccgtgct
     4141 ccagggcacc cggttcgatc ccgacggcga ctacgtgcgc cggtgggtgc ccgagctcgg
     4201 gcgtctgccc ccggcccacg tgcacgcccc gtggaaggct tccgccgcga ccctggcaaa
     4261 ggccgggatc gtgcccggcg tcacgtatcc ctggccgatc gtggaccacg gcgaggcgcg
     4321 gcgccgggcc ctggccgcct gggacgcggt acgctccggc tgaggccccc gcgccggcag
     4381 cgaacccgtc ggcgcgcgcc cggttgaacg ggcacccgat acgcgtcccc atgccgaccg
     4441 accgcccccc cgccgccgac ctgctcgaag cgcgctaccg cgaggtgcgc ggccgcatcg
     4501 acgccctgct cgaggacgag gacgattggg tgagcgccat ggcgacggtg gcgtgcgaac
     4561 tgcatcacgc catgccccac ttccactgga cgggcttcta ccgcgtggtc gctccgggca
     4621 tgctcgcggt gggaccgtac cagggcggac acggctgcct gcggatctcc ttcgaccggg
     4681 gcgtgtgcgg cgccgcggcg cgcacccgcc ggacccaggt cgtacccgac gtcgaggcct
     4741 tcccgggcca catcgcctgc tcgtccggca cgcgctccga gatcgtggtc ccggtcgcgg
     4801 cccccggcgg cggaatcgtc gcggtgctgg acgtggactc ggactggccg gacgccttcg
     4861 gcgaggccga ccgcgacgcg ctcgaggccc tgtgcgcgga cctcggccgt cgcttcggcg
     4921 cgtcggc
//
LOCUS       DBNHQZ010000103         4092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3280161, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000103 DBNHQZ010000000
VERSION     DBNHQZ010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3280161"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..811)
                     /locus_tag="AC16YU_02600"
     CDS             complement(125..811)
                     /locus_tag="AC16YU_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HIR6064139.1"
                     /translation="MLTKLYRSPVGKKVVTGITGIALSLFVLVHMTGNLAFFADETGE
                     AYNRYSHFLTHQLGFLFYAIEIGLLLIFVLHFTIGIDIWLGKRKARPHDYETYRSAGG
                     ASRQTLSSRSMILTGVILLAFTAWHLLSFKYGTYYASDILVDGEPIRDIARLVREKFQ
                     SPLYGIGYPVVMLLMGFHLRHGVWSALQSLGAVRPSATPAVYGTGAVVGFAIAIGFLV
                     LPLAIFFQFV"
     gene            1544..3976
                     /locus_tag="AC16YU_02605"
     CDS             1544..3976
                     /locus_tag="AC16YU_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6064140.1"
                     /translation="MSKEIVINTDSQKTRIAIVENGALAELFIETEEYERTIGNIFLG
                     RIRRIMPSIQAAFVDIGQKQDAFLHYSDLADNLADWLSFVQEDSPQIGEFEPRHSQRT
                     KGRRRRRPQHERRRSPHAESDEEREAAVDEDKGHPRSDARARGRRRSAQRRRPAPGRR
                     ERRDDDRPEKPRNARPAGDALPSTLLKKDLPILVKISKEPIAAKGSRITTDISLAGRF
                     LVLVPMADYVAVSKKISSFKERRRLRALARMLVPEGFGVIVRTVADGQNAKALDTDLR
                     LLLDRWRKIEKRLGEHPKPPAVVHQDVNMVSSIIRDLFSDNYERILVDSPRLYKNVKS
                     YIQAVAPEMVNAVKLHEAEEPVFRAAGIDKKVAEAFEKRVNMPSGGYLFFEQTEAMHV
                     IDVNSGRAGRGMSQEDSSLKVNLEAARIIARQIRIRDLGGIIVVDFIDMRDERSRRKV
                     LEELKKEFRRDRAVTKLLPMSDFGLIQITRQRLRPSITTTFSELQDAMNGEEAVAEAE
                     AVREKAPERAALPRPVRSADAGDTLTPEQLVHALDEWIRAFRAAGRKDPLRLQVHPFV
                     AAYLNRRIPSQTTRWFVRFMQRVRIEAVPTIDPLAYRFLDGETGNDLTVLPLAAAPAA
                     RDEAERPARPEPERREESRSSGERQGARPQGDARHESERSGRQGRDGGRSSGRRPADS
                     GSGEGRGEARRGTSGRGDEAGGESRRPEQSDAQRKSSRRGGRSGGSGGRGRGGAAKEA
                     SAPSAEEPRAQEQETKGQNGEAAPQRRSGRRRGSRGGRGRSRGRSSGNGDAQVRERTG
                     DGPKTPPGPDAD"
BASE COUNT          712 a         1340 c         1455 g          585 t
ORIGIN      
        1 cttgatcgag ttcctgtagc tttcccactt gtcctggagt cgacctgcgg gaaccttggc
       61 gtctagtacg ggagccatga cggatgcgtc ttgagtgcgt gcgtcggcga tggacggcgg
      121 gccgtcagac gaactggaag aagatggcca gcggcagcac caggaagccg atggcgatcg
      181 cgaaaccgac gaccgcgccg gtcccgtaca ccgcgggtgt cgcggacggg cgaacggcgc
      241 ccagcgactg gagagcgctc cacacgccgt gccgaaggtg gaaccccatg agcagcatca
      301 ccaccggata cccgatgccg tagagcgggc tctggaactt ctccctgacg aggcgggcaa
      361 tgtcacggat gggttcgccg tccaccagga tgtcggaggc gtagtacgtg ccgtacttga
      421 aggacaggag gtgccacgcg gtgaaggcga gcaggatgac tcccgtcagg atcatgctgc
      481 gggagctcag ggtctggcgg ctggcgccgc cggccgagcg gtacgtctcg tagtcgtgcg
      541 gccgcgcctt gcgcttgccc agccagatgt cgattccgat cgtgaagtgc agcacgaaga
      601 tcagcagcag gccgatctcg atcgcgtaga acaggaagcc cagctggtgg gtcaggaaat
      661 gggaatagcg attgtacgct tcgccggtct cgtccgcgaa gaaggcgagg ttgccggtca
      721 tgtgcacgag cacgaaaagg ctcagtgcga ttccggtgat gccggtgacc actttctttc
      781 cgaccggact gcggtacagt ttggtcagca tggcgctgcg ttcgggctgt gcgacggggt
      841 cagttccctg gctttcggcc cgctcggcct gccggcaagc gccggatcca cctcccgggc
      901 cgttggaaag taacgttcac tttcggaggc ggaatccgga cggtgtgcag gtcggatgaa
      961 ttcctgccgg gtgcccgcgg gatcatttgg ggcctgtcga ggattaacgg agcgcgtcgg
     1021 agccgccccg ccattgcggc gccgttccga ccggaatcga gtttctgacc ggacccccgg
     1081 caccccgacg gggtgcatcc atgaacttcc cgaagcgcac cacggcgtct gccggcgacg
     1141 gagcgaccga tccggtctgg atcggccgtc tgctgtcggc catcgtgcgc aggatggggc
     1201 tcatccggtc ccgacgcctc gacctgtcca ctccgaccgg cgaaccgccc gtgacggggc
     1261 cggggcgccg atccggccat tcccggctgc agcgcccgac tccgtccccc ggggccacgg
     1321 gcatgcctgc cggacactcc catcccttca gcgcacggat tggaatccgg aggattcccg
     1381 tccatccgcc ccgccggctc gtcgctcagc gacacgcccg ccgacgggtc gcggagcccg
     1441 gccatcatgc gtacggtccg gtcgggccgc caggcgggtc gccgccgtgc ggcacgcgga
     1501 ctgcgcgggc ccacggacgg ctccgtgccg cttgtgtcat tccatgtcca aagaaatcgt
     1561 catcaacacc gacagccaga aaacccgtat cgccatcgtc gagaacgggg cactggctga
     1621 gctcttcatt gagaccgaag agtacgagcg gaccatcggc aacatcttcc tgggccggat
     1681 ccgccgcatc atgcccagca tccaggccgc cttcgtcgac atcggccaga aacaggatgc
     1741 ctttctccac tactccgacc tcgcggacaa tctcgccgac tggctgagtt tcgtacagga
     1801 agactcaccg cagatcggcg agttcgagcc caggcactcg cagcggacca agggtcgtcg
     1861 gagacgccgg ccacagcacg agcggcgccg ttccccgcat gccgagtcgg acgaggagcg
     1921 cgaggccgcc gtcgacgagg acaaggggca tccgcggtcg gatgcgcgcg cccgtggacg
     1981 caggcggtcc gcccagaggc ggcgccccgc gcccggccgc agggagcgca gggacgatga
     2041 ccggccggaa aagccccgga acgcccggcc cgccggcgat gccctgccgt cgacgctcct
     2101 gaagaaggac ctgccgatcc tcgtcaagat cagcaaggaa ccgatcgcgg cgaagggcag
     2161 ccggatcacg accgacatct cgctcgctgg acggttcctg gtcctggtgc cgatggccga
     2221 ctacgtggcg gtgtccaaga agatatcgtc gttcaaggag cgccgcaggc ttcgtgcgct
     2281 ggcccgcatg ctggtacccg agggattcgg cgtcatcgtg cgcacggtcg ccgacgggca
     2341 gaatgcaaag gccctggaca ccgacctgcg cctgctgctg gaccggtggc gcaagatcga
     2401 gaagcggctc ggggagcacc ccaagccgcc ggccgtcgtc caccaggacg tgaacatggt
     2461 ctcgtcgatc atccgggacc ttttttcaga caactatgag cggatcctgg tcgacagccc
     2521 gcgactgtac aagaacgtga agagttacat ccaggccgtg gccccggaaa tggtcaatgc
     2581 cgtgaagctg catgaagccg aggagccggt gttccgggct gcgggaatcg acaagaaggt
     2641 ggccgaggcc ttcgagaagc gggtgaacat gccctcgggc ggttacctgt tcttcgagca
     2701 gaccgaggcg atgcacgtga tcgacgtgaa ctcgggccgc gcgggaaggg ggatgtccca
     2761 ggaagacagc tcgctgaagg tgaacctcga ggcggcgcgc atcattgcgc ggcagatccg
     2821 gatccgtgac ctgggcggaa tcatcgtcgt ggacttcatc gacatgcgcg acgagcgctc
     2881 gcgccggaag gtgctcgagg agctgaagaa ggagttccgg cgggaccgcg ccgtcaccaa
     2941 gctcctgccg atgagtgatt tcggcctcat ccagatcacc cgccagcggc tccgtccgag
     3001 catcacgaca accttctcgg agctgcagga cgcgatgaat ggcgaggagg cggtggccga
     3061 ggccgaggcg gtgcgcgaga aggccccgga gcgggctgcg cttccgcgac ccgtgcggtc
     3121 tgcggacgcc ggagacacgc tgacgcccga gcagctcgtg cacgcgctcg acgaatggat
     3181 ccgggccttc cgagcggcgg gaaggaagga ccctctccgt ctgcaggtgc acccgttcgt
     3241 ggcggcctac ctgaaccggc ggattccgag ccagacgacg cgctggttcg tccggttcat
     3301 gcagcgggtc cgcatcgagg cggtgcccac gatcgatccc ctggcatatc gttttctgga
     3361 cggggagacc gggaacgacc tgacagtgct tcccctggcg gcagctcccg cggcccgcga
     3421 cgaagcggag cgccccgccc ggccggagcc ggagcgcagg gaggagtcac gctcatccgg
     3481 tgagcggcag ggcgcgcggc cgcagggcga tgcgcggcac gaatcggagc gatcgggccg
     3541 ccagggccgc gacggaggtc gttcgtccgg tcgcaggccg gccgattcgg gctcaggcga
     3601 gggcagggga gaggcgcggc gcggcacgtc cggtcgcggg gacgaggccg gaggcgagtc
     3661 ccgtcggccg gagcagagcg acgcccagcg gaagtcctcc cgcaggggcg gccgatcggg
     3721 cggatccggg ggacgtggcc gcggtggtgc ggcgaaagaa gcgtcggccc cctcggcgga
     3781 ggagccccga gcccaggaac aggagacgaa gggtcagaac ggagaagccg ccccccagcg
     3841 ccggagcgga cgccgacgcg gaagccgcgg tgggcgtggc cggtcgaggg gccgctcgtc
     3901 gggcaacggg gacgcccagg tgagggagcg cacgggggac ggcccgaaga cgccgcccgg
     3961 tccggacgca gactgaccac gggccgatcg cttcgatgtc cttgcgcctc gctctcgcga
     4021 cgctcgtcgc gctcgccctc ccgctcggag cggaggccca gccgggaccg cgtccggcag
     4081 caccgatgaa cg
//
LOCUS       DBNHQZ010000104         5288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3008677, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000104 DBNHQZ010000000
VERSION     DBNHQZ010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3008677"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC16YU_02610"
     CDS             <1..635
                     /locus_tag="AC16YU_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR6064141.1"
                     /translation="ARLGRFSPAQGPRSFFSEVNYRDAMVMRTHGYHWIDLARMAEEP
                     HPSPIRATPSLYNIWDSRSEGLATAMEEMMMHAGLFDASPRTRELIWILVAQRAARAI
                     AGLRLHSNEWSLDEAVEFAARWTPRGWMQATSDLVWFEQHLYLMQPGYGSSYLAGKSQ
                     IEELVAARQHQLGDAFTVRGFFDELNAAGLVPVSLIRWELTGDEGAFVSR"
     gene            759..2327
                     /locus_tag="AC16YU_02615"
     CDS             759..2327
                     /locus_tag="AC16YU_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064142.1"
                     /translation="MASRNLGAVALAWVLAVAFLASCRTLKRELIPTDQVVTGQGSAT
                     TRLQDAFAGEPFVKAHMADGSAYVLADGTFDQDAGVHTGSGVRHDPYRRTETVGDFRI
                     PIDSVVVFETNRMGTSGAASALTIVTGISLAVTGYCLANTKACFGSCPTFYGDTADGP
                     HLLAEGFSSSIAPSLERTDVDDLSGIAARDGTVTIEMRNEAFETHVVRSVRLLAVPSI
                     GDADVFAGPDGRFFVAARVTNPTGCADERGSCLGSTAARDGIEWFGAADSTDLAGTEI
                     VELDFGETDSSSRYGVVVSARQTLLSTFVLYQALAWMGTDIGTWFTRIENRDLAESPL
                     SLQMLFGAVEVMVPGADGEWSVAGAVTEVGPLATDTHVIPLPVGVSADRIRLRVRRGG
                     WRIDQVALATLSEAPAVISIEPASVSRDGLADPDWTAALLDPQRTLVTMPGDRYRIRF
                     RLPDPDAGYHVFLESRGYYLEWIRDEWMRETDPQQVVRLMLDPAAMLKAMAPAFKQKE
                     SSMESVFWSSRYAY"
     gene            2317..2853
                     /locus_tag="AC16YU_02620"
     CDS             2317..2853
                     /locus_tag="AC16YU_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064143.1"
                     /translation="MRTDPRVILLVFVLATSAGCASSGAPPGFLVPPADADAWVLGGW
                     VMITTGAEEPAPTGRIEGELLAAGEDSVFVLTSDGLLQGVGVREIRDAEVWGYKPDHS
                     ALGIWTFVGTLSTLSHGVAFVLSAPVWLLAGISATASRSREPRIRYPTRPISEFSMYA
                     RYPQGMPPGFPRSPDPAP"
     gene            2960..3115
                     /locus_tag="AC16YU_02625"
     CDS             2960..3115
                     /locus_tag="AC16YU_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064144.1"
                     /translation="MAKRPNFNFEKRQREQAKKKKKQEKLERKRARKSPDADSPPDTE
                     RQPDPDA"
     gene            3275..>5288
                     /locus_tag="AC16YU_02630"
     CDS             3275..>5288
                     /locus_tag="AC16YU_02630"
                     /inference="COORDINATES: protein motif:HMM:NF021942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR6064145.1"
                     /translation="MMRAFLRAALVAASAAFVFAASVSAQPVDLTTVRAGEYDNGRMW
                     TFDFPPVDELQAAYGFAPDEAWFEQARLGALRLPNCTASFVSPHGLVMTNHHCGRGAV
                     AQVAEPGETLLDDGFTTASLEEERAVPGLYVDQLVAIEDVTDEIYAALEGMETDAEKA
                     AARQEAIQAAQARVLEAAGGPDAGFVVQVINLYNGGRYSAYTFRRFTDLRLVMAPELN
                     LGYFGGDTDNFTYPRYALDMTFFRVYDEDGTPYEPEHYFKWSADGSREGEPVFVIGNP
                     GSTLRLETVAQLEWRRDVQEKTLLALLNTRIEALESYYEENPSDGLRNQIFGLKNAQK
                     LYTGRVKGLNDPVIMAKRADTERQFLEDLEAKYGDRISEDVDVPYLTVIDDLAAIQDQ
                     KRQYAAEYRAFLSMGSGSALASSTLGRAYAARAYLLQRAETGSENARLWGQVEDMPSQ
                     DPGLDRRYIAARIREFVAAFGETDPAVQEALGGSTPDEVAAAVMEGSALVSGESAAAA
                     LADGSLSLDDPAIRLVAPFAERIQAYQSAMSGLMGQEAELNAQHGRARFEVYGTTRPP
                     DATFSLRIADGVVSAYEYNGSRAPAYTTFYGLYNRHYGHASGDDPTGEWQLPERWLNA
                     PETFDRSTPLNFVSTNDIIGGNSGSPMLNRDLEVVGLIFDGNIESLP"
BASE COUNT          831 a         1828 c         1770 g          859 t
ORIGIN      
        1 aggcccgcct gggacgattc tcgccggccc agggtccccg gtccttcttc tccgaggtga
       61 actaccggga cgccatggtc atgcgcacgc acggctacca ctggatcgac ctcgcccgta
      121 tggccgagga gccgcacccg agcccgatcc gggccacgcc ctcgctctac aacatctggg
      181 acagccgcag cgagggcctg gcgaccgcca tggaggaaat gatgatgcac gccgggctct
      241 tcgacgcgag tccccgcacg cgcgagctca tctggatcct cgtcgcgcag cgcgcggccc
      301 gcgcgatcgc ggggctccgc ctgcactcga acgagtggtc gctcgacgag gcggtcgagt
      361 tcgcggcgcg gtggacgccc cgcgggtgga tgcaggccac gagcgacctc gtctggttcg
      421 agcagcacct gtatctcatg cagccgggct acgggtcgag ctacctcgcc gggaagtccc
      481 agatcgagga gctcgtcgcg gcccgccagc accagctcgg cgacgcgttc acggtccggg
      541 gcttcttcga cgagctgaac gccgcgggcc tggtcccggt gagcctcatc cggtgggagc
      601 tgaccgggga cgagggggcg ttcgtcagcc ggtaggcgcc gacgggcccc ctgcgccggg
      661 gagagactct cctgtccgcc cggaaccgaa tggttgcttt tccgctccgg acggcgtaat
      721 catcccggca ggattccttt cgcccgggtg ttccgctcat ggcttcccgg aatctcggcg
      781 cagtcgcgct ggcgtgggtt ctggccgtcg cattcctcgc ttcgtgtcgg acgctgaagc
      841 gggagttgat ccccacggac caggtcgtga ccggccaggg ctccgcgacg accaggctgc
      901 aggatgcttt cgccggggag ccgttcgtca aggcgcacat ggcggacggt tcggcatacg
      961 ttctcgcgga cgggaccttt gaccaggatg cgggcgtgca caccgggtcg ggcgttcgac
     1021 acgatccgta ccgccgcacc gagacggtgg gggacttccg gatcccgatc gactccgtgg
     1081 tggtcttcga gaccaacagg atggggacgt ccggtgcggc aagcgctctt acgatcgtga
     1141 cgggcatctc cctggccgtg accgggtact gcctcgcgaa caccaaggcc tgcttcggat
     1201 cctgcccgac cttctacggg gatacggcgg acggaccgca tctgctcgct gaaggcttct
     1261 cgtccagcat cgctccctcc ctggagcgga ccgacgtgga cgacctctcc gggatcgcgg
     1321 cgcgtgacgg aacggtaacg atcgagatgc gaaacgaggc cttcgagacg cacgtcgtcc
     1381 ggagcgtgcg cctgctcgcc gtcccttcca tcggcgacgc cgacgtgttt gcaggaccgg
     1441 acggccggtt ctttgtcgcg gcacgcgtga cgaatcccac ggggtgcgct gacgagcgcg
     1501 gctcgtgcct gggctcgacg gcggcgaggg acggcatcga atggttcggt gcggccgatt
     1561 cgaccgacct tgcggggacc gagatcgtcg agctggattt cggcgagact gattcgtcga
     1621 gccggtacgg ggtggtggtg tctgcccgcc agaccctcct gtccacgttc gtgctgtacc
     1681 aggctctcgc gtggatgggc acggatatcg gcacctggtt cacgcggatc gagaaccgcg
     1741 acctggccga gtcccccctg agccttcaga tgcttttcgg ggccgtcgag gtcatggtgc
     1801 ccggcgccga cggagaatgg tccgtcgcgg gcgcggtgac ggaggtcggg cccctcgcca
     1861 ccgacacgca tgtcattccg ctccctgtag gcgtctcggc agaccgaatc cggctgcgtg
     1921 tccggcgcgg tggatggaga atcgaccagg tcgcgctggc gaccctctcc gaagccccgg
     1981 ccgtgatatc catcgagccg gcgtcggtgt cgcgggacgg cctcgcggat ccggactgga
     2041 cggccgcgct cctggacccc cagcgcacgc tcgtaaccat gcccggagat cgataccgga
     2101 tccgcttccg cctgccggat ccggacgcgg gataccacgt cttcctggag agccggggct
     2161 attacctcga gtggatccgg gacgaatgga tgcgggagac cgatccgcag caggtggtgc
     2221 gactcatgct cgaccccgcc gcgatgttga aggccatggc tcccgccttc aagcagaagg
     2281 aatccagcat ggaatccgtc ttctggtcca gccggtatgc gtactgatcc gcgcgtcata
     2341 ctgctcgtgt tcgtgctggc gacttctgcc ggatgtgcat cgtcgggcgc gccgcccgga
     2401 ttcctcgtgc cacccgcgga tgcggatgcc tgggtccttg gcggctgggt catgatcacg
     2461 accggtgcag aggagcctgc accgaccggc cggatcgaag gcgagctcct ggccgccgga
     2521 gaagattcgg tgttcgtcct gacgtcggac ggcctcctcc agggcgtggg cgttcgggag
     2581 atccgggacg ccgaggtgtg gggatacaaa cccgaccact ccgccttggg gatatggacc
     2641 ttcgtcggaa cgctgtccac cctttcgcac ggggtcgcat tcgtcctgtc cgccccggtc
     2701 tggcttctcg cgggcatcag cgcaacggcc tcccgttcaa gggagcctcg catccggtac
     2761 ccgacgcggc cgatctcgga gttctccatg tatgcccggt acccccaggg catgccgccc
     2821 ggttttccgc ggtcgccgga ccctgcgccc tgaagcgccc ggccggcgat gacggtgggt
     2881 agccgatcgg gcgcggctgc cctaccttgg gccttcacac cgttcgccgc gattttcgct
     2941 gcaccccaac ccggatccca tggccaagcg ccccaatttc aacttcgaga aacggcaacg
     3001 cgagcaggca aagaagaaga agaagcagga aaaactggag cgcaagcgcg cgcgaaagtc
     3061 gcccgacgcc gattcgcctc ccgacaccga gcgccaaccg gatccggacg cctgacggcc
     3121 ggaaaccgcc gggccggtga cccgcgtaca tcccgcactt caggcgagcc ttccggcatt
     3181 cccttacacg caccgtaagg gatgtccggc ttcgcgccat gaccggattg cgtccattat
     3241 gcggttccga catttcaccc caccggtaat cccgatgatg cgtgcattcc ttcgcgcggc
     3301 cctcgtcgcg gcgtccgccg cgttcgtctt cgccgcgtcc gtttccgccc agccggtcga
     3361 cctgacgacg gtccgggccg gcgagtacga caacggccgg atgtggacct tcgacttccc
     3421 cccggtcgac gagctgcagg ctgcgtacgg cttcgcgccc gacgaggcct ggttcgaaca
     3481 ggcccgcctc ggcgccctgc gccttccgaa ctgcaccgcg tccttcgtct ccccccatgg
     3541 gctggtgatg accaaccacc actgcggccg cggggcggtg gcccaggtcg ccgagcccgg
     3601 tgagacgctg ctcgacgacg gcttcacgac ggcctcgctc gaggaggagc gtgcggttcc
     3661 cggcctgtac gtggaccagc tcgtcgcgat cgaggacgtg accgacgaga tctacgccgc
     3721 cctcgagggc atggagaccg acgccgagaa ggccgcggcc cgccaggagg ccatccaggc
     3781 cgcccaggcc cgcgtgctcg aggccgccgg agggccggac gcgggcttcg tggtccaggt
     3841 catcaacctg tacaacggag ggcgctactc cgcctacacg ttccgccgtt tcacggacct
     3901 gcgccttgtc atggcccccg agctgaacct cggctacttc ggcggggaca cggacaactt
     3961 cacctatccc cgctacgcgc tcgacatgac gttcttccgc gtctatgacg aggacgggac
     4021 gccctacgag ccggagcact acttcaagtg gagcgccgac ggcagccgcg agggcgagcc
     4081 cgtgttcgtg atcggcaacc caggatcgac actgcgcctg gagaccgtgg cgcagctcga
     4141 gtggcgccgc gacgtgcagg agaagaccct tctcgcgctg ctgaataccc gtatcgaggc
     4201 cctggagtcg tactacgagg agaatccgtc ggacggcctc cgcaaccaga ttttcggcct
     4261 gaagaacgcg cagaaactgt acaccggccg ggtgaagggc ctgaacgacc cggtgatcat
     4321 ggcgaagcgg gccgataccg agcggcagtt cctggaggac ctcgaggcga agtacggcga
     4381 ccggatctcc gaagacgtgg acgtcccgta cctgacggtg atcgacgacc ttgccgcgat
     4441 ccaggaccag aaaaggcagt acgcggccga gtaccgggcc ttcctgtcga tgggctcggg
     4501 ctccgcgctc gcctcgtcca ccctcggccg ggcttacgcg gcgcgggcat accttctgca
     4561 gcgcgcggag accgggagcg aaaacgcacg gctctggggc caggtggagg acatgccatc
     4621 gcaggatccc ggtctggacc ggcggtacat cgcggcgagg atccgtgagt tcgtcgccgc
     4681 attcggcgag accgaccccg ccgtgcagga agcgctgggc ggctcgacgc cggacgaggt
     4741 cgccgcggcc gtcatggagg gctcggcgct cgtgagcggc gagtcggcgg cagccgccct
     4801 cgccgacggc tcgctctcgc tcgacgatcc ggccatccgg ctcgtcgcac ccttcgcgga
     4861 acgcatccag gcctaccaga gcgcaatgtc cggcctgatg gggcaggagg cggagctgaa
     4921 cgcgcagcac ggccgggcgc gcttcgaggt gtacggcacg acccggccgc ctgacgccac
     4981 gttctcgctg cgcatcgcgg acggcgtcgt gagcgcctac gagtacaacg gctcccgcgc
     5041 gccggcctac acgacgttct acggactgta caacaggcat tacggccacg cctccggcga
     5101 cgacccgacc ggcgagtggc agcttccgga gcgctggctg aacgccccgg agaccttcga
     5161 ccgctccacc ccgctcaatt tcgtgtcgac caacgacatc atcggcggca actccggctc
     5221 gccgatgctg aaccgggatc tcgaggtcgt ggggctcatc ttcgacggca acatcgaatc
     5281 gctgccgt
//
LOCUS       DBNHQZ010000105         8027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1249671, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000105 DBNHQZ010000000
VERSION     DBNHQZ010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1249671"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="AC16YU_02635"
     CDS             <1..1890
                     /locus_tag="AC16YU_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6064146.1"
                     /translation="VKLLGSVGSFALLLLSIAVTGLLLVDRDIQRSLDRVETDFRRLG
                     AAVASGFRGWRTRRAERRELVARVRNERARAERQAAPRTAEILVTRIPEADRTSPPPP
                     RSTAPGTPDLTVNVVEPVREARADHVPRRPVIESDFEYEMPSLDLLDEDEGARARVDD
                     HEIEENKRILLDKLSTYNIEITAIDAIVGPTVTLYELTPAPGVKISRITALEHDLAMA
                     MAARALRMIAPIPGKTAIGVEIPNRHRELVRIRDVIGTQRFRDAHMELPLILGKSIEG
                     EVVLQDLTRLPHVLIAGATGSGKSVGLNTFIAGMLYACPPSHVKFVMIDPKKIELAAY
                     ARLEAHYLAMPPEADAAIITDFGQALGTLKSCEIEMDTRYDLLSEARVRSVKEYNDRF
                     DAGQLDPDEGHRRLPYIVVVVDELADLMMTAGKDIEAPIARLAQKARAVGLHLILATQ
                     RPSVDVITGLIKANFPSRIALQVASKIDSRTILDQNGAEGLVGNGDMLYMHGSRVTRL
                     QGPFVSIEEIERVTEFIGSQEGPGPYLLPSLEDGGGMGTDAGAGSSSERDELFEEAAR
                     IIVRSQQGSVSLLQRKLSVGYSRAARIVDQLEDAGIVGPFEGSKARQVRVADETELDN
                     LLAAD"
     gene            1921..2433
                     /gene="purE"
                     /locus_tag="AC16YU_02640"
     CDS             1921..2433
                     /gene="purE"
                     /locus_tag="AC16YU_02640"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR6064147.1"
                     /translation="MSDATPLVGITMGSESDWPIMREAADVLRRFGVPFEVRVLSAHR
                     TPDATQAWASGAAARGIKVLIAGAGGAAHLAGVVAAATHLPVIGIPVPSKHLQGLDSL
                     LSMVQMPRGVPVATVAVGGAANAGLLAVQILSTADAALADAMRAYKDEMLGKVDEMDR
                     RVRESLASDA"
     gene            complement(2438..4849)
                     /locus_tag="AC16YU_02645"
     CDS             complement(2438..4849)
                     /locus_tag="AC16YU_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064148.1"
                     /translation="MRRLLMLATLLAVPLMAGAQERQDPMTEGILAFREERYDAAVRA
                     FERALDADPANAEAHFLLARIFFETPLRDVGRAGSELDRALEIEPENVQYLVARMQQL
                     RAESWNFIVEKLRESQRLTLAHRILALDSTNAFAHEELGASYIRDFWRYRNAVMLPTM
                     LYRDYSYRGRTTSDPVAARLQDQLADISTNPDLDTEIAGDFAATFAGIYDAESVFLAD
                     EFDLEALESQGVLIQSLAGRAQRVYDRAVMHLKRALESDPRHRSVYDYLMEIFALKGE
                     WPDALAMLQQMYVFFPDDPELWTYLGLAHHRAGNLDAAAKAFESAFEFMDADTRTAYD
                     DLGIIVPEDEKAAYEADPAGYASRFWTSKDPRFLTPYNERKVEHYARITYADLLYGAP
                     DVNLRGWETERGRILIRYGPPLTDVVIVPKSSSGVAVRSEERLPTATDPSQVADPILA
                     VAKQGTGFDMFEEANTYNVWSYGDFKFVFEDPFRNGEYRMYSPPAAAIADGAQPWVND
                     YVIRARETFRDLPDRFEYEAPGREIELPFLVASFRGPGGVTDLLVNYAIPISEFAQPG
                     EVINVTASAGLFVVSPDRDILAERRRTLYGLRTDQVVQFREANLWVDSEELNVAPGRV
                     DVSVEFETTSGNTVGVQRRSVTLPDFSGTDLSVSDVLLAYRVEETPDGRPITGADIVR
                     RGLSIMPAPWSVFSHEQPIYLYFEVYNLEMGDGGRAEYEVEAALVPKEGGSGVSRFFR
                     GLFGGGRGGVSAGLPISVASTEDGQYLILDASNQEEGLYSLRLRVRDRVSGREAGREL
                     DLYLE"
     gene            complement(5111..5584)
                     /gene="smpB"
                     /locus_tag="AC16YU_02650"
     CDS             complement(5111..5584)
                     /gene="smpB"
                     /locus_tag="AC16YU_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6064149.1"
                     /translation="MSDEEQGIKTVVTNRKARHEYHIHETVEAGLVLTGTEVKSLRAG
                     RANLQDAYAVMQGGELMLMQCHISPYEFGNRENHDPLRPRKLLLNRREIEKWDRAVRQ
                     KGATIIPLKLYFRRGYAKVLLGLARGKRQYDKRVDIADRESKRKLDRVRKSGRDD"
     gene            complement(5599..6441)
                     /locus_tag="AC16YU_02655"
     CDS             complement(5599..6441)
                     /locus_tag="AC16YU_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HIR6064150.1"
                     /translation="MDTPEPCRLALWDDEALGAFAADLADACRRFGVTIERLPRRECE
                     RRLGAGLVEAALVPTVSVLSAPDAYDVYPTVAMSAWCTPHASIHLRDGLERQPGTLSC
                     AAGLGQERLMARIVLREHYGMQPAVVEAAPGDEPDAAAGSVLHVGPLLVGRDPTAPEE
                     AGITLDLGQEWFELTAYPMVWALIASRVGESDDRVSLALKEAFGPSGDESDVEEPITE
                     LVSDTTLRFRMDDLATASLTELAELLFFYKVTEDVPDLRFAPDPEAAPAPEPDDDDDA
                     EFRI"
     gene            complement(6530..6880)
                     /gene="rplS"
                     /locus_tag="AC16YU_02660"
     CDS             complement(6530..6880)
                     /gene="rplS"
                     /locus_tag="AC16YU_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6064151.1"
                     /translation="MATDLMTLVEGTQLREETPDFQPGDTVNVHVRVIEGDKERIQQY
                     QGVVIAEKGSGARRTFTVRKVSNGVGVERVFPLHSPKVARIEVVRRGRVRRAKLYYLR
                     ERKGKSARIRERTR"
     gene            complement(6905..7612)
                     /gene="trmD"
                     /locus_tag="AC16YU_02665"
     CDS             complement(6905..7612)
                     /gene="trmD"
                     /locus_tag="AC16YU_02665"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6064152.1"
                     /translation="MRIDLVTALPGIFESPLRQSIVRRAQDSGLVDIRVHDLREHAHD
                     RHRQVDDYPFGGGAGMVLKPEPLFECVEGLLADGERADEVIFLTPDGETLTQDLANEL
                     SLASRLVLIAGHYKGVDQRVRDALVTREVSIGDYVLSGGELPALVLVDAVVRLIPGAL
                     GDAESALSDSFQDGLLDAPVYTRPAEFRDMHVPDVLLSGDHARVEAWREAQRLDKTRL
                     RRPDLMKAWRAESSRTE"
     gene            complement(7617..7751)
                     /locus_tag="AC16YU_02670"
     CDS             complement(7617..7751)
                     /locus_tag="AC16YU_02670"
                     /inference="COORDINATES: protein motif:HMM:NF051323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064153.1"
                     /translation="MRRDTGGDALVPFVPALIESVNEVRIVVRDVEGLLDPDSQEEVA
                     "
     gene            complement(7688..>8027)
                     /locus_tag="AC16YU_02675"
     CDS             complement(7688..>8027)
                     /locus_tag="AC16YU_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6064154.1"
                     /translation="ADAAAYRVTGVRYQPMQSGMAVVLSLEGLASREAAEEVSGLTVW
                     AEEDALPPLEDDEVFVSDLVGLEVHDADGRRAGWSRTCSIFPRSPCSWCGGTPEATRS
                     CRSSPLSSRA"
BASE COUNT         1288 a         2842 c         2645 g         1252 t
ORIGIN      
        1 gtgaagcttc tcggttcggt cggctcgttc gccctgctcc tcctctctat cgccgtcacg
       61 ggtctcctgc tcgtggaccg cgacatccag cggtcgctcg accgcgtgga gaccgacttc
      121 cgcaggctcg gcgctgccgt cgcatcgggc ttccgcggct ggagaacgcg ccgggcggag
      181 cggcgtgagc tcgtcgcgcg ggtgcgcaac gagcgggcgc gcgcggagcg gcaggccgca
      241 ccccgcaccg cggaaatcct ggtcacgcgg atcccggagg ccgatcggac atctcccccc
      301 cctccccgat ccaccgctcc tgggacaccg gacctcaccg tcaacgtcgt cgagcccgtg
      361 cgcgaggcgc gcgcggatca cgtgccgcgt cggcccgtga tcgagtcgga tttcgagtac
      421 gaaatgccgt cgctggacct gctggacgag gacgagggag cccgggcgcg tgtcgacgac
      481 cacgagatcg aggagaacaa gcggatcctg ctcgacaagc tctcgacgta caacatcgag
      541 atcacggcga tcgacgccat cgtcggcccc acggtcaccc tgtacgagct gaccccggcc
      601 ccgggcgtga agatcagccg catcaccgcg ctcgagcacg acctggcgat ggccatggcc
      661 gcccgggccc tgcgcatgat cgcgccgatt cccggaaaga cggcgatcgg cgtcgaaatc
      721 ccgaaccggc accgcgagct cgtgcgcatc cgcgacgtga tcggcaccca gcggttccgg
      781 gacgcccaca tggagctccc gctcatcctc ggaaagtcga tcgagggcga ggtggtcctg
      841 caggacctga ccaggctgcc ccacgtgctg atcgccggcg ccaccggatc gggaaagtcg
      901 gtcggcctca acacgttcat tgccgggatg ctgtacgcct gcccgccctc gcacgtcaag
      961 ttcgtcatga tcgatccgaa gaagatcgag ctggcggcct acgcacgact cgaggcgcac
     1021 tacctggcga tgcctcccga ggccgacgcg gccatcatca cggacttcgg ccaggccctg
     1081 ggcacgctga agagctgcga gatcgagatg gacacgcgct acgacctgct ttcggaggcg
     1141 cgcgttcgct cggtcaagga atacaacgac cggttcgacg cgggccagct cgaccccgac
     1201 gagggacatc gccggctccc ctacatcgtg gtcgtggtgg acgagctcgc cgatctcatg
     1261 atgacggccg gcaaggacat cgaggccccg atcgcccgcc tcgcgcagaa ggcgagggcc
     1321 gtcggcctgc acctgatcct cgcgacgcag cgaccgtcgg tggacgtcat cacgggcctc
     1381 atcaaggcga atttcccgtc acgcatcgcc ctgcaggtcg cctccaagat cgactcgcgg
     1441 accatcctcg accagaacgg cgcggaaggc ctcgtcggca acggcgacat gctctacatg
     1501 cacggcagcc gggttacccg cctccagggg cccttcgtgt cgatcgagga gatcgagcgg
     1561 gtgaccgagt tcatcggcag ccaggagggc ccgggcccat atctcctgcc gtcgctcgag
     1621 gacggcgggg gcatgggcac ggacgcgggc gcgggatcgt cgtccgagcg cgacgaactg
     1681 ttcgaggagg ccgcccgcat catcgtccgc agccagcagg gctcggtgtc gctgctccag
     1741 cgcaagctgt ccgtgggata ctcgcgcgcg gcgcgcatcg tggaccagct ggaggacgcg
     1801 ggcatcgtgg gtcctttcga gggctcgaaa gcccggcagg tgcgcgtggc ggacgagacg
     1861 gaactggaca acctgctcgc cgcagactga aattccccat accagacaga acgacagtat
     1921 atgagcgacg caacccctct cgttggcatc accatgggca gtgaatcgga ctggccgatc
     1981 atgcgggaag cggcggacgt cctgcgccgc ttcggcgtgc cgttcgaggt ccgggtgctc
     2041 agcgcccacc gcacgcccga cgcgacgcag gcgtgggcct cgggagccgc ggcacgcggc
     2101 atcaaggtac tcatcgcagg cgccggcggc gcggcgcacc tcgccggcgt cgtggcggca
     2161 gcgacccacc tgccggtcat cggcatcccc gtcccgtcga agcacctgca gggcctggac
     2221 tcgctgcttt ccatggtcca gatgccccgt ggagtgcccg tggccacggt cgccgtgggc
     2281 ggcgccgcga acgccgggct cctcgccgtc cagatcctct ccacggccga cgcagccctg
     2341 gccgatgcca tgcgggctta caaagacgag atgctgggca aggtggacga gatggaccgc
     2401 cgggtcaggg agtccctcgc ctcggacgcc tgacgcctca ctccaggtac aggtccagct
     2461 cgcgcccggc ctcgcgcccg ctcacgcggt cgcgcacccg gaggcgcagc gagtacagtc
     2521 cctcctcctg gttggacgcg tccaggatca ggtactgccc gtcttcggtc gaggccacgc
     2581 tgatgggcag tcccgccgaa acgccgcccc ggcccccgcc gaacaggccc cggaaaaacc
     2641 gggacacgcc cgagccaccc tccttcggca cgagagcggc ctccacctcg tactcggctc
     2701 gccccccgtc gcccatctcc aggttgtaga cctcgaaata gaggtagatg ggctgctcgt
     2761 gcgagaagac gctccacgga gccggcatga tcgagagccc gcgccgcacg atgtcggccc
     2821 cggtgatcgg acggccgtcc ggtgtctctt ccacgcggta ggccaggagc acgtcgctca
     2881 ccgagaggtc ggttcccgag aagtcgggca gggtaaccga tcggcgctgc acgccaaccg
     2941 tgttgccgga ggtcgtctcg aactccaccg agacgtccac gcggcccggc gcgacgttca
     3001 gctcctccga gtccacccag aggttggcct cccggaactg aacgacctgg tcggtcctca
     3061 ggccgtagag cgtccgccgg cgctcggcca ggatgtcccg gtccgggctg acgacgaaaa
     3121 ggcctgcgct ggccgtgacg ttgatgactt cacccggctg cgcgaactcg ctgatcggaa
     3181 tagcatagtt gacgaggaga tccgtgaccc cgcccggccc ccggaaggac gccaccagga
     3241 acggcagctc gatctcgcgg cccggagcct cgtactcgaa ccggtccgga aggtcacgga
     3301 aggtctcccg cgcccggatc acgtagtcgt tcacccaggg ctgagcgcca tcggcgatcg
     3361 ctgccgccgg cggactgtac atccggtact cgccgttacg gaagggatcc tcgaagacga
     3421 acttgaagtc gccgtacgac cagacgttat acgtgttggc ctcctcgaac atgtcgaagc
     3481 ccgtgccctg cttggcgacg gccaggatcg ggtccgccac ctggctcgga tcggtcgccg
     3541 tgggcagcct ctcctcgctc cggaccgcca cgcccgacga tgatttgggc acgatgacca
     3601 cgtccgtgag cggtggaccg tatcggatca ggatccggcc gcgctcggtc tcccatcccc
     3661 gcaggttcac gtccggggct ccgtagagca ggtccgcgta ggtgatccgg gcgtagtgct
     3721 cgaccttgcg ctcgttgtac ggggtcagga accgtggatc cttgctggtc cagaaacgcg
     3781 acgcataacc cgccgggtcg gcctcgtatg ccgctttctc gtcctccggc acgatgatgc
     3841 ccaggtcgtc gtatgccgtg cgggtgtccg cgtccatgaa ctcgaacgcg ctctcgaacg
     3901 ccttcgccgc cgcatcgagg ttcccggcgc ggtggtgggc caggcccagg taggtccaca
     3961 gttccggatc gtcgggaaag aagacgtaca tctgctggag catcgccaga gcgtccggcc
     4021 attccccctt gagcgcgaag atctccatga ggtagtcgta gaccgagcga tgcctcgggt
     4081 ctgactccag cgcccgcttc aggtgcatga cggcccggtc gtagacccgc tgggcccggc
     4141 cggccaggct ctggatcaga acaccctggg actcgagggc ctcaaggtcg aactcgtccg
     4201 cgagaaacac cgattccgcg tcatatatgc cggcaaacgt cgccgcgaag tcgccggcga
     4261 tctcggtgtc gaggtccgga ttcgtgctga tgtcggcgag ctggtcctgg aggcgcgcgg
     4321 caaccggatc cgacgtggtc ctgccgcgat acgaatagtc ccgatacagc atcgtcggca
     4381 gcatcacggc gttgcggtag cgccagaaat ccctgatgta gctggccccg agctcctcgt
     4441 gcgcgaacgc gttggtcgag tcgagcgcca ggatcctgtg cgccagcgtg aggcgctggc
     4501 tctcgcgcag cttttcgacg atgaagttcc agctctcggc gcgcagctgc tgcatgcgcg
     4561 cgacgaggta ctgaacgttt tcgggctcga tttccagtgc gcggtccagc tcgctgcccg
     4621 cccggccgac gtcacgcagc ggagtctcga agaagatccg agcgagcagg aagtgcgcct
     4681 cggcgttcgc gggatcggcg tccagggccc gttcgaaggc ccgcacggca gcgtcgtagc
     4741 gctcctcccg gaaagcgagg atgccctcgg tcatcggatc ctgtcgctcc tgggctcccg
     4801 ccatgagcgg gaccgcgagc agggtggcga gcatcaaaag gcgtcgcatg gagaatgcgg
     4861 ggcgttcgtg tcgtggtcgg ccgcggacac cgtggggcgc cgggcatcgg gttcatacgc
     4921 cgggtcggaa gccatgattc gccgggtacc cgtccgtcgt gccgtggcgg gcgcgaaacg
     4981 tccggacacc gggtgatccg tccgaaaata gcgtccgggc ccgacgagcc gatccgcacg
     5041 gtgcacggac cgtccgatcc gcggcccgga cggcactgcc gcgtgcccca tccgccggac
     5101 cgcggcccgt tcagtcgtcc cggcccgact tgcggacgcg gtcgagcttt cgcttgctct
     5161 cccggtccgc aatgtcgaca cgcttgtcgt actgcctctt cccgcgggcg agcccgagaa
     5221 gcacctttgc gtatccgcgc cggaagtaga gcttgagcgg aatgatcgtg gcgcccttct
     5281 gtcggacggc ccggtcccac ttctcgatct cccggcgatt caggagcagc ttccgcgggc
     5341 gcagcgggtc gtggttctcg cggttaccga attcgtacgg agagatgtgg cactgcatga
     5401 gcatcagctc ccctccctgc atgacggcat aggcgtcctg caggttggcc ctgccggccc
     5461 gcagcgactt cacctccgtg ccagtcagca cgagcccggc ctcgacggtt tcgtggatgt
     5521 ggtactcgtg ccgggccttc cggttggtga ccaccgtctt gattccctgt tcctcgtcgc
     5581 tcacgggtgc atcctcgctc agatcctgaa ctcggcgtcg tcgtcgtcat ccggctcggg
     5641 cgcgggggcc gcctccggat ccggagcgaa ccggaggtcc ggcacgtcct cggtcacctt
     5701 gtagaagaac aggagctcgg cgagttcggt caggctggcc gtcgccaagt cgtccatccg
     5761 gaaacgcagc gtcgtgtccg acaccagctc cgtgatcggc tcctccacgt cactctcgtc
     5821 ccccgacggg ccgaacgcct ccttcagcgc gagggacacc cggtcgtccg actccccgac
     5881 cctcgaggcg atcagggccc agaccatggg gtaggcggtg agctcgaacc actcctggcc
     5941 caggtccagg gtaatccccg cttcctccgg cgcggtcgga tcgcgtccga cgagcagggg
     6001 cccgacatgc agaacactgc ctgccgcggc gtcgggctcg tcgcccgggg ccgcctcgac
     6061 cacggccggc tgcatcccgt agtgctcgcg gagcacgatc cgcgccatga gcctctcctg
     6121 gcccagcccg gcggcacagg aaagggtacc cggctgacgc tccaggccat cccgaagatg
     6181 gatcgatgca tggggcgtgc accaggccga catggcgacg gtggggtaca catcgtaggc
     6241 atccggggcc gagagcacgc tcaccgtcgg gacgagcgcc gcctccacga gcccggcgcc
     6301 gagccgccgc tcgcattccc tcctcggaag ccgttcgatc gtgacgccga agcggcggca
     6361 ggcgtccgcc agatccgcgg cgaaagcccc gagggcctcg tcgtcccaga gcgccagccg
     6421 acagggttct ggtgtgtcca tggggatggt ccgaaaacgg aaagaccgcc gacgaacggg
     6481 tcgccggcgg tcgatgcatc agcggaatgc ccgagcttcc gccggtctcc tagcgcgtgc
     6541 gctcgcggat ccgggccgac ttgcccttcc gctcgcgaag atagtacagc ttggcacgac
     6601 ggacgcggcc gcgacgcacg acctcgatgc gcgccacctt gggcgagtgc agggggaaga
     6661 cccgctcgac acccacgccg ttcgaaacct tgcggacggt aaaggtccgg cgcgccccgg
     6721 atcccttctc ggcgatcacg acgccctggt actgctggat acgctccttg tcgccctcga
     6781 tcacgcgcac gtgcacgttg accgtgtccc ccggctggaa gtccggggtt tcctcgcgca
     6841 actgcgtacc ctcgacgagc gtcatcaggt ccgttgccat gggacacacc tcctggtttc
     6901 gatgctattc ggttcgactg gattcggcac gccaggcctt catcaggtcc ggccgccgca
     6961 agcgggtttt gtccaggcgc tgcgcctcgc gccacgcctc gacccgggcg tgatcgcccg
     7021 acaacaggac gtccgggacg tgcatgtccc tgaactccgc cggccgcgtg tacacggggg
     7081 cgtcgagcag accgtcctgg aaactgtccg aaagggccga ctcggcgtcc cccagggcac
     7141 ccgggatcag acgaacgacc gcgtcaacaa gaacgagtgc gggtagttcc cccccgctga
     7201 ggacgtagtc cccgatcgag acctcccgcg tcacgagggc gtcccgcacc cgctggtcca
     7261 cgcccttgta gtgccccgcg atgagcacca gccgcgatgc gagcgagagc tcgttcgcga
     7321 ggtcctgcgt gagcgtctcg ccgtccggcg taaggaagat gacctcgtcc gcgcgctcgc
     7381 cgtcggcgag caggccctcg acgcactcga agagcggctc gggcttgagc accatccccg
     7441 caccgccgcc gaacgggtag tcgtccacct gccggtgacg gtcgtgcgcg tgctccctca
     7501 ggtcatggac ccggatatcc acgagaccgg agtcctgcgc acgccgaacg atgctctggc
     7561 gcagcgggct ctcgaagatg cccggaaggg ccgtgaccag gtcgatgcgc atcggctcag
     7621 gccacctcct cctggctgtc cgggtcgagg agtccctcga cgtcccgcac gacgatgcga
     7681 acctcgttta cgctctcgat gagagcgggg acgaacggca cgagcgcgtc gcctccggtg
     7741 tcccgccgca ccacgagcac gggctgcgcg ggaagatcga gcacgtccga gaccacccgg
     7801 ccctccggcc gtcggcgtca tggacctcta ggccgaccag gtcgctcacg aacacctcgt
     7861 cgtcctccag cggggggagc gcatcctcct cggcccagac cgtcagcccg gacacctcct
     7921 cggcggcctc gcgggacgcg agaccctcga gcgacaggac cacggccatg ccggactgca
     7981 tcggctggta gcgcacgccc gtcacgcggt acgcggcggc atcggcc
//
LOCUS       DBNHQZ010000106         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_842817, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000106 DBNHQZ010000000
VERSION     DBNHQZ010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_842817"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="AC16YU_02680"
     CDS             <1..173
                     /locus_tag="AC16YU_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR6064155.1"
                     /translation="SNTSELLEHAKTVERGILIQKNNEPYAVLISYELYDKLLSATPA
                     PRKRGRKKAATT"
     gene            complement(166..1929)
                     /locus_tag="AC16YU_02685"
     CDS             complement(166..1929)
                     /locus_tag="AC16YU_02685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6064156.1"
                     /translation="MTVPSPAPPSLLERYTAHYPEWAKEFARKYFTKTLNPFILYGNV
                     RDLVPTVDAGGRREYLSLRDFLVRDLFSARDIVLFYDRSSGIHFANRESQADFNRALS
                     GYDTIFGTDFARRMPKDPVRVMSVLENYFRLRLGEGKRIACIIDYAETVVPMAEASMY
                     SPDDRNSIVFLQKWAHDPLFLESDFTVILVAENLTELNQQIVQSAYTAEIKLPIPGER
                     ERLDYANWFLQDREDAYRLHSTVSAQGLASNTAGLGYIQLRTILADVIENRTDLTHEV
                     LSELKKDFIEAEARGMLEFVETTWNLDLVAGHTEAKAHLRHAARALRNGRPDVLPMGY
                     LVSGPVGTGKTFMITCFSGEIGIPMVKLKNFRSQWQGQTEANLERILGLLEAMTPVAV
                     MIDEADAALGNRDTQGDSGVSKRVFGQIAAFMSDAKHRGRVIFFLVTARPDFVPVDLK
                     RQGRAEEHIALFYPSTREEREELLRVMLRRTGVDIPIESIPESLLNGDRTFSGADMEA
                     LLTRAKFRAATSPDGKVTAEILEQVVQDFMPPTYPLEVELQTLVAVLECTSQALLPER
                     YRTMDRESIVRRVDELKRLIK"
     gene            2199..>2770
                     /locus_tag="AC16YU_02690"
     CDS             2199..>2770
                     /locus_tag="AC16YU_02690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6064157.1"
                     /translation="MKRSLLPLLFLVVVAVPAVARQAAPPPGFDEYVARVLDAFEVPG
                     VGIAIVKDGEVVLARGYGVRDITTGEPVDGHTLFGIASNTKAFTATAMALLVEEGRVR
                     WEDRVIDHLPWFRMWDPFVTRELTIRDLFVHRSGLGLGAGDLLWWPPSDLDRRTISER
                     LRYLKPATSFRSAYAYDNVLYTVAGEVIEAV"
BASE COUNT          438 a          888 c          950 g          494 t
ORIGIN      
        1 gctcgaacac ctcggaactt ctggagcacg ccaagaccgt ggaacggggc atactcatcc
       61 agaagaacaa cgagccctac gcggtgctca tcagctacga gctgtacgac aagctcctct
      121 ccgccacgcc ggcgccgcgg aagcgcgggc ggaagaaggc tgcgactact tgataagccg
      181 tttcagttca tccactcgac ggacgatcga ttcgcggtcc atggttcggt agcgctcggg
      241 caggagagcc tgtgacgtgc attcgagcac ggcgacgaga gtctggagtt ccacttccag
      301 ggggtacgtc ggcggcatga aatcctggac cacctgctcc aggatctctg ccgtgacctt
      361 gccgtccggg ctcgtcgccg cgcggaactt ggcgcgcgtc agcagggcct ccatgtcggc
      421 gccgctgaac gtccggtcgc cgttgagcag ggactcgggt atcgattcga ttggaatgtc
      481 gacgcccgta cggcgcagca tgacccgcag cagctcttcg cgctcctccc gggtcgacgg
      541 atagaagaga gcgatgtgct cctcggcccg tccctgccgc ttcaggtcga ccgggacgaa
      601 gtccgggcgc gcggtcacca ggaagaagat gacccgtccc cggtgcttcg cgtcgctcat
      661 gaaggccgcg atctgtccga acacccgctt cgagacgccg gaatccccct gggtatcccg
      721 gttccccagc gcggcgtcgg cctcgtcgat catgacggcg accggggtca tggcctcgag
      781 cagcccgagg atgcgctcga ggttggcctc ggtctgcccc tgccactgcg agcggaagtt
      841 cttcagcttc accatcggga tgccgatctc gccggagaag caggtgatca tgaaggtctt
      901 cccggtgccg accgggccgc tcacgaggta gcccatgggg aggacgtccg ggcggccgtt
      961 ccgcagcgcg cgggccgcgt gccggaggtg tgccttggcc tcggtgtgcc ccgcgacgag
     1021 gtccaggttc cacgtagtct ccacgaactc cagcatgccc cgggcctcgg cctcgatgaa
     1081 gtccttcttg agctccgaga ggacctcgtg ggtgaggtcc gtgcggttct cgatcacgtc
     1141 ggcgaggatc gtgcggagct ggatgtaccc gaggccggcg gtgttcgagg cgaggccctg
     1201 cgcggacacc gtgctgtgca ggcggtacgc gtcctcgcga tcctgcagga accagttcgc
     1261 gtaatcgagc cgctcccgct cgcccggaat ggggagcttg atctcggccg tgtaggcgct
     1321 ctggacgatc tgctggttca gctccgtgag gttctcggcg acgagaatga ccgtgaaatc
     1381 gctctccagg aagagcgggt cgtgggccca cttctgcagg aagacgatag aattcctgtc
     1441 gtcgggagag tacatcgacg cctccgccat gggcacgacg gtctcggcat agtcgatgat
     1501 gcatgcgatc cgcttgccct cgccgagccg gaggcggaag tagttctcga gcaccgacat
     1561 cacccggaca gggtccttgg gcatccgccg cgcgaagtcg gtcccgaaga tggtgtcgta
     1621 gccggagagc gcacggttga agtccgcctg cgactcgcgg ttggcgaaat ggatgccgct
     1681 ggaccggtcg tagaagagga cgatgtcgcg cgccgagaat aggtcccgga ccaggaaatc
     1741 gcggagagag aggtactccc gccggccgcc ggcgtccacc gtcggcacga gatcccggac
     1801 gtttccgtac aggatgaacg ggttgagcgt cttggtgaag tacttgcgcg cgaattcctt
     1861 ggcccactcg ggataatggg ccgtgtaccg ctcgaggaga ctcggagggg cgggggacgg
     1921 cacggtcatc tgcaccgccg ccggtccggg cgaaagggcg ggctcctccg cagggggctg
     1981 ctgcgcctcc ggcttctcgg acagggtttc cggttcggag gttttcgctg cgtcttccgg
     2041 catgatcggg gcgtagggct ggttctgaag ccatgcagta tacgcccggc gccggagcgg
     2101 tttcgagtcg gcgcaacttc gggcgaagcc ccgtcccggc cgggagcatt gggttagtgt
     2161 cgctatactt caggcactcc aacctccggg tctccgccgt gaagcgttcc ctgttgcccc
     2221 tccttttcct ggtcgtcgtc gcggtgcccg ccgtcgcccg ccaggccgcg cctccaccgg
     2281 gattcgacga gtacgtggcc cgcgttctcg acgcgttcga ggtgccgggc gtcggcatag
     2341 ccatcgtcaa ggacggcgag gtggtcctcg cgcggggcta cggcgtccgg gacatcacga
     2401 cgggcgagcc ggtggacggg cataccctct tcggcatcgc gtccaatacc aaggccttca
     2461 ccgccaccgc gatggcgctc ctggtcgagg agggtcgcgt ccgctgggag gaccgggtga
     2521 tcgaccacct tccgtggttc cggatgtggg accctttcgt gacgcgcgag ctcacgatcc
     2581 gcgacctgtt cgtgcaccgc tccggcctgg ggctcggtgc cggcgacctg ctctggtggc
     2641 cgccgagcga cctggatcgg cggacgatca gcgagcgact tcgctacctg aagcccgcga
     2701 ccagtttccg cagcgcgtat gcctacgaca acgtgctcta cacggtggcg ggcgaggtga
     2761 tcgaagcggt
//
LOCUS       DBNHQZ010000107         8515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2333477, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000107 DBNHQZ010000000
VERSION     DBNHQZ010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2333477"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="AC16YU_02695"
     CDS             complement(<1..408)
                     /locus_tag="AC16YU_02695"
                     /EC_number="3.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031176 - endo-1,4-beta-xylanase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HIR6064158.1"
                     /translation="MIHHRLLAAMLAASCLVVAAGCSSTDAPPDDDDLVDDSTLRGAA
                     ARHDLAIGVAVGTGLQRTDARFAQVLAREFNILTAENTMKPAAIQPTQGTFAWSEPDQ
                     LVTFAEAHDIQVRGHTLLWHEALPSWMTSSTWNR"
     gene            complement(436..1149)
                     /locus_tag="AC16YU_02700"
     CDS             complement(436..1149)
                     /locus_tag="AC16YU_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6064159.1"
                     /translation="MKPLIRTKLADELAERIAQLIRSGRYCPGDRLPSIAAMSKQFGV
                     GHPTLREALKRLESIGVLDIRHGSGVYVGRHPDMIVVSNPVHSGVASKQVVLDLIQAR
                     MAIEVATVSLAAERASRSSIAAMEALLEEAGRNLGDDALLSASNMAFHREIARASGNT
                     VFAQLLDVLTTLFQNEQRVILDVHGSREADHQEHREILDAIRRHDPVAARERMAAHLG
                     GVRDKMELWEPDRLPVAVP"
     gene            1382..3664
                     /locus_tag="AC16YU_02705"
     CDS             1382..3664
                     /locus_tag="AC16YU_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR6064160.1"
                     /translation="MTPPYRDASLSTDQRLRDLIGRMSLEEKIAQMQCVWNEKAQKLV
                     DESGAFDPVRAGRAFGDGRGLGQVGRPSDAGGGRTARQQAELTNAIQRFFVEESRLGI
                     PVVFHEECLHGQAAPEGTSFPQPIGLAATFDPDLVEALYAMTAHEARSRGTHLALTPV
                     VDVARDPRWGRVEETFGEDPHLVARMGAAAVRGFQGDATFRDGRHVMATLKHFAAHGQ
                     PEGGMNCAPANVSERELRDTFLYPFRVAIQEAGAVAVMPSYNEIDGVPSHANRWLLRK
                     VLREEWGFDGFTVSDYYAIWELHDRPETHGHHVAADKREAAVLAVRAGVNIELPEPDC
                     YLHLADLVREGALAESELDDLVAPMLRWKLEMGLFDEPYVDPDEADRIVGCDAHTALA
                     LRAADSVITLLKNEGPLLPLDPSAAGTVAVIGPNADRSLLGGYSGVPKRDVSVVAGIR
                     ERLGDGARVVHAEGCRITVGGSWQEDEVVRPEPAEDRRLIAEAVEVARAADVVILAVG
                     GNEQTSREAWSLDHMGDRASLDLVGAQNELVDAIVATGRPVVALLFNGRPLAAVHLAE
                     SVPALLECWYLGQETGHAVARALFGDVNPGGKLPISVPRSSGHLPAYYNHKPSARRGY
                     LFDDATPLFPFGFGLSYTTFEVSPPRLADASIGVDGETRVSVDVRNTGSRAGSEVVQL
                     YVRDVTSSVTRPLKELRGFRRIRLEPGERRTVTLPVTRDALAFHGVDLRYAVEPGEFE
                     LMVGTSSREEDLQRVTLRVA"
     gene            3690..5141
                     /locus_tag="AC16YU_02710"
     CDS             3690..5141
                     /locus_tag="AC16YU_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6064161.1"
                     /translation="MNRGLVKLSVGEKVGYGLGDTAANFIFQTILVFQLAFYTDTFGI
                     SAAAAGTLFLVVRFSDAITDPIMGLIADRTKTRWGKFRPYILFSALPFGIMAFLTFTT
                     PDFGPTGKLIWAYVTYTLLMAVYTVNNLPYSALSGVMTGDMAERTSLSSYRFVFAMLA
                     AFLIQVLALPMVNHFGNGDDALGYRITIGIFGAAAVVLFVITFLSTKERILPDPAQKT
                     SVRQDLKDLTKNGPWIAMFFLTLFLFITLALRGGVMLYYFRYYLSTEHLQAFMDGLPR
                     ALDFFVRMSGGEPVSVLFSLFNGFGLTLTIVGILFSKALAVRFGKRNVFIAGLAITAV
                     LTAAFILLPPTAIGATFLMEGIRQLAYGFTIPLLWAMMADVADFSEWRNHRRATGIVF
                     SAVVFGLKAGLGFGGAIGGWVLAVYGYVPNVAQTAEALMGIRLTASIYPAIFFAISTA
                     CLFFYKIDLRKELQIADELAARRELALAARSAT"
     gene            5149..6318
                     /locus_tag="AC16YU_02715"
     CDS             5149..6318
                     /locus_tag="AC16YU_02715"
                     /EC_number="3.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031176 - endo-1,4-beta-xylanase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HIR6064162.1"
                     /translation="MTPFARLLLRARRLAPALVAVALVAGCEPATDPVEEPDSAAPAT
                     LKDAAGLRFLVGAAVSDAVVSGAARPESLLAATHFNTITDENVLKWEVVHPAPGVYDF
                     EPADRYVAFGEANRMFIVGHTLVWHSQTPRWVFEDEAGNPKTREALLSLLEEHISTVV
                     GRYRGRVHAWDVVNEALAEDGSLRDSPWRRIIGDDFIEKAFEFANAADPDAELYYNDY
                     SLANAPKAAGAAALVLGLIDSGHRVDGIGEQGHYKMDWPSPSRLDSAIVTLASVGRPV
                     MITELDIDLLPSRSRDRGADVGLTGEMRAGLNPYAEGLPDSMRQALADRYVELFEVLM
                     ERSDMVDRVTFWGVTDRSSWLNNWPIPGRTSYPLLFDREGRPKEAYHAVVEAMSR"
     gene            complement(6346..8331)
                     /locus_tag="AC16YU_02720"
     CDS             complement(6346..8331)
                     /locus_tag="AC16YU_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064163.1"
                     /translation="MVTASDTGSHAAPAGRRRRTSAGAGRTWALLIVPVAFFVLLEVG
                     LRLAGFGTLPPLVVPLGGQVGWMTPNPDVGRRWFRGTPLVPGPAMDPFRARKPDGGLR
                     LVVQGGSSAAGFPYGHGGSFPRMLEQRLALTLPGREVEVINTGMDAVSSYALIDVADE
                     IAALEPDAVLVYAGHNEYYGALGVGSSISAGRARPLVRLVLALGRFRTTHLVRSAVES
                     VTGLLVRPSPEDVPEATLMEALARGRTVEYGSALYRAGLEQFEENLTDLACGYRDAGI
                     PVFLATVVSNERDLPPLSPGPGDGVDEAAWAAARDAAYAAAALDSARAFGAMERWVAL
                     DSASAGPWYGTARLFDAADLREQAADAYARARDRDRLPFRAPSGMNGVIRAVAEACGA
                     ELVDVHAAFVRVSPGGVPGSSLLLEHVHPNVDGYFVMADAFYRALLESDLPVTWGRAV
                     PATAARGQVLVTALDSAIADVHIRRLLSRWPFRTDPEPDTLRIDTEAGRLAVEVLRGT
                     MDWSEATGRLGAWHEARGEYAAALRAGLALAQEQPFRAAPLRVVAGVLTRMGRLEDAL
                     GFAVRSNRIERTPDGLRLEGQLLTRLGRPEDGVVRLEVARGRAPDDPGVLYDLAVAYV
                     ALDRPDDARRVAEHLLAVRPGDPGALRLLARTAPPVR"
     gene            complement(8336..>8515)
                     /locus_tag="AC16YU_02725"
     CDS             complement(8336..>8515)
                     /locus_tag="AC16YU_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064164.1"
                     /translation="IVASVEERTLVRATLDEKRDELRELLAIVLPEERLTDSLFGVAE
                     VRIDTTLFRQMMELR"
BASE COUNT         1279 a         3046 c         2906 g         1284 t
ORIGIN      
        1 gcggttccag gtcgagctcg tcatccacga cgggagcgcc tcgtgccaga gcagcgtgtg
       61 cccgcggacc tggatgtcgt gggcctcggc gaacgtgacc agctggtccg gctcgctcca
      121 ggcaaaggtg ccctgggtcg gctggatggc cgccggcttc atggtgttct cggccgtgag
      181 gatgttgaac tcgcgcgcga gcacctgggc gaagcgggcg tccgtgcgct gcagtcccgt
      241 ccccacggcg accccgatcg ccagatcgtg ccgggccgcc gccccgcgga gcgtcgagtc
      301 gtctaccagg tcatcgtcgt ccgggggagc atccgtgctg gagcatccgg cagcgacgac
      361 gaggcatgag gccgcgagca tcgcggcgag gagtctgtgg tggatcatcc ttcgtccgtg
      421 gttggcggtc gcggatcagg gaacggcgac aggcaggcgg tccggctccc agagctccat
      481 cttgtcgcgg acccctccga ggtgagccgc catgcgctcc cgggccgcga ccggatcgtg
      541 gcggcgtatg gcgtcgagga tctcgcggtg ctcctggtga tccgcctccc gcgagccgtg
      601 cacgtccagg atcacccgct gctcgttctg gaagagcgtc gtgagcacgt cgagcagctg
      661 cgcgaacacc gtgttgccgg acgccctggc gatctcccgg tgaaaggcca tgttcgaggc
      721 gctgagcagc gcgtcgtcgc ccaggttccg gccggcctcc tccaggaggg cctccatcgc
      781 cgcgatgctg ctcctcgagg cgcgctcggc ggcgagcgag acggtcgcga cctcgatggc
      841 catccgggcc tggatcaggt cgagcaccac ctgcttcgaa gccacgcccg agtggaccgg
      901 gttcgagacg acgatcatgt cgggatgccg tcccacgtac acgccggagc cgtggcggat
      961 gtcgaggact ccgatgctct cgagcctctt gagtgcctcg cggagcgtcg ggtgtcccac
     1021 tccgaactgc ttcgacatgg cggcgatcga cggcagccgg tcgccgggac agtaacggcc
     1081 cgaccggatg agctgcgcga tgcgctcggc gagctcgtcc gcgagcttgg ttcggatcag
     1141 cggtttcaag acttcaccaa cccgcctccc gacccttgtc gggcttgtca taaagtgata
     1201 atatgacttt gaagctagtg gtggtcgaat ggccagtcaa gggtcgccgg agccctctat
     1261 cattgctcac cgattgtttg cagtacatag gccttttcgg cgacaatccg gacgatagaa
     1321 gcgcttccgg tcgccctccg gcagcccgtc ccgtcgcatc ggatccgagc caccccgcca
     1381 catgacacct ccctaccgcg acgcatcgct ttcgacggac cagcgcctcc gcgacctcat
     1441 tgggcgcatg tcgctcgaag agaagatcgc ccagatgcag tgcgtctgga acgagaaggc
     1501 gcagaagctg gtcgacgagt cgggagcgtt cgacccggtg cgcgccgggc gagccttcgg
     1561 cgacggtcgc gggctcgggc aggtgggccg gcccagcgat gcgggcggcg gccgcacggc
     1621 ccggcagcag gccgagctca cgaacgccat ccagaggttc ttcgtcgaag agagccggct
     1681 cggcatcccg gtcgtgttcc acgaggagtg cctgcacggc caggcggccc ccgagggcac
     1741 gagcttcccg cagcccatcg ggcttgccgc gacgttcgat cccgacctcg tcgaggcgct
     1801 ctacgcgatg acggcacacg aggcgcgctc gcggggcacg cacctggcgc tgacccccgt
     1861 cgtggacgtg gcccgggacc cgcgatgggg aagggtcgag gagacgttcg gggaggaccc
     1921 gcacctcgtg gcccgcatgg gggccgccgc cgtgcgcggc ttccagggcg acgccacgtt
     1981 ccgcgacggc cggcacgtca tggccaccct gaagcacttc gcggcccacg gccagcccga
     2041 gggcggcatg aactgcgcgc cggcgaacgt ctccgagcgg gagctgcgcg acacgttcct
     2101 gtatcccttc cgcgtcgcga tccaggaggc cggcgccgtc gcggtgatgc cctcatacaa
     2161 cgagatcgac ggcgtgccct cgcacgccaa ccggtggctc cttcgcaagg tgctgcgtga
     2221 agaatgggga ttcgacggct tcaccgtgtc ggactactac gcgatctggg aactccacga
     2281 ccgtccggag acccacgggc accacgtggc ggcggacaag cgcgaggccg cggtgctggc
     2341 cgtgcgcgcc ggcgtgaaca tcgagcttcc ggagcccgac tgctacctgc acctggccga
     2401 cctggtccgt gagggcgcgc tggccgagtc cgagctggac gatctcgtgg cacccatgct
     2461 ccgctggaag ctcgaaatgg gcctgttcga cgagccctac gtggatcccg acgaggccga
     2521 ccggatcgtc ggatgcgatg cgcacacggc gctcgcgctg cgcgcggccg actccgtgat
     2581 cacgctcctg aagaacgagg gaccgctgct gcccctggac ccgtccgcgg ccggaacggt
     2641 cgcagtgatc ggcccgaacg ccgaccgcag cctgctcggc ggatacagcg gcgtcccgaa
     2701 gcgcgacgtg tccgtcgtcg ccggcatccg cgaacggctc ggcgacggcg cgcgcgtcgt
     2761 ccacgccgag ggctgccgca tcacggtcgg cggctcctgg caggaggacg aggtcgtgcg
     2821 gccggaaccc gccgaggacc gacgcctgat cgccgaggcc gtcgaggtcg cacgcgcggc
     2881 ggacgtcgtg atcctggccg tcggaggcaa cgagcagacc tcgcgagagg cctggtcgct
     2941 ggatcacatg ggcgaccggg cgagcctgga cctcgtcggg gcccagaacg agctcgtgga
     3001 cgcgatcgtc gcgaccggcc ggccggtcgt cgcgctgctc ttcaacggcc gcccgctcgc
     3061 ggccgtacac ctcgcggagt ccgtcccggc gctcctcgag tgctggtacc tcgggcagga
     3121 gaccgggcac gcggtcgccc gggcgctgtt cggggacgtg aatcccggcg gcaagctccc
     3181 catctcggtt ccccggtcga gcggacacct gcccgcctat tacaatcaca agccgtcggc
     3241 ccgcaggggg tacctgttcg acgacgcgac accgctcttc ccgttcggct tcggcctctc
     3301 gtacacgacg ttcgaggtgt cgccccccag gctcgccgac gcctcgatcg gcgtggacgg
     3361 cgagacgcgg gtctcggtcg acgtccggaa cacgggctcc cgggccggct cggaggtcgt
     3421 ccagctgtac gtgcgcgacg tgacgagctc ggtgacccgt cccttgaaag agctcagggg
     3481 attccggcgg atccgtctcg agccgggtga gcgccggacc gtcacgctcc ccgtcacgcg
     3541 cgatgccctc gccttccacg gcgtggacct ccgctatgcg gtggagccgg gagagttcga
     3601 gctgatggtc gggacgtcgt cccgggaaga ggacctgcag cgcgtgacgc tgcgcgtcgc
     3661 atgagatcca acacccacag gaggcgcgca tgaacagggg actggtcaag ctgtccgtcg
     3721 gcgagaaggt cggctacggc ctgggggaca cggccgccaa cttcattttc cagaccatcc
     3781 tggtcttcca gctggcgttc tacacggata cgttcgggat cagcgcagcc gcggccggca
     3841 cgctgttcct ggtggtccgc ttctcggacg cgatcacgga cccgatcatg ggcctcatcg
     3901 cggaccggac gaagacgcgc tggggcaagt tcaggccgta catcctgttc tccgcgctgc
     3961 cgttcggcat catggcgttc ctcacgttca cgacgcccga cttcgggccg accggcaagc
     4021 tgatctgggc gtacgtgacg tacaccctgc tgatggccgt gtacacggtc aacaacctgc
     4081 cgtactccgc gctgagcggc gtgatgaccg gcgacatggc cgagcgcacg agcctctcgt
     4141 cctaccgctt cgtgttcgcc atgctcgcgg ccttcctgat ccaggtgctg gcgctgccga
     4201 tggtgaacca cttcggaaac ggcgacgacg cgctgggcta caggatcacg atcggcatct
     4261 tcggcgcggc ggccgtggtg ctcttcgtca tcacgttcct ctcgacgaag gagcggatcc
     4321 tgccggatcc cgcgcagaag acgtcggtcc gccaggacct gaaggacctc acgaagaacg
     4381 ggccctggat cgccatgttc ttcctgacgc tcttcctgtt catcacgctc gcgctgcgcg
     4441 gtggcgtgat gctgtactac ttccgctact acctgagcac cgagcacctg caggcgttca
     4501 tggacggcct gccacgggcg ctcgacttct tcgtccgcat gtcgggcggc gagcccgtct
     4561 cggtcctctt cagcctgttc aacgggttcg gcctcacgct cacgatcgtg ggcatcctct
     4621 tctcgaaggc gctggccgtc cgcttcggga agcgcaacgt gttcatcgcc gggctggcca
     4681 tcacggccgt gctcacggcc gcgttcatcc tgctgcctcc gaccgcgatc ggcgccacct
     4741 tcctgatgga gggcatccgg cagctcgcgt acggcttcac gatcccgctg ctctgggcca
     4801 tgatggccga cgtcgccgac ttctccgagt ggaggaatca ccgccgcgcg acgggcatcg
     4861 tgttctcggc cgtcgtgttc gggctcaagg ccgggctcgg attcggcggg gccatcggcg
     4921 gctgggtact ggccgtgtac ggctacgtcc cgaacgtcgc gcagacggcc gaggctctca
     4981 tgggcatccg cctgaccgcg agcatctacc ccgccatctt cttcgccatt tccaccgcct
     5041 gcctcttttt ctacaagatc gacctgcgga aggagcttca gatcgccgat gagctggccg
     5101 cccgtcgcga gctggcgctc gccgcgcggt cggcaacctg agaacgtcat gacacccttc
     5161 gcccgtttgc tcctccgagc ccgccgcctc gcgccggcgc tcgtggccgt cgcgctcgtc
     5221 gccgggtgcg agcccgcgac cgatcccgtg gaagaacccg attccgctgc gcccgctacg
     5281 ctcaaggacg ccgccggcct tcgcttcctg gtcggggccg ccgtgagcga cgcggtcgtc
     5341 tccggcgcgg cacggccgga gtcgctgctc gccgcgaccc acttcaacac gatcaccgac
     5401 gagaacgtgc tcaagtggga ggtggtgcac cccgcgcccg gcgtgtacga cttcgagccg
     5461 gcggatcgct acgtggcctt cggcgaggcg aaccggatgt tcatcgtggg acacacgctc
     5521 gtctggcaca gccagacgcc ccggtgggtg ttcgaggacg aagcgggcaa cccgaagacg
     5581 cgggaagcgc tcctctcgct cctcgaggag cacatctcga ccgtcgtggg ccgctaccgc
     5641 gggcgggtcc acgcctggga cgtcgtgaac gaggcgctcg ccgaggacgg ctcgctgcgc
     5701 gactcgccct ggcggcggat catcggcgac gacttcatcg agaaggcctt cgaatttgcg
     5761 aacgcggcgg acccggatgc cgagctctac tacaacgact actccctggc caacgccccg
     5821 aaggcggccg gcgccgcggc cctggtgctg ggcctcatcg attccggcca ccgcgtggac
     5881 ggcatcggcg aacagggcca ctacaagatg gactggccgt ctccctcccg gctggactcg
     5941 gccatcgtca cgctcgcgtc cgtcgggcgg ccggtgatga tcacggagct ggacatcgac
     6001 ctcctgccct cgcgcagccg cgaccgggga gccgacgttg gcctcaccgg ggaaatgcgg
     6061 gccgggctga acccgtacgc ggaaggactc ccggattcga tgcggcaggc cctcgccgac
     6121 cggtacgtcg agctgttcga ggtgctcatg gagcgctccg acatggttga ccgcgtcacg
     6181 ttctggggcg taaccgaccg ctcctcctgg ctgaacaact ggcccattcc cggacggacc
     6241 agctacccgc tcctcttcga ccgggaagga cggcccaaag aggcctacca cgccgtcgtc
     6301 gaggcgatga gccgctgacg gcggagccct ttgcccgcgg gtgactcagc gcacgggcgg
     6361 cgccgtgcgc gcgagcagac gcagcgcgcc cgggtctccc ggccggacgg ccaggaggtg
     6421 ctcggccacg cgccgggcgt cgtccgggcg atcgagggcg acgtacgcga ccgccaggtc
     6481 gtacagcacg ccggggtcgt cgggggcgcg tcccctggca acctccaggc gcacgacccc
     6541 gtcttccggc cggcccaggc gcgtaaggag ctggccctcg aggcggagcc cgtccggcgt
     6601 ccgctcgatc ctgttcgagc ggactgcgaa tcccagcgca tcctccagcc ttcccatgcg
     6661 cgtgaggaca ccggctacga cgcgaagagg cgccgcccgg aacggctgct cctgcgccag
     6721 ggccagcccg gcccggagcg cggcggcgta ctcgccgcgc gcctcgtgcc acgcgcccag
     6781 tcggcccgtg gcctcggacc agtccatcgt cccccgcagc acctcgacgg cgagccggcc
     6841 ggcctccgtg tcgattctga gcgtgtcggg ctcggggtcg gtgcgaaacg gccaccgcga
     6901 cagcaggcgg cggatatgaa cgtccgcgat cgccgagtcc agcgcggtga cgagcacctg
     6961 gccgcgagcc gctgtcgcgg gtacggcgcg tccccacgtg accggaaggt cggactccag
     7021 aagcgcgcgg tagaaggcat ccgccatgac gaagtagccg tccacgttcg ggtggacgtg
     7081 ctccagcagg agcgacgagc ccggcacacc gcccggggac acccggacga acgccgcgtg
     7141 cacgtcgaca agctccgcac cgcacgcctc cgcaaccgcc cgtatcacgc cgttcatccc
     7201 ggacggcgcg cggaagggca ggcggtccct gtcgcgggcg cgggcgtacg cgtcggcggc
     7261 ctgctcccga aggtccgcgg cgtcgaagag ccgggcggtc ccgtaccagg gcccggcgga
     7321 cgcggagtcg agcgcgaccc accgttccat ggccccgaag gcccgcgcgc tgtccagggc
     7381 agcggcggcg tacgccgcgt cccgggcggc cgcccacgcc gcctcgtcca cgccatcgcc
     7441 cggcccgggc gaaagcgggg gcaggtcccg ctcgttgctc accacggtgg cgaggaaaac
     7501 cggaattccc gcatcacgat agccacaggc caggtccgtg agattctcct cgaactgctc
     7561 caggcccgcg cgatacaggg ccgatccgta ctcgaccgtg cggccgcggg cgagcgcctc
     7621 catgagcgta gcctccggaa cgtcctcggg cgagggacgg acgagcaggc ccgtcacgct
     7681 ttccaccgcc gatcgcacga gatgcgtcgt gcggaaccgg ccgagcgcga gcacgagccg
     7741 cacgagcggc cgcgcccgcc ccgcagagat cgacgaaccc acgccgagtg ctccgtagta
     7801 ctcgttgtgt cccgcataca cgagcacggc gtcgggctcc agggccgcga tctcgtcggc
     7861 cacgtcgatg agcgcatacg aactgacggc gtccatcccc gtgttgatca cctcgacctc
     7921 gcgtccggga agcgtcagcg ccaggcgctg ctccagcatg cgcgggaagc tcccgccgtg
     7981 cccgtacgga aagccggcgg cggacgagcc gccctgcacc accaggcgca acccgccgtc
     8041 gggcttccgc gcccggaacg gatccatcgc cggtcccggc acgagcggcg ttccgcggaa
     8101 ccagcgtctt ccgacgtccg gattcggcgt catccagccg acctgtccac cgaggggcac
     8161 gacgagcggc ggcaacgtgc cgaatccagc cagccgaagc ccgacttcga gcagaacgaa
     8221 gaaggcgacc gggacgatca gcagcgccca ggtgcgtccc gcgccggccg aggtgcgccg
     8281 gcgacgcccg gccggagccg cgtgggaccc ggtgtcggac gcggtgacca cggggtcagc
     8341 gaagctccat catctgccgg aaaagcgtcg tgtcgattcg cacctccgcg accccgaaca
     8401 ggctgtcggt gagccgctcc tcgggaagca caatcgccag cagttcgcgc agttcgtccc
     8461 gcttctcgtc gagcgtggcg cggaccaggg tgcgctcctc gacgctcgcc acgat
//
LOCUS       DBNHQZ010000108         3978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_436067, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000108 DBNHQZ010000000
VERSION     DBNHQZ010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_436067"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16YU_02730"
     CDS             complement(<1..279)
                     /locus_tag="AC16YU_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064165.1"
                     /translation="MYSRPRTTFHPAVLALAVGLFLSGCDSGSALTGSGADADRSGSP
                     DVAAKVADAPEFSGVRVARINGNLALSLVSLESGLSAIVGMTREEVVAF"
     gene            725..2251
                     /locus_tag="AC16YU_02735"
     CDS             725..2251
                     /locus_tag="AC16YU_02735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR6064166.1"
                     /translation="MRSAIPPILLVAALLLAGCSDNLAPTSGLDDDLASKGGAGSCAS
                     RSNDSSQKLTECVTLDGVRAHQAALQAIAEANNGHRVSGSPGYDQSVDYVADLLVAAG
                     YDVTVQEFQFQTFIQLSPSVLEQVSPPPAGQVANNIMSYSGSGDVTASVTALAGPGAD
                     ATPGCEPADFAGFPAGTIALVSRGACTFAIKATNAYNAGAVGVIIHNNAAGEINGTLG
                     NTFTLDIPVTSVTQDVGQQLAATPALVMRLKTDTFRGIATAQNVLAETTTGSDEHVIV
                     VGGHLDSVNGGPGIQDNGSGTAAILETAIQMAKAKPVNKVRFAFWGAEESGLVGSSYY
                     VNNLSQDERDRIALYLNFDMIGSPNHVFFIYDGDNSDGVGAPPGPEGSAELEQLFESY
                     YDGRGFPYKGADLGGGSDYLPFIFAGIPSGGLFTGASLIKTPEEAAIWGGTAGQQYDP
                     CYHLACDTFDNVNLQALDVNADAVAHATITLAMDKMKEKKVKVAGAIALPQDPGLPTE
                     "
     gene            2494..2913
                     /locus_tag="AC16YU_02740"
     CDS             2494..2913
                     /locus_tag="AC16YU_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064167.1"
                     /translation="MSRATVRLVAAVDALFVTAGPARSRDCGDLARKPSLRGRAVDHP
                     PLRTVHDLRKASGLDAYPSPAAHDVNIAVSRGQAGALRVEAIDLLGRQAAQLANRRGA
                     AGRSELTWSTDGLPSGLHLIRLFDGTRAVSVPVVLAG"
     gene            2948..>3978
                     /locus_tag="AC16YU_02745"
     CDS             2948..>3978
                     /locus_tag="AC16YU_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064168.1"
                     /translation="MCTSLVRTVTAIVVVLAAAVPARSQNCTDAAGKTGLAAAVADGT
                     IARSLRDFQRLSGHGAPVGARAGDCVSETFGRAVVSPSAGSIQSKLLHGALAQTYDGM
                     VWSDFARFYIEYDASDRPIAFVTEYWDEVGDAWVPTSRESSTYNEAGVETSALSEYWD
                     SGVWVPVERTTHLFDGGGEQVGSETESWSGSGWVPRERVIWTTNASGLPVDIESWHHD
                     GFDWTLYSQEHFEYDLEGRVIEWTSTETTPGGFSERVLTAYGQDGSIVQVTQLLIGEN
                     WVDQYRSEMTSDELGRPVQHLMQASDFMGGWRDLFVDYYTYNDVDNWARMESWSMEGD
                     VWVPDSAETT"
BASE COUNT          664 a         1334 c         1330 g          650 t
ORIGIN      
        1 gaaggcgacg acctcctcgc gcgtcatgcc cacgatggcg gagagcccgc tctccaggct
       61 taccaggctc aacgcgaggt tgccgttgat gcgggccacg cgcacgcccg agaactcggg
      121 cgcatcggcc actttggccg cgacgtccgg gctgccggag cggtccgcgt ccgctccgga
      181 tccggtgagc gccgagccgc tgtcgcagcc ggaaaggaac aggccgaccg cgagggccag
      241 cacggcggga tgaaaggtgg ttcgggggcg agagtacatg acagacctcc atgaggacgg
      301 ttcgtgtgat tgcctgaccc cggcccgcat atcggacccg gatcggcgct cggcccccgc
      361 gcgagcgtgc ggctggtgcg tcgggggtag taccggcgca gccgcatggc aatcctcatg
      421 atagcgaccg tgaaggacac gtgaaatgac cggtgtgcag ggaaatcccg agtcggccgg
      481 ggcgggcggc ttcccatcga gcagatcggc cactgcgtgg ctcctgctgc gttctccacg
      541 ggcccatcca ggaaaaaata tatttgtggc cgtccgattg tagcatgcgc gcgcggttcc
      601 taacgtgcgt ggcgctgctg cggtcgagct acccccagtc ccgacgaccg cccgacgccc
      661 ctgccggatc gccgcaccgt gcgcgatgcc ggcattccac tcagaacacc caggtgacgt
      721 atccatgcgt tcagcgattc cccccatcct cctcgtcgct gccctgctgc ttgcgggctg
      781 cagtgacaac cttgccccca cgtcaggtct cgacgacgat ctcgcctcga agggcggggc
      841 cggctcgtgc gccagccgct ccaacgacag ttcccagaag ctcaccgagt gcgtgacgct
      901 cgatggtgtc cgcgcccacc aggcggcgct gcaggcgatc gcggaggcca acaatggcca
      961 tcgcgtgtcc ggctcccccg ggtacgacca gtcggtggac tacgtggccg acctgctcgt
     1021 ggcggcaggc tacgacgtga cggtccagga attccagttc cagaccttca tccagctgtc
     1081 gccgtcggtg ctcgagcagg tgtcgcctcc gccggccggg caggtcgcga acaacatcat
     1141 gagctactcc ggcagtggcg acgtgaccgc atccgtgacg gccctcgcgg gcccgggggc
     1201 ggatgccaca ccgggatgcg agccggccga cttcgcgggc tttccggcgg gcaccatcgc
     1261 gctcgtcagt cgcggcgcct gcacgttcgc catcaaggcc accaacgcgt acaacgccgg
     1321 agccgtgggt gtcatcatcc acaacaacgc ggccggcgag atcaacggaa cgctcggcaa
     1381 caccttcacc ctcgacatcc cggtgacttc cgtcacgcag gacgtcgggc agcagctcgc
     1441 ggctacgccg gctctcgtga tgcgcctgaa gaccgacacg ttccgtggaa tcgcgaccgc
     1501 gcaaaacgtg ctcgcggaga cgaccaccgg cagtgacgag cacgtcatcg tggtgggcgg
     1561 tcacctcgac tcggtgaacg gcggcccggg catccaggac aacggctcgg gcacggcggc
     1621 catcctcgaa accgccatcc agatggcgaa ggcaaagccg gtgaacaagg tgcgcttcgc
     1681 gttctggggg gcggaggagt ccgggctcgt cggatcatcc tactacgtga acaacctgtc
     1741 gcaggacgag cgtgaccgta tcgcgctgta cctgaacttc gacatgatcg gctcgccgaa
     1801 ccacgtcttc ttcatctacg atggcgacaa ctccgacggc gtgggcgcgc ccccggggcc
     1861 cgaaggttcc gcggagctcg agcagctctt cgaatcgtac tacgacgggc gcggcttccc
     1921 gtacaagggc gcggacctcg gtggcggttc ggactacttg ccgttcatct tcgctggcat
     1981 cccttccggc ggactgttca cgggcgccag cctcatcaag acgccggaag aggccgcgat
     2041 ctggggcgga actgcgggac agcagtacga cccctgctac cacctggcct gcgacacgtt
     2101 cgacaacgtg aacctccagg cgctcgacgt gaacgcggac gccgtggcgc acgcgacgat
     2161 cacgctcgcc atggacaaga tgaaggagaa gaaggtgaag gtggcgggcg ccattgccct
     2221 tccccaggac ccgggtctgc ccacggagtg acgcacgcgg gcgtcgccgg gccgtcacgg
     2281 cccacttgac gtccattctc gtcccgggac gatgcaagag ccccggctcc acacggagtc
     2341 ggggctcttg cgtgtttcca ccgggcactt cccgatggca ttcgctttat ctatattcca
     2401 cgacaggaaa cgcggtcggc cgtgtcatgc cccccggcgg gaccgcgatc gccactcggg
     2461 attccatcgc acctcagagt gagaggggat cgtatgagta gagctacggt tcgcctggtc
     2521 gccgcggtcg atgccctgtt cgtcaccgcg ggcccggccc gatcgcggga ctgcggcgac
     2581 ctggcgcgaa agccctctct ccgcgggcgt gccgtcgacc acccgcccct gcgcaccgtc
     2641 catgacctcc gaaaggcatc cggcctggac gcttacccgt cgcccgccgc gcacgacgtg
     2701 aacatcgccg tttcgcgggg tcaggccggc gcactccgtg tcgaggcgat cgaccttctc
     2761 gggcggcagg ccgcgcagct cgcgaatcgt cggggggcag ccggcaggtc ggagctcacc
     2821 tggtccacgg acgggctgcc gagcggcctg cacctgattc gcctgttcga cggcacgcgc
     2881 gccgtgtcgg tgcccgttgt cctggctggg tgagaatatt cgttcgctca ccaaaggagg
     2941 taaccccatg tgtacatccc tggttcgaac agtcaccgcg atcgtcgtcg tccttgcggc
     3001 ggcggtgccc gcccgctcac agaactgcac cgatgcggcc gggaagaccg ggcttgcggc
     3061 tgccgtcgcc gacggaacga tcgctcggtc cctgcgcgac ttccagcggt tgtccgggca
     3121 cggggcaccg gtcggagccc gggccggcga ctgcgtgtcg gaaaccttcg gccgtgcggt
     3181 ggtgtcgccc tccgccggat cgatccagtc caagcttctc cacggcgcgc tggcccagac
     3241 gtacgacggc atggtctggt ccgacttcgc gcggttctac atcgagtatg atgcaagcga
     3301 tcgtccgatc gcgttcgtga ccgagtactg ggacgaagtc ggtgacgcct gggtccccac
     3361 gtcccgcgag tcgtcgacct acaacgaggc gggagtggag acatcggcgc tttccgagta
     3421 ctgggattcg ggagtgtggg tccccgtaga acggaccacg cacctgttcg acgggggcgg
     3481 agagcaggtg ggctcggaga ctgagtcgtg gagcggatcc gggtgggttc cccgggagcg
     3541 cgtcatctgg acgaccaacg cgtcaggtct cccggtcgac atcgagagct ggcaccacga
     3601 cggcttcgac tggacgctgt attcgcagga acatttcgaa tacgacctgg aggggcgcgt
     3661 gatcgaatgg accagcaccg agacgacgcc cggcggcttt tccgagcgcg tgctgacggc
     3721 ctacggtcag gacggctcca tcgtgcaggt cacgcagctt ctcatcgggg aaaactgggt
     3781 ggaccagtac cggtccgaga tgacctccga cgaactgggc cgcccggtcc agcatctgat
     3841 gcaggcgtcc gatttcatgg gggggtggcg ggacctcttc gtggactact acacgtacaa
     3901 tgacgtggac aactgggcgc gcatggagtc ctggtcgatg gagggtgatg tctgggtgcc
     3961 ggattcggcc gaaacgac
//
LOCUS       DBNHQZ010000109         3275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3689336, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000109 DBNHQZ010000000
VERSION     DBNHQZ010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3689336"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC16YU_02750"
     CDS             <1..349
                     /locus_tag="AC16YU_02750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6064169.1"
                     /translation="FGGDRDRGGALSSILMLILAPIAAMLIQMAISRSREFAADRDGA
                     TISGRPRALASALERLHSGVETHRMDANPATAHMFIVSPFAGGMGGLRNLFSTHPPVE
                     ARIERLLAMEQGR"
     gene            372..605
                     /locus_tag="AC16YU_02755"
     CDS             372..605
                     /locus_tag="AC16YU_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064170.1"
                     /translation="MESDYVVVILVAFVGFVALAALLLVPVWRFLRREEEAAKQWTPG
                     KRGAPVERRTNGEEPNRTDAGAGDQGRGSGAGD"
     gene            complement(602..1180)
                     /locus_tag="AC16YU_02760"
     CDS             complement(602..1180)
                     /locus_tag="AC16YU_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR6064171.1"
                     /translation="MAHDSLVRGLYRFQQRIGLTAPEFRAVLAFTGLLLSGSAVEHWR
                     TRAAPVDPAVYAETDAYFRERAALAGLLPSSEPGAAPGLAASADTVRGRSPADSLRAD
                     STVVEPPDALPAPAPDRGPIDVNTADAASLQRLPGIGPALAGRILEFRERNGPFRFVE
                     DLLMVRGIGDRTLERIAPLVTVDGVPAPEPLP"
     gene            1247..2023
                     /gene="recO"
                     /locus_tag="AC16YU_02765"
     CDS             1247..2023
                     /gene="recO"
                     /locus_tag="AC16YU_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR6064172.1"
                     /translation="MSTQRAAIIRTDAVVLRALDYGETSLIATLFTRERGKVGVMARG
                     VRSPRSRFGSTLEPMAHVQAVIHVKPSRELQTLSEASHIRIRPRIGRSVDRLEPAMRS
                     LELVDALMQSDQALPPVLDLLVSVLDVLDTSADTAADGAANAWPWFALHLASLLGLSP
                     RVDRASLERIPDGGAWLMLETGTVAEERGTGEALRAGRAALRAFGISVRADLAAAMRM
                     RMDAPVRHELDRLVDGYMRYHVEDAWPERAARVFGRLHEP"
     gene            2090..2728
                     /gene="lexA"
                     /locus_tag="AC16YU_02770"
     CDS             2090..2728
                     /gene="lexA"
                     /locus_tag="AC16YU_02770"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6064173.1"
                     /translation="MSRRLTPKQRAFFEYLRRYSRETGGWPTYRELIDRFDYRSPNSV
                     TQNLEALLRKGYLDRDDDGYRFADVPEGSERPAGIPIRGVISAGRLQEAVEEQLGTIT
                     LDMLFPDLDRIFAIRVAGNSMQGAEIFDGDYVLLLDDDIPNGGIGAVLYNGETSLKRV
                     YYDQSGLRLEPANDEYSDIHIRPDVFEEVRVLGRYVGHVNRNGIFKRTDARA"
     gene            2808..>3275
                     /locus_tag="AC16YU_02775"
     CDS             2808..>3275
                     /locus_tag="AC16YU_02775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6064174.1"
                     /translation="MKTPSWRKGLLGKGGYWQNGYWYDLHVERRMPLVMPMLEEMLAA
                     LPPLGDDVSVCDLACGTGNAGYAVMSAYPTVRMTLLDQDAELLSLAQAKLNEIREGST
                     MLQATIRADGEALPGGPYEVVVASLALQDIVGQDVQGPEAEGRYELLFQGIYEA"
BASE COUNT          492 a         1092 c         1214 g          477 t
ORIGIN      
        1 cttcggcggc gaccgggacc ggggcggggc gctctcgtcg atcctcatgc tgatcctggc
       61 gccgatcgcc gccatgctca tccagatggc catctcgagg tcccgcgagt tcgcggccga
      121 ccgggacgga gccacgatct ccggccgtcc gagggcactg gccagcgcgc tcgagcgcct
      181 gcactcgggc gtggagaccc atcggatgga cgcgaatccc gccacggcgc acatgttcat
      241 cgtcagcccg ttcgccgggg gcatgggcgg gctccggaat ctcttctcga cccatccgcc
      301 ggtcgaggcg cgcatcgagc gcctgctggc gatggagcag ggccgctgac cgaagcgaga
      361 cctcgcgccc gatggaatcg gactacgtcg tcgtcatcct cgtcgcgttc gtgggcttcg
      421 tcgccctggc ggcgctgctc ctggtcccgg tctggcggtt tctgcgccgg gaggaggagg
      481 cggcgaagca gtggacgccg gggaagcgcg gtgcgcccgt ggagcggcgg accaacggcg
      541 aggagccgaa tcggaccgac gcgggcgccg gggatcaggg caggggctcg ggggccgggg
      601 attagggcag gggctcgggg gccggcacgc cgtcgaccgt gacgaggggg gcgatccgct
      661 cgagcgtgcg atccccgatg ccgcgcacca tgaggaggtc ctcgacgaag cggaaaggtc
      721 cgttgcgctc ccggaactcc aggatgcggc ccgccagcgc cgggccgatg cccgggagcc
      781 gctggagcga tgccgcgtct gccgtgttca cgtcgatcgg accgcgatcc ggggccgggg
      841 cggggagcgc gtccggcggc tcgacgacag tcgaatcggc gcggagcgag tcggccggcg
      901 accgcccgcg gaccgtgtct gccgatgcgg cgaggcccgg tgcggcgccg ggctccgacg
      961 agggcaggag gccggccagg gcggcccgct cccggaagta ggcgtccgtc tccgcgtaga
     1021 cggcaggatc gacgggggcg gccctcgtcc tccagtgctc gacggcggag ccggagagga
     1081 gcaggccggt gaaggccaga accgcgcgga actcgggggc cgtgagcccg atccgctgct
     1141 ggaagcggta caggccgcgg acgagggaat cgtgcgccat gggagggggt ttatccggtt
     1201 gacacccgaa gcgtcgccgc ataacctgcg tcgaccgaaa cgcccgatgt ccacccagcg
     1261 agctgccatc atccgcacgg acgccgtggt cctgcgcgcc ctggactacg gcgaaacgag
     1321 cctcatcgcc actctcttta cccgggagcg gggcaaggtc ggcgtcatgg cccgtggcgt
     1381 gcgaagccca aggagccgtt tcgggtcgac cctggagcca atggctcacg tgcaggcggt
     1441 catccacgtg aagccgagcc gggagctgca gacgctgtcc gaggcgtccc acatccgcat
     1501 ccgcccgcgg atcggccgct cggtggaccg gctcgagcct gcgatgcggt ccctggaact
     1561 ggtggacgcg ctcatgcagt ccgaccaggc ccttccgccc gtgctggacc tgctcgtgtc
     1621 cgtgctcgat gtcctggaca cctcggcgga cacggcggcg gacggggcgg ccaacgcatg
     1681 gccctggttc gccctgcacc tggcctcgct gctgggcctg tcgccgcgcg tggaccgggc
     1741 gtcgctcgag cggattccgg acggcggggc gtggctgatg ctcgagaccg ggacggtggc
     1801 ggaagaacgc ggcaccggcg aggcgcttcg cgccggccgc gccgcgctgc gggcattcgg
     1861 gatctcggtc cgggccgacc tcgccgccgc gatgcggatg cggatggatg cccccgtccg
     1921 gcacgagctc gaccgcctcg tggacggata catgcgatac cacgtcgagg acgcctggcc
     1981 ggagcgtgcg gcccgcgtgt tcgggaggct gcacgagccc tgagcgggca tttgcggact
     2041 ggagtccgac tcgatatatt catgtgttac acatgtgacc gaggcctcca tgtcccgacg
     2101 gctcaccccc aagcagcgtg ccttcttcga gtatctgcgc cgatacagcc gggagacggg
     2161 cggatggccc acgtatcgcg agctgatcga ccggttcgac taccggtcgc cgaacagcgt
     2221 gacccagaac ctggaggccc tcctccggaa gggctacctg gaccgcgacg acgacggcta
     2281 ccgcttcgcc gacgtgcccg agggttcgga gcggccggcg ggcattccca tccgcggcgt
     2341 catctcggcc ggccgcctgc aggaggccgt cgaggaacag ctcggcacca tcacgctcga
     2401 catgctgttt ccggacctcg accggatttt tgcgatccgc gtcgccggca actccatgca
     2461 gggcgcggag atcttcgacg gggactacgt gctgctgctc gacgacgaca tcccgaacgg
     2521 cggcatcggc gcggtcctgt acaacggaga gacctcgctc aagcgggtat actacgacca
     2581 gtcgggcctg cgtctggagc cggccaacga cgagtactcc gacatccaca tccggccgga
     2641 cgttttcgag gaggtccggg tgctgggccg gtacgtcggc cacgtgaacc gcaacggcat
     2701 tttcaagcgg acggacgcgc gggcgtaggc ggtcgggccg cgcggcgggg atctttggcc
     2761 gcaggcgacg ggtgcaaggg tgcttccgat cccaaaactg tgcgcggatg aagactccga
     2821 gttggcgaaa gggactcctg ggcaagggag gttactggca gaacggctac tggtacgatc
     2881 tgcacgtgga gcggcggatg ccgctcgtga tgcccatgct cgaggagatg ctcgcggcgc
     2941 tgccgcccct gggcgacgac gtctcggtgt gcgacctggc ctgcggcacc ggcaatgcgg
     3001 gatacgcggt catgtccgca tacccgaccg tgcggatgac gctcctggac caggacgcgg
     3061 agctgctgtc gctcgcccag gccaagctga acgagatccg cgaaggctcg accatgctcc
     3121 aggcgacgat ccgggccgac ggcgaggccc tgcccggggg gccgtacgag gtcgtcgtgg
     3181 cttcgctcgc gcttcaggac atcgtgggtc aggacgtcca gggcccggag gcggagggcc
     3241 ggtacgagct gcttttccag ggcatttacg aggcc
//
LOCUS       DBNHQZ010000110         4592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1251155, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000110 DBNHQZ010000000
VERSION     DBNHQZ010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1251155"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16YU_02780"
     CDS             complement(<1..398)
                     /locus_tag="AC16YU_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR6064175.1"
                     /translation="MKRTAAWAGAIALALSSCTTVNLGDLATRSTRPQPSPAASATPT
                     PAAAKPGAPFKAPGEVLKDTRAVDGLFKLHMKRDNTVFATLAPGQLGTDFGLVMHFSS
                     GNGTFDIQEGLYLSDTRMMRFVRAADKIHLV"
     gene            590..1000
                     /locus_tag="AC16YU_02785"
     CDS             590..1000
                     /locus_tag="AC16YU_02785"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR6064176.1"
                     /translation="MAQKKSRLIWFWLVAVLFTAWFFTTEEPRASVESWLAERGIAFT
                     RFGSAQAFEVFDTVLVPPAHGTSMIRGTIRNTSDRHYRVVRVLFDLLDEDGRKVGSAV
                     KFLSRVGPGETRSFEAPTHGAGFADFRLKEATGW"
     gene            complement(1149..1730)
                     /locus_tag="AC16YU_02790"
     CDS             complement(1149..1730)
                     /locus_tag="AC16YU_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064177.1"
                     /translation="MKYLSLVWVVASLLILTVGCSADSAVSGALEPGDEDVAAKGAVI
                     HRVAAGSNDACEAFGGYQPGCDGNFSLVANEYADGTVKGMWRDVWMGTETVPPGLLVS
                     IDCLKVEEYEGMPIAAISGTIVNGPDAGLIARAIVRDLGTSNGDTNDEISFALWGGTR
                     TCEDLGDGTGRPWDWFDTRGYFLELEQGQVTIW"
     gene            complement(2006..2965)
                     /locus_tag="AC16YU_02795"
     CDS             complement(2006..2965)
                     /locus_tag="AC16YU_02795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR6064178.1"
                     /translation="MKNLVLAGLEAPPGHSVRGRVPVTAAGITVPLPVALVNGSADGP
                     TLVVTAGIHGGEYPCVEAAARLARSLDPAAIRGRLVIVPSANPVAFRARAIYVTPPDG
                     KNLNREFPGSAGGTFTSAWAHWLFENVIRGADAYIDMHGGDMIEALVPFTAWNRTGRP
                     EVDDVARGMAEAYGIVHVVERDVQGLHGMTHAAAAHAGIPALLTEAGGQGVWDEESVR
                     VLHDGVLRVMAALDMADPLPGRVEPVVVHPGWTWLRSEHDGLFYPEVRVGDVVEPGQD
                     LGRVADVWGETLQALVAPVGGLVLFEVTSLAMNTGDPLLAIAH"
     gene            complement(3143..3964)
                     /locus_tag="AC16YU_02800"
     CDS             complement(3143..3964)
                     /locus_tag="AC16YU_02800"
                     /EC_number="4.99.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIR6064179.1"
                     /translation="MRSILTLFLAGLALPAFAQMRPTGILVMAHGGTDDWNASVHEAV
                     NPIRAHVPVAVAFGMANPMTMRVAVDSLVEAGVEEIRVVRLFLSGESFLESTGYILGL
                     HDRRPTGGMHAEHMHQFDIPVPVSVSGDGLLDAPEVGAILRDRARAMSRDTAGESVLV
                     IAHGAASEEENDRWLRRMDALADSVRAEEFAEVRVETLREDWDEPRAAAERRIRRFVM
                     EQDSLGREVLVIPFRLAGFGPYAEVLDGLDYRSDGTGLLPHPVITDWIARQAASR"
     gene            complement(4171..>4592)
                     /locus_tag="AC16YU_02805"
     CDS             complement(4171..>4592)
                     /locus_tag="AC16YU_02805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6064180.1"
                     /translation="DPEVVESSLRVADVVKLNEDELAQLATWRGWPTGERDSVHALAD
                     AYGCSTVCVTRGERGAAVLREGDWVEHAGFKVPVVDTVGSGDAFLAALLDGILFGRPI
                     GEVLDRACRLGAFVATRTGATPEYDVAGEEAFRWIGA"
BASE COUNT          750 a         1639 c         1502 g          701 t
ORIGIN      
        1 accaggtgga tcttgtcggc cgcgcgcacg aaacgcatca tacgcgtgtc ggacagatag
       61 aggccttcct ggatgtcgaa cgttccgttg ccgctcgaga agtgcatgac gagcccgaaa
      121 tccgtaccga gctggccggg cgcgagggtc gcgaagaccg tgttgtcgcg cttcatgtgc
      181 agcttgaaga gcccgtccac cgcgcgcgta tccttcagca cctcgccggg ggccttgaac
      241 ggtgctccgg gcttggcggc agcaggcgtg ggcgtggctg acgccgccgg cgacggctgt
      301 ggcctcgtcg atcgcgtcgc gaggtcaccc aggttcaccg tggtgcagga ggacagagcg
      361 agcgcgatgg cgccagccca ggctgcggtt cgtttcatgg ttcggaaggc ggatggattt
      421 cggggccgga agatgcgaca gaagacggga aaacgcacac ccgggttgcg ctcgtcgtct
      481 tcgcgcacgc tgcccgggga tcgccgacgg tccgtcgaat gagtgacgac ccgccgaatc
      541 ccgctgcctc gatgccgcgg cacccggatc agggtgtacc ttgcacgtca tggcacagaa
      601 gaaaagcagg ctgatctggt tctggctcgt cgccgtactc ttcacggcgt ggttcttcac
      661 gaccgaagag ccgcgcgcgt ccgtggaatc gtggctcgcc gagcgcggga tcgccttcac
      721 gcggttcggc agcgcgcagg ccttcgaggt cttcgacacg gtgctggtcc cgcccgcaca
      781 cggcacgtcg atgatccgcg gaacgatccg caacacgtcg gatcggcact accgcgtggt
      841 ccgcgtgctc ttcgatctcc tggacgagga cggcaggaag gtgggatccg cggtcaagtt
      901 cctctcgcgc gtcggcccgg gagagacgcg ctcgttcgag gcgccgacgc acggcgcggg
      961 gttcgcggat ttccggttga aggaagcgac ggggtggtga cggtgcgttc gcgcgtggac
     1021 ggacagcgcc ccgaccctgc cgcgcaggac cggggcgccc tgattcctcg gaccggatcg
     1081 ggacaccggg cccaagttca cggggacctc gatggccggc cgagcccact ccccgcgtac
     1141 cgaaccggct accagatggt cacctgaccc tgctcgagct caaggaagta accccgggtg
     1201 tcgaaccagt cccaggggcg gcccgtgccg tcacccaggt cctcgcacgt gcgcgtgccg
     1261 ccccacagtg cgaaggatat ctcgtcgttg gtgtcgccgt tcgacgttcc cagatccctc
     1321 acgatagccc gtgcaatgag gcccgcgtct ggtccgttga cgatcgtgcc gctgatcgcg
     1381 gctatgggca tcccctcgta ttcttcgacc ttgagacaat cgatgctgac gagcagcccc
     1441 ggcggcacgg tttccgtgcc catccatacg tcccgccaca tccccttcac ggtcccgtcg
     1501 gcatactcgt tcgccaccag cgagaagttg ccgtcgcatc cgggctggta ccccccgaat
     1561 gcctcgcagg catcattgct gcccgctgcg acgcgatgga tcactgcgcc cttggccgcc
     1621 acgtcctcgt cgccgggttc cagggcgccg gacacggcgg aatcggcact gcagccaacg
     1681 gtcaggatca acagcgacgc gacgacccac accagggaaa ggtatttcat gacctgtacc
     1741 ccacggttgg tgaaacatgg aaaatccact gactcccccg caccacaagg ggcacacgag
     1801 agtgcgtcac ctcgcctccc tcaaacgagt ttaggggccg tgcgcgatca ttctctgcgg
     1861 gacggatttg gcaatccgtg tgcgcgcggg cctcacgaca ccgcaggaca acgtcctacg
     1921 aagatgacca gggcgtcctg acggtctgcg cccgcagcgc tggagaccag gcgagggacc
     1981 gatccgaacc cgcagggacg atccctcagt gcgcgatcgc gagcagcgga tcccccgtgt
     2041 tcatggccag ggaagtgacc tcgaacagca cgaggcctcc taccggggcg accagcgcct
     2101 ggagcgtttc gccccagaca tcggccacgc ggcccaggtc ctggccgggc tccacgacgt
     2161 cgcccacccg gacctcgggg tagaacagcc cgtcgtgctc cgagcgcagc cacgtccacc
     2221 cgggatgcac cacgacgggt tctaccctcc cgggcagcgg atccgccatg tcgagcgccg
     2281 ccatcacgcg gagcacgccg tcgtgaagga ctcggaccga ctcctcgtcc cagacgccct
     2341 gccctcccgc ctcggtgagg agcgccggaa tcccggcgtg cgccgccgcg gcgtgggtca
     2401 ttccgtgcag gccctgtacg tcccgctcga ccacgtgtac gatgccgtag gcctcggcca
     2461 tgccgcgcgc tacgtcgtcc acctcgggcc gccccgtgcg gttccacgct gtgaacggta
     2521 ccagcgcctc gatcatgtcg ccgccgtgca tgtcgatgta cgcgtccgcc cctcggatca
     2581 cgttctcgaa gagccagtgg gcccatgccg acgtgaacgt ccctcccgcc gagcccggaa
     2641 actcccggtt caggttcttg ccgtcgggcg gcgtcacgta gatcgccctc gcccggaatg
     2701 ccaccgggtt cgcgctcggg acgatgacca accggccgcg gatggccgcc ggatcgagcg
     2761 agcgtgcgag ccgggccgcc gcctccacgc acgggtactc gcccccgtgg atgccggccg
     2821 tgacgacgag tgtcggcccg tcggccgacc cgttcacgag cgcgacggga agcggaacgg
     2881 tgattccggc ggcggtaacg ggaacgcggc cgcgtaccga gtggccgggt ggggcctcga
     2941 ggcccgcgag gacgaggttc ttcatggttt gcctgttcga ccggcggggc acgcccgcga
     3001 cggcttcatg gtaccatccc gtggcggttt tgcgaagagc gtccctcgca agttcgcggg
     3061 atcgtcacgg acgatccggt ccgggaagcg gccctggcca cgcggcacgc tgcaggctac
     3121 agcgacttcc gaccggcagg cctcagcgcg aggcggcctg cctcgcgatc cagtccgtga
     3181 tgaccgggtg cgggagcaga ccggttccgt ccgaccggta atccaggccg tcgagcactt
     3241 ccgcgtacgg accgaagccg gccagtcgga acgggatgac gagcacctcc cggccgagcg
     3301 agtcctgctc catgacgaac cgccgtatcc gccgctccgc cgctgcgcgc ggctcgtccc
     3361 agtcttcccg cagcgtttcc accctcacct cggcaaactc ctccgctcga acggaatcgg
     3421 cgagcgcgtc catccgccgc agccaacgat cgttctcctc ctcggaggcg gcgccgtggg
     3481 caatgaccag aaccgactcg ccggccgtat cccggctcat cgcccgggcc cgatccctga
     3541 gaatggcgcc cacctccggc gcgtcgagca gcccgtcccc ggacaccgac accggcacgg
     3601 ggatatcgaa ctggtgcatg tgctcggcat gcatgccgcc ggtcggcctt cggtcgtgca
     3661 gccccaggat gtagcccgtg ctctcgagga acgattcccc cgacaggaag aggcgcacga
     3721 cgcgaatctc ctccacgccg gcctcgacga gactgtcaac cgccacccgc atcgtcatgg
     3781 ggttcgccat gccgaaggcg accgcgaccg gcacgtgagc ccggatcgga ttcacggcct
     3841 cgtgcaccga ggcattccag tcgtccgtcc ctccgtgggc catcaccagg atgcccgtgg
     3901 gccgcatctg ggcgaacgcc gggagcgcca gcccggccag gaaaagggtc aggatcgatc
     3961 gcacgacggg aacggcttgt ttagactggt tctaaagtac gaatccgtcg tcgtgccgtc
     4021 cgcaaagaac gcatggatcg cagtcagcgg tcgacgagca ccttccagaa gttcgacggg
     4081 tccgcgccgg tcggacaagg gccggagccg tcagggaagt tccggccaaa tcgacgcgga
     4141 gcggagtccg gagacggcgg gctcgcccgg tcaggccccg atccagcgaa aggcctcctc
     4201 ccccgctaca tcgtactccg gtgtcgctcc cgtccgcgtg gccacgaacg cgcccagccg
     4261 acaggcccgg tccagcacct cgccgatcgg acgcccgaac aggatcccgt cgaggagcgc
     4321 cgccaggaat gcgtcccccg acccgaccgt gtccacgacg ggaaccttga acccggcgtg
     4381 ctcgacccag tcgccctctc gcaggaccgc ggcgccccgc tcgccgcgcg tgacgcagac
     4441 cgtggagcag ccgtacgcgt cggccagcgc atggacggag tcgcgctcgc cggtaggcca
     4501 cccccgccag gtggcgagct gcgcgagctc gtcctcgttc agcttgacta cgtcggcgac
     4561 gcggagcgag gattcgacga cctccgggtc ca
//
LOCUS       DBNHQZ010000111         7723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3146455, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000111 DBNHQZ010000000
VERSION     DBNHQZ010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3146455"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16YU_02810"
     CDS             complement(<1..590)
                     /locus_tag="AC16YU_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6064181.1"
                     /translation="MLQVRNILVAKDLSESAGLAFRAAYELAVRMRATLHLLFVQVEH
                     PDPFAPATYPADHRERVHAAMRKVVEEVETERSEPAGDRPELVYVVEAGLAVAPAVVS
                     YAARNEVDVIVSGTHGRRGMRHLMLGSVAEEIVRTAECPVLTVRGTPGDRALFPGPDS
                     DILVPVDFSDHSIRAIRYARELASLFGCGIRMVHVV"
     gene            complement(874..1353)
                     /locus_tag="AC16YU_02815"
     CDS             complement(874..1353)
                     /locus_tag="AC16YU_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6064182.1"
                     /translation="MVEPSTLRAVRVDSRWPSAQPAAGAVQTMVTEIRPGLECRPAAS
                     GRSHWARNVAWRPCSQLRDNGPCYELRMSVHGVQADRLVVEVRDDVLVVTGRLDSGPY
                     SRSSDESDFHRLIGLPADAARTGATARLEGELLVVRIPKARDESGARLHLTVPDDLV"
     gene            complement(1416..2066)
                     /locus_tag="AC16YU_02820"
     CDS             complement(1416..2066)
                     /locus_tag="AC16YU_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6064183.1"
                     /translation="MIRVLIVDDHPLMRKGLVLSMTGEPDVKVVAQASNAEEALDALH
                     AEDPDMAIVDISLPGMSGLELIKHMLALRPLLKILVVSRHDESLYAERAIRAGARGYV
                     MKLEAADVMIRAIRRVMNGGIYVSDEINERLLLGMATGRDGLVKSPLEVLSDRELEVF
                     ELTGRGYGTREIAEKLHLSVKTVESYRARIKNKLNLNSAAELMQHAVQWVEGEGAG"
     gene            complement(2063..3457)
                     /locus_tag="AC16YU_02825"
     CDS             complement(2063..3457)
                     /locus_tag="AC16YU_02825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064184.1"
                     /translation="MAEGSMKVFVVGADEKEMPEALRCPGVRFVPEPGRLRGRADFVL
                     VAVRGPGDVARVRRTRMRVGQGAFIIAVEYTPGTVAAGAYEEAGADDLCLAGETLDRR
                     LPLVLRRIGALIAREKRLETILDTTVDGIIVMDAQGRVQAFNSAAEKIFGYRADEIVD
                     RSVGLLMPEPYREEHDTYVANYLQTGQAKIIGMGREVVGQRRDGSVFPMELSVSEVRT
                     EDSRTFTGVVRDISERRRLENEVLRISDQERRRIGQDLHDGLGQMLTGIGLIAQNLGR
                     RLREQDSELREEVDEITELIREADHQARSLARGLVPVELDANGLAAALQRLCTNAERL
                     FGVECQFEEVGSMPVRDSTAAINLYRIAQEAVSNAVKHGTAPAIRVTLASGREHVRLR
                     IQDNGIGFPERLNEDRGMGVRIMHYRARVIGGLLEIRRRPEGGTLVTCTLPRIANPAL
                     RASAPSHPTNPVAS"
     gene            complement(3575..4096)
                     /locus_tag="AC16YU_02830"
     CDS             complement(3575..4096)
                     /locus_tag="AC16YU_02830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR6064185.1"
                     /translation="MKSLLLLRHAKSDWDSGPGDDHDRPLSGRGRKAAARMGLFIAEA
                     GLVPDLCLTSTAVRAADTLTRAARHGGWDHVPRQDLRSLYGAGPADVSGILAALPDDI
                     DRILLVGHEPGWSEAARVLTGGSSVRMATGTLARIDFEVDRWVDCRVGGGRLSALVTP
                     RLLEALPPPRGSG"
     gene            complement(4101..4472)
                     /gene="folB"
                     /locus_tag="AC16YU_02835"
     CDS             complement(4101..4472)
                     /gene="folB"
                     /locus_tag="AC16YU_02835"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6064186.1"
                     /translation="MPIGTVRLCNAVFYAHHGVMQEEHRIGGRYEVDVAMDLDFEGAA
                     TADDLSRTVDYERVYRVVHDTITHNRFYLIERLAYLIARQVLQESGNLVSVEVRVRKA
                     NPPVGGPCDFAEAVYRSTRGD"
     gene            complement(4526..5461)
                     /locus_tag="AC16YU_02840"
     CDS             complement(4526..5461)
                     /locus_tag="AC16YU_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064187.1"
                     /translation="MRGFLLAACVMAGCTAAPAPSGVAVDAGRSDAGRVLRFYLGSYM
                     DPARIDSVLVADGDGWRLDLESLASTAPELRAQLEPSAGDGLLDRDELRAAVQATYAE
                     ARRLPGSATDLVALPGFGAPDDERVEIRVRGSMTRYERRVRIPVAAVVSALETFAAEG
                     ELRYPAGTVVVGEHLEDGDVVETTAMIRRADGYWDFAAYGVGGEPIGEIHGDRGGLAV
                     PTRCFGCHYGDRAFEPEASFPAPAADGPHGPRFVDVPGEWVDPDVVARLDEHRRRSDG
                     LLGLYATVLVGRWRAEAEAGRLPAERARLVEALGF"
     gene            complement(5458..6444)
                     /locus_tag="AC16YU_02845"
     CDS             complement(5458..6444)
                     /locus_tag="AC16YU_02845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6064188.1"
                     /translation="MRIAVTGSIAYDYLMRFPGRFKDHFIADRMESVSLSFLVDSMVR
                     QRGGVAANIAYTMRLLGGEPVIFATVGQDFGDYRTWLEEQGLSTDSIIEIDDDFTASF
                     FVSTDLDQNQIANFYTGAMAHARRFSLADQGLDSVDLVIISPNDPEAMLNLADECRRA
                     AVPFAYDPSQQTARLDGDDLRRSIPGARSLMVNEYELAIIMKKTGWTMDEILGRVDCL
                     VVTSGARGSTIHADGAIHEIPAAVPEQIVEPTGAGDAYRGGFFAALSADLPLSVAGRV
                     GALCSTYVLEQLGTSNHRFGFDEFAGRYERSFGPEPGLSAIRRRVEARMARS"
     gene            6687..>7723
                     /locus_tag="AC16YU_02850"
     CDS             6687..>7723
                     /locus_tag="AC16YU_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064189.1"
                     /translation="MPDFRLTPVRILFSLIALTAPLSRAGAQPVPPNETTSYAEALRT
                     FEDRRFGTAAERFEAFRDRFPRSVHVPDATYHEGAALLALGRDQDAVALLTRFELDFP
                     SHPLAFEARLALGQYYYDNGNAHEALTTLEQVLLGSPPDETAARALYWMGDAAVELDR
                     MEDGLEYYRRAADEYPETETAPKALYARAFTFVSLGRYEEGAQTLERLEARYTQSDLA
                     RNVGLALAEVYYEIGDYERAVAEIRRRLPSLVREVKDRAEFLLAESYNQLRDSENAIS
                     AYRRFTEGPEESPYYRRAVYGLAWNYYHEGAHQWAHEQFARLVAGSDDLAIEALYYEG
                     VNLAMAARGQDA"
BASE COUNT         1234 a         2775 c         2529 g         1185 t
ORIGIN      
        1 tccaccacgt ggaccatccg gatgccgcac ccgaacagcg acgcgagctc ccgcgcgtaa
       61 cggatcgccc ggatcgagtg gtccgagaag tcgaccggaa ccaggatgtc cgagtccggg
      121 ccgggaaaga gggcccggtc gccgggcgtt ccgcgcaccg tcagcacggg acattccgcc
      181 gtgcgcacga tctcctccgc cacggagccc agcatcaggt ggcgcatgcc gcgacgaccg
      241 tgggtgccgg agacgatcac gtcgacttcg ttccgggcgg cgtatgaaac caccgcgggg
      301 gccacggcca ggccggcctc gaccacgtaa accagctcgg gccgatcgcc ggcgggctcg
      361 ctcctttcgg tctcgacctc ttccaccacc ttccgcatgg ccgcgtgcac gcgctcgcga
      421 tgatcggcgg ggtacgtggc cggcgcgaac gggtccggat gctccacctg gacgaaaagc
      481 aggtggagag tcgcccgcat ccgcacggcc agctcgtacg cggcccggaa ggccaacccg
      541 gccgattcgg acaggtcctt ggccacgagg atgtttctga cttgcagcat gtcagtctcg
      601 cctcccccgt ccgggcggcg gttgggcttc acccctgcac cgttcgggcg ggcgtgttgt
      661 ttgccgggtc tctatgcagg ctgtacgcat cattgctcaa gcaacgtacg tcgttctgca
      721 tccggcgtat gccgggaagc tctcgtcgct catgtggggg ttaacgttcg cgtcgaatgc
      781 gggcacccgc gccccgcaat gcagggagtt tccctacggc aggtgcaggg gtgcggatga
      841 cggtagccgt acgatgcgcc gggagccgcc ccgtcagacc aagtcgtccg gcaccgtgag
      901 gtgcaaccgc gcgccggact cgtcccgcgc cttgggtatg cggacgacga gcagctcacc
      961 ctccagtcgc gctgtcgcgc cggtccgggc cgcgtccgcc ggcagaccga taagccgatg
     1021 gaaatcggac tcgtcgctgc ttcgcgaata cggtcctgaa tccagcctcc ccgtgacgac
     1081 caggacatcg tcccggacct cgacgacgag ccggtcggcc tggacgccgt gcacggacat
     1141 cctcagttcg tagcagggac cgttgtcccg aagctgcgag caggggcgcc acgccacgtt
     1201 gcgcgcccag tgcgatctgc cggatgcggc gggtctgcac tccagacccg gcctgatctc
     1261 ggtaaccatg gtctgaactg ctccggccgc cggttgggcc gacggccacc gactatctac
     1321 acggacggcc cgcagggtgg aaggttccac catgcccagc gatccgcacg tccgatcgac
     1381 ccggcagccg ggaattcacg cagcgcccgg aacggtcacc cggcgccctc gccctccacc
     1441 cactgcacgg catgctgcat gagctcggcg gcgctgttca ggttcagctt gttcttgatc
     1501 cgcgcccggt acgactcgac ggtcttcacg gagaggtgca gcttctccgc gatctcgcgt
     1561 gtgccgtatc cacgacccgt gagttcgaat acctcgagtt cccggtcgga cagcacctcg
     1621 agcggcgact tgaccagccc gtcccggccc gtggccatgc ccaggagaag ccgctcgttg
     1681 atctcgtccg agacgtagat accaccgttc atgacccgcc ggatcgcccg gatcatgacg
     1741 tccgcggcct ccagcttcat gacgtatccg cgcgcgccgg cccggatcgc ccgctcggca
     1801 tagagcgact cgtcgtggcg cgaaacgacg aggatcttga gcagcggccg cagggccagc
     1861 atgtgcttga tcagctcgag gccgctcatg cccggcagcg agatgtccac gatcgccatg
     1921 tcgggatcct ccgcgtgcag cgcatcgagg gcctcctccg cgttcgaggc ctgggcaacc
     1981 accttcacgt ccggctcccc cgtcatcgag agcacaagtc ccttccgcat aagcggatga
     2041 tcatcgacaa tgagtacccg gatcatgagg caaccggatt ggtgggatgc gatggggccg
     2101 atgcccgaag ggccgggttg gcgatacgcg gaagcgtaca ggtaaccagc gtgcctccct
     2161 cgggccggcg tctgatttcg agcaggccac cgatgacgcg cgcccggtag tgcataatac
     2221 gcacgcccat gccgcgatcc tcgttcagcc gttcgggaaa cccgattccg ttgtcctgaa
     2281 tcctcaggcg gacgtgctcc cggccggagg cgagggtgac gcggatcgcc ggcgcggtgc
     2341 cgtgcttgac ggcattcgac accgcctcct gggcgatgcg gtacaggttg atcgccgccg
     2401 tgctgtcgcg gacgggcatc gaccccactt cctcgaactg gcactccacg ccgaacaggc
     2461 gctcggcatt cgtgcagagg cgctgcaggg ccgccgccag cccgttcgcg tccagctcca
     2521 ccgggaccag gccgcgcgcg aggctccggg cctggtgatc ggcctcccgg atcagctcgg
     2581 tgatctcgtc cacctcctcg cggagctcgg aatcctgctc gcgcaggcgc cgcccgaggt
     2641 tctgcgcgat gagtccgatg ccggtaagca tctggccgag cccgtcatgc aggtcctggc
     2701 cgatccggcg gcgctcctgg tcgctgatcc gcagcacctc gttctccagg cggcggcgct
     2761 ccgagatgtc gcgaacgacc ccggtgaacg tacgggaatc ctccgtgcgg acctccgata
     2821 ccgagagctc catcggaaag acggatccgt ccctgcgctg gccgacgacc tcccgcccca
     2881 tgccgatgat cttcgcctgt cccgtctgca ggtagttcgc cacgtacgta tcgtgctcct
     2941 cgcggtacgg ctccggcatc agcaggccga ccgaccggtc gacgatctcg tccgcgcggt
     3001 atccgaagat cttctcggcc gccgagttga acgcctgcac ccggccctgt gcatccatga
     3061 cgatgattcc gtccaccgtg gtgtccagga tggtctcgag ccgcttctcg cgggcgatga
     3121 gcgccccgat cctcctgagc accaggggga gccttctgtc gagcgtctcg ccggcgaggc
     3181 acaggtcgtc cgccccggcc tcctcgtacg cgccggcggc cacggtcccc ggtgtgtact
     3241 ccaccgcgat gatgaaggcg ccctgcccca cccgcatgcg tgtccgtcga acccgcgcca
     3301 cgtcgcccgg accgcgaacc gcgacgagca cgaagtccgc ccggccacgc agcctgcccg
     3361 gttccggtac gaagcggacg ccgggacacc gcagcgcctc gggcatttcc ttctcgtccg
     3421 caccgaccac gaagaccttc atgctccctt cagccacggt tccggtcata ccggaattta
     3481 gcccacacgc cccgtagggc aaacccgccc ggtgccccgc cggttgcccc ggcaggtgga
     3541 ttcccggtga aggtctcctg cccggcggag ggcgtcagcc ggacccgcgt ggaggcggaa
     3601 gcgcctcgag cagtctcgga gtgacgagtg ccgacagccg tccaccgccg acgcggcagt
     3661 cgacccaccg gtcgacctcg aaatcaatgc gggcgagcgt tccggtcgcc atgcggacgc
     3721 tggaaccgcc ggtaagcacc cgggccgcct ccgaccaccc gggctcatgg ccgacgagca
     3781 ggatgcgatc gatgtcgtcc ggaagcgcgg ccaggattcc ggagacgtcg gccgggccgg
     3841 ccccgtagag cgaccgcagg tcctgccgcg gcacgtggtc ccagccgccg tgacgggccg
     3901 cccgggtcag ggtgtccgcc gccctcactg cggtcgacgt caggcacagg tccggaacga
     3961 ggccggcttc cgcgatgaac aggcccatcc gggcggcggc cttccttccg cgtccggaca
     4021 gcggccggtc gtggtcgtct cccgggccgg agtcccagtc ggacttcgca tggcgcagaa
     4081 gcaggaggga tttcacggcg tcagtccccg cgcgtcgagc ggtagacggc ctccgcgaag
     4141 tcgcagggtc ccccgaccgg cgggttggcc ttccggacgc ggacctccac ggacacgagg
     4201 ttgccggatt cctgcagcac ctgccgggcg atcaggtacg ccaggcgctc gatgaggtag
     4261 aaccggttgt gcgtgatcgt gtcgtggacc acccggtaca cgcgctcgta gtcgacggtc
     4321 cgcgacaggt cgtccgccgt ggcggcaccc tcgaaatcga ggtccatggc aacgtcgacc
     4381 tcgtaccggc ccccgatccg atgttcctcc tgcatcaccc cgtggtgggc gtagaagacg
     4441 gcgttgcaca ggcggacggt accgatgggc atggacgcaa ggggggtggt gtgaaggccg
     4501 ccggggcgga gccaagatac gtgcgtcaga acccgagcgc ttcgacgagg cgcgccctct
     4561 ccgccggaag gcgcccggct tccgcctccg ccctccagcg tcccaccagg acggtggcat
     4621 acaggcccag cagaccgtcg gagcgccggc gatgctcgtc gagacgggcc acgacgtccg
     4681 gatccaccca ttcgccggga acgtcgacga accggggacc gtgcggaccg tccgccgcag
     4741 gagccgggaa gctggcctcg ggctcgaacg cgcgatcgcc gtaatgacac ccgaagcagc
     4801 gggtcggcac ggcaaggccg ccccggtccc cgtggatctc cccgatcggc tcgccgccga
     4861 ctccataggc cgcgaaatcc cagtagccgt ccgcccggcg gatcatcgcg gtcgtctcca
     4921 ccacgtcacc gtcctcgagg tgctctccca cgacgacggt cccggcgggg tacctgagct
     4981 cgccctcggc agcgaatgtc tcgagcgccg agacgaccgc ggccaccgga atgcgcaccc
     5041 gcctctcgta gcgggtcatt gagccgcgaa cccttatctc gacgcgctcg tcgtccgggg
     5101 ctccgaatcc ggggagggcg acgaggtcgg tcgcgctccc cgggagcctc cgggcctcgg
     5161 cgtacgtcgc ctgcaccgcg gcacggagct cgtcccggtc cagcaacccg tccccggcgg
     5221 atggctccaa ctgcgcacgc agctccggcg cggtactcgc cagtgattcc aggtcgagcc
     5281 gccagccgtc cccgtcggca acgagcaccg aatcgatccg ggcgggatcc atgtacgagc
     5341 cgagatagaa ccggagcacc cgcccggcgt cggaccggcc cgcatcgacg gcgacgcccg
     5401 agggagccgg cgcggccgtg catcccgcca tcacgcacgc ggccagaagg aatcctctca
     5461 cgatctcgcc attcgggcct ccaccctgcg cctgatcgcc gagagcccgg gctcgggacc
     5521 gaacgatcgc tcataccggc cggcgaactc gtcgaatccg aagcgatggt tggacgtgcc
     5581 gagctgttcg agcacgtagg tcgagcagag cgcacccacg cggcccgcga ccgacagcgg
     5641 gaggtcggcg ctcaatgccg cgaagaagcc gccgcgatag gcatcgcccg cgccggtggg
     5701 ctcgacgatc tgctctggca cggcagcggg aatctcgtga atggctccgt ccgcgtggat
     5761 cgtgctgccg cgggctccgc tcgtgacgac caggcagtcg acccggccga ggatctcgtc
     5821 catggtccag ccggtcttct tcatgatgat ggccagttca tactcgttca ccatgaggga
     5881 ccgggcgccc gggatgctcc ggcgcaggtc atccccatcc agccgtgccg tctgctggct
     5941 gggatcgtac gcgaagggga ccgcggcacg gcggcattcg tccgccaggt tcagcatcgc
     6001 ctcgggatcg ttcgggctga tgatcaccag gtccaccgag tcaaggccct gatccgcgag
     6061 cgagaagcgc cgagcgtgag ccatcgcacc ggtgtagaaa ttcgcgatct ggttctggtc
     6121 caggtccgtg ctcacgaaga agctggccgt gaagtcgtcg tcgatctcga tgatggagtc
     6181 ggtcgacaga ccctgctcct cgagccaggt ccggtaatcc ccgaaatcct ggccgacggt
     6241 cgcgaagatg acgggctcgc cgccgagcag ccgcatcgtg taggcgatgt tcgccgcgac
     6301 gcccccccgc tggcgcacca tcgaatccac gagaaagctc agactgaccg actccatgcg
     6361 atccgcgatg aaatggtcct tgaagcggcc cggaaagcgc atgagataat cgtacgcgat
     6421 cgagcccgtc acggcgattc gcatggacag gcggcgctgg gaaggaaacg acgctcctac
     6481 ggagacgtcg ccgaacggat cctcggacgc ctcaccaccg attcgcgccg tcggcatacg
     6541 aagcagcgac cgtcgcgcgt tttcggtacg gccgcacgag aaccgctcac cgggtgggcc
     6601 gtttccgcgt agggtgccac gtggtggctc cgtatcttgc gccgccccct ccgtcttcac
     6661 cggacgagcc cgttctccat cctggtatgc ccgatttccg attgacgccg gtccgcatcc
     6721 tcttcagcct gatcgccctg accgcccctc tgtcgagggc cggggcacag ccggtgccgc
     6781 cgaacgagac cacgtcctat gccgaggccc tccggacctt cgaggatcga cgcttcggca
     6841 ccgcggccga gcggttcgag gcgtttcgtg accggtttcc ccggagcgtg cacgttcccg
     6901 acgccaccta ccacgaaggg gccgcgctcc tcgcgctcgg tcgcgaccag gacgcggtcg
     6961 cgctcctcac gcgcttcgag ctggacttcc cgagccaccc cctggccttc gaggcccgcc
     7021 tcgcgctcgg ccagtactac tacgacaacg gcaatgccca tgaagcgctg accaccctcg
     7081 agcaggtgct cctggggtcg ccgccggacg agacggccgc ccgcgccctc tactggatgg
     7141 gcgacgccgc ggtcgagctc gatcgcatgg aggacggcct ggaatactac cgccgcgcgg
     7201 ccgacgagta tccggaaacc gagaccgccc cgaaggccct ctatgcccgg gcgttcacgt
     7261 tcgtgagtct cgggcggtac gaggaaggag cccagacgct ggagcggctc gaggcccggt
     7321 acacgcagtc cgacctcgca cgcaacgtgg gactcgccct cgccgaggtg tactacgaga
     7381 tcggcgacta cgagcgcgcc gtcgccgaga tccggcggag gctgccgagt ctcgtccgcg
     7441 aggtcaagga ccgggcggag ttcctcctcg ccgagtcgta caaccagctg cgggattcgg
     7501 agaacgcgat cagcgcgtac cgccggttca cggagggacc cgaggagagt ccctactacc
     7561 gtcgggcggt gtacggcctc gcctggaact actaccacga gggggcgcac cagtgggcgc
     7621 acgagcagtt tgcgcgcctc gtggccggca gcgacgacct ggccatcgag gccctctatt
     7681 acgagggcgt caacctggcg atggccgccc gaggccagga cgc
//
LOCUS       DBNHQZ010000112         9106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_980826, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000112 DBNHQZ010000000
VERSION     DBNHQZ010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_980826"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="AC16YU_02855"
     CDS             <1..1049
                     /locus_tag="AC16YU_02855"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HIR6064190.1"
                     /translation="DADAVVVRSITRVDASLLEGTEVRFVGTATSGVDHVDRSWLRKQ
                     GIRLAHARGANAESVVDYVIAALLRLCARRGESLVGRTVGIVGCGNVGGRLARRLSHF
                     GTEVLLRDPPKERRALAAGRSAPYVSLEAILERSDIVTLHTPLTRHGRDATHHLVDAA
                     FLEALRPAAWLVNTARGPVVDQAALLARTEGSGALVLDVWEDEPEPDPAVVSRADIAT
                     AHIAGYALDSKFEATRRIARALARHLGRALPSARPAPEPLIPVRAPAPRRGDADWLDA
                     LTRLLYPIERDDLDLRRVVAAPEAERARGFEALRKGYPVRRLFRRHAVAPGEVPPHLR
                     GAVFGGLGLGRLPG"
     gene            complement(1054..1956)
                     /gene="era"
                     /locus_tag="AC16YU_02860"
     CDS             complement(1054..1956)
                     /gene="era"
                     /locus_tag="AC16YU_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR6064191.1"
                     /translation="MTDPVGTPSAPHRCGYATLVGRPNVGKSTLLNALVGQKISIVTR
                     KPQTTRTRVLGILTGPDFQAILLDTPGIIAPRYGLQEAMMREVTASIRDADVVLFLAE
                     AGDPAPDARSLEHVEGRRAILVVTKMDRVQAEQALPLVDAYRSLRAFEEVVPVSALKR
                     RGLETLLQVLRDRLPEGPPLYPADMLSEQPERFFVAEIIREKIFERYRQEVPYATQVN
                     VVAYEERPGEKDLIDAEIVVERDTQKGILIGKSGRALKAVGTAARIDIEALVGRPVFL
                     RLFVKVREDWRNSATHLRSFGYRS"
     gene            complement(1953..2696)
                     /locus_tag="AC16YU_02865"
                     /pseudo
     CDS             complement(1953..2696)
                     /locus_tag="AC16YU_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
     gene            2912..3373
                     /locus_tag="AC16YU_02870"
     CDS             2912..3373
                     /locus_tag="AC16YU_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064192.1"
                     /translation="MASSSQALRTALQLVLAIIIVGLAYFLYVSITEPYAAVEHRREL
                     TRMTRDRMGQVRAALGVFERANGRYPSSLDSLVLYVRTDSVVSTKTDSLFGEGFSPDS
                     LVYSPRTGSKFEYALNDTGRVAIYLLKDPDTDDHIGSLIPDVTLLNAASWE"
     gene            3455..4273
                     /locus_tag="AC16YU_02875"
     CDS             3455..4273
                     /locus_tag="AC16YU_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064193.1"
                     /translation="MAGHQFRYAVVHVGRSGNRLLRLGECSFDFDTVEVLGLHAQAAD
                     REILLDGLREALGPVPPPALRFVVHPPVATTFLSVPEGEDAETAAHRDARLLGLLDDE
                     GMTVRIDHLDGHHGVELVTLLPRDLRDAVGEVCAELGCAAFEIVPGLLAAGELMRELS
                     TDNETVVGVGVYDAGLEVVVLEGGLWRVGTSAAAVGGTRPEVFTRTLESGLFLVAERI
                     LRFRYGSVAGGEVAPGLEDHVPFDGLRVLQYDAGGVDPTTDPSLFTAAVGAAIL"
     gene            4281..4817
                     /gene="rsmD"
                     /locus_tag="AC16YU_02880"
     CDS             4281..4817
                     /gene="rsmD"
                     /locus_tag="AC16YU_02880"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6064194.1"
                     /translation="MRIIAGRLKRRTITVPRGLDVRPTSDRTREAIFSLVEARLELED
                     ADVLDLFSGSGALGFEALSRGARSAEFVEWDRRAARGIRDAAERLEVDAEATVIQADA
                     VAYLRRYSGEPYDCIFADPPYGMEGVSALPELALPHVAKGGLFVLEHDRSIRFEDEPG
                     LMTTRSYGRSTVSLFAPE"
     gene            4829..5317
                     /gene="coaD"
                     /locus_tag="AC16YU_02885"
     CDS             4829..5317
                     /gene="coaD"
                     /locus_tag="AC16YU_02885"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6064195.1"
                     /translation="MAADRLALYPGSFDPFTYGHLDIVRRAARLFERLEVTVAVNASK
                     EPLFDVAERCRLIRLCVGDLLNVTVEPFEGLLVDRARERGAVALVRGLRQVSDFDYEI
                     RMAFANRRLHGDLETVFLMTSESHALISASIVREIHQWGGDVSSFVPEPVRAALSTRR
                     PS"
     gene            5340..6542
                     /locus_tag="AC16YU_02890"
     CDS             5340..6542
                     /locus_tag="AC16YU_02890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6064196.1"
                     /translation="MTEHSFSPNVAAMQPSATLAITALAKTRRRDGHPVIGLSAGEPD
                     FDTPKPIADAAIAAIRDGFTRYTENTGMLALREGICRKLREENGIEYEPDEIVCSNGA
                     KQSVAMTVSVLCCPGDEVLIPAPYWVSYPEMARFAGAEPVVLPTRVEDGYLVTPEALE
                     RAITPRTRLLMLCSPSNPTGGVYAPEAIGALAEVLLRHPHVYVMSDEIYEHIRYDIGH
                     RSIASVRGMRERTVTVNGFSKAYAMTGWRLGYLAADRPVAKACAKLQSNYTSAPSSIT
                     QKAGIAALEMGLVPIHEMVRAFRDRRDFMLDALAALPGIRCPRPDGAFYVFPAIDALF
                     GTRTPGGAVIDSSEAFCLHLLEDHDVALVPGHAFGDPNGMRISYAASMDSIEEAMRRI
                     TKAVEGLR"
     gene            6602..7846
                     /locus_tag="AC16YU_02895"
     CDS             6602..7846
                     /locus_tag="AC16YU_02895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6064197.1"
                     /translation="MEPTPYRPIFSAPTYGAGPSPAGQRGGRDRYWLHILLFLLTLAS
                     TVFAGGQLVGRILHYEAADRWISLLGLQVSTEYLLDGLRFGGSLLLFLTVHEFGHYFT
                     ARHHGIRTSLPYFIPFPLNGIGTFGAVIRIREQVPSLRKLFDIGVAGPIAGFVVSFAV
                     LILALATLPPPDYLLDLPGHEALKIWIRERGTFPPGILRDPGVTDGMTLVVGQTPLYW
                     MLSQFFPNVPPMHEMYHYPVLFAGWIGLFFTALNLLPVGQLDGGHILYALVGPKWHGL
                     YARIFVVLLLVSGAIGFVAELDDFGRGSAVRGLFVWFVLTSVLLAYLNRILDRNLRRA
                     IPATVAIVVVAALAQVIGPAVTRIGYSGWLLWCFLIVFLIRIDHPPVLYQEPLTRGRR
                     MLAWASIVVFVLCFSIKPLYFL"
     gene            8029..8862
                     /locus_tag="AC16YU_02900"
     CDS             8029..8862
                     /locus_tag="AC16YU_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064198.1"
                     /translation="MHTGPRAPGIVALALLLAVAAGCDSAPGPEEAGLRPPVVSDLSY
                     EPGAILVDELPPESVSGGVATVPVTAEVRATDRDGDLDRVSWVLRGPVAGSAAVASGD
                     FTSAGSGLFRLEEEIGFPTAVPGRYTLVVFASDRSGRLGNDVRGVIDLLASGTPPAIT
                     GVEMPERVTRPAAGQPPVPVVIVVTASDPDGLANILRVETRVDGGAALALCDDGGEGA
                     CNPAVTQAPSGDQVAGDGRFTVTLQVEASNSAGTRTFTFEAVDRSGLRSAVVERTLVI
                     E"
     gene            8859..>9106
                     /locus_tag="AC16YU_02905"
     CDS             8859..>9106
                     /locus_tag="AC16YU_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064199.1"
                     /translation="MTKAAFPIALLLAAAAAAPARAQSGGGPVESEFGILSNSIANLD
                     VAGDTVWAGPYLSMTPDGGATWLVAGTDSLFGTSNRVFS"
BASE COUNT         1241 a         3176 c         3189 g         1500 t
ORIGIN      
        1 gggacgcgga tgcggtcgtt gtacgcagca tcacccgtgt ggatgcatcg cttctcgagg
       61 gaacggaggt ccggttcgtg ggaacggcta cgagcggcgt cgatcacgtg gaccggagct
      121 ggctccggaa gcaggggatt cgtctggctc acgcccgggg ggccaacgcg gaatcggtgg
      181 tcgattacgt tattgcggcc cttctccgcc tctgtgctcg gcgcggagag tccctcgtgg
      241 gccgcacggt cggcatcgtg ggatgcggaa acgtcggggg gcgcctggcg cggcgtctgt
      301 ctcatttcgg aacggaggtt ctgctgcggg atccgccgaa ggagcgcagg gccctggcgg
      361 ccggccgctc cgcgccgtac gtgtccctgg aggcgatcct ggagcggtcg gacatcgtca
      421 ccctgcacac gccgctcaca agacacggaa gggacgccac gcatcacctc gtcgatgcgg
      481 ctttcctgga ggcgcttcgg cccgcggcgt ggctcgtcaa caccgcgcgg ggcccggtcg
      541 tcgaccaggc ggcccttctc gcccggacgg aggggtccgg cgcgctggtc ctggacgtgt
      601 gggaggacga gcccgagccc gatcccgcgg tcgtgtcgcg ggcggacatc gcgaccgccc
      661 acatcgccgg ctacgcgctg gattcgaagt tcgaggccac gcgccggatc gcccgcgccc
      721 tggcgcgcca cctcggacgc gcgctccctt cagcccggcc ggctccggag ccgctcattc
      781 ccgtccgggc tcccgcgccc aggcggggag acgccgactg gctcgacgcg ctgacccggc
      841 tcctgtaccc gatcgagcgc gacgatctgg acctgagacg cgtcgtcgcg gcgccggaag
      901 cggaacgcgc ccgcggcttc gaggcgctgc ggaagggcta cccggtgcgg cggctcttcc
      961 gccgccatgc cgtggctccc ggcgaggtgc ccccccacct gcggggagcg gttttcgggg
     1021 gcctcgggct tggccggctt ccgggttgag aggtcaggaa cggtagccga acgagcgcag
     1081 gtgggtcgcc gaattgcgcc agtcctcgcg gaccttcacg aacaggcgca ggaagacggg
     1141 ccggccgacg agggcctcga tgtcgatccg cgcggccgtc cccaccgcct tcagggcccg
     1201 ccccgacttc ccgatcagga tgcccttctg cgtgtcgcgc tcgacgacga tctcggcgtc
     1261 gatgagatcc ttctcgcccg gcctctcctc gtacgccacg acgttcacct gcgtggcgta
     1321 gggaacctcc tgccggtacc gctcgaagat cttctcccgg atgatctccg cgacgaagaa
     1381 gcgctcgggc tgctcggaga gcatgtccgc tggatacagc ggcggcccct cgggaagccg
     1441 atcgcggagc acctggagca gggtctcgag gcctcggcgc ttcagggccg acacgggcac
     1501 cacctcctcg aaggcccgga gggagcggta ggcgtcgacg agcggcaggg cctgctccgc
     1561 ctgcacacgg tccatcttgg tgaccaccag gatggcacgc ctgccctcga cgtgctcaag
     1621 gcttcgcgcg tccggggccg gatctcccgc ctctgcgagg aacaggacca cgtcggcgtc
     1681 gcggatcgag gccgtgacct cgcgcatcat ggcctcctgc agtccgtacc ggggcgcgat
     1741 gatgccgggc gtgtccagga ggatcgcctg gaaatccggg cccgtgagga tgcccagcac
     1801 gcgggtgcgg gtggtctgcg gcttccgggt gacgatggag atcttctgac ccaccagggc
     1861 attgagcagg gtgctcttgc ccacgttggg tctccccacg agcgtcgcat acccgcagcg
     1921 gtgcggcgct gacggcgtcc cgacgggatc ggtcatcggg gtgcgggcca cttgaccagg
     1981 gcggccatgt cccgcacggc ctgctccagc ccgacgaaca gagcgcgggc gacgatcgcg
     2041 aagccgatcg acacctcgtc cacgtccggc agggccgttc agaacgtcgg gtagttcagg
     2101 taatccaggc cgtgcccggc gtgcaggccg aggcctgcct cgcgcgcggc gcgacccgcc
     2161 tcggccagcc tcaggcattc gcgacggacg tcctccgcgg tgcgggccag cgcgaaatcg
     2221 ccggtgtgaa gctccaccgc gttggcgccc gcctcggcgg cggcgtggat ctgcagcggg
     2281 tcgggatcca cgaagagcgc gacctcctcg atgccggcgt cgagcagccg cgcaatcgcg
     2341 ctgtgcaggc gctctccgcc cgccgggacg tccaggccgc cctcggtggt gacttcctcc
     2401 cggcgctcgg ggacgagggt ggccagctcc ggacggatgc cgcagcagta ctcgatgatc
     2461 tccgggctca ccgagagctc gaaatccagc ctggaacgga ccacctcgcg aaggcggcgg
     2521 acatcgtcgt cgttgatgtg gcggcggtcc tccctgaggt ggaacacgat gccgtcggcg
     2581 cccccgagct cacactggac ggcggccgcg acggggtcgg ggaacacctc gcgccgcgcg
     2641 ttccggagcg tggcgacgtg gtcgatgttg atgaggagtc gggtcgtggc tgccatgccc
     2701 gtcaggattc aggggccgcg cggcccgggg ccgagcgctc ttgcgctgcc gtgaacgtac
     2761 gccgcggacc tttgtttttc cgtcgcccgg cgggaatccc cgggaggccc tgcagattgc
     2821 atttcgcggg gtgcgccagt aacttcgtcg cctgtccgcg gtcaccaagt ccgcaggctt
     2881 ccgtcgccaa ccattcattc cactccgcgc gatggcttcc agcagccagg cattgcgaac
     2941 cgccctccag ctcgttctcg cgatcatcat cgtcgggctg gcctacttcc tgtatgtctc
     3001 gatcacggag ccgtacgcgg cggtcgagca ccggagggag ttgacccgga tgacccgcga
     3061 tcgcatgggc caggttcggg ccgccctggg cgtcttcgag cgggcgaacg gccggtatcc
     3121 ctcttccctg gactccctgg tactgtacgt gcggacggat tccgtcgtct ccacgaagac
     3181 ggacagcctg tttggcgaag gattcagtcc ggactcgctc gtgtactcgc cgaggaccgg
     3241 ttcgaagttc gagtacgcat tgaacgacac gggccgggtg gccatctacc tgctcaagga
     3301 tccggatacc gacgaccaca tcggctcgct gatccccgac gtgacgctgc tcaacgcggc
     3361 gagttgggag tagagccccg attcggcgtc cctcgccggg cgtgggtgga cgtcccagcc
     3421 gcgcatggcc cttcccgcac gagccggtat cgacttggcc ggacatcagt tccggtacgc
     3481 ggtcgtgcac gtgggacgat cgggcaaccg gctcctgcgg ctgggcgagt gctcgttcga
     3541 ttttgacacc gtcgaggtgc tgggtctcca cgcccaggcc gcggaccggg aaatcctgct
     3601 ggacggcctt cgcgaggccc tcggtccggt gccgccgccg gcgctccggt tcgtcgtgca
     3661 tccaccggtc gcgacgacct ttttgagcgt accggagggc gaggatgcag agaccgcggc
     3721 gcaccgcgac gcgcgtctcc tgggactgct cgacgacgag ggcatgaccg tgcggatcga
     3781 ccacctggac ggccatcacg gggtggagct cgtgacgctg cttccccgcg acctgcgcga
     3841 cgcggtcggc gaggtgtgcg cggagctggg gtgcgcggcc ttcgagatcg ttcccgggct
     3901 cctcgccgcg ggcgagctca tgcgggagct gtccacggac aacgagaccg tggtgggcgt
     3961 cggggtgtac gacgcgggcc tggaggtcgt cgtgctggag ggcggcctgt ggagggtcgg
     4021 tacttcggct gccgcggtcg gcggcacgcg gccggaggtc ttcacgcgga ccctggagtc
     4081 cgggctgttc ctcgtcgcgg agcgcatcct ccggttccgt tacggctctg tggccggcgg
     4141 cgaggtagcg ccgggcctcg aggatcacgt gccgttcgac gggctgcgcg tcctgcagta
     4201 cgatgcgggc ggggtcgacc cgaccacgga tccgagcctg ttcacggcgg cggtgggcgc
     4261 ggccatcctg tgaggacgcg atgaggatca ttgccggccg cctgaaacgg aggaccatta
     4321 ccgttccccg gggcctcgac gtccgtccga cgtccgaccg cacgcgcgag gccatattct
     4381 cgctggtgga ggcccgcctc gagctcgagg acgcggacgt gctcgacctg ttctccgggt
     4441 ccggcgcgct cggcttcgag gccctgagcc ggggcgcccg atcggccgaa ttcgtcgaat
     4501 gggaccgccg ggccgcccgg ggcatccggg acgccgccga gcgtctcgag gtcgacgcgg
     4561 aagcgaccgt gatccaggcg gacgccgtcg cgtacctgcg ccggtattcc ggcgagccgt
     4621 acgattgtat attcgccgac ccgccgtacg ggatggaggg tgtctcggca ctgccggagc
     4681 tggcccttcc gcacgtcgcg aaagggggtc tgttcgtgct ggaacacgac cggtcgatcc
     4741 ggttcgagga cgagccgggc ctcatgacca cccggtcgta cggcaggagc acggtttcgc
     4801 ttttcgcacc cgaatgatcc gccaggccat ggcagccgac cgcctcgccc tgtacccggg
     4861 ctccttcgat ccgttcacct acgggcacct ggacatcgtc cggagggccg cgcggctgtt
     4921 cgagcggctc gaggtgacgg tcgctgtgaa cgcgtcgaag gagccactct tcgacgtggc
     4981 cgagcgctgc cggctgatcc ggctgtgcgt gggagacctc ctgaacgtga cggtcgagcc
     5041 cttcgaaggc ctcctcgtgg accgggcgcg cgagcgcggg gccgtggcgc tggtccgggg
     5101 gctccggcag gtgagcgact tcgactacga aatccggatg gcgttcgcga accgtcggct
     5161 gcacggggac ctggagacgg tcttcctcat gacgtccgag tcccacgcgc tgataagcgc
     5221 ctccatcgtc cgcgagatcc accagtgggg gggcgacgtg tcctccttcg taccggaacc
     5281 ggtgcgggcg gcgctgtcta cccggcggcc gtcctgatcc gatcatcgat gcacatcccg
     5341 tgaccgagca ttccttcagc cccaacgtgg cggcgatgca gccatccgcc acgctcgcga
     5401 taaccgcgct cgccaagacc cggcgtcgcg acgggcatcc ggtcatcggc ctgagcgccg
     5461 gcgagcccga cttcgacacg ccgaaaccga tcgccgacgc ggcgatcgcg gcgatccggg
     5521 acggattcac gcggtacacc gagaacacgg gcatgctcgc gctgcgcgag ggcatctgcc
     5581 ggaagctccg ggaggagaac ggcatcgagt acgagccgga cgagatcgtc tgctcgaacg
     5641 gcgcgaagca gtcggtggcg atgaccgttt cggtactgtg ctgtcccgga gacgaagtcc
     5701 tgatcccggc gccgtactgg gtgagctatc cggagatggc acgcttcgcg ggcgccgagc
     5761 ccgtggtgct ccccacccgc gtcgaggacg gctacctggt gactcccgag gcgctcgaac
     5821 gggccatcac gccgaggacc cggctgctga tgctgtgctc gccgtcgaat ccgacgggtg
     5881 gcgtctatgc cccggaggcg attggcgcgc tggcggaggt cctgcttcgc catccgcacg
     5941 tgtacgtgat gtcggacgag atctacgagc acatccggta cgacatcggg caccgctcga
     6001 tcgcctccgt tcggggcatg cgggagcgga ccgtcacggt gaacggcttc tccaaggcgt
     6061 acgccatgac cggctggcgg ctgggctacc tggccgccga ccgtccggtg gcgaaggcct
     6121 gcgccaagct gcagtccaat tacacgtcgg cgccgtcgag catcacgcag aaggcgggga
     6181 tcgccgcgct cgagatgggc ctcgtgccga tccacgagat ggtcagggca ttccgggacc
     6241 gccgggactt catgctggac gcgctcgcgg cgcttcccgg cattcgttgc ccgcgcccgg
     6301 acggggcgtt ctacgtgttt ccggcgatcg atgccctgtt cgggacccgc acgccgggcg
     6361 gcgccgtcat cgactcgtcg gaggccttct gcctgcacct gctcgaggat cacgacgtgg
     6421 ccctggttcc gggccacgcg ttcggggacc cgaacggcat gcgcatttcg tacgccgcct
     6481 ccatggacag catcgaggag gccatgcgcc ggatcacgaa ggccgtcgag ggcctgcgct
     6541 gaacgttccg gcgcggtccc ggcaccgaac aatccacccc tggacgcgta cgcgcggcgc
     6601 catggagccc accccgtacc gcccgatttt ctccgcgccc acctacggcg ccggaccgag
     6661 cccggccggg cagcgcggag gccgcgaccg gtactggctc cacatcctgc tcttcctgct
     6721 cacgcttgcg agcaccgtgt tcgccggcgg gcagctcgtg ggccggatcc tgcactacga
     6781 ggcggccgac cgctggattt cgctcctcgg gctgcaggtc agcacggaat acctgctcga
     6841 cggtctccgc ttcggcggct cgctcctgct gttcctgacc gtgcacgagt tcgggcatta
     6901 cttcacggcc cgtcaccacg gcatccggac ctcgctgccc tacttcatcc cgttcccgct
     6961 gaacggcatc ggaacgttcg gcgcggtgat ccgcatccgc gagcaggtcc cgtcgctccg
     7021 gaagctcttc gacatcgggg tggccgggcc gatcgcggga ttcgtggtct cgtttgccgt
     7081 gctgatcctg gccctcgcga ccctgccgcc gcccgactat ctcctggacc tgcccggcca
     7141 cgaggccctc aagatctgga tccgggagcg cgggacgttt cccccgggaa tcctgcgcga
     7201 tcccggggtc acggacggga tgacgctcgt cgtcggccag acgccgctgt actggatgct
     7261 cagccagttc tttccgaacg tgcccccgat gcacgagatg taccactatc ccgtgctgtt
     7321 cgcgggctgg atcggactgt tcttcacggc gctgaacctg ctgccggtgg gccagctcga
     7381 cggggggcac atcctttatg cgcttgtcgg cccgaaatgg cacggcctgt atgcccgcat
     7441 cttcgtcgtc ctgctgctcg tttcgggcgc gatcggattc gtggccgagc tcgacgactt
     7501 cggacggggc tccgcggtgc gcggcctgtt cgtgtggttc gtgctcactt cggtcctcct
     7561 cgcgtacctg aaccggatcc tggaccggaa ccttcgccgg gcgataccgg cgaccgtggc
     7621 catcgtcgtc gtggcggcgc tggcccaggt catcgggcct gccgtcacgc ggatcggcta
     7681 ctccgggtgg ctcctgtggt gcttcctgat cgtgttcctg atccgcatcg accatccgcc
     7741 ggtcctgtac caggagccgc tcacgcgcgg acgccgcatg ctggcctggg cctcgatcgt
     7801 cgtgttcgtc ctgtgcttct cgatcaagcc gctgtacttc ctgtgaccgg aagagcacgc
     7861 atcacgacgg acgagccacg tgggcgcatc ggcgcccccg cgtttgcacc cgatcacccg
     7921 ggctgtatct tcggccctgc cacgacagcg agcccccgcg cgccgaccga tccgaacgct
     7981 acgatccttc cttccgaacg catccgtcgg atgaaacgcg gatctgtcgt gcataccggc
     8041 ccccgggctc ccggaatcgt ggcgctcgcc ctgctcctgg cggtcgccgc cgggtgcgac
     8101 tccgcgcccg gtcccgagga ggcgggcctc cggccgcccg tcgtgtccga cctctcgtac
     8161 gagcccggag ccatcctcgt cgacgagctg cccccggaga gcgtctccgg aggcgtggcc
     8221 accgtgcccg tgacggccga agtccgggcg acggaccgcg acggcgacct ggaccgggtg
     8281 tcgtgggtgc tgcgtggccc cgtggcgggc tccgcggccg tcgcgagcgg ggatttcacg
     8341 tccgccggga gcgggctctt ccggctggag gaggagatcg gctttcccac ggccgtgccg
     8401 ggacggtaca cgctcgtcgt cttcgcctcc gaccggtccg gccgcctggg caacgacgtg
     8461 cgcggggtga tcgacctcct ggcgagcggc acgccgcccg cgatcaccgg cgtggagatg
     8521 cccgagcggg tcacgcgccc cgccgccggt cagccgcccg ttccggtggt catcgtggtt
     8581 accgcctcgg atccggacgg cctggccaac atcctgcgcg tcgaaacccg ggtcgacggc
     8641 ggcgccgccc ttgctctgtg cgacgacggc ggggaaggag cctgcaaccc tgccgtcacg
     8701 caggcgccga gcggcgacca ggtggcgggc gacgggcggt tcacggtgac gctccaggta
     8761 gaagcgtcga attccgcggg cacgaggacg ttcacgttcg aggcggtcga ccgctccggg
     8821 ctgcgcagcg ccgtcgtgga acggaccctg gtgatcgaat gacgaaggcc gcgttcccca
     8881 tagcgctcct cctggccgcc gcggccgcgg cgcccgcccg cgcgcagtcg ggcggcgggc
     8941 ccgtcgagtc cgaattcggc atcctctcga actccatcgc gaacctggac gtggccggcg
     9001 acacggtgtg ggcggggccg tacctgagca tgacgcccga cgggggcgcg acgtggctgg
     9061 tcgccggcac ggattccctg ttcggcacgt cgaaccgtgt cttctc
//
LOCUS       DBNHQZ010000113         7362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1115581, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000113 DBNHQZ010000000
VERSION     DBNHQZ010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1115581"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="AC16YU_02910"
     CDS             <1..952
                     /locus_tag="AC16YU_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR6064200.1"
                     /translation="GVRFLRTTTGEQLIPFDELTPDDLVIVPAFGATMETLDLLRGRG
                     IDVETWDTTCPFVEKVWKRSEQIGREDYTIIIHGKRAHEETRATFSHAKSVAPVVVVR
                     DLAEAAELASFIRGERDPAGFSDAFPDRTSPGFDPARDLVRVGVVNQTTMLATETEAI
                     AALLRSAMRDRYGEAFLAHHFADTSDTLCYATNENQAATMALLEADAHCALIVGGFNS
                     SNTSHLVELSEEHLPSYFIGSRDDILSRGRIRHFDFRARALRETADWLPPDRPVDVLL
                     TAGASCPDILLDQVIGRILSFEEDVRPVDDVIEALAVAHP"
     gene            949..1521
                     /locus_tag="AC16YU_02915"
     CDS             949..1521
                     /locus_tag="AC16YU_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064201.1"
                     /translation="MTGRRLLAAALLGGLALAGCSLLGTRDPEDPLAEGGTYLQPDTP
                     EQVVDNLRAAIAELNTLNYRRSLSEALEFRPTATAEARDAVWSTWSRAEEEQYFGTLV
                     AAAAGNTGHGLVLNDESFTLVDASHYRLDATYVLTVIHTRPDAPTRVQGRLLWTIEQR
                     TDGLWELSAWTDQELGTEPSWSDLKAEFVK"
     gene            1536..3026
                     /gene="xylB"
                     /locus_tag="AC16YU_02920"
     CDS             1536..3026
                     /gene="xylB"
                     /locus_tag="AC16YU_02920"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR6064202.1"
                     /translation="MSVFLGLDISTTATKALLVDESGRVVGSAASPHRLLTPRPLWSE
                     QDPEEWWAATSHAVKGVLRDTGTPPEAVAAIGLAGQMHGLVLLDADGRVLRPAILWND
                     QRASAECEEIRLRMGGLQALVAATGNDAFPGFSAPKLLWVRRHEPGIYGRVRQVLLPK
                     DYVRFRMTGAYATDRAGAGGTLFLDLATRDWSDRVLQALDVPRELLSATHEGTEITGL
                     LDAGAGGALGLPAGIPVVGGGGDQAAQAVGVGAVTPDVSALTVGTSGVVFAASERPVF
                     DPDGRAHAFPHAAPGLWHMMGVMLSAAGSLQWYRDTLAPDASFDDLVGEAAGVPPGSD
                     DLLFLPYLSGERTPHADPRARGVFFGLTLSHGRGHLTRAVLEGVAFGLKDNLDLLVRA
                     GVPAPSALRASGGALKSATWRGILASVLDTDLEIVASPEGAAQGAALLAGVGAGAWGD
                     VRDACRAAVRIDRVEHPDPESAGRYREVHARFRALYPAVKEHFPTG"
     gene            3049..3561
                     /locus_tag="AC16YU_02925"
     CDS             3049..3561
                     /locus_tag="AC16YU_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064203.1"
                     /translation="MPIRRTIPGLLLAALLAGCNPFAPALEEGSAFGDLLGDPTTVEG
                     FFTNFRNAYELRDLSLYEPLLDSQFVFIWRDFDAQIDREWGFAQDLETTRRLFANAGL
                     IRLQWNQILSTDEIVAQRQTRVIRSFNLTITLETGDVFRGDGNVNFLLARRDSTAAWK
                     LLRWRDESEF"
     gene            3700..5073
                     /locus_tag="AC16YU_02930"
     CDS             3700..5073
                     /locus_tag="AC16YU_02930"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR6064204.1"
                     /translation="MDRALQYAEEHFETFVSELEDLLRIPSISTDPEYAGEVRRAAGW
                     LVDHLKALGFPVAEAIDTEGHPIVVAEYIVDPSLPTVLVYGHYDVQPPDPLDLWNSPP
                     FEPVRRNGLLYGRGTCDDKGQLFMHVKAAEAWLKTVGRLPINLKFMFEGEEENGSTHL
                     PGFIRAHRERLAADVVVVSDTTLFGPNIPSITYGLRGLAYVEVTLTGPDRDLHSGVYG
                     GAVENPINALASLIAGLHDEHHRVTIPGFYDNVRPLTDQERSNFAALPFDEAEWAGEV
                     GVAVTKTEDGYTALEGTTARPTLDCNGIWGGYIGKGAKTVLPSKASAKISMRLVADQT
                     PAEITDKLRRYLEAHTPPTMKLTFTDLHGGYGVLVDLDSPAMEAAAEALRGTYGSDPV
                     FIRSGGSIPVVADFKNILGLDTVLMGFGLDSDSIHSPNECFGLDRFQQGVSTIIRFLE
                     AYARRGA"
     gene            5076..5795
                     /locus_tag="AC16YU_02935"
     CDS             5076..5795
                     /locus_tag="AC16YU_02935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6064205.1"
                     /translation="MTSHEPVCLITGASRGLGRYTALCFAEAGYRVAVCGRTEADLVG
                     VARDIRDVGGWAMHAALDVRDFDAVTAFVRKVEDACGRIDVLVNNAGLLSVYRPLAEW
                     TREQMDETLDVSLKGTMYASRAVLPGMIARGRGHIINVSSDVGRRPVPNMAPYVAAKH
                     AIVGFSGSLYREVREQGIKVTVVLPGNIDTFFGGAKPGDRDLHWSLKPEDVADVLLSI
                     ARQPGNLAQDEVILHALGQEI"
     gene            complement(5808..6182)
                     /locus_tag="AC16YU_02940"
     CDS             complement(5808..6182)
                     /locus_tag="AC16YU_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064206.1"
                     /translation="MILGILLGGLVAVAVVVIVHELFPMPGLPDGQAPDANDPVPLTE
                     YLKFPVAFTLGSLLGAAAAGRLARRKWQPAVVTVGAVMLVAAGFVMLQYPHPLWAWVA
                     GVVAPIPAAWIGGLMTHNLVVR"
     gene            6497..7297
                     /gene="mddA"
                     /locus_tag="AC16YU_02945"
     CDS             6497..7297
                     /gene="mddA"
                     /locus_tag="AC16YU_02945"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanethiol S-methyltransferase"
                     /protein_id="HIR6064207.1"
                     /translation="MRRTAVVLYGVVSYAVFLVAFLYAIGFTGNVLVPKSIDSGPAYP
                     LGDALLINLLLLGLFAIQHTIMARPVFKSWFTRFVPREVERSTFVLVASLLLLLLYWK
                     WRAVDGIVWDVSGTMGGTVLTALFWIGWATVLVSTFLIDHFDLFGLRQVYLHYTGQAY
                     HHPPFVTRGLYRFVQHPIMIGFIVGFWATPTMTVGHLLFAVVTTAYIFVGVLFEERDL
                     TKMLGEPYAAYRRRVPRFVPFLKGPGSVAQAGGAEPAMAPGPVRTQDH"
BASE COUNT         1103 a         2654 c         2470 g         1135 t
ORIGIN      
        1 gggcgtgcgc tttctccgca cgacgaccgg tgagcagctg atcccgttcg acgagctcac
       61 gcccgacgac ctggtcatcg tccccgcgtt cggcgcgacg atggagaccc tggacctcct
      121 gcgcgggcgg ggcatcgacg tcgagacgtg ggacacgacc tgcccgttcg tcgagaaggt
      181 ctggaagcgc tccgagcaga tcggccgcga ggactacacg atcatcatcc acggcaagcg
      241 cgcgcacgaa gagacgcggg cgaccttctc ccacgcgaag tccgtggccc cggtcgtcgt
      301 cgtgcgcgac ctcgccgagg ccgccgagct cgcctccttc atccgtggag agcgtgatcc
      361 ggcgggcttc tccgacgcct tccccgaccg cacgtcgccg ggcttcgacc ccgcgcgcga
      421 cctggtccgc gtcggggtcg tgaaccagac cacgatgctg gcgacggaga cggaagcgat
      481 cgcggcgctc ctccgctccg ccatgcggga ccggtacggc gaggccttcc tcgcccacca
      541 cttcgccgac acgtccgaca ccctctgcta cgcgacgaac gaaaaccagg cagcgaccat
      601 ggctctcctc gaggcggacg cgcactgcgc gctcatcgtg gggggcttca actcctcgaa
      661 cacgagccac ctcgtggagc tgagcgagga gcacctgccg tcgtacttca tcggcagccg
      721 ggacgatatc ctctcgcgcg gccgcatccg gcacttcgac ttccgcgcca gggcgctccg
      781 ggagacggcg gactggttgc ccccggaccg gccggtggac gtgctcctga ccgccggcgc
      841 gtcgtgcccg gacatcctcc tggaccaggt catcggtagg atcctctcgt tcgaggagga
      901 cgtgcggccg gtcgatgacg tgatcgaggc cctggcggtg gcccacccgt gacggggcgc
      961 cgcctgctcg ccgccgccct cctcggcggt ctcgcgctcg cggggtgcag cctgctcggc
     1021 acgcgtgatc ccgaggatcc cctggccgag gggggcacct acctgcagcc cgatacgccc
     1081 gagcaggtcg tcgacaacct ccgcgcggcg atcgcggagc tgaacacgct caactaccgg
     1141 cgctctctct ccgaggcgct cgagttccgg ccgacggcga cggccgaggc ccgcgacgct
     1201 gtgtggagca cctggagccg ggccgaggag gagcagtact tcggcaccct cgtcgcggcc
     1261 gccgccggaa acacggggca cggcctggtg ctgaacgacg agtcgttcac gctggtggac
     1321 gcctcgcatt accgcctgga cgcgacgtac gtcctgacgg tcatccacac gcgtcccgac
     1381 gcaccgaccc gcgtgcaggg gcgcctgctc tggaccatcg agcagcgcac ggacggcctg
     1441 tgggagctct ccgcatggac cgaccaggag cttggaacgg agccctcctg gagcgacctt
     1501 aaggccgaat tcgtcaagta gcgcccgcct ccgccatgtc ggtgttcctg ggtctggaca
     1561 tctcgacgac cgccacgaaa gccctcctgg tcgacgagag cggccgcgtg gtcggctcgg
     1621 ccgcgtcccc gcatcgcctg ctcacgccgc gtccactgtg gagcgagcag gacccggagg
     1681 agtggtgggc ggccacgtcg cacgccgtga agggcgtcct tcgcgataca ggcacgccgc
     1741 ccgaggcggt cgcggcgatc ggcctggcgg gccagatgca cggcctggtt ctcctcgacg
     1801 cggacggccg ggtcctgcgc cccgccatcc tctggaacga ccagcgggcc tccgccgagt
     1861 gcgaggaaat ccgcctgcgg atgggcgggc tccaggcgct cgtcgcggcg acgggcaacg
     1921 acgcctttcc cggcttctcc gcgcccaagc tcctgtgggt ccgccgccac gagcccggga
     1981 tctacggccg cgtgcggcag gtgctgcttc cgaaggacta cgtccgcttc cggatgaccg
     2041 gcgcctacgc gaccgaccgc gcgggcgcgg gcggcacgct gttcctggat ctcgccaccc
     2101 gcgactggtc cgaccgcgtg ctgcaggcgc tcgacgtccc gcgcgagctg ctctccgcga
     2161 cccacgaagg caccgagatc accgggctgc tggacgccgg ggcgggcggc gccctgggcc
     2221 ttcccgccgg catcccggtc gtgggcggcg gcggcgacca ggccgcgcag gccgtgggcg
     2281 tcggggccgt tacgcccgac gtctcggcgc tcaccgtggg cacatccggg gtggtcttcg
     2341 ccgcgagcga gcgcccggtc ttcgatccgg acggccgcgc ccacgccttc ccccacgcgg
     2401 cacccggcct gtggcacatg atgggcgtca tgctctcggc cgccggcagc ctgcagtggt
     2461 accgcgacac gctcgctccg gacgcgtcgt tcgacgatct cgtgggcgag gcggccggcg
     2521 tacccccggg atccgacgat ctgctcttcc tgccgtacct ctcgggcgag cgcacgccgc
     2581 acgccgatcc gcgcgcacgc ggcgtcttct tcggattgac cctgagccac ggccgcgggc
     2641 acctgacgcg ggccgtgctc gagggcgtcg ccttcgggct gaaggacaac ctggacctgc
     2701 tggtccgggc cggcgtgccc gcaccgtcgg cgctccgcgc gtcagggggc gccctgaaga
     2761 gcgcgacctg gcgcggaatc ctcgcgagcg tcctcgacac cgacctcgag atcgtggcgt
     2821 cccccgaggg cgcggcccag ggcgccgccc tgctggccgg cgtcggcgcc ggggcgtggg
     2881 gcgacgtgcg ggacgcgtgc cgcgcggcag tccgcatcga ccgggtggag catcccgacc
     2941 cggagtccgc cggacggtac cgggaggtcc acgcccgctt ccgcgccctg taccccgccg
     3001 tgaaggagca tttcccgacc ggatagctcc tctcgatcca tcttccacgt gcccatccgc
     3061 cgaacgattc ccgggctgct cctcgccgcg ctcctcgccg gctgcaatcc gttcgccccg
     3121 gcgctcgagg agggatcggc gttcggggat ctcctgggcg acccgacgac cgtcgagggc
     3181 ttcttcacca acttccggaa cgcgtacgag ctgcgcgacc tgtccctgta cgagccgctg
     3241 ctcgactcgc agttcgtttt catctggcgg gacttcgacg cacagatcga ccgagagtgg
     3301 ggcttcgccc aggatctcga aaccacccgc cggctcttcg ccaacgccgg gctgatccgc
     3361 ctgcagtgga accagatcct gtcgaccgac gagatcgtgg cccagaggca gacccgcgtg
     3421 atccgctcct tcaacctcac gatcacgctc gagaccgggg acgtcttccg gggggacggc
     3481 aacgtgaact tcctcctggc ccggcgcgat tcgacggcgg cgtggaagct gctccgctgg
     3541 cgggacgaga gcgagttctg aggcggaagc gcgccttctc ccggcgcgcc gagggctcgc
     3601 ccgcgggcac tgaatgaagc cttcgtgtga accgccccga gggttgctct tgcgggcatg
     3661 gcgtgtacat tatccgcatt gatccaacct gcacccgtca tggaccgcgc cctccagtac
     3721 gccgaagagc acttcgaaac gttcgtgtcc gagctcgaag atctgctccg cattccgtcc
     3781 atcagcaccg atcccgagta cgcgggagag gtccgccggg cggccggctg gctggtcgat
     3841 catctcaagg cgctcggctt cccggtcgcc gaggcgatcg acaccgaggg ccacccgatc
     3901 gtcgtcgccg agtacatagt cgatccgtcc ctcccgacgg tcctcgtcta cgggcactac
     3961 gacgtgcagc cccccgaccc gctcgacctc tggaactcgc cgcccttcga gcccgtccgc
     4021 aggaacggcc tgctctacgg ccgcggcacg tgcgacgaca agggacagct gttcatgcac
     4081 gtgaaggcgg cggaggcctg gctgaagacc gtgggccggc tgcccatcaa cctcaagttc
     4141 atgttcgagg gcgaggagga gaacggctcg acccacctgc cgggattcat ccgtgcccac
     4201 cgcgagcgtc tcgccgctga cgtcgtggtc gtgtccgaca ccacgctgtt cggcccgaac
     4261 atcccctcga tcacgtacgg gctccgcggc ctggcctacg tcgaggtcac gctcaccggc
     4321 cccgaccgcg acctgcactc gggcgtctac ggcggcgccg tcgagaaccc gatcaacgcg
     4381 ctggcgagcc tgatcgcggg cctgcacgat gaacaccacc gcgtgacgat ccccgggttc
     4441 tacgacaacg tgcgtccgct cacggaccag gagcggtcga acttcgcggc cctgcccttc
     4501 gacgaggcgg agtgggccgg cgaggtgggc gtcgcggtga cgaagaccga ggacgggtac
     4561 accgccctcg agggcaccac cgcgcggccg accctcgact gcaacggcat ctggggcggc
     4621 tacatcggga agggcgccaa gaccgtcctt ccgtcgaagg cgagcgccaa gatctcgatg
     4681 cggctcgtcg ccgaccagac cccggccgag atcaccgaca agctccgccg ctacctcgag
     4741 gcccacaccc cgcccacgat gaagctcacg ttcaccgacc tgcacggagg gtacggggtc
     4801 ctcgtggatc tggacagccc cgcgatggag gccgccgccg aggcgctgcg cggcacgtac
     4861 ggctccgacc ccgtgttcat ccggtcgggc ggctccattc cggtggtcgc cgatttcaag
     4921 aacatcctgg gtctggacac cgtgctgatg gggttcggcc tcgactcgga ctccatccac
     4981 tcgccgaacg agtgcttcgg gctggaccgg ttccagcagg gcgtctcgac catcatccgc
     5041 ttcctcgaag cctacgcccg ccgcggcgcc tgaccatgac ctcgcacgag ccggtctgcc
     5101 tgatcacggg cgccagtcgc ggcctgggcc gctacacggc gctgtgcttc gcggaggccg
     5161 gttatcgcgt ggccgtatgc ggccgaacgg aggccgacct cgtgggtgtc gcgcgcgaca
     5221 tccgggacgt gggcggctgg gcgatgcacg cggcgctgga cgtccgggac ttcgatgccg
     5281 tcacggcctt cgtgcggaag gtcgaggacg catgcggacg gatcgacgtg ctcgtcaaca
     5341 atgccggcct tctgtcggta taccgtccgc tcgccgagtg gacgcgcgag cagatggacg
     5401 agacgctgga cgtcagcctg aagggaacga tgtacgcgtc gcgggccgtc ctccccggca
     5461 tgatcgcgcg cggacggggc cacatcatca acgtctcgtc ggacgtcggc cgccgtccgg
     5521 tgcccaacat ggcgccctac gtcgccgcga agcacgcgat cgtcggcttc tccggatccc
     5581 tgtatcgcga ggtgcgcgag cagggcatca aggtgacggt cgtgctcccc ggcaacatcg
     5641 acacgttctt cggcggggcc aagcccggcg atcgcgatct gcactggtcc ctgaagccgg
     5701 aggacgtcgc cgacgtgctg ctctccatcg cccggcagcc gggcaacctg gcgcaggacg
     5761 aggtcatcct gcacgcgctc gggcaggaga tctgacggcc ggcaggctta ccgcaccacc
     5821 aggttgtgcg tcatgaggcc gccgatccac gccgcgggaa taggcgcgac caccccggcc
     5881 acccaggccc agagcggatg gggatactgg agcatgacga agccggcggc gaccagcatg
     5941 accgccccga ccgtgacgac cgccggctgc cacttccggc gcgcaagcct cccggcggcg
     6001 gcggcgccga ggagggagcc cagggtgaag gccacgggaa acttcaggta ttccgtcagc
     6061 ggcaccggat cattcgcgtc cggcgcctgt ccgtcgggca gtccgggcat cggaaagagc
     6121 tcgtgcacga tgaccaccac ggcgaccgcg acgaggccac cgaggaggat tccgagaatc
     6181 atggacgcga aagtccgcat ggaagaagac tcatggaacg gccctgcttc agggtaacgg
     6241 ctgaacggac cggaatcgcg acgggatcgt gcctcatggt acggaaaatt gcggtcgcgg
     6301 gggattcccc cggaggcgaa tcggcagtga cccgggcgcg cccctgctgc tggtcctgca
     6361 ggtccccggg acctggattc ttgcatcatc cgattacggg acagtaattt cggggcacgt
     6421 ggacgcccct gcggggggga tcctgtggcg atgaggtccg cgagctaccc ccataatcca
     6481 tcctgagagg cggaccatgc gacgaactgc cgtcgtcctg tacggcgtgg tgagctacgc
     6541 cgtattcctc gtggcattcc tttacgcgat cgggttcacc ggcaacgtgc tggtgcccaa
     6601 gtccatcgac agcgggcccg cgtacccgct cggagacgcg ctcctcatca acctgctgct
     6661 gctgggcctg ttcgcgatcc agcacaccat catggcccgg cccgtgttca agagctggtt
     6721 cacccggttc gtgccccgcg aggtcgagcg cagcacgttc gtgctcgtgg cgtctctgct
     6781 gcttctgctt ctctactgga agtggcgcgc cgtggacggc atcgtgtggg acgtgtccgg
     6841 cacgatgggc ggaacggtgc tcaccgccct cttctggatc ggctgggcga ccgtgctggt
     6901 ctcgacgttt ctcatcgatc atttcgacct gttcggattg cgccaggtgt acctgcacta
     6961 caccggacag gcgtaccacc atccgccgtt cgtgacccgc ggactgtacc gtttcgtgca
     7021 gcacccgatc atgatcggct tcatcgtcgg attctgggcg acgcccacca tgaccgtggg
     7081 gcacctgctc ttcgcggtgg tcaccacggc ctacatcttc gtgggagtgc tcttcgagga
     7141 gcgcgacctg accaagatgc tcggtgaacc ctacgcggcc taccggcgcc gcgtgccgcg
     7201 cttcgtcccg ttcctgaagg gacctggatc ggtcgcacag gccggcggag ccgagccggc
     7261 gatggctccc ggcccggtcc ggacgcaaga ccactgaggc cgggaacccc ggcgtcgcga
     7321 aaacggcagc ggcggagtgc acccccgtgc actacgccgc tt
//
LOCUS       DBNHQZ010000114         4682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1252104, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000114 DBNHQZ010000000
VERSION     DBNHQZ010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1252104"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1028)
                     /locus_tag="AC16YU_02950"
     CDS             complement(99..1028)
                     /locus_tag="AC16YU_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064208.1"
                     /translation="MLAVETHSLTHRYGRRTALDDVSVAWPENRMVAVLGPNGSGKST
                     LLRILSTALVPESGTVRVAGFDVRTEADAVRTALGTVFQSPALDPELTVTENLEVFAA
                     LHGVSRREAAESIPALLRGLEIDDRALERVGRLSGGLRRRADLARSLVHAPGMLLLDE
                     PTAGLDPSARAAFWSSLERIRSARPLTLLVSTHDFDEAARCDHLVILDRGRIAVEGSP
                     AGLVGALGPEALWVDCENPDAVRRHVEPGDVVGAAGRRVRISGSSLARTLLRLRDAPD
                     AGVRSLELRPPDLSDVYEAATGRRWSGSGEEAA"
     gene            complement(1042..1974)
                     /gene="cyoE"
                     /locus_tag="AC16YU_02955"
     CDS             complement(1042..1974)
                     /gene="cyoE"
                     /locus_tag="AC16YU_02955"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6064209.1"
                     /translation="MNRPGISVASRSLQEPIAFPAAGYSVWRDWVTLTKPEITFLVTI
                     TAAAGFLLGSPDGVAWGRLVATLMGVALASAGGAALNHVYERPLDALMRRTATRPLPG
                     GRIAPMSATWFGLALVTAGLGLVCPLTNPLTGVLAAITVVLYVLVYTPLKRRTTLNTA
                     VGTIPGALPALGGWTAATGNLAGPGWILFAVLVFWQIPHFLALAWMYRGDYARAEFRM
                     LPVVAPGGRSTAIHVVVASALMIIASLLPFVAGIAGPVYAVGAAAAGIAFVRPVAAFA
                     RTLDNARARRLLRATVYYIPLLLALVVVDRLVGL"
     gene            complement(1971..2897)
                     /locus_tag="AC16YU_02960"
     CDS             complement(1971..2897)
                     /locus_tag="AC16YU_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR6064210.1"
                     /translation="MRARFGFTLTTLAAAVGLLAWGAFVTSIDAGLAVPDWPTSFSSY
                     DPFNPWPEWWTFTPVLAEHGHRLAGALVGLLTLVLAIWTWVADARPWLRRLALGALAL
                     VTLQGILGGLRVVWVSIGFAVTHALVAQLFFALLAALALFTSGAWDRIPGTAAMDRPG
                     ARTLLLAGPLATAAVYLQILFGALLRHPGTGIDGTLSLLHIAWAAATLAAVLVHARLV
                     GRILGDVPSGPALSRSLYVLVTVQVSLGFVAWFILLDEQGMLRPSNLQVVVNSLHLVI
                     GAALFTTNALAVLVARRAAWNPSSALPLAPVP"
     gene            3096..3620
                     /locus_tag="AC16YU_02965"
     CDS             3096..3620
                     /locus_tag="AC16YU_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064211.1"
                     /translation="MALGLVASLGSGLAAPALAARAGDTTAIYGHWLADRLADRAESR
                     TDGSMERLAGSRFPTLRAYVVAFVGDLVREQGLAEASRILGEEPAGSEGRLAGLFMRG
                     LRSLSSDALPSAILLSGPDARTIPIERSAAMPPPVFSAPRVSAGPRCVRPPVRPFAPA
                     VLSPASGGAQPLGP"
     gene            3733..>4682
                     /locus_tag="AC16YU_02970"
     CDS             3733..>4682
                     /locus_tag="AC16YU_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR6064212.1"
                     /translation="MQGNGFKVTLTIFFLVLSGYYLYPSVRSVFMNRTLDSLEGDARV
                     AYEQEHHSQIESLNERSLKLGLDLLGGMHVTLEVRVESLIRELASDTDQTFDEVLAAA
                     RERAVTGDVSVIDAFVEEFELRDPDARLSRYFRNETAGITRRSSNEDVASWLRQEASD
                     AVDRAIEIIRDRVDRYGVTEPSIQKQGTRRVIVELPGIDDAERIRRLLRGTARLEFRL
                     MADPADLVRSLQQVIDHYEVAPDTTDAIAEAEADSTTDVADLLAQDADTTSGNALLDV
                     FQPVGQGVLFGRVSERDTVRVSELLSTRAVQDMLPPNVRL"
BASE COUNT          763 a         1621 c         1678 g          620 t
ORIGIN      
        1 gaagccgacg ggctgtccga ccgcgccgac gatgcggccg cggtcgcgga cgaacttgag
       61 cagttcgcga cgccacaggg cgattacggg cctcatcgtc atgcggcctc ctccccggat
      121 ccggaccacc ggcgccccgt ggcggcctcg tacacgtcgg aaagatcggg cggccggagc
      181 tcgagcgatc gcacgcccgc atccggggcg tcccggagcc gcagcagggt tcgggcaagg
      241 gaggatccgc tgatccggac gcgccgtccc gcggccccga ccacgtcccc cggttcgaca
      301 tgtcgccgga cggcgtcggg attctcgcag tccacccaca gggcctccgg accgagcgca
      361 cccacgagtc cggccggcga accctcgacg gcgatacggc cccggtccag gatcaccaga
      421 tggtcgcacc gggccgcttc atcgaagtcg tgggtcgaca ccaggagggt cagcgggcgc
      481 gcagacctga tccgctccag cgaggaccag aatgcggccc gcgccgacgg atccagtcca
      541 gccgtcggct cgtccaggag cagcattccg ggggcgtgta cgaggctccg tgcgagatcc
      601 gccctgcggc gaagtccgcc ggacagccgt ccgacacgct ccagggcacg gtcgtcgatc
      661 tcgagcccgc gcagaagggc gggaatcgac tcggccgcct cgcggcggga taccccgtgc
      721 agcgccgcga acacttccag gttctccgtc accgtgagtt ccggatcgag cgccggagac
      781 tggaaaaccg tcccgagagc cgtgcgcacc gcgtcggcct cggtcctgac gtcgaatccg
      841 gcgacgcgca ccgtacccga ctccggcacg agagcggtcg agaggatccg cagcagcgtc
      901 gacttgccgc tgccgttggg ccccagcacc gcgaccatcc ggttctcggg ccaggcgacg
      961 ctcacgtcgt cgagggccgt ccgccggccg taccggtgcg tcagcgagtg ggtctcgact
     1021 gccagcatca ggcgttgcag gtcagaggcc cacgagacgg tccacgacga cgagggcgag
     1081 gagcagcggg atgtagtaca ccgtcgccct gagcaggcgc cgcgccctgg cgttgtcgag
     1141 ggtgcgggcg aaggcggcca ccggtcgcac gaacgcgatt ccggccgccg cggcgccgac
     1201 ggcgtacacg ggaccggcga ttcccgcgac gaagggaagc agggatgcga tgatcatgag
     1261 tgcggaggcg accacgacgt ggatcgccgt ggagcggcca ccgggcgcga ccaccggcag
     1321 catgcggaat tcggcccgcg cgtagtcccc ccggtacatc cacgcgagcg cgaggaagtg
     1381 cgggatctgc cagaagacga gtaccgcgaa caggatccag ccaggcccag ccaggtttcc
     1441 ggtcgccgcg gtccagccgc caagcgcggg aagcgcgccc gggatggtgc ccactgccgt
     1501 gttcagcgtg gtccgccgct tgaggggcgt gtagacgagc acgtagagta cgaccgtgat
     1561 cgccgccagg acgccggtca ggggattcgt cagcgggcag acgagcccga gccccgcggt
     1621 caccagcgcg agcccgaacc aggtcgcgga catcggggcg atgcgtccgc cggggagagg
     1681 cctcgtggct gtgcggcgca tcagggcgtc cagcggacgc tcgtagacgt ggttgagcgc
     1741 agcgcctccg gcggacgcca gcgctactcc catgagcgtc gcgacgagtc gtccccatgc
     1801 cacgccgtcc ggcgagccca ggaggaaccc cgcggccgcc gtgatggtca ccaggaacgt
     1861 gatttccggc ttggtgaggg tcacccagtc ccgccacacg gaatacccgg ccgccggaaa
     1921 ggcgatcggt tcctgaaggc tgcgggaggc gacggagatt ccgggtcgat tcacggtacg
     1981 ggtgcgagtg ggagggcgga ggatggattc caggccgcgc gccgggcgac gagcacggcg
     2041 agcgcgttgg tcgtgaagag agccgcgccg atgaccaggt gaagcgagtt gacgacgacc
     2101 tgcaggttgg aggggcgaag catgccctgc tcatcgagca ggatgaacca ggccacgaag
     2161 cccaggctca cctgcacggt gaccagtacg tagagggagc gggacagggc gggtccggag
     2221 ggcacgtccc cgaggatacg gccgacgagg cgggcatgca cgaggacggc ggccagcgtg
     2281 gccgctgccc aggcgatgtg gagcagcgag agcgtcccgt cgatgcccgt tcccggatgg
     2341 cgcagcaacg ctccgaacag gatctgaagg tagacggccg ccgtcgccag ggggccggcg
     2401 aggaggagcg tccgggcccc gggccgatcc atggcggccg tccccgggat acggtcccac
     2461 gcgcccgagg tgaagagcgc gagcgcggcg agcagggcga agaagagctg cgcgaccagg
     2521 gcatgcgtca cggcgaaccc gatcgacacc cacacgacgc gcaggcctcc caggatgccc
     2581 tgaagggtga cgagggcgag cgcgccgagc gcaagccggc gcagccacgg gcgggcgtcc
     2641 gcgacccagg tccagatcgc gaggaccagc gtgagaaggc ccaccagcgc gcccgcgagc
     2701 cggtgcccgt gctcggccag cacgggcgtg aaggtccacc attcgggcca ggggttgaag
     2761 ggatcgtacg acgaaaacga cgtgggccag tccggtacgg cgagaccggc gtcgatcgac
     2821 gtcacgaacg caccccaggc gaggaggccc acggcggcgg cgagggtggt gagggtgaag
     2881 ccgaagcggg cgcgcatgtc ccgggagctg ctggaagcca agaacgaaac gaaccctgac
     2941 ggccgtcgcg cagttccacg ccgccccgcc cgaacggacc cgtagccgag ctgcccgtgg
     3001 agacgccggt ttcgtgccct atattccgcg gctgcgcgcc aggcgagctt cctccaacaa
     3061 tgcattcgat cgaccattcc cgattccgcc gtgccgtggc gctcggcctc gtggcgagcc
     3121 tcggttccgg gctcgcggcc ccggcgcttg ccgcccgggc gggcgacacg acggcgattt
     3181 acgggcactg gctggcggac cgcctggccg accgggccga gagccgcacg gacggctcca
     3241 tggagcgact ggcgggatcg cggttcccga ccctgcgggc gtacgtcgtg gccttcgtgg
     3301 gcgacctcgt ccgcgagcag ggcctggccg aagcctcccg gatcctgggg gaggagccgg
     3361 cggggtccga agggcgcctt gcggggctct tcatgcgggg gctgagatcg ctctcgtccg
     3421 atgcccttcc ctcggccata ctgctctcgg gtcccgacgc gcgcacgatc cccatcgagc
     3481 gttcggccgc gatgccgccg cccgtcttct ccgcgccgcg ggtctccgcc gggcccaggt
     3541 gcgttcgccc gcccgtaagg ccgttcgccc cggcggtcct gtcgccggcc tcgggtggcg
     3601 cgcagccgct cggaccgtag cgtcgaagcc ggtcccgtgc cgggcggcgc ccgtcgcact
     3661 ccacctccga taacgatccg cccgtagtgg gcggacacct catctgttta ttgaaccctt
     3721 caggcaaaga ccatgcaggg aaacggcttc aaggtcactc tgaccatctt ctttctcgtg
     3781 ctctccgggt actacctgta cccgtcggtg aggagcgtct tcatgaaccg caccctggac
     3841 tccctcgagg gggatgcccg ggtggcctat gagcaggaac atcattccca gatcgagtcg
     3901 ctgaacgaac ggtcgctgaa gctgggcctg gaccttctgg gcggcatgca cgtcacgctc
     3961 gaagtccggg tcgaatcgct catccgcgaa ctcgcgtcgg acaccgacca gacgttcgac
     4021 gaggtcctcg ccgccgcgcg ggagcgggcg gtcaccgggg acgtttcggt gatcgacgcc
     4081 ttcgtcgagg agttcgaact ccgtgatccg gacgcccgcc tgtcccgtta cttccgcaac
     4141 gagaccgccg gcatcacgcg tcgctcgtcc aacgaggacg tggcctcgtg gctgcgccag
     4201 gaggccagcg atgcggtgga tcgggccatc gagatcatcc gggatcgcgt ggaccgctac
     4261 ggcgtcaccg agccctccat ccagaagcag ggcacccgcc gcgtgatcgt cgagcttccg
     4321 ggcatcgacg acgcggagcg gatccggagg ctgctgcgcg gtaccgcgag actggagttc
     4381 cggctgatgg ccgatcccgc ggatctcgtc cggtcgctgc agcaggtgat cgaccactac
     4441 gaggtggcgc ccgatacgac ggacgccatc gccgaggcgg aggccgactc cactacggat
     4501 gtcgccgacc tcctggcgca ggacgccgac acgacctccg gcaacgcact gctcgacgtc
     4561 ttccagccgg tcggccaggg ggtcctgttc ggccgggtct cggagcgaga caccgtccgc
     4621 gtgtccgagc tgctctctac ccgcgccgtg caggacatgc tgccgccgaa cgtccgcctg
     4681 at
//
LOCUS       DBNHQZ010000115         5029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_437775, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000115 DBNHQZ010000000
VERSION     DBNHQZ010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_437775"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4989)
                     /locus_tag="AC16YU_02975"
     CDS             complement(<1..4989)
                     /locus_tag="AC16YU_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR6064213.1"
                     /translation="MEPPTATIDSRNSSILHRISRGLRRGAAAAAIVVVAVLVLFATS
                     RGSGLPGPGTGTRSSVTATGPEEGSTLEDRVEVLWTADDPIPVTSAFHVTRDGVLMHI
                     AASTESLWEDFSTTPNVTYEYCVSVVDADATETAIGCDYGSRVIARPTGFTASDGLYE
                     DHVRLQWADQSGIEAGYNIYRDAGYALLLDGKDDYVSPSGIVDLAGSFSFEFWAKRNT
                     ANTEDYVINLETFPNGDPVDVDLEVGFLADGRFAFGFGSNMLVSDVPFMDTGEWHHWA
                     IVYDASTLRRFIFRDGQVVKHDAIAAAVQPTGDLQFGRRTALGQTKYRGHFDGALDEI
                     RMWNVALSLDQIRERRTELLTGSEAGLASYWPMDVENGLVAPDVFGTHPGTLVAMTAT
                     SWVPTGVPQFRITGGPDITAYVDLAAVSGYEEYAYEIAAFEDTDGSGSYSPAADFESE
                     HRTDTGWRARLTPPGDVSATDGQFNDRVAISWLDLSEVEQGFRVYRNSTLLGSVAAGV
                     TGFVDVTAMVDVSYEYCVAAFGGGAESLRMCDAGMAGGLPAPVDVAATHGTYDDRVLV
                     TWVDASDTEDGFEVYRFEAPPVVSDTTLVAILGKDAEEYNDLAAVQGVEYHYGVRAFS
                     KVDPLDPAYSDTIWTTVPGMRAIILDPTNLAATQDDFEDRVELEWDNPSTTVMLFRVY
                     RDDVLVQVLDFNTLETTDRGIPSDTDIEYCVTGATVIGAVDPRAVQGEIAMVLDNLDL
                     EDQVAAKTGGLATTVEDRMAAVYDVLAPITAGKTSAAEALFESDPACGTGSRSLLPPT
                     EVDATDNDSENHVQIEWEDNSQAETGYSIFRTENGGSPVLIATLSANRTTYGDYVGTP
                     GVPFTYTVQAFDADGVSTTDSDTGIRTLQPPTQLIATDAVSETEVRMRWKDNSTAENG
                     YRIYRRVVGSGDFVEVGTTATNEPAFTDLPPSDLRGDSLQYRVVAYDAYGESLGPVDW
                     GMTFIQAPGNVNASDAYPTAPIVVVWVDRSGVETGYRFTRRRKGTAVPDIETVLPVNS
                     TTYPDGTAASGITYVYCVQAMLGADTDKWSGPVCDEGSLYSAPGGGGGGVVDPTLTAV
                     RIGDGSLFTNSLDPAGVRFGASVAITGDSAAVIGAPGADGPGANGAAIFYSLDELNQW
                     TLVSTQQPSAGLEYGHAVDITDGYAVVGDPFAGNDALGAFQAFLVGETLTSIPRIAEG
                     PNAGYSVAVVDSFIVVGNDGYSTGVPNGNGRALVCNVEADPGCTSAVALESLLPDSLK
                     KVNASFGFAVDITRDSTSTGDSLYVIVGAPGDLKAYVFECWLGSANGCSDAASWELGQ
                     VVLSPFESKPATLFGAAVSIDEKVAVVSAPGQLGILMLSRTSLQTWDQLQVLTFATAG
                     GFGTSVKVHDGRVIVGSPTAKASTIESAGAVFVYNWNGSSLSEAPVRYDARVAGTPVT
                     GARFGASVDIGDGFYLVGAPMEGKGAAYSIPFGFEPPLEPVTPPEIDLAAPTRVAASD
                     GSTPDRIQVRWTDNADGESGQIVYRSAAGGALEKLDQVAADAIFYDDLAASPGEAYTY
                     CVTAYAFVEGTLQETLPGCDIGWRPPNGAIAGLVATGGGAGTSEVDVCLTPSPNKALL
                     FDGIGGFVEAPVGDELAMASDFTIEAWIRPHDTTGNQYIVARDSAYAVWMSSG"
BASE COUNT          769 a         1709 c         1757 g          794 t
ORIGIN      
        1 accgctcgac atccacacgg cataggcgct gtcccgcgcc acgatgtact ggttgccggt
       61 cgtgtcgtgc ggccggatcc aggcctcgat cgtgaagtcg gaggccatcg ccagctcgtc
      121 gccgaccggg gcctccacga agcccccgat gccgtcgaag agcagggcct tgttcggact
      181 cggcgtaagg cagacgtcca cctccgaggt tccggcgccg ccgcccgttg cgacgagccc
      241 ggcgatggcg ccgttcggag gccgccagcc gatgtcacag ccgggcaggg tctcctgcag
      301 cgtgccctcg acgaaggcgt aggccgtcac gcagtacgtg taggcctcgc ccggcgacgc
      361 cgcgaggtcg tcgtagaaga tggcgtcggc cgccacctgg tccagcttct cgagcgcgcc
      421 tcccgccgcc gaacggtaga cgatctggcc cgattcgccg tcggcgttgt ccgtccagcg
      481 gacctggatg cggtcgggcg tcgagccgtc ggaagccgcg acacgggtcg gggccgccag
      541 gtcgatctcg ggcggcgtga cgggctcgag cggcggctcg aagccgaacg ggatcgagta
      601 cgccgctccc ttgccttcca tgggggcgcc gacgagatag aagccgtcgc cgatgtccac
      661 ggaagccccg aagcgggcgc cggtcaccgg cgtcccggcg acccgggcgt cgtaccggac
      721 cggcgcttcg gagagcgacg atccgttcca gttgtagacg aagacggcgc cggcactctc
      781 gatggtagag gccttggcgg tcggcgagcc cacgatcacg cgcccgtcat gaaccttgac
      841 cgacgtgccg aatcctccgg ccgtcgcgaa ggtcagcacc tgcagctggt cccaggtctg
      901 cagggacgtg cgcgagagca tgaggatgcc gagctggccc ggggccgaga ccacggccac
      961 cttctcgtcg atcgagacgg ccgcgccgaa gagcgtcgcg ggcttcgact cgaaggggct
     1021 gagcacgacc tgccccaact cccatgaggc ggcgtcggaa cacccgttcg cgctgcccag
     1081 ccagcactcg aagacgtagg ccttcaggtc gcccggtgcc ccgacgatga cgtagaggga
     1141 atcgcccgtg gaggtcgagt cgcgcgtgat gtccaccgcg aagccgaacg acgcgttcac
     1201 cttcttcagc gagtccggga gcagcgactc gagggcgacc gcacttgtgc atcccgggtc
     1261 ggcctcgacg ttgcagacca gggcccggcc gttgccgttc ggcacgccgg tcgagtagcc
     1321 gtcgtttccg acgacgatga acgagtcgac gaccgccacc gagtatccgg cgttaggccc
     1381 ttccgcgatt cgggggatcg acgtcagggt ctcgccaacg aggaaggcct ggaaggcgcc
     1441 cagggcgtcg ttcccggcga acggatcgcc gacgaccgcg tagccgtccg tgatgtccac
     1501 ggcgtggccg tactcgaggc cggccgaggg ctgctgcgtg gagacgagcg tccactggtt
     1561 gagctcgtcc aggctgtaga agatcgccgc gccgttggcg cccgggccgt ccgcgccagg
     1621 ggcaccgatc acggccgcgc tgtcgccggt gatggcgacg ctggccccga agcgcacgcc
     1681 cgccgggtcc agactgttgg tgaacaggct cccgtccccg atgcggacgg ccgtgagcgt
     1741 cggatcgacg acgccgccac ccccgccgcc gggggcgctg tagagtgacc cctcgtcaca
     1801 gaccgggccg gaccacttgt ccgtgtcggc accgagcatg gcctggacgc agtagacata
     1861 cgtgatgcca ctcgccgcgg tgccgtccgg gtacgtcgtc gaattgacgg ggaggacggt
     1921 ctcgatgtcc ggcacggccg ttcccttccg cctccgggtg aaccggtagc ccgtctcgac
     1981 cccggatcga tccacccaca cgaccacgat cggtgcggtg ggataggcgt ccgaggcgtt
     2041 cacgttgccc ggcgcctgga tgaacgtcat gccccagtcc accgggccga gcgattcgcc
     2101 gtacgcgtcg taggccacga ccctgtactg gagcgagtca ccccgcagat cggagggggg
     2161 caggtccgtg aaggccggct cgttggtggc cgtcgtgccc acctcgacga agtcacccga
     2221 tccgacgacc ctccggtaga tccggtagcc gttctcggca gtcgagttgt ccttccagcg
     2281 catccgcacc tcggtctcgc tcacggcatc ggtcgcaatg agctgcgtgg gcggctggag
     2341 cgtccggatg cccgtgtcgg agtccgtcgt cgaaacgccg tcggcgtcga aagcctggac
     2401 cgtgtaggtg aacgggacgc ccggggtacc cacgtagtcg ccgtaggtcg tgcggttggc
     2461 cgagagcgtc gcgatcagga cgggcgagcc gccgttctcg gtacggaaga tgctgtatcc
     2521 cgtctcggcc tgggaattgt cctcccactc gatctgcacg tggttctcgc tgtcgttgtc
     2581 cgtcgcgtcg acctcggtcg gagggaggag cgagcggctg cccgtaccgc acgcgggatc
     2641 cgattcgaag agcgcttccg cggccgacgt cttgccggcc gtgatcggcg ccaggacgtc
     2701 gtacaccgcg gccatccggt cttcgacagt cgtcgcgagg ccgcccgtct tcgcggcaac
     2761 ctggtcctcg aggtccaggt tgtcgagcac catcgcgatc tcgccctgga cggcgcgtgg
     2821 atcgaccgcg ccgatgaccg tggcgcccgt gacgcagtac tcgatgtcgg tgtcggacgg
     2881 gatgccccgg tcggtcgtct cgagcgtgtt gaagtccagg acctgcacca ggacgtcgtc
     2941 ccggtacacg cggaatagca tgacggtcgt ggacgggtta tcccactcga gctcgacccg
     3001 gtcctcgaag tcgtcctgtg tggccgcgag gttcgtgggg tcgaggatga tcgcccgcat
     3061 gcccggcacg gtcgtccaga tcgtatccga gtaggccgga tccagcggat ccaccttgct
     3121 gaaggcccgc actccgtagt ggtactcgac gccctgcacg gccgccaggt cgttgtactc
     3181 ctcggcgtcc ttcccgagga tcgccacgag cgtggtgtcg gaaaccacgg ggggggcctc
     3241 aaaccggtag acctcgaagc cgtcctcggt gtccgacgcg tcgacccacg tcacgagaac
     3301 gcggtcgtcg tacgtcccat gcgtggccgc gacgtcgacg ggggcgggga gaccgcccgc
     3361 catgcccgcg tcgcacatcc ggagggactc ggcgccgcct ccgaaggccg cgacgcagta
     3421 ctcgtaggaa acgtccacca tggccgtcac gtccacgaac cccgtgacgc cggccgcgac
     3481 ggagccgagg agcgtcgaat tgcggtagac gcggaagccc tgctcgacct ccgagaggtc
     3541 cagccacgag atggcgaccc ggtcgttgaa ctgcccgtcg gtggcggaga cgtcaccggg
     3601 aggggtgagc cgggcgcgcc agcccgtgtc ggtcctgtgt tcagactcga agtccgcggc
     3661 cggcgaatac gagccgctgc cgtcggtgtc ctcgaaggcc gcgatctcgt acgcgtattc
     3721 ctcgtagccc gagacggccg ccaggtccac gtaggccgtg atgtccgggc cgcccgtgat
     3781 gcggaattgc ggcaccccgg tcggcaccca cgaggtcgcg gtcatcgcga ccagcgtccc
     3841 gggatgcgta ccgaagacgt cgggggcaac gaggccgttc tcgacgtcca tcggccagta
     3901 gctcgcgagg ccggcctcgg agccggtgag gagctccgtc cggcgttccc ggatctggtc
     3961 caggctcagg gccacgttcc acatacggat ctcgtccagc gcgccgtcga aatggccgcg
     4021 gtacttggtc tgcccgaggg cggtccgccg gccgaactgg aggtcgcccg tcggctggac
     4081 ggcggccgcg atggcgtcgt gcttgacgac ctggccgtcg cggaagatga agcgccgcag
     4141 ggtcgacgcg tcgtagacga tggcccagtg gtgccattct cccgtgtcca tgaacggcac
     4201 gtcggacacc agcatgttgc tcccgaaccc gaaggcgaac cggccgtcgg ccaggaagcc
     4261 cacctcgagg tccacgtcca ccgggtcgcc gttcggaaag gtctccaggt tgatgacgta
     4321 gtcctccgtg ttcgccgtgt tgcgcttcgc ccagaactcg aacgagaagc tgcccgcgag
     4381 gtcgacgatg ccggacgggg agacgtaatc gtccttgccg tcgaggagga gggcgtagcc
     4441 cgcgtcgcgg tagatgttgt agcccgcctc gatcccggac tggtccgccc actggagccg
     4501 gacgtggtcc tcgtacaggc cgtcgctcgc cgtgaatccg gtgggccggg cgattacacg
     4561 ggagccgtag tcacagccga tcgcggtctc cgtcgcatcg gcgtcgacga cgctgacgca
     4621 gtactcgtac gtgacgttcg gagtcgtgga gaagtcctcc cagagcgact cggtggacgc
     4681 tgcgatgtgc attaggactc cgtcgcgcgt cacgtggaac gcgctcgtga ccgggatcgg
     4741 gtcgtcggcc gtccagagca cctcgacccg gtcttccagc gtgctcccct cctcgggccc
     4801 ggtcgccgtg accgagctgc gcgttcccgt gcccgggcct ggcaggcccg atccccggga
     4861 cgtggcaaag aggaccagca cggcgaccac gacgatcgct gccgcggcgg caccccggcg
     4921 caggccgcgg gagatgcggt gaagaatgga cgagttgcgt gagtcaatcg tagccgtggg
     4981 cggttccatg acagtctcct ggagttatcg agtgagcggc gtgcccgga
//
LOCUS       DBNHQZ010000116         4353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1658258, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000116 DBNHQZ010000000
VERSION     DBNHQZ010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1658258"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3463
                     /gene="metH"
                     /locus_tag="AC16YU_02980"
     CDS             <1..3463
                     /gene="metH"
                     /locus_tag="AC16YU_02980"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR6064214.1"
                     /translation="TFTSTSVAQEDYGLGSLALEMNREAARIARKAADARTAATPDRP
                     RWVAGALGPTNRSLSISPDVTDPGRRSITFARLSAAYREQVAGLVEGGADILLVETVF
                     DTLNAKAAIHAIRSYFRETGQRRPVMISGTIVDQSGRTLSGQTPEAFWISVAHAPDLL
                     SVGLNCALGSAQMRPFLETLSGVVTVRTSLYPNAGLPNAFGEYDESPEFMASQVDEYA
                     GDGYLNLVGGCCGTTPEHIRAIVDAAARHEPRVPPARTGLLALAGLEPLVFRDDLNFV
                     NIGERTNVTGSRRFARLIREGNYEEALSIARQQVESGAQMIDVNMDEGMLNSEEAMVR
                     FLNLVAVEPDIARVPVVIDSSRWSVIEAGLQCVQGKPVVNSISLKEGEAAFREQAERA
                     LGYGAAVIVMAFDENGQADTLERRRAVCRRAYSILVDEVGMAPEDVIFDPNIFAVATG
                     IEGHNRYAVDYIETVRWIRQNLPHARTSGGVSNVSFSFRGNERVREAMHSAFLYHAIH
                     AGMDMGIVNAGQIGVYEAIDPELLEAVEDVLLDRRSDATERLVTLAERFAGTSSAGSA
                     AEAEWRSLPVDGRLRYAMLQGIVDHIEEDTEEARQKATAAIQVIEGPLMEAMGHVGDL
                     FADGQMVLPQVVKSARVMKKAVAYLVPFIEQEKSDRDTPRSRHRILLATVRGDVHDIG
                     KNIVGVVMGCNDYEIIDLGVMVPADRIVEEARRLEVHAVGLSGLITPSLDEMVHVARE
                     MDRAGYTIPLLIGGATTSRIHTAVRIAPAYRGPVVHVLDASRSVGVASRLLDPDARDE
                     FAAAVREEYETLRTRHGKGPTSTGLLSLGEARENAFTSDWSAVPVTRPASPGVRVLDD
                     VPVAVLRRYIDWTPFFQAWELRGHYPKLLDDPERGEEARTLLADAEELLDRIERDGVL
                     RPKGVLGVFGAASRGDDILVFDGDGRDRPRAVLHTLRQQSRKSAGRPNRALADFVAPV
                     DSGVEDWIGAFAVTAGHGLDGLVREFEEEHDDYGAIMAKALADRLAEAFAEYLHELVR
                     REAWGYAQDETLANDDLIAERYRGIRPAPGYPAQPDHTEKRVIWDLLGVTEAAGIELT
                     ESLAMWPAASVCGLYFAHPDAAYFNVGVLGRDQVADYAGRKGMPLDEMERWLSPRLAY
                     DPAAAGD"
     gene            3534..4112
                     /locus_tag="AC16YU_02985"
     CDS             3534..4112
                     /locus_tag="AC16YU_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR6064215.1"
                     /translation="MNSIGLQADRPAAIDALRSRDASVSVQAAGIIGFALLTAVGAQF
                     RLYLWEVPFTLQTLAVYGSGLYLGWRNGLLAQLLYLALGFFVPVFAGEGQGIAYLAGA
                     ASAGYLLAAPLSAAAAGALSRRWRGLTGSIVSMAVASLILFTAGVVWLHFAAGHATWL
                     ESIDRGWLRFLPVDAAKILLLGLVYAGSRRVR"
BASE COUNT          633 a         1519 c         1550 g          651 t
ORIGIN      
        1 cacgttcacg tcgacctcgg tcgcccagga ggactacggg ctcggctcgc tcgccctgga
       61 gatgaaccgc gaggcggcgc gcatcgcccg gaaggccgcg gacgcccgaa cggcggcgac
      121 ccccgaccgg ccccggtggg tcgctggcgc actcgggccg acgaaccgat cgctgtcgat
      181 ctcgcccgac gtcaccgatc ccggccgacg gtccatcacg ttcgcgcggc tgtccgccgc
      241 gtaccgcgag caggtggccg gcctcgtcga gggcggagcg gacattctcc tcgtcgagac
      301 ggtcttcgac acgctcaacg ccaaggccgc gatccacgcc atccgctcgt acttccgcga
      361 gacggggcag cggcggccgg tcatgatctc gggtacgatc gtggaccaga gcgggcggac
      421 gctctccggc cagacgcccg aagccttctg gatctccgtc gcgcacgccc cggacctcct
      481 gtccgtgggc ctgaactgtg cgctgggatc cgcccagatg cggccgttcc tcgaaaccct
      541 gtccggcgtc gtgacggtga gaacgagtct ctatccgaac gcgggcctgc cgaacgcctt
      601 cggggaatac gacgagtcgc ccgagttcat ggcatcccag gtcgacgagt acgcaggaga
      661 cggctacctg aacctggtcg gcggatgctg cggcacgacg cccgagcaca tccgcgccat
      721 cgtcgacgcc gctgcccggc acgaaccccg cgtgccgccc gcccggaccg gacttctcgc
      781 acttgccggg ctcgagccgc tggtgttccg cgacgacctg aacttcgtga acatcgggga
      841 gcggacgaac gtgacgggct cgcgacgctt cgcccggctc atccgggagg ggaactacga
      901 ggaagccctg tcgatcgccc gccagcaggt ggaaagcggc gcccagatga tcgacgtgaa
      961 catggacgag gggatgctga actcggagga ggccatggtg cgcttcctga acctcgtcgc
     1021 ggtcgagccg gacatcgcca gggtgcccgt tgtgatcgac tcgtcgcgct ggtccgtgat
     1081 cgaggccggg ctccagtgcg tccagggaaa gccggtggtg aactcgatca gcctgaagga
     1141 gggcgaggcg gcgttccgcg agcaggcgga gcgcgccctg gggtacggcg ctgcggtgat
     1201 cgtgatggcc ttcgacgaga acggccaggc cgacacgctc gagcggcgca gggcggtctg
     1261 ccgccgggcg tactcgattc tcgtggacga ggtcgggatg gccccggagg acgtcatctt
     1321 cgatccgaac atcttcgccg tggccacggg catcgagggc cacaaccggt acgcggtcga
     1381 ctacatcgag accgtgcgct ggatcaggca gaacctgccg cacgcccgga cgtcgggcgg
     1441 cgtgagcaac gtctcgttct ctttccgggg gaacgagcgc gtgcgcgagg cgatgcacag
     1501 cgccttcctg taccatgcca tccacgccgg catggacatg ggcatcgtga acgcgggcca
     1561 gatcggcgtc tacgaggcga tcgaccccga gctgctcgag gccgtcgagg acgtgctcct
     1621 ggaccgccgg tccgacgcca ccgagcgcct cgtaaccctc gccgagcgct tcgccggcac
     1681 ctcgtcggca ggctcggccg ccgaggccga gtggcggtcg ctgccggtcg acgggcggct
     1741 gcgctacgcc atgctccagg gcatcgtgga tcacatcgag gaggacacgg aggaggcgag
     1801 acagaaggcc accgcggcga tccaggtcat cgagggcccg ctcatggaag cgatgggcca
     1861 cgtgggcgac cttttcgcgg acgggcagat ggtccttccg caggtggtca agagcgcgcg
     1921 cgtgatgaag aaggccgtgg cctacctggt gccgttcatc gagcaggaga aatcggaccg
     1981 ggacacgccc cggtcgcggc accggatcct gctcgccacc gtgcggggcg acgtgcacga
     2041 catcggcaag aacatcgtcg gcgtcgtgat gggctgcaac gactacgaga tcatcgatct
     2101 gggcgtcatg gtgccggcag accgcatcgt cgaggaagcc cggcgcctcg aggtgcacgc
     2161 cgtggggctc tccgggctga tcacgccgtc gctcgacgaa atggtgcacg tggcgcgcga
     2221 aatggaccgg gcggggtaca cgattccgct cctcatcggc ggggcgacga cctccaggat
     2281 ccacaccgcc gtgcgcatcg cgcccgcgta ccgggggccg gtggtccacg tgctcgacgc
     2341 gtcccgaagc gtgggcgtgg cctcgcggct cctggatccg gacgcccgcg acgagttcgc
     2401 ggcggccgtc agggaggagt acgagacgct ccgcacccgg cacggcaagg gcccgacctc
     2461 gaccggcctc ctgagcctcg gggaggcccg cgagaacgcc ttcacgagcg actggtcggc
     2521 cgtccccgtc acccgtccgg ccagtcccgg ggtgcgcgtc ctggacgatg tccccgtcgc
     2581 ggtgcttcgc cggtatatcg actggacccc gttcttccag gcctgggagc tgcgcgggca
     2641 ctacccgaag ctgctcgacg atcccgagcg cggggaggag gcccgcacgc tcctcgccga
     2701 cgcggaggaa ctgctcgacc gcatcgagcg cgacggcgtg ctccggccga agggcgtgct
     2761 cggggtgttc ggcgcggcgt cccggggcga cgacatcctg gtgttcgacg gggacggccg
     2821 cgaccggcca cgggcggtcc tgcacacgct ccgccagcag tcgcgcaagt ccgcgggccg
     2881 tccgaaccgg gccctcgccg atttcgtcgc ccccgtggat tcgggagtcg aggactggat
     2941 cggcgccttc gcggtcaccg cgggtcacgg tctcgacggc ctggtgcggg agttcgagga
     3001 ggagcacgac gactacgggg cgatcatggc gaaggcgctc gccgaccgac tggccgaagc
     3061 gttcgccgaa tacctgcacg agctcgtccg ccgcgaggcc tggggctacg cccaggacga
     3121 gacgctcgcg aacgacgacc tcatcgcgga gcgataccgg ggcatccgtc ccgcaccggg
     3181 gtacccggcc cagccggacc acaccgagaa gcgagtgatc tgggacctgc tcggcgtgac
     3241 ggaggcggcg ggcatcgagc tcacggaatc cctggccatg tggcccgcgg cgtcggtgtg
     3301 cggcctgtac ttcgcccatc cggacgcggc gtacttcaat gtcggcgtcc tgggtcgcga
     3361 ccaggtcgcg gactacgccg ggcgcaaggg gatgccgctc gacgagatgg agcggtggct
     3421 ctcgccccgg ctcgcctacg atccggcagc cgcgggggac tgatccggcc gggtggcgcc
     3481 gcagggcgcg tggcggctat cttgcgcgac gctccgacac gcacccacga ttcatgaatt
     3541 ccatcggtct gcaggccgac cgcccggcgg ccatcgacgc cctgcgctcc cgcgacgcat
     3601 ccgtttccgt ccaggcggcg gggatcatcg gcttcgcgct gctcacagcg gtcggcgccc
     3661 agttccgcct ctacctctgg gaagtgccct tcacgctcca gacgctcgcc gtctacggga
     3721 gtggcctgta cctgggctgg cggaacgggc tcctcgccca gctcctgtac ctcgcgctgg
     3781 gcttcttcgt gcccgtgttc gccggggagg gccagggcat cgcctacctg gccggggccg
     3841 cttcggccgg ctacctgctc gcggcaccgc tgtcggccgc cgcggccggt gcgctgtcgc
     3901 gccgctggcg gggtctcacc gggagcatcg tatcgatggc cgtggcctcg ctcatcctgt
     3961 tcacggccgg tgtcgtctgg cttcacttcg cggccggaca cgccacctgg ctggagtcga
     4021 tcgaccgggg ctggctccgg ttcctgccgg tggacgcggc caagatcctg ctgctgggcc
     4081 tcgtgtacgc cgggtcgcgg cgcgtccggt gattcggggc gcgctgtccg atcgcgagta
     4141 cagcccacgt ccttttcggc gccggagggg ccggttcggg aaccggcacg gcattggcac
     4201 ggtcgacggt gacgcccatt ctccaggccg gtccatgaag cgaacgacgg tcttcctcct
     4261 cgtcctggcc ctcgcgccgg ctgccctggc ccaggtaccc acgcggtacg gctggcacgg
     4321 gccgtatgga tcggtgctgg acgatcccga gac
//
LOCUS       DBNHQZ010000117         4192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_710641, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000117 DBNHQZ010000000
VERSION     DBNHQZ010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_710641"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="AC16YU_02990"
     CDS             complement(<1..1847)
                     /locus_tag="AC16YU_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6064216.1"
                     /translation="MRRISHIAPWLLVLAGLALPAAGQGSLEGRITDADLGEPLIGVN
                     VRVVGTSFGAATDLEGRFQIANLRAGDYSIQLSYIGFETRLYTGIAIRNGETTILDAE
                     LHQAVLSADEEVVIIGERPLVDVEESSSTSRISRDQLSAAPLRDVQAAVASQVGIVQD
                     PTGLYIRGGRAEETGFIVDGVSAKDPLAGTGFGLDLGSNAFSEIEVTTGGVGAEYGDV
                     TSGVVSVQTQDGTDRLAGTFSHKRDDLGINDDAESNFREELYELTLSGPLVRGKLRFF
                     VSGQVQLSDGFTRLVSTPDQVRSSLVGSTFFMPRTGNRWNGLTKLTWELRPGTRLQGS
                     YQRSLTVNQNTRMLQVTGNDAIVQPGFQYAFILQPDNANTYAHDNVITYLKWSQVLGE
                     SSFFDFQVSRLFTRLRADANGRRWRPTAVDSELDPFSVVTWPPEVFVDQSGQPLDVNA
                     LFVLPGPGLVNNGGIATRFHDHFAEEWTARGTYTRFSRSKAHRTTAGFELKFNDYQWI
                     DVERPWVGAPIGTGEGTSSNRLGESSDIWRVKPRRGGLFATHQVRFRGLIANAGLRLE
                     YWSPGAYVDRLVDDPLAPIPESVRASYRSGTVNLFGLRTKIRMLPKLRVS"
     gene            complement(1844..3445)
                     /locus_tag="AC16YU_02995"
     CDS             complement(1844..3445)
                     /locus_tag="AC16YU_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064217.1"
                     /translation="MRCRLAHILMPVLLAASCDAGFDGLPGENRPPDTALSVRDENLM
                     DNLGEEGRLRSTIRLSWTGDDPDGFVAGFDVRVHPFGEDPAAETGWTHTTANDSLLLL
                     PLPGGESTANVVVEVRAVDELGLRDPSPARSVFPIRNSPPEIRLGQFELPPDTTFTVV
                     SFAWTARDPDGEQNLASIGISLNDSTRFVEIPPEFDFVTLVAEQDQVPSGGEVEARLY
                     LGRGVQATAIRVPGLRLDAENTFYVRARDQADTTSTVERHTWWVKANRSDVLFVNDYR
                     LASNGTLLGYHSSVLSDHLPAGYGFDLWDITRPYTTGSSGTVPRSDALPPVAEPMLRM
                     TLSLYRYIYWVSTSTTGDPGTDNMPFVAPAMPEFFANGGKLLVHSPVTVSSVESDFSD
                     NAAVLLLPLSRPLILPDSIGRLELRPNALVTPTATAQAMGLPEIASLRFFINVRPYEA
                     VGAAVEPLYSGAFIYRTTTGGSGTWTEPTTVASISADRRIALFSLPLVNDLTLEPQFR
                     LAEGTGEAGPMIIHALLDALSFPAR"
     gene            complement(3494..>4192)
                     /locus_tag="AC16YU_03000"
     CDS             complement(3494..>4192)
                     /locus_tag="AC16YU_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064218.1"
                     /translation="RFGMTQLRDVVFTVQSAGNPLHEPTILESTSALADRNIAESLEG
                     MFVRVEDVTVFATNADAPSGPFGEFLVTSDGTGENAVRVNDQSTAISYTGNDPGTVFS
                     EGQLLDFVQGTLWFSFSNFKIEPGTFADIQVHSGVATEDETVPTTSRLHQNFPNPFNP
                     TTAIRYDVATTGRVTLAVYDALGRMVAMLENGERAVGTYTSSLDASHLASGLYVYRLT
                     TADRTVSRTMTLVK"
BASE COUNT          673 a         1378 c         1494 g          647 t
ORIGIN      
        1 gagacccgca gcttgggcag catccggatc ttggtcctca ggccgaacag gttcaccgtg
       61 ccgctgcggt acgaggcgcg gacgctctcc ggaatcggcg ccaggggatc gtcgacgaga
      121 cggtccacgt acgcacccgg cgaccagtac tcgagccgga ggccggcatt cgcgatgagg
      181 ccgcggaagc ggacctggtg ggtggcgaaa aggcccccgc ggcgcggctt cacccgccag
      241 atgtccgagc tctcgccgag ccggttggag ctcgtgccct cgcccgttcc gatgggagcc
      301 cctacccatg gccgctcgac gtcgatccac tggtagtcgt tgaacttcag ctcgaagccg
      361 gcggtggtcc ggtgcgcctt cgatctcgag aaccgcgtgt acgtgccgcg ggcggtccat
      421 tcctcggcga agtggtcgtg gaagcgcgtg gcgatgccgc cgttgttcac gaggccaggt
      481 cccggcagca cgaagagcgc attgacgtcg agtggctgcc cggactggtc cacgaacacc
      541 tcgggcggcc aggtcacgac gctgaacggg tcgagctcgc tgtcgaccgc cgtgggtctc
      601 cagcgccgcc cgttcgcatc ggcccggagg cgcgtgaaga gccgcgaaac ctggaagtcg
      661 aagaacgagc tctcgccgag gacctgcgac cacttcagat acgtgatgac gttgtcgtgc
      721 gcgtacgtat tggcgttgtc gggctgcagg atgaaggcgt actggaaacc cggctgcacg
      781 atcgcgtcgt tgcccgtcac ctgcagcatg cgcgtgttct ggttcaccgt cagcgaacgc
      841 tggtacgaac cctgcaggcg cgtgcccggg cgcagctccc aggtgagctt cgtgaggccg
      901 ttccagcgat tacccgtccg cggcatgaag aaggtggatc cgacgagcga cgatcggacc
      961 tggtcgggcg tggagaccag gcgcgtgaac ccgtcggaga gctgcacctg gcccgagacg
     1021 aagaagcgca gcttcccccg caccaggggg ccgctgagcg tgagctcgta gagttcctcg
     1081 cggaagttgg actccgcgtc gtcgttgatt ccgagatcgt cccgcttgtg ggagaacgtg
     1141 ccggcgagcc ggtcggtgcc gtcctgggtc tggacggaga cgacgcccga agtcacgtcc
     1201 ccgtactcgg ccccgacgcc gccggtggtc acctcgatct cggagaaggc gttcgatccg
     1261 aggtcgagcc cgaagccggt gcccgcgagg gggtccttgg cggagacgcc gtccacgatg
     1321 aagccggttt cctcggcccg accgccgcgg atgtacaggc ccgtcggatc ctgcacgatg
     1381 cccacctggg acgccaccgc cgcctgcaca tcgcgcagcg gcgcggccga gagctggtcc
     1441 cgcgagatgc gtgacgtgga ggaagattcc tccacgtcca cgagcgggcg ctccccgatg
     1501 atcacgacct cctcgtcggc cgagaggacc gcctggtgca gctccgcgtc gaggatcgtc
     1561 gtctcgccgt tccggatcgc gatgccggtg tagagacgcg tctcgaaacc gatgtacgag
     1621 agctggatcg aatagtcgcc cgcgcgcagg ttggcgatct ggaagcggcc ctccaggtcc
     1681 gtggccgcgc cgaacgaggt gcccacgacg cggacgttca cgccgatgag gggctcgccg
     1741 aggtcggcat ccgtgattcg cccctcgaga ctgccctgcc cggcagcggg aagggccagg
     1801 ccggccagga ccaggagcca gggcgcgata tgggaaatcc gcctcatcgc gcggggaacg
     1861 agagcgcgtc gagcagcgcg tggatgatca tggggcctgc ctcgcccgtg ccctccgcca
     1921 ggcggaactg cggctcgagc gtcaggtcgt tgacgagcgg gagcgagaac agggctatgc
     1981 gccggtccgc gctgatggaa gcgaccgtcg tgggctccgt ccacgtgccc gacccacccg
     2041 tcgtggtcct gtagatgaag gcgcccgagt acagcggctc cacggccgcg ccgacagcct
     2101 cgtacgggcg gacgtttatg aagaagcgca gcgacgcgat ctccggcagg cccatcgcct
     2161 gcgccgtggc cgtcggggtc acgagcgcgt ttggccggag ctcgagcctt ccgatcgagt
     2221 cggggaggat cagcggccgg gagaggggca gcagcagcac cgcggcgttg tccgagaagt
     2281 ccgactcgac ggaagacacc gtgacggggg agtggaccag gagcttgcct ccgttcgcga
     2341 agaactccgg catggccggc gccacgaagg gcatgttgtc cgtcccggga tccccggtgg
     2401 tgctcgtcga cacccagtag atgtaccggt agagcgaaag cgtcatgcgc agcatgggct
     2461 cggccaccgg cggcagcgca tccgaccgtg gcaccgtgcc agaggagccg gtcgtgtacg
     2521 ggcgcgtgat gtcccagagg tcgaagccgt agccggcagg caggtggtcc gagagcacgg
     2581 acgaatgata gccgagcagc gtgccgttcg aggccagccg gtaatcgttc acgaacagca
     2641 cgtcgctgcg gttggccttg acccaccacg tatgccgctc gacggtgctc gtcgtgtcgg
     2701 cctggtcccg cgcgcggacg tagaacgtgt tctcggcatc gagcctgagt cccggcaccc
     2761 ggatcgcggt cgcctgcacg ccccgcccga ggtacaggcg ggcctccact tcgccgccgg
     2821 acgggacctg gtcctgttcg gcgaccaggg tgacgaaatc gaactcgggc gggatctcga
     2881 cgaaccgtgt cgagtcgttg aggctgatgc cgatcgaggc caggttctgt tcgccgtcgg
     2941 gatcccgggc ggtccaggcg aacgagacca ccgtgaacgt cgtgtcgggc ggaagctcga
     3001 actggcccag ccggatctcc ggcggcgagt tgcggatcgg gaagacgctc cgggccggag
     3061 acggatcgcg cagcccgagc tcgtccacgg cccggacctc gaccacgacg ttggccgtgg
     3121 actctccgcc cgggagcggc agcagcagaa gggaatcgtt cgccgtcgtg tgggtccacc
     3181 ccgtctccgc cgccggatcc tcgccgaacg ggtgcacccg cacgtcgaat cccgccacga
     3241 acccgtccgg atcgtccccc gtccacgaca ggcggatggt cgagcgcagc cggccctcct
     3301 cgcccaggtt gtccatcagg ttctcgtcgc gcacgctcag ggccgtgtcg ggcggacggt
     3361 tctctcccgg caggccatcg aagcccgcgt cgcaggaggc cgccaggagg accggcatca
     3421 ggatatgcgc gagacggcag cgcacggaag ggacgggcgg ggcccggcac ggggccccgc
     3481 ccgcggttcc ggtttacttc accagcgtca tcgtgcggga gaccgtgcgg tccgccgtcg
     3541 tgagacggta cacgtacagg ccgctggcca ggtgcgaggc gtcaaggctg gacgtgtagg
     3601 tccccaccgc gcgctccccg ttctccagca tcgcgaccat gcgtccgagc gcgtcgtaca
     3661 cggcgagcgt cacgcggccg gtcgtagcga catcgtagcg gatcgcggtg gtcgggttga
     3721 agggattcgg gaagttctgg tgcaggcggc tcgtcgtcgg gacggtctcg tcctccgtgg
     3781 cgacaccgct gtggacctgg atgtcggcga aggtgccggg ctcgatcttg aaattgctga
     3841 aggagaacca cagcgtgccc tgcacgaagt cgagcagctg gccctcgctg aacaccgtgc
     3901 cgggatcgtt gccggtgtac gagatggccg tcgactggtc gttcacgcgg acggcgttct
     3961 cgcccgtgcc gtcggacgtg accaggaact cgccgaaggg accgctcggt gcgtccgcgt
     4021 tcgtcgcgaa gaccgtgacg tcctcgaccc gcacgaacat gccctcgagt gattcggcga
     4081 tgttccggtc cgcaagggcg ctcgtcgact ccaggatggt cggctcgtgg agcggattgc
     4141 cggcgctctg gaccgtgaag acgacgtcgc gaagctgtgt cataccgaag cg
//
LOCUS       DBNHQZ010000118         4844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2335982, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000118 DBNHQZ010000000
VERSION     DBNHQZ010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2335982"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3106
                     /locus_tag="AC16YU_03005"
     CDS             <1..3106
                     /locus_tag="AC16YU_03005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064219.1"
                     /translation="VFVANSDGILVESGGAWSLVPTPARTAVRSISYGHGGLIYAGAI
                     GELGYLGTTRHGELKFVSLAEHLPPDEQEFGDVWTTHAATNGVYFQTSYRVYFWDGAN
                     FTVYRSEGGYHTSFLIDDELIVRERGRGLVRLKNGQTELLPGGDFYAEKRVHVLMKET
                     TGFLVGTRDNGFFRWNGAKSERVVTEADPILKRAWLYGSAELHTGEIALATLGAGVVV
                     IDRQGHLTAHLNTANGAPDDNITSLYRLRSGLVVATTHNAGLFTFYSPSLLTEVAAFE
                     FGAINDILLDSEYAFISTGGGGLTLSMDSVRAAVRGQRRPVGWWNGLKTSWTAVRAGG
                     YVYAATEEGLFRARADRIVADRAPSFTPVNSEFQVFALGTSGADTVLAGYRRGLGVAK
                     ASEWLHQQAGTDIDELVSIVADASGIWATTRIDGVRLYGRPDPFGSPPVKYSVSDGLP
                     ANEKLVVVMAGGAPLYFTKEAPGFYRFDASSGTFRKDHSLAGSGIENAAVFDVHEASD
                     GTIWLALSDGIARATPSRSGGFVVERPELLQFVRSRNTILATESGGALWFTLDDRILR
                     YEPSMEAPVDMEYNAMIERIVSTATDSLLFGGFQFGTTSRGLSRFDLELPHERRGIRV
                     EYGAPMLFDIGRPVEYRTLLEGFDKEWQPWSLSTSRTISEEEEGEYLLRIEARNMYGI
                     VSREARVYLTILPPWYRSWWAYAFYVIGSVFVVTFTVKYRRMVKAQKRAQEQAEELAR
                     ERRYSQRLQEANDQLKRANRLKDEFLATTSHELRTPITAILGFTEVLHNEIPADAPYR
                     EFVSIIEESGQRLMGTLSSLLDVARIRAGTMELVPQPVDLRETVRQSAETRRGQAEGK
                     GLYFKVVLPEEPIDALQDPSAIERVIQHLLDNAVKFTEQGGITVEVAREGEHASIRVH
                     DTGLGIDAAFLPSLFEEFVQESDGERRSHNGNGLGLAIVGGLVRIMGGRVDVHSMKGV
                     GSVFKVLVPIEGAAGEPGSVVGAESGWEYPGSEIQRSGAGDRHPFGPTRLRKVTVPRE
                     ARAD"
     gene            3183..4844
                     /locus_tag="AC16YU_03010"
     CDS             3183..4844
                     /locus_tag="AC16YU_03010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6064220.1"
                     /translation="MRAIPAVFAVLSIAACVEVGPEVIYTNGVVWTGPAGSETAEALA
                     VSGDRITAIGTTGEIRDLARAATREIDLQGRFVVPGFIDNHVHFLSGGFQLASVDLRD
                     AATPEEFAQRIRQHAERVPPGTWITGGDWDHELWGGELPDREWIDAVTPDHPVFVTRL
                     DGHMGLANSLALQTAEVAEDAPDPEGGTVVRTASGRLTGILKDEAMSLVYRVVPDPSP
                     EAFDSALLRAMEHAAALGVTQVHDVGSYGGWQDLEVFRRAHARGALTLRIYSLVPLAS
                     WETMRDEVSAVGRGDEWLRWGGLKGFVDGSLGSTTAWFYEAYDDEPATAGLLVSDTSA
                     VRRHILAADAAGLHLAIHAIGDRANDWLLDVYEDAIAVNGDRDRRFRIEHAQHLAPSS
                     IGRFASLGVIPSMQPYHAIDDGRWAEKRIGPARIQTTYAFRSLVDQGATLTFGSDWTV
                     GPLDPLEGIYAAVTRRTIDGANPDGWVPQQKISVEEALHAYTAANAFAGFQEHDAGTL
                     APGKLADFVVLSDDLRSVEPTSIPQVRVLRTVVGGRTVYTAEPAE"
BASE COUNT          844 a         1491 c         1653 g          856 t
ORIGIN      
        1 ggtcttcgtc gcaaattcgg acggtatact tgtcgaatca ggtggggcgt ggagtctcgt
       61 acccacgcca gcgcgtacgg ctgttcgctc catatcctat gggcatggtg ggctcatata
      121 tgcgggagcg ataggcgaac tcggctacct cggcaccact cgccacggag agctcaagtt
      181 cgtctcgctc gctgaacacc tgccgcccga cgagcaggaa ttcggcgatg tgtggacgac
      241 gcatgccgcg acgaacggcg tctacttcca gacgagttac cgggtgtact tctgggacgg
      301 cgccaatttc accgtctacc ggtcggaggg cggatatcac acctcgttcc tgatcgacga
      361 cgaactcatc gtccgtgagc gggggcgtgg ccttgtccgg ctgaagaacg gccaaaccga
      421 gctcctgccg ggcggggact tctacgccga aaagcgagtg cacgtcctga tgaaagagac
      481 caccgggttc ctcgtgggca cgcgggacaa tggcttcttc cgatggaatg gcgcaaaatc
      541 cgagcgggtg gtgaccgaag ccgaccccat cctcaagcgg gcctggctct acgggagcgc
      601 cgagttgcat accggcgaga ttgcgcttgc gacgctcgga gcgggggtag tggtcatcga
      661 tcgtcaggga caccttacgg cccatctgaa tacggcgaac ggggcaccgg acgacaacat
      721 cacttcgctg taccggttgc gctccgggct ggtggttgcg acgacgcaca acgccggtct
      781 tttcaccttt tattctccca gcctgctgac agaggtggcc gcattcgagt tcggcgccat
      841 caacgacatc cttctcgact ccgaatatgc cttcatctcc accggcggcg gtggactgac
      901 cctgagcatg gactccgttc gggcggccgt gcgcgggcag cgcaggccgg tcggctggtg
      961 gaacggcctg aagacttcgt ggacggcggt tcgggccggc gggtacgttt atgcggccac
     1021 ggaggagggg ctgtttcggg cccgtgccga tcgcattgtg gccgatcgag ctccgtcctt
     1081 cactccagtg aattccgagt tccaggtctt cgcgctgggc acctccggtg ccgacacggt
     1141 actggccgga taccgacgtg gccttggcgt ggcgaaagcc tcggagtggc tccaccagca
     1201 ggcaggcacg gacatcgacg aactggtgtc cattgtggcg gatgcgtccg gtatctgggc
     1261 tacgacgcgc atcgacgggg tgcgtctgta cggtcgaccg gacccattcg ggagtccccc
     1321 ggtcaagtac tccgtgtcgg acgggctacc ggcgaacgag aagctcgttg tcgtcatggc
     1381 gggcggggcg ccgctctatt tcacgaagga ggcgcctgga ttctatcgtt tcgacgcaag
     1441 ctccgggacc ttccggaagg accattcgct ggccgggagc ggtatcgaga acgcggcagt
     1501 attcgacgtg cacgaggcaa gcgacggtac gatctggctc gcgctgagcg acggcatcgc
     1561 ccgggccaca ccgtcccgct ccgggggatt cgtggtcgag cgcccggaac tcctccagtt
     1621 cgtgcggagc cgcaacacga tactcgccac ggaatccggt ggggcgctgt ggttcacgct
     1681 tgacgaccgg atcctccgct acgagccgtc gatggaggcg cccgtggaca tggaatacaa
     1741 tgccatgatc gagcgcatcg tgagcacggc gacggattcg ctcctgttcg gtggattcca
     1801 gttcggtacc acgagccggg gcctctcacg ctttgacctg gagcttccac acgagcggcg
     1861 gggcattcgt gtggaatacg gcgcgccgat gctgtttgat atcggccggc cggtggagta
     1921 tcgcaccctt ctggagggtt tcgataagga gtggcagccc tggagcctct cgacctcccg
     1981 cacaatctcc gaagaagagg agggagaata cttgctccgc atcgaagccc ggaacatgta
     2041 tggaatcgtg tcccgcgagg cgcgggtgta cctgacgatt ctgccaccct ggtaccgatc
     2101 ctggtgggcg tacgcgttct atgtcatcgg cagtgtcttc gtcgtgacct tcacggtgaa
     2161 gtaccgccgg atggtgaagg cgcagaagcg ggcacaggag caggcggaag agctcgccag
     2221 ggagcgccgg tacagccagc ggctccagga ggccaatgat cagctgaaac gggcgaaccg
     2281 tctgaaggac gagtttctgg ccacgacgtc gcacgagctg cgcactccca ttaccgcgat
     2341 cctgggcttc accgaggtgc tacataacga gattccagcc gatgcgccgt accgggaatt
     2401 cgtatccatc atcgaggaga gcggtcagcg cctaatgggc acgctgtcgt cccttctgga
     2461 tgtcgcgcga atccgggcag gcacgatgga gcttgtgccg cagccggtag acctccgtga
     2521 gaccgtgagg caaagcgcgg aaacgcggcg aggccaggcc gagggaaagg gactttactt
     2581 caaggtcgtg ctgcccgaag agccgataga cgcactgcag gatccgagtg ccatcgagcg
     2641 ggtgatccag cacctgctcg acaacgcggt caagttcacg gaacagggcg gcatcacggt
     2701 agaggttgcc agggaagggg agcacgcctc gatccgcgta cacgataccg gattgggcat
     2761 agatgctgcg ttccttccaa gcctgttcga ggagttcgta caggagtcgg acggcgagcg
     2821 ccgttctcac aacgggaacg gcctcgggct ggcgatcgtc ggagggctcg tccgcatcat
     2881 gggtggaagg gtggatgtcc actcgatgaa gggtgtcggc tccgtcttca aggtcctcgt
     2941 ccccatcgaa ggcgcggcgg gcgagccggg ctccgtcgtc ggtgcggagt cgggatggga
     3001 atacccgggg tcggagattc agcgatcggg ggccggggac cgtcacccgt tcggcccgac
     3061 aaggctgcgg aaggtgaccg tgccacgcga ggctcgcgcc gactgacgcc gtacacgagg
     3121 cgcggagaac gtcggatccg tactattgac gggtccgtca ctggccatcc agttcccgcc
     3181 ccatgcgtgc catcccagcg gtgtttgccg tgctttctat cgccgcctgc gtcgaggtcg
     3241 gccccgaagt catctacacg aacggtgtcg tatggaccgg tccggcgggg agcgagaccg
     3301 ctgaggcgtt ggccgtgagc ggggacagga ttaccgcgat aggtacgacg ggcgaaatcc
     3361 gtgaccttgc ccgcgcggcg acccgggaga ttgacctgca gggccgattc gtcgtcccgg
     3421 gattcatcga caaccacgtc catttccttt cgggcggctt ccagctcgcg tcggtcgacc
     3481 tgcgtgatgc cgcgactccc gaagagttcg cgcagcgaat caggcagcac gccgagcgcg
     3541 tgccacccgg gacctggatc accggtggcg actgggacca cgaactctgg ggcggcgagc
     3601 tgcccgaccg cgagtggatc gatgccgtca cgcccgatca cccggttttc gtcacgcggc
     3661 tcgacgggca catgggcctc gcgaactccc ttgccctcca gaccgcggaa gtcgcggagg
     3721 atgccccgga tccggagggt gggaccgttg tccggacggc atcgggacgc ctgacgggta
     3781 ttctcaagga cgaggcgatg tccctggtgt atcgcgtcgt tcccgacccg tcgccggagg
     3841 cgttcgattc cgcgctcctc cgcgccatgg agcacgcggc cgcgctcggg gttacgcagg
     3901 tccacgatgt cgggtcttac ggtggatggc aggacctcga ggtcttccgc agggcgcacg
     3961 cccgcggtgc acttactcta cgcatctatt cgctcgtccc actggccagc tgggagacga
     4021 tgagagacga ggtctccgcc gtcgggaggg gtgacgaatg gctgcgctgg ggtggcctga
     4081 agggcttcgt cgacggatcg ctcgggtcca cgacggcctg gttctacgag gcgtacgacg
     4141 acgaaccggc cacggcgggt ctcctggtca gcgacacgtc tgccgtccgg aggcacatcc
     4201 tggccgcgga cgccgcgggc ctgcatctcg cgatccacgc gatcggggac cgggccaacg
     4261 actggctgct cgacgtctac gaggatgcca tcgccgtgaa cggcgaccgg gaccggagat
     4321 tccgcatcga gcacgcgcag caccttgcac cgtcctctat cggcaggttc gcgagtctcg
     4381 gcgtgattcc gtccatgcag ccctaccatg ccatcgacga cggacgatgg gcggagaagc
     4441 gcatcgggcc cgcgagaatc cagacgacct atgccttccg atccctcgtg gaccagggtg
     4501 caacgctgac cttcggctcg gactggacgg tcggcccgct cgatccgctc gagggcattt
     4561 acgcggcggt cacgcggcgg acgatcgacg gggcgaaccc cgacggttgg gtcccgcagc
     4621 agaagatctc cgtcgaggaa gcgctgcacg cctacacggc ggcgaatgcg ttcgcgggtt
     4681 tccaggagca cgatgccggc acgttggccc ccgggaagct ggccgacttc gtggtcctgt
     4741 ccgacgacct caggtcggtg gagccgacct cgattccgca ggtgagagtg cttcggacgg
     4801 tggttggcgg aaggacggtc tatacggcgg agccagcgga gtga
//
LOCUS       DBNHQZ010000119         4575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2064670, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000119 DBNHQZ010000000
VERSION     DBNHQZ010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2064670"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1011)
                     /locus_tag="AC16YU_03015"
     CDS             complement(31..1011)
                     /locus_tag="AC16YU_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6064221.1"
                     /translation="MIDLVTGASGFIGSALAKRLVAEGRTVRALVRASSRHDLIGPAG
                     DRLRLHAGDVTDPVSLAEAFEGVGRVFHCAGRIGYGGRREARRLFAVNVVGTANILDA
                     AERGGARRVVHVSSVAAIGRPARPERPVDENAEWTGSRLDSAYGRTKYLAELEAQRAV
                     AEGMDVVIVNPSLVFGPGRPGETTMRIVELVRRGRARAYPAGGTNVVDVEDVVTGILA
                     AMDRGQTGRRYILGSENLTWKEILGTLADAFGRPRPRFRIPPAALAAVGALSELASPF
                     GTPRGGLGRESARIASGSVRFDASRARTELAWSPRPFAETAARLASSHPG"
     gene            complement(1008..1526)
                     /locus_tag="AC16YU_03020"
     CDS             complement(1008..1526)
                     /locus_tag="AC16YU_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6064222.1"
                     /translation="MPRESYHVETGHRTLRFEWDGQTLYRDGRPVPATLRQVGEGRYS
                     VLVDGRSLSLSLEGRSNGTLRLRTRDGLRSLTVRDRRALVLRELGMENGRRSGEAALL
                     APMPGLIVQVLVSPGDRVGAGDGLAVLEAMKMENELRAVEDAVIGAVHVQAGTAVGKG
                     QVLIEFGDRHAP"
     gene            1672..2520
                     /locus_tag="AC16YU_03025"
     CDS             1672..2520
                     /locus_tag="AC16YU_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HIR6064223.1"
                     /translation="MMRWMTLLPLLLAVPAGQSSAQPSEPFERVMDSAREHTWQSLPL
                     GEIVQRVGEQFLGAPYEGGLLDRSAGESLHTGLDRFDCVLFVESALALARGIAEGDFD
                     HDSFARRIEEQRYRDGRRDGYCSRLHYFTEWIRDNERRGTVRDITREIGGEALPRRLD
                     FMGRHRDSYPQLADDSLYAGILDMEERLEDHVFHYVPESRIRSVYPLLEAGDIVAFVT
                     RVEGLDVSHTGLVYDAGDGRRGVLHASLDRGVIISPDLQAFVEGNRSQTGIVIARPVD
                     HRDDAK"
     gene            2517..3515
                     /gene="pfkA"
                     /locus_tag="AC16YU_03030"
     CDS             2517..3515
                     /gene="pfkA"
                     /locus_tag="AC16YU_03030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6064224.1"
                     /translation="MSPSAVQAIRRVGVFTSGGDSPGMNACVRSVVRSALAYDLEVMG
                     IRRGYQGMIEGDVVEMDSQSVSNILQKGGTILKSARSAEFRTPEGRARAAETLRAAGI
                     DGLIAIGGDGTLRGAATFYDEYRVPLVGCPGTIDNDLFGTDETIGYDTALNTAIENID
                     RIRDTADAHDRLFLIEVMGRDSGFIALNSGIGGGAELVLIPEQLTDMNEVKRQILSQM
                     TGQTRSSIVVVAEGDELGGATSVASALREDPAFEHVDLRVCILGHTQRGGSPTARDRV
                     LASRLGAAAVDALMEGHANVMVGVVEGEITLTPAKNVWSRKKSIDYDLIKLTNLLR"
     gene            3512..4255
                     /gene="ubiE"
                     /locus_tag="AC16YU_03035"
     CDS             3512..4255
                     /gene="ubiE"
                     /locus_tag="AC16YU_03035"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR6064225.1"
                     /translation="MTDEIRAGSHFPPAGESDGKAVQVAAMFDAIAPRYDLLNRVLSL
                     GIDQRWRREAVRRLGRHEPRRVLDVATGTGDLAIEALSVGAEAVVGVDIAERMLEVGR
                     RKIAAMGLTGRVSLQYGDALRLPFGDGEFDAALVAFGVRNFENLEAGLAEIRRVLRPS
                     GLLVVLEFSRPRRFPIRQLYGFYSRFILPAIGRLVSGDSGAYRYLPASISVFPDGEDF
                     LAAMRRAGYGAVSDRRLTFGVASIYTGEA"
     gene            complement(4289..>4575)
                     /locus_tag="AC16YU_03040"
     CDS             complement(4289..>4575)
                     /locus_tag="AC16YU_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6064226.1"
                     /translation="AYKGEGEKPIDVAVIIQPDRLTVRIRDVGESFDPAKYREPDLVQ
                     FARNRKTGGFGVHIMRQLMDQVEYRTRGGTNECCMTKYRDDLPDTAHPGP"
BASE COUNT          676 a         1546 c         1668 g          685 t
ORIGIN      
        1 gtacggcgcg tccggtgagg aacggcatgg tcatccgggg tgggacgagg cgaggcgcgc
       61 cgccgtctcg gcgaagggtc gaggggacca cgcgagctcc gtgcgggccc tcgaagcatc
      121 gaaccggacg cttcccgagg cgatccgggc ggattccctg ccgagcccgc cccggggagt
      181 cccgaacggg gaggcgagct ccgagagggc accgacggcg gcgagggcgg cgggagggat
      241 gcggaaccgg ggacgtggcc ggccgaaggc gtccgccagg gtgccgagga tttccttcca
      301 ggtgaggttc tcgcttccga ggatgtaccg acgccctgtc tgcccccggt ccatggcggc
      361 caggatgccc gttacgacgt cctcgacgtc cacgacattc gtgccgccgg ccgggtaggc
      421 ccgggcgcgt ccgcggcgga cgagttcgac gatccgcatc gtggtctcgc cgggccggcc
      481 gggcccgaag acgagggatg gattgacgat caccacgtcc atgccctcgg ccaccgcgcg
      541 ctgcgcctcg agctcggcca ggtacttggt ccggccgtac gcggagtcca gccgggaacc
      601 ggtccattcg gcattctcgt cgaccggccg ctccgggcgg gctggtctcc cgatggcggc
      661 gaccgaggag acgtggacca cgcgacgcgc accgccgcgt tccgcggcgt cgaggatgtt
      721 ggcggtcccc acgacgttga cggcgaatag gcgccgggcc tcgcgccggc ccccgtaccc
      781 tatgcgccct gcgcagtgga acacgcgccc gacgccttcg aacgcctcgg cgagtgacac
      841 ggggtccgtg acgtccccgg cgtggagccg gagccggtcc cccgccggac cgatcaggtc
      901 gtgcctggaa gaggcgcgaa cgagggcgcg aacggtcctg ccctccgcca cgagtcgctt
      961 cgcgagcgca gatccgatga aaccggatgc accggtcact aggtcgatca cggcgcgtgc
     1021 ctgtccccga actcgatgag cacctgtccc ttcccgacgg ccgtgccggc ctgcacgtgc
     1081 acggcgccga tgacggcgtc ctcgaccgcc cgcagttcgt tctccatctt catggcctcc
     1141 aggacggcga ggccgtcgcc cgccccgacc cggtcaccgg gagagacgag gacctggacg
     1201 atgagtccgg gcatcggggc caggagcgcc gcctcgccgg atcggcgtcc gttctccatg
     1261 cctagctccc gcaggaccag cgcccggcgg tccctgacgg tgagcgagcg caggccgtcc
     1321 cgggtccgga gccggagcgt tccgttcgag cgaccttcga gcgagaggct caggctccgg
     1381 ccgtcgacga gcaccgaata gcgaccctcg ccgacctggc gtagcgtggc cggcaccggc
     1441 cggccgtccc ggtacagggt ctggccgtcc cactcgaacc ggagggttcg atggcctgtc
     1501 tccacgtgat aggattcccg gggcatgggg gtgttcgacg gatcgcacgc cgtgcggcgc
     1561 cccggcgaat atacgccctc cacccgccaa gtgccggagc ggggcccggg cgggcgggac
     1621 atgcaaccgg cgcgggcgga tgcgagtacg acggctgcac caggcagaaa gatgatgcga
     1681 tggatgaccc tgctgccgct cctcctggcc gttccggccg ggcagtcgag cgcgcagcct
     1741 tcggagcctt tcgagcgggt aatggattcg gctcgcgagc acacgtggca gtccctgccc
     1801 ctcggcgaga tcgtccagcg cgtgggggag cagttcctgg gagccccgta cgagggcggg
     1861 ctcctggacc gttcggccgg ggagtcgctc cataccgggc tggaccggtt cgactgcgtg
     1921 ctcttcgtgg agagcgcgct cgcgctcgcc cgcggaatcg ccgagggcga cttcgaccac
     1981 gactcgttcg cgcgccggat cgaggagcag cgctaccgcg acggcaggcg ggacgggtac
     2041 tgcagccgcc tgcattactt caccgagtgg atccgggaca acgagcgcag gggcacggtt
     2101 cgtgacatca ctcgggagat cggaggagag gcgctcccca ggcgcctgga cttcatgggc
     2161 cgtcatcgcg acagctatcc gcagctcgct gacgacagcc tttacgcggg catcctcgac
     2221 atggaggagc gtctcgagga tcacgtgttc cactacgtgc ccgagtcgcg catccggtcg
     2281 gtctaccctc ttctcgaggc gggcgacatc gtagccttcg tcacgcgcgt agagggactc
     2341 gacgtctcgc acaccgggct cgtgtacgac gcgggggacg ggagacgggg cgtgctgcac
     2401 gcgtcgctcg accggggggt gatcatctcg ccggacctcc aggcgttcgt cgagggcaac
     2461 cggtcgcaga cgggcatcgt gatcgcgcgg ccggtggacc accgcgacga cgcgaaatga
     2521 gcccctctgc cgtccaggcc atccgccgcg tcggggtatt cacgagcggc ggcgactcgc
     2581 cgggcatgaa cgcctgcgtg agatcggtcg tgcggtcggc cctggcctac gacctcgagg
     2641 tcatgggcat ccgccgcgga taccagggca tgatcgaggg cgacgtcgtg gagatggact
     2701 cccagtcggt ctcgaacatc cttcagaagg gcggcacgat cctgaagagc gcccggtccg
     2761 cggagttccg cacgcccgag ggccgggcga gagcggcgga gacgctccgg gcggcgggca
     2821 tcgacgggtt gatcgccatc ggcggtgacg gaaccctccg aggtgcggcc accttctacg
     2881 acgaataccg ggttccgctc gtcgggtgcc ccggaacgat cgacaacgat ctcttcggca
     2941 ccgacgagac gatcgggtac gacaccgccc tgaacacggc catcgagaac atcgaccgga
     3001 tccgggacac ggcggacgcg cacgaccggc tgtttctcat cgaggtcatg ggccgcgatt
     3061 cgggattcat cgccctcaac agtggcatcg ggggcggggc cgagctcgtc ctgatccccg
     3121 agcagctgac cgacatgaac gaggtcaagc gccagatcct gtcgcagatg acgggccaga
     3181 cccgctcgtc gatcgtcgtc gtagcggagg gggacgagct cggcggcgcc acgagcgtgg
     3241 cgagcgccct gcgggaggac ccggcgttcg agcacgtcga cctgcgcgtg tgcatcctcg
     3301 gccatacgca gcgtggcggc tcgcccacgg cccgcgaccg ggtgctcgct tcgcggctcg
     3361 gcgccgccgc cgtggacgcg ctcatggagg gacatgcgaa cgtcatggtg ggcgtggtcg
     3421 agggcgagat cacgctcacc cccgcgaaga acgtctggag ccggaagaag agcatcgatt
     3481 acgacctgat caagctgacc aacctgctcc gatgacggac gagatccggg ccgggagcca
     3541 cttcccgccg gccggcgagt ccgacggcaa ggcggtgcag gtggcggcca tgttcgacgc
     3601 gatcgcgcca cggtatgacc tgctgaaccg cgtgctgagc ctgggaatcg accagcgatg
     3661 gcggcgggag gccgtccgtc ggctgggccg tcacgagccc cgtcgcgtgc tcgatgtcgc
     3721 gacggggacg ggtgacctcg cgatcgaggc cctttccgtg ggcgcggagg ccgtcgtcgg
     3781 cgtggacatt gccgagcgca tgctcgaagt cgggcggcgg aagatcgcgg cgatggggct
     3841 gacgggccgc gtctccctgc agtacggcga cgcactgcgg ctgccgttcg gggacgggga
     3901 gttcgacgcg gccctcgtgg cgttcggcgt gcgcaatttc gagaacctgg aggccggact
     3961 cgccgagatc cggcgcgtgc tgcgtccatc ggggctgctg gtggtgctcg agttcagccg
     4021 gccgcggcgc tttcccatcc ggcagctgta cggcttctac agccggttca tcctgccggc
     4081 gatcggtcgc ctggtatccg gcgattccgg ggcataccgg tacctgccgg cctcgatcag
     4141 cgtgtttccg gacggggagg acttcctggc cgcgatgcgc cgggcggggt acggcgccgt
     4201 gtccgaccgg aggctgacgt tcggcgtggc atcgatctac acgggcgagg cctgacggtc
     4261 cggccaccgg ggccgggccg gcggaaggct acgggcccgg atgcgccgtg tcgggcaggt
     4321 cgtcgcggta cttggtcatg cagcactcgt tggtgccgcc ccgggtgcgg tactcgacct
     4381 ggtccatgag ctgccgcatg atgtggacac cgaacccccc ggtcttccgg tttctcgcga
     4441 actggaccag gtcgggctcc cggtacttcg ccgggtcgaa cgattccccg acgtcccgga
     4501 tgcggacggt gagccggtcc ggctggatga tgaccgcgac gtcgatgggc ttctcgccct
     4561 cacccttgta tgcgt
//
LOCUS       DBNHQZ010000120         3550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_710782, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000120 DBNHQZ010000000
VERSION     DBNHQZ010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_710782"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC16YU_03045"
     CDS             <1..339
                     /locus_tag="AC16YU_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064227.1"
                     /translation="FEVGDGLFVLRGPGAGAPTDLFLAQWEAGPRLLTWVTDLAFDHC
                     GEDGCARLESWLVDLDGDGRRDVVRRSRREDAETGAVEDVLEAWLLAGDGTLRATTLP
                     AGMRARFRAD"
     gene            478..729
                     /locus_tag="AC16YU_03050"
     CDS             478..729
                     /locus_tag="AC16YU_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064228.1"
                     /translation="MKCPLNARVYVAGAALGIALFVVAWDGFFLLLRELGYVDGGASS
                     TLIAVLFLTCMLVMCVGYGVGIAMFVSRKLRRPSGPRAA"
     gene            complement(1134..3206)
                     /locus_tag="AC16YU_03055"
     CDS             complement(1134..3206)
                     /locus_tag="AC16YU_03055"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR6064229.1"
                     /translation="MIQGSLAPFFAPGHVVFIGASRTPGRLGWVLAHNLVEHGFRGEV
                     SFVNPKGGSLFDRPLHPSLDDVPGTPDLAVLLIPAALIPDALDQCGRRGIRAAIVASG
                     GFSETGDAGRELERRIREVLDRHGMRLVGPNCIGIMDSDSGLDTAFLSGPGLLAGPVA
                     FVSQSGAVCAAVIEWARGDGFGLSRLFSLGNQVDVTETDVLEAIGEDTNSTVVTLYLE
                     GVDDGRRFVDVASEVTRRKPVIALKVGRSEAGRRAAASHTGALAGRDVAYEAAFRRAG
                     ILRARSSEDVFDWARVLAQGPFGPAHRLAILTNAGGPGVAATDAAAVEGLDVVPLTDT
                     TRARLRDFVPPEGAFGNPVDMLAAASPETYGGALRVLLDADEVDSVLVILPPPPGWPA
                     EDVADAIIAEARGSTKAVVVALMGDAGIRVARERLREAGVPAFRYPERAVRALGAVEA
                     AEKARLRPGGSARAATVGPLPAPGPDGWLDAEDAAGVVARSGIDVPPMIVAPDADSAA
                     AAQAALGAPVAVKLRSRTIVHKTDVGGVLLGVDSPGAAADAFVAIREGAGAAAFGGVL
                     VQPMAGPGTDLIVGAVRDEQFGPLVMVGTGGTDVETEADAAFDLAPLTRVQAADLLDR
                     SGAARRLVARRGQPAADREAVIDAIVRIAALAAGAPSIREMDINPLRVSTAGAVALDV
                     RIRVDEAG"
     gene            complement(3203..>3550)
                     /locus_tag="AC16YU_03060"
     CDS             complement(3203..>3550)
                     /locus_tag="AC16YU_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6064230.1"
                     /translation="CNRMLGFVNAPALMQRVIAEAVDAMPDIAHYEAKKDLLCAALRR
                     AGYRFAEPEGTFYIFPETPGDEADFVARAKERLLLVVPGSAFGRPGHFRISFAAPDST
                     VRLACRLLEELNA"
BASE COUNT          463 a         1308 c         1324 g          455 t
ORIGIN      
        1 ttcgaggtcg gggacgggct gttcgtgctg cgcgggccgg gcgccggggc gccgaccgac
       61 ctgttcctcg cccagtggga ggcgggcccg aggcttctta cgtgggtcac ggacctcgcc
      121 ttcgaccact gcggggagga cggctgcgcg cggctcgaga gctggctcgt ggacctggac
      181 ggcgacggcc ggcgcgacgt ggtgcgccgc tcgagacggg aggacgcgga gacgggcgcg
      241 gtcgaggacg tgctggaggc gtggctcctg gcaggggacg gcacgcttcg cgcgaccacg
      301 cttccggccg ggatgcgggc gcgcttccgg gcggactgaa ccgcgcggtc ccgggggaga
      361 gcgcgtcgcc acgtccgcga agcgggccac gaggcgcgcc tcggccgcgg gaccgtcgtc
      421 acgaaggatt ctcatgccgc gctggcccgg cgcccgtcgc cgggcgaact tgtcggcatg
      481 aaatgcccgc tgaatgcccg cgtatacgtc gccggcgccg ccctgggaat cgcgctcttc
      541 gtggtggcct gggacggctt cttcttgctg ctgcgcgagc tcgggtacgt ggatggcggg
      601 gcgtcctcga cgctcatcgc cgtcctcttc ctcacgtgca tgctcgtgat gtgcgtgggc
      661 tacggcgtgg gcatcgccat gttcgtgtcg cggaagcttc gccgcccgtc cggcccgcgg
      721 gcggcctgag cagggccgcg gggatcacgg gggcgcaggc cgcgcagggc cgcccgtcgg
      781 cgcggcgagc cgggcgcgcg aacgggaggg acgcaaccac cggtcgcagg ctccgtatcg
      841 ggagtcgaaa cgcaaccgcg atccgggcca tgaacctcct gtccatcctt tccgccgtgc
      901 cgctcgtctc gctgctccgg gtgcgccagg agctgctggg acgcgcgatc gcccgcgtcg
      961 gctgacctcg atgagcttct ggcgtcagtt caagcgtgcc atcaaggcgc gactcagggg
     1021 gctgttttcc tggaagaagc cctactgacg ggccgcgtcc ccggcgcccg gcgttttccc
     1081 ggcgcctgcg cttcttcccc gcgcccggcg gcccgcccgc ctccggaatc cagtcagccc
     1141 gcctcgtcca cccggatccg cacgtccagg gcgaccgcgc ccgcggtcga cacgcgcagc
     1201 ggattgatgt ccatctcccg gatcgacggc gcccccgcgg ccagcgccgc gatgcgaacg
     1261 atcgcgtcga tgaccgcctc ccggtcggcg gcgggctgac cgcggcgggc cacgaggcgc
     1321 cgggcggcgc ccgagcggtc cagcaggtcg gccgcctgaa cgcgcgtcag gggcgcgagg
     1381 tcgaaggccg cgtcggcttc cgtctccacg tcggtacctc ccgtcccgac catgaccagc
     1441 ggaccgaact gctcgtcgcg cacggccccc acgatgagat ccgtgccggg acccgccatc
     1501 ggctgcacca gcaccccgcc gaacgccgcc gcgccggcgc cctcgcggat cgcgacgaat
     1561 gcgtcggccg cggcgcccgg cgagtcgacc cccagcagga cgcccccgac gtccgtcttg
     1621 tggacgatgg tccgggagcg gagcttgacg gccaccggcg cgccgagggc ggcctgcgcc
     1681 gcggcggccg agtcggcgtc cggggccacg atcatgggag gaacgtcgat gccggagcgc
     1741 gccacgacgc ccgccgcgtc ctccgcgtcg agccagccgt ccggtccggg cgcgggcagg
     1801 gggccgacgg tggcggccct cgccgatccg ccgggccgca ggcgcgcttt ctcggcggcc
     1861 tcgacggccc cgagggcccg gaccgcccgc tccggatagc ggaacgcggg cacgccggcc
     1921 tcgcgcagcc tttcccgggc cacgcggatc ccggcatcac ccatcagggc gacgaccacg
     1981 gccttcgtcg agccgcgggc ctcggcaatg atcgcgtcgg ccacgtcctc ggccggccag
     2041 ccggggggag ggggcaggat gaccagtacc gagtccacct cgtccgcgtc gagcaggacg
     2101 cggagcgcgc cgccgtaggt ctcgggcgag gcggcggcca gcatgtccac cgggttgccg
     2161 aaggcgccct cgggcggcac gaagtcgcgg agacgcgccc gcgtggtgtc ggtgagcggc
     2221 acgacgtcga gcccttccac ggccgccgcg tcggtggccg cgacgccggg gccgccggcg
     2281 ttcgtgagga tggccaggcg gtgcgccggc ccgaacgggc cctgggcgag gacccgcgcc
     2341 cagtcgaaga cgtcctcgct gctgcgtgcc ctcaggatgc ccgcccgcct gaacgccgcc
     2401 tcgtaggcca cgtcgcgccc ggcgagggcc ccggtgtggg acgcggcggc ccggcgcccg
     2461 gcctcgctgc gcccgacctt gagcgcgatc acgggcttcc tccgggtgac ctccgaggcg
     2521 acgtccacga agcggcggcc gtcgtccacg ccctccaggt acagcgtcac gaccgtcgag
     2581 ttcgtgtcct cgccgatcgc ctccaggacg tcggtctcgg tgacgtcgac ctggttgccg
     2641 aggctgaaga ggcgtgacag tccgaacccg tcgccgcggg cccactcgat caccgccgcg
     2701 cagacggcgc ccgactgcga gacgaaggcc accgggccgg ccagcaggcc gggacccgag
     2761 aggaaggccg tgtccagccc cgaatcggag tccatgatcc cgatgcagtt ggggccgacg
     2821 aggcgcatgc cgtgtcggtc cagcacctcg cggatgcggc gctcgagctc acgcccggcg
     2881 tctcccgtct cggaaaagcc gccggacgcg acgatcgccg cccggatgcc ccggcgtccg
     2941 cactggtcca gcgcgtcggg gatgagcgcc gccgggataa ggagcaccgc gaggtccggc
     3001 gtccccggca cgtcgtccag ggacgggtgg agcgggcggt cgaagagtga gccgcctttc
     3061 gggttcacga acgagacctc tccccggaag ccgtgttcga ccaggttgtg cgccagcacc
     3121 cagcccagcc ggcccggcgt gcgcgaggcc ccgatgaaca cgacgtggcc gggtgcgaag
     3181 aagggggcga gcgatccctg gatcatgcgt tgagctcctc gagcaggcga caggccaggc
     3241 ggacggtcga gtccggcgcg gcgaaggaaa tgcggaaatg cccgggccga ccgaacgccg
     3301 atcccggcac cacgagcagc aggcgctcct tggcgcgcgc cacgaaatcg gcctcgtcgc
     3361 ccggcgtctc ggggaagatg tagaaggtgc cctcgggctc ggcgaaccgg taacctgcgc
     3421 gccggagcgc cgcgcacagg aggtccttct tggcctcgta gtgcgcgatg tcgggcatcg
     3481 cgtccacggc ctccgcgatc acccgctgca tgagcgcggg cgcgttcacg aagcccagca
     3541 tgcggttgca
//
LOCUS       DBNHQZ010000121         5048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2608064, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000121 DBNHQZ010000000
VERSION     DBNHQZ010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2608064"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..889
                     /locus_tag="AC16YU_03065"
     CDS             44..889
                     /locus_tag="AC16YU_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064231.1"
                     /translation="MTIRIPTSALAAALTLVAVPACDTVVDPFDRDVDRRFAVHGYLD
                     AAADTQFVRVQALRRAADGGDDTLAGVVVRSDDGPAMAWQGETGTDSEGEPVHVFTAV
                     FRPMPGERYGIVLERDGVQGARAVTVVPPVPGVVAAPGEGTAISFTKDITLTSLGVLD
                     AEMVVWYEVVNPKTGLPEREEMAYGLPGPSAGPDRTFTTFLSQDRAEILRRLGLGPEF
                     RGVELLRTGAHVEILSAEWDSAAPDPGLEDGHGFFASVGRYDVTWLLPTAEVTTMGMV
                     DRQQR"
     gene            1030..1956
                     /locus_tag="AC16YU_03070"
     CDS             1030..1956
                     /locus_tag="AC16YU_03070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6064232.1"
                     /translation="MPNPDIWFNGKLVPHESATVHVLSHALHYGTSVFEGIRSYKTER
                     GSAIFRLREHMQRLVDSARIYRMEIPYSLEELEKAVTDTIEGSGLEACYIRPLVFRGH
                     GPMGVNPLKNPVETVVAVWEWGAYLGAEALEQGVKVEVASWQRMAPNTFPSLAKAAGN
                     YLNAGLVKMDAVLNGVVEGIMLSTNGFVAEGSGENLFLVKGGVLYTAPAALSILPGIT
                     RDSVMRIAADLGYEVKESLIPREALYLADEVFFTGTAAEVTPVREIDHHVIGSGRMGP
                     VTARIQEAFFDVIRAGNDPYGWLTPVNVGAVA"
     gene            complement(1967..2431)
                     /locus_tag="AC16YU_03075"
     CDS             complement(1967..2431)
                     /locus_tag="AC16YU_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6064233.1"
                     /translation="MKLQDLIRQDHFDSAGHEAILNVIVTGSWVAGEMSAAMAPFGVT
                     PAQYNVLRILAGAYPNRVTCSEIGGRLLDRTPDVTRLLNRLDKAHLISRSRSRTDRRV
                     VRVGINEAGLELLQRMAPVVTELQVRIARHVTEEDLRRLSELLEKLRTDQRT"
     gene            complement(2514..3536)
                     /locus_tag="AC16YU_03080"
     CDS             complement(2514..3536)
                     /locus_tag="AC16YU_03080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064234.1"
                     /translation="MPHRIAAKVALVATALVGVPLVALSWATLGPAPVHLVAIPVVFA
                     GAYLAAGVVLGRRITLARTVLREIRRSRFENLETARLPRGDELNDLVWQVYRTGQVLE
                     REMQDLRKTETYRKEFLGNVSHELKTPIFAIRGFTETLLGGAVDDGRVNRSFLDKILR
                     NSDRLANLVRDLGEISRIESGELTMIMAPFDLRAMVCEVVESLEPIARDRSVTLRTTV
                     PVGLPRAVGDADRLRQVLVNLVDNAIKYNEPDGQVEVVARHVAGGRIKLSIVDDGIGI
                     DPDHIPRLTERFYRVDRSRSRSQGGTGLGLAIVKHILAAHAAHLVVESRPGRGSTFGF
                     SLRAAD"
     gene            complement(3658..4347)
                     /locus_tag="AC16YU_03085"
     CDS             complement(3658..4347)
                     /locus_tag="AC16YU_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6064235.1"
                     /translation="MAPSPSKVLVVDDEEDIVELLRYNLEHAGLDVVEARDGAEAITV
                     ARRENPDLIVLDIMMPRMDGIEACRRIREHPALKKTPVLMLTARSEEEDHVRGLDVGA
                     DIYLTKPISIPVFLSQVKAMLRGTERFEEIPDVVHAHGLEIDRLRYVVEREGSDSPIR
                     MPRKEFELLRFLAANAGRVFSRQELLDEVWGRDVYVVDRTVDVHVRKIREKIGDSYIE
                     TVKGVGYRFRE"
     gene            4498..>5048
                     /locus_tag="AC16YU_03090"
     CDS             4498..>5048
                     /locus_tag="AC16YU_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6064236.1"
                     /translation="MALGRKLRYGMVGGGPGAFIGGVHRMAAALDGGTELVAGAFSAD
                     PAKSAATGRDLYLDPRRVYGSWAEMADREAARDDGIDFVSIVTPNHLHHAVARAFIER
                     GIHVVCDKPMTNTLDEAEDLCRLVADSGVVFALTHNYTGYPMVKHARHMVGAGELGTV
                     RKIVVEYPQGWLARSTEGTGNKQA"
BASE COUNT          756 a         1673 c         1815 g          804 t
ORIGIN      
        1 agcttccgct cgtgccgtcg gtcggcctga acgtcgaggt ccattgacga tccgcatccc
       61 gacaagcgcc cttgccgcgg ccctgaccct cgtcgccgtg cccgcctgcg acacggtggt
      121 cgatcccttc gaccgcgacg tggatcgccg gttcgccgtg cacggctacc tggacgccgc
      181 cgccgatacc cagttcgtgc gggtccaggc gctgcgcagg gccgccgacg gcggggacga
      241 cacgctggcc ggcgtcgtcg tgcggtccga cgacggtccg gcgatggcgt ggcaggggga
      301 aacgggcacg gattcggagg gcgagcccgt gcacgtgttc acggcggtct tccggccgat
      361 gccgggcgag cgttacggga tcgtactgga gcgggacggc gtgcagggcg cgcgcgccgt
      421 caccgtggtt ccccccgtgc cgggcgtggt cgcggcgccg ggcgagggga cggccatctc
      481 gttcacgaag gacatcaccc tcacgagcct cggtgtgctc gatgccgaga tggtcgtctg
      541 gtacgaggtc gtgaatccca agaccggcct gccggaacgc gaggagatgg cgtacggcct
      601 gccaggcccc tcggcgggcc cggaccgcac cttcacgacg ttcctttccc aggaccgcgc
      661 cgagatcctg cgccgtctgg gcctcggtcc ggaattccgc ggcgtggagc tgctccgcac
      721 gggtgcgcac gtcgagatcc tgagcgcgga atgggattcc gccgcgcccg acccgggcct
      781 ggaggacggg cacggcttct tcgcatcggt gggccggtat gacgtcacgt ggctccttcc
      841 gaccgcggag gtcacgacca tgggcatggt ggaccgacag cagcgctgat tccgtggttc
      901 gcgagccggc ccggggccgc gctccgatcc cgtgcggaat tcgggcgaat agaacccggc
      961 agggggcttc gtggttgacc ccgtctcccg gcaccgatag gttgtgtctc acaccccgaa
     1021 gccaccgaca tgcccaatcc cgacatctgg ttcaacggaa agctcgttcc ccacgaatcg
     1081 gcgaccgtgc acgtgctctc tcacgcgctg cactacggca cttccgtgtt cgagggcatc
     1141 cgatcgtaca agaccgaacg gggatcggcg atcttccgtc tccgggagca catgcagcgc
     1201 ctcgtcgact cggcgcggat ctaccggatg gagattccct attcgctcga ggagctggag
     1261 aaggcggtga ccgacacgat cgagggctcc gggctcgagg cgtgctatat ccgccccctc
     1321 gtgttcaggg gccacggacc gatgggcgtg aacccgctca agaacccggt cgagacggtc
     1381 gtcgccgtat gggagtgggg cgcgtacctg ggcgccgagg ccctggagca gggcgtgaag
     1441 gtggaggtgg ccagctggca gcgcatggca ccgaacacct tcccgtcgct ggccaaggcg
     1501 gcaggcaact acctgaatgc gggcctggtc aagatggacg ccgtgctgaa cggggtcgtc
     1561 gaaggcatca tgctctcgac gaacggcttc gtggccgagg ggtcgggtga gaacctgttc
     1621 ctggtgaagg ggggcgtact gtacacggcg ccggccgcgc tctcgatcct gccggggatc
     1681 acgcgggatt cggtgatgcg gatcgccgcg gacctggggt acgaggtgaa ggagtcgctc
     1741 atcccgcgcg aggccctgta cctggccgac gaggtgttct tcacgggcac cgcggccgag
     1801 gtcacgccgg tgcgcgagat cgaccaccac gtgatcggga gcgggcgcat gggcccggtg
     1861 accgcgcgga tccaggaagc cttcttcgac gtgatccggg cgggcaacga tccgtacggc
     1921 tggctgaccc ccgtcaacgt gggcgccgtg gcctgaggcc ggcgggtcag gtgcgctggt
     1981 ccgtgcggag cttctccagg agctcgctca gccgccggag gtcctcttcc gtgacgtgcc
     2041 tggcgatgcg gacctggagt tcggtgacga cgggtgccat ccgctgaagc agttccagcc
     2101 cggcctcgtt gatgcccacg cggaccacgc gccggtcggt gcgggaccgc gagcggctga
     2161 tgaggtgggc cttgtccagg cggttcaaaa gacgcgtcac gtcgggcgtc cggtccagga
     2221 ggcggccgcc gatctccgag caggtgaccc ggttcgggta ggcgcccgcg aggatccgca
     2281 ggacgttgta ctgggcgggg gtgacaccga agggcgccat cgcggccgac atctcgcccg
     2341 ccacccacga gccggtcacg atcacgttga ggatggcctc gtgaccggcc gagtcgaagt
     2401 ggtcctgcct gatgagatcc tgtagtttca cgcgcgctcc agccggtgga tgatcgttgc
     2461 aacgagcttc taccgggtcg acgccccgcc gttccgcatc cccccgcgga cggtcagtcg
     2521 gccgcgcgga gcgagaatcc gaacgtcgag ccccggccgg gccggctctc gaccacgagg
     2581 tgcgcggcgt gggcggcgag gatgtgcttt acgatggcga ggccgagtcc ggtgccgccc
     2641 tgcgagcgcg agcggctcct gtccacgcgg tagaagcgtt cggtcagccg cgggatgtgg
     2701 tccggatcga tgccgatccc gtcgtccacg atcgagagct tgatgcggcc ccccgccacg
     2761 tgccgggcga cgacctcgac ctggccgtcc ggctcgttgt acttgatcgc gttgtcgacg
     2821 aggttcacga ggacctgccg gagccggtcg gcgtcgccga cggcccgcgg aaggccgacc
     2881 ggaacggtcg tccggagcgt gacgctgcgg tcccgggcaa tcggctcgag cgactccacc
     2941 acctcgcaga ccatcgcgcg caggtcgaag ggcgccatga tcatcgtaag ctcgcccgac
     3001 tcgatccgcg agatctcgcc gaggtcgcgc accaggttgg cgaggcggtc cgagttgcgc
     3061 agaatcttgt ccaggaagga gcggttcacg cgcccgtcgt ccacggcccc cccgagcagg
     3121 gtctcggtga agccccggat cgcgaagatc ggcgtcttga gctcgtgcga gacgttgccc
     3181 aggaattcct tccggtacgt ctccgtcttc cggaggtcct gcatctcgcg ttcgagcacc
     3241 tggccggtcc ggtacacctg ccagaccagg tcgttcagct cgtcgcccct ggggagccgg
     3301 gcggtctcga ggttctcgaa gcggctccgg cggatttcgc gcaggacggt ccgggcgagc
     3361 gtgatccgcc ggccgagcac gacgcccgcg gcgaggtacg caccggcgaa cacgacgggg
     3421 atggcgacga gatggaccgg agccgggccg agcgtggccc acgagagcgc gaccaggggg
     3481 acgccgacca gcgcggtggc aacgagggcc accttggccg cgatccggtg gggcatccgc
     3541 caccgtgacg ggggcggcac gccgatggac gcaggtccct gctccgtccc ggacggggcg
     3601 gaggcgaggc cgtcgccgcc gatgccgtcg ggctcggacg cggacggacc ggctggtcta
     3661 ttcgcggaaa cggtaaccga cacccttgac ggtctcgatg tacgagtcac caatcttctc
     3721 ccggatcttt cgaacgtgaa cgtcgaccgt tcgatccacg acgtacacgt cgcggcccca
     3781 gacttcgtcg agcagctcct ggcgcgagaa cacgcgcccg gcgtttgcgg ccaggaatcg
     3841 gaggagttcg aactccttgc gcggcattcg gatgggtgag tccgacccct cgcgctcgac
     3901 cacgtaccgg agccggtcga tttcgagtcc gtgcgcgtgg accacgtcgg ggatctcctc
     3961 gaagcgctcc gtgccgcgga gcatcgcctt cacctggctc aggaacaccg ggatggagat
     4021 cggcttcgtc aggtagatgt ccgcgccgac gtccagtccc cgcacgtggt cctcctcctc
     4081 gctgcgcgcc gtgagcatca ggacgggcgt cttcttgagc gcgggatgct cgcggatgcg
     4141 cctgcaggcc tcgatgccgt ccatgcgcgg catcatgatg tcgagcacga tgaggtccgg
     4201 gttctcgcgc cgggcaaccg ttatcgcctc ggctccgtcc cgggcctcga cgacgtcgag
     4261 gccggcgtgc tccaggttgt agcggaggag ctccacgatg tcttcctcgt cgtcgacgac
     4321 gaggaccttg gacggagagg gcgccatgat ggtgggtggg ttcagggtcc ctgcagtgcg
     4381 aaggtagtgt tacgccgtta ctgcagtgtt acggggcccg cgggggctga cgcggtcggt
     4441 ccccgccgac gttcgcggtt cgtacattcg ccccgagtcc cactgaacga acaggccatg
     4501 gcactcggca ggaagcttcg gtacggcatg gtgggcgggg gaccgggcgc gttcatcggg
     4561 ggcgtgcacc gcatggcggc cgccctggac ggagggacgg agctcgtcgc gggtgcgttt
     4621 tccgccgacc cggccaagtc ggccgcgacg ggacgggacc tgtacctgga cccgcgccgg
     4681 gtgtacggtt cgtgggccga gatggccgac cgcgaggccg cgcgcgacga cggcatcgac
     4741 ttcgtctcga tcgtcacgcc gaaccacctg catcatgccg tggcccgggc gttcatcgag
     4801 cggggcatcc acgtggtgtg cgacaagccg atgaccaaca cgctggacga ggccgaggac
     4861 ctgtgccggc tcgtcgccga ttcgggtgtg gtcttcgccc tcacgcacaa ctacaccggg
     4921 tacccgatgg tgaaacacgc ccggcacatg gtgggcgccg gcgagctggg caccgtccgc
     4981 aagatcgtgg tcgaataccc gcagggttgg ctggcccggt ccaccgaggg taccggaaac
     5041 aagcaggc
//
LOCUS       DBNHQZ010000122        10340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_983828, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000122 DBNHQZ010000000
VERSION     DBNHQZ010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_983828"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="AC16YU_03095"
     CDS             complement(<1..440)
                     /locus_tag="AC16YU_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR6064237.1"
                     /translation="MRAVLPLLVLALALPFAGCSDEMLVANGERTSDAISPIQADVSS
                     GIGLALIETEIAEAAQSGVFRARLGANEEVATPPVESDAHGIATFRLSRDGSALHYRL
                     IVVGLDNLTASHIHLAPAGQNGGVVAFLYGPALIEGTFSGSLAEG"
     gene            complement(601..1803)
                     /locus_tag="AC16YU_03100"
     CDS             complement(601..1803)
                     /locus_tag="AC16YU_03100"
                     /inference="COORDINATES: protein motif:HMM:NF051539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lnb N-terminal periplasmic domain-containing
                     protein"
                     /protein_id="HIR6064238.1"
                     /translation="MVRRLALFLALATWGGLAQAQIPDTLSDAARISLVTITPGDAVY
                     SMYGHSAVRVSDPPAGVDLVFNYGTFDFSQPWFVARFAYGRLEYYLSVSTVRDALRGA
                     DLENRAVIEQVLDLRPSERDSLYAFLLWNLRPENRRYRYDFFFDNCATRIRDLFEERL
                     GTTLIWTGDPAAGRTLRELLRPYARDRQWLGTGIDLGLGSSTDRPATVREAAFLPDLL
                     ADLVDGGVIGTGADTRPLAPAPPDTVFLPRDVLMAAEPRPWPAWTGWLALLVVVIVTW
                     RERRRHHSSQPVETVDRLVFGLAGVAGLVLGFLVFISEHAVTAPNWNLLWAAPSHVLI
                     AFMPGRAPRNAVTVYYALAAVGAAAVFPAAFLFQSLPAIAFPLAAIVLLRSVRGVLVA
                     RQRPPIEW"
     gene            1914..2786
                     /gene="nth"
                     /locus_tag="AC16YU_03105"
     CDS             1914..2786
                     /gene="nth"
                     /locus_tag="AC16YU_03105"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6064239.1"
                     /translation="MGRRERAQRVLSILTERLGSPTTELEHTSPYELIVSVILSAQCT
                     DERVNRATPALFAEYPDVYALAAAEPEDVLGYVRSISYPSSKARHLVAMARRVRDRFG
                     GRVPESVEDLQTLAGVGRKTALVVASVAHGTDAIPVDTHVFRVAHRLGLVGRDASAPE
                     AVERQLRRTIPREDWSRAHHLLILHGRRTCRARDPQCAECPVSDGCPHLAALEALPGP
                     VPGLDPRRGRCWCGTRHHYFDHPTLRTDRSGVEQAACPRCGSMNVYDSRTGTTLRRVR
                     DPRANTRRRTTRPE"
     gene            2792..4315
                     /gene="gpmI"
                     /locus_tag="AC16YU_03110"
     CDS             2792..4315
                     /gene="gpmI"
                     /locus_tag="AC16YU_03110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR6064240.1"
                     /translation="MRPDQRYLLLILDGYGIAEDPSVSAIDAVRKPYLDHLFATVPHG
                     TLEASGLAVGLPEGQMGNSEVGHMNLGAGRVVYQDITRIDLAVADGSIRSNEVLLAAI
                     DHARSGGGRLHFLGLFSDGGVHSHVDHLVALVELAAARGLGGGDVLVHAFTDGRDTDP
                     HGGVRYVREYEDRVRGLGTGRIATIVGRYYAMDRDRRWERTRRAYDLLVEGVGEAFAS
                     AAEALESSYADGVTDEFVLPRRVEGVDGRVRSGDAIVFFNFRADRGRQLTRAFIDEPF
                     EGFERGRLDNLFYVTLTPYDETFDVHVAFPKVNLARTLGEIISTRGGTQLRAAETEKY
                     PHVTFFFSGGREEPFPGEERILVPSPKVATYDLQPEMSAPELAARVARHLVEHPVNFA
                     VVNFANPDMVGHTGVFEAAVRAVETVDAGAREIVEAARSAGYGVSIIADHGNADRMRN
                     PDGSPHTAHTTALVPHLVIHPDVAGPVRHGKLGDIAPTILHLLGEAIPDEMTGEILV"
     gene            complement(4329..6134)
                     /gene="sppA"
                     /locus_tag="AC16YU_03115"
     CDS             complement(4329..6134)
                     /gene="sppA"
                     /locus_tag="AC16YU_03115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR6064241.1"
                     /translation="MRFLSTLAASTLGTLAAFAIMVVLGLTFLFAMAAASDPAPAVRP
                     GSVLVAELGGTWPESVSGDPLARALGGEAAVDLHDVTRSLRRAAADRRIDAVWLRLGG
                     VTAPWASLQAVRRQIEAVRRAGKPVYATSDTYMVGEKDYFLASAADSVFLDSESLFEF
                     NGFRASVMFYAGLLDRADIRPQAIRAGRFKSAVEPFLRSDLSEEGELQLRALVSDIDD
                     RFVDAVATSRNLDPERVRQLLRDADVGSARDALGAGLIDGLLFADQVEDLLADRLDQD
                     PDERLRTVKLKDYARVTDASAGMPAGREGEIAVVYAEGDIIAGRSGGPALPGVTGSGL
                     GTETFAKAMREARRTASVDAVVVRVNSPGGFAPAADAMRREIELTAAEKPVILSMGDV
                     AASGGYWMATAADTLVAEELTITGSIGVFSLLFDFSGLFERHLGLTFDGVQTGPSADA
                     MSFMRPLTAQETARMERDIDQVYESFLEKVSESRGMTIDQVDSLAQGRVWTGQDALDR
                     GLVDVIGGLSDAIDLAAARAGLAPGSFRVRELPHPPTFLERLAGTMETRMHGFARSLG
                     FGMDPRLEALVAEMGALQRLSGSPQARMAARIEVH"
     gene            6241..6693
                     /locus_tag="AC16YU_03120"
     CDS             6241..6693
                     /locus_tag="AC16YU_03120"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR6064242.1"
                     /translation="MSDYTIPFLHPLVVHVPIALLPAAALALVGWLVRDRIQWLVVAA
                     WLHAAGLAGAVVAMLSGEDLEHEMEGDPMVELFGHTHEDAGQRTVWAAGLLLVLILVS
                     VVWHRRAVLRPGVPAPWRLLVALVGLLVAGLVLWTGHVGGTMVWGVPR"
     gene            6864..8285
                     /locus_tag="AC16YU_03125"
     CDS             6864..8285
                     /locus_tag="AC16YU_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064243.1"
                     /translation="MKSLTHIQSGKVVLVALGLLVSTLAVEPAVARQSERARERTPST
                     RVERRSNTKNDQRATKPSVRTPQESRATAKAPARNERGTAAQQAPARNQRGTAAQQSP
                     SRTGRVQNAPARNERGTAAQQSPSRTGRVDSAPARTGRGTAAQQSPSRTGRVDSAPAR
                     NNGNRGNRVSFPGERRTGKVVFGDRSRLVRRDIRSIITDRTYVVPRYRYRPYVNVWSR
                     PVIRSYWPLIHVHLDWPWLVRYERHWAPRYRYRQVIRVEVYHDGRRDWSNVEVETYYR
                     HSVRRATADWAEIDIEIDQIDVYDGNRYLGYVDRLPGDLGNIRATVYRNGDIRFDREV
                     FLVGDPLVGFEVISTRHYDGWAQDNYRPDDGYRAGRVDLRRHRVQPIRRSRLWDPYRY
                     TGVVPVSLLPDQQGWLWDYGVEALSAVYDDYDYYYGGSGAVAPRGPSEDPGLLYGADE
                     WSYKTRDGADVRYERDSNLERVR"
     gene            8432..9529
                     /gene="hrcA"
                     /locus_tag="AC16YU_03130"
     CDS             8432..9529
                     /gene="hrcA"
                     /locus_tag="AC16YU_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR6064244.1"
                     /translation="MSRANQAETRVQTLSERERTILRLVVQSFVDTAGPIGSRFLSAN
                     FDLGLSPASIRNTMNELEARGFLSHPYTSAGRIPTERGYRAYVDGLTDPARLSRKEQE
                     LVRSHIDEAARDTESLVRESSRLIGQLANLLGVALSPRIATGIMERLEIVPLSSTRLM
                     FVISVKGGLIRTIIMHADSELSRSALDRMVSLLNERLAGLTLEEIRRTCQARVRDLRT
                     EETGIVRLIMDETSMLFSEPPETRQLELGGTGNILSQPEFRAADELRDLVSLLDDEAA
                     VVRLLEDQSQDGGPLARAEVRIGSENMDRDGTERVKRYSVVTAPYQRASMRGTIGVIG
                     PMRMDYARVIAVVEGMAALLSWSPDAGEQAG"
     gene            9576..10268
                     /locus_tag="AC16YU_03135"
     CDS             9576..10268
                     /locus_tag="AC16YU_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6064245.1"
                     /translation="MNRVDKDTNAAGGTGLEDVAEPSPAPETAGREDAAEPTSAPDAA
                     GTEEQLDAADAGDGGEDGAEAPLPGIVDEAPSLEDEVAFLRDRLLRQAAEFQNYRRRT
                     EQEKARMMEFGKAAVVQELLDVVDDFERSIEAAHSAGLGVEEKGSTQAFAAFSDGVGL
                     VYAKLRDALSRAGVEVIEAVGHPFDEHLHEALMRQPAAEGQSPGLVVGEIQKGYRMGD
                     RVLRHAKVIVSA"
BASE COUNT         1589 a         3565 c         3750 g         1436 t
ORIGIN      
        1 ccctccgcca ggctgccgga gaacgttcct tcgatgaggg cggggccgta gaggaaggcc
       61 accaccccgc cgttctggcc ggccggtgcg aggtggatgt gcgaagccgt caggttgtcg
      121 agcccgacga cgatgagccg gtaatggagc gcgctgccgt cccgggacag gcggaaggtg
      181 gcgatgccgt gggcgtccga ttcgacgggc ggtgtcgcga cctcctcgtt cgcacccagg
      241 cgggccctga agacgcccga ctgggccgcc tcggcgatct cggtttcgat gagcgcgagc
      301 ccgatgccgg acgacacgtc ggcctggata ggcgaaatcg cgtccgacgt gcgctcgccg
      361 ttcgcgacga gcatctcgtc ggagcagccg gcgaacggca gggccagggc aagcacgagc
      421 agggggagta ctgcacgcat ggagcacctc tcagtggtga tggggggata gccgggtagg
      481 ccagtcaacg tatgcgactg cgggatgcgc gtcaatccgc ggaggacccg cgcgtagacc
      541 catcattccg agcggtcccc cggcgacgcc gaacggccgg atccggggtg cgagccgccg
      601 ctaccactcg atcggcggcc tctgcctggc cacgagcacg ccccggaccg agcggagcag
      661 gacgatcgcg gccagcggaa acgcgatcgc cggcagcgac tggaagagga aggccgccgg
      721 gaagaccgcc gcggcgccca ccgccgccag cgcgtagtac acggtcacgg cgtttcgcgg
      781 ggcgcgcccc ggcatgaagg cgatgaggac gtggctcggc gcggcccaga gcaggttcca
      841 gttcggggcg gtcaccgcgt gctccgagat gaagaccagg aagccgagca cgaggccggc
      901 cacgccggcc aggccgaaca ccagccgatc caccgtctcg accggctgcg acgagtgatg
      961 tcggcgccgc tccctccacg tgacgatcac cacgaccagg agcgcgagcc agcccgtcca
     1021 ggcaggccag gggcggggtt ccgcggccat aagaacgtcg cgcggcagga acacggtgtc
     1081 cgggggggcg ggagcgaggg gccgggtatc cgcgcccgtg ccgatgaccc cgccgtcgac
     1141 caggtccgcg agcaggtcgg gaaggaaggc ggcctcccgg accgtggcgg gccggtcggt
     1201 cgaggagccg agcccgaggt cgatccccgt cccgagccac tgccggtccc gggcgtacgg
     1261 tcgcaggagc tcgcgcagcg tgcggccggc cgccgggtcg ccggtccaga tcagagtcgt
     1321 ccccagccgc tcttcgaaca ggtcacggat gcgggtcgcg cagttgtcga agaagaagtc
     1381 gtaccggtac ctgcggttct ccgggcgcag gttccacagc aggaaggcgt agaggctgtc
     1441 cctctcggac ggccggaggt ccagcacctg ctcgatgacc gcacggttct cgaggtccgc
     1501 cccgcgcagc gcgtcccgga ccgtcgaaac ggacaggtag tactccaggc gcccgtacgc
     1561 gaagcgcgcg acgaaccagg gctgtgagaa atcgaacgtg ccgtagttga acacgaggtc
     1621 gacgcccgcc ggcggatccg agacgcgcac ggcgctgtgg ccgtacatcg agtagacggc
     1681 gtcgcccggc gtgatcgtca cgagcgagat ccgggcggcg tcggacagcg tgtcggggat
     1741 ctgggcctgc gcgaggcctc cccacgtcgc cagcgccagg aataaggcga gtctccggac
     1801 catgccgttc ggtgtgtcgg attcggctct gcgaagaacc ctattttcgg ccgcggaggg
     1861 gggtgaactc aagctggcgg agcggtttcg cggcaatcta cggaacggag gtcatgggaa
     1921 gacgcgaacg ggcgcagcgg gtgctttcga tcctgaccga gcgactgggg tcgccgacca
     1981 cggagctcga gcacaccagc ccgtatgagc tcatcgtgtc ggtcatcctc tccgcgcagt
     2041 gcaccgacga gcgcgtgaac cgagcgacgc cggcgctctt cgcggagtat ccggacgtgt
     2101 atgccctcgc ggcggccgag ccggaggacg tgctcgggta cgtccgatcc atctcgtacc
     2161 cgagcagcaa ggcccggcac ctggtcgcca tggcccgccg cgtccgggac cggttcgggg
     2221 gacgggtacc ggagtcggtg gaggacctgc agacgctcgc cggggtcggc cgaaagacgg
     2281 cgctcgtcgt cgcgtcggtc gcccacggga cggacgcgat acccgtcgac acgcacgtct
     2341 tccgggtggc gcaccgcctc gggctcgtcg gccgggatgc gtcggcgccc gaggccgtgg
     2401 agcggcagct tcggcgcacg atcccgcgcg aggactggtc ccgggctcat cacctcctga
     2461 tcctgcacgg gcgccggacc tgccgtgcgc gggatccgca gtgcgcggag tgccccgttt
     2521 cggacgggtg tccgcacctc gccgccctcg aggcactgcc ggggccggtc ccgggtctgg
     2581 atccccgtcg cgggcgctgc tggtgcggca cccggcacca ctacttcgac cacccgaccc
     2641 tgcggacgga ccggtccggc gtcgagcagg cggcctgccc acgttgtggt tcgatgaatg
     2701 tgtacgacag ccggaccgga accaccctgc ggcgcgttcg cgatccgcgg gcgaacacgc
     2761 gacgccgcac cacccgaccc gagtgaccac catgcgtccc gaccagcgat acctgctgtt
     2821 gatcctggac ggttacggca tcgccgaaga cccgtccgtg agtgccatcg acgcggtccg
     2881 caagccgtac ctggaccacc tgttcgccac ggtcccgcac ggcacgctcg aggcgtcagg
     2941 cctcgcggtg gggctgcccg agggccagat gggcaactcc gaagtgggcc acatgaacct
     3001 cggcgccggc cgcgtggtgt accaggacat cacccggatc gacctcgccg tggccgacgg
     3061 ctcgattcgc tctaacgagg tgctgctcgc cgcgatcgac catgcccgtt cagggggcgg
     3121 acggctgcat ttcctggggc tgttctcgga cggcggcgtg cattcccacg tcgaccacct
     3181 ggtcgcgctc gtggagctgg ccgccgcccg gggtctcggg ggcggtgacg tgctcgtgca
     3241 cgccttcacc gacggtcgcg acaccgatcc gcacgggggc gtccggtacg tccgcgagta
     3301 cgaggaccgc gttcggggcc tcgggacggg ccggatcgcc acgatcgtcg ggcgctacta
     3361 cgccatggac cgggaccgtc gctgggagcg gacgcgccgg gcgtacgatc tcctggtgga
     3421 gggcgtgggc gaggcgttcg cgtccgccgc cgaggcgctc gaatccagct acgcggacgg
     3481 ggtgacggac gagttcgtcc tgccgcgccg ggtcgagggc gtggacgggc gggtccggag
     3541 cggagacgcc atcgtcttct tcaacttccg ggcggaccgg gggcggcagc tcacgcgcgc
     3601 gttcatcgac gagcccttcg aagggttcga gcgcgggcgg ctcgacaacc tgttctacgt
     3661 cactctcacg ccgtacgacg agaccttcga cgtgcacgtc gccttcccca aggtgaatct
     3721 cgcccggacc ctcggcgaga tcatctcgac gcgcggcggc acgcagctgc gggcggccga
     3781 gacggagaag tacccgcacg tcacgttctt cttcagcggg gggcgcgagg agccgtttcc
     3841 gggcgaggag cgcattctcg tgccgtcgcc gaaggtggcc acctacgacc tgcagccgga
     3901 gatgagcgct cccgagctcg ccgcgcgcgt ggcccgccac ctggtcgagc acccggtcaa
     3961 tttcgcggtc gtgaacttcg ccaaccccga catggtgggc cacacgggcg tgttcgaggc
     4021 cgcggtccgg gcggtcgaga ccgtggacgc gggggcacgc gagatcgtcg aggcggcgcg
     4081 atcggccggc tacggcgttt cgatcatcgc cgaccacggc aacgccgacc gcatgcgcaa
     4141 cccggacggc tcgccgcaca cggcccatac gacggcgctc gtgccgcacc tggtcatcca
     4201 tcccgacgtg gcggggccgg tccgccacgg caaactgggc gacatcgcgc cgacgatcct
     4261 gcacctgctc ggagaggcca tccccgacga gatgacgggc gagatcctgg tctgagcagc
     4321 cggagggctc agtgcacctc gatgcgggcg gccatgcggg cctgggggct gcccgagagc
     4381 cgctggagcg cgcccatctc cgcgacgagg gcctcgagcc gggggtccat gccgaagccc
     4441 aggctccggg cgaagccgtg catccgggtc tccatggtgc cggcgaggcg ctccaggaag
     4501 gtcggaggat gcggaagctc ccgcacccgg aagctgccgg gcgccagccc ggcccgagcc
     4561 gccgccaggt cgatggcatc ggaaaggcct ccgatgacgt ccacgagccc ccgatcgagt
     4621 gcgtcctggc cggtccagac ccgcccctgc gccaggctgt cgacctggtc gatcgtcatg
     4681 ccgcggctct cggacacctt ttccaggaac gactcataca cttggtcgat gtcccgctcc
     4741 atgcgggcgg tctcctgggc cgtgagcggc cgcatgaacg acattgcgtc ggccgagggg
     4801 ccggtctgca cgccgtcgaa ggtcaggccc aggtggcgct cgaagagccc cgagaagtcg
     4861 aagagcaggc tgaagacccc gatcgagccg gtgatcgtca gctcctccgc cacgagggtg
     4921 tcggccgccg tggccatcca gtagccgccg gaggccgcga cgtcgcccat cgagaggatc
     4981 acgggcttct cggccgccgt cagctcgatc tcgcgccgca tggcgtcggc cgcgggagcg
     5041 aagccgccgg gcgaattcac gcggacgacc accgcatcga ccgacgccgt ccggcgggcc
     5101 tcgcgcatcg ccttcgcgaa cgtctcggtg cccaggccgc tgcccgtgac tccgggcagc
     5161 gcggggcctc cgctccgtcc ggcgatgatg tcgccctcgg catacacgac ggcgatctca
     5221 ccctcgcgcc ccgcgggcat gccggccgaa gcgtcggtca cgcgggcata atccttcagc
     5281 ttcaccgtcc ggagccgctc gtcgggatcc tggtccaggc ggtccgcgag gaggtcctcg
     5341 acctggtccg cgaacaggag gccatcgatg agtccggcgc caagggcgtc ccgggcggac
     5401 ccgacgtcgg cgtcgcgcag cagctgccgg acccgctccg gatcgaggtt ccgcgaggtg
     5461 gcgacggcgt ccacgaagcg gtcgtcgatg tcggagacga gcgcccggag ctgcagttcg
     5521 ccctcctcgg acaggtccga ccggaggaac ggctccaccg cgctcttgaa gcgccccgcg
     5581 cggatggcct gcggccggat gtccgcccgg tccagcagcc cggcgtagaa catcacgctc
     5641 gcccggaatc cgttgaactc gaagaggctc tcgctgtcca ggaacaccga gtccgcggcc
     5701 gaggccagga agtagtcctt ctcgccgacc atataggtgt cgctcgtggc gtacacgggc
     5761 tttccggcgc gtcgcacggc ctcgatctgc cgtcgcacgg cctggagcga ggcccacggc
     5821 gcggtcaccc ccccgagcct gagccacacc gcgtcgatgc gccggtcggc ggctgcccgg
     5881 cgcaggctgc gggtcacgtc gtgcaggtcc acggccgcct cgccgcccag cgcgcgcgcg
     5941 agggggtcac ccgaaaccga ctcgggccag gtgccgccca gttcggcgac gagaacgctc
     6001 cccggccgca cggccggtgc ggggtcggag gcggcggcca tggcgaacag gaacgtgagg
     6061 ccgagcacga ccatgatcgc gaatgccgcg agcgtgccga gggtgctggc ggcgagggtc
     6121 gacaggaagc gcatggcgga atcgcgagaa ggggctggag tcgtatcatg gccccgttcc
     6181 gcgggccgag gccgtatacg cgggccctgg ttgatgtatc cccgcgcgcc gggcgaaacc
     6241 atgtccgact acacgatccc gttcctgcac ccgctcgtgg tgcacgtgcc gatcgcgctc
     6301 ctgccggccg cggcgctggc gctcgtgggc tggctggtcc gcgaccgcat ccagtggctc
     6361 gtcgtggccg cctggctcca cgcggccggg ctcgccggcg ccgtcgtggc catgctcagc
     6421 ggggaggacc tggagcacga gatggagggc gatccgatgg tcgagctctt cggccacacg
     6481 cacgaggacg cggggcagcg gaccgtgtgg gcggccgggc tgctgctcgt gctcatcctc
     6541 gtctcggtcg tgtggcaccg ccgcgcggta cttcggcccg gtgtcccggc gccctggcgg
     6601 ctcctcgtgg cgctcgtcgg actcctggtc gccgggctgg tgctctggac cggccacgtg
     6661 ggcggcacga tggtctgggg cgtgcccagg tagtgtccat catcgtggac ggctgaagtc
     6721 tcggggcgac gcctggcggg cgcccggtgc ggcctggagc gggccgggaa agggtccgca
     6781 ttccggcacg gtattgcatg gtttccgggg cagagggtcc ttaccacatg caaaccgcat
     6841 ccagaccgaa ccggaggtgc atcatgaaaa gcctgacaca catccagagt gggaaggtcg
     6901 ttctggttgc gctcggcctg ctcgtctcca cgctcgccgt ggagccggca gtcgccaggc
     6961 agtccgagcg cgctcgcgag aggaccccgt ccacgcgagt ggaacggcgc tcgaacacga
     7021 agaacgacca gcgcgcgacg aagccgtcgg tgcgcactcc gcaggagtcc agggccaccg
     7081 caaaggcccc ggcccgcaac gagcgcggca cggcggcgca gcaggcgccg gcccgcaacc
     7141 agcgcggcac cgcggcgcag cagtcgccga gccggacggg tcgcgtacag aacgcgccgg
     7201 cccggaacga gcgcggcacg gcggcgcagc agtcgccgag ccggacgggt cgcgtggaca
     7261 gtgcgcccgc ccgcaccggg cgcggcacgg cggcgcagca gtcgccgagc cggacgggtc
     7321 gcgtggacag tgcgcccgcc cgcaacaatg gcaaccgggg caaccgggtg tccttccctg
     7381 gcgagcgccg cacgggcaag gtggtgttcg gtgaccgcag ccggctcgtt cgccgggaca
     7441 tccggtcgat catcaccgac cggacctacg tggttccgcg gtatcgctac cgtccgtacg
     7501 tgaacgtgtg gtcccgcccg gtcatccggt cgtactggcc cctcatccac gtgcacctgg
     7561 actggccgtg gctcgtccgc tacgagaggc actgggcgcc ccgctaccgg taccgccagg
     7621 tgatccgcgt ggaggtgtac cacgacgggc ggcgtgactg gtcgaacgtc gaggtggaaa
     7681 cctactaccg gcattccgtg cgccgggcga ccgccgactg ggccgagatc gacatcgaga
     7741 tcgaccagat cgacgtctat gacggcaacc ggtacctcgg gtacgtggac cgcctgcccg
     7801 gcgacctcgg gaatatccgc gcgaccgtgt atcgcaacgg cgacatccgg ttcgaccgcg
     7861 aggttttcct ggtcggcgat cccctcgtgg gcttcgaggt gatctcgacc cggcactacg
     7921 acggctgggc gcaggacaac taccggccgg atgacgggta ccgcgccggc cgcgtggacc
     7981 tgcggcggca ccgggtgcag ccgatacggc gcagccggct gtgggacccc taccggtaca
     8041 cgggtgtcgt gccggtgtcc ctcctgccgg accagcaggg ctggctgtgg gactacgggg
     8101 tcgaagccct gagcgcggtc tacgacgact atgactacta ctacggcggc tcgggggccg
     8161 tggcaccgag gggcccgagc gaggatccgg gcctgctgta cggcgccgac gagtggtcgt
     8221 acaagacccg cgacggggcc gatgtccggt acgagcggga tagcaacctg gaacgcgtcc
     8281 gctgacggat cctccggcgg accgctgggc ggcgcgccgt ggccttcggg ttgcggcgcg
     8341 ccgccgcttt gaagagccgg tgcacgccgc cgctcccgga aacaccaggg ccccggaccc
     8401 ggttgtagcg gggccgatcg agcgtaccga catgtcccgc gcaaaccaag cagaaacaag
     8461 ggttcagacg ctgtccgagc gggagcggac cattctgcgc ctggtcgtgc agagcttcgt
     8521 cgacacggcc gggccgatcg ggtcgcgttt cctgtcggcg aacttcgacc tgggactgag
     8581 ccccgcgtcg atccggaaca cgatgaacga gctcgaggcg cgcggcttcc tgagtcaccc
     8641 gtacacctcg gccggtcgca tccccaccga gcgcgggtac cgggcgtacg tggacgggct
     8701 caccgacccg gcccggctct cgcggaagga gcaggagctc gtgcgttccc atatcgacga
     8761 ggcggccagg gacacggaat cgctggtcag ggagagctcg cgcctcatcg ggcagctcgc
     8821 gaatctcctt ggcgtggccc tgagcccgcg catcgcgacc gggatcatgg agcggctgga
     8881 aatcgtcccg ctctcgtcga cccgcctgat gttcgtcatc agcgtcaagg gagggctgat
     8941 ccggaccatc atcatgcacg cggattcgga gctgtcccgt tccgcgctcg accggatggt
     9001 ttccctgctc aacgagcggc tcgccgggct cacgctcgag gagatccggc gcacgtgcca
     9061 ggcccgggtg cgggacctca ggaccgagga aacgggcatc gtccgcctga tcatggacga
     9121 gacgtcgatg ctgttctcgg agccgcccga gacgcggcag ctcgagctcg gcgggaccgg
     9181 caacatcctc tcgcagcccg aattccgggc ggccgacgag ctgcgcgacc tggtctcgct
     9241 cctcgacgac gaggccgccg tcgtgcgcct tctcgaggac cagtcccagg acggcggtcc
     9301 gctggcccgg gccgaggtcc ggatcggctc cgagaacatg gatcgcgacg gcaccgagcg
     9361 agtaaaacgc tattctgttg tcacggcgcc ctaccagagg gcctccatgc ggggcacgat
     9421 cggcgtgatc ggacccatgc gcatggacta cgcccgggtg atcgccgtgg tcgagggcat
     9481 ggccgctctc ctgagctggt cgcccgacgc cggcgaacag gcgggctgac aggagcggac
     9541 tgccagattc gtcgcaacca gctatccaat tgctcatgaa ccgcgtggac aaggacacga
     9601 acgctgcagg cgggaccggg ttggaggacg tcgccgaacc ctcgcccgcc ccggagacgg
     9661 ccgggcggga ggacgccgcc gagcccacgt ccgctccgga tgcggccggg accgaggaac
     9721 agctggacgc ggccgatgcg ggagacggcg gcgaggacgg ggcggaggcc cctctgcccg
     9781 gaatcgtgga cgaggcaccg tccctcgagg acgaggtggc gtttctccgg gaccggctgc
     9841 tcaggcaggc ggcggaattt cagaactacc ggcgtcggac cgagcaggag aaggcccgca
     9901 tgatggagtt cggcaaggcg gccgtcgtgc aggagctcct ggacgtcgtg gacgatttcg
     9961 agcggtcgat cgaggcggcg cactcggccg ggctcggggt ggaggagaag gggtctacgc
    10021 aggctttcgc cgccttcagc gacggtgtcg ggctcgtcta cgcgaagctc cgggacgcgc
    10081 tctcccgcgc gggcgtcgag gtgatcgaag cggtcggaca cccgttcgac gagcacctgc
    10141 acgaggccct gatgcggcag ccggccgcgg aggggcagtc ccccggtctc gtggtcgggg
    10201 aaatccagaa aggctaccgg atgggcgacc gcgtcctgcg gcacgccaag gtcatcgttt
    10261 cggcctaatg ccggcctcat ccatgcgcga ttactacgag atcctgggcg tcgagcgttc
    10321 ggcatcggcc gactacctga
//
LOCUS       DBNHQZ010000123         3414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3692467, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000123 DBNHQZ010000000
VERSION     DBNHQZ010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3692467"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="AC16YU_03140"
     CDS             complement(<1..809)
                     /locus_tag="AC16YU_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6064246.1"
                     /translation="MPWNLEFAQPFWLWLLPVAVLVPAWRLLRERRSGGLRYSSLGLV
                     RSVRPTLRVRLRQLPTVLRAAALVLGVLALARPRERNVTRETFAEGVDIMMVLDTSTS
                     MRAEDFRPNRFEAARQVGAEFIRGRTSDRIGLIVFAAKAFTQAPLTLDYDFLQAMLSE
                     VEVGVIEDGTAIGTALAMAVNRIKDTEAESKVVILLTDGQNNRGELSPETAAEVAQAM
                     GVRVYTIGVGAYGEAPFVIDHPFAGRQRRMVPVDIDETMLQKVAETTGGRY"
     gene            complement(838..1611)
                     /locus_tag="AC16YU_03145"
     CDS             complement(838..1611)
                     /locus_tag="AC16YU_03145"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HIR6064247.1"
                     /translation="MIQPALILPETLLERLAGATRVAALTGAGVSAESGIATFRDPGG
                     LWARFRPEELAHVDAFLANPERVQGWYAYRRQVVHRAEPNPAHRALARLEALYGDFTL
                     ITQNVDDLHRRAGSVHVVELHGNLTRNYCIACGAAASPGQLDAAPEGPVRCAACGGLV
                     RPDVVWFGETLPEDAVADAWQAASRAEVFLSIGTSGVVYPAAELPLVAARAGAYVVEV
                     NIEPSAVSDAADDVLLGPAGSVLPALVDAVAARLLRTNS"
     gene            complement(1608..2585)
                     /locus_tag="AC16YU_03150"
     CDS             complement(1608..2585)
                     /locus_tag="AC16YU_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064248.1"
                     /translation="MRRIAITTVLSALAAIPASAQVAVRVQADSLSVGEWTELVVAVA
                     HEGVLRAVFPDETGREAPPEAIGIVGDFELLSRLSSGSRTLGDGSRLDSVVYRATTFA
                     LDSAWAAPVVGLATETDTAQVAGVPLLVPVRSVVPPDAQDILDITPIAEFPRTWWPWL
                     ALLAVLAAAILAWRRFLRRPPAEEPEPEPVPEPAEAPLDEALRRLAALARIDVASDEA
                     LKPFFVELSDILRTYVARRTGVPALEMTTGELVRELRGIRRTARLPDERVRDLDRLLQ
                     AADLVKFADLHPAADSARTALHQATATVRIAEQDLGATVAETASDGGAS"
     gene            complement(2587..>3414)
                     /locus_tag="AC16YU_03155"
     CDS             complement(2587..>3414)
                     /locus_tag="AC16YU_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6064249.1"
                     /translation="GLVSNIFGGEYHSAFKGQGMEFAEVRPYQYGDDIRAIDWNVSAR
                     YEDTYVKIFEEEREQTLMLLVDVSGSGDFGARGRFKRELAAELCAVLAFSAVQNNDKV
                     GLILFSDEIELFVPPKKGRRHVLRLVRDLFAHEVHSRGTDLAVALNHVIRVLHRRSIV
                     LLLSDLMADGYESALRAVARRHDTVTFHLVDPTERELPPVGLLDLVDAETGRPMTVDA
                     GAGGTRLAFATTASEADRSVEAVFRRLRVDRVTLRTDEDYVDPLVQFFRYRNRRLSR"
BASE COUNT          525 a         1176 c         1244 g          469 t
ORIGIN      
        1 aaatagcggc ctcccgtcgt ctccgcgacc ttctgcagca tggtctcgtc gatgtccacc
       61 ggcaccatgc ggcgctgccg gcccgcgaac gggtggtcga tcacgaacgg ggcttccccg
      121 taggcgccga cgccgatggt gtacacgcgg acgcccatcg cctgggccac ttccgcggcg
      181 gtttcggggc taagctcgcc gcggttgttc tgtccgtccg tgagcaggat gaccaccttg
      241 ctctcggcct cggtgtcctt gatccggttc acggccatcg cgagcgcggt gccgatggcc
      301 gtcccgtcct cgatgacgcc cacctcgacc tcgctcagca tggcctgcag gaagtcgtag
      361 tcgagggtca gcggcgcctg ggtgaacgcc ttcgccgcga agacgatgag tcctatccgg
      421 tcggacgttc gtccccggat gaactcggcg cccacctggc gggccgcctc gaagcggttc
      481 gggcggaaat cctccgcccg catcgaggtc gacgtgtcca ggaccatcat gatgtccacg
      541 ccctcggcga acgtctcccg cgtgacgttc cgctcgcggg ggcgcgcaag cgccagtacg
      601 cccagcacga gggcggcggc ccggagcacc gtcggcagct ggcggaggcg aacgcggagc
      661 gtcgggcgga ccgagcgcac gagtccgagg gaactgtacc ggagtccccc gctccggcgc
      721 tcgcgcagca gtcgccacgc tggtaccagc acggcgacgg gcagcagcca gagccagaag
      781 ggctgtgcga attcgaggtt ccagggcatc gggaagggga aagcgtgccg ggagagttca
      841 ggaattcgtt cggagaagcc gtgcggcgac ggcgtccacg agggcgggaa ggacggaacc
      901 ggcgggaccg agcaggacat cgtccgccgc atccgagacg gcgctcggct cgatgttcac
      961 ctccacgacg tacgccccgg ctcgcgccgc aacgagcgga agctcggccg ccgggtacac
     1021 gactccgctg gtgccgatcg acaggaagac ctcggcccgg gaggcggcct gccacgcatc
     1081 ggccaccgcg tcctcgggga gcgtctcccc gaaccacacg acgtccgggc ggaccaggcc
     1141 gccgcaggcc gcgcaccgga ccggcccttc gggggccgcg tccaactgcc ccggggaggc
     1201 ggccgccccg caggcgatgc agtagtttcg ggtcagattg ccgtgcagct cgaccacgtg
     1261 gacggagccg gccctccggt gcagatcgtc cacgttctgg gtgatcaggg tgaagtcccc
     1321 gtagagcgcc tccaggcggg cgagcgcgcg gtgcgcaggg ttgggctcgg cccggtgcac
     1381 gacctgccgc cggtatgcgt accagccctg cacgcgctcg gggttggcca ggaaggcatc
     1441 gacatgggcc agctcttccg gccggaaccg tgcccagagg ccgcccgggt cccggaacgt
     1501 ggcgatcccg ctctccgcgc tcacgccggc cccggtgagg gcggccacgc gcgtcgctcc
     1561 ggcgagccgc tcgaggagag tctccggcag gatgagggca ggttggatca tgacgcgcct
     1621 ccgtcggacg ccgtctccgc gaccgtggcg ccgagatcct gctccgcgat gcggacggtg
     1681 gcggtcgcct ggtgcagggc cgtccgcgcg ctgtccgcgg ccgggtgcag gtcggcgaac
     1741 ttcacgaggt cggccgcctg cagcagccgg tccaggtcgc ggacacgctc gtccgggagg
     1801 cgtgccgtcc ggcggatgcc ccggagctcg cggacgagct cgccggtcgt catttcgagg
     1861 gccgggacgc cggtccggcg ggcgacgtac gtgcgcagga tgtccgagag ctcgacgaag
     1921 aagggcttca gggcctcgtc cgaggcgacg tcgatccgcg ccagggcggc caggcgtctc
     1981 agggcctcgt cgagcggcgc ctcggcaggc tcgggcacgg gttccggctc gggctcctcc
     2041 gccggaggtc gccgcaggaa tcggcgccag gcgagaatcg ccgccgcgag gacggccagc
     2101 agcgcgagcc acggccacca cgttcgcgga aactcggcga tcggagtgat atccaggatg
     2161 tcctgggcat cgggcgggac taccgaccgc accgggacca ggagaggaac gccggccacc
     2221 tgcgcggtgt ccgtctccgt ggccaggccc acgaccggcg cggcccacgc ggagtccagt
     2281 gcgaaggtcg tggcccggta cacgaccgaa tcgaggcggg agccgtcgcc cagcgtgcgg
     2341 gagccggaag aaagccgtga cagcagttcg aagtcgccga cgatgccgat ggcctcgggg
     2401 ggcgcctccc gtcccgtctc gtccgggaac accgcgcgca gaaccccctc gtgggcgacc
     2461 gccacgacga gttcggtcca ctcgcccacg gacaggctgt ccgcctggac ccgtacggcc
     2521 acctgggccg aggccgggat cgccgccagg gcggagagaa cggtcgtgat cgcgatgcgc
     2581 ctcatgtcag cgggacaggc gacggttgcg gtagcggaag aactgcacga gcggatccac
     2641 gtagtcctcg tcggtccgga gggtcacccg gtccacgcgc aggcggcgga ataccgcttc
     2701 gaccgaccga tccgcttccg atgccgtcgt ggcgaaggcc agccgggtgc cgccggctcc
     2761 ggcgtcaacg gtcatgggac gtccggtctc ggcgtccacc aggtccagca ggccgacggg
     2821 aggcagctcg cgctcggtgg gatccaccag gtggaaggtg accgtgtcgt ggcgccgggc
     2881 cacggcgcgg agggccgatt cgtatccgtc cgccatgaga tcggagagca ggaggacgat
     2941 ggaccggcgg tgcagcactc ggatcacgtg attgagcgcg acggcgaggt cggtcccgcg
     3001 cgagtgcacc tcgtgcgcga acaggtcccg gaccaggcgc aggacgtgcc ggcggccctt
     3061 cttgggcgga acgaaaagct cgatctcgtc cgagaagagg atgaggccca ccttgtcgtt
     3121 gttctgtacc gcgctgaagg ccaggaccgc gcacagctcg gccgcgagct cgcgcttgaa
     3181 tcgtccgcgg gcgccgaaat cgccggatcc ggagacgtcg acgagcagca tcagggtctg
     3241 ctcgcgctcc tcctcgaaga tcttgacgta ggtgtcctcg tagcgggccg acacgttcca
     3301 gtcgatggcc cggatatcgt cgccgtactg gtacggccgg acctcggcga actccatgcc
     3361 ctgccccttg aaggcggagt ggtactcgcc gccgaaaata ttggagacca ggcc
//
LOCUS       DBNHQZ010000124         2578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3557041, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000124 DBNHQZ010000000
VERSION     DBNHQZ010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3557041"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC16YU_03160"
     CDS             complement(<1..378)
                     /locus_tag="AC16YU_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064250.1"
                     /translation="MRPLLVLLPLLFAACTTSPPEPAGFRAGFGRADITPAIPEPWTD
                     ANGDGRFSAEDGDSFDDRDGDGVFDALWLAGSFEGRAAASVADSLTATALVVDDGRRR
                     IAMVSVDNFGMMLDDVEDVRSRIP"
     gene            complement(501..>2578)
                     /locus_tag="AC16YU_03165"
     CDS             complement(501..>2578)
                     /locus_tag="AC16YU_03165"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6064251.1"
                     /translation="GTGRGPNDTSLPFSKSGYRINNILHSLALELNSRGNRWANRFFT
                     SFNVFRDHRDPFSEDFPTLEIGEDGATYTTAGHEPFSIHNILDQDVLQLTNHFSWFQE
                     KHVITVGVNYESFKFFNSFNIFRHGLFGLPFAATTFGSVDAFLAATDPADPMDLAGMI
                     GSGPFKGEEIHVGQMSLYAQDEYSVSERLRLTFGLRMDMPTYYTDPVPNPYSTSLEAL
                     DENDESETIDQAKFAGATPLFSPRFGFNYDPSGDRSTQLRGGVGIFTGRVPFVWIGNV
                     VSNPGFNPNLYPENQDLVVTRDGESVDPTNLLGGPENDSVLQQSFDLNAMDPDFKWPQ
                     VLTFDLAVDHELGPGLLGTLEAIYSKDINAVVVRNADLVTPVRTLDDGRPYFGGFGAN
                     ELNQAFPFEGAGIYVIDNASDGYNLNVTAQLRKQFAGGLSASLSYAFLESKNLFKSTE
                     IASVLFSESPVQGDPNRPGLSHSEFGNRHRIVGAGTYTHRWSERLATHVGAFVEVAQG
                     NTFLGAGGNRYSFTYSGDVNGDGFGGNDLIYIPTDATDPSEIRFDPSSTRTAAQQAAE
                     FEAFIEQDDYLATHRGEIAERFGAINPWFWNVDLRVMQEVGFVVAGRPQRVQISLDIL
                     NAANLLSSDLGVRKIANPLATSPLVLTRFDDDGEPVFNFTGVDGTYVDDFGLLSRWQL
                     QLGLKYIFN"
BASE COUNT          442 a          822 c          882 g          432 t
ORIGIN      
        1 cgggatgcgc gagcgcacgt cctcgacgtc gtccagcatc atcccgaagt tgtcgaccga
       61 gaccatcgcg atgcggcgcc ggccgtcgtc cacgaccagg gccgtcgccg ttaggctgtc
      121 tgcgacggac gcggccgctc ggccctcgaa cgagccggcc agccacagcg cgtcgaaaac
      181 gccgtccccg tcccggtcgt cgaacgagtc cccgtcctcg gcggagaacc gcccgtcgcc
      241 gttcgcgtcc gtccagggct cgggaatcgc gggcgtgatg tcggcgcggc cgaagccggc
      301 gcggaagcct gcgggttcgg gcggcgaagt agtgcaggcg gcgaagagca ggggcagaag
      361 gacgaggagc gggcgcatgg cgggctggcg atgtggtggt cgggtcatgc ccccaacatc
      421 ggcaagaacc ccgagtctat gaagcgaagg cgccccggac cgcgaagccc ggggcgcccc
      481 cgcaggatcg aacgacggtt tcagttgaag atgtacttga ggccgagctg caattgccag
      541 cgcgaaagca ggccgaagtc gtcgacgtac gtcccgtcca cgccggtgaa gttgaagacg
      601 ggctccccgt cgtcgtcgaa ccgcgtaagg accagcggcg aggtcgcgag cggattcgca
      661 atcttgcgca cgcccagatc gctcgacaga aggttcgcgg cgttcaggat gtccaggctg
      721 atctggacac gctgtggcct gccggccacg acgaacccga cctcctgcat cacccgaagg
      781 tccacgttcc agaaccaggg attgatcgca ccgaagcgct cggcgatctc cccgcgatgc
      841 gtcgccaggt aatcgtcctg ctctatgaac gcctcgaact ccgcggcctg ttgtgccgcc
      901 gtccgggtcg acgacggatc gaagcggatc tccgagggat cggtcgcgtc ggtcgggatg
      961 tagatcaggt cgttgccccc gaagccgtca ccgttcacgt cacccgagta ggtgaacgaa
     1021 taccggttgc cgccggcccc caggaacgtg ttgccctggg cgacctccac gaacgcgccg
     1081 acgtgcgtcg caagccgctc cgaccagcgg tgcgtgtagg tccccgcgcc cacgatacgg
     1141 tgccggttgc cgaactcgga gtgagagagc cccggccggt tcggatcgcc ctggaccggg
     1201 ctctcggaga acagcaccga cgcgatctcg gtggacttga acaggttctt cgactcgagg
     1261 aacgcgtacg acaggctggc ggagagcccg ccggcgaact gcttccgcag ctgcgccgtg
     1321 acgttcaggt tgtacccgtc cgaggcgttg tcgatcacgt agatgccggc gccctcgaac
     1381 gggaacgcct ggttcagttc gttcgccccg aaaccgccga agtacggccg accgtcatcc
     1441 agggttcgga cgggcgtcac gaggtccgcg ttgcgcacta cgacggcgtt gatgtccttg
     1501 ctgtagatcg cctccagagt gccgaggaga cccggtccca gctcgtggtc cacggccagg
     1561 tcgaaggtca ggacctgcgg ccacttgaag tccgggtcca tcgcgttcag gtcgaaagac
     1621 tgctggagca cggagtcgtt ctcgggaccc ccgaggaggt tcgtcgggtc cacgctctcc
     1681 ccgtcgcgcg tcacgacgag atcctggttc tccgggtaca ggttcgggtt gaagcccgga
     1741 ttcgacacca cgttgccgat ccacacgaag ggcacgcggc ccgtgaagat cccgacgccg
     1801 ccccggagct gggtggagcg gtcgcccgag gggtcgtagt tgaatccgaa gcggggggag
     1861 aatagcggcg tcgcaccggc gaacttcgcc tggtcgatcg tctcgctctc gtcgttctcg
     1921 tccagggcct cgagcgacgt cgagtacggg ttcgggaccg ggtcagtgta gtacgtcggc
     1981 atgtccatgc gcaggccgaa cgtcagcctg agccgctcgc tcacggagta ctcgtcctgg
     2041 gcgtagagcg acatctggcc gacatgaatc tcctcgccct tgaatggtcc cgatccgatc
     2101 atgccggcca ggtccatcgg gtccgccgga tcggtcgcgg cgaggaacgc gtcgaccgat
     2161 ccgaaggtgg tcgccgcgaa gggcagcccg aagaggccgt gccggaagat gttgaacgag
     2221 ttgaagaact tgaagctctc gtaattgacg cccaccgtta tcacgtgctt ctcctggaac
     2281 caggagaagt ggttcgtgag ctgcaggaca tcctggtcca ggatgttgtg gatggagaac
     2341 ggctcgtgcc cggccgtcgt gtaggtcgcg ccgtcctcgc cgatctccag cgtcgggaag
     2401 tcttcgctga aggggtcccg gtggtcgcgg aacacgttga agctcgtgaa gaaccggttc
     2461 gcccagcggt tgccgcggct gttcagttcc agggcaagcg agtgcaggat gttgttgatc
     2521 cggtagcccg atttcgagaa cggaagactg gtgtcgttcg ggccgcggcc ggtccccg
//
LOCUS       DBNHQZ010000125         4462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3557273, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000125 DBNHQZ010000000
VERSION     DBNHQZ010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3557273"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="AC16YU_03170"
     CDS             <1..1294
                     /locus_tag="AC16YU_03170"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6064252.1"
                     /translation="VSVVIGSQWGDEGKGKVVDLLSEGMDIVARYQGGANAGHTICWG
                     DRTFVLHLVPSGIFNEGVQCVIGNGVVLDPVAVMDEIGMIREMGYETEGRLFISHNAH
                     LIMPYHKRIEEVRERARDAGAIGTTGRGIGPAYVDKFARTGIRVVDLLDRDVLRKKLK
                     TSIEEKNAILTAVYGAEKLDVEAIIEEYVEFDKLIDPYVTDTVDLLGQALRAGKHVLA
                     EGAQGSLLDVDHGTYPFVTSSHPTAGGACTGLGIPPTEVRRVIGIVKAYSTRVGNGPF
                     PTELTDDVGQRLRDVGHEYGATTGRPRRCGWLDLVALGYSSQINGFTELAITKTDVLS
                     GLANLRVCTDYRLPGGRLSRRFPSDVPTLEGVTPEYADFEGWTEALPGVDRFEDLPDA
                     ARSYLGFIASELGVPIRIVSTGPHRDQTILLEGEDVTA"
     gene            1353..2756
                     /locus_tag="AC16YU_03175"
     CDS             1353..2756
                     /locus_tag="AC16YU_03175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064253.1"
                     /translation="MKAYRVSVNLKLGLIVFAVAIAVASLWYTNDLVDRLREREASMI
                     QLWAEALAQVPKAAQEGALNPYLEEFRLLDEVLTSGVVSDTVGMPLQRDRIDGFRRAV
                     AWAQGMPPSSDLTFILDAFLEPNAFGIPAVLVDSTMQQPIIWRNVDVGAESVAGLSPE
                     DSADVMERLHRVRTRMLAGHDPIPIDVTFPAPGGGPSFRLMQYMLYGESALVTDLRRF
                     PFVQLLFVALFVLVGYLGFSYVRRSEQSSLWVGMAKEAAHQLGTPISSLMGWLEVLRM
                     PGRTAEQEETALDQIGHDIGRLQRVTSRFSDIGSLPRLQEQPVEPVIRQVLDYMQARL
                     PSHRRSVALTADVEDGLVARLNAELFEWVIENLVKNALDAIDSATGEIRLAASRSDGQ
                     IRIDVRDTGKGIDRRSARMIFRPGYSTKKRGWGLGLSLAKRIVEDYHGGTLVLLQSRP
                     GSGSTFRIEIPGTDEAA"
     gene            complement(2753..3970)
                     /locus_tag="AC16YU_03180"
     CDS             complement(2753..3970)
                     /locus_tag="AC16YU_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064254.1"
                     /translation="MAQSATVPHLSEAFSLAQFLASDRSAASDLVIRAYAAAVSEGLG
                     RFGVLRAVVRARGGIGEGRGDGWVDPEAPGPEPADPMVEGSGRDVPVVGVQAGRALRD
                     RLLPSVVGTLDLRDRLVVWFAAAGISSPSDLANVLDTTPDQASEWTRVAEHALSRRLL
                     LAAPLHERLLLIAAENAEPTRDALRRLLDMLLEPAPAALAQHLASTLPSRPGDRARTP
                     GVSGRLRRFVLLLGVIALVAVTGYGIQALRADIEPAPRRAVDAVRRALDLADGAEPVF
                     RTSSAEQAEIFVRDRIGWNVVAPIIQGSPVTGVGLVQALDGVEVPVFFYGDGPGHRVT
                     VVAYTYAFLDRAEPGLYLDRDIRRQIGEPGRFSLLDVGPRRALVFRNRDDIFVAVTPG
                     DAEALRDAIRFPG"
     gene            complement(3974..>4462)
                     /locus_tag="AC16YU_03185"
     CDS             complement(3974..>4462)
                     /locus_tag="AC16YU_03185"
                     /inference="COORDINATES: protein motif:HMM:NF042701.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR6064255.1"
                     /translation="PPDELVVLDRPEYWRSPEPSATFHGRDVFAPVAARLAGGLPLAE
                     VGSPSSGLARLHWALPIFDEQGVRGWVVHVDGFGNCISNIPRGAFEAQRRGRRFKCYV
                     GNTIIDELSPTYGAAAPGEPVLLFDSLDLLEVAVFRGSATEQLEIRAGAPVNLVFTED
                     RA"
BASE COUNT          707 a         1501 c         1612 g          642 t
ORIGIN      
        1 cgtatccgtc gtcatcggaa gccagtgggg agacgagggg aaaggcaagg tcgtcgacct
       61 gctgagcgag ggaatggaca tcgtcgcccg ctaccagggc ggcgccaacg ccggccacac
      121 catctgctgg ggcgaccgga ccttcgtgct gcacctcgtg cccagcggca tcttcaacga
      181 aggcgtgcag tgcgtcatcg ggaacggagt cgtcctggac ccggtcgccg tcatggacga
      241 gatcgggatg atccgggaaa tgggctacga gacggagggc cgcctgttca tctcgcacaa
      301 cgcccacctc atcatgccgt accacaagcg gatcgaggag gtgcgcgagc gggcgcgcga
      361 cgccggcgcg atcgggacga ccggccgcgg catcggcccg gcctacgtgg acaagttcgc
      421 ccggaccggg atccgggtcg tcgatctcct ggaccgggac gtcctccgaa agaagctgaa
      481 gacctctatc gaggagaaga acgccatcct gacggccgtt tacggcgccg agaagctgga
      541 cgtggaggcc atcatcgagg agtacgtgga gttcgacaag ctgatcgatc cctacgttac
      601 cgacacggtg gacctgctcg gccaggcgct ccgcgcgggc aagcacgtgc tggccgaggg
      661 tgcgcagggc tccctgctgg atgtggacca cggcacgtat ccgttcgtga cgtcgagcca
      721 tccgacggcc ggcggcgcgt gcacgggact tggcattccg ccgacggagg tgcgccgcgt
      781 gatcgggatc gtgaaggcgt actcgacccg cgtcggcaac gggccgttcc cgaccgagct
      841 cacggacgat gtcggccagc gccttcgcga cgtgggtcac gagtacggcg ccacgacggg
      901 tcggccgagg cggtgcggat ggctggacct ggtcgcgctc gggtactctt cgcagatcaa
      961 cggattcacg gagctggcca tcacgaagac ggacgtgctg tcgggactgg cgaatctccg
     1021 cgtctgcacc gactaccgtc tgcccggcgg tcgcctgtcc cgccgcttcc cgagcgacgt
     1081 gccgacgctc gagggcgtga ctcccgagta cgcggacttc gaggggtgga cggaggccct
     1141 gccgggcgtc gatcgcttcg aggacctgcc tgatgcggcc cgtagctatc tgggcttcat
     1201 cgcttcggag ctgggagtgc cgatccggat cgtgtcgacg ggaccccatc gcgaccagac
     1261 cattctgctg gagggagagg acgtgaccgc ctgacggtcc acgtgcccag gccgctccgg
     1321 cccgtccgga cactcactgg agggcatcgc ccatgaaggc ctaccgggtt tcggtgaatc
     1381 tcaagctcgg cctgatcgtg ttcgccgtcg cgatcgccgt ggcctcgctg tggtacacga
     1441 acgacctggt cgaccgcctg cgggagcgcg aggcgtccat gatccagctc tgggccgagg
     1501 cccttgcgca ggtcccgaaa gcggcccagg agggcgccct gaatccatac ctggaggaat
     1561 tccggctgct cgacgaggta ctcacctcgg gcgtcgtctc ggatacggtt ggcatgccgc
     1621 tccagcgcga caggatagac ggcttccgcc gcgcggtcgc ctgggcgcag gggatgccgc
     1681 cgtcgagcga tctgaccttc attctggacg cgttcctgga gccgaacgcg tttgggatcc
     1741 cggccgtgct cgtggattcg accatgcagc agcccatcat ctggcgaaac gtggacgtgg
     1801 gcgcggagtc ggtcgccggg ctcagcccgg aggattcggc cgacgtgatg gagcgcctgc
     1861 accgcgtccg gacccggatg ctggccgggc acgatcccat cccgatcgac gtgacgtttc
     1921 cggcgcccgg cggtggcccg tccttccgcc tgatgcagta catgctgtac ggcgagtcgg
     1981 cactggtgac ggacctgcgg cgattcccct tcgtgcagct cctcttcgtg gccctcttcg
     2041 tgctggtcgg ctacctcggg ttctcgtatg tccgccgcag cgaacagagc agcctgtggg
     2101 tgggcatggc caaggaggcc gcccaccagc ttggaacgcc gatatccagc ctgatgggat
     2161 ggctcgaggt gctgcgcatg cccgggcgga cggccgagca ggaggagacc gcgctcgacc
     2221 agatcggtca cgacatcggc cggctgcagc gcgtgacgag ccggttttcg gacatcggat
     2281 cccttccaag gctgcaggag cagccggtcg agcccgtcat ccggcaggtt ctggactaca
     2341 tgcaggccag gctgccgagt caccggcgca gcgtcgccct cacggccgac gtcgaggacg
     2401 ggctcgtcgc ccggctcaac gcggagctgt tcgagtgggt gatcgagaac ctggtcaaga
     2461 acgccctcga cgcgatcgac tcggcgacgg gtgagatccg gctcgccgcc tcccggtcgg
     2521 acgggcagat ccggatcgac gtgcgggaca cgggcaaggg catcgaccga cggagcgcac
     2581 ggatgatctt ccggccgggc tactcgacca agaagcgggg ctggggcctg gggctgtcgc
     2641 tcgccaagcg catcgtggaa gactaccacg gcgggaccct ggtcctgctg cagtcgcgcc
     2701 cgggctcggg ctcgacgttc aggatcgaga ttcccgggac ggacgaggcg gcctagcccg
     2761 ggaagcggat ggcgtcgcgg agcgcctcgg cgtcgccggg ggtgacggcg acgaagatgt
     2821 cgtcccggtt ccggaagacc agcgcccggc gcggacccac gtccagaagg ctgaacctgc
     2881 cgggctcgcc gatctggcgc cggatgtccc ggtcaaggta caggccgggc tcggcgcggt
     2941 ccaggaacgc gtaggtatac gcgacgacgg tcacccgatg gccgggaccg tcgccgtaga
     3001 agaacacggg gacctcgact ccgtccagcg cctgcacgag cccgacccct gtgacggggc
     3061 tgccctggat gatcggcgcc acgacgttcc agccgatgcg gtcccggacg aagatctcgg
     3121 cctgttccgc gctcgaagtc cggaagacgg gctccgcgcc gtcggcgaga tcgagcgcgc
     3181 ggcggacggc atccacggcc cgtcgtggcg ccggctcgat gtcggcgcgc agggcctgga
     3241 tgccgtaacc cgtcacggcg accagggcga tgaccccgag gagaaggacg aagcggcgaa
     3301 gccggccgct tacccccggc gtgcgcgcgc ggtccccggg gcgggagggg agggtcgagg
     3361 cgaggtgctg cgccagggcg gccggcgcgg gctcgagcag catgtcgagc aggcgccgca
     3421 gggcgtcgcg cgtcggctcc gcgttctcgg cggcgatgag cagaaggcgt tcgtgcagcg
     3481 gcgcggccag gagcagccgg cgcgagaggg cgtgctccgc gacgcgggtc cactcggacg
     3541 cctggtcggg cgtcgtgtcc agcacgttgg ccaggtccga aggcgacgag atgcccgccg
     3601 ccgcgaacca caccacgagg cggtcgcgca ggtccagcgt gccgacgacc gacggaagca
     3661 gccggtcgcg caaggcccgc cccgcctgaa cgccgacgac cggcacgtcc cggccgctgc
     3721 cctcgaccat gggatccgcg ggctccgggc ctggggcctc cgggtccacc cacccgtcgc
     3781 cgcggccttc cccgatccct ccccgcgcgc ggacgaccgc acggagcacg ccgaagcgcc
     3841 cgagtccttc ggacaccgcg gcagcatagg cccggatcac gaggtccgac gccgcggacc
     3901 ggtcggatgc caggaactgc gcgagggaaa atgcctcgga cagatggggg accgttgcgg
     3961 attgggccat gcctcaagcg cggtcttcgg tgaaaacgag attcacgggc gcacccgccc
     4021 ggatctcgag ctgttccgtg gcgcttccac gaaacacggc gacctccagg aggtccaggc
     4081 tgtcgaagag cagcacgggc tcgcccggcg cggccgcgcc gtacgtaggg ctgagctcgt
     4141 cgatgatggt gttgcccacg tagcacttga accgcctgcc gcgccgctgc gcctcgaagg
     4201 ccccgcgcgg gatgttcgaa atgcagttcc cgaatccgtc cacgtggacg acccagccgc
     4261 ggacgccctg ctcgtcgaag atcggcagcg cccagtggag ccgcgccagg ccggaagacg
     4321 ggctgccgac ctcggcgagc gggagtcctc cggcgagccg ggccgccacc ggcgcgaata
     4381 cgtcccggcc gtggaaggtg gcgctcggct cgggtgatct ccagtactcg ggacggtcca
     4441 ggacgacgag ctcgtccggt gg
//
LOCUS       DBNHQZ010000126         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1660956, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000126 DBNHQZ010000000
VERSION     DBNHQZ010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1660956"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC16YU_03190"
     CDS             complement(<1..375)
                     /locus_tag="AC16YU_03190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6064256.1"
                     /translation="MDFELTKQQKDIQQAARDFAKGEFDKELALELDAKHEYPTAIWK
                     KAGEEGFIGLHFPVEFGGSGYGCLENVLVVEEFCRRDSSIGAALMLSDFASECILRFG
                     SDEQKKKYLPPVAEGQAISGGAI"
     gene            complement(384..1352)
                     /locus_tag="AC16YU_03195"
     CDS             complement(384..1352)
                     /locus_tag="AC16YU_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6064257.1"
                     /translation="MRKIAVLVDLVDGGVDDSARELATAARAAGGTMVTGFVLGSNVA
                     GTLPELARWFDDVVAVDDAMLAVPDGDVSAAVLAPLLEREAPFCILAAHTNHSMDYVP
                     ALAVRLDRPLVTDCLSVDLGGETVSAVRPAYGGKVHARVSAAASGNGYMATMRPGSVK
                     PGVCPEAAGSVRHEAVPDGISPRRRFVETVSGDAGGVDISQAERLVAVGRGIEDQESL
                     AMIEALAAALGAEVACSRPVVDKQWLPKSRQVGTSGKTVRPRLYLAIGISGSFQHMGG
                     IKGNPFVVAINKDPKAPIFSVADVGIVADLYDVVPLLGEKIQSLKG"
     gene            complement(1356..2132)
                     /locus_tag="AC16YU_03200"
     CDS             complement(1356..2132)
                     /locus_tag="AC16YU_03200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6064258.1"
                     /translation="MDIVVCVKRVPDLSEIEVEVDASRKRIRTADLTFGMNDWDRFAV
                     EEAIRIRDEQGGRVTVLTVGDEESEDVLRRALAMGADEAVRITDPVLAGADAWGTARA
                     LHAAVASIPFDLVLAGSVSSDSGAGLVGGMLAGMLGIPQVALATAITVTGGSMRVRHE
                     VEGGLERVVDVDLPALVTVQTGINEPRYVSIRGIRRVADVAIPVRTAADLGLASGEVR
                     RVELDELYLPPAGGNAEILEGSTESVVDQLIERLRQKGGL"
     gene            complement(2148..>2843)
                     /locus_tag="AC16YU_03205"
     CDS             complement(2148..>2843)
                     /locus_tag="AC16YU_03205"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6064259.1"
                     /translation="IQEIGRAMSRVIEASGEDCGALGRDEVDSGNDVRRIGEEGLFEE
                     LRDRNLEALGSRDFRRIVTNDPHALNALRNDGYDLEQPVLHHSELMAELVRDGKLDLR
                     PIGDDRTYTFHDPCFLGRHNGVYDAPREVLGAVPGLRTVEMERSRNRSFCCGGGLLSL
                     YHEGESETRMGEKRLEMAAAAGAQVVVTACPFCMINLEDAVKTTGRDMEIIDLAELVA
                     RSVGAGTASREDQ"
BASE COUNT          421 a         1042 c          907 g          473 t
ORIGIN      
        1 gatcgcccct cccgaaatgg cctgcccttc ggcgacggga ggcaggtact tcttcttctg
       61 ctcgtcgctc ccgaagcgca gtatgcactc ggaggcgaag tcggagagca tgagcgcggc
      121 cccgatcgag gagtcccgcc ggcagaattc ctccacgacc agcacgttct cgaggcagcc
      181 gtatccggat ccgccgaatt ccaccgggaa gtgcaacccg atgaagccct cctcgcctgc
      241 ctttttccag atcgcggtcg ggtactcgtg cttcgcgtcg agctcgagtg cgagctcctt
      301 gtcgaactcg cccttcgcga agtcgcgcgc ggcctgctgg atatccttct gctgtttggt
      361 cagttcgaag tccatggtac ttgtcagccc ttgagggatt ggattttctc gccgaggagc
      421 ggcaccacgt cgtagaggtc cgcgacgatt cccacgtcgg cgaccgaaaa gatgggcgcc
      481 ttcgggtcct tgttgatcgc caccacgaac gggtttccct tgatgccgcc catgtgctgg
      541 aacgacccgc tgatgccgat cgcgaggtag agcctcggcc ggacggtctt gccggacgtg
      601 cccacctggc ggctcttcgg aagccactgc ttgtcgacca ccggccgtga gcatgccacc
      661 tcggcgccca gcgcggcggc cagggcctcg atcatcgcga ggctctcctg gtcctcgatg
      721 ccccgtccga cggcgacgag ccgctcggcc tggctgatgt cgactccgcc ggcatcgccc
      781 gagaccgtct cgacgaagcg tcgcctgggc gagatcccgt ccgggaccgc ctcgtggcgg
      841 acgctgcctg ccgcttcggg gcagaccccg ggcttcacgg agcccggacg catcgtcgcc
      901 atgtatccgt tgcccgacgc cgcggcgctc accctggcgt ggaccttgcc gccgtacgcg
      961 ggccggaccg cggacacggt ttcgccgccg aggtcgaccg agaggcagtc cgtgacgagc
     1021 ggccggtcca ggcggacggc cagcgccggt acgtagtcca tcgagtggtt cgtgtgcgcg
     1081 gccaggatgc agaacggcgc ctcgcgctcg agaagcggtg ccaggacggc ggccgaaacg
     1141 tctccgtccg gaacggccag catcgcgtcg tcgacggcca cgacgtcgtc gaaccagcgg
     1201 gcgagttccg gaagcgttcc ggccacgttt gagcccagga cgaatcccgt gaccatggtc
     1261 ccgcccgccg cgcgggccgc ggtcgccagt tcccgggcgg agtcgtccac gccgccatca
     1321 acgaggtcga ccagtacagc gatcttccgc atggcctaca gccctccctt ctgcctgaga
     1381 cgctcgatca gctggtcgac gacgctctcg gtgcttccct cgaggatctc cgcgttcccg
     1441 cctgcagggg ggagatagag ttcgtccagc tcgacgcgcc gcacttcgcc cgaggccagg
     1501 ccgaggtcgg ccgccgtccg caccgggatg gcgacgtccg ccacgcgccg gatcccccga
     1561 atcgagacgt accggggctc gttgatgccc gtctgcacgg tcacgagcgc gggcaggtcc
     1621 acgtcgacca cgcgctcgag acctccctcg acctcgtgcc ggacccgcat cgagccgccg
     1681 gtgaccgtaa tcgccgtggc gagcgccacc tgcggaatgc ccagcatccc ggccagcatc
     1741 ccgccgacga gccccgcccc cgaatcgctg gacaccgagc cggccaggac caggtcgaac
     1801 gggatagacg cgacggcggc gtgcagcgcg cgggcggtgc cccacgcgtc cgcccccgcc
     1861 agcaccgggt ccgtgatccg gacggcctcg tccgcaccca ttgcgagggc gcgccggagc
     1921 acgtcctccg actcctcgtc gcccacggtc aggaccgtga cccggccgcc ctgctcgtcc
     1981 ctgatccgga tggcctcctc gacggcgaac cggtcccagt cgttcatgcc gaacgtcagg
     2041 tccgccgtcc tgatgcgttt ccgggaagcg tcgacctcga cctcgatttc cgagaggtcg
     2101 ggcacgcgct tcacgcacac gacaatgtcc atggtgccct gtcttcgtta ttggtcttcg
     2161 cgggatgccg tcccggcgcc gacggaccga gcgacgagct cggccaggtc gatgatctcc
     2221 atgtcgcgcc ccgtcgtctt gacggcgtcc tcgaggttga tcatgcagaa cgggcacgcc
     2281 gtcaccacca cctgcgctcc ggcggccgcg gccatctcga gccgcttctc gcccatcctg
     2341 gtctcgctct cgccctcgtg gtacaggctc agcaggccac cgccgcagca gaagctccgg
     2401 ttccgtgacc gctccatctc gaccgtcctg aggccaggga cggcgccgag aacctcgcgc
     2461 ggagcatcgt acacgccgtt gtgccgcccg aggaagcacg ggtcgtggaa ggtgtatgtc
     2521 cggtcgtcac cgatcggccg gaggtccagc ttcccgtccc gaacgagctc ggccatgagc
     2581 tcagaatgat gcaggacggg ctgctccagg tcgtaaccgt cattccggag tgcgttgagc
     2641 gcgtgcggat cattggtgac gatgcgcctg aagtcccggg atccgagcgc ctcgagattc
     2701 cggtccctga gctcttcgaa gagtccctcc tcgccgatgc gccgaacgtc gttgcccgag
     2761 tccacctcgt cccgacccag cgcgccgcag tcctcgcccg atgcctctat cacgcgcgac
     2821 atggcgcggc cgatctcctg gat
//
LOCUS       DBNHQZ010000127         7059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3286892, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000127 DBNHQZ010000000
VERSION     DBNHQZ010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3286892"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..995)
                     /gene="trmB"
                     /locus_tag="AC16YU_03210"
     CDS             complement(54..995)
                     /gene="trmB"
                     /locus_tag="AC16YU_03210"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR6064260.1"
                     /translation="MILLRRDHLRDGIDVDALYGRPGPLVLEIGFGDGRYLAHLARTR
                     PEWNLLGVEVSLASVSRAFRRMRREGVHTVRIHHGNARFVVRDVLTPSSLHRVIVNFP
                     DPWPRKRHEDNRLLRDDFFRLLSDRLEPEGRVLLTTDHAEYFEFACGQVASTGLFDAE
                     EGDPPDGVLDTKYAEKWKAERRSIRHVAWTVRRSVPPPEPNQLDLPMQHALLAGDLDR
                     VGGFVKQVRRFEGGTAIVTEAFRSLGGDGLLFTVVVEEPDLRQELLVKAWPKEDGVYV
                     GLQAFGDPLGTRGVREAVRAVVDWLVGEGLELRESWI"
     gene            complement(992..4186)
                     /locus_tag="AC16YU_03215"
     CDS             complement(992..4186)
                     /locus_tag="AC16YU_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6064261.1"
                     /translation="MSGSLAYMARNPVAANLLMLVIVLIGLVSLTRIPQETLPEASLD
                     AIQVRMAYPGASPEAVEEGIVRRIEEQIETVEGIRRITAVAAENVGVVTAELKLGTDV
                     ASTLDEIKSEIDRITSFPVDAEEPEVRELVNRRQVIQLAMHGPVAERTLKELADRVKD
                     DLTAMDEISYVRTNGVRDYEIAIEVSREALRAYGLSLSQVALAVRRGSMDLPGGLLRT
                     EGEEILVRTSGQNYTGEDFREIVLLARPDGAAVRLGDVARVTDGFEDADLVTRFDGNP
                     AVLVEVYRSGDERVLDIVERVRGYVASLALPAGVSVDIWNDESKLLKSRFELLVKNGL
                     IGLLLVILALGLFLNTRLAFWVSAGILVSFLGTFIVMSLAGVTVNLISLVAFILALGI
                     VVDDAIVIGENIFAEQERGLPPLDAAVHGVRRMARPVVFAVLTTIAAFAPLLFVPGMI
                     GKFMRNVPAVMIAVLAFSLVESLFILPAHLSRIHAVRGPRRKNAVVRAVESVQARVAR
                     ALDWQISVPLDWAVRFATRHYGLVIVSAVSVALLSVGLVGAGLVHFNFFHDIEGENVV
                     VRLEMPEGTTAARTSEIADRIDAIGQQAIRDLEADLPADHPALLRHRFTTVGITPSDA
                     NSPMAGMLAGLIQPTKAEINFEVLEAERREIGSAAIETAWREAVGTIPGVKSLQFKSS
                     IMTLGKPIQVELSAPSPEVLDAAVDAMKDELVRFAGVVEVEDDRRLGKREIELDLKPG
                     ARPLGLTLDDLARQVRAAYYGDEALRVQRGTDEVRVMVRLPREERDALADLDALRIRT
                     AAGAEVPLAEVATVSTGYGPSSINRRDRRRVTTVTADVDEEVTSASQVIEAMKAEVVP
                     RFESTWPGLRATFEGEQREQSEAFGAIARGFLIALFLIYALLAIPFRSYTQPFIIMSA
                     IPFGIIGAIFGHLVMGISVGVLSMFGIVGLSGVVVNDSLVLIDYINQLRAEGRPVREA
                     VIEATKVRFRPILLTSLTTFLGVLPLILERSLQAQFLIPIAASLGFGILFSTFIVLVL
                     VPALVMLDHRIRSLFGRAEEPAFEHVPAESAGVP"
     gene            complement(4197..5393)
                     /locus_tag="AC16YU_03220"
     CDS             complement(4197..5393)
                     /locus_tag="AC16YU_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6064262.1"
                     /translation="MQSTTLRPFLIGAAILFVGVAGTIGLIALRPDAPRVEAETIAPA
                     VTTVAPEIRTDAIPVAGTGTVRPVSEVQLVAEVGGRIVEVAPGFVSGGRIRQGELLVR
                     IDPADYENAVSMAEAEVMQRGVDSVVAVEEAAIARQEWERVRELTGADAEPATELGSL
                     VLREPQQRMAAAALRAARARLADARTRLARTRVSAPFTGVVRTEQAEVGQVVGPGAPL
                     GVVYAADALEVTVSLLARETELIQGLWSAGRGASGIGARVVVDFGSRPHAWQGTIARV
                     EGALDPATRTVGVVVRVNRPDRSDVPGGPPLLPGMFARAEILAPVTEPYAAIPAEALR
                     ENDTVWIVEGDRLAIRQVEPLYRLDGLVLVRSGLTAADRVVTSSLLAVTDGMAVRASD
                     ATPREP"
     gene            complement(5395..6837)
                     /locus_tag="AC16YU_03225"
     CDS             complement(5395..6837)
                     /locus_tag="AC16YU_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR6064263.1"
                     /translation="MTLRLLFAAALAAVLVAGCSLTPTVARVEAESRLPGTYGSGADS
                     AAASGAARWWTSFGDPVLDAVVDTVLARNRDLAIAALRVAEVQELYRVTRAARLPGAQ
                     LSVEGTRQSTPTNAGATGRFAGNIPGFPDRFDFTTYTASLGLSWELDFWGRVRDATRA
                     AVHDFLATRADLDAARTGIAAEAVATYLEIADVESQLAVARETADLLAERSAAAMDRY
                     QRGLASSFELYRIQQSLADARAAVPQIEAARHDARSRLAVLAGGFPSDLEALLVRAPR
                     LPPLPDPVPAGLPSDLLANRPDLLAAGNRMEAARRRAGVARAERFPRLGLTAAGGTQS
                     SELEGLVSTDQRFGLATGSILLPVFQAGAKSAASRAAMLRYEQAVLAYDRAVLTAFRE
                     ASAALVSLDRQRERSRALASALDDARSSERSQVERFRRGVGDYAALLDARINRLRAEA
                     DAASGARAAALARLTVHRALGGAWTDSDPR"
BASE COUNT         1095 a         2572 c         2443 g          949 t
ORIGIN      
        1 ggctgacgcc gtgacgccgg cgcggatggc cgggacaccg cgcgggctcc ccgtcagatc
       61 cagctctcgc gtagttccag tccctcaccg acgagccagt cgaccaccgc ccggaccgcc
      121 tcgcggacgc cgcgcgtccc cagggggtcc ccaaacgcct gcagtcccac gtacacgccg
      181 tcctccttgg gccaggcctt gaccaggagc tcctggcgca ggtccggctc ctcgaccacg
      241 accgtgaaca gcaggccgtc gccgcccagc gatcggaagg cctcggtcac gatcgcggtc
      301 ccgccctcga aacggcggac ctgcttgacg aatccgccga cgcggtcgag gtcgccggcg
      361 aggagagcgt gctgcatcgg aaggtcgagc tggttgggtt cagggggcgg gacggaacga
      421 cgcacggtcc aggccacatg ccggatcgac cggcgctcgg ccttccactt ctccgcgtac
      481 ttggtatcga gtaccccgtc gggcggatcc ccctcttcgg cgtcgaagag ccccgtcgaa
      541 gcgacctggc cgcacgcgaa ctcgaagtac tcggcgtgat ccgtggtcag gaggacccgg
      601 ccttcgggct ccaggcggtc cgagagcagc cggaagaagt cgtcccggag cagccggttg
      661 tcctcgtgcc gcttgcgcgg ccacggatcc gggaagttga cgatgacgcg gtgcaggctg
      721 ctcggcgtga gcacgtcgcg caccacgaag cgggcgttgc cgtgatggat ccggaccgtg
      781 tgcaccccct ccctccgcat gcgccggaac gcccgggaca ccgaggcgag cgacacctcg
      841 acgcctagca ggttccattc cgggcgcgtc cgggcgagat gcgcaaggta gcgtccgtcc
      901 ccgaagccga tctccagcac gagcggtccc ggccggccgt acagcgcatc gacgtcgatc
      961 ccgtcgcgaa ggtggtcgcg gcgcagcagg atcacgggac gcccgcggat tcggcgggca
     1021 cgtgctcgaa cgccggctcc tccgcccgac cgaagagcga ccggatccgg tggtccagca
     1081 tcacgagcgc cgggaccagc accagcacga tgaacgtgga gaacaggatg ccgaagccca
     1141 ggctcgccgc gatcgggatc aggaactggg cctgcaggct ccgctcgagg atcagcggaa
     1201 gtacgcccag gaacgtcgtg agcgacgtga ggaggatcgg ccggaagcgg accttggtgg
     1261 cctcgatgac ggcctcccgg acggggcgtc cttccgcccg cagctggttg atgtagtcga
     1321 tgagcaccag ggagtcgttg accaccacgc cgctcaggcc cacgatgccg aacatgctca
     1381 gcacgccgac cgaaatgccc atgaccaggt gcccgaagat ggcccctatg atcccgaacg
     1441 ggatggccga catgatgatg aagggctgcg tgtacgagcg gaagggaatc gcgagcaggg
     1501 catagatcag gaacagcgcg atcaggaaac cccgggcaat ggccccgaac gcctcggact
     1561 gctcccgctg ctcaccctcg aacgtcgcgc ggaggcccgg ccaggtgctc tcgaaccggg
     1621 gcacgacctc cgccttcatc gcctcgatca cctgggaagc cgacgtcacc tcctcgtcca
     1681 cgtcggccgt gacggtggtc acccgccgcc ggtcccgccg gttgatggac gacgggccgt
     1741 atcccgtgga aacggtcgcg acctcggcca gcggcacttc ggcacccgcc gcggtccgga
     1801 tccgcagcgc gtccaggtcc gcgagcgcgt cccgctcctc gcgagggagc ctgaccatga
     1861 cccgcacttc gtccgtgccc cgctgcacgc gaagggcctc gtccccgtag taggccgcgc
     1921 gcacctgccg cgccaggtcg tcgagggtca gccccagggg ccgcgcaccg ggcttcaggt
     1981 cgagttcgat ctcgcgcttg cccaggcggc ggtcgtcctc cacctcgacc acgccggcga
     2041 agcggaccag ctcgtccttc atcgcgtcca ccgccgcgtc gagtacttcg ggggacgggg
     2101 ccgaaagctc cacctggatc ggcttgccca gggtcatgat ggacgacttg aactggagcg
     2161 acttcacacc cggaatcgtg cccacggcct cccgccaggc ggtctcgatg gccgccgatc
     2221 cgatctcgcg ccgctcggcc tccaggacct cgaagttgat ctcggccttc gtcggctgga
     2281 tgagcccggc cagcatgccg gccatgggcg agttcgcgtc cgacggcgtg atgcccaccg
     2341 tcgtgaaccg gtgccgaaga agcgcgggat ggtcggccgg aaggtcggct tccaggtcgc
     2401 ggatggcctg ctgaccgatc gcgtcgatcc ggtcggcgat ctcggaggtg cgcgcggcgg
     2461 tcgttccctc gggcatctcc agccgcacga cgacgttctc gccctcgatg tcgtggaaga
     2521 agttgaagtg gacgaggccg gcgccgacga gtcccacgga caggagggcg accgagacgg
     2581 cgctgacgat cacgagcccg tagtgccggg tggcgaaccg cacggcccag tcgagcggga
     2641 cgctgatctg ccagtccagg gcccgcgcga cccgcgcctg caccgattcg acggcccgca
     2701 ccacggcgtt cttccgacgc ggtccccgaa cggcgtggat gcgggagagg tgggcgggca
     2761 ggatgaagag cgactcgacg agcgagaagg cgagcaccgc gatcatcacg gcggggacgt
     2821 tccgcatgaa cttcccgatc atgcccggca cgaagagaag cggggcgaag gccgcgatcg
     2881 tcgtgagcac ggcgaatacc acgggtcgcg ccatgcgcct cactccatgg acggccgcgt
     2941 cgaggggagg caggccccgt tcctgttctg cgaagatgtt ctcgccgatc acgatggcgt
     3001 cgtcgaccac gatgccgagc gccaggatga aggccacgag cgagatcagg ttcacggtga
     3061 ccccggccag cgacatcacg atgaacgtcc cgaggaacga gaccaggatg cccgccgaca
     3121 cccagaaggc aaggcgggtg ttcaggaaca gcccgagcgc gaggatcacc aggagcagcc
     3181 cgatcaggcc gttcttcacc aggagctcga accggctctt gagcagcttc gactcgtcgt
     3241 tccagatgtc gaccgagacg cccgccggaa gcgccagcga cgccacgtag ccccgcaccc
     3301 gctccacgat gtccagcacg cgctcgtcgc cgctgcggta cacctcgacg agcacggcgg
     3361 gattgccgtc gaagcgggtc acgaggtccg catcctcgaa gccgtccgtt acccgggcca
     3421 cgtcgccgag ccgcacggcg gccccgtcgg gccgcgcgag cagcacgatc tcgcggaaat
     3481 cctcgccggt ataattctgt ccgcttgttc ggaccaggat ctcctcccct tccgtcctga
     3541 gcaggccgcc cggcaggtcc atgctgccgc ggcgcaccgc cagggccacc tgggacaggg
     3601 acagtccgta ggcccggagc gcctcgcgcg agacctcgat cgcaatctcg taatcccgga
     3661 ccccgttcgt ccggacgtac gaaatctcgt ccatggccgt caggtcgtcc ttcacccggt
     3721 cggccagctc cttgagcgtg cgctcggcta cggggccgtg catggccagc tggatgacct
     3781 gccgccggtt cacgagctcg cgcacctcgg gctcttccgc gtcgaccgga aaggaggtga
     3841 tccggtcgat ctcggacttg atctcgtcca gcgtcgaggc gacgtccgtg cccagcttga
     3901 gctcggcggt caccacgccc acgttttcgg cggcgaccgc ggtgatgcgc cggatgccct
     3961 cgaccgtctc gatctgctcc tcgatgcgtc ggacgatccc ctcctccacg gcctcgggcg
     4021 aagcgccggg gtacgccatg cggacctgga tcgcgtccag ggaagcctcg ggcagcgtct
     4081 cctgcgggat ccgggtcagg ctgaccaggc cgatcagcac gataacgagc atcaggaggt
     4141 tggccgcgac cgggttgcgg gccatgtagg ccagggatcc actcatgggg cgcgccctac
     4201 ggttcgcggg gagtggcgtc ggaggcccgc acggccatgc cgtcggtgac ggcgagcaga
     4261 ctgctcgtga cgacccggtc ggcggccgtg agcccgctgc gcaccagtac gagcccgtcc
     4321 agccggtaca gcggctcgac ctgccggatg gccaggcggt cgccctccac gatccagacc
     4381 gtgtcgttct cccggagcgc ctcggccggg atggccgcgt agggctcggt gacgggcgcc
     4441 aggatctccg cacgcgcaaa catgccgggc aggagcggcg gaccgccggg cacgtcggag
     4501 cggtccgggc gattcacgcg caccaccacg cccaccgttc gggtggccgg atccagggcg
     4561 ccttctacgc gggcgatcgt tccctgccac gcgtgcggcc tcgagccgaa gtccaccacc
     4621 acgcgggccc cgatccctga ggcgccgcgg ccggccgacc agagaccctg gatcagctcg
     4681 gtctcgcggg ccaggagaga cacggtcacc tcgagggcat cggccgcgta caccacgccg
     4741 agcggcgccc cggggcccac gacctgcccg acctccgcct gctccgtccg cacgacgccg
     4801 gtgaaaggag cactgacccg ggtccgggcc aggcgcgtgc gcgcgtcggc gagccgggcg
     4861 cgggcagccc gcagcgcggc cgcggccatg cgctgctgcg gctcacggag caccaggctc
     4921 cccagctccg tcgccggctc cgcgtcggcg ccggtcagct cccggacgcg ctcccattcc
     4981 tgccgcgcga tcgcggcctc ctccacggcg accaccgaat ccacgccccg ctgcatgacc
     5041 tcggcctcag ccatcgatac ggcgttctcg taatccgccg ggtcgatgcg caccagcagt
     5101 tcgccctgcc ggatgcgccc gcccgagacg aatccgggcg cgacctccac gatgcgtccc
     5161 cccacctcgg cgaccagctg cacctcgctc accgggcgga cggtcccggt gcccgcgacg
     5221 ggaatcgcgt cggtccggat ttcgggagcg accgtggtga cggcgggggc gatcgtctcc
     5281 gcctcgacgc ggggagcgtc cggccggagg gcgatcagtc cgatcgtccc ggccacgccg
     5341 acgaacagga tggcggcgcc gatcaggaac gggcgcaggg tcgtggactg catctcagcg
     5401 tgggtcggaa tcggtccatg cgcccccaag ggcgcggtga acggtgagtc gggcgagcgc
     5461 ggcggcccgt gcgcccgatg ccgcgtcggc ctcggcgcgc agccggttga tgcgcgcatc
     5521 cagcagggcg gcgtagtcgc ccactccccg tcggaagcgc tctacctggg atcgctccga
     5581 actgcgggcg tcgtccaggg ccgaggcgag cgcccgggac cgctcccgct gccggtccag
     5641 ggatacgagc gccgcggatg cttcccggaa agccgtgaga acggcccggt cgtaggccag
     5701 gaccgcctgc tcgtagcgca gcatcgcggc cctcgaggcc gcgctcttcg cgccggcctg
     5761 gaaaaccggc agcaggatcg agcccgtggc gagcccgaac cgctggtccg tggaaacaag
     5821 accctcgagt tcggaactct gcgttcctcc tgccgccgtc aggcccaggc gcgggaaacg
     5881 ctcggcccgc gccacgcccg cgcggcgccg cgccgcttcc atccggttgc cggccgccag
     5941 caggtccgga cggttcgcca ggaggtccga aggcagtccg gccggcacgg gatcgggcag
     6001 cgggggaagg cgcggggccc ggaccaggag ggcctcgaga tccgacggga atccgcccgc
     6061 gagcacggcc aggcgggagc gggcgtcgtg gcgggccgcc tcgatctggg gcaccgcggc
     6121 ccgggcatcg gccagggact gctggatgcg gtacagctcg aacgacgaag ccagtccccg
     6181 ctggtaccgg tccatggccg cggccgagcg ctcggcgagc aggtcggccg tctcgcgcgc
     6241 cacggcgagc tggctttcga catcggcaat ctccaggtac gtggcgaccg cctccgccgc
     6301 gatgcccgtc cgggccgcat cgaggtccgc ccgggtcgcc aggaagtcgt gtaccgccgc
     6361 ccgggtcgcg tcacggaccc gcccccagaa atcgagctcc cacgacagcc cgagcgacgc
     6421 ggtgtacgtc gtgaagtcga accggtccgg aaagcccggg atgttgccgg cgaagcgccc
     6481 cgtggccccg gcgttggtcg gcgtgctctg gcgggtcccc tcgacggaca gctgcgcgcc
     6541 cggcaggcgc gcggcccgcg tcacgcggta cagctcctgt acctccgcga cgcgcagggc
     6601 ggcgatcgcc aggtcccgat tcctcgccag caccgtatcg acgacggcgt ccagcaccgg
     6661 gtccccgaag ctcgtccacc agcgggcagc gcccgacgcg gccgcggagt ccgcaccgct
     6721 cccgtaggtc ccggggaggc gcgactccgc ctcgacgcgg gcgaccgtcg gcgtgagcga
     6781 gcaccccgcg acgaggaccg ccgcgagggc cgctgcgaag agaaggcgaa gcgtcatcgc
     6841 ttagcgaaac cgttgagcga aaggtccgcc agccgcccga accggtcgca aagatccggt
     6901 gaatcacgcg ccctgcgcgg cgccggtcac tcctcggccg gggaggtcgt gcgcagtccc
     6961 agctcgcggt cgatcaggag caggccctcg cccgactcgc ccgccatgcg caggttgtcg
     7021 atgatctcct gggtggtctc ctcttcctcg acctgctcg
//
LOCUS       DBNHQZ010000128         6566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2745189, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000128 DBNHQZ010000000
VERSION     DBNHQZ010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2745189"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC16YU_03230"
     CDS             <1..645
                     /locus_tag="AC16YU_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HIR6064264.1"
                     /translation="ASTAQAAEGAYGTLPPAVVLDVDETVLDNMTYQARLIRDGEYFS
                     GETWASWVEEAAAVPVPGALEFTRAAAARGVSVIYLTNRDAEGEAATRRNLAAFGFPV
                     EDDPDAVLTQGERSEWRPSDKEPRRRYVASGHRVLLLVGDNLGDFITTGRAGLEERTA
                     LYEEHAGWWGTRWIMLPNPQYGSWEAATFGNEYSLGPAERTLRKLDLVRIRAGS"
     gene            693..2105
                     /locus_tag="AC16YU_03235"
     CDS             693..2105
                     /locus_tag="AC16YU_03235"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106219 - zinc ion sensor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR6064265.1"
                     /translation="MRILALIILLLPTASCAQTRGAGSAEPRNVILFIADGCGFATFT
                     MARDYAATVIGREPIFDGILTGSVVTRSSHQRVTDSAAAGTALACGVHTRNGQIATDT
                     TGAPVGTVLEAAERTGYRTGLVTTTRITHATPAAFSAHVIDRALEDEIAVQQLGQEIE
                     VLLGGGMMHFLPAGGGGGRLDGRNLLEEAAAAGYSVVRDAAELEAADAVPLVGIFDPS
                     HVDYEIDRDEGTEPSLAAMTRKAIDLLSDAPGGFFVMIEAGRIDHAAHGNDPAAHLHD
                     LLAYDEAMRVALDFARRDGATLVVATSDHETGGLTVGRDGIYAWHPEALVPVSRSLEV
                     LREHLAARIPGVQDGDEVLAQVVALAEDSGMGPIDDGTRERLRETIRAGLASGASPAA
                     IGDRVDDVLADLLSRRAGLGWTTSGHTAVDVPMYAEGPGSDLFRGTLTNAEFGRRLAR
                     ALGVDLTRVTRDLRAPEAGD"
     gene            2182..3861
                     /locus_tag="AC16YU_03240"
     CDS             2182..3861
                     /locus_tag="AC16YU_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6064266.1"
                     /translation="MTTCVVVDTETTGIRASDDRVIEIGAVWIRDGVIGDRFETLVNP
                     GVSVPWRITRLTGISSRDLVGAPGPAEAWSRFLEFAGDAPLVAHHARFDRGFMEAELL
                     RLGMEPPVRDWICTVKLSRRLLPGLPSHGLDSVARFLAVPVGTRHRALADAEITAGVL
                     LELADRASERGVDDMRGLVALQGARYATASVVPEHVERLRRELVPTLPEAPGVYRLLD
                     ARENILYVGKATSLRDRVGSYFVAVEAKDVRLRELVRRTRAVSVQQTATEIEARVVEA
                     RLIRSLQPPFNRAQREPGRRAWVRVDADQAGGRTIAVRSWRGDDGAAWIGPLSGRGEA
                     QGLAEAIGAVTGLTVERPRPRSGWARESEARLETAARKVSEAAREDPALADWTLEGIP
                     SHARGPIREAMLKASSELAFEEARILRDWLEVLDRLDADGNVADTLTGDVAAVRAGAP
                     VEVVLIRDGRLVGSVAGTGRAVAEGIRDLVGRVPPDGPAAESRVGGLRARRGREDETW
                     MLAQWIVRGGARTVRRDDGEPADAFASRIEEACSQDPPSGDRRTDAGASRP"
     gene            3917..5134
                     /locus_tag="AC16YU_03245"
     CDS             3917..5134
                     /locus_tag="AC16YU_03245"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6064267.1"
                     /translation="MDRPVTLLCLASYFKGARLIEAAKEAGARVILVTNEKLQDAPWP
                     MDSIDERFLLPDVNRQPDVTNAVAFLARTRHIDRILALDEFDTVTGAQLREHLRIPGL
                     GDTTGRYFRDKLAMRMLAREAGIPVPDFVPAINDDELAAFMDRVPGPWLLKPRTEASA
                     MGIRRIEAADALWPILEALGDRRSWFLMEAYVAGDVYHVDSIVSGRRPAFSSVSRYGT
                     PPLDVYQGGGVFVTRTVRRDDEAHAELTRANELLLEAFGMVRGATHAEFIRSRADGTY
                     RFIEVAARVGGAHIDLLVEHATGVNLWAEWARTEIADVRGEPYGVEEDRALSTGLIVC
                     LARDEHPDLSRYDDPEIVWRLRKKHHAGVIVASPDPTRVDELLDRYAARFTDDFLAVK
                     PPLGGAAEMGQLE"
     gene            complement(5136..>6566)
                     /gene="glgA"
                     /locus_tag="AC16YU_03250"
     CDS             complement(5136..>6566)
                     /gene="glgA"
                     /locus_tag="AC16YU_03250"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR6064268.1"
                     /translation="PFAKTGGLADVSGSLPPALAELGCEVKLFMPLYGSIPVIDHGFV
                     FAPELRDLPVPVGGRSARVNVWYGKLPGTDVEVYLVDSPHYFHRGGLYTNDPDEDERF
                     LVLQHAAFAVMQRYAWSPDVIHANDWQAALMPVFLRTSYGWDDLFSGTASLLSIHNIG
                     YQGLFGPHGVHKAGLTYDLFHDGGPLSHNGAFSFLKAGILFATTISTVSETYAHEIQS
                     ERHGAGLDAVLRHRGSDLFGIRNGIDTSAWNPATDPHIAANYTADTLDDKLLNKQALL
                     GRFGLPYEPDVPVLGIISRLTGQKGFDLLQPILGPLLQEEHVQLVVLGSGEPALEDFF
                     NWAQRAFPERVGVYIGYNDPLAHQIEAGADAFLMPSHYEPCGLNQMYSLAYGTVPIVH
                     RTGGLADTVHDWHEMGGHGNGFSFWDATPHALLTTIRRALSLYRTPAAWRELQRRGME
                     GDFSWTGSAEKYLDLYWRSVHRHRGF"
BASE COUNT          942 a         2253 c         2442 g          929 t
ORIGIN      
        1 gcgtccaccg cccaggccgc cgaaggggcg tatggcacgc tgccgccggc cgtcgtcctg
       61 gacgtggacg agaccgtgct cgacaacatg acgtaccagg cgcggctcat ccgcgacggg
      121 gagtactttt ccggggaaac gtgggcctcc tgggtggagg aggcggccgc cgtaccggtt
      181 ccgggtgccc tcgagttcac gcgggctgcc gcggcccggg gcgtgagcgt catctacctc
      241 acgaaccgcg acgccgaggg ggaggcggcg acgcgccgca acctggccgc cttcggtttc
      301 ccggtcgagg acgatccgga cgcggtcctg acccagggcg agcggtccga gtggagaccc
      361 tccgacaagg agccccggcg gcgctacgtc gcgtccggcc accgcgtcct gctcctcgtc
      421 ggcgacaacc tgggtgactt catcacgacc gggcgggcag ggctcgagga gcgcaccgcc
      481 ctctacgaag agcacgccgg ctggtggggc acccggtgga tcatgctgcc gaacccccag
      541 tacggctcgt gggaggcggc gaccttcggc aacgagtact ccctgggtcc ggccgaacgg
      601 accctccgca agctggacct cgtccggatc cgggccggct cctgacacag ccggaccggc
      661 cgtcgacgcc caccgtccga ttcccgacta cgatgcgcat ccttgccctc ataatcctgc
      721 ttctgcccac cgcctcgtgt gcccagaccc ggggtgcggg gagcgccgag ccgcgcaacg
      781 tgatcctgtt catcgcggac ggatgcggat tcgccacgtt cacgatggcg cgtgactacg
      841 cggccaccgt catcggccgc gagccgattt tcgacggcat cctgaccgga tcggtcgtca
      901 cccgatccag ccatcagcgc gtcacggact cggcggcggc cgggaccgcg ctcgcctgcg
      961 gcgtccacac gcggaacggc cagatcgcca ccgacacgac gggagctccc gtcggtaccg
     1021 tgctcgaggc ggccgagcgg accggctacc ggacgggcct cgtgacgacg acccgcatca
     1081 cgcacgcgac gccggccgcg ttcagcgcgc acgtgatcga ccgcgccctg gaggacgaga
     1141 tcgccgtcca gcagctcggc caggaaatcg aggtgcttct gggcggcgga atgatgcatt
     1201 tcctgcccgc ggggggcggt ggcgggcggc tggacgggcg gaacctgctc gaggaggccg
     1261 cggcggccgg gtactcggtg gtccgcgacg cggcagagct ggaagcggcg gatgcggtcc
     1321 cgctcgtcgg gatcttcgac ccgtcgcacg tggattacga gatcgaccgg gacgagggga
     1381 ccgagccgtc gctcgcggcc atgacccgca aggccatcga cctcctctcg gacgcaccgg
     1441 gcggtttctt cgtcatgatc gaggcggggc ggatcgacca cgcggcccac gggaacgatc
     1501 ccgccgcgca cctgcacgac ctgctggcct acgacgaggc gatgcgggtc gcgctcgact
     1561 tcgcgcgccg ggacggggcg acactcgtcg tggcgacctc ggaccacgag acgggcggcc
     1621 tgacggtggg gcgggacggc atctacgcct ggcaccccga ggcgctcgtt ccggtcagcc
     1681 ggtcactcga agtgctgcgg gagcacctag ctgcccgcat cccgggcgtg caggacgggg
     1741 acgaggtgct cgcgcaggtg gtagcgctcg ccgaggattc cggaatgggt ccgatcgacg
     1801 atgggacccg cgagcgcctt cgcgagacca tccgcgcggg actcgcatcc ggcgcgtcgc
     1861 cggccgcgat cggtgaccgg gtggacgacg tcctggccga cctgctctcc cgcagggccg
     1921 gcctcgggtg gacgacctcg ggtcacaccg ccgtggacgt gccgatgtac gcggagggcc
     1981 cgggatcgga cctctttcgc ggcaccctga cgaacgccga attcgggcgg cgcctggccc
     2041 gggcgctcgg ggtggacctc acccgggtga cgcgggacct gcgggccccg gaagccggcg
     2101 actgactcgg cacggggcgc tgcgccatcc ggttgacacc agggcaggat accttcggtc
     2161 acaacgagct ccgaggaagc cgtgaccact tgcgtagtcg tcgatacgga aacgaccggg
     2221 atccgggcgt ccgatgaccg cgtcatcgaa atcggcgcgg tgtggatccg tgacggcgtg
     2281 atcggcgacc ggttcgagac gctggtgaac ccgggggtgt cggtcccgtg gaggatcacg
     2341 cgcctgaccg gcatctcgtc gcgcgacctc gtgggcgccc ccggcccggc ggaggcgtgg
     2401 tcccggttcc tggagttcgc aggcgacgcg ccgctcgtgg cgcatcacgc gcgcttcgac
     2461 cgtgggttca tggaagcgga gctgctccgc ctgggcatgg agccccccgt ccgggactgg
     2521 atctgcacgg tcaagctgtc gcgccgcctg cttccggggc tgccgtcgca cggcctggac
     2581 tcggtcgcgc ggttccttgc cgtgcccgtc gggacacggc accgtgcgct cgcggacgcg
     2641 gagatcacgg ccggcgtgct gctcgagctc gccgaccgcg cgtcggaacg gggcgtcgac
     2701 gacatgcgcg gcctcgtcgc gctgcagggc gcgcggtatg caacggcgag cgtggtcccc
     2761 gagcacgtgg agcggcttcg gcgcgaactc gttccgacgc tgcccgaagc gcccggcgtg
     2821 tatcgcctgc tcgacgcccg ggagaacatc ctgtacgtgg gcaaggcgac ctcgctccgg
     2881 gaccgcgtgg gctcgtattt cgtggcggtc gaggcgaagg acgtgcgcct tcgcgagctc
     2941 gtacgaagga cccgcgcggt gtcggtacag cagacggcca ccgagatcga ggcgcgggtg
     3001 gtcgaggctc ggctgatccg gtcgctgcag ccgccgttca accgggccca gcgcgagccg
     3061 ggcaggcggg cgtgggtccg cgtggacgcg gaccaggcgg gtggcaggac gatcgccgtt
     3121 cgctcgtggc ggggcgacga cggcgccgca tggatcggtc cgctctcggg acgcggagag
     3181 gcccaggggc tcgccgaggc catcggggcc gtgaccgggc tcaccgtgga gcggccccgg
     3241 ccgcggtccg gctgggccag ggaatcggag gcacgcctgg aaacggcggc ccggaaggta
     3301 tcggaagccg cccgcgagga ccccgccctc gcggactgga ccctggaagg gatcccgtcc
     3361 catgcgcgcg gtccgatccg cgaggccatg ctgaaggcct cgtccgagct ggcgttcgag
     3421 gaggcgcgca tcctgcggga ctggctcgag gtcctggatc ggctggacgc ggacggcaac
     3481 gtcgcggaca cgctgacggg cgatgtcgcg gccgtccggg ccggcgcccc ggtcgaggtc
     3541 gtgctcatcc gcgacggccg cctggtcggc tcggtcgcgg ggaccggccg cgccgtggcg
     3601 gaggggatcc gcgatctcgt gggccgcgtc ccgccggacg ggccggcggc cgaatcccgc
     3661 gtgggcggcc tgcgggcccg gcgcggccgc gaggacgaga cctggatgct cgcccagtgg
     3721 atcgttcggg gcggcgcgag gaccgtgagg cgggacgacg gcgaaccggc ggacgccttt
     3781 gcgagccgca tcgaggaggc gtgcagccag gatccgccct ccggtgaccg gcgtacggat
     3841 gcgggcgcgt cgcggccctg accgccgggg catccgtcct gcccaccgcc ggcgccccca
     3901 tcccgaagtc accgccatgg accgtcccgt aaccctcctc tgccttgcgt cctatttcaa
     3961 gggcgcgcgc ctgatcgagg cggccaagga ggccggagcg cgcgtgatcc tggtcacgaa
     4021 cgagaagctg caggatgccc cgtggcccat ggactcgatc gacgagcgct tcctgctgcc
     4081 cgacgtgaac cgccagcccg acgtcaccaa cgcggtcgcg ttcctggccc gcacgcggca
     4141 catcgaccgc atcctcgcgc tggacgagtt cgacacggtg acgggtgcgc agctccggga
     4201 gcacctgcgg attcccggcc tgggcgacac gaccggacgg tacttcaggg acaagctggc
     4261 gatgcggatg cttgcccgcg aggccggcat cccggtgccc gacttcgtgc cggccatcaa
     4321 cgacgacgag ctcgcggcgt tcatggaccg cgtaccggga ccctggcttc tgaagccgcg
     4381 cacggaagcg tcggccatgg gcatccggcg catcgaggcg gcggacgcgc tctggccgat
     4441 cctcgaggcg ctcggggatc gtcggtcctg gttcctgatg gaagcctacg tcgcgggcga
     4501 cgtctaccac gtggattcga tcgtttccgg ccgcaggccg gcgttcagct ccgtgagccg
     4561 gtacggcacc ccgcccctcg acgtgtacca gggcggggga gtgttcgtca cgaggaccgt
     4621 gcggcgggac gacgaggcgc acgcggagct gacgcgcgcc aacgagctgc tgctcgaggc
     4681 gttcggcatg gtccggggcg ccacgcacgc cgagttcatc cggagccgtg cggacggcac
     4741 gtaccggttc atcgaggtcg cggcgcgcgt cggcggcgcc cacatcgacc tcctcgtgga
     4801 gcacgcgacg ggcgtgaacc tgtgggctga atgggcccgc accgagatcg cggacgtgcg
     4861 cggcgagccg tacggcgtgg aggaggaccg ggcgctcagc accgggctca tcgtgtgcct
     4921 cgcccgcgac gagcaccccg acctgtcccg ctacgacgat cccgagatcg tgtggcgcct
     4981 gcggaagaag caccatgcgg gcgtgatcgt ggcgtctccg gatccgacgc gcgtcgacga
     5041 gctcctggac cgctatgcgg cgcggttcac cgacgatttc ctcgccgtga agccgcccct
     5101 gggcggcgcg gccgaaatgg ggcagctcga gtagctcaga agccgcggtg gcggtgcacg
     5161 ctgcgccagt acaggtccag gtacttctcg gccgagccgg tccacgagaa gtcgccctcc
     5221 atgccccgcc gctggagctc ccgccacgcg gccggtgtcc ggtagagcga tagcgcgcgc
     5281 ctgatcgtgg tgagcagcgc gtggggtgtc gcatcccaga acgagaagcc gttcccgtgc
     5341 ccgcccatct cgtgccagtc gtgcacggtg tcggcgagcc cgcccgtccg gtgcacgatg
     5401 gggacggttc cgtaggcgag gctgtacatc tggttcaggc cgcagggctc gtagtgcgag
     5461 ggcatcagga aggcatcggc cccggcctcg atctggtggg cgaggggatc gttgtacccg
     5521 atgtacacgc cgacgcgctc cgggaaggcg cgctgcgccc agttgaagaa gtcctccagg
     5581 gcgggctcgc cgctgccgag cacgacgagc tgcacgtgct cctcctgcag cagcgggccg
     5641 agaatgggct gcagcaggtc gaaacccttc tgcccggtga gccgcgagat gatgccgagc
     5701 accgggacgt ccggctcgta cggcaggccg aaccgcccca ggagggcctg cttgttgagg
     5761 agcttgtcgt ccagcgtgtc ggccgtgtag ttcgcggcga tgtggggatc ggtggccggg
     5821 ttccaggccg aggtgtcgat cccgttccgg atgccgaaca ggtccgatcc ccggtggcgc
     5881 agcaccgcgt cgagtcccgc gccgtggcgc tcggactgga tctcgtgggc gtaggtctcg
     5941 ctgaccgtcg agatcgtggt cgcgaagagg atgcccgcct tcaggaacga gaacgctccg
     6001 ttgtgcgaga gcggcccccc gtcatggaac aggtcgtagg tcagccctgc cttgtgcacc
     6061 ccgtgcgggc cgaagagccc ctggtagccg atgttgtgta tcgacaggag ggaggcggtc
     6121 ccggagaaga ggtcgtccca gccgtaggac gtgcggagga agaccggcat gagggcggcc
     6181 tgccagtcgt tcgcgtggat gacgtcgggc gaccaggcgt accgctgcat cactgcgaac
     6241 gccgcgtgct gcaggaccag gaagcgctcg tcctcgtccg gatcgttcgt gtacaggccg
     6301 ccccggtgga agtagtgggg gctgtccacc aggtacacct cgacgtccgt tcccggcagc
     6361 tttccgtacc agacattcac ccgcgccgag cgcccgccca ccgggacggg gaggtcccgc
     6421 agctcggggg cgaagacgaa gccgtggtcg atcaccggga tcgagccgta cagcggcatg
     6481 aagagcttta cctcgcagcc gagctcggcg agggcgggcg gaagggagcc gctgacgtcg
     6541 gccaggccgc cggtcttggc gaaggg
//
LOCUS       DBNHQZ010000129         4335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3287858, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000129 DBNHQZ010000000
VERSION     DBNHQZ010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3287858"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..2199
                     /gene="thrS"
                     /locus_tag="AC16YU_03255"
     CDS             247..2199
                     /gene="thrS"
                     /locus_tag="AC16YU_03255"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6064269.1"
                     /translation="MSQKITLTFPDGSTREFDRGVTGLDVARSISEGLARAALSIGVD
                     GEVRDLDRPIDDDAKIRIFTWDDPEGRSTYWHSSAHLMAEALEALYPGVKFGIGPPIL
                     DGFYYDVDLGERTLGADDLAQVEAKMAELARRDVPYERSEVSKKEALAYFGDKGDEYK
                     LELIDELEDGTITFYEQGNFTDLCRGPHVRSTGVLKNPKLLNVAGAYWRGDASRKQLT
                     RLYGITFPKKSQLDEYLARLEMAKQRDHRKLGRELELFTFSPKVGSGLPLWLPRGTTV
                     RETLVDFLKREQLKRGYLPVITPHIGRLELYRTSGHYPYYSDSQFPPMLDAEEEGYLL
                     KPMNCPHHTQIYAAKPHSYRELPIRLAEFGTVYRYEQSGELGGLTRVRGFTVDDAHLF
                     CTPEQVKDEFKDVIDLTLMVFRALGFSEFTAQVSLRDPSNREKYVGEDEQWDRAEQAI
                     REAAAEKGLDTVEVIGEAAFYGPKLDFMVRDALGRSWQLGTIQIDYNLPERFDLWYVG
                     EDNQKHRPVMIHRAPFGSLERFIGVLIEHCGGNFPTWLAPVQVAVLPVGQDFTDYAGD
                     VAARLKKAGFRVETDVRNEKIGYKIRGAEVLKVPYMAIVGRREMDGGTISVRRHGEGD
                     LGSMTVDAFATLLLGETESIFQAAGF"
     gene            2332..2850
                     /gene="infC"
                     /locus_tag="AC16YU_03260"
     CDS             2332..2850
                     /gene="infC"
                     /locus_tag="AC16YU_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR6064270.1"
                     /translation="MSNKRVRVNEMIRVPQVRVVDPKGNHGIYAVEDALRMAREQELD
                     LVEISPTAEPPVCKIMDFGKYKYELQKREKEARRKSHQAELKEIRFRPHTDTHDFDFK
                     VKHARNFLEQGHKVRAYVQFRGRDIAYKDHGMEVLARFIQEVADVAKMDQEPRMEGRR
                     MATILSPSKKKA"
     gene            2979..3173
                     /gene="rpmI"
                     /locus_tag="AC16YU_03265"
     CDS             2979..3173
                     /gene="rpmI"
                     /locus_tag="AC16YU_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR6064271.1"
                     /translation="MPKMKSNSGAKKRFTKTASGRLKRRKAYHSHILTKKSPKRKRHL
                     RETTLVDPVDEKRIKRLILA"
     gene            3209..3556
                     /gene="rplT"
                     /locus_tag="AC16YU_03270"
     CDS             3209..3556
                     /gene="rplT"
                     /locus_tag="AC16YU_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR6064272.1"
                     /translation="MPRATNRVASRRRRKRILQMAKGYWGRRSKVYTVAKNAVEKGLV
                     HQYRDRRARKRQFRRLWIARINAGARISGVSYSRMIGALRKADIQLNRKVLADLAMHD
                     PKTFEQVVQAAVR"
     gene            3725..>4335
                     /locus_tag="AC16YU_03275"
     CDS             3725..>4335
                     /locus_tag="AC16YU_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA ligase subunit PheS family protein"
                     /protein_id="HIR6064273.1"
                     /translation="MTAPNTDTDARSALDGLAAAVESDPLRTADDVDAYRIRYLSRRQ
                     GRITELLKRIPDVKPGDRKAFGQAVNDLKRRAEERLAEAASRLAGHDAGADTDLDLTL
                     PGRRPPLGSLHPVTRTLLEIRRVFQSLGFSVAEGPEIEDDWHNFTALNFPPDHPARDM
                     QDTFFVEPPGREHGGILLRTHTSPVQIRVMMAQKPPVRVIVPGR"
BASE COUNT          797 a         1492 c         1386 g          660 t
ORIGIN      
        1 tcctatggat gggaacggaa atcttgtccg aattccactt ccgtcccgct ctccgaggag
       61 tttttaccaa tcccgacacg gatggggccg gcgtgcgggc cgtccgcccc ccgacgtatc
      121 ttttcgccct gccgtgcgtt tgacggcgtt cgccgccgca gcgcgggcac caccccgcaa
      181 catactccac acgacaccat ccaaggggcg acccgtgcat ccgtagcgca cgaacgccat
      241 caagccatgt cacagaagat caccctcacg tttcccgacg gatccacccg cgaattcgac
      301 cgcggggtca cgggccttga cgtggcccgc tcgatatccg agggccttgc ccgcgccgcg
      361 ctgtccatcg gcgtcgacgg cgaggtccgc gatctggacc ggcccatcga cgacgatgcg
      421 aagatccgca tcttcacgtg ggatgatccc gagggacggt cgacgtactg gcactcttcg
      481 gcccacctga tggcggaggc cctggaggcg ctctacccgg gggtgaagtt cggcatcggc
      541 ccgccgatcc tggacggctt ctactacgac gtggacctgg gcgagcggac gctcggggcg
      601 gacgacctgg cccaggtcga ggccaagatg gccgagctcg cgcgccgcga cgtgccgtac
      661 gagcgcagcg aggtctcgaa aaaggaggcc ctcgcgtact tcggcgacaa gggcgacgag
      721 tataagctcg agctcatcga cgagctcgag gacggcacga tcacgttcta cgagcagggc
      781 aacttcaccg acctgtgccg gggaccgcac gtgcgctcga ccggggtact gaagaacccc
      841 aagctgctca acgtggcggg agcgtactgg cgtggggacg cgagccgcaa gcagctgacc
      901 aggctgtacg gcatcacctt ccccaagaag tcgcagctcg acgagtacct ggcgcggctg
      961 gagatggcga agcagcgcga ccaccgcaag ctggggcgcg agctcgagct gttcaccttc
     1021 agccccaagg tgggctccgg cctgccgctc tggctgccca ggggcaccac ggtccgggag
     1081 acgctggtcg acttcctgaa gcgggagcag ctcaagcgcg ggtacctgcc cgtcatcacg
     1141 ccgcacatcg ggcgcctcga gctgtaccgg acgtccggcc actacccgta ttactcggac
     1201 tcgcagttcc cgcccatgct ggacgccgag gaggaaggct acctcctcaa gcccatgaac
     1261 tgcccgcacc acacccagat ctacgcggcc aagccccact cgtaccgcga gctgccgatc
     1321 cgcctggcgg aattcggcac ggtgtaccgg tacgagcagt cgggcgagct gggcgggctg
     1381 acccgcgtgc gcggcttcac ggtcgacgac gcccacctgt tctgcacccc ggagcaggtg
     1441 aaggacgaat tcaaggacgt gatcgacctg acgctcatgg tattccgggc gctcggcttc
     1501 tcggagttca ccgcccaggt gtcgctccgc gacccgtcga accgggagaa gtacgtgggc
     1561 gaggacgagc agtgggaccg ggccgagcag gctatccgcg aggcggcggc cgagaaaggg
     1621 ctcgacaccg tcgaggtgat cggcgaggcc gcattctacg gcccgaaact cgacttcatg
     1681 gtgcgcgacg ccctgggccg cagctggcag ctcggcacca tccagatcga ctacaacctt
     1741 cccgagcgct tcgacctctg gtacgtgggc gaggacaacc agaagcatcg tcccgtgatg
     1801 atccaccgcg cgccgtttgg atcgctggaa cgcttcatcg gcgtgctcat cgagcactgc
     1861 ggcggcaact tcccgacgtg gctcgcgccc gtgcaggtgg ccgtcctccc ggtgggccag
     1921 gacttcacgg actacgccgg cgacgtggcc gcacggctga agaaggccgg tttccgggtc
     1981 gaaacggacg ttcgcaacga aaagatcggc tacaagatcc gcggggccga ggtgctcaag
     2041 gtgccataca tggcgatcgt cggaaggcgc gagatggacg gcggaacgat ctccgtccgg
     2101 cggcatggag agggcgatct cgggtcgatg acggtggacg cattcgccac gctgctgctg
     2161 ggtgagacgg agtccatatt ccaggcagcc ggtttctgat gcaggcgggg gaaaccggcg
     2221 tgccccaagg gaggttaatg gtgattccgt ccgctatgta cagatcggcc cgggtgcggt
     2281 agtttcgggc cgctccttca ctgtttccac gaactcacgc aggtacaagc catttcgaac
     2341 aagagagtcc gcgtcaatga aatgatccgg gtgccgcagg tccgggtcgt cgatcccaag
     2401 ggcaaccacg gcatctacgc cgtcgaggac gcccttcgca tggcgcgaga gcaggaactg
     2461 gacctggtcg agatctcgcc gacggccgag ccgcctgtct gcaagatcat ggacttcggc
     2521 aagtacaagt acgagctgca gaagcgggag aaggaggcgc ggcgcaagtc gcaccaggcc
     2581 gagttgaagg agatccgctt ccgtccccac acggacacgc acgacttcga cttcaaggtc
     2641 aagcacgccc gcaatttcct ggagcagggc cacaaggtgc gcgcctacgt gcagttccgt
     2701 ggccgcgaca tcgcgtacaa ggaccacggc atggaggtcc tggcgaggtt catccaggag
     2761 gtcgcggacg tggccaagat ggaccaggag ccccgcatgg agggccgtcg catggccacg
     2821 atcctgagcc cgtccaagaa gaaggcctga cggcccttca ccgtttttcc cccgcccacc
     2881 cggcgtgtcc tgttgtggcg gggagacgga ttgcctattt ttgcaggctc caacaagccc
     2941 ggaccggctc ccgccaggtc cgaccatcta gagcgatcat gcccaagatg aagtccaaca
     3001 gcggcgccaa gaagcggttc accaagaccg cgtcgggccg tctgaagcgc aggaaggcgt
     3061 accacagcca cattctcacg aagaagagcc cgaagcgcaa gcggcacctt cgcgagacga
     3121 cgctggtcga tccggtggac gagaagcgca tcaagcgcct gatcctcgcc tgacgcgacc
     3181 gcacgaatcg tagacttttc gttccgacat gccccgcgca accaatcgag tcgcttcccg
     3241 ccgccgccgc aagcggattc tccagatggc caagggctac tggggccgcc gcagcaaggt
     3301 gtacaccgtc gccaagaacg cggtcgagaa gggtctcgtc caccagtacc gcgatcggcg
     3361 cgcccgcaag cgccagttcc gtcggctctg gatcgcccgg atcaacgccg gcgcccgcat
     3421 cagcggcgtc tcgtactccc ggatgatcgg tgccctccgt aaggccgaca tccagctgaa
     3481 ccgcaaggtc ctcgccgatc tcgcgatgca cgatccgaag acgttcgagc aggtcgtcca
     3541 ggccgccgta cgatgagccc catcccgatg cacacgctcc ggacacgacg attttttggc
     3601 cggtacgcct cgctcctgta ggcagcggta ccggccccgg accatccgtg atcggttcga
     3661 aatcccgccc ctgtcctgca ggcccggcgg gattttcgtt ttgaggcccc ttccggaaac
     3721 caccatgacc gccccgaaca ccgacaccga cgcccgttcc gccctcgacg ggctggccgc
     3781 cgccgtggag agcgacccgc tccggacggc ggacgacgtc gacgcctacc gcatccggta
     3841 cctgagccga cgccagggcc gcattaccga gctcctgaag cgcatccccg acgtcaagcc
     3901 cggggaccgg aaggccttcg ggcaggcggt caacgacctg aagcgccgcg ccgaggaacg
     3961 cctcgcggag gccgcttcgc ggttggccgg ccacgacgcg ggcgccgaca cggacctgga
     4021 cctcacgctt cccggccgcc ggccgccgct cggctccctg caccccgtca cgcggacgct
     4081 cctcgagatc cggcgcgtgt tccagtctct cgggttctcg gtcgccgagg ggcccgagat
     4141 cgaggacgac tggcacaact tcaccgcgct gaacttcccg cccgaccacc cggcccgcga
     4201 catgcaggac acgttcttcg tggagccgcc cggtcgcgag cacggcggca tcctgctgcg
     4261 cacccacacg tccccggtcc agatccgggt gatgatggcg cagaagcccc ccgtacgggt
     4321 catcgtcccg ggccg
//
LOCUS       DBNHQZ010000130         3157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_714025, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000130 DBNHQZ010000000
VERSION     DBNHQZ010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_714025"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="nrfD"
                     /locus_tag="AC16YU_03280"
     CDS             <1..582
                     /gene="nrfD"
                     /locus_tag="AC16YU_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6064274.1"
                     /translation="STVSGLWWIIEIAALRNLVPMVLAETIREPVFWIGVPLALGAAV
                     YTAFLFGQAEGRDLWQNGLLPAHLVVQAAVAGAGALLAMHWVWPVEASVVAVVRPVFI
                     SAVAVDLLLLAGEVWMPHASDTAALAAHEITHGRYRGRFRLGAIGLGHVVPLALVLAP
                     GPWGAGLAGLAAIAGLYAYEYCFVMAPQEVPNS"
     gene            776..>3157
                     /locus_tag="AC16YU_03285"
     CDS             776..>3157
                     /locus_tag="AC16YU_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6064275.1"
                     /translation="MTTVTHPDGRMSQYPPPDRWDDWVEWDGKEWPRRVARRYTLVPT
                     VCFNCESACGLLAYVDRETLDIRKFEGNPVHPGSRGRNCAKGPATINQTYDPERILYP
                     LKRVGARGEGRWKRIRWEEALGEIAGKMRASRLERRDGIMYHVGRPGEDHYANRCITA
                     WGVDGHNSHTNICSAGARAGYAFWSGFDRPSPDHANARVILLISSHLETGHYFNPHAQ
                     RIIEAKQKGAKIITFDPRLSNTASKSDVWLPTWPGSEQTVLLAIANHLIRNDLYDREF
                     VRRWVNWRETLDFFRTRPYEEVEVELPDAGGRGLGERIREIFAGERFDRASDQADFET
                     FDRLLKALYAEFTFERAAEEARVPVERIREAAELVADCQGRLAAHTWRSASIGNLGGW
                     QVARCLFFLNVLTGSVGTRGGTSGNSWNKFVPKPFDTPPAFDAWNELHLPHEWPLAFF
                     EMSFLLPHFLEEGRGTVDVYFTRVYNPMWINPDGFMWLKALRDEEKMKCHVALTPTWN
                     ESAWFADYVLPMGHAGERHDLMSQETHAGQWIAFRQPVRRVAMERLGRPVRFTYEANP
                     GEVWEEAEFWVELSGRMDPDGSLGIRRHFESPYRPGELITQDEYWGWIFENSVPGLPE
                     AAAKEGLTPLAYMRKYGCFEVKRENYLPYEAAVETKGANVDRDGRVVRDGQVVGILVD
                     GEAKVGFTTPSRKLEFFSPTLRDWGWAEKPYTIPWTLESHVHPDRIDVEKGEMLLLPN
                     WRLPTLIHTRSANAKWLYELSHRNPVWMHPEDARRLGVVTGDLVRVTTQIGYFV"
BASE COUNT          492 a         1070 c         1132 g          463 t
ORIGIN      
        1 tcgacggtgt ccggactctg gtggatcatc gagatcgctg cgctccggaa cctggtgccc
       61 atggtgctcg cggagaccat ccgggagccc gtcttctgga tcggcgtgcc gctggcgctc
      121 ggcgccgcgg tgtacaccgc cttcctgttc gggcaggcgg aggggcgcga cctgtggcag
      181 aacggcctcc ttcccgccca cctggtggtc caggccgccg tcgcgggcgc aggcgccctg
      241 ctcgcgatgc attgggtgtg gccggtcgag gcctccgtcg tcgccgtggt gcggccggtc
      301 ttcatctcgg cggtcgcggt cgacctgctg ctgctcgcgg gcgaagtgtg gatgccgcac
      361 gcgtccgaca ccgcggccct cgcggcgcac gagatcacgc acggcaggta ccggggccgc
      421 ttccggctcg gcgccatcgg gctgggacac gtggtgccgc tcgccctggt ccttgccccc
      481 ggcccgtggg gcgcgggtct ggccggcctc gccgccatcg ccggcctgta cgcctacgaa
      541 tactgcttcg ttatggcgcc ccaggaagtc cccaattcct gacgtccccg acgttcaaac
      601 ccccgatgcc atggccacct catcgcaccg catcgccggc ctcctgggac gcctgatgca
      661 ggcggacgcc ccgcctgccc gcggcacgct ggtcgagccg ccggccgccg ccacggaagc
      721 ggagttcgga cttccgcgct tcgccggcac ggaccgggag ccggaaaagg tcccgatgac
      781 gaccgtcacc caccccgacg gccgcatgag ccagtatccg ccgcccgacc ggtgggacga
      841 ctgggtggag tgggacggaa aggagtggcc gcggcgcgtc gcgcggcgct acacgctggt
      901 gcccacggtc tgcttcaact gcgagtcggc ctgcgggctg ctcgcctacg tggaccgcga
      961 gacgctcgac atccgcaagt tcgagggcaa cccggtccac cccggaagcc gcggccggaa
     1021 ctgcgccaag gggccggcga ccatcaacca gacgtacgac ccggagcgca tcctgtatcc
     1081 gctcaagcgc gtgggcgcgc ggggcgaggg ccgctggaag cggatcaggt gggaggaggc
     1141 gctgggcgag atcgccggga agatgcgcgc gagccgcctg gaacgccggg acgggatcat
     1201 gtaccacgtg gggcggccgg gcgaggacca ctatgcgaac cggtgcatca cggcgtgggg
     1261 cgtggacggg cacaacagcc acacgaacat ctgctcggcg ggcgcgcgcg ccggatacgc
     1321 gttctggtcg ggcttcgacc ggcccagccc ggatcacgcc aacgcgcgcg tgatcctgct
     1381 catctcgagc catctcgaga ccgggcacta cttcaacccg cacgcgcagc gcatcatcga
     1441 ggcgaagcag aagggcgcga agattatcac gttcgacccg aggctctcga acaccgcctc
     1501 gaagtccgac gtttggctgc cgacctggcc gggctccgag cagacggtgc ttctcgcgat
     1561 cgccaatcac ctgatccgga acgacctgta cgaccgcgaa ttcgtgcggc gctgggtgaa
     1621 ctggcgcgag acgctcgact ttttccggac gcggccctac gaggaagtgg aggtcgaact
     1681 gccggacgcg ggcggccggg ggctcggcga gcggatccgc gagatcttcg ccggcgagcg
     1741 gttcgaccgg gcgtcggacc aggccgactt cgagacgttc gaccggctcc tgaaggcgct
     1801 ctacgcggag ttcaccttcg agcgggcggc cgaggaggcc cgggtcccgg tcgaacggat
     1861 ccgcgaggcg gcggagctcg tcgcggactg ccagggccgg ctcgccgcgc acacctggcg
     1921 cagcgcgtcc atcggaaacc tgggcggctg gcaggtggcc aggtgcctgt tcttcctgaa
     1981 cgtgctcacg gggtcggtcg ggacccgcgg cgggacgagc ggcaacagct ggaacaagtt
     2041 cgtgccgaag cccttcgaca cgccgcccgc cttcgacgcc tggaacgagc tgcacctgcc
     2101 gcacgagtgg ccgctggcgt tcttcgagat gagcttcctc ctcccccatt tcctggagga
     2161 agggcgcggc acggtcgacg tctacttcac gcgggtctac aacccgatgt ggatcaaccc
     2221 ggacggcttc atgtggctga aggccctccg ggacgaggag aagatgaagt gccacgtcgc
     2281 gctcacgccc acgtggaacg aatcggcctg gttcgccgac tacgtgctgc ccatgggcca
     2341 tgcgggcgag cggcacgacc tgatgagcca ggagacgcat gccgggcagt ggatcgcctt
     2401 ccggcagccg gtccggcggg tcgccatgga gcggcttggc cggccggtgc gcttcacgta
     2461 cgaggccaac cccggagagg tgtgggagga agcggagttc tgggtcgagc tctccggccg
     2521 gatggatccg gacggctcgc tcggcatccg caggcacttc gagagcccct accggccggg
     2581 cgagctcatc acgcaggacg agtactgggg gtggattttc gagaacagcg tgccgggcct
     2641 cccggaggct gccgcgaagg agggcctcac gcccctcgcc tacatgcgga agtacggctg
     2701 tttcgaggtg aagcgggaga attacctgcc gtacgaggcg gccgtcgaga cgaagggagc
     2761 gaacgtggac cgggacgggc gcgtggtccg ggacggccag gtggtcggaa tcctggtgga
     2821 cggcgaggcg aaggtgggtt tcacgacgcc gagccggaag ctcgagttct tctcgcccac
     2881 cctgcgggac tggggctggg cggagaagcc atacacgatt ccctggacgc tcgagtcgca
     2941 cgtgcacccg gatcgcatcg acgtcgagaa gggcgagatg ctgctcctgc cgaactggag
     3001 gctgccgacg ctcatccaca ctcggagcgc caacgccaag tggctctacg agctcagcca
     3061 ccggaacccc gtctggatgc accccgagga cgcccggcgc ctgggcgtcg tgaccggcga
     3121 cctcgtccgg gtgacgacgc agatcgggta cttcgtc
//
LOCUS       DBNHQZ010000131         8767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_577600, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000131 DBNHQZ010000000
VERSION     DBNHQZ010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_577600"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /gene="glgB"
                     /locus_tag="AC16YU_03290"
     CDS             complement(<1..1792)
                     /gene="glgB"
                     /locus_tag="AC16YU_03290"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR6064276.1"
                     /translation="MAWLTSDDLRRWREGRHFEAYRRLGAHVGRGGTWFATWAPHAES
                     VAVIGDFNGWDSSAHPMRRAGSGEEGLWEVFVRGAGAGHHYKFRIHRGPYRADKTDPY
                     AEAMEAPVSEGNPVAGLSSIVVAGGYEWGDGEWMASRRGPASLERPVSIYEVHIGSWM
                     RRSDGYSLSYRDVAEPLADYVKKMGFTHVELLPVQEHPYYASWGYQVVGYFAPTFRYG
                     RPDDFRFLVDTLHRHGVGVILDWVPAHFATDPQGLVFFDGSTLYEYDDPRMRYHPDWG
                     TFVFDYNKPGVRNFLVSNANFWLDRYHIDGLRFDAVASMLYRDYSRHDWIPNHHGGRE
                     NLEAIDLLKTTNVAVYERHPEVLMIAEESTAWPGVSRPTYDGGLGFLYKWNMGWMHDT
                     LTYMRKDPIHRKHHLHDFTFPLVYAWSENYALPLSHDEVVHGKGSLWGKMPGDPWQKA
                     ANLRLLLAHQWGHPGKKLLFMGGEFGQRAEWNHDGGLQWELLAERLHGGIHEWTRALN
                     ALYREQPALWNDRPGGFEWIDFADWEHSVASYLRKPADAEAGAGAVAFIFNFTPVPRD
                     RYRVGLPTAGRWRVLLNSDDRQFGGSGMSPG"
     gene            1948..4092
                     /gene="glgX"
                     /locus_tag="AC16YU_03295"
     CDS             1948..4092
                     /gene="glgX"
                     /locus_tag="AC16YU_03295"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR6064277.1"
                     /translation="MTISQRVHPGRPYPLGATWDGLGVNFALYSQHAERVEVVLFHDD
                     SPDSASRSVVLPERTGPVWHGYVPDLTPGQLYGYRVHGPYRPEDGHRFNPSKLVLDPY
                     ARALGRPPRWHDSLFGYDLKSPDRDLSFDGTDSAPYAPLGVVVAGGYAWEADRSPRIP
                     WEDTVIYETHVRGMTIRHPGVPEDRRGTYLGMSEAVVIDHLKRLGVTTVQLLPVHAKA
                     TEAAIAEAGLSNYWGYNTLAYFAPEPTYAANPAQAVREFKTMVRRLHTAGLEVIIDVV
                     YNHTAEGNRFGPTLSFRGIDNLAYYKEDPDNPRFLIDYTGTGNTLDAGNPHVLQLIMD
                     SLRYWVEEMHVDGFRFDLAAALARDLFEINMLSAFFQVVQQDPVLSQVKLIAEPWDVG
                     PGGYQVGSFPWLWTEWNGKYRDAVRRFWKGDTGVLGEFATRIAGSSDLYGNSGRRPFA
                     SVNFVTAHDGFTLDDLVSFDRKHNEANIEENRDGTDANYSDNCGVEGPTDDPAILARR
                     AARKRALLATLFLSQGVPMVLGGDEISRTQHGNNNAFCQDNAISWFDWSGAKDRQSLI
                     GLLERLAAFRRAHPNFRRHRFLTGAPDEHGRRDVTWWHPAGHEMRMADWQRSGGHVLG
                     ALLRGDRIEDHDAIGNRIEDSTYLVCFNASEKPARFVLPPNGVGNPEAWVVALSSING
                     SLEGGRHRPGAPLELPAVSVTVLEAVIRAAPD"
     gene            complement(4099..5637)
                     /gene="malQ"
                     /locus_tag="AC16YU_03300"
     CDS             complement(4099..5637)
                     /gene="malQ"
                     /locus_tag="AC16YU_03300"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR6064278.1"
                     /translation="MDSTRQSGILLHVTSLPSPFGIGDLGPSAYRFADFLVEAGQTLW
                     QVLPLVPVGYGYSPYSSPSTFAGSPLLISPERLVADGLLEPGDLQEVPDFPKEWVDFQ
                     RVIPYKEALLHLAFERFEAGLSPIAPSDFKAFCRRHADWLDDYALFEAVKAEMGGVEW
                     TAWPRPIASREPSAVARARKDHATAIRMFRFWQYLFDRQWKDLRAYCNRHGIRIFGDL
                     PIYVAQDSADVWSNPDLFHLDASGQGTVVAGVPPDYFSETGQRWGNPIYRWGVMKRNG
                     YAWWIRRMARILDQVDLVRLDHFRGFEAYWEVPATEETAVKGRWVKGPGAKLFKAMEA
                     ELGTLPVVAENLGVITKGVTDLMERFGFPGMAILQFAFDSGPENEFLPHNYTRSLVAY
                     TGTHDNDTFRGWWTDTRSTQEAAVIERAHRYCRAYLDLGAGEEGEIGWHAIRALMGSV
                     AARVVIPLQDVLGYGGEARMNTPGEESGNWGWRFAEGAVRAAHARRLRHLAELYGRLP
                     VAAG"
     gene            5844..6599
                     /locus_tag="AC16YU_03305"
     CDS             5844..6599
                     /locus_tag="AC16YU_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064279.1"
                     /translation="MQAYLESLDPADLHFLVGLVESPVTLTNDAQLRSLAAQMDAGDP
                     DARTRLAAALDREIRYLGSSDVAYAFRSLTGSEPGVSAGEILRDVSGQLKVPHPGLGT
                     FEDQLAHLAEAYATRAFADLPAHEQQRMLEELGVERDKAAAVLKRSAGDFALPVMIEA
                     FNVVVVQGLIKTIIFGTIARIIGRQLAARLFAFLAGRVPWWVGWIGPAAWTLSVGWTA
                     LDLQGPAARKTVPVMLYLGLCAVRKRNVPVSAA"
     gene            complement(6586..8169)
                     /locus_tag="AC16YU_03310"
     CDS             complement(6586..8169)
                     /locus_tag="AC16YU_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR6064280.1"
                     /translation="MMHSERSRGAGMPAEPENGDPGGPERTGQVEPEHRLHPMTLVLR
                     AAVSIPALVFLLLPVFRSPDSSALFNLVLSFLYALVVLPWLVLHYVRFRYRLTPDEVV
                     IHSGVLTRRRRNIPVDRIQNIEIEQQLLQRLTGTARVRIYTAGTQEAEGALDVVSLAE
                     AHRIRQTVRGYQRAVVAAPESSPKADEETGVPAAPSEVLLAMDPKRVLLSGAFRFSLL
                     YIAIIFTFLQYVEPDPEVLVSWLTRGRLAGLARAATASPWAAAAIGIVVAVLVGWVSG
                     IAVNFNRFYRFVLTLEKDRLHRKHGLLSVREGTIPLHRVQALIVRTNPLMRRFGWYTL
                     DVQTMGTDVREQGHSVAIPFGRLGEIQAVIARIREGMPDARTEPWAFPQSLESVSRLT
                     IRRALVRYTLGLAAATGVAVILTDSSAWLLLSLWPLALGAAVLRWRCMGYDLHDRHLV
                     VRRGVLQQYTWIIPVGRLQVLQTRASLFQRHLDLCTVYVDTAGASPVRGADIIDLGRG
                     PAGRLADEIYGQFSVLTPR"
     gene            complement(8156..8665)
                     /locus_tag="AC16YU_03315"
     CDS             complement(8156..8665)
                     /locus_tag="AC16YU_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR6064281.1"
                     /translation="MPDGGIPDPDRGSRMNRLDPRVRKVWAIKLAVAGAVALLAAVIH
                     DVANVFDAERWMPLGVVPGVVLVAFSAILFWVPRLRYRFWWFALTEQELVLERGILNR
                     VRTIVPLRRVQHLDVSQDVVEREFELGKLVVHTAGTRSSEVVVPGLRLEEAERLRDRI
                     KAYIVDDAL"
BASE COUNT         1455 a         3149 c         2867 g         1296 t
ORIGIN      
        1 cgcccggcga catgcccgat ccgccgaact gccggtcgtc gctgttcagc agcacccgcc
       61 accgcccggc ggtcggcagg cccacccggt accggtccct cggcaccggg gtgaagttga
      121 agatgaacgc gacggctccc gccccggctt ccgcgtcggc gggcttgcgc aggtaactcg
      181 cgacggagtg ctcccagtcc gcgaagtcga tccactcgaa tccgccaggg cggtcgttcc
      241 atagcgccgg ctgctcccgg tagagcgcgt tcagcgcccg cgtccattcg tggatccccc
      301 cgtgcagccg ttcggcgagc agctcccact gcagcccgcc gtcgtgattc cattccgccc
      361 gctgcccgaa ctcgccaccc atgaacagca gcttcttgcc cgggtggccc cactggtggg
      421 ccaggagcag gcgcaggttc gcggccttct gccacgggtc gcccggcatc ttgccccaga
      481 gcgagccctt gccgtgcacg acctcgtcgt gcgagagggg cagcgcgtag ttctccgacc
      541 acgcatacac gagcgggaag gtgaagtcgt gcaggtggtg cttccggtgg atcgggtcct
      601 tgcgcatgta cgtcagcgtg tcatgcatcc agcccatgtt ccacttgtac aggaagccca
      661 ggccgccgtc gtacgtcggg cgggagaccc ccggccacgc ggtcgactcc tcggcgatca
      721 tcaggacttc ggggtgccgc tcgtacacgg ccacgttggt cgtcttcagg aggtcgatcg
      781 cctccaggtt ttcgcgaccg ccgtggtggt tcggaatcca gtcgtgccgt gagtagtcgc
      841 ggtagagcat cgaggcgacg gcgtcgaagc gcagcccgtc gatgtggtac cggtccagcc
      901 agaagttggc gttcgagacc aggaaattgc gcacgcccgg cttgttgtag tcgaacacga
      961 acgtgcccca gtccgggtgg tagcgcatgc gcggatcgtc gtactcgtac agggtggacc
     1021 cgtcgaagaa gacgagcccc tgcggatccg tggcgaaatg cgccggcacc cagtccagga
     1081 tgaccccgac cccgtgccgg tgcagggtgt cgaccaggaa ccggaagtcg tcggggcggc
     1141 cgtagcggaa ggtcggcgcg aagtacccga ccacctggta gccccacgag gcgtagtacg
     1201 gatgctcctg gaccgggagg agctccacgt gggtgaagcc catcttcttg acatagtccg
     1261 cgagaggctc cgccacgtcc cggtacgaga gcgaataccc gtccgatctc cgcatccacg
     1321 atccgatgtg gacttcgtag atcgataccg gccgttcgag tgacgcggga ccccggcgcg
     1381 aggccatcca ttccccgtcg ccccactcgt agccgcccgc gaccacgatg gacgagaggc
     1441 ccgccaccgg gtttccctcc gaaacgggcg cctccatggc ctcggcgtac ggatccgtct
     1501 tgtcggcgcg gtaagggccc cggtggatcc ggaacttgta gtggtgcccg gctcccgcgc
     1561 cccgcacgaa cacctcccac aggccctcct cccccgagcc cgcccggcgc atcggatgcg
     1621 cggacgagtc ccatccgttg aaatcgccga tcacggcgac cgactcggca tgcggggccc
     1681 aggtcgcgaa ccaggtgccg ccccggccca catgggcccc gagtcgccgg taggcctcga
     1741 aatgccggcc ttcccgccag cgccggaggt cgtcggatgt cagccaggcc atggcaccct
     1801 cccgaaatgg tggcgcaagg tacgaagcgc ggcgcgcgtg tccctccgaa gaccccgagg
     1861 atccggccgg atggccttgg cgccgccccg accgcccgct atcttccgcc cccggttccg
     1921 atacacacgt tttcgcgcgc ggtcttcatg acgatcagtc aacgggtcca tccggggcgg
     1981 ccctatccgc tcggagcgac ctgggacggg ctgggggtca acttcgccct ctacagccag
     2041 cacgccgagc gggtcgaggt cgtgcttttc cacgacgact cccccgattc ggcgtcccgg
     2101 tcggtcgtcc tgccggagcg cacgggcccg gtgtggcacg ggtacgtgcc cgacctgacc
     2161 ccggggcagc tctacggtta ccgcgtgcac ggcccctacc ggcccgagga cgggcaccgg
     2221 ttcaatccct ccaagctcgt tctcgatccg tacgcccggg cgctgggccg tcctccccgg
     2281 tggcacgaca gcctgttcgg gtacgacctg aagagcccgg accgggacct gtccttcgac
     2341 ggaaccgact cggcgcccta cgcgccgctc ggcgtggtgg tcgcgggcgg atacgcgtgg
     2401 gaagccgacc gctcaccccg catcccttgg gaggatacgg tcatctacga gacgcacgtg
     2461 cggggcatga ccatccggca ccccggcgtg ccggaggacc ggcgcggcac gtacctgggg
     2521 atgagcgagg cggtcgtcat cgaccacctg aagcggctcg gcgtcacgac cgtgcagctc
     2581 ctgcccgtcc acgcaaaggc taccgaggcg gcgatcgccg aggcgggcct ttcgaactac
     2641 tggggataca acacgctggc ctacttcgcg cccgagccca cctacgcggc caaccccgcg
     2701 caggccgtgc gcgagttcaa gaccatggtc aggcggctgc acacggcggg cctcgaggtc
     2761 atcatcgacg tcgtgtacaa ccacacggcc gagggcaacc gcttcggacc cacgctgtcc
     2821 ttccgcggca tcgacaacct cgcctactac aaggaagacc cggacaatcc gcgtttcctg
     2881 atcgattaca cggggaccgg caacacgctc gacgccggca atccgcacgt gctgcagctg
     2941 atcatggaca gccttcggta ctgggtcgag gaaatgcacg tggacggctt ccgcttcgat
     3001 ctcgcggccg cgctcgcccg cgacctcttc gagatcaaca tgctgtccgc cttcttccag
     3061 gttgtccagc aggacccggt gctcagccag gtcaagctga tcgcggagcc ctgggacgtg
     3121 ggaccgggcg gataccaggt cggctcgttc ccctggctct ggaccgaatg gaacggcaag
     3181 taccgggacg cggtccggcg cttctggaag ggcgacacgg gcgtccttgg cgagttcgcc
     3241 acccggatcg ccggctcgag cgacctctac ggcaactccg gccggcgccc gttcgcttcc
     3301 gtcaacttcg tcacggccca cgacggcttc acgctcgacg acctcgtcag cttcgaccgc
     3361 aagcacaacg aggccaacat cgaggagaat cgcgacggca ccgacgccaa ctacagcgac
     3421 aactgcggcg tggaagggcc gaccgacgat ccggcgatcc ttgcccgacg cgcggcccgg
     3481 aagcgggccc tgctcgcgac cctcttcctg tcgcagggag tcccgatggt gctcgggggc
     3541 gacgagatat cccggacgca gcacggcaac aacaacgcct tctgccagga caacgccatc
     3601 agctggttcg actggtccgg cgcgaaggac cggcagagcc tgatcggcct cttggaacgg
     3661 ctggccgcct tccgcagggc ccatccgaac ttccgccggc accgcttcct gaccggcgct
     3721 cccgacgaac acgggcggcg cgacgtgacg tggtggcatc cggcggggca cgagatgcgc
     3781 atggccgact ggcagcgctc gggcggacac gtgctcggcg cgctcctgcg cggggaccgg
     3841 atcgaggatc acgatgccat cgggaatcgg atcgaggact cgacctatct tgtctgcttc
     3901 aacgcctcgg agaagccggc ccggttcgtc ctgccgccca acggcgtggg caaccccgag
     3961 gcgtgggtcg tggcgctctc gagcatcaac ggatcgctgg agggcggacg ccaccggccc
     4021 ggtgcccccc tggagcttcc cgccgtcagc gtgaccgttc tcgaggccgt gatccgcgcc
     4081 gcaccggact gagccggatc acccggccgc gaccgggagg cgaccgtaca gctcggccag
     4141 gtgacgaagc cggcgggcat gcgccgcgcg gacggcgccc tccgcgaacc gccaccccca
     4201 gttccccgat tcctcgccgg gcgtgttcat gcgggcctcg ccgccgtacc ccaggacatc
     4261 ctggagcggg atcaccacgc gggcggccac cgatcccatg agggcgcgga tcgcgtgcca
     4321 gccgatctcg ccctcctcgc ccgctcccag gtccaggtac gcccggcagt agcggtgcgc
     4381 ccgctcgatg accgccgcct cctgggtgga acgcgtgtcg gtccaccagc cgcggaacgt
     4441 gtcgttgtcg tgcgtgcccg tgtaggcgac caggctccgg gtatagttgt gcggaaggaa
     4501 ttcgttctcc ggaccgctgt cgaaggcgaa ctgcaggatg gccatgcccg gaaacccgaa
     4561 ccgctccatg aggtcggtca cgcctttcgt gatcactccc aggttctcgg ccacgaccgg
     4621 cagcgtgccg agctccgcct ccatcgcctt gaacagcttc gcgccgggtc ccttcaccca
     4681 gcggcccttc acggcggtct cctcggtggc gggcacctcc cagtacgcct cgaagccgcg
     4741 gaagtggtcc agccggacca agtccacctg gtccaggatt cgcgccatcc gccggatcca
     4801 ccaggcgtac ccgttccgct tcatcacgcc ccaccgatag atgggattgc cccagcgctg
     4861 cccggtctcg gagaagtagt ccgggggcac gccggccacc accgtcccct gccccgaggc
     4921 gtccaggtgg aacaggtcgg ggttcgacca gacgtccgcg ctgtcctgcg ccacgtagat
     4981 cgggaggtcg ccgaaaatcc ggatcccgtg ccggttgcag tacgccctca ggtccttcca
     5041 ctggcggtcg aagaggtact gccagaaccg gaacatgcgg atggccgtcg catggtcctt
     5101 gcgggcccgg gcaaccgcgg acggctcgcg cgacgcgatc gggcgcggcc atgcggtcca
     5161 ctccacgccg cccatctcgg ccttgacggc ctcaaacagc gcgtaatcgt cgagccagtc
     5221 ggcgtgccgc ctgcagaagg ccttgaaatc cgacggggcg atcggcgaaa ggccggcctc
     5281 gaagcgttcg aacgccaggt gcaggagcgc ctccttgtag gggatcacgc gctggaaatc
     5341 gacccactcc ttcgggaaat ccggcacctc ctgaaggtcg cccggctcca ggagaccgtc
     5401 cgcgaccagc cgctcggggc tgatgagcag cgggcttccg gcgaaggtcg acggcgagga
     5461 gtacggcgag tagccgtagc ccaccgggac gaggggaagc acctgccaga gcgtttgccc
     5521 cgcttccacc aggaaatccg cgaaccggta ggcggacgga ccgagatctc cgattccgaa
     5581 cggcgaggga agcgatgtga cgtgcagcag gatcccgctc tgccgggtcg agtccatgat
     5641 cgatccacgt tggttcagcg ccgtgcggcg gcgccgaaga taacccgcga cggcccgatc
     5701 atcaccctcg acccggccga tttcccggag gctgcacgtg cccgtgtgac cccgcggcgc
     5761 gcgcgcgtgc ggacggcctc ccgtaacaaa gcgccgtggc cgagcgtagc acaaatcgat
     5821 ccccatccgg caggcgaccg cgcatgcagg cctatctcga atcgctcgat ccggcggacc
     5881 tccatttcct cgtcggcctg gtcgagagtc ccgtcacgct cacgaacgat gcgcagctgc
     5941 gatccctcgc cgcccagatg gacgcaggcg atccggacgc acggacgcgc ctcgccgcag
     6001 cgctcgatcg ggagatccga tacctcggaa gctcagacgt ggcctacgcg ttccgctcgc
     6061 tcacgggaag cgaacccggt gtttccgccg gggaaatcct gcgggacgtc tcggggcagc
     6121 tgaaggtgcc ccatccgggc ctcggaacgt tcgaggacca gctggcgcac ctggccgagg
     6181 cgtacgccac gcgggccttc gccgatctcc cggcccacga gcagcagcgc atgctcgaag
     6241 agctgggcgt ggaacgcgac aaggcggccg cagtcctcaa gagatcggcg ggcgacttcg
     6301 cccttccggt gatgatcgag gccttcaacg ttgtcgtcgt gcagggcctc atcaagacga
     6361 tcatcttcgg gaccatcgcg cggatcatcg gccggcagct ggccgcccgg ctcttcgcat
     6421 tcctggccgg acgcgttccc tggtgggtcg gctggatcgg accggccgcc tggaccctgt
     6481 ccgtcggctg gacggcgctg gatctccagg gaccggccgc aaggaagacc gtccccgtca
     6541 tgctctacct ggggctctgc gcggtgcgga aacggaacgt gccggtcagc gcggcgtgag
     6601 gactgagaac tgcccgtaga tctcgtccgc cagccggcca gccggcccac gtcccaggtc
     6661 gatgatgtcc gcgccccgga ccgggctcgc accggccgta tccacataga ccgtgcacag
     6721 gtccaggtgc cgctggaaca gcgaggcgcg cgtctgaagc acctgcaggc ggccgaccgg
     6781 gatgatccac gtgtactgct gcagcacgcc gcggcgcacg accaggtgcc ggtcgtgcag
     6841 gtcgtacccc atgcatcgcc accgcagcac ggccgcgccg agcgccagcg gccacagcga
     6901 cagaagtagc caggcgctcg aatcggtcag gatcactgcc actccggtcg ccgcggcaag
     6961 gcccagcgtg tagcgcacga gcgcgcgccg tatggtcagg cgcgagacgg actcgaggct
     7021 ttgcggaaag gcccagggct ccgtccgcgc gtcgggcatg ccttcccgga tgcgggcgat
     7081 cacggcctgg atctcgccga gcctgccgaa gggaatcgcc accgagtgcc cctgctcccg
     7141 cacgtcggtg cccatcgtct gaacgtcgag cgtgtaccac ccgaaccggc gcatgagagg
     7201 attcgtgcgc acgatcagcg cctggacgcg gtgcagcggg atcgtgccct cccgcacgga
     7261 caggagcccg tgcttgcgat gcagccggtc cttctcgagc gtgaggacga agcgatagaa
     7321 ccgattgaag ttcaccgcga tgccggacac ccagccgacc aggaccgcga ccacgatgcc
     7381 gatggcggcc gccgcccagg gcgacgccgt cgccgctctc gcaagccccg ccaggcgccc
     7441 gcgcgtcaac caggagacca ggacttccgg gtccggctcc acgtactgca ggaaggtgaa
     7501 gatgatcgcg atgtagagca ggctgaatcg gaaggccccc gacagcagca cccgcttcgg
     7561 gtccatcgcc agaagcacct cggagggcgc cgccggcacc ccggtctcct cgtcggcctt
     7621 cggggagctt tccggagccg caacgaccgc ccgctggtag ccccgcacgg tctgccggat
     7681 gcggtgggcc tccgcgaggg aaacgacgtc gagcgccccc tccgcctcct gggtgccggc
     7741 cgtgtagatc cggactcggg cagtccccgt cagccgctgg agcagttgct gctcgatctc
     7801 gatgttctgg atccggtcga cgggaatgtt tctccgccgc ctcgtgagca cgcccgaatg
     7861 gatcacgacc tcgtccggag tcaggcggta ccggaagcgg acatagtgca gtacgagcca
     7921 gggcagcacc accagcgcat acaggaacga gagcacgagg ttgaacagcg ccgacgaatc
     7981 cggagaccgg aagaccggca gcagcaggaa gacgagcgcc ggaatggaaa cagccgcacg
     8041 aaggaccagc gtcatcgggt gaagccgatg ctccggctcc acctgaccgg tgcgctcagg
     8101 ccccccggga tcgccgttct ccggctcggc cggcatgccg gcgccgcggc tcctttcaga
     8161 gtgcatcatc cacgatgtac gccttgatgc ggtcgcggag cctctcggcc tcctcgagtc
     8221 ggagccccgg gaccacgacc tcggaactgc gtgtccccgc cgtgtggacg accagcttcc
     8281 ccagctcgaa ctcccgctcc acgacgtcct gcgagacgtc caggtgctgc acccggcgaa
     8341 gcggaacgat ggtgcgcacc cggttcagga ttccgcgctc cagaacgagc tcctgctcgg
     8401 tcagcgcgaa ccaccagaag cggtaccgga gccgcggcac ccagaagaga atggccgaga
     8461 aagccacgag cacgacgccc ggcactacgc cgagcggcat ccatcgctcc gcatcgaaca
     8521 cgttggcgac gtcgtggatg acggccgcga ggagcgccac ggcccccgct acggccagct
     8581 tgatggccca cacctttcgc acccgcggat ccaggcggtt catccggctg ccccggtccg
     8641 gatccgggat ccccccgtca ggcattctgc accctggata cgagcgccgc aaccgcgcca
     8701 ccggcgaacg gagcgaggag gtagatccac aggtcaccta cgcttccgtc cccaacgagt
     8761 gcactga
//
LOCUS       DBNHQZ010000132         7061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3289064, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000132 DBNHQZ010000000
VERSION     DBNHQZ010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3289064"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16YU_03320"
     CDS             complement(<1..466)
                     /locus_tag="AC16YU_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR6064282.1"
                     /translation="MEWLDVLVLALSFLGLLALGVPISYCIGLSTIATMLVSMQTIPA
                     LTTEAQRIATGLDSFALLAIPFFVLAGQIMSRGGIARRLIDFARAMLGSLPGGLAHIN
                     ITAAMLFGAISGSAAAAATAVGGIMAPEMEREGYESGFGVAVNVTSATTGLLI"
     gene            complement(471..944)
                     /locus_tag="AC16YU_03325"
     CDS             complement(471..944)
                     /locus_tag="AC16YU_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6064283.1"
                     /translation="MRNLKQRIDRVLAWAVILIMGTLVVDVLWQVFTRFVLRDPSSYT
                     EELARFLLIWVGLLGAAYVAGQRRHLAVDLLPGALTGTRRRALDRLIQALAFVFALAL
                     VVGGSRLVWVTLVLGQSSAALGVPLGYVYSVVPLCGLLMAFYALHHFVHPDEEAA"
     gene            complement(947..1930)
                     /locus_tag="AC16YU_03330"
     CDS             complement(947..1930)
                     /locus_tag="AC16YU_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6064284.1"
                     /translation="MRRVRPAALLAAWLVLGLAGCRAHDGVTVVKLGHGLDPGHPVHE
                     AMEHMQARVAELSGGRMRIDLYPSQQLGSERELLELLQLGSLGMTKVAAAVLEGFAPK
                     YKALSAPYVFRDREHRFRVLEGEIGRELLLDSQRYWLRGLTYYDAGSRSFYTKDRPIR
                     APEDLAGLKIRTLESATQVRMVNELGGSATPISWGELYTALQQGIVDGAENNPPSFHT
                     SRHYEVCRYYSLDEHTSIPDVLLVSTIIWDTLSEEERGWLQQAALESSGVQKELWRVA
                     EEEALRIVAEAGVEILRPDKAPFIEKAQPLYEAYRAEPEVYEVIRRIRAVE"
     gene            complement(1927..2955)
                     /locus_tag="AC16YU_03335"
     CDS             complement(1927..2955)
                     /locus_tag="AC16YU_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR6064285.1"
                     /translation="MKVVTFGEIMLRLSTPGFERFVQASRFDVVFGGGEANVAASLAG
                     FGDEAWFVSRVPGHEMGEAALGYLKHAGVRTDAVVRGGDRLGIYFLETGASQRPSKVI
                     YDRAGSAVATMDPDAVDWDGVLEGAAWFHWTGITPALGPLPREGVRRACQAAKRLGVT
                     VSCDLNFRSKLWSPLDAQSVMVPLMEYVDVCIGNEEDAQQCLGLKTRESDVESGVLDG
                     DEYLALARRMRESFGFDVVALSLRESHSATRNGFSAVLCDGRDCPDGVRSRRYDVHVV
                     DRVGAGDAFAGGLIHGLMHLGHRADALEFGVAASVLKHTIPGDFNRVTGDEVNRLVRS
                     GGSGRVER"
     gene            complement(2952..3635)
                     /locus_tag="AC16YU_03340"
     CDS             complement(2952..3635)
                     /locus_tag="AC16YU_03340"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR6064286.1"
                     /translation="MKAMRDRIVERLLDSGAVAVIRMAEASKVVRVAEALAAGGLTAV
                     EITMTVPNALELIRTVSGELGDDVTVGVGTVTDLDTAREAVDAGARFVVGPVTNEDLL
                     RAAAELPVPVVPGAFTPTEVLRAHDAGASFVKIFPADAVGPGFVKALLGPMPSLRLMP
                     TGGIDASNAGAWIRAGAVCVGVGGALLDRQAIADGRYEVVTDRARDLIRAVRSAILET
                     HIHPAAPAS"
     gene            3899..5071
                     /locus_tag="AC16YU_03345"
     CDS             3899..5071
                     /locus_tag="AC16YU_03345"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HIR6064287.1"
                     /translation="MRRASLSTFALVLLLAGCSEQAITSTESEGPNFSAEFRAGGPGP
                     ATPVFLLSPSNVCGMEDAACKHHGGDADGFAGPLFALATAPDGDILVADAGEGIATVD
                     GSTEMVLPAVTGIAPVGRGTAWATAGARVEGGEDDTGQGLYRLAPGRTRKLVNLFDFE
                     ARENPDGDQVASNPFDVASVDGEWAYVTDAAANDLLRIDQDGNVEVVAVFPDELVSTA
                     NLQDLVGCPNPVIPGLAFICGVPMMPAEAVPTSVTIGPDGYLYVGELRGFPAPAGASN
                     IWRVAPGASWAACGSSPDCEKVFDGGFTSIIDLAFGPDGRLYVAELDERSWFAAEVLH
                     AGVGGTVNACDTASGTCEEVATGIPLLTAITFGKDGSLHATRNALIPPLAEVIEIQ"
     gene            complement(5080..6792)
                     /locus_tag="AC16YU_03350"
     CDS             complement(5080..6792)
                     /locus_tag="AC16YU_03350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR6064288.1"
                     /translation="MPRTPTLHRIVRGLLPALLIVGGCGPAPSDDPAPAADEVRFDWF
                     EYTGRDPVFDALPTGTDGYVNPVMAGFYPDPSIVRVGEDYYMVHSTFSYFPGIPVFHS
                     RNLVDWTQIGNVLDRPEQMNLDSLGVSRGVFAPTIRHHEGTFYVVNTLVDAGGNFVVT
                     ATDPAGPWSDPVWLPEVDGIDPSLFFDTDGRAYVTNNGPPAGQPLYEGHRALWIQEFD
                     PATLRTVGPRTMIVDGGVDLSKQPIWIEGPHIFRVDPYYILIAAEGGTGYMHSEVVFR
                     SRELRGPWVPYEGNPILTQRHLDPARHNPVTSTGHADMVRTPDGDWWAVFLGCRPYGP
                     DHYNTGRETFLLPVRWEDGWPVILDVDQAVPMAAERPALPSGAPPAIPHRGNFTVRDD
                     FDGDAPHPYWLQLRTPRETWHRTEAGALVLEARPDSLGGLGQPSFLARRQQHMHATVT
                     TSMRWHPSGEGSKAGIAAFQNRRAWYLLAVAGSASGPRIVLEAGTASGTHRLASAPLA
                     AGSRGPIRLRIDADADRYAFRYALVDSDWVTLADSVDGAVLSTAAAGGFVGAMFGLYA
                     VSGR"
     gene            6864..>7061
                     /locus_tag="AC16YU_03355"
     CDS             6864..>7061
                     /locus_tag="AC16YU_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064289.1"
                     /translation="MPLRSRSRVAYVHGRHPQATAGTAENEIARRASVMIHTLLLVLS
                     GLVVGATLLPLLRSPAWWIRVF"
BASE COUNT         1126 a         2433 c         2459 g         1043 t
ORIGIN      
        1 gaatgaggag gccggtcgtc gcggaggtca cgttcacggc cacgccgaat ccgctctcgt
       61 agccctcccg ctccatctcg ggcgccatga tgccgcccac cgccgtcgcc gcggcggcgg
      121 ccgagcccga gatcgcgccg aagagcatcg ccgccgtgat attgatgtgc gcgaggcctc
      181 ccggaagcga gccgagcatc gcgcgggcga agtcgatcag gcgcctggcg atccctcccc
      241 ggctcatgat ctgccccgcg agcacgaaaa aggggatggc gagcagcgcg aacgagtcca
      301 gtcccgtcgc gatgcgctgc gcctcggtcg taagggcggg gatcgtctgc atcgacacga
      361 gcatcgtcgc gatcgtcgac agcccgatgc agtacgaaat cggcactccc agggccagga
      421 gcccgaggaa gctgagggcg agtacgagga cgtcgagcca ctccatggcg tcaggctgcc
      481 tcctcgtccg ggtgcacgaa atgatgcagc gcatagaagg ccatgagcag gccgcagagc
      541 ggtacgaccg agtagacgta cccgagcggc accccgaggg ccgcggacga ctggccgaga
      601 acgagtgtga cccagaccag gcgcgacccg ccgacgacga gcgcgagcgc gaagacgaat
      661 gccagggcct ggatcaggcg gtcgagcgct cgccgccgtg tgcccgtgag ggctccggga
      721 aggagatcca cggcgagatg cctgcgctgt ccggccacgt aggcggcgcc gagcagcccc
      781 acccagatga gcaggaagcg cgcgagctcc tcggtgtacg agctcggatc gcgcaacacg
      841 aaccgggtga agacctgcca aagcacatcc acgaccaggg tccccatgat gaggatcacg
      901 gcccacgcga gcacccggtc gatccgctgc ttcaggtttc gcatggctat tcgacggccc
      961 ggatcctgcg gatgacctcg tacacctcgg gctcggcccg gtacgcctcg tagagcggct
     1021 gcgccttctc gatgaacggc gccttgtccg ggcgcaggat ctcgaccccg gcctcggcga
     1081 cgatgcgcag ggcctcctcc tcggcgacgc gccagagctc cttctgcacc ccggacgact
     1141 cgagggcggc ctgctgcagc cagccccgct cctcctcgga gagcgtgtcc cagatgatcg
     1201 tactgacgag gagcacgtcg ggaatcgaag tgtgctcgtc gagcgagtag tagcggcaga
     1261 cctcgtagtg gcgcgacgtg tggaaggagg gcgggttgtt ctccgcccca tccacgatgc
     1321 cctgctgcag ggccgtgtac agctcgcccc acgagatggg cgtggcggag cccccgagct
     1381 cgttcaccat gcgcacctgg gtggcgctct cgagcgtgcg gatcttgagc ccggccaggt
     1441 cttccggcgc gcggatcggg cggtccttcg tgtagaagga gcggctgccg gcatcgtaat
     1501 aggtcaggcc gcgcagccag tagcgctggc tgtccaggag gagctcgcgc ccgatctcgc
     1561 cttccagaac ccggaaccgg tgctcccggt cacgaaagac gtagggcgcc gagagcgcct
     1621 tgtacttggg cgcgaagccc tcgaggacgg ccgcggccac cttggtcatc ccgagactgc
     1681 cgagctgcag cagctcgagc agctcgcgct ccgatcccag ctgctggctc gggtagaggt
     1741 cgatccgcat ccggccgccc gagagctcgg ccacgcgggc ctgcatgtgc tccatcgcct
     1801 cgtggaccgg atgcccggga tcgagcccgt gcccgagctt caccaccgtg acgccgtcat
     1861 gggcacggca accggccagt cccagcacga gccacgcggc gaggagcgcc gccgggcgca
     1921 cgcgcctcat cgctccaccc gccccgatcc gccgctgcga accaggcggt tcacctcgtc
     1981 gccggtgacc cggttgaagt cgccgggaat cgtgtgcttc aggaccgacg ccgcaacgcc
     2041 gaactccagc gcgtcggcgc gatgcccgag atgcatcagg ccgtggatga gccccccggc
     2101 gaaggcgtcg cccgccccga cccggtccac gacgtgcacg tcgtaccgcc gggaacgcac
     2161 gccgtcgggg cagtcgcgtc cgtcgcacag cacggcgctg aagccgttac gcgtggcgga
     2221 atggctctcg cgcaggctca gcgccacgac gtcgaatccg aacgattcgc gcatgcgccg
     2281 tgccagcgcc aggtactcgt ccccgtcgag cacgcccgat tccacgtcgg actcccgcgt
     2341 cttcaggccg aggcactgct gggcgtcctc ctcgttgccg atgcagacgt ccacgtactc
     2401 catgagcgga accatgaccg actgcgcgtc aaggggcgac cacagcttcg agcggaagtt
     2461 caggtcgcac gagacggtga cgcccaggcg tttcgccgcc tggcaggcgc gccggactcc
     2521 ctcgcgcggc aggggcccga gcgccggcgt gatacccgtc cagtggaacc aggccgctcc
     2581 ctccagcacg ccgtcccagt cgacggcgtc cgggtccatg gtcgcgacgg ccgagccggc
     2641 gcggtcgtag atcaccttcg agggccgctg gctggccccc gtctccagga agtatatgcc
     2701 cagccggtcg ccaccgcgga cgacggcgtc cgtgcggacg cccgcgtgct tcaggtaccc
     2761 caatgccgcc tcgcccatct cgtgccccgg cacgcgggac acgaaccagg cctcgtcgcc
     2821 gaatccggcc agcgaggccg cgacgttcgc ctctccgccg ccgaacacca cgtcgaaccg
     2881 gctcgcctgc acgaagcgct cgaagcccgg cgtcgagagc cgcagcatga tctcgccgaa
     2941 cgtgacgacc ttcatgaggc gggagcggcg ggatggatgt gggtctcgag gatcgcggag
     3001 cggacggcgc ggatcagatc gcgggcacga tcggtcacga cctcgtaccg cccgtcggcg
     3061 atggcttggc ggtcgaggag cgctccgccc acgcccacgc acacggctcc ggcccggatc
     3121 caggcgccgg cgttcgatgc gtcgatgccg cctgttggca tcaggcgcag ggacggcatg
     3181 gggccgagca gggccttgac gaaacccggg cccacggcgt cggccgggaa gatcttgacg
     3241 aacgacgcgc ccgcatcgtg cgcccgaagg acctcggtcg gggtgaacgc gcccggaacc
     3301 acgggtacgg gcagctcggc ggcggcccgg agcaggtctt cgttcgtgac cggaccgacc
     3361 acgaaacgcg ctccggcgtc caccgcttcg cgggcggtgt ccagatcggt taccgtgccg
     3421 acgcccacgg tcacgtcgtc cccgagctcg ccgctcaccg tccggatgag ctccagcgcg
     3481 ttcggaacgg tcatcgtgat ttcgacggcg gtcagtccgc ccgccgccag cgcctcggcg
     3541 acccggacca ccttggaggc ctccgccatc cggatgacgg ccaccgctcc cgaatccagg
     3601 agccgttcca cgatcctgtc ccgcatcgcc ttcatgatgg gttgccctcg ttgcttcaga
     3661 aggtcgggcc gcgggcaaga tagagcgggc gcggcactga ttccgcgatc gtcggtgtgt
     3721 cgactttctc tccccggatg tattggaacg gtcgcctgcc ggaattacat agcaggtatg
     3781 gatcgggacg acccccgacc ggtccgcgag tgaggccccg tgcttgccgg gagtgaccgg
     3841 tccgctgccc gatccgtgaa cccggatcga ttcacttcaa gacctgagag gtccccccat
     3901 gagacgtgcc tcgctgtcga cgttcgcgct ggtcctgctg ctggccggct gctccgaaca
     3961 ggcgattacc tccaccgaaa gtgaaggacc caacttctcc gcagaattcc gtgcgggcgg
     4021 cccgggtcct gcaacacccg tcttccttct ttccccgtcc aacgtatgcg gcatggagga
     4081 cgccgcctgc aagcaccacg ggggcgatgc tgacggcttc gccggaccgc tttttgccct
     4141 ggccacggct ccagacgggg acattctcgt cgccgatgcc ggtgagggca tcgccaccgt
     4201 ggacggctca acggaaatgg tgctgccggc cgtaacgggc attgctcccg tgggccgggg
     4261 taccgcctgg gccacggcgg gagcccgcgt cgagggcggc gaggacgata cgggccaggg
     4321 gctctatcgc ctggcgcccg gtcgcacgcg aaaactggtc aacctgtttg acttcgaggc
     4381 gagggagaac ccggatgggg accaggtggc ctcgaatccg ttcgacgtgg cgtcagtcga
     4441 cggagagtgg gcctacgtca ccgacgcggc ggccaatgac cttctccgga tcgaccagga
     4501 cggaaacgtg gaggtggtcg cggtctttcc ggacgaactg gtgtccacgg ccaacctgca
     4561 ggacctcgtg ggctgcccga atccggtcat tcccggcctg gcgttcatct gcggcgtccc
     4621 catgatgccc gcggaggccg tgcccacgag cgtcaccatc ggcccggacg gctacctgta
     4681 cgtgggtgag ctcaggggat ttcccgcgcc ggcaggcgca tcgaacatct ggcgcgtggc
     4741 tccgggtgca tcgtgggcgg cgtgcggctc gagcccggac tgcgagaagg tgttcgacgg
     4801 aggattcacg tccatcatcg atctggcctt cggcccggac ggccgcctgt acgtggccga
     4861 gctcgacgaa cggagctggt tcgcggccga ggtgcttcac gcgggcgtgg gcggcacggt
     4921 caacgcgtgt gacaccgcct ccggcacgtg cgaggaggtc gctaccggca ttccgctcct
     4981 gaccgcgatt acgttcggaa aggacggatc gctccatgcg acgaggaatg ccctgatacc
     5041 gcccctggcc gaggtgatcg agatccagta ggggggactc tacctgcccg atacggcgta
     5101 gagtccgaac atcgccccga cgaagccgcc ggccgcggcg gtcgagagga ccgcgccgtc
     5161 gacggagtcg gcgagggtga cccagtcgga gtccacgaga gcgtaccgga acgcgtaccg
     5221 gtcggcgtcc gcgtcgatgc gcagccggat cggcccacgg gagccggcgg ccagcggggc
     5281 ggacgccagc cggtgcgttc ccgaggccgt gcccgcttcc agcacgatcc gcggtcccga
     5341 ggcggacccc gcgacggcca ggaggtacca cgcccgcctg ttctggaagg cggcgatccc
     5401 cgccttcgag ccttcgcccg acgggtgcca acgcatggac gtggtcaccg tcgcgtgcat
     5461 gtgctgctgg cggcgcgcga ggaaggaggg ctggcccagg ccgccgagcg aatcggggcg
     5521 cgcctccagg acgagcgccc cggcctccgt gcggtgccag gtctcgcgcg gtgtccggag
     5581 ctgaagccag taggggtggg gcgcgtcgcc gtcgaagtcg tcgcggacgg tgaagttgcc
     5641 gcggtgaggg atcgcgggcg gggcgccgga ggggagggcg ggtcgctcgg cggccatcgg
     5701 cacggcctgg tcgacgtcga gaatcacggg ccagccgtcc tcccagcgga ccgggagcag
     5761 gaaggtctcg cggccggtat tgtagtggtc cggcccgtaa ggccggcacc cgaggaagac
     5821 cgcccaccag tcgccgtccg gcgtacggac catgtccgca tgccccgtgg acgtcacggg
     5881 gttatgcctg gccggatcca ggtgtcgttg ggtcaggatg gggttgccct cgtacgggac
     5941 ccacgggccc cggagctcgc ggctcctgaa cacgacttcg gagtgcatgt acccggtccc
     6001 gccctcggcg gcgatcagga tgtagtacgg gtccacgcgg aagatgtgtg gcccctcgat
     6061 ccagataggc tgcttcgaaa ggtcgacgcc cccgtcgacg atcatggtcc gtgggcccac
     6121 ggtccggagc gtggccgggt cgaactcctg gatccacagc gcgcggtgcc cttcgtagag
     6181 gggctgtcca gcgggcggac cgttgttcgt cacgtacgcg cggccgtccg tgtcgaagaa
     6241 gagcgacgga tcgatcccgt ccacctccgg cagccagacg ggatccgacc agggacctgc
     6301 cgggtcggtc gccgtcacga cgaagttgcc gccggcatcc acgagcgtgt tcaccacgta
     6361 gaacgtgccc tcatggtgcc ggatggtcgg ggcgaagacg ccgcgcgaaa cgcccagcga
     6421 gtccaggttc atctgctccg ggcggtccag cacgttgccg atctgggtcc agtctacgag
     6481 gttccggctg tggaagaccg ggatgcccgg gaagtacgaa aacgtcgagt ggaccatgta
     6541 gtagtcctcg cccacccgaa cgatgctcgg atccgggtag aaacccgcca tgaccgggtt
     6601 cacgtatccg tccgtgccgg tagggagtgc gtcgaagacc gggtcacggc cggtgtactc
     6661 gaaccagtcg aagcgcacct cgtcggccgc aggtgcggga tcatcggacg gcgcgggccc
     6721 gcaacccccg acgatcagca gggccgggag caggccgcgg acgattcggt ggagcgtcgg
     6781 ggtgcggggc atggcgcgac agagtcgggt ggtccggacg atgcatgata ccgcgcgtgg
     6841 cgcacaggat cgaagcacct cgcttgccac tccgcagtcg atcccgcgtg gcgtatgttc
     6901 acggacgcca tccgcaggcc acggccggga ccgcggagaa cgagatcgca cgtcgcgcgt
     6961 cggtgatgat ccatacgctc ctcctggttc tctcagggct cgtcgtggga gcgacgctgc
     7021 tccccctcct gcggtcccct gcgtggtgga tccgcgtgtt c
//
LOCUS       DBNHQZ010000133         7898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_37273, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000133 DBNHQZ010000000
VERSION     DBNHQZ010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_37273"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..962
                     /locus_tag="AC16YU_03360"
     CDS             150..962
                     /locus_tag="AC16YU_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064290.1"
                     /translation="MQQDLLELERLIDRGETAVAMPILERAVEMEPGYAAARLLLARA
                     YEGEGRMGDAVSEWRVVRALVPDSPTAVTGLKRALRLRMLWAADPAVWATDDGDAAAA
                     AAPPTDVAEDPAAEPFVAEGPAAEPEEMPPPEPEEPPPAAFEAPMPAEPGAGEPEQAL
                     PAEPEPLRAAEPAEPDVPDWDDERPQNLDELIRELETARIVPDPTIPPAVAEDLEPDV
                     DDVVSETLARIYENQRHYGEAARVYEKLALQQPGRAPEFRQKAADLRTRAGT"
     gene            970..2160
                     /locus_tag="AC16YU_03365"
     CDS             970..2160
                     /locus_tag="AC16YU_03365"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR6064291.1"
                     /translation="MTAEPTGQPGEDRPRLVAGVMSGTSVDGVDVALVRLRGSGTALE
                     WSVEAHHSLPFRADLRERLLAMGEADVVRLDDLALMHVRLAHAYADAIRDALRAADGR
                     ARDLDLVGCHGQTVRHLPEPRAFCGTDVRATLQLGSGPTLANLVGVPVVTDFRAADMA
                     LGGEGAPLVPYVDLVLLGDAAEHRAALNIGGIANVTALPAGRSADGVVAFDTGPGNMV
                     SDALCRMHLGIPFDRSGDVARSGTPDEALLGELLAEVYFTRRPPKSTGREAFGEAYVA
                     DLVRRAGARGLSAADQVATAAALTADSVTGALRAFVPVAVSRVLVSGGGVHNLAIMER
                     MASALPRVRFESTAAVGLDPDAKEAVAFAVLAHETASGVPAGMPSVTGARKAAILGSV
                     SHPS"
     gene            2185..3234
                     /gene="thiL"
                     /locus_tag="AC16YU_03370"
     CDS             2185..3234
                     /gene="thiL"
                     /locus_tag="AC16YU_03370"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR6064292.1"
                     /translation="MSEQFTPVSHVGEFGLIDRMRDRLGETAPGGDLLCTIGDDAAVY
                     RVSDTEAHVITTDALIEGVHFERAFMPWGHLGRKAVAVNVSDVAAMNAVARYAVVTVG
                     IPRNVSVQMIDALYEGLRAGCETYGLRLVGGDTTAAPLLALSVTVVGEAPIDRIVYRR
                     GARPGDLLCVTGDVGAAYAGLQVLLEQRTAMRELGDAYVPDLDPYRTVIGRQLVPTAR
                     TDVVADWAARGVRPRALIDVSDGVASEVHHVCRASGCGAVVRAAALPVDLETRAVADE
                     RLEDVDTFALFGGEDYELLFALDPEDLDRLDPAQLSVIGQFTERDAGIQLQTAEGDVI
                     PLDASGFSHFGEDAG"
     gene            complement(3290..5824)
                     /locus_tag="AC16YU_03375"
     CDS             complement(3290..5824)
                     /locus_tag="AC16YU_03375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR6064293.1"
                     /translation="MRTLLLFSLLAIAASSTALAQDPLPSIEEKTDGLERRDGYFPLY
                     WDEDAGQVWLEVPRVGEDFLYAVSLAAGLGSNDIGLDRNQLGPQRLVRFERVGRRLLL
                     RAPNLRFRASTDSEAERRSVEDAFAGSVVWGFEVAARTGDRFLVDATPFVVRDAHGVI
                     PRLAGSRQGTFRLDASRSAPSPDVLKAFPRNTEMEATLTFTSDDPGRWVRDAAADPGA
                     VTLRVRHSFVRLPDPGYEPRAFDPRSGYFELSYADYAAPTGEDMTVRLISRHRLEKAH
                     PDSAVSAPVEPIVYYLDPGTPEPVRSALLDGARWWEEAFLAAGFRDAYRVEVLPEGAD
                     PLDARYNVINWVHRATRGWSYGSSLVDPRTGEIIKGHVLLGSLRIRQDYLLMEGLLAP
                     YDAPVGGAPDPDPMLEAALARIRQLSAHEVGHTLGLSHNYAASIDDRASVMDYPAPLV
                     VLGADGRPDAGWAYATGVGAWDVVAIRYGYAQFPGGADEQAGLAAILDEARGRGLHYL
                     TDQDARPTGSAHPLAHLWDNGTDATQGLRDMMAVRRAALERFGAAALRGGRPLAQLHE
                     ALVPLYLHHRYQVEAASKLVGGVRYDYQLRGAPGDAPEPVPAREQEDALSTLLGALDP
                     RELALPPPIRTSIPPRPPGFDATRELFAGYADPVFDAYAPAEVAAAMVVDLLLSSERM
                     ARLLYQADQGGGLPSLSDVLAQVSESLWQADVPRDPYEAELQRIVQQVWVDGLIAAAV
                     RPGIAPGVRARLRQHLVDLLDYVGATPGEDYETIAHRATVFDQLDRFLVRLYDPAERT
                     VRSETPPGSPIGAGAGSGEVETGRVRLRQAWLGAWQPDDGYCSMGG"
     gene            6060..7559
                     /gene="atpD"
                     /locus_tag="AC16YU_03380"
     CDS             6060..7559
                     /gene="atpD"
                     /locus_tag="AC16YU_03380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6064294.1"
                     /translation="MEAKALASGEVVQIVGPVIDAQFPADGVPDILDALTIDREDGST
                     LVLEVQQHLGENRVRTIAMDSTDGLTRGTTITNTGQPIAMPVGPEIRGRLFNVVGRAI
                     DGLPQPKAEGYRPIHAEPPEFDELATSVEMLETGIKVIDLLEPYARGGKIGLFGGAGV
                     GKTVIIMELINNIAKAHEGLSVFAGVGERTREGNDLLREMLESGVVDYGKEFLESMEK
                     GGWDLDKVDMNAIGNSNISLVFGQMNEPPGARARVALSGLAVAEYFRDLGGRDVLLFI
                     DNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGELQERITSTRKGSITSVQAIYVP
                     ADDLTDPAPATTFAHLDATTVLSRQISSLGIYPAIDPLDSTSRILDPRVLGDEHYNTA
                     QAVKELLQRYKELQDIIAILGMDELSDDDKQVVARARRAQRYMSQPFFVAQQFTGFEG
                     KYVKIEDTIRGFRMILDGELDHLPERAFAYKGVIEEVIADGEKMLAAEA"
     gene            7574..>7898
                     /gene="atpC"
                     /locus_tag="AC16YU_03385"
     CDS             7574..>7898
                     /gene="atpC"
                     /locus_tag="AC16YU_03385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR6064295.1"
                     /translation="MADSLHVEIVSPSGRVFHGDARGVVAPGTEGSFAVLKNHAPMIA
                     SFEVGSIDVTLLTGDRIRFATSGGFLEVLNNTVTILAETAELASDIDVERARESERKA
                     LERLAE"
BASE COUNT         1143 a         2803 c         2895 g         1057 t
ORIGIN      
        1 cgggagcggc gcgatcggtc ggccgttcgg gcggtgccgg aagtggcccg gcgggccccg
       61 atggcggcaa tccgtcccgg gaagagcgtg gcattggcgg gagcacctcg aatggctact
      121 ttccccctgc ctcaaggctc ccctcgaaga tgcagcagga cctcctggaa ctcgaacggc
      181 tcatcgaccg cggcgagacc gccgtcgcca tgccgatcct ggagcgggcc gtggagatgg
      241 agccgggata cgcggcggcg cgcctgctgc tggcccgcgc atacgagggt gagggccgga
      301 tgggcgacgc cgtttccgag tggcgcgtgg tccgggccct ggtccccgac agccccaccg
      361 cggtcaccgg cctgaagcgg gcactgcggc tccggatgct gtgggcagcg gaccccgccg
      421 tgtgggcgac ggacgatggg gacgccgccg ccgcggccgc gccgccgacc gacgtcgcgg
      481 aggatccggc agcggagcct ttcgtcgcgg aggggccggc cgcggagccg gaggaaatgc
      541 cgccgcccga gccggaggaa ccgccgcccg cggcattcga agccccgatg ccggcagagc
      601 cgggcgccgg cgagccggag caagcgctgc cggccgagcc ggaaccgctg cgcgccgcgg
      661 aacccgccga gcccgacgtc cccgactggg acgacgagag gccgcagaac ctggacgagc
      721 tgatccgcga gctcgagacg gcgcgcatcg tgccggaccc gaccatcccg cccgcggtgg
      781 ccgaggacct ggagcccgat gtggacgacg tggtgtccga gaccctcgcc cgcatctacg
      841 aaaaccagcg ccactatggc gaggcggccc gggtgtacga gaagctcgcg ctccagcagc
      901 cgggacgcgc tccggaattc aggcagaagg ccgccgacct caggacgcgg gcggggacgt
      961 agcgcgcaca tgaccgccga gccgacagga caaccgggtg aggaccggcc caggctcgtg
     1021 gcgggcgtga tgtccgggac gtcggtcgac ggcgtggacg tggcgctcgt gcggctgcgc
     1081 gggtcgggca cggcgctcga atggagcgtc gaggcccacc actcgctccc gttccgggcg
     1141 gacctgcgcg agcggctcct ggcgatgggc gaggcggacg tcgtccgcct ggacgatctc
     1201 gccctgatgc acgtccggct ggcccacgcg tatgccgacg ccatccgcga cgcgctgcgg
     1261 gccgccgacg gcagggcccg cgacctggac ctcgtcggat gccacgggca gacggtgcgg
     1321 catcttcccg agccccgtgc cttctgcgga acggacgtcc gggccacgct gcagctcggg
     1381 agcggaccca cgctcgcgaa cctggtcggc gtgccggtgg tcaccgactt ccgggcggcg
     1441 gacatggcgc tgggagggga gggcgcgccg ctcgttccgt acgtggacct cgtgctcctg
     1501 ggcgacgccg cggagcaccg cgcggcgctc aacatcggtg gaatcgccaa cgtgacggcg
     1561 ctgccggcgg gccggtcggc cgacggcgtc gtcgcgttcg acacgggtcc cggcaacatg
     1621 gtgagcgatg cgctgtgccg gatgcacctg ggcattccgt tcgaccgtag cggcgacgtt
     1681 gcccgttccg ggacgcccga cgaggccctg ctcggcgagc tgctggccga ggtgtatttc
     1741 accaggcgcc cgcccaagtc caccggccgc gaggctttcg gcgaggccta cgtggcggac
     1801 ctggtccgcc gggctggggc cagggggctt tcggcggccg accaggttgc cacggccgcg
     1861 gcgctgaccg cggactcggt gacgggcgcc ctgcgcgcct tcgtgccggt cgcggtctcg
     1921 cgcgtgctcg tctcgggcgg cggcgtccac aacctggcca tcatggagcg catggcgtcg
     1981 gcgcttccgc gcgtgcggtt cgagagcacc gcggcggtcg gcctggatcc cgacgcgaag
     2041 gaggccgtcg cgttcgcggt gctggcacac gagacggcct ccggcgttcc tgcgggcatg
     2101 ccgtcggtga ccggcgcccg gaaggccgcg atcctcggct ccgtatcgca cccgtcctga
     2161 ccgcacccga tcacgggtcg aaccatgtcc gaacaattca ctcccgtctc ccacgtgggc
     2221 gagttcggcc tcatcgaccg gatgagggac cgtctcggcg agaccgcccc agggggagac
     2281 ctgctctgca ccatcggcga cgatgcggcc gtgtaccggg tctcggacac ggaggcgcac
     2341 gtgatcacga ccgatgccct gatcgagggc gtccacttcg agcgcgcatt catgccctgg
     2401 gggcacctgg gccgcaaggc ggtggccgtg aacgtgagcg acgtggccgc catgaacgcc
     2461 gtggcccggt atgccgtggt gacggtgggc attccccgca acgtgtcggt ccagatgatc
     2521 gatgcgctct acgaggggct ccgcgcgggc tgcgagacgt acggcctccg gctggtcggc
     2581 ggcgacacga cggcggcgcc cctgctcgcg ctctcggtca cggtcgtggg cgaagccccc
     2641 atcgaccgga tcgtgtaccg tcggggcgcg cggcccggcg acctcctgtg cgtgacgggc
     2701 gacgtggggg ccgcctacgc tggcctccag gtgctgctcg agcagcggac ggccatgcgc
     2761 gagctgggcg acgcgtacgt gccggacctg gatccctacc ggacggtgat cggccggcag
     2821 ctggtcccga ccgcccgcac ggacgtcgtt gcggactggg cggcccgggg cgtacgaccc
     2881 cgcgcgctca tcgacgtgtc cgacggcgtg gcctccgaag tgcaccacgt gtgccgggcg
     2941 agcggatgcg gggcggtggt ccgcgcggca gcgctgcccg tcgacctcga gacccgcgcc
     3001 gtggcggacg agcggctgga ggatgtcgat acgttcgcgc tcttcggggg cgaggattac
     3061 gagctcctct tcgcgctcga cccggaggat ctggaccgcc tggatcccgc ccagctcagt
     3121 gtcatcgggc agttcaccga gcgggacgcg ggcatccagc tgcagacggc cgagggggac
     3181 gtgatcccgc tcgacgcctc gggcttctcg cacttcgggg aagacgcggg ttgagcccgg
     3241 gcgcgccgcc tcggccgccc gtcgagcagc gtcgcccggc cgcctcgcct cagccgccca
     3301 tcgagcagta gccgtcgtcc ggctgccagg ctccgagcca cgcctgccgc aggcgcacgc
     3361 ggcccgtctc gacttcgccc gagcccgcac ccgcgccgat cggcgacccc ggcggcgttt
     3421 cgctgcgtac ggtgcgctcc gccggatcgt acagccggac caggaaccgg tccaactggt
     3481 cgaagacggt cgcccggtgc gcgatcgtct cgtagtcctc gcccggtgtg gcgccgacgt
     3541 agtccaggag gtcgaccagg tgctggcgca ggcgcgcccg cacgcccggc gcgatcccgg
     3601 ggcgcacggc ggccgcgatc aggccgtcga cccacacctg ctgcacgatg cgctggagct
     3661 cggcctcgta ggggtcgcgc ggcacgtcgg cctgccacag ggactccgac acctgggcca
     3721 gcacgtcgct cagcgagggc aggccgccgc cctggtccgc ctggtacagg aggcgcgcca
     3781 tgcgctcgga gctcaggagc aggtcgacga ccatcgcggc cgcgacctcg gccggcgcgt
     3841 aggcatcgaa gacggggtcg gcgtagccgg cgaagagctc ccgcgtggcg tcgaagccgg
     3901 gtgggcgggg cgggatggac gttctgatag gcggcggcag ggccagctcg cggggatcga
     3961 gcgcgccgag gagcgtcgag agggcgtcct cctgctcccg cgccggcacg ggctcgggcg
     4021 catcgcccgg ggcgcctcgc agctggtagt cgtagcgcac gccgcccacg agcttcgacg
     4081 ccgcctcgac ctggtaccgg tggtgcaggt acagcggcac gagggcctcg tgcagctggg
     4141 ccagcggccg tccaccgcgc agcgcggcgg ccccgaaccg ctccagggcc gcccggcgca
     4201 cggccatcat atcccggagc ccctgcgtcg cgtcggtgcc gttgtcccac aggtgggcga
     4261 gcggatgggc cgatcccgtg ggccgtgcgt cctggtccgt gaggtagtgc aggccgcggc
     4321 cacgcgcctc gtccaggatg gccgcgagcc ctgcctgctc gtccgcgccg cccgggaact
     4381 gcgcgtaccc gtaccggatc gcgaccacgt cccacgcgcc gacgcctgtc gcgtaggccc
     4441 accccgcgtc gggcctcccg tcggcaccca ggaccaccag gggcgccggg tagtccatga
     4501 cggacgcccg gtcgtcgatg gaggccgcgt agttgtgcga gaggcccaac gtgtgtccga
     4561 cctcgtgggc cgagagctgc cggatgcggg cgagcgcggc ctcgagcatc gggtccgggt
     4621 cgggcgcgcc gccgaccggc gcgtcgtacg gcgcgaggag gccctccatc agcaggtagt
     4681 cctgccggat ccgcagcgat ccgagcagca cgtgcccctt gatgatctcg cctgtcctcg
     4741 gatcgaccag gctcgagccg taactccagc cgcgggtggc ccggtgcacc cagttgatca
     4801 cgttgtagcg ggcgtcgagc gggtcggcgc cctcgggcag cacctcgacc cggtaggcgt
     4861 cccggaagcc ggccgcgagg aaagcctcct cccaccagcg cgcgccgtcc agcagcgcgg
     4921 agcgcacggg ctcgggcgtg cccgggtcca ggtaatagac gatgggctcg accggcgccg
     4981 aaaccgcgga atcgggatga gccttctcga gccggtgccg cgagatgagc cggacggtca
     5041 tgtcctcgcc ggtcggggcg gcgtagtcgg cgtacgaaag ctcgaagtag ccggaccgcg
     5101 gatcgaaggc gcggggctcg taaccgggat cgggcaggcg cacgaaggag tggcgcacgc
     5161 gcagcgtgac ggctcccggg tcggccgcgg cgtcacgcac ccaccggccg gggtcgtcgg
     5221 acgtgaaggt cagcgtggcc tccatctcgg tgttgcgcgg gaaggccttc aatacgtccg
     5281 gactcggggc cgagcgcgag gcgtcgagcc ggaacgtgcc ctgccgcgac ccggcgaggc
     5341 gcgggatcac gccgtgtgcg tcgcggacca cgaagggcgt ggcgtccacg aggaaccggt
     5401 ctcccgtgcg ggcggcgacc tcgaagcccc agaccacgct cccggcgaag gcgtcctcga
     5461 ccgagcgccg ctccgcctcg ctgtcggtcg aggcccggaa ccggaggttg ggggcccgca
     5521 gcaggagccg ccggccgacc cgctcgaagc ggaccaggcg ctgcggcccg agctggttgc
     5581 ggtccagccc gatgtcgttc gacccgaggc ccgccgcgag ggacacggcg tacaggaagt
     5641 cctcgccgac tcgcggtact tcgagccaga cctggcccgc gtcctcgtcc cagtacagcg
     5701 ggaagtaccc gtcgcgccgc tccaggccgt cggtcttctc ctcgatcgac gggagcggat
     5761 cctgggccag ggccgtcgac gatgcggcga tggcgagaag ggagaagagc aggagcgtgc
     5821 gcatgggacc ggtggctgaa tcgacgcggg aaggtacgtg aacaggctcg caggcgcccg
     5881 gatcgggggc cggcgaatcc ccggtatctt aacgatcccc gccgccatgc gtccgttgga
     5941 gcgtcggtat tccgcaaccg gcggccgtaa tttcgacgcc cttcgtccgc gcggccggac
     6001 ccccgccggt ccgcgatcga gccgaaggga ttcaaaccgc ctcagaacgc acagcattga
     6061 tggaagcgaa agctcttgcc tcgggcgaag tcgtccagat cgtcgggccc gtcatcgacg
     6121 cccagttccc cgcggacggg gttcccgaca tcctggacgc gctcacgatc gaccgcgagg
     6181 acggatcgac cctggtgctc gaggtgcaac agcacctcgg cgagaaccgg gtccggacca
     6241 tcgcgatgga ctcgaccgac ggcctgaccc gcggcacgac catcacgaac accggccagc
     6301 ccatcgccat gccggtcggc cccgagatcc gcggccggct cttcaacgtg gtgggccgcg
     6361 ccatcgacgg ccttccgcag ccgaaggccg agggctatcg cccgattcat gcggagcccc
     6421 cggagttcga cgagctcgcc acctcggtcg agatgctcga gacgggcatc aaggtcatcg
     6481 acctgctcga gccgtacgcg cggggcggga agatcggcct cttcggcggc gccggcgtcg
     6541 gcaagacggt catcatcatg gagctcatca acaacatcgc gaaggcgcac gaggggctct
     6601 cggtcttcgc cggtgtgggt gagcgcacgc gcgaggggaa cgacctgctc cgcgagatgc
     6661 tggagtcggg cgtcgtcgac tacgggaagg aattcctgga gtctatggag aaagggggat
     6721 gggacctgga caaggtcgac atgaacgcca tcggcaactc gaacatctcg ctcgtcttcg
     6781 ggcagatgaa cgagcccccg ggagcccgcg cccgcgtcgc cctttcgggc ctcgcggtgg
     6841 cggagtactt ccgcgacctg ggcgggcgcg acgtgctcct cttcatcgac aacatcttcc
     6901 gcttcacgca ggccggctcc gaggtgtcgg ccctgctcgg ccgcatgccg agcgccgtgg
     6961 gctaccagcc gacgctcgcc accgagatgg gcgagctgca ggagcgcatc acgtccaccc
     7021 ggaagggctc gatcacgtcg gtgcaggcca tctacgtgcc ggccgacgac ctgaccgacc
     7081 ccgcgccggc gacgacgttc gcgcacctcg acgcgacgac ggtgctctcg cggcagatct
     7141 cgtcgctggg catctacccg gccatcgacc cgctcgactc gaccagccgc atcctggacc
     7201 cgcgcgtcct gggcgacgag cactacaaca cggcccaggc cgtgaaggag ctcctgcagc
     7261 ggtacaagga gctgcaggac atcatcgcga tcctgggcat ggacgagctc tccgacgacg
     7321 acaagcaggt ggtcgcccgc gcccgccgcg cgcagcgcta catgagccag ccgttcttcg
     7381 tggcgcagca gttcaccggc ttcgagggca agtacgtcaa gatcgaggac accatccggg
     7441 gcttccgcat gatcctcgac ggcgagctgg accaccttcc cgagcgcgcc ttcgcctaca
     7501 agggcgtgat cgaggaggtc atcgccgacg gcgagaagat gctcgcggcc gaggcctgat
     7561 cccgcacact gccatggccg actccctgca cgtcgagatc gtttcgccgt cgggccgcgt
     7621 gttccacggc gacgccaggg gcgtcgtcgc gcccggcacc gagggctcgt tcgcggtgct
     7681 gaagaaccac gcgccgatga tcgcctcgtt cgaggtcggc tcgatcgacg tgacgctgct
     7741 gacgggcgac cgcatccggt tcgcgacgtc gggcggcttc ctcgaggtac tgaacaacac
     7801 ggtcacgatc ctcgccgaga cggccgagct cgcctccgac atcgacgtgg agcgggcccg
     7861 tgagtccgag cggaaggcgc tcgagcggct ggccgagg
//
LOCUS       DBNHQZ010000134         3725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1392738, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000134 DBNHQZ010000000
VERSION     DBNHQZ010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1392738"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC16YU_03390"
     CDS             complement(<1..192)
                     /locus_tag="AC16YU_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064296.1"
                     /translation="MDLPDTIATRRLRVDTDTLDRAQWALFPEERSRMRTFRSEERRD
                     SFAVGRLAARELIAARLGCP"
     gene            complement(245..859)
                     /locus_tag="AC16YU_03395"
     CDS             complement(245..859)
                     /locus_tag="AC16YU_03395"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR6064297.1"
                     /translation="MAHTLPDLPYAHDALEPHVDTLTMQIHHGKHHQGYVNNLNAALG
                     DHPALAELPLRELLGGIESVPEAIRGAVRNNGGGHANHSLFWTIMSPRGGGAPAGELA
                     DAIDATFGSFDGFKGAFSKAAATRFGSGWAWLVVDASGKLQCYSTANQDSPFMDGHTP
                     ILGLDVWEHAYYLQYQNRRPDYIAAFWNVVDWDAVGANFEAAKG"
     gene            complement(998..1588)
                     /locus_tag="AC16YU_03400"
     CDS             complement(998..1588)
                     /locus_tag="AC16YU_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064298.1"
                     /translation="MRDLIRELIPHAPEHGLYVAPDVPPDRLRNAVRDYAPDIDEGDV
                     VALYDATILRSAKDGALFTADRFVFQNHDLEAPQTVRYADIVAVRSDRKLLGGRKVIL
                     EVNRGRATIELVIDFSGRPGAAGPVARFLSEAMLLESDEERRRAGGERPASEESGGDT
                     DRAAVRAALDRLLFEGRLSPSDRDRLLDALGPTDEA"
     gene            complement(1635..3122)
                     /locus_tag="AC16YU_03405"
     CDS             complement(1635..3122)
                     /locus_tag="AC16YU_03405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR6064299.1"
                     /translation="MDLSTAYDSLIGDLFAAEALVLVALVGLVLVVVDAFRNDHPSLP
                     WLGLLAVVAGLVLEVQKIGDPTGTAFYGMIRTGGYAAWLNTVILLSAGLTILLSPSYL
                     GRMKHNYGEVYAIILFASLGMMTLAAANSLVTVFVGLETMSVCLYIITGLVRDDEGAI
                     ESALKYFLLGAFATGFFLYGIALLYGATGTFYLPEIAEYLALNGTSTLFWAGTALLMV
                     GFLFKVSAVPFHMWTPDVYQGAPTTLTGFMATASKAAAFGSLILVLYLALPAERWTTV
                     LAVIAVITMVYGNIVALVQSNVKRMLAYSSIAHAGYILVALAAGTAAGYSGALFYLLV
                     YAVMNIGAFGVMALLEWDGLGGREQTLDSLAGVGHRRPILGWTMVFFMFSLAGFPPLG
                     GFLGKYAVFAPAVDAGLTWLVIVGVLASAASAYYYLRVLVVFFMKTADEVGVPVSGGA
                     FPLPRGSAAVLIACAAALVALGVAPQLLDVTASLFSSPGSFTALP"
     gene            complement(3156..3452)
                     /locus_tag="AC16YU_03410"
     CDS             complement(3156..3452)
                     /locus_tag="AC16YU_03410"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6064300.1"
                     /translation="MTDRTPLEPDALDRTLASLPGWGSEGDRLIKTFTFGSFREAMSF
                     VVRLAFEAEDMDHHPELTNVYNQVTVALTTHSAGDRITAADAELARRIEAISWV"
     gene            complement(3466..>3725)
                     /locus_tag="AC16YU_03415"
     CDS             complement(3466..>3725)
                     /locus_tag="AC16YU_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR6064301.1"
                     /translation="VRDFALPEMAHPDSWFFLQAISVAAVFFGAMSYIGNAPNFMVKA
                     IAEANGVETPSFMGYIVRYSIPILLPVYFVIFLVFYSGWVL"
BASE COUNT          643 a         1216 c         1338 g          528 t
ORIGIN      
        1 gggacagccc aggcgcgcgg cgatcagctc gcgcgcggcc aggcggccca cggcgaagct
       61 gtcccgccgc tcttcgctcc gaaaggtgcg catcctcgag cgctcctccg ggaagagggc
      121 ccactgcgcg cggtcgagcg tgtccgtatc cacccggagg cggcgcgtgg ctatggtgtc
      181 cgggagatcc atcgatcggc agccttccgg aggcgggcac gcgccgggcg cgcgcgccga
      241 tcggtcaacc cttcgcggcc tcgaaattcg cgccgacggc gtcccagtcg acgacgttcc
      301 agaacgcggc gatgtagtcc gggcggcggt tctggtactg caggtaatac gcgtgctccc
      361 agacgtccag gcccaggatc ggcgtgtggc cgtccatgaa cgggctgtcc tggttggcgg
      421 tcgagtagca ctgcagcttg cccgaggcgt ccacgacgag ccacgcccag ccgctgccga
      481 agcgcgtggc cgcggctttc gagaaggccc ccttgaaacc gtcgaaggag ccgaacgtgg
      541 cgtcgatcgc gtcggcgagc tcgccggccg gggcaccgcc tcctcgtggg ctcatgatcg
      601 tccagaacag cgagtggttg gcatgcccgc cgccgttgtt tcgcaccgcg ccgcggatcg
      661 cctccgggac ggactcgatg ccgccgagga gctcacgcag gggcagttcc gcgagcgccg
      721 ggtggtctcc gagcgccgcg ttcaggttgt tcacgtagcc ctggtgatgc ttgccgtggt
      781 ggatctgcat cgtgagggtg tcgacgtgcg gctcgagcgc gtcgtgggcg tacgggagat
      841 cgggaagggt gtgggccatg ggggaacgtg aatcggttgg ttccgaaatc gagtcgaagc
      901 gtgacgacct ttcgtactgc cgcggggccg gatggttcgg cgcacgggtc gctccggcgg
      961 gcccgaaaga cgccgccacg gtcgacacgg gcactcccta cgcctcgtcc gtcggcccca
     1021 gcgcgtcgag caggcggtcc cggtccgagg gcgaaagccg gccctcgaag aggagccggt
     1081 cgagggccgc acggacggca gcgcggtccg tgtcgccgcc ggattcctcc gacgccggcc
     1141 gctcgccgcc cgcacggcgg cgctcctcgt cggactcgag cagcatcgcc tccgacagga
     1201 agcgcgccac gggcccggcg gcgcccggcc ggcccgagaa gtcgatgacg agctcgatcg
     1261 tcgcgcggcc gcggttcact tccaggatca ccttccggcc tccgagcagt ttccggtcgg
     1321 agcggaccgc gacgatgtcg gcgtagcgga cggtctgcgg agcctcgaga tcgtggttct
     1381 ggaacacgaa ccggtccgcc gtgaagaggg cgccgtcctt ggcgctccgg aggatcgtgg
     1441 cgtcgtagag cgcgacgacg tccccttcgt cgatgtccgg cgcgtagtcg cgcacggcgt
     1501 tgcgcagccg atccggcggc acgtccggcg cgacgtacag cccgtgttcg ggggcgtgcg
     1561 ggatgagctc gcggatcagg tcacgcatgg gcgatggttc cgtacgaagg tcgcggcgcc
     1621 cgcgtccagc ggtcctaggg cagggccgtg aacgacccgg gcgacgagaa gaggctcgcc
     1681 gtgacgtcca gcagctgcgg cgcgacgccg agcgccacga gggccgccgc gcaggcgatc
     1741 agcacggccg ccgaaccgcg gggcaggggg aaggcaccgc cggaaaccgg cacgcccacc
     1801 tcgtccgccg tcttcatgaa gaagaccacg agcacgcgca ggtagtagta cgcggacgcc
     1861 gcgctcgcca ggacgcccac gatcacgagc caggtgaggc ccgcgtcgac ggccggggcg
     1921 aagaccgcgt acttgcccag gaagccgccc agcggcggga atcccgcgag actgaacatg
     1981 aagaagacca tggtccagcc gagaatcggg cggcggtgcc cgacgccggc cagcgaatcc
     2041 agcgtctgtt cacggccgcc gaggccgtcc cattccagga gcgccatgac gccgaaggcg
     2101 ccgatgttca tgacggcata gaccaggagg tagaacagcg cgcccgaata gccggccgcg
     2161 gtgccggccg cgagcgcgac caggatgtac ccggcgtgcg cgatggacga gtaggccagc
     2221 atgcgcttca cgttcgactg gacgagcgcc acgatgttgc cgtagaccat cgtgatgacc
     2281 gcgatgaccg cgagcaccgt ggtccatcgt tccgcgggga gcgccaggta cagcaccagg
     2341 atcagcgatc cgaaagccgc cgctttcgag gccgtcgcca tgaagcccgt cagcgtcgtc
     2401 ggcgctccct ggtacacgtc gggcgtccac atgtggaacg ggacggcgct gaccttgaag
     2461 aggaatccca ccatgagcag ggccgtgccc gcccagaaca gcgtcgaggt gccgttcagc
     2521 gccaggtatt cggcgatttc cggaaggtag aacgtgcccg tcgcgccgta gaggagggcg
     2581 atgccgtaca ggaagaaccc ggtggcgaac gcgccgagca ggaagtactt gagcgcgctt
     2641 tcgatggcgc cctcgtcgtc gcgcacgagc ccggtgatga tgtacaggca gacggacatg
     2701 gtctccagcc ccacgaagac cgtcaccagg ctgtttgccg cggccagcgt catcatcccg
     2761 agcgacgcga acaggatgat ggcgtacacc tcgccgtagt tgtgcttcat ccggcccagg
     2821 tacgacggcg agagcaggat cgtgagcccc gcgctgagca ggatgaccgt gttcagccag
     2881 gccgcgtacc ccccggtgcg gatcatgccg tagaaggccg tgcccgtcgg gtcgccgatc
     2941 ttctgcactt ccagaaccag gccggccacg acggccagga ggcccagcca cggaagcgag
     3001 gggtgatcgt tcctgaatgc gtcgacgacg acgaggacga gcccgacgag cgcgaccagg
     3061 acgagcgctt ccgcggcgaa caggtcaccg atcagggaat cgtatgctgt ggagagatcc
     3121 atggcgggat gtgcgttcag gcgaggcccc gggcctcaca cccaggaaat ggcttcgatc
     3181 cgccgggcca gctcggcatc ggcggcggtg atgcggtcgc ccgcggaatg cgtggtcagg
     3241 gcgacggtca cctggttgta cacgttcgtg agctcggggt gatgatccat gtcctcggcc
     3301 tcgaaggcga gtcggacgac gaagctcatc gcctcgcgga acgaaccgaa ggtgaacgtc
     3361 ttgatcaggc ggtcaccctc cgagccccag ccgggcaggc tggccagcgt tctgtcgagg
     3421 gcgtcaggtt ccaggggagt acggtcggtc atggggtcac ccgggtcaca ggacccatcc
     3481 gctgtagaag acgaggaaga tcacgaagta taccggcagg aggatcggaa tggagtagcg
     3541 gacgatgtac cccatgaaag agggcgtctc gaccccgttc gcctccgcga tggccttgac
     3601 catgaagttc ggggcattgc cgatgtacga catcgcgccg aagaagacgg cagccacgga
     3661 aatggcctgc aggaagaacc aggaatccgg gtgcgccatc tcgggcagcg cgaagtcccg
     3721 gacgg
//
LOCUS       DBNHQZ010000135        20854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2475918, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000135 DBNHQZ010000000
VERSION     DBNHQZ010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 20854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20854
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2475918"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="AC16YU_03420"
     CDS             <1..361
                     /locus_tag="AC16YU_03420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6064302.1"
                     /translation="HGSAESIPLDDACVDTVLVTYTLCTIPDAPAALREMARVLAPGG
                     RLLFCEHGMAPDRGVRRWQRLLGPAWRRLGGGCNLDRDIPSLIRDAGFTITGMHASYI
                     PGWRPASFNFRGAAVIP"
     gene            419..2137
                     /locus_tag="AC16YU_03425"
     CDS             419..2137
                     /locus_tag="AC16YU_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR6064303.1"
                     /translation="MSATARPVRHGPYPELSLPAILVGYLLGAVIAVSIGYAALILGF
                     SIEGSELAAILGFGILRGIMRRTSIVENNINQTIASAVNGASAGMMFSVPALFILGVT
                     EFSYPLMVLGCIAGAVLGIAFIIPLRKQMIDFERLTFPGGVAVATILKSPGAGMRKAA
                     LMLGGMAASALVYAVSLATGFENWNVGALVGMPEYMNGIWYVSLLTFGVAYIAGKGGF
                     YFIVGGYVCYWFLAPILAQTGALPIGGDGLPVTDPGALRGLLFRPVGIGMLIGGAVMG
                     IVLAFPLIVSAIRSMQESSRLQTALSRDEMPIRLLYGAVAGAAVVLFVIAVMSTSELG
                     WGRGLAMAVLGTLWIWMAGVILSECIGRTNWSPLSGMTLIAVTIFIFVASGIGDRGAI
                     IASVMVGAAACVAMAQATDLMLDLKTGYLVGATPRKQQIGQFLGSWIGPIIVMALIVL
                     LHEAEGLGSERLPAPQGTVLAGLIQGILGGDVPVEKYAAGAGLGALLSASGIGGLGIL
                     VGLGFYLPFNIVLTYSLGTLTRVVIDWRRGQRFSDDVGIPVAAGLIVGEALVGVAHAM
                     LKVFGG"
     gene            2142..2669
                     /locus_tag="AC16YU_03430"
     CDS             2142..2669
                     /locus_tag="AC16YU_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064304.1"
                     /translation="MKRTGLGLITLAFLGGAWLAVLDPVTLNWPLFLGIVVVGAAGAL
                     MARVAHRSHHGSEETVRAGTTQLRESLDAVVQAVESIRGDRLEVYAVSGAIDRRVLDH
                     LNRFVSVRDTMKVAYGLTGFAAVMGPYAGGERYLNRVWSASADGYVDEVEDYLGRALE
                     QFRLAREALRSLESA"
     gene            complement(2666..3655)
                     /locus_tag="AC16YU_03435"
     CDS             complement(2666..3655)
                     /locus_tag="AC16YU_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6064305.1"
                     /translation="MTGKLRWGLLSTARINGRLIEAVRASDRSEIAAVGSRTDEAAEA
                     FARERDIPRAYGSYEALVSDPDVDVVYLPLPNHRHAPWAVRAMRAGKHVLCEKPLALS
                     VVQVEIMARTAEREGRILAEAFMYRHHPRTVAARTLVRDGAIGAVRRVHGAFTFVFDR
                     DDNWRHDPEQGGGALWDVGCYPVSFARYVLEAEPVLVWAESTVTPSGIDTAMAGVLRF
                     PDDVLMTFDCGFEAAFRSEMTFVGTRGTIHVPLAFRPEGDAAILIERDGHVEERIVSG
                     YEPLFLGEVRDMEQAVLDGKRPAISLQDSRANVAALCALQSAAETGRPTAPEP"
     gene            complement(3652..4896)
                     /locus_tag="AC16YU_03440"
     CDS             complement(3652..4896)
                     /locus_tag="AC16YU_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6064306.1"
                     /translation="MATGRRLGVGFIGSGFIARFHIQSWQAVRDADVLGVWSPDPESA
                     ESAALLARSLRVGEARAFGSIAEMVRDPAIDCLWICGPNDRRIENMEEVVHALKHGGG
                     SLVGIACEKPLARNVGEAKRMVELIGEAGLLNAYLEDQLFSPGLVRGHAIAWNRGAAI
                     AGRPYLARAAEEHSGPHSPWFWQGAMQGGGVLNDMMCHSVEVARYLLTAPGAPRDSIR
                     PTKVTAQIASLKWSRPEYADDLLRRFGPEVDYRTRPSEDFARATIEYRDAEGRTLIAE
                     TTTSWSFVGAGLRLSSELLGPEYSMQLNSLDAGGRLFFSRRVTGKAGEDLVEKQNAEQ
                     GVMPFVGNEAAEYGYEDENRHFIRCFLDGRQPELGFDAGLEVVELLMTAYMSADEERT
                     IAWKPEGLERYIPPVAAGTWKP"
     gene            complement(5123..6391)
                     /locus_tag="AC16YU_03445"
     CDS             complement(5123..6391)
                     /locus_tag="AC16YU_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064307.1"
                     /translation="MRRIVLFLIVLAASAACAQAQNGARPSGLRLFLDCEGHGVCDQD
                     YIRTEINYVDHVRDRTQSDVHVLVTTEGTASGGRLFTLAFLGRGAYAAISDTLSYASL
                     GTDTWNELREGLTRMIAVGLVPYLARTALAGRLRLSVDPNGADRQTPVRADDPWNYWV
                     FGAGVSAEFEGEESQKDYRVRGNLSANRTTEDWKVRLSTRGDFRESRYSLEDTSIVSI
                     QRDGEAFGFVAASLGPHWSVGGSGFANTSTRRNIEYSVSGGPAVEYNIYPYSESTRRE
                     LRLQYRVDLEYVLYDEETIFDRYDETLLSHELEVRLDMNQPWGGTGASLEVTHYLTNF
                     DRSRTRYYSVEVDGGINVRIVRGLSVFFSGGVEFIHDQLYLSKEDVSDEDVLLGRVSL
                     PTSYEFRVEAGLNYSFGSIFNNVVNPRFGF"
     gene            6658..7854
                     /locus_tag="AC16YU_03450"
     CDS             6658..7854
                     /locus_tag="AC16YU_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064308.1"
                     /translation="MFTSFSDAVFDHLMTTVPAGAPVARQRLSQPDVPRPIGHFLDLS
                     LQRRVALQVTDLDRIAVGWVDHRTAEVAAGREAFVKVLVENAQFPVDEWPLALRQAVE
                     RVVSHLVRPVPTLVHFLFGEESGPVERDDLLRRSRYFVAHAYLGTTAEALLSRTGTGP
                     VNRRDLSEALERVERSVTSEWSTDDWFRALSPLLDLARLAELDGVPLAMLDAFFSAKD
                     APLLAARIHAYGKRKGRQALDGDEVRAALADATHAPETEPAGTPARAAAPAASGVPLW
                     QQFRPGSESPADQGAGAESGEPLWKRFREPAADGDRSASLHVLERDVLGAAGGKRDDF
                     VRDLFSGSEEEYGRILGRLRAATTWAAASRILGEDLFRKHKIDIYSPAAVEFTNAVEN
                     RYRRDS"
     gene            7912..9465
                     /locus_tag="AC16YU_03455"
     CDS             7912..9465
                     /locus_tag="AC16YU_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6064309.1"
                     /translation="MSESRTPLDELDALREEAGLGGGEDRIARQHAKGKLTARERIGL
                     LLDEGSFEETGTFVRHQSHDFGQERTRPLGDGVVTGYGTIKGRLVYVFSQDFTVFGGS
                     LGMAHAQKIVRLLRLAMENGAPVIGLNDSGGARIQEGVVSLGGYADIFLLNTLASGVV
                     PQISAVMGPCAGGAVYSPAITDFVFMVRDTSHMFVTGPNVVKTVTHEDVSFEDLGGAA
                     THATKSGVAHLACENDAECLARIRDLMDFLPQNCEEPPPDGPADDPAERKDAALDTVV
                     PDSPNRPYDMIDVIHRIVDHGRFFEIHAEYAANLIVGFAHLGGRAVGIVANNPGVLAG
                     VLDVNSSVKGARFVRFCDAFNIPLVVFEDVPGFMPGTDQEWQGIIRHGAKLLYAFSEA
                     TVPKITVITRKAYGGAYDVMNSKHIRADLNFAWPSAEIAVMGPKGAVEIIYRAQIAAS
                     DDPAAEEDRIIAEYREKFANPYVAAEHGYIDDVIRPSETRLRLIRGLDMLRNKVQSVP
                     RKKHGNIPL"
     gene            9608..11815
                     /locus_tag="AC16YU_03460"
     CDS             9608..11815
                     /locus_tag="AC16YU_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6064310.1"
                     /translation="MSRFAAAALLALPLLAGLVPTASGQPASRLRTLSPERLPVANSA
                     QALVGSTLVSADDLVESYRQARLDEFYELQADLDVAISVQDEDSAREVVAEVAKRMAP
                     LVRHPDMVGDGRFVDAYRSMIAGFEEEFGPVDPEFEVAFGDVFAVREELFSTLEGLAD
                     PLSPDMAPWHLPLQTVVPMTVNRAVTSSMEFLLERRPKIIATWLERAHTYFPMIEQIF
                     REEGIPEELKYLAMIESGLNPTARSRARAVGMWQFVAATGRAYGLKVDNWVDERRDPE
                     KATRAAARHLKDLYAIHGDWHVALAGYNCSPRCINRARSNARKAGSTNPGYWEMYRFL
                     PRETRGYVPNLIATAIMLSDPGAYGIRPDAPGFRYEYELVPIRGSLALSDVARMAGTD
                     VATIRQLNPSLLRNHLPPSRDPFPLRLPVGTSEAFLSAYAELPVEALKPPGEHVVKRG
                     NTLGAIASQYGVSVARLKADNNLRSDIIRVGQRLVVPVSSYQLPVDLDVSGTEAVVVR
                     YGELRNRRIVSTLEPPKRLPEASTATVAVAPSEDNAAAEPAPESRVPVVLASTAGEPS
                     DEEPAPEARTVVTYEVRRGDALSRIAGRYGVSVRDLQGWNDLRSTRIRAGQELTIYLD
                     GSAPQAESAAEPIIYRVRRGDTLGRIASNHGVPLSDLRSWNGIQGSVIRPGQRLRIYP
                     SGTGHEMRTHRVVRGDNLYELARRYGVTVDELRSWNELRSSRIYPGQSLRILR"
     gene            12013..12627
                     /locus_tag="AC16YU_03465"
     CDS             12013..12627
                     /locus_tag="AC16YU_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6064311.1"
                     /translation="MPDTARNPTSDEALIASYVERGDEGAMRTLVERHQQRIFGFLLG
                     MVRDREVANDLFQETFFRILSALRSERASYTDQGRFLGWAMRIARNAALDHLRSRGRW
                     LDVDQTGSEDGTSYWDRLTDDAPSAEQTMHREDQREWLRESIDRLPPDQREVVLLRHE
                     SELTFREIAELTGVSINTALGRMRYALINLRRMIPSEEIRTAES"
     gene            12645..13346
                     /locus_tag="AC16YU_03470"
     CDS             12645..13346
                     /locus_tag="AC16YU_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064312.1"
                     /translation="MDRKLQLIAHLYEEKVDGLEPLDELLRDPELRDEYRALRESRFA
                     LDHRSPVRPDARSIDAIMAAARTPSADRPPLRLVRIRRLMVPAMATAAVLLAAVLAVP
                     LMMTDSARGPLGVDEQAAAPQVAGEATGAPQAAVEEKAGVSPVENFLRSLPPAAPVPA
                     PAALASAEAVADADDDLTSWDSGRDVRRLSRRIATLRAAGVDEWDGEAVPLETLPDEA
                     GRNDLLPAGARRPGN"
     gene            13378..14265
                     /locus_tag="AC16YU_03475"
     CDS             13378..14265
                     /locus_tag="AC16YU_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064313.1"
                     /translation="MRAIPFKALRWGMLALVLGLVAAVPSAAQESHTVRIRDGAVFVD
                     GRRVPKDELPAALRASELQLHLEFTGPAHFHLDGVEYGIEDGRIVEVPPGEDRVVVFF
                     SGNQGELVEVPEPPGTALRLRLDGPPDGAIGTYLQVLDEQAAQFERIGEQIAAQRMDD
                     SAAMADRLREEAEQTAIMVRAFPRVEVQTYLQGIQNRDRSLYDQLMREQRLELASGDL
                     ARRIFATADPDSRASLTENLRRQLDEIFELKQENRRDEIAQLELQLQELREQLAAREA
                     NREAIIGRRLEALLANPHR"
     gene            14308..15099
                     /locus_tag="AC16YU_03480"
     CDS             14308..15099
                     /locus_tag="AC16YU_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064314.1"
                     /translation="MKRNRVMYTARGLAALAALWAGQLLLPVGALAQETGDETVREQR
                     VVVEVRDGKVYVNGKETAVGESGRIVLRKGDGDEVVVFTDEDGRAWVSAYGIGPRARA
                     DAERAREMARSMVRMHAPDFDAEDFVVEFEPPDLSELGNLELAIEGRLADVFEHQGNF
                     NGNVFRWRKQSQELRELEGEIRRKAREVRRADENDRAALEAELDALVDRAFDLKLEDE
                     RSGIADMERRLEELRDRMRDREANRREIVERRKKDLLGRRDNLDW"
     gene            15332..16540
                     /locus_tag="AC16YU_03485"
     CDS             15332..16540
                     /locus_tag="AC16YU_03485"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR6064315.1"
                     /translation="MAPMRVQVRIALFAAALLLVADARASTPTLERVSFTPSSSRPGT
                     VVRLHFSERIASFSLQQGEDQRSVRVTTVGADAPATLACDAVSRPFDACVIHHEAGGL
                     VIQLSLTGLLAAADVYRDARVRTDLLVYVETAGAPVVSAATSAAGSEAEAAAGEQAAR
                     LARSAAAQRIDTVVIDAGHGGKDPGTIGYRGIREKDIVLDIARRVGAYLEEEADVRVV
                     YTRSDDRFIELRERGEIALREGGKLFVSIHANSARNTRASGTETYFLGRQKSEAARAV
                     IERENSVIDLEDDRDHYRQFDAAQLVKVRMTESSNLMQSQELAQRIQFQYRERVGRQD
                     RKVKEGPFLVLWAAGMPAVLTEIGFISNPSEARFLASEQGKDYIASAIFRAVRDYKVA
                     FEAGLDVAAR"
     gene            16567..17139
                     /locus_tag="AC16YU_03490"
     CDS             16567..17139
                     /locus_tag="AC16YU_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064316.1"
                     /translation="MKPLPLRSALLVLVVGLTALAGCDSALTDPDVVILTHELTRSGD
                     GSAVRFSFDADDVNVGQSTVLRCGCTVDLDPWLASQGFSRDDLVAATVQSATLTKISP
                     PLERLDFLDSAVLRFEAAGLQAADVATRSVFPDAIDVALNPVAGRSVLAFASRDEFEP
                     VLVIEAADLQAGALYELEVEVTLRLELEGF"
     gene            complement(17151..17552)
                     /locus_tag="AC16YU_03495"
     CDS             complement(17151..17552)
                     /locus_tag="AC16YU_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6064317.1"
                     /translation="MRKKAVTVTRTRVKTPLAPAAIGPYSQAVLVGDMLYCSGQIAID
                     PKTGHLKASGIETETEQVLENLGAVLRAAGMDYRNVVRCTVFLADINDYAQVNEIYAR
                     YFSDDPPAREAVQVAGLPRGALVEMSCVAVR"
     gene            17649..18464
                     /locus_tag="AC16YU_03500"
     CDS             17649..18464
                     /locus_tag="AC16YU_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6064318.1"
                     /translation="MDLGLRDRVALVTGASRGLGLAAATELAREGCRVALCSRTRERI
                     DRAADSLAQGTGVPGDRVLPLTCDVTREEDVRRAVEATVERFGTLNVLVTNAGGPPSG
                     QVDDFDAEAWHAALELNLMSTINLCRHALPHLRRAAAEQDGLARIIMITSLAARQPVP
                     TLYLSNVARAGVQGFAKSLSEELGPAGITVNTILPGYTRTARLVELATSLEERTGRST
                     REIEAGWASGSALKRIGREDEFAAAVAFLAGSRASYITGVALPVDGGAIKSLF"
     gene            complement(18448..19167)
                     /locus_tag="AC16YU_03505"
     CDS             complement(18448..19167)
                     /locus_tag="AC16YU_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6064319.1"
                     /translation="MTYPLDYDPADIPSDSASLAHGRYLAESHGCTGCHAPDLGGAVA
                     IDARPFRVVASNLTSGSGGIGRTYTATDWLRSIRHGVRPNGDGLYMMPSSTYWYLSDE
                     EVGALVAYLKTLPAVDRELPPTEVKALGTVIAAIDRSIGPDLLAAHPERRRLPVPAER
                     TASAAMGAYRAWVLCSHCHGVDMHGGAHPDPAAPPVPDLVAVKGWTSDGFVRAMRTGE
                     TPGCHSRRRQGRTAGPLRTGS"
     gene            complement(19360..20166)
                     /locus_tag="AC16YU_03510"
     CDS             complement(19360..20166)
                     /locus_tag="AC16YU_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6064320.1"
                     /translation="MTTDAGAVRATLLGTGTSTGVPLVGCRCRVCTSPDPRDRRLRCS
                     CHLRAGGVSVLVDAGPDLRQQALRAGIRSVDAVFITHHHFDHVAGLDDLRPFLFANRR
                     AIPCFALPETARVLRRTHGYIFEDRSYPGVPDLVLHEVDGPFRIVSREDPSVGIDARA
                     VPAWHADLRVAALRIGRFGYATDVSRIPDDSREHLSDLDVLVLDALRREPHPAHFTID
                     EAVRVARSLRAAKTVLIHMTHTVLHAEEEVRLPAGVELGYDGMRLEAGLR"
     gene            complement(20156..20446)
                     /locus_tag="AC16YU_03515"
     CDS             complement(20156..20446)
                     /locus_tag="AC16YU_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064321.1"
                     /translation="MKTQQVVRELEDAARALGVSVRRERGSFRGGYCTRDDEELLMLN
                     KNHPPEVHLAILAEALRELPVDSVFIRPAARRALEDAWARRVDIEIGEQDDD"
     gene            complement(20497..>20854)
                     /locus_tag="AC16YU_03520"
     CDS             complement(20497..>20854)
                     /locus_tag="AC16YU_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064322.1"
                     /translation="QQTRGNVTLSLFEYQTLGAGSGIGARAERPRWIAEARVTPTVAF
                     ATRSFGGTVGFVWGIDAGAWLNMGPLVGPVGLAVGYGFSWKRWRVGGPEAVAVAGSGR
                     FNYTGSEHVLRLGLSW"
BASE COUNT         3191 a         7356 c         7103 g         3204 t
ORIGIN      
        1 ccatggcagc gcggagtcca tcccgctcga cgacgcctgc gtcgatacgg tgctcgtgac
       61 ctacacgctc tgcaccatcc ccgacgcccc ggccgcgctc cgggagatgg cccgcgtcct
      121 ggcgccgggc gggcggctcc tgttctgcga gcacggcatg gcgccggacc ggggcgtccg
      181 gcgatggcag cggctgctcg gacccgcgtg gcggcggctc ggtggagggt gcaacctgga
      241 ccgcgacatt ccctccctca tccgggacgc gggcttcacc atcaccggga tgcatgcgtc
      301 ctacattccc ggctggcgcc cggccagttt caacttccgg ggtgcggccg tcattccctg
      361 acgctacatt cgggcgcgtc ccgccgcctt tcgccgcgcc aaccgccctc cacagccgat
      421 gagcgccacc gcccgccccg tccgacacgg cccctacccc gagctctccc tgcctgccat
      481 cctggtcggc tacctgctcg gcgccgtgat cgccgtctcg atcggttacg cggccctcat
      541 cctcggcttc tcgatcgagg gatccgagct cgcggccatc ctcggcttcg gcatcctgcg
      601 gggcatcatg cgccgcacgt ccatcgtcga gaacaacatc aaccagacga tagcgtcggc
      661 cgtgaacggg gcgtcggccg gcatgatgtt ctcggtgccg gcgctcttca tcctcggggt
      721 gacggagttc agctatcccc tcatggtcct gggctgcatc gccggcgccg tcctcgggat
      781 cgcgttcatc atcccgctgc gcaagcagat gatcgacttc gagcggctga ccttcccggg
      841 cggcgtggcc gtggcgacga tcctcaagtc ccccggcgcc ggcatgcgga aggcggcgct
      901 gatgctcggc ggcatggcgg cgagcgccct cgtgtacgcg gtctccctcg cgacgggctt
      961 cgagaactgg aacgtcggcg ccctcgtggg catgcccgag tacatgaacg ggatctggta
     1021 cgtgtcgctc ctgaccttcg gcgtcgccta catcgccggc aagggcggct tctacttcat
     1081 cgtcggcgga tacgtctgct actggttcct ggcgccgatc ctcgcgcaga ccggcgccct
     1141 gcctatcggc ggggacggcc tgccggtcac cgatcccggg gcgcttcggg gcctgctctt
     1201 ccggcccgtc ggcatcggca tgctgatcgg cggcgccgtc atgggcatcg tgttggcgtt
     1261 tccgctcatc gtcagcgcga tccgatcgat gcaggaatcc tcccgcctgc agacggcgct
     1321 ctcccgggac gagatgccca tccggctgct gtacggcgcg gtggctgggg cggcggtcgt
     1381 gctcttcgtg atcgcggtga tgtcgacgtc cgagctcgga tggggccgcg gcctcgccat
     1441 ggcggtgctc ggaacgctct ggatctggat ggcgggcgtc atcctgtcgg aatgcatcgg
     1501 ccgcaccaac tggtcgccgc tctccgggat gaccctcatc gccgtcacca tcttcatctt
     1561 cgtcgcgagc ggcatcggcg atcgcggagc catcatcgca tcggtcatgg taggcgcggc
     1621 ggcgtgcgtg gccatggcgc aggccaccga cctcatgctg gacctgaaga cgggctatct
     1681 cgtcggcgcg acgccgcgca agcagcagat cgggcagttc ctgggcagct ggatcggacc
     1741 catcatcgtg atggccctca tcgtgctcct gcacgaggcg gagggcctgg gcagcgaacg
     1801 gctgcccgcg ccgcagggca cggtgctcgc cggcctgatc cagggcatcc tgggcggcga
     1861 cgtgcccgtc gagaaatatg ccgccggggc gggcctcggg gcgctgctct cggcgagcgg
     1921 catcgggggc ctcggcatcc tcgtcggcct cggcttctac ctgccgttca acatcgtgct
     1981 cacgtactcg ctgggcacgc tcacgcgggt cgtcatcgac tggcgccgcg gccagcgatt
     2041 cagcgacgac gtcggcattc ccgtggcggc cggcctcatc gtgggagaag ccctggtcgg
     2101 tgtcgcccac gccatgctca aggtgttcgg agggtaacgc catgaagcgg accggcctcg
     2161 gcctcatcac cctggcattt ctcggcggcg cctggctcgc ggtgctcgac cccgtaaccc
     2221 tgaactggcc cctgttcctg ggcatcgtcg tcgtcggggc ggccggcgcg ttgatggccc
     2281 gcgttgccca tcgcagtcat cacgggtccg aggagaccgt gcgggcgggc acgacccagc
     2341 tccgggagag cctcgacgcc gtcgtccagg cggtcgagtc catccggggt gaccgcctgg
     2401 aggtgtacgc cgtgtccggg gccatcgacc ggcgcgtgct ggaccatctg aaccggttcg
     2461 tctcggtgcg cgacaccatg aaggtggcct atggattgac cgggttcgcc gccgtcatgg
     2521 gcccgtacgc cgggggcgag cgctacctga accgcgtctg gtccgcgtcg gccgacgggt
     2581 acgtggacga ggtagaggac tacctcgggc gtgccctcga gcagttccgg ctggcgcgcg
     2641 aggcgctccg gtcgctggaa tcggcctagg gctcgggagc cgttggccgg cccgtttcgg
     2701 ccgccgactg cagggcgcac agtgccgcca cgttggcccg gctgtcctga agagaaatcg
     2761 ccgggcgctt tccgtccagc acggcctgct ccatgtcccg gacttcgccc aggaagaggg
     2821 gctcgtaccc ggacacgatc cgctcctcca cgtgtccgtc ccgctcgatc aggatggcgg
     2881 cgtctccttc cggccggaac gccagcggca cgtggatcgt gccccgggtg cccacgaacg
     2941 tcatctcgga acggaaggcg gcctcgaaac cgcagtcgaa cgtcatgagc acgtcgtcgg
     3001 ggaaccggag gaccccggcc atcgccgtgt cgatgccgga gggggtgacc gtgctctcgg
     3061 cccagaccag gacgggttcc gcctccagga cgtaccgcgc gaaggacacg ggatagcagc
     3121 ccacgtccca gagcgcgcct ccgccctgct ccggatcgtg ccgccagttg tcgtcccggt
     3181 cgaagacgaa ggtgaaggcc ccgtgcaccc ggcgaaccgc gccgatcgct ccgtcccgca
     3241 cgagggtgcg cgcagcgacg gttcttggat gatgccggta catgaaggct tcggcgagga
     3301 tgcggccttc ccgttcggcc gtccgggcca tgatctccac ctggaccacc gaaagcgcga
     3361 gcggcttctc gcacagcacg tgcttcccgg cgcgcatcgc gcgcaccgcc cacggggcat
     3421 gccggtggtt cgggagcggg aggtacacca cgtccacgtc gggatcggag acgagggcct
     3481 cgtacgatcc gtatgcgcgg gggatgtccc gttcccgcgc aaaggcttcg gccgcctcgt
     3541 ccgtccggct tccgaccgcc gcgatttcgc tccggtcgga ggcgcggacg gcctcgatga
     3601 gccggccgtt gatgcgcgcc gtggagagca ggccccagcg aagctttccg gtcatggctt
     3661 ccaggtgccc gccgcgaccg gcgggatgta gcgttcgagc ccttcaggct tccacgcgat
     3721 ggtgcgctcc tcgtcggcgc tcatgtacgc ggtcatgagc agctccacga cttccaggcc
     3781 ggcatcgaag cccagctcgg gctggcggcc gtccaggaag caccgtatga aatggcggtt
     3841 ctcgtcctcg tacccgtatt cggccgcctc gttgcctacg aacggcatga ccccctgctc
     3901 ggcgttctgc ttctcgacca ggtcctcgcc cgccttcccc gtcacgcggc gcgaaaagaa
     3961 caggcgtcca cccgcgtcga gcgaattgag ctgcatcgag tactcggggc cgagcagttc
     4021 cgagctcagg cgcaggcccg ctccgacgaa gctccaggag gtcgtggtct cggcgatcag
     4081 cgtccgtcct tcggcatccc ggtactcgat ggtcgcccgg gcgaagtcct ccgagggcct
     4141 cgtccggtag tccacttcgg gaccgaagcg gcgcaggagg tcgtccgcgt attccggccg
     4201 ggaccacttg aggctggcga tctgggccgt caccttcgtc ggccggatgg agtcgcgcgg
     4261 cgcgcccggc gcggtcagca ggtacctggc cacctcgacg ctgtggcaca tcatgtcatt
     4321 gaggacgccg ccgccctgca tcgcgccctg ccagaaccac ggcgaatggg gcccggaatg
     4381 ctcctcggcc gccctcgcca ggtacggccg gccggcaatg gccgccccgc ggttccaggc
     4441 gatcgcgtgc ccccggacga gcccgggcga gaacagctgg tcctccaggt aggcattcag
     4501 caggccagcc tccccgatga gctccaccat gcgcttcgcc tcacccacgt tgcgggcgag
     4561 cggcttctcg cacgcgatgc cgacgagcga tccgccgccg tgcttcagcg cgtgcaccac
     4621 ctcctccatg ttctcgatcc ggcggtcgtt cgggccgcag atccacaggc agtctatggc
     4681 cgggtcccgg accatctccg cgatggatcc gaaggcccgg gcctcgccga cgcgcaggct
     4741 ccgcgccaga agtgccgcgg actccgcgct ctccgggtcc ggggaccaga cgcccaggac
     4801 gtcggcgtcg cgcacggcct gccacgactg gatgtggaaa cgggcgatga agccgctccc
     4861 gatgaaccct accccgagtc gtcttcctgt cgccatggct ccgcgtgggt caacgttgcc
     4921 cggaagatag gcttccgaaa aacaggagcc cgtcgcttcg ggcggcgggc tcctcaccat
     4981 gaaggacact gcgagcggac gagtcctggg gtcgcccgcg cagcgcctcg ggtatcgtgc
     5041 ggatccatca tacgggatcg gccgatcccg ttccgccggg cggaccccgg aatccgcgta
     5101 ggacccccgc ccactcgggg gatcagaatc cgaaacgcgg attgaccacg ttgttgaaga
     5161 tggatccgaa gctgtagttc agccccgcct cgacccggaa ctcgtacgag gtcggaaggc
     5221 tcactctgcc cagcaggacg tcctcgtcgg agacgtcctc cttcgacagg tacagctggt
     5281 cgtggatgaa ttcgaccccc ccggagaaga acacgctcaa gccccgcacg atgcggacgt
     5341 tgatcccccc gtcgacctcg accgagtagt agcgggtgcg ggaccggtcg aagttcgtca
     5401 ggtagtgcgt cacctccagg ctcgctcccg tcccgcccca gggctggttc atgtccagcc
     5461 ggacctcgag ctcgtgcgag agcagcgtct cgtcgtaccg gtcgaagatg gtctcctcgt
     5521 cgtagagcac gtactccagg tccacgcggt actgcaggcg aagctcccgg cgcgtggact
     5581 cgctgtacgg gtagatgttg tactcgacgg ccggcccgcc cgacaccgag tactcgatgt
     5641 tccgccgggt cgaggtgttc gcgaagccgg atcctccgac cgaccagtgc gggccgaggc
     5701 tcgcggccac gaatccgaac gcctctccgt cacgctggat gctgacgatg gaggtgtcct
     5761 cgagcgagta acgggactcg cggaagtcac cccgggtgct gagccgcacc ttccagtcct
     5821 cggtggtccg gttcgccgac aggtttcccc ggacgcggta gtccttctgc gactcctcgc
     5881 cctcgaactc ggccgagact cccgctccga acacccagta gttccacggg tcgtccgcgc
     5941 ggaccggcgt ctggcggtcg gcgccgttcg gatccaccga gagccggagc cgcccggcga
     6001 gcgccgtgcg cgccaggtac gggacgagcc cgaccgcgat catgcgggtc agcccctccc
     6061 gcagctcgtt ccacgtgtcc gtgcccaggc tggcgtagga cagcgtgtcg gaaattgcgg
     6121 cgtaggcacc ccgtccgaga aaggccagcg tgaacagccg cccgccggac gccgtgccct
     6181 cggtcgtgac gagcacgtga acgtcggact gggtccggtc gcgcacgtgg tccacgtagt
     6241 tgatctccgt ccggatgtaa tcctggtcgc agaccccatg cccctcgcag tccaggaaga
     6301 gccgcagccc ggacggtctc gcaccgttct gggcctgcgc gcacgcggcc gatgcggcga
     6361 ggacgatcag gaagaggacg atgcgacgca cggagcggag ggctcggaag gtcatcacgg
     6421 gcgggcgaag ctaacgggcc ccgcgacgag gcgcccatcg agccattgaa gatgaggtgg
     6481 agaaggtgtg gcaatagcgt gtcgaaacca aacgcccggg cggtaccggg gcgttcccgg
     6541 aatcccggcg ccacggccga agtacatggt tccattcgcg ggcggcgacc gtggaacatc
     6601 cgcggcgcca tccgtttggc acctcggctc cctttcgccc ctcggcccat tcccggcatg
     6661 ttcacctcgt tcagcgacgc cgttttcgac catctgatga cgacggtccc ggcgggtgcg
     6721 cccgtcgctc gccagcgcct ctcccagccc gacgtacccc ggccgatcgg acatttcctg
     6781 gacctgtccc tccagcgccg cgtggcactc caggtgaccg acctcgaccg gatcgcagtg
     6841 gggtgggtgg atcaccgcac ggcggaggtc gcggccggcc gggaggcgtt cgtcaaggtc
     6901 ctcgtcgaga atgcccagtt tcccgtggac gaatggccgc tggccctccg gcaggccgtc
     6961 gagcgcgtgg tgtcgcacct ggtccggccc gtgcccacgc tggtccattt ccttttcggg
     7021 gaggagtcgg gaccggtgga gcgcgatgac ctgctgcggc gcagccggta cttcgtcgcc
     7081 cacgcgtacc tggggacgac cgcggaagcc ctgctctccc gcacgggtac gggaccggtc
     7141 aatcgccggg acctgtccga ggcgctggag cgggtcgagc ggtcggtcac gtccgaatgg
     7201 tccacggacg actggttccg ggcgctgagt ccccttctgg acctggctcg tctcgccgag
     7261 ctggacggcg ttccgctcgc gatgctcgac gccttttttt ccgcgaagga cgccccgctt
     7321 ctcgccgcgc gaatccacgc gtacggcaag cggaagggcc ggcaggccct ggacggcgac
     7381 gaggtccgcg ccgcgctggc cgacgccacg cacgcgcccg agaccgagcc ggccggaact
     7441 cccgcccggg ccgccgcacc cgcggcgagt ggggtgccgc tctggcagca gttcaggccc
     7501 ggcagcgaat ccccggccga tcagggcgcc ggcgccgagt cgggcgagcc gctgtggaag
     7561 cgtttcaggg agccggccgc cgacggcgac cgttcggcct ccctccacgt cctggagcgc
     7621 gatgtcctcg gggcggccgg agggaagcgc gatgacttcg tgcgggacct gttctccggc
     7681 tcggaggaag agtacggccg catcctgggc cgcctgcggg cggccacgac ctgggcggcg
     7741 gcctcccgca tcctgggcga ggacctgttc cgcaagcaca agatcgacat ctacagcccg
     7801 gcggcagtcg aattcaccaa cgccgtcgag aaccggtacc gccgcgattc ctgacggggt
     7861 gcgtgccgga tgccccgcgc cgcttccccg caatcccctg gagcacgaac gatgagcgaa
     7921 tcccggacgc ccctggacga gctggacgcc ctgcgcgagg aggccgggct gggcggaggc
     7981 gaggatcgca tcgcccgcca gcacgcgaag ggcaagctca cggcccgcga gcggatcggc
     8041 ctcctgctcg acgaggggtc attcgaggag acgggcacgt tcgtgcggca ccagtcacac
     8101 gatttcggac aggagcggac gcggcccctc ggcgacgggg tggtcaccgg gtacggcacg
     8161 atcaagggac gtctcgtcta cgtgttcagc caggacttca ccgtcttcgg cggctccctg
     8221 gggatggccc atgcgcagaa gatcgtccgc ctgcttcgcc ttgcgatgga aaacggagca
     8281 cccgtcatcg ggctgaacga ctcgggtggc gcccgaatcc aggaaggcgt ggtgtcgctc
     8341 ggcggctacg ccgacatttt cctgctgaac acgctggcct ctggcgtcgt gccccagatc
     8401 tcggccgtca tggggccctg cgccgggggt gcggtctaca gcccggccat caccgatttc
     8461 gtgttcatgg tccgggacac gagccacatg ttcgtcacgg gtccgaacgt ggtgaagacg
     8521 gtgacgcacg aggacgtgag cttcgaggac ctgggcgggg ccgccaccca cgccacgaag
     8581 agcggcgtcg cgcacctcgc gtgcgagaac gacgcggaat gcctggcccg gatccgggat
     8641 ctgatggatt tcctcccgca gaactgcgag gaaccgcccc cggacgggcc ggccgacgat
     8701 ccagccgagc ggaaggatgc cgccctcgac accgtggtgc cggacagccc gaaccggccg
     8761 tacgacatga tcgacgtgat ccaccgcatc gtggaccacg gccgcttttt cgagatccat
     8821 gccgagtatg ctgccaacct gatcgtgggc ttcgcccacc tcggggggcg tgccgtgggt
     8881 atcgtcgcga acaaccctgg ggtgctcgcc ggcgtcctgg acgtgaactc gtcggtgaag
     8941 ggtgcccggt tcgttcgctt ctgcgacgcg ttcaacatcc cgctcgtggt cttcgaggat
     9001 gtgcccggat tcatgccggg tactgaccag gagtggcagg gcatcatccg gcacggcgcg
     9061 aagctgctgt acgccttcag cgaggcgacc gtgcccaaga tcaccgtcat cacgcgcaag
     9121 gcctacggag gcgcctacga cgtgatgaac tccaagcaca tccgggccga ccttaacttc
     9181 gcctggccgt cggccgaaat agcggtcatg ggaccgaagg gcgcagtgga gatcatttac
     9241 cgggcacaga tcgcggcgtc cgacgatccc gccgctgaag aagatcggat catagccgaa
     9301 tacagggaga agttcgccaa cccatacgtg gccgcggaac atggatacat cgacgacgtc
     9361 atccggccgt ccgagacgcg cctccgactc atccgcggcc tggacatgct gcgcaacaag
     9421 gtccagtccg ttccgcggaa aaagcatggc aacatccctt tgtgaccccg cctacggggc
     9481 ggttcccggt gcccggttgg atggctccaa acccagccat tatattcgga taggccggat
     9541 cgccgagaat ctgcttgaag tgcggctggc cgtccgtagc ccttcggatt tcagcccgct
     9601 tcgagccttg agccgattcg cagccgccgc actcctcgcg ctcccgctcc tcgcgggact
     9661 cgtccccacc gcttccggcc agccggcctc gcgccttcgc acgctctcgc cggagcgtct
     9721 tcccgtcgcg aactccgccc aggccctcgt cggctccacc ctcgtgtcgg ccgacgacct
     9781 ggtggagagc tatcgccagg cgcgcctcga cgagttctac gagctgcagg ccgacctgga
     9841 cgtcgccatt tcggtccagg acgaggactc cgcccgcgag gtggtcgcgg aagtggcgaa
     9901 gcgcatggcg ccgctcgtgc gccatcccga catggtcggt gacggacgat tcgtggacgc
     9961 gtaccggtcc atgatcgccg ggttcgagga ggagttcggc ccggtggatc ccgagttcga
    10021 agtggcattc ggtgatgtgt tcgcggtccg cgaagagctg ttctcgacgc tcgaggggct
    10081 cgctgatccg ctcagtcccg acatggcgcc ctggcacctg ccgctgcaga cggtcgtgcc
    10141 gatgacggtg aaccgtgccg tgacgtcgtc catggagttc ctgctcgagc gccggcccaa
    10201 gatcatcgcg acgtggctcg agcgtgcgca cacctacttc ccgatgatcg agcagatctt
    10261 ccgggaagag gggatccccg aggaactcaa gtacctggcc atgatcgaga gcggcctgaa
    10321 cccgaccgcg cggagccggg cccgggcagt gggcatgtgg cagttcgtcg cggcgaccgg
    10381 cagggcatac ggcctcaagg tggacaactg ggtggacgag cgccgggacc cggaaaaggc
    10441 cacgcgggcc gccgcccggc acctgaagga cctctacgcg atccacggcg actggcacgt
    10501 ggcgctcgcg ggatacaact gcagcccccg gtgcatcaac cgggcccgtt cgaatgcgag
    10561 gaaggccggg tccacgaatc ccggctactg ggaaatgtac cgcttcctcc cccgcgagac
    10621 ccgtggctac gtgccgaacc tgatcgccac cgcgatcatg ctttcagatc cgggcgcgta
    10681 cggcatccgc cccgatgcgc ccggcttccg ctatgaatac gagctggtgc ccatccgggg
    10741 gagcctcgcc ctttcggacg tcgcccgcat ggccggcacc gacgtcgcca cgatccggca
    10801 gctcaacccg tcgctcctgc gaaaccatct cccgccgtcg cgcgacccgt tccctctccg
    10861 ccttccggtc ggcacctcag aggcgttcct ttcggcctac gcggagctgc ccgtcgaagc
    10921 gctgaagcct ccgggcgagc acgtcgtcaa gcggggcaac acgctcgggg caatcgccag
    10981 ccagtacggc gtctccgtcg cgcgcctcaa ggcggacaac aacctccggt ccgacatcat
    11041 ccgggtcggc cagcggctcg tggtccccgt gtcgagctac cagcttccgg tcgacctgga
    11101 cgtcagcggc accgaggccg tggtcgtccg gtacggcgaa ttgcggaacc ggcgcatcgt
    11161 ttcgaccctc gagccgccga agcgccttcc cgaggcttcg acggcgaccg ttgccgtggc
    11221 tccctcggag gataacgctg ccgcggagcc cgctccggag tcgcgcgtcc ctgtcgtgct
    11281 cgcgagcacg gccggtgagc cgtccgacga ggagcccgcc ccagaggccc gcaccgtcgt
    11341 gacgtacgag gtccggcgcg gcgatgccct gtcgaggatc gcgggccggt acggcgtgag
    11401 cgtccgcgac ctgcagggat ggaacgatct ccggtccacc cgcatccggg ccggccagga
    11461 gctgaccata tacctggacg gcagcgcccc gcaggcggag tcggccgcgg agccgatcat
    11521 ctaccgcgtc cggcggggcg acaccctggg gcggatcgcc tcgaatcacg gcgtgccgct
    11581 ttcggacctc cgttcgtgga acggcatcca gggatccgtc atccgtcccg gccagcggct
    11641 gcgcatctac ccgtcaggga ccggccacga gatgcgtacc caccgcgtcg tccgggggga
    11701 caacctgtac gagctggccc gtcgctacgg cgtaacggtg gatgaactcc ggtcctggaa
    11761 cgaactccgc tccagccgca tctatccggg ccagtccctg cggatcctgc gctgacggtt
    11821 tttccggccc ctttccgggg cagacctgcg gccgagtgtt ccgcgcattt ccggcgcccg
    11881 gcaggccggt gctccgtgca tctctggagc ccggcaggcg taccctccgc gtgaccacac
    11941 aataacgcgg gcagggcatt cgttcttggt gggaggtcac gaaacctcca cgaaccccga
    12001 ccgccgcgct ccttgccaga cacggccaga aacccgacga gcgacgaggc gctcatcgca
    12061 tcctacgtgg agcgcggtga cgagggcgcc atgcgtacgc tggtggaacg ccaccagcag
    12121 cgcatcttcg gcttccttct cgggatggtc agggatcggg aggtggccaa cgacctcttc
    12181 caggagacgt tctttcggat tctgtcggct ctccgcagcg agcgcgcatc gtacaccgac
    12241 caggggagat tcctgggctg ggccatgcgc atcgcccgga atgccgcact ggaccacctg
    12301 cgcagccggg gacgctggct ggacgtggac cagaccggat ccgaggacgg aacctcgtac
    12361 tgggacagat tgacggacga cgcaccctcc gccgaacaga ccatgcaccg cgaggatcag
    12421 cgagaatggc tccgcgaaag catagaccgg cttcccccgg accagcgcga ggtcgtgctg
    12481 ctccggcacg agtcggagct gacgttccgc gagatcgccg agttgacggg cgtgtccatc
    12541 aacacggcgc tcggccggat gcgctacgcg ctgatcaatc tccggcggat gatcccgtcg
    12601 gaggaaatca ggaccgcaga gtcttgacgg ctccccccga ccccatggac agaaagctac
    12661 agctgatcgc gcacctctac gaggagaagg tcgacggact cgagccgctc gacgagctgc
    12721 tccgggatcc ggagctccgc gacgaatacc gggcgcttcg cgagtcccgg ttcgcccttg
    12781 atcaccgctc cccggtccga ccggacgcca ggtcgatcga cgccatcatg gcggcggccc
    12841 gaaccccgtc ggcggaccgc ccaccgctcc gtctcgtgcg gatccggcgc ctgatggtcc
    12901 cggccatggc caccgccgcc gttctcctgg cggccgtgct cgcggtgccc ctgatgatga
    12961 ccgattcggc ccgcggaccg cttggagtcg acgagcaggc cgcagcgccg caggtggccg
    13021 gcgaagcgac cggcgctccg caggccgccg tcgaggagaa ggccggcgtc tccccggtcg
    13081 agaacttcct ccggtccctg ccaccggctg cgcccgtacc ggcgcctgcg gcgctggcct
    13141 cggccgaggc cgtcgcggat gcggacgacg acctgacctc gtgggacagc ggacgcgacg
    13201 tgaggcggct ttcccggcgc atcgcgaccc ttcgcgccgc cggcgtggac gagtgggacg
    13261 gggaggccgt cccgctggag acacttcccg acgaggccgg gcggaacgac ctcctaccgg
    13321 cgggagcccg ccggcccggc aactgaacac tcccttcccc aacattccct ggcaccgatg
    13381 agagccattc cgttcaaggc attgcgatgg ggcatgctgg cgctcgtgct cggactcgtc
    13441 gcggccgtgc cctcggccgc gcaggagagc cacaccgtcc gcattcggga cggggcggtc
    13501 tttgtggacg gccgccgcgt gccgaaggac gagcttcccg ccgcgctccg cgcttccgaa
    13561 ctgcagctcc acctggagtt caccggaccc gcccatttcc acctcgacgg cgtcgagtac
    13621 ggcatcgagg atgggcggat cgtggaggtt ccgccgggcg aagaccgggt ggtcgtcttc
    13681 ttcagcggga accagggcga gctggtcgag gtgcccgaac ccccgggcac ggcgctgcgc
    13741 ctcaggctgg acggtccgcc ggacggggcg atcggtacct atctccaggt gctcgacgag
    13801 caggccgccc agttcgagcg gatcggcgag cagatcgcgg cgcagcgcat ggacgattcc
    13861 gccgccatgg ccgaccggct gcgcgaggag gccgagcaga ccgcgatcat ggtgcgggcc
    13921 ttcccgcgcg tcgaggtgca gacgtacctc cagggcatcc agaaccgcga ccggtcgctc
    13981 tacgatcagc tcatgcgcga gcagcgcctg gagctcgcct cgggcgacct ggcgcgccgc
    14041 atcttcgcga ccgcggaccc cgattcacgg gcctcgctca cggagaatct tcgcaggcag
    14101 ctcgacgaga tcttcgagct caagcaggag aaccggcgcg acgagatcgc ccagctggag
    14161 cttcagctgc aggagcttcg cgagcagctg gcagcgcgcg aggccaaccg tgaggccatc
    14221 atcggtcgac gcctggaggc cctgctcgcg aatccccatc ggtagacggc gcaatccggc
    14281 gcacccaacc atccaatcga acgaaccatg aaacgaaacc gagtgatgta cacggcgcgc
    14341 gggctggccg cgctggctgc cctctgggcg ggccagctgc tccttccggt cggcgcgctc
    14401 gcccaggaga ccggggacga gaccgtccgc gaacagcgtg tcgtcgtcga ggtaagggac
    14461 ggcaaggtct acgtgaacgg caaggaaacg gccgtcggag aatccggccg gatcgtgctc
    14521 cgcaaggggg atggcgatga agtggtcgtc ttcacggacg aggacggcag ggcctgggtt
    14581 tcggcctacg gaatcgggcc gcgggcccgt gccgacgccg agcgtgcccg cgagatggcc
    14641 cgttcaatgg tccgcatgca cgccccggac ttcgacgcgg aggacttcgt cgtggagttc
    14701 gagccgcccg acctgtccga gctcggaaac cttgaattgg cgatcgaggg ccggctggcc
    14761 gacgtcttcg aacaccaggg gaacttcaac ggaaacgtat tccggtggcg gaaacagtcc
    14821 caggagctcc gtgagctcga gggcgagatc cggcgcaagg cccgcgaggt ccgccgggcg
    14881 gacgaaaacg accgggccgc tctcgaggcc gaactcgacg cgctcgtgga ccgcgcgttc
    14941 gacctgaagc tggaggacga acggtccggg atcgcggaca tggagcgtcg gctcgaggaa
    15001 ctgcgagacc gcatgaggga ccgcgaggcc aaccggcgcg agatcgtgga acgtcgcaag
    15061 aaggacctgc tcggccggcg cgacaacctg gactggtgac ccggaaaggc tttcccttcg
    15121 gaggaagaaa ccccgtgccg gccgggatcc gtatcccggc cggccggttc gttccggtgt
    15181 ccgccggttc gctttcgacg tccgatggcc gatattgcgt caacggacgg gcgaagtcga
    15241 gccgcccggc accgagtttg ttcgtgagga gaccgggcgc gagctccgga gggtccggaa
    15301 ccgcgcgtgc cggctcgaac ccttcgtccc catggccccg atgcgtgtcc aggtccgcat
    15361 agccctcttc gcggcagccc tgctgctcgt cgcggatgcg cgggcctcga ccccaaccct
    15421 ggagcgcgtc tcgttcaccc cgtcctcgtc gaggccgggc acggtcgtcc ggctccattt
    15481 ctccgagcgg atcgcgagct tcagcctgca gcagggcgag gaccagcggt cggtgcgcgt
    15541 taccaccgtg ggggccgacg cgcccgctac cctcgcctgt gatgcggtct ccaggccgtt
    15601 cgacgcgtgc gtcatccacc acgaggcggg cgggctcgtg atccagctct ccctcaccgg
    15661 ccttctggcc gccgccgacg tctaccggga cgcccgcgtc cggaccgacc tgctcgtgta
    15721 cgtggaaacg gccggtgcgc ccgtcgtgtc ggcggccacc tcggcggccg gatcggaggc
    15781 ggaggcggca gcgggagagc aggccgcgcg cctcgctcgc tcggccgccg cgcagcgcat
    15841 cgacacggtc gtgatagacg ccgggcacgg cggcaaggac ccgggaacca tcggctaccg
    15901 ggggatccgg gagaaggaca tcgtgctgga catcgcccgc cgggtgggcg cctacctgga
    15961 agaggaagcg gacgtgcggg tggtgtacac gcgctccgac gaccgcttca ttgagctccg
    16021 cgaacggggc gagatcgccc tccgcgaggg gggcaagctc ttcgtatcca tccacgccaa
    16081 ttcggccagg aacaccaggg cgtccggaac cgagacgtat ttcctgggca ggcagaaatc
    16141 cgaggcggca cgcgcggtca tcgagcggga gaacagcgtg atcgacctcg aggacgaccg
    16201 ggatcactac cgccagttcg acgccgccca gctcgtcaag gtgcgcatga ccgagagctc
    16261 caacctgatg cagagccagg agctggccca gcgcatccag ttccagtacc gggagagggt
    16321 cggacgccag gaccggaagg tgaaggaagg gcccttcctc gtgctctggg ctgccgggat
    16381 gcccgccgtt ctgaccgaga tcgggttcat ctcgaatccg tccgaagccc ggttcctggc
    16441 cagcgagcag ggcaaggatt acatcgcttc ggcgatcttt cgcgcggtac gtgactacaa
    16501 ggtggcattc gaggccggcc tggacgtggc cgcccggtga accgatagac agccttccat
    16561 ccgcccatga agccgctccc gctgcgctcc gctctcctgg ttctcgtcgt cggcctgacc
    16621 gccctggccg ggtgcgactc ggccctcacc gacccggacg tggtcattct gacgcatgag
    16681 ctcacgcggt ccggtgacgg ctccgccgtc cgcttctcat tcgacgccga cgacgtgaac
    16741 gtcggccagt ccacggtgct gcgatgcggc tgcacggtgg acctggatcc gtggctcgcc
    16801 tcgcagggct tcagccgcga cgacctggtg gccgcgaccg tccagtcggc tacgctgacg
    16861 aagatttcgc cgcccctcga gcgcctggac ttcctcgaca gcgccgtgct ccggttcgag
    16921 gccgccggcc tgcaggcggc cgatgtggcc acgcggtcgg tcttccccga cgcaatcgac
    16981 gtcgccctga acccggtcgc cgggcgctcg gtcctcgcct tcgcctcgcg tgacgagttc
    17041 gagcccgtgc tcgtaatcga ggccgcggac ctccaggccg gcgccctgta cgaactggag
    17101 gtcgaggtca cgctccgcct ggagctcgag ggcttctgag aaccggggag ctaccggacc
    17161 gccacgcagg acatctcgac cagcgccccg cgaggcagcc cggccacctg gaccgcctcg
    17221 cgcgccgggg ggtcatcgct gaagtaccgg gcgtagatct cgttgacctg ggcgtagtcg
    17281 ttgatgtccg cgaggaagac cgtgcaccgg accacgttgc ggtagtccat tcccgccgca
    17341 cgaagcaccg cgccaaggtt ttccaggacc tgctccgtct ccgtctcgat gccgctcgcc
    17401 ttcagatgcc cggtcttcgg gtcgatggcg atctgtcccg agcagtacag catgtcgccg
    17461 acaaggaccg cctggctgta cgggccgatg gcggccggcg cgagcggagt cttgactcgg
    17521 gtccgggtga cggtcacggc cttctttcgc atcgacacgg tgggtgcggg gagacgaacc
    17581 gcaaagtacc gtaacttgtg ccccgtcccg gtgtcaaacg gacgaattct actcaaagaa
    17641 cacctgccat ggacctcgga ctcagggacc gcgtcgcact cgtcaccgga gcctcccgcg
    17701 gtctcgggct ggctgcggcg acggagctgg cccgcgaagg atgccgggtc gcgctctgtt
    17761 ccaggacccg ggagcggatc gatcgggccg cggactccct ggcgcagggc accggcgtac
    17821 ccggcgaccg cgtgctcccc ctgacgtgcg acgtgacgcg cgaggaggac gtccggcgcg
    17881 ccgtcgaggc cacggtcgag cgcttcggca cgctgaacgt cctcgtgacc aacgcgggcg
    17941 gccctcccag cggccaggtg gacgatttcg acgccgaggc gtggcacgct gcgctcgagc
    18001 tgaacctgat gagcacgatc aatctctgcc ggcatgcgct cccccacctg cggcgggccg
    18061 cggcggagca ggacggcctg gcgcgcatca tcatgatcac gtcgctggcc gcgaggcagc
    18121 ccgtgcccac cctctacctg tccaacgtcg cgcgcgcagg cgtccaggga ttcgccaaga
    18181 gcctgtccga ggagctcggc ccggcgggca tcacggtcaa cacgatcctg cccggttaca
    18241 cgcgcaccgc ccggctcgtc gaactcgcca cgtcgctcga ggagcgcacc ggccgatcga
    18301 cccgggaaat cgaggccggg tgggcctccg gaagcgcgct caagcgcatc gggcgcgagg
    18361 acgagttcgc ggcggccgtg gcctttctcg ccgggagccg cgcctcgtac atcaccggcg
    18421 tggcgctccc ggtggacggt ggcgccatca agagcctgtt ctgagcggcc cggcggtccg
    18481 cccttgcctg cggcgcgaat ggcatccggg cgtctccccc gtccgcatgg cccggacgaa
    18541 cccgtccgac gtccatccct tgaccgcaac gaggtccgga accggcgggg ctgccggatc
    18601 ggggtgcgcg ccaccgtgca tgtccacgcc gtgacagtga gagcacagca cccaagcccg
    18661 gtaggctccc atggcggcag acgcggtccg ctccgcgggc acgggcaatc tccggcgttc
    18721 cgggtgggcg gccagaaggt cgggaccgat cgaacggtcg atcgccgcga tcaccgtgcc
    18781 cagggccttg acctccgtcg gcgggagctc ccggtcgacc gccggcaggg tcttcagata
    18841 ggcgacgagc gctccgacct cctcgtcgct caggtaccag tacgtggagg agggcatcat
    18901 gtagagacca tccccgttcg gacggacgcc gtgacggatt gagcgcagcc agtccgtggc
    18961 ggtgtacgtg cggccgattc cgcccgagcc cgacgtcagg ttggaggcga cgacgcggaa
    19021 cgggcgcgcg tcgatcgcga cggcgccgcc gaggtccggc gcgtggcagc ccgtgcagcc
    19081 gtgcgactcg gccaggtacc ggccgtgggc cagcgaggcg gagtcggacg ggatgtccgc
    19141 cgggtcgtag tcgagcggat aggtcaggcc cgggagcttc ctgcggcgaa cagcgcagcg
    19201 gaggcgagga ccaggatggc gacgaggccg acgacgacac gtgcggccac acgggcaacg
    19261 gctgccatga cgggttcggg ttgggggtag tcgggcagcg gggggtaacc ggtcgcatcc
    19321 tgcggcgcgc ggccagaccg ggttgcagcc ccgactgacc tagcggagcc ccgcctccag
    19381 ccgcatgccg tcgtatccga gctccacccc ggcgggaagc cggacctcct cctccgcgtg
    19441 cagcacggtg tgcgtcatgt ggatgaggac ggtcttcgca gcccggagcg accgggcgac
    19501 gcgcacggcc tcgtcgatcg tgaagtgggc ggggtggggc tcccgccgga gcgcatccag
    19561 caccaggacg tccaggtccg acaggtgctc ccgggaatcg tccggtatgc ggctcacgtc
    19621 cgtcgcgtag ccgaatcgtc cgatccggag cgccgccacc ctgaggtccg catgccaggc
    19681 cggcaccgcc cgggcgtcga tccccacgga tggatcctcc ctcgacacga tccggaacgg
    19741 cccgtcgacc tcgtgcagca ccaggtccgg aacgccgggg tacgaccggt cctcgaagat
    19801 gtagccgtgc gtccggcgaa gcacgcgggc ggtctcgggc agcgcgaagc aggggatggc
    19861 gcgccggttc gcgaacagga agggacgcag gtcgtccagc cccgccacgt ggtcgaagtg
    19921 atggtgcgtg atgaacacgg cgtcgaccga ccggatgccc gcgcggaggg cctgctggcg
    19981 caggtcgggg cccgcgtcga ccaggacgga cacgcccccg gcgcgcaggt ggcaggagca
    20041 gcggagccgg cggtcacgcg gatcgggcga cgtgcacacg cggcatcgac agcccacgag
    20101 gggaacgccg gtcgacgtac ccgtgcccag cagggtcgcc cgaaccgccc ccgcgtcagt
    20161 cgtcatcctg ctcgccaatc tcgatgtcca cccgccgggc ccacgcatcc tccagcgcgc
    20221 gccgggcggc cggccggatg aacacgctgt cgaccggcag ctcgcgcagg gcctcggcca
    20281 ggatcgccag gtgcacctcg ggagggtggt tcttgttgag catgaggagc tcctcgtcgt
    20341 cgcgcgtgca gtaccccccg cggaagctgc cccgctcccg gcggacgctg acgcccagcg
    20401 cccgcgccgc atcctccagt tcgcgcacca cctgctgcgt cttcatgccg gattacggtc
    20461 gtttctcggg gagccaatgt acggagcggc ggacgcttac cagctgagac ccagccgaag
    20521 tacgtgctcg cttcccgtgt agttgaaccg tccgcttccg gcgacggcga ccgcctcggg
    20581 tcccccgacg cgccaccgct tccaggagaa accgtacccc acggcgagac ccacggggcc
    20641 gacgagcgga cccatgttca gccaggcccc cgcgtcgatg ccccagacga agcccaccgt
    20701 gccgccgaac gaccgggtgg cgaacgccac cgtcggcgtg accctcgcct cggcgatcca
    20761 gcggggccgc tcggcgcgcg ccccgatgcc cgacccggcc ccgagcgtct ggtattcgaa
    20821 gagggaaagc gtcacgttgc cgcgcgtctg ctgg
//
LOCUS       DBNHQZ010000136        10614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2881765, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000136 DBNHQZ010000000
VERSION     DBNHQZ010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2881765"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /gene="pnp"
                     /locus_tag="AC16YU_03525"
     CDS             <1..2035
                     /gene="pnp"
                     /locus_tag="AC16YU_03525"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6064323.1"
                     /translation="TSRLVDRAIRPLFTDGFFNEVQVIINVLSAEPEVDADVLAGVGS
                     SAALLLAGAPFDGPIAEVRIGRVDGAFVVNPQISELEESDFDLVVAGKSDAIVMVEGE
                     MREVSEEDMLEALDIAHEAIRKLCAAQVQFVQEAGGPKPFSYVPRVLPEGLLDKVRGI
                     VGDRLAKHVRAPYEKSTFYGGISEIKEDVLTALLGADDPASGAVRAAATGEGWTAEQI
                     KEAVGTIERDVMRHMILDEGKRIDGRGFADIRDIWAEVGYLPRVHGSAIFTRGETQVL
                     ASVTLGTSKDVQPVDQIFDTTDKRFYLHYSFPPFSTGEAKFLRGPGRREIGHGYLAER
                     ALSGMLPDSEDFPYTIRLNADVLESNGSSSMASVCSGALAMMDAGVPLRKPVAGVAMG
                     LISEGGKTAVLSDILGTEDHLGDMDFKVTGTRDGVTACQMDIKISGLSRDLLKTALMQ
                     ANAGRMHILDVMDQTLAEPRPDLSPYAPRLTQITIDAGFIGAVIGPGGKIIQGIQRET
                     NTSIDITEEEGVGIVTIAATNEDDAMRALEIIRGIVTVPEEGEEYEGTVRKVEAFGAI
                     IEIMPGKEGLLHVSELDYGFVDRVEDYLQVGDKVKVKLIEVRDDGKLRFSRKPFLEKP
                     EGWEERPRERRDGQRSGGGGGRGGDRRRGGGDRRGGGDRRSGGGGGRGGDRRG"
     gene            2153..3406
                     /locus_tag="AC16YU_03530"
     CDS             2153..3406
                     /locus_tag="AC16YU_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6064324.1"
                     /translation="MSPRSFPEFERTTLENGVRVVTETIPSVRSISVGAWVHTGSRDE
                     AGGETGISHFIEHMVFKGTRRRRMHQIAQRMESVGGYLNAFTAKEYTCYYARGLDEHL
                     ARAVDTVCDLVMSPSFPEREVAKEKDVVVEEMRMYEDAPEELIFDRFDAMLYPDHPMG
                     RPVIGYEDTVRSFSREQLLAYMGDHYTLDRMVVAVAGNVSHRRVVTLVRQALQGTNRA
                     VSHTVRAPVAGYRTDRLVEQRNIQQAHLVLGTRGYDVFDDRRIALSVLNTIVGGGMSS
                     RLNQNIRERYGYCYNIYSFTNQYSDTGDFGVYMGTDAGKVDRAEKLIFRELDRLANTP
                     VSPRMLSQAISQVKGSIMLGLESMGSRMMRLGKQELYYRRYFTLDEVLEQVSAVSRED
                     VLRTAQELFRPDQFSRVILMPGNAA"
     gene            3403..4356
                     /locus_tag="AC16YU_03535"
     CDS             3403..4356
                     /locus_tag="AC16YU_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6064325.1"
                     /translation="MTHKPTRKVLVTGGAGFIGSHVADALVERGFKVHVLDDLSSGLR
                     SNVPEHAVLHELDVRDPAVADLFRSERFEILVHHAAQMDVRRSVADPVFDADVNLRGL
                     LNLMEAGRTSGLKKVIFASTGGAIYGEPAYVPQDEEHPLRPLSPYGVTKLASEKYLYY
                     YKHVHDIPFVALRYGNVYGPRQNPHGEAGVVAIFCNLILDGRQPVINGDGEQTRDYVF
                     VSDVVKANLAALDSKHTGVFNVGTGLETSVNRLFSRLRDELEPAVREEHGAAKAGEQR
                     RSVLSFERARNLLGWQPVVNVKDGLRMTAAWFRDHHGADGS"
     gene            4362..5093
                     /locus_tag="AC16YU_03540"
     CDS             4362..5093
                     /locus_tag="AC16YU_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6064326.1"
                     /translation="MYRIRLQQFEGPLDLLLFFIRRDELDVYDIPIARIADEFLEYVR
                     VLEEVDLDGVGDFLYMAALLISIKARLLLPHQETDEEGEPIDPRQELVERLLEYIRFK
                     EAAGHLERRFENRHEQYTRGFAPEDLVQTGEGSEQLVDATVFDLIGALRRVLTQAPEE
                     PEHTVVAESFSVEEQQQFLRESLGTERRSFVELIGGRTKAFVISTFLAVLELARMAEL
                     QIFVESGADDFYLVLIPHIESPTDP"
     gene            5090..5797
                     /gene="scpB"
                     /locus_tag="AC16YU_03545"
     CDS             5090..5797
                     /gene="scpB"
                     /locus_tag="AC16YU_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR6064327.1"
                     /translation="MTDRPEPDQDREAWSGVGSTRIERVVEALIFAADEALPARRIAG
                     IFAEVTGAERPGDADVEHAVDTLNTRYEAQDRPLRIERWSGGFRMATVPDVAPFVKAL
                     FHSGQVHRLSRSLMETLAILAYRQPVTKPEVDFVRGVDSDYAVRRLLELGLVDVRGRA
                     DSVGRPLLYGTTDRFLELFGLNALGDLPNLREIESILDDPSFHRERARLLMKAGGLTG
                     LLPDDAAPDASEELSDG"
     gene            5994..6713
                     /locus_tag="AC16YU_03550"
     CDS             5994..6713
                     /locus_tag="AC16YU_03550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6064328.1"
                     /translation="MRLNRFIARSGVCSRREADEWIVAGRVQVNGKVVTELGTRVAEG
                     DRVVVNGRDVSPRRAEYFLLNKPHDTITTTEDERGRRTVLDLLEVPAERKGALFPVGR
                     LDRGTTGVLLITSDGELAHRLMHPSYEIDKLYRVLTAEDVTPEQLEALVTGVPLDDGP
                     ARADRAEYVALPDRRQIGLSLHEGRNRQVRRMFEALGHRVVDLERVQYAGLTTRGVRS
                     GHWRRLTDREVTRLYKLVKLK"
     gene            6722..8224
                     /gene="guaB"
                     /locus_tag="AC16YU_03555"
     CDS             6722..8224
                     /gene="guaB"
                     /locus_tag="AC16YU_03555"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6064329.1"
                     /translation="MEHKVLSEVDRTLAAKIRGEGLTYDDVLLLPARSTVMPREATLA
                     TRLTRRIALNIPLVSAAMDTVTESDMAIALAREGGVGVLHKNMSIDRQAAEVRRVKRS
                     ESGMILDPITLKPGDTVADARAQMARYSIGGIPIVDDDNRLVGIATNRDLRFQSDGTV
                     RLDVVMTRAPLVTAPMGTTLEAAEALLQRHKIEKLPVVDEQDRLVGLITFKDIQKKRQ
                     FPLACKDEHGRLRVGAAVGVTADVLERVSALVAAGVDFVTVDTAHGHSMGVMRTIERV
                     KSSFADLDVIAGNVATSEATRELIEVGADAVKVGIGPGSICTTRIVAGVGVPQLTAVM
                     DCAAVGRELDIPIVADGGIKHTGDIAKALAAGAESVMIGGLFARVEESPGETILYEGR
                     KFKSYRGMGSIGAMEAGSRDRYFQDVEDDIRKLVPEGIEGRVPYSGTLHEVVYQMVGG
                     LRAAMGYCGCGDLEEFFERSRFVRITDAGVNESHPHNVHITKEAPNYTSR"
     gene            8228..9307
                     /locus_tag="AC16YU_03560"
     CDS             8228..9307
                     /locus_tag="AC16YU_03560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6064330.1"
                     /translation="MASERDSPEGRIEARRRELGVDALLIHDPADLRWASGFTGSNGL
                     LLWGSGARHFVTDGRYAVQAAAEVPGAQVHVPGYDLFGFLSEGGLADGLRRVAFASDL
                     LPVDTWKRLRSLLPDIDWVGVTALLSEARAHKTDAEVETIRRAQRLAERVFSEVLGLM
                     RPGMTEREVAAELTGRSLRGGADCMAFDPIVAVAARSALPHARPTDRAIAPGDPVLVD
                     FGCTVQGYASDMTRMVSFGPPSDALVRVYGIVSAALGAAEASIVPGVSCRDVDLAARR
                     VIEEAGHGDRFPHSLGHGVGLSVHEYPAVSWRNEDPLPEGSVVTLEPGIYLPGAFGVR
                     IEDMVRVLPTGPERLTAVDRDLVIL"
     gene            9311..9970
                     /locus_tag="AC16YU_03565"
     CDS             9311..9970
                     /locus_tag="AC16YU_03565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6064331.1"
                     /translation="MADFIPYRHPLACSDEEARDRAAAFRSLMQERRTVRDFSDRPVP
                     REVVVDCLATAGSAPSGANRQPWHFVVIEDADLRRRVREAAEAEERAFYEGRAPSDWL
                     EALEPFGTDARKPFLEIAPVLIAVFAERWGEEGGSRVKNYYVQESVGIACGMLIAALH
                     QAGLCTLTHTPSPMGFLSDVLGRPDREKPYLLVVAGHGTEGVAVPDIARKPLEQTVSF
                     R"
     gene            9979..>10614
                     /locus_tag="AC16YU_03570"
     CDS             9979..>10614
                     /locus_tag="AC16YU_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6064332.1"
                     /translation="MSGPDPRRVLVVAYYFPPMGLSGVQRIVKFVKYLPRYGWRPAVL
                     TVRPGGYYAFDEELEAEVRDAGIPVHRSGSIDPNRLLGRGRPVAMPDEGRRDLLSRLS
                     QALFAPDNKIGWYPQAVRLGRSLLDHEPFDAILSSAPPYTAHLVGARLASESGLPLVL
                     DYRDDWLGNPRHVYPTHAHRALNGRMERWALRVAAGVTTINPTIAASIAKRD"
BASE COUNT         1719 a         3567 c         3684 g         1644 t
ORIGIN      
        1 gacgtcccgc ctggtcgacc gagccatccg cccgctcttc acggacggat tcttcaacga
       61 ggtgcaggtc atcatcaacg tcctctcggc ggagccggag gtcgatgcgg acgtgctggc
      121 cggcgtcggc tcctcggcgg cgctgctgct cgcgggagcg ccgttcgacg gcccgatcgc
      181 ggaggtccgc atcggccggg tggacggcgc gttcgtcgtg aacccgcaga tctcggagct
      241 cgaggagagc gatttcgacc tggtcgtcgc cggcaagagc gacgccatcg tcatggtcga
      301 gggcgagatg cgcgaggtgt ccgaggaaga catgctcgag gccctcgaca tcgcccacga
      361 ggccatccgc aagctgtgcg cggctcaggt gcagttcgtg caggaggccg gcgggccgaa
      421 gcccttctcg tacgttcccc gcgttctccc tgaagggctc ctggacaagg tccgcgggat
      481 cgtcggcgat cgcctggcga agcacgtccg cgccccgtac gagaagtcca ccttctacgg
      541 cggcatctcg gagatcaagg aggacgtcct gaccgcgctg ctgggagctg acgacccggc
      601 gtccggggcc gtccgcgcgg ctgcgaccgg cgaaggctgg acggccgagc agatcaagga
      661 ggccgtcggc acgatcgagc gcgacgtcat gcgccacatg atcctcgacg agggcaagcg
      721 catcgacggt cgcggctttg ccgatatccg cgacatctgg gcggaggtgg gttacctgcc
      781 ccgcgtgcac ggttcggcga tcttcacgcg cggcgaaacc caggtcctcg cgtccgtcac
      841 gctcggaacg tcgaaggacg tgcagccggt cgaccagatc ttcgacacga ccgacaagcg
      901 gttctacctc cactactcgt tcccgccgtt ctcgacgggc gaggcgaagt tcctgcgcgg
      961 acccggccgt cgcgagatcg gccacggcta cctggccgag cgcgccctct cgggcatgtt
     1021 gcccgattcg gaggacttcc cgtacacgat ccggctcaac gccgacgtgc tcgagtcgaa
     1081 cggctcgtcg tctatggcca gcgtgtgctc gggcgccctc gccatgatgg acgcgggcgt
     1141 accgctccgc aagccggtcg ccggcgtggc gatgggcctc atcagcgagg gcgggaagac
     1201 ggcggtgctt tcggacatcc tgggcaccga ggaccacctc ggtgacatgg acttcaaggt
     1261 cacgggcacc cgcgacggag tcacggcctg ccagatggac atcaagatct cggggctctc
     1321 gcgcgatctg ctcaagacgg ccctgatgca ggcgaacgcg ggccgcatgc acatcctgga
     1381 cgtgatggac cagaccctgg cggaaccccg cccggacctg tcgccctacg ccccgcgcct
     1441 cacgcagatc accatcgacg ccggattcat cggcgccgtg atcggccccg gcggcaagat
     1501 catccagggc atccagcgcg agacgaatac ctccatcgac atcaccgagg aggaaggagt
     1561 cggcatcgtg acgatcgccg ccacgaacga ggacgacgcg atgcgcgccc tcgagatcat
     1621 ccgcggcatc gtcaccgtcc ccgaggaggg cgaggagtac gagggcacgg tgcgaaaggt
     1681 ggaggcgttc ggcgccatca tcgagatcat gcccggcaag gaggggctcc tgcacgtctc
     1741 cgagctcgat tacggcttcg tggaccgcgt ggaggattac ctccaggtgg gcgacaaggt
     1801 gaaggtgaag ctgatcgagg ttcgcgacga cggcaagctg cgcttcagcc gcaagccgtt
     1861 cctcgagaag ccggagggct gggaagagcg tccgcgcgag cgacgtgacg gccagcgcag
     1921 cggcggcgga ggaggccgag gaggtgatcg gcgccgcggc ggcggtgacc gccgtggtgg
     1981 cggcgaccgg cgaagtggcg gcggtggagg ccgcggaggc gaccggcgcg gctgagccgc
     2041 gatccgcgac tcctgcatac aggttcgtgc cgcgttatat tcgcggcgcc ggaaggcgga
     2101 ggcttggctt ccgcctttcg tttacccccg attcccatcc gcatcctgaa gcgtgtcccc
     2161 gaggagcttt cccgagttcg aacgcacgac cctggagaac ggcgtgcggg tggtgaccga
     2221 gaccatcccg tccgtccggt ccatatcggt cggcgcctgg gtccacacgg gcagccgtga
     2281 cgaggccggc ggggagaccg gcatcagcca tttcatcgag cacatggtgt tcaagggcac
     2341 gcgccggcgc cggatgcacc agatcgccca gcgcatggag tcggtcggcg gatacctgaa
     2401 tgcgttcacc gccaaggagt acacgtgcta ctacgcgcgg gggctcgacg agcacctggc
     2461 gcgggccgtt gacacggtgt gcgacctggt gatgtcgccg tcctttcccg agcgggaggt
     2521 cgccaaggag aaggacgtcg tcgtcgagga gatgcggatg tacgaggacg ctccggagga
     2581 gctgatcttc gaccgcttcg acgcgatgct ctatccggat cacccgatgg gccggcccgt
     2641 gatcggctac gaggacaccg tccggagctt ctcccgggag cagctgctcg cgtacatggg
     2701 cgaccactac acgctggacc gcatggtcgt ggcggtcgcc ggaaacgtgt cgcatcggcg
     2761 cgtcgtgacg ctcgtccgcc aggccctgca gggcacgaac cgcgccgtat cgcacacggt
     2821 ccgggcgccc gtggccggtt accgcaccga ccggctcgtg gaacagcgga atatccagca
     2881 ggcgcacctc gtgctcggca cgcgcgggta cgacgtattc gacgatcgcc ggatcgcgct
     2941 ttccgtcctg aacacgatcg tgggaggggg gatgtccagc cggctgaatc agaacatccg
     3001 cgagcgatac ggatactgct acaacatcta ctcgttcacg aaccagtact cggacacggg
     3061 cgacttcggc gtttacatgg gcacggacgc cgggaaggtg gaccgggccg agaagctgat
     3121 cttccgggaa ctggatcgcc tggccaatac cccggtcagt ccacgcatgc tgtcgcaggc
     3181 catcagccag gtcaagggct ccatcatgct gggcctcgag tccatgggca gccggatgat
     3241 gcggcttggc aagcaggagc tgtactaccg caggtacttc acgctcgacg aggttctcga
     3301 gcaggtgagc gccgtgagca gggaggacgt gctgcgcacc gcccaggagc tgttccgtcc
     3361 ggaccagttc tcgcgcgtga tcctgatgcc ggggaatgcc gcatgaccca caaacccact
     3421 cgcaaggtcc tggtcaccgg aggggcgggg ttcatcggtt cgcatgtcgc cgacgccctc
     3481 gtggagcgcg gcttcaaggt gcacgtcctg gatgatcttt cgtccggcct tcgttccaac
     3541 gtgccggagc acgcggtact gcacgaactg gatgtccgcg atccggcggt cgcggacctg
     3601 ttccgcagcg agcggttcga gatcctcgtg catcacgccg cgcagatgga cgtccgccgc
     3661 agcgttgccg atcccgtgtt cgacgccgac gtcaaccttc gcggcctgct gaatctgatg
     3721 gaggccggcc ggacctccgg cctgaagaag gtgatcttcg cgtcgaccgg gggcgccatc
     3781 tacggtgagc cggcctacgt gcctcaggac gaggaacacc cgctgcggcc tctgtcgcct
     3841 tacggcgtga ccaagctcgc ctcggaaaag tacctgtact actacaagca cgtgcacgac
     3901 atcccgttcg tggcgctgcg ctacggaaac gtgtacggtc cgcggcagaa tccccatggc
     3961 gaagcgggag tcgtggcgat attctgcaat ctgatcctcg acggccgcca gccggtcatc
     4021 aacggagacg gtgagcagac gcgcgactac gtgttcgtct ccgacgtcgt gaaggcgaac
     4081 cttgcggcac tcgactccaa gcacacggga gtcttcaacg tgggcaccgg gcttgaaacg
     4141 tccgtgaacc gcctgttttc caggctccgg gacgaactgg agccggccgt acgggaggaa
     4201 cacggcgcgg cgaaagccgg cgagcagcga cgcagcgtcc tctcgttcga gcgtgccagg
     4261 aacctgctcg gctggcagcc cgtggtcaac gtcaaggacg gactccgcat gaccgccgcc
     4321 tggttccgcg accaccacgg cgcggacggc agctgatgtc catgtatcgc atccggctgc
     4381 agcagttcga gggccccctg gacctgctgc ttttcttcat ccggcgggat gaactggacg
     4441 tgtacgacat cccgatcgcc cggatcgcgg acgagtttct cgaatacgtg cgggttctgg
     4501 aggaagtgga cctggacggc gtcggggact tcctgtacat ggccgcgctc ctcatctcga
     4561 tcaaggccag gctgctgctg ccccaccagg agacggacga ggagggcgag cccatcgatc
     4621 cgcggcagga gctcgtcgag cggctgctcg agtacatccg gttcaaggag gcggccggcc
     4681 acctggaaag gaggttcgag aaccggcacg agcagtacac gcgcggcttc gcacccgagg
     4741 acctggtcca gaccggggag gggagcgagc agcttgtcga cgccaccgtg ttcgacctga
     4801 tcggggcact ccggcgcgtg ctcacccagg ctccggaaga gcccgagcac acggtcgttg
     4861 ccgagagctt ctcggtcgag gagcagcagc agttcctcag ggagagtctc ggcacggagc
     4921 gccgttcctt cgtcgagctc atagggggcc gcacgaaggc gttcgtcatc tcgacgttcc
     4981 tggccgttct tgaactcgcg cgcatggccg agctgcagat cttcgtggag tccggcgccg
     5041 acgattttta ccttgtcctg attccgcaca tcgaatcacc caccgatcca tgaccgaccg
     5101 tcccgaaccg gaccaggacc gggaggcctg gagcggcgtc ggctcgacac gcatcgagcg
     5161 ggtggtcgag gcgctgatct tcgcggccga cgaggcgctc ccggcccgcc gcatcgccgg
     5221 catcttcgca gaggtgaccg gcgcggagcg gccgggcgac gcggacgtgg agcacgcggt
     5281 cgatacgctg aacacccggt acgaggcgca ggatcgcccg ctgcggatcg aacggtggag
     5341 cggcggcttc cgcatggcca ccgtgccgga cgtggcccct ttcgtcaagg ccctgttcca
     5401 cagcgggcag gtgcaccgcc tttcgcgctc gctcatggag acgctggcca tcctggcata
     5461 ccggcagccc gttaccaagc ccgaggtgga ctttgtccgg ggcgtggaca gcgactacgc
     5521 cgtgcgccgg ctgctcgagc tcgggctggt cgacgtgcgg ggacgagccg attccgtggg
     5581 ccgccctctg ctgtacggaa ccacggatcg attcctcgag ctcttcggac tcaatgcgct
     5641 cggggacctt ccgaaccttc gggagatcga gtcgatcctg gatgatccgt ccttccaccg
     5701 ggagcgggcg cgtctgctga tgaaggcggg gggcctcacc ggcctcctgc cggacgacgc
     5761 cgcgccggac gcatccgagg agctgtccga tggctgaatc ctccggacga tccggccgag
     5821 gaaggaagcg tcccgcgcca ggcggcgggg actccggcac gaaacgccgg tccggtgctt
     5881 cgtcgggttc cggcgcgaag cagcgttccg gcacgcacgc ggcgcgtcgg cgcgcacgca
     5941 agggccccgc ccaggaacgc acggaggcga cgggcgcctc gaccggagcg ccgatgcggc
     6001 tgaaccggtt catcgcccgg tcgggcgtct gctcgcgccg cgaggcggac gaatggatcg
     6061 tggcggggcg ggtgcaggtg aacggcaagg tcgtcaccga gctcggtacg cgggtcgcgg
     6121 agggcgaccg cgtcgtcgtc aacggccgcg acgtgtctcc ccggcgcgcc gagtacttcc
     6181 tcctgaacaa gccgcacgac acgatcacga cgaccgagga cgaacgcggc cgccgcaccg
     6241 tcctggacct cctggaggta ccggccgagc gaaagggagc tcttttcccc gtcggacggc
     6301 tggatcgcgg cacgaccggc gtcctgctca tcacttcgga cggggagctc gcgcaccgcc
     6361 tgatgcatcc gtcgtacgag atcgacaagc tgtaccgggt gctcaccgcg gaagacgtga
     6421 cgccggagca gctggaggcg ctcgtcaccg gggttccgct ggacgacgga ccggcccgcg
     6481 cggaccgtgc cgaatacgtc gccctcccgg accggaggca gatcggcctc tcccttcacg
     6541 agggacgcaa ccggcaggtc cgcaggatgt ttgaggccct ggggcatcgc gtcgtcgacc
     6601 tcgaacgcgt gcagtacgcg ggactcacga cccggggcgt gcggtccggc cattggcgcc
     6661 gcctgaccga ccgcgaggtc acccgccttt acaaactggt caaactcaag tagtacatcc
     6721 catggagcac aaggtgttgt cggaggtcga ccggaccctc gcggccaaga tccgcgggga
     6781 gggtctcacg tacgacgacg tactactcct gccggcccgc tccaccgtga tgccacggga
     6841 ggccacgctc gctacgcgtc tcacgcggcg gatcgcgctg aacatcccgc tcgtctcggc
     6901 cgccatggac accgtcaccg agtccgacat ggccatcgcg ctggcgcgcg aaggtggggt
     6961 gggcgtgctt cacaagaaca tgtcgatcga ccggcaggcc gccgaggtgc ggcgcgtgaa
     7021 acgatccgag agcggcatga tcctggatcc gatcacgctc aagcccggcg ataccgtggc
     7081 ggacgcccgc gcgcagatgg cgcggtactc catcggcggc atcccgatcg tggacgacga
     7141 caaccggctc gtgggcatcg ccacgaaccg ggacctccgt ttccagagcg acggcacggt
     7201 gcgcctggac gtggtgatga cgcgggcgcc gctcgtgacg gccccgatgg gcacgaccct
     7261 cgaggcggcg gaagcgctgc tgcagcggca caagatcgag aagctcccgg tggtcgacga
     7321 gcaggatcgc ctcgtcggtc tcatcacctt caaggacatc cagaagaagc gccagtttcc
     7381 gctcgcctgc aaggacgagc acggcaggct ccgcgtcggc gcggccgtcg gcgtcacggc
     7441 cgacgtgctc gagcgggtct cggcgctcgt ggccgcggga gtggatttcg tcacggtcga
     7501 cacggcccac gggcactcca tgggcgtgat gcggaccatc gagcgcgtca agtcctcgtt
     7561 tgccgatctc gacgtcatcg ccggcaacgt ggccacgtcc gaagcgacgc gcgagctcat
     7621 cgaggtgggc gccgacgcgg tcaaggtggg catcggcccg ggctcgatct gcacgactcg
     7681 catcgtggcc ggagtgggcg taccccagct cacggcggtg atggactgtg cggcggtcgg
     7741 gcgcgagctg gacatcccaa tcgtcgccga cggcggcatc aagcataccg gcgacatcgc
     7801 caaggcactc gccgccggag cggagtccgt gatgatcggc ggcctcttcg cccgcgtcga
     7861 ggaaagcccg ggcgagacca tcctctacga ggggcgcaag ttcaagagct accggggaat
     7921 ggggtccatc ggagccatgg aagccggttc cagggaccgc tatttccagg acgtggagga
     7981 cgacatccgg aagctggtgc ccgagggcat cgagggacgg gttccgtact cgggaaccct
     8041 gcacgaagtg gtctaccaga tggtgggtgg tctccgcgcg gccatgggct actgcggctg
     8101 cggtgacctc gaggagtttt tcgagcgctc ccgattcgtc cgcatcacgg acgccggggt
     8161 caacgagagc cacccccaca acgtgcacat cacgaaagag gccccgaact acaccagccg
     8221 ctgaaccgtg gcctccgaaa gggactcccc tgaagggcgc atcgaggccc gtcggcgcga
     8281 gctcggcgtg gacgccctgc tcatccacga tccggccgac cttcgatggg cgtcgggatt
     8341 caccggatcg aacgggctgc tgctctgggg atccggggcg cggcacttcg tcacggacgg
     8401 ccgctacgcc gtgcaggccg ccgcggaagt cccgggagcc caggtgcacg tcccgggata
     8461 cgacctcttc ggattcctgt ccgagggcgg actggccgac gggctccgcc gcgtggcctt
     8521 cgcctcggac ctgcttcccg tcgacacgtg gaagcggctg cgctcgctcc ttcccgacat
     8581 cgactgggtg ggcgtgacgg cgctgctctc cgaggccagg gcgcacaaga ccgacgcgga
     8641 ggtcgagacg atccggcggg cgcagcgcct cgcggagcgc gtgttttcgg aagtcctggg
     8701 tctgatgcgc cccggaatga ccgagcgcga ggtcgccgcc gagctgacgg ggcgttccct
     8761 tcgcgggggt gcggactgta tggccttcga ccccatcgtg gcggtcgccg cgcgctcggc
     8821 gcttccgcac gcgcggccca ccgaccgcgc gatcgccccg ggtgatcccg ttctcgtcga
     8881 cttcgggtgc acggtgcagg ggtacgcgag cgacatgacc cgcatggtct cgttcggacc
     8941 gccctccgac gccctggtgc gggtgtacgg gatcgtgtcc gccgccctcg gggccgccga
     9001 agcgtcgatc gtcccgggcg tctcgtgccg cgacgtggac ctcgccgccc ggcgcgtgat
     9061 cgaggaggcg gggcacggcg accggttccc gcacagtctc gggcacggtg tgggcctctc
     9121 ggtccacgag tatcccgccg tttcctggag aaacgaggac ccgctgccgg agggctcggt
     9181 cgtaacgctc gagcccggca tctaccttcc cggcgcattc ggggtgcgga tcgaggatat
     9241 ggtcagggtc cttccgacgg gcccggaacg cctcaccgcg gttgatcgcg acctcgtcat
     9301 cctctgaacc atggctgatt tcatcccgta tcgccatccg ctggcctgtt ccgacgagga
     9361 ggcgcgcgac cgggcggccg ccttccggag cctcatgcag gagcgacgca cggtgcgcga
     9421 cttctcggac cgcccggttc cgcgggaggt ggtcgtcgac tgcctcgcca cggcgggctc
     9481 cgcgccctcg ggcgcgaacc ggcagccgtg gcatttcgtg gtcatcgagg acgcggacct
     9541 ccgccggcgc gtcagggagg ccgccgaggc ggaagagcgg gccttttacg aggggcgcgc
     9601 gccgtcggac tggctcgagg ccctggagcc gttcgggacc gatgcacgga agccgttcct
     9661 ggagatcgca ccggtcctca tcgccgtatt tgccgaacga tggggggagg agggaggcag
     9721 ccgggtaaag aactactacg tccaggaatc ggtgggcata gcctgcggga tgctcatcgc
     9781 ggcgctgcac caggccggac tctgcacgct gacccacacg ccgagcccca tgggctttct
     9841 ctcggacgtc ctggggcggc ccgatcgtga gaaaccgtac ctgctggtgg ttgccgggca
     9901 cggcacggag ggcgtcgccg ttccggacat cgcgcgaaag cccctcgaac agaccgtctc
     9961 gttccggtga tcgaacccgt gagcggcccc gacccgcgtc gcgtcctcgt ggtcgcgtac
    10021 tacttcccgc ccatgggcct gtcgggcgtg cagcggatcg tgaagttcgt caagtacctg
    10081 ccccgctacg gctggcggcc cgccgtcctc acggtgcgcc cgggcggata ttatgcgttc
    10141 gacgaggagc tggaggccga ggtccgggac gcggggattc ccgtgcaccg cagcggctcg
    10201 atcgatccca accggctcct gggccgcggc cggccggtcg ccatgcccga cgagggccgg
    10261 cgcgacctgc tctcccggct cagccaggcg ctgttcgccc ccgacaacaa gatcggctgg
    10321 tatccccagg cggtccggct gggccggagc ctgctcgatc acgagccgtt cgacgccatc
    10381 ctgtcgtcgg cgcccccgta cacggcccac ctcgtcggcg cacggctcgc ctccgagtcc
    10441 ggcctgccgc tggtcctcga ctaccgggac gactggctgg gcaacccccg gcacgtctac
    10501 ccgacgcatg cgcaccgggc cctgaacggg cgcatggaac ggtgggcgct gcgcgttgcc
    10561 gcgggcgtga cgaccatcaa cccgacgatc gcggcatcga tcgcgaagcg cgat
//
LOCUS       DBNHQZ010000137         5552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_987780, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000137 DBNHQZ010000000
VERSION     DBNHQZ010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_987780"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16YU_03575"
     CDS             <1..628
                     /locus_tag="AC16YU_03575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR6064333.1"
                     /translation="PEAIVPHDLCGGPPSLSTLRGYDALTVGGSGDYYVSKGNLPHFE
                     AYLDFLREVVELGHPTFASCFGFQSLVRALGGELVYDPATTEVGTFELELTGHGRIDP
                     LIGRLPARFDAQLGHIDRAATLPDGVVHLASSRLCAFQALRIPGKPIWATQFHPELDR
                     KTNLDRFRHYVANYGPLDPAERAAAEARFRESPAADALLADFLELVFS"
     gene            complement(1018..2481)
                     /locus_tag="AC16YU_03580"
     CDS             complement(1018..2481)
                     /locus_tag="AC16YU_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064334.1"
                     /translation="MKTALATFVVLGLALPMQGLAQVGPRPPVMLSLTVNHEVEVDVR
                     QGSPMVVVLGVMHAGLYSGNVEPVTIEAVGGSWANAIQLGASDETGDPVPWSLTPAHT
                     PSGSLTLDNETVGELGWWMEPTAVAAVPVGVYSLTATLDPASVQAPFAALSPARSVPV
                     VVRVTASGGALAADDRETEALRRADYFVLRGEDEAALSEVEGLLAEHPRQVGALSYRG
                     ALLRRSGRAAEALDDFAAALDALYEDTPDPEEPPTGLLRAYSEVLGEVETSTEFDVIV
                     APKTPVHPAYGVGSDQGYSIGGVEGGELWLHEDTTYTFRMRDVPAESPFYFSTSEAGG
                     GEGVYLDGVSGHPASGTSEITLTPSAATPDMLWYQSMSQPSMGWRIHVMRAGSGVDVD
                     PQTLTLPVAFHLGSAFPNPFSEETRLTLTVGRPQHIRVDVFDAEGRLIRRLSDRFMSA
                     GEPTEFVLGSSGLVTGVYFIRIEGETFRASRSVIFIR"
     gene            complement(2499..4586)
                     /locus_tag="AC16YU_03585"
     CDS             complement(2499..4586)
                     /locus_tag="AC16YU_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064335.1"
                     /translation="MTTRPISGVIILAALALGGGNDPGVKTYRTAMADAVWVGEIRDT
                     LDTGYYQEFLSGFIKYEYSYTQGPEDYYTLAGGSLTWSASGDSGGCSYSGGPTSIPIS
                     TSTSAGDMFVRSDGVYVANTYLVSSPGTVVVVCDNSSFEFDGQLQGLHIAPYIAPYFY
                     LVDSGTRMQGTYTLGAGTYTWNLTNKLPDVEIWVEDYDVWRPEAGADESTPGNAVTIR
                     ARVVDENGSPPDRPADLFRFELDGTSREPGILMNYPLPGTARDTYDLQFLPAANPDAE
                     RTAPDGQWIEMPSDFETAVTLTSFDFGAWSTVSVTATVDGIDVVGHYMGEPATVDVRL
                     PKRDEDSKIADAWKNGHGAGGLADDSDQDDQPEGDGDAGDGFTLYEEYRGFMENGSRL
                     ETSFEKKDLFIRDRLGGRVKQGIALFIAATGLEVHHELTFQEFPSDRVMNGNFSEGAH
                     VVNQHGLVIHRGPNNGASRAVGGPGLPRNVSEIQVVPSIVDEPEWLITVRTGGTTHRV
                     HRSSEDVTHEIGHAVNIWHHGERDEIVKWSVERNASGAIIRDASGFPTVYESGSGDIS
                     IYTDTDPPVRIVPLWLQGPTDTLNAWVGWSRGQHSGDATCFMGYNIANVYGVGVSTST
                     PARYLISQNGSAPSYPPKNHFCTTTIGTHYNAPSRVPFPWFGNADDAATIGPSDRGDC
                     LHRICVNDRLGAH"
     gene            5239..>5552
                     /locus_tag="AC16YU_03590"
     CDS             5239..>5552
                     /locus_tag="AC16YU_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064336.1"
                     /translation="MKRILTVAALLLATMSSLSAQDTPGIKAYSKVDFVAGSSVVLFD
                     DFLDDAIGDFPDGWDTNASAEVVTIEGTPGHWLMLSGGGVFVPVLAESLPDYFTFEFD
                     IVA"
BASE COUNT          873 a         1884 c         1837 g          958 t
ORIGIN      
        1 ccccgaggcg atcgtacccc atgacctgtg cggggggccg ccgtcgcttt cgaccctgcg
       61 cgggtacgat gcactgacgg tcgggggcag cggagactac tacgtttcga aaggcaacct
      121 gcctcacttc gaggcctacc tggacttcct gcgcgaggtg gtcgagctgg gccatccgac
      181 cttcgcatcg tgcttcggct ttcagagtct cgtgcgggcg ctcggcggcg agctcgtcta
      241 cgacccggca acgaccgagg tcggcacgtt cgagctggaa ctcaccgggc acggcaggat
      301 cgatccgctg atcggccggc ttcccgcccg attcgatgcc cagttgggac acatcgaccg
      361 ggcagccact ctgccggacg gcgtcgttca cctggcatcc agccgactgt gcgcgttcca
      421 ggccctgcgc atccccggca agcccatctg ggcgacccag ttccaccccg agctcgaccg
      481 aaagacgaat ctcgaccggt tccggcatta cgtcgcgaac tacggccccc tggacccggc
      541 cgagcgggct gccgccgagg cgcggttccg ggagagcccc gccgcggacg cactgctcgc
      601 ggactttctg gagctggtct tttcgtgacg gacgggcggt gggttgccga tggcccggtt
      661 gtcgatcccg cccacgactg acatggcccc gccttctcgg cttcacccgg ccctgcaatg
      721 gcccggagga tctcccgcgg atcggcggga cggatccggg ggtgcggccg gcacgataga
      781 tgagctcgtc cgggggggcc ggtccgcaga tgccggcgtg ctcctcgccc tcgggtacca
      841 ggatgagcgc gacgcgtccc agacggagag cgcagacgct cggggacgtg ctcggagatg
      901 ccctgatgtt cgtcgtcgca gaagcggcgc ccggatggat ggagggcgtt tcgggccgaa
      961 gccgagctcg tgatcggccc tgcggcaccg gacgttctgt cgacgtggct gaccggccta
     1021 ccggatgaag atcacgcttc gggacgcgcg gaaggtctcg ccttcgatcc ggatgaaata
     1081 gacaccggtc acgaggcccg acgatccgag gacgaactcg gtcggctcgc cggccgacat
     1141 gaatcggtcg gagagtcgcc ggatcagcct accctcggca tcgaagacgt cgacccgtat
     1201 gtgctgcggc cgcccgaccg tcagcgtcag tcgcgtctcc tcgctgaagg gattcgggaa
     1261 agcggagccc aggtggaagg cgaccgggag tgtcagtgtc tgcgggtcca cgtcgacgcc
     1321 ggacccggcg cgcatcacgt ggattcgcca gcccatgctc ggctggctca tgctctggta
     1381 ccagagcatg tcgggcgtcg cggcgctggg cgtcagggtg atttccgagg tccccgaggc
     1441 cggatgtccc gagacgccgt ccaggtagac gccctctccg cctccggctt cgctcgtcga
     1501 gaagtagaag ggtgactcgg ccggcacgtc ccgcatgcgg aacgtgtagg tggtgtcctc
     1561 gtgcagccaa agctctccgc cctccactcc gccgatggag tagccctggt ccgacccgac
     1621 gccgtacgcg gggtgcaccg gggtcttcgg ggccacgatg acgtcgaatt cggtcgaagt
     1681 ttccacttca cccaggacct cggagtacgc cctgagcagc cctgtgggtg gctcttcggg
     1741 gtcgggggtg tcctcgtaca gcgcatcgag agccgccgcg aaatcgtcca gagcctctgc
     1801 cgcgcggccc gaccgtcgga gcaacgctcc acggtagctc agcgcgccga cctgacgcgg
     1861 atgttcggcg agcagcccct ccacctcgga gagagccgcc tcgtcctcac cgcgcagcac
     1921 gaagtagtcc gcgcggcgga gtgcctccgt ctcacggtcg tccgccgcaa gggctccacc
     1981 ggatgccgtc acccggacca cgaccggcac gctcctggcc ggggagagag ccgcgaacgg
     2041 agcctgcacg gacgccgggt ccagcgtcgc ggtgagcgaa tagaccccga ccgggaccgc
     2101 ggccacggct gtcggctcca tccaccagcc gagctcgcca accgtttcgt tgtcgagcgt
     2161 cagcgatccg gagggggtgt gggcgggcgt caggctccac ggcacggggt cgccggtctc
     2221 gtcggacgct ccgagctgga tcgcgttcgc ccaggagcct ccgacggcct cgatggtgac
     2281 cggctccacg ttaccggagt agagcccggc atgcattacg cccaggacga cgaccatggg
     2341 cgaaccctgc cggacgtcga cctcgacctc gtggttcacg gtcagtgaca gcatgaccgg
     2401 cggacgtgga ccgacctggg cgagcccctg catcggcaat gcgagaccca ggacgacgaa
     2461 cgtggcaaga gcggtcttca tgtgcgggcc tccggcattc agtgggctcc gagccgatcg
     2521 ttcacgcaga tccggtgcag gcagtcgccc ctgtctgacg gaccgatagt ggcggcgtcg
     2581 tccgcgttgc cgaaccaggg gaatggcacg cgggaggggg cattgtagtg cgttccgatg
     2641 gttgtcgtgc agaagtggtt cttcggcggg tagctcggag ccgaaccgtt ctggctgatc
     2701 aggtagcgag ccggtgtcga ggtcgagacg cccacgccgt acacgttcgc gatgttgtag
     2761 cccatgaagc acgtggcgtc cccgctgtgc tgcccgcgtg accaccctac ccaggcgttc
     2821 agcgtatccg tcggcccctg cagccagagc ggcacgatcc gaacgggagg atccgtgtcc
     2881 gtgtagatgg agatgtcgcc gctgccgctc tcgtagaccg tcgggaaccc cgaggcatcc
     2941 cttatgatcg ccccgctcgc gttgcgctcg acgctccact tcacgatctc atcgcgctca
     3001 ccgtggtgcc agatgttcac cgcatgcccg atctcgtgtg tcacgtcctc ggaggatcgg
     3061 tgaaccctgt gcgtcgtccc gcccgttcgg acggtgatca gccactccgg ctcgtcgaca
     3121 atcgagggca cgacctggat ttcggagacg ttcctgggca gaccgggccc gcccaccgcc
     3181 ctgctggcac cgttgttggg tccccggtgg attaccaggc cgtgctggtt caccacgtgc
     3241 gcgccctccg agaaattgcc gttcatgacc cggtcggacg ggaattcctg gaaggtgagt
     3301 tcgtggtgga cctcgaggcc cgtggccgcg atgaatagcg cgatgccctg tttgacccgg
     3361 ccacccagtc gatccctgat gaagaggtcc ttcttctcga agctcgtctc cagtcgggat
     3421 ccgttctcca tgaacccccg gtactcctcg tacagggtga atccgtcgcc ggcgtcgccg
     3481 tcgccctcgg gctggtcgtc ctggtcgctg tcgtccgcca gtcccccggc gccgtggccg
     3541 ttcttccagg cgtccgcgat cttcgagtcc tcgtcgcgct tgggcaggcg cacgtccact
     3601 gtggccggct cacccatgta atgacccacc acatcgattc catccacggt cgccgtcacc
     3661 gagacggtgc tccatgcccc gaaatcgaac gaggtaagcg tcaccgccgt ctcgaagtcc
     3721 gacggcatct cgatccactg gccgtccggt gcagtccgtt ccgcgtcggg attggcggcg
     3781 gggaggaact ggaggtcgta cgtgtcccgt gcggtgcccg gcagcgggta gttcatgagg
     3841 atgcccggct ccctggacgt cccgtcgagt tcgaatcgga acaggtccgc ggggcgatcc
     3901 ggcggcgacc cgttctcgtc gaccacccgg gcccggatcg tgaccgcgtt gcccggggtg
     3961 gactcgtcgg cacccgcctc gggtcgccac acgtcgtagt cctccaccca gatctcgacg
     4021 tccgggagct tgttcgtgag gttccaggtg tacgttcccg ctcccagcgt gtacgtgccc
     4081 tgcatcctgg tcccggagtc cacgagatag aagtacggcg cgatgtacgg cgcgatgtgg
     4141 agtccctgga gctgcccgtc gaactcgaag gaagaattgt cgcagacgac caccaccgtg
     4201 ccgggcgaac tcaccaggta ggtgttcgcg acgtacaccc cgtccgagcg gacgaacatg
     4261 tcgccggccg acgtcgatgt cgatatcggt atcgaggtcg ggcccccgga ataggagcag
     4321 ccgcccgagt ccccggaggc actccacgtc agcgacccac ccgcgagggt gtagtagtcc
     4381 tcggggccct gtgtgtacga gtattcgtac ttgatgaagc cggacaggaa ttcctggtag
     4441 tagccggtat cgagcgtgtc ccgtatctcc cccacccaca ccgcatctgc catcgcagtg
     4501 cgataggtct ttacaccggg gtcgttgccg ccgcccagtg caagggcggc caggatgatc
     4561 acgccggaga ttggcctggt agtcatgact cgtgtctcgt ttgagtcctg tcgtggcctc
     4621 tggcgaagga gacggaagag ccagcagcgg ctcccgttgg ctcgcgcaca ggctcaagag
     4681 gccggggaat ctcgccccgt cgtggccggg gattgcacgg aggaggaacc ggggcgtgcc
     4741 gggccgatgt cggtcgcgac ggccgggccc ggcgaacgca atgccggatt gccctggtaa
     4801 gagccgggta tgctgccagc cgaagcatga cacgtcaccg cctccgggaa gatgtgcccc
     4861 gggaatacgg atgcttgcgg cgggtcaccg gacccccggg cgaggcagcc gcttccggga
     4921 atcggaagcc ggaccgcctg ggtgtacgcg gccggcaccg ccgaatgtag gtgggctccc
     4981 tacagccgac aatggggcga tagccaggtg ccacaaaaaa tattcgcgga gaatccacac
     5041 ccgcgacctt cgcccggcgt cgcgttccgg gtacctgccg accgtcgcga agggccggtc
     5101 cggacccccg cccagggacc cctactcgaa gtcggtttcc ttcgctacaa tcgaccgtcg
     5161 ggtgttgccc cccactgccc ttccgacccg tcgccccgag tccatcatcc actcgcgtca
     5221 ccaggcgtga cgaccaccat gaaacgcatc ctcacggtcg cggcgctcct gctggcgacc
     5281 atgtcctccc tgtccgccca ggacactccc ggcatcaagg cctacagcaa ggtcgatttc
     5341 gtcgcgggat cgagcgtcgt cctgttcgat gacttcctgg acgacgccat cggcgatttc
     5401 cccgacggct gggacacgaa tgcgtccgcg gaagtggtca cgatcgaggg aacgccgggg
     5461 cattggctga tgctgtccgg aggaggcgtt ttcgtccccg ttctggcgga atcgctgccc
     5521 gactacttca ccttcgaatt cgacatcgtc gc
//
LOCUS       DBNHQZ010000138         4449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1258350, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000138 DBNHQZ010000000
VERSION     DBNHQZ010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1258350"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="AC16YU_03595"
     CDS             <1..327
                     /locus_tag="AC16YU_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064337.1"
                     /translation="RSRVELPRNAAFPADEGQMRFFASRLWHDRLAFEPEAVLASSTG
                     PVLVLIGTEDPNTPMDDYVAALERGMAAAPTGDATVRVVPGRTRHTFSPAALDEVLAW
                     LARHKR"
     gene            complement(341..811)
                     /locus_tag="AC16YU_03600"
     CDS             complement(341..811)
                     /locus_tag="AC16YU_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064338.1"
                     /translation="MATVTLAASFLSGCYTYRAAEPSEVVPESRVRLTVSARQAVELE
                     EALRDTRRTFNATYVGQNNGELLFEVPILNPTPGMSSRAVNNRVSVPRSEVTGLERRE
                     LSRWRTASVIAAGVLAVSVGAWEVFGTNNDGPDEDKPPDVNNVRIPLFSFPIGR"
     gene            complement(950..2173)
                     /locus_tag="AC16YU_03605"
     CDS             complement(950..2173)
                     /locus_tag="AC16YU_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HIR6064339.1"
                     /translation="MTHWTKTTRLATLAAAVMAVTACDGLLDVDSPGRIGDDDLASAD
                     AIPGMVVGMQYDMSQAIDGGLELISMASMELFHGGSYGWAEIPRGTIEDDDSTPWSSA
                     QQARWVAEQGIVRMSEILEPNQFAQSPYAAEANVWAGFANRFLGEVFCSSVIDGGPEI
                     DHAEHFSRAEGYFTDAIALAQAAGREDLEHAAYAGRATVRAWQGNWSGAATDAEEVPI
                     GFTWWAQLDTELRNEITYETHTRFEYSVWGTLFEDHPDDPRAPWEIEYNADNSVANGA
                     NGSTPHYKQLKYTDRADDIPLAKGTEMLVLRAEAALREGASGIPAAYALMNEAREDGY
                     GMDPLTPTATLAEAWADLHYERLATNWIEGRALWDYRRWSAETGPAQNNELDGRDLCW
                     PIPESEKDANVNFRG"
     gene            complement(2185..>4449)
                     /locus_tag="AC16YU_03610"
     CDS             complement(2185..>4449)
                     /locus_tag="AC16YU_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6064340.1"
                     /translation="IWTGEIGLGASHMNSFLADTSGDPTDLYTKCGDLDNLYSLNVQA
                     STTDPDFGERVYMQDPTCPADGDWQELGPIQQYSVSVRGGAGDVTYYLSGNFNDQQGV
                     LPTQGSKDGGFRGNFGFAPTDELSFSLNSSYTRRDTRWVADGNNAVGFLLNVGRGAQN
                     YLKGGKGDDCAEVPADRVCITNGYLFEQDLLTQGDHFITGFTAEWNPMEGLSNRFTVG
                     WDFLKLKNETTRPFGNLFTPDGYFWDENTTNTKLSLDYAGSFQNSFGDDVASTFSWGG
                     QIFRDYRRWTELDVGTFAGPGRPTIDSGAEVTYRNENQVSQTSAGFFLQEMLGWKDLL
                     FVTAGLRVDGNSAFGENFGLQMYPKLSLSYILSDYDFWPSDAVETFKLRAAIGESGKA
                     PPPFARVRTWSAANIEGLAGFTPGDPGNADVGPERTRELEVGFDASAIQGRLGVEATY
                     FTARTTDALIGVDLPASQGFLSNRTTNVGELQNSGFEFQITTGLLRTETFDWRVRTNM
                     SFLRSEAVDLDGDPEFDTCIYVGLLSEICEGERFPVLVGDMVTNPDAFADPVIVEDTT
                     IGPTYPTRMIGIGTTLSFGNSLTVDALLEHQGGHYLTNYTGYQNGRRGAWYPCFEIQG
                     QMIEAYEGNASALDGVTAQERARCAISSTVNPGYGFSSDYWMEKADFWKLRQITLSYR
                     LPEGLLAFARSATVNLSARNLLTWTDYSGTDPEVQDFADQTGTTVGGGEFGRRDYYQV
                     PNPRTYMLSVRLSW"
BASE COUNT          690 a         1467 c         1472 g          820 t
ORIGIN      
        1 cgctcccgcg tggagctacc gaggaatgcg gcgttccccg ccgacgaggg gcagatgcgc
       61 ttcttcgcga gtcgcctctg gcacgaccgg ctggcgttcg agccggaggc ggtgctcgcg
      121 tcgtcgaccg ggcccgtgct cgtgctcatc gggacggagg accccaacac cccgatggac
      181 gactacgtgg cggctctgga gcgcggcatg gcggcggcgc ccacgggcga cgccaccgtg
      241 cgcgtggtac ccggaaggac ccgccacacc ttctcgcccg cggcgttgga cgaagtcctc
      301 gcctggttgg cgaggcacaa gaggtaggcg gtccgctccg ctacctgccg atcgggaagg
      361 agaacagcgg gatgcggacg ttgttcacat ccggcggctt gtcctcgtcg ggaccgtcgt
      421 tgttcgtgcc gaagacctcc caggcgccaa cgctcacggc caggacgccg gctgcgatca
      481 ccgacgccgt tcgccacctg gacagctccc ggcgctccag gccggtgacc tcgcttcgcg
      541 gtaccgacac acggttgttt acggccctcg aggacattcc gggtgtgggg ttcaggatag
      601 gcacctcgaa gagcagctcg ccgttgttct ggcccacgta cgtggcgttg aaggtgcggc
      661 gcgtgtcgcg tagcgcctcc tcgagttcca cggcttggcg tgccgatacg gtcagcctca
      721 cgcgactctc cggaacgact tcggacggct cggcagcccg gtacgtgtaa cagccggaga
      781 ggaacgacgc tgccaaggtg acggtcgcca tggcgtggat cggtctaagt cccatcggcc
      841 cccctttcgt ctgcgatcgt acgttcaggc gttcccaaat ctaaagagaa agcaggggcg
      901 gccgaagccg cccctgccgt cttttctgag gtgtgacggg tggtgtgcgt cagcctctga
      961 agttcacgtt cgcgtccttc tccgactccg gaatgggcca gcagagatcg cgtccgtcca
     1021 actcgttatt ctgcgcaggc ccggtttccg ccgaccagcg gcggtagtcc cacagagcgc
     1081 ggccctcgat ccagttcgtg gccaaccgct cgtagtggag atcggcccac gcttcggcga
     1141 gagtcgccgt cggcgtcagt ggatccatcc cgtagccatc ctcacgcgcc tcgttcatga
     1201 gggcgtacgc ggccgggatt cccgacgcac cctcacgcag cgcggcttcg gcccggagca
     1261 ccaacatctc ggtccccttg gccaacggga tgtcgtccgc gcggtccgtg tacttcagct
     1321 gcttgtagtg gggcgtcgag ccgttggcgc cgttggctac gctgttgtcg gcgttgtatt
     1381 cgatctccca cggcgctcgt ggatcgtccg ggtggtcctc gaagagcgtg ccccacaccg
     1441 agtactcgaa acgcgtgtgg gtctcgtacg tgatctcgtt gcgcagctcc gtgtcgagct
     1501 gcgcccacca tgtgaagccg atgggcacct cttccgcgtc cgtcgcggcg ccgctccagt
     1561 tgccctgcca agcgcgcacg gtcgcgcgtc cggcgtacgc cgcatgctcg aggtcttcgc
     1621 gacctgcggc ctgtgcgagg gcgatggcgt cggtgaagta tccctccgcc cggctgaaat
     1681 gctcggcgtg gtcgatctcg ggcccgccgt cgatgacgct cgagcagaag acctcaccca
     1741 ggaaccggtt ggcgaaccct gcccagacgt tcgcttccgc cgcgtatggg ctctgagcga
     1801 attgattcgg ctccaggatc tccgacatac gtacgattcc ctgctccgcc acccaccgtg
     1861 cctgctgggc ggagctccat ggagtggaat cgtcgtcctc gatcgtccct cgcgggatct
     1921 cggcccagcc gtagctgccg ccatgaaaga gctccatgct ggccatggag atgagctcga
     1981 ggccgccgtc gatggcctgg ctcatgtcgt actgcatccc caccaccatg ccgggaatgg
     2041 catcggccga tgccaggtcg tcgtccccga tccgtccggg gctgtccacg tcaaggagac
     2101 cgtcgcaagc ggtgaccgcc attacggcag cagccagcgt cgccagtctc gtcgttttgg
     2161 tccagtgcgt catttctcgc ctcctcacca gctgaggcgg acgctgagca tatacgtccg
     2221 cgggttgggg acctggtagt agtcgcgtcg gccgaactcg ccaccgccga ccgtcgtacc
     2281 ggtctggtcg gcgaagtcct gaacctcggg gtcggtgccc gagtagtcgg tccaggtgag
     2341 caggttgcgc gccgagaggt tgaccgtcgc gctccgggcg aaggccagga gaccttcggg
     2401 cagccggtag ctcagcgtga tctgacgcag cttccagaag tcggctttct ccatccagta
     2461 gtcggagctg aagccgtacc ccgggttcac cgtgctgctg atggcgcagc gggcgcgctc
     2521 ctgggccgtg accccgtcca gtgccgacgc gttaccctcg tacgcctcga tcatctgccc
     2581 ctggatctcg aaacaggggt accaggcgcc tcggcggccg ttctggtatc cggtgtagtt
     2641 ggtgaggtag tgcccaccct ggtgctcgag cagcgcgtcg acggtcaggc tgttgccgaa
     2701 gctcagcgtc gttccgatgc cgatcatgcg cgtcgggtag gtgggtccga tggtcgtgtc
     2761 ctccacgatc accgggtcag cgaacgcgtc cgggttcgtg accatgtcgc cgaccaggac
     2821 cgggaaccgc tcaccctcgc agatctcgga cagcagaccc acgtagatgc aggtgtcgaa
     2881 ttccgggtcg ccgtcgaggt cgacggcctc ggacctgagg aacgacatat tcgtgcggac
     2941 ccgccagtcg aaggtctccg tccgcagcag ccccgtggtg atctggaact cgaagcccga
     3001 gttctgcaac tcacccacgt tcgtcgtgcg gttggacagg aagccctgag aggcagggag
     3061 gtctacgccg atgagagcgt cggtggtgcg ggccgtgaag tacgtggcct ccacgccgag
     3121 acgaccctgg atcgccgagg cgtcgaatcc cacctcgagc tctcgcgtac gctcgggacc
     3181 gacatcggcg ttgccggggt cacccggcgt gaagccggcg agtccctcga tgttggcggc
     3241 cgaccacgtc cgcacgcggg cgaacggcgg cggcgctttg ccggactcac cgatggctgc
     3301 ccgcagcttg aaggtctcga ccgcgtccga gggccagaag tcgtagtcgg acaggatgta
     3361 cgacaggctc agcttcgggt acatctggag cccgaagttt tcaccgaagg cgctgttacc
     3421 gtccacccgg agcccggcgg tgacgaagag caggtccttc caccccagca tctcctggag
     3481 gaagaacccg gcactggtct ggctgacctg gttctcgttc cggtaggtga cctccgcgcc
     3541 cgagtcgatg gtcggtcgcc cggggccggc gaaggtgccg acgtccagct ccgtccagcg
     3601 acggtagtca cggaagatct gtccgcccca cgagaaggtg gaggccacgt cgtcgccgaa
     3661 gctgttctgg aacgaaccgg cgtagtccag cgacagcttg gtgttcgtcg tgttctcgtc
     3721 ccagaagtaa ccatcgggcg tgaacagatt cccgaagggc cgggtcgttt cgttcttgag
     3781 cttcaggaag tcccagccga cggtgaaccg gttggacagc ccttccatcg gattccactc
     3841 ggccgtgaag ccggtgatga agtggtcgcc ctgcgtgagc aggtcctgct cgaacaggta
     3901 gccgttggtg atgcagaccc ggtcggcggg tacctcggcg cagtcgtcgc ccttgccacc
     3961 cttcaggtag ttctgggcgc ctcggccgac gttgagcagg aagccgacgg cgttgttgcc
     4021 gtccgcgacc caacgggtgt cacgccgggt gtaggagctg ttcagcgaga agctgagctc
     4081 gtcggtgggc gcgaaaccga agtttccgcg gaagccgccg tccttggagc cctgcgtcgg
     4141 gaggacgccc tgctggtcgt tgaagttgcc cgacaggtag tacgtcacgt cacccgcgcc
     4201 gccgcgcacg gacaccgagt actgctgaat cgggccgagc tcctgccagt cgccgtcggc
     4261 cgggcaggtc ggatcctgca tgtagacccg ctcgccgaaa tccggatcgg tcgtgctcgc
     4321 ctggacgttc agcgaataga ggttgtccag gtcgccacac ttcgtgtaga gatccgtcgg
     4381 gtccccgctg gtgtccgcca gaaagctgtt catgtggctg gcgcccagac cgatctcacc
     4441 tgtccagat
//
LOCUS       DBNHQZ010000139         6158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_309027, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000139 DBNHQZ010000000
VERSION     DBNHQZ010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_309027"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="AC16YU_03615"
     CDS             complement(<1..854)
                     /locus_tag="AC16YU_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064341.1"
                     /translation="MKQLFKRVAIALGVLVGLVIVAGAVLYALGGGRLGAHFDIRTES
                     VTITSDSASIARGRHLAESVMLCHGCHGDDLGGGVLVDEPLLATIHASNLTAGRGGIG
                     QTYSDADFVRAIRHGVNPEGRALMIMHSDAYHNLRRSDLGALLAHVRSMPPVDREVPG
                     TSVGVLGRILVALGQFDREAMPLLPAEVIDHEAPLAERPEAAVTPEYGEYLVSLALCR
                     MCHGPLLRGGLPIDEGAPPGPDITVYGRDEVWSAQHFLTTIRTGVTPYGRTLDTEYMP
                     WEAYRGMT"
     gene            complement(1118..1477)
                     /locus_tag="AC16YU_03620"
     CDS             complement(1118..1477)
                     /locus_tag="AC16YU_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6064342.1"
                     /translation="MTPRHLVQLWVEAFNRADADALAAFYSEGATNHQVAESPVVGRE
                     AIREMFRAAFATAAMTCLVENLFEDGEWAILEWRDPLGLRGCGFFHVVEGKIVFQRGY
                     WDKLSFLRMHGLSPSDA"
     gene            complement(1590..2138)
                     /locus_tag="AC16YU_03625"
     CDS             complement(1590..2138)
                     /locus_tag="AC16YU_03625"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6064343.1"
                     /translation="MRVLLPVLAAFVLVALSACTSDAPAPSVPEISEAEEALARAKTE
                     QLNKRHVRRFFEEVWSTGDLALRDSFLAPGYQGHVAGSPGPIDRETWTAWLTGFREAF
                     PDARFTVDDMLADGDLVAARLTMRGTHLGELNGIPPTGREVTVTGMSIERVADGRIVE
                     GWNQNDALGLLTQLGVLPQPGQ"
     gene            complement(2340..2630)
                     /locus_tag="AC16YU_03630"
     CDS             complement(2340..2630)
                     /locus_tag="AC16YU_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_171099932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR6064344.1"
                     /translation="MEDRELYREKAQARLDELKADLDKLKARAAGDSAEARLELNEKI
                     RGLESRLSEGKSRLAELMDAGDDAWDSVKEGFESAWGSLRSAFGEAKDRFTK"
     gene            complement(2721..3107)
                     /locus_tag="AC16YU_03635"
     CDS             complement(2721..3107)
                     /locus_tag="AC16YU_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR6064345.1"
                     /translation="MAIRVVRLGSERAPDEGLRIGTVRRPPRGVPRERYSTENWYDAW
                     LPQLSPGAELVKRAQAAGTEREWKGFVSRYRAEMSSPDNARLLALLAALSKTVNLSVG
                     CYCENEARCHRSVLRDLLAEAGAEIL"
     gene            complement(3368..3622)
                     /locus_tag="AC16YU_03640"
     CDS             complement(3368..3622)
                     /locus_tag="AC16YU_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR6064346.1"
                     /translation="MNWIISLIIGGIVGWLASLVMKADAQMGLVANVVIGIIGSMLGY
                     WLAGMLGIAPTGGILRFVVAVAGAALLIWILRAVGAFKKA"
     gene            complement(3689..4084)
                     /locus_tag="AC16YU_03645"
     CDS             complement(3689..4084)
                     /locus_tag="AC16YU_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HIR6064347.1"
                     /translation="MGFFSKILEKLGIGGQPEAPGPVRVATPETHGPIPFAPAAPEPI
                     AVVDVVAQLEKRAAANPQELNWRTSIVDLLKLLDIDSSYAARKELATELGCPPDKMAD
                     SAQMNMWLHQAVLARVAANGGNVPRDLLD"
     gene            complement(4285..4632)
                     /locus_tag="AC16YU_03650"
     CDS             complement(4285..4632)
                     /locus_tag="AC16YU_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6064348.1"
                     /translation="MPAPIVFFDIAGPDVGALRDFYSRVFGWSIDGTGRFVVQVSSPI
                     GAALREDPAQKRLYIGVEDVVATLLDVARNGGAVEAPRFEVPGVAVLGLFRDPAGNPM
                     GLVELEAGEPKVP"
     gene            complement(4670..5167)
                     /locus_tag="AC16YU_03655"
     CDS             complement(4670..5167)
                     /locus_tag="AC16YU_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010375833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064349.1"
                     /translation="MNPRPLLPLLALLSTLVARPASAAPCELPAHRQFDFWLGEWDVR
                     TPDGKLAGTNRIEREYDGCVVHERYVTGRGYGGESLNVYDASRGVWHQTWVDSSGMLL
                     LLEGTFRDGSMVLEGETVAADGQVARQRITWTPGEDGSVRQHWQATDAEGGWITVFDG
                     RYTRR"
     gene            complement(5330..>6158)
                     /locus_tag="AC16YU_03660"
     CDS             complement(5330..>6158)
                     /locus_tag="AC16YU_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6064350.1"
                     /translation="NHPAERTRLMKRWPALLGSLIVASCVSTPPVPGDVSRQLAPSGE
                     LRAAVNHGNPVLATKDAATGELRGVAVDLSRELGRRLRVPVTHVGYGTVADLLAGLDA
                     GEWDVAFLAVDPARRGAIDFTSAYMEVENTYLVPGQSDLRHADEVDRPGMRIAVQEGN
                     AADLFLTRQLRHASLLRSPDEAGALALVKDGSADARASGRHLLLLEAAADPAYRVVEG
                     RFSDIPHAIGVPSGHRAAAEYLDCVIEELKRSGFIRRMVDSSGNRGVAVAPPVGGAC"
BASE COUNT          971 a         2102 c         2120 g          965 t
ORIGIN      
        1 tcggtcatcc cgcgataggc ttcccagggc atgtattcgg tgtcgagcgt ccggccgtac
       61 ggggtaacac cggtccggat ggtcgtgagg aaatgctgcg ccgaccatac ttcgtcgcgc
      121 ccatagaccg taatgtcggg tcccgggggt gctccctcat cgatcgggag gcctcccctg
      181 agcaagggcc cgtgacacat cctgcacagg gccagcgaga cgaggtattc tccatactcc
      241 ggggtcacgg ccgcttcggg ccgctcggcg agaggggcct cgtggtcgat gacctcggcc
      301 ggaagcaggg gcatcgcctc gcggtcgaac tggcccagtg caaccaggat gcgccccaga
      361 acaccgacag acgtgccggg gacctccctg tcgacgggtg gcatcgaccg gacgtgagcg
      421 aggagcgcgc ccaggtcgga ccgccgcagg ttgtggtatg cgtccgagtg catgatcatg
      481 agtgcgcggc cttcagggtt cacgccatgc cggatggcac gcacaaagtc cgcgtcactg
      541 tacgtctgac cgataccgcc ccggccggcg gtcaggttgg acgcatgaat ggttgcgagg
      601 agaggctcgt cgacgaggac gcctccgccc aggtcgtcac cgtgacaccc gtgacagagc
      661 atcactgact ccgcaaggtg acgaccccgg gcaatggacg ccgagtcgga ggttatcgtg
      721 acggattccg tccggatatc gaagtgcgca ccgagtcgac ctccgccaag ggcgtacagg
      781 acggcgcccg cgacgatcac cagcccgacc aggacgccga gcgcaatcgc cacccgcttg
      841 aacagttgct tcatggttcc ggccgctggg gatactgcgg tccgactcgc cccggcaggc
      901 ctcgcgccgg tccccggcac ggtctgccga cggcggacgg gggtagctcg cccgaatata
      961 gcggctcgac attcacatgc aaccgggccg gaatcgacgc ggccccacgg aagcggcgag
     1021 cgtcggacag ccttcggtcc ggtctctcac ggactgcagt tcgatccgcg tggcggccac
     1081 ttccggctat ctggcggcgt gctcggtaga cgccgcgtca tgcatcagac ggcgaaagcc
     1141 cgtgcatccg caggaacgag agcttgtccc agtatccccg ctggaagacg atcttccctt
     1201 ccaccacgtg gaagaagccg cagcccctca ggcccagcgg atcgcgccac tcaaggatgg
     1261 cccattcgcc gtcctcgaac aggttctcca cgaggcacgt catggcggcc gtcgcgaagg
     1321 cggcccggaa catctcgcgg atggcctcgc gacccacgac gggcgactcc gccacctggt
     1381 gattggtggc gccctcggag tagaaggcgg cgagcgcgtc ggcgtcggcg cggttgaacg
     1441 cctcgaccca gagctggacg agatggcgtg gagtcatggg atggaggccg cgtctgtagc
     1501 ttcgaggcgt cggggccggt cggcggggag gccgcgcgca cacgcgcggc gagcgtgctg
     1561 ccgagagacg acgatcgtcg tccggcggtt tactgtccgg gctgcgggag cacaccgagc
     1621 tgggtcagga gacccagcgc atcgttctga ttccagcctt cgacgatccg gccgtccgcc
     1681 acgcgctcga tgctcatccc ggttacggtc acttcgcgcc cggttggcgg aatgccgttc
     1741 agctccccga ggtgcgtgcc gcgcatcgtg aggcgtgcgg ccacgaggtc accgtcggcc
     1801 agcatgtcgt cgaccgtgaa ccgggcgtcg ggaaaggcct cgcggaagcc cgtgagccag
     1861 gccgtccagg tctcccggtc gatcgggccg gggctccccg ccacgtgtcc ctggtagccc
     1921 ggggcgagga aggaatcccg aagcgcgagg tcgcccgtgc tccagacctc ctcgaaaaac
     1981 cgccggacgt gccgcttgtt cagctgctcg gtcttggccc gcgccaaggc ctcctcggcc
     2041 tccgatatct ccgggacgga aggggccggt gcgtcggaag tgcaggcgga aagtgcgacc
     2101 agcacgaaag cggcgaggac gggaaggagg acgcgcatgg acaaggcggg aggcttgcga
     2161 aggaaatcac ttgtgccgcc cccgaactcc ggcgggcatc ggacgagctc tccggtgccg
     2221 cgacgcaggg gcagaaggca cgaatataat ggccgcgccc cgacgccggc gcgtccatgc
     2281 ccgccgcagg gagtccacgg cggtccggaa tacattcgcg gtagtgcgcc cggccgcggc
     2341 tatttcgtga accggtcctt cgcttccccg aacgccgacc gcagggagcc ccaggcggac
     2401 tcgaatccct ccttgacgga gtcccaggcg tcgtcgccgg catccatgag ctcggcgagc
     2461 ctggacttgc cctcggacag cctggactcg aggccccgaa tcttctcgtt cagttcgagc
     2521 cgggcctcgg cgctgtcgcc ggccgccctg gccttcagct tgtcgagatc ggccttcaat
     2581 tcatccagcc tggcctgggc cttttccctg tacagttccc tgtcttccat ggtgattgcc
     2641 tcgcgtcggg ggtggaatgc tcccgcagta tagaccacga ccgggtcgcg ggcgagaaat
     2701 cgacgggtgc ggttccgtgg ttaaaggatc tcggctcccg cctcggcaag gaggtcgcgc
     2761 aataccgagc ggtggcagcg cgcctcgttc tcgcagtagc agccgacgga gaggttgacg
     2821 gttttcgaga gcgccgcgag cagcgcgagc agtctcgcgt tgtcgggcga ggacatttcg
     2881 gcgcggtacc gggagacgaa gcccttccac tcgcgctccg tgccggccgc ctgggcccgt
     2941 ttcacgagtt cggcgccagg cgagagctgg ggcagccagg cgtcatacca gttctccgtg
     3001 gagtaacgct ccctgggcac gccgcgcgga ggcctgcgga ccgtgcctat gcggagcccc
     3061 tcgtcgggcg cccgctccga gcccagcctg actacccgga tcgccatgag cgttgaagta
     3121 attcagtcct ccgactcgcc ggcagacgcg tcgttccggc tgggggcccc gatgaccccg
     3181 gtccgggcgc aggcagcccc gctgaccgcg aatgagcgcc gagggcacac aaaacctctc
     3241 tgccgccgcg tgcgcgcgag gcagtgaggt cttccatctg tccgcgtgaa agacgaagtg
     3301 ccgtccgcca ggctaccggg gggatgcccg gccggcacct cgatcattcg ccgcggatcg
     3361 ccgggcgtca cgccttcttg aaggcgccca ctgcccgcag gatccagatc agcagggccg
     3421 cgcccgccac cgccacgacg aagcgcagga tgccgccggt gggtgcgatg ccgagcattc
     3481 cggccagcca gtacccgagc atcgatccga tgatgccgat cacgacgttg gcgaccagac
     3541 ccatctgggc atcggccttc atcacgaggc tcgcgagcca gccgacgatc ccgccgatga
     3601 tcagggaaat gatccagttc atgtccgtgt accacttcta atggaggccg cccgcccgcg
     3661 cgggcggccg aatcggtgcc tgccggactc agtcgagcag gtccctgggc acgttgccgc
     3721 cgttggccgc gacgcgggcc agcacggcct ggtgcagcca catgttcatc tgggccgaat
     3781 cggccatctt gtccgggggg cacccgagct ccgtggccag ttccttgcgt gcggcataac
     3841 tgctgtcgat gtcgaggagc ttgaggaggt ccacgatgga cgttcgccag ttgagctcct
     3901 gggggttcgc cgcggcgcgc ttctccagct gcgcgacgac atccacgacg gcaatgggtt
     3961 cgggtgcggc cggcgcgaac ggtatcgggc cgtgggtctc gggtgtggcg actcgaacag
     4021 gcccgggggc ctcgggctgg ccgccgatgc cgagcttttc gaggatcttg ctgaagaatc
     4081 ccatggtgca ctccttgctt gccggcgagt ccggtcggcc cctcggaatg gagcgacggg
     4141 tctcctccgg ccatgaacag gcagggatgg gtaccgataa gggggcgttt ggttgccctg
     4201 tcctgcggat ccgtcagtcc gcgcggcgca cctggcagag gtgtgcgcga tgcggtgggt
     4261 ggctaccgat cccgggccgc catgctaagg caccttcggc tcacccgcct cgagctccac
     4321 gagccccatg ggattcccgg cgggatccct gaagagcccc agtacggcca ccccgggcac
     4381 ttcgaaccgc ggggcctcca ccgcgccacc gttccgcgcc acgtccagca gcgtggccac
     4441 cacgtcctcg acgccgatgt acagccgctt ctgcgccggg tcctctcgga gcgcggcccc
     4501 gatcggcgaa ctcacctgga cgacgaaccg ccccgtcccg tcgatgctcc atccgaacac
     4561 gcgggagtag aaatcccgca gggcgccgac gtccggtccg gcgatgtcga agaacacgat
     4621 cggtgcgggc atggtcgggg gctgtcgcgg ttggccggat tccgagggct caccggcggg
     4681 tgtagcgccc gtcgaacacg gtgatccacc cgccctcggc gtcggtcgcc tgccagtgct
     4741 ggcggacgct gccgtcctca cccggcgtcc aggttatccg ctgccgggcg acctggccat
     4801 cggcggcgac cgtctcgccc tccagcacca tgctgccgtc ccggaaggtg ccctcgagca
     4861 ggagcagcat gccggaactg tccacccagg tctggtgcca gaccccgcgc gaggcatcgt
     4921 agacgttcag gctctcgccg ccgtagccac ggccggtcac ataccgctca tgcacgacgc
     4981 agccgtcgta ctcgcgctct atgcgattgg tgccggcaag cttgccgtcc ggagtgcgca
     5041 cgtcccactc gccgagccag aaatcgaatt gccggtgggc cggcaattcg cacggagcgg
     5101 ccgaggccgg ccgggcgacc agggtcgaga gcagcgcgag gagcggcagg aggggcctgg
     5161 ggttcattcc gtggctcgaa cgagcggtga tcgggggtgg caggatctca ggacgccgcc
     5221 tcgaatattg acggcgtgtc gtgcattgtc gcgagtcgcg acggtgaacg gtggttcgat
     5281 cggtccagga tggcggattc cgccggcgag cggcccgtcc ggcctgcggt cagcatgcgc
     5341 cacccaccgg cggcgcgact gcaacgccac gattcccgga cgaatcgacc atgcgccgga
     5401 tgaaccccga gcgcttcagt tcctcgatca cgcaatccag gtactcggcc gcggcgcggt
     5461 ggccggaagg cactccgatt gcgtggggga tatccgagaa tcggccctcg accacgcggt
     5521 aagccgggtc ggccgccgct tccaggagca gcaggtggcg gccgctcgcg cgggcgtccg
     5581 cggagccgtc cttcacgagg gcgagggcgc cggcttcgtc ggggctgcgc agcagggagg
     5641 cgtgcctcag ctgccgggtg aggaagaggt cggctgcgtt gccctcctgt acggcgatgc
     5701 gcatgccggg gcgatccacc tcgtccgcgt gccggagatc cgattgcccg ggcacgaggt
     5761 acgtgttttc gacctccatg taggcggaag tgaaatcgat tgctccgcgc cgggcggggt
     5821 ccaccgccag gaatgcgacg tcccattcac cggcatccag cccggcgagc aggtccgcga
     5881 ccgtcccgta gccgacgtgt gtcacgggca cacgaagccg ccgcccgagt tcccggctca
     5941 ggtcaacggc gacgccgcga agctctcccg tcgcggcgtc cttcgtggcc agcacgggat
     6001 tcccgtggtt gacggctgcc cgaagctcac ccgacggcgc gagttgacgg ctcacgtcgc
     6061 ccgggaccgg gggcgtcgac acgcaggatg ccacgatcag cgaccccaga agtgcgggcc
     6121 atcgcttcat cagtctcgtc cgctccgccg gatgattg
//
LOCUS       DBNHQZ010000140         5098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3561713, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000140 DBNHQZ010000000
VERSION     DBNHQZ010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3561713"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC16YU_03665"
     CDS             <1..629
                     /locus_tag="AC16YU_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064351.1"
                     /translation="VIGGSLQTSASGQGSVTLTSANPLPADGIALVTATTADKDQNDV
                     VGYAPVIFSGLPVLTLTPGTLALGQTYLLTVTDYNGNPLVAGTSISVRVEGTAVKGVG
                     TTNVTLDDSAFLGGFDYQHVVRGPGITEFTFAAVADVDPDATNPEEPVLEAITISVSG
                     PNGRIEFVLTAGTAGKDGPGGVVVNTEGAEARTLPDGRLEVRLTEANR"
     gene            879..3638
                     /locus_tag="AC16YU_03670"
     CDS             879..3638
                     /locus_tag="AC16YU_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6064352.1"
                     /translation="MSRTIHCFARSGMFALALLLAGSASTLAQVRVTGVVTDVDTGEP
                     LPGANVVVLGTTLGSATDVEGRYTIAGVPAGTHTLEASFVGFETLRRVVAVRGTETRV
                     DFTLTFSNQAMEALEVFASRAVERRTPVAYSNVDKVQVQRELGSRDVPMVLNTTPSVY
                     ASQSGGGAGDARINVRGFNQRNVAIMINGVPVNDMENGWVYWSNWDGVGDATSSIQLQ
                     RGLSAVNLATPSIGGTMNVLTDPASIGRMARIKQEVGNDGFLKTSVFANTGLMGNKFA
                     VSLAGVRKTGDGYYDGTWTDAWAYYGAVSYQVNAKHRLDLFAIGAPQRHGQNLYLQNL
                     MAYDADFAAEVYEKDGLGQSTIEGAQAYFWDDKNYKGRRWNENVSAVSSDYTLEQHNG
                     FGIVERPETCTIAGEEVSCINERENFFHKPQINLNWYAQFTEKLLLSTVAYYSGGKGG
                     GTGTSGSMVWDYTGPSRVVNYDATIARNETNATGSRGILRNSHNVQDTWGAITKLKIN
                     VNEALTAEVGLDWRTAEIRHYRTVRDLLGGDYYLREDSDFWDGPQELALGDVFDYDNT
                     NTVDWFGGFAQAEYVKDNLSLYGMTGVSTIKYTFVDDFVDAGDGTPRTVETDNIYGYQ
                     VKGGGLYNVGEELDVYANAGWVSKVPIFDGVIDDVTGTLNTDPQNEEFVSVEAGVMYR
                     SRTMPLSVAANVYRTTWKNRTIARDVTLAGGTEGIVNLLGLDALHQGVEVEAAYQPLP
                     FLRFDGVASLGDWTYTEDVSARYVPDGREPNLVEQLRLYTRDLKVGDAPQTQFVLGVS
                     VFPVDGLYLKLLGRYYTDFYAEFDPLSRTVEGDRTQSWQIPEYTVFDLHAGYNVPITS
                     NRFDVAIFANVFNVFDETYVLEAIDNSSFNAYTANGVNHRADDAEVFLGLPTTFNLGL
                     TVTFR"
     gene            complement(3788..4174)
                     /locus_tag="AC16YU_03675"
     CDS             complement(3788..4174)
                     /locus_tag="AC16YU_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064353.1"
                     /translation="MFVSLLVVTLALAAICSFVIARIFRGPIDRTLDRIVGAEFGAAW
                     KRYLTFAIYVVGISGGVRVWDLEKYITPAPDNPIVLNSDRWILELYRTLIGTMQGVAW
                     MLLVFFAFALVAYVIVRRGERPAAGA"
     gene            complement(4256..>5098)
                     /locus_tag="AC16YU_03680"
     CDS             complement(4256..>5098)
                     /locus_tag="AC16YU_03680"
                     /inference="COORDINATES: protein motif:HMM:NF017142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR6064354.1"
                     /translation="REQYTTQMWIFEGITDYYADLALLRGGLISEEAFLGGLAGTVRA
                     FDLDPGRRVTSVAMSSFDSWAKQDDPPPFTFYSFYTAGKALGLVLDLEVRGRTGGEKS
                     LDDVMRHLYDTYYRNGEGVPEDGFQRALEAVTGTTFQAFFAAHVDSTAAVDWDAALVH
                     AGLRLARRPDERRPVRVGVELDGLRVTSVRPGSGEFRAGLDVDDVIVAVDGSPADGES
                     GFSLDGRAPGDEVELTVERWGEQRTVRLVLDASDPVYAFEPSEGPTDLQRRIRTAWLA
                     AGGR"
BASE COUNT          851 a         1819 c         1678 g          750 t
ORIGIN      
        1 gcgtcatcgg gggctcgctg cagacgagcg cctcgggcca ggggtccgtc acgctgacct
       61 cggccaaccc gcttcccgcg gacggcatcg cgctcgtcac ggcgacgacg gcggacaagg
      121 accagaacga cgtcgtcggc tacgcgccgg tgatcttctc gggccttccg gtcctgaccc
      181 tgacgcccgg cacgctcgcg ctcggccaga cctacctcct cacggtcacc gactacaacg
      241 gcaacccgct cgtcgccggg acgtcgatct cggttcgcgt ggagggcacg gccgtcaagg
      301 gcgtcggcac gaccaacgtg accctcgacg actcggcctt cctgggcggc ttcgactacc
      361 agcacgtggt gcggggaccg gggatcaccg agttcacgtt cgcggccgtg gcggacgtcg
      421 acccggacgc caccaacccc gaggagcccg tgctcgaggc gatcacgatc tcggtctccg
      481 gtccgaacgg ccggatcgag ttcgtgctca ccgccggaac ggccggcaag gacggcccgg
      541 gcggcgtggt ggtgaacacc gagggcgccg aggccaggac gcttcccgac ggacggctcg
      601 aggtcaggct gaccgaggcc aaccggtaga cccgggcggc gccgcgcgtc gccgcggaga
      661 cttcgccagg ggcggtgccc gtcatcccgg ttccggggac gcggcaccgc cccttcgcgt
      721 ttcccccggt gcacagttca cggttcggtg ctcccttctt ccgcgaacgt tcacttcggg
      781 attttcgtcc tccaatacat ttggcccgcc tgaagccccc ggccccgggc cctacgggct
      841 gaacccttcc caacacccac ccgcatttcc acaaagccat gagcagaacg atccactgct
      901 ttgcccgctc cgggatgttc gccctcgcgc tgctgctcgc cggcagcgcc tcgacgctcg
      961 cccaggtgcg cgtcaccggc gtcgtcacgg acgtcgacac gggagaaccg ctccccggtg
     1021 ccaacgtcgt cgtgctgggc accacgctgg gctccgccac cgacgtcgag ggacgctaca
     1081 cgatcgccgg cgtcccggcc ggcacgcaca cgctcgaggc ctccttcgtc ggcttcgaga
     1141 ccctccgccg ggtcgtggcc gtgcgcggca ccgagacgcg cgtcgacttc accctcacct
     1201 tctcgaacca ggcgatggag gccctcgagg tcttcgcctc ccgcgccgtc gagcggcgga
     1261 cgcccgtcgc gtactcgaac gtggacaagg tgcaggtgca gcgcgagctc ggctcgcgcg
     1321 acgtcccgat ggtgctgaac acgacgccgt cggtgtacgc gtcgcagagc ggcggcgggg
     1381 ccggtgatgc ccgcatcaac gtccgcggct tcaaccagcg caacgtcgcc atcatgatca
     1441 acggcgttcc cgtgaacgac atggagaacg gctgggtgta ctggtcgaac tgggacggcg
     1501 tcggcgacgc cacctcctct atccagctcc agcggggtct gtcggcggtg aacctcgcca
     1561 cgccctcgat cggcggcacg atgaacgtcc tcacggatcc ggcctcgatc gggcgtatgg
     1621 cgcgcatcaa gcaggaggtg ggcaacgacg gcttcctgaa gaccagcgtc ttcgccaaca
     1681 cgggcctgat gggcaacaag ttcgccgtat cgctggccgg cgtccggaag acgggcgacg
     1741 ggtactacga cgggacctgg accgacgcgt gggcctacta cggggccgtg agctaccagg
     1801 tcaacgcgaa gcaccgcctg gatctcttcg cgatcggcgc gccgcagcgg cacggccaga
     1861 acctgtacct gcagaacctc atggcctacg acgcggactt cgcggccgag gtctatgaga
     1921 aggacggcct tggccagagc acgatcgagg gcgcccaggc ctacttctgg gacgacaaga
     1981 actacaaggg ccgtcgctgg aacgagaacg tgagcgcggt ctcgagcgac tacaccctgg
     2041 agcagcacaa cggcttcggg atcgtggagc gcccggaaac gtgcacgatc gccggcgagg
     2101 aggtctcgtg catcaacgag cgcgagaact tcttccacaa gccgcagatc aacctgaact
     2161 ggtacgccca gttcaccgag aaactgctcc tctcgaccgt ggcgtactac tcgggcggca
     2221 agggcggcgg caccggcacg tccggctcga tggtgtggga ctacaccggg ccgagccgcg
     2281 tcgtcaacta cgacgccacg atcgcccgca acgagacgaa cgcgaccggc tcgcggggca
     2341 tcctgcgcaa cagccacaac gtgcaggaca cctggggcgc cattaccaag ctgaagatca
     2401 acgtgaacga ggcgctgacc gcggaggtcg gcctcgactg gcgcaccgcc gagatccgcc
     2461 actaccgcac ggtccgcgac ctgctcggcg gcgactacta cctgcgtgag gactcggact
     2521 tctgggacgg gccgcaggag ctcgccctgg gcgacgtgtt cgactacgac aacacgaaca
     2581 cggtcgactg gttcggcggc ttcgcgcagg ccgagtacgt gaaggacaac ctgtcgctgt
     2641 acggcatgac cggcgtttcg acgatcaagt acacgtttgt cgacgacttc gtcgatgcgg
     2701 gtgacggcac gccgcgcacg gtcgagacgg acaacatcta cggctaccag gtgaagggcg
     2761 gcggcctcta caacgtgggc gaggagctcg acgtgtacgc taacgccggc tgggtttcca
     2821 aggtaccgat cttcgacggc gtgatcgacg acgtcacggg cacgctcaac accgatccgc
     2881 agaacgagga gttcgtctcg gtcgaggccg gcgtcatgta ccggtcgcgc acgatgccgc
     2941 tgtccgtcgc ggccaacgtg taccggacca cctggaagaa ccgcaccatc gcgcgcgacg
     3001 tgacgctcgc cggcggaacc gagggcatcg tgaacctgct cggactcgac gcgctgcacc
     3061 agggcgtcga ggtcgaggcg gcgtaccagc cccttccctt cctgcggttc gacggcgtgg
     3121 cgtcgctcgg cgactggacg tacaccgagg acgtgagcgc ccgctacgtc ccggacggcc
     3181 gggagccgaa cctcgtcgag cagctccggc tctacacccg cgacctgaag gtcggcgacg
     3241 cgccgcagac ccagttcgtg ctgggcgtct cggtcttccc ggtcgacggg ctgtacctga
     3301 agctcctggg ccgctactac acggacttct acgccgagtt cgacccgctc tcccgcacgg
     3361 tggagggcga ccgcacccag tcgtggcaga tcccggagta cacggtcttc gacctgcacg
     3421 ccggctacaa cgtgccgatc acgtcgaacc gcttcgatgt tgcgatcttc gcgaacgtct
     3481 tcaacgtgtt cgacgagacg tacgtgctgg aggcgatcga caactcgtcg ttcaacgcct
     3541 acacggccaa cggcgtgaac catcgcgcgg acgacgccga ggtgttcctg ggcctgccca
     3601 cgaccttcaa cctcggtctc acggtgacct tccgctgatc cggcggaccg atcgtcacgc
     3661 tgccccggca gcgtgagcgc aaagcccccc gtccggtcga ccgggcgggg ggtttgtcgt
     3721 ttcggggcgc cgcggcggac gtcggcccgt cccctccgca ccccggccgc gtcgccgatc
     3781 cgcgggctca ggcgccggcc gccgggcgct ccccgcgccg cacgatcacg taggccacga
     3841 gcgcgaacgc gaagaacacg agcagcatcc aggcgacgcc ctgcatcgtg ccgatgagcg
     3901 tccggtacag ctccaggatc cagcgatccg agttgagcac gatcgggttg tcgggagcgg
     3961 gcgtgatgta cttctccagg tcccagacgc ggacgccacc cgagattccg accacgtaga
     4021 tggcgaacgt caggtaccgc ttccacgcgg cgccgaactc cgcgcccacg atccggtcca
     4081 gggtgcggtc gatgggtccg cggaagatcc gcgcgatgac gaaagagcag atcgccgcga
     4141 gcgcgagggt cacgacgagc agggagacga acatggcact tcctccgttg gtgcatacac
     4201 ggacgccccc ctgccgctga tgcgcaagcg gggacgcccc gacagggtga cgcgcctaac
     4261 gccccccggc cgcaagccag gcggtgcgga tgcggcgctg caggtcggtc gggccctcgg
     4321 acggctcgaa ggcgtagacc ggatccgagg cgtcgagcac caggcggacg gtccgctgct
     4381 cgccccagcg ctccaccgtg agttcgactt cgtcgccggg cgcacggccg tcgagggaga
     4441 atccgctctc cccgtcggcc ggactgccgt cgaccgccac gatgacgtcg tccacgtcga
     4501 gccctgcccg gaactctccg gagcccggcc gcacggacgt tacgcgcagg ccgtcgagct
     4561 cgacgcccac cctcaccggt cgccgctcgt ccggcctcct ggccagccgg agacccgcat
     4621 ggaccagggc ggcgtcccag tcgacggccg cggtcgagtc cacgtgcgct gcgaagaacg
     4681 cctggaacgt ggtgcccgtc acggcctcca gcgccctctg gaacccgtcc tcgggcactc
     4741 cctcgccgtt tctgtagtac gtgtcgtaga ggtgccgcat gacgtcgtcg aggctcttct
     4801 ccccgcccgt gcggccccgc acctcgaggt cgagcacgag ccccagggcc ttgccggccg
     4861 tgtagaacga gtagaaggtg aagggcggcg gatcgtcctg cttggcccag ctgtcgaagc
     4921 tggacatggc gacggacgtc accctccggc cgggatcgag gtcgaatgcg cgcaccgtgc
     4981 ccgcaagccc gccgagaaag gcctcctcgg aaatcaggcc gccgcgcagc agggccaggt
     5041 ccgcgtagta gtccgtgatc ccctcgaaga tccacatctg cgtcgtgtac tgctcccg
//
LOCUS       DBNHQZ010000141        17584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3425998, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000141 DBNHQZ010000000
VERSION     DBNHQZ010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 17584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17584
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3425998"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16YU_03685"
     CDS             complement(<1..184)
                     /locus_tag="AC16YU_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064355.1"
                     /translation="MTTRKRASGRTLRGMILSMLVWAALAGATSANQDPERIRTLIHV
                     LDYVAADYSAAVQDGVV"
     gene            complement(299..2332)
                     /locus_tag="AC16YU_03690"
     CDS             complement(299..2332)
                     /locus_tag="AC16YU_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HIR6064356.1"
                     /translation="MSVLTPLVAVTLPFLGDVVLLLLLCVAIAYLCYRLRIETIVGFL
                     LAGVVVGPSALRLVRDPELVGSMAEVGVILLLFTIGLEFSLEKLGRIRAYIVTGGGLQ
                     VGLTVAAVTGMLALAGVAFPSAIFTGFLVALSSTAIVLKLLADRRETDTPSGRLSLAV
                     LIFQDLAIIGMVLLVPMLSGRGGTWIDAVLALGKSAVIIGVTLVVARRIVPAILGAVA
                     RAQRPDLFLLTVVLICLGTAWTLSLFDVSLALGAFLAGLLVSESRYSSYALSEVLPLR
                     MLFNAVFFVSVGMLLDVGFLVGHLPLVLAATLGVLVLKAVLTSAAVLALRYPVRVAVV
                     VGIGLAQIGEFSFVLQSVGLNAGLTPLGIGETGTQYFIAVTVLLMTVTPFLVRAAPAV
                     GGWIQRFMPAGGAEPADAPPEAQREDHVIVVGYGAAGQRLAHVLGECGLGFVVVELNP
                     VLVDLAREEGVPVIQGDAGRPHMLQVAGIERAKLLVSVINDENATLQIVRMVQHLSPT
                     TQVIVRTRFLQSIEPLQRAGADIVVPEELETTVRLFSHMLGAYLIPPEEIERQTAALR
                     SADYRLFRGSIQEAHLMVLQGLDEEGLHTRAVGVRPGAPAAGRTLTELALRRDHNLTV
                     LAVRREGRTIGNPSGDFRVEPGDRLVLVGTAVQFAASADLWRTPEDATPKPGV"
     gene            complement(2329..4671)
                     /locus_tag="AC16YU_03695"
     CDS             complement(2329..4671)
                     /locus_tag="AC16YU_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6064357.1"
                     /translation="MDLSLPNLLLVLFVAWTAGRLASRMGYPSVLGELLAGIVLGPPL
                     LGLLHADAALEVLAELGILLMMVYIGMEIDPRELRRASKAGLLAAIGGFITPFVLCYF
                     AVLGFGGTVMMGVFVGVAAGVTSLATKSRILVDLHLLDTRIAHVLMAGALIADTVSLI
                     IFAGVLGAAEAGSVDATGIALIAAKAVAFFAVTILVGIKVFPRVGRLLEAAGITGRTA
                     SFTLVLILALAFAELAHLAGMHGILGAFIAGLFLREGVFGKARSQELTEVVRDASIGF
                     LAPIFFVSAGFAVSLGVFTTSLGLLVLVVGLATVGKIVGTALFYLPTGYGWREGLTVG
                     AGMNGRGAVEIIIAQIGLTMGLITQEIFSILVFMAIVTTATVPVLLKWGVDWLRGRGE
                     LVRSGDDRKGVLIVGGGAVSRAMGRILAGSQAVSVVDRNPFNCEAATAAGLTAVRGNA
                     LDEVVLSEAGAASVRHAIVTTPNAEVNALATQILRSTFLVPEISVLSSGDPQGHAALI
                     QHLRAVTLFGRSVNLLEWDYLVDQGQADIIPIEVIGEPREAGPFLDSASHPGALPVAV
                     RRGGEFQPFSADLVLEPGDAILLLEHTQTGEERRDAFDRLVAECPVLDLRDPLTSVEF
                     FDRVAGVMGARLQVGVDTLASMLCERERQGSTVFLPGLAIPHVVVPGEDVFDLVIARC
                     SPGITFPGDAGSVQSVFVLAGSADQRTAHLRALSAIAQTVQGPDFERSWHDAPDAEAL
                     RRLVLGANRRRFSSTVRGPSGEAVDSRTVPPTIRSDADRS"
     gene            4740..5237
                     /locus_tag="AC16YU_03700"
     CDS             4740..5237
                     /locus_tag="AC16YU_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR6064358.1"
                     /translation="MIRIPWDSSPACPAAAMPLSDLVSTLQLSIGPVILISGVGLLLL
                     SMTNRLGRVIDRSRILADGMAATVEADRPLVIAQLRILAARARIVRRAIALATFSVLL
                     AALLIITLFAGVLLDLAITGVLIGLFVVCMLALIASLLFFLSDINLTLRALWLEIPPE
                     AKEPV"
     gene            5328..5753
                     /locus_tag="AC16YU_03705"
     CDS             5328..5753
                     /locus_tag="AC16YU_03705"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6064359.1"
                     /translation="MRTFETTIDIQAPPERVWDVVADIPRWPEWTPSVLHAAALDEGP
                     TRIGSRFRLRQPGNLPAVWTVTEWAPGSGFEWVARFPGLRATAGHSIRTVDGGARLTL
                     RVTYSGPLAGLVAVLAGPMTRRFLGMESAGCRARSEGAV"
     gene            6011..7432
                     /locus_tag="AC16YU_03710"
     CDS             6011..7432
                     /locus_tag="AC16YU_03710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR6064360.1"
                     /translation="MSIGTTLTPSRRLESLDVLRGLTIAGMLLVNNPGTWDHIYWPLE
                     HAEWNGWTLTDLVFPFFLFMVGMAMMYSFPKRLERGDSKRELMLHVVRRAVALMLLGW
                     WGSTWSRLLWPGDAFHETSGFGTLAAVVLRLGFAAAVLAAVVLLAGTRRPRAWWIAFG
                     VGLVLAVGGLAVLGFDDSLARALAGIRIPGVLVRIGVCYLLASAIYFATPDPRAIAWW
                     IVGLLGAYALWMLFVPVPGFGVPDLDRGFPTAGTPAGELFSNWCFFIDYHVLGSHTWS
                     FRQIYDEAGRLVWSFDPEGVLSTVSAVGSVLLGVHAGMWMRRTDLDEPTKLNGLFVGA
                     SWLMGLGLVMSIWLPINKNLWSSSYTVFSAGMALLCLAVLYHAVDLKGWRTWAVPLRA
                     YGRNAIFAFVASGMMTTALGRITVSAFAGEGTTSAKEWTYDLLMAATGDAKFASFVFA
                     VAFVAVWALVAWWLDRRKVYFKV"
     gene            7579..8862
                     /locus_tag="AC16YU_03715"
     CDS             7579..8862
                     /locus_tag="AC16YU_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064361.1"
                     /translation="MPPTPRSAIRYERFLPCLALCLAALPGRAQPFPELLPQPVPGTP
                     REPFYSLSSRGDTVLAGGPGVVWFSVNRGRNWVAANALPDGATSVDAVVATDAGWFAS
                     AYGRGVYRSTNEGKDWTAVNAGLVGLGALDVWDFEVHGTDLYAGTQGAGVFRLDLGDP
                     DHWTSAGSEFALHEASTVESIALRGDVMVAAAGGNGLVFVRDLTSGPWVPAQLPYVIY
                     SVQASATGFVASGRFAAFWSEDDGATWQQTGPLAGSGPSATLAAWDDPAAATEAWLAQ
                     IVSGTESLLYRSADFEQPWEVLGLVPQTLAMLMYDGLLTMAQSDGLRVLDPAGGTGVD
                     PGAPAVDGLALDPPWPNPARDRVTLSCASGLPGSLRVEVLDALGRRVWSGDLRASGSP
                     GDHVAIDVAGWSPGLYLARISGGSRAASRAFVVRD"
     gene            9031..9492
                     /locus_tag="AC16YU_03720"
     CDS             9031..9492
                     /locus_tag="AC16YU_03720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6064362.1"
                     /translation="MPLKALSRFLDDHDIRYVTIRHSPAFTAQQIAASAHIPGKEIAK
                     TVMVRIDGKMAMAVLPGSLKIDLDRLREVAGAEVVEIATEQEFSNLFPECETGAMPPF
                     GNLYDMPVFVAESLAEDEEIAFNAGTHTELLQLAFDDFARLVHPTLGRFAR"
     gene            10009..11319
                     /locus_tag="AC16YU_03725"
     CDS             10009..11319
                     /locus_tag="AC16YU_03725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6064363.1"
                     /translation="MQFSNVGLSAELLRAVKDQGYETMTPIQEKAIPLALEGRDILAG
                     AQTGTGKTAGFALPLLQRLQDSDPSGRQRRIRVLILTPTRELAAQVESSVRDYGRYLP
                     FRTAVIFGGVGFHPQASRLRRGVDIVVATPGRLLDHMGQGTIDLRAVDTLVLDEADRM
                     LDMGFIPDIRRILKVLPRKRQNMLFSATFSREIRDLANALLDSPTEIQVAARNTAAEQ
                     ISQVAHPVDRTRKRELLSHMIGEGDWRQVLVFTRTKHGANRLSKQLTQDGHSATAIHG
                     NKSQTARTRALADFKDGRVRILVATDIAARGLDIVRLPHVVNFDMPLVPEDYVHRIGR
                     TGRAGLDGRAVSLVSVDENGLLQGIERLLKRDIERVVIPGFEPNGGFHAAPAERENGR
                     GNGRGNGGRNGRNGHGGRNGRSRETGPLSGERWGRGRTATDRRR"
     gene            complement(11340..12422)
                     /locus_tag="AC16YU_03730"
     CDS             complement(11340..12422)
                     /locus_tag="AC16YU_03730"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6064364.1"
                     /translation="MPFYDRPFLTRHPSGVRTLERLLALRRRLDDPANGVPQRTQEET
                     LLLATWNIRDFDKPTFGKRSEEAMHYIAEVVARFDLVAIQEVYRDLEALERLMKLLGG
                     NWKYIVTDTGEGSAANDERFAFVYDTRKVRFAGLAGEVVLPPVVIDGERRPPRQLART
                     PYICGFRSGWTDFVLCTVHILYGSGAPDHPERVEEIRQIARFMKDRSTDPAAWSQNVV
                     VLGDFNIFSRDDITMQALTDQGFLIPEELQEIPGTNVPKNKHYDQIAFRVRPGRLDTT
                     GRAGVFDVFNVIYRDEDEALYVDEMGDAYHTTSDGTPRQDPGQYYRTYWRTHQISDHL
                     PMWIELRINYTDAYLERLHTEATGGG"
     gene            complement(12548..12919)
                     /locus_tag="AC16YU_03735"
     CDS             complement(12548..12919)
                     /locus_tag="AC16YU_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064365.1"
                     /translation="MPTVRIPFTIPDVSDTDEAEGFLYLEDDYLVVEYTVKKFGIFRK
                     ATRMVKADRTVIVALELRKGLFGDRLVIETSSMQLLREVPGKHVTGVEVRTRRSHREA
                     VQRFIERVHAWVDRAGPVPRA"
     gene            complement(13060..14472)
                     /locus_tag="AC16YU_03740"
     CDS             complement(13060..14472)
                     /locus_tag="AC16YU_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6064366.1"
                     /translation="MRADGPSSGALVRGLTLVPAAALIVANVIGTGIFVKARVMTCNV
                     GTPGLVLSAYAVAGLFTLAGALTLAELGAMMPRSGGIYNFIGAAFGRVWAFLYGWMNT
                     FVNGAGSGAAIAIVFVIFLNDLVSGALSPGTAKAVAVGTLAAVTALNLASVRANGLVA
                     TILTVLKILMVFGIGAAAFLFGEGSMGHFAASGAAGTCEGVAEGARLGATGFGAAVVG
                     ALWSYHGWAVVTNVAEEVRDPGRTVPRALVGSSVLVIGLYVLVNAGYFYILSPEAVAS
                     TPEASSVAAEVVMRLIGASGAVVMAAGLLVSSFGTLHAHILTVSRVLFAMARDGLLPR
                     ALARLSRRGGVPATAIVVLGACAMVFALSGTFDLITDLVVFAVLIFNGLAVASVYVLR
                     RRLPDAPRPYRVRGYPVVPALFLVATLALTVNTLVATPGRALAGLGIIALGLPVYLWY
                     ARRLPETRAEDWLAPTDADR"
     gene            complement(14472..15893)
                     /locus_tag="AC16YU_03745"
     CDS             complement(14472..15893)
                     /locus_tag="AC16YU_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6064367.1"
                     /translation="MSAIPDRPRTLVRGLTLIPAAALVVTNVIGTGVFVKARVMTCNV
                     GTPGMVLLAYVMGGLLVLAGALALSELAAMMPRSGGMYNFLGAAFGRVWAFLFGWMET
                     FVDTAGSSAAIAIVFGIFVNDLLGGVLSVWQTQTVAVAIILVMIGVNLASVHTNGVLA
                     TIVTTIKVLVVVGVGVAAFVLGDGTLANFWVSGADGTCEGVAESARLGVTGFGAAVVG
                     ALWSYNGWTIATFVAEEVRRPERTLPRALVGSSVLLVVVYLIANAGYYFVMSPSEVAS
                     VAESSSVAGEVLVRIMGASGAALMTVGMMISCIGALHSTILSGSRLPFAMARDGLLPG
                     VMARVSRRSRIPAMGVIVVGVTAIVFAVSGTFDLITDLIVFAVLIFNGLAVAAVYVLR
                     RRMPAIRRPYRVPGYPWVPALFLLATLYLMVNTLVATPGRAIAGLGIIALGLPVYAWY
                     ARRLPSSRPEDFFEVDDDSGAGR"
     gene            complement(15901..16101)
                     /locus_tag="AC16YU_03750"
     CDS             complement(15901..16101)
                     /locus_tag="AC16YU_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064368.1"
                     /translation="MIGTTLSHYRIEAELGRGGMGVVHRAVERYERLLALWKDADPVM
                     QPRIDEIGRRRDAALDRLAEEA"
     gene            16439..16660
                     /locus_tag="AC16YU_03755"
     CDS             16439..16660
                     /locus_tag="AC16YU_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064369.1"
                     /translation="MKVFALILIVAGIIGIVIGSFSFTTDTTEVELGGLELQVSEERT
                     VNIPLWAGVGAIALGGGMLFAAAKGAKSA"
     gene            complement(16875..>17584)
                     /locus_tag="AC16YU_03760"
     CDS             complement(16875..>17584)
                     /locus_tag="AC16YU_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064370.1"
                     /translation="DTQSDWRLIITAAPFDIDDEHSVTFGLVMGEDLADLQANSDMMG
                     IVYHLEAAGYVIGEWVGDGMLGKGPGKALLSKLQVALSSIERANDRAAINHLGAFINS
                     VDGLVDEGVLDDAAGQYLIGHAMAIIELLGGDAGKGGIEPVTGETPGGVDLAQNYPNP
                     FNPTTLIRFELPETARVRLLVLDVLGREVARLVDGIRQAGAFEEVFNAATLPSGVYFY
                     RLETPQATITRRMLLVR"
BASE COUNT         2809 a         6243 c         5955 g         2577 t
ORIGIN      
        1 tcacgacacc gtcctgcacc gcggcggaat aatccgcggc cacgtagtcg agcacgtgga
       61 tcagggtgcg gatccgctcg gggtcctggt tcgccgacgt tgccccggcc agggcagccc
      121 agaccagcat cgacaggatc attccgcgga gcgtgcgccc gctggccctc ttgcgcgtcg
      181 tcatcggcgg atttcctgtc ggggtccggt cgtatttatt tagactgaat ctaaacaaac
      241 ggtccagtcc cggcaatccc cgtgccgcgg agtccgcatg cagaagggtg tgacccgctc
      301 agacgcccgg cttgggagtg gcatcctcgg gggtccgcca caggtcggcg ctcgccgcga
      361 actggacggc cgtaccgacg agcaccaggc ggtcgcccgg ttccacgcgg aaatcaccgg
      421 acggattgcc gatggtccgt ccttcccgcc ggaccgcgag caccgtcagg ttgtggtccc
      481 ggcgcagggc gagctccgtg agcgtccggc cggccgcggg ggctcccggt cgcaccccga
      541 cggcccgggt atgcaggccc tcctcgtcca ggccctgcag caccatgagg tgcgcctcct
      601 ggatgctccc ccggaacagc cggtagtccg cggaccgcag cgcggcggtc tgccgctcga
      661 tctcctccgg cgggatgagg taggccccga gcatgtggct gaacaggcgc accgtcgtct
      721 cgagctcttc cggaaccacg atgtccgccc ctgcccgctg cagcggctcg atcgactgca
      781 ggaaccgggt gcggacgatc acctgcgtcg tcgggctcag gtgctgcacc atgcgcacga
      841 tctgcagggt cgcgttctcg tcgttgatca cggacacgag cagcttggcg cgctcgatgc
      901 ccgcgacctg aagcatgtgg ggccgcccgg cgtccccctg gataacgggg acgccctcct
      961 cccgggccag gtcgaccagc acggggttca gctcgacgac gacgaagccc agcccgcatt
     1021 cgcccagcac gtgggcgagg cgctgtccgg ccgccccgta cccgaccacg atgacgtggt
     1081 cctcgcgctg ggcctcgggc ggggcgtccg ccggctcggc cccgccggcg ggcatgaatc
     1141 gctggatcca gccgcccacc gccggcgccg cgcggaccag gaacggggtg accgtcatga
     1201 gcaggaccgt cacggcgatg aagtactggg tccccgtctc cccgattccc aagggcgtga
     1261 ggccggcgtt caggccgacg ctctgcagca cgaacgagaa ctcgccgatc tgcgcgagcc
     1321 cgatcccgac gacgacggcc acgcggacgg ggtagcggag cgcgagcacc gctgccgagg
     1381 tcaggacggc cttcaggacc agtacgccca gggtggccgc caggaccagg ggcaggtgcc
     1441 cgaccaggaa tccgacgtcg agcagcatgc ctaccgagac gaagaacacc gcgttgaaca
     1501 gcatgcgaag cggcagcacc tcgctcagcg cgtagctgct gtagcggctc tcgctcacga
     1561 ggagtccggc caggaacgcg ccgagggcca ggctgacgtc gaacaggctc agggtccacg
     1621 ccgtgcccag gcagatgagg acgaccgtca gcaggaacag gtcggggcgc tgggcccgcg
     1681 ccaccgcgcc caggatcgcc ggcacgatcc tgcgggcgac gaccagcgtg acgccgatga
     1741 tcacggccga cttgccgagc gcgaggaccg cgtcgatcca cgtgccgccc cggccgctga
     1801 gcatgggcac gagcagcacc atcccgatga tggcgaggtc ctggaagatg agcacagcga
     1861 gcgaaaggcg gccggacgga gtgtccgtct cgcggcgatc ggccagcagc ttcagcacga
     1921 tggccgtcga cgacagggcg accaggaatc ccgtgaagat cgccgacgga aaggccacgc
     1981 cggcgagtgc gagcatgccc gtcaccgcgg ccaccgtcaa cccgacctgc aggccgccgc
     2041 ccgtcacgat gtatgcccgg atccggccga gcttttccag gctgaactcc aggccgatcg
     2101 tgaagagcag caggatgacc cccacctcgg ccatgctccc gaccagctcc gggtcccgca
     2161 ccaggcgcag ggccgacggt cccacgacga cgcccgcgag caggaacccc acgatggtct
     2221 cgatccgcag ccggtagcac aggtaggcga tggccacgca gaggagcagg agaagcacca
     2281 cgtcgcccag gaagggcagg gtgacagcca ccaggggagt cagcacgctc atgaccgatc
     2341 ggcgtcggat cgaatggtcg ggggcaccgt cctactgtcc accgcctcgc cggacgggcc
     2401 gcggaccgtg gaggaaaacc tccgccggtt tgcaccgaga acgagccgac gcagggcttc
     2461 cgcatccgga gcgtcatgcc acgaacgctc gaaatccggg ccctggacgg tctgtgcgat
     2521 ggcggacagg gccctcagat gtgcggtccg ctggtctgcc gagccggcga gcacgaagac
     2581 gctctggacg gatcccgcgt cgcccgggaa cgtgatcccc gggctgcagc gcgcgatgac
     2641 caggtcgaag acgtcctctc ccggcaccac cacgtgcgga atcgccagcc ccggaaggaa
     2701 gacggtgctg ccctggcgct cccgctcgca cagcatggac gccagcgtat cgaccccgac
     2761 ctgcagccgg gcgcccatca cgccggccac ccggtcgaag aactccacgc tggtgagagg
     2821 atcgcgcagg tccaggaccg ggcattcggc cacgagccgg tcgaacgcgt cgcgccgctc
     2881 ctccccggtc tgggtgtgct cgaggaggag aatcgcgtct ccgggctcga gcaccaggtc
     2941 ggccgagaac ggctggaact ccccgccgcg gcgcacggcc acgggcagtg cgcccggatg
     3001 ggaagccgag tccaggaacg gcccggcctc gcgaggctcc ccgatcacct cgatcgggat
     3061 gatgtccgcc tgcccctggt ccacgaggta atcccattcg aggaggttga ccgaacgtcc
     3121 gaacagggtc acggcgcgca ggtgctggat gagcgccgca tgtccctgcg gatccccgct
     3181 gctcagcacc gagatctcgg gcacgaggaa ggtggagcgc aggatctggg tcgcgagcgc
     3241 gttcacctcg gcgttcgggg tggtcacgat ggcatggcgc acactcgcgg cgccggcctc
     3301 gctgagcacc acctcgtcga gcgcgttccc gcgcacggcc gtcagcccgg ccgccgtggc
     3361 agcctcgcag ttgaacggat tccggtccac gaccgacacg gcctgggacc cggccaggat
     3421 ccggcccatg gccctcgaga cggcaccgcc accgacgatc aggacgccct tgcggtcgtc
     3481 gcccgagcgc acgagctcgc cccgtccccg gagccagtcg acgccccact tgaggagaac
     3541 cggaacggtg gccgtcgtga cgatcgccat gaagaccagg atcgagaaga tctcctgggt
     3601 gatgagaccc atcgtcagtc cgatctgggc aatgatgatc tccaccgccc cgcgcccgtt
     3661 catgccggcg ccgaccgtga gcccctcgcg ccagccgtag cccgtgggca ggtagaagag
     3721 cgccgtgccc acgatcttgc cgacggtcgc gaggcccacg accaagacca ggagcccgag
     3781 gctcgtcgtg aagacgccca gggagacggc aaacccggcc gaaacgaaga atatcggggc
     3841 gaggaatccg atcgaggcgt cgcggaccac ctcggtcagc tcctggctgc gcgccttgcc
     3901 gaagacgccc tcgcgcagga acagcccggc gatgaacgcg cccaggatgc cgtgcatccc
     3961 ggccaggtgg gcgagctccg cgaacgcgag ggccaggatc aggacgagcg tgaacgacgc
     4021 cgtgcgaccc gtaatgccgg cggcctcgag gagacggccc acccggggaa agaccttgat
     4081 cccgacgaga atggtcaccg cgaagaaggc cacggccttc gccgcgatga gggcgatccc
     4141 cgtggcatcc accgatccgg cctcggccgc gcccagtacg ccggcgaaga tgatcaggga
     4201 aaccgtgtcg gcgatcagcg cgccggccat cagcacgtgg gcgatcctgg tgtccaggag
     4261 gtgcaggtcc acgaggatgc gcgatttggt cgcgagcgag gttacgccgg cggcgacgcc
     4321 gacgaaaacc cccatcatca ccgtgccgcc gaaccccagc acggcgaaat agcacagcac
     4381 gaacggcgtg atgaagccac cgatcgcggc caggagcccg gccttcgacg cccgccggag
     4441 ctccctcgga tcgatctcca tgccgatgta caccatcatc agcaggatgc cgagctccgc
     4501 gagcacctcg agcgccgcgt ccgcgtgaag cagcccgagc agcggagggc ccaggacgat
     4561 gcccgcgagc agctccccca gcacggacgg gtaccccatc cgcgaggcca ggcgcccggc
     4621 ggtccaggcg acgaacagga cgagcagcag gttgggaagc gacagatcca taaacctcac
     4681 gttcgcgggt tccgccaggg aatcccgtgt tccttcgtca tccgggtgcc gtttcggcca
     4741 tgatacgcat accttgggac agttcgcccg cgtgtcccgc cgccgccatg ccgctctccg
     4801 acctcgtctc caccctgcag ctgtccatcg ggccggtgat cctcatctcc ggggtaggcc
     4861 tgctgctgct ctcgatgacg aaccggctgg gtcgggtcat cgaccggtcc aggattctcg
     4921 cggatggcat ggcagccacg gtcgaggccg accggcccct ggtcattgcc cagctccgga
     4981 tcctcgccgc ccgagcccgt atcgtacggc gcgccatcgc gctcgccacg ttcagcgtac
     5041 tgctcgccgc cctgctcatc atcacgctgt ttgcgggggt actgctcgat cttgcaataa
     5101 cgggtgtcct gatcgggctg ttcgtcgtgt gcatgctcgc cctgatcgcc tcgctgctct
     5161 tcttcctctc ggacatcaac ctcacgctca gggcactctg gctggagatt ccccccgagg
     5221 cgaaggagcc ggtgtgagcg cctgcgacgg gcaatccgca accctcggtg acggccaccg
     5281 tctactgcgg cggagcctga cgatgccccg ccgcccggac cgccgacatg aggaccttcg
     5341 agacgaccat cgacatccag gccccgccgg agcgggtctg ggacgtggtg gcggacattc
     5401 cgcggtggcc cgagtggacg ccgtcggtac tgcacgcggc cgcgctcgac gagggcccga
     5461 cccgcatcgg ctcccggttc cggctccggc agcccggcaa ccttccggcc gtgtggaccg
     5521 tcacggaatg ggctccgggg tccggattcg agtgggttgc ccgctttccg gggctccggg
     5581 cgaccgccgg acattccatc cggaccgtcg acggcggcgc gcggctcacg ctgcgcgtga
     5641 cgtactcggg accgctcgcc ggcctcgtcg cagtcctcgc cgggcccatg acgcggcgct
     5701 tcctgggcat ggagtccgcg ggctgccggg cgcggagcga aggcgcagtc tgacggcggc
     5761 gtcccgctcc ccagggagtt tcccgaagcg atcggttgtc caagcgggcg accgttccac
     5821 ctgcaggact ggatcgcgga ggtaggttcc tggtgtccgt gggcggcgga ggtcgtggac
     5881 gtcgaaaggc gttagggccc gttcacgcgt cccggggcgt cgcccgacgc ggcggaattg
     5941 cgtagcttcg tccggccgga caggtgttcc ggcgccgatc atccagtccg ggacggagcc
     6001 tcaggccgac atgtccatcg gaaccaccct taccccgtcc aggcgcctgg agtcgctcga
     6061 cgtccttcgc ggactgacga tcgccgggat gctcctcgtg aacaaccccg gtacctggga
     6121 ccacatctac tggcccctgg agcatgccga gtggaacggc tggacgctga cggacctggt
     6181 gttcccgttc ttcctgttca tggtcggcat ggccatgatg tactcgttcc cgaaacgcct
     6241 ggagcgggga gattcgaagc gcgagctgat gctccacgtg gtgcggcggg ccgtcgcgct
     6301 catgctcctc ggctggtggg gatcgacgtg gtcgaggctc ctctggccgg gcgacgcctt
     6361 ccacgagacg tcagggttcg gcaccctagc ggccgtcgtc ctgcggctgg gcttcgcggc
     6421 ggcggtgctc gcggccgtcg tcctgctcgc cggcacccgg cggccgcgcg cgtggtggat
     6481 cgcgttcggt gtcggcctgg tgctcgccgt cggaggcctc gcggttctcg gcttcgacga
     6541 ctcgctggcc cgggccctgg cgggcatccg gatcccgggc gtgctggtgc ggatcggcgt
     6601 ctgctacctc ctcgcgtcgg cgatctactt cgcgacgccg gaccctcggg cgatcgcgtg
     6661 gtggatcgtg gggctcctgg gcgcatacgc cctgtggatg ctgttcgtgc cggttccggg
     6721 gttcggcgtg cccgacctgg accgcggatt cccgaccgcc gggacccccg cgggcgagct
     6781 cttctcgaac tggtgcttct tcatcgatta ccacgtgctc ggaagccaca catggagctt
     6841 ccgccagatc tacgacgagg cggggcgcct ggtctggtcc ttcgatcccg agggcgtgct
     6901 gagcaccgtt tcggccgtcg ggtccgtgct tctcggggta cacgcgggca tgtggatgcg
     6961 ccgcacggac ctggacgagc cgaccaagct gaacggacta ttcgtcggag cgtcctggct
     7021 catgggcctc ggcctcgtga tgagcatctg gctgcccatc aacaagaacc tctggtccag
     7081 ttcgtacacg gtgttctcgg ccgggatggc gctcctgtgc ctggccgtgc tctaccacgc
     7141 ggtcgacctg aagggctggc ggacgtgggc ggtcccgctg cgcgcctacg ggcgcaacgc
     7201 aatcttcgcc ttcgtggcgt ccggcatgat gaccacggcg ctcgggcgga tcacggtcag
     7261 cgcgtttgcg ggcgagggga cgacgtcggc caaggagtgg acatacgacc ttctcatggc
     7321 ggcgaccggc gacgcgaaat tcgcgtcgtt cgtgttcgcg gtggcgttcg tcgccgtctg
     7381 ggccttggtc gcctggtggc tggaccggcg gaaggtctat ttcaaggtgt agccgggccg
     7441 cgcgggcgtc cccggggtcg accggatccc ggtcgcgcgc gcccggggcc ggcgaagatt
     7501 taatcgacgg actcctgccc ccgccgggtg ggaattcacg atcttcgtcg ccccgttttc
     7561 ccgacccgat cccgaggcat gccgcccacg ccccgatccg ccatccggta cgaacgcttt
     7621 ctcccctgcc tggcgctgtg cctggccgcg ctccctggcc gggcgcagcc ctttcccgag
     7681 cttctgccgc agcccgtgcc gggcacgccc cgggagccgt tctactcgct ttcctcgcgc
     7741 ggagacacgg tgctcgccgg ggggccgggc gtcgtctggt tctccgtgaa ccgcggcagg
     7801 aactgggtag cggcgaacgc gcttcccgac ggggcgacct ccgtcgacgc ggtcgtggcg
     7861 accgatgccg gatggttcgc atcggcctat ggccggggcg tgtaccggag cacgaacgag
     7921 ggcaaggact ggaccgccgt caacgccgga ctcgtcggcc tgggagcgct cgacgtgtgg
     7981 gacttcgagg tgcacggaac ggatctctac gcgggcacgc agggtgccgg ggtgttccgg
     8041 ctggacctgg gcgatcccga ccactggacc tcggccggta gcgagttcgc gctgcacgag
     8101 gcctccaccg tggaatcgat cgcgctccgc ggcgacgtga tggtcgcggc cgcgggcggg
     8161 aacggcctag tattcgtacg cgacctgacg tccggaccct gggtgccggc gcagcttccg
     8221 tacgtgatct actcggtgca ggccagcgcc accggcttcg tcgcgagcgg tcggttcgcg
     8281 gcgttctggt cggaggacga cggggcgacc tggcagcaga ccggccccct cgccgggagc
     8341 ggaccgtccg ccacgctcgc ggcctgggac gatccggcgg ccgcgaccga ggcctggctc
     8401 gcccagatcg tgagcggcac ggagagcctc ctctaccgct cggcggactt cgagcagccc
     8461 tgggaggttc tcggcctcgt tccccagacc ctcgcgatgc tcatgtacga cggcctgttg
     8521 accatggccc agtccgacgg cctgcgcgtg ctggatcccg ccggcggaac cggggtggat
     8581 ccgggcgcgc ccgccgtcga cggtctcgcg ctggatccgc cctggccgaa cccggcgcgc
     8641 gaccgagtca ccctctcctg cgcatcgggc ctcccgggct cccttcgcgt cgaggtgctg
     8701 gacgcgctgg ggcggcgtgt gtggtcgggc gacctgcggg cgtccggaag ccccggggac
     8761 cacgtggcga tcgacgtggc cggctggtcg cccggactgt acctggccag gatctccggc
     8821 ggctcgcgcg cggcctcgcg ggcgttcgtg gtgcgggact gaccgcgcga atgcggcgcg
     8881 aaatgcccct cggcggcttg acgccccgcg tcggacgggc tacaataccc tggaaccggc
     8941 ccgcttgcgg ggcgggatgg tggctgaaga gggccggtgc tcccccgttc atcccgcgcg
     9001 gcccacgctg cgccagacca ggaggacgtc atgccactca aagccctgag tcgcttcctg
     9061 gatgatcacg atatccgcta cgtgaccatc cggcactccc ccgccttcac ggcgcagcag
     9121 atcgccgcgt cggcacacat cccgggaaag gagatcgcga agaccgtcat ggtccgcatc
     9181 gacggaaaga tggccatggc cgtcctgccg ggatcgctca agatcgacct ggaccgcctg
     9241 cgcgaggtcg ccggtgcgga ggtcgtcgag atcgccaccg aacaggagtt ctcgaacctg
     9301 ttcccggaat gcgagaccgg cgccatgccc ccgttcggca acctgtacga catgccggta
     9361 ttcgtcgccg aatcactggc cgaggacgag gaaatcgcgt tcaacgccgg cacgcacacg
     9421 gagctgctgc agctcgcgtt cgacgacttc gcccgcctgg tgcatcccac gctcgggcga
     9481 ttcgcccggt gatgcgggat tccacggtgc gacgggtccc acccgttccg tcgacgtgag
     9541 cccgcgcgac cccgggggcc gcctggagtc ccggaatcgg tccactgggc cgccttggag
     9601 tcattttggc gctcggtgga actatccgac agcgcagccc ttacattggg ctgtgcgaat
     9661 tacgaatcgt gtcatttctt gagcggggtt cacggccatt cctgactgca agctcttcga
     9721 gcgcttctcc tgcagcccgg ctccggccca cccgacccca gtcgtggaat accgcgagat
     9781 agcctccgga cctgtccgga cggggtatcc gcagacgcaa ttctctctgt cggacgactg
     9841 cctgatgtag tccgtccgat cccttccggc gccttcggtc tgcgccgacc cctccatcgc
     9901 cgcacctttg cggatcgtgg agaaccaccc ggaccccgtc ccgaccgacc cgcaacgatc
     9961 gccgagcgcg acaggcgggt gggacgatcc agggacataa cctgtcgaat gcaattctcg
    10021 aatgtcggcc tctcggccga actgctgcgt gccgtcaagg accaggggta cgaaaccatg
    10081 accccgatcc aggaaaaagc gataccgctc gccctcgagg gccgcgatat cctggccggc
    10141 gcgcagaccg gaaccggcaa gaccgccggc ttcgccctcc ccctcctcca gcggctccag
    10201 gactccgacc cctcgggccg gcagcgccgg atccgcgtcc tgatcctgac tcccacgcgg
    10261 gagctcgcgg cccaggtcga atcgagcgtc cgtgactacg gccgctacct cccgttccgc
    10321 acggccgtca tcttcggcgg cgtcggcttc catccccagg cctcgcggct gcgccgcgga
    10381 gtcgacatcg tcgtggccac cccgggccgc ctgctcgacc acatgggcca gggcaccatc
    10441 gacctgcggg ccgtggacac cctcgtgctc gacgaggccg accggatgct ggacatgggt
    10501 ttcatccccg acattcggcg gatcctcaag gtcctgcccc gcaagcggca gaacatgctc
    10561 ttctcggcga ccttttcccg ggagatccgg gacctggcca atgcgctcct cgactcgccg
    10621 accgagatcc aggtggcggc ccgcaacacg gcggccgagc agatctccca ggtcgcccac
    10681 ccggtggacc gcacccgcaa gcgggagctg ctctcgcaca tgatcggcga gggtgactgg
    10741 cgccaggtgc tcgtgttcac ccgcacgaag cacggcgcga accgcctgtc gaagcagctc
    10801 acccaggacg gccactcggc gaccgccatc cacggcaaca agtcgcagac ggcccgcacc
    10861 cgggcgctcg cggacttcaa ggacggccgc gtccgcatcc tcgtggccac cgacatcgcg
    10921 gcccggggac tggacatcgt gcggcttccg cacgtcgtca acttcgacat gccgctcgtg
    10981 cccgaggact acgtgcaccg catcggccgc accggccggg cgggactcga cggccgcgcc
    11041 gtgtcgctgg tctcggtgga cgagaatgga ctgctccagg gcatcgagcg cctgctcaag
    11101 cgggacatcg agcgcgtcgt gatcccgggc ttcgagccga acggcggctt ccatgccgct
    11161 ccggccgagc gcgagaacgg gcgcggaaac ggacgcggca acggcgggcg caacggacgc
    11221 aatggccacg gcggccgcaa cggacgctcc cgcgaaaccg gaccgctctc gggcgagcgc
    11281 tggggccgcg gccggaccgc gaccgaccgg cggcgctgac ggagccggcg cggcggggct
    11341 cacccgccgc ccgtcgcctc ggtgtgcagg cgctcgaggt acgcgtccgt gtagttgatc
    11401 cgcagctcga tccacatcgg caggtggtcc gagatctggt gcgtgcgcca gtacgtccgg
    11461 tagtactggc ccgggtcctg gcgcggcgtg ccgtcgctcg tcgtgtggta ggcatcgccc
    11521 atctcgtcga cgtagagcgc ctcgtcctcg tcccggtaga tcacgttgaa cacgtcgaac
    11581 acgccggcgc ggcccgtcgt gtccagccgg cccgggcgga cgcggaacgc gatctggtcg
    11641 tagtgcttgt tcttcggaac gttcgtgccc gggatctcct ggagctcctc gggaatcagg
    11701 aatccctggt ccgtgagcgc ctgcatggtg atgtcgtcgc ggctgaagat gttgaaatcg
    11761 cccaggacga ccacgttctg gctccaggcc gcggggtcgg tcgagcggtc cttcatgaag
    11821 cgggcgatct ggcggatctc ctcgacccgc tccggatgat ccggggcgcc gctcccgtac
    11881 aggatgtgga cggtgcacag cacgaagtcc gtccatccgc tgcggaatcc gcagatgtac
    11941 ggcgtgcggg cgagctggcg agggggacgc ctctcgccgt cgatcacgac cggtggcagc
    12001 acgacctcgc cggccagccc ggcgaagcgc acctttcgcg tgtcgtagac gaaggcaaag
    12061 cgctcgtcgt tggccgccga gccctcgccg gtgtcggtca cgatgtactt ccagtttccg
    12121 ccgaggagct tcatcaggcg ctcgagcgcc tcgaggtcgc ggtacacttc ctggatggcc
    12181 accaggtcga aacgggcgac cacctcggcg atgtagtgca tcgcctcctc ggatcgcttc
    12241 ccgaacgtcg gcttgtcgaa gtcgcggatg ttccaggtgg cgagcagaag ggtctcttcc
    12301 tgggttcgct gcggcactcc gttcgcgggg tcgtcgagcc gacggcgcaa cgccagcagg
    12361 cgctcgagcg tgcggacacc ggacggatgg cgggtcagaa acggtcgatc gtagaagggc
    12421 atggggccgg gtggtgggtt cggggtccca cactaaaggc aatgccggca cgaagcgcaa
    12481 gggtcggcgg tcatcgactc gcgcggcgcc ctgttactcc tcggatggcc cgttcgcgcc
    12541 aggcccgtca cgcgcgcggc acgggccccg cgcggtccac ccacgcatgc acgcgctcga
    12601 tgaaccgctg cacggcctcg cggtgcgatc gcctggtccg gacctcgacg cccgtcacgt
    12661 gcttcccggg cacctcccgc agcagctgca tgctggaggt ctcgatcacg agacggtccc
    12721 cgaacaaccc cttgcgaagc tccagcgcca cgatcaccgt gcggtcggcc ttcaccatgc
    12781 gcgtcgcctt ccggaagatg ccgaacttct tgaccgtgta ctcgacgacg agatagtcat
    12841 cctcgaggta caggaagccc tcggcctcgt ccgtgtccga gacgtcggga atcgtgaacg
    12901 ggatgcgcac ggtgggcatg cgggtcttct ggcatacgga ctacaggaac gcaagatagc
    12961 ccgaaccggg ctccggacca gcgcaggagc gatccgcgtg ccggcatgct tcccgggtcg
    13021 cgcgcgaatg gatggtggcc gggcgcacgc cgggcccggc taccggtccg cgtccgtcgg
    13081 cgcgagccag tcctccgccc tcgtctccgg caggcgccgc gcataccaca ggtagaccgg
    13141 cagcccgagg gcgatgatgc ccaggccggc cagcgcgcgc cccggagtcg ccaccagcgt
    13201 attcaccgtg agggcgagcg tggccacgag gaaaagggcc ggcacgacgg gatagccgcg
    13261 gacccggtac ggccgcgggg catccgggag acggcggcgc aggacgtaca ccgaggcgac
    13321 ggcgaggccg ttgaagatca ggacggcgaa gaccaccagg tccgtgatga ggtcgaacgt
    13381 gcccgagagc gcgaatacca tggcgcacgc gccgagcacg acgatggccg tggccgggac
    13441 gccgccccgc cgcgacagcc gggccagcgc gcggggaagc agcccgtcgc gggccatggc
    13501 gaagagaacc ctggagaccg tcaggatgtg cgcgtggagc gtcccgaagg acgagaccag
    13561 gaggcctgcc gccatgacca ccgcgcccga cgccccgatc agccgcatga cgacctcggc
    13621 cgccacggaa gacgcctcgg gcgtgctggc gacggcctcc gggctcagga tgtagaaata
    13681 cccggcgttg accagcacgt agagcccgat gaccagcacc gagctgccga ccagggcgcg
    13741 gggcacggtc cgcccggggt cacgcacctc ctcggccacg ttggtcacca ccgcccagcc
    13801 gtggtagctc cacagcgcgc cgacgaccgc ggccccgaag cccgtcgcgc ccagccgggc
    13861 gccctcggcc accccctcgc acgtgcccgc ggctccggag gcggcgaagt gccccatcga
    13921 gccctcgccg aagaggaacg cggccgcccc gatcccgaag accatcagga tcttcaggac
    13981 cgtgaggatc gtggccacga ggccgtttgc ccgcaccgag gccaggttca gggcggtgac
    14041 cgcggccagc gtccccacgg ccacggcctt cgccgtcccg ggcgagagcg cccccgagac
    14101 gaggtcgttc aggaagatga cgaacacgat cgcgatggcg gcgcccgatc ccgccccgtt
    14161 cacgaacgtg ttcatccagc cgtacaggaa ggcccagacg cgcccgaagg ccgcgccgat
    14221 gaagttgtag atgccgcccg accggggcat catcgcgccg agctcggcga gcgtgagcgc
    14281 gcccgcaagc gtgaagagcc cggcgaccgc gtaggcgctg agcacaaggc cgggcgtgcc
    14341 cacgttgcag gtcatcacgc gggccttcac gaatatgccc gtcccgatga cgttcgccac
    14401 gatcagggcc gccgccggaa cgagcgtgag cccccggacc agggcaccgg aggatggccc
    14461 gtccgcccgc atcaccttcc cgcccccgaa tcgtcatcca cctcgaagaa gtcctccggc
    14521 ctcgacgacg gcagccgccg ggcgtaccag gcgtagacgg gcagcccgag cgcgatgatg
    14581 ccgagccccg caatcgcccg tccgggggtc gcgacgagcg tgttgaccat caggtacagg
    14641 gtcgcgagca ggaagagcgc cggcacccac gggtagccgg ggacacggta cggccgacgg
    14701 atggccggca tcctgcggcg cagcacatac accgccgcga cggcgagccc gttgaagatg
    14761 agcacggcga acacgatcag atccgtgatc aggtcgaacg tgccggagac ggcaaacacg
    14821 atcgccgtca cgccgaccac gatcaccccc atcgccggga tgcgcgatcg gcgcgacacc
    14881 cgggccatca cgcccggcag gagcccgtca cgcgccatcg cgaacggcag gcgcgacccg
    14941 gacaggatcg tcgagtgcag ggccccgatg caggagatca tcatgccgac ggtcatgagc
    15001 gcggcgcccg acgcgcccat gatgcggacg agcacctccc ccgccacgga cgacgattcg
    15061 gcgacgcttg ccacctcgga cggcgacatg acgaagtagt agccggcgtt cgcgatcagg
    15121 tagacaacga ccagcagcac gctgctgccg acgagcgccc tcggcagcgt ccgctcgggg
    15181 cggcgcactt cctcggcgac gaaggtcgcg atcgtccagc cgttgtagct ccagagagcc
    15241 ccgacgacgg ccgcgccgaa cccggtcacg ccgagccgtg cgctctcggc cacgccctcg
    15301 caggtgccgt ccgccccgct cacccagaag ttcgccagcg tcccatcgcc gagcacgaac
    15361 gccgcgacgc cgacgccgac gaccacgagc accttgatcg tcgtcacgat ggtggcgagc
    15421 accccgttcg tgtgcaccga ggcgaggttc accccgatca tcaccaggat gatcgcgacc
    15481 gcgacggtct gcgtctgcca gaccgacagc acgccgccca gcaggtcgtt cacgaaaatg
    15541 ccgaacacga tcgctatcgc ggccgacgat cccgccgtgt cgacgaacgt ctccatccac
    15601 ccgaacagga acgcccagac gcgcccgaag gccgcaccga gaaagttgta cataccaccc
    15661 gagcgcggca tcatcgcggc gagctccgag agcgcgagcg cgcccgcgag caccagcagc
    15721 ccgcccatga cgtaggcgag gagcaccatg ccgggcgtgc ccacgttgca cgtcatgacc
    15781 cgtgccttga cgaagacgcc cgtcccgatg acgttcgtca cgacgagcgc cgccgcgggg
    15841 atgagcgtaa ggccgcggac cagggtgcgg ggacgatcgg gtatcgcgga catgtccggt
    15901 tcaggcttcc tcggccaacc ggtcgagcgc cgcatcccgg cgccggccga tctcgtcgat
    15961 ccggggctgc atcaccgggt cggcatcctt ccagagggcg agcaggcgct cgtaccgctc
    16021 gacggcccgg tgcacgactc ccatgccgcc gcggccgagc tccgcctcga tgcggtagtg
    16081 cgagagggtc gttccgatca tgggttccgg gagtgggcgg aagacggagg ccgcagggac
    16141 ggccgttgag ggccgccagg gcggccaggg gggtgccgcc gggggatcat accgattcgg
    16201 tgtgcggccc gcaataccgg agggccggcg cgcggcctgc cggccgccgg gaggaacgcg
    16261 tcccttcacg cctcgcgcga tgtcgcccgc aacgggcgaa gaacaagtcc ggcacgggga
    16321 ccgttcacca tgccgacatc cgctcctggt tcgggccgtt ccgcccggcc tcgtgaggaa
    16381 gcctgctcgc cgcgcccgac cggacgccca cctccctccc accactccag gtgtcgtcat
    16441 gaaggtattc gcactcatcc tgatcgtcgc ggggatcatc ggaatcgtaa tcggcagctt
    16501 cagcttcacg acggacacca ccgaggtgga actcgggggg ctggagctcc aggtctccga
    16561 ggaacggacg gtcaacatcc cgctctgggc gggcgttggc gccatcgccc tgggcggagg
    16621 catgctcttc gcggcggcta agggggcgaa atcggcctag gcaattacgg ccggctgcgg
    16681 gattgagccg ccgtactttt ccccgcggtg cggttttccg ctgaggcaac tcgtcgtccg
    16741 gcaagcacaa tgccgggcgc tgcatgtgcc gatcgaacga ctccggccat tcggcgtcgt
    16801 acggcggaac ggctatctcc ccggtcgcga accgctgaat tcaccgctcc ggcgcttcca
    16861 cggcttgcgg cgcactaccg gacgagcaac atcctccttg tgattgtcgc ctggggcgtc
    16921 tcgagacggt agaagtacac gccactcggc agagtcgcgg cgttgaacac ttcctcgaat
    16981 gcaccggcct ggcggatgcc atccaccagc cgagccactt cccgtcccag gacgtccagt
    17041 accagaagtc gaacccgggc cgtctccggc aattcgaatc ggatcagcgt cgtcggattg
    17101 aacggattcg gatagttctg cgcgaggtcc acgccaccgg gagtctcccc ggttaccggt
    17161 tcgatgcctc ccttgccggc gtctccgccg agcagttcga tgattgccat ggcatggccg
    17221 atgaggtact ggccggcggc atcgtcgagc acaccctcgt cgacgaggcc gtccacgctg
    17281 ttgatgaaag cgccgagatg gttgatcgcg gccctgtcat ttgccctctc gatgctggag
    17341 agggccacct gcagcttcga gagaagtgcc ttgcccggcc ccttgcccag catgccgtcc
    17401 cccacccact ccccgatcac gtatccggcg gcctcgagat ggtacacaat ccccatcatg
    17461 tcggagttgg cctggaggtc ggccagatcc tcccccatga ccaggccgaa ggtcacggag
    17521 tgctcatcat cgatatcgaa aggcgcggcc gtgatgatca gccgccaatc ggactgggta
    17581 tcgg
//
LOCUS       DBNHQZ010000142         5577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_989147, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000142 DBNHQZ010000000
VERSION     DBNHQZ010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_989147"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="AC16YU_03765"
     CDS             <1..1347
                     /locus_tag="AC16YU_03765"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6064371.1"
                     /translation="PGFIDIHSHADGSLVRLPLAENYVRQGVTSVFGGQDGDSPLDLG
                     TFLGGFDLEPAAVNLGMFVGHGSVRERVVGLDDRPATGAELEGMARLVDRAMRDGAFG
                     LSSGLEYTPGRFAPTDELVALAAATAPWGGLYISHVRDEGGRLEESVREVIRIAEEGR
                     VAGQLTHHKIIGKDRWGGTERSLALVDEARSRGVDVTLDVYPYTASSTGMTILFPAWA
                     QDGGFEALQARLRDPADRERIRGDVIRHLVAERGGDPSTVVASLCTWDSTRNGKSLAD
                     MARESGREATVETAADIAIELVEGGNCQGVFHSMSEDDVRRVMDHPFAMIASDGGVPE
                     PGRGMPHPRSYGTFARVLARYVRELGTLTLPDAVRRMTYAPAARLGLRDRGVIRPGAV
                     ADVTVFDPATIRDEATFTDPHRYATGVRHVFVAGRWTLRDGEPTGVRAGRTLRRGR"
     gene            complement(1348..2259)
                     /locus_tag="AC16YU_03770"
     CDS             complement(1348..2259)
                     /locus_tag="AC16YU_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6064372.1"
                     /translation="MRSWPVVVLCVLVSMPARAQRAAVDPELQRAVEQAVAGFQGRVG
                     VYVRHLPTGRIAALNSNALFPTASLVKVPILIATFDAIEQGRLEWRQELVYRDSLLYE
                     GEDILGSFRDGAVIPLSQVAMLMITTSDNTAALWLQQLCGTGTAVNGWLEKQGFGGTR
                     VNSRTPGRRSAWQLYGWGQTTPREMADLFVMIREGRAVSPAASDEMYRMLTRIYWNDE
                     ALSQLPPWVQAASKQGSVGDARSEVVLVNAPSGDYVFAVMTDDQKDRSWDHDNAGFEL
                     IRRISAMLWNRFEPDHPYEPPDDPRYR"
     gene            2526..5330
                     /locus_tag="AC16YU_03775"
     CDS             2526..5330
                     /locus_tag="AC16YU_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6064373.1"
                     /translation="MLHRFESPLHGVRWIGVLLALVALEALPAYGQTGSVSGRVIDAT
                     DGLPLAGANVLVFGGNGVMVTGAATGEDGTYRIVAVPAGSYRLQARFLGFQEYESQIR
                     VTGGETLTVDVSLSATGFELNTVVITGSRREEKVLEAPASMSVLTSEDVTRSVGSSSI
                     EALRNTTGVDMAQTGIDRREVVLRGFNNAFSGAAYVLTDYRHAAVASLAVNIYSIMPN
                     MNVDVDRVEVVRGPGSALYGAGVDSGVIHFISKDPFAHPGTTVSLSAGERNFVGLQFR
                     HAGVLGARQNLGYKITGMFGQADDWAMNPHDPIDAVQLQGDVVLRNTDYNKSNVNGTL
                     EYRFSERGSVIASGGYSNLDATVLSGIGTLQAENFGYTYGQLRFQYDRFFAQAYFNKN
                     RAGGSFVYDQDFDGDDVPDGVTDNGIMYNVQAQYDFDLGRNQNFIAGVDAELTRPDTE
                     GTILGRNEDNDQVSEYGAYIQSLTRITPKLDLTLALRGDHNNVVDKFQVSPRAALVVK
                     PMDGHSFRVTYNRAFSSPSTNSNFLDIIAATSPLTVRARGSAYGFTWERDQAGNLLAT
                     SLLPNRLGQAHAIGAPLDAIYELVYAGIAAIPTATLTALLQQQGLMIDQQTTAALVQL
                     LSPSATTVSGLSPGVLALPNLTTGQTEIGVPEPVDVRPLDQTITQTIEVGYKGLFRNR
                     LLVTIDAYWTNKKDFVGPLLLESPLVVTPSLANDLTGALATGIQGNAQLAGAIAQMGL
                     TPAQVAAIVVGFAEEDLPAAVGIVQAVENDPGVGVAPEMMLTYRNFGNLSYTGVDVAF
                     EFAASDRVSIFGNASWVSDDFFDETELDEEGTGLELALNASALKGRLGVSYVTGAGLS
                     VSVAGRYTDGFPIASGPYVGDLPEYTVLDLNVGYDLGATVPGLRLDLGISNVLDERHR
                     EFIGAPEMGRLGMLRATYSF"
BASE COUNT          873 a         1956 c         1891 g          857 t
ORIGIN      
        1 ccgggcttca tcgacatcca ctcccatgcg gacggaagcc tggtccggct gccgctggca
       61 gagaactacg tccgccaggg cgtgacgagc gtcttcggcg gacaggacgg cgattcgccg
      121 ctggacctcg ggacctttct gggcggattc gacctcgagc cggccgccgt gaacctgggc
      181 atgttcgtcg gtcacggctc cgtgcgcgag cgcgtcgtgg ggctcgacga ccgtccggcg
      241 accggggccg agctcgaagg gatggcccgc ctcgtggacc gggccatgcg cgacggggcc
      301 ttcggcctgt cctcggggct ggaatacacg ccgggccgct ttgccccgac cgacgaactg
      361 gtggccctgg ccgcggcgac cgccccctgg ggcgggctct acatcagcca tgtgcgcgac
      421 gagggcggac gcctggagga gagcgtccgc gaggtcatcc gcatcgccga agagggacgg
      481 gtcgcagggc agctcaccca ccacaagatc atcggcaagg accgctgggg gggcaccgag
      541 cgctcgctcg cactcgtgga cgaagcgcgc tcgcgcgggg tggacgtgac gctcgacgtg
      601 tacccctaca cggcctcgag caccgggatg accatcctct ttccggcgtg ggcccaggac
      661 ggaggcttcg aggcgctcca ggcacgcctc cgggatccgg cggaccggga gcgcatccgc
      721 ggggacgtca tccgccacct ggtcgccgag cggggcggag acccgtcgac cgtcgtggcc
      781 tccctgtgca cctgggattc gacccggaac ggcaagagcc tcgccgacat ggcccgggag
      841 tcgggtcggg aggcgaccgt cgagacggct gccgacatcg ccatcgagct cgtcgagggg
      901 ggcaactgcc agggcgtgtt ccactcgatg tcggaggacg acgtgcgccg ggtcatggac
      961 caccctttcg cgatgatcgc gtccgacggg ggcgttccgg agccgggtcg ggggatgccg
     1021 catcccagga gctacgggac gtttgcccgc gtgctcgcac gctacgtgcg cgagctcggc
     1081 acgctgaccc tcccggacgc cgtccggcgc atgacgtacg cgccggccgc caggctcggc
     1141 ctccgcgacc gcggggttat ccggcccggc gcggtcgccg acgtcacggt gttcgacccg
     1201 gcgacgatcc gtgacgaggc caccttcacg gacccccacc gctacgccac cggggtgcgg
     1261 cacgtgttcg tcgcgggtcg gtggacgctg cgcgacggag agccgaccgg cgtgcgggcc
     1321 gggcggacgc tgcgccgcgg ccgctgatca gcggtaccgg ggatcgtccg ggggctcgta
     1381 cgggtggtcg ggctcgaacc ggttccagag catggccgag atccggcgga tcagctcgaa
     1441 gccggcgttg tcgtggtccc agctccggtc cttctggtcg tcggtcatga cggcgaacac
     1501 atagtctccc gacggggcgt tcacgagcac gacctccgat cgcgcgtccc ctacggatcc
     1561 ctgcttggag gccgcctgca cccacggcgg cagctgcgag agggcctcgt cgttccagta
     1621 gatgcgggtg agcatccggt acatctcgtc gctcgccgcg gggctcacgg cccggccctc
     1681 ccggatcatc acgaagaggt ccgccatctc gcggggcgtc gtctgtcccc agccgtagag
     1741 ctgccaggcc gaccggcggc cgggggtccg ggagttcacc ctagtgccgc cgaagccctg
     1801 cttctccagc cagccgttca cggcggtgcc cgttccgcag agctgctgaa gccagagcgc
     1861 ggcggtgttg tcgctcgtgg tgatcatcag catggccacc tgcgatagcg ggatcactgc
     1921 gccgtcccgg aagctcccca ggatgtcctc gccctcgtac aggagggagt cccggtagac
     1981 gagctcctgc ctccactcga gccgcccctg ctcgatcgcg tcgaaggtgg ctatcaggat
     2041 cgggaccttg accaggctgg ccgtcgggaa cagggcgttc gagttcaggg cggcgatccg
     2101 ccccgtgggg aggtgccgca cgtatacgcc cacgcggccc tggaaccccg cgacggcctg
     2161 ctccaccgcg cgctggagtt cgggatccac tgccgcgcgc tgggcgcgcg cgggcatcga
     2221 gacgagcacg cacaggacga ccaccggcca gctccgcatg acggtacctc tctcttggcg
     2281 cccgaaggtc gtggaccgac gggctgccgc acaacggatc ggatcgcgag atttcaggga
     2341 atccccgtga acggtcgccc gggaggggcc atcggtcggt gcccgggcgc acgcggcggg
     2401 tgctccggcc ccgggaagcc atattgcgaa atatggacca ttagggtatc gttcccgggt
     2461 tggagccgca gctacccggc gccccggcat tcgcacattg accctcgcca aaccgcggag
     2521 aatccatgtt gcatcgcttc gaatctcccc ttcacggtgt acggtggatc ggcgtgcttc
     2581 tcgcgctcgt cgcgctggaa gcgctcccgg cctacggtca gaccggctcg gtctcgggcc
     2641 gcgtgatcga cgccaccgac ggactgccgc tcgccggcgc caacgtgctc gtgttcggcg
     2701 gcaacggcgt gatggtgacc ggggccgcca cgggtgagga cggcacgtac cggatcgtgg
     2761 ccgtcccggc cggttcctac aggctgcagg cccgcttcct gggcttccag gaatacgagt
     2821 cgcagatccg ggtgacgggc ggggaaacgc tgaccgtcga cgtgtcgctg tcggcgaccg
     2881 gcttcgagct caacacggtc gtcatcaccg gttcccggcg cgaagagaag gtgctggagg
     2941 cgccggcctc gatgtccgtc ctcacgagcg aggacgtgac gcgctcggtg ggatcttcct
     3001 cgatcgaagc gctccggaac acgaccgggg tcgacatggc ccagacgggc atcgaccgcc
     3061 gggaggtggt ccttcgcggc ttcaacaacg cgttctccgg ggcggcctac gtgctcaccg
     3121 actaccggca tgcggccgtc gcctcgctcg cggtgaacat ctactcgatc atgcccaaca
     3181 tgaacgttga cgtggaccgc gtcgaggtcg ttcgcggtcc gggctcggcg ctgtacggcg
     3241 cgggcgtgga ctcgggcgtg atccacttca tctcgaagga tccgttcgcc caccccggca
     3301 ccaccgtgtc gctctcggcg ggagaaagga acttcgtcgg cctgcagttc cgccatgccg
     3361 gcgtcctcgg agcccggcag aatctcggat acaagatcac ggggatgttc gggcaggcgg
     3421 acgactgggc gatgaacccc cacgacccga tcgatgccgt ccagctgcag ggtgacgtgg
     3481 ttctccggaa caccgactac aacaagagca acgtgaacgg cacgctcgag taccgcttct
     3541 cggaacgcgg atcggtgatc gcgagcggcg ggtactcgaa cctcgacgcc accgtcctct
     3601 cgggcatcgg caccctgcag gccgagaact tcggctacac gtacggccag ctccgtttcc
     3661 agtacgaccg cttcttcgcg caggcgtact tcaacaagaa ccgggcgggc gggtctttcg
     3721 tgtacgacca ggacttcgac ggcgacgacg tcccggacgg cgtgaccgac aacggcatca
     3781 tgtacaacgt ccaggcccag tacgacttcg acctcgggag gaatcagaac ttcatcgccg
     3841 gcgtcgacgc ggaactgaca cggcccgaca ccgagggcac catcctcggg cgcaacgagg
     3901 acaacgacca ggtctccgag tacggggcgt acatccagtc cctcactcgc atcacgccca
     3961 agctggacct gacgctggcg ctgcgcgggg accacaacaa cgtggtggac aagttccagg
     4021 tcagcccccg cgcggccctc gtcgtgaagc ccatggacgg gcactcgttc cgggtcacgt
     4081 acaaccgggc cttctcgtcc ccgagcacga actcgaactt cctggacatc atcgcggcga
     4141 cgtcaccgct caccgtccgg gcgcgcggct cggcctacgg cttcacctgg gagcgcgacc
     4201 aggcgggcaa cctgctggcg accagcctcc tgccgaaccg cctggggcag gcccacgcga
     4261 tcggggcccc gctcgacgcc atctacgagc tcgtgtacgc gggcatcgcc gccatcccca
     4321 cggccacact caccgccctg ctccagcagc agggcctcat gatcgaccag cagacgaccg
     4381 ccgccctggt ccagctcctg tccccgtccg cgaccacggt ttcggggctc tcgccgggcg
     4441 tactggcgct cccgaacctc accaccggtc agaccgagat cggcgtgccc gagcccgtgg
     4501 acgtcaggcc cctcgaccag accatcaccc agacgatcga ggtcggatac aagggcctgt
     4561 tccgcaatcg cctgctcgtg accatcgacg cgtactggac gaacaagaag gacttcgtcg
     4621 ggccgctgct gctcgagtct ccgctcgtgg tcacgccgag cctggcaaat gacctcaccg
     4681 gcgcgctcgc cacgggcatc cagggcaacg cgcagctggc aggagccatc gcccagatgg
     4741 gcctgacgcc cgcccaggtg gcagccatcg tcgtgggctt cgccgaggag gatctcccgg
     4801 ccgccgtggg catcgttcag gccgtcgaga acgatcccgg cgtgggcgtg gcgcccgaga
     4861 tgatgctcac ctaccggaac ttcggcaacc tgtcgtacac gggcgtggac gtggccttcg
     4921 agttcgccgc cagcgaccgg gtgagcatct tcggcaacgc ctcgtgggtc agcgacgact
     4981 tcttcgacga gaccgagctc gacgaggagg gaaccggcct cgagctcgcc ctgaacgcct
     5041 cggccctcaa gggccgcctc ggcgtctcgt acgtgacggg cgccggcctc tcggtctcgg
     5101 tggccggccg gtacacggac ggtttcccga tcgcgtccgg cccctacgtc ggcgacctgc
     5161 cggagtacac ggtcctggac ctgaacgtag ggtacgacct cggggcgacc gtcccgggcc
     5221 tcaggctgga cctgggcata tcgaacgtcc tcgacgagcg ccaccgcgag ttcatcgggg
     5281 ccccggagat gggacgactc gggatgctcc gggcgaccta ctcgttctga tcgatcgggg
     5341 tgccctctac aagaaaacgg ggccgccgca tgtgtgcggc ggccccgttc gtcttcatca
     5401 tgcccgcgaa ggctactggt tgaagaccat cccgagccga actccgaact ggctgaagga
     5461 ttccccgtcc tccgtcgccg tgtagtagag cggcccgccg tacagcgtac ccgggccgac
     5521 ggcacccgag gcgtagaggg ccacgttgat caggaacggg ttgctggtat tgctgtc
//
LOCUS       DBNHQZ010000143         2051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_991771, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000143 DBNHQZ010000000
VERSION     DBNHQZ010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_991771"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2051
                     /locus_tag="AC16YU_03780"
     CDS             <1..>2051
                     /locus_tag="AC16YU_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR6064374.1"
                     /translation="PADDLDYRVISELATDAIFVHDVRTGAILDANRQACELHGYTVD
                     EIRAGGVEAISDGNPPFDAEHAREHVRRAAAGEPQQFEWLCRKKSGARFWVDVRLHRV
                     SMRGEPRILAIVANHDERKRAEAALRASEESYRSLFDTLSELVYIQDLDGRFVNVNQA
                     VVQTYGYACGELIGKTPEILAAPGKVNVEDTMRRFRLAVEGEPQRFDWWGRRRDGSIF
                     PKEVVLTRSTYFGRDVIIAVARDISERVNAETALRRSEKLFRRMIENASDLISIMDAD
                     GTIRYQSPSVRRILGYEPEEMVGTNAFSFLPPEDVQTTAGRWKRVIDEPGTPVAAEFR
                     FRHRDGTYRHMESVGATLSPERPDDGVVVNSRDVSARKRAERALIRQNALLEAAWESS
                     IDGILVVSEDGRIESHNQRFAELWRIPDDVIASRSDEAAIEAVLSQVADPDAFLKRVG
                     YLYEHPDIRSRDTIALKDGRVLDRYTSPVKSGTGEYHGRIWFFRDVTPEKRHERELER
                     ARQEADHYAASLERELEFGRRVQQGLLPAALPQPAGWDLAACFRPALQVAGDFYDAFE
                     LPDGHLGLMIADVSGKGVGAALFMALFQGHLRAAVERGSPAEGDPDQESAETSGSRGE
                     GSDPDVLARAVADTNEYITRVHRHARMFASVFFGILDPETGTIRYVNAGHEPPVVIRA
                     D"
BASE COUNT          339 a          710 c          705 g          297 t
ORIGIN      
        1 cccggccgac gacctcgatt accgcgtcat cagcgaactc gcgaccgacg ccattttcgt
       61 gcacgacgtc cggacgggag cgatcctgga cgcgaaccgc caggcgtgcg agcttcacgg
      121 ctacacggtc gacgagatcc gggcgggcgg cgtcgaggcg atcagcgacg gcaatccccc
      181 gttcgacgcc gagcacgcgc gggagcacgt ccggcgggcc gcggccggcg aaccgcagca
      241 gttcgagtgg ctctgcagga agaagtcggg cgcgcggttt tgggtggacg tccggctgca
      301 ccgcgtgagc atgcgcggcg agccccgcat cctcgccatc gtggccaacc acgacgagcg
      361 caagcgggcc gaggccgccc ttcgcgccag cgaggagagc taccggtcgc tgttcgacac
      421 cctgtccgaa ctggtgtaca tccaggacct cgacggccgg ttcgtcaacg tgaaccaggc
      481 cgtggtgcag acctacggct acgcctgcgg cgagctcatc ggcaagacgc ccgagatcct
      541 ggcggccccg ggcaaggtga acgtcgagga caccatgcgc cgcttccgcc tggctgtcga
      601 gggcgagccg cagcgattcg actggtgggg ccggcgccgg gacggaagca tctttcccaa
      661 ggaggtcgtg ctcacgcggt ccacgtactt cgggcgggac gtgatcatcg ccgtcgcccg
      721 cgacatctcg gagcgcgtga atgccgagac tgccctgcgt cgcagcgaga agctgttccg
      781 gcgcatgatc gagaacgcct ccgacctcat ctcgatcatg gacgccgacg ggaccatccg
      841 gtaccagagt ccgtccgtcc ggcggatcct cggctacgag ccggaagaaa tggtcggaac
      901 gaacgccttc tccttcctac cgcccgagga cgtccagacc accgccgggc ggtggaagcg
      961 cgtgatcgac gagccgggca ctccggtcgc ggccgagttc cgcttccggc accgggacgg
     1021 cacgtaccgc cacatggaat cggtcggcgc gacgctttcg cccgaacgcc ccgatgacgg
     1081 cgtggtcgtg aactcgcgcg acgtcagcgc acggaagcgg gccgagcgcg ccctgattcg
     1141 gcagaatgcg ctgctcgagg ccgcgtggga gtcctcgatc gacggaatcc ttgtcgtttc
     1201 cgaggacggc cgcatcgaat cccataacca gcgcttcgcg gagctctggc gaatcccgga
     1261 cgacgtcatc gcatcgcgct cggacgaggc agccatcgag gccgtgctct cccaggtggc
     1321 cgatcccgat gccttcctga agcgggtcgg ctacctgtac gagcatcccg acataaggtc
     1381 gcgcgacacg atcgcgctga aggacgggcg ggtgctggac cgctatacgt ctcccgtcaa
     1441 gagcggcacc ggcgaatacc acggccgcat ctggttcttt cgcgacgtta cgcccgagaa
     1501 gcggcatgag cgtgagctgg agcgcgctcg ccaggaagcc gaccactacg cggccagcct
     1561 ggagcgcgag cttgaattcg gccgacgggt gcagcagggc ctgcttccgg ccgccctgcc
     1621 gcagccggcg ggctgggacc tggcggcatg cttccgcccc gcgctccagg tagccggcga
     1681 cttctacgat gccttcgagc tccccgacgg acacctcggc ctcatgatcg ccgacgtgtc
     1741 gggcaagggc gtgggggcag cgctcttcat ggcgctgttc cagggccacc tgcgcgccgc
     1801 ggtcgagcgg ggcagcccgg cggagggtga cccggaccag gaatcggcgg agacgtccgg
     1861 ttcacgcggc gaggggagcg acccggacgt tctcgcgcgc gcggtcgccg acacgaacga
     1921 gtacatcact cgcgtccacc ggcacgcccg catgttcgcg tccgtgttct tcgggatcct
     1981 cgatccggag accgggacga tccggtacgt caacgccggc cacgagccac cggtcgtgat
     2041 ccgggcagac g
//
LOCUS       DBNHQZ010000144         4038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_992114, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000144 DBNHQZ010000000
VERSION     DBNHQZ010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_992114"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16YU_03785"
     CDS             complement(<1..367)
                     /locus_tag="AC16YU_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HIR6064375.1"
                     /translation="MRLAVFVSGGGSNFQAILDAAAAGRLSARVVLCVSSRPDAGALE
                     RARRHGVPTAVLSAPREDDREVAREMLDLLSAHDVDFVALAGFMRRVPPEVVRAFRNR
                     MLNIHPALLPAFGGAGMYGR"
     gene            complement(441..1418)
                     /locus_tag="AC16YU_03790"
     CDS             complement(441..1418)
                     /locus_tag="AC16YU_03790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR6064376.1"
                     /translation="MPTEPPALPPALLPPASIGIISPASAPRDPSRAEAGMAALRERG
                     FTLVHEPPASGPRGYLAAPDADRTAELNAMLRRDDVDAILCTRGGFGVLRLLDTVDYD
                     AARRRPKLLVGYSDITALQLALYRHAGWRSLSGAMLAPDWPDLDGDSEAQFWNMARGA
                     TPSPFVGPGGEPLEGVRDGSAEGVLLGGNLSMVMRLLSTPFLPDLDGAILFFEDVGEA
                     PYRLDSYFAQLRLAGVLDRLGGVVVGALTDSQVEPGRPSLGIDEVLGDHLGRLPIPVA
                     RGLVYGHFPRKSTLPIGVGARLSVDGPDARLDILEPVTRRPDGSLFTAS"
     gene            1520..2185
                     /locus_tag="AC16YU_03795"
     CDS             1520..2185
                     /locus_tag="AC16YU_03795"
                     /inference="COORDINATES: protein motif:HMM:NF020463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HIR6064377.1"
                     /translation="MRVAVIAPEYFPGLATAALVAAVDLVVLADSTRYARQSGQNRAR
                     LRTPHGVQWITIPVGHGQHGRRISEMEIRENEPWRRAHLKALRFNYGATPYFEHYFMR
                     IEALLSAPAGSLADVTAASTRLVAELMGARPRWLRSSELPGSPGDLASILGALDSGRL
                     VLPGDAVVPGGAAPAGTERFRFRAPEYRQRFPGFRPGCSVLDMLFVHGPESRHLLLSG
                     VEA"
     gene            complement(2202..2555)
                     /locus_tag="AC16YU_03800"
     CDS             complement(2202..2555)
                     /locus_tag="AC16YU_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6064378.1"
                     /translation="MRTKSVSLNIVESAVPSPGAERIDVLAAGGAFRLERIVSLGQVT
                     PPGAWYDQGRPEWVMVVSGRARLRFDGEDRDLELAPGDTVLIEAGRRHRVTWTDPEVP
                     TVWLGLHFDPEPQDG"
     gene            2608..>4038
                     /locus_tag="AC16YU_03805"
     CDS             2608..>4038
                     /locus_tag="AC16YU_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR6064379.1"
                     /translation="MSRKVIATILLVLLAKSPVAAQKANFELAERFTTQRMDKMVGTL
                     DIRPRWIEDEPRFWYEFEASGGKAWYLVDAGRREKSLLFDQEALAARLTEHFRKPFNA
                     KDLPLKEFEYDTDRGVFRFHVDSIRFTYRFETRELAAGDSVQKEPDMAWATWSPDSTW
                     IAFARRHDLYLMRGNDPDSVEIRLTRDGERWYSYQAEDGDTTTTKRLRSRARWFEDEK
                     KLYVVRQDRRKVRDLWVINSLKARPELETYKYAMPGEDDIPIDEIWAFAVDDTVGVRL
                     DTDRWKSQSIGGVYFNRGGLFTSERSDHVWFLRRDRAWQNVDVCRGDTETGEVTVLWS
                     ERSEPYHNTRFMDLAVIGEGEEYLWWSERTGWGQLYRYGADGTLRNRITDGYFVVGDI
                     ERIDTLGQVVYFTGYGREEGVNPYYGMPYRVNFDGSGMELLAPEPVDHEVSISEDARY
                     IVDMMSSVDSTPRSVLRDARGRVIREL"
BASE COUNT          632 a         1370 c         1482 g          554 t
ORIGIN      
        1 ggcggccgta catcccggca ccgccgaagg ccgggagaag cgccgggtgg atgttcagca
       61 tgcggttccg gaacgcgcgc accacctccg gggggacccg ccgcatgaag cccgcgagcg
      121 ccacgaaatc gacgtcgtgg gcggagagca gatcaagcat ctcgcgcgcc acctcgcggt
      181 cgtcctcgcg gggagccgac agcacggcgg tcggcacgcc gtgtcggcgg gcccgctcca
      241 gggcgccggc gtccggccgg ctgctcacgc agagcaccac ccgcgcagag agccgtccgg
      301 cggcggcggc gtccaggatg gcctggaagt tggatcctcc gccggaaacg aagacggcga
      361 ggcgcatggg taactgaagc cggggctctg gaggcgggcg atgttactcc cccgcgccgc
      421 ggcgccccgt ccggtgggtg tcaagaggcc gtgaaaagcg atccgtccgg gcgccgggtc
      481 accggttcga ggatgtcgag gcgcgcgtcg ggtccgtcga ccgacaggcg cgccccgacg
      541 ccgatcggaa gcgtggactt ccgggggaag tgcccgtaca cgaggccgcg ggcaaccggg
      601 atcgggaggc gccccaggtg gtcgcccagg acctcgtcga tcccgaggga gggccggccg
      661 ggctcgacct gggagtcggt cagtgccccg accacgacgc cgcccagccg atcgagcacg
      721 ccggccagcc ggagctgggc gaagtacgag tcgaggcggt acggcgcctc gcccacgtcc
      781 tcgaagaaga ggatggcgcc gtcgagatcg ggcaggaagg gcgtggagag caggcgcatg
      841 accatcgaca ggttgccgcc cagcagcacg ccctcggccg atccgtcgcg gactccctcg
      901 aggggctcgc cgcccgggcc gacgaacggg gagggggttg caccgcgggc catgttccag
      961 aactgcgcct ccgagtctcc gtccaggtca ggccagtcgg gtgccagcat ggcgccggac
     1021 agcgatcgcc accccgcgtg gcggtagagc gcgagctgca gcgccgtgat gtcggagtat
     1081 cccacgagca gcttcggacg gcgccgggcc gcgtcgtagt cgaccgtgtc gagcagccgc
     1141 agcacgccga aaccgccgcg cgtgcacagg atggcatcga cgtcgtcccg gcgcagcatc
     1201 gcgttcagct ccgccgtgcg gtccgcgtcc ggcgccgcga ggtatcccct gggcccggag
     1261 gcgggcggct cgtggacgag cgtgaagccg cgttcccgca gggccgccat gccggcctcg
     1321 gcccgggaag ggtcccgggg cgcgctggcc ggggagatta tgccgatcga agccgggggg
     1381 aggagggccg ggggcagggc gggcggttcg gtcggcacgg ttcgcgcggg tggaaggcat
     1441 cgtcggggag cggggccaag atagcgaccg gcgccgagcc ccggcgggtc gcccgcggcg
     1501 gctgcgtacc ttcacgggca tgcgtgtcgc ggtcatcgcc cccgaatact tccccggact
     1561 cgccaccgcg gccctcgtgg cggccgtgga tctggtcgtg ctcgcggact cgacccggta
     1621 cgcgcgccag tccggccaga accgggcgcg cctccggacg ccgcacggcg tgcagtggat
     1681 cacgattccc gtcggacacg ggcagcacgg gcgcaggatc tcggagatgg agatccggga
     1741 gaacgagccg tggcgtcggg cgcacctcaa ggccctgcgg ttcaactacg gggccacacc
     1801 ttacttcgag cattacttca tgcggatcga ggcgctcctt tcagcgccgg ccggatcgct
     1861 tgcggacgtc accgcggcct ccacccggct cgtggccgag ctgatgggcg cccggccgcg
     1921 gtggctccgg tcgtcggagc tccccgggtc gccgggggac ctggcatcca tcctcggcgc
     1981 gctggattcc gggcggctgg tcctgccggg tgatgccgtc gtgccgggcg gcgcggcgcc
     2041 ggcgggcacg gagcgcttcc gcttccgtgc cccggagtac aggcagcgct ttccgggatt
     2101 ccggcccggg tgctccgtgc tggacatgct cttcgtgcac ggtccggaga gtcggcacct
     2161 gctcctgtcc ggcgtcgagg cctgattctc cgcgacgggc atcacccgtc ctgcggctcc
     2221 ggatcgaaat ggagcccgag ccacacggtg gggacctcgg gatcggtcca ggtcacgcgg
     2281 tgccgccggc cggcctcgat gaggacggtg tcgcccggcg cgagctccag gtcccgatcc
     2341 tcgccgtcga aacggagccg ggcccttccc gatacgacca tcacccactc cggccggccc
     2401 tggtcgtacc acgcgcccgg cggcgtcacc tggcccaggg agacgatccg ctccaggcgg
     2461 aacgcgccgc ccgcggccag cacatcgatc cgctccgcgc cgggcgacgg gacggccgat
     2521 tcgacgatat tcaacgatac gctctttgtc cgcatggatg ctggagccta tgctcgggtg
     2581 ccgcgcccac cagaatgccc cggagacatg tcccgcaagg tcatcgccac gatcctcctc
     2641 gtcctgctcg caaagtcgcc cgtcgccgcg cagaaggcaa atttcgaact cgccgagcgc
     2701 ttcaccacgc agcgaatgga caagatggtc gggacgctgg acatccggcc ccggtggatc
     2761 gaggacgagc ccaggttctg gtacgagttc gaggcgtcag gcggaaaggc gtggtacctg
     2821 gtcgatgccg gcaggcgcga gaagagcctg ctcttcgacc aggaggcgct cgcggccagg
     2881 ctcaccgagc atttccggaa gccgttcaac gcgaaggatc tcccgctcaa ggagttcgag
     2941 tacgacacgg accgcggcgt cttccgcttc cacgtggact cgatacggtt cacgtaccgc
     3001 ttcgagacgc gcgagctggc ggccggcgac tccgttcaga aggagcccga catggcgtgg
     3061 gcgacctggt ccccggactc cacctggatc gcgttcgcgc ggaggcacga cctgtacctg
     3121 atgcggggga acgaccccga ttccgtcgaa atccggctca cgcgcgacgg ggagcggtgg
     3181 tacagctacc aggccgagga cggggacacc accacgacga agcggctccg ctcgcgcgcc
     3241 cgctggttcg aggacgagaa gaagctgtac gtggtgcgcc aggaccgccg caaggtgcgc
     3301 gatctctggg tcatcaacag cctgaaggca cgcccggagc tcgaaaccta caagtacgcg
     3361 atgcccgggg aggacgacat cccgatcgac gagatctggg ccttcgccgt ggacgacacg
     3421 gtgggcgtca ggctggacac cgaccgatgg aagagccagt cgatcggagg cgtctatttc
     3481 aaccggggcg gactcttcac gtcggagcgg tccgaccacg tctggttcct gcggcgggac
     3541 cgcgcgtggc agaacgtcga cgtctgccgg ggcgacacgg agacgggcga ggtcacggtg
     3601 ctctggtcgg agcgcagcga gccctaccac aacacgcggt tcatggacct cgccgtgatc
     3661 ggggagggcg aggagtacct ctggtggtcg gagcggacgg ggtggggaca gctctaccgg
     3721 tacggagcgg acgggacgct caggaaccgc atcacggacg gatacttcgt cgtcggcgac
     3781 atcgagcgga tcgacacgct ggggcaggtc gtctacttca cgggatacgg ccgcgaggag
     3841 ggcgtgaacc cgtattacgg catgccctac cgggtgaact tcgacggctc cggcatggag
     3901 ctcctggcgc cggagccggt cgaccacgag gtctcgatct cggaggacgc ccgttacatc
     3961 gtcgacatga tgtcctcggt ggattcgacg ccgaggtccg tgctccgcga cgcccggggc
     4021 cgggtgatcc gggagctc
//
LOCUS       DBNHQZ010000145         3486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_582925, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000145 DBNHQZ010000000
VERSION     DBNHQZ010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_582925"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="AC16YU_03810"
     CDS             <1..1455
                     /locus_tag="AC16YU_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064380.1"
                     /translation="QGPPAYAPHLPVAALDFVALGGYDSAFWFVDADTLFAIELPDDS
                     VSIRRPVEDVLEVFELPYGPRWFSSSIDATTGTVWMWDGTVGPVARFGLRADAGRQSF
                     DVYLSRIQGGHGGGLHPRTGRPVAFGGYGYYRVHDLLVTFDDRARTWVEMATSSTDRP
                     EARMTAVMVTGWHPTKLVVVGGTGRASRDTPLRFDRVMADAWTYDVADSAWAPVPIPE
                     PVRCALASGAQVGDYLVEPGSRSVLLFSAYGCPPDPSHNSGEILRWYPESGGFETVAR
                     LGQALPVGYSVFGMVLSRDSSRIHLLVSKLVEGDHLARADRRVLSFTRTAASLVSAAT
                     GGASAGPWAVVGIGFLVMAVAMLVLWLLLRRKRVDGLVDVRMGGEGAPVFVRCGRRAR
                     SRPLSPNAHRLLAALLQWGVAPGDILPAERLGELFDDLYADPDSARVAKNRAVDELNQ
                     LLTQVCGGPALERVRRTDDARRFDWKVAANLRAA"
     gene            1845..>3486
                     /locus_tag="AC16YU_03815"
     CDS             1845..>3486
                     /locus_tag="AC16YU_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064381.1"
                     /translation="MRTLNRIFPLLAYALVAAAFWPVTASGQIVLDLTTEGAVADHNG
                     ARFIQEDASEGAGTGILDPFVRTKNDVGGVTASMNTGNSTVQFQTESGPWTEALLLST
                     VTDRDLGEFGIFKEFALDINQTAGNSIYGMTEVQLWADDQADLNCYNPAAGGSWGTCD
                     TGDAATRVYWLDQNTGGSFPGSTGDATVLMDYGLESGSGGQTDIVMLISIDLFPDAEN
                     CYYGSLTCSTYLYLYSRYGDDLVNGSPADDGFEEWSTLVEPFVLIDKTLAGSIDETHD
                     WNLTKDVDSLECEQFVGGTCTFNYTVEVTEIENLSLTIGGQVIYSVPAMLPDGDQAAP
                     DANVTVDSDVYSDIDGTVVWADPTCAIGSMFTLAAGESKLCVYSVNYADAGDADSGLQ
                     SNTLTASVEFNEGVFIGYAEAVIATNSNGPAAVDVTDTIEGLLGSGLTDGATFNYPQI
                     QECSADPGDYTDGAYSFDVTNIAEIVQTDDEDTETVTVNCFAPVVSKTADAEYTQHYS
                     WTIDKSVDPAEHHLFAGGSADSDYDVLVDLTYSEDGFAI"
BASE COUNT          557 a         1140 c         1198 g          591 t
ORIGIN      
        1 cagggtccgc cggcgtacgc gccgcacctg ccggtcgcgg ccttggattt cgtcgcgctg
       61 ggcggatatg actcggcgtt ctggttcgtg gatgccgaca cgctcttcgc catcgaactg
      121 ccggacgatt ccgtgagcat caggcggccc gtggaggacg tcctcgaggt tttcgaactg
      181 ccctacggtc cccggtggtt ttcttcctcg atcgacgcga cgacgggcac cgtctggatg
      241 tgggacggca cggtcgggcc ggtcgcacgg ttcggccttc gcgccgacgc aggcaggcag
      301 agcttcgacg tctacctgag caggatccag ggcggacacg ggggaggact gcatccgcgc
      361 accgggcgcc ccgtcgcctt cggcggatac gggtactacc gggtgcacga cctgctcgtg
      421 accttcgacg accgggcgcg gacgtgggtg gaaatggcca ccagcagtac ggaccggccg
      481 gaggcgcgga tgaccgcggt gatggtcacg gggtggcatc ccacgaagct cgtcgtcgtg
      541 ggcggcacgg gcagggcgag ccgggatacg ccgttgcgat tcgaccgcgt gatggcggac
      601 gcctggacgt acgacgtggc cgacagcgcc tgggcccccg tcccgatccc ggagcccgtc
      661 cggtgcgcgc tggcgagcgg ggcgcaggta ggcgattacc tcgtcgagcc cggatcgcgg
      721 agcgtgctgc tgttctcggc gtacggctgc ccgccggacc cgtcgcacaa ttcaggggag
      781 atcctgaggt ggtaccccga atcgggcggc ttcgagacgg tcgcccgtct ggggcaggcc
      841 ctgccggtgg ggtactcggt cttcgggatg gtgctttccc gggattcgag ccgcatacac
      901 ctcctggtgt cgaagctggt ggagggcgat cacctggccc gtgcggatcg ccgggtcctg
      961 tcgttcaccc ggacggcggc gagcctcgtg tcggcggcga cgggtggtgc gtccgccgga
     1021 ccgtgggcgg tggtcgggat cggcttcctg gtgatggccg tggccatgct ggtgctctgg
     1081 ctgctcctgc gccgcaagag ggttgacggc ctcgtggatg tgaggatggg cggggaggga
     1141 gcgccggtgt tcgtccgctg cgggcgccgc gcccgctcgc gccccctgag cccgaacgct
     1201 catcggcttc ttgccgccct gctgcagtgg ggggtcgcgc cgggcgacat cctgcccgcc
     1261 gagcgcctcg gcgagctctt cgacgacctg tacgcggatc ccgactcggc cagggtcgcc
     1321 aagaaccgcg cggtggacga gctcaatcaa ctgttgacgc aggtctgcgg cggtccggct
     1381 ctcgagcgtg tccgccggac ggacgacgcc cggcgcttcg actggaaggt ggctgcgaac
     1441 ctccgcgcgg cgtagcggac cgcagccgca gccggcaggg ctccgacgcg cgccgcaggc
     1501 tgtttcgatt ccggattccg aacgcggatc gcgaggcatg cggcttcggt gacggccgta
     1561 gggccttccg caggggcagg taggcatcct gctgaacaga tgatcggcat tcgtcctcta
     1621 tcccctttgc taccaaggga ttatgctgtt gtcatctact gtctggccgg aagccgcaac
     1681 ctgcggcacg gccgagcggc gggacccctc caccctggca cccccctctg ccatggctgc
     1741 ccaccgagtc gtcttgcacg atgcgcagcc ggtattgatc ggcgcctgcg atcggccccg
     1801 tcgtgtttcc gtgcaccgta ccacctgaac ggggaggtaa ccccttgcga acgcttaatc
     1861 gaatctttcc gctactggcc tatgcgctgg tggcggccgc cttctggccc gtcacggcgt
     1921 ccggccaaat cgtcctcgat ctgacgacgg aaggcgccgt cgccgatcac aacggcgccc
     1981 gcttcatcca ggaagacgcg tccgagggag ccggcaccgg cattctggac cccttcgtcc
     2041 ggacgaagaa cgacgtgggc ggcgtgaccg cgtcgatgaa caccggaaac tccacggtcc
     2101 agttccagac ggagagcggc ccgtggaccg aggcactgct cctcagcacc gtcaccgacc
     2161 gcgacctcgg tgagttcggc atattcaagg agttcgccct cgacatcaac cagaccgccg
     2221 gcaattcgat ctacggcatg accgaggtgc agctctgggc ggacgaccaa gcggacctca
     2281 actgctacaa tcccgcagcc ggcggctcgt ggggcacgtg tgatacgggc gacgcggcca
     2341 cccgggtgta ctggctcgac cagaacaccg gcggcagctt cccgggttcg accggcgatg
     2401 cgacggtcct catggactac ggcctggaat cgggcagcgg tggtcagacc gacatcgtga
     2461 tgctcatcag catcgacctg ttcccggatg cagagaactg ctattacggc agcttgacct
     2521 gcagcacgta cctgtacctg tactcgcgct acggcgatga cctcgtgaac ggatccccgg
     2581 ccgacgacgg cttcgaggag tggtcgacgc tggtggagcc gttcgtactc atcgacaaga
     2641 cgctcgccgg cagcatcgac gagacgcacg actggaatct cacgaaggac gtggattccc
     2701 tcgagtgcga acagttcgtc ggcggcacct gcacgttcaa ttacaccgtc gaggtgaccg
     2761 agatcgagaa tctctcgctt acgatcggcg gccaggtgat ctactccgtg ccggccatgt
     2821 tgccggacgg cgaccaggcg gcgcccgacg cgaacgtgac ggtcgacagc gatgtctact
     2881 cggacatcga tggcaccgtg gtgtgggccg atccgacctg cgcgatcggg tcgatgttta
     2941 cgttggcggc gggcgagtcg aagctctgcg tctacagcgt gaattacgcc gacgcgggag
     3001 acgcggattc cggtctccag tccaacacgt tgaccgcctc ggtggagttc aacgaaggcg
     3061 tgttcatcgg gtacgcggag gccgtcatcg cgacgaactc gaacggcccg gcggccgtgg
     3121 acgtcaccga cacgatcgaa ggcctgctcg ggagcggcct caccgacggc gcgacgttca
     3181 actacccgca gatccaggag tgctcggccg atcctggcga ctacacggac ggagcctact
     3241 cgttcgacgt gacgaacatc gccgagatcg tccagacgga cgacgaggac accgagaccg
     3301 tcaccgtgaa ctgcttcgcg ccggtcgtct ccaagacggc ggacgccgag tacacgcagc
     3361 actactcgtg gacgatcgac aagagcgtcg atccggcgga gcatcacctg ttcgcgggtg
     3421 gcagcgccga ctcggactac gacgtgcttg tggacctgac gtactcggag gacggcttcg
     3481 cgattt
//
LOCUS       DBNHQZ010000146         2572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_854958, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000146 DBNHQZ010000000
VERSION     DBNHQZ010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_854958"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="AC16YU_03820"
     CDS             complement(<1..1322)
                     /locus_tag="AC16YU_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR6064382.1"
                     /translation="MRPRFFSAVPLVALLAAAFIASPASAQESGTAITPEPVWSWDPT
                     DPRIGLKPGVTDAGVAAKGVRLLSNTPLTPEFSAAAGSGGGWVANTDFAFQGDLVFMG
                     NFGGFNVYDYSDPTAPEPVVSVVCPGGQGDLSVHGNLLFMSVETMDGRVDCGTQGTSG
                     PVDPERFRGVRIFDISDVSSPRQVAGVQTCRGSHTHTLVSDPTDPDHVYVYVQGIGPV
                     RPAEELAGCTAEAGSDETALFRIEVIKVPLAAPAEARVVSEPRIFQDYDSGNIQGLWP
                     GGDHGEGSQETSETDQCHDITVNSSLGLAAGACSGNGILLDISDPVNPVRIAEVSDPN
                     FAYWHSATFNNDGTKVIFTDEWGGGGQARCTPTDRPTWGADAIFTLEDGKLDLAGYYK
                     LPVPQTTTENCVAHNGSLIPVPGRDIMAQAWYQGGRSLLDFTDPAHAY"
     gene            complement(1319..2029)
                     /locus_tag="AC16YU_03825"
     CDS             complement(1319..2029)
                     /locus_tag="AC16YU_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIR6064383.1"
                     /translation="MHFRMRALAPAAALLLPLVAACSSPGAVREQATAPPPATSLVQP
                     GAPGQATLAVDSAPQGERPAVHSEADVRFMQGMIAHHAQALVMTAMVPDREPSEGLSL
                     LAQRIEASQKAEIGLMQQWLRRRGEEVPSIDDPHRHHAGHGMLMPGMLTDAQLDEMRA
                     AAGPEFERLFLSYMISHHEGALTMVDELFAAERAAQDVETYRFASDVDADQRMEIRRM
                     RAMLRSADSPDTQNEPHP"
     gene            2160..>2572
                     /locus_tag="AC16YU_03830"
     CDS             2160..>2572
                     /locus_tag="AC16YU_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064384.1"
                     /translation="MTRQNPVIRFALSGAAAGALSTVVFAGVHHALISDIWFSLAPML
                     LAGAVCGSALAVSYALTAESPEFVGWIRFNGVFVALIVVLGVASIVAFEPITTAAAVL
                     AANRPPNELFERSLPLMGAFTVLSTVVVSLVFGRRP"
BASE COUNT          374 a          877 c          893 g          428 t
ORIGIN      
        1 tcgtaggcgt gggcggggtc cgtgaagtcc aggagcgacc tgccgccctg gtaccaggcc
       61 tgcgccatga tgtcacggcc tggcaccggg atgagcgacc cgttgtgcgc gacgcagttc
      121 tcggtcgtgg tctggggaac gggaagcttg tagtagcccg ccaggtccag cttgccgtcc
      181 tcgagcgtga agatcgcgtc cgcgccccag gtgggccggt ccgtgggggt gcaccgggcc
      241 tgcccgcccc cgccccactc gtccgtgaag atcaccttcg tgccgtcgtt gttgaacgtg
      301 gccgagtgcc agtacgcgaa gttcggatcc gacacctcgg cgatccggac cgggttcacg
      361 gggtccgaga tgtcgagcag gatgccgttg cccgagcagg cgcccgcggc gagcccgagc
      421 gaggagttga ccgtgatgtc gtggcactgg tcggtctcgg acgtctcctg cgagccctcg
      481 ccgtggtcgc ccccgggcca gaggccctgg atgttgccgg aatcgtagtc ctggaagatc
      541 cgcggctcgc tcacgacgcg cgcctccgcg ggcgccgcga gggggacctt gatcacctcg
      601 attcggaaca gcgccgtctc gtccgacccg gcttcagccg tgcaccccgc gagctcctcg
      661 gccgggcgca cgggcccgat tccctgcacg tacacgtaca cgtggtccgg gtcggtcggg
      721 tcacttacga gcgtgtgcgt gtgcgagccg cggcacgtct gcacgccggc cacctgccgt
      781 ggcgagctca cgtccgagat gtcgaagatg cgcacgccgc ggaaccgctc cgggtccacg
      841 ggccccgacg ttccctgcgt cccgcagtcg acccggccgt ccatggtctc caccgacatg
      901 aagagcaggt tgccgtgcac cgaaaggtcg ccctggccgc cagggcacac gaccgagacg
      961 accggctccg gggcggtcgg atcggagtag tcatagacgt tgaatccgcc gaagttgccc
     1021 atgaacacca ggtcaccctg gaaggcgaaa tccgtgttcg cgacccagcc gccgcccgag
     1081 ccggcggccg cggaaaactc gggcgtgagc ggcgtattcg agagcagccg aacgcccttc
     1141 gccgccactc ccgcgtccgt aacgcccggc ttcagcccga tgcggggatc cgtcggatcc
     1201 cagctccaga ccggctccgg tgttatggcc gttccggact cctgggcgga ggccggagac
     1261 gcgatgaagg cggctgcgag cagcgcgacc agggggacgg cagagaagaa tcgtggtctc
     1321 atggatgggg ttcgttctgg gtgtctggtg aatcggcgct tcgaagcatg gcccgcatcc
     1381 ggcggatttc catccgctgg tcggcgtcca cgtccgaggc gaaccggtaa gtctccacgt
     1441 cctgggcggc cctctccgcg gcgaaaagct catcgaccat cgtcagcgca ccctcgtggt
     1501 ggctgatcat gtacgaaagg aacagccgct cgaactcggg cccggcggct gcgcgcattt
     1561 cgtcgagctg cgcatccgtc aacatgccgg gcatgagcat gccgtgaccg gcgtggtgcc
     1621 ggtgcggatc gtcgatcgag ggtacctcct cgccccgccg gcgaagccac tgctgcatca
     1681 gcccgatctc ggccttctgc gacgcctcga tccgctgcgc gagcagggag aggccttccg
     1741 acggctcccg gtccgggacc atggccgtca tgaccagcgc ctgggcatgg tgcgcgatca
     1801 tgccctgcat gaagcggacg tcggcctcgg aatgcaccgc ggggcgctca ccctgcggcg
     1861 ccgaatccac cgccagggtc gcctgtcccg gggcgcccgg ctggaccagc gaggtggcgg
     1921 gaggcggagc ggtcgcttgc tcgcgaacgg cgcccggcga ggagcaggcc gcgacgagcg
     1981 gaagcagcag ggcggcggcc ggcgcgaggg cgcgcatccg gaagtgcatg cgggggtcgg
     2041 gttctgggcg aacggatgcc ccaataaccg cgatgacgta gggagttccc ctacggcatc
     2101 acaagagtcg ggttgtgatt caccgaccga agggtttgat tggtgcgctg cccccgacca
     2161 tgacccgtca gaatcccgtc atccgcttcg ccctttccgg tgccgccgcc ggggcccttt
     2221 cgaccgtcgt cttcgcaggc gtccatcacg ccctgatcag cgacatatgg ttctcgctgg
     2281 ctccgatgct gctcgcgggc gccgtgtgcg gcagcgcact cgccgtctcg tacgcgctga
     2341 cggccgagtc cccggaattt gtggggtgga ttcgattcaa cggggttttc gtggcgctga
     2401 tcgtcgtcct cggtgtcgcc tccatcgtgg cattcgagcc catcaccacg gccgcggcgg
     2461 tgctggcggc caatcggccg ccgaacgagt tgttcgagcg ctccctgccg ctcatggggg
     2521 ctttcaccgt gctgtcgacc gtcgtcgtca gcctggtatt cggacggcgg cc
//
LOCUS       DBNHQZ010000147         8392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_993487, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000147 DBNHQZ010000000
VERSION     DBNHQZ010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_993487"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="AC16YU_03835"
     CDS             <1..430
                     /locus_tag="AC16YU_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064385.1"
                     /translation="VEPQTAAIRARWMPLFRKHGVSMLFTGHEHLYEHWVERYVDAEG
                     NHRRIDQVVSGGGGAPLYWYTGEPDLREYRAGAPADSIRITHLVRPGPARGDNPYHYV
                     IVHVDGPDVWMEVIGVEWGSDFAPYRSTRTRLSDTDSFDR"
     gene            554..1183
                     /locus_tag="AC16YU_03840"
     CDS             554..1183
                     /locus_tag="AC16YU_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019295952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064386.1"
                     /translation="MSRFSLLLVLLAAFAIHGTAVAQDEPHAMSFFITGHGPGDGANL
                     GGLEGADAWCDSLAGDDGEWHAYLSAAGVNARDRIGTGPWYNAKGILVASDVEQLHGE
                     NNLTKETQLDELGEVVNGRGDSPNRHDILTGSQLDGTAFPDDGSDHTCADWTSSAEDG
                     SAQVGHHDRQGGGANPTSWNSAHPSRGCSQANLQGTGGDGLFYCFRVSQ"
     gene            1180..3840
                     /locus_tag="AC16YU_03845"
     CDS             1180..3840
                     /locus_tag="AC16YU_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6064387.1"
                     /translation="MTGLHLAQYRIDAELGRGGMGIVYRATDTKLNRTVALKVLPPHA
                     LSSEDDRARFFREAQAAAQLHHPHIATVFQIDEAVPVEEGKAPQPGAEPRLFIAMEFI
                     DGVTVADRIQAGPMPLTEAVSIATQMGEALRTAHAKSIVHRDIKSANVMLTTDGIAKV
                     LDFGLAKTNQSTMLTRQGSTLGTVAYMSPEQARGSDVDGRTDLYALGTVLYEMVAGRL
                     PFAGDYEQAVLYSILNEPPEPLTALRTGVPMQLEWIVNKLLSKEADYRYQTAADLLAD
                     LKSLNLGGSGHSRRSMPAVSHVDTKALPAAGAVLPKWAYAAIAGALVIGVVVTWLLKP
                     APTVDPGPSVRAQIDLGPDTEGLAGVTVSPSGRLLAWSHQFQEVRVRNLESGEQWSIP
                     ESDRTISLRFSPDENWLLLSRTGRIDRVQVRGGRPILVAQLRSNYGSGQWTPDDAILY
                     ESGDAVYAQSLTGGEPRVIVRRDSSHAYDDPFLTSDGRYLVADEFRPGVGHQGLAVFT
                     YPDGERLGMLEISGFESQYLEPGYLVYEQEVGGLMAVPIQLDPPRLLGAPVPIFERGA
                     STQFSLTDQGHLFIGRRYGQGVGGERIFQRVRWDGAVESTAAAPATYDIFAIAPNGPL
                     RVVVEQQAEEGGESLYVVDLESGVSNRLTYGDVGDNPWWHPSGDSVLYVDRSGPGSQL
                     VLRAADGSGAPRILYEEDRVRYTFPSATSAFDRIVFGRVGEADLDIWMLDVASGTAAP
                     LIEEVSDQDRPKISPDGRFVVYQGDQVSGEFRIYMEPTAADGSRWDLSRGLGFDPHWS
                     SDGRSVYFESVSRGVFRVDIDTSGGRISISNPQPVLDMPMRNFVPLPDGSGVVITRGG
                     GGPAPTSAVEVRYVDVILNWTNELRRLVPR"
     gene            3865..6513
                     /locus_tag="AC16YU_03850"
     CDS             3865..6513
                     /locus_tag="AC16YU_03850"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6064388.1"
                     /translation="MIGLHLAQYRIDAELGRGGMGIVYRATDTKLDRTVALKVLPASA
                     LGSEEDRARFYREAKAAAQLHHPNIASVFQIDEAVALAQDQEPGAGAEKRPFIAMEFI
                     DGVTLSDRVRSAPLKLTEAVSIASQVAEALRAAHAKDIVHRDIKSANVMLTKDGVAKV
                     LDFGLAKTAASTQLTRQGSTLGTVAYMSPEQARGQEVDSRTDLYSLGTIFYEMVAGRL
                     PYPGDYEQAVVYGILNEDPEPLTSLRTGVPMGVEWIVNKLLAKRPEDRYQHADELLVD
                     LRTVDLESAGMTRVSRAPAPVAAPTGRARTAGGTMWAIVAVALLIGLVLGAVFGRGLA
                     PRPARDVTYSELRVLSAEFRHPDLSADGRFLVYAGTGGTDEANMVFVYDFRTGETASH
                     AMPTGAGVPTFSPDGSQIAFVSNGQLQIMPVTGGRPIELAPSAASGLAWIGDSDVVYA
                     TPNGERLMRFHTESGQSSSIMERAEGRSLTPLQSLPDGRVLVNRDRGNLDNQDLVILD
                     PETGEVDLVLEDDGFDGRISQSGHLLYSPDARQLFARRIDPGTGAVSGVPWFVGETSL
                     ESTYAVSASGRFVMLAESNYGARDLVRVRRDGSEQLIGVAPLDFEEIRISPDGRRVAA
                     EVNRYNGRYDQVVIVDMSDEPTSQRLTDESSNYEPAWSPDGSRIVYSSEMDARGRTAI
                     VERAADGSGSPRIVVSDADPIGYVDVSRDGRFISYQEDVYGPGGTFVGVYSVADGAEV
                     YRLKEVGTVYRYPKLSPDGRYLAYVKVAGGGEQVWVSSTDGESSLPVTSAGGDMPAWS
                     ADGTTLYFVRGSTIFAVPVSTSGGFRRTGPEREVYRSERSFYYDVMPDGTGFVIAVSR
                     NEALSASVRVIDNFFDVLDRAVPKGR"
     gene            6618..>8392
                     /locus_tag="AC16YU_03855"
     CDS             6618..>8392
                     /locus_tag="AC16YU_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6064389.1"
                     /translation="MTGLHLAQYRIDAELGRGGMGIVYRATDTKLDRTVALKILPAAA
                     LASDDDRARFLREAKAAAQLSHPNVCHVYQVDEAMPLSEGGELVTGQDALRLFIAMEY
                     IEGETVREVVDRGPMKLLDAVSVAGQVAEALKEAHAKQIVHRDIKSANVMLTSDGVAK
                     VLDFGLAKTAASTMLTRQGSTLGTVAYMSPEQARGQEVDGRSDLYSLGTMLYEMVAGR
                     LPFSGEYEQAVLYGIMNEPPEPLTAVRMGVPMELERIVAKLLAKEADYRYQTVGDLLA
                     DLKTLDLSGSGTHRRSVSAQSGAMTKPLRTAALPNWGWGAIAAALALGALGMWLVESG
                     STLEGRSDVMRFEIDLPNAVRSMMPAWSEDGTDLYYIAGAGSEESTEVRRYSIETGES
                     VAVPGTAGASEVTVSPDDRWLAFKDGRTGRYRRAIRSGGEPSDIPGAESPASHGRYGA
                     DGRFYFTDSSWEIASVGEDGDVIVHTRKDSSMLFPVMPLPTADGRSVFFSNWEGLSFS
                     TGTYRLVDADPGFELVTSQYTFVKILENGHALMIPSGPTNQPAAVAPIDTKTGHLLAA
                     AVPVARMDHWGMAVSRQGHLVYRERG"
BASE COUNT         1420 a         2676 c         2896 g         1400 t
ORIGIN      
        1 ggtggagccc cagacggcgg ccatccgggc acgttggatg ccgctcttcc ggaagcatgg
       61 cgtctcgatg ctcttcaccg ggcacgaaca cctgtacgag cactgggtgg agcggtacgt
      121 ggacgcggag ggaaaccacc gtcgcatcga ccaggtcgtg agcggcgggg gcggcgcccc
      181 cctgtactgg tacaccggcg agccggatct gcgggagtac cgggccggcg ccccggccga
      241 ctcgatccgg atcacgcacc tggtccgccc cggccccgcg cgcggcgaca atccctatca
      301 ctacgtgatc gtccacgtgg acgggcccga cgtctggatg gaggtgatcg gggtagaatg
      361 ggggtcggat ttcgccccgt atcggagcac ccgtacgagg ctgtccgaca cggactcttt
      421 cgatcgatga cccggtatcg gctggtttca tgaagccctt gtgaagccgc gggtggggcc
      481 gtttcttgcg tgtcgctgcc tccgcaatca ggtcgcccgg aaccccctga agccgcatcc
      541 aaccgacgga accatgtcac gattctctct cctcctcgta ctgctcgcgg cattcgccat
      601 ccacggcacg gccgtcgcgc aggacgagcc gcacgccatg tcgttcttca tcacgggcca
      661 cgggccgggc gacggcgcca acctgggcgg cctggaaggg gcggatgcat ggtgcgactc
      721 gctcgcgggg gatgacggcg agtggcacgc ctatctgagc gcggcggggg tgaatgcccg
      781 tgaccggatc ggaaccggcc cctggtacaa cgcgaagggt atcctggtgg ccagcgatgt
      841 cgagcagctg cacggcgaga acaacctgac caaggagacg cagctcgacg agctcgggga
      901 ggtggtcaac ggccggggcg actcgcccaa ccggcacgac atcctgaccg gctcgcagct
      961 ggacggaacc gccttccccg atgacggctc ggaccacacc tgcgccgact ggacctccag
     1021 cgccgaagac ggaagcgcgc aggtcggaca ccacgaccgc cagggcgggg gcgcgaatcc
     1081 gacttcctgg aactcggccc acccgtcgcg gggatgcagc caggcgaacc tgcagggaac
     1141 gggtggcgac gggctcttct actgcttccg ggtaagccag tgaccggcct gcacctcgcc
     1201 cagtaccgca tcgacgccga gctcggtcgt ggcggcatgg ggatcgtgta ccgggctacc
     1261 gataccaagc tcaaccgcac ggtcgcgttg aaggtgctgc ccccgcacgc gctgtcgtcc
     1321 gaggatgacc gtgcccggtt cttccgggag gcccaggcgg cggcgcagct gcaccacccg
     1381 cacatcgcca cggtcttcca gatcgacgaa gcggtcccgg tcgaggaagg gaaggcgccc
     1441 cagcccggcg cggagcccag gctattcatt gcgatggagt tcatcgacgg ggtgacggtg
     1501 gccgatcgca tccaggcggg ccccatgccg ctgaccgagg ccgtgtccat cgcgacgcag
     1561 atgggggagg cgctccgcac cgcgcacgcg aagtccatcg tgcatcgcga catcaagagc
     1621 gccaacgtga tgctgacgac cgacggcatt gccaaggtgc tcgatttcgg gctcgccaag
     1681 acgaaccagt cgaccatgct cacacggcag ggttccacgc tggggaccgt ggcctacatg
     1741 agcccggagc aggcgcgcgg aagcgacgtg gacggccgca cggatctgta cgcgctggga
     1801 acggtgctct acgagatggt tgccgggcgg ctgccgttcg ccggggatta cgagcaggcc
     1861 gtgctgtact cgatcctgaa cgagcccccc gagccgctga cggcgctgcg caccggcgtg
     1921 ccgatgcagc tcgagtggat cgtgaacaag ctgctgtcga aggaagcgga ctaccggtac
     1981 cagacagctg cggacctgct ggcggacctc aagtcactga acctgggcgg aagcggccat
     2041 tcgaggcggt cgatgccggc cgtgagccac gtcgacacga aggctttgcc ggccgcgggc
     2101 gccgtgctgc cgaaatgggc atacgccgcg atcgccggcg cgctcgtgat cggggtcgtc
     2161 gtcacgtggc tcctgaagcc ggcacccacc gtcgatcccg ggccgagcgt ccgcgcccag
     2221 atcgacctgg gcccggacac cgagggcctt gccggggtta ccgtatcgcc ctccggccgg
     2281 ctcctggcgt ggtcgcacca gttccaggag gtacgggtcc ggaatctcga atccggtgag
     2341 cagtggtcga ttcccgaatc ggatcgtacg atctcgctgc ggttcagccc ggacgagaac
     2401 tggctgctgc tcagcaggac gggccggatc gaccgcgtgc aggttcgagg ggggcgcccc
     2461 atcctcgttg cccagttgcg atcgaactac gggtcgggac agtggacgcc ggatgacgcc
     2521 atcctctatg agagcggcga cgccgtctat gcgcagtccc tgaccggtgg cgagccacgg
     2581 gtcatcgtgc gccgggattc ttcccatgcg tatgacgatc cgttcctgac ctccgacggc
     2641 aggtacctgg tcgcggacga gttccgcccg ggcgtcgggc accagggact cgccgtgttc
     2701 acctatcccg acggcgaacg gctcggaatg ctcgagatca gcggcttcga atcgcaatac
     2761 ctggaaccgg gttatctcgt gtacgagcag gaggtggggg gcctgatggc cgtcccgatc
     2821 cagctcgatc cgccccgtct tctcggtgcg cccgttccga ttttcgagcg gggcgcatcg
     2881 acccagttct cgctcaccga ccagggtcac ctgttcatcg gccgccgata cggtcagggg
     2941 gtcggtggcg aacggatatt ccagcgcgtg cggtgggacg gagcggtcga atcgaccgcg
     3001 gcagccccgg ccacctacga catcttcgcc atcgctccga atggaccgct tcgtgtcgtg
     3061 gtcgagcagc aggccgagga gggaggcgag agcctgtacg tggtcgacct ggagtccggc
     3121 gtctcgaaca ggctcaccta cggcgacgtg ggagacaatc cgtggtggca tccgtcgggt
     3181 gactcggtcc tctacgtgga ccgctccggc cccggctcac agctcgtgct gcgtgccgcc
     3241 gacggttccg gggcgccccg catcctgtac gaggaggacc gcgtccggta cacgtttccc
     3301 agcgccacct ccgcgttcga ccgtatcgtc ttcgggcgcg tcggggaggc ggatctcgac
     3361 atctggatgc tggacgtagc ctcgggcacc gcggctccgc tcatcgagga agtgtcggac
     3421 caggaccggc cgaaaatctc cccggatggc cgtttcgtgg tgtaccaggg cgaccaggtg
     3481 tccggcgaat tccggatcta catggagccg accgccgcgg acgggagccg ctgggatctg
     3541 tcacgcggcc tcgggttcga tccgcactgg tcatccgatg gccggtcggt gtacttcgaa
     3601 tcggtcagcc gcggcgtgtt tcgggtggac atcgatacga gcgggggacg catctcgatc
     3661 tccaacccgc agcctgttct ggacatgccg atgaggaact tcgtgccgct tcccgacgga
     3721 tcgggcgtgg tgatcacgag gggtggaggc ggacccgctc cgaccagcgc cgtcgaagtc
     3781 cggtacgtgg acgtcatact caactggacc aacgaactcc gacggctcgt accgcgttag
     3841 gcggcgacct tcacccggcc tgccatgatc ggtctccatc tcgcccagta ccgcatcgac
     3901 gccgagctcg gaagaggcgg catggggatc gtgtaccggg ccaccgacac gaagctcgac
     3961 cggacggtgg cgctcaaggt gctgccggcc tcggcgctcg ggagcgaaga agatcgtgcc
     4021 cgtttctacc gcgaggcgaa ggcggcggcc cagctgcacc atccgaacat cgcctccgtc
     4081 ttccagatag acgaggcggt ggccctcgcc caggatcagg agccgggcgc gggagccgag
     4141 aagcgtccgt tcatcgcgat ggagttcatc gacggcgtga cgctgtccga tcgcgttcgg
     4201 agcgccccac tcaaactcac ggaagccgtc tcgatagcgt cccaggtggc ggaggctctc
     4261 agggccgccc acgccaagga catcgtccat cgcgacatca agtccgcgaa cgtcatgctg
     4321 acgaaggacg gcgtggccaa ggtgctcgat ttcgggctgg cgaagaccgc ggcgagcacg
     4381 caactgacgc gccagggctc gacgctgggg acggtggcct acatgagccc cgagcaggcc
     4441 cgggggcagg aagtggactc gcgtaccgac ctgtacagcc tcgggaccat cttctacgag
     4501 atggtggcag ggcgcctgcc gtacccgggc gactacgagc aggccgtggt ctacggcatc
     4561 ctgaacgagg atccggagcc gctgacgtcc cttcgcaccg gcgtgccgat gggtgtcgag
     4621 tggatcgtga acaagctgct cgcgaagcgt cccgaggacc gctatcagca tgccgacgag
     4681 ttgctcgtgg acctgcggac ggtggacctg gagtcggcgg gaatgacgcg cgtatcgcgt
     4741 gctccggcac cggtcgcggc gccgaccgga agagcgagga cggcgggcgg gacgatgtgg
     4801 gcgatcgtcg ccgtcgccct cctcatcggc ctggtgctcg gagcggtgtt cgggcgcggc
     4861 ctggcgcccc ggcccgcacg cgacgtcacg tactccgaac tccgcgtcct ttcggctgaa
     4921 tttcgccatc cggacctctc ggccgacggg cgattcctgg tctatgcggg caccggcggc
     4981 acggacgagg cgaacatggt gttcgtatat gatttccgca cgggagagac cgcttcgcat
     5041 gccatgccga ccggagccgg agtcccgacc ttctcgccgg acggcagcca gatcgcgttc
     5101 gtgtccaacg gccagctgca gatcatgccg gtgacgggcg gacgaccgat cgagctcgca
     5161 ccctcggccg ccagtggact cgcctggatc ggcgacagcg acgtcgtgta cgcgacgccg
     5221 aacggagagc ggctcatgcg tttccacacg gagagcggcc agtcgagttc gatcatggaa
     5281 cgtgctgaag gtcgcagcct gaccccgctc cagagccttc cggacggcag ggtcctggtg
     5341 aacagggacc ggggaaacct tgacaaccag gacctggtca ttctcgatcc ggaaacgggc
     5401 gaggtcgacc tcgtgctcga ggatgacgga ttcgatggcc gaatttccca gtccggccat
     5461 ctgctgtact caccggacgc ccgtcaactc tttgcccgga gaatcgaccc gggaacgggt
     5521 gcggtgtcgg gtgtgccgtg gttcgtcgga gaaacgtcgc tcgagtcgac gtacgccgtg
     5581 tcggcttccg gtcgcttcgt catgcttgcc gaatccaatt acggggcacg ggatctcgtg
     5641 agggtccggc gggacgggag cgagcagttg atcggcgtgg ccccgctcga cttcgaggaa
     5701 atccggatca gcccggacgg caggcgcgtc gcggcggagg tcaaccggta caacggccgg
     5761 tacgaccagg tcgtgatcgt cgacatgtcg gacgagccga ccagccagag attgacggac
     5821 gagtcgtcga actatgagcc ggcctggtcg ccggacggat cgaggatcgt ctactcttcg
     5881 gagatggatg cccgcggaag gacggcaatc gtcgagcggg ccgccgacgg ctcgggctcg
     5941 ccgagaatcg tcgtgtccga tgccgatccc atcggctacg tggacgtgtc ccgggacggg
     6001 cggttcatct cctaccagga ggacgtgtac ggaccgggtg ggactttcgt cggcgtgtac
     6061 tccgtggcgg acggcgcgga ggtgtaccgt ctcaaggagg tcggcacggt atatcgttat
     6121 ccgaagctgt cccctgacgg tcgatacctg gcgtacgtca aggtggcggg cgggggcgag
     6181 caggtatggg tgtcgtccac cgacggcgag tcgtcgctac ccgtcacgtc ggcaggggga
     6241 gacatgccgg cctggtccgc ggatggaacg accctctact tcgttcgtgg atccacgatc
     6301 ttcgcggttc cggtttccac ctcgggcggc tttcgccgca ccggaccgga acgcgaggtc
     6361 tatcggtcgg agcgaagctt ctattatgac gtcatgccgg acggcacggg cttcgtcatc
     6421 gccgtatcac gcaacgaggc cctttcggcc tcagttcgag tgatcgacaa cttcttcgat
     6481 gtgctcgacc gtgcggtgcc gaaggggcga tgaggctagg tatggtctgg aatgctatct
     6541 tgtgaagagg tcgtgaagct accggatgtt tcggggctgt caggctgcca tctgcaaccg
     6601 ggtacacgtt cgcgcccatg accggtctcc acctcgccca gtaccgtatc gatgccgagc
     6661 tcggccgcgg cggcatgggt atcgtctatc gggcgaccga cacgaagctg gaccgcacgg
     6721 tcgctctcaa gatccttccg gcggccgcgc tcgcatcaga cgacgatcga gcccgtttcc
     6781 tgcgagaggc caaggctgcc gcccagcttt cccaccccaa tgtctgccac gtttaccagg
     6841 tggacgaggc gatgccgctc tcggaaggcg gtgagctggt caccggccag gacgccctcc
     6901 gactcttcat cgcgatggag tacatcgagg gagagacggt ccgtgaggtc gtcgatcgcg
     6961 gtcccatgaa gcttctggat gcggtcagcg tggccggtca ggtggccgag gcgctcaagg
     7021 aggcccacgc caagcagatc gtccaccgcg acatcaagtc ggccaacgtg atgctgacgt
     7081 ccgacggcgt ggccaaagtg ctcgacttcg gtttggcgaa gacggccgcg agcacgatgc
     7141 tcacccgcca gggctcgact ctcgggacgg tcgcgtacat gagcccggag caggcgcggg
     7201 gtcaggaggt ggacggccga agtgatctct actccctcgg gacgatgctc tacgagatgg
     7261 tggccggtcg gctacccttt tcgggggagt acgagcaggc ggtgctctac ggcatcatga
     7321 acgagccgcc ggagcccttg acggccgtga gaatgggcgt gccgatggag ctggagcgga
     7381 tcgtggcgaa gctgctcgcg aaggaggcgg actaccgata ccagacggtg ggggacctgc
     7441 tggcggatct gaaaacgctc gatctgagcg ggagcggcac gcaccggcgg tcagtgtcgg
     7501 ctcagagcgg cgccatgacc aagccgttgc gaacggcagc gctgccgaac tggggatggg
     7561 gagccattgc ggcagcgctc gcacttggcg ccctggggat gtggctggtc gagtcaggct
     7621 ccaccctcga aggccgcagc gatgtgatgc gattcgaaat cgaccttccg aacgccgtcc
     7681 ggtccatgat gccggcgtgg agcgaagacg gcaccgattt gtactacatc gccggcgccg
     7741 gttcggaaga atcgacggaa gtgcgacgct attcgatcga gacgggcgag tcggtcgccg
     7801 tgccgggcac cgctggtgcc tccgaggtga ccgtatcacc cgacgataga tggcttgcat
     7861 tcaaggatgg gcgtacgggc cggtatcggc gggcgattcg atcgggcggt gagccgtccg
     7921 acatcccggg tgcagaatcg ccggcctctc atggtcgcta cggagccgat ggacgattct
     7981 acttcaccga ctcgtcctgg gagatcgctt cggtgggaga ggacggcgac gtcatcgtgc
     8041 atacacgaaa ggactcgtcg atgctcttcc ctgtcatgcc attgccgacg gcggacggga
     8101 ggtcggtgtt cttctccaac tgggagggtc tctcgttctc gaccggcacc tatcggctcg
     8161 tggatgcgga tcccggcttc gagctggtca cctcccagta cacgttcgtc aagattctgg
     8221 aaaacggaca cgccttgatg atcccgtccg gtccaacgaa tcagcccgca gccgtcgcgc
     8281 cgatcgatac gaagacaggg catcttctgg ctgcagccgt ccccgttgcc cggatggacc
     8341 actggggaat ggcggtttcg cgccagggac acctcgtgta tcgcgaacgg gg
//
LOCUS       DBNHQZ010000148         6899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_178355, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000148 DBNHQZ010000000
VERSION     DBNHQZ010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_178355"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="AC16YU_03860"
     CDS             complement(<1..988)
                     /locus_tag="AC16YU_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6064390.1"
                     /translation="MTVPVAARAGRPVVAVVFGGQSPEHEVSVISGLQAYAATDTERW
                     DPVPLYLAKDGSWWTGSALGSVDAFVQTDAFRSSGRRVSVRPSPGGRLVLEPERRSRF
                     SRTVLQPVDIVFPVLHGGSGEDGGLQGLCETLGVPYVGSGVMGSAIGMDKEVSKLLGR
                     QAGIPVVDFVAIRESAWADREAEWLDRCESELGWPVIVKPARLGSSIGIARATDRASL
                     DAAIEEAYRYDGKVVVERAVRELREINCSVLGDPDQAVASVLEEPVSSDAVLSFRDKY
                     LKQDTKGRGRGSKARPSEGMASLDRVIPAPVDQAETDAIRDLAVRVFRTFECS"
     gene            complement(985..2271)
                     /locus_tag="AC16YU_03865"
     CDS             complement(985..2271)
                     /locus_tag="AC16YU_03865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6064391.1"
                     /translation="MTPDLLITGGTLVDPESGERRRADILIRNGIIASVGEGLDADDV
                     PRYDASDRLVSPGWMDMHVHLREPGQEYKETIETGCRAAAFGGFTAVACMPNTDPPIH
                     TRDVVEFVVERSEQTAVEVHPIACVSRDRAGRTLTEMADLAAGGAVAFSDDGSPVQDA
                     GLMRRALEYAGMIGRPIINHMEDLGLNDHGQMHEGEISTRLGLAGIPAIAEEVMIARD
                     ILIAGYTGGHIHVAHISTARAVDLVREGKARGVRVTAEACTHHFALTDEAVAESDFDT
                     HTKMHPPLRTAADVEAIREGLRDGTIDAICTDHAPHATFEKEVEFTAAPFGIIGLETA
                     WGLVGRELLATGVLTEAEAVYRLAVAPRRILGLPVPSIAEGQAANLTVFDATTRWTFG
                     EEHIRSKSRNTPFIGAPMVGRAFATYHRARLSEATP"
     gene            2442..3554
                     /locus_tag="AC16YU_03870"
     CDS             2442..3554
                     /locus_tag="AC16YU_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6064392.1"
                     /translation="MRRSFLLLVTLLAAAPALARQDGAPDPVQTADGVRLSLSAVIQT
                     RASYGISDTGANEANRLGVGIRRARFRARADFGTGAGLYMQIEGAGGTLTFLDFYGRM
                     RLAERWTLRMGRFASAQPRGFILTPIHFIDVADRAAVVERWGQATVGGDGRDFGIEAA
                     YRAGGTDVLFFLHNGDGSWSRIRGNFRPSISEGSATDGVDRTGLAATASLSHRPASRP
                     DVEIGGFAGYNGAEGPNADGRSYGTYGVHAYLGALPGSRPLRLKLDLLGISYEERPFE
                     SEAQNSLGASLLAAALAAPALEVFGRVEVFTADLRDEAPTGDDLYLTLGATFSPSARR
                     GGEFHRERITLAWQRVDFGDESGTEDLVFVQLQLGF"
     gene            3554..4681
                     /gene="alr"
                     /locus_tag="AC16YU_03875"
     CDS             3554..4681
                     /gene="alr"
                     /locus_tag="AC16YU_03875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6064393.1"
                     /translation="MALLPPSDPASVVAHVDLGALGHNLGVLRDRAAGLDVMAVVKAN
                     AYGHGAVPVTRFLAGEGVSRFGVATLGEAVALREAGIRERILVFAAPVPAQLERYARH
                     DLEVTVTSPEVAREVARIATAADPVRVHVKVDTGMGRIGLDPADVVDTVRLLERSEGV
                     RIEGFWTHFATADEPDDTFAREQWTRFRRVLTDLGGAPAPVHAANSGGLLGNAVALDP
                     ELVSGVRLGISLYGLYDPPDRALRPVMSLVSRLTQVKTVPTGTPISYGATWRAPRETR
                     IGTVSAGYADGYRRALSNRSEVGVAGRRCPIAGRVCMDMIMTDLGPGGEGPPAAEGDE
                     VVLFGTGGPSAFEVARWADTITYEIVCGITERVTRRYGEAV"
     gene            4792..5502
                     /locus_tag="AC16YU_03880"
     CDS             4792..5502
                     /locus_tag="AC16YU_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6064394.1"
                     /translation="MASTFDPSQLNILVVDDEEDVAEVVSHFLRQEGFNVQVAYDGND
                     ALAKATPDVDLVVLDIMLPGMDGYEICRRLRSRVETETIPIVFLTAKAEEDDQVKGLM
                     LGADAYLTKPVSPQVIVAHVKAVLRRSGIEESRTLQVRNLTIYEDEYRASLDGRDLGL
                     TLTEFELVRYLVRHPRKAFTRQQLLETIWKDAMMVTERTVDAHIKNLREKLGSFAQHI
                     QTVRGVGYRFVEEGAAQE"
     gene            5555..>6899
                     /locus_tag="AC16YU_03885"
     CDS             5555..>6899
                     /locus_tag="AC16YU_03885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064395.1"
                     /translation="MARLGRVPARIAGWLFPTRASTQTWMMLTFALFIGVVVVAVGLY
                     LSVEVGSELRTTVEQMLRRQVVLTAESLERAGGDAGLVEEAIAIARVTEDRITVLRGD
                     SVLVDVQHTRLLSDGGILARDEIAGLGNGGFGFAERVEGGVRTYFAAVHRPASGLVVR
                     VGMPESALVLLVHQLQVIIAAAMLLVFLLSVVAAWIAARRVTEPLEGIRRSARHIAGG
                     QLEQDIVVESRAAEFQDLAASLNRMSDTFKDRIGDLQRLAALQNEFIGNVSHEVRNPI
                     FAVGGYLEALASSALSADQRRFYAEKGLANLERLNNLFNDLIEIARLEYREDMIKTVD
                     FDLHELVEEVADMLRSKAGDKGLVLVTDNPVVWTHADRNRIRQVLVNLIDNAIAYSDE
                     GLVRCRYRRHLDKVRIEVVDTGRGIPEEHLERIFERFYRVDPDRSRKSGGTGLGLS"
BASE COUNT          981 a         2499 c         2403 g         1016 t
ORIGIN      
        1 ccgagcactc gaacgtgcgg aacacccgga cggcgaggtc gcggatcgcg tccgtctccg
       61 cctggtccac gggggcggga atgacgcggt ccagcgaggc catgccctcg gaggggcgcg
      121 ccttggagcc gcggccgcgc cccttcgtgt cctgcttcag gtacttgtcc cggaacgaga
      181 ggacggcgtc cgacgagacc ggctcctcga gcacgctcgc cacggcctgg tccgggtcgc
      241 cgagcacgga gcagttgatc tcgcgcagct cgcggacggc gcgctcgacg accaccttcc
      301 cgtcgtagcg gtaggcctcc tcgatcgcgg cgtcgagcga cgcgcggtcg gtcgcccggg
      361 cgatgccgat ggacgacccg aggcgcgccg gcttcacgat gaccggccag ccgagctccg
      421 actcacagcg atccagccac tcggcctcgc ggtcggccca cgccgactcc cggatcgcca
      481 cgaaatccac gaccggtatc ccggcctgcc ggccgaggag cttcgacacc tccttgtcca
      541 tcccgatcgc ggagcccatg acgccgctcc ccacgtaggg gacgccgagc gtctcgcaca
      601 ggccctgcag gccgccgtcc tcgcccgaac cgccgtgcag cacgggaaag acgatgtcca
      661 ccggctgcag caccgtccgg gagaagcggc tccggcgctc gggctccaaa accaggcggc
      721 cgccgggcga cgggcgcacc gaaacgcggc gccccgacga gcggaaggcg tcggtctgga
      781 cgaaggcgtc caccgagccg agggcggatc ccgtccacca ggagccgtcc ttcgccaggt
      841 agagcggtac cggatcccag cgctccgtgt cggtcgccgc gtaggcctgc agccccgaga
      901 tcaccgagac ctcgtgctcg ggcgactgcc cgccgaagac gacggccacg acgggacggc
      961 ccgcgcgcgc tgccacgggc accgtcacgg cgtggcctcc gagagccgtg cgcggtggta
     1021 ggtcgcgaag gcccgtccga ccatcggggc gccgatgaac ggcgtattcc gcgacttgga
     1081 gcggatgtgc tcctcgccga aggtccagcg cgtggtcgcg tcgaaaacgg tcaggttggc
     1141 cgcctggccc tcggcgatcg agggcacggg cagcccgagg atgcggcgcg gcgccacggc
     1201 cagccggtac acggcctcgg cctcggtcag gacgcccgtg gcgaggagct cgcgcccgac
     1261 aagtccccag gcggtctcga ggccgatgat gccgaaaggc gccgccgtga actcgacctc
     1321 cttctcgaac gtggcatgcg gcgcgtggtc ggtgcagatg gcgtcgatcg tgccgtcacg
     1381 cagcccttcc ctgatggcct cgacgtccgc cgccgtgcgc agcggcgggt gcatcttcgt
     1441 gtgggtgtcg aagtcgctct cggccacggc ctcgtcggtg agcgcgaagt ggtgcgtgca
     1501 ggcctcggcg gtcacccgca cgccgcgggc cttgccctcg cgcaccaggt ccaccgcccg
     1561 ggccgtcgag atgtgggcga cgtggatgtg tccgcccgtg tacccggcga tcaggatgtc
     1621 gcgggcgatc atgacctcct cggcgatcgc ggggatgccg gccagtccca gccgggtcga
     1681 gatctcgccc tcgtgcatct ggccgtggtc gttcagcccc aggtcctcca tgtggttgat
     1741 gatcggccgg ccgatcatcc cggcgtactc gagggcccgc cgcatgaggc cggcatcctg
     1801 caccggcgag ccgtcgtcgc tgaacgcgac ggcgcctccg gccgcgaggt cggccatctc
     1861 ggtgagggtc ctgccggcgc ggtcccggga gacgcaggcg atcgggtgca cttcgacggc
     1921 cgtctgctcg gagcgctcga cgacgaactc gaccacgtcg cgggtgtgga tcggcggatc
     1981 ggtgttcggc atgcatgcca ccgccgtgaa tccgccgaac gcggcggccc ggcagccggt
     2041 ctcgatcgtc tccttgtatt cctgccccgg ctcgcgcagg tgcacgtgca tgtccatcca
     2101 gccgggcgag acgaggcgat ccgacgcgtc gtaccggggc acgtcgtccg catcgagccc
     2161 ctccccgacc gacgcgatga tcccgttccg gatcaggatg tccgcgcggc gccgctctcc
     2221 tgactcgggg tcgacgaggg tgccgccggt gatgaggaga tcgggtgtca tcgcagggtt
     2281 cggatcgatc ggagtggccg cctcccggca atatagcgcg acgcccggac cggcctgcga
     2341 ctccccgggg cacagttcgc cgtttctttg cgccccggat gtggcgattc gacccggaac
     2401 ccgtttcctt tggcgcacca ccgatccgga cggacccagc catgcgccgc agcttcctgc
     2461 tcctcgtcac cctcctcgcc gccgcaccgg ccctggcccg gcaggacggc gcgcccgacc
     2521 cggtccagac cgccgacggc gtccgcctgt cgctctcggc ggtgatccag acccgggcct
     2581 cgtacggcat ctcggacacc ggcgcgaacg aggcgaaccg gctcggcgtc ggcattcggc
     2641 gcgcccggtt ccgcgcgcgg gcggacttcg gaacgggggc cgggctctac atgcagatcg
     2701 aaggggccgg cggcacgctc accttcctcg acttctacgg ccggatgcgg ctcgccgagc
     2761 ggtggaccct gcgcatgggc cgcttcgcgt ccgcgcagcc ccgcggcttc atcctgaccc
     2821 ccatccactt catcgacgtg gcggaccgcg cggccgtcgt ggagcgctgg ggccaggcga
     2881 ccgtcggcgg ggacggccgc gacttcggca tcgaggccgc gtaccgtgcg ggcggcacgg
     2941 acgtgctctt cttcctgcac aacggggacg gcagctggag ccggatacgg ggcaacttcc
     3001 gaccgtccat ttccgaggga tcggccacgg acggcgtgga tcggaccggg ctcgccgcga
     3061 cggcgtccct gtcgcaccgc ccggcgtcgc ggcccgacgt cgagatcggc ggcttcgcgg
     3121 gctacaacgg ggccgagggg cccaacgccg acggccgctc ctacggcacg tacggcgtcc
     3181 atgcgtacct gggcgccctg cccggctcgc ggccccttcg gctgaagctg gatctcctcg
     3241 gcatctcgta cgaggaacgt ccgttcgaat ccgaggcgca gaactcgctc ggcgcctcgc
     3301 tgctggcggc cgccctcgcc gccccagcgc tcgaggtctt cggaagggtc gaggtcttca
     3361 cggccgacct gcgcgacgag gccccgacgg gtgacgacct gtacctgacg ctcggcgcca
     3421 cgttcagccc gtcggcgcgg cgcggcggcg agttccaccg cgagcggatc acgctcgcct
     3481 ggcagcgggt ggacttcggc gacgagagcg gcacggagga cctcgtcttc gtgcagctgc
     3541 agctcggctt ctgatggctc tcctgccgcc gtccgacccg gcctccgtgg tcgcccacgt
     3601 ggacctgggc gccctcggcc acaatctcgg cgttctgcgc gaccgggccg ccggcctgga
     3661 cgtgatggcc gtcgtgaagg ccaatgcgta cgggcacgga gccgtcccgg tcacgcggtt
     3721 cctcgccggg gagggcgtct cgcgcttcgg cgtggccacg cttggcgagg ccgtcgccct
     3781 gcgcgaggcg ggcatccggg agcgcatcct ggtgttcgcg gcccccgtcc ccgcgcagct
     3841 ggagcggtac gcgcggcacg acctcgaggt gaccgtgacc tcccccgagg tcgcccgcga
     3901 ggtcgcccgc atcgcgaccg ccgccgatcc cgtccgcgtg cacgtcaagg tggacacggg
     3961 catggggagg atcggcctgg atccggcgga cgtcgtggac accgtccggc tcctggagcg
     4021 ctccgagggc gtgcgcatcg agggcttctg gacccatttc gcgaccgccg acgaacccga
     4081 cgacacgttt gcgcgcgagc agtggacccg cttccgccgg gtgctcacgg acctcggcgg
     4141 cgcacccgcg ccggtgcacg ccgcgaattc cggcggcctc ctgggaaacg cggtcgccct
     4201 cgaccccgag ctcgtgtcgg gcgtccgcct gggcatctcg ctctacgggc tgtacgatcc
     4261 gcccgatcgc gccctgcggc cggtcatgag cctcgtttcg cggctcacgc aggtcaagac
     4321 ggtccccacg ggcacgccca tttcgtacgg cgccacctgg cgcgcgcccc gcgaaacccg
     4381 gatcggcacc gtctcggccg gctacgcgga cggatacagg cgggcactct cgaaccgctc
     4441 cgaggtcggc gtcgccggcc ggcggtgtcc gatcgccggc cgcgtgtgca tggacatgat
     4501 catgaccgac ctcgggccgg gcggcgaggg tcccccggcc gcggagggcg acgaggtcgt
     4561 gctcttcggc acggggggac cgagcgcctt cgaggtggcc cgctgggccg acaccatcac
     4621 gtacgagatc gtgtgcggca tcacggaaag ggtcacccgt cgctacggcg aggcggtctg
     4681 acgggacgcg cgcttccgtt cctcgcggcc ggcttgtgac ttgcaggtga agtgtatatt
     4741 ccccgctcct gcaggcgaag cccgcgccca accaaaccca gcgcaccgat catggccagc
     4801 accttcgacc cgtcccagct gaacatcctt gtcgtcgacg acgaagagga cgtcgccgaa
     4861 gtcgtctccc atttcctgcg ccaggaggga ttcaacgtcc aggtcgcgta cgacggcaac
     4921 gatgcgctgg cgaaggcgac gcccgacgtg gacctcgtcg tgctcgacat catgcttccg
     4981 ggcatggacg ggtacgagat ctgccggcgc ctgcgctcgc gggtcgagac cgagaccatc
     5041 ccgatcgtct tcctgacggc gaaggccgaa gaggacgacc aggtgaaggg cctcatgctc
     5101 ggagcggacg cctacctgac caagccggtg tccccgcagg tgatcgtggc ccacgtgaag
     5161 gcggtcctgc gccgctcggg catcgaggag agccgcaccc tccaggtgcg caacctcacg
     5221 atctacgagg acgagtaccg cgcctcgctc gacggccgcg acctggggct gacgctcacc
     5281 gagttcgagc tcgtgcgcta cctcgtgcgg cacccccgca aggcgttcac acggcagcag
     5341 ctgctcgaaa ccatctggaa ggacgccatg atggtcaccg aacgcaccgt ggacgcccac
     5401 atcaagaatc tccgggagaa gctcggttcg ttcgcgcagc acatccagac ggtgcgcggc
     5461 gtcgggtacc ggttcgtcga ggagggcgcc gcccaggagt agcgtccgcc ccccggcgac
     5521 ctcacgccgg acccctcccg caccccgttc ccccatggcc cggcttggtc gcgtcccggc
     5581 ccgcatcgcc ggctggctct ttccgacccg cgcgtccacg cagacgtgga tgatgctcac
     5641 gttcgcgctg ttcatcggcg tggtggtcgt ggcggtcggc ctgtacctct cggtcgaggt
     5701 cggctccgag ctccgcacga cggtcgaaca gatgctccgg cgtcaggtgg tcctcacggc
     5761 cgaaagcctg gaacgcgccg gcggcgacgc cggcctcgtc gaggaggcga tcgcgatcgc
     5821 gcgcgtcacc gaggaccgga tcacggtgct gcgcggcgac agcgtgctcg tcgacgtgca
     5881 gcacacgcgc ctgctctcgg acgggggcat cctcgcccgg gacgagatcg cgggcctggg
     5941 caacgggggc ttcggattcg ccgagcgggt cgagggcgga gtccggacgt acttcgcggc
     6001 cgtgcaccgg ccggcctcgg ggctcgtggt ccgggtgggc atgcccgagt ccgccctggt
     6061 actcctggtg caccagctcc aggtgatcat agccgcggcg atgctactcg tgttcctgct
     6121 ctcggtggtc gcggcgtgga tcgcggcccg gcgggtcacc gagccgctcg agggcatccg
     6181 gcgcagcgcc cggcacatcg ccggcgggca gctcgaacag gacatcgtgg tcgaatcgcg
     6241 ggcggctgaa ttccaggacc tggccgccag cctgaaccgg atgtcggata cgttcaagga
     6301 ccgtatcggc gacctgcagc ggctcgccgc gctccagaac gagttcatcg gcaacgtttc
     6361 gcacgaggtc cgcaatccga ttttcgccgt gggcggctac ctggaggcgc tcgcctcgtc
     6421 cgccctgtcc gccgatcagc gccgcttcta cgcggagaag gggctcgcga acctggagcg
     6481 gctgaacaac ctgttcaacg acctgatcga gatcgcacgt ctggaatacc gcgaggacat
     6541 gatcaagacg gtcgacttcg acctgcacga gctcgtcgag gaagtggccg acatgctccg
     6601 gtccaaggcg ggagacaagg gcctggtgct tgtcacggac aaccccgtgg tctggacgca
     6661 cgccgaccgc aaccgcatcc gccaggtcct ggtgaacctg atagacaacg cgatcgcata
     6721 ctcggacgag ggcctcgtgc ggtgccgcta ccgccggcac ctggacaagg tgcgcatcga
     6781 ggtggttgac acgggccgag gaatccccga ggagcacctc gagcgcatct tcgagcgctt
     6841 ctaccgggtc gatcccgacc ggtcccggaa gagcggcggc accgggctcg gcctgtcga
//
LOCUS       DBNHQZ010000149         2229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1127079, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000149 DBNHQZ010000000
VERSION     DBNHQZ010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1127079"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="AC16YU_03890"
     CDS             complement(<1..636)
                     /locus_tag="AC16YU_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR6064396.1"
                     /translation="MSERPPSDCPYPDSAGPLSRRAFAKAMGVLALGTQLPGCFGGDG
                     EDTSAHGPFVPQAGPGGMTVLSDLRVPMRDGVHLATDVYLPAVDGEALPGPFPVILER
                     TPYGKTRPSRSERSRAEPDEAKGRAEVARMFVDRGFAVIYQDNRGRYDSEGTYTKYLD
                     NVEDGYDCCVWILEQPWCDGQIGTMGLSYAAHTQAALASAGAPGVASMFIDS"
     gene            complement(982..1479)
                     /locus_tag="AC16YU_03895"
     CDS             complement(982..1479)
                     /locus_tag="AC16YU_03895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6064397.1"
                     /translation="MHCKTLRSSFFARALPLVFVTLAASGCLQDEPAGPSVDYQVIEE
                     VTFAESLGVDLDAMSVTSEGIYIQDLAEGDGPEADFGDLVEIVYSGYLNTGVQYLPED
                     TIQFQLGGEIVPEGVHLAMLGQQAGGQRLMVIPPRYGYGSRSYENVPAGSILIFRVEL
                     LELIG"
     gene            1621..>2229
                     /locus_tag="AC16YU_03900"
     CDS             1621..>2229
                     /locus_tag="AC16YU_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase family
                     protein"
                     /protein_id="HIR6064398.1"
                     /translation="MSEKLYDVAILGGGNLGEALARGWANAGSFDAGRIVVTRRHPDK
                     LAALSAEGFVVTDSNADAVRGANLIVLAVQPQQLGALLDEIGPSLESGRHRLISVVSG
                     ASIDEIRKAAGGTSVPIARAMPNTAVMIGESMTCLAGEEGGNADVLREAEELFAVVGR
                     TLVIPEDMIVPATALCACGIAFFLRCVRAAAQGGIEIGFHPED"
BASE COUNT          361 a          768 c          763 g          337 t
ORIGIN      
        1 cgaatcgatg aacatcgacg ccaccccggg cgcgcccgcc gaggccagcg cggcctgggt
       61 gtgcgcggcg tacgagagtc ccatcgtccc gatctgcccg tcgcaccagg gctgctcgag
      121 gatccacacg cagcagtcgt atccgtcctc gacgttgtcg aggtacttcg tgtacgtccc
      181 ttccgagtcg taccgtccgc ggttgtcctg gtagatgacc gcgaagccgc gatcgacgaa
      241 catgcgcgcg acctcggccc gccccttcgc ctcgtcgggc tccgcgcgcg accgctcgct
      301 ccggctcggg cgcgtcttgc cgtagggcgt ccgctccagg atgactggaa acggccctgg
      361 gagggcctcg ccgtccacgg ccggcagata gacgtcggtc gcgaggtgga caccgtcgcg
      421 catggggacc cggaggtccg acagcaccgt catccctccc ggacccgcct gcggcacgaa
      481 cggcccatgg gccgacgtgt cctccccgtc gcccccgaaa cagcccggaa gctgcgtgcc
      541 gagcgcgagg acgcccatcg ccttggcgaa ggcgcgccgc gagagtgggc ctgccgagtc
      601 cggataagga cagtccgaag gtggacgctc gctcaaggaa tcaactccgg ctggggtggg
      661 gtgagcggcg gatattagcc ctcgtcgtcc cggcgcgcat cctcaccgag ggctcgagtt
      721 tgatcttctc cccgccaggt tgaaactcgt tcgagtttcg aacgatctct gccgttacgg
      781 ctgcgcggac gccgcctcca tggcccaggc acggcgcggg ctagctcgac cgggcagcca
      841 gccgagctac cttcgaactc gttcgacttt cgaacgactt tcgaatggcc ggcagaagac
      901 accgcacccc gcacccaagc ccaggcccaa gcgcacgcgc acgcgcacgc gagtcagaag
      961 tcctggtggg agaacggcca gctagccgat cagctccagc aactcgaccc ggaagatcag
     1021 gatcgacccg gccgggacgt tctcgtacga ccgcgagccg tacccgtacc gcggcgggat
     1081 caccatgagg cgctggccac ccgcctgctg cccgagcatc gccaggtgca cgccctcggg
     1141 cacgatctcc ccaccgagct ggaactggat ggtgtcctcc ggcaggtact ggacgccggt
     1201 gttcaggtag ccactgtaga cgatctcgac cagatcgccg aagtcggcct cagggccgtc
     1261 gccttcggcg agatcctgga tgtagatgcc ttcgctggtc acggacatcg cgtcgaggtc
     1321 gacgccgagg ctctcggcga acgtgacttc ctcgatgacc tggtagtcca ccgagggacc
     1381 ggcgggctcg tcttgcaggc aaccggaagc agcgagggtc acgaagacga gcggaagggc
     1441 gcgcgcgaag aatgacgaac gaagtgtctt gcagtgcata ggcgaggctc cgtgtgacgt
     1501 tgcgatcgat tacgttagcc acgaacccgg gaggcacgcg aggggcgtgc cgcccggcga
     1561 cgactccgaa tacaccgcgc ggcggcccgc tatccgtccg cgcatcctga agggggatcc
     1621 gtgagcgaga agctgtatga cgtggccatc ctgggcggcg gcaatctggg cgaggcgctg
     1681 gccaggggct gggcgaacgc gggctcgttc gacgcgggcc gcatcgtcgt gacgcgccgc
     1741 catcccgaca agctggcggc gctctccgcc gagggcttcg tggtgaccga ctcgaacgcc
     1801 gacgcggtac ggggggcgaa cctgatcgtc ctggcggtgc agccacagca gctcggcgcc
     1861 ctcctggacg agatcgggcc ctctctcgag tccgggcggc atcggctgat ctccgtcgtg
     1921 tcgggggcct ccatcgacga gatccggaag gccgcgggcg gcacgtcggt ccccatcgcg
     1981 cgcgccatgc cgaacaccgc ggtgatgatc ggtgagtcga tgacctgcct ggcgggcgag
     2041 gagggcggca acgccgacgt gctccgcgaa gccgaggagc tgttcgccgt ggtcgggcgt
     2101 accctggtca tcccggagga catgatcgtc cccgcgaccg cgctctgcgc ctgcggcatc
     2161 gcgttcttcc tccgctgcgt acgggcggcg gcacagggcg gcatcgagat cggcttccat
     2221 ccggaggac
//
LOCUS       DBNHQZ010000150         4435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3431521, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000150 DBNHQZ010000000
VERSION     DBNHQZ010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3431521"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC16YU_03905"
     CDS             complement(<1..828)
                     /locus_tag="AC16YU_03905"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR6064399.1"
                     /translation="MHAKPDRFTLEQYGISVVEIIRNASPALLYEHALLHDPGSAISD
                     HGALMIRSGAKTGRSPSDKRIVDMPQSHDEVWWGKVNVPLDEHTFEINRERAVDYLNT
                     CDRLYVTDGYAGWDPAYRVKVRVIASRPYHALFMRNMLIRPTARELAAFGDPDYVIYN
                     AGRFPANRHTSHMTSKTSVDLCFERREFVILGTEYAGEMKKGIFTVMHYIMPRQGVLS
                     MHCSANEGDTGDVTLFFGLSGTGKTTLSADPARNLIGDDEHCWTDDGVFNIEGGCYAK
                     "
     gene            complement(885..2861)
                     /gene="acs"
                     /locus_tag="AC16YU_03910"
     CDS             complement(885..2861)
                     /gene="acs"
                     /locus_tag="AC16YU_03910"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6064400.1"
                     /translation="MQQQTSHEPDPALGPLYQARAAFRRRSHLPTMEDYQALYRRSID
                     DPTGFWGEQGKRIHWFEPYHTVHDTSFASSNVDIKWYLGGKTNASYNCLDRHLETKGH
                     RTALIFEPDDPGENPRDLTYRELYEEVCRFANVLKAKGVRKGDVVTIYMPMIPEAAAA
                     MLACARIGAIHSVVFAGFSPDALADRILDGNSKIVVTADEAMRGGRGVHLKQATDRAL
                     ERCPDVKSVIVVRRTGSPVPWTDGRDTWYHEAREKVYAECPCEWMDAEDPLFILYTSG
                     STGQPKGVLHTTGGYLVFTSLTHQYVFDYNDKDVFWCTADIGWITGHSYIVYGPLLNG
                     ATQVFFEGTPHYPDPSRFWEVVDAHGVNQFYTAPTAIRALMRQGEEFVRKTSRHSLRL
                     LGTVGEPINPEAWRWYHDVVGEGRCPIVDTWWQTETGGILITPLPAAIPQKPGSATVP
                     FFGIEPDIVDQDGKRIEGEGMGLLVLNRSWPGQARTIYGDHDRFVKTYFSKFEGRYFT
                     GDGCRRDEDGYYWITGRVDDVINVSGHRIGTAEIESALVGAAGVAEAAVVGFPHEIKG
                     QGIYCFVTLKEGVEPTDQVRRALIHSVREHIGPIVTPDFIHFTESLPKTRSGKIMRRI
                     LRKIAADDFEDLGDTSTLADPSVVDELIKNRRNR"
     gene            3069..3884
                     /locus_tag="AC16YU_03915"
     CDS             3069..3884
                     /locus_tag="AC16YU_03915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6064401.1"
                     /translation="MARFRLDNRLVLVTGAARGMGLGLAHEFAARGARLVLWDVDGPA
                     LVGAAAGLEREGLRVRTDTVDLLDRDAVSAAALRVLTEDGPVDVLVNNAGVVTGRPLL
                     ELSDSEIERTFGVNTLALFWTTRAFLPAMIERGAGHVVTVASAGGLVGIPRLVDYCAS
                     KFAAVGFDEALRVELSTSGHCIGTTVVCPIYVDTGMFEGARTRVPWLLPVLRPEVVVR
                     RTVRAVERGRSRVIIPRFAYSAFPLRLLPVAWFDRIMAWMGLTRSMEGFRGRG"
     gene            4226..>4435
                     /locus_tag="AC16YU_03920"
     CDS             4226..>4435
                     /locus_tag="AC16YU_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6064402.1"
                     /translation="MPTFIPRILEAHLRSLASRHPIVGIVGPRGVGKTVMAHRVFSRL
                     DFVRCDSPEVSRALMEDPAGFIRGFR"
BASE COUNT          624 a         1511 c         1513 g          787 t
ORIGIN      
        1 cttcgcatag cacccgccct cgatgttgaa gacgccgtcg tccgtccagc agtgctcgtc
       61 gtcgccgatc aggttgcggg cgggatccgc gctgagcgtg gtcttgccgg tccccgagag
      121 accgaagaac agcgtgacat cgcccgtgtc cccctcgttc gccgagcagt gcatggacag
      181 gacgccctgc ctgggcatga tgtagtgcat caccgtaaag atgcccttct tcatctcgcc
      241 cgcgtactcg gtgcccagga tcacgaactc ccgccgctcg aagcacaggt ccacgctggt
      301 cttggacgtc atgtgggacg tgtgccggtt cgccgggaag cggcccgcgt tgtagatcac
      361 gtagtccggg tcgccgaacg cggccagttc cctggccgtc ggccggatga gcatgttgcg
      421 catgaacagc gcgtggtacg gccgggacgc gatgacgcgc accttcaccc ggtaggccgg
      481 atcccatccc gcgtagccgt ccgtgacgta caggcggtcg caggtgttca ggtagtcgac
      541 ggcccgctcc cggttgatct cgaacgtgtg ctcgtcgagc gggacgttca ccttgcccca
      601 ccacacctcg tcgtgtgact gcggcatgtc cactatccgc ttgtccgacg ggctccgccc
      661 cgtcttcgca cccgaacgga tcatgagcgc gccgtggtcg gaaatcgccg agcccgggtc
      721 gtgaagcagc gcatgctcgt agagcagggc cggcgaggcg ttccggatga tctcgacgac
      781 ggagatgccg tactgctcca gggtaaatcg gtcgggcttg gcgtgcatgg gagtggaagg
      841 aacggaaatg ggggcatcgg gggcgcgtca acggcctcgc gggttcaccg gttgcgccga
      901 ttcttgatca gttcgtcgac caccgacgga tctgcgagcg tcgacgtatc gccgaggtcc
      961 tcgaagtcgt ccgccgcgat cttgcgcagg atgcggcgca tgatcttgcc gctgcgcgtt
     1021 ttcgggagtg actccgtgaa gtggatgaag tccggcgtga cgatcgggcc gatgtgctcc
     1081 cggaccgagt ggatcagggc gcgccgcacc tggtcggtcg gctccacccc ttccttcagc
     1141 gtcacgaagc agtagatgcc ctgccccttg atctcgtgcg ggaagccgac gacggccgcc
     1201 tcggccacgc ccgccgcccc gacgagcgcg ctctcgatct cggccgtgcc gatccggtgg
     1261 cccgacacgt tgatcacgtc gtccacgcgg ccggtgatcc agtagtagcc gtcctcgtct
     1321 cggcggcatc cgtcacccgt gaagtaccgc ccctcgaact tcgagaagta cgtcttcacg
     1381 aagcggtcgt ggtccccgta gatggtccgg gcctgcccgg gccacgaccg gttcaggacc
     1441 aggaggccca tcccctcccc ctcgatccgc ttcccgtcct ggtccacgat gtccggctcg
     1501 atgccgaaga agggcacggt cgcgctcccc ggcttctgcg ggatggccgc cggaagcggc
     1561 gtgatcagga tgcccccggt ctcggtctgc caccacgtgt cgacgatcgg gcagcggccc
     1621 tcgcccacca cgtcgtggta ccagcgccag gcctccgggt tgatcggctc gcccacggtg
     1681 ccgagcaggc gcagcgagtg ccggctcgtc ttgcgcacga actcttcgcc ctgccgcatg
     1741 agcgcccgga tggccgtcgg cgccgtgtag aactggttca cgccgtgcgc gtcgaccacc
     1801 tcccagaagc gcgaggggtc cgggtagtgc ggcgtgccct cgaagaagac ctgggtggcg
     1861 ccgtttagca gcggcccgta cacgatgtac gagtgcccgg tgatccaccc gatgtcggcg
     1921 gtgcaccaga agacgtcctt gtcgttgtag tcgaatacgt actggtgggt gagcgacgtg
     1981 aagaccaggt acccaccggt cgtgtgcagc acgcccttgg gctgtcccgt ggagccgctc
     2041 gtgtacagga tgaagagcgg gtcctcggca tccatccatt cgcacgggca ctcggcgtac
     2101 accttctcgc gcgcctcgtg ataccacgtg tcgcggccgt cggtccacgg cacgggcgag
     2161 cccgtccggc gcaccacgat cacgctcttc acgtccgggc agcgctccag cgcccggtcg
     2221 gtcgcctgct tcaggtgcac gccccgcccg ccgcgcatcg cctcgtcggc cgtcacgacg
     2281 atcttcgagt tgccgtccag gatgcggtcc gcgagggcgt ccggcgagaa gccggcgaac
     2341 accaccgagt ggatcgcccc gatccgcgcg cacgccagca tggcggccgc cgcctcggga
     2401 atcatgggca tgtagatggt caccacgtcg cccttgcgca cgcccttcgc cttcaggacg
     2461 ttcgcgaacc ggcacacctc ctcgtacagc tcccggtacg tgaggtcacg cgggttctcg
     2521 cccggatcgt ccggctcgaa gatgagcgcc gtccggtgcc ccttcgtctc gaggtggcgg
     2581 tccaggcagt tgtacgaagc gttcgtcttg ccgcccaggt accactttat gtccacgttc
     2641 gacgaggcga acgacgtgtc gtgcaccgtg tggtacggct cgaaccagtg gatgcgcttt
     2701 ccctgctcgc cccagaatcc cgtggggtca tcgatcgacc gccggtacag ggcctggtaa
     2761 tcctccatgg tgggaaggtg cgaacggcgc cggaatgccg cacgggcctg gtacagggga
     2821 ccgagcgcgg gatcgggttc gtgggacgtc tgctgctgca ttgcctgctc tcctcgactg
     2881 tgaacgcgcc atgaagccgg agaatagcgg acacggagtg tcggggtcaa ccggacggcg
     2941 ggtcgatgtg gggcgtcgta tcgtgcgccg gtgcggcccg taccgcggac ggcgcgagcg
     3001 gtcacggcgc ggaacccggc ccggctcatc ggtcgcgcac cgtcccttta cccgcagccc
     3061 ggatctccat ggcccgattc cgactcgaca accgcctggt cctggtcacc ggcgccgccc
     3121 gcggcatggg cctcgggctc gcccacgagt tcgcggcccg cggagcgcgc ctcgtgctct
     3181 gggacgtcga cggccccgcc ctcgttggcg ccgcggccgg cctggagcgc gaaggcctcc
     3241 gcgtgcgcac cgacacggtg gacctcctgg accgcgacgc cgtctccgcg gccgccctgc
     3301 gcgtgcttac ggaggacggc ccggtggatg tcctcgtgaa caacgccggc gtcgtgacgg
     3361 gccggccgct gctcgagctc tcggacagcg agatcgagcg caccttcggc gtgaacacgc
     3421 tggccctctt ctggacgacg cgggccttcc ttcccgccat gatcgagcgg ggcgcgggac
     3481 acgtggtgac ggtcgcatcg gccggcggcc tcgtgggcat cccgcgtctc gtggactact
     3541 gcgcctcgaa gttcgcggcc gtcggcttcg acgaggccct gcgggtcgag ctctcgacgt
     3601 cggggcactg catcggcacc accgtggtct gcccgatcta cgtggacacc gggatgttcg
     3661 agggggcgcg gacccgggtg ccttggctcc tgccggtgct ccggccggag gtcgtggtgc
     3721 ggcggacggt ccgggccgtg gagcgcggcc ggagtcgcgt gatcattccg cgcttcgcct
     3781 actctgcctt cccgctccgc ctgctgcccg tggcgtggtt cgaccggatc atggcctgga
     3841 tggggctcac gcgatcgatg gaggggtttc gggggagggg gtgaggttcg tgtcgcggcg
     3901 cgggtgcgtt tggaagcggt gtcacttcgt ttgcgggatc cggctggagg tgtggtgcat
     3961 tgctgttcgg gtgcgtttgg aagcggtgtc acttcgtttg cggcgtaaac gatcttgcat
     4021 acgcttccac agggaccagt cctgaagggt ctgcatgacc cgcaacaggc caggcggacc
     4081 cggcgccagc tccctgccgg cgcatgcagc gcggccctgg gtgcccggcg ttgcaccgtg
     4141 agggtagccg gcctccaaac cggggttgcg agggtccgga cgcctcggcc atcgtcagcc
     4201 cttccgtcag agtccgcatc ccgccgtgcc cacgttcatc ccgcgcatat tggaggccca
     4261 tctccggagc ctggccagcc gtcacccgat agtgggcatc gtcgggccac gaggcgtggg
     4321 caagaccgtc atggcgcaca gggtattttc gcgcctggat tttgtccgat gcgattcgcc
     4381 ggaagtctcc agggccctga tggaggaccc ggccggattc atccgcggat tccga
//
LOCUS       DBNHQZ010000151         6094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_994990, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000151 DBNHQZ010000000
VERSION     DBNHQZ010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_994990"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="AC16YU_03925"
     CDS             complement(<1..492)
                     /locus_tag="AC16YU_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR6064403.1"
                     /translation="MGGARIRARAIPLLKGAIWIGAFVPIGLLAYELFGGTVVVDDPV
                     ELIQRRTGLSALILLFLTLSVTPIRRITGWNRLTRVRRLLGLFAFFHATLHAFSYFVF
                     DQELSPAAIAADIVEHPWVLVGFTAFVLLIPLAVTSTNGWIRRLGGRRWARLHMLVYL
                     VPVL"
     gene            complement(503..1399)
                     /gene="msrP"
                     /locus_tag="AC16YU_03930"
     CDS             complement(503..1399)
                     /gene="msrP"
                     /locus_tag="AC16YU_03930"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR6064404.1"
                     /translation="MIRRASDIASSEITDPRLFMNRRRFMKLAAATPVAASTLHPPGG
                     PVDDDDLTPWETVTGYNNYYEFGTGKEDPKRNAQGLRTSPWSVQVAGEVEEPRTWDLG
                     ELVDGFEVQERVYRHRCVEAWSMVVPWNGFPLRDLVARLRPTPKAKYVEFTTLEDPKQ
                     MPGQRSGVLEWPYVEALRLDEAMHPLAIMVTGVYGVSLPPQNGAPIRLIVPWKYGFKG
                     GKSIVRIRFTEKQPRTTWNVAAPSEYGFYANVNPQVDHPRWSQARERRLGEFFKRDTL
                     MFNGYADEVESLYAGMDLRRFY"
     gene            1567..2052
                     /locus_tag="AC16YU_03935"
     CDS             1567..2052
                     /locus_tag="AC16YU_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR6064405.1"
                     /translation="MPRTVKRYENRKLYDPESSSYISLSRIADLVRAGETVVVLDNVT
                     GEDLTVQTLTQVILEEGRKGRALLSSDLLHDLLQRGGEAIETGMSTLRHGVDDLVQTS
                     MRRVQSFLQRPEEGELRDLREQLYRLEEMLHRMFDESEPPPGDGPAPAGPPGGQTETS
                     S"
     gene            2049..2735
                     /locus_tag="AC16YU_03940"
     CDS             2049..2735
                     /locus_tag="AC16YU_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR6064406.1"
                     /translation="MKKESEGFEWRRVPGMVADGTRELLKAGLGVVDVLEHQGERLFD
                     ALSGDRAALVEDLQDRGTRAIDEMQAEANRVFRDLVERGERLQEEGGKRIGEAVKGLS
                     DRPKEMAEAVEHTVERAVGATLRRLDVPTRHEVERLTRRVERLTEQVERLASRLAGGE
                     VPAETILEVVAVGETWCLRDLATGEILFEAETKAEAVKNGRERAHAAAPSELRILKQD
                     GTVQDTSRYA"
     gene            2728..3942
                     /locus_tag="AC16YU_03945"
     CDS             2728..3942
                     /locus_tag="AC16YU_03945"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6064407.1"
                     /translation="MPDRPSGGGGASSGVGLACAGGVVEGAFYEIGALCALEEAVDGL
                     DLNRLSAYVGVSSGAIVATFLAAGVPIHELSRAVAGYARPPLDFRPQALFRPDWTEFG
                     RRLGKLPSSVASSVRRFLRDPGDMGLIGAALGLGALIPVGLFDNAPLERFVADVLADR
                     GVANRFGAFRPALRIVATDLDSADAVCFGTPGWDGVPVSRAVQASTALPVLYRPVEID
                     GRHYIDGVARRTVHASRALETGAGLVFCVNPIVPVDLRAAPPDARHAGSDLVDAGLPL
                     VLSQTFRMLVHSRMQTGFRGYRHLFPDANTILLEPDMADRTLFFSNVFSFANRQAICM
                     KAYEATRSWLRDHADEVDPILDGHGMRLDRRILADRGLTLQGRPCDSPTAAAADRAMD
                     RLEEVLGALETD"
     gene            complement(3969..5510)
                     /locus_tag="AC16YU_03950"
     CDS             complement(3969..5510)
                     /locus_tag="AC16YU_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HIR6064408.1"
                     /translation="MFIPRLLLRKLYTVGSLSSEGGDTSFELRNRLSDATLTAFSRVA
                     IDGDEVPLDRVTVGPAGETARPAATVGPDDPLAFPLRATLAVTLGGISTGAGRHRLEI
                     AARSEPFGELAFEVEDSIASRADGGERIPRDPVDDYTAEAAARRREFAESRTGVALTH
                     TGRFDFDPHVTQGNIEHFVGVAQVPVGLAGPIRIRGEHADGEFLVPMATTEGTLVASY
                     NRGMKVLNMAGGVTCTVVGDAMQRAPVFVFDDARRGRDFVGWIETHAAEITREAESTS
                     HVARLLHVDPYQSNRFVFLRFNFSTGDAAGQNMVGRATFAACSWILEHFEGIRRFFLE
                     SNLATDKKASQVNIMRTRGKRVTAEATLPRDLLVEQLRVEPRDLAYHGQVANIGSILS
                     GANNNGLHSANAITALYIATGQDVANVAEASAGILYSELTDDGGLYVSLTIPSLIVAT
                     YGGGTGLPTQREALEMLGCYGTGKVLRFAEIVAGVALAGELSLAAAISSLEWVSSHEQ
                     YGRNR"
     gene            5613..6050
                     /locus_tag="AC16YU_03955"
     CDS             5613..6050
                     /locus_tag="AC16YU_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064409.1"
                     /translation="MTDLPRYWTPAFIEAFVERLNEDPGFQRHARRFSNAIVLQCLDT
                     PDGRDVRATYDIDAGYVSVAVVDEEAPSAVVRNGRFDSRSALARATAPYDVWRRVDDG
                     SLSILGAMASPEYRIEGSRMKIMLHLSIFDALGQVASAMPKRY"
BASE COUNT          931 a         2048 c         2218 g          897 t
ORIGIN      
        1 gagcaccggg accaggtaga ccagcatgtg cagccgggcc cagcgccgcc cgccgagcct
       61 gcggatccag ccgttcgtgc tcgtcacggc gagcgggatg aggaggacga acgccgtgaa
      121 ccccaccagc acccaggggt gctcgacgat gtccgccgcg atcgcggcgg gggagagctc
      181 ctggtcgaac acgaagtagc tgaaggcgtg cagggtcgca tggaagaagg cgaagagccc
      241 gaggaggcgc cggacgcgag tgagccggtt ccacccggtg atccgccgga tcggcgttac
      301 ggacagcgtc aggaagagca ggatgagcgc cgacagcccc gtcctccgct ggatgagctc
      361 gacgggatcg tcgaccacga ccgttccgcc gaagagctcg tacgcgagga gcccgatcgg
      421 tacgaatgcg ccgatccaga tggcgccctt gagcagcggt atggctcgtg cccggatgcg
      481 cgcgcctccc atggccgacc gcctagtaga agcgacggag gtccatcccc gcgtagagcg
      541 actcgacctc gtcggcatac ccgttgaaca tcagggtgtc gcgcttgaag aactcgccga
      601 gccgccgctc cctggcctgg ctccagcgcg ggtggtccac ctgcgggttg acgttggcgt
      661 agaagccgta ctcgctcggc gccgcgacat tccacgtggt ccggggctgc ttctccgtga
      721 accggatccg gacgatggac ttgcctccct tgaatccgta cttccagggc acgatgaggc
      781 ggatgggcgc cccgttctgc gggggaaggc tcacgccata cacgccggtg accatgatcg
      841 cgagcgggtg catggcctcg tccaggcgca gcgcctcgac gtagggccat tccaggacgc
      901 cgctccgctg tccgggcatc tgcttcgggt cctcgagcgt cgtgaactcg acgtacttcg
      961 ccttcggcgt gggcctgagc cgcgcgacca ggtcgcggag gggaaagccg ttccacggca
     1021 cgaccatgct ccaggcctcg acgcaccggt gccggtacac gcgctcctgg acctcgaagc
     1081 cgtcgacgag ctccccgagg tcccacgtcc ggggctcttc cacctcgccc gcgacctgga
     1141 ccgaccacgg cgacgtccgg aggccctgcg cgttgcgctt cgggtcctcc ttgccggtgc
     1201 cgaactcgta gtagttgttg tagcccgtca cggtctccca cggcgtgagg tcgtcgtcgt
     1261 ccacagggcc gccgggtgga tggagggtag aggcggcgac gggcgtcgcc gcggcgagct
     1321 tcatgaagcg ccgccggttc atgaagaggc gcgggtccgt gatttcggac gaagcgatgt
     1381 ccgacgcgcg gcgaatcagc atggtcggtc gtgggatgga cggtggggtc ccgtgatccc
     1441 catagacgtg aatggcgcac acatgattcc gaacccggca aaggggcgga atttcggccc
     1501 ttcccggggc acttgacaag gaatgacgca gtgcgttatc ttggcgcgtc aaaccggtcg
     1561 agaccgatgc ccaggacggt gaagcggtac gagaaccgca agctctacga tccggagtcc
     1621 agctcctaca tctcgctctc gcgcatcgcg gacctggtcc gggcgggcga gacggtggtg
     1681 gtgctcgaca acgtgacggg cgaggatctc acggtgcaga cgctcacgca ggtcatcctg
     1741 gaggaaggcc gaaagggtcg ggccctgctc tcgtcggacc tgctgcacga cctgctgcag
     1801 cgtggaggag aggcgatcga aaccggtatg tcgacgctcc ggcacggagt ggacgacctg
     1861 gtccagacct ccatgcgccg cgtgcagtcg ttcctgcagc ggcccgagga gggcgagttg
     1921 cgcgacctgc gcgagcagct gtaccggctc gaggagatgc tgcatcggat gttcgacgaa
     1981 agcgaacctc cgcccgggga cggaccggct ccggctggac cgccgggcgg ccaaaccgag
     2041 acatcgtcat gaagaaagag agcgagggat tcgaatggcg ccgcgtgccc ggcatggtgg
     2101 ccgacggaac gcgcgagctg ctgaaggccg gcctcggggt cgtggacgtc ctggagcacc
     2161 agggcgagcg actctttgac gcgctgagcg gcgatcgggc cgcgctggtc gaggacctgc
     2221 aggaccgcgg aacccgggcc atcgacgaga tgcaggccga ggcgaaccgc gtattccggg
     2281 acctcgtgga gcgcggggag cggctccagg aggaaggcgg aaagcgcatc ggcgaggccg
     2341 tgaaggggct ttccgatcgc ccgaaggaga tggccgaggc cgtggagcac acggtcgaac
     2401 gggccgtcgg ggcgacgctt cgccgcctgg acgtgccgac gcggcatgaa gtggaacgcc
     2461 tgacgcgtcg ggtggagcgc ctgaccgagc aggtggagcg cctggcctcc cggcttgccg
     2521 gcggcgaggt cccggccgag acgatcctcg aggtcgtggc cgtgggagag acctggtgcc
     2581 tgcgcgacct cgcgacgggc gagatcctgt tcgaggccga gaccaaggcg gaggcggtga
     2641 agaacgggcg cgagagggcg catgccgccg cgccgtccga gctccgcatc ctgaagcagg
     2701 acggaacggt gcaggacacc tcgcgctatg cctgatcgac cctccggcgg gggcggggcg
     2761 agctccggcg tgggcctggc ctgcgccggc ggcgtggtcg agggggcctt ctacgagatc
     2821 ggtgccctgt gtgccctcga ggaggccgtc gacggcctgg acctgaaccg gctgtcggcc
     2881 tatgtcggcg tgagctccgg ggccatcgtc gcgacctttc tcgcggccgg cgtaccgatc
     2941 cacgagcttt cgcgggcggt cgccgggtac gcccggccgc cgctcgattt ccgtccccag
     3001 gcgctgttcc ggccggactg gacggaattc gggcggcgcc tggggaagct gccgtcgtcg
     3061 gtcgcgagct cggtgcggcg tttcctgcgc gacccgggcg acatgggcct gatcggggcg
     3121 gcgctcgggc tcggcgcgct gatcccggtc ggccttttcg acaacgcgcc gctggagcgc
     3181 ttcgtcgccg acgtgctggc ggaccgcggc gtggccaacc gcttcggtgc gttccgccct
     3241 gccctccgga tcgtggccac ggacctcgat tcggcggacg ccgtgtgctt cggcacaccc
     3301 gggtgggacg gcgttccggt gtcccgggcc gtgcaggcga gcacggccct gcccgtgctc
     3361 taccggccgg tggagatcga cgggcggcac tacatcgacg gcgtcgcgcg gcggaccgtg
     3421 cacgccagcc gggcgctcga gaccggcgcg ggcctcgtct tctgcgtgaa cccgatcgtg
     3481 ccggtggacc ttagggccgc gccgcccgac gcccggcacg ccggatcgga cctggtggac
     3541 gccgggctcc cgctggtcct ctcgcagacc ttccggatgc tggtccactc gcggatgcag
     3601 accggattcc gcggttaccg gcacctgttt ccggatgcga acaccatcct gctcgagccg
     3661 gacatggccg accggacgct cttcttctcg aacgtcttca gcttcgccaa ccggcaggcg
     3721 atctgcatga aggcctacga ggccaccagg tcctggctgc gggaccacgc cgacgaggtg
     3781 gatccgatac tcgacggaca cggcatgcgc ctggaccgga ggattctcgc cgaccggggc
     3841 ctcacgctgc agggaaggcc gtgcgacagt ccgactgcgg cggcggccga ccgggccatg
     3901 gaccggctcg aggaggtgct cggcgcgctg gaaacggact gacccgcggg ccgaacggcc
     3961 acagcggttc agcggttgcg gccgtactgt tcgtggctgg acacccactc gagcgacgag
     4021 atcgccgcgg ccagcgagag ctcgcccgcg agcgcgacgc cggccacgat ctcggcgaag
     4081 cgcagcacct tgccggtccc gtagcagccg agcatctcga gcgcctcgcg ctgggtcggc
     4141 agcccggttc cacccccgta cgtcgcgacg atgaggctcg ggatggtgag cgacacgtac
     4201 aggcccccgt cgtcggtcag ctctgagtac aggatgccgg ccgacgcctc cgccacgttc
     4261 gcgacgtcct gcccggtcgc gatgtagagt gccgtgatcg cgttcgccga gtgcagcccg
     4321 ttgttgttgg ccccggagag gatcgacccg atgttggcca cctgcccgtg gtaggcaagg
     4381 tcgcgcggct ccacgcggag ctgctcgacc aggaggtcgc gcgggagggt ggcctcggcg
     4441 gtcacccgct tgccgcgcgt ccgcatgatg ttgacctgcg acgccttctt gtcggtcgcc
     4501 aggttcgatt ccaggaagaa gcgccggatg ccctcgaaat gctccaggat ccacgagcac
     4561 gcggcgaagg tggcgcggcc gaccatgttc tgcccggcgg cgtcgcccgt cgagaagttg
     4621 aagcgcagga agacgaaacg gttcgactgg tacggatcca cgtggagcag ccgggccacg
     4681 tgcgaggtgg actcggcctc gcgggtgatc tccgcggcgt gcgtctcgat ccacccgacg
     4741 aagtcccggc cgcggcgcgc gtcgtcgaac acgaagacgg gcgcccgctg catcgcgtcg
     4801 cccacgaccg tgcaggtgac gccgccggcc atgttcagga ccttcatgcc ccggttgtac
     4861 gaggcgacga gcgtgccctc ggtggtcgcc atcgggacca ggaactcgcc gtctgcatgc
     4921 tccccccgga tccggatcgg gccggcgagg ccgacgggga cctgcgcgac ccccacgaag
     4981 tgctcgatgt tgccctgtgt gacgtgggga tcgaaatcga accgccccgt gtgcgtgagc
     5041 gcgacgcccg tcctggactc ggcgaactcg cggcggcgcg cggccgcctc cgccgtgtaa
     5101 tcgtccacgg gatcgcgggg gatgcgctcg ccgccgtcgg cccgggaggc gatgctgtcc
     5161 tccacctcga acgcgagctc gccgaagggc tccgatcggg cggcgatctc aaggcgatgg
     5221 cgcccggcac ccgtcgagat gccgccgagc gtcacggcca gcgtggcgcg gagcggaaag
     5281 gcgaggggat cgtccggacc gaccgtggcg gccggccggg cggtctcgcc ggccgggccg
     5341 acggtgaccc ggtcgagcgg gacctcgtcg ccgtcgatcg cgacacgcga gaaggccgtc
     5401 agggtggcgt cggagagccg attgcgcagc tcgaacgagg tgtcgccgcc ctcggaggac
     5461 aggctgccga ccgtgtacag cttgcgaagc aggaggcgcg gaatgaacat ggcgtcggat
     5521 ccaggaatcc agccggcacc gttcgtcgtt cgggctcgaa gccggccgcc ggcggcgccg
     5581 gatcgaggag aatagccttc cacaggatag ccatgacgga cctgccccgc tactggacac
     5641 cggccttcat cgaggccttc gtggagcgcc tgaacgagga tcccggtttc cagcgccacg
     5701 cgcgccgctt ctcgaatgcg atcgtgctgc agtgcctgga cacgccggac gggcgcgatg
     5761 tccgcgcgac ctacgacatc gacgcggggt acgtgtccgt cgccgtcgtg gacgaggagg
     5821 caccgagtgc cgtcgtccgg aacggccggt tcgactcgcg ctcggccctc gcgcgggcca
     5881 cggccccgta cgacgtctgg cgccgggtcg acgacggctc cctgagcatc ctgggcgcca
     5941 tggcctcgcc cgaataccgc atcgagggct cgcgcatgaa gatcatgctg cacctgtcga
     6001 tcttcgacgc cctggggcag gtggcctcgg cgatgcccaa gcggtactga gaccccggcc
     6061 gtcgcgccct gcccacggcg gctagcgggc ggcc
//
LOCUS       DBNHQZ010000152         3160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1671521, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000152 DBNHQZ010000000
VERSION     DBNHQZ010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1671521"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1993
                     /locus_tag="AC16YU_03960"
     CDS             <1..1993
                     /locus_tag="AC16YU_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064410.1"
                     /translation="DANRDLFYRASADGVTWEPARMLLDNPNPDRYEMYVQAVGSAGG
                     SIVVAAVVQLDWETWTQSMLVLRSTDGGTTWSSPVSVGTPGFEMYPSAIDREPDGTLW
                     MAATGVRQWDGPSDNPFENTADVHYFTSEDDGLTWDGPVVFTSYLGDDHSPDIAMSPD
                     GPLVVFQSARVSPQGIALGVLGLTPDSNSPPVVGLWVLPYEGLAAGASTEIVTAAGSA
                     VGVASAGLVLTVDGAESEISMAQGSDLCAAYNLGYDFCWVGTIGPFDLGSEVMVRSVA
                     TDNNGRSATSWTLAIAVLAVHNVGSVTLPVQPNGFISNVMWGPSGDDYMCCGGMWAGT
                     SAGGGTVVQEYNWIATDGVTPTMGPGESDLDIAVSYHDGGLLGLGVVQRSYQWADGQR
                     SNGIVIEYEYTNDGSGGDIDALFAAMSLDADLTNGNLFWDDLIAYSADANLIYMYDSG
                     EQCATMGPCPEGYIGIAVIGDASGSPGVTLVPHTATTNSFGDTSWNLMRSGINPELTS
                     PTPVAPGDHRMLLTAQPFALGSGASQKVAFALVMGTSPEDLAASAQALALAYDTHVTG
                     VEHVVGGEIPGEFQLEQNYPNPFNPATTLRFALPRASKATLTVHNVLGQQVATLVSGD
                     LAAGVHDVRWDAEGMPSGLYLYRLEAGDYTATRRMVLVK"
     gene            complement(2090..2395)
                     /locus_tag="AC16YU_03965"
     CDS             complement(2090..2395)
                     /locus_tag="AC16YU_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064411.1"
                     /translation="MKRLFSAAVLAAVAITVFTALAPTLTRAEPAPDPCWGQATAVFA
                     QLGEMGQHASQEPTPRLGLRNLARALYDAGVIPDDSMAALGAFVADALGLSIDACTA"
     gene            complement(2763..>3160)
                     /locus_tag="AC16YU_03970"
     CDS             complement(2763..>3160)
                     /locus_tag="AC16YU_03970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6064412.1"
                     /translation="HEIAHAVARHSNERMSQALLVQLGGQTLSAALQQKPQQTQDIWM
                     SLFGVGTQVGAVLPHNRLQETEADHLGLIFMAMAGYDPNGALEFWQRMSADGGASSPE
                     FLSTHPSDSNRIEKIRSEIPEAMRYYTRQ"
BASE COUNT          515 a         1033 c         1113 g          499 t
ORIGIN      
        1 ggacgccaat cgcgacctct tctaccgcgc gagcgccgac ggggtcacgt gggagccggc
       61 caggatgctc ctggacaatc cgaacccgga tcgctacgag atgtacgtcc aggccgtggg
      121 gtcggccggc ggcagcatcg tggtcgcggc cgttgtccag ttggactggg aaacgtggac
      181 gcagagcatg ctcgttctgc gcagcacgga cggcggaacg acctggtcga gcccggtgag
      241 cgtcgggacg ccgggcttcg agatgtaccc gtcggccatc gaccgtgagc cggacgggac
      301 gctgtggatg gcggccacgg gggttcggca gtgggacggt ccaagcgaca atccgttcga
      361 gaacaccgcg gacgtccact acttcacgag cgaggacgac gggctgacgt gggacgggcc
      421 ggtcgtcttc acgtcgtacc tgggcgacga ccactcgcca gacatcgcga tgtcaccgga
      481 cgggcccctg gtcgtcttcc agtccgctcg cgtatccccc cagggaatcg ccctcggtgt
      541 cctcgggctg actccggact ccaactcccc gcccgtcgtc ggcctctggg tgcttccgta
      601 cgaaggactg gccgcaggcg cgtcgaccga gatcgtgacg gctgcaggat cggctgtggg
      661 cgttgcctcc gccggcctgg tcctgacggt ggacggggcg gagagcgaga tctccatggc
      721 tcagggctcg gacctgtgcg ccgcatacaa cctgggctac gacttctgct gggtcggcac
      781 catcggaccg ttcgacctgg gctcggaggt catggtccgg tcggtcgcca cggacaacaa
      841 cggccgatcg gcgacctcct ggacccttgc gatcgccgtc ctcgcggtgc acaacgtggg
      901 cagcgtgacc ctgccggtcc agcccaacgg gttcatcagc aacgtgatgt ggggcccctc
      961 cggagacgac tacatgtgct gtggcggcat gtgggcggga acgtccgctg gcggcgggac
     1021 ggtggtgcag gagtacaact ggatcgcgac ggacggcgtc acaccgacca tgggacccgg
     1081 cgagtcggac ctggacatcg ccgtctcgta ccacgacggc gggctgctgg gactcggcgt
     1141 cgtgcagcgc agctaccagt gggccgatgg tcagcgctcg aacggcatcg tgatcgaata
     1201 cgagtacacg aacgacggat ccgggggcga catcgacgcg ctgttcgcgg cgatgagcct
     1261 ggacgcggac ctgacgaacg gcaacctgtt ctgggacgac ctgatcgcgt actcggccga
     1321 cgcgaacctg atctacatgt acgattcggg cgaacagtgc gccactatgg gtccgtgtcc
     1381 ggaagggtac atcgggatcg cggtgatcgg tgacgcctcc ggcagtccgg gcgtcacgct
     1441 cgtgccgcac accgccacca cgaacagctt cggcgacacc tcctggaacc tgatgcggag
     1501 cgggatcaac ccggagctca cctccccgac gcccgtggct ccaggggacc accgcatgct
     1561 gctcacggcc cagcccttcg ccctcgggtc gggcgcgtcg cagaaggtgg ccttcgcgct
     1621 cgtcatggga acgagcccgg aagacctggc cgcgagcgcg caggcgctcg ccctggcgta
     1681 cgacacgcat gtgacgggcg tcgagcacgt ggtcggcggc gagattcctg gcgagttcca
     1741 gctggagcag aactacccga atccgttcaa tccggcgacg acgctccgct tcgccctgcc
     1801 ccgggcatcg aaagccacgc tcaccgtgca caacgtgctc gggcagcagg tggcgacgct
     1861 cgtctcgggc gatctcgcgg ccggcgtgca cgacgtgcgc tgggatgccg aggggatgcc
     1921 gtcgggcctg tacctgtacc ggctcgaggc cggcgactac acggcaacga ggcggatggt
     1981 actcgtcaag tgagataatc cggatcgagc ccccgcggtc cggataacgg aagcccctgc
     2041 cgaccccatg gccggcaggg gcttctcgct ttcgcgaaac ctgccgtccc taggccgtgc
     2101 aggcatcgat cgagagcccg agcgcatccg cgacgaaggc cccgagagcg gccatcgagt
     2161 cgtcaggaat cacgccggcg tcgtagagag cacgagccag gttgcgcagg cccagccgcg
     2221 gcgtcggttc ctggctggcg tgctgaccca tttcgcccaa ctgggcgaac accgccgtcg
     2281 cctggcccca gcaggggtcc ggcgcgggct cggcgcgggt cagggtcggg gcaagcgcgg
     2341 tgaaaacggt gatcgcgacc gcggccagga cggccgccga aaacagcctc ttcatgaggg
     2401 cacctccttg gactgggtga ctgggggtgt cacaagggta gccgaaaccg tggattattg
     2461 aaagtgcttc cgcctcccgt actcgacggg tactcggacg ggggcggaca ccgggcgtgg
     2521 agaacggcat ccccatcctg cgggtcggcc gcggcgacgg tcgccggccg cgcatccagg
     2581 gccggacccc taatggcacg agcccctgcg gacttacggg tccgcagggg ctgcgccgga
     2641 ttgaacggtt cgcaagagac cggggctgga acaggcatgg ccagcttgcc gattcgccag
     2701 ccccgcaggc gtgcgccgga ttggggggga ggggaatccg gtcgcagacc acggaccgtc
     2761 aatcactgtc gcgtgtagta cctcatggct tcggggattt cggatcggat cttctcgatc
     2821 cggttgctgt ccgaggggtg ggtgctcagg aactccggcg agctcgcgcc cccgtctgcc
     2881 gacatgcgct gccagaactc gagggcgccg ttcgggtcgt atccggccat cgccatgaat
     2941 atgagcccga ggtgatcggc ctcggtttcc tggagacggt tgtgagggag gaccgcgccg
     3001 acctgggtgc cgacaccgaa cagggacatc cagatatcct gggtctgctg cggcttctgc
     3061 tggagcgcgg cggacagggt ctgacctccc agctgaacga gcagcgcctg gctcatccgt
     3121 tcgttgctgt gcctggccac cgcgtgggcg atctcgtgtc
//
LOCUS       DBNHQZ010000153         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2348377, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000153 DBNHQZ010000000
VERSION     DBNHQZ010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2348377"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="AC16YU_03975"
     CDS             <1..564
                     /locus_tag="AC16YU_03975"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR6064413.1"
                     /translation="VVDELMANGRGPYGEPPDTHPVRSGRLRGVLELAAEKAGWGQPL
                     PAGQGMGIAAHYSFWGYAAFVVRVAVDGSRVRVLRVDAAIDCGTVVNPDRVRAQQEGA
                     VVFSLTHALHSEIIAREGAVVTGNFDEYPLLELRETPETHVHLVESTEPSGGVGEPGI
                     PPMMPALTNAILAATGTPVRELPIRLA"
     gene            complement(577..1620)
                     /locus_tag="AC16YU_03980"
     CDS             complement(577..1620)
                     /locus_tag="AC16YU_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6064414.1"
                     /translation="MRDVLPFLADAIARRRPVGWARVVSTWGSAPRRPGACMAVVSPV
                     EVAGSVSGGCVEHAVIDEVLGSLASGRTQVVEYGIRDDEAWSAGLSCGGTVRILVEAF
                     PLDYDENGACGVGCTLADAVRTGRPAVLVTPLDDSGGPALFDAEGLLLDGEEPPDGVA
                     EAALQALAAGAPREMDLACGRWFVHPVPSRERLFIVGGADITVHLVPLARRVGFETIV
                     LDPHGVFSDGRTFEEAPDRIVHGPAETGLPGIDPRTYVVLLTHDPSVDDPVLHRLLGS
                     DVAYVGALGGRKTQDRRNERLREAGFPDEAIARIDGPVGVPIGAVTPAEIAVSIVARL
                     VETRGRRLRPARP"
     gene            complement(1671..2255)
                     /locus_tag="AC16YU_03985"
     CDS             complement(1671..2255)
                     /locus_tag="AC16YU_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6064415.1"
                     /translation="MSEPRITVILLAAGRSSRFGPADKLTADLQGRPVIRRAAEAALA
                     VGSVDVCVVTDSEGVGEALRALPVRFARADPIDGAMGSSIAAGVRASPDAAAWLVWPG
                     DMPLIRPETARRVVATYHSDWPVVPRSDAGRGHPVLFPARFRPQLEALREDRGARPIL
                     LAADRVLEVHVDDPGIHLDIDSPEDLRTAARLVR"
     gene            2419..>2731
                     /locus_tag="AC16YU_03990"
     CDS             2419..>2731
                     /locus_tag="AC16YU_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064416.1"
                     /translation="MERTSVVVRRISHGRDLRTFIRLPFEANAANTKWAAPLNSDEWH
                     RLDTNRNPAMAYCDAAPALAERNGRAVGRIMGIVNRRHNEQSGEAVGRFGFFDCEDDP
                     ET"
BASE COUNT          418 a          942 c         1011 g          360 t
ORIGIN      
        1 gtcgtggacg agctcatggc gaacgggcgc gggccgtacg gggagccgcc cgatacgcat
       61 cccgtgcgct ccggcaggct gcggggcgtc ctggaactgg ccgcggagaa ggccgggtgg
      121 gggcagccgc tgcccgcggg tcagggcatg ggcatcgcgg cgcactactc gttctggggc
      181 tacgccgcct tcgtcgtgcg cgtggcggtg gacgggagcc gggtgcgtgt gctccgcgtg
      241 gacgccgcca tcgactgcgg gaccgtcgtg aatccggacc gcgtccgggc gcagcaggag
      301 ggggccgtcg tattcagcct gacccacgcg ctgcactccg agatcatcgc gagggaaggc
      361 gccgtcgtga cgggcaattt cgacgagtat ccgctcctgg agctgcgcga gacgcccgag
      421 acgcacgtgc acctggtgga gtcgaccgaa ccctcgggcg gcgtcggcga gccggggatt
      481 ccgccgatga tgcccgcact caccaacgcg atcctcgcgg ccacggggac tcccgtgcgc
      541 gagctgccga tccgactggc ctgagccctc tttccgctac ggccgcgccg gccgtagtcg
      601 tcggccgcgc gtctcgacca ggcgggcgac gatgctcacg gcgatctcgg ccggcgtgac
      661 ggccccgatg ggcacgccca cggggccgtc gatgcgggcg atggcctcgt ctgggaaccc
      721 ggcctcgcgc aggcgctcgt tgcggcggtc ctgcgtcttt cggcccccga gggcgccgac
      781 gtaggccacg tccgaaccca ggagccggtg caagacgggg tcgtcgaccg acggatcgtg
      841 ggtgaggagg acgacgtacg ttctggggtc gatccccggc agcccggtct cggccggacc
      901 gtggacgatc cggtcgggcg cctcttcaaa ggtccgtccg tccgaaaaca ccccgtgggg
      961 atccaggacg atggtctcga acccgacccg ccgggcgagt ggcaccaggt gcacggtgat
     1021 gtcggcgccg cccacgatga agagccgctc ccgggaaggg accgggtgga cgaaccaccg
     1081 gccgcacgcg aggtccattt cgcgtggggc gcccgcggcc agcgcctgga gggccgcctc
     1141 ggcgaccccg tcggggggtt cttcgccgtc gagcaggagc ccctcggcgt cgaacagcgc
     1201 ggggcctccg gagtcgtcga gcggcgtgac gagcacggcc gggcggcccg tccggacggc
     1261 atccgcgagc gtgcagccca cgccgcatgc tccgttctcg tcgtaatcga ggggaaacgc
     1321 ctcgacgagt atgcggacgg taccaccgca gctcagcccc gccgaccagg cctcgtcgtc
     1381 gcggatcccg tactccacga cctgcgtgcg gccggacgca agcgatccga gcacctcgtc
     1441 gatgacggcg tgctccacgc atcccccgct cacggatccg gcgacttcga cgggcgagac
     1501 caccgccatg caggccccgg gcctccgtgg agccgaaccc caggtcgaga ccacccgggc
     1561 ccagccgacc ggccggcggc gtgcgatggc atcggcaaga aagggaagga cgtcgcgcat
     1621 cgtaaatgga ccacggatcg cgtgaaagta ccgggcgatc ccgtggcggg tcaacgtacc
     1681 aggcgcgccg ccgtccgaag atcctcggga gaatcgatgt ccaggtggat ccccgggtcg
     1741 tccacgtgca cctccagcac gcggtcggcg gcgagcagga tcgggcgcgc tccccggtcc
     1801 tcacgcagcg cctcgagctg cggacggaac cgggccggga agagcaccgg gtggccccgt
     1861 cccgcgtccg aacggggtac cacgggccag tcggagtgat aggtggcgac gacccgccgg
     1921 gcggtttcgg gtcgtatcag cggcatgtcc ccgggccaga cgagccaggc cgcggcgtcg
     1981 ggggatgctc ggacaccggc cgctatggac gatcccatgg cgccatcgat cggatcggcc
     2041 cgggcgaagc ggaccggaag ggcgcgaagg gcctctccga ccccctcgct gtccgtcacg
     2101 acgcacacgt cgaccgatcc gacggcgagc gcggcctcgg cggcgcgccg gatcacgggg
     2161 cgcccctgca ggtcggccgt cagcttgtcg gcggggccga accggctcga gcggccggcc
     2221 gcgagcagga tgacggtgat gcggggctcc gacatgtggc gaagatactg tcggccggtg
     2281 ggcccggccc ggccgaagcc accggccgcg gacttgacgg ccgtgttccg aaccgtacat
     2341 tccatccttc gagcgccggg tcgagcgaac tgccgccggg cgcgtggttg tgggtcgagc
     2401 agtcggaaaa ccggagtcat ggagcgcaca tcagtcgtgg tacgccgcat ctctcacggg
     2461 cgggatcttc ggacgttcat ccggctgccc tttgaagcca acgcggccaa cacgaaatgg
     2521 gccgcgccgc tgaactcgga tgagtggcat cgactcgaca cgaacaggaa tccggcgatg
     2581 gcatactgcg acgccgctcc cgcgctcgcg gagcggaacg ggcgggccgt gggtcgcatc
     2641 atgggcatcg tgaaccggcg gcacaacgag cagtccggag aggcggtagg acggttcggc
     2701 ttcttcgact gcgaagacga tccggagacg g
//
LOCUS       DBNHQZ010000154         5699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1942957, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000154 DBNHQZ010000000
VERSION     DBNHQZ010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1942957"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..2399
                     /locus_tag="AC16YU_03995"
     CDS             165..2399
                     /locus_tag="AC16YU_03995"
                     /EC_number="3.4.14.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR6064417.1"
                     /translation="MRAAIVALLVLLSACAPPRQVTRVTDRPVPPATGSPEAETSPAT
                     PIAPGAASTVKAGRFDGGKMWTFDDPPLAWFEEAYGLRPDSSWFARARGAALRFADYC
                     SASFVSPYGLVMTNHHCGRESVSKVSGGEEALLDNGFYAASLAEERRVKDLFVEQLVG
                     IRDITSDVYSATAAARTNMERAEARRNRAEALEKRLTSEAKATDSTLSVQVIALYDGG
                     RYAAYTFRRYDDVRLVQAPELQIGFFGGDWDNFTYPRYNLDMSFFRVYGKDGKPVRPE
                     HWFPVDPTGADEGEAVFVVGNPGSTSRLATVSQLEYDRDIELPLVLQVLEKRSAILSG
                     FIESSPDTAEAYDLRNTWFSLQNSIKSTRGQLEGLRSDWLIPRRAAAEDVLQRAILSV
                     DSLRGQYGSVFDDLRAIDGSKRASAGQAAAFTAFGSPDVSSHVLLRALYGYVVTLLGQ
                     RGAPESQLEEIREEAREIEDRPDEIERAFLAARLEELRGALGENDPTVKRLFAGRSAD
                     SIAVAVIARTALTDSAGFAALLDGSYLSSGDATVPLIEAVAPLYFTLSQQLQNFEQQE
                     EELNSRLARARFALHGTSIPPDASFSLRIADGIVGGYPYNGTQAPPFTTFYGMYERHH
                     ALDDHPDWRLPDRWSGPPGDLDLGTPMNLVSTNDIAGGNSGSPLLDSELRVVGLVFDS
                     NIEALPNEYLYTDERGRSISVDIRGILESLDVLYDADRIVSELVDHELHETEAEADEY
                     RASR"
     gene            complement(2396..3331)
                     /locus_tag="AC16YU_04000"
     CDS             complement(2396..3331)
                     /locus_tag="AC16YU_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6064418.1"
                     /translation="MSSAPGVNEDFRPEGILFVFVDGIGQGDDDPGYNPLAVDRPGLL
                     AMSGGVRWVSGDVPPARGAVFRPIDACLGIEGYPQSGTGQASLFTGENCARLAGRHYG
                     PFPHSTSRPALRSRSIFARIGADRCAFANAFPPRYFEVMQARDRWPVMTRACLDAGVP
                     VRTLAELRAGDAIAADLTGRGLRDMGLDVEVLTPAGTAANALRLAGRHRLTVVEIFHT
                     DKAGHARDRSAADRVLDDLDAFLSALVGKRPAGLLVVLTSDHGNLEDLRVRTHTRNPV
                     PFAALGPGAGRLSGVRSLVDVTPALVGLVRGEQDA"
     gene            complement(3469..3541)
                     /locus_tag="AC16YU_04005"
     tRNA            complement(3469..3541)
                     /locus_tag="AC16YU_04005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3506..3508),aa:Met,seq:cat)
     gene            3727..4920
                     /locus_tag="AC16YU_04010"
     CDS             3727..4920
                     /locus_tag="AC16YU_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR6064419.1"
                     /translation="MAILHPFRAVRPDPARAADVACVPYDVIDTREARELANGRPLSF
                     LHVIRPEIDLPEGTDEHADAVYERGGANLRAFVDADYTLREDVPSLYLYRLVMGARAQ
                     TGIFGCVSVDDYDSDVILKHEKTRQSKEDDRTRHILTQAAHAEPVMLIFRDDPAVTAM
                     MDACAAGEPLYDLTADDGVRHTIWRFRDARPLVEAFAGIDRLYVADGHHRCKAASRAA
                     AEVEGEEVRYFPAVLFPMRMMHIMPYNRVVRRVPGGVDALVQALGERYGLERDAFPSP
                     ARAGRVSVYAKGAWLGLDLPASSRPGPTDRLDVGRLTEYVLEPLLGITDPRTDANIDF
                     VGGIRGTAELERLVDSGAAEVAFSMYPTSIGQLVEVSDAGLLMPPKSTWFEPKLRSGL
                     LVHTF"
     gene            4944..>5699
                     /locus_tag="AC16YU_04015"
     CDS             4944..>5699
                     /locus_tag="AC16YU_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6064420.1"
                     /translation="MKIFLADKLSSSCVDALERGGHEVVSRPDAKGDSLVEALRSENP
                     AVLVVRSTKVPESVLDAAPALELVVRAGAGYDTIDVDAASSRGIFVSNCPGKNSVAVA
                     ELTLGLVMALDRRIPDNVADARGGTWNKAEYSKAPGLKGRTLGLVGLGSIGREVARRA
                     EAFGMRVIGWSRSLTPESAAEMGVGFRASPLEVARDADIVSLHVAATPDTRHLADRPF
                     FEAMNPGAHFVNTTRSSVVDEEALVWAVENRGIR"
BASE COUNT          890 a         1955 c         1984 g          870 t
ORIGIN      
        1 caccagatcc taaatctggc gcgtctgcca gttccgccac tttcgcgacg gccgaaaggt
       61 actgcgacgg gtcgcgcgtc gccagcggac gcgggcgcac ggaacgcaaa cggccggtcg
      121 gcgtggacgg tggctcgcga tacaatgccc atccttccga atccatgcgt gctgccatcg
      181 tcgccctcct cgtgctgctc tcggcctgcg cgccgccccg gcaggtcacc cgggtgaccg
      241 atcgtcccgt gccgccggcg acggggagcc ccgaggccga gacatccccc gcgacgccga
      301 tcgcgcccgg agcggcatcg acggtgaagg caggccggtt cgacgggggc aagatgtgga
      361 cgttcgatga cccgccgctg gcctggttcg aggaggcgta cggcctgcgt ccggactcgt
      421 cgtggttcgc ccgggcccgc ggcgccgcgc tccggttcgc ggattactgt tcggcgtcgt
      481 tcgtctcgcc gtacggcctt gtcatgacga accaccactg cgggcgcgaa agcgtttcga
      541 aggtcagcgg gggggaagag gcgctcctgg acaacggatt ctatgcggcc tcgctcgcgg
      601 aggagcggcg cgtcaaggac ctgttcgtcg agcagctcgt gggcatccgg gacatcacct
      661 cggacgtcta ctcggcgacg gccgcggcgc gcacaaacat ggagcgggcc gaggcgcggc
      721 gcaaccgggc ggaagcgctc gagaagcgcc tcacttcgga ggccaaggcc accgactcca
      781 cgctctccgt gcaggtgatc gccctctatg acggcggacg ctacgcggcc tacacgttcc
      841 gccgatacga cgacgtccgc ctggtgcagg cgccggaact ccagatcggg ttcttcgggg
      901 gtgactggga caatttcacg tatccccgct acaacctgga catgtcgttc ttccgcgtgt
      961 acggaaagga cggcaagccc gtccggcccg agcactggtt cccggtggac ccgacgggcg
     1021 ccgacgaagg tgaggccgtc ttcgtcgtcg gcaacccggg ttccacgagc cggctcgcca
     1081 cggtgtccca gctcgagtac gaccgggaca tcgagctgcc tctcgttctg caggtcctcg
     1141 agaaacggtc ggccatcctg tccgggttca tcgagagcag cccggacacg gcggaggcct
     1201 atgacctccg caacacgtgg ttctcgctcc agaactcgat caagtccacg cggggccagc
     1261 tcgagggtct tcgctccgat tggctcatcc cgcgacgggc cgcggccgag gatgtgctgc
     1321 agagggccat cctgtcggtg gactcgctcc gcggacagta cggctcggtc ttcgacgacc
     1381 tccgggccat cgacggctcg aaacgggcgt ccgccggcca ggcagccgcg tttacggcct
     1441 tcggctcgcc cgacgtgagt tcgcatgtcc tgctgcgggc gctgtacggg tacgtggtca
     1501 cgctcctcgg gcagcgcggg gcacccgagt cgcagctcga ggaaatccgg gaggaggcgc
     1561 gcgagatcga ggaccgtccg gacgaaatcg agcgggcgtt tcttgcggcc cgcctggagg
     1621 agcttcgcgg cgctctcggg gagaacgatc cgacggtgaa gagactcttc gcgggccgga
     1681 gcgccgattc gatcgccgtc gcagtcatcg cccggacagc actcaccgat tcggccggct
     1741 tcgcggcgct gctcgacggc tcgtacctct cgagcggcga cgcgaccgtc ccgctgatcg
     1801 aggccgtcgc cccgctctat ttcacgctca gtcaacagct ccagaatttc gagcagcagg
     1861 aggaagagct gaactcgcgc ctggcccgcg cccggttcgc ccttcacggc acgtcgattc
     1921 cgccggacgc ctcgttctcc ctccggatcg cggacggtat cgtcggaggt tacccgtaca
     1981 acggaaccca ggcaccgcct ttcaccacct tttacgggat gtacgagcgc catcatgcgc
     2041 tggacgatca tccggactgg aggctgccgg atcgctggtc gggcccgccc ggggacctgg
     2101 acctcggcac cccgatgaac ctggtgtcga ccaacgacat cgccgggggc aactcgggct
     2161 cgccgctgct ggacagtgag ctgcgcgtcg tgggcctggt gttcgactcg aacatcgagg
     2221 ccctccccaa cgagtatctg tacaccgacg aacggggtcg ctcgatctcg gtcgacatcc
     2281 gcggcatcct cgaatcgctc gacgtcctct acgacgcgga ccggatcgtg agcgagctgg
     2341 tcgatcacga gctgcacgag accgaggcgg aggcggacga gtaccgggcg agtcgctagg
     2401 cgtcctgctc accgcggacg agcccgacga gcgcgggagt cacgtcgacg aggcttcgga
     2461 cgcccgacaa ccgcccggcg ccggggccga gcgccgcgaa cggcaccggg ttgcgggtgt
     2521 gcgtccgcac ccggaggtcc tcgagatttc cgtggtcgga cgtgagcacg accagcagtc
     2581 cggccggacg tttgccgacg agcgccgaga gaaacgcgtc caggtcgtcc aggacccggt
     2641 cggcggcgga ccggtcccgg gcatgccccg ccttgtccgt gtggaagatc tcgacgacgg
     2701 tcagccggtg gcggccggcc agacgcaggg cgttggccgc cgtccctgcg ggcgtcagca
     2761 cctcgacgtc gagtcccatg tcccgcagcc cccggccggt caggtcggcc gcgatggcgt
     2821 cgcccgcgcg gagctccgcg agcgtccgga ccggcacccc ggcatccagg cacgcccgcg
     2881 tcatgacggg ccagcggtcc ctggcctgca tgacctcgaa atagcgcggc gggaaggcgt
     2941 ttgcgaaggc gcaccggtcc gcgccgatcc gggcgaagat gctacgggag cgaagcgccg
     3001 gccgggacgt ggagtggggg aagggaccgt aatgccgccc tgcgagtcga gcgcagttct
     3061 ccccggtgaa cagcgaggcc tggcccgtcc cggactgggg ataaccctcg atcccgaggc
     3121 aggcgtcgat gggacggaac acggctccgc gcgccggcgg aacgtccccc gaaacccacc
     3181 gaacgccccc gctcatcgcg aggagccccg gccgatcgac ggccagcggg ttgtagcccg
     3241 ggtcgtcgtc tccctggccg ataccgtcca cgaagacgaa caggatgcct tcgggacgga
     3301 agtcctcgtt cacgccgggt gcgctgctca aggcagggtc ttctgacacg acggggaata
     3361 tcggggcgcg gtgagcgacg tgcacgactc cgctggtgga cggcgcggcc gatcgggacc
     3421 ccacaaaaaa accgagccgc ccggcattcc ggacggctcg gccttctgta gcgggggcgg
     3481 gattcgaacc cgcgacctcc gggttatgag cccggtgagc taccggctgc tccaccccgc
     3541 ggtaggctga caatatacgg ctcctggtgg aggcttccaa gcggttcccg ggcacggcac
     3601 gcccctcgcg agggcggcgt cgcggcggaa gcgctgccga atccatcgtg tctttttggt
     3661 taacgcccgg aagcgcgcgg gggtttggtt attttcgccc cgatagtcgt cgcagccatt
     3721 tcccccatgg ccattctcca tccgttccgg gccgtaaggc ctgatccggc ccgcgcggcc
     3781 gacgtggcct gcgtccccta cgacgtgatc gatacccgcg aagcgcgcga gctggcgaat
     3841 ggcaggccgc taagcttcct tcacgtcatc cggcccgaga tcgaccttcc cgagggcacg
     3901 gatgagcacg cggacgcggt gtacgagcgg ggaggggcca acctgcgtgc cttcgtcgac
     3961 gcggactaca cgctgcgcga ggatgtcccc tcgctatacc tgtatcgtct ggtcatggga
     4021 gcgagggccc agacgggcat cttcgggtgc gtttccgtgg acgactacga ctcggacgtc
     4081 atcctgaagc acgagaagac gcgacagtcc aaggaggacg atcggacccg ccacatcctc
     4141 acccaggctg cccacgcgga accggtgatg ctcatcttcc gggacgaccc ggccgtgacc
     4201 gcgatgatgg acgcttgcgc ggcgggcgag ccgctctacg acctgacggc ggacgacggc
     4261 gtcaggcaca ccatctggcg attcagggac gcacggccgc tggtcgaggc attcgcgggg
     4321 atcgaccggc tgtacgtcgc cgacgggcat caccgctgca aggcggcgag ccgcgcggcg
     4381 gcggaagtcg agggggagga ggtccgctac ttcccggccg tcctcttccc gatgcggatg
     4441 atgcacatca tgccgtacaa ccgcgtcgtg cggcgcgtgc cgggcggcgt ggacgcgctg
     4501 gtccaggcgc tcggcgagcg ctacggcctt gagcgcgacg ccttcccgtc tcccgccagg
     4561 gcgggccgcg tgtccgtgta cgcgaaggga gcgtggctcg gtctggacct tccggcctcg
     4621 agccggccgg gcccgaccga ccggctggac gtgggtcgtc tcactgaata cgtcctggag
     4681 ccgctcctcg gcatcacgga tccgaggacc gacgcgaaca tcgacttcgt cgggggcatc
     4741 cgcggcacgg ccgaactgga gcggctcgtg gattcgggcg ccgcggaggt cgcgttctcc
     4801 atgtatccga cgagcatcgg gcagctcgtc gaggtctcgg acgcagggct tctcatgccg
     4861 cccaagtcca cctggttcga gcccaagctc cggagcggcc tgctcgtcca tacgttctga
     4921 agccgaacca cccggcctcg cccatgaaaa tcttcctcgc agacaagctg tcgtcgagct
     4981 gcgtcgacgc cctggagcgc ggcggacacg aggtcgtaag ccggccggac gcgaagggcg
     5041 actcgctcgt cgaggccctc cgcagcgaga atccggcggt cctcgtcgtg cgctccacca
     5101 aggtgcccga gtccgtcctg gacgcggccc cggcgctgga gctcgtggtg cgggcggggg
     5161 ccggatacga cacgatcgac gtcgatgcgg cctcctcgcg cgggatcttc gtgtcgaact
     5221 gcccggggaa gaactcggtc gcggtcgcgg agctcacgct gggcttggtc atggccctcg
     5281 accgcaggat ccccgacaac gtggccgatg cgaggggcgg cacatggaac aaggcggagt
     5341 actcgaaggc gcccgggctg aaggggcgaa ccctcggact ggtggggctc gggagcatag
     5401 gccgggaggt cgcccggcga gcggaggcct tcggcatgcg tgtcatcggc tggagccggt
     5461 cgctcacgcc cgaatccgcc gccgagatgg gcgtcggatt ccgcgcgtct cccctggagg
     5521 tcgcccgtga tgcggacatc gtgtcgctgc acgtcgcggc gacgcccgac acgaggcatc
     5581 tcgctgaccg gcccttcttc gaggccatga acccgggcgc ccacttcgtc aacaccacgc
     5641 gcagcagcgt cgtggacgag gaagccctgg tctgggcggt ggagaaccgg ggcatccgg
//
LOCUS       DBNHQZ010000155         1883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2077156, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000155 DBNHQZ010000000
VERSION     DBNHQZ010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2077156"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC16YU_04020"
     CDS             <1..421
                     /locus_tag="AC16YU_04020"
                     /inference="COORDINATES: protein motif:HMM:NF052219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6064421.1"
                     /translation="LVYYTDHWNLIAFDHLRGDIRNFRLDRIESLHVLTERFDKPQGF
                     DLVAHLEEQGVRPSKARIELEFSEAVWPRARVGIPARVDEESREGGMVRVAFRFDNLD
                     YVAGWMLRFGADARVVSPAELRERVRLRAAEIADLYR"
     gene            complement(455..>1883)
                     /locus_tag="AC16YU_04025"
     CDS             complement(455..>1883)
                     /locus_tag="AC16YU_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HIR6064422.1"
                     /translation="SRFRTGADSRRVVDGLADGTVDVVIGTHRLISSDVRFKSLGLLI
                     VDEEQRFGVAVKEKLRKLRVDVDTLTLTATPIPRTLQFSLLGARDLSIISTPPPNRQP
                     IVTEIHTFDKDLVRDAILYEVHRGGQVFFIHNRIQSIQDVASMIRSILPDVRVVVGHG
                     QMKGPDLERVMLQFVERKADVLVSTSIIENGLDIANANTIIIDRADHFGLSELHQLRG
                     RVGRSDRKAFCYLLVPSTQGLTREAKQRLQAVEEFSELGSGFHIAMRDMDIRGAGNLL
                     GAEQSGFIEDVGFETYHRILDEAVAELRTDEFSDLFDVRSIPRPGDTAVEIDEDALIP
                     DRYVANRIERLNLYRRLSEASAADALAGVRDEMVDRFGPLPAEVTSLLRATELRIGAQ
                     ALRLSKVAHRNDRLFLHFPDPSDDPYFFENGFHPLLARLADLDHRYVLKESKSGRLRA
                     IVQEVTDLGLALDILGRLQSEPVPA"
BASE COUNT          310 a          581 c          679 g          313 t
ORIGIN      
        1 actcgtctac tacacggacc actggaatct catcgctttc gaccacctgc gcggcgatat
       61 ccgcaatttc cgcctggatc gcatcgaatc gctgcacgtg ctgaccgagc ggttcgataa
      121 gccgcagggt ttcgacctgg tggcgcacct ggaggagcag ggcgtgcgcc cgtcgaaggc
      181 gcgcatcgag ctggaattca gcgaggcggt gtggccccgc gcccgggtag gcataccggc
      241 ccgcgtcgac gaggaatcac gggagggagg gatggtccgc gtcgccttcc ggttcgacaa
      301 cctggactac gtggcgggct ggatgctccg tttcggcgcg gacgcgcggg tggtgtcgcc
      361 cgccgagctc cgggagcggg ttcgcctgcg ggcggccgaa atcgccgatc tgtaccgatg
      421 atcgcggtgt ttcacgtgaa acatcggggc ccgttcaggc gggtacgggc tcggactgga
      481 ggcggcccag gatgtcgagc gccagaccca ggtcggtcac ttcctgcacg atagcgcgca
      541 agcggcccga tttcgactcc ttgagcacgt accggtggtc caggtcggcg agccgggcga
      601 ggagcggatg gaagccattt tcgaagaagt acgggtcgtc ggacggatcg gggaagtgga
      661 ggaagagccg atcgttgcgg tgggcgacct tggagaggcg cagcgcctgt gccccgattc
      721 ggagctccgt cgcccggagc aggctcgtga cctcggcggg aagcggtccg aagcggtcga
      781 ccatctcgtc ccggacaccg gcgagggcgt ccgctgccga cgcctcggag agccggcggt
      841 acaggttcag gcgctcgatg cggttggcca cgtagcggtc cggaatgagc gcgtcctcat
      901 cgatctccac cgccgtgtct ccaggccggg ggatggaacg cacgtcgaag agatcggaaa
      961 actcgtcggt gcgcagttcg gcgaccgcct cgtccaggat ccggtggtac gtctcgaagc
     1021 cgacgtcctc gatgaatccg ctctgctcgg cgccgagcag gttgccggct ccgcggatgt
     1081 ccatgtcgcg catggcaata tggaagccgg acccgagctc cgagaactcc tccaccgcct
     1141 gcagccgctg ctttgcctcc cgtgtgaggc cctgcgtgga cgggacgagc aggtagcaga
     1201 atgccttccg gtcggagcgg ccgacgcggc cgcgcagctg gtgcagctcg gagagcccga
     1261 agtggtcggc acggtcgatg atgatcgtgt tggcgttcgc gatgtcgagc ccgttctcga
     1321 tgatgctcgt gctcacgagc acgtcggcct tgcgctcgac gaactggagc atgacgcgct
     1381 cgagatcggg gcccttcatc tggccgtgcc cgaccacgac ccggacgtcc ggaaggatgg
     1441 accggatcat cgacgccacg tcctggatgg actggatccg gttgtggatg aagaacacct
     1501 gcccgccgcg atggacctcg tagaggatgg catcccggac caggtccttg tcgaacgtgt
     1561 ggatctcggt gacgatgggc tgccggttgg gcgggggcgt cgagatgatg gacaggtcgc
     1621 gggcgccgag gagcgagaac tgcagggtcc gggggatcgg agtggcggtg agcgtgaggg
     1681 tgtcgacgtc gacgcgaagc ttccgcagct tctccttcac ggccacgccg aagcgctgct
     1741 cctcgtccac gatcagcagg ccgaggctct tgaagcggac atcggacgag atgagccggt
     1801 gcgttccgat gaccacgtcc accgtgccgt cggccagccc gtcgacgacc cgacgcgaat
     1861 cggcccccgt gcggaaacgg gac
//
LOCUS       DBNHQZ010000156         3438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3704646, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000156 DBNHQZ010000000
VERSION     DBNHQZ010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3704646"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2179
                     /locus_tag="AC16YU_04030"
     CDS             <1..2179
                     /locus_tag="AC16YU_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064423.1"
                     /translation="PAGIICTYAVGDVAVGETKYPEFAVTVVNVVPAGAPEVSNTATV
                     ADDGTGGVDPTPADNSVTEVTPIDAEPELTVTKFDALVGDSNADGEANPGEILIYTIV
                     VANVGTQDAAGVILADIAPIHPYSELIGGSVTTTQGTVIVGNNALDTDVLIDLGTVAP
                     GAPPILVTFFVQIIDPFPPGVTEVCNQAIVDGSNFDAVSSDDPDVGGASDPTCTPVVS
                     NLAPEITSVDPASQTVQYSDAIDPITIVAVDDERPDGVAGLTLDVEWSVDGGLPTPGL
                     PAGLVVTGDPADCTVTDGKSCEWTVSGQMLQPAGSIVLSFVVSDSELESLAATASIEV
                     EAEDVAVSFDGSNEVAVQVETAGGASGPFNLVIEVEEHDDPSGLAGDIDLAGIVVTLV
                     PVGPGSPVGPVTPCTDQTVSGTADAGGSADIYDVRRLTCAFDEVPVNTYAIGVDVNLF
                     GYYHGYEEDVLVVYDPSLGFTTGGGWFYWPGTEDGDYPGDKTTFGYNIKYNRKATNTQ
                     GSAIVVRHLADDSRYRMKSNALSGLSIKNETDWGWASFAGKATYQRPNEEAEGNHEFL
                     IYVEDHGTPGVGNDRFWVRIQKDGVPVEVALPLPGDANAQIIQGGNIVVPHGGKGKGK
                     NVAGKMEFDGTEIPEVFALHPSYPNPFNPVTTLTLDVPEEGELSVTVFDILGRRVRVL
                     VDGVQAAGVYRLAFEADTLPSGLYIVVADTPVGRLTRTVTLLK"
     gene            2210..2851
                     /locus_tag="AC16YU_04035"
     CDS             2210..2851
                     /locus_tag="AC16YU_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064424.1"
                     /translation="MNRRTFHRMAGLAAGGLLVPRVVAGAWHPVLAGIPRFQLSTTPE
                     YGALDLLLDVEGPIAESAVSSTAVLPGVEHWWRIATDGQVHYYGRLQDAERLEAVADM
                     VNSTTIPFLNALHGTNVSTETEVPLEFELDQNYPNPFNPATTIPFVVDRAGPASLRVY
                     DLLGREVAVLADGDLPAGRHEAHWNASGLASGTYLAVLRSASGMRTRRLTLLK"
     gene            2891..3391
                     /locus_tag="AC16YU_04040"
     CDS             2891..3391
                     /locus_tag="AC16YU_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR6064425.1"
                     /translation="MAEPFLSEIRMMSFVFAPKGWALCNGQLLPINQNQALFSLLGTT
                     YGGDGRVNFALPDLRGRTPIHVGGGHTLGERAGEQAHTLSVAEMPTHVHAVLASQDLA
                     TSVVPGGRVLATGGANMFRDATSPTAMHVGTVATTGGSQAHLNMPPFLTLNFVIALQG
                     IFPSPN"
BASE COUNT          580 a         1186 c         1147 g          525 t
ORIGIN      
        1 gcccgcgggg attatatgca cgtacgccgt gggcgacgtg gccgtcgggg agacgaagta
       61 ccccgagttc gcggtgaccg tcgtgaacgt cgttcccgct ggtgcccccg aggtgtccaa
      121 cacggcgacc gtggccgacg acggcacggg cggcgtggac ccgacgcccg cggacaactc
      181 ggtgaccgag gtcacgccga tcgacgccga gccggagctc acggtcacca agttcgacgc
      241 gttggtcggc gactccaacg ccgacggcga ggccaatcct ggtgaaatcc tgatctacac
      301 gatcgtagtg gcgaacgtgg gtactcagga cgcggcgggc gtgatcctcg ctgacatcgc
      361 cccaatccat ccgtactcgg agctcatcgg aggctcggtc acgaccaccc aggggaccgt
      421 catcgtcgga aacaacgcgc tggacaccga cgtgctcatc gacctcggaa ccgtcgctcc
      481 gggggcccct ccgatcctgg tcacgttttt tgtccagatc atcgatccgt tcccgccggg
      541 cgtgaccgag gtgtgcaacc aggccatcgt ggatgggtcg aatttcgacg ccgtgtcgag
      601 cgacgacccg gatgtcggcg gcgcctcaga tccaacctgc acgcccgtcg tctcgaatct
      661 cgcgcccgag atcacgtccg tcgatccggc gagccagacc gtgcagtaca gcgacgccat
      721 cgatccgatc acgatcgtgg ccgtggacga cgagcggccg gacggcgtgg ccgggctcac
      781 gctggacgtc gagtggtcgg tagacggcgg cctgccgacg cccggtcttc cggccgggct
      841 cgtggtcacc ggagaccccg ccgactgcac cgtgacggac ggcaagtcct gcgagtggac
      901 cgtctccggc cagatgctcc agcccgcggg atcgatcgtg ctcagcttcg tggtctcgga
      961 ttcggaactg gagagcctgg cggccacggc ctcgatcgag gtggaggccg aggacgtcgc
     1021 cgtgtcgttc gacggctcga acgaggtcgc agtccaggtc gagacggcgg gcggcgcgag
     1081 cggtccgttc aacctcgtca tcgaggtcga ggagcacgac gacccgtccg gcctggccgg
     1141 tgacatcgac ctcgcaggga tcgtggtcac gctggtgccc gtgggtccgg gctcgccggt
     1201 cggacccgtc actccgtgca ccgaccagac cgtctccggc acggccgacg cgggcggttc
     1261 ggcggacatc tacgacgtgc gccgcctcac gtgcgccttc gacgaggtcc ccgtcaacac
     1321 gtatgccatc ggcgtggacg tgaacctgtt cgggtattac catggctacg aggaggacgt
     1381 cctcgtggtc tacgacccga gcctcggctt cacgaccggc ggcggctggt tctactggcc
     1441 ggggaccgag gacggcgact acccgggcga caagaccacc ttcggttaca acatcaagta
     1501 caacaggaag gcgacgaaca cccagggctc cgcgatcgtg gtccgtcacc ttgcggacga
     1561 ttcgcgctac cggatgaaga gcaacgccct gtccggcctg agcatcaaga acgagaccga
     1621 ctggggctgg gcgtccttcg cgggcaaggc cacctaccag cggcccaacg aggaggccga
     1681 gggcaaccac gagttcctga tctacgtcga ggatcacgga acgccgggcg tgggcaacga
     1741 ccggttctgg gtgcgcatcc agaaagacgg cgtgccggtc gaggtcgccc ttccgctccc
     1801 gggagacgcg aacgcccaga tcatccaggg cggcaacatc gtcgtcccgc acggcggcaa
     1861 gggcaagggc aagaacgtgg ccggcaagat ggaattcgac gggaccgaaa tcccggaggt
     1921 cttcgccctg cacccgagct acccgaaccc gttcaacccg gtgacgaccc tgacgctcga
     1981 cgtgcccgag gagggagagc tcagcgtgac ggtcttcgac atcctggggc gccgcgtgcg
     2041 cgtgctcgtg gacggcgtcc aggccgccgg tgtttaccgg ctcgcgttcg aagccgatac
     2101 gttgccgagc gggctctaca ttgtcgtggc ggacaccccg gtgggacgcc tcacccggac
     2161 ggtcacgctg ctcaagtagg ccccgaccac acccagccgg atactcgaag tgaacagacg
     2221 caccttccac cgcatggccg gcctcgcggc cggtggcctc ctggttcccc gggtggtcgc
     2281 cggcgcgtgg cacccggtgc tggccggcat cccgagattc cagctttcca cgacgccgga
     2341 gtacggcgcg ctggacctgc tcctcgacgt cgagggaccg atcgccgaat cggccgtctc
     2401 gtcgaccgcg gtcttaccgg gcgtcgagca ctggtggcgg atcgccaccg acggacaggt
     2461 ccactactac ggtcggctgc aggacgccga gcgcctggag gcggtggcgg acatggtcaa
     2521 ctcgaccaca atcccgttcc tgaacgcgct ccacggcacg aacgtgagca cggagacgga
     2581 ggtcccgctc gagttcgagc tcgaccagaa ctacccgaac ccgttcaacc cggcgaccac
     2641 gattcccttc gtggtcgacc gggccggacc tgcttcgctc cgggtgtacg acctgctcgg
     2701 gcgcgaggtc gccgtcctcg cggatggcga cctgccggcc ggccggcacg aggcgcactg
     2761 gaatgcgtcg ggcctggcca gcgggacgta cctcgccgtt ctccgctcgg cgtccgggat
     2821 gcgcacccgc cgcctgacgc tcctgaagta ggcgggcgaa cgactgtaaa cccaatagac
     2881 cttcagcgac atggctgagc ctttcctttc cgaaatccgg atgatgagct tcgtgttcgc
     2941 ccccaagggc tgggcgctct gcaacggcca gctcctcccg atcaaccaga accaggcgct
     3001 gttctcgctg ctgggcacca cgtacggcgg agacggccgg gtcaacttcg cgcttccgga
     3061 cctgcgggga cggacgccga tccacgtcgg cggcggccac acgctcgggg agcgcgcagg
     3121 cgagcaggcg cacacgctct cggtcgccga gatgcccacc cacgtccacg ccgtgctggc
     3181 gtcgcaggat ctcgcgacct cggtcgtgcc gggcgggcgg gtgctggcga ccggcggcgc
     3241 gaacatgttc cgagacgcga cgagtcccac ggccatgcac gtggggacgg tcgcgacgac
     3301 gggcggaagc caggcgcacc tgaacatgcc ccccttcctg acactcaatt tcgtgatcgc
     3361 gctccagggc atcttcccgt ccccaaactg agtccacccc catggcacag ccgtatgtag
     3421 gcgaaatcag gatgttcg
//
LOCUS       DBNHQZ010000157         2225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1807139, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000157 DBNHQZ010000000
VERSION     DBNHQZ010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1807139"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1158)
                     /locus_tag="AC16YU_04045"
     CDS             complement(148..1158)
                     /locus_tag="AC16YU_04045"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6064426.1"
                     /translation="MPHRWIPIALFLLASAALPTRAQLLPALGGQRAGTSGFQFLKIP
                     VDARSAALGQTAVTSAGDVSVLYWNPALAAHGPGLQAGLYHSAYFVDVTMDFAGVVWP
                     VPGTNMAVGASLQTLNSGEMDVTTEFQPFGTGETFRLLDLAVGLTFSQRLTDLFSYGV
                     TTKLVRESVAGLSASTAVVDLGFHYAVGSTGLEIAVALRNFGLDGRPEGSLSRPVIGD
                     PPSEVETSFESMTPPTTFHLGASWEALRADANQSLRVSTQLDNPSDNAETWSTGVEYG
                     WRGTLFLRTGYRFGIEEFTTPSAGVGVQVPMSGARLRFDYGFNRLERLGAVHRIGVNV
                     TL"
     gene            complement(1158..>2225)
                     /locus_tag="AC16YU_04050"
     CDS             complement(1158..>2225)
                     /locus_tag="AC16YU_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6064427.1"
                     /translation="VSFPIQENRVLFFNYGHSTRVPHPTFVYTGLDPYFQDRSFFSDL
                     GNPDLDPEVDVSYELGLRTQFSSDDVLNVAAFWRDKFDFITVENAIVKDPTGRDVIRA
                     FRINGDFARVRGLEVSYLKRVGMWFQSQLSVAYSRATGLSSTNNDALTQFLAVGDIDN
                     TFETPLAWDRPLDAKWNVILSGDRDRRLFGVPGTRRTRLYLSSTFRSGQRYTPVEFKG
                     REVNPFTGEQDWRPVYEAVSDPAKRYSEIGSAWWWFDLSLQKSVSLAGSEVVFTLEVT
                     NLFDQKNSIIVNPVTGTAYPDVDPSRVDMTALRGNPDYDVVAGTRDLRYDDPNSSGLP
                     PLNPARFLPQRHVMLGVAVQF"
BASE COUNT          392 a          698 c          784 g          351 t
ORIGIN      
        1 agagtgggac atactccact tcgttgagca gcgtgggctc gttgcggccg acctcgaaga
       61 tctcgtcggt cgtggggtcg tccttcgacc cgaagaccga gtcgcatcct gcgagggcga
      121 gcagaaggac tatcggaagg acgcgactca taacgttacg ttcacaccaa tcctatgtac
      181 ggcaccgagt cgctccagtc ggttgaagcc atagtcaaag cgtagccggg cgccgctcat
      241 cggtacctgc acgccgacac cggccgaagg ggtcgtgaat tcctcgatgc cgaagcggta
      301 tcccgtgcgg aggaagagcg tcccccgcca tccgtactcg acgcccgtgc tccaggtctc
      361 ggcgttgtcg ctcggattgt ccagctgggt cgagacacgg agggactggt tcgcgtcggc
      421 gcgcagcgct tcccaggacg cgcccaggtg gaaggtcgtc ggcggcgtca tcgactcgaa
      481 cgacgtctcg acctcgctgg gtgggtcgcc gatgaccggc cgggaaaggg agccctcggg
      541 ccggccgtcc aggccgaagt tgcgcagggc aacggcgatc tcgagcccgg tcgagccgac
      601 tgcgtagtgg aatccgagat cgacgacggc cgtcgaggca gacaatcctg ccaccgactc
      661 gcgcacgagc ttggtcgtga ccccgtagct gaagagatcc gtcaggcgct gggaaaacgt
      721 gagaccgacg gccaggtcca gcagacggaa cgtctcgcct gtgccgaagg gctggaactc
      781 cgtcgtcacg tccatttctc ccgagttcag ggtctgcagg ctggcgccca cggccatgtt
      841 cgtgcccggc acgggccaga cgacgcccgc gaagtccatc gtgacgtcga cgaagtaggc
      901 cgagtggtac agacccgcct gaaggcccgg gccgtgggcc gcgagcgccg ggttccagta
      961 caggacgctg acgtcgccgg cggacgtgac ggccgtctgc ccgagcgcgg ccgaccgggc
     1021 gtcgaccggg atcttcagga actggaaccc cgaggttccg gcacgctgtc cgccgagggc
     1081 cggcagcagc tgcgcccgcg tcggaagagc cgccgaggcg aggaggaaga gggctatggg
     1141 aatccagcgg tgcggcatca gaactgcacg gcgacgccca gcatgacgtg acgctgggga
     1201 aggaagcggg ccgggttcag cggcggcagt ccgctcgagt tcggatcgtc gtaacggagg
     1261 tcccgcgtgc cggccacgac gtcgtagtcc ggattgcccc gcagcgcggt catgtccacg
     1321 cgggacgggt ccacgtccgg ataggccgtt ccggtgaccg gattgacgat gatgctgttc
     1381 ttctggtcga agaggttcgt cacctcgagc gtgaagacga cctccgagcc ggcgagcgat
     1441 acgcttttct gcaggctcag gtcgaaccac caccaggcgg acccgatttc cgagtagcgc
     1501 ttcgcgggat cggacaccgc ctcgtagacg gggcgccagt cctgctcgcc cgtgaaggga
     1561 ttgacctcgc gtcccttgaa ctcgaccggg gtgtagcgct gcccgctccg gaacgtggag
     1621 gacaggtaca ggcgcgtccg gcgcgtcccg ggcactccga acaggcgccg atcgcggtcg
     1681 cccgagagga tcacgttcca cttggcgtcg agcggccggt cccacgcgag cggggtctcg
     1741 aacgtgttgt cgatgtcgcc cacggccagg aattgggtga gcgcgtcgtt gttcgtggag
     1801 ctgaggccgg tcgcgcgcga gtacgcgacc gaaagctggc tctggaacca catcccgacg
     1861 cgcttcaggt acgagacctc gaggccgcgt acccgcgcga agtcgccgtt gatgcggaag
     1921 gcgcggatca cgtcgcgccc cgtcggatcc ttgacgatcg cgttctcgac ggtgatgaag
     1981 tcgaacttgt cccgccagaa cgccgcgacg ttcaggacgt cgtcgctcga gaactgggtc
     2041 ctgagcccga gctcgtacga gacgtcgacc tcggggtcca ggtcgggatt gccgaggtcc
     2101 gagaagaagg accggtcctg gaagtacgga tcgaggcccg tgtagacgaa ggtcgggtgc
     2161 ggcacgcggg tcgaatgccc gtagttgaag aagagcaccc ggttctcctg gatcgggaag
     2221 gagac
//
LOCUS       DBNHQZ010000158         8967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2485054, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000158 DBNHQZ010000000
VERSION     DBNHQZ010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2485054"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC16YU_04055"
     CDS             complement(<1..262)
                     /locus_tag="AC16YU_04055"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR6064428.1"
                     /translation="MSRLSETIRKAASSGGKAMGLFVTGGFPAPGDTVPILRAIDRGG
                     ADFIELGMPFSDPLAEGLPIQHSSERALAAGASMRGTIRAAAE"
     gene            complement(264..1460)
                     /gene="trpB"
                     /locus_tag="AC16YU_04060"
     CDS             complement(264..1460)
                     /gene="trpB"
                     /locus_tag="AC16YU_04060"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR6064429.1"
                     /translation="MATPPPYDAPDASGHFGPYGGVFVPEILVPAVRELARAYDDARR
                     DPAFRTELDALLADYVGRPTPLTRADRLSSELGRGRVYLKREDLCHTGAHKINNTVGQ
                     ILLARRMGKTRIIAETGAGQHGVATATVCARFGMPCVVYMGSEDIERQHLNVLRMRLL
                     GAEVRPATSGSRTLKDATNEAIRDWVTNIRDTFYIIGSVVGPEPYPRMVRDFQRVIGL
                     ETRRQLAEAEGRETPDALVACVGGGSNAIGLFHPFLDDSGVACFGVEAAGEGLDGRHA
                     ATLTLGTPGVLHGAMSYLLQDEDGQVRIAHSISAGLDYPGVGPEHAWLRDLGRVRYLT
                     ATDEDALFGVRLLARTEGIIPALETAHAVGILPRLVRELDPDAVLVVNCSGRGDKDMG
                     TIASHV"
     gene            complement(1468..2109)
                     /locus_tag="AC16YU_04065"
     CDS             complement(1468..2109)
                     /locus_tag="AC16YU_04065"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR6064430.1"
                     /translation="MMKIKICGITTLEDARFCAAAGADYLGFIQYEESPRYLSAGDAR
                     AIIEWLYGPQPVGVFVDTHPDAVNAAADRAGFALVQLQGDEPPEDCAAVERPVIKAIG
                     IRPGMTADDVRRTASRYEGAADYLLLDTRAGGLHGGTGIAFDWSLAAGLAPGTPFFLA
                     GGLRPENVAEAASLVRPFAVDVASGVEEAPGRKDFVRVQAFFDAMAPFRAEKT"
     gene            complement(2103..2909)
                     /gene="trpC"
                     /locus_tag="AC16YU_04070"
     CDS             complement(2103..2909)
                     /gene="trpC"
                     /locus_tag="AC16YU_04070"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR6064431.1"
                     /translation="MAAILDTILRATRDLVRARRASVSMRELEQHELFAEPRRGLARA
                     LRGERLAVIAECKKASPSAGVIREDYDVAAIARAYARAGADAISVLTEPSFFQGEVDH
                     LDLARRAAGVPLLRKDFVVDPFQIAEARAHGADAVLLIATALDRHELVDLMDAADALE
                     MEYLVECHAERELDRVDLDRVRILGVNNRNLDTFEVDPGHAARVLARVPASIVRVAES
                     GLRTPDDLAAARRDGIDAVLVGEALMRADDPGRALELLRNGGPPSPAPES"
     gene            complement(2909..3943)
                     /gene="trpD"
                     /locus_tag="AC16YU_04075"
     CDS             complement(2909..3943)
                     /gene="trpD"
                     /locus_tag="AC16YU_04075"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6064432.1"
                     /translation="MPLKAALTRLASGRELTVDEASQAMHVLMQGKAAPEQVAGLLMG
                     LRSRGESLDELVTFTGVMREYAVRVRVDDPAAIDIVGTGGDAGGTFNISTAAMLVCAG
                     AGATVAKHGNRSVSSKCGSADVLEALGVRIDLQAPGVEACLRDVGMGFMFAPLFHPAL
                     RNVMPVRRALAVRTFFNILGPLCNPAFVTRGVFGAFSRDVAETSAEILSRLGAAHVVA
                     VHSEDGLDEFSLSAPSMAFEQVGTEAPVVRTVRAEDLGLGSFGLDAIRGGDAAENAGI
                     IRGVLDGRPGPHRDVVLLNAAHGLVAAGLASDAAEGVGLAAESIDSGRAARKLADLVD
                     ATRAHAEDIG"
     gene            complement(3954..4538)
                     /locus_tag="AC16YU_04080"
     CDS             complement(3954..4538)
                     /locus_tag="AC16YU_04080"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR6064433.1"
                     /translation="MILVIDNYDSFTYNLVHLVAGSGVPVRVERNDAVSVDGIAAMRP
                     AGILVSPGPGRPADAGITEEVIRRLGERIPVLGVCLGHQAIGEAYGGRVVHAPTLMHG
                     KTSRIRHDGRSVFRGVEQDFIATRYHSLVVDRPTLPAELEVTAWTADGVVMGLRHRAH
                     PVEGIQFHPESLLTTVGPTLVQNWLDSLPAVIEA"
     gene            complement(4535..5554)
                     /gene="trpS"
                     /locus_tag="AC16YU_04085"
     CDS             complement(4535..5554)
                     /gene="trpS"
                     /locus_tag="AC16YU_04085"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6064434.1"
                     /translation="MPHPKPASRPVVVSGIQPSGTLHLGNYFGALRQHIALHREHESY
                     YFIVNYHAMTTLQDADMLRKYSLDVGLDYLALGFDPDHAHLFLQSDVPAVTELAWILQ
                     TITPVSQLEKGVAYKDKVAQGLGANAGLFSYPVLQAADILIYGGELVPVGADQKQNIE
                     ISRDLAQRFNQTFSPDSPVFPLPEPLILDDVAVVPGVDGRKMSKSYGNTIGIFDEGAE
                     LKQKVAGIVTDSTPLEAPKDPDRCNVFALIRLFAGDEDRQRIADAYRAGGYGYGHAKK
                     ELRGMIQGYFAEARERRRDLARRPDDVMDILREGGRAGRERAAVFMEAVRSVTGLVTT
                     YPAAG"
     gene            complement(5559..6008)
                     /locus_tag="AC16YU_04090"
     CDS             complement(5559..6008)
                     /locus_tag="AC16YU_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064435.1"
                     /translation="MGIKDLLLNKGWAGTTLSRSKTVDRINPVIESLTTLMHGYEAAK
                     GALPAEAAASVDDALKLLRGDLDKLSETVLSCGGVAYSGVDQEPPSPAGGEASVAAND
                     LLRQEEAFRSVLEGERKVEHQIRTRAVLENVSRNSSERMRLLRDLAR"
     gene            complement(6076..7572)
                     /gene="trpE"
                     /locus_tag="AC16YU_04095"
     CDS             complement(6076..7572)
                     /gene="trpE"
                     /locus_tag="AC16YU_04095"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR6064436.1"
                     /translation="MPDPRTRFLDQVLAMRREGTERFVLPVHRRLSADLVTPVSAFLA
                     LREGARHAFLFESVEGGEKLARYSFLGRDPYRLFEARGDAVERIDPGSGQRSRVEGNI
                     FEVLRRELSRWREVREPDLPRFTAGAVGYAAYDTVRLIERLEHAPPDDLGTPDGIWGL
                     YDTVAAFDHVRHQLVLMASAFIEPETDPEAAFDDAERRIALLAERLSAPVSHGPETVE
                     LTGPMTSNFERAAFVEAVERAKRYIYEGDIFQVVLSQRFRLPYRGDRFNLYRALRQVN
                     PSPYLFHLDFDGFSLVGSSPEVLVRVENGRAEVLPIAGTRPRGDTARADADLERDLLD
                     DPKERAEHLMLVDLGRNDLGRVCEFGSVSVDRYAFVERYSHVMHIVSSVSGRLAPTSD
                     AVDALAACFPAGTVSGAPKVRAMEIIDELEPTRRGPYAGAVGYLDFSGNLDTCITIRT
                     MLVRDDAIFVQAGAGIVADSDPAREFEETENKALALRQAILLAARDLL"
     gene            complement(7733..8671)
                     /locus_tag="AC16YU_04100"
     CDS             complement(7733..8671)
                     /locus_tag="AC16YU_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6064437.1"
                     /translation="MQLPFEIGDELLTRMALILVTLVVLVLVMRLAQRIARRTVQDPA
                     RVYRANRIIRRLVAVAAVVLVIMLLSPDLRALVTVLTVIGAGLAIALREALLSVAGWI
                     RIVLMSPYSVGERVQIGDVHGDVIDIRMLRTTLMEIRGWVEADQSTGRIVHVPNSWVF
                     ERAVYNYSRGFRFIWNEVSFTITFGSDWRAARDIILGLAEESSSIVESQAAREIREMS
                     REFLVHYGILTPFVYVQVVENGVRLTLRYLCEVRKRRGTEHAFTVSILDAFREHGGIE
                     FAYPQIEISTPGQSQFSPPVPPADGGPGLAAAHPDA"
     gene            complement(8684..>8967)
                     /locus_tag="AC16YU_04105"
     CDS             complement(8684..>8967)
                     /locus_tag="AC16YU_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6064438.1"
                     /translation="YTGLVVLDEVQSLLASTLAMQHLIVAHDIQSPFAFVPADGTLED
                     ARAAFERTGYPELPVMDQGQITGFIRKAQLVSEYHRAYLRTRSAFEPEQ"
BASE COUNT         1342 a         3100 c         3197 g         1328 t
ORIGIN      
        1 attcggccgc cgcccggatc gtgccccgca tcgaggctcc cgccgcgagc gcccgctcgc
       61 tcgagtgctg gatcgggagg ccctcggcaa gcgggtcgct gaagggcatg ccgagctcga
      121 tgaagtcggc tcccccgcgg tcgatggccc gcaggatcgg gaccgtgtcc cccggcgcgg
      181 ggaatccgcc cgtcacgaag agccccatgg ccttgccgcc cgaggacgcc gccttccgta
      241 tggtctccga gagtcggctc atgtcatacg tgcgaggcga tcgtgcccat gtccttgtct
      301 ccccgtccgg agcagttcac gaccaggacg gcgtccggat cgagctcgcg gaccaggcgc
      361 ggcaggatgc ccacggcatg ggcggtctcg agcgcgggga tgatgccctc cgtgcgcgcg
      421 aggagccgga cgccgaagag cgcgtcctcg tcggtggccg tgagatagcg tacccgtccg
      481 aggtcccgga gccacgcgtg ctcggggccg acgcccgggt aatccaagcc ggccgagatg
      541 gagtgtgcga tccggacctg gccgtcctcg tcctgcagca ggtagctcat ggcgccgtgg
      601 aggacacccg gggttccgag cgtgagcgtc gccgcgtgcc ggccgtcgag gccctcgccg
      661 gccgcctcca ccccgaagca ggccacgccc gaatcgtcca ggaacggatg gaacagcccg
      721 atggcgttcg atcctccgcc cacgcacgcg acgagcgcgt ccggggtttc gcggccctcg
      781 gcctcggcga gctgccggcg cgtctcgagc ccgatgacgc gctggaagtc ccggaccatc
      841 cggggatagg gctccgggcc gaccaccgag ccgatgatgt agaaggtgtc gcggatgttc
      901 gtgacccagt cgcggatggc ctcgttggtc gcgtccttga gcgtgcggct tccgctcgtc
      961 gccggccgga cctccgcgcc caggagccgc atccggagca cgttcaggtg ctgccgctcg
     1021 atgtcctccg agcccatgta caccacgcac ggcatgccga agcgggcgca gacggtcgcc
     1081 gtggccacgc cgtgctgccc ggcgccggtc tcggcgatga tgcgcgtctt gcccatacgg
     1141 cgggcaagca ggatctggcc caccgtgttg ttgatcttgt gcgcccccgt gtggcacaga
     1201 tcctcccgct tcaggtacac gcggccgcgt ccgagctcgg acgagaggcg gtccgcacgg
     1261 gtgagcggcg tgggccggcc gacgtagtcc gccagcagcg cgtcgagctc cgtccggaag
     1321 gcggggtccc gacgggcatc gtcgtaggcg cgcgcaagct cgcggaccgc cggtaccagg
     1381 atctcgggca cgaacacgcc gccgtacggc ccgaagtggc cgctcgcgtc cggcgcgtcg
     1441 taggggggag gcgtggccat cgtcgattca ggtcttctcg gcacggaacg gggccatggc
     1501 gtcgaagaag gcctgtacgc gcacgaagtc cttccgtccc ggcgcctcct cgactccgct
     1561 cgcgacgtcc accgcgaagg gccggacgag cgaggcggcc tcggcgacgt tctccgggcg
     1621 aaggccgccg gccaggaaga acggcgtgcc cggcgcgagg ccggcggcca ggctccagtc
     1681 gaaggcgatt cccgtgccgc cgtgcagccc gccggcgcgc gtgtccagga gcaggtagtc
     1741 ggcggccccc tcgtaccggg acgcggtacg ccggacgtcg tcggccgtca tgcccgggcg
     1801 gatcccgatc gccttgatga ccggccgctc gacggccgcg cagtcctcgg ggggctcgtc
     1861 gccctggagc tggaccagtg cgaagcccgc ccggtcggcg gcggcgttca ccgcgtcggg
     1921 atgggtgtcg acgaacacgc ccaccggctg cggaccgtac agccattcga tgatggcccg
     1981 ggcatcgccc gcggaaaggt atcgcgggct ctcctcgtac tggatgaaac cgaggtagtc
     2041 ggcgccggcc gcggcgcaga agcgtgcgtc ctcgagggtg gtgatgccgc agatcttgat
     2101 cttcatgatt ccggggccgg ggatggaggg ccgccgttcc ggaggagctc cagcgccctc
     2161 ccgggatcgt ccgcccgcat gagcgcttcg cctacgagca ccgcgtcgat gccgtcccgc
     2221 cgggcggccg cgaggtcgtc cggggtccgc aggccgctct cggccacgcg cacgatcgat
     2281 gcggggacgc gggcgagcac gcgcgcggcg tggcccgggt ccacctcgaa ggtgtccagg
     2341 ttccggttgt tcacgccgag gatgcgcacc cggtccaggt ccacgcggtc gagctcgcgc
     2401 tccgcgtggc attccacgag gtactccatc tccagggcgt cggcggcgtc catgagatcg
     2461 accagttcgt gccggtcgag cgcggtcgcg atcagcagca cggcgtccgc gccgtgcgcg
     2521 cgtgcctccg cgatctggaa cggatccacg acgaagtcct tccggaggag cggcacgccg
     2581 gcagcccgcc gggcgagatc caggtggtcg acttccccct ggaagaagga cggctcggtg
     2641 agaacggaga tcgcgtcggc gcccgcgcgc gcgtacgccc gggcgatggc cgcgacgtcg
     2701 tagtcctcgc ggatgacgcc ggccgacgga gatgccttct tgcattcggc gatgacggcc
     2761 aggcgctccc cacgcagtgc ccgggcgagg ccgcgccggg gctccgcgaa cagctcgtgc
     2821 tgctccagtt cgcgcatgct caccgatgcg cggcgcgccc ggaccaggtc gcgcgtggcg
     2881 cggaggatgg tgtcgaggat cgccgccatc acccgatgtc ctcggcgtgc gcccgggtgg
     2941 cgtccacgag gtcggcgagc ttgcgggcgg cccgtccgga gtcgatgctc tcggcggcga
     3001 ggccgacgcc ttcggcggcg tcggaggcca ggcctgcggc gacgagcccg tgcgccgcgt
     3061 tcaggagcac cacgtcacgg tgcgggcccg gacgaccgtc gagcacgccc cggatgatcc
     3121 cggcgttctc ggcggcgtcg cctccccgga tcgcgtcgag cccaaaggac ccgagcccca
     3181 ggtcctccgc gcggaccgtc cggacgactg gggcttccgt tcctacctgc tcgaaggcca
     3241 tcgacggcgc cgaaagggag aactcgtcca ggccgtcctc cgagtggacc gccaccacgt
     3301 gcgccgcgcc gagccgggac aggatctcgg cgctcgtctc ggcgacgtcc cggctgaagg
     3361 cgccgaacac gcccctcgtc acgaaggcgg gattgcagag cggtcccagg atgttgaaga
     3421 acgttcgcac cgcgagcgcc cgcctcacgg gcatgacgtt gcggagcgcc gggtggaaga
     3481 gcggggcgaa catgaagccc atgcccacgt cccgcaggca tgcctcgacc cccggcgcct
     3541 gcaggtcgat ccggacgccc agggcctcga gcacgtcggc cgagccgcac ttcgaggaga
     3601 ccgagcggtt gccgtgcttg gccacggtgg cgccggcgcc cgcgcacacg agcatggccg
     3661 ccgtcgagat gttgaacgtg cctccggcgt cgccccccgt cccgacgatg tcgatcgccg
     3721 ccgggtcgtc gacccgcacg cgtacggcgt actcgcgcat gacgccggtg aaggtcacga
     3781 gctcgtccag actctcgccg cggctcctga ggcccatcag gagccccgcg acctgttccg
     3841 gggccgcctt gccctgcatc aggacgtgca tcgcctgcga ggcctcgtcg accgtcagct
     3901 cccggccgga ggccagtcga gtgagcgcgg ctttcagcgg catgtcggtc ccctcaggct
     3961 tcgatgacgg cgggaagcga gtcgagccag ttctgcacga gcgtcggccc gacggtcgtg
     4021 agcaggctct cgggatggaa ctggatgccc tcgacagggt gggcgcggtg gcgcaggccc
     4081 atgacgaccc cgtccgccgt ccatgccgtg acctcgagct cggccgggag cgtgggccgg
     4141 tccacgacca gcgagtgata gcgcgtggcg atgaaatcct gctccacgcc gcggaacacg
     4201 gaccggccgt cgtgccgaat gcggctcgtc ttcccgtgca tgagggtcgg cgcgtgcacg
     4261 acgcgcccgc cgtacgcctc gccgatcgcc tggtggccca ggcatacccc gaggacgggt
     4321 atgcgctctc ccaggcgccg gatgacctct tccgtgatgc cggcgtccgc cgggcggccg
     4381 ggtcccggcg agacgaggat gcctgcgggc cgcatcgcgg caatcccgtc cacggagacg
     4441 gcgtcgttcc gctcgacgcg aaccggcacg cccgagcccg caaccaggtg gaccaggttg
     4501 taggtgaagg aatcgtagtt gtcgatgaca aggatcatcc cgcggccgga taggtggtca
     4561 cgagtcccgt cacggagcgc acggcctcca tgaagacggc cgcccgctcc cgccccgcgc
     4621 gcccgccctc gcgcaggatg tccatcacgt cgtcgggacg gcgggcgaga tcgcgccggc
     4681 gctcgcgggc ctccgcgaaa tacccctgga tcatgccacg gagctccttc ttcgcgtgcc
     4741 cgtagccgta accgcccgcg cggtacgcgt ccgcgatgcg ctgccggtcc tcgtcgcccg
     4801 caaagaggcg gatgagcgcg aagacgttgc agcggtccgg atccttggga gcctcgagcg
     4861 gcgtggagtc ggtcacgatg cccgccacct tctgtttgag ctcggccccc tcgtcgaaga
     4921 tgccgatcgt gttgccgtag ctcttggaca tctttcgtcc gtccacaccg ggcacgacgg
     4981 ccacgtcgtc cagaatgagc ggctcgggga ggggaaacac cgggctgtcc gggctgaagg
     5041 tctggttgaa gcgctgcgcg aggtcccggc tgatctcgat gttctgtttc tggtccgcgc
     5101 ccaccgggac cagctcgccg ccgtagatca ggatgtccgc cgcctggagc accgggtagc
     5161 tgaacaggcc cgcgttcgcc cccagtccct gggcgacctt gtccttgtac gcgacgccct
     5221 tctccagctg cgagaccggc gtgatcgtct gcaggatcca tgccagctcg gtgacggcgg
     5281 gaacgtcgct ctgcaggaag agatgggcat ggtccgggtc gaagcccagt gccaggtagt
     5341 cgaggcccac gtccagcgag tacttccgga gcatgtcggc atcctggagc gtcgtcatcg
     5401 cgtggtagtt cacgatgaag tagtacgatt cgtgctcccg gtgcagcgcg atgtgctggc
     5461 ggagtgcgcc gaagtagttg cccaggtgca gcgtgcccga cggctggatg ccggacacga
     5521 cgacggggcg ggaggcgggc ttcgggtgag gcatcaggct accgggccag gtcgcgcagc
     5581 agccgcatcc gttccgagct gttccgcgac acgttttcga gcacggcgcg ggtccggatc
     5641 tggtgctcca ccttgcgctc tccctccagg acggagcgga acgcctcctc ttgccggagg
     5701 agatcgttcg ctgcgacgga cgcctcgccc ccggcggggg agggcggctc ctggtcgacg
     5761 ccggaatacg ccacgccccc gcagctcagg acggtctcgg aaagcttgtc caggtcgccg
     5821 cgcaggagct tcagggcgtc gtcgaccgag gcggccgcct cggccgggag cgcccctttc
     5881 gcggcctcgt agccgtgcat gagggtggtg agggactcga ttaccggatt gatgcgatcc
     5941 acggtcttgg accgggacag cgtggtgccg gcccaaccct tgttcagaag cagatccttg
     6001 attcccatgg ctaagccgct tcgttcggtg tggaaaggga cgtgaacggt cgaggagggg
     6061 tgaagttgtc gatggtcaga gcaggtcgcg ggcggcgagc aggatggcct gccgcagggc
     6121 cagcgccttg ttctcggtct cctcgaactc ccgggcggga tcgctgtcgg ccacgatgcc
     6181 cgctccggcc tgcacgaaga tcgcatcgtc gcggacgagc atggtccgga tggtgatgca
     6241 ggtgtcgaga tttcccgaaa aatcaagata tccgacggcc ccggcatacg gaccgcgccg
     6301 ggtcggctcg agctcgtcga tgatctccat cgcgcggacc ttgggagcgc cgctcacggt
     6361 gcccgcgggg aagcaggcgg ccagcgcgtc caccgcgtcg gacgtgggcg caaggcggcc
     6421 cgacacggac gacacgatgt gcatgacatg ggagtaacgc tcgacgaagg cgtaccggtc
     6481 cacgctcacc gatccgaact cgcacacgcg tccgaggtcg ttgcgcccga ggtccacgag
     6541 catgagatgc tccgcgcgct cctttggatc gtccaggagg tcccgctcca ggtccgcgtc
     6601 cgcccgggcc gtgtcgcccc ggggccgcgt gccggcgatg ggcaggacct cggcccggcc
     6661 gttctcgacg cgcacgagca cctccgggga cgagcccacg agcgagaagc cgtcgaaatc
     6721 caggtggaac aggtagggcg aggggttcac ctggcggagc gcccggtaca ggttgaagcg
     6781 gtcgccccgg tacggcaggc ggaatcgctg cgagaggacg acctggaaga tgtcgccctc
     6841 gtagatgtag cgcttggccc gctcgacggc ctccacgaac gccgcgcgct cgaagttcga
     6901 ggtcatgggg ccggtgagct ccacggtttc gggcccgtgc gaaacgggag ccgagaggcg
     6961 ctcggcgagg agggcgatcc ggcgctcggc gtcgtcgaat gccgcttccg ggtcggtttc
     7021 gggctcgatg aaggccgacg ccatgaggac gagctggtgc cgcacgtggt cgaaggctgc
     7081 cacggtgtcg tacaggcccc agatcccgtc gggggtgccc aggtcgtccg ggggcgcatg
     7141 ctcgagcctc tcgatcagcc ggaccgtgtc gtacgcggcg tatcccaccg cgccggccgt
     7201 gaagcggggc aggtccggct cgcgcacctc ccgccagcgc gagagctcgc ggcgaagcac
     7261 ctcgaagatg ttgccctcga cccggctccg ctgccccgat ccggggtcga tccgctcaac
     7321 cgcgtcgccc cgggcctcga acaggcggta gggatccctc cccaggaacg agtaccgggc
     7381 gagtttctcc ccgccctcca cgctttcgaa caggaacgcg tggcgcgcgc cctcgcgaag
     7441 cgccaggaag gccgagacgg gggtgacgag gtccgcgctc agccggcggt gaaccgggag
     7501 cacgaaccgc tcggtgcctt cccgccgcat ggcgagcacc tggtccagga agcgggtgcg
     7561 tggatcgggc atatgggcaa caaaaaagcc cgcctctttc tcgaagcggg ctgcacggcc
     7621 ggtctgttgt gacgcggtaa tcgttcgttc gtcacgcacc ggggcggccc gcatgggcgc
     7681 cccaccacca tgagcggatg atcgtgtgcg tggcgaattc catggggcga agctacgcgt
     7741 ccggatgggc cgccgcaagc cccggtcctc cgtccgccgg gggaacgggg ggactgaact
     7801 gcgactgccc gggcgtcgag atctcgatct gcgggtaggc gaactcgatg cccccgtgct
     7861 cgcggaacgc gtcgaggatg ctcaccgtga acgcgtgctc cgtcccgcgc cgcttgcgga
     7921 cctcgcacag gtagcgcagc gtcaggcgga ccccgttctc cacgacctgc acgtacacga
     7981 acggcgtcag gatgccgtaa tggaccagga actcgcggga catctcccgg atttcccgcg
     8041 ccgcctgcga ctccacgatg gacgaggact cctcggccag tcccaggatg atgtcccggg
     8101 ccgcgcgcca gtcgctaccg aacgtgatcg tgaacgagac ctcgttccag atgaagcgga
     8161 agccgcgcga atagttgtag acggctcgct cgaacaccca gctgttgggc acgtgcacga
     8221 tgcgccccgt ggactggtcc gcctcgaccc acccgcggat ctccatgagg gtcgtgcgga
     8281 gcatccggat gtcgatcacg tcgccgtgga catccccgat ctgcacgcgc tccccgacgc
     8341 tgtacgggga catcaacacg atccggatcc acccggccac gctgaggagc gcctcgcgga
     8401 gcgcgatggc gaggccggcg ccgatcaccg tgagcaccgt gacgagcgca cgcaggtcgg
     8461 gcgagaggag catgatcacg agcacgacgg ccgcgaccgc gacgaggcgc ctgatgatcc
     8521 ggttcgcccg gtacacccgg gccggatcct gcaccgtgcg ccgggcgatg cgctgcgcga
     8581 gccgcatcac caggacgagc accacgagcg tgaccaggat cagggccatc ctggtcagga
     8641 gttcgtctcc gatttcgaag ggaagctgca tggaagcccc gtcttactgc tccggctcga
     8701 atgcggaccg ggtccgaagg taggcccggt ggtactcgga gacgagctgt gccttgcgga
     8761 tgaagcccgt gatctgcccc tggtccatga ccggcagctc gggatacccc gtccgctcga
     8821 acgccgcgcg ggcgtcctcc agcgtgccgt cggcggggac gaaggcgaac ggactctgga
     8881 tgtcgtgggc gacgatcagg tgctgcatcg caagggtgga cgccaggagg ctctgaacct
     8941 cgtcgaggac gaccaggccc gtgtagg
//
LOCUS       DBNHQZ010000159         7570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1807794, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000159 DBNHQZ010000000
VERSION     DBNHQZ010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1807794"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="AC16YU_04110"
     CDS             complement(<1..1403)
                     /locus_tag="AC16YU_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6064439.1"
                     /translation="MRIRPLLLLLPTLLAAAPAAGQTFTTDDPVIRAMWRVGMEESRT
                     EDLAHVLMDRIGPRLAGSAALGQAQDWLVETYGSWGVSARREAVGEWTGWEAGALHVD
                     LVAPRVATLEAEFLAWSPGTDGPVEAEVVLPPPGLSETGAADWLGSVSGKFVLMSAPE
                     PMCRAHQELEANARPETVERVNAERDALRRSFEERFAPIADIRRREAVVDSAGAAGLV
                     SSRWSGGWGVNKVFSTNARQAVAIDLSCEDYGLLYRLVEHGETPVLRVRAEASVLGAV
                     PQFNVVAEIPGSEKPDEYVLLGAHLDSWHAGTGATDNGTGTITMLEAMRILRETYPNP
                     KRTILAGHWANEEMGLIGSGAFREDHPEVMAGLQVAFNQDNGTWRFERIEGQGFLSSA
                     DFLPRWMARVPTDFSSRILVEVPGPQANMGSDHSSFVCAGAPSFRLQSPYDEYRQYTW
                     HTNRDTYDKIVFDDLKE"
     gene            complement(1469..3082)
                     /locus_tag="AC16YU_04115"
     CDS             complement(1469..3082)
                     /locus_tag="AC16YU_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR6064440.1"
                     /translation="MRLLFALAAALPVLAPSPDALAQDSALRFEVSFPASVEAAPITG
                     RVFLAFAADDDPEPRLQVGRYGVPFFGIDVEALAPGKAVVFDATVAGYPPESLGELPE
                     GDYVVQAIVNRYTEFRRSDGHVVWMHDDRWEGQDWRRSPGNLVSEPVAVRVGPGASGT
                     VALSADRVLPAVDVPADTEWVKRFRIRSEILSTFWGRDLWLGMTVLLPHGYGDHPDAH
                     YPTLYQQGHFSLRPPLGFREGSDVHAAWTGEGFPRFIAVTIQHATPYFDDSYAVNSVN
                     NGPYGDAIHRELIPEFERRFRAIPEGWARVLTGGSTGGWESFALQVFYPDFYGGSWAF
                     SPDPLDFRNVEGINIYEDANAFHKEYDWYKVPTVNTRDHVTGDVRLTSRQRNRMELVN
                     GTRGRSGEQLDIWSATFGPVGDDGYFRPLFDKETGEIDPEVAAYWRENYDLRHILERD
                     WATLGPKLVGKLHTGVGLMDNFFLDFGVYHMEEFLESTTDPYYAGEFWYGRRGGHGWR
                     PFDTVELVRRMADHVHANAPAGADLDWLH"
     gene            3261..4691
                     /locus_tag="AC16YU_04120"
     CDS             3261..4691
                     /locus_tag="AC16YU_04120"
                     /EC_number="1.13.12.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR6064441.1"
                     /translation="MTQAIALPLPLIIQGGMGVGVSDWRLARAVSLLGQLGVVSGTAI
                     DSVVARRLQDGDLGGHVRRALDAFPFREVAEEAMRRFFRPSGRDEGTSYTGVPMFRIG
                     ANRFRDLFTVLANFVEVHLAKEGHDGSVGVNYLTKIQMPTLPSLYGAMLAGVDFVLMG
                     AGIPREIPGALDALSRHEPAHLKLDIDGKADPEADVLSFDPAAVGLTGTEPLKRPRFL
                     PIIASNSLATMMLRRANGQVDGFIIEGPTAGGHNAPPRGKKVYNEKGEPLYGERDVVN
                     LEEIAELGLPFWVAGGTGRPGALEEVRSRGATGIQVGTLFAFCDESGLTEEYRREVVR
                     EALRHSVEVRTDDRASPTGFPFKTVGMARSLSDDAVYAARTRICDLGYLRVPVRLDDG
                     KVEYRCPAEPEADWERKGGDAEVTEGRRCLCNALMANIGLGQQRSDGTSEPPLLTSGD
                     QLAELGRFLDGRERYSASDVLDYLLG"
     gene            complement(4700..5767)
                     /locus_tag="AC16YU_04125"
     CDS             complement(4700..5767)
                     /locus_tag="AC16YU_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6064442.1"
                     /translation="MKDTLTFALLVACLAPLPACSLFDDDDSDKVRVMTRNVSVGGDI
                     DRIPASASQEDVPLNVLLTWQEIVSNDFAVRAEALAAEIDARNPHIVGLQEITTLRVQ
                     DPGDMAFGGMVPATEIAYDYLQILLDALEDRGLDYEVAGVVQDTDAEVPMVVSTDPLG
                     FWDIRWTDFDAVLVKSSVEVTDVETANYEVALEVDLGGLLFRVERGYVAFDAAVGDHE
                     FRFVSTHLEPAPASQSRLIQAAQANELLGVLGDETTILVGDLNTGPDDSTYGLFAGGG
                     FSDAWVDVHGANPGLTCCFAPNLLPGRELDTRYDLVLYRNGPATRLTPEAAVITGTGV
                     IADAGYYPSDHAGVTVRFGAD"
     gene            complement(5865..6971)
                     /locus_tag="AC16YU_04130"
     CDS             complement(5865..6971)
                     /locus_tag="AC16YU_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6064443.1"
                     /translation="MKPNPMMLLVLACLLSVFACDTLGGQDAVVERSDLEIGAKRPGE
                     VRVMTRNVAIGGDIDRVLAAPSMDEVPLLVAQTWQEIVTNDFHVRAEALAQEVKAHGP
                     DIIGLQEITTLMIQDPGDMVMGGAEPATDVVYDFLQILQDALEARGLDYVVVGVVADT
                     EAEMPMVTSADPTFADVRMIDYDAVLARADVGIENVLAQNFAYDWSLPFGPGGLTVKR
                     GYVAFDAQVGEASFRFVSTHLEPVAAPDLQPLQLGQALEIVAVAGEGTAIVVGDLNTA
                     PGDPAYELLTSSGLLDSWAVLPGEAPGLTCCFAPDLVPGRVPDLRYDLVLYRNGGTTQ
                     LAPARGVITGRDPIEGVGIYPSDHAGVVIAFGAD"
     gene            complement(7074..>7570)
                     /locus_tag="AC16YU_04135"
     CDS             complement(7074..>7570)
                     /locus_tag="AC16YU_04135"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6064444.1"
                     /translation="AVAAGAARPMHVFAMELLQNTPVRAETQTRIAAPPSIVWEVLTD
                     LERWPDWNPDVRSIKLEGPLAVGTTFRWKSGLASIRSTIRVLEPRRIIAWSGEAAGIR
                     AVHVWRISADRAGTLVWTGETFEGILAVLLRRRLERRLAEALDQGIVALRAECERRGA
                     EAAA"
BASE COUNT         1156 a         2616 c         2665 g         1133 t
ORIGIN      
        1 ttctccttca ggtcgtcgaa cacgatcttg tcgtacgtgt cccgattcgt gtgccaggtg
       61 tactggcggt actcgtcgta gggcgactgc aggcggaagc tcggcgcccc ggcgcacacg
      121 aaggacgagt ggtcgctgcc catgttggcc tgggggccgg gcacctcgac cagtatgcgc
      181 gacgagaaat cggtaggcac gcgggccatc cagcgcggga ggaaatcggc cgacgacagg
      241 aagccctgtc cctcgatgcg ctcgaagcgc caggtcccgt tgtcctggtt gaaggcgacc
      301 tgcaggcccg ccatcacctc gggatggtcc tcgcggaacg cccccgagcc gatgagcccc
      361 atctcctcgt tcgcccagtg ccccgcgagg atggtccgct tcgggttcgg gtacgtctcg
      421 cgcaggatgc gcatggcctc gagcatcgtg atcgtaccgg tgccgttgtc cgtggcgccg
      481 gttccggcgt gccagctgtc caggtgcgcg ccgagcagca cgtactcgtc gggcttctcg
      541 gagccgggaa tctctgccac gacgttgaac tgcggcacgg cgccgagcac gctggcctcg
      601 gcccggacgc gcagcacggg cgtctcaccg tgctcgacga gccggtacag caggccgtag
      661 tcctcgcaag aaaggtcgat ggccacggcc tgccgcgcgt tcgtcgagaa caccttgttc
      721 acgccccagc ccccggacca gcgcgacgag acgaggcccg ccgcgcccgc gctgtcgacg
      781 accgcctcgc gccggcggat gtccgcgatc ggggcgaagc gctcctcgaa cgagcgtcgg
      841 agcgcatcgc gctcggcgtt cacgcgctcg accgtctccg gccgcgcgtt cgcctcgagc
      901 tcctggtgcg cgcggcacat cggctcgggc gcggacatca gcacgaactt gccgctcacc
      961 gatccgagcc agtcggccgc tccggtctcc gagagccccg gagggggcag gacgacctcg
     1021 gcctccacgg gtccgtcggt gccggggctc caggccagga attccgcctc cagcgtggcc
     1081 acgcgcgggg ccacgaggtc cacgtgcagc gcgcccgcct cccagccggt ccactcgccg
     1141 accgcctctc gacgcgcgga aacgccccac gagccgtacg tctcgacgag ccagtcctgg
     1201 gcctggccga gggccgcgct gcccgcgaga cgcggaccga tgcggtccat caggacgtgc
     1261 gccaggtcct ccgtccggga ttcctccatg ccgacgcgcc acatggcccg gatcaccggg
     1321 tcgtcggtcg tgaaggtctg gccggccgcc ggcgcggcgg cgagcagggt cgggaggaga
     1381 aggagaagcg ggcggatgcg catcggatcg cggtcttcct tgagggccga aaggtagggc
     1441 ctgagcgcgc ggcgcggaag aaaggccgtc agtgcagcca gtccaggtcc gccccggccg
     1501 gcgcgttcgc atgcacgtgg tcggccatgc ggcgcacgag ttcgaccgtg tcgaacggtc
     1561 gccacccgtg tccgccccgc cggccgtacc agaactcccc ggcgtagtac gggtcggtcg
     1621 tggactcgag gaactcctcc atgtggtaca ccccgaaatc gaggaagaag ttgtccatga
     1681 ggccgacgcc cgtgtgcagc tttccgacga gcttgggtcc gagcgtcgcc cagtcgcgct
     1741 ccaggatgtg cctcaggtcg tagttctcgc gccagtacgc ggcgacctcc gggtcgatct
     1801 cgcccgtctc cttgtcgaag agcggcctga agtagccgtc gtccccgacg ggcccgaacg
     1861 tcgcgctcca gatgtcgagc tgctcgccag accggccgcg cgtcccgttc acgagctcca
     1921 tgcggttccg ctgccgcgag gtcagccgca cgtcgcccgt cacgtggtcg cgcgtgttga
     1981 ccgtcggcac cttgtaccag tcgtactcct tgtggaaggc gttcgcgtcc tcgtagatgt
     2041 tgatgccctc cacgttccgg aaatcgagcg ggtcgggcga gaaggcccac gagccgccgt
     2101 agaagtcggg gtagaagacc tggagcgcga acgactccca gccgccggtg gagccgcccg
     2161 tgagcacgcg ggcccatccc tctggaatcg cgcggaaccg ccgctcgaac tcggggatga
     2221 gctcccggtg gatggcgtcg ccgtacgggc cgttgttcac cgagttcacg gcgtacgaat
     2281 cgtcgaaata cggggtcgcg tgctggatgg tgaccgcgat gaagcgcgga aagccttccc
     2341 cggtccacgc cgcgtgcacg tccgagccct cccggaagcc gagcggaggc cgaagggaga
     2401 agtgcccctg ctggtagagg gtggggtaat gcgcgtccgg atggtccccg tagccgtgcg
     2461 gcaggaggac cgtcatgccc agccagaggt cccgtcccca gaacgtggac aggatttcgg
     2521 agcggatccg gaatcgcttc acccactccg tgtccgccgg gacgtcgacc gcggggagca
     2581 cgcggtcggc cgacagcgcg accgtgccgg acgcccccgg accgacgcgc accgcgaccg
     2641 gctcgctcac caggttgccg ggagagcgcc gccagtcctg gccctcccac cggtcgtcgt
     2701 gcatccagac gacgtggccg tccgaccggc ggaactcggt gtaccggttg acgatcgcct
     2761 ggaccacgta gtcgccctcc ggcagctcgc cgagcgattc gggcgggtag ccagcgaccg
     2821 tggcgtcgaa gacgacggcc tttccgggcg cgagggcctc gacgtcgatg ccgaagaagg
     2881 gcaccccgta ccgcccgacc tggagccgcg gctccggatc gtcgtccgcg gcgaacgcca
     2941 ggaagacgcg ccccgtgatc ggggccgcct cgacggaggc ggggaatgag acctcgaagc
     3001 ggagggcgct gtcctgggcg agcgcgtccg gggagggcgc gagcaccgga agggcggcgg
     3061 cgagggcgaa aaggagtcgc atggggcagg aggcaggtga tttcgaaggg tagtgcgtgt
     3121 ctcgaagcgg ggcaaggctc ctcccgtcgt gtgaagcccg gatgcatttc ccgtcaatcc
     3181 ggcgccggac gcccacaggc attgtccggt gcagggatac cccctacgtt ctgtgtgcca
     3241 tttacgcccg ggatccccgc atgacccaag caattgccct tcccctgccg ctcatcatcc
     3301 agggcgggat gggcgtcggg gtatccgact ggcggctggc ccgtgccgtg tccctcctgg
     3361 ggcagctcgg cgtcgtgtcg ggcaccgcga tcgactcggt cgtggcccgc cgactgcagg
     3421 acggggatct gggcggtcac gtccgccgcg cactcgacgc gttccccttc cgcgaggttg
     3481 ccgaggaggc catgcggagg ttctttcggc cttcgggccg cgacgagggg acgtcgtaca
     3541 ccggcgtgcc catgttccgc atcggtgcca accggttccg ggatctcttc accgtccttg
     3601 ccaacttcgt cgaggtccac ctggcgaagg aggggcacga cggatcggtg ggcgtcaact
     3661 atctcacgaa gatccagatg cccaccctgc cgtccctgta cggggccatg ctggcgggtg
     3721 tggacttcgt gctgatgggc gccggcattc cgcgcgagat tccgggcgcg ctcgacgcgc
     3781 tgtcgcgcca cgagccggcg cacctgaagc tggacatcga cgggaaggcc gatcccgagg
     3841 ccgatgtgct ctcgttcgat cccgcggccg tgggactcac ggggaccgag ccgctgaagc
     3901 gcccccgctt cctgcccatc atcgcgtcga attccctcgc caccatgatg ctccgccgcg
     3961 ccaacgggca ggtggacggc ttcatcatcg aggggcccac ggccggcggg cacaacgccc
     4021 cgccgcgtgg caagaaggtg tacaacgaga agggcgagcc gctctacggc gagcgcgacg
     4081 tcgtgaatct cgaggaaatc gccgagctcg ggctcccgtt ctgggtggcc ggcggcaccg
     4141 gccgccccgg cgcgctcgaa gaggtccgga gccggggagc gacgggcatc caggtcggaa
     4201 cgctcttcgc cttctgcgac gagtcgggcc tgaccgagga gtacaggcgc gaggtggtcc
     4261 gcgaggcgct acggcactcc gtggaggtca ggacggacga tcgggcctcg ccgaccggct
     4321 ttccgttcaa gacggtgggc atggccagga gcctgtcgga cgacgcggtc tacgccgccc
     4381 gcacgcgcat ctgcgacctg ggctacctgc gcgtcccggt gcggctggac gacggcaagg
     4441 tcgaataccg ctgtcccgcc gagcccgagg ccgactggga gcgcaaggga ggagacgcag
     4501 aggtcaccga agggcgacgc tgcctttgca acgcgctcat ggcgaacatc ggcctggggc
     4561 agcagcgctc ggacgggacg tccgagccgc cgctgctcac gagcggagac cagctcgccg
     4621 agctgggccg gttcctcgac gggcgggagc gctactcggc ctccgacgtc ctggactacc
     4681 tcctgggctg acgcccggat cagtcggcgc cgaaccggac ggtcacgccc gcgtggtcgg
     4741 acgggtagta gcccgcgtcg gcgatcacgc ccgtacccgt gatgacggcg gcctccgggg
     4801 tcagccgggt cgcggggccg ttccggtaga gcaccaggtc gtaccgcgtg tcgagctcac
     4861 gcccgggaag caggttgggt gcgaagcagc acgtgaggcc cggattcgcg ccgtggacgt
     4921 cgacccacgc gtccgagaat ccgccgccgg cgaagagccc gtaggtcgag tcatccggcc
     4981 ccgtgttcag gtcccccacg aggatcgtcg tctcgtcgcc cagcacgccg agcagttcgt
     5041 ttgcctgcgc cgcctggatg agccggctct gcgatgccgg agcgggctcg aggtgcgtgc
     5101 tcacgaaacg gaattcgtgg tccccgacgg ccgcgtcgaa ggcgacgtat ccccgctcca
     5161 cccggaacag caggccgccc aggtccacct cgagcgcaac ctcgtagttc gcggtctcga
     5221 cgtcggtcac ctccacggag gacttgacca gcacggcgtc gaaatcggtc caccggatgt
     5281 cccagaagcc gagcggatcc gtgcttacga ccatggggac ctcggcatcg gtgtcctgga
     5341 ccacgccggc cacctcgtag tcgagcccgc ggtcctcgag ggcgtcgagc aggatctgga
     5401 ggtagtcgta cgcgatttcc gtggcgggta ccatcccgcc gaacgccatg tcgcccggat
     5461 cctggacgcg cagcgtggtg atctcctgga gcccgacgat gtggggattc cgggcgtcga
     5521 tctcggcggc gagggcttcc gcgcggacgg caaagtcgtt cgagacgatc tcctgccagg
     5581 tcagaagcac gttcagcggg acgtcttcct ggctcgcgga cgccgggatc cggtcgatgt
     5641 cgccaccgac cgagacgttc cgggtcatga cccggacctt gtccgagtcg tcatcatcga
     5701 acagggagca cgccggcagc ggcgcaaggc acgcgacgag gagtgcgaat gtcagggtgt
     5761 ccttcatgtc ggtcacggtg ttcgggtttc gcccggcgtg caggatcgtc gatcgtgaca
     5821 agcgggacga tactgcacgc cgggccggct gtcgacgcac gggatcagtc ggcgccgaac
     5881 gcgatgacga cgcccgcgtg gtcggacggg tagatgccga ctccctcgat cggatcacgg
     5941 ccggtgatga cgccgcgcgc gggtgcgagc tgcgtcgtgc cgccgttgcg atagaggacc
     6001 aggtcgtagc gcaggtcggg cacgcggccc ggcacgaggt cgggcgcgaa gcagcacgtg
     6061 aggccggggg cctcgccggg cagcaccgcc caggagtcca ggagtccgct cgacgtgagc
     6121 agctcgtagg ccgggtcgcc cggggccgtg ttcaggtcgc cgacgacgat ggccgtgccc
     6181 tcgcccgcga ccgccacgat ctcgagcgcc tggcccagct gcagcggctg caggtccggg
     6241 gccgcgacgg gctccaggtg ggtcgacacg aagcggaagg acgcctcgcc cacctgggcg
     6301 tcgaatgcga cgtagccgcg cttcacggtc aggcctccgg ggccgaacgg cagggaccag
     6361 tcgtacgcga agttctgcgc gagcacgttc tcgatcccga cgtcggccct cgcgaggacc
     6421 gcgtcgtaat cgatcatgcg cacgtcggcg aacgtcggat cggcgctcgt gaccatcggc
     6481 atctcggcct ccgtgtcggc gacgacgccg acgaccacgt agtcgagacc ccgggcctcg
     6541 agggcatcct gcaggatctg aaggaagtcg tacacgacgt ccgtggccgg ctccgcgccg
     6601 cccatgacca tgtcgccggg atcctggatc atgagggtcg tgatctcctg cagcccgatg
     6661 atgtcgggcc cgtgggcctt gacttcctgg gccagggctt ccgcccgcac gtggaagtcg
     6721 ttcgtgacga tctcctgcca ggtctgcgcg acgagcaggg gcacctcgtc catcgacggc
     6781 gcggcgagca cgcggtcgat gtcgccgccg atcgccacgt tccgggtcat gacccggacc
     6841 tcgccgggcc tcttggcgcc gatttcgagg tcgctccgtt ccacgaccgc atcctggccg
     6901 cccagcgtgt cacaggcgaa gacggagagc agacaggcaa ggacgagtag catcatggga
     6961 ttgggtttca tggcattcac cggatcggtg ggatgtgctc gatccgatga tcgccagcat
     7021 ccgacacgac gaaccggaca accatcccgg tcaatccggc catccgcccc ggctcaggcc
     7081 gccgcttcgg ccccacggcg ctcgcactcc gcccgcaggg ccacgatgcc ctggtcgagc
     7141 gcctcggcca gcctgcgctc caggcgcctg cggaggagga cggcaaggat gccctcgaac
     7201 gtctcgccgg tccagacgag cgtgccggcc cggtccgccg agatcctcca cacgtgcacg
     7261 gcgcggatcc ccgccgcctc gccgctccag gcgatgatcc gcctgggctc caggacccgg
     7321 atcgtcgagc ggatcgacgc gaggcccgac ttccaccgga aggtcgttcc gacggcgagc
     7381 ggcccctcca gcttgatcga ccggacatcc ggattccagt cgggccagcg ctcgaggtcg
     7441 gtcagcacct cccagacgat cgagggcggt gcggcgatgc gggtctgcgt ctcggcgcgg
     7501 acgggggtgt tctgcaggag ttccatggcg aagacgtgca taggacgggc ggcgcccgcc
     7561 gccacagcgt
//
LOCUS       DBNHQZ010000160         7554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2213756, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000160 DBNHQZ010000000
VERSION     DBNHQZ010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2213756"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC16YU_04140"
     CDS             <1..240
                     /locus_tag="AC16YU_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6064445.1"
                     /translation="RARSLGYGVEDGAHRAGHLFGIRAPAGVDLDGLRAALERRRISV
                     SVRGDAIRVAPHVYNGADDIDALVATLEDVAGGHP"
     gene            258..884
                     /locus_tag="AC16YU_04145"
     CDS             258..884
                     /locus_tag="AC16YU_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR6064446.1"
                     /translation="MPRLLSIFWLCLLAVAPGASAQDGEALLARLRSRFESVRTFQAQ
                     FSQRTLSTIFEDGQETIRGTLYVRGEAYRLESGTRTLVNDGTTLWIYDAAESQVLISD
                     NVEDEYTFSITGFLYRFDERWTFDSVEKADDAWVLRLSPKNPEDFFRSVAVTMRESDG
                     TVTRVRVVDANDVTMIFTLDDLVENPPLADTVFRFDAPDGVEVVDLRS"
     gene            893..1942
                     /locus_tag="AC16YU_04150"
     CDS             893..1942
                     /locus_tag="AC16YU_04150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6064447.1"
                     /translation="MAASHRNLLFRIGSFALAGGLLYLALRGVDFDQVGADLRAASWG
                     WLVPMAVVTLVSHWLRAWRWTLLLDALPDDDGSRKPIPTRGAFDALMIGYMVNYAAPR
                     LGEVVRTANVASRYGLRFSATLGTVVVERILDVVLLALALLTVPLVFAGRLEPLSAEL
                     IRSASLWTDRVSLGALALVALAVGAMGFVAYRRLVLAGPDGRAGRIRRTLASFAGGVR
                     GLVRTRRRGVLVVTTLAMWMCYAVMAHLPFMMFGFDAAYGITILDSWGLMLLGAIGVA
                     LPSPGGVGSYHYITIQSLVLLFAMPQAQAATYALVTHAGQLLLYVSLGFVLLLAQGGG
                     LAAVLRTDAGTPEQT"
     gene            1917..4358
                     /locus_tag="AC16YU_04155"
     CDS             1917..4358
                     /locus_tag="AC16YU_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR6064448.1"
                     /translation="MRAPRNRHSTFRLAQRLAHRLAATLAAAVLAATSASAQGADPTR
                     APIRVPRELPYDLVHVDADVRILRGGAWDVSLRALAVASTDGPDSLSLDAPGLRIDSL
                     EVVRLVPADSALALPPPGAGSVRALLPPGGVSAGDSVLIRVRYHVPVGHALRSSGSPC
                     CTWTRSPRTGGAAWLPFPHDAPDRFTARLHFHGAPVVAGGRAAPGTGGATVVEYPSPV
                     AADHLIVVAGEPGRTETAGPFAYHAPADRAARVPAFAGTFEAAWAHIERATGHRPAMD
                     TVRVAFVDVGPGAEEAGWGLALADPSLLADPRSGTDPTFRTALLAARFWAGVVLRPAA
                     WTDLWLIEALAHHLALDFVREERGEGEAWGAQVALGEAYLAEASRYRRPLVWDRFAHP
                     ADLADAHASGRGTWFLRQLQGMVGEDAFSRALAVLFARPGEAYTEDLQALLEHSAGRA
                     LAEHFDAWVGGAGHPSIRLDARHDPAREMLTVRLRQAQDGPLVPAVFPVESRLAWRSL
                     AGDGAVSVRLVEPEAEIAIPLPLGPRFVELDPDGRLLAEVDAEHDLSARTAILRYGPI
                     PARYRAALALADEADDPALPLALRMAYEEEAEPVVRRAIVRAAMPVAPSASLESLARL
                     AYGDPDAGVRALALRLLGGFGGREDLAAFVAGAASRDTIQSVQAAAVEALASMGAADA
                     DYFARAALITGSDGDVIRAAGFRALAALPASGSDLRAEVLQHSGANLPVSRILAALEV
                     APRLERSQAVGRRLLELLRHPHYGVREAAAAAAARHLDARTIETRLDEEPDAGVRAAL
                     GAALRAARAGAGRSG"
     gene            4553..4816
                     /locus_tag="AC16YU_04160"
     CDS             4553..4816
                     /locus_tag="AC16YU_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064449.1"
                     /translation="MQAVADRDIRNPLAGLVSDDVYEVLEAHSLLSEKGVRDYQIRKQ
                     FRAMREMDVPAYDAIEQLREAYPYLQFDTIRKIVYKLNGKRHQ"
     gene            complement(4907..5686)
                     /gene="rlmB"
                     /locus_tag="AC16YU_04165"
     CDS             complement(4907..5686)
                     /gene="rlmB"
                     /locus_tag="AC16YU_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR6064450.1"
                     /translation="MSKSASDPDQRYLAGRNPVREALERSESVEKVLLSAAVTGRLAA
                     EIREQCRDAGVPLQTVPPAKLERLVPGVNHQGIVAVVAAVGYADFDEMLRSVAADHET
                     VAREKPVIVLLDGIQDPHNLGAILRSAVAAGARGVVIPERGAAPLNAAALKTSAGTAR
                     RIPVSRVGNLREALLQLKERGYWIAGADGEGDVSVWEMDWDRPIGLVIGSEDRGMRQG
                     VADQCDYRVSIPMAGPAESLNASVAAGILLFAAARARTGRP"
     gene            complement(5689..7086)
                     /locus_tag="AC16YU_04170"
     CDS             complement(5689..7086)
                     /locus_tag="AC16YU_04170"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6064451.1"
                     /translation="MDPGFLIDRAVAEIRAWEATWGPYDHHPSVSVSADRAAEALLAY
                     TDRLKDNYPFFHPRYAGQMLKPPHPAAVAAYVAAMLINPNNHALDGGPATAAMEMEVI
                     RQLRDLFGLPEPGLGHLTSSGTIANLEALWVARELRPGKVVAFSAEAHYTHGRMAGVL
                     GVETRTVPTDPAGHMDLEALERALQDGQVGTVVATAGTTGIGAVDPVADILPIVERHG
                     VRLHVDAAYGGFFRLLAGLDTPLLGAGTRRHLDAISGADSVVVDPHKHGLQPYGCGAV
                     LFRDPRVGRFYRHDSPYTYFTSADLHLGEISLECSRAGASAGALWLTLRLLPLRPDEG
                     LGPVLAACVRAAQDWHRRLVDSTELDPLCAPELDIVAFHPRVDPPRAAAVDQASRALF
                     RAAMEDGDDPVFLSLYRVPATRISVAGHPVRRDRADVHVLRSVLMKPEHELYVPRLHR
                     RVEALAASLRHQVAD"
     gene            complement(7090..>7554)
                     /locus_tag="AC16YU_04175"
     CDS             complement(7090..>7554)
                     /locus_tag="AC16YU_04175"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6064452.1"
                     /translation="YGVGRIGCHLSGDGDWGIPADLSAQPSWFPDWLWAETYPNNILG
                     VDLSAHPVYPTPLYEFAAAVLLFAVLMALRNHPFRAGWLISLYLVLNGLERFFIEKIR
                     VNNVMHLFGIESTQAELIAVLLIVLGLYGMFRLRRRREPDGPVPGPPEPEGD"
BASE COUNT          970 a         2803 c         2676 g         1105 t
ORIGIN      
        1 cgcgcgcgct ctctcgggta cggggtggag gacggcgctc accgcgccgg gcacctgttc
       61 ggcatccgcg ccccggccgg cgtcgacctg gacgggctcc gggcggcgct cgagcggagg
      121 cggatcagcg tgtccgttcg gggcgatgcg atccgagtcg ctcctcacgt gtataacggc
      181 gccgacgaca tcgacgccct ggtcgcgacg ctcgaggacg tcgccggcgg acacccctga
      241 tccggccccg gactgccatg ccacgcctcc tttcgatctt ctggctctgc ctcctcgccg
      301 tcgcccccgg ggcctcggcg caggacggcg aggccctgct cgcgcgcctg cgatcgcgct
      361 tcgagtccgt ccggaccttc caggcccagt tctcccagcg gaccctgtcc accattttcg
      421 aggacgggca ggagaccatc cggggcacgc tctacgtgcg cggcgaggcg taccggctcg
      481 agtccggcac ccggacgctg gtcaacgacg ggaccacgct ctggatctac gacgccgccg
      541 agtcccaggt gctcatcagc gacaacgtcg aggacgagta caccttttcc atcacgggat
      601 tcctgtaccg gttcgacgag cgctggacgt tcgattcggt cgagaaggcg gacgacgcct
      661 gggtgctccg cctctccccg aagaacccgg aggacttttt ccgctccgtc gcggtcacga
      721 tgagggaatc cgacggcacg gtgacgcggg tgcgcgtcgt ggacgccaac gacgtcacca
      781 tgatcttcac gctcgacgac ctggtcgaga atccgccgct cgcggacacg gtgttccggt
      841 tcgacgcccc ggacggcgtc gaggtcgtgg acctgcgcag ctgatcgtcc gcatggccgc
      901 gtcccaccgc aacctgctgt tccggatcgg gagcttcgcc ctcgcgggcg gcctgctgta
      961 cctggccctc cggggcgtcg acttcgacca ggtgggcgcg gacctgcgcg ccgcctcgtg
     1021 gggctggctc gtgccgatgg ccgtcgtcac gctggtgagc cactggctcc gggcctggcg
     1081 gtggacgctc ctgctcgacg cgctccccga cgacgacggc tcccggaagc ccatccccac
     1141 ccggggcgcc ttcgacgcgc tgatgatcgg atacatggtc aactacgcgg ccccgcggct
     1201 gggcgaggtg gtgcgcacgg cgaacgtcgc ctcgcgctac gggctgcgat tcagcgccac
     1261 gctggggact gtggtcgtgg agcggatcct ggacgtggtc ctgctggcgc tcgcgctcct
     1321 cacggtcccg ctggtgttcg ccggccggct cgagccgctt tcggcggagc tcatccggtc
     1381 cgcctccctg tggacggacc gcgtctcgct cggcgccctg gccctggtcg cgctcgccgt
     1441 gggggccatg ggtttcgtcg cctaccgccg cctggtcctc gcgggccccg acgggcgtgc
     1501 ggggcgcatc cggcggacgc tggcgtcgtt cgccggcggc gtccggggcc tcgtccgcac
     1561 gcggcgccgc ggagtgctgg tcgtcacgac gctggccatg tggatgtgct acgcggtcat
     1621 ggcccacctg ccgttcatga tgttcggttt cgatgcggcg tacggcatca cgatcctcga
     1681 ctcgtggggc ctgatgctgc tcggcgcgat cggcgtggcc ctgccgtcac cgggcggcgt
     1741 gggttcgtat cattacatca cgatccagtc cctcgtcctc ctcttcgcca tgccgcaggc
     1801 ccaggcggcg acctatgccc tggtcacgca tgccgggcag ctgctcctgt acgtgagcct
     1861 ggggttcgtg ctgctgctgg cgcagggagg cggactggct gccgtgctcc gaacggatgc
     1921 gggcaccccg gaacagacat agcaccttcc gcctggcgca gcgcctggcc caccgcctgg
     1981 ccgccacgct cgcggccgcg gtgctcgccg cgacgtccgc ctccgcgcag ggggccgatc
     2041 cgacccgggc gccgatccgc gtgccgcgcg agctcccgta cgacctggtg catgtcgacg
     2101 ccgacgtccg catcctccgg ggcggcgcgt gggacgtctc gctgcgggcg ctggccgtcg
     2161 catccacgga cgggccggac tcgctgagcc tcgacgcccc gggcctcagg atagactccc
     2221 tcgaggtcgt ccgtctcgta ccggccgatt cggccctggc cctcccgccc cccggcgccg
     2281 ggtcggtccg tgccctcctg ccccccggcg gcgtttcggc cggcgactcg gtcctgatcc
     2341 gggtccgcta ccacgttccg gtggggcatg cgctgcggtc gagcgggagc ccgtgctgca
     2401 cgtggacgcg gagccccagg acgggcggcg ccgcgtggct tccgttcccg cacgatgccc
     2461 cggaccggtt caccgctcgg ctgcacttcc acggtgcccc ggtcgtcgcc ggcggccgcg
     2521 ccgcccccgg gacgggcggg gccaccgtcg tcgagtaccc gtccccggtc gcggccgacc
     2581 acctgattgt cgtcgccggg gagccggggc ggaccgaaac ggcgggcccc ttcgcctatc
     2641 acgccccggc cgaccgggcg gcccgggtgc ccgctttcgc cgggactttc gaggcggcct
     2701 gggcccacat cgagcgcgcg acgggccacc ggccggcgat ggacacggtg cgggtcgcct
     2761 tcgtggacgt gggcccgggc gcggaggagg ccggatgggg actcgccctc gccgatccgt
     2821 ccctgctcgc ggatccccgc tcggggaccg atccgacgtt ccgcaccgcg ctcctcgcgg
     2881 cccggttctg ggcgggggtc gtgctcaggc ccgcggcgtg gaccgacctc tggctcatcg
     2941 aggcgctggc gcaccacctc gccctggact tcgtccgtga ggagcggggc gagggcgagg
     3001 cgtggggtgc ccaggtggcg ctcggcgagg cctacctcgc ggaggcgtcg cgttaccggc
     3061 gacccctcgt atgggaccgt ttcgcgcacc cggccgacct cgccgacgcc catgcgtcgg
     3121 ggcgcggcac gtggttcctg cggcagctgc agggcatggt cggcgaggac gccttctctc
     3181 gggcgctggc ggtgctgttc gcccgacccg gcgaggcgta caccgaggac ctgcaggcgc
     3241 tgctcgaaca ctcggccgga cgggctctcg cggagcattt cgacgcatgg gtcggcggcg
     3301 ccggccatcc ctcgatccgc ctggatgccc ggcacgatcc ggcgcgggag atgctgaccg
     3361 tccggctccg ccaggcgcag gacggtcccc tcgtgcccgc ggtctttccc gtcgagagcc
     3421 ggctcgcctg gcgctcgctc gcaggggacg gcgcggtgtc cgtccgcctc gtggagccgg
     3481 aagccgagat tgcgatcccg cttcccctcg gcccgcgatt cgtggaactg gaccccgacg
     3541 gccgcctgct ggccgaagtg gatgcggagc atgacctgag cgcccggacg gccatcctgc
     3601 gatacgggcc catccccgcg cgctaccggg ccgccctggc actggcggac gaggcggacg
     3661 atccggcgct cccgctggcg ctccggatgg cgtacgaaga ggaggcggag ccggtggtcc
     3721 gccgggccat cgtgcgcgcg gcgatgcccg tcgcgccgtc cgcgtccctg gaatcgctgg
     3781 cccggctcgc gtacggggac ccggatgccg gggtccgcgc actggcgctg cggctcctcg
     3841 gcggattcgg gggccgcgag gacctggccg cgttcgtggc gggcgccgcc tcgcgggaca
     3901 cgatccagtc ggtccaggcc gcggcggtcg aagcgctcgc ctcgatgggc gcggccgacg
     3961 ccgactactt cgcgcgggcg gccctcatca ccgggtcgga cggcgacgtg atccgggccg
     4021 cgggcttccg ggccctcgcc gccctgcccg cttccggctc cgaccttcgc gccgaggtgc
     4081 tccagcattc gggcgcgaac ctgccggtga gccggatcct ggccgccctc gaagtcgccc
     4141 ctcgcctgga gcgcagccag gcggtcggcc gtcgtctcct cgagctgctc cggcaccccc
     4201 actacggcgt gcgcgaggcg gcggccgccg ccgccgcccg acacctcgat gcccgcacga
     4261 tcgagacacg cctggacgag gagccggatg ccggcgtgcg cgcggcgctc ggggcggccc
     4321 tgcgggctgc ccgtgcgggc gccggccgct ccgggtgacc gatcgggcgc cggcgccgcg
     4381 cccgcccgta ggctacgatc gacggctcgt cgcaatcgcc cgaccattcg ggtcatcttg
     4441 cttggcaggc ccttcgacct gccctaccct ttacccgcgg acagggcgcc ttttgccccg
     4501 ccaggcgtgg aagggcgcgc gccgctcact accatcaagc atcccaccga ccatgcaagc
     4561 agtggccgac cgggacatca ggaatcccct tgccgggctc gtcagcgatg acgtgtacga
     4621 ggtgctcgaa gcgcacagcc tgctcagcga gaagggcgtc cgggactacc agatccggaa
     4681 gcagtttcgc gcgatgcgcg agatggacgt tcccgcgtac gacgccatcg agcaactcag
     4741 ggaagcctat ccctacctcc agttcgacac gattcggaag atcgtctaca agctcaacgg
     4801 caagcggcac cagtagaccg ccacgtcggg ccgtttaccc ggtatcgacg gcagcccgcg
     4861 gtgggctcca ccgggcacgc ggcgccgcgc ggacggcagg gacggcctac ggccgccccg
     4921 ttcgggcacg ggcagcggca aacagcagga ttccggccgc gacagaggca ttgagggact
     4981 ccgcgggccc tgccatgggg atgctgacgc ggtagtcgca ctggtcggcc actccctggc
     5041 gcatcccgcg atcctcgctt ccgatcacga ggccgatggg acggtcccag tccatctccc
     5101 acacggacac gtcgccctcg ccgtccgctc ccgcgatcca gtacccgcgc tccttcagct
     5161 gcaggagggc ctcgcggagg tttccgaccc tcgagacggg tatccgccgg gccgtccccg
     5221 cggacgtctt cagcgccgcg gcgttcagcg gggccgctcc ccgctccgga atgacgaccc
     5281 ccctcgctcc cgcggccacc gcgctgcgca ggatggcccc gaggttgtgc ggatcctgga
     5341 tgccgtccag gagaacgatc acgggcttct cgcgggcgac cgtctcgtgg tccgcagcga
     5401 cggatcgcag catctcgtcg aagtcggcat agcccacggc cgcgaccacg gctacgatcc
     5461 cctggtggtt caccccgggg accagcctct cgagcttcgc aggcggaacg gtctgaagcg
     5521 gcacaccggc gtcccggcac tgctcccgga tctcggccgc caggcgcccg gtcacggccg
     5581 ccgacagcag cactttctcg acgctctccg agcgttcgag cgcctcccgg accgggttcc
     5641 ggcccgcgag gtagcgctga tcgggatcgg aagcggactt cgacatgttc agtccgccac
     5701 ctggtggcgc agcgacgcgg cgagcgcctc cacccgccgg tgcagccgcg gcacgtagag
     5761 ctcgtgctcg ggcttcatca gcacgctccg gagcacgtgg acgtcggccc ggtcacggcg
     5821 gacggggtgt cccgcgacgg aaatccgcgt cgcgggcacg cggtacaggc tcaggaaaac
     5881 ggggtcgtcc ccgtcctcca tcgccgccct gaacagggcc cgggatgcct ggtccacggc
     5941 cgcggccctc ggcgggtcca cccggggatg gaacgccacg atgtccagct ccggcgcgca
     6001 cagggggtcg agctccgtcg aatcgacgag cctccggtgc cagtcctgcg ccgcgcgcac
     6061 gcacgccgcc aggacggggc cgagtccttc gtccggccgc agcggcagca gccgcaaggt
     6121 gagccagagc gccccggccg aggcgcccgc gcgcgagcat tcgaggctga tctcgcccag
     6181 gtgcaggtcc gcggacgtga aatacgtgta cggggagtcg tgccggtaga agcggcccac
     6241 cctggggtcg cggaagagca ccgcgccgca gccgtagggc tgcaggccgt gcttgtgcgg
     6301 gtcgacgacc accgaatccg cgccggaaat cgcgtcgagg tgccgccggg tgccggcccc
     6361 cagcagcggg gtgtccaggc cggcgaggag gcggaagaag ccgccgtacg cggcgtccac
     6421 gtgcaggcgc acgccgtgac gctcgacgat aggcaggatg tccgccaccg gatccacggc
     6481 gccgatgccg gtggtcccgg cggtggccac caccgtcccg acctggccat cctggagcgc
     6541 ccgctccagg gcctcgaggt ccatgtgccc ggccggatcc gtcggcacgg tacgcgtctc
     6601 gacgcccagg accccggcca tgcgcccgtg cgtgtagtgc gcctcggcgg agaaggccac
     6661 gaccttcccc ggtcgaagct cccgcgccac ccacagggcc tccaggttgg cgatcgtccc
     6721 gctcgaggtg aggtggccga ggcccggttc cggaagcccg aacaggtccc ggagctgccg
     6781 gatgacctcc atttccatgg cggcggtcgc ggggccaccg tccagggcgt ggttgttcgg
     6841 gttgatgagc atcgccgcga cgtaggcggc cacggccgcc gggtggggcg gcttcagcat
     6901 ctggcccgcg taccggggat ggaagaacgg gtagttgtcc ttgagccggt ccgtgtacgc
     6961 gagcagggcc tcggccgcgc ggtccgccga gaccgagacc gacgggtgat ggtcgtacgg
     7021 tccccatgtc gcctcccatg cccggatctc ggcgacggcg cgatctatca ggaaacccgg
     7081 atccatggct cagtcaccct ccggctcggg cggacccggc acgggcccat ccggctcgcg
     7141 gcgccggcgc agccggaaca tcccgtacag gccgagcaca atgaggagca ccgcgatcag
     7201 ctcggcctgc gtggactcga tgccgaacag gtgcatcacg ttgttcacgc ggatcttctc
     7261 gatgaagaac cgctccaggc cgttcagcac caggtacagg gaaatgagcc acccggcgcg
     7321 gaacgggtgg ttccggagcg ccatgaggac cgcgaacagc aggacggcgg ccgcgaactc
     7381 gtagagcggc gtcggataca ccgggtgcgc cgacaggtcc acgcccagga tgttgttcgg
     7441 gtaggtttcg gcccagagcc agtccgggaa ccacgacggc tgggccgaga ggtcggccgg
     7501 aatgccccag tccccgtcgc ccgacaggtg gcatccgatc cggcccaccc cgta
//
LOCUS       DBNHQZ010000161         8631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2350890, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000161 DBNHQZ010000000
VERSION     DBNHQZ010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2350890"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16YU_04180"
     CDS             <1..283
                     /locus_tag="AC16YU_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064453.1"
                     /translation="VFLDEPTGGVDPVTRRQFWEMIYDAAGGGTTVFVTTHYMDEAEY
                     CDRVSIMVDGRIEAIGTPRQLKERYGVESVDEVFVRLARGGASGPEGAA"
     gene            280..1383
                     /locus_tag="AC16YU_04185"
     CDS             280..1383
                     /locus_tag="AC16YU_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064454.1"
                     /translation="MTTFLGFVVKEFRHIFRDRRTLVILFGMPIAQLVLFGFAIRNEI
                     LDVKVAVVDLSRDQVTRELVDRFEASSQFEVVELGSTADIEDRFRRGKIKQAAVFEPG
                     FARRLEREGLATLQLITDATDPNTANTILAYATAIVADYQAEIARGDAAGVRIVTDTR
                     MRFNPELRSVVLFVPGLVALILMLVCALMTSITLTREKETGTREILLVSPLRPVQIIV
                     GKVLPYLALSLVNVFTILLLANLVFSVPVRGSILLLVAECLLFTICALSLGILISART
                     SSQQVAMMIALAGLLMPTVLLSGFIFPVSSMPTVLQWVSHIVPAKWFLVIIRGVMIKG
                     VGLETVWLETMVLVGMTAFFLGVGARNFNERLG"
     gene            1391..2512
                     /locus_tag="AC16YU_04190"
     CDS             1391..2512
                     /locus_tag="AC16YU_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064455.1"
                     /translation="MRIVLILMRKEFLQIFRNRAMLPILFVMPIIQLLVLAYAVTYEI
                     RNVPISVVDADRSTVARDLVQKLEASGTFFVASRPPDLRSAGEDLLRGRTEMILGIPR
                     GMERDLHTVGAARIQMILDAEDGAAAGVIQAYATQIIAAFNREIQVDFRPAAPPASQA
                     RILVAPRHWYNPALDYQTFMVPGILVLLVTMIGTFLSAMNVVREKEIGTIEQLNVTPI
                     RKSEFLVAKLMPFWIIALAELAVGLVVARLLFDIPMLGSLWLLFGLSGVYLVVMLGIG
                     LWISTVTETQQQAMFVAWFIMVIFILMSGLFTPVDSMPRWAQLATRLNPIAYFIEIMR
                     RVMLKGAGLADVQVQLWALVGYAAVMLTLAVRQYRKVTA"
     gene            complement(2526..4310)
                     /locus_tag="AC16YU_04195"
     CDS             complement(2526..4310)
                     /locus_tag="AC16YU_04195"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6064456.1"
                     /translation="MDTRSHAAPASTSDPTYSKLDPLWPPAPPPKGRSAAMLTNVIEH
                     HVDRLYSVLRVLSPIAKLPKGGPVFVTRFRDVQEVLDRPEVFAVIYAPMIDRSVGGFM
                     LAHDGSEYNKDKGLMRSLMNHSDLPIVREMVGRLTDQSIDEAVRYGTLEVVRNVSRRV
                     PILVTGQYFGFPGPDVETMFRWARATQYDMFHNQEMSGRIHDENLEAGREMHAYLKDV
                     LVPQLRRELERNPALDDVVSRLLRMKLPKEIGFDENRVLSNIMGLLVGGVETTSQAIV
                     NILDQLLRRPKVLEGAKQAARDGDDELVGRYCWEALRFNPINPFVMRQCTQDYRVAAG
                     TWRTKKIETGRVVLVGTRSAMMDGREIPKPKQFRLDRPDYHYLHLGYGLHRCLGDHVS
                     MVQVPEIVKRLLLRANLRRAPGPAGQIDMNGGPFPESFSVCFDEARPGGSPTTPSPGS
                     PSSDPSKPVNPVSKPQDEVAETLSHYAKAFLTFDVAKSMPYFNVPLMFISDAGILTFD
                     EPEMVQGFLMKYVQVLQSEHYARVALSKPDFEVLNHSTVVAKFSVTRYDDQDKVIGSL
                     GANYTFCKSGGAWKIAVAVLLAKDPADG"
     gene            complement(4331..6241)
                     /locus_tag="AC16YU_04200"
     CDS             complement(4331..6241)
                     /locus_tag="AC16YU_04200"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HIR6064457.1"
                     /translation="MVNPRKAVAVGLLVVPVALFVLLHAAAWYLGRGIVLPETEDVEV
                     VTLDQNWTAADRQWFYHTSQGGAFALLLRYPWLMAMEQPRLPWSIVGPVPRFMADDYI
                     TRFGFLPNPRERYSADSITFGWAIDAHSPAYAGTHNNPDRLPVGFARTPEFTDPDDGK
                     RYDVIGFTCAACHTGQLNYRGKGVRIEGGPALTNLTKFEQAVGLSLALTYLIPQRFDR
                     FARAVLGEDHTRTERSELRGQLSLLIKKGEDYEALVKGPPAIYPTEEGFGRLDALGRI
                     GNFVFGMEVDEANMVVADGPVNYPHIWDTPWFDWVQYNGSIKQPIIRNAGESMGVFAD
                     VNFDPADTTRLFDSTIMPGNLYEMESLIRGPAPFEGLKPPEWPEEIFGAIDRDLAARG
                     RDLYATHCEGCHHPVRSETPPYLPDEYWTEVDSFGNRYLDLKLVNLYEIGTDPRAAVN
                     FAQRVVNLGELGEKFREEPGRGGLRSAGDALPFLVSRTVDRKFDQLGLPDSLRDLWRG
                     LRPNDIRAPLAYMARPLNGVWASPPFLHNGAVPTIYQLLSPHAERDAKFWLGSKEYDP
                     VNVGYTTRQVRGGFQLDTSLPGNSNRGHQFEGDFDPTAPGFNWNALPSGIIGPGLSPD
                     ERMALLEYLKSI"
     gene            6550..>8631
                     /locus_tag="AC16YU_04205"
     CDS             6550..>8631
                     /locus_tag="AC16YU_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6064458.1"
                     /translation="MASEERGDPFFDEVFPVELQEIDARRRFHGLEETGGAGPPSAGL
                     GLTGLSLSGGGIRSASFSLGVVQRIARAGLLRHVDFLSTVSGGGYIGACLSSLLSDPE
                     RNPDAPFPLDRVAGRPEPPALQHIRNGSKFLTPGRLLERVRLPVMIARGTVLSFLVFL
                     PLVMAAVAITEVLYEMGPSPRVLLASIPVEAWFLLLLIVTVPFLGRLVGRRFDWDFRN
                     RYQLLLAVFALLLVATIATVPLAHLVQAVVEVPWDDTREALEHGRLLEALGVPGYWGW
                     LAPLLVLALFGLLARASVLSRRLADRVVLYAAGVLGPAIIGLVYLVLCVHQIDSPFLS
                     PGFRDDLGRMAAQPEAVHETPDALRSAFRNRGMELGPNVRVTLEPRPGSGQAGDSTWV
                     VRSAGGGSTPGVFRIAPASPGRLRIEGMKARLFHPPEDLYFIILFVLLVLFNVFLMDV
                     NTASLHGYYRDRLSRAFLFRVDPTTGALQRNDTQKLSELGPGAPYHLVNATMNLQGSH
                     DHSLRGRMADFFLFSKRFVGSARTGYARTEALEDVDTHLDLAAATAISGAAASPNMGS
                     VTRRTLTFIMTLLNLRLGYWLVNPRVVESAGYMGRLLSRAAGPGLLLREAFGDLNERG
                     RFVNLSDGGHIENLGLYQLLKRRCRFIIAVDAGADPASLFGDLAQLIRFAGIDLGVTI
                     DLDTQALGADAH"
BASE COUNT         1258 a         2909 c         2966 g         1498 t
ORIGIN      
        1 ggtcttcctg gacgagccga ccggcggggt cgaccccgtc acccggcggc aattctggga
       61 aatgatctac gacgctgccg gcggcggcac cacggtattt gtgacgaccc attacatgga
      121 cgaggccgag tactgcgacc gggtctccat catggtggac ggacggatcg aggcgatcgg
      181 gaccccgcgg cagctcaagg agcgctacgg cgtggagagc gtggacgagg tcttcgtgcg
      241 tctcgcacgc ggaggggctt caggtccgga gggtgcggca tgacgacctt cctcggattc
      301 gtggtcaagg agttccgcca catcttccgt gaccggagga cgctggtcat cctcttcgga
      361 atgcccatcg cccagctggt cctcttcggg ttcgcgatcc gcaacgagat cctggacgtg
      421 aaggtggcgg tcgtggacct gtcgcgggac caggtgacac gcgagctcgt ggaccggttc
      481 gaggcttcgt cccagttcga ggtcgtcgag ctcggctcca cggccgacat cgaggaccgc
      541 ttccgtcgcg gaaagatcaa gcaggcggcc gtcttcgagc ccgggttcgc ccgccgcctg
      601 gagcgggagg gcctcgcaac cctccagctg atcacggacg cgaccgatcc gaacaccgcg
      661 aacacgatcc tggcgtacgc gacggccatc gtggccgact accaggccga gattgcccgg
      721 ggagatgccg cgggcgtccg gatcgtgacg gatacgcgca tgcgcttcaa cccggagctg
      781 cgcagcgtcg tgctgttcgt gccgggcctg gtcgcgctca tcctgatgct ggtgtgcgcg
      841 ctcatgacct cgatcacgct gacgcgcgag aaggagaccg gcaccaggga gatcctgctc
      901 gtgtcgccgc tgcgccccgt gcagatcatc gtgggcaagg tgctgccgta cctcgcgctc
      961 tcgctcgtga acgtgttcac gatcctgctg ctcgccaacc tggtgttctc ggttccggtc
     1021 cgcggcagca tcctgctgct cgtcgccgag tgcctgctct tcacgatctg cgccctctcg
     1081 ctgggcatcc tgatctcggc gcgaacgtct tcgcagcagg tggccatgat gatcgcgctc
     1141 gccggactgc tgatgcccac cgtcctgctc tcgggcttca tcttcccggt gtcgagcatg
     1201 ccgaccgtgc tgcagtgggt gagccacatc gtgccggcga agtggttcct cgtcatcatc
     1261 cggggcgtca tgatcaaggg cgtgggcctc gagaccgtct ggctggagac gatggtgctc
     1321 gtcggcatga ccgccttctt cctcggggtc ggggccagga atttcaacga gcggctcggc
     1381 taggcgaccc atgcgcatcg tcctcatcct catgcggaag gagttcctgc agatcttcag
     1441 gaaccgggcc atgctgccca tcctgttcgt gatgccgatc atccagctgc tcgtgctcgc
     1501 ctacgcggtg acgtacgaga tccggaacgt cccgatcagc gtcgtggacg cggaccggtc
     1561 caccgtggcc cgcgacctgg tccagaagct cgaagcctcc ggcacgttct tcgtcgccag
     1621 ccgtccgccg gacctccgct cggcgggcga ggacctgctg cgcgggcgca cagagatgat
     1681 cctgggcatc ccccggggca tggaacgcga cctgcacacg gtcggagcgg ccaggatcca
     1741 gatgatcctg gacgccgagg acggggccgc ggcgggcgtc atccaggcct atgccacgca
     1801 gatcatcgcc gcgttcaacc gcgagatcca ggtcgacttc cggccggcgg caccccccgc
     1861 gagccaggca cggatcctgg tggcgccgag acactggtac aatccggccc tcgactacca
     1921 gacgttcatg gtgcccggaa tcctggtgct cctcgtcacc atgatcggca cgttcctctc
     1981 cgcgatgaac gtggtccgcg agaaggagat cggcacgatc gagcagctca acgtcacccc
     2041 gatccggaaa tcggagttcc tggtggccaa gctcatgccg ttctggatca tcgccctcgc
     2101 cgaactggcc gtcggcctgg tcgtggcccg cctcctgttc gacatcccga tgctcgggag
     2161 cctttggctg ctcttcggcc tctccggcgt gtacctcgtc gtgatgctcg gcatcgggct
     2221 ctggatatcg accgtcaccg agacccagca gcaggccatg ttcgtcgcct ggttcatcat
     2281 ggtcatcttc atcctgatga gcgggctctt cacgcccgtc gactcgatgc cacgctgggc
     2341 gcagctcgcg acccgcctga acccgatcgc gtacttcatc gagatcatgc gccgggtgat
     2401 gctcaagggc gccggcctcg ccgatgtcca ggtccagctc tgggcgctcg tcggctacgc
     2461 ggccgtgatg ctcacgctcg cggttcggca gtacaggaag gtgacggcct gacgggcggc
     2521 cgggctcatc cgtccgcggg gtccttcgcc aggagcacgg cgaccgcgat cttccacgcg
     2581 ccgcccgact tgcagaaggt gtagttcgcg cccaggctcc cgatgacctt gtcctggtcg
     2641 tcgtaccggg tcacgctgaa cttggcgacg acggtcgagt ggttgagcac ctcgaagtcg
     2701 ggcttcgaca gcgcgacgcg cgcgtagtgc tccgactgca gcacctggac gtacttcatc
     2761 aggaagccct ggaccatctc cggctcgtcg aacgtcagga tgcccgcgtc cgagatgaac
     2821 atgagcggca cgttgaagta cggcatcgac ttcgcaacgt cgaacgtcag gaaggccttg
     2881 gcatagtgcg agagggtctc ggcgacttcg tcctggggct tggacacggg attcaccggt
     2941 ttcgatgggt cggaggatgg ggatccgggg gacggagtgg tcggggagcc ccccggacgc
     3001 gcctcgtcga agcacacgct gaaggactcg gggaacggcc cgccgttcat gtcgatctgt
     3061 ccggccgggc cgggcgcccg gcggaggttc gcccgaagga gcaggcgctt cacgatctcg
     3121 ggcacctgga ccatgctcac gtggtcgccc aggcagcggt ggaggccgta cccgagatgc
     3181 aggtagtggt agtccggccg gtccaggcgg aactgcttcg gcttcgggat ctcgcggccg
     3241 tccatcatgg ccgagcgggt gcccacgagc accacgcgcc cggtttcgat cttcttcgtc
     3301 cgccacgtgc ccgccgccac gcggtagtcc tgcgtgcact gccgcatcac gaaggggttg
     3361 atcgggttga agcggagggc ttcccagcag tagcgcccca cgagctcgtc gtcgccgtcc
     3421 cgggcggcct gcttcgcccc ctcgagcacc ttcgggcgcc gcaggagctg gtccaggatg
     3481 ttgacgatcg cctgggacgt cgtctcgacg ccgccgacga gcaggcccat gatgttcgag
     3541 aggacccggt tctcgtcgaa gccgatctcc ttcgggagct tcatccgcag caggcgcgag
     3601 accacgtcgt ccagggcggg attccgctcg agctcgcgcc ggagctgcgg cacgaggacg
     3661 tccttcaggt acgcgtgcat ctcgcgcccg gcctcgagat tctcgtcgtg gatgcggccg
     3721 gacatctcct ggttgtggaa catgtcgtac tgggtggcgc gcgcccagcg gaacatggtc
     3781 tcgacgtcgg ggccggggaa gccgaagtac tggccggtga cgaggatggg cacgcggcgc
     3841 gagacgttcc gcacgacctc gagcgtcccg taccggaccg cctcgtcgat cgactggtcg
     3901 gtcagacggc cgaccatctc gcggacgatg ggcagatcgg agtggttcat gaggctccgc
     3961 atcagcccct tgtccttgtt gtactccgag ccgtcgtgtg ccagcatgaa cccgccgacg
     4021 ctgcggtcga tcatcggggc gtagatcacc gcgaagacct cgggccggtc cagcacctcc
     4081 tggacgtcgc ggaaccgggt cacgaacacg gggccgccct tcggcagttt cgcgatcggg
     4141 ctcagcacgc gcaggaccga gtacagccgg tccacgtggt gctcgatcac gttcgtgagc
     4201 atggccgccg accggccctt cggcggcggc gccgggggcc agagcggatc cagcttcgaa
     4261 tacgtgggat ccgaggtgga tgccggcgcg gcgtgggaac gggtgtccat ggagctctcg
     4321 gccacgggtt tcagatggat ttgaggtact cgaggagggc catccgctcg tcgggcgaga
     4381 ggccggggcc gatgatgccg ctgggcaggg cgttccagtt gaagccgggc gcggtcgggt
     4441 cgaagtcccc ctcgaactgg tgcccccggt tggaattgcc cggcagggac gtgtcgagct
     4501 ggaatccgcc acggacctgg cgcgtcgtgt accccacgtt caccgggtcg tactccttgc
     4561 tgccgagcca gaacttcgcg tcgcgctcgg catgcgggct caggagttgg tagatcgtcg
     4621 gaacggcgcc gttgtgcagg aagggcggag acgcccatac gccgttcagc ggccgggcca
     4681 tgtaggcgag gggcgcccgg atgtcgttgg gccggagccc gcgccagaga tcgcgaaggg
     4741 agtccggcag cccgagctgg tcgaacttcc ggtcgaccgt ccgcgagacc aggaagggca
     4801 gggcgtcgcc cgccgatcgg agtccgccgc gccccggctc ctcgcggaac ttctcgccga
     4861 gctcgccgag gttcaccacg cgctgcgcga agttgacggc cgccctcgga tcggtgccga
     4921 tctcgtacag gttgacgagc ttcaggtcca ggtagcggtt gccgaacgag tccacctcgg
     4981 tccagtattc gtccgggagg tagggcggcg tctccgagcg gaccggatgg tggcagccct
     5041 cacagtgcgt ggcgtagaga tcccgtccgc gcgccgccag gtcgcggtcg atggcgccga
     5101 agatttcctc cggccattca gggggcttga gcccctcgaa cggggccgga ccgcggatca
     5161 gcgactccat ctcgtagagg ttgcccggca tgatcgtcga gtcgaagagc cgggtcgtgt
     5221 cggccggatc gaagttcacg tcggcgaaca ctcccatgga ctcgccggca ttccggatga
     5281 tgggctgctt gatcgagccg ttgtactgga cccagtcgaa ccagggcgtg tcccagatgt
     5341 gggggtagtt cacggggccg tcggcgacca ccatgttggc ctcgtcgacc tccatgccga
     5401 acacgaagtt gccgatgcgc ccgagcgcgt cgagccgtcc gaaaccctcc tcggtcgggt
     5461 agatcgcggg cggacccttg accagggcct cgtagtcctc gcccttcttg atgaggaggc
     5521 tcagctggcc ccgcagctcg gagcgttcgg ttctcgtgtg gtcctcgccg agcacggccc
     5581 gcgcaaagcg gtcgaaacgc tgcgggatca ggtacgtgag cgcaagcgag aggcccacgg
     5641 cctgttcgaa cttggtgaga ttcgtcagcg ccgggccgcc ctcgattcgg acgccctttc
     5701 cccggtagtt gagctgcccg gtgtggcagg ccgcgcatgt gaagccgatg acgtcgtatc
     5761 gcttgccgtc gtccgggtcc gtgaactccg gcgtgcgcgc gaagccgacg ggcagccggt
     5821 cggggttgtt gtgcgtcccg gcgtaggccg gcgagtgcgc gtcgatcgcc cacccgaacg
     5881 tgatgctgtc cgcggagtag cgctcgcgcg ggttcgggag gaagccgaag cgcgtgatgt
     5941 agtcgtccgc catgaaccgc ggcacggggc ccacgatgga ccacggcagg cgcggctgct
     6001 ccatcgccat gagccaggga taccggagca gcagcgcgaa tgccccgccc tggctcgtgt
     6061 ggtagaacca ctggcggtcc gccgccgtcc agttctggtc gagcgtcacg acctcgacat
     6121 cctcggtctc gggcagcacg atgccccgtc cgaggtacca cgcggccgcg tggagcagca
     6181 cgaacagggc gaccggaacg accagaaggc cgacggcgac cgccttgcgc ggattgacca
     6241 tgttgcggtc gggaggacgc ccgggcacgc ggctgggccc gccggggcgc cgaaatcccg
     6301 gtctgtgatg agtgggggtg acggggcggc ctccaggagg gggtccggag aggcagccgc
     6361 ggcgcaggtt aagacattca cggggatttc gcgaagtgcg cgcttcggcg tccgcgattt
     6421 cgccgcctcc gtgcatcccg ttgtcccgct gacgctttgc cgataccttg tgcgccccga
     6481 agtgctgccc ccggcccgcc cgtctccacg ggacgggctc cgaacccccg aacctgtccc
     6541 cgcaagtcca tggcgtccga agagcgcggc gatcctttct tcgacgaggt cttcccggtc
     6601 gagctgcagg agatcgacgc gaggcgtcgg ttccacggat tggaggagac cggcggcgcc
     6661 ggccccccct ccgccgggct cgggctcacg ggcctctcgc tttcgggagg gggcatccgc
     6721 tcggcctcgt tcagcctggg ggtcgtccaa cggatcgcac gggcgggcct gctccggcac
     6781 gtcgacttcc tctccaccgt ctccgggggc ggctacatcg gggcgtgcct gtcctcgttg
     6841 ctgtcggatc cggagcggaa tccggatgcc ccttttccgc tcgaccgggt ggccgggcga
     6901 cccgagccgc cggccctgca gcatatccgg aacgggtcca agttcctgac acccgggaga
     6961 ctgctcgagc gggtgcgcct tccggtcatg atcgcccggg gcacggtgct gagctttctc
     7021 gtcttcctgc cgctcgtcat ggcggcggta gcgatcaccg aagtcctgta cgagatgggc
     7081 ccgagccccc gcgtactcct ggcctcgatc cccgtggagg cctggttcct cctgctgctc
     7141 atcgtcacgg tgcctttcct cggtcgtctc gtcgggcgcc ggttcgactg ggatttccgc
     7201 aaccgctacc agctgttgct ggcggtcttc gccctcctgc tggtcgcgac cattgcgacg
     7261 gtaccgctcg cgcacctggt ccaggccgtc gtcgaggtgc cgtgggacga cacgcgggag
     7321 gcgcttgagc acggccggct tctggaggcg ctgggcgtgc ccgggtactg gggctggctg
     7381 gcgccgctcc tcgtcctcgc gctcttcggc ctcctggcac gcgcctcggt cctgtcccga
     7441 cgactggccg accgcgtcgt gctctatgcc gccggcgtcc tggggccggc catcatcggg
     7501 ctggtgtacc tggtgctctg cgtccaccag atcgattcac cgttcctctc gccgggcttc
     7561 cgcgacgacc tgggccggat ggcggcgcag cccgaagccg tccacgagac tccggacgcg
     7621 ctccgcagcg cgttccggaa ccggggcatg gagctgggcc cgaacgtgcg cgtgaccctg
     7681 gagccgaggc cgggctcagg gcaggcaggc gatagcacgt gggtcgtgcg gagcgcgggc
     7741 ggcggttcca cgccgggcgt gttccgcatc gcgccggcga gcccgggcag gctccggatc
     7801 gaggggatga aggcgcgcct cttccacccg ccggaggacc tctacttcat catcctgttc
     7861 gtcctgctcg tcctgttcaa cgtcttcctc atggacgtga acacggcctc gctgcacggc
     7921 tactaccggg accgtctgag ccgcgccttc ctgttccggg tcgacccgac gacaggcgcc
     7981 ttacagcgga acgacacgca gaagctctcc gagctcggcc cgggcgcccc gtatcacctg
     8041 gtcaacgcga cgatgaacct gcaggggagc cacgaccaca gccttcgcgg ccggatggcc
     8101 gacttcttcc tgttcagcaa gcgcttcgtg ggcagtgccc ggacgggata cgctcggacc
     8161 gaggccctgg aggacgtgga cacgcacctg gacctggccg cggcgaccgc gatctcgggc
     8221 gcggcggcct cgcccaacat gggctcggtg acgcgccgca ccctgacctt catcatgacc
     8281 ctcctgaacc tgcgcctcgg gtactggctg gtgaatcccc gcgtggtcga gtccgccgga
     8341 tacatggggc gcctgctcag ccgggcggcc ggaccggggc tgctgctgcg ggaggccttc
     8401 ggggatctga acgaacgcgg ccgattcgtg aacctctccg acggcgggca catcgagaac
     8461 ctggggctct accagctgct caagcggcgg tgccgcttca tcatcgcggt cgatgccggg
     8521 gccgatccgg cgagcctgtt cggggacctc gcccagctca tccggttcgc cgggatcgac
     8581 ctgggcgtga cgatcgacct cgacacgcag gccctgggcg cggacgccca c
//
LOCUS       DBNHQZ010000162         6268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1538986, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000162 DBNHQZ010000000
VERSION     DBNHQZ010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1538986"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..455)
                     /locus_tag="AC16YU_04210"
     CDS             complement(57..455)
                     /locus_tag="AC16YU_04210"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6064459.1"
                     /translation="MIEDLEIREGLDGLNPARITTLYRRAPLTRRADAPERVWLMFER
                     SSLVLTAWRQGRLVGIARVLTDGVAVTYVCDVAVEPDVQGLGIGRSLVQGIVDRSRGT
                     RIVVRDDGTSRWYRRLGFRRVEDAWELEGA"
     gene            complement(490..1509)
                     /locus_tag="AC16YU_04215"
     CDS             complement(490..1509)
                     /locus_tag="AC16YU_04215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064460.1"
                     /translation="MKTQPGTGGPRAADVVLKDLVRTFGAVRALDGVSLDIAAGSFVS
                     LLGPSGCGKSTLLRVVAGLEQPDAGSVHIAGRDVTRVAPQARPTALVFQSYALFPSMT
                     VGENVEYGLRVRRVDRRERARRVHETLGRVGLEDLADRPVTQLSGGQQQRVAVARALA
                     VEPAVLLFDEPLSNLDVALRERTRDELRQLQQELGTTAIYVTHDQEEALAVSDRIAVM
                     REGRLVEAGSPEALYERPDTAYVAGFLGGSNLVEGPAAERLAGGPPPDGRTVLAVRPG
                     HLVFAPEGPLAGRVVSRRYLGSFTEWRIRTDAGELRVRTAGFDDRQPDVRLAATRTLW
                     VRGPI"
     gene            complement(1517..2689)
                     /gene="sucC"
                     /locus_tag="AC16YU_04220"
     CDS             complement(1517..2689)
                     /gene="sucC"
                     /locus_tag="AC16YU_04220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR6064461.1"
                     /translation="MKIHEYQAKDILSSYGVAVQPGVVATTVDEAVEAARRVGADTGR
                     DVFVVKAQIHAGGRGKGGGVKLARGIEEVRSRAGDILGMMLKTHQTGPEGQKVRSVLI
                     ADAVDIEKEFYIGITLDRTRSMPVIMASTEGGVEIEKVAAETPEKILKEWIDPSIGLQ
                     GFQARKLAFGLGFTGPAFKQAVGFIGALYRAYEASDCSLAEINPLVLTPAGEVLAIDA
                     KINLDDNALYRHPDLVKLRDTNEEDPLEVEASEHSLNYIKLDGNVGCMVNGAGLAMAT
                     MDIIKLAGGEPANFLDVGGGANPETVEAGFRIILKDPHVKAILINIFGGIVRCDHVAR
                     GVVEAARNVGIDMPLIVRLQGTNAEEGKQILDRSGLEVETAILFQEAADKVTAALA"
     gene            2869..3957
                     /locus_tag="AC16YU_04225"
     CDS             2869..3957
                     /locus_tag="AC16YU_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6064462.1"
                     /translation="MFRLYDRHVARRFGTEYLMLVGALIVFFVVLHYVEFVDDFMDRG
                     ATAREVFTVYYPNYVPEIVRLISPLALFLACIHLTGRLAQRMEIAALQTSGVPLARLM
                     VPYLAVAVVVTGFMFWFNGWVVPETNRVRIAFEMEYTKDAPELTEYNKIHRQTGPGSV
                     LSVGFFDRTDQTARTVTMERFDGTDRLVERIDAARMRWIDSLSVWRVYAPIQRTFGRD
                     GSETLVQRTPFDTVLTLLPRDMARTDGDVDAMTITEASHYIESLERAGADNLGLPLVT
                     YYGKFAYPFANLILVLLGVPLAAVRRRGGQGLVLGLGLLLAFVYLALQKLLEPFGFSG
                     LLPPLLAAVAPHVVFLGVALVALLRARQ"
     gene            complement(3991..4311)
                     /locus_tag="AC16YU_04230"
     CDS             complement(3991..4311)
                     /locus_tag="AC16YU_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR6064463.1"
                     /translation="MSGKETAAGQQQINIELNEEVAEGVYSNLVMIAHSSEEFVLDFI
                     RVMPGVPKARVRSRIIVTPPHAKRLLRAMAENIERYERAHGEIAEMGFPGPAVQFGPP
                     GGEA"
     gene            complement(4308..5732)
                     /locus_tag="AC16YU_04235"
     CDS             complement(4308..5732)
                     /locus_tag="AC16YU_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6064464.1"
                     /translation="MWHDSILGTIGNTPLVRLNRVAEGIPCTVLAKVETFAPGGSVKD
                     RVGIAMVEAAERDGSLRPGGTIIEGTSGNTGAGLALAAIARGYRCIFTTTDKQSQEKI
                     DVLRALGAEVIVCPTAVAPEDPRSYYSVARRLADETPNSVYLNQYDNPANTEAHYRTT
                     GPEVWEQTDGRVTHFIASAGTGGTISGCSHYLKEQNPAIRVVGVDTYGSVYFKYFFTR
                     EFDQREIYTYITEGVGEDILAGNMDFDSVDDYVRVTDRDAQLMTRRLAREEGLFVGQS
                     SGLTVHGAVEWLRANAGELRTDDVVVVLLPDTGFRYLRKTYNDQWMRDHGFLESRPDM
                     TVAQILSGRKGDRRLVSVEAGQPLGEAVSVMTSAGISQVPVMEDGEPVGSLHEGRILK
                     VLVTEPEARDRPVREVMSEPLPVVPESLHLDRLSAHLESESGGVLIHRETGGYFIITK
                     SDLIAALAFGSRDGGRRTTTREHP"
     gene            5862..>6268
                     /locus_tag="AC16YU_04240"
     CDS             5862..>6268
                     /locus_tag="AC16YU_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064465.1"
                     /translation="MITPDPLAGQITELYVATMGTDGRAVYRNRPWIRLLGDSDEPWS
                     RLDPQDALAAAGNVRDAAAGSLVTGQLFLVSSGGRPVPLLLHFLPVRGPDDPNQVVAV
                     AVSGELLVQPESWSSAQTERHRMETLGRMTMGMA"
BASE COUNT          886 a         2269 c         2075 g         1038 t
ORIGIN      
        1 cgggccgaac gggcagggcc gtcatcgctg cagcgcgcgg cctgaccacg cttcggtcac
       61 gccccctcga gttcccaggc gtcctcgacc cggcggaagc ccagccggcg ataccagcgc
      121 gacgtgccgt cgtcccgcac cacgatccga gtgccccgcg accggtccac gatcccctgc
      181 accaggctcc gcccgatccc gagtccctgc acgtcgggct ccacggccac gtcgcacacg
      241 taggtcacgg cgacgccgtc cgtgagcacc cgggcgatgc ccacgagccg gccctggcgc
      301 cacgcggtca ggacgagcga cgaccgctcg aacatgagcc agacccgttc gggggcatcg
      361 gcccgccgcg tgaggggcgc cctccggtac agggtcgtga tccgcgcggg gttcaggccg
      421 tcgagccctt ccctgatttc caggtcttcg atcatgggaa tgcgcccgcc agggcgaggg
      481 tcggtgcaat cagatcgggc cgcggaccca gagggtgcgg gtcgcggcga ggcgcacgtc
      541 gggctgtcga tcgtcgaatc ccgcggtgcg gacacgcagt tcgccggcat cggtccggat
      601 ccgccactcg gtgaacgacc ccaggtaccg gcgggacacc acgcggcccg cgagcggtcc
      661 ctcgggcgcg aacacgaggt gaccggggcg caccgccagc acggtccgtc cgtcgggcgg
      721 gggcccgccc gcgagccgtt cggccgcggg cccctcgacg aggttgctgc ccccgaggaa
      781 tccggcgacg tacgcggtgt cgggccgttc gtatagggcc tccggcgaac cggcctcgac
      841 caggcggcct tcccgcatca cggcgatgcg gtcggacacg gccagggcct cctcctggtc
      901 gtgcgtgacg tagatcgcgg tcgtgcccag ctcctgctgg agctgccgca gctcgtcgcg
      961 cgtccgctcg cgcagggcga cgtccaggtt cgacaggggc tcgtcgaaca gcaggaccgc
     1021 cggctcgacc gccagggcgc gggcgacggc gacccgctgc tgctggccgc cggagagctg
     1081 cgtgaccggc cggtcggcga ggtcctccag gcccacccga ccgagcgttt cgtgcaccct
     1141 ccgggccctc tccctccgat cgacccggcg cacgcgcagg ccgtactcca cgttctcccc
     1201 gaccgtcatg gacgggaaca gcgcgtagct ctgaaagacg agggccgtgg ggcgcgcctg
     1261 gggcgcaacc cgggtgacgt cacggcccgc aatgtggacc gagcccgcgt ccggctgctc
     1321 cagcccggcg acgacgcgca ggagcgtgga tttgccgcat ccgctcggtc ccaggagcga
     1381 aacgaacgat ccggccgcga tgtcgaggct cacgccgtcc agcgcgcgga ccgcgccgaa
     1441 ggtcctgacc aggtccttca ggacgacgtc cgccgcccgc gggcctcccg ttcccggctg
     1501 cgtcttcacg gcgcggtcag gcgagcgcgg ccgtcacctt gtcggccgct tcctggaaca
     1561 ggatggccgt ctcgacctcg agcccgctcc ggtccaggat ctgcttgccc tcctccgcat
     1621 tcgtgccctg caggcgcacg atcagcggca tgtcgatccc gacgttgcgc gcggcctcga
     1681 ccactccgcg cgccacgtgg tcgcaacgca cgatcccgcc gaaaatattg atgaggatcg
     1741 ccttgacgtg gggatccttc aggatgatcc ggaagccggc ttccacggtc tccgggttcg
     1801 cgcctccgcc cacatccagg aagttcgccg gctcgccgcc cgccagcttg atgatgtcca
     1861 tggtcgccat ggcgaggccg gcgccgttca ccatgcagcc cacgttgccg tcgagcttga
     1921 tgtagttgag gctgtgctcg cttgcctcga cctcgagcgg atcctcttcg ttcgtgtccc
     1981 ggagcttgac caggtccggg tgacggtaca gcgcgttgtc gtccaggttg atcttcgcgt
     2041 cgatcgcgag cacctctccg gccggagtga gcacgagcgg gttgatctcc gcgagcgagc
     2101 agtcgctcgc ctcgtatgcc cggtacaggg cgccgatgaa cccgacggcc tgcttgaacg
     2161 ccggtcccgt gaaccccagg ccgaaggcga gcttgcgggc ttggaaaccc tgcaggccga
     2221 tcgacgggtc gatccactcc ttcaggatct tctcgggcgt ttccgcggcg accttctcga
     2281 tctcgacgcc tccctcggtc gaggccatga tcacgggcat gctgcgcgtg cggtcgaggg
     2341 tgatgccgat gtagaactcc ttttcgatgt ccaccgcatc cgcgatcagc acggagcgga
     2401 ccttctgccc ttcgggcccc gtctggtgcg tcttcagcat catgcccagg atgtcgccgg
     2461 cgcgcgagcg gacctcctcg atgccccggg cgagtttcac gcccccgccc tttccccggc
     2521 ccccggcgtg gatctgggcc ttcaccacga acacgtcgcg accggtgtcc gcgccgaccc
     2581 ggcgggcggc ctcgacggcc tcgtccaccg tggtcgccac cacgccgggc tgaaccgcga
     2641 ccccgtagga ggagagaatg tccttggcct ggtactcgtg gatcttcatg gagcggatgg
     2701 tccgggggga cggctggtcg tgaagcgcga aaagtacgag gccgacccgt agggtgccgc
     2761 gcccggacgt gcgaatgccc gcatttcgcc gtttctttgg gcaggtagcg tacgatccac
     2821 atcggacgtg ccgccgtccc ccatccgtcc cgaatccccc tgccgatcgt gttccggctc
     2881 tacgaccgcc acgtcgcccg acgcttcggc acggagtacc tgatgctggt cggcgccctg
     2941 atcgtcttct tcgtggtcct gcattacgtg gagttcgtgg acgatttcat ggaccgcgga
     3001 gccaccgccc gcgaggtgtt cacggtctac tacccgaact acgtgcccga gatcgtgcgc
     3061 ctgatctcgc ccctcgcgct ttttctcgcg tgcatccacc tgaccggacg gttggcccag
     3121 cggatggaaa tcgccgccct gcagacctcc ggggttccgc tggcccgcct gatggtgccg
     3181 tatctcgccg tcgccgtcgt cgtgacgggg ttcatgttct ggttcaacgg ctgggtggtc
     3241 ccggagacga accgcgtgcg catcgccttc gagatggagt acacgaagga tgcgcccgag
     3301 ctcaccgagt acaacaagat ccaccggcag acggggcccg gaagcgtcct gagcgtcggc
     3361 ttcttcgacc ggaccgacca gacggcccgc acggtcacga tggagcggtt cgacgggacg
     3421 gatcgcctcg tggagcggat cgatgcggcc cggatgcggt ggatcgattc gttgtcggtc
     3481 tggagggtgt acgccccgat ccagcggact ttcggtcgcg acggctcgga gaccctggtc
     3541 cagcggaccc ctttcgacac cgtgctcacg ctccttcccc gggacatggc gcgtaccgac
     3601 ggggacgtgg acgcgatgac gatcaccgag gcctcgcatt acatcgagtc cctggagcgg
     3661 gcgggcgcgg acaacctggg cctcccgctg gtgacgtact acggcaagtt cgcgtacccc
     3721 ttcgccaacc tcatcctggt cctcctcggc gtgccactcg cggccgtacg ccgccgcggc
     3781 ggccagggcc tggtcctggg gctcggcctg ctcctggcct tcgtgtacct ggccctgcag
     3841 aaactgctgg agcccttcgg cttcagcggc ctcctgcccc ctctgctggc ggcggtggcg
     3901 ccgcacgtgg tcttcctggg cgtggccctc gtggcgctcc tgcgcgcacg acagtgatcc
     3961 cggagcgggc ctccgggcag cgtcggagtc ctacgcctcg ccaccgggcg gcccgaactg
     4021 cacggcgggc cccggaaagc ccatttccgc gatctccccg tgcgcccgct catagcgttc
     4081 gatgttctcc gccatcgccc gcagcaggcg cttcgcatgc gggggcgtga cgatgatccg
     4141 gctgcgcacg cgggccttgg gcacgcccgg catcacccgg atgaagtcca gcacgaactc
     4201 ctccgacgaa tgggcgatca tgacgagatt cgaatagacg ccctcggcga cctcctcgtt
     4261 cagctcgatg ttgatctgct gctgccccgc ggccgtttcc tttccgctca tggatgctcc
     4321 ctggtcgtag tccgacgccc cccgtcacgg cttccgaagg ccagggccgc gatgaggtcg
     4381 ctcttggtga tgatgaagta gccgcccgtc tcgcggtgga tcagcacgcc cccggactcc
     4441 gactccaggt gcgccgaaag ccggtccagg tgcagggatt ccgggacgac gggaaggggc
     4501 tcggacatca cctcgcggac cggccgatcc cgggcctcgg gctcggtgac gagcaccttc
     4561 aggatgcgcc cctcgtggag gctgcccacc ggctcgccgt cttccatgac gggcacctgc
     4621 gaaatgccgg ccgaggtcat gaccgagacg gcctcgccca ggggctggcc ggcctccacc
     4681 gaaaccagcc ggcggtcccc cttgcgcccc gacaggatct gcgcgaccgt catgtcgggc
     4741 cgggactcca ggaatccatg gtcgcgcatc cactggtcgt tgtaggtctt gcgcaggtac
     4801 cggaatcccg tgtccgggag caggacgacc accacgtcgt cggtgcggag ctccccggca
     4861 ttcgcgcgca gccactcgac ggcgccgtgc acggtcaacc ccgacgactg tccgacgaag
     4921 aggccttcct cgcgcgcgag gcgccgggtc atgagctggg cgtcgcggtc ggtcacccgg
     4981 acgtaatcat ccacggagtc gaagtccatg ttgccggcaa ggatgtcctc gccgaccccc
     5041 tcggtgatgt acgtatagat ctcgcgctgg tcgaactcac gggtgaagaa gtacttgaag
     5101 tacaccgacc cgtacgtatc gacgcccacg acgcggatcg cgggattctg ctccttgagg
     5161 tagtgcgagc agccggaaat ggtgccgccc gttccggcgc tcgcgatgaa gtgcgtgacg
     5221 cgcccgtcgg tctgctccca cacctcggga ccggtggtcc ggtagtgcgc ctccgtgttg
     5281 gccgggttgt cgtactggtt caggtagacg gaattcggcg tctcgtccgc cagccgcctc
     5341 gccacgctgt agtacgagcg cgggtcttcg ggggccacgg ccgtcgggca cacgatcacc
     5401 tcggccccca gggcccgaag gacgtcgatc ttctcctggc tctgcttgtc cgtcgtcgtg
     5461 aagatgcagc ggtatccgcg cgcgatcgcg gcgagcgcca ggcctgcccc cgtgttgccg
     5521 ctcgtcccct cgatgatggt tccgcccggc ctgagcgagc cgtcgcgctc cgcggcctcg
     5581 accatggcga tgcctacgcg atccttgacc gacccgcccg gggcgaacgt ctccaccttg
     5641 gccagtacgg tgcacggaat gccctcagcc acgcggttga gacggacgag aggggtgttc
     5701 ccgatagtgc cgagaatgga gtcgtgccac atgaagccag ggacgccttt gctatattcg
     5761 gtgacctgaa cgtgcggccg gaaggtacga tccaggccgg tcggatggtt ccggccgccg
     5821 cgtccgattc cgcccgcgag cacccgtcgc cccgaaagcc gatgatcaca ccggatcccc
     5881 tggcgggcca gatcaccgag ctctacgtgg ccacgatggg cacggacggc cgtgccgtct
     5941 accggaaccg gccgtggatc cggcttctcg gcgactccga cgagccctgg agccgcctcg
     6001 atccgcagga cgccctcgcg gccgccggca acgtccggga cgccgcggcc ggttccctgg
     6061 tgacgggcca gctcttcctg gtctcgagcg gcggccgccc tgtccccctc ctgctccact
     6121 tcctgcccgt ccgcggcccg gacgatccga accaggtcgt ggccgtcgcg gtctccggcg
     6181 agctgctcgt gcagcccgag tcctggtcca gcgcccagac cgagcggcac cgcatggaga
     6241 cgctcggccg catgaccatg ggcatggc
//
LOCUS       DBNHQZ010000163         7984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2351511, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000163 DBNHQZ010000000
VERSION     DBNHQZ010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2351511"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..585
                     /gene="gcvH"
                     /locus_tag="AC16YU_04245"
     CDS             199..585
                     /gene="gcvH"
                     /locus_tag="AC16YU_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6064466.1"
                     /translation="MKFPADCHYTEDHEWIRLSDDGKTGTVGITDFAQQELGDIVYVE
                     IDALGSDVARNEVFGTVEAVKTVSELFMPVSGHIDEVNPGLEANPESVNDDPYGEGWM
                     IRITLSDPSEVGELLSSEAYAAMVGG"
     gene            593..2161
                     /gene="guaA"
                     /locus_tag="AC16YU_04250"
     CDS             593..2161
                     /gene="guaA"
                     /locus_tag="AC16YU_04250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6064467.1"
                     /translation="MHERIVILDFGSQYTQLIARRIREAGVYSEILPCTTPTSEIHAL
                     EARGIILSGGPSSVYDEDAPELDPAILDLERTDGSPMPILGICYGLQAIAYEMGGAVA
                     RATRREFGRAHLVVDEEAGLFEGVPSGSTVWMSHGDHLVQPPDGYRVIAHTANAPIAA
                     VRSLELPHFGVQFHPEVVHTEHGTHILRNFAYEVCGCAGDWSPESFIDEKTREIREVV
                     GDEHVILGLSGGVDSSVAAVLLHEALGDHLTCIFVNNGLLRKGEWEQVQETFRDHYHI
                     RLEAIDASDLFLGRLQGVTDPERKRHIIGNTFVEVFEEATGSVIERMGRRPRFLAQGT
                     LYPDVIESVSFKGPSATIKTHHNVGGLPDELEFSILEPFRELFKDEVREIGRLLKLPE
                     AMIGRHPFPGPGLGIRIIGPVTREGLEILREADAVFTDELRAQGLYDDVWQAFAVLLP
                     IQTVGVMGDERTYENVCALRAVTSVDGMTADWAPLPHAFLAHVSNRIVNEVRGINRCV
                     YDISSKPPATIEWE"
     gene            2158..3564
                     /locus_tag="AC16YU_04255"
     CDS             2158..3564
                     /locus_tag="AC16YU_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6064468.1"
                     /translation="MMRFVLYRWLPLILLLAAQAAPAAGRQPEPTFDRALEVFRAGAY
                     FDAARDFDAVADRDPSGPRATAARVMAAKALYRAGLYRDAVSALEAFIGRHPSSRYAD
                     DARHTLGLARDAVAASARTPVEMGVVLSLSGSEALQTQALFNGIRMAVDAHNATAQRP
                     VRMVFREIGADPSSAGAAVRTLAAEGIRVVVGALFSDQARAAAEAAQGAGVVFMAPLA
                     TDESVSAGRTLAFQANPTFDVRGRAMAFMAVNGLLLGSLGTISDDEPLGSSRRMAAAF
                     AEEAAALEAEVPVAIRLDTPDDWYRLDAVLRADTLRGIRGLYLPITGEHPDRIAGAVL
                     SSLGRMGLDVRVLGNAAWHDLPIAAQATRYSVTYTNDYLPDISREETAAFDAAYRAFA
                     GVRPDRLVHAGYDVTTFLLEATASTGLDAEATDLADAIRHSPAWEGVAHRIHFEGGQV
                     NRALFYHRYRDGKVELFR"
     gene            3675..4490
                     /locus_tag="AC16YU_04260"
     CDS             3675..4490
                     /locus_tag="AC16YU_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6064469.1"
                     /translation="MRTRFLGTLLLLALAGSGCSGSGRVSYEGPRDAFERGKALFEEE
                     KFDAAIPYLQGVFDFGRTHEWAADAQLMLARAYRANREYLLAANEFTRFTQIFRSDPR
                     VAQASYELAMTYYDRSPQYQLDQTDTEQAIAQFQLFMTRFPDHPLTDEAQVRIVELRD
                     KLAHKRYEAATQYELRGFFEAAALTYESVFDDYYDTTWADDALLGALRAYTSFAKQSV
                     AARQAERLRQAISNYDRLLQIFPDSPVLKEAESAYAEASGMLRAVTAEMESQS"
     gene            4487..5056
                     /gene="nadD"
                     /locus_tag="AC16YU_04265"
     CDS             4487..5056
                     /gene="nadD"
                     /locus_tag="AC16YU_04265"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6064470.1"
                     /translation="MTRIGVFGGSFDPPHLAHLVVAEQARAQAGLDLVLWVPAQRSPL
                     KEDPAPAEAHHRLAMVALAIQGHERFDVWDGELRRPGPSYTVDTLRQLREEHPDWDLH
                     LVIGGDSLEAFGRWRDPDAIRSLARLVVYPRGEALPAPAGERETVLDVPRLAVSSTDL
                     RRRLAAGGSIRYFVPDAVLRYITDHGLYR"
     gene            5086..6648
                     /locus_tag="AC16YU_04270"
     CDS             5086..6648
                     /locus_tag="AC16YU_04270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR6064471.1"
                     /translation="MAGTPTTLRERAAQLIFPRLGSNMPPPVAAERDVERIAAMLAEV
                     PVGGVVLFRGDARRTPEALRRLQRESPFPLLVAADLERGAGQQMAGGTVLPHAMAYAG
                     AGDGAPAMVHHLAWLTAKEALSYGIHWMFAPVADVHSNPANPIISTRAFSSDPQLAGE
                     LVQAFVSGCREGGVYATAKHFPGHGDTDTDSHETVPSVSRSRAALDEVELVPFRAAIA
                     AGVSSVMTAHVAYPGLDASGRIATRSAPILRDLLRGELGFEGVVVSDSLLMAGAGTEA
                     ARQAPELVAAGIDVLLDLEHPALAVDHLVRAVEEGTLPEKTLDEAFRRVWRLKERLVE
                     RHGPDVFTAPPAAGSAPAEAAELAESIADAAIREEGYIPGEWPRAALERGVHVLVFRP
                     IPDYPDPTLSPAEEIFRELLPGATVDTVGPEVPPDEAERLVERARSRGVVVCALVVKP
                     AAWHRFGLRPDQLALIRRLETDAPVLPCSLGSPDVLDHLGDGPYRMCSLSDVSPSVRA
                     AARRIASRLELD"
     gene            6657..7322
                     /locus_tag="AC16YU_04275"
     CDS             6657..7322
                     /locus_tag="AC16YU_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HIR6064472.1"
                     /translation="MAPLFDWLRSERASSVQLAVDDLRDMLRIGEEMFATACANLLDN
                     EILEVDLDVRDDEVDAREAHLRHAALEYLSSSPGRQMSYVLKILGIVQEAERIGDLAK
                     SIADTARLADAPRMAPTVLPIRALRDRVLEAFAETRASFVEHDQARAEGVLQRHDDIK
                     RDANAAIADLAREGHAPTNEAVVLALGARMIGRTSSHLANIASIVVFPYEQIRRSHLD
                     DDT"
     gene            7327..>7984
                     /locus_tag="AC16YU_04280"
     CDS             7327..>7984
                     /locus_tag="AC16YU_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6064473.1"
                     /translation="MRLLPLLMLPLLLASAGCRPPTPDVEALARTIDELVSETPGATV
                     AVVVRDPETGVRYERQPDRLFHAASTMKIPVMIEVYRRVEAGELSLDDSLLVENSFRS
                     IVDSSLFAIGDDSDDAIYLRLGSRMAIRDLVWQMITVSSNLATNLLIDHVGAASVQRA
                     SEALGTTHMQTLRGVEDLKAFEQGLSNRATAADLATLLEAIRDGRAVSPAACRSGAPS
                     S"
BASE COUNT         1146 a         2925 c         2727 g         1186 t
ORIGIN      
        1 gatccccttc caccagcagc tgcttcgcca tccggacttc caggccgggc agttcgacac
       61 ccggttcctg gagcgcttcg agctcgtgga gccggacgcg ccgggcgcct gagcagcggt
      121 tttccgcaca ccggggccgt attatccggt tcgttccctt tcgttcacgc tgagcgccgc
      181 cccgcggcgg tgtgggccat gaagtttcca gccgactgcc attataccga ggaccacgag
      241 tggatccggc tttccgatga cggcaagacg ggcaccgtgg gcattaccga ctttgcccag
      301 caggagctgg gcgacatcgt ctacgtggag atcgacgcgc tcggctccga cgtggcaagg
      361 aacgaggttt tcggaacggt cgaggccgtg aagacggtct ccgagctgtt catgcccgtc
      421 tccggccaca tcgacgaggt gaaccccggg ctcgaagcga atcccgagtc cgtgaacgac
      481 gacccctacg gcgagggctg gatgatccgc atcacgctca gcgatccgtc cgaggtcggg
      541 gagctcctct cgtccgaggc gtacgcggcg atggtcggag gatgagcggc gcatgcacga
      601 gcggatcgtc atcctggatt tcggctcgca gtacacgcag ctgatcgccc gccggattcg
      661 ggaggcgggc gtgtactcgg agatcctccc gtgcacgacg cccacgtccg agatccacgc
      721 gctggaagca cggggcatca tcctgtcggg cgggccgtcg tccgtctatg acgaggatgc
      781 ccccgagctg gaccccgcga tcctggacct cgagcggacg gacgggagcc cgatgccgat
      841 actcgggatc tgctacgggc tgcaggcgat cgcctacgag atgggcggag ccgtggcccg
      901 tgcgacccgc cgcgagttcg gacgcgccca cctggtcgtg gacgaggagg cggggctgtt
      961 cgagggcgtt ccctccggct ccaccgtctg gatgagccac ggcgaccacc tggtccagcc
     1021 gcccgacggc taccgggtca tcgcccacac ggcgaacgcg cccatcgccg cggtccggtc
     1081 cttggagctg ccgcatttcg gcgtccagtt ccaccccgag gtcgtgcaca cggagcacgg
     1141 cacccacatc ctgcggaact tcgcctacga ggtgtgcggc tgcgcgggcg actggtcgcc
     1201 cgaatcgttc atcgacgaga agacgcggga gatccgcgag gtggtgggcg acgagcacgt
     1261 gatcctgggc ctctcggggg gcgtcgattc gtcggtcgcg gccgtcctgc ttcacgaggc
     1321 gctcggcgac cacctgacat gcatcttcgt gaacaacggc ctgctccgaa agggggaatg
     1381 ggagcaggtg caggagacgt tccgggacca ctaccacatc cggctcgagg ccatcgacgc
     1441 ctcggacctg ttcctgggcc ggctccaggg cgtgaccgac cccgagcgca agcgtcacat
     1501 catcgggaac accttcgtcg aggtgttcga ggaggccacg ggctccgtca tcgagcgcat
     1561 gggccgcaga ccgcggttcc tggcgcaggg cacgctctac cccgacgtca tcgagagcgt
     1621 gtcgttcaag ggtccgtcgg cgaccatcaa gacgcaccac aacgtgggcg gccttcccga
     1681 cgagctcgag ttcagcatcc tcgagccgtt ccgggagctc ttcaaggacg aggtccggga
     1741 gatcgggcgc ctgctgaagc tgccggaggc gatgatcggc cggcatccgt ttccggggcc
     1801 gggactcggg atccggatca tcggtccggt cactcgcgag ggactggaga tcctgcgcga
     1861 ggcggatgcc gtgttcaccg acgagctccg ggcgcagggt ctctacgacg acgtatggca
     1921 ggccttcgcc gtgctcctcc cgatccagac ggtcggggtg atgggcgacg agcgcacgta
     1981 cgagaacgtg tgcgcgctcc gcgccgtcac cagcgtcgac ggcatgaccg ccgactgggc
     2041 gccgctcccc cacgcgttcc tcgctcacgt gtcgaaccgg atcgtgaacg aggtgcgcgg
     2101 catcaaccgc tgcgtctacg acatcagctc gaaaccccct gcaacgatcg aatgggaatg
     2161 atgcggttcg ttctctaccg gtggctgccg ctgatcctgc tcctcgccgc gcaggcggcg
     2221 cccgcggccg ggcgccagcc cgagccgacc ttcgaccgcg cgctcgaggt cttccgggcc
     2281 ggcgcgtact tcgacgccgc ccgcgacttc gacgccgtgg ccgaccgcga tccctcgggc
     2341 ccccgggcga cggccgcccg cgtaatggcg gcaaaggccc tgtaccgcgc cgggctctac
     2401 agggatgccg tctccgctct cgaggcgttc atcgggcgcc acccgtcgag ccggtacgcg
     2461 gacgacgcgc ggcacacgct cgggctcgcc cgcgacgccg tcgcggccag cgccaggacg
     2521 cccgtggaga tgggcgtcgt gctctcgctc tccgggagcg aggccctgca gacccaggcc
     2581 ctcttcaacg gcatccggat ggcggtcgat gcccacaatg ccacggccca acggccggtg
     2641 cggatggtgt tccgcgagat cggggccgat ccctcgagcg ccggcgcggc ggtgcggacg
     2701 ctcgcggcgg agggcatccg ggtggtcgtt ggcgcgctct tctccgacca ggcccgcgcg
     2761 gccgccgagg cggcccaggg ggcgggcgtc gtgttcatgg ccccgctcgc gaccgacgag
     2821 tccgtgtcgg cgggccggac gctggccttc caggccaacc cgactttcga cgtccgggga
     2881 cgcgcgatgg ccttcatggc cgtcaacggc ctgctgctcg ggtcgctggg cacgatatcg
     2941 gacgacgagc cgctcggcag ctctcgccgc atggcggccg cgttcgccga ggaagcggcc
     3001 gcgctcgagg ccgaggtgcc cgtcgcgatc cgcctggaca cgcccgacga ctggtaccgg
     3061 ctggacgctg tcctccgggc cgacacgctg cggggaatcc gcggcctgta tctcccgatc
     3121 acgggcgagc acccggaccg gatcgcgggg gcggtgctct cgagcctcgg acggatggga
     3181 ctggacgtcc gcgtgctcgg gaacgccgcg tggcacgatc tgccgatcgc cgcccaggcc
     3241 acccggtatt cggtcacgta caccaacgac tacctccccg atatctctcg cgaggagacg
     3301 gccgccttcg acgcggcgta ccgcgcgttc gccggcgtcc gacccgaccg gctcgtgcac
     3361 gcgggatacg acgtcaccac cttcctgctc gaggcgaccg cgtccacggg actcgacgcc
     3421 gaggccacgg acctggccga cgccatccgg cattcccccg cctgggaggg cgtcgcccac
     3481 cggatccact tcgagggcgg ccaggtgaac cgggcgctgt tctaccaccg ataccgggac
     3541 ggtaaggtgg agctgttccg ctgaccggaa ctgcccgcgg cggcccttcg tatggacggc
     3601 gcgtcggccc cggccgcgat ctcccattcc gcccgtcctg cggacgcaac cgtctccccg
     3661 tccagcgacc ctccatgcgc acccgcttcc tcggcactct cctgctcctc gccctcgccg
     3721 ggtccgggtg ctccggctcg ggccgggtct cctacgaggg accccgcgac gccttcgagc
     3781 gcggcaaggc gctcttcgaa gaggagaagt tcgacgccgc catcccgtac ctgcagggag
     3841 tgttcgactt cggccggacc cacgagtggg ccgccgacgc acagctcatg ctcgcccgcg
     3901 cctaccgcgc caaccgggaa tacctgctcg cggccaacga attcacccgg ttcacccaga
     3961 tcttccgcag cgatccgcgc gtggcccagg cctcgtacga gctcgcgatg acctactacg
     4021 accggtcccc gcagtaccag ctggaccaga ccgacaccga gcaggccatc gcgcagttcc
     4081 agctcttcat gacgcgcttc ccggaccacc ccctcaccga cgaggcgcag gtccggatcg
     4141 tggagctgcg ggacaagctg gcgcacaagc ggtacgaggc cgcgacccag tacgagctgc
     4201 gaggcttctt cgaggcggcg gcgctcacgt acgaatcggt gttcgacgac tactacgaca
     4261 cgacgtgggc ggacgacgcc ctgctcgggg ctctccgggc gtacacgtcg ttcgccaagc
     4321 agagcgtggc cgcccggcag gccgagcgcc tgcggcaggc catttcgaac tacgaccgtc
     4381 tcctgcagat ctttccggac agcccggtgc tgaaggaggc cgagtcggcc tacgcggaag
     4441 cctcgggaat gctgcgcgcc gtgacggccg agatggaatc gcaatcgtga cgcgcatcgg
     4501 ggtattcggc gggtcgttcg accccccgca cctcgcccac ctggtcgtcg ccgagcaggc
     4561 ccgggcgcag gcgggcctgg acctcgtgct ctgggtcccg gcgcagcgct cgcccctgaa
     4621 ggaagacccg gcgccggccg aggcgcacca ccgcctggcg atggtcgccc tggccatcca
     4681 gggccacgag cggttcgacg tctgggacgg cgagcttcgg cggcccggcc cgtcctacac
     4741 ggtcgacacg ctccggcagc tccgagagga gcatcccgac tgggacctgc atctcgtcat
     4801 cggcggcgac agcctcgaag cgttcgggcg ctggcgcgat cccgacgcca tccggagcct
     4861 ggcccgcctg gtggtgtacc cgaggggcga ggcgctgccc gcgccggccg gcgagcggga
     4921 gacggtgctc gacgtgccgc ggctcgccgt ctcgtcgacg gacctccgcc gccgcctggc
     4981 cgccggcggc tcgatccggt atttcgtccc ggatgcggtg ctccggtaca tcaccgatca
     5041 cggcctgtat cgctaacttg gcggacacga ccgactccca tctccatggc gggcaccccg
     5101 accacgctgc gtgagcgcgc ggcacagctc atcttcccgc ggctcggatc caacatgccc
     5161 ccgcccgtgg ccgccgagcg cgacgtcgag cggatcgccg cgatgctcgc ggaggtgccg
     5221 gtcggcgggg tcgtcctttt ccgcggcgat gcgcggcgga cgccggaagc gctgcgccgc
     5281 ctgcagcgcg agtctccctt cccgctcctg gtcgcggccg acctggagcg cggcgccggg
     5341 cagcagatgg ccggcggcac ggtgctcccg catgcgatgg cgtatgcggg agcgggggac
     5401 ggcgccccgg ccatggtgca ccatctcgcc tggctgacgg ccaaggaggc cctctcttac
     5461 ggcatccact ggatgttcgc gcccgtcgcg gacgtccact ccaacccggc caacccgatc
     5521 atttccacgc gggcgttctc gtccgacccg cagctggccg gggagctcgt gcaggccttc
     5581 gtgtccgggt gccgggaggg cggggtgtac gcgacggcca agcatttccc gggccacggc
     5641 gacacggaca ccgattcgca cgagaccgtc ccgtccgtct cccggtcccg ggcggccctc
     5701 gacgaagtgg agctcgtccc cttccgggcg gccatcgccg cgggcgtctc gagcgtcatg
     5761 acggcgcacg tggcgtatcc cggcctcgac gcgtcgggcc gcatcgcgac gcggtcggcc
     5821 ccgatcctcc gggacctgct ccgcggggag ctcggcttcg agggtgtggt cgtgtccgac
     5881 agcctgctca tggcgggcgc cggaacggag gccgcccgcc aggcccccga gctcgtcgcc
     5941 gccgggatcg acgtcctgct ggacctggag caccccgccc tcgcggtcga ccacctggtc
     6001 cgggccgtcg aggagggcac gcttcccgag aagacgctgg acgaagcctt ccgccgcgtc
     6061 tggcggctca aggaacggct cgtcgagcgg cacggaccgg atgtcttcac cgcgccgccc
     6121 gcggccggat ccgccccggc cgaggccgcg gagctggccg agtcgatcgc ggacgcggcg
     6181 atccgcgagg agggatacat cccgggcgag tggccgcgcg ccgcgctgga gaggggcgtg
     6241 cacgtgctgg tgttccgacc gatccccgac taccccgatc cgaccctgtc accggccgag
     6301 gagatcttcc gcgaactgct tccgggagcc accgtggaca cggtgggacc cgaggtcccg
     6361 cccgacgaag cggagcgcct cgtggagcgg gcccgctcgc gcggcgtggt cgtgtgcgcc
     6421 ctcgtggtga agccggccgc ctggcaccgg ttcgggctcc ggcccgatca gctcgccctg
     6481 atccggcgcc tcgagaccga cgccccggtc ctcccctgct cgctcggcag cccggatgtc
     6541 ctggaccacc tgggagacgg gccgtaccgg atgtgctcgc tcagcgacgt gtcgccgtcc
     6601 gtgcgcgccg ccgcgcgccg catcgcctcc cgcctcgaac tggactgaac ggcacgatgg
     6661 ctcccctctt cgactggctc cgatccgaac gcgcctcgtc cgtgcagctg gcggtcgatg
     6721 acctcaggga catgctccgg atcggcgagg agatgttcgc gaccgcctgc gcaaacctgc
     6781 tcgacaacga gatcctggag gtggacctgg atgtgcgcga cgacgaggtc gacgcccgcg
     6841 aggcgcacct gaggcacgcg gccctcgagt acctgtcctc gtccccgggc cgccagatgt
     6901 cctacgtcct gaagatcctg ggcatcgtcc aggaggccga gcgaatcggg gacctggcga
     6961 agtcgatcgc ggacaccgcc cggctcgccg acgcgccgcg catggcgccg acggtccttc
     7021 cgatccgcgc cctccgcgac cgcgtcctcg aggcgttcgc cgagacacgg gccagcttcg
     7081 tggagcacga ccaggcgcgc gccgaggggg tcctgcagcg ccacgacgac atcaagcgcg
     7141 atgcgaacgc cgccatcgcg gacctggcgc gcgaggggca cgcgccgacg aacgaggccg
     7201 tggtgctggc gctcggcgcg cgcatgatcg ggcggacgag ctcgcacctg gccaacatcg
     7261 cctcgatcgt ggtcttcccc tacgagcaga tccgccgctc ccacctggat gacgacacct
     7321 gaatccatgc gccttctccc gctcctcatg cttcccctcc tcctcgcttc cgccggatgc
     7381 cggcccccca ccccggacgt cgaggcgctc gcccggacga tcgacgagct cgtatcggag
     7441 acgccgggcg ccaccgtggc ggtcgtcgtg cgcgacccgg agacgggcgt gcgctacgag
     7501 cgtcagcccg accgcctgtt ccacgcggcc tcgaccatga aaatcccggt catgatcgag
     7561 gtctaccggc gcgtggaggc cggcgagctc tcgctcgacg actcgctcct tgtcgagaac
     7621 tcgttccgct cgatcgtgga cagcagcctg ttcgcgatcg gggacgattc cgacgacgcg
     7681 atctacctgc ggctcgggag ccgcatggcc attcgggacc tcgtctggca gatgatcacc
     7741 gtctcgtcga acctcgccac gaacctcttg atcgaccacg tcggcgccgc ctccgtgcaa
     7801 agggcgagcg aggccctcgg gaccacgcac atgcagacgc tccggggcgt ggaggacctg
     7861 aaggccttcg agcagggcct gagcaaccgg gccacggccg ccgacctggc gactctgctg
     7921 gaggccatcc gtgacggccg cgccgtctcg cccgcggcat gcaggtcggg tgcgccttcg
     7981 tcaa
//
LOCUS       DBNHQZ010000164         5487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3164153, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000164 DBNHQZ010000000
VERSION     DBNHQZ010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3164153"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="AC16YU_04285"
     CDS             <1..1301
                     /locus_tag="AC16YU_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6064474.1"
                     /translation="LRLPEDIVSGTDRGLPLYETLPGESVLFRAVLDQWQAGWLPRLV
                     LTHGGSETRLGLEAVLHRSLRWGRIQEASDGIPAEVVGSGADVRVYSFRGEKAIASVF
                     ASHLARPSADWAVQADLQATWRRYRVFDEAYFGTSFRKPYVFLNPRLGVTWRPEQPLS
                     AFASVALASREPRMKSLYDGEEAGAGFVPAFDRREDGSLDTDRPAVEAERVIDVEAGV
                     SLRRHAWRLSANAFWMEFSDEIVPRGGLDQFGVPRTGNADRTRHVGLELEGAIRLSRG
                     LDLKGNATLSRNRFVRFTEFVTLADWSTAPVARDDRPIAGFPSWIANAELVYVRGGLT
                     ARVAAAGVGRQYIDNGGGEDAEGSRSADLWVDPYVLVDAGVRYAFGAATSLDGLEIAL
                     DVSNLLDARVLQYGNVGFGTPQFFPAATRHAFVGLRYTLR"
     gene            complement(1364..2257)
                     /locus_tag="AC16YU_04290"
     CDS             complement(1364..2257)
                     /locus_tag="AC16YU_04290"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR6064475.1"
                     /translation="MVIDHPDQIAFDRIDGPVVPAPGRVLLTTPEHFEIEYVINPHME
                     GNVGAVDHERAQAQWRALGDAYRKLGFEVSELPGRHGLPDMVFCANQTLPFRRPEDGR
                     TGVVASRMNSVHRAPEVADFVRFFESEGYETIDLPDDIPGSFEGMGDAIWHTGRYLLW
                     GGHGFRTDRRVYDAVSERLNVDILVLSLSDPDFYHLDTCFSVLDRDTVLVYPGAFDEV
                     GLALIHSMFWRVLEAGEHESRRGFACNAHCPDGRHVLIQAGNPETCHLLQHNGFEPVE
                     LETGEFVKSGGSVFCMKQMFW"
     gene            complement(2453..3409)
                     /locus_tag="AC16YU_04295"
     CDS             complement(2453..3409)
                     /locus_tag="AC16YU_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6064476.1"
                     /translation="MVLTPLILQTVTDSLSAGTPPVPAEPASWEVLGTAFWLGVGTTV
                     LHIAVILVAAWLVMRIVGRATRRWASRFEDLPPIHPGRQRAMTVGNLLVSATRYVVWP
                     IAIVTVIAEFGVNIGALIATAGVAGLARGFGAQTLVKDVISGVFLLFDDTIHVGDVIT
                     FNSQTGTVEDVGVRLIRVRRFDGEVLMIPAGELRVFGNRSIEYARVIVEIGVAYEQDV
                     DQVVRELGRIADEWAEETRDIQLDEKPEVQSVLSLGDSSVVVRVVARVVPGEQWQAER
                     LLRHLVKRRFDERGIEIPFPRRTVYVRHETELPERDVSDASE"
     gene            complement(3400..4464)
                     /gene="corA"
                     /locus_tag="AC16YU_04300"
     CDS             complement(3400..4464)
                     /gene="corA"
                     /locus_tag="AC16YU_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR6064477.1"
                     /translation="MPRYVRKRSGKLGKAPGTVEFTGTRRVETAGLTLFRYDEATIDE
                     WSGASFDDVSAGLDGPGTKWLNVDGLHDTDLVQTIGDRFGLHPLTLEDLVSPGQRAKL
                     EEHQGYTFIVARMLHYDPSKPHVESEQVSLVITGDTLLSFQERPGDVFGAVRDRLRSS
                     SGRIRSRGPDYLAYALLDVIVDHYFVTLEAFGDEIEVLEEAVVDNPDPSVQTTMRKLR
                     SNLILVRRAAWPMREVLSAMSRADSPVVTAETVIFVRDAYDHAMHALDVVESLRDVVG
                     GLTDLYMTSLSNRMNDVMKVLTIIGTIFIPLSFIAGVYGMNFDHMPELHLRYGYGLAL
                     FLMAATAGGLLLFFRRKKWF"
     gene            complement(4511..>5487)
                     /locus_tag="AC16YU_04305"
     CDS             complement(4511..>5487)
                     /locus_tag="AC16YU_04305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6064478.1"
                     /translation="QSLGPVAGHLARLLGSPVVFVPDLVGEAAQEAVKLLEPGQVLLL
                     ENTRFDEREKKNDPALSEALAGLADVYVNDAFGSAHRAHSTTEGVTAFVPQSAMGLLV
                     EREVEYLSRLLGEPERPFVGVLGGAKVSDKLGLIENLLDRVDALLIGGAMSYTFLRAL
                     GRGTGSSLVEEDRLAEARALYDRAGGRIQLPTDHVAAEAFAADAPHRTVSGDIPDGLM
                     GVDIGPATLEAYRGVVLGARTVVWNGPMGVFEMEPFAKGTMGMARALADAADAGAMTV
                     VGGGDSVAAVVQSGMQERVSHVSTGGGAMLEFLEGKTLPGIAALTDSD"
BASE COUNT          819 a         1891 c         1948 g          829 t
ORIGIN      
        1 acctgcggct gccggaggac atcgtgtccg ggaccgaccg cgggctgccc ctctacgaga
       61 ccctcccggg cgaatccgtc ctgttccggg cggtcctgga ccagtggcag gccgggtggc
      121 tgcctcgact cgtgctgacc cacggcggat cggagacgcg gctcggactg gaggccgtgc
      181 tgcaccgctc gctgcgctgg ggccggatcc aggaggcttc cgacggcatt ccggcggagg
      241 tggtggggtc cggggccgat gtgcgggtgt actccttccg cggggagaag gcgatcgcgt
      301 cggtgttcgc gtcccacctc gcgcggccgt ccgcggactg ggcggtgcag gccgacctcc
      361 aggccacctg gcgccgatac cgggtcttcg acgaggcgta cttcggcacc tcgttccgca
      421 agccgtacgt gttcctgaat cccaggctcg gggtgacctg gcgtccggag cagccgctga
      481 gcgcgttcgc ctcggtggcc ctggcgtcgc gcgagccccg catgaagtcc ctgtacgacg
      541 gggaggaagc gggagccggg ttcgtgccgg cctttgaccg ccgggaggac ggctcgctgg
      601 acacggatcg tcccgccgtg gaggcggagc gcgtgatcga cgtcgaagcg ggggtctcgc
      661 tgcgccgcca cgcctggagg ctctcggcga acgccttctg gatggagttc agcgacgaga
      721 tcgtgccgcg cgggggcctg gatcagttcg gcgttccgcg gacgggcaac gcggaccgga
      781 cgcggcacgt gggcctggag ctcgagggcg cgatccggct gtcccgggga ctggacctga
      841 agggcaacgc cacgctgtca cgcaaccggt tcgtccgctt caccgagttc gtcacgctcg
      901 cggactggtc gacggcaccc gtcgcccggg atgaccgccc gatcgccggc tttccgtcgt
      961 ggatcgccaa cgcggagctg gtctacgtcc ggggcgggct cacggcgcgc gtggcggcgg
     1021 ccggcgtggg ccggcagtac atcgacaacg gagggggcga ggacgcggag ggcagccggt
     1081 ccgccgacct gtgggtcgat ccgtacgtgc tcgtcgacgc gggcgtccgg tacgcgttcg
     1141 gcgccgcgac gtcgctcgac ggactcgaga tcgcgctcga cgtctcgaac ctgctggatg
     1201 cccgggtcct gcagtacggg aacgtgggat tcggcacgcc gcagttcttt ccggccgcca
     1261 cgcgccacgc gtttgtgggc ctccggtaca cgcttcggta ggggctcgcc gcgcgggccg
     1321 ccgagccgcg ttgccgcccc gggacgcccc ggacctccgc gtcctaccag aacatctgct
     1381 tcatgcagaa gaccgagccg ccggacttca cgaactcgcc cgtttccagt tcgacgggct
     1441 cgaagccgtt gtgctgcagc aggtggcacg tttcggggtt gcctgcctgg atcaggacgt
     1501 ggcggccgtc ggggcagtgc gcgttgcagg cgaagccgcg ccgcgactcg tgctcgccgg
     1561 cctcgaggac gcgccagaac atgctgtgga tgagggcgag ccccacctcg tcgaatgcgc
     1621 ccgggtagac gaggacggtg tcccggtcca ggacactgaa gcacgtgtcc aggtggtaga
     1681 aatcaggatc ggacagggac aggaccagga tgtccacgtt caggcgctcg gagaccgcgt
     1741 cgtacacccg ccggtccgtc cggaagccgt ggccgcccca gagcaggtag cgcccggtgt
     1801 gccagatggc gtcgcccatg ccctcgaagc tgcccgggat gtcgtcgggc aggtcgatgg
     1861 tctcgtagcc ctcgctctcg aagaaacgga cgaagtccgc cacctcggga gcccggtgaa
     1921 ccgagttcat tcggctcgcc accacgcccg tgcgtccgtc ctcggggcgg cggaacggca
     1981 gggtctggtt cgcgcagaag accatgtcgg gcagcccgtg ccgtccgggc agctcgctca
     2041 cctcgaaccc gagcttcctg tacgcgtcgc cgagggcccg ccactgggcc tgggcccgct
     2101 cgtggtccac ggcaccgaca ttgccctcca tgtgcggatt gatgacgtac tcgatctcga
     2161 agtgctcggg tgtcgtcagg aggacgcgtc ccggtgccgg gacaaccgga ccgtcgatcc
     2221 ggtcgaacgc gatctggtcc gggtggtcga tgaccatgaa tctgtgggat atgagcggag
     2281 cctggggcgc tgaaggtatc gccgccgccg gtccccggac aagcgggaat ttacggcgcc
     2341 gggccggacc tcgagcgaca cgccgtccag cgcggtgact acgccccgcc ggaggccccg
     2401 cgcgcaggtc ctgcgcaggt ctgcggcctg gatgacgggg ttcgtcgggg gctcactccg
     2461 acgcgtcgga tacgtctcgt tcgggcagct ccgtctcgtg ccggacgtag accgtgcgcc
     2521 gcggaaaggg aatctcgatg ccgcgctcgt cgaagcgccg cttgaccagg tgccggagca
     2581 ggcgctcggc ctgccactgt tcgcccggca cgacgcgggc caccacgcgg acgaccacgg
     2641 acgagtcgcc caggctcagc acggactgca cctcgggctt ctcgtccagc tggatgtcgc
     2701 gggtttcttc ggcccactcg tccgcgatcc ggccgagctc ccggaccacc tggtccacgt
     2761 cctgctcata cgccacgccg atttcgacga tcacgcgggc gtactcgatg ctccggttgc
     2821 cgaacacccg gagctccccg gccggaatca tcaggacctc gccgtcgaac cggcgcacgc
     2881 ggatgagccg gacgccgacg tcctccaccg tgcccgtctg gctgttgaac gtgatgacgt
     2941 ccccgacgtg gatcgtgtcg tcgaacagca ggaagacccc gctgatgacg tccttcacaa
     3001 gggtctgcgc cccgaagccg cgcgcgaggc ccgccacgcc ggccgttgcg atgagcgcac
     3061 cgatgttcac cccgaactcc gcgatgacgg tgacgatggc gatcggccag accacgtacc
     3121 tcgtggcgga gaccaggagg tttcccaccg tcatggcccg ctggcgcccg ggatggatgg
     3181 gcggcaggtc ctcgaagcgg ctggcccagc gcctcgtggc gcggccgacg atccgcatca
     3241 cgagccaggc cgcgaccagg atgacggcga tatggaggac ggtggtcccc acgccgagcc
     3301 agaacgccgt gccgagcacc tcccacgagg ccggttcggc cgggaccggc ggggtaccgg
     3361 cggacaggga atccgtcacc gtctgaagga tgagcggggt cagaaccatt tcttccgccg
     3421 gaaaaagagg agcaggccgc cggccgtcgc ggccatgagg aagagcgcca ggccgtaccc
     3481 gtaccgcagg tgcagctcgg gcatgtggtc gaagttcatg ccgtacacgc cggctatgaa
     3541 ggagagcggg atgaagatcg ttccgatgat ggtcagcacc ttcatgacgt cgttcatccg
     3601 gttgctcagg ctcgtcatgt acaggtccgt gaggccgccg accacgtccc ggagcgattc
     3661 gaccacgtcc agggcgtgca tggcgtggtc gtacgcatcg cgcacgaaga tcacggtctc
     3721 cgcggtgacc accggcgaat cagcacggga catggcggag agcacctcgc gcatgggcca
     3781 cgccgcccgc cgcaccagga tcaggttgct gcggagcttg cgcatggtcg tctggacgga
     3841 ggggtccggg ttgtcaacga ccgcctcctc gagcacctcg atctcgtcgc cgaaggcctc
     3901 gagcgtcacg aagtagtggt ccacgatcac gtcgagcagg gcgtaggcca ggtagtcggg
     3961 cccgcgcgac cggatgcgtc ccgagctcga ccgcagccgg tcacgcaccg cgccgaacac
     4021 gtcaccgggc cgctcctgga aagagagcag cgtgtcaccg gtgatgacca ggctcacctg
     4081 ctccgactcc acgtgcggct tcgacggatc gtagtgcagc atgcgcgcca cgatgaacgt
     4141 gtagccctgg tgctcctcga gcttcgcgcg ctgccccggc gagacgaggt cctcgagggt
     4201 cagcgggtgc agcccgaagc gatcgccgat ggtctgtacg aggtccgtgt cgtgcaggcc
     4261 gtcgacgttc agccacttgg tgccggggcc gtccagcccg gccgacacgt catcgaacga
     4321 cgcgcccgac cactcgtcga tggtggcttc gtcgtaccga aagagcgtca ggccggcggt
     4381 ttcgacccgc cgggtacccg tgaattccac ggtcccggga gccttgccga gcttgccgga
     4441 gcgcttgcgg acgtaacggg gcatcgggtc ctccctggat cgactcgcgg gaggatacga
     4501 aaggcggcgg tcagtccgag tccgtcaggg ccgcgatgcc gggcagggtc ttgccctcga
     4561 ggaactcgag catcgcgccg ccgccggtcg agacgtggct cacgcgctcc tgcatgcccg
     4621 actgcacgac cgccgcgacg gagtcgccgc cgccgaccac ggtcatcgcc ccggcgtccg
     4681 ccgcgtccgc gagggctcgg gccatgccca tggtgccctt cgcgaacggc tccatttcga
     4741 agacgcccat cgggccgttc cacaccacgg tgcgtgcgcc caggaccacg cctcggtacg
     4801 cctcgagggt ggccggaccg atgtcgacac ccatgagccc gtccgggatg tcccccgaga
     4861 ccgtgcggtg gggcgcgtcg gccgcgaagg cctcggcggc cacgtggtcc gtcgggagct
     4921 ggatgcgccc gccggcccgg tcgtacaggg cccgggcctc cgccagccgg tcctcctcga
     4981 cgagcgaaga gccggtcccg cgcccgaggg cgcgcaggaa ggtgtagctc atggccccgc
     5041 cgatgaggag tgcgtccacg cgatcgagca ggttctcgat gagtcccagc ttgtcggaca
     5101 ccttggcccc accgagcacc cccacgaacg gccgctcggg ctctccgagg agccgcgaga
     5161 ggtattcgac ctcgcgctcg accaggagcc ccatcgcgga ctgcggcacg aatgcggtca
     5221 cgccctcggt cgtggagtgc gcccggtggg cgctgccgaa cgcgtcgttc acgtacacgt
     5281 cggcgaggcc ggcgagggct tccgagagcg ccgggtcgtt cttcttctcg cgctcgtcga
     5341 accgggtgtt ctccaggagc aggacctgtc ccggctccag gagcttcacg gcctcctgcg
     5401 cggcctcgcc cacgagatcg gggacgaaga cgacgggaga gccgagcagc cgggccaggt
     5461 ggccggcgac ggggccgagg ctctgct
//
LOCUS       DBNHQZ010000165         9000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1270607, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000165 DBNHQZ010000000
VERSION     DBNHQZ010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1270607"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2930)
                     /locus_tag="AC16YU_04310"
     CDS             complement(<1..2930)
                     /locus_tag="AC16YU_04310"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR6064479.1"
                     /translation="MPHARNSSPRIDPAPAHRPEDGLVIDRVFSVEGADPFSSVEWER
                     RDAAIRNKEGEAIFEQKDVEFPVSWSPIATNVVASKYFYGEVSRSGEDPARGGRESSL
                     RQLIHRVTRTIADWGTEQGYFASAQDGERFYDELTWLCVNQYGSFNSPTWFNVGLHHQ
                     YGVRDRGGKTLYGWDEETKSVVPVDPYERPQASACFIISVEDSIDDIWQLMAESARLF
                     KFGSGVGADWSMLRSTRETLSGGGTPSGPVSFMRVQDSTGSTIKSGGKTRRAAIMQTL
                     KCDHPDLMEFVVAKQEEEKKAWALIEQGYDGSFNGPAYGSVAFQNVNQSVRVTDAFMQ
                     AAALGGEFPLRAVTTGAEIDRVRAADVLARISEGTWICGDPGLQYEDTIQRWHTCKNT
                     APIHSSNPCSEYMFLDDSACNLASLNLRRFQGTDGRFDVERFLAATRIFITAMEILVD
                     NAGYPSAGIAANSHAFRPLGLGFANLGALLMSMGIPYDSDEGRGVAGAITAIEHCEAY
                     ARSAEIAGNPAIGPFDGYAQNEEPFLEVMRMHRDAVDDIPGACPEYLLRAARESADRM
                     LSYGTRYGYRNAQATVLAPTGTIAFMMDCDTTGIEPDLALVKYKLLAGKGDGQFKIVN
                     RTVPEALTMLGYDDAARDAILAYVEENDTIEGAPRLAAEHLPVFDCAFKPKNGERSIH
                     HLGHIRMMAACQPFISGAISKTVNMPESSSVEDIADAYRKGWELGLKAVAIYRENSKR
                     SQPLATSRGGNTKGAEPAGDGMATGEPRIVERVVERIVARAERHRLPDERPSITHKFS
                     IAGHEGYLHIGLYPDSRMPGEIFITMAKEGSTISGLMDAFATSMSIALQWGVPLEDLC
                     NKFSHMRFEPSGFTNNRQIPIAKSIMDYIARYLSLKFLGAARMAEPEEDAAPQLSVRP
                     ATDAEQMDIFSAPGGSVDALVGATVDVWMQENDRAEAAGRKSAPQAHGSFQNQEDAPP
                     CANCG"
     gene            3460..3861
                     /locus_tag="AC16YU_04315"
     CDS             3460..3861
                     /locus_tag="AC16YU_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064480.1"
                     /translation="MDFTERSAYPHPEEFKVMRPEYLDLEDGYWQASITITPFKVVGR
                     SVSQAGARRAAIFEASKTYRSYHPSYRVDNPYPAEFTDQDGQTWRALTGLMREKNGDY
                     AFTDADGEEDYADIEQMLAWDVRPVLEEEEA"
     gene            complement(3883..4428)
                     /locus_tag="AC16YU_04320"
     CDS             complement(3883..4428)
                     /locus_tag="AC16YU_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064481.1"
                     /translation="MSEPLQETGAVTAARRVVQLVHRVERTAWQVAAWSGASGLFLAV
                     LLAGAARGSSAWLAAPAGLLAVAVLVPAAVLVLLAAGLRQLGRVPDRLFADVTGLARS
                     LPTLTAAESGRAPKRLWSFVRFLLGSRAALAAARDRVLAAGLYLRVLHPAMLVAALVA
                     LAATAVLVPAAVVTAVVIALW"
     gene            complement(4425..6056)
                     /locus_tag="AC16YU_04325"
     CDS             complement(4425..6056)
                     /locus_tag="AC16YU_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR6064482.1"
                     /translation="MPRTDTDPLLERLSAHPLAPLLRLVGEQADRLGIDVCAVGGFVR
                     DIVLGRATPDVDFVTLGAGTGIRLAEAVAGATGGASLHVYENFGTAAIRIPWDGETVV
                     LEFVAARRESYRRESRKPLVEDGTREDDLLRRDFRVNAMALDLAPDRFGTLIDPLGGM
                     QDLATGLLRTPLDPDRTFDDDPLRMVRAARFAAELGFRVNDDAIAAMTRNAPRIGIVS
                     TERVTDEFQRIVCSRRPSTGFLVLEQTGILEHVLPELTDLRGVETVDGQRHKDNFHHT
                     LQVVDNLVDLLGGRACDETRWLRWAALLHDIGKPTTKRFVRGTGWTFHGHEELGARMA
                     GRLFRRLRLPADERLDTVQKLIRLHHRPVSLVDESVTDSAVRRLLFDAGEDVEDLMLL
                     VRADITSKNPARVRRYLRAFDRVEEKMVQVEEKDRLRRFQPPVDGLEIMETLGIPEGL
                     AVGIIKNAIREAILDGEIPNEHDAAFGYMMTVKDDALRRGRLFESVVARLSPRQKRAV
                     GAIKEAVVSPDLPADDHEAEDRLLRLAHGKDGEDS"
     gene            complement(6088..6870)
                     /locus_tag="AC16YU_04330"
     CDS             complement(6088..6870)
                     /locus_tag="AC16YU_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064483.1"
                     /translation="MHFQVDGTSSHADVVRSALEARGLTAAPPGFLPAADAGSCTWVL
                     LDPSDEGVERAADAVRAGWNAVLRWPPDAPASLAERLVGFGEEAGAQVGLLRPLRRLS
                     EIRPLFRGDRPAILTIAVDLPGSESARVLEVTGDLADLASALLGEAGLQRTEIESTPA
                     GGEGFSAVAATVRYRNGSLAQLFVSFGEGAARFHLVAAGGGSVRRAVSLSPGPLPPGG
                     GALARDVVDFVEATGRGSQAPVPLADTIEALRVAERIGATLR"
     gene            7026..7508
                     /locus_tag="AC16YU_04335"
     CDS             7026..7508
                     /locus_tag="AC16YU_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR6064484.1"
                     /translation="MKTRIIVIAAVALVAVVGGLAFGVVDPSTASDKVVVYKTPTCGC
                     CGDWIEHMRGAGFEVEVHDLADLGPVKDQYGVGRTLRSCHTAKVGDYVLEGHVPADQV
                     LRLLAERPDVDGLAVPGMPIGSPGMDEGYDRSTWQDYDVVAFSRNGETEVYAHIQSNP
                     "
     gene            7585..8541
                     /locus_tag="AC16YU_04340"
     CDS             7585..8541
                     /locus_tag="AC16YU_04340"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6064485.1"
                     /translation="MPAPGIILERMAPGLEAVVDAGAPLDTLAEGFSWSEGPVWVPSL
                     GAVLFSDVPENRIHAWSAGAGERVWLEPAGYTGSEPRGGQPGPNGLLLDAEGRLVLCQ
                     HGDRRVARLAAPLDSPSPVFETLADRHGDARLSSPNDAAYRSDGSLWFTDPPYGLAGP
                     EDREVPFNGVYALFPDGRLVVVTDTLTRPNGIAFSPDERTLYVANSDPGKAWWAAYEV
                     AEDGTVGDGRLLVDVTGHVGADEPGLPDGLKVDHAGNLFASGPGGIWVIAPDGEALGR
                     IRLDEPAANVAFGEDGRSLFITASSRLLRTRVLTGRGAASGD"
     gene            complement(8538..>9000)
                     /locus_tag="AC16YU_04345"
     CDS             complement(8538..>9000)
                     /locus_tag="AC16YU_04345"
                     /inference="COORDINATES: protein motif:HMM:NF018178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR6064486.1"
                     /translation="TRAQYADFELEFDWRLEPGGNSGVMFRVTEDRDYPWMTGPEYQL
                     FDNPSGGADLLGIHAAGANYDVHPADTSAAHPAGAWNAGRILVRGNHVEHWLNGAKVV
                     EYELMSPDWLEAVAGSKWSAYPDYGRRAQGHIALQGDHEPVWFRNMRIRRF"
BASE COUNT         1327 a         3098 c         3214 g         1361 t
ORIGIN      
        1 ccgcagttcg cgcagggtgg cgcgtcctcc tggttctgga atgatccgtg cgcctggggg
       61 gcgctcttcc gcccggcggc ttccgcgcgg tcgttctcct gcatccagac gtccacggtc
      121 gcgccgacca gggcatccac cgacccgccc ggcgcggaga agatgtccat ctgctcggcg
      181 tccgtggcgg gccggacgga gagctgcggc gccgcgtcct cttcgggctc ggccatgcgc
      241 gccgcgccga ggaacttcag cgacaggtac cgcgcgatgt agtccatgat ggacttcgcg
      301 atcgggatct ggcggttgtt cgtgaagccg ctcggctcga agcgcatgtg gctgaacttg
      361 ttgcacagat cctcgagcgg gacgccccac tggagggcga tcgacatgga cgtggcgaag
      421 gcgtccatga ggcccgagat cgtcgagccc tccttggcca tcgtgatgaa gatctcgccc
      481 ggcatccggg aatcgggata caggccgatg tgcaggtatc cctcgtgccc ggcgatcgag
      541 aacttgtgcg tgatcgacgg ccgttcgtcc ggcagccggt gccgctcggc ccgcgccacg
      601 atgcgctcga ccacgcgctc cacgatccgg ggctccccgg tggccatgcc gtcgccggcg
      661 ggctcggccc ccttggtgtt gcccccgcgg gacgtcgcga gcggctgact gcgcttagag
      721 ttctcgcggt agatcgcgac ggccttcagg cccagctccc accccttgcg gtacgcgtcc
      781 gcgatgtcct cgaccgagga ggactcgggc atgttcaccg tcttggagat cgcgcccgag
      841 atgaacggct ggcacgccgc catcatccgg atgtgtccca ggtggtggat cgaccgctca
      901 ccgttcttcg gcttgaaggc acagtcgaag acgggcaggt gctccgcggc gagcctcggg
      961 gcgccctcga tcgtgtcgtt ctcctccacg taggcgagga tcgcatcccg cgcggcgtcg
     1021 tcgtacccga gcatcgtgag ggcctcgggg acggtccggt tgacgatctt gaactggccg
     1081 tcgcccttgc cggcaagcag cttgtacttg acgagggcga ggtccggctc gatacccgtc
     1141 gtgtcgcagt ccatcatgaa cgcgatggtc cccgtcgggg ccagcacggt ggcctgggcg
     1201 ttgcggtagc cgtaccgggt gccgtaggag agcatgcggt ccgccgattc ccgggccgcg
     1261 cggagcaggt actcggggca ggcgcccggg atgtcgtcca cggcgtcgcg gtgcatgcgc
     1321 atgacctcga ggaagggctc ctcgttctgc gcgtagccgt cgaacgggcc gatcgccggg
     1381 ttgccggcga tctcggcgct ccgggcgtag gcctcgcagt gctcgatggc cgtgatcgct
     1441 ccggcgaccc cgcggccctc gtcggagtcg tacgggatgc ccatcgacat gagcagcgct
     1501 cccaggttcg cgaagccgag gcccagcggg cggaaggcgt ggctgttggc cgcgatcccc
     1561 gccgagggat agcccgcgtt gtccacgagg atctccatcg ccgtgatgaa gatccgcgtc
     1621 gcggcgagga aacgctcgac gtcgaaccgt ccgtccgtcc cctggaagcg gcgcaggttc
     1681 aggctggcca ggttgcaggc ggagtcgtcc agaaacatgt actcggagca cggattgctc
     1741 gagtggatag gcgccgtgtt cttgcaggtg tgccagcgct ggatcgtgtc ctcgtactgc
     1801 agtcccgggt caccgcagat ccaggtgccc tcggagatcc tggcgaggac gtcggccgcg
     1861 cgcacccggt cgatctcggc gccggtggtc acggcccgga gcgggaattc cccgcccaag
     1921 gcggcggcct gcatgaaggc gtcggtcacg cggaccgact ggttcacgtt ctggaacgcc
     1981 accgaaccgt aggccgggcc gttgaacgag ccgtcgtatc cctgctcgat gagcgcccag
     2041 gccttcttct cctcctcctg cttggcgacc acgaactcca tgaggtccgg gtggtcgcac
     2101 ttgagggtct gcatgatcgc cgcgcgccgc gtcttgccgc ccgacttgat cgtggatccg
     2161 gtcgagtcct gcacccgcat gaacgacacc ggcccggacg gcgtcccgcc gcccgagagg
     2221 gtctcccgcg tggagcggag catggaccag tcggccccga cgcctgatcc gaacttgaac
     2281 aggcgggcgc tctcggccat gagctgccag atgtcgtcga tcgagtcctc taccgagatg
     2341 atgaagcagg ccgaggcctg gggccgctcg tacggatcca ccggcacgac gctcttcgtc
     2401 tcctcgtccc acccgtacag ggtcttgccg ccccggtccc gcacgccgta ctggtggtgc
     2461 aggcccacgt tgaaccacgt cgggctgttg aacgagccgt actggttcac gcagagccag
     2521 gtcagctcgt cgtagaagcg ctcgccgtcc tgcgccgagg cgaaatatcc ctgctccgtg
     2581 ccccagtccg cgatcgtccg ggtcacgcgg tggatgagct ggcgcagcga gctctcgcgg
     2641 ccgccgcggg cgggatcttc cccgctgcgc gacacctcgc cgtagaaata cttgctggcg
     2701 acgacgttcg tcgcgatggg gctccacgag accgggaact ccacgtcctt ctgctcgaag
     2761 atcgcctcac cctccttgtt gcggatggcc gcgtcgcgcc gctcccactc gaccgacgag
     2821 aaaggatcgg cgccctccac ggagaacacg cggtcgatga cgagtccgtc ttcgggacgg
     2881 tgtgcgggcg ccgggtcgat ccggggcgag ctgttcctgg cgtgcggcat gtcgctgggc
     2941 ggtcttcaag gttttcttca gtcgcggcga tcgtctcgcg gcgaggccct gcgcgggagc
     3001 agcggccgtc aggccgccaa tcgggagccg gatttccggg ccgaagaccg tcgctcgaat
     3061 aacggctatt cagaaacgag tgccgcgggt gggggcgagg acggtcgcgc tgggtcaaca
     3121 ttaatccgcg gattccccgt tatgcaagcg ctttttttcc acacttgtcc accggctctg
     3181 aacggcccgt tgtggatcgt gacggcacgg aacgggcgtc ggatggcctc atccgcacga
     3241 tgggaacggg atatgatccg ttattgcgaa aagcaccgcc ggattcggcc cggggcccgc
     3301 gggtggaagg ccatcgtctc gacatcaata acgggcttca cacgttgtca ataacggctc
     3361 ccggccgaac gtccggccgc cggacgagtc ccgaacccgg accggggccg gacgtacaac
     3421 gcggctctga aacccacctg ccgtccgcaa gcgaattcca tggacttcac cgagcgttcc
     3481 gcctatccgc atcccgaaga gttcaaggtg atgcgtcccg agtacctgga cctggaggac
     3541 ggctactggc aggcgtcgat cacgatcacg cccttcaagg tcgtgggccg cagcgtgtcc
     3601 caggcaggcg cgcgccgcgc cgccatcttc gaggcttcga agacctaccg gtcgtaccac
     3661 ccgtcgtacc gcgtggacaa tccgtacccc gcggagttca cggaccagga cggccagacg
     3721 tggcgcgccc tcacgggcct gatgcgcgag aagaacggcg actacgcgtt cacggacgcc
     3781 gacggggagg aggactacgc cgacatcgag cagatgctgg cttgggacgt gaggccggtc
     3841 ctggaggagg aagaggcgta gaccggaggc gccccgcccg cctcaccaga gcgcgatcac
     3901 gacggccgtg acgacggcgg ccggcacgag cacggccgtc gcggccaggg cgacgagcgc
     3961 ggcgaccagc atggcgggat gcagcacccg caggtacagg ccggccgcga gcacgcggtc
     4021 ccgcgccgcc gcgagggccg cacgggatcc caggaggaag cgcacgaagg accagagcct
     4081 cttcggcgcg cggcccgatt cggcggccgt gagggtggga agagacctgg ccagtccggt
     4141 cacgtcggcg aacaggcggt ccggcacacg gccgagctgc cgcagtccag cggcgagcag
     4201 aacgaggacc gcggccggaa cgagcacggc gacggccagc agtcccgccg gcgcggccag
     4261 ccaggcggac gaaccccggg cggcgcccgc gaggagcacg gcaaggaaaa ggcccgacgc
     4321 gcccgaccag gcggcgacct gccaggccgt tcgctccacg cggtggacca gttgcacgac
     4381 gcggcgcgcg gcggtgacgg ctccggtctc ctgcaggggt tcgctcatga atcctcaccg
     4441 tcctttccgt gggcgagccg gagcagccgg tcctcggcct cgtggtcgtc ggcgggaagg
     4501 tccggcgaca cgacggcttc cttgatcgcc ccgaccgccc gcttctggcg cggggagagc
     4561 cgggcgacga cggactcgaa cagccggccg cggcgcaggg cgtcgtcctt gacggtcatc
     4621 atgtagccga aggcggcgtc gtgctcgttc gggatctcgc cgtccaggat ggcctcgcgg
     4681 atggcgttct tgatgatgcc gacggcgaga ccctccggga tgcccagggt ttccatgatc
     4741 tcgaggccgt cgacgggcgg ctggaaccgg cgcagccggt ccttctcctc cacctgcacc
     4801 attttctcct cgacccggtc gaacgcccgc aggtatcgcc ggaccctggc gggattcttg
     4861 gacgtgatgt cggcccgtac caggagcatg aggtcttcga cgtcctctcc cgcgtcgaag
     4921 agcaggcgcc ggaccgccga gtccgtgacg gattcgtcca cgagggagac gggccggtgg
     4981 tgcagccgga tcagcttctg cacggtgtcg agccgctcgt cggccggaag ccgaaggcga
     5041 cggaacaggc gtcccgccat ccgcgcaccg agttcctcgt ggccgtggaa ggtccagccc
     5101 gtgccccgca cgaagcgctt cgtggtcggc ttgccgatgt cgtggagcag ggcggcccag
     5161 cgcagccagc gcgtctcgtc gcacgctctt ccaccgagca ggtccacgag attgtccacc
     5221 acctggagcg tgtggtggaa gttgtccttg tgccgctgtc cgtcgaccgt ctcgacgccg
     5281 cggaggtcgg tgagctcggg gaggacgtgc tccaggattc cggtctgctc caggaccagg
     5341 aaccccgtgg acggcctccg ggagcagact atgcgctgga actcgtcggt cacacgctcc
     5401 gtcgacacga tgccgatgcg gggggcgttc cgggtcatgg ccgcgatcgc gtcgtcgttc
     5461 acgcggaagc cgagctccgc ggcgaagcgg gcggcgcgca ccatgcgcag cggatcgtcg
     5521 tcgaacgtcc ggtcggggtc gagcggtgtc cggagcagcc cggtggccag gtcctgcatg
     5581 ccgccgaggg gatcgatcag cgtcccgaag cggtccggag caaggtccag ggccatcgcg
     5641 ttcacccgga aatcccgtcg aaggaggtcg tcctcgcggg tgccgtcctc gacgagcggc
     5701 ttccgggact cgcgccggta gctctcgcgc cgggccgcga cgaactccag cacgaccgtt
     5761 tccccgtccc acgggatgcg gatggccgcc gtcccgaagt tctcgtacac gtgcagcgag
     5821 gcgcccccgg tggccccggc gacggcctcg gccagccgga tgcccgtgcc cgcgccgagc
     5881 gtgacgaagt cgacatcggg ggtcgcgcgc ccgagcacga tgtcgcggac gaatccgccc
     5941 acggcgcaga cgtcgatgcc caggcggtcg gcctgctctc ccacaaggcg cagaagcggc
     6001 gcgagggggt gggcggacag cctttccagg agcggatcgg tgtcggtacg gggcatgccg
     6061 gctcgcggaa gaaagcgtcg tggccgtcta acgaagggtc gcgccgatgc gttcggcgac
     6121 gcgcagcgcc tcgatcgtgt cggcgagcgg gacgggcgct tgcgagcccc ggccggtggc
     6181 ctcgacgaag tcgacgacgt cccgggcaag ggcgccgcct ccgggaggca gcgggccggg
     6241 agagagcgaa acggcccgcc gcacggagcc acccccggcc gcgaccaggt ggaaccgggc
     6301 ggctccctcg ccgaacgaga cgaaaagctg ggccagcgag ccgttccgat accggacggt
     6361 ggccgccacg gccgagaagc cctctcctcc ggccggcgtc gattcgatct cggtgcgctg
     6421 caggccggcc tcacccagga gcgcagacgc caggtcggcc aggtctccgg tcacctcgag
     6481 gacgcgggcg gactcgctgc cgggcaggtc gaccgcgatg gtcaggatgg ccgggcggtc
     6541 gccgcggaaa agcgggcgga tctcgctcag ccggcggagg gggcgcagga ggcccacctg
     6601 ggccccggcc tcctcgccga acccgacgag acgctccgcc aggctcgcgg gcgcgtcggg
     6661 cggccaccgg agcaccgcgt tccagcccgc gcgaaccgcg tcggcggcgc gctcgacgcc
     6721 ctcgtctgac ggatccagga gcacccacgt gcaggacccg gcgtccgcgg cggggaggaa
     6781 acccgggggc gccgccgtga ggcccctggc ctcgagcgcg gagcgcacga cgtccgcgtg
     6841 gctgctcgtg ccgtctacct ggaaatgcat ggaatgcgcg tcgtcgggcg ccggcgccgg
     6901 gcgcgggttc gttgggtggc cgcaaggtac ccggaggtgc cggagggggc aagagcggcc
     6961 gccggacgga acctttggac ggggggccga ttgtcccccg tcccggaatc aacgtcctga
     7021 gccccatgaa aacgagaatc atcgtcatag ccgcggttgc gctggtcgcc gtcgtcggcg
     7081 ggctggcctt cggggtcgtg gacccctcca ccgcatccga caaggtcgtg gtctacaaga
     7141 cccccacgtg cggatgctgc ggggactgga tcgagcacat gcgcggggcc ggtttcgagg
     7201 tcgaggttca cgacctggcc gacctgggtc ccgtcaagga ccagtacggg gtcgggcgga
     7261 cgctccgttc gtgccacacg gccaaggttg gtgattacgt cctggaaggc cacgtgccgg
     7321 ccgaccaggt gctgcgcctc ctggccgagc ggcccgacgt ggacggcctg gccgttccgg
     7381 gcatgcccat cggctcgccg ggcatggacg agggttacga ccggtccacc tggcaggact
     7441 atgacgtggt cgccttcagc cggaacggag agaccgaggt ctatgcgcac atccagtcga
     7501 atccctgacg cggcagccgt cgcggccccc gcggcgaccc tgctcctggc ggcggcctgc
     7561 gccgagccgc ccccggaatc caccatgccc gcgccgggca tcatcctgga gcggatggcg
     7621 cccgggctcg aggccgtggt agacgccggc gctccgctcg acacgctggc cgaggggttc
     7681 tcgtggtcgg aaggccccgt ctgggtgccc tcgctgggcg cggtgctctt ctccgacgtc
     7741 ccggagaacc ggatccacgc gtggagcgcg ggggccggtg aacgggtctg gctggagccg
     7801 gccggataca cgggatccga gccccgcggc ggccagcccg gtcccaacgg cctgcttctt
     7861 gacgcggaag gccgcctggt cctgtgccag cacggcgaca ggcgtgttgc ccgtctcgcc
     7921 gcgcccctcg acagtccgtc gccggtattc gaaaccctgg ccgaccgtca cggcgatgcg
     7981 cggctgagca gccccaacga cgcggcgtac cgctcggacg gctcgctctg gttcaccgat
     8041 ccgccctacg gcctggcggg tccggaagac cgtgaagtgc ccttcaacgg ggtgtacgcc
     8101 ctcttcccgg acggccggct cgtcgtcgtc acggacacgc tcacgcgacc caacggcatc
     8161 gcgttctctc ccgacgaacg gaccctgtat gtcgccaact cggacccggg gaaggcgtgg
     8221 tgggcggcct acgaggtggc cgaggacggg acggtgggcg atggccgcct cctcgtcgac
     8281 gtcaccggcc acgtgggcgc ggacgagccc ggcctgccgg acggactgaa ggtggaccac
     8341 gccggcaacc tgttcgccag cgggcccggg ggaatctggg tgatcgcccc ggacggggag
     8401 gccctcggcc ggatccgcct ggacgagccg gccgccaacg tcgcgttcgg cgaggacggc
     8461 cgatcgctgt tcatcacggc ctcgtcgcgc ctgctgcgca cccgcgtcct gaccggcagg
     8521 ggggcggcgt cgggggacta gaaccggcgg atccgcatgt tccggaacca gaccggctcg
     8581 tggtcgccct gcagggcgat gtgtccctgc gcgcggcgcc cgtagtccgg gtatgccgac
     8641 cacttgcttc cggcgacggc ttccagccaa tcggggctca tgagctcgta ctcgacgacc
     8701 ttcgcgccgt tgagccagtg ctcgacgtgg ttgccgcgga ccaggatgcg tcccgcgttc
     8761 caggctccgg ccgggtgggc ggcggacgtg tccgcggggt gcacgtcgta gttcgcgccg
     8821 gccgcgtgga tgcccaggag gtcggctccg cccgacgggt tgtcgaagag ctggtactct
     8881 ggcccggtca tccacgggta gtcgcgatcc tcggtcacgc ggaacatgac cccgctgttt
     8941 ccgcccggct cgagcctcca atcgaactcg agctcgaaat cggcatactg cgcgcgggtg
//
LOCUS       DBNHQZ010000166         5337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1133956, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000166 DBNHQZ010000000
VERSION     DBNHQZ010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1133956"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="AC16YU_04350"
     CDS             <1..520
                     /locus_tag="AC16YU_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PstA family ABC transporter permease"
                     /protein_id="HIR6064487.1"
                     /translation="VPSIVHALFGVGAFVLFFKFGTSILAASLTLAVMTLPVVIVATR
                     ESLQAVPMAFREACWNMGATRWQTIRRVVLPNSISGILTGVILEVSRTAGETAPIMFT
                     GAAFFLPFLPQSVYDQTMALSLHLFVISTQVPGVPEGFPYGVALVLIGIVLSMNALSI
                     AFRMVLRGRKKW"
     gene            532..1299
                     /locus_tag="AC16YU_04355"
     CDS             532..1299
                     /locus_tag="AC16YU_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR6064488.1"
                     /translation="MQPKPEIILEVEGLSVSYGGHRALSDISLSVREHEIFGIIGPAN
                     AGKTSFLQAINRMDLFNSSMKVDGSIRFLGRDVGTWRNTFALRRQIGVVFPLPVGLPL
                     SIYENVAFAPRLRGVRAKADLDIVVERSLRRAALWDEVKDRLDSLGSLLSGGQQQRLT
                     IARALSQDPVLLMLDEFSIAVDPVTTMRIEEVLKELRDEITVILVTNLVQQAERLADR
                     TAFFMDGKVIEVGETEALFSGATLHPETTDYIEGRFG"
     gene            1292..2110
                     /locus_tag="AC16YU_04360"
     CDS             1292..2110
                     /locus_tag="AC16YU_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR6064489.1"
                     /translation="MAEPESTPTTDVQAAATAHAAVRTRALNLWYGTFQALFDVDFDV
                     RHGIITSLIGPSGCGKSTLLRCVNRINERLGYVRTEGLVEVLGHDIYRDGIELAQLRK
                     QVGMVFQRPNPLPISIRDNILFGHRLHDPESRDLPRSRADEIVEQALREVLLWDGVKD
                     RLGRRAIELSLEEQQKLCIARLLPVKPKVILMDEPCSALDPKATAAVEELIWQLRGEY
                     TIILVTHNMAQARRASDECAFMLMGRMIEHRRTDDLFVTPRDQRTADYIEGRYG"
     gene            complement(2129..3331)
                     /locus_tag="AC16YU_04365"
     CDS             complement(2129..3331)
                     /locus_tag="AC16YU_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064490.1"
                     /translation="MGEKTGKLIVIDEREESRVPFLRGILTRSLQQAGLPFEDAYEVA
                     SAVRDDLQAEDEIASTHLRRLVAERLKEGGWTEVHARYTRPRDERAVIQVQSRSGELS
                     PLSKGLLGQSMEICALPRDESYAVAAEVEFRLLRDQVTRTTSADVGRLAAHVLRQRIG
                     EKAAERYLRWIVFTHSGEPLILLVGGTTGCGKSTLSAMLAHRLGIVRTQSTDMLREVM
                     RLLVPSKLVPALHQSSFGAHRVLPRWRDDENRTVEAHMIDGYLMQSHEVGIAIEGVFK
                     RAVKEDVSLIIEGVHLAPALQRRLARGAGPVVVPFVLAALKKKRLRRQLVGRGLQVPG
                     RKAQPYLDHFDEIWHLQTFLLSEADRYDIPIVQNTSVEDSVRQVMQTVSDALAERYSA
                     DPADAFPG"
     gene            complement(3419..4273)
                     /locus_tag="AC16YU_04370"
     CDS             complement(3419..4273)
                     /locus_tag="AC16YU_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HIR6064491.1"
                     /translation="MEPETTPPAPAPYPDWWVHAPKEQSLDRALLDRATGPARLLLER
                     LLADEEVYLLHSYANAVSVRRLGYNDHGPVHARITTYNSLKLIQKLHAAGVKTSLEAD
                     QVGTFEDSQAAVVLGCFLHDLGMAVTRDAHEWHSIQLADPFIRRYLDELFPDEPARRV
                     VLRSLAHEVIVGHMGHSRIHSVEAGTVLVADGTDMTRGRSRIPMNMEHDPTVGDMHRY
                     SAGSITRVDITEGDAKPVRITIRMEDRTGLFQVEEVLMGKVKQSPIMGYLEICAIVGE
                     QTPMFYLR"
     gene            complement(4342..>5337)
                     /locus_tag="AC16YU_04375"
     CDS             complement(4342..>5337)
                     /locus_tag="AC16YU_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR6064492.1"
                     /translation="KKISLGMKQLEPDPWENIADRYPPGTVMRGKVRNITNFGVFVEI
                     EPGIDGLVHISDLSWTKKVRHPSEMIRKGQELDVVVLNIDSQDRRISLGHKQVDTNPW
                     NQFGQVYSEGSETEGRVVRVEDKGVVVELPMEVEAFIPQNELRNATHFGSAYREGDEL
                     KGLIVTRFDAAEKEILLSEIAAERAEAYQARQAERRERETERQQERRDIERFSKVAGG
                     ASGPTTLGELSDLAALKKQMQKAEKEAEKKAEAAVEDTAAPVEVEAVTQVEAVTQAEE
                     PQAEEAQAEEAKPEAEEPQAEVAKPKAKRSRKKKEAPADESADSDEADSGEEETE"
BASE COUNT          799 a         1851 c         1737 g          950 t
ORIGIN      
        1 agtgccgtcg atcgtccacg cgctcttcgg cgtcggcgcc ttcgttttgt ttttcaagtt
       61 cggcacgtcg atcctcgccg ccagcctgac gctcgccgtc atgacgctgc cggtggtcat
      121 cgtcgccacc cgcgaatcgc tccaggccgt ccccatggcc ttccgcgagg cctgctggaa
      181 catgggggcg acgcgctggc agacgatccg ccgggtggtg ctgccgaatt cgatcagcgg
      241 catcttaacg ggcgtcatcc tcgaggtttc gcgcaccgcg ggcgagacgg cgccgatcat
      301 gtttaccggc gccgctttct tcctgccttt cttgccacag agcgtctacg accagacgat
      361 ggcgctgtcg ctccacctgt tcgtgatctc cacccaggtg cccggcgtgc ccgagggctt
      421 cccgtacggc gtggcgctcg tcctcatcgg gatcgtcctg tcgatgaacg ccctgtcgat
      481 cgcgttccgc atggtcctga gaggaaggaa gaagtggtag cagacaaacc catgcagccc
      541 aagccagaga tcatcctgga agtcgagggg ctttccgtct cgtacggcgg ccaccgcgcg
      601 ctctcggaca tctcgctgtc cgtgcgcgag cacgaaatct tcggcatcat cgggccggca
      661 aacgccggaa agacgtcgtt cctgcaggcc atcaaccgca tggatctgtt caactcctcg
      721 atgaaggtcg acgggtccat ccgcttcctc gggagggacg tggggacctg gcgcaacacg
      781 tttgcccttc ggcgccagat cggggtcgtc tttccgctgc ccgtcggcct gccgctctcg
      841 atctacgaga acgtagcctt cgctccgcgc ctgcgcgggg tgcgagcgaa ggccgacctg
      901 gacatcgtcg tggagcgctc gctgcggcgc gccgccctct gggacgaggt gaaggaccgc
      961 ctggactcgc tcggctccct gctgagcggc gggcagcagc agcgcctcac gatcgcgcgg
     1021 gcactctcgc aggaccccgt gctgctcatg ctcgacgagt tctctatcgc cgtcgatccg
     1081 gtgaccacga tgcggatcga ggaggtgctc aaggagctcc gcgacgagat caccgtcata
     1141 ctggtgacga acctcgtgca gcaggccgag cgcctcgccg accggacggc cttcttcatg
     1201 gacggaaagg tcatcgaggt cggtgaaacc gaggccctct tctcgggggc cacgctccac
     1261 ccggaaacca cggactacat cgagggacgt tttggctgag ccggaatcca ccccgaccac
     1321 ggacgtgcag gctgcggcga cggcccatgc cgcggtacgc acccgcgcgc tgaacctctg
     1381 gtacggcacg ttccaggcgc tcttcgacgt cgatttcgac gtcaggcacg ggatcatcac
     1441 gtcgctcatc gggccctcgg ggtgtgggaa gtcgacgctg ctccgctgcg tgaaccgcat
     1501 caacgagcgc ctgggctacg tgcgcacgga gggcctcgtg gaggtcctgg ggcacgacat
     1561 ctaccgcgac gggatcgagc tcgcgcagct gcgcaagcag gtcggcatgg tcttccagcg
     1621 gcccaatccg ctgccgatct cgatccgcga caacatcctc ttcggccacc ggctccacga
     1681 tccggagagc cgggatctcc cgcgctcccg ggcggacgag atcgtggagc aggccctgcg
     1741 cgaggtgctg ctctgggacg gcgtcaagga tcgactcggc cgccgggcca tcgagctctc
     1801 gctcgaggag cagcagaagc tgtgcatcgc gcggctgctg ccggtcaagc ccaaggtcat
     1861 cctcatggac gaaccgtgct cggccctcga cccgaaggcc acggccgcgg tcgaggaact
     1921 catctggcag ctccggggcg agtacacgat catcctcgtc acccacaaca tggcgcaggc
     1981 gcggcgggcc agcgacgagt gcgcgttcat gctcatgggc cggatgatcg agcatcgccg
     2041 gaccgacgac ctcttcgtca cgccgcggga ccagcgcacg gccgactaca tcgagggccg
     2101 ctacggctga acgaagggtg ccgtgccctc agcccgggaa ggcgtcggcc ggatccgcgc
     2161 tgtagcgctc cgccagcgcg tcggacaccg tctgcatcac ctggcggacc gagtcctcga
     2221 ccgaggtgtt ctgtacgatc ggaatgtcgt agcggtcggc ctcggaaaga aggaacgtct
     2281 gcaggtgcca gatctcgtcg aaatggtcca ggtagggctg ggccttccgc cccggtacct
     2341 gcaggccgcg gccgacgagc tgccgccgta gccgcttctt cttgagggct gcgagaacga
     2401 acggaacgac gaccgggccc gctccccgcg ccagtctccg ctgcagcgcg ggcgccaggt
     2461 gcaccccctc gatgatcagg gacacgtctt ccttcaccgc gcgcttgaag actccctcga
     2521 tcgcgatgcc cacttcgtgc gactgcatca ggtagccgtc gatcatgtgg gcctcgaccg
     2581 tcctgttctc gtcgtcgcgc caccgcggga gcacgcggtg cgcgccgaaa gaggactggt
     2641 ggagcgccgg gacgagcttc gacgggacca ggagccgcat gacctcgcgc agcatgtcgg
     2701 tcgactgcgt gcggacgatg cccagccgat gcgccagcat cgccgagagc gtgctcttgc
     2761 cgcatcccgt ggttccaccg acgagcagga tgagcggctc cccggagtgg gtgaacacga
     2821 tccagcgcag gtaccgctcg gccgccttct cgcctatccg ctgccggagg acgtgcgcgg
     2881 cgaggcgccc gacgtcggcg ctcgtcgtcc tggtgacctg gtcgcggaga aggcggaact
     2941 cgacctccgc ggcgacggcg taggactcgt ccctgggcag ggcgcagatc tccatggact
     3001 ggcccagcag tcccttggac agcggagaca gctcgccgct ccggctctgg acctggatga
     3061 cggcccgctc gtcgcggggt cgcgtgtacc gggcgtgcac ctcggtccat cccccctcct
     3121 tgagccgctc ggcgacgaga cggcgcaggt gcgtcgacgc gatctcgtcc tccgcctgga
     3181 gatcgtcacg gaccgcggaa gccacctcgt acgcatcctc gaacgggaga ccggcctgct
     3241 gcagcgaacg tgtcaggatg ccgcgcagga agggcacacg gctctcctcg cgctcgtcga
     3301 tgacgatcag cttaccggtc ttctctccca tggaatatcc gcccgacgat gatggctggg
     3361 gtccgacgca ccgtgctcgc cagtatacgc acctgcatcc gatcggagcc ggtcgccgtc
     3421 agcgcaggta gaacatgggg gtctgctcgc cgacgatggc gcagatctcc aggtacccca
     3481 tgatgggtga ctgcttcacc ttgcccatga gcacctcctc gacctggaag aggccggtcc
     3541 ggtcctccat gcggatcgtg atgcggacgg gctttgcgtc cccttccgtg atgtccactc
     3601 gggtgatcga tccggccgag tagcggtgca tgtcgccgac cgtcgggtcg tgctccatgt
     3661 tcatcgggat gcgcgagcgc ccgcgcgtca tgtcggtccc gtcggcgacg agcaccgtgc
     3721 cggcctcgac cgagtggatg cgggaatgtc ccatgtgccc cacgatgacc tcgtgggcca
     3781 gggagcgcag caccacccgc cgcgcgggtt cgtccggaaa caattcgtcc aggtaccggc
     3841 ggatgaacgg gtccgccagc tggatggaat gccactcgtg ggcgtccctc gtcacggcca
     3901 tgccgaggtc atggaggaag cagcccagca cgacggccgc ctgggaatcc tcgaacgttc
     3961 ccacctggtc ggcctcgagc gacgtcttca ctcccgccgc atgcagcttc tggatcagct
     4021 tcagggaatt gtacgtcgtg atccgcgcgt gcaccgggcc gtggtcgttg taccccagcc
     4081 gccggaccga gaccgcgttc gcatacgagt ggagcaggta cacttcctcg tcggcgagga
     4141 gccgctcgag cagcaggcgg gccggcccgg tggcccggtc gagcagtgcc cggtcaaggc
     4201 tctgctcctt gggagcgtgg acccaccagt ccgggtacgg agcgggtgcg ggcggcgtcg
     4261 tttcgggttc catggtatcg gcgtatacgg aaaaggggcg accgggatcg gccgccccct
     4321 tccctcgtca cgggcgcaag gctactcggt ctcctcctcg ccggagtcgg cctcgtcgga
     4381 atccgccgac tcgtcggcgg gcgcttcctt cttcttgcgc gatcgcttgg ccttgggctt
     4441 ggccacttcg gcctggggct cctcggcctc gggcttggcc tcctcggcct gggcctcctc
     4501 ggcctggggc tcctcggcct gggtcacagc ctcgacctgg gtcacagcct cgacctccac
     4561 cggcgcagcc gtatcctcga cggcggcctc ggccttcttc tcggcttcct tctcggcctt
     4621 ctgcatctgc ttcttcagcg cggccaggtc gctcagctcg ccgagcgtcg tggggccgga
     4681 agcaccgccg gccaccttgg agaagcgctc tatgtcacgc cgctcctgct gccgctcggt
     4741 ctcccgctcc cgccgttcgg cctgccgggc ctggtaggcc tcggcccgct ccgcggcgat
     4801 ctcggacagg aggatctcct tctcggcggc gtcgaagcgg gtgacgatga ggcccttgag
     4861 ctcgtctccc tcccggtatg ccgaaccgaa gtgtgtcgcg ttgcggagct cgttctgcgg
     4921 aatgaaggcc tcgacctcca tgggaagctc cacgaccacg cccttgtcct ccacgcgaac
     4981 gaccctgccc tcggtctcgg agccttcgga gtacacctgc ccgaactggt tccaggggtt
     5041 ggtgtccacc tgcttgtgcc cgagcgagat gcggcggtcc tggctgtcga tgttcagcac
     5101 gacgacgtcc agctcctgac ccttccggat catctcgctg gggtggcgca ccttcttcgt
     5161 ccaggaaagg tcgctgatgt ggaccaggcc gtcgatgccc ggctcgatct cgacgaacac
     5221 cccgaaattg gtgatgttgc gaaccttgcc gcgcatgacg gttcccggtg gataccggtc
     5281 cgcgatgttc tcccacggat ccggctccag ctgcttcatg ccgagcgaga tcttctt
//
LOCUS       DBNHQZ010000167         3560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3573365, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000167 DBNHQZ010000000
VERSION     DBNHQZ010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3573365"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2414
                     /locus_tag="AC16YU_04380"
     CDS             <1..2414
                     /locus_tag="AC16YU_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR6064493.1"
                     /translation="SRESRARIGDTVGLGRLLSNLGKVYEALGEYEHAVDAYRESGHL
                     LEEAGDFGRAADARQLAALSLSDYFGSHSEAIVELDQALRLRENADDPEGMIGTLNNQ
                     GILYRRLGNLDGAVAAYQAVIGLAGEQGDTLGVARATNNLGVVFLWAERPDRAATWFG
                     RALEQFEAIGDASGVRDAVVNLASSHFESRQYAEALPFAERALLLSREAGDRVSEGSA
                     MVLLGNVLGYLGEAPAARASYARALERAAELGIPDLEFSTLLSLGEYHERFGDPDSAL
                     VFYDRAFETLEGLRSLLATADDKAGFLAQQRYAYESVIHFLSKRNEAGDDPRHAAAAF
                     AYAERGKARAFLDLLAESLADVSAGISPELKARRETLLSEIAEVRGSVPGAAGQPETA
                     AVLRDSLEALEAEFARLERTLRAENPRYSELQYPEPSTLSDIQAALPDDAVLLEYALG
                     DSSSAVWAITRETARMHRLPPRARIAERVELLRFALADPARGSAAEYADVARALYDEI
                     VAPAADLIGSHPVLVVVPDGALSYVPFEALVVRASDAPEYGNLAYLARTHAVSYAPSA
                     SVLKTLRAGDREADAAAPSGLVAFGDPVFASELAAMQGGPSDPAADLARLPYTGDEVR
                     AIGSLFPEGQATLYLRGDATERVVMDGSALSGFRYVHFATHGIVNEDRPDFSGLALSA
                     VPGAKGEGFLQVAEIFNLRLDADLVVLSACETGLGRMVRGEGLLGLTRAFLYAGASSL
                     VVSLWSVADRSTSELMQRFYASLVKDGESKTAALRTAKLSMLDDPVLAHPFNWAPFTL
                     IGDWQ"
     gene            2538..3110
                     /locus_tag="AC16YU_04385"
     CDS             2538..3110
                     /locus_tag="AC16YU_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064494.1"
                     /translation="MHPFRRSAAFALAVLALLLLLPTDAAAQARAVSLEELVRASNSI
                     ILGRTLGQESFWEDGGKRIMTRVRVAVDETVVGESAAEAEILVPGGRVGDTVYEVSDM
                     PTFVDGEEVLVFVWTSPDGRNLVTGGLQGRMDVVRDAQGRRMVRGLGHLVRSEESPGR
                     AAQETRSSDARPSRAVPLETVLERIRSTRN"
     gene            3276..>3560
                     /locus_tag="AC16YU_04390"
     CDS             3276..>3560
                     /locus_tag="AC16YU_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064495.1"
                     /translation="MPIRSRNLAGAFAALLFLPAAGALAQDPGVWNLGDYSNQLQDCR
                     TLESGSGAAPSFFPEDYLDGTPLAERSCDTWAMDALERPVSADQGIYYPGI"
BASE COUNT          499 a         1273 c         1225 g          563 t
ORIGIN      
        1 cgtccaggga gagccgggcc cggatcgggg acacggtggg gctcggtcgg ctgctctcga
       61 acctgggcaa ggtgtacgaa gcgctgggcg agtacgagca cgcggttgat gcctaccggg
      121 aatcgggcca cctgctggag gaggcggggg acttcggccg ggcggccgac gcgcgtcagc
      181 tggccgcgct ttcgctttcc gattacttcg gaagccattc ggaggccatc gtggagctgg
      241 accaggccct gcgtctccgc gagaacgcgg acgaccccga ggggatgatc ggtacgctga
      301 acaaccaggg catcctgtac cgccgcctgg gcaacctgga cggtgcggtg gcggcgtacc
      361 aggcggtcat agggctggcc ggcgagcagg gcgacaccct gggagtggcc cgcgccacga
      421 acaacctggg cgtcgtattc ctgtgggccg agcgtccgga cagggccgcg acctggttcg
      481 gcagggccct cgagcagttc gaggcgatcg gcgatgcgtc cggcgtccgg gacgcggtcg
      541 tgaacctggc ctccagccac ttcgagtccc gccagtacgc cgaagccctg ccgttcgccg
      601 agcgcgcact gctgctcagc cgggaggcgg gcgatcgggt gtcggagggc tcggccatgg
      661 tgctgctcgg caacgtcctc ggctacctcg gcgaggcgcc cgccgcccgc gcctcgtacg
      721 cccgggccct ggagcgggcg gccgagctcg gcattcccga cctggagttc tcgacgctcc
      781 tttccctcgg cgagtatcac gagcgcttcg gggatccgga ctcggcgctc gtcttctacg
      841 accgggcgtt cgagaccctc gagggcctgc gcagcctgct cgcgacggcg gacgacaagg
      901 cgggcttcct ggcccagcag cgctacgcct acgagtccgt catccacttc ctgtcgaagc
      961 ggaacgaggc cggggacgat ccgcgccacg cggccgcggc gttcgcctac gccgagcgag
     1021 gcaaggcccg ggccttcctg gacctcttgg ccgagtcgct ggcggacgtc agcgcgggaa
     1081 tctcgcccga gctgaaggcc cgccgggaga ccctgctctc cgaaatcgcc gaggtacggg
     1141 ggagcgttcc cggggccgcc ggtcagccgg aaaccgcggc cgtgcttcgg gacagtctcg
     1201 aggccctcga ggccgagttc gcccggctcg aacggaccct ccgggcagag aatccgaggt
     1261 attccgagct tcagtacccg gagcccagta cgctctccga catccaggcg gccctccccg
     1321 acgacgcggt gctgctcgag tacgcgcttg gagactcgag ctccgcggtc tgggcgatca
     1381 cccgtgagac cgcccgaatg catcgcctgc cgccccgcgc ccgcatcgcc gagcgggtcg
     1441 agctcctgcg cttcgccctc gccgatccgg cccggggctc cgcggccgag tacgcggacg
     1501 tggcccgcgc gctgtacgac gagatcgtgg cgccggcagc cgacctcatc gggtcccacc
     1561 cggtgctcgt agtggtgccg gacggggcgc tgtcctacgt gccgttcgag gccctcgtcg
     1621 tgcgcgcgtc ggacgccccg gagtacggaa acctggccta cctggcacgc acccatgccg
     1681 tctcgtatgc cccgtccgcg agtgtcctga agacgctccg tgccggcgat cgcgaagcgg
     1741 acgccgctgc tccgtccggt ctcgtagcct tcggggatcc cgtcttcgcc agcgagctgg
     1801 ccgccatgca gggtggcccg tcggaccctg ccgctgacct ggcccgcctg ccgtacaccg
     1861 gcgacgaggt gcgcgccatt ggcagcctgt tccccgaagg gcaggccacg ctctacctgc
     1921 ggggcgacgc gaccgagcgt gtcgtcatgg acggatcggc gctctccggc ttccgctacg
     1981 tgcatttcgc aacgcacggc atagtgaacg aggaccgccc ggatttctcc ggactggccc
     2041 tgagcgccgt ccccggcgcg aagggcgaag gcttcctgca ggtggccgag atcttcaacc
     2101 tgcggctcga cgccgacctg gtcgtgctct ccgcgtgcga gacgggactt ggtaggatgg
     2161 tgcggggaga gggcctgctg ggcctgaccc gcgccttcct gtacgcgggc gcctcgtcgc
     2221 tggtcgtcag cctgtggagc gtggcggacc ggtcgacttc cgagctcatg caacgattct
     2281 acgcgtcgct cgtcaaggat ggagaatcga agaccgccgc gctccggacg gccaagctgt
     2341 ccatgctcga cgatccggtc ctggcacacc cgttcaactg ggcgccgttc accctgatcg
     2401 gcgactggca atgatcggga cggccaactc cccgttttct ttcgtatata gcggtgcctg
     2461 atccacgccg accgcgtcga tcgggcaccg gcgtcgccac ccccgcaccg ccccctctac
     2521 cccggtaccc ccgaaccatg catcccttcc gccgctccgc agcgttcgcg ctcgcggtcc
     2581 tcgccctgtt gttgctgttg cccacggacg cggccgccca ggcccgtgcc gtctcgctcg
     2641 aagaactcgt tcgggcgtcg aactcgatca tcctgggacg aacccttggc caggagtcct
     2701 tctgggagga cggcggaaag cggatcatga cccgcgtccg cgtggccgtc gacgagacgg
     2761 tggtcggtga gtcggctgcg gaggccgaga tcctggtccc cggcgggcgt gtgggcgaca
     2821 ccgtgtacga ggtgtcggac atgccgacgt tcgtggacgg ggaagaggtg ctcgtgttcg
     2881 tctggacctc gccggacggc cgcaacctgg tgaccggcgg cctgcagggc aggatggacg
     2941 tcgtccggga tgcacagggt cggcggatgg tccgggggct gggccacctg gtccggagcg
     3001 aagaatcccc cggacgcgcg gcgcaggaga cgcgaagctc ggatgcccgg ccgtcccgcg
     3061 ccgtgccgct tgaaacggta ctcgagcgca tccggtccac caggaactga gttccggacc
     3121 ggccgacatc cggctcgtcc cgcggtcctc gcggaattgg ccggagttgg gcacgataga
     3181 ccgggacgac gaatcccggc gccccattct tccctcgttc gatcggacgc cccccaatga
     3241 ctctctgccc ctccatcacg actcttctgt cgcgcatgcc catccgctcg cggaacctgg
     3301 ccggtgcctt cgccgcactc ctcttcctcc cggcagcggg cgctctcgcc caggatcccg
     3361 gcgtgtggaa cctcggggac tattcgaacc agctgcagga ctgccggacg ctggaatccg
     3421 gctccggcgc cgccccgagc ttcttccccg aggactacct ggacggcacc ccgctcgcgg
     3481 aacggtcatg cgacacgtgg gcgatggacg ccctcgagcg cccggtgagc gccgaccagg
     3541 gcatctacta ccccggcatc
//
LOCUS       DBNHQZ010000168         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3165781, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000168 DBNHQZ010000000
VERSION     DBNHQZ010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3165781"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="AC16YU_04395"
     CDS             complement(<1..1328)
                     /locus_tag="AC16YU_04395"
                     /inference="COORDINATES: protein motif:HMM:NF027805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6064496.1"
                     /translation="MPRTLAHFLLLLIAACGRPAAEYPVRIMTVDPGHFHAGLVHGEA
                     YEGVDPVVHVYAPAGPELDRHVAMVEGFNARAADPTSWELVLHTGDDFLERMVEERPG
                     NVMVVAGNNARKIDYVRRAVDAGIHVLADKPMIIRPEDFDVLRLALETADRTGVVVND
                     IMTERHEATSILQAALAHEPDVFGALVPGTQDDPAITKESVHFLSKKVAGSQLVRPAW
                     FLDAAQQGEAIVDVSTHLVDLILWQAFPGSPISYADPADGVRVLEAETWDTELTPAWF
                     ERITQTDAYPDFLWPAVTADSVLHVAANGAFTFSVRGIVARVSVRWGIENPGGGDTHF
                     SRIRGTRADLVIRQDSARGYVPTLYVEPARGEEEAVGRALRASLAGLADEWPGLSVRA
                     SDSGLEVVIPDSLREGHEAHFARVMRRFLADLEAGGLPAWERANLLTKYRV"
     gene            complement(1333..2742)
                     /locus_tag="AC16YU_04400"
     CDS             complement(1333..2742)
                     /locus_tag="AC16YU_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR6064497.1"
                     /translation="MTTKPRPITRREAIRRTLSGAAALVAAPTILPSSVFARPAPSDL
                     IAIGQVGAGRIARDHDLPETMKHRDVRVVAVCDVDHRRMAEGKRFIEERYAREWGAPG
                     AVDVRTEPDYRELVTRSDIDAVVISTPDHGHFLPAAQAALAGKDIYMQKPLSLTIEEG
                     RILSDVCHRMGTVFQVGSQQRSDTPWPQFRRACELVRNGRIGALHTVRIGLPGDPAGG
                     DPTPMPVPDGLDYDMWLGSTPVVPYTVDRVHPVDGYGRGGWLRCEQFSAGMITGWGAH
                     HIDTAHWGMGAEYTGPIEIRAEAEFPAEGLWDVHGPFHVEARYAGGVRMEISGDFPNG
                     VRFEGEDGWIFVTRGAAQVTASDPVSGGGEPALRASDPAILDSVIGPDEIHLVRSEEQ
                     HTNWIRCIRTRAQTVAPVEVAHRSCSACLVSHIAMKLPGTLYWDPVTERFRDNDQANA
                     MRSRPQRHPYTLDLVPGLR"
     gene            complement(2777..>2896)
                     /locus_tag="AC16YU_04405"
     CDS             complement(2777..>2896)
                     /locus_tag="AC16YU_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6064498.1"
                     /translation="DGGRQFFTALGHRPDQYGDPTFLRHILGGIQWAVNGDAP"
BASE COUNT          404 a         1023 c         1039 g          430 t
ORIGIN      
        1 acccggtact tggtcaggag gttcgcccgc tcccacgcgg ggaggccgcc ggcctcgaga
       61 tcggccagga agcgccgcat cacgcgcgcg aagtgcgctt cgtggccctc ccggagcgag
      121 tccgggatga cgacctcgag gccggaatcc gacgcgcgga cggacaggcc cggccactcg
      181 tccgcgagcc ccgcgagcga ggcgcgcagg gcacgcccga ccgcctcctc ctcgccccgg
      241 gccggctcca cgtacagcgt cgggacgtag ccccgggccg aatcctgccg gatcaccagg
      301 tccgcgcggg tgccgcggat gcgggagaag tgcgtgtccc cgccgcccgg gttctcgatg
      361 ccccagcgca cggacacgcg ggcgacgatc ccgcggacgg agaacgtgaa ggctccgttc
      421 gcggccacgt gcaggaccga gtcggccgtc acggcgggcc acaggaagtc ggggtaggcg
      481 tccgtctgcg tgatcctctc gaaccaggcg ggcgtgagtt cggtgtccca ggtctccgcc
      541 tcgaggaccc gcacgccgtc ggccgggtcc gcgtacgaaa tcggagaccc cggaaaggcc
      601 tgccagagga tgaggtcgac caggtgggtc gacacgtcca cgatcgcctc gccctgctgg
      661 gccgcgtcca ggaaccaggc ggggcggacg agctggctgc ccgccacctt cttggacagg
      721 aagtggacgc tttccttggt gatggccggg tcgtcctgcg tcccgggaac gagcgctccg
      781 aagacatcgg gttcgtgcgc cagggccgcc tgcaggatgg aggtcgcctc gtggcgctcc
      841 gtcatgatgt cgttgaccac gacgcccgtc cggtccgccg tctcgagcgc gaggcgcagc
      901 acgtcgaagt cctccggccg gatgatcatc ggcttgtcgg cgagcacgtg gatgcccgcg
      961 tcgaccgccc gtcgcacgta gtcgatcttg cgggcgttgt tgcccgccac gaccatgacg
     1021 ttgcccggcc tctcctccac catgcgctcc aggaagtcgt cccccgtgtg gaggaccagc
     1081 tcccacgagg tcggatcggc ggccctcgcg ttgaagccct cgaccatcgc cacgtgccgg
     1141 tccagctccg ggcccgccgg ggcatatacg tgcaccaccg gatcgacgcc ctcgtaggcc
     1201 tccccgtgca cgagcccggc gtggaagtgg cccggatcga cggtcatgat ccggacgggg
     1261 tactcggccg ccgggcgtcc gcacgccgcg atgaggagga gcaggaagtg tgcgagcgtt
     1321 cttggcatgg cgtcaacgga ggcccggaac gaggtccagg gtgtagggat ggcgctgggg
     1381 cctggaccgc atggcgttgg cctggtcgtt gtcgcggaag cgctcggtga ccggatccca
     1441 gtacagcgtc cccggcagct tcatcgcgat gtgggacacc aggcaggccg agcacgagcg
     1501 gtgggcgacc tcgaccgggg cgacggtctg cgcccttgtc cggatgcagc ggatccagtt
     1561 cgtgtgctgc tcctcgctcc ggaccaggtg gatctcgtcc gggccgatca ccgaatccag
     1621 gatcgcgggg tcgctcgccc ggagcgccgg ctcgccgccg ccgctcacgg gatcgctggc
     1681 ggtgacctgc gccgcgccgc gcgtcacgaa aatccagccg tcctcgccct cgaaacgcac
     1741 gccgttcggg aagtcgcccg agatctccat ccggacgccg ccggcgtacc gggcctcgac
     1801 gtggaacggc ccgtgcacgt cccagaggcc ctcggccggg aactcggctt ccgcgcggat
     1861 ctcgatcggt cccgtgtatt cggcgcccat gccccagtgg gccgtatcga tgtgatgggc
     1921 gccccagccc gtgatcatcc cggccgagaa ctgctcgcag cgcagccacc cgccccgccc
     1981 gtacccgtcc acggggtgca cgcggtccac cgtgtacggg acgaccgggg tcgagccgag
     2041 ccacatgtca tagtcgagcc cgtctggaac cggcatcggc gtcgggtcgc cgcccgccgg
     2101 gtcgcccggc agcccgatcc ggaccgtgtg cagcgcgccg atccggccgt tgcgcacgag
     2161 ctcgcacgcc cttctgaact gcggccaggg cgtgtcggag cgctgctggc tgccgacctg
     2221 gaagacggtt cccatccggt ggcacacgtc cgacaggatg cggccttcct cgatcgtgag
     2281 cgagagcggc ttctgcatgt agatgtcctt gcccgcgagg gccgcctgcg ccgccggaag
     2341 gaagtgcccg tggtcggggg tcgagatcac gaccgcgtcg atgtcgcttc gtgtgacgag
     2401 ctcccggtag tcgggctccg tgcgcacgtc gaccgcgccg ggcgcgcccc actcgcgcgc
     2461 atagcgctcc tcgatgaatc gcttgccctc ggccatgcgc cgatgatcca cgtcgcagac
     2521 ggcaacgacc cggacatcgc ggtgcttcat ggtttcgggc aggtcgtggt cccgcgcgat
     2581 ccgccccgcg ccgacctggc cgatcgcgat gaggtcggag ggcgccgggc gggcgaacac
     2641 cgacgacggg aggatcgtgg gtgccgcgac cagcgccgcg gctcccgaca gggtccgccg
     2701 gatggcttcc ctgcgggtga tgggacgagg cttcgtcgtc atgctcggga tcgggttgcg
     2761 ggcgggcggg actgcatcag ggagcgtctc cgttgacggc ccactggatg ccgcccagga
     2821 tgtggcgcag gaatgtgggg tcgccgtact ggtcgggcct gtggccgagc gccgtgaaga
     2881 actggcgccc gccgtc
//
LOCUS       DBNHQZ010000169         4499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_322372, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000169 DBNHQZ010000000
VERSION     DBNHQZ010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_322372"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..3478
                     /locus_tag="AC16YU_04410"
     CDS             326..3478
                     /locus_tag="AC16YU_04410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6064499.1"
                     /translation="MKGILRSISLLAILLSTCAATTAFGQSGKIAGRVTDAATGEPLP
                     GVNVVVEETMQGTVTDLDGYYVILNVTPGTVSLRVSFVGFTTQTIEDLRIQIDQTTTV
                     NVVMAEEIIQGQEVVITAERPVVQRDVSNSQLNVSSEQIEALPVSSVSGVVGLQAGIQ
                     GLSVRGSGTDELSFNINGLTLRDERDNTPYTNIALTSVEEVQVQTGGFNAEYGNVRSG
                     VVNVITKEGSRSRYEVDAILRYSPPAQKNIGQLANDPDAYWIRPFTDPNVAWTGTDNP
                     VDPTEWDEFIDAQYPDFRGWVTVSEELLSDDDPSNDMTPEALQQAFLWQHRKEMEITE
                     PDYNVDLGIGGPVPGLRNVGNTRFYASFRRDQSMYAIPLYTDRSEAQSGHLKLTSDVG
                     PGMKLTIEGMLGSSEGTASSRAGAPGLFSSPFSIASQLSRVSFIDTRIFSTDYWTPYE
                     VKTNLLGAKFTHSPSNTSFYEIRFTRFGTDYDSNPGPLRDTTAVVDFGGVGFDEAPFG
                     FQPDPSFGVDGMRMGVGMSNSRDSSKVVVYNLKGDYTNQLNRFLEIKTGVELNLTDSR
                     VNYGSFDAYLKSSNSWSHWDRTPARGAAYAQGKLEFQGMIANAGLRLDWFRAGGDWYV
                     IDDPFTPAFSSARAAGLDTLLAKEPTDMITTLSPRLGVSFPITSGSKLFFNYGHFRSM
                     PDPNNLFLIRPFTETGQIARIADPNNPLPKTVAYEIGFEQAIADQFLLRVAGYYKDVA
                     LQPFLVSYTSRDGQTSYTTTEPNSYEDIRGFEITLARNQGRWLQGFVNYTYMVTTSGY
                     FGFASISENRTAQREYEASDAVRRAASNRPVPQPYARLNLDFLVPEDFGPRMGTLNPL
                     GGWRASVIASWQAGAKFTWAGGGSKPGVLNNTQFRDNWNVNLRLTKNFTVKGRRAQFF
                     VDIYNVFNRRTLTFNGFVDGVDQNAYLLSLHLPESPDYPNIPGDDRIGDYRKDGVAYQ
                     PMVNVTSRDQVGAPKPQAIYWESETRSFIEYADGAWQEVDDARLDRVLEDKAYIDMPN
                     QGFLTFLDPRDIYWGVRFTF"
     gene            3524..>4499
                     /locus_tag="AC16YU_04415"
     CDS             3524..>4499
                     /locus_tag="AC16YU_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064500.1"
                     /translation="MTIRRLTVAGLAIAALAAVQPARAQFVNKWLSAGSLHNWYSEVG
                     QEVEVRGFVGNQQDGLRWPGIYRYRDSQAAKGLWIGAKNVTDDQGTNWATRVVHVGPR
                     VLGSGEFFPVRFDMISRFDPPLVFVDGEVTEPEAAMVNDLVDPTIKADRMIVNEVNTL
                     LGLTMERRILQFSQDWHDNYHVIEYTFTNTGNTDSDSAIELPNQTLEDVVVFLQWRWS
                     VAAESRYVIGNGTGWGMNAMLDTRGDGVKADPPGEQFRAQFVWHGKFTAFTLYDNVGG
                     PILKSALPAVQIAASDTLGRLGASQFVGAVTLHADQSPTNRVDDPSQPS"
BASE COUNT          827 a         1487 c         1445 g          740 t
ORIGIN      
        1 cgagaagctg cgccggcggg cgctggcccc gatcgagcgg atgatggcga tgagcgaggg
       61 ggtgcggtag tcgcctggac agggcgccgg aagccggtcg atcgccgcgc aggcgtccgg
      121 gaggcgccgc gcgacgtgca ccgaaaggag cggttccgcg gcggggcatt gccgacaggt
      181 ctgtagggag catctcccga cctgaaaaag ggcaccgacg agtgcccccg aatcagttta
      241 cttcctgccc gggatgcctc atattcggtt catacacggc gcctcggccg cgccggcatc
      301 ggaatcatca ggaccggatc gtgccatgaa gggcatcctt cggagcatct cgctgctcgc
      361 catcctgcta agtacctgcg ccgcaacgac cgcgttcggt caatcgggca agatcgccgg
      421 tcgggtcacc gacgccgcca cgggcgagcc gctgcccggc gtgaacgtcg tggtggagga
      481 aacgatgcag ggtaccgtca ccgacctcga cggatactac gtgatcctga acgtcacgcc
      541 cggcacggtc tcgctgcgcg tctcgttcgt cggcttcacg acgcagacga tcgaggacct
      601 gcgcatccag atcgaccaga ccacgacggt caacgtcgta atggccgagg agatcatcca
      661 gggccaggag gtggtgatca ccgccgagcg gcccgtagtg cagcgggacg tctcgaacag
      721 ccagctgaac gtctccagcg agcagataga agcgcttccg gtgtcgagcg tgtcgggcgt
      781 cgtcgggctc caggcgggca tccagggtct ttccgtacgc ggcagcggca cggacgagct
      841 ctccttcaac atcaacggcc tcacgctccg cgacgagcgc gacaacaccc cgtacacgaa
      901 catcgccctg acgtcggtcg aggaggtcca ggtacagacg ggcggcttca acgcggagta
      961 cgggaacgtg cgctcgggcg tcgtgaacgt gatcaccaag gaggggtcgc gctcgcgcta
     1021 cgaggtcgac gccatcctgc gctacagccc gcccgcccag aagaacatcg ggcagctggc
     1081 caacgacccg gatgcgtact ggatccggcc gttcaccgac ccgaacgtgg cctggaccgg
     1141 taccgacaat ccggtcgatc cgaccgagtg ggacgagttc atcgacgcgc agtacccgga
     1201 cttccggggc tgggtcacgg tgtccgagga gctgctctcg gatgacgatc cctcgaacga
     1261 catgaccccc gaggcgctgc agcaggcctt tctgtggcag caccgcaagg agatggagat
     1321 caccgagccc gactacaacg tggacctggg catcggcggg cccgtgccgg gcctgcgtaa
     1381 cgtcgggaac acgcgcttct acgcgagctt ccgtcgcgat cagagcatgt acgcgatccc
     1441 gctttacacg gaccgaagcg aggcccagtc gggacacctg aagctgacgt ccgacgtggg
     1501 gcccggcatg aaactgacga tcgagggcat gctgggttcg agcgagggta ccgcctcgag
     1561 ccgcgcgggc gccccggggc tattctcctc gcccttctcg atcgcgtcgc agctgagccg
     1621 cgtgagcttc atcgacacgc gcatcttctc cacggactac tggacgccgt acgaggtcaa
     1681 gaccaacctg ctcggcgcca agttcacgca ctcgccgtcg aacacatcct tctacgagat
     1741 ccggttcaca cgcttcggta cggactacga ttcgaacccg ggtccgctcc gggacaccac
     1801 ggccgtggtc gacttcggcg gcgtgggctt cgacgaggcc ccgttcggtt tccagcccga
     1861 tccctcgttc ggcgtcgacg gcatgcggat gggtgtcggc atgtcgaaca gccgcgattc
     1921 ctcgaaggtc gtcgtctaca acctgaaggg cgactacacg aaccagctga accgcttcct
     1981 cgagatcaag accggtgtcg agctcaacct gacggactcg agagtgaact acggcagctt
     2041 cgacgcctac ctgaagtcga gcaattcgtg gtcgcactgg gaccgcacgc cggcgcgcgg
     2101 cgcggcctac gcccagggca agctggaatt ccagggcatg atcgccaacg cgggtctgcg
     2161 gctcgactgg ttccgcgccg ggggcgactg gtacgtgatc gacgacccgt tcacgccggc
     2221 gttctcgtcc gctcgcgctg ccggcctcga cacgcttctc gccaaggagc cgacggacat
     2281 gatcacgacc ctgagcccgc gtctcggggt ttcgttcccg atcacatccg gctcgaagct
     2341 cttcttcaac tacgggcact tccgctccat gcccgacccg aacaacctgt tcctgatccg
     2401 ccccttcacc gagacgggac agatcgcgcg catcgcggat ccgaacaacc cgctccccaa
     2461 gacggtggcc tacgagatcg gattcgagca ggcgatagcg gaccagttcc tgctgcgcgt
     2521 ggcggggtat tacaaggacg tggccctgca gccgttcctg gtttcgtaca cgagccggga
     2581 cggccagacg agctacacca cgaccgagcc caattcgtac gaggatatcc gtgggttcga
     2641 aatcacgctt gcgcgaaacc agggacgctg gctgcagggt ttcgtgaact acacctacat
     2701 ggtgacgacc tcgggttact tcgggttcgc cagcatcagc gaaaatcgca cggcgcagcg
     2761 cgagtacgag gcgtcggacg cggtccggcg cgccgcctcg aaccggccgg tgccccagcc
     2821 ctacgcccgc ctgaacctcg atttcctggt tcccgaggac ttcggcccac ggatggggac
     2881 cctgaatccc ctgggtggct ggcgggcgag cgtcatcgcc tcgtggcagg cgggggctaa
     2941 gttcacgtgg gcgggcggcg gctcgaagcc tggcgtgctg aacaacacgc agttccgcga
     3001 caactggaac gtgaacctgc ggctgaccaa gaacttcacc gtcaaggggc gccgcgccca
     3061 gttcttcgtg gacatctaca acgtcttcaa ccggcgcacg ctcacgttca acgggttcgt
     3121 ggacggtgtg gaccagaacg cgtacctcct ctcgcttcac ctgcccgagt caccggacta
     3181 tccgaacatt ccgggagatg accggatcgg agattaccgg aaggacggcg tcgcgtacca
     3241 gccgatggtc aacgtgacgt cgcgcgacca ggtgggcgct ccgaagccgc aggccatcta
     3301 ctgggagtcc gaaacccggt ccttcatcga gtatgcggat ggtgcatggc aagaggtcga
     3361 cgacgcccgc ctggaccgcg tgctcgagga caaggcctac atcgacatgc ccaaccaggg
     3421 cttcctcacc ttcctcgatc cgagggacat ctactggggc gtccgcttca cgttctgaca
     3481 cccgatgact ccgaatcgcg aaacgacgct ctccatggtt gccatgacaa tccgacgtct
     3541 gacggtggcc ggcctggcga tcgcggcact ggctgccgta cagcccgccc gtgcccagtt
     3601 cgtaaacaag tggctttcgg ccggctcgct gcacaactgg tattccgagg tgggccagga
     3661 ggtcgaggtg cgcggcttcg tgggcaatca gcaggacggc ctgcgctggc cgggcatcta
     3721 ccggtaccgg gattcgcagg cggccaaggg tctctggatc ggtgcgaaga acgtcacgga
     3781 cgaccaggga acgaactggg cgacgcgcgt cgtgcatgtg ggcccccgcg tgctcggctc
     3841 gggcgagttc tttcccgtgc gcttcgacat gatcagccgc ttcgatcctc ctctcgtgtt
     3901 cgtggacggg gaggtgaccg aacccgaggc cgccatggtg aatgacctcg tcgatccaac
     3961 gatcaaggcg gaccgcatga tcgtgaacga ggtgaacacg cttctcggtc tcaccatgga
     4021 gcgcaggatc ctccagttca gccaggactg gcacgacaac taccacgtga tcgagtacac
     4081 cttcaccaac acgggaaaca cggactccga ttcggcgatc gagctgccga accagaccct
     4141 cgaggacgtg gtcgtctttc tgcagtggcg ctggtcggtg gccgccgagt cccgctacgt
     4201 catcgggaac gggacaggct ggggcatgaa cgcgatgctg gacacccgcg gggacggcgt
     4261 gaaggcggat cctcccgggg agcagttccg cgcccaattc gtctggcacg gcaagtttac
     4321 tgcgttcacc ctctacgaca acgtgggcgg tcccatcctg aagagcgcac tgcccgcggt
     4381 ccagatcgcg gcctccgaca cactgggccg cctgggcgcc tcgcagttcg tgggtgcggt
     4441 gacgctgcac gcggaccagt cgccgacgaa ccgggtagac gacccctcgc agccttcga
//
LOCUS       DBNHQZ010000170        10952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_865115, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000170 DBNHQZ010000000
VERSION     DBNHQZ010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_865115"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="AC16YU_04420"
     CDS             complement(<1..702)
                     /locus_tag="AC16YU_04420"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HIR6064501.1"
                     /translation="MSDRRIRVGLVQMAMTDDPVANTGRALDLTREAVAGGAQVVCLP
                     ELFRSRYFCQSEDPAHFDLAEPVPGPTTDLFEPLARESGAVLVLSLFERRAPGLYHNT
                     AAVLDGERGYVGKYRKMHIPDDPRYYEKYYFTPGDLGFLAWPTGAGTLGVLVCWDQWY
                     PEAARLTALQGAEVLFYPTAIGWHPEEADTHGAAQHGAWETIQRSHAVANGCFVVSVN
                     RTGFEPDPAGGGGIRF"
     gene            complement(699..2705)
                     /gene="speA"
                     /locus_tag="AC16YU_04425"
     CDS             complement(699..2705)
                     /gene="speA"
                     /locus_tag="AC16YU_04425"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HIR6064502.1"
                     /translation="MSEVGVVTDTEAPREAGWQPSDAQARYHIQGWSDGYFRVDDRGR
                     MVADVRRDGSAAIAISEVVSQLREDGVPFPVLIRFQDIIRGRVEQLNAEFEAARAEFE
                     YEARYRGVYPIKVNQLHEVVEEILEAGRPFGIGLECGSKAELVATLPHLTDDDTLLIV
                     NGYKDSVMMELILTGQQLGKQVIPVLEKYDEFERLLRLAAKRRIKPRFGVRVRLNTGG
                     SGKWADSGGDLSKFGISIPELLRLLTRLEEDELDEAFVLLHFHLGSQVNDIQTLRKGI
                     KEITQIYAQLVKRDVPIRFLDVGGGLGVNYEADYTGGTSEGINYTLQEYANSVVAQVK
                     EVCDSEGVAQPTLITESGRALTAHHSVLVVEVLGAYRKDEIPDGFEPADEDHAVVKQL
                     FETLSWIREDRQLRPGALLEAYHDAAAKRQEAESLFGFGYLSIEQKALAEQLYWTICQ
                     SLNNRVRGANADWLPAELEALDEHLVDQYLCDFSVFQSILDHWSFGQRFPIVPIERLD
                     EEPDRRAVLVDLTCDSDGKVRRYVSANDDKRFLDVHALRDGEPYLLGFFLMGAYQDIM
                     GDMHNLFGRVAEVHVYADDHEAEGFYIEKLIPPTSVEEILALVQYFPKDLQKRMEGIM
                     RGKVEDGTLRPRAAVRLLEEYGAAFRESTYFNTAADRRRDRSGR"
     gene            complement(2702..3733)
                     /locus_tag="AC16YU_04430"
     CDS             complement(2702..3733)
                     /locus_tag="AC16YU_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-hypusine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HIR6064503.1"
                     /translation="MTITDFIRHHYRHFNAAALVDAAQGYVDHLEGGGRMMVTLAGAM
                     STAELGLSLAGMIRAGKVHAISCTGANLEEDLFNLVAHDHYVRVPRYRDLRPEDEQAL
                     LERHLNRVTDTCIPEEEAIRRIERQVLDLWQAADRSGERRFPHEFLYELVLSGVLSAS
                     YQVDPADSWLVAAAERNLPIVVPGWEDSTLGNIFAGHCIKGDVRDPRTVRSGIEYMMV
                     LAEWYERESAGHSIGFFQIGGGIAGDFPICVVPMLEQDLGRNTPKWGYFCQISDSTTS
                     YGSYSGAVPNEKITWGKLSIDSPSYIIESDATIVAPLVFAYVLESDRAAAAEAARLAG
                     RGVDSEVDR"
     gene            complement(3767..4258)
                     /locus_tag="AC16YU_04435"
     CDS             complement(3767..4258)
                     /locus_tag="AC16YU_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064504.1"
                     /translation="MDTVTQTGVVPETPFPLPQILDLDPDEEPEADDDAEPDEDDEIG
                     DLDEDEDWEEDDEDEDDWDEDEDEDEDDWDEDEDEDEVEDVDEDAEGGDWIEGGDDDW
                     DDEPLLETRFRGLTEDLDEPRPRIPRPGRGLPRDRRESRPRDRNRRGSGLGLGEEDDD
                     ALD"
     gene            4883..6142
                     /locus_tag="AC16YU_04440"
     CDS             4883..6142
                     /locus_tag="AC16YU_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6064505.1"
                     /translation="MKRAPKSDYLVLFPLWLMVFSASSQVIIISPILPRIGEALMVPD
                     EHLGWLITAYATFLSVFALIIGPISDRVGRRRVLLIGSASMTFALMLHGLADSFTSLL
                     VVRSVAGAAGGMLSGGAVSYVGDYFPYERRGWANGWVMSGIAVGQIIGIPIGTLIAAS
                     MGFRAAFMLFAITMGFATLLIALFVPQPDVARSTGRLTVVGSLRAYWQLLRRPVIVAA
                     TIVYFLMFFSLGLYVIYLPTWIERSLSVQGTAIASLFLVGGIANVISGPVAGRISDSI
                     GRKPVIVASCVGLAIVMIATTHLVHSMGMAYIVFALAMAMVAMRVSPLQSLMTELVTA
                     DRRGALMCLAVALGQFGIGIGGAAAGPAYTHFGYASTTYAGAVAIALMAFMVQRFLPE
                     PSAHYRPVGRRVDGQSGSEETASAKAS"
     gene            complement(6100..6816)
                     /locus_tag="AC16YU_04445"
     CDS             complement(6100..6816)
                     /locus_tag="AC16YU_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01548 family HAD-type hydrolase"
                     /protein_id="HIR6064506.1"
                     /translation="MATIRAALFDLDGVLVDVSGSYRLAIRATAQHFTGIPVGDAAVQ
                     RLKDSGGYNDDWRVTAELIARTGTRVAFADVVREFNLRYRGPDWDGLIAAEPALVTAR
                     QLDALRDRFGSLGLVTGRPREEARFTLDRFGWTDRFDVLVTMEDAVGRGKPDPRPLQI
                     AFERLASLGRAVDPGEAVYVGDSVDDMTAARAAGCRAIGFVPPYLDAGRHASLLRDRG
                     AHAVIVDHDALADAVSSLPD"
     gene            complement(6827..9142)
                     /gene="rho"
                     /locus_tag="AC16YU_04450"
     CDS             complement(6827..9142)
                     /gene="rho"
                     /locus_tag="AC16YU_04450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6064507.1"
                     /translation="MRIADLQAKTISDLKEIARQMNLSGFSTLRKQDLIYLILEAQAE
                     VASGNGRRGRPPRESRPAQRAEEGQASSGSATGEKKQRGGRRGRADSRSGHDDKGRAA
                     AERETARGEDRAGRRPETEQRPEDRAGRRPEDRTGRPADGGQRGDDRGGRRPETEQRH
                     DDRGGRRPDGGQRGDDRGGRRPESEQRQEDRGGRRPGEEKRPEEPRRGARIQRVDLRH
                     LHTPGTEDSDRGGERSDSPSRRGRDDRFGRRSRDDRRPQREAEENRPRRESGERGRAD
                     EDQEARPAAQDTRDTGSRESRGDRDARGQRDDRDQAGDRDARGQRDDRDQAGDRDARG
                     QRDDRDQAGDREDREDDGDREGGDDRDDRRGRRGRNRSRENREERRRRVYQDKPVYMQ
                     EYDPDKTPLEGMIRKVGVLQILPDGYGFLRSTEFHYLPSPDDIYVSPSQIKRFALKVG
                     DTVDGEIRPPKEGERYFALIQVNSINGRQPTELEERPTFEHLTPLYPEEALELETSAG
                     EHSTRMLDLFAPIGKGQRALIVAQPKTGKTILLQKIANAITTNHPEIHLLILLVDERP
                     EEVTDMERNVKGEVIASTFDQDPQRHVEVADIVLEKAKRLAEAGQDVVILLDSITRLA
                     RAHNAVAPGGGRTMSGGLEAGALRGPKRFFGAARNIEEGGSLTIIGTALIDTGSRMDD
                     VIFEEFKGTGNSEIVLDRQLADRRIFPAINVVKSGTRREELLIPEMKLGRIWILRKML
                     ADMDPVEAMQFLLDKIRGTRNNDEFVVVMNS"
     gene            complement(9444..10745)
                     /locus_tag="AC16YU_04455"
     CDS             complement(9444..10745)
                     /locus_tag="AC16YU_04455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR6064508.1"
                     /translation="MTRRSDPLEFLDGLPRYATDGAAAVKPGFERVLALLDRLGNPHL
                     AAPTVHVAGTNGKGSTASYIAAIATASGLRSGLHTSPHLVSVTERMRVDGVPAPLAWL
                     SDAVAALRRPILRVGASYFEVTLALSFAWFAEQRADVVVVEVGLGGRLDATNVVDPAV
                     CVITDIGLDHVDLLGDTVQAIAREKAGIMKYGIPVVVADSGPEVEAVLRESARTAGSP
                     LHLVREEVVVTAGVNRLTLETPLHRYENIPVGLAGSVQTRNAALAVRAVELLSGYEGL
                     EGVAWPDAVRAGLTDVTCMSGLRGRMETLLTSPLVVADVSHNAEGIRSALETFGAMHP
                     TGRRMVVLGIMADKPVRTMVRSVVESGALVVPVAVPSDRAVPAAELAAVVRDLGGTCE
                     AVIEEGPGSVPGWIRTHAVAGDGILIAGSHYLVGPVLAAWS"
     gene            complement(10742..>10952)
                     /gene="tsaE"
                     /locus_tag="AC16YU_04460"
     CDS             complement(10742..>10952)
                     /gene="tsaE"
                     /locus_tag="AC16YU_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6064509.1"
                     /translation="LLYHVDAYRVESEAELSELGIEDVLYGEGWCVIEWPERLGRLLP
                     PRAVRIRLEHRGANHRHISLPAVPS"
BASE COUNT         1514 a         3791 c         3789 g         1858 t
ORIGIN      
        1 gaagcggatg cccccgccgc ccgcgggatc gggctcgaag cccgtccggt tcacggacac
       61 tacgaagcag ccgttggcga cggcatgcga gcgctggatg gtctcccacg cgccgtgctg
      121 ggccgcgccg tgcgtgtcgg cttcctcggg gtgccacccg attgcggtcg ggtagaacag
      181 gacttcggcg ccctgcaggg ccgtgagccg tgcggcctcc ggataccact ggtcccagca
      241 gacgagcacg ccgagcgttc cggcgcccgt cggccacgcc aggaacccga ggtcgcccgg
      301 cgtgaagtag tacttctcgt agtaccgggg gtcgtccggg atgtgcattt tccgatactt
      361 gcccacgtat ccgcgctcgc cgtccaggac ggcggccgtg ttgtggtaga gcccgggcgc
      421 ccggcgctcg aagaggctga gcacgaggac ggccccgctc tcccgggcca agggctcgaa
      481 gagatcggtg gtcgggccgg ggaccggctc ggccaggtcg aaatgcgccg ggtcctccga
      541 ctggcagaag tagcggctcc ggaagagctc ggggaggcag acgacctggg cgccaccggc
      601 gacggcctcg cgcgtcaggt cgagggcgcg gcccgtgttc gcgaccgggt cgtccgtcat
      661 cgccatctgc acgagcccta cccggatgcg gcgatcgctc atcgtccgga ccggtcccgg
      721 cgccggtccg ccgccgtgtt gaagtaggtc gactcccgga aggcggcgcc gtactcctcg
      781 agcagacgga cggcggcccg tggacgcagc gtgccgtcct ccaccttgcc gcgcatgatg
      841 ccctccatgc gcttctgcag gtccttcggg aagtactgca cgagggcgag gatctcctcg
      901 acggaggtcg gcggaatcag cttctcgatg tagaagccct ctgcctcgtg gtcgtcggcg
      961 tagacgtgca cctcggccac gcggccgaac aggttgtgca tgtcacccat gatgtcctgg
     1021 tacgcgccca tcaggaagaa acccagcagg tacggctcgc cgtcacgcaa tgcgtgcacg
     1081 tccaggaacc gcttgtcgtc gttggccgaa acgtagcggc ggaccttgcc gtccgaatcg
     1141 cacgtgaggt ccacgagcac ggcgcgacgg tcgggctcct cgtccaggcg ctcgatgggc
     1201 acgatgggaa accgctgccc gaacgaccag tggtccagga tggactggaa gacggagaag
     1261 tcgcacaggt actggtccac caggtgctcg tcgagggcct cgagctcggc cggcagccag
     1321 tcggcattcg cgccgcgcac gcggttgttc agcgactggc agatggtcca gtacagttgc
     1381 tcggccaggg ccttctgctc gatggacagg tagccgaagc cgaagagcga ctcggcctcc
     1441 tgacgcttgg cggccgcgtc gtgataggcc tcgagcaggg cgcccgggcg gagctgccgg
     1501 tcctcgcgga tccaggagag ggtctcgaag agctgcttta cgacggcgtg gtcctcgtcc
     1561 gccggctcga atccgtccgg gatctcgtcc ttgcggtagg cgccgagcac ctcgacgacg
     1621 aggacggagt ggtgggcggt gagcgcgcgc ccgctctcgg tgatgagggt cggctgcgcg
     1681 actccctccg agtcgcatac ctccttgacc tgcgcgacca cgctgttcgc gtactcctgc
     1741 agcgtgtagt tgatgccctc gctcgtgccg cccgtgtagt cggcctcgta gttcacgccc
     1801 agcccgccgc ccacgtccag gaagcggatg ggcacgtcgc gcttcacgag ctgcgcgtag
     1861 atctgcgtga tctccttgat gcccttgcgg agcgtctgga tgtcgttcac ctggctgccc
     1921 aggtggaaat gcaggagcac gaaggcctcg tcgagctcgt cctcctcgag gcgcgtgaga
     1981 agccggagta gctcgggaat cgagatcccg aatttcgaca ggtcgccgcc cgagtcggcc
     2041 cacttgccgg atccgcccgt gttcagccgg acgcgaaccc cgaaacgggg cttgatccgg
     2101 cgcttggcgg ccaggcgcag caggcgctcg aactcgtcgt acttctcgag cacggggatg
     2161 acctgcttgc cgagctgctg tcccgtgagg atcagctcca tcatcacgga gtccttgtac
     2221 ccgttgacga tcaggagcgt gtcgtcgtcg gtcaggtggg gaagcgtcgc gacgagctcg
     2281 gccttggagc cgcactcgag gccgatgccg aacggccggc cggcctcgag gatctcctcg
     2341 acgacctcgt gaagctggtt caccttgatc gggtaaacgc cgcggtagcg ggcctcgtac
     2401 tcgaactcgg cgcgggcggc ctcgaactcg gcgttcagct gctcgacgcg ccctcggatg
     2461 atgtcctgga agcggatgag gaccggaaag ggcaccccgt cctcgcgaag ctggctgacc
     2521 acctcgctga tcgcgatggc cgcggagccg tcgcgacgca cgtcggcgac catgcgtccc
     2581 cggtcgtcca cgcggaagta tccgtcactc cacccctgga tatggtaccg cgcctgcgcg
     2641 tccgagggct gccagccggc ctcccgaggc gcctccgtat ccgtcacgac cccgacttcg
     2701 ctcatctatc cacctccgaa tccacgccgc gtccggccag gcgggcggcc tcggcggccg
     2761 cggccctgtc cgactcgagc acgtaggcaa aaacgagcgg cgcgacgatc gtcgcgtccg
     2821 actcgatgat gtaggacggc gaatcgatcg agagcttgcc ccaggtgatc ttctcgttgg
     2881 gcacggctcc cgagtacgat ccgtaagagg tggttgaatc gctgatctgg cagaagtatc
     2941 cccacttcgg cgtatttcgc cccaggtcct gttccagcat cgggactacg cagatcggga
     3001 agtcgcccgc gatgccgccg ccgatctgga aaaacccgat cgaatggccg gccgactccc
     3061 gttcgtacca ctcggcgagg accatcatgt actcgatgcc cgagcggacc gtgcgcggat
     3121 cgcgcacgtc gcccttgatg cagtggccgg cgaagatgtt gccgagcgtc gagtcctccc
     3181 agcccggcac cacgatcggg agattccgct cggccgcggc cacgagccag gagtccgccg
     3241 ggtcgacctg gtacgaggcg gacagcacgc cgctgaggac gagctcgtac aggaactcgt
     3301 gcgggaagcg ccgctcgccc gagcggtcgg cggcctgcca caggtccagg acctggcgct
     3361 cgatccggcg gatcgcctcc tcctccggga tgcacgtgtc ggtgacccgg ttcaggtggc
     3421 gctcgagcag ggcctgctcg tcctcggggc gcaggtcgcg gtaccgcggc acccgcacgt
     3481 aatggtcgtg cgcgaccagg ttgaagagat cctcctccag gttggcgccc gtgcagctga
     3541 tggcgtgcac cttgccggcc cggatcatgc cggcgagcga gagccccagc tcggccgtgc
     3601 tcatggcgcc ggcgagcgtg accatcatgc gcccgccgcc ctccaggtgg tccacgtagc
     3661 cctgagccgc atccaccagg gccgccgcgt tgaaatgccg ataatgatgt cgaatgaagt
     3721 ccgtgatggt catgatctat cggttttcac gcggcccggg gccgcatcag tccagcgcgt
     3781 cgtcatcctc ctcgcccagc ccgagcccgc ttccccgccg gttgcggtca cgcggccggc
     3841 tctccctgcg gtcgcgggga aggccccggc ccgggcgcgg gatgcggggc ctgggttcgt
     3901 ccaggtcctc ggtgagcccg cggaaacggg tctcgaggag gggctcgtcg tcccagtcgt
     3961 cgtccccgcc ctcgatccag tcgccgccct ccgcgtcctc atcgacgtct tcgacttcgt
     4021 cttcgtcttc gtcttcgtcc cagtcgtcct cgtcttcgtc ctcgtcctcg tcccagtcgt
     4081 cctcgtcctc gtcatcctcc tcccaatcct catcctcgtc caggtccccg atctcgtcat
     4141 cctcgtccgg ttcggcgtca tcgtccgcct cgggctcttc gtccggatcg agatccagga
     4201 tctgcggcaa gggaaacggg gtttcgggga ccactcccgt ctgcgttacg gtatccatgg
     4261 cggtatgcgg tatggacaaa cgaaccccgg ccccggtacg actgcgtcca cgctccatcg
     4321 tggcgactgg ccgacggccg ggaaggcact atgtgtcggt ggcgtctggg gtgggcgccc
     4381 gggggtgacc ggtgcgccgg gtgtcgcgcg ccgcgcgaac attgaggccg gctgatgatc
     4441 tggttcgggt cccgtcgcgg gaccgcaaca gttcaatata tacgaaacgg cgcccaaacg
     4501 gtgcataacc cccgcggttt ccggccgggt ggacgggaat cgggttccgg ctagcggagg
     4561 atcggtccgg acggacggaa ttcaagcggc gaacggtgcc cacgaaggga gaggggcccc
     4621 ggaccgtccg gcctttacga cgacgggcgg tgggtgttga gaggtgcacg gttctcggcg
     4681 gaccatggtg gccccgtcgt gttatcgcag tgttcaggga cgattgcctg tgtgtggtcc
     4741 gtggaccgac acttcggcgg cggtcgcccg tacacgcgcc ccctcgccgc ccgcggagcc
     4801 cgtgcggagg cccgccggag gcctccggaa taggaacacg ggctacagcc acctgttgga
     4861 acgcaatccg ctaccccgga tcgtgaagcg cgccccaaaa agcgactacc tcgttctctt
     4921 cccgctctgg ctgatggtct tctcggccag cagccaggtg atcatcatct cgccgatcct
     4981 cccgcgcatc ggcgaggcgc tgatggttcc ggacgagcac ctgggctggc tgatcacggc
     5041 ctatgcgacc ttcttgagcg tgttcgccct gatcatcggg ccgatctccg accgcgtggg
     5101 ccgtcgtcgc gtgctgctca tcggttcggc gtcgatgact ttcgccctga tgctgcacgg
     5161 cctggcggac agtttcacga gcctgctcgt ggtccggtcg gtcgccggcg cggccggggg
     5221 catgctctcg ggcggcgccg tctcgtacgt cggggactac ttcccgtacg agcgccgggg
     5281 ctgggcgaac gggtgggtca tgagcggcat cgcggtcggg cagatcatcg ggattccgat
     5341 aggcacgctg atcgcggcca gtatgggttt ccgggccgcc ttcatgctct tcgcgatcac
     5401 gatgggcttt gcgaccctcc tgatcgcgct cttcgtcccg cagccggacg tggcgcgcag
     5461 caccgggcgg ctgaccgtcg tcggctcgct ccgcgcgtac tggcagctgc tgcgcaggcc
     5521 cgtcatcgtc gcggccacga tcgtgtactt cctgatgttt ttcagcctcg ggctgtacgt
     5581 catctatctg cccacctgga tcgagcggtc gctctcggtg cagggcacgg ccatcgcgtc
     5641 cctgttcctg gtgggcggca tcgccaacgt catctcgggg ccggtcgccg gacgcatctc
     5701 cgactcgatc ggacgcaagc ccgtgatcgt cgcctcgtgc gtggggctcg cgatcgtcat
     5761 gatcgcgacc acgcacctgg tccattccat gggcatggcc tacatcgtgt tcgcgctggc
     5821 catggccatg gtggccatgc gggtgagccc gctgcagtcc ctcatgacgg agctcgtgac
     5881 ggccgaccgg cggggagccc tgatgtgcct ggccgtcgcg ctcggccagt tcggcatcgg
     5941 aatcggcggg gccgcggcgg gtcccgcgta cacgcatttc ggatacgcca gcacgaccta
     6001 cgccggcgcc gtggccatcg cgctgatggc gttcatggtg cagcggttcc tgcccgagcc
     6061 gtcggcgcac taccggccgg tgggccggcg ggtcgacggt cagtccggaa gcgaggagac
     6121 cgcgtcggcc aaggcgtcgt gatcgacgat caccgcgtgg gcgccgcgat cccgaaggag
     6181 ggacgcgtga cgcccggcgt ccaggtaggg agggacgaac ccgatggcgc ggcatccggc
     6241 ggcccgcgcg gcggtcatgt cgtcgaccga gtctcccacg tagaccgctt caccgggatc
     6301 gaccgcgcgc ccgaggctcg ccaggcgctc gaaggcgatc tgcagcggcc ttggatcggg
     6361 cttgccgcgg cctacggcgt cttccatcgt gaccagcacg tcgaaccggt ccgtccagcc
     6421 gaaccggtcc agggtgaagc gcgcttcctc gcggggccgc cccgtgacca gccccaggga
     6481 gccgaaccgg tcccgcagcg cgtcgagctg acgggccgtc acgagcgccg gctccgcggc
     6541 gatgagtccg tcccagtccg ggccccgata cctcaggttg aactcgcgga ccacgtcggc
     6601 gaacgcgacg cgcgtgcccg tccgggcgat gagctccgcc gtcacgcgcc agtcgtcgtt
     6661 gtagccgccc gaatccttca ggcgctgcac ggccgcgtcg cccacgggga ttcccgtgaa
     6721 gtgctgtgcc gtcgccctga tcgccagtcg gtacgagccg gacacgtcga ccagcacgcc
     6781 gtcgagatcg aacagagcgg cccggatcgt cgccatcgag cgtcggtcag gagttcatca
     6841 cgaccacgaa ctcgtcgttg ttgcgcgtgc cgcggatctt gtcgagcagg aactgcatgg
     6901 cctccacggg atccatgtcg gccagcatct tccgcaggat ccagatccgg ccgagcttca
     6961 tttcggggat caggagctcc tcgcgccgcg tgccggactt gaccacgttt atggccggga
     7021 agatgcgccg gtccgccagc tggcggtcca ggactatttc cgagttgccg gtgcccttga
     7081 actcctcgaa gatgacgtcg tccatgcggc tgcccgtgtc gatgagggcc gtgccgatga
     7141 tcgtcagcga accgccttcc tcgatgttgc gggcggcgcc gaagaagcgc ttcgggccgc
     7201 gaagtgcgcc ggcttcgagg ccgcccgaca tggtccgtcc gcctccggga gccacggcgt
     7261 tgtgcgcgcg ggccaggcgc gtgatggagt cgagcagaat gaccacgtcc tggcccgcct
     7321 cggccagcct cttcgccttc tcgagcacga tgtcggcgac ctcgacgtgc cgctgcggat
     7381 cctggtcgaa ggtcgacgcg atgacctcgc ccttcacgtt ccgctccatg tcggtcacct
     7441 cctccggacg ctcgtccacg agcaggatca ggagatggat ctcgggatgg ttcgtggtga
     7501 tcgcgttcgc gatcttctgg agcaggatcg tcttgccggt cttgggctgc gccacgatga
     7561 gggcgcgctg gcccttgccg atgggtgcga agaggtccag catgcgggtc gaatgctcgc
     7621 ccgccgacgt ctccagctcg agagcctcct cgggatacag gggcgtgagg tgctcgaacg
     7681 tgggccgctc ctcgagctcg gtcggctggc ggccgttgat cgagtttacc tggatcaggg
     7741 cgaagtagcg ctcgccttcc ttgggcgggc ggatctcgcc gtccacggtg tcgcccacct
     7801 tcagcgcgaa gcgcttgatc tgggaaggcg acacgtagat gtcgtcgggg ctgggcaggt
     7861 aatggaattc cgtcgaccgg aggaagccgt agccgtcggg caggatctgc aggacgccga
     7921 ccttccggat catcccctcg aggggcgtct tgtccgggtc gtattcctgc atgtacacgg
     7981 gcttgtcctg gtacacccgg cgccgccgct cctcgcggtt ctcgcggctg cggttgcgcc
     8041 cgcggcgccc ccggcgatcg tcgcggtcgt caccaccctc gcggtctccg tcgtcctcac
     8101 ggtcctcgcg atcgccggcc tggtcgcggt cgtcccgctg gccccgcgcg tcgcgatcgc
     8161 cggcctggtc acggtcgtcc cgctgccctc gcgcgtcacg atcgccggcc tggtcgcggt
     8221 cgtcccgctg gcctcgcgcg tcacgatcgc cgcggctctc ccgggagccg gtgtcccggg
     8281 tgtcctgggc ggccggccgg gcttcctggt cctcgtcggc ccggccgcgc tctccggatt
     8341 ccctgcgggg ccggttctcc tcggcctccc gctgcggcct tcgatcgtcg cgggagcggc
     8401 ggccgaagcg gtcgtcccga ccgcggcgcg acgggctgtc ggagcgctcg cccccccgat
     8461 cggaatcttc ggttccgggg gtgtgcagat gcctcaggtc cacgcgctgg atgcgcgctc
     8521 cgcggcgggg ctcctccggg cgcttctctt cccccgggcg tcgtcccccg cggtcctcct
     8581 gtctctgttc gctctcgggg cgccgtcccc cgcggtcgtc gccgcgctgt ccgccgtcgg
     8641 ggcgccgtcc tccgcggtcg tcgtgtcgct gttcggtctc ggggcgtctt cctccgcggt
     8701 cgtcgccgcg ctgtccgccg tcggcgggac gaccggtgcg gtcttcgggg cgccggcctg
     8761 cgcggtcttc ggggcgctgc tcggtttcgg ggcgccggcc cgcgcggtct tcgccgcgag
     8821 cggtctcgcg ctcggcggct gcccttccct tgtcatcgtg cccggaacgg gaatcggccc
     8881 ggccgcgacg cccgccacgc tgcttcttct cgcccgtcgc cgagccggag gacgcctggc
     8941 cctcctcggc ccgctgcgcc ggccgcgatt cgcgcggcgg acgtccgcgt cgtccgtttc
     9001 cggaggcgac ctcggcctgg gcttcgagaa tgagatagat caggtcctgc ttgcgcaggg
     9061 tggagaaccc cgacaggttc atctggcggg caatctcctt gaggtcggaa atcgtctttg
     9121 cctggaggtc tgcaattcgc atgacggaaa cggatacggc tggagcccgg ccccgggaag
     9181 aaagcgtggg tggcgtgata cccgggcggc cggtgattgc ggcgtcgttc ggacgcagtg
     9241 gaccggacgg tccagtggaa agggttcaat cggcggacat ggcaacggcc tcttcggccg
     9301 gcccgtgcgg tgcgatcccg gaaaggagat ggggatcgaa gggtcctgtc gcgcctggac
     9361 ggcgagacag gcttcgaaga tagtgctgca acgcaagaaa tccacgttcg ttctcccggg
     9421 cggacggggg cccagcgcgg cgctcaggac caggcggcga gcaccggacc gaccaggtaa
     9481 tgggagcccg caatgaggat gccgtcgccc gccacggcat gcgtgcggat ccagcccggt
     9541 acgctcccgg gcccctcctc gatcacggcc tcgcacgtgc cgccgaggtc ccggaccacc
     9601 gcggccagct ccgcggccgg caccgcccgg tccgagggca ccgcgaccgg cacgaccagc
     9661 gcgcccgatt cgaccacgct ccggaccatc gtccggacag gcttgtccgc catgattccc
     9721 aggacgacca tgcgtcggcc ggtcggatgc atggccccga aggtctcgag cgcggaccgg
     9781 atgccctcgg cgttgtgcga gacgtcggcg accacgaggg gcgaggtcag cagcgtttcc
     9841 atcctgcccc ggagaccgct catgcacgtg acgtcggtga gccccgcccg gacggcgtcg
     9901 ggccaggcga cgccctcgag accctcgtac ccggaaagga gctccaccgc ccgcacggcg
     9961 agcgcggcat tgcgcgtctg gacggagccc gcgagtccga cggggatgtt ctcgtaccgg
    10021 tgcagcgggg tttcgagcgt gagacggttc acgccggcgg tgaccacgac ctcttcccgg
    10081 accaggtgga gcgggctgcc ggcggttcgg gccgactcgc ggagcacggc ctcgacctcc
    10141 ggcccggagt ccgccactac gacggggatt ccgtacttca ttatgcccgc cttttcgcgg
    10201 gcgatggcct gcacggtgtc cccgagcaga tccacgtggt ccaggccgat gtcggtgatc
    10261 acgcacacgg ccggatcgac gacgttcgtg gcgtcgagcc ggccgccgag gccgacctcg
    10321 acgacgacga cgtcggcccg ttgttcggcg aaccaggcga aagagagggc gagcgtgacc
    10381 tcgaagtagc tggcgcccac cctgaggatc ggccggcgca gtgcggcgac ggcgtcggac
    10441 agccaggcga ggggtgcggg caccccgtcc acccgcatcc gctccgtcac ggatacgagg
    10501 tgcggcgagg tgtgcaggcc ggagcggagc ccggaggccg tcgcgatcgc cgcgatgtac
    10561 gaggccgtcg atcccttgcc gttcgtcccg gcgacgtgca cggtgggagc ggcgaggtgc
    10621 ggattgccca ggcggtccag caatgccagg acccgctcga accccggctt gacggcggcg
    10681 gcgccgtccg tggcgtaccg gggcagcccg tccaggaatt cgagtggatc ggatcggcgg
    10741 gtcacgacgg tacggccggc aggctgatgt gtcggtggtt ggccccccgg tgctcgagcc
    10801 gaatgcggac ggcgcgcggc gggagcagcc ggcccagtcg ctccggccac tcgatcacgc
    10861 accacccctc gccgtagagc acgtcctcga tgcccagctc ggacagctcg gcctcggatt
    10921 ccacgcggta cgcgtccacg tggtaaagga gc
//
LOCUS       DBNHQZ010000171        13044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3711262, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000171 DBNHQZ010000000
VERSION     DBNHQZ010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13044
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3711262"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC16YU_04465"
     CDS             <1..300
                     /locus_tag="AC16YU_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR6064510.1"
                     /translation="NALTSNQYATSFIVGDAFQERFNELAIPRRVLSRSIEDYGTMIE
                     PLVPWHPTALFMVATLGVPVSAYWNWELLTLANLVVAPVLAITGIGCFYGKPKPD"
     gene            384..1571
                     /locus_tag="AC16YU_04470"
     CDS             384..1571
                     /locus_tag="AC16YU_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6064511.1"
                     /translation="MHSDHLETRLAHVGEDRASHSGAVVPPIYQNSLFTFESWDAIDR
                     AFDDRVEHPIYTRGTNPTVRAVERKLASMAGAPAARLFASGMAAISAAVMHSVGPGDH
                     VVTIRNVYGPTQNLLGRYLRGKMNLQTTFVDGTDPADFERAVTERTRLFYLESPSSAV
                     FSLQDLAAVARIARARGIRTIADNTWATPLFQKPLELGIDLEVHSVSKYLGGHSDLVA
                     GALLGSEEDIRSIAVTEVELLGGIMAPFEAWLLQRSLRTLPMRLERHQANAAVVARFL
                     ESHPGVSRVRWPGLESHPQAELARRQMTGFSGLMGFTLATDDLPAIKRFFDALRLFQR
                     GVSWGGHESLVYAPAISYLKELPPERFAELGISLGDMRISVGLEDPGDLVQDLDRALA
                     LVA"
     gene            complement(1568..3622)
                     /locus_tag="AC16YU_04475"
     CDS             complement(1568..3622)
                     /locus_tag="AC16YU_04475"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF036527.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HIR6064512.1"
                     /translation="MSDTKTLIRRDLARLAFSARLMQEAEDPSVPLYERLHMLARATC
                     VLDALVLERGADLVPGARPGRPGRRFRTVRREADALRRRIGTAYRSQLLPALERRGIR
                     ILVRDELDSLQRSWVRTCFEHEVVEHLDVRLVDGPDAVRGLQPGCYLAVSRRDATPAA
                     IGAPVRPFGLVSIPRLSMGRFAALPEVGGERPVIFLDDVIRANLDRLFPEFEPEQGFA
                     IWLASAGEAARLGETAGQAPTGIPGSGTRLVHDRRMPFPLLATIRDGLGLAEDDLVPG
                     GRYPALGDLVGFPRRGLESTVRSAPPPLARSDLEGSLFDAVAGGDRLLRFPFESFEYL
                     LRFLREAADDPDVRSIRALLSGPGGAVAGVLGEAARAGRSVTAVVGHPGQDGAEALAD
                     EGVRVVRARDGLDVASDLVLVERDEVDGPRAYACLSTGRLDPEAALRSVDYALLTADR
                     RITKDTGRLLDHLEGRRRQPRLEHLLVAPRGMKRRLKWLLRTEELYREADRPAGVLLN
                     LRALEHGGMARRLARAALRGVPIAGVVYGACRVPSRPGLVMRSIVDRYVGNAGIWWFT
                     NGGDPACYLSSVDWTRQGLGGGIGIAFPVFSVELRAEIRADLDRRLSDNVKARIIDSG
                     RTNPFWRGGFGAVRSEDASYRALADRRAIVSARGPAAGEAPDEAETEQPGAEMQDVQP
                     VL"
     gene            3813..4706
                     /locus_tag="AC16YU_04480"
     CDS             3813..4706
                     /locus_tag="AC16YU_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064513.1"
                     /translation="MRLLPVLFVAAVLLSSCRARSEYEITFSTDRDESLDIFGMTPDS
                     SYFRGYTGDDGTEYGPRWSPDGSAILYTAWRGGNADIVAFEPERGRIRRLVQAPGYDG
                     NPDWSSDGAHIVFVSDRDGERKLYRTRVDTLDVVRLSGPEGVDTSPRWSPRGNLIVFT
                     RSEPGTTDRGAVMVMNADGTGQRKLTSGDSFDSLADWSPDGSRIAFHRCDDGSCSLWT
                     MSVDGTNENPLLDDGHDNRWPAWSPDGEWIAFTSTRDGQSDIYVVRPDGSDVRRVTRW
                     PGRDEGPAWRPVPRLPEAARQ"
     gene            complement(4776..5861)
                     /locus_tag="AC16YU_04485"
     CDS             complement(4776..5861)
                     /locus_tag="AC16YU_04485"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046444 - FMN metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase family protein"
                     /protein_id="HIR6064514.1"
                     /translation="MTHVFVSGCYDILHAGHLQFFREARALGDRLTVSFASSDVLVRY
                     KGRRGSLPDEHKRALLVALEMVDDVVVGTGADPGLDFIEAFERMRPDILAVTEDDGFE
                     AEKRALCARTGARYVVLPKTPPRFDPVSTSELIRWIRAPREAPLRIDFGGGWLDVPRF
                     ARDGAFIVNCAISPLVSLTDWPYRHRSGLGGSGAWALLNGREGVEAELDLGVGWQDPA
                     VIRETGLCVWRSGSRPSLELKTDGSMLAGRMALAWTGRPHDTPGVVDRPRDFDAIERA
                     GKRARDAAWERDPGGLAEAVRISYAMQVGEGMQPLDEAPGMLACKYAGGGYGGYAAYL
                     FDSDRSRDAFVAGYPDRLPVEPYLRIA"
     gene            5971..6966
                     /locus_tag="AC16YU_04490"
     CDS             5971..6966
                     /locus_tag="AC16YU_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR6064515.1"
                     /translation="MRAALFAVVAAFAATAPADAQPVSIDEWEVPYAESRPRDPYVAP
                     DGRVWFCGQRTGYLAVLDPATGEYRKYDLGEGAGPHNLIVDDSGMVWYAGNRRAHIGR
                     LDPATGNVTKFPMPDDAARDPHTLAWDGEGDIWFTVQGGNFVGHLDTGSGEVRLVAVP
                     TERARPYGIRVDPDGRPWIALFGTHKLATVDPATMELTEVPLSRESARPRRVEISSDG
                     RVWYVDYADGMLGAYDPRDGSIEEWAIPGGPDSRPYGTALDERDRIWFVETGPQPNRL
                     AGFDTRAATWLDATPIESGAGSVRHMYFHPPTRTIWFGTDANTIGRARLADDPGR"
     gene            6950..7483
                     /locus_tag="AC16YU_04495"
     CDS             6950..7483
                     /locus_tag="AC16YU_04495"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HIR6064516.1"
                     /translation="MTRAGSLSLLLPVLVLGLPHPDGPPPAHTGGFGEPTCTDCHADF
                     EVNEGPGGLVLTGFPERISPGAVYDLKVFLRHEGMARAGFQLSARTPDGAQAGRLAPV
                     SDRTATATEDGIEYAGHTEAGSFAAGDSAAWTVRWTAPDSIAGDLVVHVTANAANGDD
                     SPFEDWVYAGAWTLGIH"
     gene            7511..8623
                     /locus_tag="AC16YU_04500"
     CDS             7511..8623
                     /locus_tag="AC16YU_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064517.1"
                     /translation="MRHLASLSLVVLLTATGARAQVVDSTRTDDDLERELERELAAPA
                     PPRQRSGTNPDISVIGDTRAWTVSEGERNVDLGVHEVETAFRSTVDPYARADVYLSVG
                     SEDGEFEFELEEAYLTTLSLPAGLQLKAGKFRSAFGKINRTHPHALPFIDVPAPYANF
                     LGDEGLNDQGISVNWLVPNARFYQEVSFEVTRGPAESASFETDDGNRLLYAGRFMNFW
                     DLSPDATLEVGVSAASGPNPSGRTSELVGLDVTYRWKPLRYNTYRSFLLQAEGLLSRN
                     RTDLGTVDGFGFYALAGYQLGRRWSVFGRFDHADLPDDADWNEYGISGTLGWYLTEFQ
                     KLEFGLRHAWADGMDPVWQGLVRLVFVIGTHGAHEY"
     gene            8682..9635
                     /locus_tag="AC16YU_04505"
     CDS             8682..9635
                     /locus_tag="AC16YU_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR6064518.1"
                     /translation="MRRLSPYIQGLARHLAALLFAVCLGTTASAQPKLQVVATLTDLK
                     SIAEEIGGDRVEAFAIATGFQNPHFVDPKPSYIVRLARADLFVTVGLDLETGWVPPLL
                     NSARNPRILPGASGYVDASANVPLLQVPSSVSREQGDIHVYGNPHYWLDPERGKIVAR
                     NIADGLKRLEPGSADYFEENLERFTAAIDQKMLEWTVRLAPYAGTPVIAYHNEYPYLE
                     SAFGFRIVDFLEPKPGIPPSPAQLARVIRTMKDRGIRVIIISPYFNPDAAELVAGKIA
                     GGTVVTIASSVEAFHGIETYFDLFDYNVDQLVRAFQQTPGP"
     gene            9644..10483
                     /locus_tag="AC16YU_04510"
     CDS             9644..10483
                     /locus_tag="AC16YU_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR6064519.1"
                     /translation="MSDLFALDFVRNALLVSVIMGLMLSYLGVHVVGRGIVFVDLALG
                     QISMLGVAVATYFETDATTVSVVFTLVGAILLSFIKTTDRRLKQEAIIGIIYAVSSAV
                     TVLLISKAAHGDADIQEVLFGSVFTVTPAQIGWMAGVFGVLALVHVFFRRQLFEVTRK
                     YEAKEVQDLGAFNLWNFVFYVSIGLAIVLAVRVGGVIPVFAYLVVPPVAGILMTTRSH
                     AGVVLIALPISVVGSLAGVYFSVQFDFPAGPSVVAMLGAIFAVAAIVRLFRGRVAPGP
                     AEA"
     gene            complement(10480..12072)
                     /locus_tag="AC16YU_04515"
     CDS             complement(10480..12072)
                     /locus_tag="AC16YU_04515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6064520.1"
                     /translation="MRAFSLLLLVLLVTATRPVAARQDPGAPAGEGPFDRLVIRGGIM
                     IDGAGAPPVGPVDIVIEGDRIAEIRTVGYPGLPIDSTRRPAPGTREIDAHGQYVLPGF
                     IDMHGHTHGEGSGQGVSAEYVRKLWLAHGITTVRELAGGDVDAMLEARRLSAENRIAA
                     PRYFLYPVFGAGADAGTDTPDAAREWVRWVAGRGVDGIKFFGAPPEVMRAALDEMRAR
                     GLRGTEHHAQLDVTRMNVLRTARAGLTSMEHWYGLPEALFEDRVIQQYRLDYNYQDES
                     HRFGEAGRLWKQAAAPFSRHWNAVMDSLLALDFTLDPTFVSYLASRDLEAQSRRSFHA
                     EYTLPSLWRYYRPSRAAHGSYWFYWTSENESDWKENYRLWMAFVNEYKNRGGRVTLGS
                     DSGYIYNLYGFGYIQEMELLREAGFHPLEVIRSATIQGAEALGADRDIGSIHVGKQAD
                     LVIVPENPLENLKVLYGNGTLRLNDATGEVERVGGVRWTIRGGVVFDAPRLLEDVRAM
                     VREAKAAEGIPDGPMPIETVRR"
     gene            12156..>13044
                     /locus_tag="AC16YU_04520"
     CDS             12156..>13044
                     /locus_tag="AC16YU_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR6064521.1"
                     /translation="MQTRTKKAPDGSVPGPAVSRGTLVLALALLLAPARPSGAQPVVI
                     SELQADNATTVADPDFGAFADWIELHNAGTAAVDVGGYFLSDDPDLPGRWRIPDGTAI
                     APGAYLVVWADGLDTGLHASFALGRDGETVRLSDAAGVPLDEVVFGPMPSDVSFGRRP
                     ADPSTWGYLATPTPGAANAGPAYDGFLDPPAFDPPGGFFAVPVQVTAASGDPAAVVRF
                     TTDGTAPHDASPAADGPIEVAANTVLRAAAFRPGHPASPVTTATFFVGESPTLPVVSL
                     ATDPDNFFDDEIGIYVAGTN"
BASE COUNT         1907 a         4811 c         4386 g         1940 t
ORIGIN      
        1 aatgcgctca cctcgaacca gtacgcgacc agcttcatcg tcggcgacgc gttccaggag
       61 cggttcaacg agctcgccat cccgcggcgc gtgctctcgc gatcgatcga ggactacggc
      121 acgatgatcg agcccctcgt gccgtggcat ccgacggcgc tcttcatggt cgccacgctc
      181 ggcgttcccg tctcggccta ctggaactgg gagctcctga cgctcgccaa cctcgtcgtc
      241 gctcccgtcc tggcgatcac ggggatcggg tgcttctacg gcaagccgaa gccggactga
      301 ggcgcccggc tacggaacgc gccgctcgcc cggccgccgc cgggacgtac ctttgcgtcc
      361 ctgccaaccc tccagcggcg cccatgcatt ccgaccacct cgaaacccgc ctggcccacg
      421 tcggcgagga ccgcgcgtcg cactcgggcg ccgtcgtgcc gccgatctac cagaactcgc
      481 tcttcacgtt cgagagctgg gacgcgatcg accgggcctt cgacgaccgg gtcgagcatc
      541 cgatctacac gcgcggcacg aatcccaccg tccgcgcggt ggagcggaaa ctcgcgtcca
      601 tggcgggcgc cccggccgcg cgcctgttcg cgtcgggcat ggccgcgatc tcggcggccg
      661 tcatgcactc ggtgggcccg ggcgaccacg tggtgacaat acggaacgtg tacggtccca
      721 cgcagaacct gctcggccgc tacctgcgag ggaagatgaa cctgcagacg acgttcgtcg
      781 acggcacgga tcccgccgac ttcgagcggg ccgtcacgga gcggacccgg ctgttctatc
      841 tcgagagccc gtcgtcggcg gtcttctcgc tgcaggacct ggccgcggtc gcacggatcg
      901 cccgggcgcg cggcattcgt acgatcgccg acaacacctg ggccacgccc ctcttccaga
      961 agcccctgga actcgggatc gacctcgagg tgcactccgt ctcgaagtac ctgggcggcc
     1021 acagcgacct ggtcgccggc gcgctgctcg gcagcgagga ggacatccgg tcgatcgccg
     1081 tcacggaggt cgagctcctg ggagggatca tggcgccctt cgaggcctgg ctgctgcagc
     1141 gcagcctccg gaccctgccc atgcgcttgg agcggcacca ggcgaacgcg gccgtggtcg
     1201 cgcgcttcct cgagtcgcac cccggggtct cgagggtgcg ctggcccggc ctggaatccc
     1261 atccccaggc cgagctcgcc cggcgccaga tgacgggctt cagcgggctc atgggattca
     1321 cgctcgcgac cgacgatctc ccggcgatca agcgcttctt cgacgcgctc cgcctgttcc
     1381 agcggggcgt gagctggggc ggccacgaga gcctcgtcta cgccccggcc atatcgtacc
     1441 tgaaggagct ccctccggag cggttcgcgg agctcggcat ctcgctcggc gacatgcgga
     1501 tatcggtcgg actggaggat cccggggacc tcgtgcagga cctggaccgg gccctcgcgc
     1561 ttgtcgccta gagcaccggc tgcacgtcct gcatttccgc cccgggctgc tcggtctctg
     1621 cctcgtcggg cgcctcgccg gcggcgggcc cgcgcgccga gacgatcgcg cggcgatccg
     1681 cgagcgcgcg gtacgacgcg tcctccgacc gcacggcgcc gaagcctccc cgccagaagg
     1741 gattcgtccg gccgctgtcg atgatccggg ccttcacgtt gtcggacagc cgccggtcca
     1801 ggtcggcacg gatctccgcg cgaagctcca cgctgaagac cgggaaggcg atgccgatgc
     1861 ccccgccaag gccctgccgg gtccagtcga ccgacgatag atagcacgcc gggtcgcccc
     1921 cgttcgtgaa ccaccagatg cccgcgttgc ccacgtagcg gtcgacgatg ctgcgcatca
     1981 ccaggcccgg acgcgacggc acgcggcagg cgccgtagac cacgccggca atgggcacgc
     2041 cccggagggc ggcacgcgcc aggcggcggg ccattccgcc gtgctcgagg gcccgcaggt
     2101 tcagcagcac gccggcaggc ctgtcggcct cccggtagag ttcctccgtg cggagcagcc
     2161 acttcaggcg gcgcttcatc ccgcgcggcg ccacgagcag gtgctcgagc cgtggctgcc
     2221 tgcgccgccc ctcgagatgg tccaggaggc gccccgtgtc cttcgtgatc cgccggtcgg
     2281 cggtcaggag ggcatagtcg acgctgcgca gcgcggcttc aggatccagc ctcccggtcg
     2341 agaggcacgc gtacgcgcgc gggccgtcca cctcgtcccg ctccaccagg acgagatccg
     2401 acgccacgtc gagcccgtcg cgcgcgcgga ccacgcgcac gccctcgtcc gccagcgcct
     2461 ccgctccgtc ctgccccggg tgcccgacca cggccgtcac gctccggccc gcccgggcgg
     2521 cctcgcccag cacgccggcg acggctcctc caggccccga aagcagggcc cggatgctcc
     2581 ggacatcggg atcgtccgcc gcctcgcgca gaaagcggag caggtactcg aaggactcga
     2641 aaggaaagcg gagcaggcgg tctccgcccg cgacggcatc gaacaggctg ccctccaggt
     2701 ccgaccgggc gaggggcggc ggggccgatc ggaccgtgga ttcgagcccc ctgcgcggaa
     2761 acccgacgag gtcccccaat gcggggtagc gccctcccgg aaccaggtcg tcctcggcga
     2821 gcccgagccc gtcgcggatc gtcgcgagca gcgggaacgg catccggcgg tcgtggacca
     2881 gccgcgtacc gcttccgggg atcccggtgg gtgcctgccc ggccgtctcc cccaggcgcg
     2941 cggcttcccc ggcggaggcg agccagatcg cgaagccctg ttcgggctcg aattcgggga
     3001 aaagccggtc caggttggcg cggatgacgt cgtccaggaa gatcaccggc cgctcgccgc
     3061 ccacttcggg aagcgccgcg aaccgcccca tcgagaggcg gggaatcgac acgagcccga
     3121 atggccggac gggggcgccg atggcggcgg gcgtcgcatc ccggcggctc acggccaggt
     3181 agcagccggg ctgcagtccc cgcacggcgt ccggtccgtc gacgagccgc acgtccagat
     3241 gctcgacgac ctcgtgctcg aaacaggtcc gcacccagct ccgctgcagc gaatcgagct
     3301 cgtcgcggac caggatccgt atgccgcgcc gctcgagcgc tggcagcaac tgggaccggt
     3361 acgccgtccc gatccgcctg cggagcgcgt ccgcctccct ccgcaccgta cggaaccggc
     3421 gtccgggcct gcccggccgg gcgcccggca ccaggtccgc accacgctcg aggaccagcg
     3481 cgtccaggac gcacgtcgcc ctcgccagca tgtgcaggcg ctcgtacaaa ggcacggacg
     3541 ggtcctccgc ctcctgcatg agtcgcgcgg agaacgccag gcgggccaga tcgcgtcgga
     3601 tcagggtctt cgtatcggac atggtcctat gccatacacc ccgggggcgc ggaagccccc
     3661 agcggaaacc gtggtcctcc cggggaatct acgcgagatt cggctcgcag accgcgttcg
     3721 ggaagacccg gaggattgcc cggccaggcg cccgccgccc tgcgcgaccg gtgcgggcgc
     3781 cgtagtttcc gtgccgccaa ccccacccgc ccatgcgcct ccttcccgtc cttttcgtcg
     3841 ccgccgtgct cctctcgtcg tgccgggcgc ggtccgagta cgaaatcacc ttcagcaccg
     3901 accgcgacga gtccctggac atattcggga tgacgcccga ctcgtcgtac ttccgcggat
     3961 atacgggcga cgacggcacc gagtacggcc cgcgctggtc cccggacggg agcgcgatcc
     4021 tgtacacggc gtggaggggc ggaaacgccg acatcgtggc gttcgagccc gagcgggggc
     4081 ggatccgccg cctggtgcag gcacccggct acgacgggaa tcccgactgg tcgtccgacg
     4141 gcgcccacat cgtcttcgtt tccgaccggg acggcgagcg gaagctgtac cggacgaggg
     4201 tcgacacgct ggacgtggtc cggctgtcgg gtccggaagg ggtggacacg tcgccccgct
     4261 ggtcgccccg gggcaacctc atcgtcttca cccggtcgga gcccgggacg accgaccgcg
     4321 gcgccgtcat ggtcatgaac gccgacggca ccgggcagcg caagctcacc tcgggcgatt
     4381 cgttcgacag cctggccgac tggtcgcccg acggatctcg catcgcattc caccgctgcg
     4441 acgacggctc ctgctccctc tggacgatgt cggtcgacgg aacgaacgag aacccgctgc
     4501 tcgatgacgg gcacgacaac cggtggcccg cctggtcgcc cgacggcgag tggatcgcgt
     4561 tcacatcgac gcgtgacggg cagtcggaca tctacgtggt ccgccccgac ggctccgacg
     4621 tccgccgcgt cacgcgctgg ccgggacggg acgaagggcc cgcctggcga cccgttccac
     4681 gcctgcccga ggcggcgagg caatagcggc ggggccgtag cggcgaagcg atcgcggaga
     4741 ggccgtagag gcgaggcgcc cgagggccgg ggccgtcagg cgatcctgag gtacggctcg
     4801 acgggcagcc ggtccgggta gcccgcgacg aacgcatcgc gcgagcggtc ggaatcgaag
     4861 aggtacgccg cgtacccgcc gtagcccccg ccggcgtact tgcaggcgag catgcccggc
     4921 gcctcgtcca ggggctgcat gccctcgccc acctgcatgg cgtacgagat gcgcacggcc
     4981 tcggccaggc cgcccggatc gcgttcccac gctgcgtccc tggcacgctt cccggcgcgc
     5041 tcgatggcgt cgaaatcccg cggacggtcg accactccgg gcgtgtcgtg cggccggccc
     5101 gtccaggcga gcgccatcct gcccgccagc atgctcccgt ccgtcttcag ctcgagcgac
     5161 ggccggctgc cgcttcgcca cacgcacagg cccgtctccc ggatcaccgc cggatcctgc
     5221 cagcccaccc cgaggtccag ctcggcctcc acgccctccc ggccgttgag cagtgcccac
     5281 gcgccgctgc ccccgaggcc ggagcggtgc ctgtacggcc agtccgtgag gctgacgagt
     5341 ggagatatgg cgcagttcac gatgaaggcc ccgtcgcgcg cgaacctggg cacgtccagc
     5401 catccgccgc cgaagtcgat gcgcagcggc gcctcgcgcg gcgcgcgtat ccaccggatg
     5461 agctcgctcg tcgagaccgg gtcgaaccgc ggcggcgtct tgggaagcac cacgtagcgc
     5521 gcgcccgtcc gcgcgcacag cgcccgcttc tcggcctcga acccgtcatc ctccgtgacc
     5581 gccaggatat cggggcgcat ccgctcgaac gcctcgatga agtcgagacc ggggtccgcg
     5641 cccgtaccga ccaccacgtc gtccaccatc tcgagcgcga cgaggagagc cctcttgtgc
     5701 tcgtccggca gcgagccgcg ccgccccttg tagcgcacga ggacgtcgga cgacgcgaag
     5761 ctcaccgtca ggcggtcgcc cagcgcccgc gcctcccgga agaactgcag gtgaccggcg
     5821 tgcaggatgt cgtaacagcc cgagacgaag acgtgggtca tgagcggggt ccgccgcgag
     5881 ggagtccgaa tatactcccg gcgtccggcg gatccgttcg ccgccggtcc gtgtaggaag
     5941 cccgctaccc cgacctgccc gcccggaacc atgcgcgctg ccctctttgc cgtggtcgcc
     6001 gcgttcgccg cgaccgcccc cgccgacgcc cagcccgtct ccatcgacga gtgggaagta
     6061 ccgtacgcag agtcccgacc ccgcgacccc tacgtggccc ccgacgggcg ggtctggttc
     6121 tgcggccagc gcacgggcta cctcgcagtg ctcgatcccg cgaccggcga gtaccggaaa
     6181 tacgacctcg gcgagggagc aggcccccac aacctgatcg tggacgacag cggcatggtc
     6241 tggtacgcgg gcaaccgccg cgcgcacatc ggccggctcg atcccgcgac cgggaacgtc
     6301 acgaagttcc ccatgccgga cgacgccgcc cgggatccgc acacgctcgc ctgggacggg
     6361 gagggcgaca tctggttcac cgtgcagggc ggcaacttcg tcggtcacct ggacaccggg
     6421 tccggcgagg tgcgcctcgt ggccgtgccc acagagcggg cccgcccgta cgggatccgc
     6481 gtcgatcccg acggccgtcc gtggatagcc ctcttcggca cccacaagct ggcgaccgtc
     6541 gatccggcca cgatggagct caccgaggtt ccgctgtcca gggaatcggc ccggccgcgc
     6601 cgggtcgaga tttcgtccga cggccgagtg tggtacgtgg actacgcgga cggcatgctg
     6661 ggcgcgtacg atcctcgaga cggatccatc gaggagtggg cgatcccggg cggcccggat
     6721 tccaggccgt acggtacggc cctcgacgag cgcgaccgga tctggttcgt ggagacgggc
     6781 ccgcagccca accggctcgc gggattcgac acgagggccg ccacgtggct cgacgcgacg
     6841 ccgatcgaaa gcggggcggg atccgtccgg cacatgtatt ttcacccgcc cacacgcacc
     6901 atctggttcg ggacggatgc gaacacgatc ggccgcgcgc ggctcgcgga tgacccgggc
     6961 cggtagcctc tccctgctgc tccccgtgct cgtgctgggc cttcctcatc cggacggtcc
     7021 gccgcccgcg cacacgggcg gcttcggcga gcccacctgc acggactgcc acgcggattt
     7081 cgaggtcaac gagggcccgg gcggtctcgt cctgaccggc ttcccggagc ggatatcccc
     7141 gggagcggtg tacgacctga aggtattcct gcgtcacgag gggatggccc gtgcgggatt
     7201 ccagctgtcg gcgagaaccc cggacggcgc gcaggctgga agactcgcgc ccgtctcgga
     7261 tcggaccgcc acggcaacgg aggacggcat agagtatgcc ggacacaccg aggccggaag
     7321 cttcgccgcc ggcgattccg cggcctggac cgtccgctgg accgcgcccg attcgatcgc
     7381 cggcgacctc gtcgtccacg tgacggccaa cgcggccaac ggcgacgact ccccgttcga
     7441 agactgggtc tacgccggcg cgtggaccct gggcatccac tgattcacga ttacggcccc
     7501 tgcaccatcc atgcggcacc tggcatcgct ctccctcgtg gttctcctca cggcgaccgg
     7561 cgcaagggcg caggtcgtgg actcgacgcg cacggacgac gatctcgagc gggagctcga
     7621 gcgggagctc gcggcccctg ccccgccgcg gcaacggtcc ggcacgaatc ccgacatcag
     7681 cgtcatcggc gacacgcgcg cgtggaccgt ctccgagggg gagcggaacg tggacctcgg
     7741 ggtccacgag gtggagaccg ccttccggtc cacggtcgac ccgtacgcgc gggctgacgt
     7801 ctacctttcg gtcggctcgg aggacgggga attcgagttc gagctcgagg aggcctacct
     7861 gaccacgctg tcgctgccgg cgggcctcca gctcaaggcc ggcaagtttc ggagcgcgtt
     7921 cgggaagatc aaccgcaccc atccacacgc cctgccgttc atcgacgtgc ccgcgccgta
     7981 cgccaacttc ctgggggacg aggggctgaa cgaccagggc atctccgtga actggctcgt
     8041 cccgaatgcg cgtttctatc aggaggtgtc gttcgaggtc acgcgcgggc ccgccgaaag
     8101 cgccagcttc gaaacggacg acggcaaccg tctcctgtac gcggggcgct tcatgaactt
     8161 ctgggacctg tcgcccgacg ccaccctcga ggtcggagtg tcggccgcgt cgggaccgaa
     8221 cccgtcgggc cggacgagcg aactcgtggg cctggacgtg acctaccgct ggaagccgct
     8281 gcggtacaac acgtaccgga gcttcctcct gcaggcggag ggactcctca gccgcaaccg
     8341 gaccgacctg ggcacggtcg acggcttcgg attctacgcc ctggccgggt accagctcgg
     8401 ccgccggtgg tcggtgttcg gccgcttcga ccacgccgac ctgcccgacg acgcggactg
     8461 gaacgagtac gggatttccg ggaccctcgg ctggtacctg accgagttcc agaagctgga
     8521 attcggcctg cgccacgcct gggccgatgg catggatccg gtctggcagg gtctcgtgag
     8581 gctggttttc gttatcggaa cccacggtgc gcacgagtat tagccccacc ggtccgtccg
     8641 tgcccgagcc gcctcgggca agcccccgca ccggagtcga catgcgacgc ctcagcccgt
     8701 acatccaggg actcgcgcgg cacctcgccg cgctcctgtt tgccgtctgc ctcggcacga
     8761 cggcctccgc gcagccgaag ctgcaggtcg tcgcgacgct gaccgacctc aagagcatcg
     8821 cggaggagat cggcggcgac cgggtcgagg cgtttgcgat cgccaccggc ttccagaatc
     8881 cgcatttcgt cgatcccaag ccgagctaca tcgtccggct cgcgcgcgcc gacctgttcg
     8941 tgaccgtcgg cctcgacctc gagaccgggt gggtaccccc gctgctgaac agcgcgcgca
     9001 acccgcgcat cctccccggg gcgtccgggt acgtggacgc gtccgcgaac gtgccccttc
     9061 tccaggtgcc ctcgagcgtg agccgcgagc agggcgacat ccacgtctac ggcaatccgc
     9121 actactggct ggaccccgag cgcgggaaga tcgtggcccg caacatcgcg gacgggctga
     9181 aacggctcga gcccgggagc gccgactatt tcgaggagaa cctcgagcgc ttcacggccg
     9241 ccatcgacca gaagatgctg gagtggacgg tccggctcgc gccgtacgcc ggcacgcccg
     9301 tcatcgccta ccacaacgag tatccctacc tcgagtcggc gttcggcttc cgcatcgttg
     9361 atttcctcga gcccaagccc ggcatcccgc cctcgcccgc gcagctggcc cgggtgatcc
     9421 ggaccatgaa ggaccggggc atccgcgtga tcatcatctc gccgtacttc aacccggacg
     9481 cggcggagct ggtcgccggc aagatcgccg gcggcaccgt ggtcacgatc gcgtcgtcgg
     9541 tcgaggcctt ccacggcatc gagacgtact tcgacctctt cgattacaac gtggaccagc
     9601 tggtccgggc cttccagcag actcccggcc cgtagcgctc cccatgagcg atctcttcgc
     9661 cctcgatttc gtgcggaacg cgctgctcgt cagcgtcatc atgggcctga tgctctccta
     9721 cctcggcgtc cacgtcgtcg ggcgcggcat cgtcttcgtg gacctggccc tgggacagat
     9781 ttcgatgctc ggcgtcgcgg tcgccaccta cttcgagacg gacgccacga cggtgtcggt
     9841 ggtgttcact ctcgtgggcg cgatcctgct ctcgttcatc aagaccacgg accgccgcct
     9901 gaagcaggag gcgatcatcg gcatcatcta cgcggtgtcg tccgccgtga cggtgctcct
     9961 gatttcgaag gccgcccacg gcgacgccga catccaggag gtgctcttcg gcagcgtctt
    10021 caccgtcacc ccggcgcaga tcgggtggat ggcgggcgtg ttcggcgtgc tcgccctggt
    10081 ccacgtcttc ttccgccggc agctgttcga ggtcacgcgg aagtacgagg cgaaggaggt
    10141 ccaggacctg ggcgccttca acctgtggaa cttcgtgttc tacgtgtcga tcggactcgc
    10201 catcgtgctc gccgtgcgcg tgggaggcgt gatcccggtg ttcgcctacc tcgtggtccc
    10261 gcccgtggcg ggcatcctga tgacgacccg aagccatgcc ggcgtcgtgc tgatcgcgct
    10321 cccgatcagc gtggtgggca gcctggcggg cgtatacttc tcggtgcagt tcgacttccc
    10381 ggccggaccg tcggtggtcg cgatgctggg cgcgatcttc gcggtcgccg cgatcgtccg
    10441 gctcttccgc ggccgggtgg cccccgggcc cgccgaggcc tagcgccgga ccgtctcgat
    10501 gggcatgggc ccgtccggga ttccctcggc cgccttcgcc tcccggacca tcgccctcac
    10561 gtcctcgagc aggcgcggcg cgtcgaagac cacgccgccc ctgatcgtcc agcggacgcc
    10621 gcccacccgc tccacctccc cggtggcgtc gttcaggcgg agcgtgccgt tgccgtagag
    10681 cactttcagg ttctcgagcg gattctccgg cacgatgacc aggtccgcct gcttgccgac
    10741 gtggatggat ccgatgtctc gatcggcccc gagcgcctcc gcgccctgga tcgtggccga
    10801 tcggatcacc tcgagcggat ggaagccggc ctcgcgcagg agctccatct cctggatgta
    10861 cccgaatccg tacaggttgt agatgtagcc cgagtccgag cccagggtca cgcggccgcc
    10921 ccggttcttg tactcgttga cgaaggccat ccagagccgg tagttctcct tccagtccga
    10981 ctcgttctcg ctggtccagt agaaccagta cgacccgtgg gcggcccggc tcggccggta
    11041 gtagcgccac agcgagggca gcgtgtactc ggcgtggaac gaccggcggc tctgcgcctc
    11101 caggtcacgg ctcgccaggt acgacacgaa cgtcgggtcc agcgtgaagt cgagcgcgag
    11161 cagcgagtcc atcacggcgt tccagtgccg ggagaagggc gcggccgcct gtttccagag
    11221 ccgtccggcc tccccgaagc ggtggctctc gtcctggtag ttgtagtcca gtcggtactg
    11281 ctggatcacg cggtcctcga agagcgcctc cggaaggccg taccagtgct ccatcgacgt
    11341 gaggccggcg cgcgccgtcc ggagcacgtt catccgggtg acatcgagct gcgcatgatg
    11401 ctccgttccg cgcagccccc gtgccctcat ctcgtccagc gcggcacgca tcacttcggg
    11461 tggcgccccg aagaacttga tgccgtccac cccgcggccg gcgacccacc gcacccattc
    11521 ccgggcggca tccggcgtgt ctgttcccgc gtcggcgccc gctccgaaca ccgggtacag
    11581 gaaataacgt ggcgccgcta tccggttctc cgcgctcagc cgcctcgcct cgagcatggc
    11641 atccacgtcg ccgccggcca gctcccggac cgtcgtgatg ccgtgcgcca gccacagctt
    11701 tcggacgtac tcggccgaga cgccctggcc ggaaccctcc ccgtgggtgt gcccgtgcat
    11761 gtcgatgaac ccgggcagca catactgacc gtgcgcgtcg atctcccggg tgccgggggc
    11821 cggacgccgg gtcgagtcga tcggaagtcc cgggtatccg accgtccgga tctcggcgat
    11881 ccggtcgccc tcgatcacga tgtcgacggg gccgacgggc ggcgctcccg cgccgtcgat
    11941 catgattccg ccgcggatca ccaggcggtc gaacgggccc tctccggcgg gcgcccccgg
    12001 gtcctggcgg gccgcgacgg gccgcgttgc ggtcacgaga agaacgagca ggagcaggga
    12061 gaacgcgcgc atgggtccgg attgcgatgg tccgggatgc tacgggcttc cggccggaat
    12121 gccaacgccg gggcccggcg ccgtaaattg tccccatgca gacacgtacg aagaaggccc
    12181 cggacgggag cgttccgggt ccggcagtct cccgcggaac actcgtcctc gccctcgcgc
    12241 tgctcctcgc ccccgcccgc ccgtccggcg cgcagccagt agtcatcagc gagctccagg
    12301 cggacaacgc caccacggtg gccgatccgg acttcggggc ctttgcggac tggatcgagc
    12361 tgcacaacgc aggcacggcc gccgtcgacg tgggcgggta cttcctgtcc gacgatcccg
    12421 atctcccggg ccgctggcgg attccggacg gtacggcgat cgcgccgggc gcctacctgg
    12481 tcgtctgggc ggacgggctg gacacgggcc tgcacgcctc gttcgccctg ggccgggacg
    12541 gggagacggt gagactgtcc gacgcggcgg gcgtcccact ggacgaggtc gtgttcgggc
    12601 ccatgccctc cgacgtgtcc ttcggccgcc ggcccgcgga tccctcgacg tgggggtatc
    12661 tcgccacgcc gacgcccggc gcggccaacg ccggcccagc ctacgatggc ttcctggacc
    12721 cgcccgcatt cgatccgccc ggcggattct tcgccgttcc cgtgcaggtg acggcagcct
    12781 ccggggatcc ggcagcggtc gtgcgcttca cgacggacgg taccgcgcct cacgatgcct
    12841 ccccggccgc ggacggcccg atcgaagtgg ccgcgaacac ggtcctccgg gccgccgcgt
    12901 tccgccccgg ccatccggcg agcccggtca cgacggcgac gttcttcgtc ggcgagtccc
    12961 ccacgctccc ggtcgtttcg ctcgcgacgg atccggacaa tttcttcgac gacgagatcg
    13021 ggatctacgt cgcggggacg aacg
//
LOCUS       DBNHQZ010000172        10153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_865289, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000172 DBNHQZ010000000
VERSION     DBNHQZ010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_865289"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="AC16YU_04525"
     CDS             complement(<1..267)
                     /locus_tag="AC16YU_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064522.1"
                     /translation="MRRLLSNRIVLALAFLVAVVAVRYGSSAMLGGDGAPAPEPTTAV
                     RDAVSGPGAPSSANVSHEFRRRVAELRDRITAAPADTAAIRELAG"
     gene            complement(281..1153)
                     /locus_tag="AC16YU_04530"
     CDS             complement(281..1153)
                     /locus_tag="AC16YU_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064523.1"
                     /translation="MSRSPARMLALSATGVLWLALAGAALVAGLSYYRMPLVERPYSD
                     LHDVIGSSGQVGHAYGIVGTLMMLVGVATYSTRKRWRRLEHAGTLRTWLSFHIFLCTL
                     GPFLVLLHTTFRFGGLISIAFWSMTAVVASGIFGRYVYVRLPRGGAGGVVPPVVLRRR
                     RVELEERIGRGAPASSEWSSRLFGHGPADAEPGLAGALFRALRFDLDRRRLPGRLRSE
                     LEGLGVPGEDVGVLVADLVSYRETRHEMITYRPFQRLFGYWHVFHLPLAIVMLLIALL
                     HVGVAIAFGYVWIF"
     gene            complement(1327..2346)
                     /locus_tag="AC16YU_04535"
     CDS             complement(1327..2346)
                     /locus_tag="AC16YU_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6064524.1"
                     /translation="MDSLTQIALGAAVGEAALGHRVGRVAAGWGAVLGTVPDLDVLLY
                     PFLDAVDRLGMHRGISHSIAFALVAAPLFGMALARLHRARGEPAGRWGCLAFQVIITH
                     IALDCLTVYGTQIFQPFSNHPVGLDSIFIIDPLYTLPLAAGVLWTLVRRDAPRRGRAN
                     LAGLALSTAYLALTLVNKAAVDHSFRAGLEAEGIRPERVMTAPTPLNNMLWMGVAEAR
                     DTLWVALTGLLDDGPPNRFTAVPRRTGLVDGHEGDRAVRSLEWFSKGWWAAEVDSSGA
                     LYVNDLRFGRTDSWLADSGEYVFRFRLLESGGRYANHEQVPPDLRMSGEVLRGIADRI
                     RVRGG"
     gene            complement(2473..4821)
                     /locus_tag="AC16YU_04540"
     CDS             complement(2473..4821)
                     /locus_tag="AC16YU_04540"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6064525.1"
                     /translation="MAARPEPRPAVPSRLICRLAAALAVAGCAVPAYAQAQVRGTVTD
                     ARAGYPLPGASVQVAGTTRGTVTGLDGRFELEVRHFPSRLEVRHVGYRSAAVELGRVP
                     DAPLDIGLDPEIIGLEELVVTDESVAEGIMHRAIERRQAWRDSAKTAYADAYSRYILM
                     REFEPVRVEETLSAVWWKKGHGTREVVRARRSRPRRDERLRFAGPLPVPDLSDETIEL
                     YGTRFVGPLHPESPSVYRFTLFGRHLEDGRAVFDVAFVPARTDAAAFTGYLSVEDSTF
                     DVLSVTASPAVDRVGPPPILERVVRMEQSFARVDGGTLPAGFRAEGHVRFGMPGATYP
                     TARFRQVVGLAGHAIRVPAPDSLFESERLLRDTPDVRFSDWLFDGSHALVAPSAEDTI
                     ALERVIGAPPLARAFRPRGLLRSLLALDLVEATGPAGFGVERRVRWKRPWAWFNRVDG
                     WQVGLQPFIPVGEAWSVMPRLGYAEARGGVPWGIELAREVPDGFLAGFGADEYSAVVG
                     DDVRYSRFLLGLAAYCGWTDDHDYYARRRAYVRAGWTSGPGRASLTLSVERHRSEPRH
                     DFHEGLLVRNEQRANLPIQEGRLVGLTLNLSTGDALLPGYHWAAYAGSVILVARRGML
                     GSDFGFVRWSGAASIRFPTFDVRRQRPASLRLDLYFGLASDGTPVQLEGTLQHPVGPL
                     APGAGFWVSDARLPLVRHWIAAFWEHDFGSLVGELAGFPESSVALGVFGGHAVSADDE
                     NTGHHEVGLFVSNVLGYPVRLNVGSSVERFRLGLTVSVSQSF"
     gene            complement(4821..6419)
                     /locus_tag="AC16YU_04545"
     CDS             complement(4821..6419)
                     /locus_tag="AC16YU_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HIR6064526.1"
                     /translation="MNFSTIDYIVFGGYVALIVFLGLWVSREKKGHEKNTEDYFLASK
                     SLPWWAIGASLIASNISAEQFIGMSGSGFRVGLAIATYEWMAAVTLLIVAYFFLPIYL
                     KKGIFTMPQFLEVRYDTRVRTLLAVFWLLVYVFVNLTSVLYLGALAMEGIMGIDLWYG
                     VIGLALFAGLYSVYGGLKAVAWTDVVQVIFLVGGGLLTTYMALDAFGGEQGALAGFSR
                     LLTEAGDRFNMILFKGELMYEEAGELKDAYELLPGLSVLLGGMWIANLFYWGCNQYII
                     QRALAAKSLKEAQRGVAFAGYLKLLLPLVVVIPGIVAYALNAPIERGDQAYPWLLNAY
                     VGPGLKGLAFAALIAAIVSSLASMMNSTATIFTMDIYRNYVRKGASEREMVKIGRTVA
                     VVCLVIAVLLAPQLAGLDQVFQYIQEYTGMVSPGVLAIFMMGLFWKKSTPNAALVSAI
                     LSIPLSILMKYALPGLPFIDRMGVAFLASVALIVVISWIENKGKDHAKAIEIESDWRQ
                     TDPVFAVLAFGILGITAALYIIFW"
     gene            6806..9043
                     /locus_tag="AC16YU_04550"
     CDS             6806..9043
                     /locus_tag="AC16YU_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6064527.1"
                     /translation="MTSFLSRRQFIRLGAVGAGTLAVAPTLFRRRPTAESTFPLQAGD
                     TVTVTPTYCEICFWQCAGWVHAKNGRPWKIIGNEEDQHSYGRMCTRGTGGIGAHTDPD
                     RLRTPLIREEGPRGQRFREATWNEAFDYIAEKLASVREKYGPESLAMFSHGNGATHFK
                     TLTRAFGSQSIAAPSYAQCRGPRDEAFNLTFGEEVGSPERTDIENARCLVLVGSHIGE
                     NLHNGQVQEMSRAIERDARIIVVDPRFSVAASKADHWLAIKPATDLALLLAWIHVIIE
                     EGLYDREYVERYTTGFDELKEAVRTSTPEWAWPITTIEPAVIRETARTMARNAPATLV
                     HPGRHATWYGDDTQRMRAVAILNALLGSWGRKGGFYYPRSASVPAPAIPPFPTPRWTW
                     RDLAPKDFPFASSAVASLIRDAAADPAMAPDHPVKAWLVYGSNLVYALPNPERTIEAI
                     QNLDLLVAVDIQPSEICGWADVVLPECTYLERYDDLRILPGRTPQIALRAPAFEPLYD
                     TKPAAWMVKQLAARMGLDDWFPWVDMEDYLEQRLQRIGSSLEEMRRIGVMTLPGGSPL
                     YIQPGEDVRFNTPSGKIELHSDQLAAAGFDGVPRFTPHPEPPPGYYRLIQGRAPAHTF
                     ARTTNNPLLNELMSENDLWVNTATAEDWGLENGRYVHLRNQDDRLSQFPIRVRVTERI
                     RPDCVYMVHGFGHTEKLLTKAFGRGADDNELLADVRVDPIMGGTSRRNTFVTFEMSSA
                     PVEAV"
     gene            9044..9592
                     /locus_tag="AC16YU_04555"
     CDS             9044..9592
                     /locus_tag="AC16YU_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6064528.1"
                     /translation="MAGKHRYAMVIDTKLCIGCADCVVACKTENEVPEGYCRDWIAEA
                     TSGTFPELSLEYRSERCNHCDFPPCVHACPTGASFVDEESNVTLVDPGLCTGCKSCIA
                     ACPYDARFVMPEGYVSKCTFCIHRVREGLDPACVSACPTHCMHFGDLDDPTSEVSRLL
                     RTRTWKVIAPGVGTEPNVYYLT"
     gene            9603..>10153
                     /gene="nrfD"
                     /locus_tag="AC16YU_04560"
     CDS             9603..>10153
                     /gene="nrfD"
                     /locus_tag="AC16YU_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6064529.1"
                     /translation="MEELITTQKLNPHVDPALHIWGWEVPVYLFLGGLAAGLLLLGAA
                     SVLRREDGRPAAQRLLLAVTPLLGIGMGALFLDLEHKLHVWRFYTAFVVTAPMSWGSW
                     ILLAAVPVNLLLVAATAGRVWPEGRARAEGRVPGVGRLVAWAEPRTRALAWTSVVLAV
                     AVGIYTGILLSAYGARPFWNTAIL"
BASE COUNT         1701 a         3619 c         3492 g         1341 t
ORIGIN      
        1 cccggcgagc tcgcggatcg ccgcggtgtc ggcaggcgcc gcggtgatcc ggtccctgag
       61 ctccgcgacc cgccggcgaa actcgtgact gacgttggcg gacgaggggg caccgggtcc
      121 ggaaacggcg tcccggaccg cggtggtagg ctccggagcg ggggcaccat cgccacccag
      181 catcgcggac gatccgtacc gcaccgccac cacggcgacg aggaaggcga gggcgagcac
      241 gatccggttg ctgaggagtc ggcgcattgt cggggcgaag tcagaagatc cacacgtacc
      301 cgaacgcgat ggccacgccc acgtgcagga gcgcaatcag cagcatcacg atggccagcg
      361 gaagatggaa gacgtgccag tagccgaaca ggcgctggaa cggccggtag gtgatcatct
      421 cgtggcgggt ctcccggtac gaaacgagat ctgcgaccag cacgccgacg tcttcccccg
      481 gaacgcccag gccttcgagc tccgaccgga ggcggcccgg aagccggcgg cggtccaggt
      541 cgaagcggag cgcccggaac agggcgcctg ccagacccgg ctcggcgtcc gccgggccgt
      601 gcccgaacag gcggctggac cactcagagc tcgcgggcgc gccgcggccg atccgttcct
      661 cgagctcgac gcggcgccgg cgcaggacga ccggcggaac cacgcctccc gcgcccccgc
      721 gcggaagccg gacgtagacg tagcgcccga aaatcccgga agccacgacc gcggtcatgc
      781 tccagaacgc gatcgatatg agccccccga accggaacgt cgtgtgcagc agcaccagga
      841 acggcccgag cgtgcacagg aagatgtgga agctgagcca ggtgcgcagc gtgcctgcgt
      901 gctcgagcct gcgccatcgc tttcgcgtgc tgtacgtggc gacgcccacg agcatcatca
      961 gggtgccgac gatgccgtac gcgtggccca cctgtcccga cgagccgatg acgtcgtgca
     1021 ggtcggagta cggccgctcg acgagcggca tacggtagta cgacagcccg gcgaccaggg
     1081 ccgcaccggc cagggcgagc cagaggactc cggtcgccga gagcgcgagc atccgggcag
     1141 gtgaacggga caaggatggc gtatgatcgt gcccgggttc cgggaatccg gccggacgtg
     1201 gcaccgcagg tatcggccgg gacgtcgccc cgtgttaggc gcggccagcc cggatgatcg
     1261 cgcgcgccgg cagcacgcac aagcgcagac ggattcctgc ccctccaaag gaggcggccc
     1321 cgggcgtcat ccgccccgca cccggatgcg gtcggcaatc ccgcggagca cctcgccgct
     1381 catccggagg tccgggggca cctgctcgtg attcgcgtag cggcccccgg actcgaggag
     1441 ccggaaccgg aagacgtact cgcccgagtc cgcgagccag gagtcggtgc gcccgaagcg
     1501 aaggtcgttc acgtacagcg cgccggagga atccacctcc gccgcccacc agccctttga
     1561 gaaccactcg aggctccgga cggcccggtc gccctcgtgg ccgtcgacga ggccggtgcg
     1621 gcgcgggacg gccgtgaacc ggttgggcgg cccgtcgtcg agcagcccgg tcagcgcgac
     1681 ccagagcgtg tcgcgcgcct cggccacgcc catccacagc atgttgttga ggggcgtagg
     1741 ggccgtcatc acgcgttccg ggcggatgcc ctccgcctcg aggcccgccc ggaacgaatg
     1801 gtccacggcc gccttgttga cgagcgttag ggccaggtag gccgtcgaca gcgcgaggcc
     1861 ggcgaggttc gcccgccccc gtcgcggagc gtcgcggcgc acgagggtcc acagcacgcc
     1921 ggccgcgagc ggaagcgtgt acaggggatc gatgatgaag atcgagtcca ggcccaccgg
     1981 atggttcgag aacggctgga agatctgcgt gccgtagacc gtcaggcagt cgagcgcgat
     2041 gtgcgtgatg atgacctgga atgcgaggca cccccaccgc cctgccggct ctccgcgcgc
     2101 gcggtgcagg cgggcgagcg ccatgccgaa gagcggggcc gcgaccagcg cgaacgcgat
     2161 cgagtgcgag atcccccggt gcatgcccag ccggtccacg gcatccagga acgggtagag
     2221 cagcacgtcc aggtcgggca cggtgcccag gaccgcgccc cacccggcgg ccacgcgccc
     2281 cacgcgatgg ccgagcgcgg cctcgccgac ggcggcgccg agcgcgatct gggtgaggga
     2341 atccacgggg gcgggagtcg gggtgagcgg gacgggcggt cggagcggcc caggccgtcg
     2401 gggcggtcgg ggcaccgtcc aacgtggacg ggtcgcggcg gttcgcctgc ccggtcgtcg
     2461 cccgtccggc gctcaaaagg actgggacac cgacaccgtc aggcccaggc gaaaccgctc
     2521 cacggacgac ccgacgttca ggcgaaccgg gtagcccagg acgttcgaga cgaacaggcc
     2581 gacctcgtgg tggccggtgt tctcgtcatc cgcggaaact gcgtggccgc cgaagacgcc
     2641 gagggccacg gaactctccg ggaatccggc caactctccg accagggatc cgaagtcgtg
     2701 ctcccagaag gccgcgatcc agtggcggac gagcggcagg cgcgcgtcgg agacccagaa
     2761 ccccgccccc ggcgccagtg gcccgaccgg gtgctgcagg gtgccctcca gctgcacggg
     2821 cgtcccgtcg ctcgcgagcc cgaaatacag gtccaggcga agcgatgccg gccgctgccg
     2881 ccgcacgtcg aacgtgggaa agcggatcga cgcggccccg ctccaccgca cgaatccgaa
     2941 gtccgagccc agcattcccc gacgggccac caggatgacc gacccggcat aggccgccca
     3001 atggtacccg ggcaggaggg cgtccccggt cgacaggttc agcgtcaggc cgacgagccg
     3061 cccctcctgg atcggcagat tcgcccgctg ctcgttgcgc accagcagcc cctcgtggaa
     3121 atcgtggcgc ggctcggacc ggtgtctctc caccgacagc gtcagggacg cccgccccgg
     3181 gccgctcgtc caccccgcac gcacatacgc ccggcgacgc gcgtagtaat cgtgatcgtc
     3241 cgtccagcca cagtaggccg ccaggccgag caggaatctc gaataccgga cgtcgtcacc
     3301 cacaacggcc gagtactcgt ccgcgccgaa gcctgccagg aatccgtccg gaacttcgcg
     3361 cgcgagctcg atcccccacg ggacgccccc ccgagcctcc gcgtaaccga gccgcggcat
     3421 cacgctccac gcttccccga cgggaatgaa cggctgcagc ccgacctgcc agccgtccac
     3481 ccggttgaac caggcccagg gcctcttcca ccggactcgc cgctcgacgc cgaagcctgc
     3541 ggggccggtc gcctcgacga ggtccagcgc gagcaagctc cggagcagtc cccgcgggcg
     3601 gaatgcgcgg gcgagcggcg gcgcgccgat cacccgctcg agggcgatcg tgtcctcggc
     3661 ggaaggcgcg accagggcat ggctcccgtc gaagagccag tccgagaacc tgacgtccgg
     3721 cgtgtcgcgg aggagtcgct ccgattcgaa gagggaatcc ggcgccggga cgcggatggc
     3781 atgcccggcg agtccgacca cctggcgaaa cctggccgtg ggatacgtgg ccccgggcat
     3841 gccgaagcgg acgtgcccct cggcccggaa gcccgcgggc agagtaccgc cgtcaacgcg
     3901 ggcgaacgac tgctccattc tcaccacgcg ctcgaggatg ggcggcggac cgacccggtc
     3961 gacggccggg ctcgcggtca ccgacagcac atcgaaggtg gaatcctcga cggacaggta
     4021 gcccgtgaag gccgcggcgt ccgtcctcgc cgggacgaac gcgacgtcga acaccgcgcg
     4081 gccgtcctcc aggtgcctcc cgaagagcgt gaaccggtac accgacgggc tctccgggtg
     4141 cagcggaccg acgaaccggg tcccgtacag ctcgatcgtc tcgtcggaga gatccggcac
     4201 cggaaggggc ccggcgaagc gaagccgctc gtcgcgtcgt ggccgactgc gccgcgcacg
     4261 gaccacctcg cgggtgccgt gccccttctt ccaccagacg gccgaaaggg tctcctccac
     4321 ccggaccggc tcgaattccc tcatcaggat gtaacgggag tacgcgtcgg cgtaggccgt
     4381 tttcgccgag tcccgccacg cctgcctccg ctcgatggcg cggtgcatga tgccctccgc
     4441 cacgctctcg tcggtcacca caagttcctc gaggccgatg atctccggat cgagcccgat
     4501 gtccagcggg gcatcgggca cccggccgag ttccaccgcg gccgaccggt acccgacgtg
     4561 ccgcacctcg agcctcgaag ggaagtgccg gacctccagc tcgaaccgcc cgtcgaggcc
     4621 tgtgaccgtc ccgcgcgtcg tgccggcgac ctgcaccgaa gcgcccggca ggggataccc
     4681 ggcgcgggcg tccgtgaccg ttccgcggac ctgggcctgg gcatacgccg gaacggcgca
     4741 ccccgcgacc gcgagagcgg ccgccagccg gcagatgagg cgcgacggga ccgcaggccg
     4801 tggctccggt cgggccgcca tcaccagaat atgatgtaca gcgcggccgt tatgcccagg
     4861 atgccgaagg ccaggacggc gaagaccggg tcggtctgcc gccagtccga ctcgatctcg
     4921 atggccttcg cgtggtcctt ccccttgttc tcgatccacg agatcacgac gatgagcgcg
     4981 accgacgcca ggaacgccac gcccatccgg tcgatgaagg gcagtcccgg cagcgcgtac
     5041 ttcatcagga tggagagcgg gatcgacagg atcgcggaga cgagggccgc gttcggcgtc
     5101 gacttcttcc agaagagccc catcatgaag atggccagca cgcccggact caccatgccc
     5161 gtgtactcct ggatgtactg gaagacctgg tccaggcccg cgagctgcgg cgcgagcagg
     5221 accgcgatca ccaggcacac cacggcgacc gtccggccga tcttcaccat ctcccgctcg
     5281 ctcgccccct tccggacgta gttccggtag atgtccatcg tgaagatggt cgccgtcgag
     5341 ttcatcatgg acgcgagcga cgacacgatg gccgcgatga gcgccgcgaa ggcgaggccc
     5401 ttgaggccgg gccccacgta ggcgttcagg agccacgggt acgcctggtc gccccgctcg
     5461 atgggagcgt tcagcgcgta ggccacgatg cccgggatga ccaccacgag cgggaggagg
     5521 agcttcaggt atccggcgaa ggccacgccc cgctgggctt ccttgaggga cttcgcggcg
     5581 agcgcgcgct ggatgatgta ctggttgcag ccccagtaga agagatttgc gatccacatg
     5641 ccgccgagca gcaccgagag gccgggcagg agctcgtacg cgtccttgag ctcgccggcc
     5701 tcctcgtaca tgagctcgcc cttgaacagg atcatgttga agcggtcgcc cgcttccgtc
     5761 aacagccggg agaagccggc aagcgccccc tgctctccgc cgaaagcgtc gagcgccatg
     5821 tacgtggtca ggagcccgcc gcccaccagg aagatgacct gcacgacgtc cgtccatgcg
     5881 accgccttga gccctccgta caccgagtag aggcccgcga agagcgcgag gccgatcact
     5941 ccgtaccaga gatcgatccc catgatgccc tccatcgcga gtgcgcccag gtacaggacc
     6001 gacgtcaggt tcacgaagac gtacaccagc agccagaaga ccgcgagcag cgtccggacg
     6061 cgggtgtcgt agcgcacctc caggaactgg ggcatcgtga agatgccctt cttcaggtag
     6121 atcgggagga agaagtacgc gacgatgagc agcgtcacgg ccgccatcca ctcgtacgtg
     6181 gcgatcgcga gccccacccg gaagccggac cccgacatcc cgatgaactg ctcggccgag
     6241 atgttcgagg cgatgagcga cgcgccgatc gcccaccacg gcagcgactt cgaggccagg
     6301 aagtagtcct cggtattctt ctcgtgcccc ttcttttccc tggagaccca caggcccagg
     6361 aagacgatca gggccacgta gccgccgaac acgatgtagt cgatcgtgga gaaattcatg
     6421 gacgtgctgc ggatggaaga gcgacgcacc gcccgtcgtc gtccggggat gccccgggcc
     6481 gggcgggatg gatgcaccga ttctcgcctg cggggagcgc aatgtcaaca tggcgcccca
     6541 cgatgcggga ttccccggcg gggagcgtgg tgccggactt tgagaaggcg gtgaacatgc
     6601 cgtgcggcgc cccccggaag cctcgccgcc acgcgccggc ccggccgggc acgctcaagg
     6661 cggacggttc ggcgtagggt ttccccctat gcgacgccgg gggtgtgcgg cgggcatcca
     6721 tgggaaggtg gcggacgccc ggcgcgcccg gggggccgta ccatgggcac gcctgaaagg
     6781 gaacccccga cacggcctgc catccatgac ctcgttcctg tccagacgcc agttcatccg
     6841 cctcggggcc gtgggcgccg gcaccctcgc ggtggcgccc accctgttcc gccgccgccc
     6901 gacggccgag tcgacctttc ccctccaggc cggagacacc gtcaccgtga cgcccaccta
     6961 ctgcgagatc tgcttctggc agtgcgccgg ctgggttcac gcgaagaacg gacggccgtg
     7021 gaagatcatc ggcaacgagg aggatcagca ttcctatggc cggatgtgca cgcgcgggac
     7081 gggcggcatc ggcgcccata ccgatccgga tcgcctcagg acgccgctca tccgcgagga
     7141 ggggccccgg ggccagcggt tccgggaggc cacgtggaac gaggcgttcg attacatcgc
     7201 ggagaagctg gcctcggtcc gcgagaagta cggacccgag tcgctggcca tgttcagcca
     7261 cggcaacggc gcgacccact tcaagacgct cacccgggcc ttcgggtcgc agagcatcgc
     7321 cgccccgtcg tacgcccagt gccggggccc gcgggacgag gcgttcaacc tgactttcgg
     7381 ggaggaggtc ggctcgcccg agcggaccga catcgagaat gcccgctgcc tggtcctcgt
     7441 gggctcgcac atcggcgaga acctgcacaa cgggcaggtg caggagatgt ccagggcgat
     7501 cgagcgcgat gcccgcatca tcgtggtgga cccgcggttc tcggtcgcgg ccagcaaggc
     7561 cgaccactgg ctggccatca agccggccac cgacctcgcc ctgctcctgg cctggatcca
     7621 cgtgatcatc gaggaggggc tctacgaccg ggagtacgta gagcggtaca ccacgggctt
     7681 cgacgagctg aaggaggctg tccggaccag cacgcccgag tgggcctggc ccatcacgac
     7741 catcgagccg gccgtcatcc gggagaccgc ccgcaccatg gcgcgcaacg cgccggcgac
     7801 gctcgtccac ccgggccgcc acgcgacctg gtacggggac gacacgcagc gcatgcgggc
     7861 cgtcgccatc ctgaacgccc tgctcgggag ctggggcagg aaggggggct tctactatcc
     7921 ccgctcggcc tccgtgccgg cacccgcgat cccacccttc cccacgccgc gctggacgtg
     7981 gcgtgacctc gcgccgaaag acttcccgtt cgcctcgtcg gccgtcgcct cgctcatccg
     8041 ggacgccgcg gcggacccgg ccatggcccc cgaccacccg gtcaaggcct ggctcgtgta
     8101 cgggtccaac ctggtctacg ctctgcccaa ccccgagcgg acgatcgagg cgatccagaa
     8161 cctggacctc ctggtggcgg tggacatcca gccctccgag atctgcgggt gggccgacgt
     8221 ggtgctgccg gaatgcacct acctggagcg ctacgacgac ctgcgcattc tcccgggacg
     8281 cacgccgcag atcgccctcc gcgcaccggc cttcgagccg ctgtacgaca cgaagcccgc
     8341 ggcctggatg gtgaagcagc tcgcggcgag aatgggcctg gacgactggt tcccgtgggt
     8401 cgacatggag gactacctcg agcagcgcct ccagcgaatc ggctcgtccc tcgaggagat
     8461 gcggcgcatc ggggtcatga cgctgccggg aggatcgccg ctgtacatcc agccgggcga
     8521 ggacgtccgc ttcaacacgc cgagcgggaa gatcgagctc cattccgacc agctcgccgc
     8581 cgccggcttc gacggcgtgc cccggttcac gccccacccc gagccgccgc ccggatacta
     8641 ccggctgatc cagggccgcg cccccgccca caccttcgcg cggaccacca acaatccgct
     8701 cctgaacgag ctcatgagcg agaacgacct ctgggtgaac acggccacgg ccgaggactg
     8761 gggcctggag aacggccggt acgtccacct caggaaccag gacgaccggc tctcgcagtt
     8821 cccgatccgc gtgcgcgtga ccgagcgcat ccggcccgac tgcgtgtaca tggtgcacgg
     8881 cttcggccac accgagaagc tcctcacgaa ggcgttcggg cggggagcgg acgacaacga
     8941 gctgctcgcg gacgtccggg tggatcccat catgggcggc acgtcgcgga gaaacacgtt
     9001 cgtcacgttc gagatgtcga gcgcgccagt ggaggcggtc tagatggcag ggaagcaccg
     9061 gtacgcgatg gtcatcgaca cgaagctgtg catcggctgc gcggactgcg tcgtggcctg
     9121 caagaccgag aacgaggtcc cggagggcta ctgccgcgac tggatcgccg aggcgacgtc
     9181 cggcaccttc cccgagctct cgctcgagta ccgctcggag cgctgcaacc actgcgactt
     9241 cccgccgtgc gtgcacgcat gcccgaccgg ggccagcttc gtggacgagg agagcaacgt
     9301 gacgctggtc gaccccgggc tgtgcaccgg gtgcaagtcg tgcatcgccg cgtgccccta
     9361 cgacgcgcgc ttcgtgatgc cggagggcta cgtgtccaag tgcaccttct gcatccaccg
     9421 ggtgcgcgag ggcctggacc cggcctgcgt ctccgcctgc cccacgcact gcatgcactt
     9481 cggcgacctg gacgacccga cgagcgaggt cagccggctg ctccggaccc ggacctggaa
     9541 ggtgatcgcg ccgggggtcg gcaccgaacc caacgtctac tacctgacct gacgcgcgcg
     9601 agatggaaga gctcatcacg acgcagaagc tgaatccgca cgtcgatccc gccctgcaca
     9661 tctggggatg ggaggtgccc gtgtacctgt tcctgggcgg actcgcggcc ggactcctgc
     9721 tgctcggcgc cgcgagcgtc ctccggcggg aggacggccg gccggccgcc cagcgcctgc
     9781 tcctcgccgt gacgccgctg ctcggcatcg gcatgggggc cctgttcctg gacctggagc
     9841 acaagctcca cgtctggcgc ttctacacgg ccttcgtcgt cacggcgccc atgtcgtggg
     9901 gctcctggat cctgctcgcg gccgttcccg tgaacctgct gctcgtcgcg gcgacggccg
     9961 ggcgggtctg gccggaaggc agggcccggg cggaaggccg cgttcccggg gtcggacgcc
    10021 tcgtggcgtg ggcggagccg cgcacccggg cgctcgcgtg gacctcggtg gttctcgccg
    10081 tggccgtcgg catctacacg ggcatcctgc tgagcgcgta cggtgcgcgg ccgttctgga
    10141 acacggccat cct
//
LOCUS       DBNHQZ010000173         2286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_323599, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000173 DBNHQZ010000000
VERSION     DBNHQZ010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_323599"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="AC16YU_04565"
     CDS             <1..656
                     /locus_tag="AC16YU_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR6064530.1"
                     /translation="KPEYFVENQRVAANPHDQLKPRMSALIIASHVKEGIEIGLKHRL
                     PQRVLDFIPMHHGTTRIEYFYQRAVAQHKKGDAPIQESEFRYPGPRPASKETAILMLA
                     DTVEAACRTIDNPTHRRLEGMVETLVEARRADGQLDETDLTFHDLTIIRRTFLNLLLG
                     MYHVRVKYPGQAEEAGEAESPEGGDEAPAAEPAKAGKAGGPGGGDEALPSPADQRLE"
     gene            complement(679..1599)
                     /locus_tag="AC16YU_04570"
     CDS             complement(679..1599)
                     /locus_tag="AC16YU_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6064531.1"
                     /translation="MITLNRSDSRSLKDQLVGQVRFLVASGRFRIGERLPSTRTLASR
                     LSISFHTVRKAYAQLADEGVLEAVPGSGYRVLERHPETRGDRLEQGAPVMQAALRALV
                     GLGLSDSEMAYLFEEQLDLISSADESPRFVYVAEFREAAEEGAAFLEGLLGHGVEGIP
                     LARLESGSGADYAITALPLVRRVMEHLPRTDVLGVQVTLSPEATGAAARLYDHQTLLL
                     VVRYSDAVGPLSRMIKASSGFGGQIIALAVEEGDTRLGGLVRQSDLVLYTKGAHRALR
                     LHLASAASHRVVEVTVARSEVERLREELPG"
     gene            complement(1596..2117)
                     /locus_tag="AC16YU_04575"
     CDS             complement(1596..2117)
                     /locus_tag="AC16YU_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064532.1"
                     /translation="MENTFRSTRHPGGQPAAVLFGQALAGPVGICSLPVMIGALVTML
                     EGGRPLPFLTIGFPISLAVAWWWTASRLRTEIVELHVDGMLASFRSRWDVAVRRPHGA
                     GSAILDVRPSGVDLLLTIGLETIVLRRSEWPDLDLVRSACISAREEHVDTVRGRLDAP
                     ARQTPPIRRRPRP"
     gene            complement(2121..2270)
                     /locus_tag="AC16YU_04580"
     CDS             complement(2121..2270)
                     /locus_tag="AC16YU_04580"
                     /inference="COORDINATES: protein motif:HMM:NF040257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064533.1"
                     /translation="MDLVVHGGIFGDTATVAALVNTAPLVVGARPGLLTMKDIPLPRA
                     FAAVV"
BASE COUNT          392 a          789 c          823 g          282 t
ORIGIN      
        1 tgaagcccga gtacttcgtc gaaaaccagc gggtggcggc caatccccac gaccagctca
       61 agcccaggat gagcgcgctc atcatcgcga gccacgtcaa ggagggcatc gagatcggcc
      121 tcaagcacag gctcccgcag cgcgtcctcg acttcatccc catgcaccac ggcacgaccc
      181 ggatcgagta cttctaccag agggcggtgg cccagcacaa gaagggagat gcaccgatcc
      241 aggagagcga gttccgctat cccggcccga gaccggccag caaggagacg gccatcctga
      301 tgcttgctga cacggtcgag gcggcctgcc gcacgatcga caaccccacg caccgccggc
      361 tcgagggcat ggtcgaaacg ctcgtcgagg cccgccgcgc cgacgggcaa ctcgacgaga
      421 cggacctcac gttccatgac ctgacgatca tccgcaggac tttcctgaac ctgctcctgg
      481 gcatgtacca tgtccgggtg aagtacccgg gacaggcgga ggaggccggt gaggcggagt
      541 cgccggaggg gggtgacgag gcgccggccg ccgagccggc gaaagcgggt aaggcgggcg
      601 ggccgggggg cggcgacgag gccctgcctt cgcccgccga ccagagactg gagtagcgcc
      661 cggggcggtg cccggccgtc agccgggaag ctcctcgcgg agccgttcga cctcggaacg
      721 ggccacggtg acctcgacca cgcggtgcga ggcggcggac gcgagatgca accgcagtgc
      781 gcggtgcgcc cccttcgtgt acaggaccag gtcgctctgg cgtacgagcc cgcccagacg
      841 cgtgtcgccc tcctcgacgg cgagcgcgat gatctgtccg ccgaagccgg acgaggcctt
      901 gatcatgcgg ctcagcggtc cgacggcatc cgagtaccgg acgacgagca gcagggtctg
      961 gtgatcgtac aggcgcgccg ccgcgcctgt ggcctcgggc gagagcgtga cctggacgcc
     1021 gagcacgtcg gtccgcggca ggtgctccat cacccgccgg acgagcggga gcgccgtgat
     1081 cgcgtagtcc gcgccggagc ccgattcgag acgggcgagc ggaatgccct cgacgccgtg
     1141 gccgagaagg ccctcgagga aggcggcgcc ctcctcggcc gcctcgcgga actcggcgac
     1201 gtacacgaac cgggggctct cgtcggcgga agagatcagg tcgagctgct cctcgaacag
     1261 gtacgccatc tcggagtcgc tcaggcccag gccgaccagg gcgcggaggg cggcctgcat
     1321 gacgggggcc ccctgctcca gccggtcgcc gcgcgtctcc gggtgccgct cgagcacgcg
     1381 gtagccgctg cccgggacgg cctcgaggac accctcgtcg gccagctggg cgtaggcctt
     1441 ccgaaccgtg tggaaggaaa tcgaaagccg ggaggccagg gtcctcgtgg acggcaggcg
     1501 ttctccgatc cggaaccggc cggaggccac gagaaaccgc acctgcccga ccagctggtc
     1561 cttcagcgag cgggagtcgg atcggttcag cgtgatcacg gcctgggtct ccggcggatc
     1621 gggggcgtct ggcgggccgg cgcgtcgagc ctaccgcgca cggtgtccac gtgctcctcc
     1681 cgggccgaga tgcacgcgga ccggaccagg tccaggtccg gccactcgga ccggcgcagc
     1741 acgatcgttt cgagcccgat ggtaaggaga aggtcgacgc ccgagggtct gacgtcaagg
     1801 atcgccgaac ccgcaccgtg aggccgccgc acggccacgt cccaacggga acggaacgac
     1861 gccagcattc cgtcgacgtg gagctcgacg atctcggtgc ggagccttga cgccgtccac
     1921 caccacgcca ccgcgaggct gatcggaaag ccgatcgtga ggaaggggag aggccgcccg
     1981 ccctcgagca tggtcacgag ggcgccgatc atcaccggca gcgaacagat gcccaccgga
     2041 ccggcgagag cctgtccgaa gagcacggcg gccggctggc cgcccggatg ccgcgtgctt
     2101 cggaacgtgt tctccacgcg ctagaccacc gcggcgaagg cgcgcggcag cgggatgtcc
     2161 ttcatggtca ggagtcctgg ccgggcgccg acgaccagcg gtgccgtgtt gacgagcgcg
     2221 gccacggtgg ccgtgtcgcc gaagatgccc ccgtgcacca cgaggtcgat cggcgggtct
     2281 ccggcg
//
LOCUS       DBNHQZ010000174         4036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3711380, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000174 DBNHQZ010000000
VERSION     DBNHQZ010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3711380"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16YU_04585"
     CDS             complement(<1..376)
                     /locus_tag="AC16YU_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR6064534.1"
                     /translation="MKKYLTEFIGTFFLILTIGQVLAAGTLLAGLAIGSVLMVMVYMG
                     GHISGAHYNPAVTLGAWMRGKLQAGEAVGYMLVQLLGAIAAALVSWYLVDTTFAPAPA
                     GGVPQALVGEFIFTFALVLVVLN"
     gene            complement(469..1734)
                     /locus_tag="AC16YU_04590"
     CDS             complement(469..1734)
                     /locus_tag="AC16YU_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR6064535.1"
                     /translation="MAKERSRRRRNAARANQPDFRRPMLWAAVAASALGIALAAYSVQ
                     ISFTIAGQGIVEASGCSMGEFVNCDLAHASSYARLLGIPVAGWGLAFYVFAGLGALLG
                     LFSSNRRAAGGWIAFAWLLSIGAVVFSLVKAFHLYELGVLCIVCLGMYLVNVALVFLL
                     AAGLNAGGMGALLTNWLAAARGADSRLPFDAAAPKWALVAVVVFGLGFVVMKRQVDAV
                     ESGGDIDMELALAAHFRQTPLDVRVDPDAPLWGSENGIVQVVEFADFQCPACQVSAFH
                     LRTALFEFRDRLSFRFMNFPLDSAINEEMQGQVHAQAGAAAAASVCAQRFGDFWDYHD
                     DLFRNQVSLGPQLYTRLAQDRGWSVDEFEACMVGEDVRERIRSDIRAARDAGLSSTPT
                     IYLNGRRVAYWQNTEFIREVIREELRRAG"
     gene            complement(1756..2208)
                     /gene="arsN2"
                     /locus_tag="AC16YU_04595"
     CDS             complement(1756..2208)
                     /gene="arsN2"
                     /locus_tag="AC16YU_04595"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HIR6064536.1"
                     /translation="MRETRPAAPMVVRLDRDQLPRIRALLAQSGLSPEGLEADHVLLF
                     VTPDGEAPSGCAAVEVFGETGLLRSVAVAPARRGVGLGRALVAEAEEEARREGVRMLY
                     LLTETAEDFFAALGYRVVGREGLPGAVERSGQFKTMCPASAVVMTRDL"
     gene            complement(2201..2662)
                     /locus_tag="AC16YU_04600"
     CDS             complement(2201..2662)
                     /locus_tag="AC16YU_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphohydrolase domain-containing protein"
                     /protein_id="HIR6064537.1"
                     /translation="MPTPHFRRIRRFMQQAGQKTPDAVTVPDAPTRILRAKLILEEAL
                     ETVHALGVVVRAGGAEGPELVEGHLHYADGREPNLEMVVDGCADISVVTIGTLVAFGV
                     DDEPVLEEVDRANLRKFGEGSWRRDDGKWMKPPDWTPPDILGAIGRGAPDA"
     gene            2786..3451
                     /locus_tag="AC16YU_04605"
     CDS             2786..3451
                     /locus_tag="AC16YU_04605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6064538.1"
                     /translation="MLPFDPATIRAVLFDMDGVVLDSEPVAARVMQEAARHFGFDVPD
                     AAMDSYKGLPGEVVYARVAAEFGEGRVDGPRLRTLRDRLYETVLPGIPLMPSVRVVLD
                     IIAARDWTMALVTSSRRRHAESVVDTHGLRPYFPVILGSEDVSRPKPDPDPFVTAATS
                     LGQRPEACLAVEDSANGARSAAAAGCTVIGFGSGSPPTVLVEAGARVTVPDHVALAIL
                     LRD"
     gene            complement(3472..>4036)
                     /locus_tag="AC16YU_04610"
     CDS             complement(3472..>4036)
                     /locus_tag="AC16YU_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR6064539.1"
                     /translation="VLGGLKVNGAGHGFIEYRYPWPPDVEVESVSEARFVVEASAKQR
                     FDKDRPDPSAMDDDYMRGGGTLSPHRNPNAYPMTDQTDYPTSVTVEVNGLQAATAALA
                     DDPADHRGILSWHAQPRDRRLREAGSYGFLVQATVPPEALRRAAETGELVVRLAVGEE
                     SPGGLAVYGRSFGRYPLDPTVVLTLVE"
BASE COUNT          662 a         1450 c         1362 g          562 t
ORIGIN      
        1 tgttcaggac gacgagcacg agcgcaaacg tgaagatgaa ctcgccgacg agcgcctgcg
       61 ggacaccccc ggccggagcc ggcgcgaagg tggtatccac caggtaccag ctcacgagcg
      121 ccgcggcgat ggcaccgagc agctgaacca gcatgtagcc gacggcctcc ccagcctgga
      181 gcttcccgcg catccaggcc ccgagcgtga cggccggatt gtagtgtgca cccgagatgt
      241 gcccacccat atacaccatc accatcagca cgctgccgat cgcgaggccc gcaagcaggg
      301 ttccggcggc caggacctgg ccgatggtga gaatcaggaa aaacgtcccg atgaactcgg
      361 tgaggtactt cttcatgacg gtcgacggac ggtggacgtg aagggtccac ggacgatacc
      421 acttccgccc gtcgccgaca accgggagag cagccggggc cggcccgctc agcccgcccg
      481 gcgcagctcc tcccggatca cctcgcgaat gaattccgtg ttctgccagt acgcgacacg
      541 gcggccgttg aggtagatgg tcggggtcga gctcagcccc gcgtcccgcg cggcccggat
      601 gtcggaccgg atgcgctcgc ggacgtcctc gcccaccatg caggcctcga attcgtccac
      661 ggaccagccc cggtcctgcg cgagccgggt gtacagctgc ggaccgagag acacctggtt
      721 cctgaacagg tcgtcgtggt agtcccagaa atccccgaag cgctgcgcgc acacggaggc
      781 cgccgccgcc gctcccgcct gggcgtggac ctggccctgc atttcctcgt tgatggccga
      841 atcgagcggg aagttcatga agcggaacga gagccggtca cggaactcga acagggcggt
      901 gcgcaggtgg aatgcggaaa cctggcacgc cgggcactgg aaatccgcga actccacgac
      961 ctggacgatg ccgttttcgg agccccagag cggtgcgtcc ggatcgaccc gcacgtccag
     1021 gggcgtctgg cggaaatgcg ccgccagtgc gagctccatg tcgatgtcac cgccggactc
     1081 gacggcatcc acctgccgct tcatgaccac gaatccgagc ccgaagacca cgaccgccac
     1141 gagggcccat ttcggcgcgg cggcgtcgaa gggcaggcgc gagtccgcac cccgggcggc
     1201 ggccagccag ttcgtcagca gcgcccccat gcctccggcg ttcaggccgg cggcgagcag
     1261 gaagacgagc gcgacgttga ccaggtacat gcccaggcac acgatgcaga gcacgccgag
     1321 ctcgtacagg tggaaggcct tgacgaggga aaacaccacg gctccgatcg agagcagcca
     1381 ggcgaacgcg atccagccac cggccgcccg ccggttggac gagaacaggc cgagcagcgc
     1441 gccgaggccc gcgaagacgt agaatgcgag accccagccc gcgaccggga tcccgagaag
     1501 cctggcatag ctgctcgcat gggccaggtc gcagttgacg aactctccca tcgagcatcc
     1561 gctcgcctcc acgatcccct gcccggcgat cgtgaaagag atctggaccg agtacgcggc
     1621 aagcgcgatc cccagcgccg acgcggcgac cgcggcccac agcatgggcc tcctgaaatc
     1681 gggttgattc gcccgggccg cgttccggcg gcgacgggag cgttccttgg ccatgctctc
     1741 tggtcggtgt gggtgttaca ggtcccgggt catgacgacc gcggacgcgg ggcacatggt
     1801 cttgaactgc ccggagcgct ccacggctcc cggcaggccc tcgcgtccga ccacccggta
     1861 gcccagggcg gcgaagaaat cctcggccgt ctcggtcaga agatacagca tacggacgcc
     1921 ttcacgccgc gcctcctcct ccgcctccgc gacgagagcg cgtccgaggc caactccgcg
     1981 ccgggcgggc gccaccgcga ccgagcgcag gaggccggtc tcgccgaaca cctccacggc
     2041 cgcgcatccg gacggcgcct ccccatcggg ggtgacgaac aggagcacgt ggtcggcttc
     2101 caggccctcc gggctcagcc ccgactgggc caggagggcc cggatccggg gcagttgatc
     2161 ccggtcgagc cgcacgacca tcggcgccgc gggacgggtc tcacgcatcc ggcgcgccgc
     2221 ggccaatcgc ccccaggatg tcgggaggag tccagtccgg gggcttcatc cacttgccgt
     2281 cgtcccgccg ccagctgccc tcgccgaact tgcggagatt ggcccggtcc acttcctcga
     2341 ggaccggctc atcgtcgacc ccgaaggcaa ccagcgtccc gatcgtcacg accgagatgt
     2401 cggcgcagcc gtccaccacc atctccaggt tcggctcccg tccgtccgcg tagtgcaggt
     2461 gcccctcgac gagctccggc ccctccgcgc cgcctgcccg gaccacgacg cccagggcgt
     2521 gtacggtctc gagggcctcc tccaggatga gcttcgcccg gagaatgcgc gtcggcgcgt
     2581 cggggaccgt gacggcatcg ggtgtcttct ggcccgcctg ctgcatgaac cggcggatcc
     2641 ggcggaaatg aggagtcggc atgcggggag gttcgcggga tgatcggggg gctcggccac
     2701 cgcggaacaa ccggggcggt ccgacgaaag ataggcgcgt cgcgcgcgcc cggcgaccgg
     2761 agttttccac agcccgaaca cgtacatgct ccccttcgat cccgcgacga tccgggccgt
     2821 gctcttcgac atggacggcg tcgtcctcga ctcggaaccg gttgcggccc gggtcatgca
     2881 ggaggcggcg cgtcacttcg ggttcgacgt gccggacgcg gccatggact cgtacaaggg
     2941 cctccccggc gaagtggtgt acgcccgcgt ggccgccgaa ttcggagaag gccgcgtcga
     3001 cggaccccgg ctgcgcacgc tccgcgatcg gctctacgag accgtgctcc cgggcatccc
     3061 cctcatgccg tcggtccgcg tcgtcctcga catcatcgcg gcgcgggact ggaccatggc
     3121 gctcgtcacg tcgtcgcgac ggcgccatgc cgagtcggtc gtcgacacgc acgggctccg
     3181 gccgtacttc cccgtcatcc tgggatcgga agacgtctcg cgacccaagc ccgatcccga
     3241 tccgttcgtc accgcggcca cgagcctcgg ccaaaggccg gaagcctgcc tggcggtgga
     3301 ggactcggcc aacggcgcgc gctcggccgc cgcggcaggc tgcaccgtga tcggcttcgg
     3361 ctcgggctcg cccccgacgg tcctggtcga ggccggggcc agggttaccg tgcccgacca
     3421 cgtggccctg gccatccttc tgcgggactg acgggacacg ggtccgggcg gctattcgac
     3481 gagcgtgagc accacggtcg gatcgagcgg atagcggccg aacgaccgtc cgtacacggc
     3541 gaggccaccc ggggactcct cgcccaccgc gaggcgaacg acgagttcgc ccgtttccgc
     3601 cgcccgccgg agagcctcgg gcggcaccgt cgcctgcacc aggaatccgt aggatcccgc
     3661 ctcgcgcagg cgccgatccc gcggctgggc atgccacgac aggatgccac ggtggtcggc
     3721 cggatcgtcc gcgagcgcgg ccgtcgcggc ctggaggccg ttcacctcga ccgtgaccga
     3781 ggtcgggtaa tcggtctggt ccgtcatggg gtacgcgttt ggattgcgat gcggactgag
     3841 ggtgcctccg ccccgcatgt agtcgtcgtc catcgccgag ggatccggac gatccttgtc
     3901 gaagcgctgc ttggcggatg cctccacgac gaaccgtgcc tccgagacgc tctccacctc
     3961 gacgtcgggc ggccacggat atcggtattc gatgaagccg tggccggcgc cgttcacctt
     4021 caggccgccg agaacg
//
LOCUS       DBNHQZ010000175         2752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3711768, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000175 DBNHQZ010000000
VERSION     DBNHQZ010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3711768"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC16YU_04615"
     CDS             <1..392
                     /locus_tag="AC16YU_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064540.1"
                     /translation="GELYQDVGTVQYAPQSMLGQAAAPVMLDLANAGLAPDLRELPDE
                     FELYPNYPNPFNPVTVIGYDLPESGRVTLTVYDVLGRRVADLVNEEQTAGRYRILFDA
                     RQYASGVYLYRLNVGNFTKVGKMMLVK"
     gene            603..1937
                     /locus_tag="AC16YU_04620"
     CDS             603..1937
                     /locus_tag="AC16YU_04620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064541.1"
                     /translation="MGVEYQALEHDPSLVEWRRRAADVVILAGVGLHFVTAVIPVVLM
                     GAKYGPSFVPIFVGTWLATCFAAAARRIDHRVRVWLVLAALYVTSFSGALVMPDGPLL
                     RAWPVLAPILAIGLLGAREGRIATAISAVALLAVPILLSSPAAAQLWRVGLRPPGPTS
                     EFLLLQGPVLMAEMIIFMILLDRHHVFLLRSIASERRAVQDHAAAAARLENVIREQRQ
                     LEKDIAIARDEERRRLGNDLHDGVCQQLAGALLRCHALEIGLERGASASVQDVRALSS
                     LLGDTITETHAVAQGLWPLDSSPMALKGGLGRLVKQVRETHGARCEFTSDGNVEIADP
                     GTAQHLFRIAQEALGNAVRHARADLISVRLTGSDDLLTLSVQDDGAGFEQGGVTGGLG
                     LRTMTYRSHVIGGDLDISSEPGTGTRIECRVPRSESLRKAEEAEPYLADHPP"
     gene            1934..2593
                     /locus_tag="AC16YU_04625"
     CDS             1934..2593
                     /locus_tag="AC16YU_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6064542.1"
                     /translation="MRSPQSDSARPLSILVVDDHLAVRAGLSQLVAADGMVVSNQVAS
                     LRQTLECVDACRPDAAIVDLMLDGEDGLAVVSTLAARRIPVLVYSMHADGQHVRMALS
                     AGALGYVTKAESHEVLTDGLREISSGSRYLSPKAAVALAAEVSGHNGSDSMPILSRKE
                     REVFRRLGEGDGTAEIAEAMRISVHTVESYYARIQLKLGIQGMYDLRHRAIEAARHHL
                     T"
BASE COUNT          446 a          931 c          900 g          475 t
ORIGIN      
        1 cgggcgagct ttaccaggat gtgggcacgg tgcagtacgc gccgcagtcg atgctgggcc
       61 aggcggccgc tccggtgatg ctcgacctcg ccaacgcggg gctcgcaccg gacctgcgcg
      121 agctaccgga cgagttcgag ctgtatccga actacccgaa cccgttcaac ccggtgacgg
      181 tgatcgggta cgacctgccc gagtccggcc gggtgaccct gacggtgtac gacgtgctcg
      241 gccgccgcgt ggcggacctg gtgaacgagg agcagacggc cgggcgttac cgcatcctgt
      301 tcgacgcgcg gcagtacgca agcggcgtct acctgtaccg cctgaacgtg ggcaacttca
      361 ccaaggtcgg gaagatgatg ctggtcaagt agatccggac cgatcggaat cagcgagagg
      421 cgggggcccg gcgacgggtc cccgcttttc attttccggg gtctcgctgc tctgccgacc
      481 cgcccggcca cggctcgctg tgtagggtgc cggatgacgg ccgctcgctc gtttgaccga
      541 tagagcggag gcccttccgt tgttattaat ggccccgcct cccgtgcacc acgtacccga
      601 cgatgggtgt cgaataccag gcacttgaac acgatccgtc actcgtcgag tggcgccgcc
      661 gggcggccga cgtcgtgatc ctggcgggag tcgggctgca cttcgtgacc gcggtcatcc
      721 cggtggtgct gatgggagcg aagtacggac cttccttcgt cccgatcttc gtcggtacgt
      781 ggctggcgac gtgcttcgcc gcggccgctc gccggatcga ccaccgcgta cgagtctggc
      841 ttgtactcgc tgcactgtac gtgacgtcgt tctctggagc gctcgtcatg cctgacggtc
      901 ctctcctgcg cgcatggccg gtgcttgcac cgatcctggc catcggcctc cttggcgccc
      961 gtgagggccg tatcgccacc gccatcagcg cggtcgcctt gctggccgta ccgatcctgc
     1021 tttcctcgcc ggccgctgca caattgtggc gtgtcggcct cagaccgccg gggccgacca
     1081 gcgagttcct gctcctgcag ggccctgtac tgatggcgga gatgatcatc ttcatgatcc
     1141 tcctggaccg acatcacgta ttcctgctgc gctcgatcgc ctccgagcgt cgcgccgtcc
     1201 aggatcatgc ggccgctgcc gccaggctgg agaatgtcat tcgcgagcag cggcagctgg
     1261 aaaaggacat cgcgatcgca cgtgacgagg aaaggcgtcg actcggaaac gatctccacg
     1321 acggcgtgtg ccagcagctc gccggggcgc tcctccgctg ccacgcgttg gagatcggac
     1381 tcgagcgcgg agcgtctgcg tcggtccagg acgtcagggc actctcgtcc ctgctgggtg
     1441 acaccattac cgaaacgcat gcggtggccc aggggctctg gccgctcgat tcctccccga
     1501 tggccctcaa gggcggactg ggacgtctcg tgaagcaggt ccgcgagact cacggcgccc
     1561 ggtgtgaatt cacctcggac ggaaacgtcg agatcgcaga ccccgggacg gcccagcacc
     1621 tgttccgcat cgcacaggag gcactcggca acgccgtgcg ccatgcccgc gcggacctga
     1681 tcagcgtccg gttgaccggt tccgatgacc tgctgacgct gagcgtgcag gatgacggtg
     1741 ccgggttcga gcagggcgga gtcaccggcg gactgggact tcggaccatg acctaccggt
     1801 cacacgtgat cggtggcgat ctggacatca gctccgaacc cggcaccgga acccggatcg
     1861 agtgtcgcgt gccgcgctcg gagtcactcc gaaaagcgga agaagccgaa ccgtacctcg
     1921 ccgatcaccc cccatgagat cgccgcaatc cgattctgcc cgtcccctca gcattctcgt
     1981 cgtggacgat cacctcgccg tcagggccgg cctctcccag ctcgtcgcgg cggatggcat
     2041 ggtcgtttcg aaccaggtcg cctcgcttcg gcagacgctg gaatgcgtgg acgcatgccg
     2101 tcccgacgcg gccatagtgg acctgatgct ggacggggag gacggccttg cggtcgtttc
     2161 gacgctcgcg gcccggagaa tcccggtgct cgtctactcc atgcacgccg acgggcagca
     2221 cgtgcgcatg gccctctcgg ccggtgccct cggctacgtg acgaaggccg agtctcacga
     2281 ggtcctcacc gacggcctcc gggagatttc ctccggaagc cgatacctga gcccgaaggc
     2341 cgccgtggcc ctcgccgccg aagtgtcagg ccacaatgga tcggattcca tgcccatcct
     2401 cagccgcaag gaacgcgagg tgttccgtcg cctgggcgag ggggacggta ccgcggagat
     2461 cgccgaagcc atgcggatca gcgtgcacac ggtcgagtcg tactacgcca ggatccagct
     2521 caagctcggc atacagggta tgtacgatct gcgccaccgc gccatcgagg ccgcgcgaca
     2581 tcacctcacg tgaccgcgcc ctgccgtccc gtagtggttt tccacggcag ggcgtaggca
     2641 caatccgctg cagactcccg gggcggcctg aagtaggctc ggaatgacag gaccgagcga
     2701 gcttcacgcc catgaaggac cggcatcttc aggacacggc cgggataccg cc
//
LOCUS       DBNHQZ010000176         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_52823, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000176 DBNHQZ010000000
VERSION     DBNHQZ010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_52823"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="AC16YU_04630"
     CDS             complement(<1..882)
                     /locus_tag="AC16YU_04630"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR6064543.1"
                     /translation="MDPSLIKTPPRPDLLLRIADRVGTPTYVYFADTIRERIRVLRER
                     TERLDAHLLYAMKANAHPAILELMFDEGLGIDAVSPAELLLALRVGFAPERILFSANN
                     MTDDEMHFAHHQGVLLNVGELSRLDRYGSAHPGSRVCVRLNPRIGAGHHDHVITAGPE
                     SKFGIPVEAAGEIVRIIERHGLRLVGLHQHIGSGILDTGTLWSAIRVLLDAVRQFPPV
                     EFVNLGGGLGVPYRPGETGLDFENFDARIVEPLGEFLAGLDPRPAAWFEPGRFLTAES
                     GVLLVRVNTVKKNPERTF"
     gene            1082..1453
                     /locus_tag="AC16YU_04635"
     CDS             1082..1453
                     /locus_tag="AC16YU_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6064544.1"
                     /translation="MPEATLHRWEDLPLESLNERLDRRLVTGERIMLAHVYLKEGCVV
                     PMHRHENEQMTYILEGTLRFRLGEDGEREVVVRAGEVLHIPSNLPHEAVALEDTLDVD
                     IFSPPRQDWLQGTDHYLRAAR"
     gene            complement(1506..2474)
                     /locus_tag="AC16YU_04640"
     CDS             complement(1506..2474)
                     /locus_tag="AC16YU_04640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6064545.1"
                     /translation="MPGPRIFGAAKRTGPMGKIEDTYKAKDVEEIIDIHLYRPWGYAL
                     AVAALRLHMTPNQVSVIGMAVGVVSGHFFFYQSLPLNLVGIFLWMLAQALDGADGQLA
                     RMADMRSQLGRMLDGISDNVKFTSLYLHLGARLALEFDSPWPIAVMFASGIAHSYQSA
                     LADYYRNAYLWFVYGAGRAELEDEEELHAQYRALSWRRDPLMKFLSWGYLVYTRRQAR
                     WASGSLELIRDVSERYGEALPDALRAMYRALNRPLLRYYNALTTNTRMVALYVTVFIG
                     QVAWYFAFELVVLNLVMVLLLVRWQPEAHARVREFMEARPAGAPFS"
BASE COUNT          440 a          900 c          953 g          392 t
ORIGIN      
        1 gaacgtccgc tcggggttct tcttgacggt gttcacgcgc acgagcagga cgcccgattc
       61 ggccgtcagg aaacgcccgg gctcgaacca ggcggccggc cgaggatcca ggcccgcgag
      121 gaactcgccg agaggctcga cgatgcgggc gtcgaagttt tcgaaatcga ggcccgtctc
      181 gcccggccgg tagggcacgc ccaggccccc gcccaggttg acgaattcga ccggagggaa
      241 ctgcctcacg gcgtcgagga gcacccggat ggcgctccac agcgtcccgg tgtccaggat
      301 gccgctgccg atgtgctggt ggaggcccac caggcgcagg ccgtgccgct cgatgatccg
      361 gacgatctcg cctgccgcct cgacggggat tccgaacttg gactccggtc cggccgtgat
      421 cacgtggtcg tggtgtcccg ccccgatccg gggattgaga cggacgcaga cccggctccc
      481 cggatgggcg ctgccgtagc ggtccaggcg cgagagctcg cccacgttga gcagcacgcc
      541 ctggtgatgg gcgaagtgca tctcgtcgtc cgtcatgttg ttcgccgaga acaggatgcg
      601 ctccggggcg aacccgacgc gcagcgcgag caggagctcg gccggcgaca ccgcgtcgat
      661 tccgagcccc tcgtcgaaca tcagctccag gatcgccggg tgggcgttgg ccttcatcgc
      721 gtagagcagg tgcgcatcca gccgctccgt gcgctcgcga agcacgcgga tgcgctcccg
      781 gatggtgtcc gcgaagtaga cgtaggtcgg ggtcccgacc cggtccgcga tgcggagcag
      841 gagatcgggc cggggcgggg tcttgatgag gctcggatcc atgggggccg gttgagtggg
      901 gcgcgtcgcg gagcgggcgt gcggatgtcc gtgcggccgg cgcacccgca cgagcctacg
      961 gcggcacgga atcccgtgca agcggtgccg caggcgccgc cgccgccgat tgcgctgccg
     1021 cagggtcgat tccgtcgccg ggatgggtta ccatgcgacc gccaagcatt ccgacacccg
     1081 catgcccgaa gccacgctgc accgctggga agaccttccc ctcgaatcgc tgaacgaacg
     1141 gctggaccgc cgactggtga cgggcgagcg catcatgctc gcccacgtct acctgaagga
     1201 gggatgcgtc gtcccgatgc accgccacga gaacgagcag atgacctaca tcctcgaggg
     1261 cacgctccgc ttccggctcg gggaggacgg cgaaagggag gtggtcgtcc gggcgggcga
     1321 ggtgcttcac attccgtcga acctcccgca cgaggccgtg gcgctcgagg acacgctcga
     1381 cgtggacatc ttcagcccgc cgcgccagga ctggctgcag ggcaccgacc actacctgcg
     1441 cgccgcgcgg tgatccgcgc gtgaccccgg ggcgtgcgtc gccctggcgc cgcccgcacg
     1501 agcggtcagg agaagggagc cccggccggc cgggcctcca tgaactcgcg gacccgcgcg
     1561 tgggcctccg gctgccagcg cacgagcagc aggaccatga ccaggttcag taccacgagc
     1621 tcgaacgcga agtaccaggc cacctggccg atgaacaccg tgacgtacag cgccaccatg
     1681 cgggtgttgg tcgtcagcgc attgtagtac cggagcagcg gtcggttcag cgcccggtac
     1741 atcgcgcgga gcgcgtcggg aagcgcctct ccgtagcgct ccgatacgtc ccggatcagt
     1801 tcgagggacc cggacgccca ccgggcctgg cgccgggtat agaccaggta gccccacgag
     1861 aggaatttca tgagcgggtc ccgccgccag gaaagcgcgc ggtactgggc gtggagctcc
     1921 tcctcgtcct cgagctccgc tcgcccggct ccgtacacga accagaggta ggcgttgcga
     1981 tagtaatcgg cgagcgcgct ctggtacgag tgggcaatgc ccgaggcgaa catgaccgcg
     2041 atgggccacg gggagtcgaa ctcgagagcc aggcgcgctc cgaggtgcag gtacagggag
     2101 gtgaacttga cgttgtccga gatgccgtcg agcatccgcc cgagctggga ccgcatgtcg
     2161 gccatgcggg cgagctgacc gtccgcgccg tccagggcct gggcgagcat ccagaggaag
     2221 atccccacga ggttcagggg caggctctgg tagaagaaga agtggcccga taccaccccg
     2281 acggccatgc cgatgaccga gacctggttc ggggtcatgt gaaggcgcag cgcggccacc
     2341 gccagggcat agccccacgg gcggtacagg tggatgtcga tgatttcctc gacatccttc
     2401 gccttgtacg tgtcttcgat cttccccatc ggccccgtcc gtttcgcggc cccgaagata
     2461 cggggaccgg gcatggcgcg atacctccct ggccggccgg gcaccgggcg ggcgatcagg
     2521 tcgagacgcc gggcacccag tcggcgacga ccgggtcgag cagctcctcg atcgtggggc
     2581 ttccccgctg ctcgagatcg taccggacgc gcacggtcgg tttgtcgatc cgtacgacgt
     2641 gtcggaggtc gatcggcgtc gcgatgatca cgacgtccgc cggca
//
LOCUS       DBNHQZ010000177         3690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2896812, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000177 DBNHQZ010000000
VERSION     DBNHQZ010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2896812"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..242)
                     /locus_tag="AC16YU_04645"
     CDS             complement(99..242)
                     /locus_tag="AC16YU_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064546.1"
                     /translation="MDAILPLAYVVACHPAAAQTVGTCEVGSATIDLDGNSVRGRMRP
                     GGF"
     gene            828..>3690
                     /locus_tag="AC16YU_04650"
     CDS             828..>3690
                     /locus_tag="AC16YU_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR6064547.1"
                     /translation="MGQDLYDPRFEHDACGVGLVCRIDGTPEHRVVADGLAILSRLAH
                     RGATGCDPETGDGAGVLVQIPDAFFREEVPWELPAAGDYAVGVWFLPPGRNLRPAVVA
                     ACETAGLSVLGWRRVPVRSEAIGEAARTSEPDVWQCFVARPADADRGLAFERRLFVAR
                     RLGERAARKAAGPAAGGVHSPSFSSRVVVYKGMFTATQVGPYYPDLTDERLASAVALV
                     HSRFSTNTLPRWPLAHPFRYLCHNGEINTLRGNAGWMRARESLFETDAFGPHLAGVLP
                     ILEPGMSDSAALDAALELLLLSGRSLPKAMMMLIPEAWELHESMSPERRAFYEYQACL
                     MEPWDGPATVLFTDGRLAGAMLDRNGLRPSRYTVTHDGYIVLASESGTLDTPAGNVRE
                     EGRLQPGRMLLVDLEEGRLVSDDEVKGRIVSERPYGEWLGRHMVHVDDLPDTPHAPVD
                     PAAPAAPAEPLATRQRLFGWTREALEMLVAPMASGGKEAIGSMGRDVPVAVLSRQARP
                     LFDYFHQLFAQVTNPPLDAIRERLVTSLFTNLGGCRNLFEERPEHADVVRLDHPVLDG
                     AQLDRLKSARGAVTLDATHEPSGSGLREAVDRLEAEAEKAVDAGSRILVLSDRGAGRG
                     RAPVPSLLALGAVHQHLVRSGKRLSCSLAVESGEPREVHHFAALLGFGASAVHPYLLF
                     ESVRSLAGSSAAPDVTPDDAERNTTRAICDGILKVMSKMGISAVHSYRGAQIFEAVGI
                     GREVIERCFDGTTSRLGGIGFGEIATEYAAFHADAYPERAPADRPDLPSGGLYRWRRD
                     GEFHTLRPEVVARLQQAVRGDDAGAYEEYARLVNEPGRDRGTLRGLLRIRTGAATPVP
                     IDQVEPWTAIVRRFKTGAMSYGSISREAHETLAVAMNRIGGRSNTGEGGEEAWRYDRA
                     HPARSRIKQVASGRFGVTAAYFASADEIQIKMAQGAKPGE"
BASE COUNT          558 a         1298 c         1306 g          528 t
ORIGIN      
        1 atcagggcga tgaggactcc gaggttcccc gccatggagc ctgatctgct gcgatgacct
       61 cgtgtcattg aatttcccat ggcatccggt accggcggtc aaaaccctcc cggccgcatc
      121 cgcccccgga cgctgttgcc gtccaggtcg atggtcgccg atccgacctc gcacgtgccc
      181 acggtctggg ccgcggccgg gtggcacgcg accacgtacg cgagcggcag aatggcgtcc
      241 acacacctgg cgcggagaag cgcctgcccg ccaccggcgc gagccggtcg gcatcgtaac
      301 agtcgggaca tcatcaaatc gcggtcatcg tgagcgaatc ggctcggcga cgacagtccc
      361 gggtgggacg ccgactatag gagcgcctcc acggtggaat gagaaacgcg cgaagcggtc
      421 agcggtggtc tcgcctgccc ggaccgtcgt cagggtagtc cgacccgatc ccccgcggcc
      481 gatgtactgc ggaagggctg caagatcgtt tgcgtggcac tccatgttgc actgtttcca
      541 tagggggctt ctcgatacag gcgaacgagg cgccggcgcc cgcccgccgc cgccggccgc
      601 caccggccac cgcccgcccg ccgccaccgg ccaccgcccg ccgcccacaa agccaccccg
      661 aaagcgcttc cgaagttatt taggcccccg gacacacagc ctcaattgtg aaatcgtaag
      721 ccctcacggg gcaaaatgat tactggaaac gcttccataa tccatggata ttgcatggag
      781 ggcgggtcgt accgtatccc tctggtccac atagacttcc gcgttccatg ggccaggacc
      841 tgtacgaccc ccgcttcgag cacgacgcgt gcggtgtcgg gctggtctgc cggatcgacg
      901 ggacccctga gcaccgggtc gtggcggacg gcctcgccat cctgtctcgg ctcgcgcacc
      961 ggggcgcgac cgggtgcgat ccggagaccg gcgacggcgc cggcgtcctc gtccagatcc
     1021 cggatgcgtt cttccgggaa gaggtgccgt gggagctgcc ggccgccggc gactacgccg
     1081 tgggagtgtg gtttctgccg ccgggtcgaa acctgaggcc tgccgtcgtc gcggcctgcg
     1141 aaaccgcggg tctgtcggtt ctcgggtggc gccgcgttcc ggtccgaagc gaggccatcg
     1201 gcgaggcggc ccgcacgagc gagcccgacg tctggcagtg cttcgtcgcg agacccgcgg
     1261 acgccgatcg gggcctcgcc ttcgagcggc ggctcttcgt cgcgaggcgc ctcggcgagc
     1321 gggcggcgag gaaggcggca gggccggcgg ccggcggcgt ccattcgccc agcttctcct
     1381 cgcgtgtcgt ggtctacaag ggcatgttca cggccacgca ggtcgggccc tactacccgg
     1441 acctgacgga cgagaggctg gcgtcggcgg tggctctcgt ccactcgcgc ttctcgacca
     1501 acacgctgcc gcgctggccg ctcgcgcacc cgttccggta cctgtgccac aacggcgaaa
     1561 tcaacacgct ccgtggcaac gccggctgga tgcgcgcgcg cgagtcgctc ttcgagaccg
     1621 acgccttcgg gccgcacctg gcaggcgtgc tcccgatcct ggagccggga atgagcgatt
     1681 cggcagcgct ggacgcggcc ctcgagctcc tcctgctcag cggccgatcg ctccccaaag
     1741 cgatgatgat gctcatcccg gaggcctggg agctgcacga gtcgatgtcg cccgagcgtc
     1801 gggcgttcta cgagtaccag gcgtgcctga tggagccgtg ggacgggccg gccaccgtgc
     1861 tcttcaccga cggccggctg gcgggcgcga tgctggaccg caacggactg cgtccctcgc
     1921 gatacacggt gacgcacgac gggtacatcg tcctcgcctc tgaatcgggg acgctcgaca
     1981 cgccggcggg caacgtccgg gaggaaggcc gtctgcagcc gggccgcatg ctcttggtgg
     2041 acctcgagga gggccgcctg gtgagcgacg acgaggtcaa gggacggatc gtctccgaac
     2101 ggccgtacgg ggagtggctc gggcgtcaca tggtgcacgt cgacgacctc ccggacaccc
     2161 cgcacgcgcc cgtcgacccc gccgcacccg ccgcccccgc cgagcccctc gccacgcggc
     2221 agcggctctt cggctggacg cgtgaggcgc tcgaaatgct ggtcgcgccg atggcgtcgg
     2281 gcggcaagga agcgatcggt tcgatggggc gggacgtgcc ggtggcggtc ctgtcgcggc
     2341 aggcgcgccc gcttttcgac tatttccatc agctcttcgc ccaggtcacg aacccgcccc
     2401 tcgacgcgat ccgcgaacgc ctggtcacgt cgctgttcac gaatctcggc gggtgccgca
     2461 acctgttcga ggagcggccg gagcacgcgg acgtcgtccg cctggaccac ccggtgctcg
     2521 acggagcgca gctcgaccgg ctgaagagcg cgcgcggcgc ggtcacgctc gacgcgaccc
     2581 acgaaccctc gggatccgga ctccgagagg ccgtggatcg gctggaggcc gaggcggaga
     2641 aagccgtcga cgccggctcg agaatcctcg tcctctcgga ccgcggggcg ggacggggcc
     2701 gcgcgcccgt gccgtcgcta ctggccctcg gtgcggtcca ccagcacctg gtccgttcag
     2761 gcaagcggct ctcctgttcg ctcgccgtcg agagcgggga gccacgcgag gtgcatcact
     2821 tcgcggcgct cctgggcttc ggcgcgagcg cggtccaccc gtacctgctc ttcgagagcg
     2881 tacgcagcct cgccggaagc tccgcggccc cggacgtgac acccgacgac gccgagcgga
     2941 acaccacgcg cgcgatctgt gacggcatcc tgaaggtcat gtccaagatg ggcatctcgg
     3001 ccgtccactc gtaccggggt gcgcagatct tcgaggccgt ggggatcgga cgcgaggtca
     3061 tcgagcggtg cttcgacgga acgacgagcc gcctgggcgg catcggattc ggggaaatcg
     3121 cgaccgagta cgcggcgttc cacgctgacg cgtaccccga gcgggcgccg gcggaccgcc
     3181 cggacctgcc gtcgggcggc ctgtaccggt ggcgccggga cggggagttc cacacgctcc
     3241 ggcccgaggt ggtggcgcgc ctgcagcagg ccgtgcgcgg cgacgacgcc ggcgcctacg
     3301 aagagtacgc gaggctcgtc aacgagccgg ggcgagaccg gggcacgctc cgcggcctgc
     3361 tgcgcatccg gacgggcgcg gcaacgccgg tgccgatcga ccaggtcgag ccctggaccg
     3421 cgatcgtccg ccggttcaag accggggcca tgtcgtacgg gtccatcagc cgcgaggccc
     3481 acgagacgct ggccgtcgcc atgaaccgga tcggcggacg cagcaacacg ggggaggggg
     3541 gcgaggaggc ctggcggtac gaccgcgcgc acccggcgcg cagccgcatc aagcaggtgg
     3601 cctccggccg gttcggagtc acggccgcgt atttcgcgag cgccgacgag atccagatca
     3661 agatggcgca gggggcgaag cccggcgagg
//
LOCUS       DBNHQZ010000178        10460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1136584, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000178 DBNHQZ010000000
VERSION     DBNHQZ010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1136584"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC16YU_04655"
     CDS             complement(<1..857)
                     /locus_tag="AC16YU_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064548.1"
                     /translation="MLRNYLVIALRNLRKRPGYSFINIGGLALGLACTFLITLYVRQE
                     LSFDRFHRNGERTYRLLVENRGDRGTSRQAVSASGFAPDLLERYPEIEQAIRLDYRGG
                     SVRVDDRRFTPTDFLVADAEFFEVFTFPLVRGDPATVLDSPDALVVTESTARALFGDA
                     DPIGRMVNLSDRVDLRVTGVAKDPPPHTHLPFEYVASFGIMTTLMGPDALHEFSNYNY
                     YTYLLLRPGSDPRSLEAKLPGYLAATFDPETARSQGLLLQPLAEIHFTTDVAWDVPSN
                     TDARYVAIFS"
     gene            complement(1037..2056)
                     /locus_tag="AC16YU_04660"
     CDS             complement(1037..2056)
                     /locus_tag="AC16YU_04660"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6064549.1"
                     /translation="MRTMDVTRAIDDAIGQAMASDPTVVVLGEDVPLLRRALLVRFGP
                     ERVLGTPISESAFVGAGVAAAMAGLRPVVEVYMVDFLAVCLDALVNHAAKVDAFSGGR
                     WNAPVVVRAPCSAGYGDAGQHGQSLWSMMAHVPGLSVVVPSTPADAGGLMTTALAHTG
                     PVVFLEPKLLSESWLEFLGSGGRKTVDYDSPEEGIRGKVPRRWEPIPFGEAAVRRDGT
                     DVTVAGVGVAVHRALEAANRLEKDGISTRVIDLRTVSPLDVDAVARSVERTGRLLVVD
                     EDYEGFGLSGEIAAALLERGLVFRYGRVCTKNTIPYARHLEDHALPNVARIVEGAWAM
                     MERGA"
     gene            complement(2053..3078)
                     /locus_tag="AC16YU_04665"
     CDS             complement(2053..3078)
                     /locus_tag="AC16YU_04665"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6064550.1"
                     /translation="MAGPRDIASLYADMLKARLFEEAIASLWRDGMISGEMHMGTGEE
                     AVIAGVVSQLRDGDAMALDHRGSAPLLLRGVDPVLILRELLGRDDGLCRGMGGHMHLF
                     AREHLAASTGIVGAGGPTVAGFALAARMLRPGSVAVAFFGEGSLNQGMMMEALNLVSV
                     WKLPALFVCKDDDWSITTPSGGMTAGSVADRVRGFGMTPVEVDGLDVLAVQQASAEAV
                     DGVRGGSGPAFLHAKCIHLEAHFMGYPLLRAVRHPASELPALSAPLARSLLGRGASVR
                     ERVRGVHSVVSSLLATVRDARSDWENDPVVKARARLAPEDAQSIEHAVAEQIGEIVTI
                     ALGAAPS"
     gene            3213..4001
                     /locus_tag="AC16YU_04670"
     CDS             3213..4001
                     /locus_tag="AC16YU_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR6064551.1"
                     /translation="MRIPSLLLACLLLVTASASAQDAGLTDDQLLALYEGLRVADVAD
                     GMDVVGLRGVGLVDTRIQALWKDFDELDHQFHGIALTVRYVPHNLVVPNPIPADSFAA
                     WESTWYNEVSPEPFLDLIRPGTAIVIDAGGNGDTGSIGSFNSLEWTRRGMRGIVTTGS
                     ARDTDEIAKQQIPLYMDPLERGRGIRPGRNMIESVNQPVEIGGALVRPGDVVVADGDG
                     VQIVPREHAERVALIARGVLDKDMAGRRALYEQLGIPPDRTVGN"
     gene            complement(4014..5195)
                     /locus_tag="AC16YU_04675"
     CDS             complement(4014..5195)
                     /locus_tag="AC16YU_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064552.1"
                     /translation="MRCLSVILAILAVAARSAPAAAQFVDPFDSGDVSGWTWFTGDGE
                     AVMDFVAGDTTASIRVDASADYRNTWWALVKRNVTASLDLSRLASQGAELRIQARIRV
                     SHAPRRVNLHLNTQRTTDFHSHLVEFDIPDTLGWHTISMTTRGFDAAPGDTVYGQLAL
                     MDWGFGRYRVDLDEFRVEVVDPATAGPDHGTAVPYRPPVADPDSFAYRIMVAEDGMID
                     LGHAGVTVEDWYVVGEGRRERGLRADEDRMTILRWDLGEFTGRRVRGSGLLELTTLSV
                     ERAETDSAETGLLRVVEILGGDPEWTGATVTLQSFTAGRPLGAVLNPQMIIDVPVTGR
                     RGGRTLVTISEPVLQRLVDGRTRGLALRPLGAISAAFFTRDYFGGVAAAHLYFNLEPD
                     S"
     gene            complement(5200..6465)
                     /locus_tag="AC16YU_04680"
     CDS             complement(5200..6465)
                     /locus_tag="AC16YU_04680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6064553.1"
                     /translation="MTRATSAALAAALLLSACTPSEPAVDRVVDDAASARIDSALSAM
                     VEAGRVAGASALVFEKGREAYFGAFGHADREAGIPMARNTIVQIFSMTKPITGTALMS
                     LYEEGAFDLDDPVSRHVPEFADLGVYAGMDDSGRPMLEPPRREMTIRDLGRHTSGITS
                     DPGSPEAIELLTAAAPLDRENTLAEMARRFGTLPLWFHPGERWAYGPSVDVQAYLAER
                     LSGRPFGELVRERVLDPLGMTDTRYVVPGSDLGRFAALFSRGEDGVLVRVPDELGRSF
                     NMQAWPLTPGGFGLTSTLDDYMRFARMLLGGGELDGVRILQPETVRLMATNALADSVT
                     ERSWLPSKGQVGFGFDFAVRVRPPASASENNGVVGEFFWDGAATTLFWVDPVNELAAV
                     FFVQVFPFDGTLHKAIRDAVYGPVALPKP"
     gene            complement(6499..8973)
                     /locus_tag="AC16YU_04685"
     CDS             complement(6499..8973)
                     /locus_tag="AC16YU_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HIR6064554.1"
                     /translation="MTRSGPALLVLLLAAAPAALGQPTDLRLDDRGVLSARGLDVLAF
                     SNWYSGLFSDSKLSGVELIHHGVRTATNGDVRLSNTPEQWDPIPQLVDRIVDPESGAV
                     TARLAYPDHGFEYRIEVRPEAGGALVAVHLDAPLPAALEGRAGFNLEFLPSAYFGKTF
                     MMDDAGGAFPRIATGRRTMAPYGMLEAEPMATGGRLVLAPGDPERRVTIDGRGARLSL
                     YDGRTQAQNGWFVVRSLLPAGRTGRVLEWLVTPHRIPDWTRRPVVAHSQVGYHPRQEK
                     IAVLELDPHFEAPASARLLRILPDGRTEERFAGPALPWGPYLRYEYRRFDFTAVEEPG
                     LYVLEYAGVRSEPFRIDFDVYRSGVWQPTLDTFMPVQMDHVLVNDRYRVWHGAGHLDD
                     ALQAPVDHEHFDLYAQGPATDSPFEPGEHIPGLDVGGWHDAGDYDIRTQSQYATVQAL
                     VLARETFGIDRDQTTVRQEERFVDLGVPDGVPDIVQQVEHGVLALLAQYRAVGHAIPG
                     IIVPTIAQYTHLGDAITMTDNLVYDPSLDSLETDGHRSGTFDDRWAFTSRSTPLDYGS
                     IAALAAASRVLRGYRDALADECLSTAVRVWDEEQAREPVVFRWGNTTGGPLEIEEAKA
                     TVELLVTTGDRKYADRLGALWPGMEQRFGAYAALAVRALPFMDDGFRRSVEAAVRAYA
                     AGLDQVAASNPFGVPVTTGGWGGSGAVIGFAMTAHILHRAFPEIVSPEHTLRGLAYVL
                     GTHPASSVSMVSGVGARSMTTAYGANRADHSFIPGGIVPGIVIVQPDLPELKEDHPFL
                     WYEGEYVIPLGATFIYVAHAAESLFR"
     gene            9154..>10460
                     /locus_tag="AC16YU_04690"
     CDS             9154..>10460
                     /locus_tag="AC16YU_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6064555.1"
                     /translation="MRRLALLFLLAPLACSPADPEVAAWEERASGITIIRDDWGVPHV
                     YGETDADAVFGMIYAQAEDDFNRIEHNYLLSMGRLAEAEGEAEIWRDLRMKLFIDPDS
                     MRALYGRSPAWLRDLTDAWADGLNYYLHTHPEVTPRVITRFEPWMALTFSEGSIGGDI
                     ERVSLRDLESFYGQRGGSAIAGERQDAGPLVPADLYALAREPLVPLHAEPVGSNGFAI
                     APSNSATGNALLWINPHTSFFFREELHMVSREGLDAYGAVTWGQFFVYQGFNEGAGWM
                     HTSSGVDNIDEFLEAVVEREEGLVYRYGDGERPVVERVITVPYRSGDSLAHRTFMSYR
                     THHGPVVRAEGDRWVAVSLMEDPLGALAQSYSRTKARNYAEFRATMEGHTNSSNNTVF
                     ADSEGNIAYFHSNFIPRRDPRFDYARPVDGSDPATDWNGVLSI"
BASE COUNT         1664 a         3643 c         3627 g         1526 t
ORIGIN      
        1 gagaagatcg ccacgtagcg cgcgtccgtg ttcgacggca cgtcccacgc gacgtcggtc
       61 gtgaagtgga tctcggccag cggctgcagg agcaggccct gcgacctggc cgtctccggg
      121 tcgaaggtcg ccgcgaggta cccggggagc ttcgcttcga gcgagcgcgg gtcgcttccg
      181 ggcctgagca ggaggtaggt gtagtagttg tagttcgaga attcgtgcag cgcatccggc
      241 cccatgaggg tggtcatgat cccgaaggac gccacgtact cgaaggggag atgcgtatgg
      301 ggcggcggat ccttcgccac gcccgtcact cggaggtcga cgcggtcgga caggttgacc
      361 atccggccga tcgggtccgc atcgccgaag agcgcccggg ccgtcgactc ggtcacaacg
      421 agggcgtccg ggctgtcgag caccgtggcg gggtcgccgc gtacgagcgg gaacgtgaag
      481 acctcgaaga actccgcgtc ggcgaccaga aaatcggtcg gagtgaaccg gcggtcatcc
      541 actcggacac tgccgccccg gtagtccagc cgtatggcct gctcgatttc cgggtaccgc
      601 tcgagcaggt ccggcgcaaa accggaggcg gagacggcct gccgggaggt cccccggtcg
      661 ccccggttct cgacgagcag gcgatacgtc cgttcgccgt tccggtggaa ccgatcgaag
      721 gagagttcct gccgcacgta cagcgtgatc aggaacgtgc acgcgaggcc gagcgccagc
      781 cccccgatgt tgatgaacga gtatccgggg cgcttgcgca ggttgcgcag cgcgatgacg
      841 aggtagttgc ggagcatggt ccgggcggct gtactggggc tgtccagtag accggagaaa
      901 ttcacggaag gttgcaccgg gtcgggattg tcggcgatgc ggccgtacga accctctcac
      961 cggggcgaag gccgcgggcg acattgcgcg atgcgaagcc tgcgcgttcc tcacgaatcg
     1021 agcgcctcga gcccgatcac gctccccttt ccatcattgc ccaggcaccc tcgacgatcc
     1081 gggccacgtt cggaagagca tggtcctcca ggtgacgggc gtacgggatc gtgttcttcg
     1141 tgcacaccct cccatagcgg aacacgagtc ccctttccag gagggcggcg gcgatctcgc
     1201 cggacaggcc gaatccctcg tagtcctcgt ccacgaccaa cagccttccc gttcgctcca
     1261 ccgaccgggc tacggcgtcc acgtcgagcg gagaaacggt ccgaaggtcg atgacccgcg
     1321 tgctgatccc gtccttctcc aggcggttgg ccgcctcgag ggcccggtgc acggcgacac
     1381 ccacgcccgc caccgtcacg tccgtgccat cacggcgcac ggccgcctcc ccgaacggga
     1441 tcggctccca ccggcgtggc accttcccgc ggatgccctc ttcgggactg tcgtagtcta
     1501 ccgtctttcg acctccggat ccgaggaact cgagccacga ctccgaaagc agcttcggct
     1561 caaggaacac taccggtccg gtatgggcaa gcgccgtcgt catgagaccg cccgcgtcgg
     1621 ccggtgtcga gggcacgacg accgagagcc cgggcacgtg cgccatcatg ctccagagcg
     1681 actgtccgtg ctggccggcg tcgccatatc cagcggagca cggtgcacgc acgaccaccg
     1741 gggcattcca cctccctccg gagaacgcgt ccaccttggc agcgtggttg acgagggcgt
     1801 ccaggcagac cgccaggaaa tcgaccatgt acacctcgac gacgggacga agccccgcca
     1861 tcgcggcggc gacacccgcg cccacgaagg cactctcgct gatcggcgtc ccgaggacgc
     1921 gctccggacc gaagcgcacg agcagggcgc ggcggagcag gggcacgtcc tcgccgagca
     1981 cgaccacggt cggatcggaa gccatggcct gtccgatggc gtcgtcgatg gcccgcgtga
     2041 catccatcgt tctcatgacg gcgcggcccc cagcgctatc gtgacgatct cgccgatctg
     2101 ctccgcgacc gcgtgctcga tagactgcgc atcctccggt gccagccgcg cacgcgcttt
     2161 cacgacggga tcgttctccc agtcgctccg ggcatcccgg accgttgcca ggagcgacga
     2221 cacgaccgag tggacgcccc gcacgcgctc tcgcaccgac gcccccctcc cgagcaggct
     2281 ccttgcgagc ggcgccgaca gtgcgggcag ttcactggcg ggatgacgca ccgctctcag
     2341 gagcgggtac cccatgaagt gcgcctccag atggatgcac ttcgcgtgca ggaatgcggg
     2401 accactgcct ccgcgcacgc catcgaccgc ttcggctgaa gcctgctgga cggcaagcac
     2461 gtcgagcccg tcgacctcca ccggggtcat cccgaagccg cgcacgcggt cggcgacgga
     2521 gccggcggtc atgccgcccg acggggtggt gatggaccag tcgtcgtcct tgcacacgaa
     2581 cagcgcggga agcttccaca ccgacaccag gttcagggcc tccatcatca tgccctggtt
     2641 cagcgagccc tctccgaaaa aggccacggc caccgatccg gggcggagca tccgggcggc
     2701 gagcgcgaag ccggccacgg tcggtccgcc cgcccccacg attccggtgg atgcggccag
     2761 gtgctctctg gcgaacaggt gcatgtgccc tcccatgccg cggcacagcc catcgtcccg
     2821 accgaggagc tcgcgcagga tgagcaccgg gtccacgccg cgaaggagca gcggggcgga
     2881 cccccggtga tccagcgcca tggcgtcacc gtcgcgaagc tgtgacacca ctccggctat
     2941 gacggcctct tcccccgtgc ccatatgcat ttcgccggag atcatgccgt cccgccacag
     3001 cgaagcgatc gcttcctcga agaggcgcgc cttcagcatg tcggcgtaca gcgaagcgat
     3061 gtcccgtgga ccggccatcc gcaggttcgg gtgaggggcg cgaacccaat aaacggcttt
     3121 cgcctgtaag atgcgggtcc ttcctcgagc gggcgccgcg gagatcgtgc ggcggctatc
     3181 tttcccggca gcagatcagc cgagacgaaa cgatgcgcat cccatccctg ctccttgcct
     3241 gcctgctgct cgtaacggcc tccgcgtccg cccaggatgc cgggctgacg gacgaccagc
     3301 ttctcgccct ctacgaaggc ctgcgggtcg ccgacgtggc cgacggcatg gacgtcgtag
     3361 gactccgcgg cgtcggcctg gtggacacgc ggatccaggc gctgtggaag gacttcgacg
     3421 agctcgacca ccagttccac ggcatcgcgc tcaccgtgcg ctacgtgccg cacaacctgg
     3481 tcgtccccaa tcccattccc gcggactcgt tcgcggcctg ggagtcgact tggtacaacg
     3541 aggtttcccc cgagcccttc ctggacctga tcaggccggg cacggcgatc gtgatcgacg
     3601 ccggcggcaa cggggacacc ggatcgatcg ggtccttcaa ctcgctcgag tggacacgcc
     3661 gcggcatgcg cggcatcgtg acgaccggca gcgcccgcga caccgacgag atcgcgaagc
     3721 agcagattcc gctctacatg gatccgctcg agcggggccg gggaatacgg ccaggccgca
     3781 acatgatcga gtccgtgaac cagcccgtcg agatcggcgg ggcgctcgtg cgacccggcg
     3841 acgtggtcgt ggccgacggc gacggcgtgc agatcgttcc acgcgagcac gccgagcggg
     3901 tcgcgctcat cgcccgcggt gtcctggaca aggacatggc cgggcgcagg gcgctctacg
     3961 agcagctggg cataccgccg gacaggaccg tcgggaactg acgccccgcg ccgtcacgag
     4021 tccggctcca ggttgaaata caggtgcgcg gccgccaccc cgccgaagta gtcgcgggtg
     4081 aagaacgcgg cgctgatcgc gccgaggggc cggagggcca gcccacgcgt tcgcccgtcg
     4141 accaggcgct ggaggacggg ctccgagatc gtcacgagcg tcctgccccc gcgccgcccg
     4201 gtgaccggca cgtcgatgat catctgcggg ttcagcaccg cgccgagcgg ccggcctgcc
     4261 gtgaacgact ggagcgtcac ggtcgcgccc gtccactcgg gatctcctcc caggatttcg
     4321 accacgcgca gcaggccggt ctccgccgag tcggtttccg cgcgctcgac cgaaagggtc
     4381 gtcagctcga ggaggccgga tccacgcacc cgtcgacccg tgaattcgcc caggtcccat
     4441 cgcaggatcg tcatgcggtc ctcgtccgcc cgcaggccac gctcccgtcg cccttcgccc
     4501 acgacatacc agtcctcgac ggtcacgcca gcgtggccca ggtcgatcat gccgtcctcc
     4561 gccaccatga tccggtacgc gaacgagtcc gggtccgcca cgggcgggcg gtacggaacg
     4621 gccgtgccgt ggtccggccc ggcggtggcc ggatcgacga cctcgacgcg gaattcgtcc
     4681 aggtccaccc ggtagcggcc gaagccccag tccatgagcg ccagctggcc gtacaccgtg
     4741 tcaccgggcg ccgcatcgaa gccgcgcgtg gtcatcgaaa tggtgtgcca gccgagcgtg
     4801 tccggaatgt cgaactccac caggtgcgaa tggaaatcgg tcgtgcgctg cgtgttcagg
     4861 tgaaggttca cgcggcgcgg ggcgtggctg acgcggatcc gcgcctggat gcgcagttcc
     4921 gcgccctggc tggccaggcg cgaaaggtcc agggaggccg tcacgttgcg cttgacgagc
     4981 gcccaccagg tgttgcggta gtccgctgaa gcgtcgaccc ggatcgacgc ggtggtgtcg
     5041 ccggccacga agtccatcac ggcctcgccg tcgcccgtga accaggtcca cccggaaacg
     5101 tcgcccgagt cgaacgggtc gacgaactgc gcggctgcgg gcgcggaacg cgcagcaacc
     5161 gcgagaatgg ccaggatgac ggagaggcag cgcacgggct cagggtttcg gcagggcgac
     5221 cggtccgtag acggcgtccc ggatggcctt gtgcagggta ccgtcgaacg ggaacacctg
     5281 cacgaaaaag acggccgcga gctcgttcac cgggtccacc cagaagagcg tggtcgcggc
     5341 accgtcccag aagaactcgc ccaccactcc gttgttttca ctggccgagg cgggcgggcg
     5401 cacgcgcacg gcgaagtcga agccgaagcc gacctggccc ttcgacggga gccaggatcg
     5461 ctccgtgacg ctgtcggcga gggcgttcgt ggccatgaga cggacggtct ccggctggag
     5521 gatccggacg ccgtcaagct cgccgccccc gaggagcatg cgggcgaagc gcatgtagtc
     5581 gtcgagggtc gaagtgagcc cgaagccgcc cggcgtgagc ggccaggcct gcatgttgaa
     5641 ggaccggccc agttcgtcgg gtacccggac cagtacgccg tcctcgccgc ggctgaacag
     5701 ggcggcgaat cgccccaggt cactgcccgg gaccacgtag cgggtgtccg tcatgccgag
     5761 cggatcgagc accctctcgc ggacgagctc gccgaacggg cggcccgaga gcctctcggc
     5821 caggtaggcc tggacgtcca ccgacggtcc gtaggcccat ctctcgccgg gatggaacca
     5881 gagcggcagc gtgccgaacc gccgggccat ctcggcgagc gtgttctcgc ggtcgagggg
     5941 ggccgcggcg gtgagaagct cgatcgcctc ggggctcccg gggtccgacg tgatgcccga
     6001 cgtgtgccgg cccaggtcgc ggatcgtcat ctcgcgcctc ggcggttcga gcatcggccg
     6061 gcccgagtcg tccatcccgg cgtatacgcc gagatccgcg aactcgggta cgtgccgcga
     6121 gaccgggtcg tccaggtcga aggccccctc ctcgtacagc gacatgagcg ccgtgccggt
     6181 gatcggcttc gtcatcgaga agatctggac gatcgtgttg cgcgccatgg ggatgccggc
     6241 ctcgcggtcg gcgtgcccga aagccccgaa ataggcctcg cgccccttct cgaagaccaa
     6301 agcagacgct ccggctaccc ggcccgcctc gaccatggcc gacagcgccg agtcgatgcg
     6361 ggcgctcgcc gcgtcgtcga cgacacggtc gaccgcgggc tcggacggcg tgcaggcaga
     6421 gaggaggagg gccgcggcga gggcggcaga cgttgcgcgt gtcatgggtg gatgtggatc
     6481 gtggaatctg ccgcgccgtc agcggaagag cgactcggcc gcatgtgcga cgtagatgaa
     6541 cgtggcgccg agcgggatca cgtactcgcc ctcgtaccag aggaacgggt ggtcctcctt
     6601 gagctcggga aggtcgggct ggacgatgac gatgcccggc acgatgccgc cggggatgaa
     6661 cgaatggtcg gcccggttgg cgccgtaggc cgttgtcatg ctccgcgcgc ccacgccgct
     6721 gaccatcgat acgctggacg ccgggtgcgt gcccaggacg tacgcaaggc cgcgcagcgt
     6781 gtgctccggg gacacgattt ccgggaaggc ccggtgcagg atgtgggcgg tcatcgcgaa
     6841 gccgatcacg gcgcccgagc cgccccagcc gccggtcgtc acgggcacgc cgaacggatt
     6901 cgaggccgcg acctggtcca ggcccgcggc gtaggcgcgc acggcggctt cgacgctccg
     6961 gcggaagccg tcgtccatga acggcaacgc gcgcacagcg agggcggcgt acgcgccgaa
     7021 gcgctgctcc atgccgggcc agagcgcgcc gagccggtcc gcgtacttcc ggtcgccggt
     7081 cgtgaccagg agctcgacgg tcgccttcgc ctcctcgatc tcgaggggtc ctccggtcgt
     7141 gttgccccac cggaagacga ccggctcacg cgcctgctcc tcgtcccata cccgcaccgc
     7201 cgtcgagagg cactcgtcgg cgagcgcgtc gcggtagccg cgcaggacgc gcgaggcggc
     7261 cgccagcgcc gcgatggacc cgtagtcgag cggggtggac cggctcgtga aggcccagcg
     7321 gtcgtcgaac gtgcccgacc ggtggccgtc ggtctcgagg ctgtccagcg acgggtcgta
     7381 gaccaggttg tccgtcatcg tgatcgcgtc gcccaggtgt gtgtactgcg cgatcgtcgg
     7441 gacgatgatc cccgggatgg cgtggcccac cgcgcggtac tgcgcaagca gggcgagcac
     7501 gccgtgctcg acctgctgca cgatgtccgg gaccccgtcc ggcacgccga ggtccacgaa
     7561 acgctcctcc tggcggacgg tggtctggtc ccggtcgatg ccgaacgtct cgcgcgcgag
     7621 gaccagcgcc tggaccgtcg cgtactggct ctgggtgcgg atgtcgtaat cgcccgcatc
     7681 gtgccagccg ccgacatcaa ggccgggaat gtgctcgccc ggctcgaaag gcgaatcggt
     7741 tgcaggaccc tgcgcgtaga ggtcgaaatg ctcgtggtcg acgggggcct ggagggcatc
     7801 gtccaggtgc ccggcgccgt gccacacgcg gtagcggtcg ttgacgagca cgtgatccat
     7861 ctggaccggc atgaaggtgt cgagcgtggg ctgccagact ccggagcggt acacgtcgaa
     7921 gtcgatccgg aagggttcgg agcggacacc cgcgtactcg agcacgtaca gccccggctc
     7981 ctcgacggcc gtgaaatcga acctgcggta ctcgtagcgc aggtacggcc cccacggcag
     8041 ggccggtccg gcgaagcgct cttccgtgcg gccgtccggc aggatccgga gcagccgggc
     8101 cgatgccggc gcctcgaagt gcgggtccag ctccaggacg gcgatcttct cctgccgggg
     8161 atggtatccg acctgcgagt gcgcgaccac cgggcgccgg gtccagtccg ggatccggtg
     8221 cggcgtcacg agccattcca ggacacgccc cgtccgcccg gcgggcagca ggctccgcac
     8281 gacgaaccag ccgttctgcg cctgggtgcg cccgtcgtag agggagaggc gcgcgccgcg
     8341 gccgtcgatc gtcacccgcc gctccggatc cccgggggcg aggacgagcc gtccgcccgt
     8401 cgccatcggc tccgcctcca gcatgccgta cggcgccatc gtccgccgcc cggtcgcgat
     8461 ccgcgggaac gcgccacccg cgtcgtccat catgaaggtc tttccgaaat aggccgacgg
     8521 caggaactcc aggttgaagc ccgcccgccc ctcgagagcg gcggggagcg gcgcgtcgag
     8581 gtggacggcg acgagcgcgc cgcccgcctc cggacggacc tcgatacggt actcgaatcc
     8641 gtggtccggg taggctagcc gcgcggtgac ggctccgctt tccgggtcga cgatccggtc
     8701 cacgagctgc ggaatcggat cccactgctc cggcgtgttg gacaggcgga cgtcgccgtt
     8761 cgtcgccgtc cgcacgccgt ggtggatgag ctcgacgccg ctcagcttgg agtcgctgaa
     8821 gaggccgctg taccagttgc tgaaggcgag gacgtccagg ccccgcgcgg acaggacccc
     8881 gcggtcgtcg agccgcaggt cggtcggctg gccgagagcc gcgggagccg cggcgaggag
     8941 caggacgagg agcgcgggcc cggatcgtgt catggtcggc agggtttcgt tccggcctcg
     9001 ggtaggagga cgggcaagta tgccggtcaa ccgcgcgtta agcaagggcg ccacactttt
     9061 ctcatgatcg gtgcgcgggc ccggtcgccg cgcgtgctgt actattgggc ctgccgccct
     9121 gcggccccgc ccgtgaatcc tgcccgcaga cccatgcgac gcctcgccct cctcttcctt
     9181 ctcgctcccc tcgcctgcag ccccgccgat ccggaggtcg ccgcctggga ggagcgggcc
     9241 tccggcatca cgatcatccg cgacgactgg ggcgtcccgc acgtctacgg ggagaccgac
     9301 gccgacgccg tcttcggcat gatctacgcg caggccgagg acgatttcaa ccggatcgag
     9361 cacaactacc tgctttcgat ggggcgcctg gccgaggccg agggcgaggc ggagatctgg
     9421 cgcgacctcc ggatgaagct cttcatcgac cccgactcca tgcgtgcgct ctacggccgg
     9481 agccccgcgt ggctccggga cctgacggac gcctgggcgg acgggctcaa ctactacctg
     9541 cacacgcatc ccgaggtcac cccgcgggtc atcacgcggt tcgagccctg gatggcgctg
     9601 accttcagcg agggatcgat cggcggggac atcgagcgcg tctcgctccg ggacctggag
     9661 tctttctacg ggcagcgggg gggcagcgcg atcgccggcg agcggcagga tgccgggccg
     9721 ctcgtcccgg ccgacctgta cgccctggcc cgtgagccgc tcgtgccgct ccacgcggag
     9781 cccgtcggct cgaacggctt cgcgatcgcc ccctcgaaca gcgcgacggg caatgcgctg
     9841 ctctggatca acccgcacac gtcgttcttc ttccgcgagg agctccacat ggtgagccgc
     9901 gaaggcctgg acgcgtacgg ggccgtcacg tggggacagt tcttcgtgta ccagggattc
     9961 aacgagggcg cgggctggat gcacacgtcg agcggcgtgg acaacatcga cgagttcctg
    10021 gaagccgtgg tcgagcggga agagggcctc gtctaccgct acggggacgg ggagcggccc
    10081 gtcgtcgagc gcgtgatcac cgtgccctac cgcagcggcg attcgctggc gcaccggacg
    10141 ttcatgtcgt accggaccca ccacggcccc gtggtgcgcg cggagggcga ccgctgggtg
    10201 gccgtgagcc tcatggagga ccccctgggt gcgctcgccc agtcgtactc acggacgaag
    10261 gcccgcaatt acgcggagtt ccgggcgacg atggaaggcc acacgaactc gtcgaacaat
    10321 accgtcttcg ccgattccga gggcaacatc gcgtacttcc actccaactt catcccgcgc
    10381 cgtgacccgc ggttcgacta cgcgcgtccc gtggacggca gcgacccggc cacggactgg
    10441 aacggcgtcc tgtcgatcga
//
LOCUS       DBNHQZ010000179         4871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2356421, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000179 DBNHQZ010000000
VERSION     DBNHQZ010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2356421"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1259)
                     /locus_tag="AC16YU_04695"
     CDS             complement(69..1259)
                     /locus_tag="AC16YU_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6064556.1"
                     /translation="METIDLRGARRLALARAGLLKPRWAGFPVRAAGSGAAARRASLA
                     VVGRFGYLQVDTVSVAGARSHAIVLHSRIEGLDASLAETLLRPDGALFEYWGHEASWI
                     PMSLYPVFAFRRDEYAHHPWWGDVIGEHPRVAAELRLRIREEGPLRSLDLDGANRGGW
                     WNLKVANRVASALWLSGELAIRERVRFQRTYDLAERVIPPEFLGRAVPGEDALETLLL
                     LALQGHGWAPTGTLVRTWRLSNRPEAIRAALRRLQERGAVIPCRLEDTGAGGWIRPGD
                     LDLAERLRGLRPRADRGVLLSPFDPLLWDRRRVRTLFGFDQVLEIFKPQPKRVYGYYC
                     LPVLAGEHLVARVDLKADRRAGTVRVLSCHVEERARRGHVEAVRSALARFGASVGLEP
                     VGGP"
     gene            1533..1949
                     /locus_tag="AC16YU_04700"
                     /pseudo
     CDS             1533..1949
                     /locus_tag="AC16YU_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017117222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(2031..2825)
                     /locus_tag="AC16YU_04705"
     CDS             complement(2031..2825)
                     /locus_tag="AC16YU_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6064557.1"
                     /translation="MNPFTTHGAISWPELMAVDADAAADFYARIFGWSVESMPMASGD
                     YHVASSAGVNRAGMMDRPSDEIPPSWIFYVTVRDVHAITERAIQLGASIEVPPMEVPG
                     VGAFAGIVDPQGAYIFVMQYEETSAGGEPPPVADFLSFFATEGAFSWFQLLTPDPAAS
                     ARFYADLFGWETEEQAQPMGPYHVVKIGEIGFGGITAPFTPDIPPHWQGYVTVDDADE
                     TAARVVDAGGQILMPPTDVPGVGRMLALQDPQGAMLMAVAYLVPGA"
     gene            complement(2870..3394)
                     /locus_tag="AC16YU_04710"
     CDS             complement(2870..3394)
                     /locus_tag="AC16YU_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6064558.1"
                     /translation="MDAFRRPSPDEYNTHYAGYVRTVPDGPILDFLEVRRDELADLLR
                     SVPPDLETYRYAPGKWSVREVAGHVIDSERVFAYRALAFARGASDPLPGMDQNAYAAA
                     APFGDRTLASIADEFVTVRDATISLVRSFGPGIADRGGIASGYPVTVRALVWIIAGHE
                     RHHAYVLADRYLTR"
     gene            complement(3401..3991)
                     /locus_tag="AC16YU_04715"
     CDS             complement(3401..3991)
                     /locus_tag="AC16YU_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064559.1"
                     /translation="MADIRLRPRFRRPVRCTPRAVLGAIGRAIGGPGAPVAGTVFESS
                     AVLSVLPDRQHFWSPQLQLSVEADGDGADVHGLFGPHPTVWSLFVATYVAIGFLGSMG
                     VTFGFAQWTMGRSPWALWSAPLAAVLALAVWIVGRAGRRLGMAQVRELFAFVEATLDA
                     CEEPRVPGGTDDDAPSGAHTANPDGARSSNPPGSPA"
     gene            complement(4043..4633)
                     /locus_tag="AC16YU_04720"
     CDS             complement(4043..4633)
                     /locus_tag="AC16YU_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HIR6064560.1"
                     /translation="MIRSRRLAALVAGLILAGCAESTSRQPAPSAAQVQPADPARETV
                     QTAGIGSIPNLTRLGDLYFAGQPSAGDLALLPDAGVTTVISLRHEAELGGFDEKSAVQ
                     DLGLQYLEVPWNGPAELTDDVFDRTRAMLLEVDSPTLFHCGSANRVGAVWLPYRVLDE
                     GVPVEQAVEEARKIGMRTPEYEARALDYIARNLESR"
     gene            4697..>4871
                     /locus_tag="AC16YU_04725"
     CDS             4697..>4871
                     /locus_tag="AC16YU_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIR6064561.1"
                     /translation="MRDLDPIRPRAPVLVEACVTTVREAVASAAAGALRLELCIDLEE
                     GGLTPPPDLVREVV"
BASE COUNT          733 a         1694 c         1794 g          650 t
ORIGIN      
        1 ctaccggagc ggggaccgct gggtgctttc gaaagtcatg gactagggcc cgcaggccct
       61 ggcgccgttc acgggccgcc gaccggctcc aggcccaccg aggcgccgaa gcgcgcgagc
      121 gccgaacgga cggcctccac gtggccgcgg cgggcccgct cctcgacgtg gcacgagagc
      181 acgcgcaccg ttccggccct ccggtcggcc ttgaggtcca cccgcgccac caggtgctct
      241 cccgcgagga ccggcaggca gtagtacccg tacacgcgct tcggctgcgg cttgaaaatc
      301 tcgagcacct ggtcgaagcc gaaaagcgtc cggacgcgcc gccggtccca gagcagcgga
      361 tcgaacgggg agagcagcac gccgcggtcg gcgcggggcc gaaggccgcg caggcgctcg
      421 gcgaggtcga ggtcgcccgg ccggatccag cccccggcgc ccgtgtcctc gagccggcac
      481 ggaatgacgg caccccgctc ctggagtcgc cgaagggcgg cacggatcgc ctcgggacgg
      541 ttggacaggc gccacgttcg caccagcgtg cccgtgggcg cccacccgtg gccctgcagg
      601 gcgaggagca ggagggtctc gagtgcgtcc tccccgggaa ccgcacggcc aaggaattcg
      661 ggcgggatga cccgttcggc gagatcgtac gtgcgctgga agcgaacgcg ctcgcggatg
      721 gcgagctcgc cggacagcca gagcgccgac gcgacccggt tcgcgacctt caggttccac
      781 cacccgcccc ggttggcccc gtccaggtcc agcgagcgga gcgggccttc ctcgcggatg
      841 cgcagccgca gctcggccgc cacccgcgga tgctcgccga tcacgtcgcc ccaccacggg
      901 tggtgcgcgt actcgtcgcg gcggaaggcg aagaccgggt acagggacat gggaatccag
      961 ctggcctcgt gcccccagta ctcgaagagc gctccgtcag gccgcaggag cgtctcggca
     1021 agcgaggcat ccaggccctc gatgcgcgag tgcagcacga tggcgtgcga gcgcgccccg
     1081 gccacggaga cggtgtcgac ctgcaggtag ccgaaccgcc cgacgaccgc gagggaggcg
     1141 cggcgggcgg ccgcgcccga gcccgcggcg cggaccggaa acccggccca tcggggcttc
     1201 aggaggccgg cgcgggccag ggccagccgg cgcgctccgc ggaggtcgat cgtttccatg
     1261 acccgtgaga atcgcgtatg ggacgttgtc ccgcaacctg ggccgggccg atcgcaggag
     1321 gtgacgtata cttgtacctt gaacgaatac cttcatcgag gtccctcatg ccacgtcatc
     1381 tttccgtcgc cctgctctac gcccttgcac tcgggttgtg cgcctgcggc ggctcggcgg
     1441 accgggagca agccgccgag gaggccgaag ccgccgccgc cgatgccccg gccgatgccg
     1501 cgatgactgg cacatcgccc tgcttcctcc gggccgtcga agcgtccgca tccggagcca
     1561 ccgcgaggga gcgcggggag ctcttcctgg ccgccaacgg acagtgcgag ggcgtgatgc
     1621 agacggcctc cggtctccag tacgaggtca tcgtgccggg tgacggcccg cggccgacgg
     1681 ccacctccac ggtgaccgtc aactacgagg gctcgctcct caacgggtcg gtgttcgaca
     1741 gctcctacga acggggcgag ccgatctcgt tcccgctcga ccgcgtgatc gccggctgga
     1801 ccgagggcgt cgcgctcatg cccgtcggct ccacctaccg gctgtacatc ccgtcgggcc
     1861 tggcctacgg cccgagcggc tcggggcagg tcatcgggcc ggacgagacg ctcgtattca
     1921 acgtggagct cctgggcatc gtcgagtagg cgcgcgacaa gcccggatcg tctggacgcg
     1981 gcggcgtcca ctcttcgtac gcacgggcct gacggcctcg aagccggtcg tcaggcgccg
     2041 ggcacgaggt aggcgaccgc catgagcatg gcgccctgcg gatcctgcag ggcgagcatg
     2101 cgccccaccc cgggcacgtc ggtcggcggc atcaggatct gtcctccggc gtccacgacg
     2161 cgcgccgccg tctcgtcggc atcgtcgacg gtcacgtaac cctgccagtg gggcgggatg
     2221 tcgggcgtga agggcgccgt gattccgccg aagccgatct cgccgatctt cacgacatgg
     2281 tagggaccca tgggctgggc ctgctcctcg gtttcccagc cgaagaggtc ggcgtagaac
     2341 cgggccgagg ccgccggatc gggcgtgagc agctggaacc agctgaacgc gccctctgtc
     2401 gcgaagaacg agaggaagtc ggcgaccggc ggcggctccc cgccggcgga cgtctcttcg
     2461 tactgcatga cgaagatgta agcaccctgt gggtcaacta tgccggcgaa tgcccccacg
     2521 ccggggactt ccatcggcgg gacctcgatc gaagcgccca gctggatcgc ccgctcggtg
     2581 atggcgtgca cgtcccggac cgtgacgtag aagatccagc tcggcgggat ctcgtccgag
     2641 gggcgatcca tcatgccggc acgattcaca cccgcgctgc tggccacgtg gtagtcgccg
     2701 gatgccatgg gcatgctttc gaccgaccag ccgaatatcc gggcatagaa gtcggcggcg
     2761 gcgtccgcat cgacggccat gagttcgggc cagctgatgg ctccgtgggt ggtgaagggg
     2821 ttcatggtcg cggggcgggt ctgtgtcggt ttgagggttc gggccggccc taccgggtga
     2881 ggtagcggtc ggcaagcacg taggcatggt gccgctcgtg cccggcgatg atccagacga
     2941 gggcgcgcac ggtcacgggg tatccggacg cgatgccgcc gcggtcggca atcccgggcc
     3001 cgaacgaccg caccaggctg atggtggcgt cccggacggt gacgaattcg tccgcgatcg
     3061 aggcgagcgt ccggtccccg aagggcgcgg cggcggcgta cgcgttctgg tccattcccg
     3121 gcaggggatc cgaggcgccg cgggcgaagg ccagggcgcg gtaggcgaag acgcgctccg
     3181 agtcgatgac atggccggcc acctcccgca cgctccactt gcctggcgcg taccggtagg
     3241 tctcgagatc cggagggacc gaccgcagca ggtcggcgag ctcgtcgcgg cggacctcga
     3301 ggaaatccag gatcgggccg tccgggacgg tgcggacgta acccgcgtaa tgggtgttgt
     3361 actcgtcggg agagggtcgg cggaaggcgt ccatgggtct ctatgccggt gatcctggcg
     3421 ggttggacga gcgggcgccg tcggggttcg cggtgtgcgc cccggacggc gcgtcgtcgt
     3481 cggtcccgcc cggtacccgc ggttcctcgc acgcgtcgag cgtggcctcg acgaaggcga
     3541 agagctcgcg cacctgggcc atgcccaggc ggcgtccggc gcgcccgacg atccacacgg
     3601 cgagcgccag cacggcggcc aggggcgccg accagagggc ccacggcgac cgccccatgg
     3661 tccactgcgc gaagccgaac gtcacgccca tcgagcccag gaagccgatc gccacgtagg
     3721 tcgcgacgaa cagggaccag actgtgggat gcgggccgaa caggccgtgc acatcggctc
     3781 cgtcaccgtc cgcctcgacc gagagctgca gctgcgggga ccagaagtgc tgccggtcgg
     3841 ggagcacact caggacggca gacgactcga agaccgtgcc ggctacgggc gcgccgggtc
     3901 cgccgatcgc gcggccgatc gcacccagca ccgcccgcgg cgtgcaccgg acggggcggc
     3961 ggaaccgcgg gcgcagtcgg atgtcggcca tgcgcgccgg ggatctaccg ccgcaccgag
     4021 gagccgcgga ccacgggccc cgtcaccggg actcgaggtt tctcgcgatg tagtccagcg
     4081 cgcgggcctc gtactccggg gtccgcatgc cgatcttccg cgcctcctcg accgcctgtt
     4141 ccacgggcac gccctcgtcc agaacgcggt acgggagcca cacggccccg acgcggttcg
     4201 cggagccgca gtggaagagg gtcggcgaat ccacctcgag aagcatcgcc cgtgtcctgt
     4261 cgaagacgtc gtccgtcagc tccgcggggc cgttccaggg gacttccagg tactggaggc
     4321 ccaggtcctg cacggcggac ttttcgtcga acccgccgag ctccgcctcg tgccgaaggc
     4381 tgatcaccgt ggtgacgccc gcatccggga ggagggccag gtcgccggcc gacggctggc
     4441 cggcgaagta caggtccccc agccgggtca ggttcgggat gctgccgatc ccggcggtct
     4501 gcacggtctc gcgggcggga tcggccggct ggacctgagc cgccgaaggc gcgggctgcc
     4561 gggaggtgga ctccgcgcag ccggcgagga tgaggccggc gacgagagcg gcgaggcggc
     4621 gggatcggat catgaacgcg gcgtgaaatg gtggggccat accaaccgcg gtagccccgc
     4681 ggcgttccga agcgtcatgc gcgacctcga tccgatacgg ccgcgggccc ccgtgctcgt
     4741 cgaagcgtgc gtgaccacgg tgcgcgaggc cgtggcgagc gcggcggccg gagcgctccg
     4801 gctggagctg tgcatcgacc tggaggaggg cggcctcacg ccgccccccg acctggtgcg
     4861 cgaggtcgtg c
//
LOCUS       DBNHQZ010000180         8366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1137141, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000180 DBNHQZ010000000
VERSION     DBNHQZ010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1137141"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="AC16YU_04730"
     CDS             <1..1048
                     /locus_tag="AC16YU_04730"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR6064562.1"
                     /translation="GAVTPVLMVAVRLALFAALRRARRLGHYRRRVLIVGAGNVGRRL
                     GKAITEYPWMGFELVGYLDDFKTDQDVLGPTSDAARIVDAFEAEGAPVDYLYVALPLT
                     AAPRIEQVVGEMTTRLVHVCLVPDLFQFDILNGRVTELDGLPVIHLIDETPLDFRRAV
                     KRGIDIVFSAAFLVLVSPLLLVVAVAVKLSSPGPVFYRQERMGLNGQRFDMLKFRSMP
                     VDAEAETGAVWAQPGERRATRVGSFLRRTSLDELPQFINVLRGDMSVVGPRPERPVFI
                     QEFRDRVPRYMLRHKMKAGITGWAQVNGWRGDTSIERRIEHDIYYIRNWSLRLDLKIM
                     LMTQSRGFVNENAY"
     gene            1121..3082
                     /locus_tag="AC16YU_04735"
     CDS             1121..3082
                     /locus_tag="AC16YU_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6064563.1"
                     /translation="MSWTIRDRVRVGLVGPNGAGKTTLLRVVAGLLDVDEGTVQAGGS
                     TLGYLRQEVEETQGHRTVVEEAMDAFAAIHTLHEEEDRILSSLERETDHTSARYARLL
                     EDLDQVHQRLHATEAHTARPRAEAVLSGLGFGAKDMDEPVSTFSGGWRMRVELAKLLL
                     SRPDVLLLDEPTNHLDIDSIDWLETYLKTYPGTVVIVSHDRYFLDRMVNRIAELSRGR
                     VTEYAGNYAFYLKERAERRELQRASFVNQQKAIAETERFIERFRYKNTKATQVQSRIK
                     ALGRLERITEPEPDEAAISFRFPEPPRSGRMVLELSRFSKTYVDEGRSVPVFREAGPL
                     QIERGDKIALIGRNGAGKSTLARMLAGTEPFDGTRRLGKDVELTFFAQHTADTLDVDA
                     TVLEAVQRVAFGRSETEVRSLLGAFLFRGDDVFKPIRVLSGGEKSRVALATTLVVPAN
                     LLVLDEPTNHLDIHSIAVLIEALRQFTGTFAVVSHDRHFLDQVVTRVWRVEDGTVRVF
                     PGTYSEYLWKVGQETKPVVVARAKADAEPRSAHPRTAAAQVSAVPDAAVPDAAAPREP
                     APADPENPFRTLNTFRLKRALVEAEREIQEHEDRKSSLEAALGDPSLYADADRPRETT
                     EAYDAVQRELAELYRLWEAIAEAVAERGA"
     gene            3138..4337
                     /locus_tag="AC16YU_04740"
     CDS             3138..4337
                     /locus_tag="AC16YU_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR6064564.1"
                     /translation="MHRPNAPLLSRTLVAASIVWGVSAAAPCPAQIHRLPSPDSTAGN
                     HFGTAVAIDGDRIVVGATGADVCGPNSGAAYVYERSGEFTWRLEAVLQPEDCEEQDFF
                     GRAVAVAGDRIAVTSYRPYFSTFRSNAVHLFERDEDGNWERASRIEAPGGAAEGAFAA
                     SIALGEDRFVVTSLGETRPDGHRGGATVYERGTDGAWVVAARLEGDADGRAFGASCAL
                     DGEFAAVSASSASRRVAGTVLVFEREADGRWVRRATFSGIRDFFIPMALDAGRLLVGQ
                     RDGGHSRSGVATLYEPGPDGAWHRTARLRPPVPYPSGGFGSGISLTGEHALVVGFDEQ
                     LQFDFNIDRVVYVFSPDESGAWSQRQVIDLGETHFGSAIDADGALAVIGSAADGRPGA
                     AYVVGLR"
     gene            complement(4350..5221)
                     /locus_tag="AC16YU_04745"
                     /pseudo
     CDS             complement(4350..5221)
                     /locus_tag="AC16YU_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
     gene            5371..7749
                     /locus_tag="AC16YU_04750"
     CDS             5371..7749
                     /locus_tag="AC16YU_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064565.1"
                     /translation="MRNLTFSLLLGALGLAALPVSAQVATVRRPVDLGTKRVEAPVDG
                     QRILHVTDRLMALPESRAALEAFHIRKEAGLLPVAGKSEAAVGTMQTFNVRNISTNVF
                     EPIDFTLVLSRARFDIWVETAQYPDSVSAGVIEELRAALEDETPFGSVNPAAGIIVND
                     EVYFGDPPNSDGDGRTDIMVLDVRDDCAETDVCVLGFFDPVDLSIASPRDAVYLDINP
                     GLTSFGVQGVVQTAAHEYQHLIHAGYDANELTFVNEGLSEWAEVLNGYRARSITYLDD
                     LVEQRLPLMHWAPSNEIGSVQDYQRAGLLTNYIAEQIGPVATGSITRTAADGAAGYQA
                     VLTPLALDLTELVRDFHVANHVNNRVIRAEYGYATSQYANLSAAIRSSHRGSESTEIL
                     PTTHGPLLPGAVDYHTIEYVEDLSLRLYVPVGAADPAAVVARIRPVVVLFLDDGSVEV
                     HEVTIQDEARSYPQIIRKAVVVVPHAQLASPSSSLSYGIEASWTPAQVSFQTEAVTYD
                     AGMLDGGNIFGISTSGRGANRFDVAAGSILSSVSVSVMFEHDFDQSPVPPSAARDYRL
                     SVWAEGTTPGVPAATPLFTLDRVDSRSVSLVTNGVDTLFVFDPINLESYQAELSGLPG
                     TVFVSVENVGADENYLYYPVSEYDPTAQDSPSLFFNTAQTIPGWRRFTQLQSGELSLN
                     ERVLPIRATFLVPVANEGEADLPEAYALEPNWPNPFNPSTTIAYRLPSAGRVRLAVFD
                     VLGRQVATLVDGVMPAGRHETRFDATGLASGLYLYTLDAGSGRISRPMILVK"
     gene            complement(7750..8061)
                     /locus_tag="AC16YU_04755"
     CDS             complement(7750..8061)
                     /locus_tag="AC16YU_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064566.1"
                     /translation="MTSPLKILPLALLLSLAACSAPRPVQPPVTVEGRVAFRGNEPFA
                     AAWLETDMRTYYVLVMDEATEAALVTPARYSVTGRVYRDDWNGMPLAHVAVESMRRID
                     G"
     gene            8060..>8366
                     /locus_tag="AC16YU_04760"
     CDS             8060..>8366
                     /locus_tag="AC16YU_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064567.1"
                     /translation="MDSGSGAARSPARPRRRIDSGRRILAVLLLFAVPTLETRAQDLR
                     QHAWCGTDLSSDEARAALAAYATARDAGLLDVARKTMAPPVLGEVRSFNVLDGPDLNT
                     "
BASE COUNT         1254 a         2963 c         2839 g         1310 t
ORIGIN      
        1 cggcgcggtc acacccgtgc tgatggtcgc cgtgcggctc gctctcttcg ccgcgcttcg
       61 ccgggcccgt cgcctgggcc actaccgccg ccgggtcctg atcgtgggag cgggcaacgt
      121 ggggcgccga ctgggcaagg ccatcaccga gtacccgtgg atgggcttcg agctggtcgg
      181 ctacctcgat gacttcaaga cggaccagga cgtcctcggg cccacctccg acgccgcccg
      241 catcgtggac gctttcgagg cggagggtgc ccccgtcgac tacctgtacg tggccctgcc
      301 gctgaccgcc gccccccgga tcgagcaggt cgtcggcgag atgaccaccc gtctcgtgca
      361 cgtgtgcctc gtgcccgacc tgttccagtt cgacatcctg aacggccgcg tcaccgagct
      421 cgacggcctg ccggtgatcc acctgatcga cgagacgccg ctcgatttcc gccgcgcggt
      481 caagcgggga atcgacatcg tgttctctgc ggccttcctc gtgcttgtct cgccgcttct
      541 cctcgtcgtg gcggtcgccg tgaagctctc ctcgccaggg ccggtcttct accgccagga
      601 gcggatggga ctgaacggcc agcgcttcga catgctcaag ttccgctcga tgccggtgga
      661 cgcggaggcg gagaccggtg ccgtctgggc ccagccgggc gagcggcgcg caacccgcgt
      721 cggctcgttc ctccgcagga cctcgctcga cgagcttccg cagttcatca acgtcctgcg
      781 cggcgacatg tcggtcgtcg gcccgcgccc cgagcggccg gtcttcatcc aggaattccg
      841 tgaccgcgtt ccgcgctaca tgctgcgcca caagatgaag gccggcatca cggggtgggc
      901 gcaggtgaac ggatggcggg gcgacacatc gatcgagcgc cggatcgagc acgacatcta
      961 ctacatccgc aactggtcgc tgcgcctcga tctcaagatc atgctgatga cccagtcgcg
     1021 cgggttcgtg aacgagaacg cgtactgaac cgcgtccacg gcgatcatcc agctccacgg
     1081 catatcgctc gccttcggcg gccaggtcgt cctggaccgg gtgtcctgga ccatccgcga
     1141 ccgggtccgc gtgggcctcg tgggaccgaa cggggccggg aagaccacgc ttctccgggt
     1201 cgtggccgga cttctggacg tggacgaggg taccgtccag gccgggggct cgacgctcgg
     1261 ctacctgcgc caggaggtcg aggagaccca gggccaccgg accgtggtcg aggaggcgat
     1321 ggacgccttc gcggccatcc ataccctgca cgaggaggag gaccgcatcc tctcctctct
     1381 ggagcgggag acggaccaca cctccgcccg ctacgcgcgc ctgctcgagg acctggacca
     1441 ggtgcaccag cggcttcacg cgacggaggc gcacacggcg cggccgcgcg ccgaggccgt
     1501 cctttccggc ctcggcttcg gggcgaagga catggacgag ccggtctcga cgttttcggg
     1561 cggctggcgc atgcgcgtgg agctggcgaa gctgctcctc agccggccgg acgtcctgct
     1621 cctggacgag cccacgaacc acctggacat cgactcgatc gactggctcg aaacgtacct
     1681 caagacgtat ccgggcacgg tcgtcatcgt ctcgcacgac cgctacttcc tggatcgcat
     1741 ggtgaaccgg atcgccgagc tctctcgggg acgggtcacc gagtacgccg gcaattacgc
     1801 tttctacctg aaggagcggg ccgagcgtcg cgagctgcag cgggcctcgt tcgtgaacca
     1861 gcagaaggcc attgccgaga cggagcgctt catcgagcgc ttccggtaca agaacaccaa
     1921 ggccacccag gtgcagagcc ggatcaaggc gctcggccgg ctcgagcgga tcaccgagcc
     1981 ggagccggac gaggccgcga tctcgttccg cttcccggag cccccgcgct ccgggcgcat
     2041 ggtactggaa ctgtcgcggt tctcgaaaac gtacgtggac gaggggcgaa gcgtcccggt
     2101 cttccgggag gccggccccc tgcagatcga gcggggggac aagatcgccc tcatcgggcg
     2161 caacggcgcg ggcaagtcca cgctcgcccg catgctcgcc ggcacggagc ccttcgacgg
     2221 cacacgccgg ctcggaaagg acgtggagct cacgttcttc gcacagcaca cggccgacac
     2281 gctcgacgtc gacgcgaccg tgctcgaagc cgtgcagcgg gtggccttcg ggcggagcga
     2341 gaccgaggtc cgctcgctgc tcggggcctt cctgttcagg ggcgacgacg tgttcaagcc
     2401 catccgggta ctctcgggcg gcgagaagag ccgcgtcgcg ctcgccacga ccctggtcgt
     2461 ccccgcgaac ctcctggtcc tggacgagcc gaccaaccac ctggacatcc actcgatcgc
     2521 ggtcctgatc gaggcgctga ggcagttcac cgggacgttc gccgtggtga gccacgaccg
     2581 ccatttcctg gaccaggtgg tcacccgcgt ctggcgcgtc gaggacggca cagtccgggt
     2641 ttttccgggc acgtattccg agtacctgtg gaaggtgggg caggagacga agcccgtcgt
     2701 cgtcgcacgg gcgaaggccg acgcggagcc ccggtccgcc caccctcgga ccgctgcagc
     2761 gcaggtttcc gccgtgccgg atgccgccgt gccggatgcc gccgcgcccc gcgagccggc
     2821 gcccgccgat ccggagaatc cgttccggac gctgaacacg ttccgcctga agcgggccct
     2881 cgtggaggcc gagcgggaaa tccaggagca cgaggaccgg aagtcctcgc tcgaggccgc
     2941 gctcggcgac ccttcgctct acgcggatgc cgaccggccg cgggagacga ccgaggcgta
     3001 cgacgccgtc caacgcgagc tggccgagct gtaccgcctg tgggaggcca tcgccgaggc
     3061 cgtcgcggag cgcggagcgt gaacggatcc cgtggcggtc tgtccggtac cacgggctga
     3121 acgtggaacc cggcccagtg catcgaccaa acgcccccct gctcagccgg accctggttg
     3181 ccgcgtccat cgtctggggg gtatcggcgg cggctccctg cccggcgcag atccaccgcc
     3241 tgccgtcccc cgacagcacg gcgggcaacc atttcgggac ggccgtcgcc atcgacgggg
     3301 accggatcgt ggtgggcgcc acgggcgccg acgtgtgcgg cccgaattcc ggagcggcgt
     3361 acgtctacga gcgctcgggg gaattcacct ggcgcctcga ggccgtcctg cagcccgaag
     3421 actgcgagga gcaggacttc ttcggccgcg ccgtggccgt cgcgggggac cggatcgcgg
     3481 tcacgtccta ccggccctat ttcagcacgt tccggtcgaa cgcggtgcac ctgttcgagc
     3541 gggacgagga cggcaactgg gagcgcgcga gccgcatcga ggcgcccggc ggtgcggccg
     3601 agggagcgtt cgcggcttcg atcgcgctcg gggaggaccg cttcgtcgtg acgagcctcg
     3661 gcgaaactcg gccggacggg caccgaggcg gcgccaccgt ctacgagcgc ggcacggacg
     3721 gggcgtgggt cgtggccgcg cgcctcgagg gcgatgccga cggccgtgcg ttcggggcct
     3781 cgtgtgcgct ggacggcgag ttcgcggccg tgtccgcatc ctcggcttcg cgccgcgtcg
     3841 caggcacggt cctcgtcttc gagcgcgaag cggacggcag gtgggtgcgc cgggcgacgt
     3901 tctccggcat ccgggacttc ttcatcccga tggccctcga cgccggtcgg ctcctggtcg
     3961 gccagcgcga cggcggccac agccgaagcg gtgtcgccac gctgtacgag cccgggccgg
     4021 acggcgcgtg gcaccgcacc gcccgactgc gcccgcccgt tccttatccg tcgggggggt
     4081 tcggctccgg gatctcgctc acgggcgagc atgcgctggt ggtcggcttc gacgagcagc
     4141 tgcagttcga tttcaacatc gaccgcgtcg tgtacgtctt ctctccggac gagtccggcg
     4201 cctggtctca gcgccaggtc atcgacctcg gggaaacgca cttcggctcg gccatcgacg
     4261 cagacggtgc cctggccgtg atcggttccg cggcagacgg ccgacccggc gccgcctacg
     4321 tcgtggggct gcgctgaccg tcggccccgt cagaaacgcg gggtgtagac gtggcaatac
     4381 ggcgaatgcc cggtcttcgc gtagtagtcc tggtgatagt cctcggcagg ccagaacgtc
     4441 ccggcaggca ccacctcggt cgccacgtcc agccccttct gcccgagcag atccacgagc
     4501 cgtaccgccg tctcgcgctg cgcctcgtcc agcgtgaaga tcgccgaacg gtactgggtc
     4561 cccacgtcag ggccctggcg atccacctgg gtggggtcgt gggtctcgaa gaagaggcgg
     4621 gcgagcgtct cgtattcggt gaccgacggg tcgaaggtca cttcgacggc ctccgcgtgc
     4681 cccgtgcggc ccgtgcacac ctcccggtaa gtcggccgct ccacgtggcc gccgatgtat
     4741 ccgacacgcg tcgacagcac gccttcggcc cgtttcatgt agtactccgt gccccagaag
     4801 cagcccgagg cgaagatggc gcgttccggc tccgtcatga ccgtcacccc tccccggggc
     4861 cgcccgcggg ccggaaatcc agggacagcg agttgacgca gtgccggacg tcccgggggg
     4921 tgaatccctc cccctcgaag acgtggccca ggtgggcgcc gcaccgggcg cagacgatct
     4981 ccgtgcgacg gccgtccggg tccggtcgcc tctcgacggc accgtcgatc gcgtcgtcga
     5041 agctgggcca gccgcagtgg gcgtcgaact tgtcgtccga atggtagagc ggcgcgtcgc
     5101 agcgtcggca caggtaggtg ccggactcct cgtgccggta gaagcgcccc gagtacggcg
     5161 gctcggtgcc gcggtgcagg atcacccgct cctcggccgg cgtcaggtca ttgagcttca
     5221 tgggggtaga cgctgggtcc cggggtatga tgtcgagggc gaatcggttc ccggctgtgc
     5281 gtccgaggaa acggcatccc gcgcgcggtg tagaagcggc tcgcgtcgac cgatactacc
     5341 cccgtctcgc cagaagccga tcatccgaac atgcggaacc tcacgttttc actcctcctg
     5401 ggcgccctcg gtctcgccgc cctgcccgtc tcggcccagg tggccacggt ccgacgcccc
     5461 gtggatctcg ggaccaaacg cgtcgaggcc ccggtcgacg ggcagcggat cctgcacgtc
     5521 acggatcggc tcatggccct tcccgagtcc cgcgccgcgc tggaggcttt tcacatccgg
     5581 aaggaagccg gtctcctgcc ggtggccggc aagtcggagg ccgcggtggg cacgatgcag
     5641 accttcaacg ttcggaacat ctcgacgaac gtgttcgagc ccatcgattt cacgctggtc
     5701 ctgtccaggg cccgcttcga catatgggtc gagaccgcac agtatccgga ttccgtgtcg
     5761 gccggcgtga tcgaggagct ccgggccgcg ctcgaggacg agacgccgtt cggatcggtc
     5821 aacccggccg cgggcatcat tgtgaacgac gaggtctatt tcggcgaccc gcccaattcg
     5881 gacggagacg gcaggacgga catcatggtg ctcgacgtgc gggatgactg cgcggagacc
     5941 gacgtgtgtg tcctgggctt cttcgatccc gtggacctca gcatcgcgtc gccgcgcgac
     6001 gcagtgtacc tggacatcaa ccccggcctc acgtcgttcg gcgtgcaggg cgtggtccag
     6061 accgccgcgc acgagtacca gcacctgatc cacgcgggat acgacgcgaa cgaactcact
     6121 ttcgtgaacg agggtctctc cgagtgggcc gaggtgctga acgggtaccg cgcccgctcg
     6181 atcacgtacc tcgacgacct cgtggaacag cgcctgccgc tcatgcactg ggccccctcg
     6241 aacgagatcg gatccgtgca ggattaccag cgcgcgggac tcctgaccaa ttacatcgcg
     6301 gagcagatcg gacccgtcgc aacgggttcg atcacgcgga cggccgcgga tggcgccgcg
     6361 gggtaccagg ccgtgctgac gccactggcg ctggacctga cggagctcgt ccgcgacttc
     6421 cacgtggcga accacgtgaa caaccgggtg atcagggccg agtacggcta cgccacgtcc
     6481 cagtacgcca atctctcggc cgccatccgc tcttcgcacc ggggctccga gtccaccgag
     6541 atcctgccca cgacccacgg accgctcctt ccgggggcgg tcgattacca caccatcgaa
     6601 tacgtcgagg acctgagcct gcggctgtac gtccccgtcg gagcggccga tccggccgcc
     6661 gtggtcgcca ggatccggcc cgtggtggtg ctcttcctgg acgacggctc cgtcgaagtc
     6721 cacgaggtca cgatccagga cgaggcgcgc agctacccgc agatcatccg gaaggcggtc
     6781 gtcgtggttc cccacgcgca gctcgcgtcg ccttcatcgt cgctcagcta cggcatcgag
     6841 gcctcgtgga cgccggccca ggtgagcttc cagacggagg ctgtcaccta cgacgcgggc
     6901 atgctcgacg gggggaatat cttcggcatc tccacgagcg gacggggcgc aaaccggttc
     6961 gacgtcgccg cgggaagcat tctctcgtcc gtgtcggtgt ccgtgatgtt cgagcacgac
     7021 ttcgaccaga gcccggtgcc gccctccgcc gcgcgcgact accgcctgtc cgtgtgggcc
     7081 gagggcacga cgccgggggt tccggcagcc accccgctct tcacgctcga ccgcgtggac
     7141 tcgcgctcgg tgagcctggt cacgaacggc gtggacacgc tcttcgtctt cgatccgatc
     7201 aacctggaaa gctaccaggc cgagctttcg ggcctccccg gcaccgtgtt cgtctccgtc
     7261 gagaacgtgg gcgcggacga gaactatctc tactatcccg tctcggagta tgacccgacg
     7321 gcccaggatt cgccctcgct cttcttcaac acggcccaga ccatcccggg ctggcgccgt
     7381 ttcacccagc tccagtcggg cgaactgtcg ctgaacgagc gcgtcctccc catccgggcc
     7441 accttcctcg tgccggtcgc gaacgagggc gaggcggacc tgcccgaggc gtacgcgctg
     7501 gagcccaact ggcccaaccc gttcaaccct tcgaccacga tcgcgtaccg cctgccgtcg
     7561 gccgggcgcg tgcgtctcgc ggtattcgac gtcctgggcc ggcaagtcgc cacgctcgtc
     7621 gacggcgtga tgccggccgg ccggcacgag acccggttcg acgcgaccgg actggcgagc
     7681 gggctctacc tgtatacact ggacgcggga tccgggagga tctcccgccc catgatcctc
     7741 gtgaaatagc tacccgtcga tgcgccgcat ggactcgacc gcgacgtgcg ccagcggcat
     7801 gccgttccag tcgtcccggt acacccggcc ggtcacactg taccgggcgg gcgtgacgag
     7861 cgcggcctcc gtggcctcgt ccatcaccag cacgtaatac gtccgcatgt cggtctccag
     7921 ccacgccgcc gcgaacggct cgttgccccg gaaggcgacg cgtccctcca cggttacggg
     7981 aggctgcacg ggccgcggag ccgagcaggc cgcgagagat agcagcaggg cgagcgggag
     8041 gatcttcagc ggagaggtca tggattccgg gagcggggcg gcgcgttctc ctgcacgccc
     8101 ccggcgccgg attgattccg gccgcaggat cctcgcggtc ctcctgctct ttgcggtccc
     8161 gaccctcgag actcgtgccc aggacctgcg gcagcacgcg tggtgcggca ccgacctgtc
     8221 ctccgacgag gcgcgcgcgg ccctggctgc ctacgcgacg gcccgcgacg cgggcctcct
     8281 ggacgtggcc cggaagacga tggcgccgcc cgtcctcggc gaggtccgct cgttcaacgt
     8341 gctcgacggc ccggacctga acaccg
//
LOCUS       DBNHQZ010000181        13876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1004094, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000181 DBNHQZ010000000
VERSION     DBNHQZ010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13876
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1004094"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="AC16YU_04765"
     CDS             complement(<1..895)
                     /locus_tag="AC16YU_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR6064568.1"
                     /translation="MSRVIRSRAPLRISFAGGGTDVSPYAEERGGRVLNAAINRYAYV
                     TLAPNGSDQIRMQSLDLGEEVAFRTDAPVPDVDYQMSLAKGVVRRMTEGRRLPGFDLF
                     THADCPPGSGLGASSTMVVALVGAFDAWLGLGLSRYETADLAYEIERIDLGIAGGRQD
                     QYAAAFGGFNYLEFGPGPALVTPLKLPTEWVSELEYSIVLAYTGTSRRSSEIIRAQIL
                     NFEEGRTEAVAAMDRTREMAESMRRTLLAGDIPSMGALLDEAWRAKQRMSDSISSPTL
                     DALYAAALEAGALGGKISGAGG"
     gene            complement(978..1712)
                     /locus_tag="AC16YU_04770"
     CDS             complement(978..1712)
                     /locus_tag="AC16YU_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6064569.1"
                     /translation="MAAASSPDPPRSLPLPAVVLCGGLGTRLRAAVPGMPKVLAPVGG
                     RPFLDHLLRLLGSQGIREVILAAGVGGDQVMDAAGDGSAWGMDVTTVVEPRPLGTAGA
                     VAFAVERASIRAPFFVLNGDTYFTGELARMTRAAAVASVALVRVPDASRYGTVEHDGR
                     TGRVTGFREKDPNSRAPAWISAGAYLLSEAALGYLSAATSGSIERDVFPLLVGHGLRA
                     VRYPAATFIDIGTPADYERAATILAV"
     gene            complement(1742..2881)
                     /locus_tag="AC16YU_04775"
     CDS             complement(1742..2881)
                     /locus_tag="AC16YU_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6064570.1"
                     /translation="MSDSTNRHIAFVGPMHPYRGGIAQFLEAMVRGFDSRGCRVTAVT
                     FTRQYPGLLFPGKSQLEQSSGSPDIRPLRTLDSIGPVSWWRTAREIARARPDLVVLKY
                     WMPFLAPAFGSVVRLLRRQGIPSVIVVDNAIPHERRPGDLLLGRYLLRAAKGLVVMSE
                     AVEHDLRRLGITVPTRRVGHPVYDIFGPPQGSADARRALGLPEGAKVLLFFGFVRPYK
                     GLQVLLEAMRRVADELPDAHLVVAGEFYEPEAPYRDQIRKAGLTDRVHVFPDFIPSET
                     VSRYFSAADLVVQPYLSATQSGVAQIAFHFEKPVLATDVGGLAETLGDGEAGLIVRPG
                     DPGAVAAEVIRFFREDLAGRLVEGVRRRKTYYSWDRLLDAVESLA"
     gene            complement(2878..3849)
                     /locus_tag="AC16YU_04780"
     CDS             complement(2878..3849)
                     /locus_tag="AC16YU_04780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6064571.1"
                     /translation="MPDLSIVVPVLDEEESLPRLAEGIRDACEGAGLSFEVWMVDDGS
                     RDGSWSVIEAIHREDPRFAGLRFRRNYGKSAALAAAFERTVGRYVITMDADLQDDPDE
                     IPRLVARLEEGYDLVSGWKKKRRDPVSKTIPSRFFNMVTRRLSGIPLHDFNCGLKAYR
                     GEVVKSVRVYGELHRYIPLLAKWEGYGRITELPVVHHPRRFGRTKFGLERLLRGFLDL
                     LTVLFLTRFVVRPMHFFGTLGSLAFLVGLVISIWLSVEKIVFGLPIGDRPLLLMGVLM
                     ILVGVQMFTTGLIGEMVVGPRMMDTSRYWVDAEHAPAPGGRERADTE"
     gene            complement(3887..5833)
                     /gene="gyrB"
                     /locus_tag="AC16YU_04785"
     CDS             complement(3887..5833)
                     /gene="gyrB"
                     /locus_tag="AC16YU_04785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR6064572.1"
                     /translation="MTTPDDRSRGYGASNIQVLEGLTAVRKRPAMYIGDVGFRGLHHL
                     VYEVVDNSIDEALAGYCDRVEVSINADGSVTVTDNGRGIPVEEHPREKRSALEVVMTT
                     LHAGGKFDKDTYKVSGGLHGVGVSCVNALSRRLEAEVTRDGHLWRQAYEIGKPMGPVE
                     KIRKMKPGERTGTRVTFWPDATIFQVTEFRFDTLSERLRELAYLNRQIHITIEDLRDP
                     DDRLYEDYHFDGGIVEFVRYLDEARTPIVEDVIYIDDTDSEVPVELAMRYNNGYTENV
                     LSFVNNINTHEGGTHVAGFRAALTKTLKSYAERNNLLRNMKVELSGDDFREGITAVLS
                     VKVAEPQFEGQTKTKLGNSEVQGAVQTLVADRLQMWLEDHPKESRAIVDKVILAATAR
                     DAARKARELVQRKNAFSSSGLPGKLADCASRNPEECELYLVEGDSAGGSAKQARDRHF
                     QAILPLRGKILNVEKARLDKILANEEIKNLVTALGTGLATTSSEFDLERLRYHKLILM
                     TDADVDGAHIRTLLLTLLYRQLRPLIELGHVYVALPPLYKVKRGKDERYAWTEDDLHA
                     AVAEMGEAGRQKLSIQRYKGLGEMNPEQLWETTMNPESRMLQRVTIEDAAASDRIFST
                     LMGDSVEPRRKFIERNAKYATIDV"
     gene            complement(5925..6587)
                     /locus_tag="AC16YU_04790"
     CDS             complement(5925..6587)
                     /locus_tag="AC16YU_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6064573.1"
                     /translation="MTVASLVSKATPPLKPTDSVEYALGLLMEVRVRHLPVVDADRLL
                     VGVVSEEQLIDAGGPDVPLSALMGPVPVSVTTQTHLFEVARLMMENDLTAVPVLDNGR
                     YVGLVRRHELFDWFARTLATHAPGAILALEVAPRDFVLSQLVYAIEQSDVKVLSISSE
                     MPEGPEGRVRLTVKLNTRDASRVRLLLEHRGYHVMAAFGEDESDEDLLNRVQEFMRYL
                     EV"
     gene            complement(6743..8116)
                     /gene="hflX"
                     /locus_tag="AC16YU_04795"
     CDS             complement(6743..8116)
                     /gene="hflX"
                     /locus_tag="AC16YU_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR6064574.1"
                     /translation="MGDARAPGRGPDNELNGAEGAEESAILVGVQKGGEARWEVEDSL
                     SELALLADTAGAKVTDRVVQQVDRPNAATYLGSGKVTELAALAEERDTDLVVFDDDLS
                     PNQIRNLERAIGRKLIDRSALILDIFARHARSATAKTQVELAQLEYLRTRLTRQWTHL
                     SRQKGGIGTKGPGETQIETDRRLIGHRIATLRERLDAIDRQRKTQRRRREDYTRVSLV
                     GYTNAGKSTLMNALAHSNVRAEDRLFATLDATTRLVQLASNKQILLSDTVGFIRKLPH
                     RLIESFKSTLDEVREADALVHVVDTTHPRFEDQMTVVSETLAELGAVDKPTLLVFNKL
                     DAVSAPEQIHRLKSEFTEAAFVSALRGIGLGSLRSKLLACVEAHFIETVVCIPVTEAR
                     AIAHIRRVADVISEEYLELEDEALGPHPVARLHIRVSEKHYADLRPMLRSGRSTEFPA
                     VRGDLQE"
     gene            complement(8116..8676)
                     /locus_tag="AC16YU_04800"
     CDS             complement(8116..8676)
                     /locus_tag="AC16YU_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064575.1"
                     /translation="MLETVHSVLVIGFVAVTGSLLALTLVHRMRVRSVVLTWRSPRTA
                     TRPVWPILFIGLVLILYVFAANAVPDVPGTIFGGYVAGGVLWLMSSLASGAVMVTDFG
                     VVREFGKPGDAVAWMQVEDYFEATDDSRTIFVFIYEAENGQHRRLEVPVPAGHVDRFR
                     RIVRAHVDDRIETPVTHTVGRKALEG"
     gene            complement(8721..10829)
                     /locus_tag="AC16YU_04805"
     CDS             complement(8721..10829)
                     /locus_tag="AC16YU_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR6064576.1"
                     /translation="MTFLNPLLLLGLVAAAIPLIIHLFNFRRPKRIEFSSLAFLREVQ
                     RSTMQRVRIKQWLLLLLRTLAIAALVLSFARPILEGGLAGRLGGRSPASVAIVVDDTP
                     SMGQRDAGGAYFDQALDAARAVASATDARDEVFLVRVGGSSGQAAIGMPGGQTATERI
                     GQLGPSPFGGTLVGAIDRAAALLRAASQPVTEMYVISDLQASMLADSTDRPAAAPGQV
                     RIVPVGSRTDANVAVVDVRITSQIVEPGQPVRMEAVLVNHGTGTASAYVASVVLDGER
                     SAQASADLPPGIPVVVPFVVTPRGRGWLPGRVETEDDDFPLDNVRHFALYVPEARRLL
                     VVDGSAGTAGYLRRVFSERLTGERLRFEIVAVPESRLPAEDLSAYDVVLLGGPVDFTD
                     GEVDRIARFVSGGGGLFWFAGDGAVLEDYNLLLAALGAGRVEAVTSGTLADPIDSVER
                     TDLAHPLFQGVLEGGGRSPERPTLYRRMTYRPGTGTENTVMELAGGTPFLQEIRSGRG
                     SVLFMASAVDPAWTDLPTRGLFVPLMYRSVFLLSSAGAVSGESYVLGRGGELLLPGPV
                     AEQIQVRTPGGELVTPARRDGAGGTLLRLDEVLTEPGAYAVEVGGRVIRRVVANPDPR
                     ESDLARMEPPDAVDALGSRFGIDADVLRIESGDPAAARAAVTAQRQGVELWNVFMGLA
                     LLFLVLEMIVARHWRPESVS"
     gene            10949..13798
                     /gene="uvrA"
                     /locus_tag="AC16YU_04810"
     CDS             10949..13798
                     /gene="uvrA"
                     /locus_tag="AC16YU_04810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6064577.1"
                     /translation="MSTSPEATRATEAPRRNGAERSIVIRGARQHNLKGIDLDLPRGR
                     LVVITGPSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLERMDKPDVDLITGLAPAI
                     AIEQKTGTRNPRSTVATQTELYDHLRLLYARVGTTISPVSGEPVTRDSPRSAAEHVSA
                     TLPDGTRFHVCFPVPEHGRSSIGKELAVLLKRGFFRLVVLPGAKGSEKVVDLNETDPE
                     SVRVSRDRLLVLVDRLAVRTGSDEATSRLADSVEQAFREGGGRCVIQVADGGPRLDFS
                     EFFERDGIRFEEPTPHLFSFNSPLGACPSCQGFGRITGIDPDLVVPNPDLSIRQGAIA
                     PFRSEQWSAYFRELIRLAASEKIDIDAPWHRLTERDLDLVWNGKGEYDGIRGLFRKLE
                     RQNYKMHYRIFHARFRGYTRCPECSGNRLRPEALHVRVGGQHIGQVCELTVGDARKFF
                     DELRLTGHQEQIAGRVLAEIRKRLRFLDEVGLDYLTLDRLSQTLSGGESQRIHLATSL
                     GSSLVGSLYVLDEPSIGLHPRDTDRLIRILEHLRDIGNTVLVVEHDAEMMRRADYIVD
                     LGPGSGVHGGEVVAKGTPVDVLASPDSLTGAYLSGRRSVPVPPVRRSGHPDDVIRIEN
                     ARQHNLKRIDVRIPLGMIVCVTGVSGSGKSTLLHDTLYRGIARLKGMELGDGKVGVHD
                     AIRGHQLVDAVELVDQSPIGRSPRSNPVTYVKAFDHIRQLFADTYQARIRGCKPGYFS
                     FNVPGGRCETCQGEGQVKIEMQFLADLYLECEACKGTRFKQEAREIHYRGRSIDEVLS
                     MTVEEALEFFADEAALARKLQVLVDIGLGYITLGQPSNTLSGGEAQRIKLAAHLGKAG
                     RDRILYLFDEPTTGLHTDDIRKLLDAFRALVDDGHSVVLVEHNLDVIKSADHVIDLGP
                     EGGIGGGTVIAAGTPEDVAGVGASYTGKFLRDLLG"
BASE COUNT         2230 a         4880 c         4648 g         2118 t
ORIGIN      
        1 cgccgcccgc accggagatc ttgcccccca gggcgccggc ttcgagtgcc gcggcgtaca
       61 gcgcgtccag ggtcggactc gatatggaat cggacatccg ctgcttcgcc cgccaggcct
      121 cgtcgaggag ggcgcccatc gaggggatgt cacccgcgag aagcgtccgg cgcatggact
      181 ccgccatctc ccgtgtgcgg tccatggcgg cgacggcttc ggtccttcct tcctcgaaat
      241 tgaggatctg cgcccggatg atttcggagg accgccggct ggtcccggtg tacgcgagca
      301 cgatggagta ctcgagctcg ctcacccatt ccgtcgggag cttgagcgga gtcacgagtg
      361 ccgggccggg accgaattcc aggtagttga atccaccgaa ggctgctgcg tactggtcct
      421 gccgaccacc cgcgatgccc aggtcgatcc gttcgatctc gtaggccagg tccgcggtct
      481 cgtaccggga gaggccgagc ccgagccagg cgtcgaatgc gcccacgagc gccacgacca
      541 tcgtggatga cgcacccagc ccggagcctg gaggacaatc cgcgtgcgtg aagaggtcaa
      601 agcccggaag ccgacgaccc tcggtcatgc gacgcacgac tcccttggcc aggctcatct
      661 ggtagtccac gtccggcacc ggtgcgtcgg tccggaacgc cacttcctcg cccaggtcca
      721 gcgattgcat ccggatctgg tccgacccgt tcggcgccag cgtgacgtag gcgtaacggt
      781 tgatggcggc attcagcacg cggccgcccc gctcctcggc gtacggcgac acgtccgtgc
      841 ctccgccggc gaaactgatg cgcagcggag ccctcgatcg gatgactctg ctcacgatgg
      901 ccctgtgcga gatcggtgtt gaacggtcgg gtaccgccac gaagtcctca acaaaccgcc
      961 tgcgcgcgcg gggttcctca gactgccaga atcgtcgcgg cccgctcgta atcggccggc
     1021 gtcccgatat cgatgaacgt ggcggccgga tagcgcacgg ccctgaggcc gtgtcccacg
     1081 aggagcggaa acacgtccct ctcgatcgat ccggacgtcg ccgccgacag gtacccgagt
     1141 gcggcctccg acagcaggta ggccccggca ctgatccacg cgggggcgcg cgagttcggg
     1201 tccttctcac ggaagcccgt gacccggccg gtccggccgt catgttcgac cgtaccgtag
     1261 cgggatgcgt ccggaacgcg caccaatgcg acagatgcca ccgcggcggc ccgcgtcatc
     1321 cgcgcaagct ctcccgtgaa gtacgtatcg ccgttgagca cgaagaacgg agcccggatc
     1381 gacgcccgtt ccacggcgaa cgccacggcc cccgccgtcc ccaggggacg cggctctacg
     1441 accgtcgtga catccatccc ccacgccgat ccatcgcccg ccgcgtccat cacctggtcc
     1501 cctccgacgc ccgccgccag gatcacctcg cggatgccct ggctcccgag cagtcgaaga
     1561 aggtggtcca ggaacggtct tcccccgacc ggtgccagca ccttgggcat tcccggaaca
     1621 gcggcgcgga gcctggtccc caggcccccg cacaggacga ccgccggaag cgggagcgag
     1681 cggggcggat cgggagatga tgctgcagcc atcgggtact ggcaccgggc gcatccacgg
     1741 gtcaggccag ggactccacc gcgtcgagga gcctgtccca cgaataatac gtctttcgcc
     1801 tccgcacgcc ctcgaccagg cggccggcga ggtcctcccg gaagaagcgg atcacttcgg
     1861 cagccaccgc gccgggatct cccgggcgca cgatcagccc ggcttcgccg tcgcccagtg
     1921 tctcggccag gccacccaca tcggttgcga ggaccggctt ctcgaaatga aacgcgatct
     1981 gtgccacccc gctctgcgtc gcggacaggt acggctgtac caccaggtcc gccgcggaga
     2041 agtacctcga aacggtctcc gagggtatga aatcgggaaa tacgtgaacg cgatccgtga
     2101 gacccgcctt ccggatctgg tcgcggtacg gagcctcggg ctcgtagaat tcgccggcca
     2161 caaccaggtg agcgtcggga agttcgtccg cgacgcggcg catggcctcg agaaggacct
     2221 gcagcccctt gtagggccga acgaagccga agaacagcag gacctttgcc ccttccggca
     2281 gaccgagcgc cctccgggcg tcggcgctcc cctgcggcgg cccgaaaatg tcgtagacgg
     2341 gatggcccac ccggcgcgtc ggaacggtga tcccgagacg gcggaggtcg tgctccacgg
     2401 cttccgacat caccacgagg cctttcgccg cacggagcag atatcggccc agcagcaggt
     2461 ctcccggcct ccgctcatgc ggaatcgcgt tgtcgacgac gatgacggac ggtatgccct
     2521 gccgcctcag gagccggacc acgctcccga aggccggggc aaggaacggc atccagtact
     2581 tgagcaccac gagatcgggc cgggcccgtg cgatctcgcg cgcggtccgc caccacgaca
     2641 ccggtccgat gctgtcaagc gtccggagtg gccggatatc cgggctgccg gaggactgct
     2701 cgagctgcga tttccccggg aagagcaggc ccggatactg ccgggtgaac gtgactgcgg
     2761 tcacccgaca gcccctcgaa tcgaaaccgc gtaccatcgc ctccaggaac tgggcgatgc
     2821 cgccccgata cggatgcatc ggccccacga acgcgatgtg ccggttcgtt gaatcgctca
     2881 ttccgtgtcc gcacgctccc ggccgccggg cgccggtgca tgctccgcgt ccacccagta
     2941 gcgggacgtg tccatcatgc gcggcccgac caccatctcg ccgatgagcc ccgtcgtgaa
     3001 catctggacg ccgaccagga tcatcagcac gcccatgagc agcagcggcc gatccccgat
     3061 cggcaggccg aagacgatct tctcgaccga gagccagatg ctgatcacga gtccgaccag
     3121 gaacgccagg ctgccgagcg tgccgaagaa gtgcatcggc cggaccacga accgggtcag
     3181 gaagagcacg gtgagcaggt ccaggaatcc gcgaagcagc cgctcgagtc cgaacttggt
     3241 ccggccgaac cggcggggat ggtgcacgac gggcagttcg gtgatgcggc cgtagccctc
     3301 ccacttggcc aggagcggga tgtagcgatg gagctcgccg tagacccgca cgctcttgac
     3361 cacctcgccc cggtaggcct tgagcccgca gttgaaatcg tggagcggga tcccggagag
     3421 cctgcgcgtg accatgttga agaagcggct cggaatggtc ttcgacacgg ggtcgcgccg
     3481 cttcttcttc caccccgaca ccaggtcgta cccttcctcg agccgggcca cgagccgcgg
     3541 gatctcgtcc ggatcgtcct gcaggtcggc gtccatcgtg atcacgtagc gcccgacggt
     3601 ccgctcgaac gccgccgcga gcgcggcgga cttcccgtag ttgcggcgga accggaggcc
     3661 ggcgaaccgc ggatcttccc tgtggatggc ctcgatcacg gaccaggagc cgtcgcggga
     3721 accgtcgtcc accatccaca cctcgaacga gaggcccgcc ccctcgcagg cgtcccggat
     3781 tccctcggcg aggcgcggaa gggactcctc ctcgtcaagg acggggacta cgatgctcag
     3841 atcgggcatg gagcgcatgg gctgccggag cgggatcggc ggacgctcag acgtcgatgg
     3901 tcgcgtactt ggcgttccgt tcgatgaact tgcgccgggg ctcgaccgaa tctcccatca
     3961 gcgtgctgaa gatgcggtcg gaggcggcgg cgtcctcgat cgtcacgcgc tgaagcatac
     4021 gcgattccgg gttcatcgtc gtctcccaca gctgctcggg gttcatttcg cccaggccct
     4081 tgtaccgctg gatcgagagc ttctggcgcc ccgcctcgcc catctcggcg acggcggcat
     4141 gcaggtcgtc ctccgtccag gcgtaccgct cgtccttgcc ccgcttcacc ttgtagagcg
     4201 gcggcagggc cacgtacacg tgcccgagct cgatgagcgg ccggagctgc cggtagagca
     4261 gggtcaggag gagcgtccgg atgtgcgcgc cgtccacgtc ggcgtccgtc atgaggatca
     4321 gcttgtggta gcgcaggcgc tccaggtcga attcgctgga ggtcgtcgcc aggcccgtgc
     4381 ccagcgccgt gaccaggttc ttgatctcct cgttggccag gatcttgtcg agccgggcct
     4441 tctcgacgtt gaggatcttg ccgcgcagcg gcaggatcgc ctggaagtgc cggtcgcgcg
     4501 cctgcttcgc cgatccgccg gccgagtcgc cctcgaccag gtacagctcg cattcctccg
     4561 gattgcggga ggcgcagtcg gcgagcttgc cgggcagtcc ggacgacgag aaggcgttct
     4621 tgcgctggac gagctcgcgg gccttgcgcg ccgcatcccg ggccgtcgcc gcgagaatca
     4681 ccttgtccac gatcgctcgg gactccttcg ggtgatcctc gagccacatc tgcaggcgat
     4741 ccgcgaccag cgtctggacg gcgccctgga cctccgagtt gccgagcttg gtcttggtct
     4801 gcccttcgaa ctgaggctcg gccaccttga ccgagaggac ggccgtgatg ccctcgcgga
     4861 agtcgtcgcc ggagagctcc accttcatgt tccggagcag gttgttgcgc tccgcatagc
     4921 tcttgagcgt cttggtcagc gccgcccgga atccggcgac gtgcgtgccg ccctcgtgcg
     4981 tgttgatgtt gttcacgaac gagagcacgt tctcggtgta cccgttgttg tagcgcatgg
     5041 ccagctccac cgggacctcc gagtcggtgt cgtctatgta gatgacgtcc tcgacgatcg
     5101 gcgtacgggc ctcgtccagg taccggacga actcgacgat gcccccgtcg aagtggtagt
     5161 cctcgtagag ccggtcgtcg gggtcccgca ggtcctcgat ggtgatgtgg atctggcgat
     5221 tcaggtaggc cagctctcgc agccgctccg acagcgtgtc gaagcggaac tccgtgacct
     5281 ggaagatcgt ggcgtcgggc cagaaggtga cgcgggtccc ggtgcgctcg cccggcttca
     5341 ttttgcggat cttctcgacc gggcccatcg gcttgccgat ctcgtacgcc tgccgccaca
     5401 ggtgaccgtc gcgcgtcacc tcggcttcga gccgcctcga gagcgcgttc acgcacgaca
     5461 cgcccacgcc gtgcagtccg ccggagacct tgtacgtgtc cttgtcgaac ttgccgcccg
     5521 cgtgcagcgt ggtcatcacg acctcgagag cggaccgctt ctcccgcgga tgctcctcga
     5581 ccgggatgcc ccgcccgttg tccgtcaccg tcaccgatcc gtcggcattg atcgagacct
     5641 cgacccggtc acagtaaccc gcgagcgcct cgtcgatcga gttgtcgacc acctcgtaga
     5701 ccaggtggtg caggccgcgg aagcccacgt cgccgatgta catggcaggc cgcttgcgga
     5761 ccgccgtgag gccttcgagg acctgtatgt tggacgcgcc gtagcccctg gatcgatcgt
     5821 cgggagtcgt catgtgccgt gtcggaaacg cggaatagcg gaaaatgccc aatccaatct
     5881 tgtacgatcg ggaggggccg cagttcttcc atccgacgag gccgtcagac ctcgaggtag
     5941 cgcatgaact cctgcacccg gttcagcagg tcctcgtcgc tctcgtcttc gccgaaggcg
     6001 gccatgacgt gatagccgcg atgctcgagg agcaggcgca cgcgagacgc gtcccgggtg
     6061 ttcagcttca ccgtgagacg cacgcggccc tccgggcctt ccggcatctc gctcgagatc
     6121 gaaagcacct tgacgtcgct ctgctcgatc gcgtacacga gctgcgacag gacgaagtct
     6181 ctcggcgcca cttccagcgc gaggatggcg cccggtgcgt gggtggccag cgtacgggcg
     6241 aaccagtcga agagctcgtg cctgcgcacc aggcccacgt agcgtccgtt gtccaggacg
     6301 ggaacggccg tgagatcgtt ctccatcatg aggcgggcca cctcgaacag gtgcgtctgc
     6361 gtcgtgaccg agaccggcac cggtcccatc agcgcggaga gcggcacgtc cggcccgccg
     6421 gcgtcgatca gctgctcttc cgacaccacg cccacgagca ggcggtcggc gtcgacgacc
     6481 ggaaggtgcc gcacccggac ctccatgagc aggccgagcg cgtactccac cgagtcggtc
     6541 ggcttgagcg gcggcgtcgc cttgcttacg agactggcta cggtcatggc cggagggtgt
     6601 gtagcgggaa gctgtcgatg cttacatgat gcaatcgccg tgccgtttcc gttccgatgt
     6661 gtggtccccg caaattccgc gtgcgctgtt gcccgggaaa tacgaaatga gcgcggcgcc
     6721 gcgcaacgca gtgccgggta ccctactcct ggagatcccc gcgcacggca ggaaattccg
     6781 tgctccggcc gctgcgcagc atcgggcgca ggtccgcgta gtgcttctcc gagacccgga
     6841 tgtgaaggcg ggcgaccgga tgcgggccca gcgcctcgtc ctcgagctcg agatattctt
     6901 ccgagatcac gtccgcgaca cgccggatgt gggcgatggc gcgcgcctcg gtcaccggga
     6961 tgcacacgac cgtctcgatg aaatgcgcct cgacgcaggc gagcagcttg ctgcgcagcg
     7021 agcccagccc gatgccgcgg agcgccgaaa cgaaggccgc ctcggtgaac tcggacttca
     7081 ggcggtggat ctgctcgggc gcgctcacgg cgtccagctt gttgaacacc aggagcgtcg
     7141 gtttgtccac cgcgccgagt tcggcgaggg tctcggacac caccgtcatc tggtcctcga
     7201 accgcgggtg cgtcgtgtcc acgacgtgga ccagggcatc ggcctcgcgc acctcgtcga
     7261 gcgtcgactt gaagctctcg atcaggcggt gcggaagctt gcggatgaag ccgaccgtgt
     7321 ccgagagcag aatctgcttg ttggatgcga gctgaacgag ccgcgtggtg gcgtcgagcg
     7381 tcgcgaacag ccggtcctcg gcgcgcacgt tcgagtgggc gagggcgttc atcagcgtgg
     7441 acttgcccgc gttcgtgtac ccgaccaggg acacgcgcgt gtagtcctcg cgacggcggc
     7501 gctgggtctt gcgctgccga tcgatcgcgt cgagccgctc gcgcagggtc gcgatccggt
     7561 ggccgatgag ccgccggtcc gtctcgatct gcgtctcgcc cggtcctttc gttccgatgc
     7621 cgcccttctg gcgggacagg tgcgtccact ggcgggtcag gcgggtccgc aggtattcca
     7681 gctgggccag ctccacctgc gtcttggccg tggcggaacg ggcgtgccgc gcgaagatgt
     7741 ccaggatgag cgccgaacgg tcgatcagct tccggccgat ggcccgctcc aggttgcgga
     7801 tctggttcgg ggacaggtcg tcgtcgaaca cgacgagatc cgtgtcacgc tcctccgcca
     7861 gtgcggcgag ctccgtgacc ttgccgctgc ccaggtaggt cgccgcgttc gggcggtcca
     7921 cctgctggac cacccggtcc gtgaccttcg ccccggccgt atcggccaga agggccagct
     7981 cgctgagcga gtcctccact tcccaccgcg cctcaccgcc cttctgcacc ccgaccagga
     8041 tcgcggactc ctccgcgccc tccgccccgt tcagctcgtt gtccggcccg cgtccgggcg
     8101 ccctggcatc ccccatcaac cttcaagagc cttgcggccc acggtgtgtg tcacgggcgt
     8161 ctcgatccgg tcgtccacgt gcgcccggac gatgcggcgg aagcggtcca cgtgcccggc
     8221 cggaaccgga acctccaggc gacggtgctg cccgttctcc gcctcgtaga tgaacacgaa
     8281 tatcgtcctc gaatcgtccg tcgcctcgaa gtagtcctcg acctgcatcc acgcgaccgc
     8341 gtcgccgggc ttgccgaact cgcggaccac tccgaagtcc gtcaccatca ccgcgcccga
     8401 cgcgagcgac gacatcagcc agagcacgcc gcccgccacg tacccgccga aaatggtgcc
     8461 ggggacgtcg ggcacggcat tcgccgcgaa cacgtacagg atcaggacca gcccgatgaa
     8521 gaggatcggc cacaccggcc gggtggccgt gcggggcgag cgccaggtca gcacgacgct
     8581 gcgcacgcgc atccgatgca cgagcgtgag cgcgagcagc gagcccgtca cggcgacgaa
     8641 gccgatgacc agtaccgagt ggacggtttc cagcacgctg ggtggggtgg ctggttatgg
     8701 gcgtcccgat ccgtcgacgg tcaggagacc gactcgggcc tccagtgacg cgcaacgatc
     8761 atttccagga ccagaaacag caacgcaagc cccatgaaga cgttccagag ctcgacgccc
     8821 tggcgctgcg cggtcaccgc cgcgcgggcg gcggcggggt ccccggactc gatccgcagc
     8881 acgtcggcat cgattccgaa acggctcccg agcgcgtcca cggcatccgg gggctccatc
     8941 cgcgccaggt ccgactcccg cggatccgga ttcgcgacca ccctgcggat gacccgcccg
     9001 ccgacctcta ccgcgtacgc cccgggctcg gtgagcacct cgtcgagccg aaggagcgtc
     9061 cccccggccc cgtcgcgccg ggccggcgtg accagctccc cgcccggggt ccggacctgg
     9121 atctgctccg cgaccgggcc cggaagcagg agttccccgc cgcggccgag cacgtacgac
     9181 tcgccggaca cggcgcccgc cgacgacaga aggaacaccg agcggtacat cagcgggacg
     9241 aacagccccc gcgtcggaag gtcggtccag gccgggtcca cggccgacgc catgaacagc
     9301 accgagccgc ggccgctgcg gatctcctgg aggaaaggcg tgccgccggc cagctccatg
     9361 acggtgttct cggtcccggt ccccggccgg taggtcatgc gccggtacag cgtggggcgt
     9421 tcgggcgagc ggccgccacc ctccaggacg ccctggaaca gggggtgggc gaggtccgtg
     9481 cgttcgacgg aatcgatcgg atcggccagc gtcccggacg tcacggcctc cacccggccc
     9541 gcgcccagtg ccgcgagcag caggttgtag tcctccagta cggcgccgtc gcccgcgaac
     9601 cagaacagcc ccccgccgcc cgagacgaat cgcgcgatcc ggtccacctc gccgtccgtg
     9661 aaatcgaccg gtccgccgag gaggacgacg tcgtaggccg acaggtcctc ggccggaagc
     9721 cggctctcgg ggacggcgac gatctcgaac cgcagcctct cgcccgtgag ccgctccgag
     9781 aagacacggc gcaggtagcc ggccgtcccg gccgagccgt cgaccacgag caggcgcctg
     9841 gcctcgggca cgtacagggc gaaatgacgg acgttgtcga gcgggaagtc atcgtcctcc
     9901 gtctcgacgc ggccagggag ccaccccctg ccgcggggcg tcacgacgaa gggcacgacg
     9961 acggggatcc cgggcggcag gtccgcgctg gcctgcgccg agcgctcccc gtcgaggacg
    10021 accgaggcga cgtacgcgga cgccgtgccc gtcccgtggt tcacgaggac cgcctccatg
    10081 cggaccggct ggccgggctc gacgatctgc gaggtgatcc gcacgtcgac gaccgccacg
    10141 ttggcgtcgg tccgcgagcc gacggggacg atccggacct gccccggggc cgctgccggc
    10201 ctgtccgtcg agtctgcgag catggaggcc tggaggtccg agatcacgta catctcggtc
    10261 acgggctgcg acgccgcgcg gagcagggcc gccgcgcgat cgatggctcc gaccagcgtc
    10321 ccgccgaacg gggacggccc gagctgaccg atcctctccg tcgcggtctg cccgcccggc
    10381 atcccgatgg ccgcctgccc gctactcccg cccacgcgca cgaggaacac ctcgtcacgg
    10441 gcgtccgtcg ccgaggccac ggcgcgggcc gcgtcgagcg cctggtcgaa gtacgcccct
    10501 ccggcgtccc gctggcccat ggacggcgtg tcgtcgacca cgatcgccac gctggcgggc
    10561 gagcggccgc cgaggcggcc ggcgagccct ccctccagga tcgggcgggc aaacgacagc
    10621 accagggccg cgatcgccag cgtccggaga aggagcagca gccactgctt gatgcggacg
    10681 cgctgcatcg tggaccgctg gacctccctc aggaacgcca gggacgagaa ctcgattcgt
    10741 ttgggcctgc ggaaattgaa caggtggatg atcagcggga tggccgccgc caccagtccg
    10801 aggagcagga gcggattgag aaaggtcatc gtccgggccg ggtcggcggc ttcgaacgtg
    10861 acccttcggc ggccagttcc gtcgatcgga accggcatcc ttgtgcggat ccgggcgtac
    10921 aacgcggctc tccgatcccc acacgaccgt gtcgacgtcc cccgaagcca cccgagcgac
    10981 cgaggccccc cgcaggaacg gggcggaacg gtccatcgtc atccgggggg cgcgccagca
    11041 caacctgaag gggatcgacc tggacctgcc gcggggccgg ctcgtggtga tcaccggccc
    11101 gtccggctcg gggaagtcgt ccctcgcctt cgacacgatc tacgccgagg gacagcggcg
    11161 gtacgtggaa agcctctccg cgtacgctcg ccagttcctg gagcggatgg acaagcccga
    11221 cgtggacctg atcacggggc tggcgcccgc catcgccatc gagcagaaga cgggcacgcg
    11281 gaatccgcgg tccaccgtcg cgacgcagac cgagctctac gaccacctcc gcctgctcta
    11341 cgcccgcgtc gggacgacca tctcaccggt gagcggcgag cccgtcacgc gcgattcacc
    11401 ccgctcggcg gcggagcacg tctcggcgac cctcccggac gggacgcgct tccatgtctg
    11461 cttcccggtc ccggagcacg gccgcagctc catcggcaag gagctcgcgg tgctcctcaa
    11521 gcggggtttc ttccgcctgg tggtgcttcc gggcgcgaag ggatccgaga aggtggtcga
    11581 cctgaacgaa accgatccgg agagcgtgcg cgtgagccgc gaccgcctcc tggtcctggt
    11641 agaccggtta gccgtccgca ccggctcgga cgaggccacc tcgcgcctgg cggactccgt
    11701 ggaacaggcc ttccgcgagg gaggcggccg ctgcgtgatc caggtggcgg acggcgggcc
    11761 gaggctggac ttcagcgagt tcttcgagcg cgacggaatc cggttcgagg agcccacgcc
    11821 ccacctcttc tccttcaact ccccgctcgg cgcctgccct tcgtgccagg gattcggccg
    11881 gatcacgggc atcgatcccg acctggtcgt gccgaatccc gacctctcga tccggcaggg
    11941 cgcgatcgcc cctttccggt ccgagcagtg gagcgcgtac ttccgcgagc tgatccgcct
    12001 ggcagcctcc gagaagatcg acatcgacgc gccctggcac cggctcacgg agcgggatct
    12061 cgacctcgtg tggaacggca agggtgagta cgacgggatc cgcgggctct tccggaagct
    12121 ggagcggcag aactacaaga tgcactaccg catcttccac gcccgcttcc gggggtacac
    12181 gcgctgcccg gaatgctccg gcaaccggct gcggcccgag gcgctccacg tccgggtcgg
    12241 cgggcagcac atcggccagg tgtgtgagct gacggtgggc gacgcgcgga agttcttcga
    12301 cgagctccgc ctcaccgggc accaggagca gatcgccggt cgcgtcctgg cggagatccg
    12361 caagcgcctg cgcttcctgg acgaggtggg gctcgactac ctgaccctgg accggctgag
    12421 ccagacgctc tccggcggcg agagccagcg gatacacctc gcgacgtcac tcggctcgtc
    12481 gctcgtcggc tcgctgtacg tcctggacga gccgtccatc gggctgcacc cgcgcgacac
    12541 cgaccggctg atccgcatcc tggagcacct gcgggacatc ggcaacacgg ttctcgtcgt
    12601 cgagcacgac gcggagatga tgcggcgggc cgattacatc gtcgacctgg gtcccggatc
    12661 cggcgtgcac ggcggggagg tcgtagcgaa ggggacgccg gttgacgtgc tcgcgagccc
    12721 ggactccctg acgggcgcct acctgtcggg gcgccggtcg gtgcccgttc cgcccgtgcg
    12781 ccggtcgggg cacccggacg acgtgatccg gatcgagaat gcccgccagc acaacctgaa
    12841 gcggatcgac gtccggatcc ccctgggcat gatcgtctgc gtgacgggag tgagcgggtc
    12901 cggcaagtcc acgctccttc acgacacgct gtaccgggga atcgcgcgcc tgaagggaat
    12961 ggagctcggt gacgggaagg tgggcgtcca cgacgccatc cggggccacc agctcgtgga
    13021 cgccgtcgag ctcgtggacc agtcgccgat cggccgcagc ccgcgatcga acccggtcac
    13081 ctacgtcaag gccttcgacc acatccggca gctcttcgcc gatacctacc aggcccgcat
    13141 ccgcggatgc aagccgggat atttctcgtt caacgtgccc ggcggccgtt gcgagacgtg
    13201 ccagggcgag gggcaggtga agatcgagat gcagttcctc gcggacctgt acctcgagtg
    13261 cgaggcgtgc aagggtaccc ggttcaagca ggaggcgcgg gagatccatt acaggggccg
    13321 aagcatcgac gaggtcctgt cgatgaccgt ggaggaagcg ctggagttct tcgccgacga
    13381 ggcggcgctg gcccgcaagc tgcaggtgct cgtcgacatc gggctgggct acatcacgct
    13441 cggccagccg tcgaacacgc tctccggcgg cgaggcccag cgcataaagc tggcggccca
    13501 cctgggcaag gccggacgcg accgcatcct gtacctgttc gacgagccca cgacggggct
    13561 gcacacggac gacatccgca agctgctcga cgccttccgg gcgctcgtcg acgacggcca
    13621 ttcggtcgtg ctggtcgagc acaacctgga cgtcatcaag tcggccgacc acgtgatcga
    13681 cctgggaccc gaaggcggca tcggcggcgg caccgtgatc gcggctggca cgcccgagga
    13741 cgtcgcaggc gtcggggcca gctacaccgg caagttcctg cgcgacctgc tgggctgacc
    13801 tgtccgcagg tgcttcgcga gcatcacgac cagcagcacg atccccgcca acgccagcaa
    13861 ggcgcccgtg cgaaac
//
LOCUS       DBNHQZ010000182         5356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1815267, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000182 DBNHQZ010000000
VERSION     DBNHQZ010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1815267"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="AC16YU_04815"
     CDS             complement(<1..1681)
                     /locus_tag="AC16YU_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HIR6064578.1"
                     /translation="MTLHAADPLGPSLSHVLLLVLGTLSILSAAACSAPPSDIVPSRL
                     FDDGMVLQRDVDLRVWGTARPDAMVTVDFLGASYQAPAGPDGTWMIRLDAAPAGGPHE
                     MRISGEGYERTIRDVLVGDVWVASGQSNMEWTVDSAWDAQAEIASADDPMIRQFLVPK
                     AWSDSPVDSVVGGPWTPADPGHVGGFTAVGYFFAREIRQHHDVPIGIIHASWGGSRLE
                     PWMSAESLGLDEAGVAALIQGEREREELALAAIRERIGPLPERDGGLVDGAALWADPD
                     LDEAGWERIPVPSTWEAAGFPGMDGIAWYRTAFDLTAAEAAAGATLGLGMIDDSDRTW
                     VNGSEVGGMEMSWNVAREYEVPASALREGRNVVAVRVVDTGGGGGIAGSPDQVFVEAG
                     GRRRPLTGEWAFRVGEVRVVMDASRNQVPVLLYNAMIHPLLWYRIKGAIWYQGESNTG
                     PGDALAYRDLFATMIQDWRSRWGVGDFPFLWAQLANFNPPADQPGESGWAVLRESQSE
                     ALSVSNTAQAVLIDIGEAADIHPRNKQDVGYRLALGARAIAYGEDLVHSGPT"
     gene            complement(1678..3183)
                     /locus_tag="AC16YU_04820"
     CDS             complement(1678..3183)
                     /locus_tag="AC16YU_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6064579.1"
                     /translation="MRADLVLPLVAALWMAVSPAAQARQELGTFEGTTDIGPVGVEGA
                     LAYDAVAQRYTVTGSGENMWFGSDAFRFVWRRVSGDFTIRARVAFRGDGGHQHRKIGV
                     IARSSLDPGSPYVDVAVHGDGMTSMQYRRGPGANTEEVRSSAGGPDVVQLERRGNRFV
                     MSVARFGEPFVTETLDGPALGDDLYVGLFVCSHDNAVAETADFDNVRLVVPPPAGWVP
                     YRDYIGSRLEVLEVDTGHRRVFHRSKGSIQAPNWTPDGKALIFNQDGLLYRFDLASRE
                     ATPIDTDFATRNNNDHVLSFDGSMLGISHHSEDHGGESIVYTVPASGGVPRAVTPVGP
                     SYLHGWSPDGRHLVYTAQRGGDYDIYRIPAGGGDEVRLTDAPGLDDGPEYAPDGRSIW
                     FNSVRSGTMQLWRMGADGSKPTRMTRDGYNDWFPHVSPDGSRVVFLSFMADVDPSDHP
                     FYRPVYLRMMPAGGGEPRIIAYVFGGQGTINVPSWSPDGRFVAFVSNSDLP"
     gene            complement(3173..3499)
                     /locus_tag="AC16YU_04825"
     CDS             complement(3173..3499)
                     /locus_tag="AC16YU_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HIR6064580.1"
                     /translation="MIIVNTDFVHGYSVRETLGMVRGNTIRAKHLGKDIMAGLRTLVG
                     GEIKEYTEMLTEARNEALQRMEAEARRLGADAVINVRFMTAQVMAGSAELLAYGTAVK
                     LQADAR"
     gene            complement(3541..3774)
                     /locus_tag="AC16YU_04830"
     CDS             complement(3541..3774)
                     /locus_tag="AC16YU_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064581.1"
                     /translation="MIRFTRILGYLLSGVGVLTLLVWMIRPLRDVWPFLLQLPWPIRL
                     GLGTLLLGFLLVMASLIAERVEERGEDRALRDR"
     gene            complement(3802..4731)
                     /locus_tag="AC16YU_04835"
     CDS             complement(3802..4731)
                     /locus_tag="AC16YU_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6064582.1"
                     /translation="MKWTRRDFIRASAGTGAALLAGVRPASSRTRKLILKPIASSGEE
                     VPVIGIGTREYRAGAGADELVPFVDTMEVFLEAGGRVIDTAPSYGNSEEVLGRVLARL
                     EARTRVFMSTKVDQEGRDAGIERMERSLGLLGGDSVDLMQVHNLRDVDTQLATLREWK
                     EAGRVRYVGITTSSSRQYEDFEATMKRHELDFVQVDYSLANRGAAERLLPLAADRGMA
                     SLINLPFGRGRLFARVGDRPVPEWAAEAGIASWGQFFLKYVVSHPAVTVAIPGTTKPH
                     HARDNMGAAMGELPDASMRRRMEEFYDALPEPG"
     gene            complement(4824..>5356)
                     /locus_tag="AC16YU_04840"
     CDS             complement(4824..>5356)
                     /locus_tag="AC16YU_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6064583.1"
                     /translation="FQQAFLVGEAIADRAARASFFAQIDLAVNILTIVLQVAVTARIL
                     RWLGVGFSLMIVPALTMIGFVGLGLVPLLVVLVVLQVLYRAGRYGIAKPVREVLFTVV
                     SREERYKSKAFLDAAVYRGGDLVSGWIYAGLAAVGLSVGAIALVAAPLAALWVLMSLN
                     LGRRHELLAAAEPGTA"
BASE COUNT          816 a         1932 c         1862 g          746 t
ORIGIN      
        1 acgtcggacc cgagtgcacg aggtcctccc cgtaggcgat ggcccgcgcc ccgagcgcga
       61 gccggtagcc cacgtcctgc ttgttgcgcg ggtggatgtc cgcggcctcg ccgatgtcga
      121 tgagcacggc ctgcgccgtg ttggagaccg agagcgcttc ggactgcgat tcgcgaagca
      181 cggcccatcc gctctcccct ggctggtccg ccggcgggtt gaagttggcg agctgcgccc
      241 acaggaacgg gaagtcgccc acgccccagc gggagcgcca gtcctggatc atcgtcgcga
      301 agaggtcccg gtacgcgagc gcgtcgcccg ggcccgtgtt cgactcgccc tggtaccaga
      361 tggcgccctt gatccggtac cagagcagcg ggtggatcat ggcgttgtac aggaggacgg
      421 gcacctggtt acgactcgcg tccatgacca cgcggacctc gcccacgcgg aacgcccact
      481 cgcccgtgag cggacgccgc cgcccgcccg cctccacgaa cacctggtcc ggcgagcccg
      541 cgatgccgcc tcctccgccc gtgtccacga cgcggacggc gacgacgttg cgtccctcgc
      601 gaagcgccga cgccggcacc tcgtattcgc gcgcgacgtt ccacgacatc tccatgccgc
      661 ccacctcgga gccgttcacc caggtccggt cggagtcgtc gatcattccc aggcccagcg
      721 tggctccggc cgccgcctcc gcggcggtca ggtcgaaggc cgtccggtac caggcgatgc
      781 cgtccatgcc cgggaagccg gccgcctccc aggtggacgg gacggggatg cgctcccacc
      841 ccgcctcgtc gaggtccggg tcggcccaca gcgcggcgcc gtccacgagg cccccgtcgc
      901 gctcggggag cggcccgatc cgctcgcgga tcgcggcgag ggcgagttct tcccgctccc
      961 gctcgccctg gatcagcgcc gccacgccgg cttcgtccag tcccagcgac tccgcgctca
     1021 tccagggctc gaggcggctg ccgccccacg acgcgtggat gatgccgatc ggcacgtcgt
     1081 ggtgctgccg gatctcgcgc gcgaagaaat acccgacggc cgtgaagccg ccgacgtgcc
     1141 cgggatccgc cggcgtccac ggcccgccga ccaccgagtc gacgggcgaa tccgaccacg
     1201 ccttcggcac caggaactgg cggatcatcg ggtcgtcggc cgacgcgatc tcggcctgcg
     1261 cgtcccaggc gctgtccacg gtccactcca tgttcgattg tcccgaggcg acccagacgt
     1321 cgccgaccag cacgtcacgg atcgtgcgct cgtacccctc gcccgaaatc ctcatctcgt
     1381 gcggcccgcc ggccggcgcg gcgtcgaggc ggatcatcca cgtgccgtcc ggccccgcgg
     1441 gcgcctggta gctcgcgccc aggaagtcga cggtcaccat ggcgtccggc ctcgcggtgc
     1501 cccagacgcg caggtccacg tcccgctgca ggaccattcc gtcgtcgaac aggcgcgacg
     1561 gcacgatgtc ggagggtgga gcggagcagg ccgcggcgga gaggatcgac agggtgccga
     1621 gcaccaggag caggacgtgc gagagcgagg ggcccagcgg gtccgctgcg tggagcgtca
     1681 tggaaggtcg gagttggaaa cgaaggcgac gaagcgcccg tcgggggacc aggagggcac
     1741 gttgatcgtc ccctgcccgc cgaatacgta ggcgatgatg cgcggctccc cgccgccggc
     1801 cggcatcatc ctcaggtaga ccggacggta gaaggggtgg tcggacgggt ccacgtccgc
     1861 catgaaggag aggaatacga cgcgcgagcc gtccggcgac acgtgcggaa accagtcgtt
     1921 gtacccgtcg cgggtcatgc gcgtcggctt cgatccgtcc gcccccatgc gccagagctg
     1981 catggtgccg ctgcggaccg agttgaacca gatcgaccgg ccgtccggcg cgtactcggg
     2041 cccgtcgtcg agccccgggg cgtccgtcaa ccggacctcg tccccgccgc cggccggaat
     2101 ccggtagatg tcgtagtcgc cgccgcgctg ggccgtgtac acgaggtggc gcccgtcggg
     2161 cgaccagccg tgcaggtacg acgggccgac cggcgtgacg gcgcgaggca ccccgcccga
     2221 ggcgggcacg gtgtagacga tcgactctcc gccgtggtcc tcgctgtggt ggctgatgcc
     2281 cagcatgctc ccgtcgaagg agaggacgtg gtcgttgttg ttgcgggtcg cgaaatcggt
     2341 gtcgatgggc gtcgcctcgc gcgaggccag gtcgaaccgg tacaggaggc cgtcctggtt
     2401 gaagatgagg gccttgccgt cgggggtcca gttcggggcc tggatcgatc ccttcgaacg
     2461 atggaacacg cgccggtgcc cggtatcgac ctccaggact tccaggcggc tcccgatgta
     2521 gtcccggtag ggcacccagc cggccggcgg cgggaccacg aggcgcacgt tgtcgaagtc
     2581 ggccgtctcg gcgacggcgt tgtcgtgcga gcacacgaag agcccgacgt acaggtcgtc
     2641 gccgagcgcc gggccgtcga gcgtctccgt gacgaacggc tcgccgaagc gcgccaccga
     2701 catgacgaac cggtttccgc gccgctcgag ctgcaccacg tccgggccgc ccgcggagga
     2761 gcggacctcc tcggtgttcg cccccgggcc ccgccggtac tgcatcgacg tcatcccgtc
     2821 gccgtgaacg gccacgtcca cgtagggaga cccggggtcg agcgacgacc gggcgatgac
     2881 tccgatcttg cgatgctgat ggcccccgtc cccgcggaag gcgacccgcg cccggatcgt
     2941 gaagtcgccg ctcacccggc gccacacgaa gcggaaggcg tccgagccga accacatgtt
     3001 ctcgccggag cccgtgaccg tgtaccgctg cgcgacggcg tcgtaggcga gcgcgccctc
     3061 cacgcccacc ggccctatgt cggtcgtgcc ctcgaaagtc cccagttcct ggcgcgcctg
     3121 cgcggcagga gagacggcca tccagagggc ggcgacgagc gggaggacca ggtcagcgcg
     3181 catcggcctg cagtttgacg gccgtgccgt aggccagcag ctccgccgag ccggccatca
     3241 cctgggcggt catgaaccgc acgttgatca cggcgtccgc gccgagccgc cgggcctcgg
     3301 cctccattcg ctggagcgcc tcgttgcgcg cctcggtcag catctcggtg tactccttga
     3361 tctccccgcc cacgagtgta cggagcccgg ccatgatgtc cttccccagg tgcttggccc
     3421 gtatggtgtt gccccggacc atgcccaggg tctctcgcac ggagtagccg tgcacgaagt
     3481 cggtgttgac gatgatcatg tatggggaat gaggtttgtt caaagtgtcc ggccgggccg
     3541 tcagcggtcg cgcagcgccc ggtcctcgcc gcgctcctcg acgcgctccg cgatgagcga
     3601 cgccatgacc aggaggaagc ccaggagcag ggtccccagg cccaggcgga tgggccaggg
     3661 cagctggagc aggaacggcc acacgtcgcg cagcgggcgg atcatccaga cgaggagcgt
     3721 caggacgccg acgccggaga gcaggtatcc taggatgcgg gtgaagcgga tcatggcgtg
     3781 gcggtgtcga ggtcgaaggg atcagcccgg ctcggggagc gcgtcgtaga attcctccat
     3841 ccggcggcgc atcgacgcat cgggcagttc gcccatggcc gcgcccatgt tgtcgcgggc
     3901 atggtgcggc ttcgtggtcc ccgggatggc gaccgtcacg gccgggtgcg acacgacgta
     3961 cttgaggaag aactgccccc acgaggcgat gccggcctcg gcggcccact cgggcactgg
     4021 ccggtcgccg acgcgcgcga acaggcgtcc gcgcccgaac ggcaggttta tgagcgaggc
     4081 catgccccgg tcggccgcca gcgggagaag gcgttcggcg gcgccgcggt tggccagcga
     4141 gtagtcgacc tggacgaagt cgagctcgtg ccgcttcatc gtggcctcga aatcctcgta
     4201 ctggcggctc gacgacgtcg tgatgcccac gtagcgcacg cggccggcct ccttccactc
     4261 gcgaagcgtg gccagctgcg tgtccacgtc ccggagattg tggacctgca tgaggtccac
     4321 cgagtcgccg cccaggagtc ccagcgaacg ctccatgcgc tcgatgcccg catcgcgccc
     4381 ctcctggtcc accttcgtgg acatgaacac cctggtgcgg gcctcaagcc gtgcgaggac
     4441 ccgtcccagg acctcctccg agttgccgta cgacggggcc gtgtcgatca cgcgtccgcc
     4501 ggcttcgagg aagacctcca tcgtgtccac gaacggcacg agctcgtccg ccccggcccc
     4561 ggcccggtac tcccgggtgc cgatgccgat gacgggcacc tcctcgccag acgacgcgat
     4621 gggcttcagg atcagcttcc gcgtcctcga ggacgccgga cgcacgccgg ccagcagggc
     4681 ggctcccgtg ccggcggacg cacggatgaa atcgcggcgg gtccacttca tcgcggcacc
     4741 ttcacatgct tgagggaccc acattgtaaa gggcgaggcc cgaagccgca atgcccgccc
     4801 ggccgccccc gggtcgccgc tcgtcaggcg gtgccgggct cggccgcggc cagcagctcg
     4861 tgccgccggc ccaggttcag gctcatcagc acccacagcg cggcgagcgg cgctgcgacc
     4921 agcgcgatgg ccccgaccga gagccccacg gccgccaggc cggcgtagat ccagccgctc
     4981 accaggtcgc cgccgcggta cacggcggcg tcgaggaagg ccttggactt gtaccgttcc
     5041 tcgcgggaga ccaccgtgaa gagcacctcg cggaccggct tcgcgatgcc gtagcggccc
     5101 gcccggtaca gcacctggag caccacgagc accacgagga gcgggaccag gcccaggccc
     5161 acgaagccga tcatcgtgag cgccggaacg atcatcaggc tgaatcccac gcccagccag
     5221 cgcaggatcc gggccgtcac ggccacctgg agcacgatcg tgaggatgtt caccgcgagg
     5281 tcgatctgcg caaagaacga ggcgcgcgcg gcccggtccg cgatcgcctc gccgaccagg
     5341 aaggcctgct ggaaat
//
LOCUS       DBNHQZ010000183         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3713634, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000183 DBNHQZ010000000
VERSION     DBNHQZ010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3713634"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="AC16YU_04845"
     CDS             complement(<1..1307)
                     /locus_tag="AC16YU_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6064584.1"
                     /translation="MRIPYLDRLRPRMLFPHTFRLFEEHLDWRLTGRWMLLSTAVGIV
                     VGIGALILSQLISLASSGMLMELVGYQMPVPGGEAPDGAVFDLARALEPTRRWLLFVV
                     PTLGGLVSGWLVFTFAPDAEGHGTDSVIHAFHRNRGIIGPRVPVVKTIASAITIGSGG
                     SAGREGPIAQIGGALASLLAQGLKLGDRDRRMLLVAGMAAGVGSIFLSPLGGAFFAVE
                     VLYRQDIETEALMPAVVAGVTGYSVYSTIEGTGAVFLAPDIAYVSPLELVPLVVFALL
                     CAAVGIVYVDVFHGVKRRIFDPMRVPRMVKPAVGGFAVGAVAFFFPPILGSSYGWLQQ
                     AMDGTLPIALMATLAGLKILSTSFTIGSGGSGGVFAPSLVIGGMIGGVFGAGMHTLVP
                     DIVVHPQAYVMIGMATLLAGVAKVPLSTTIMISEITGSYRLLVP"
     gene            1482..2756
                     /locus_tag="AC16YU_04850"
     CDS             1482..2756
                     /locus_tag="AC16YU_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR6064585.1"
                     /translation="MSSLPARRLSTPRPTLDLLHFAGALLGILVVVHLWIMVDRGFDR
                     GCFGFSEPTAFVECEIVTTSQAGRLFGVSNAIWGLLFYVAMVALSALAGLPRTGRSPV
                     WRRLRAAALAFGIVYSGYLVVLQATQIGEYCRLCMMSAAIVTFLFSLVLIELATEPSA
                     DRPPMRKPALFAALGLAAVVLAAADLSYFSGLAVVDVAPATESVARAASPATDDTGSD
                     GLELRDEPDAETAPPVAAASSACEWDDSIPPVEDYLSLVTFSDPFLGASTAPVMVYEF
                     FDPNCGHCRRLHPIMKQLAASHGTTARIYPIPFMLWPDRSLLPIEALYVAAQDGKYHE
                     MMEAQFAGATGKGYFTVPELLQMAVDIGLDEASFTERLRRGLNRDIIWSRRVQIAELG
                     IRSTPTVMINGRLVRTKSLDCLIQFVEEAAAG"
BASE COUNT          456 a          976 c          934 g          421 t
ORIGIN      
        1 ggaacgagca ggcgatagct ccccgtgatc tccgagatca tgatcgtggt cgagagcgga
       61 accttggcga cgccggcgag cagcgtggcc atgccgatca tgacgtaggc ctgggggtgg
      121 acgacgatgt ccggcaccag ggtgtgcatt cccgccccga agacgccgcc gatcatcccg
      181 ccgatcacca gcgacggggc gaacacgccg cccgatccgc cggatccgat cgtgaaggac
      241 gtggacagga ttttcaggcc ggcgagggtc gccatgagcg cgatcggcag ggtgccgtcc
      301 atggcctgct gcagccagcc gtaactcgat cccaggatgg gcgggaaaaa gaacgcgacc
      361 gcgccgacgg cgaagccgcc cacggccggc ttgaccatcc tcgggacccg catcgggtcg
      421 aagatccggc gcttcacgcc gtggaacacg tccacgtaca cgatgccgac cgcggcgcac
      481 aggagcgcga acacgacgag cggcacgagt tcgagcgggc tcacgtacgc gatgtcgggc
      541 gccaggaaca cggcccccgt gccctcgatg gtcgagtaga ccgagtagcc ggtcaccccg
      601 gcgacgacgg ccggcatgag cgcctccgtc tcgatgtcct gccggtacag gacctcgacc
      661 gcgaagaagg cgcccccgag cggagacagg aagatgctgc ccacgcccgc cgccatgccg
      721 gcgacgagca gcatccgccg gtcgcggtcg ccgagcttga gtccctgcgc gaggagcgac
      781 gcgagggccc ctccgatctg cgcgatcggg ccctcccggc cggcgctgcc tccggatccg
      841 atcgtgatgg ccgacgcgat ggtcttgacc acggggaccc gcggaccgat gatgccccgg
      901 ttacggtgga aggcatggat caccgaatcc gtgccgtgcc cctcggcatc gggcgcgaac
      961 gtgaacacga gccaccccga gacgagtccg cccagtgtcg gcaccacgaa cagcagccat
     1021 cgccgggtcg gctccagggc gcgcgccagg tcgaagaccg cgccgtccgg ggcctcgcct
     1081 cccggaacgg gcatctggta gcccacgagt tccatgagca tgccactcga ggccagcgag
     1141 atcagctggg acaggatcag ggctccgatg ccgaccacga tgccgaccgc ggtcgagagg
     1201 agcatccacc gcccggtgag ccgccagtcc aggtgttcct cgaacaggcg gaacgtatgg
     1261 gggaagagca tcctcggccg gagccggtcc aggtagggaa tccgcatcgg aggaagtgga
     1321 atgccgcaag gtccgaagcc cgaggcaagc ggtcaaacga gcgggggcgc cgactcctgt
     1381 cggggaaacc ccatggcgcg cccgaagccg gatcaaagcc cggacggaac ggtttccttc
     1441 cgcgcatcgg agcctgcggc cccaacaacc gattccggaa gatgtcgtcc ctgcccgcac
     1501 gtcgcctgtc gaccccgcgc ccgacgctgg acctccttca ctttgccggt gccctgctgg
     1561 gcatcctcgt ggtcgtgcac ctgtggatca tggtggaccg gggtttcgac cggggctgct
     1621 tcgggttcag tgagcccacg gccttcgtgg aatgcgaaat cgtcaccacg agccaggccg
     1681 gaaggctctt cggcgtgtcc aacgccatct gggggctgct cttctacgtg gcgatggtcg
     1741 ccctgtccgc gctcgccggc ttgccccgga ccggccggtc gcccgtgtgg cgcaggctcc
     1801 gggccgccgc gctggctttc gggatcgtct acagcggata cctggtggtc ctccaggcca
     1861 cccagatcgg cgaatactgc cggctgtgca tgatgtccgc cgccatcgtc actttcctgt
     1921 tctccctcgt tctcatcgaa ctggcaaccg aaccgtccgc cgaccgtccg cccatgagaa
     1981 agcctgccct cttcgccgcg ctcggccttg cagcggtcgt cctcgcggcg gccgacctgt
     2041 cgtacttctc cggactggcc gtcgtggacg tcgcgccggc caccgaatcc gtcgcccggg
     2101 ccgcgtcgcc cgcgacggac gacacgggtt ccgacggcct cgagctcagg gacgagccgg
     2161 atgcggagac cgccccgccc gtagccgccg cttcgtcggc gtgcgagtgg gacgattcga
     2221 tcccgcccgt ggaggactac ctgtcgctcg tcaccttcag cgatcccttc ctgggcgcct
     2281 cgaccgcgcc ggtcatggtg tacgagttct tcgacccgaa ctgcgggcac tgccggcggc
     2341 tgcatccgat catgaagcaa ctggcggcgt cgcacggcac gacggcgcgc atctacccga
     2401 tcccgttcat gctgtggccc gaccggtcgc tgctcccgat cgaggcgctg tacgtcgcgg
     2461 cgcaggacgg aaagtaccac gagatgatgg aggcgcagtt cgccggggcc accggcaagg
     2521 gctacttcac ggtgcccgag cttctgcaga tggccgtcga catcggcctg gacgaggctt
     2581 cgttcacgga acggctccgc cgcggcctga accgcgacat catctggagc cgccgcgtgc
     2641 agatcgcgga gctcggcatc cggtcgactc cgacggtcat gatcaacggg cgcctggtcc
     2701 gaacgaagtc cctggactgc ctcatccagt tcgtcgagga ggccgcggcc ggctgacggg
     2761 gttttcgcag gatttctgat tcgtgcg
//
LOCUS       DBNHQZ010000184         5475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3169656, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000184 DBNHQZ010000000
VERSION     DBNHQZ010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3169656"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1642)
                     /locus_tag="AC16YU_04855"
     CDS             complement(116..1642)
                     /locus_tag="AC16YU_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR6064586.1"
                     /translation="MSDPLARLLERLATVRDIEAAAALLDWDQETHMPPAGTEARARQ
                     VSTLQRLAHELFTADETGTLLEAARPDSELDVDLVRVTRRDFERGRRIPADLVADLAR
                     ETSRAKDAWRLAREADDFGRFEPHLARIVELCIRKAEALGFENTPYDALLDEYEPDLT
                     AAYLDQVFSDLQRELVPLVRVLTQASRDESRSLLHGHFPDAAQWAFGLAVLRDIGFDF
                     DSGRLDRSAHPFTTAFSISDVRLTTRVHEDRFASAFFGTLHEAGHGLYEQGMDPALEG
                     TPLADGASLGMHESQSRLWENQVGRSRPFWECYLPRLREVMPGPFDGVDLDAFLRAVN
                     RVRPSPVRIEADEVTYNLHIMLRFSLERDLVEGRLAPGEVPGAWNAGMESLLGVAPPS
                     DADGCLQDIHWSLGILGYFPTYALGTLMSAQLWAAIRHDLPDLDDRIRRGDFAPLLGW
                     LRERVHRHGRRRSAAQILHDATGEVLSANAWLGYVRTRYGGLFGADLPAAATWPSPGS
                     "
     gene            complement(1639..2955)
                     /locus_tag="AC16YU_04860"
     CDS             complement(1639..2955)
                     /locus_tag="AC16YU_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064587.1"
                     /translation="MSTPVRRLAFVLPVLLAAAVAAYAPPQPMAPAAPPDPVNGVTVR
                     GTTVTVERPNMLLDRLMASPDGAAAHSALSGLLESGLSGLVPGGDNTGGDDGGGLGSL
                     ADRARSALGSLTGQTSEAADEVTSSGGEKTSTEDFELHIAGDRFRMDQGSHSVIILAE
                     DDGVRYWFVYHDARRIWGMHAAAATEAGRVTASSHGGMEISPGPSPETLLGHAAKHYT
                     FEFETTSDVAASLGADGQGPAMDVVVENRGEAWIADGIEGSDEIAAFYRSFAGGLSGL
                     ESGGGLQSGLVGGMADLADLGVPLKVVQETAVRLVTGAELGGDDQPMAWTRATTTVTG
                     IERGELDDEIFSVDAGGLADYERGMMPGFGGTGQAGAAAGGAAPGQATGDAVEEGCDC
                     SCAAYAELQDMSRDKKAAESDPRAMKLAMCARECAMKWVACARKSP"
     gene            3111..3183
                     /locus_tag="AC16YU_04865"
     tRNA            3111..3183
                     /locus_tag="AC16YU_04865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3144..3146,aa:Thr,seq:cgt)
     gene            3349..3612
                     /locus_tag="AC16YU_04870"
     CDS             3349..3612
                     /locus_tag="AC16YU_04870"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR6064588.1"
                     /translation="MEFEFGWDRVSRGMHFTVTVMVFLGSVFSSNWIVVADSWMQKGA
                     GDDPTYQQWQYPHPPCVIGAYVRTIRVAGRRHLPDRSPCSAEA"
     gene            3609..4892
                     /locus_tag="AC16YU_04875"
     CDS             3609..4892
                     /locus_tag="AC16YU_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064589.1"
                     /translation="MKRLVVLLSVFALPNLAVAQPDPGGIRLFLDCSWQCDDEFIREQ
                     IPIVDFVRDRTEADIHLLVTTEGTGGGGDVYTLAFLGRRRFEGVSDTLQVAVSGTDSS
                     DEIRREITKAIQLGLGPYLVRADLTGDVGITFGKPSAQAPASIETDPWNYWVFSVGSN
                     ASLQGQETSNYLSASGNLSANRTTEDLKVRLGVNGRFNEQNFEIGDETVTSRTRSGSL
                     SGLVVKSIGSNWAAGLSADAGTSTYSNTDVSLSVGPALEYNIFPYSESTRRELRVQYG
                     VELESFWYEEETIFNLTTERLLKHYVSVALDLKQPWGNLTFSTRGTHLLTNFDRSLLD
                     AYNVSVFAGANVRIVRGLSFNFFGNYSRIRDQLSLPLSDATEEEILLQSRRLPTGYDY
                     YFSFGFSYRFGSIFNNVVNPRFGSSGGGGMMIIMG"
     gene            complement(4899..>5475)
                     /locus_tag="AC16YU_04880"
     CDS             complement(4899..>5475)
                     /locus_tag="AC16YU_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6064590.1"
                     /translation="EAEKAFPGRTTIVRPGLIVGPGDPTDRFTYWPVRIDRGGEVLVP
                     GTGGDSVQFIDARDLVRFIVTLAENDVSGVFNATGPETRLSMAELVHGLRAVTTGPIR
                     FTWLDADFLEAQGVRPWSDMPVWIPGDPVSYVRLDRALEAGLTFRSLADTARDTLGWH
                     ASRPEEQRSRLRSGLSPEREAEVLAAWHARG"
BASE COUNT          848 a         1856 c         1901 g          870 t
ORIGIN      
        1 atgcacggac tcctgactgc tacatgtcca atgtcggtcg tgggtggcgg atcgttaacg
       61 ggcaggatcg cgaacgaccg taggccagca tcccggagtg ccggcgggat attccttacg
      121 aaccagggct gggccaggtt gccgcggcgg gcaggtccgc gccgaaaagc ccgccgtacc
      181 gggtccgcac gtatccgagc caggcgttcg cggacagaac ctcgccggtc gcgtcgtgca
      241 ggatctgcgc ggccgaccgt ctgcgcccgt gccggtgcac ccgttcgcgg agccagccga
      301 ggagcggagc gaagtcccct cgacggatcc ggtcgtccag atcgggcaga tcgtgccgga
      361 tcgccgccca cagttgggcg gacatcaggg tgccgagagc gtacgtgggg aaatagccca
      421 ggatgccaag cgaccagtgg atgtcctgca ggcagccgtc cgcgtcggag ggcggcgcga
      481 cgcccagaag ggactccatc ccggcgttcc aggccccggg cacttcgccc ggcgcgagtc
      541 gtccctcgac caggtcgcgc tccagcgaga aacggagcat gatatgcagg ttgtaggtca
      601 cctcgtcggc ctcgatgcgg acgggcgacg gacgcacccg gttcacggcc cgcaggaagg
      661 cgtcgaggtc cacgccgtcg aacgggcccg gcatgacctc gcggagcctg ggcaggtagc
      721 actcccagaa gggccgcgac cggccgacct ggttctccca cagccgggac tgcgactcgt
      781 gcatgccgag gctcgccccg tccgcgagcg gtgtgccctc gagcgcaggg tccatgccct
      841 gctcgtagag cccgtggccg gcctcgtgga gcgtgccgaa gaaggcgctc gcgaagcggt
      901 cctcgtggac gcgcgtcgtg aggcggacat ccgagatcga aaaggccgtc gtgaaagggt
      961 gcgccgaccg gtccagccga cccgaatcga aatcgaaccc gatgtcgcgc agcacggcga
     1021 gaccgaacgc ccactgcgcc gcgtcgggga agtgtccgtg cagaaggctc cgggattcgt
     1081 cccgggacgc ttgcgtcagc acccgcacga ggggcacgag ttcgcgctgc aggtccgaga
     1141 acacctggtc caggtacgca gccgtcaggt cgggctcgta ctcgtcgagg agggcgtcgt
     1201 agggagtatt ctcgaagccg agcgcctcgg ccttacggat gcacagctct acgatccgcg
     1261 ccaggtgcgg ctcgaaccgg ccgaagtcgt cggcctcgcg ggccaggcgc caggcgtcct
     1321 tcgcgcgcga ggtctcgcgg gcgagatcgg ccacgaggtc cgccggaata cgccggccgc
     1381 gttcgaagtc gcggcgcgtc acccgcacca ggtcgacgtc cagttccgag tccggacggg
     1441 ccgcttccag gagcgtgccc gtctcgtcgg ccgtgaagag ctcgtgcgcc aggcgctgga
     1501 gcgtggacac ctggcgcgct cgcgcctcgg tgcccgccgg gggcatgtgg gtttcctggt
     1561 cccagtccag aagggctgcc gcggcctcga tgtcgcgcac cgtggcgagc cgttcgagca
     1621 gccgggccag gggatcgctc acgggctctt gcgcgcgcag gcgacccact tcatggcgca
     1681 ttcccgggcg cacatggcca gcttcatggc ccgcgggtcg gactcggcgg ccttcttgtc
     1741 ccggctcatg tcctggagct cggcgtaggc cgcgcagctg cagtcgcagc cctcctcgac
     1801 ggcgtcgccg gtggcctgtc ccggggcggc gcctcccgcg gccgctccgg cctggcccgt
     1861 gcctccgaat cccggcatca tgccgcgttc gtagtcggcc aggccgcccg cgtccacgct
     1921 gaagatctcg tcgtccagct cgccgcgctc gatgcccgtc acggtggtcg tggccctggt
     1981 ccaggccatc ggctggtcat cgccgccgag ctccgcaccc gtgacgagcc ggaccgccgt
     2041 ctcctgcacc accttcagcg ggacgcccag gtcggcgaga tcggccattc ctccgaccag
     2101 cccggactgg agcccgccgc cggactcgag gcccgagagg ccgccggcga agctgcggta
     2161 gaaggcggcg atctcgtccg agccctcgat gccgtccgcg atccacgcct cgccgcggtt
     2221 ctccacgacg acgtccatcg ccggaccctg tccgtcggcg ccgagcgagg cagcgacgtc
     2281 gctcgtcgtc tcgaactcga aggtgtagtg cttcgccgcg tggccaagga gggtctcagg
     2341 gctcggaccc ggagagatct ccatgcctcc gtggctcgat gccgtgaccc gtccggcctc
     2401 ggttgcggcc gcggcgtgca tgccccagat cctgcgagcg tcatggtaca cgaaccagta
     2461 gcgaacgccg tcgtcctcgg ccaggatgat cacgctgtgg gacccctggt ccatgcggaa
     2521 ccggtctccg gcaatgtgga gttcgaagtc ctcggtggac gtcttctcac cgcccgagga
     2581 cgtgacctcg tccgcggctt cgctggtctg cccggtgagt gaaccgagcg cgctgcgggc
     2641 gcggtcggcc aggctgccca ggccgccacc gtcgtccccg ccggtgttgt caccgccggg
     2701 cacgagcccc gagaggccgg actccaggag cccggacagg gccgagtgcg cggcggcgcc
     2761 gtccggcgac gccatgagcc ggtcgagaag catgttcggg cgctcgacgg tcacggtcgt
     2821 gccgcgcacc gtgaccccgt tgacgggatc cggcggggcg gcgggggcca tcggctgcgg
     2881 gggggcatac gcggcgaccg ccgcggcgag cagcacggga aggacgaaag caaggcgacg
     2941 aacgggggtg gacatcgcga cctcggggtt gggtagcacg ggaaataaac gggggtatcg
     3001 gtatacgcgc aacccggtcg cgcggcagga cacggggccc gccggatccg cgccgccggg
     3061 ccgcgacatt gacacgggtg gcccggaagc cctatttttc ttttctgcag gccaatgtag
     3121 ctcagctggt agagcagctc actcgtaatg agcaggtcag cggttcgaat ccgctcattg
     3181 gctccgagag gtcgcgtggc gccccatccg gctccggagg gggcgctgtc ttttccggtc
     3241 gggacgcttt gcgccgcctc caccgcgtct tcaccgaatc cgtccggacg cgcggggtag
     3301 cacgctgacg gattaccgaa cacccgggcc ctgcgtccgg ggtgaacgat ggagttcgag
     3361 ttcggctggg atcgagtctc caggggcatg cacttcacgg tgacagtcat ggtattcctg
     3421 gggagcgtct tttcgtccaa ctggattgtt gtcgcagact cgtggatgca gaagggtgcc
     3481 ggggatgacc ctacgtacca acagtggcaa tatccgcacc caccttgcgt cattggtgcg
     3541 tacgttcgga ccatccgcgt cgccgggagg cgtcatttgc cggatcgatc accatgcagc
     3601 gccgaggcat gaaacggctt gtcgtactac tgtccgtttt cgctctcccg aatctcgccg
     3661 ttgcgcagcc cgatccgggc ggcatccgcc tcttcctcga ctgctcgtgg cagtgtgacg
     3721 acgagttcat ccgggagcag atcccgatcg tcgatttcgt gcgggaccgg accgaggcgg
     3781 acatccacct cctggtgacc accgagggga cgggcggagg aggcgacgtc tacacgctgg
     3841 cctttctcgg ccggcgccga ttcgaagggg tttccgacac gctccaggtc gccgtgtcag
     3901 gcaccgattc gtccgacgag attcggcgcg agatcacgaa ggcgatccag cttggcctcg
     3961 gtccgtacct ggtgagggcg gacctgaccg gggacgtggg catcacgttc ggaaagccct
     4021 cggcccaggc accggcctcg atcgagaccg atccgtggaa ctactgggtg ttcagtgtcg
     4081 ggtcgaacgc cagcctgcag ggtcaggaga cgtcgaacta tctcagcgcg agcggcaacc
     4141 tgtcggcgaa ccgcacgacg gaggacctca aggtccggct gggtgtgaac ggacgcttca
     4201 acgagcagaa tttcgagatc ggcgacgaaa cggtgacgag ccggacgcgg agcggcagtc
     4261 tgtccggtct cgttgtgaag agcatcggct cgaactgggc tgccgggctc agcgcggacg
     4321 cgggcacgtc gacctactcg aacacggacg tgtcgctttc ggtcggtccg gctctcgaat
     4381 acaacatctt tccgtacagc gagtcgaccc ggcgcgagct gcgcgtgcag tacggcgtgg
     4441 agctggagtc tttctggtac gaggaggaga cgattttcaa cctgacgacc gagcggctgc
     4501 tgaagcacta cgtctccgtc gccctggacc tcaagcagcc ctggggcaac cttacgtttt
     4561 cgacgagggg cacccatctg ctgacgaact tcgaccgctc gctgctcgac gcctacaacg
     4621 tgtccgtctt cgccggggcg aatgtgcgga tcgtgcgcgg actctcgttc aacttcttcg
     4681 gcaactactc caggatccgc gaccagctct cgcttccgct ctcggacgct accgaggagg
     4741 agatccttct gcagagccgg cgcctgccga cgggctacga ctactacttc tcgttcggct
     4801 tcagctaccg cttcggctcg atcttcaaca acgttgtcaa cccgcgcttc ggtagtagcg
     4861 ggggcggtgg catgatgatc atcatggggt gatgcggctc agccccgcgc gtgccaggcg
     4921 gccagcacct cggcctcgcg ctcgggcgac agccccgacc gcagccgcga gcgctgctcc
     4981 tcgggccggg aggcgtgcca cccgagcgtg tcgcgcgcgg tgtcggccag tgaccggaag
     5041 gtgagtcctg cctccagggc gcggtccagc cgcacgtacg atacggggtc cccgggaatc
     5101 cacaccggca tgtcggacca cgggcgaacg ccctgggcct cgaggaaatc ggcgtccagc
     5161 caggtgaacc ggatcggtcc cgtggtcacg gcgcgcagcc cgtggacgag ctcggccatg
     5221 gacaggcgcg tctcggggcc ggtcgcgttg aagacgcccg acacgtcgtt ctcggcgagc
     5281 gtcacgatga aacgcacgag gtcgcgggcg tcgatgaact gcaccgagtc cccccccgtg
     5341 ccggggacca gcacctcgcc gccgcggtcg atgcgcacgg gccagtacgt gaaccggtcc
     5401 gtcgggtcgc cggggcccac gatgagcccg gggcggacga tcgtcgtccg gcccgggaac
     5461 gccttctcgg cctcc
//
LOCUS       DBNHQZ010000185        30781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2086451, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000185 DBNHQZ010000000
VERSION     DBNHQZ010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 30781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30781
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2086451"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..727
                     /locus_tag="AC16YU_04885"
     CDS             122..727
                     /locus_tag="AC16YU_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR6064591.1"
                     /translation="MDAMTRAEAGKWVTAQIDEAATVQEAVRSMAGQLVEIAFAISDR
                     LSAGGQVLFFGNGGSAADAQHWAAELSGGFYRRRPALPAAALTANSSQVTAIANDLGF
                     EEVFARPVHALGRPGDVAVGISTSGRSPNVIVALEAARSRGMMTVGFTGRDGGAMAPI
                     CDLLISIPSDDVARIQEGHELCAHAVCAVIEHLQFPDAERG"
     gene            720..1292
                     /locus_tag="AC16YU_04890"
     CDS             720..1292
                     /locus_tag="AC16YU_04890"
                     /EC_number="3.1.3.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034200 - D-glycero-beta-D-manno-heptose
                     1,7-bisphosphate 7-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-alpha-D-manno-heptose-1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR6064592.1"
                     /translation="MVDRRAAVFLDRDGTLNEEVEYLHRPEDFRWIRGAPESIQALNR
                     AGLLVIVITNQAGVAHGRYDEAAVAHLHESMQADLAHRGARIDAFYTCFTHPDARIEK
                     YRRDDPCRKPGKGMFLHAIDDWSVDAGRSFMVGDKNTDLVPARELGMRTILVRTGYGA
                     SMESTTVADLVESDIAAAVKHILGQSTTTR"
     gene            1313..2086
                     /locus_tag="AC16YU_04895"
     CDS             1313..2086
                     /locus_tag="AC16YU_04895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6064593.1"
                     /translation="MERHPLLQRIFFSLLHVVFLRSWYVRRALRDAVPRDGSPIRLLD
                     AGTGFGQYAWHVLRRYPHVRVHAVDVKAEYLENLGRFVASTGRADRLTLAVDDLTRME
                     SEGPFDVILSVDVMEHIEDDRAVFRNFREIAAPGGRVLINTPSDQGGSGVTAEGGASF
                     IGEHVRDGYAADELCMKLRDAGLEIESVTYTYGSAGSLAWRMLLQVPMLLLAKGFWTL
                     PLVALWYLPAFPMGMILNAVDVLRDNRTGTGLLVVARRP"
     gene            2230..3927
                     /locus_tag="AC16YU_04900"
     CDS             2230..3927
                     /locus_tag="AC16YU_04900"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR6064594.1"
                     /translation="MQTKYIFVTGGVTSSLGKGIVCASLGRLLEARGLRVTIQKFDPY
                     INVDPGTMNPYEHGEVFVTDDGAETDLDLGHYERFLGVPTSQANNVTTGRIYLEVITK
                     ERAGAYLGKTVQVVPHIIDEIKHWLMKLGGTGEYDVVITEIGGTVGDIEGQPYLEALR
                     QVRYELGSRNVLNIHLTLVPYLEAAGEVKTKPTQHSVKTLLSFGLQPDVLVCRTDRPL
                     DLDVRRKLALFCNVEPKAVIASRDAESIYEVPLLLKEEGLDEIVMDRLRLAEDAEPGR
                     VTDPDLSDWIGFLKRLKEPGATVRVALVGKYVTHQDAYKSINESFILAGSRHGVAVEV
                     KQVLSDSLTEENVAEVLGDCSGIVVAPGFGDRGIDGKLVAVRFARENDVPFFGICLGM
                     QCAVVEFARNVCGWDRAHSTEFDADTPYPVIDLMNEQKMIADKGGTMRLGAYDCSLAE
                     GSLARGIYGQAEVRERHRHRYELNNVLRYKLAEHGMYFTGINRSRDLVEIVELPDRRW
                     FVGVQFHPEYKSTVGRPHPLFLSFVEAVIGHARDQDLIKSPKPVRREKKLQLASAKV"
     gene            4186..4629
                     /gene="mraZ"
                     /locus_tag="AC16YU_04905"
     CDS             4186..4629
                     /gene="mraZ"
                     /locus_tag="AC16YU_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6064595.1"
                     /translation="MASFKGQADYSVDGKGRVAIPAKMRAALSPEAKGTFTMTRGFER
                     CIVLYPLDEWERIEADIANLNMYNRQSRDFARIILMWADEVALDAQGRVPLTRPLMDF
                     AGIRERALIIGALDHVEIWAPDAFATYEKEQSADYETLAERVMGG"
     gene            4631..5635
                     /gene="rsmH"
                     /locus_tag="AC16YU_04910"
     CDS             4631..5635
                     /gene="rsmH"
                     /locus_tag="AC16YU_04910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6064596.1"
                     /translation="MPPSDELDTSVPGAEGDDPFATGYHAPVLCMAVVSGLILRRDGL
                     YVDATLGGGGHTAALLEALGPGGRVIAIDRDGDAIREAGRRLEPEVGKGRLRLVRGNF
                     SELETILAGFGESRVDGVLLDLGVSSHQLDLGSRGFSFRIDGPLDMRMDDRAGATAAD
                     LVNGLDEAGLRRLLRIYGEEPSAARIARAVVAARPVLTTTGLAEAVRGAVPGARNSAK
                     ALARVFQALRIAVNAELDALEDAIRAATRQVRVGGRLAVISYHSLEDRRVKRFIRSGN
                     FEGRLERDLYGNPVVPWREVHRHALKPDEEEIVRNPRARSARLRIAERISEDRPTGSV
                     "
     gene            5802..6143
                     /locus_tag="AC16YU_04915"
     CDS             5802..6143
                     /locus_tag="AC16YU_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064597.1"
                     /translation="MPGWGELEKGNRREGVRKGGWDVLLDTVPTIRLSLYILGVAILV
                     TLYVGHVHSTQRLAERVQNMSRTNLSLHLRHNRVKGAFDRASGPAVIYERARRLGLSE
                     SLPNGYTVITD"
     gene            6187..8268
                     /locus_tag="AC16YU_04920"
     CDS             6187..8268
                     /locus_tag="AC16YU_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6064598.1"
                     /translation="MHVKDEILARMYVLLTLLAVVPLLVAVQVVRITLIQGAELREQG
                     ERQATSQFTIPAMRGTIVDRHGRTLAVNTARYDIAVDPTIPGYRDRAPAFFDRLARTT
                     GRSPAWYRTLVARSSSRQYARLERGIDESALDALREIDLPGLIVQSRFSRRYTYGTAA
                     AHVLGHVDTDLHGIAGIESRYEPVLAGIDGRQSAQRDRRGVARASADGMTVEPVHGET
                     VVLTIDLVRQAVLEEELARGVAATGAKWGSAIAMDPWTGAVLAMANVPTYDANDPGAY
                     SHPEMRNHAVTDQLEPGSTFKLVTAVAAIEEDAASLDDSLETGDGWIVLHGRTLRDTH
                     ALGTVTFRDVIAHSSNVGIALVAERLKAADFHRHASSLGFGRKTGIDLPGEVAGVLKD
                     PKNWSGTTLTSMSRGYEVAATPLQVLVAYAALANGGTLVRPYVVAERRDVTGNVVWTA
                     SQDSLRRAFRRSTARTLIPAFEQVVTDGSAREAAVPGVRIAGKTGTARKVRDGSYESG
                     AYRASFVGFYPVEDPRVVLIVVLDEPRTSYYGGSAAAPVFRETVRRWLATTDDAPVGT
                     LAEAPDPDDIEIPSPDVVRLPLALAADRVRAYGLRTSVSDDGPRLTVTSQRPEPGAAL
                     RQGSRVRLAAEFGDATEAMPDLRGLTVREAVWWLTSLGITPRIEGSGTVRSQSPNPGS
                     PLGQTAVLRCR"
     gene            8273..9775
                     /locus_tag="AC16YU_04925"
     CDS             8273..9775
                     /locus_tag="AC16YU_04925"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR6064599.1"
                     /translation="MTGRSVPITFSPQPLATLVDRLRRAGLVLSAEDAGDGIVDHLAF
                     DSREVGPGGLFVAIRGEHTDGHLFIDRAVQNGAAAIACEAAPAGDWPAGVARVRVSDS
                     PRALAELACAAFGDPSRALRTIGVTGTNGKTTTAWLCAQGLDGVGLPCGYLGTLGSGR
                     VQRMSPGTHTTPDAVSLQAFLRGLADEACAAAALEVSSHALVQRRTHGIRFEVGVFTN
                     LTRDHLDYHTDFESYASAKRLLFQSMAADSTAVVNADDPAAASVVGGTPPRQVRYGTG
                     TDADVRYAVRGDGIGGLRLHLDGHDRRFRLSGAFNASNLAAAYATLVALGVDGSEAAE
                     SLSEASPAPGRFEPVAAPGCADFLVDYAHTPDALAHALAAARKLVGRDGRLWCVFGCG
                     GDRDAGKRPQMGAVAEAGADRVVVTSDNPRTEDPERILEDVRSGVSRPEDMLWIVDRR
                     EAIHAVARLADESDVVVVAGKGHETYQVVGEERLPFDDRDVILEACNQVA"
     gene            9779..10936
                     /gene="mraY"
                     /locus_tag="AC16YU_04930"
     CDS             9779..10936
                     /gene="mraY"
                     /locus_tag="AC16YU_04930"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR6064600.1"
                     /translation="MLYYLILWLEREFQPPGFQAFQFITVRASLSAITALFIALIIGR
                     RIIRWLERQQIGEQVRQGVSAGAVDHSHKAGTPTMGGLIILFAVLGATLLWGAMAEVY
                     VWLMVVATAWMGAFGFADDYIKVVKRDKSGLPARIKLIGQVSLGVFVGCVIYFHPAFR
                     DVHTLTYLPFVADQTLDYNVFGSWFGGVDLGWVIYIPVVVFIITALSNAVNLTDGLDG
                     LAAGVTGIVALGLTALAYIAGNAVFANFLNEMLLPGAGELTVFASALAASCFGFLWYN
                     GYPAQVFMGDTGALSLGAAIGTLTLLVRKELLLPLLAAVFFIEAVSVIIQTTWFRHTR
                     RKTGTGRRVFRMAPLHHHFEVGGMHEAKIVVRFWLITAFTVIATLLLLRIR"
     gene            10939..12327
                     /gene="murD"
                     /locus_tag="AC16YU_04935"
     CDS             10939..12327
                     /gene="murD"
                     /locus_tag="AC16YU_04935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6064601.1"
                     /translation="MIAPLSEMISPKLRHESVSVVGAARSGVAVSLLLAGYGARVFVT
                     DRADIPRTTLDLLARHGVKAESGGHSDRALGADLLVVSPGVPTTAPLVMEAMERGIPV
                     RSELEVASWFCEAPIVAVTGSNGKTTTTALVGHVFRTAGRTTWVAGNIGLPFSSVASR
                     AGQDDVVVLEVSSFQLDHTESFRPRVSVLLNITPDHLDRYGHDLEAYAAAKLRIAERQ
                     GAGDAVVYNADDPILRRFAESLDAGSGPTPLGVTLGTPDARSAAWLTEDRLTLRIQDT
                     QEDFMRAEELALRGRHNLYNSLAAAVAARVMEVRSDVVRASLMSFEGIPHRLETVREL
                     DGVKYVNDSKATNVNAVWYALESFREPVLLIAGGRDKGNDYEPLKRVVQDRVRVLLAI
                     GESADHVVRELGPHAGECFRAGTLDDAVQMARTLARAGDVVLLSPACASFDQFDNFED
                     RGDTFRRLVADL"
     gene            12332..13471
                     /locus_tag="AC16YU_04940"
     CDS             12332..13471
                     /locus_tag="AC16YU_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6064602.1"
                     /translation="MATAATSRLKIRPADKYVVWIVLILAAVGVVAVYSAISFLAQTR
                     AGGDTEQFLVRHLLRLGLAFGVMVAVSLVDYRKLARIARLALLGSFGLLLLVQVVGTT
                     SGGAARWLQFGSFGFQPSDIARVSLVLYVGTLLAAKQAYIKEFGRGFAPILVWILASV
                     MLIGLEDLSTAAVVLAAVGAMCFVGRVHVAQLAGVALLGLLLAYMMLLSSPHRAARIE
                     SYVGVRLFPNTEGIDVFSAQEEGFQAHQARIAFAMGGLTGAGPGKSTQRDFLPAPYND
                     FIFAIIAEEYGLVGSMGLLALFVLLLAQGFLRIARHSPDPFGLFLAVGLTVMLVLYGF
                     VHAGVASGLLPVTGLPLPFVSYGGTSMVANGAMVGILLNISRAVR"
     gene            13474..14589
                     /gene="murG"
                     /locus_tag="AC16YU_04945"
     CDS             13474..14589
                     /gene="murG"
                     /locus_tag="AC16YU_04945"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR6064603.1"
                     /translation="MPRPPRILLAGGGTGGHVYPAIAIADAIRRTEPESVVAFAGTTD
                     RMEWDAVPKAGYAIHPIAAAGIRRSLSLRNLRVPLTMARGLAQSWHLVGDFDADAVVG
                     TGGYVSGPVGLAAAMRRRALVVQEQNARPGVTNRILGRMADRIHVAFDEAWAAFPPGR
                     TTVSGNPIRPGLAAMGRDEARERYGIPAEARVLLVLGGSLGSQAINEAVARHVGRLLE
                     SPDTVVIWQAGSRYVERMAAAVPGHERLTLSAFIDRMDLAYAAADLVLCRSGAITCSE
                     LLATGRPSVLVPSPNVTEDHQTRNAESLVRIGAARLLAESGLAEGWEPLVRQLLHDPE
                     TLASMAGAARSHAVLDSADRVARDVLDLARHRAARAA"
     gene            14586..16016
                     /gene="murC"
                     /locus_tag="AC16YU_04950"
     CDS             14586..16016
                     /gene="murC"
                     /locus_tag="AC16YU_04950"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6064604.1"
                     /translation="MTLPRRQPSFGRIRRVHMVGIGGIGMSSIAEVLLTRGFEVSGSD
                     LQRTDITDRLASLGARIFEGHAAANVEGAHVVVHSSAVHAESNPETIEAARQRIPIIG
                     RAVMLGELMRTKYGIGIAGTHGKTTTTSMAGLVVTEGGFDPTIIVGGKVSVFGSNAVA
                     GEGDIIIIEADEYDRTFLRLTPSLAVITNIEEEHLDIYEGIDDLLDAFTRFAGSVPFF
                     GAAICCLDDPNVQRIVGSIDRRVVTYGISRQASVRADRVRQDGLAMQFDVLQEGRRLG
                     SVDLAVPGAHNVLNALAAVAVGLELDIPFERIRAGLGRFSGVQRRLHRIGEAGGILVV
                     DDYAHHPTEIRATLQTAARSWPDRRIVAVFQPHLYSRTRDFLDEFARAFFDADVVFVT
                     DVYAARETEMDGVNGGQVAERARAFGHRAAHYEPDREALPALVARMAQPGDLVITLGA
                     GDIWRFSRALLRELGAETGAETREEA"
     gene            16020..16772
                     /locus_tag="AC16YU_04955"
     CDS             16020..16772
                     /locus_tag="AC16YU_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6064605.1"
                     /translation="MTAPANLKRSAEGAVTRWVVAGALAGVVVLGLGVASRIAVRGVV
                     VHGAAQADVDAIVELARVPVDSSLFSVSASMVADRVRRHPWVADARVSRLPTGTVSIR
                     VTERVPVLLALSSGGEPAYYVDAAGFRMPFRPGGPWRVPILTGLRERYHPVARIRNAA
                     VRDLAAALPALDDEVDALLSEFHVDRDGGLLLSTAVTPSGRTLTIRLGAAPGKSLSRL
                     RPFWDQVVLTRPDVRFTWVDLRFDSQIVTRES"
     gene            16819..18087
                     /gene="ftsA"
                     /locus_tag="AC16YU_04960"
     CDS             16819..18087
                     /gene="ftsA"
                     /locus_tag="AC16YU_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6064606.1"
                     /translation="MNERIVVGLDIGTTKVCAVVATADELDRINVLGVGVAESEGLNR
                     GVVVNIDKTVAAVRHAVGEAERMAGVSVRSVLVGIAGDHVQSFQSRGVVTISNRDGEI
                     TERDVNRLLEDTTHVAMPADREILHVIPQEFIVDGQDGVADPVGMSGVRLEANVHIIT
                     GLVSAAKNIYRCIEKAGYQVADIVLEPLGSSFAVLHPDEKEVGVALVDIGGGTTDIAV
                     FEDNTIRHTAVIAVAGNKVTDDIRKGLGIMRDQAERLKCTYGVALSERVSDDEEIAIP
                     GIGGRTEKRIGRSTLAQIIQPRMEEIIEIAAIEIKRSGYGRHLSAGVVLTGGGSLIPG
                     TDELASEILGLETRIGVPMGLSGGLVKEVDDPKFATAVGLVLYGLRPESLGGTPFRTD
                     MGSVIHEAVPAGDGLVGKIATRMKTWFDEL"
     gene            18157..19419
                     /gene="ftsZ"
                     /locus_tag="AC16YU_04965"
     CDS             18157..19419
                     /gene="ftsZ"
                     /locus_tag="AC16YU_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6064607.1"
                     /translation="MNHSFSSHFAFDDAMNEEAKICVVGVGGGGGNAVNNMIRRGIQG
                     VEFYAVNTDQQALAANLAANKIQAGAGLTKGLGAGARPAVGAEAVEESRDEIKKALKG
                     FDMVFITAGMGGGTGTGGAPIVAAIAKELGVLSVAIVTRPFECEGRRRQQSADTGIDL
                     LRAHVDTLILIPNERLLDIDDGNTSLLEAFAKADEVLYNATRGISDLITVHGLINLDF
                     ADVKTTMRNGGTALMGSATASGEKRAENAATEALSSPLLDGLSIHGARNVLVNITANQ
                     SLGIREATAATKIIQMEAGDDVEVIFGTVIDEEMGDELRITVIATGFDSHGRGAQKKG
                     QGNLALRLDPVEYKGEQALSHLDEPAYERRGRSVSTLPPSHVNGDGAPAGGTARVRRV
                     QKDEMEDRTERIAKGNDDVPAFLRKMLD"
     gene            19645..21276
                     /locus_tag="AC16YU_04970"
     CDS             19645..21276
                     /locus_tag="AC16YU_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064608.1"
                     /translation="MRTFISWAFVLVALVAQASAQRAPRLGVVWDPPADALQAEADLR
                     AIRSMGFEAVRTGLIDRASLLELADTLGIHVYQEIPLSFSTASELTDTLGVVRRLVAD
                     ALVAAEDHPSARHLGFAFAPSTIQEDVCAILDELADFARRRAAPEVRYYYVSWLPHAD
                     RCGEAVDLVLLDDPIHDRPLRLLERWTGSGPVGLAAVGRRVDPEADRGLLVEGSEERQ
                     ALYFEETIPRLLDRSDLSVAFLYRWQDPDPYDSGAFHPAPFGAVYGLQDETGRARMAR
                     DVAEGLLARGQTTFRLLAGTAPRPGASWFVLLGWLVLATLSVMVAASPRFRAMIPRYF
                     FAHGFYRHAIQEGRDVLTVISTVMLVLLGLTIGLLGAQVTRLLTDSAPLHALWYRAGA
                     EGRSAMDALLTDEALLVVAIGSAGLLVLVVWVFVWTLVRRGAALKPSQALATAVWPRW
                     PMMILLVAAMVVDGLDEEAFRRSIEWMPAAWFAVALWGTIRTNLDLAQIARVNPAATV
                     VLWLVSPVVVLLGGAVAFMLLRPELAALLWALWSA"
     gene            complement(21277..22263)
                     /locus_tag="AC16YU_04975"
     CDS             complement(21277..22263)
                     /locus_tag="AC16YU_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064609.1"
                     /translation="MSEGYVLHAYGPARYLHQAVASAVTIRRHDSRRPIALYASELHL
                     DLLRSAGIDGLFGVLEVLPEENRSIVGFKHHLDRFMPFDRCLFADADMVWCRDPDPLW
                     LQLSGFGFTATGHQKADFWFGGPKGIGVVADYLLDRRRRTMRRFGLTHLPRVQAGMIY
                     ASDRALAREACALARDFLDRRKETHFRSRLSEGRTEESCEWSLAMAMSRLDLPVFPWF
                     QGQTSPQLDFVPGLVEHDPDFERVTCRYYTDRRVQEMRGMASEGARRAITAFLTRMPG
                     RGDTMQVTPFALHFGWLRYKQPYVDFAGRTWTGLVDGIAADAEPAESLGVGN"
     gene            complement(22327..22938)
                     /locus_tag="AC16YU_04980"
     CDS             complement(22327..22938)
                     /locus_tag="AC16YU_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tgt2/MlaC family protein"
                     /protein_id="HIR6064610.1"
                     /translation="MRRILSSILHTVVLAAFLAATPAAAQNQQSEIRTMLESRDAEIK
                     RLLGRADSEVSPERRERLRDVINSVIDFEAMSRGALGPHWNDLSAAQRTEFVSVFGSI
                     IRGQSLSNLDPYRQKVTYDAIEVDGSTARVVTSTTVKDALVTVAYDMARGEGGWHVTD
                     VSIDDVSTVEGYARSFQSMIRKRGFDVLMQRLREKLVEVEAEG"
     gene            complement(22988..24385)
                     /locus_tag="AC16YU_04985"
     CDS             complement(22988..24385)
                     /locus_tag="AC16YU_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6064611.1"
                     /translation="MMRKTLCLVALLVTTTAQARQPVPDTFRVTVSAAIDRAVAVSPD
                     IRSSEAAVGYAGARLDLARASRFLTEFRATSAHSVAPGLDNPNDTPTDRLFLDPDVRN
                     DWEDLRPFNRLEVEAIQPLFTWGEIGRSIDAARHGLEAEVQAVRSDRIQVALRTGELY
                     YGLVLARSLERLTDEASKAVRDAKGEIERLLQEGDPDVDDADRFQVLITEQEFGRRVV
                     EIRERLATVESGLRRQLMLPDGVVPLPGDASMEPVPFVPDSLDRYFEIALAERPEPAR
                     ADAGVAARRALVGVARSAFYPKLFLGVEARYSSAEGRYRQYNPYLGDPFLSRTVRAGV
                     GLRLDLNFLQTRAKVEQARAQLDEVVHQSEAARQLVLYEVESAWRTLKARQAAVSAAE
                     EALHLSKEWLRTEQVNFDYDLGDTANLVSAVRTNLELQAARHQAVFDYNVAVLRLLAA
                     AGLLDRVDAFGMLLE"
     gene            24529..26046
                     /locus_tag="AC16YU_04990"
     CDS             24529..26046
                     /locus_tag="AC16YU_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HIR6064612.1"
                     /translation="MDPEAPWKEVRTLGELRASGYRVRSVKDEMRSNLIRRLREGGPV
                     FPGIVGFDETVVPQIQNAILGRHDFILLGLRGQAKSRLLRMLPTLLDDYIPVIEGSDL
                     NDDPFAPISKYGRDLVAARGDDTRVEWLHRSRRYGEKLATPDTSIADLIGDIDPVKAA
                     HHRLTFADEEVIHFGIIPRTNRGIFAINELPDLQPRIQVGLLNIMEEQDIQIRGFNIR
                     FPLDVLMVFSANPEDYTNRGSIITPLKDRIDSQIITHYPRTIETGIEITRLEAWVERG
                     DRMTVRVPHVFREIVEQIAFEARASEYVDQSSGVSARMTRAALESLVSAAERRAFLPG
                     ARETTVRPSDLWFVQPAVTGKVELVYEGEQEGAQAVARALVGRAVKRIAARYFPDPED
                     KKRGRPAYAAVLGWFSSGGSVPLHHDLDDATYRSRLEEVPGLADLARDFAEEERAVAM
                     ELALEALHQHSLLGKESMREGPTFTDMMGSMLSSLGAPEDDAWDGAFDDSPDDRP"
     gene            26043..28112
                     /locus_tag="AC16YU_04995"
     CDS             26043..28112
                     /locus_tag="AC16YU_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR6064613.1"
                     /translation="MRRPTVWRARLALAALAGLPLPALAGQQRDPRPPDHDAVLQCAA
                     SRAGYLPATSDADKRAARAGSGFDAEYYRIALDLTSPATGFVEGATRIVGRATAPVLA
                     TLELDFHVSMLVDSVVSAGGTGLSWSSLGNILAVDLEPAATAGERVDVTVHYRGWPAS
                     TGFGSFAAGTRGAGDPYVWTLSEPYGARTWWPGVDHPSDKADSVDIVVRVPDPMQVAS
                     NGLLAGTEPHGDGTTTWTWEHRYPVSSYLVSVAAGVYDHYLQEYVRPDSLAALFGEAS
                     FPIEHFAYPGSLAFEGTHPQTGWRRVVDIMPVLEWWYGPYPFQDEKYGHAHFTWGGGM
                     EHQTITSMGGNSVGLVAHELAHQWYGDAVGPATWPHLWLNEGFATMGELIVWGAMSDV
                     YPGVYETIFNLYYDRALRAQGTLVLEDTSSVSDMFAASRVYSKGGMVLHMLRSMTGDE
                     VFRDVLRAWAMHPEFRYSVASTSDFRRVAEEVTGLDLGRFFEQWVTSGTGHPEYRATW
                     TSARDRDRWIARVTLDQVQDATESGVFVFEMPVVIRIVTDSGEHDYTVLNDERSQVVH
                     VDLGDEPREVIIDPDRRILRAHEAPVTFEGETPPEFLPSARLVSIHPNPSDGVLRVRI
                     DAVGGRSGTIEVTDAVGRRVRTLDVTLAPGRQTIDLDVGDLAAGVYVVRLVAGEARDS
                     RTVVVIR"
     gene            complement(28125..28373)
                     /locus_tag="AC16YU_05000"
     CDS             complement(28125..28373)
                     /locus_tag="AC16YU_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064614.1"
                     /translation="MSKRTRTYLLILIGIFLAFVMMDSQGVFDSKSWIEIPHGSHSHY
                     LPKNYAECNPPLTVGDGPTRAPGPGETIDCSGNIVPER"
     gene            28521..>30781
                     /locus_tag="AC16YU_05005"
     CDS             28521..>30781
                     /locus_tag="AC16YU_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6064615.1"
                     /translation="MIRRAAILLVLLASTGAARDASGQLATVRGRVVEAETGAALPGA
                     TVQLTDSNGAERGVSAGPDGRFEMRRLPPGTYVLGVSFVGFEPRVDTLQVGFGATLNV
                     TVRLETSQKQLEEIVVEEDRREARVGAGASSIQPAALARVPMPDVTYDLAGYLLTLPG
                     FVSTGDRGGQLFVRGGTPTQNLVLIDGIPIFQPFHIVGFYSAFPADIVSWADVYAGGF
                     TARYGGRISAVVDVTTRNGDKRRLRAAGSIAPFLASVRAEVPVARDQVSLVASWRESV
                     IDRIAPDLLGRGLPFRFGDRFAKLHAYLSPTSSFSFTILNTFDEGALDVSGSEGSTAE
                     ESPAEWGNTAFGSRYTYMPSEAPVVAQFSIYYTRLVSRYRPLARRPQESDIRAVTMAV
                     DFVYLLGSVRLQSGIFGNLHQFVYDLGNRFGGVRTGVTSGGVFVDGRFDADSRLRIEP
                     GLRMETFSQGISTALDPRLRVTLLTLGGRQQWTLAYGRYRQQIVGVNNEQDVSDVFSV
                     WAPSPRATAVPQAVHYLAGVNSRVLPWLELSLDGYRKDFSNLTFPVFNREPQHSGSFS
                     LVEGDAMGVDVRAEVNRPSFHFSTSYSLADITYRWSGLSRGGHTIEGLTTVPAPDDRP
                     FDPPHDRTHQVNAMAQVSRGPWRVSVRWQYGTGLPYTRINGFYDGVRVTPDPAQPHLD
                     EPGELLVSRGATLDSRFPAYHRLDLSVERDFRFRVATATLQAGLMNAYDRANLFEYNY
                     VTGERIDQLPLVPS"
BASE COUNT         4623 a        10458 c        10930 g         4770 t
ORIGIN      
        1 gcggcggcgg attcatgttc ttcttcaccg ccttcgaccg gcgcagggcc gtggaggagg
       61 ccctccgcaa gtccggggcc acggtggtac atttcggctt ccgagacggc gggatcgaga
      121 catggacgcg atgaccaggg ccgaggccgg gaaatgggtg acggcgcaga tcgacgaggc
      181 cgcaacggtc caggaggccg tgcgctcgat ggccggccag ctggtagaga tcgctttcgc
      241 catttccgac aggctttcgg cgggagggca ggtgctgttc ttcggtaacg ggggaagcgc
      301 ggccgacgcc cagcactggg cagccgagct gtccggaggg ttctaccgcc gccgcccggc
      361 gctgcccgcc gccgctctca ccgccaattc ctcgcaggtg acggcgatcg ccaacgacct
      421 cggattcgag gaggtgtttg cgcgtccggt ccatgccctg ggacgcccgg gcgacgtggc
      481 cgtcggcatc tcgacgagcg gccggtctcc gaacgtgatc gtggcgctgg aggcggccag
      541 gagccggggc atgatgaccg tcggcttcac cggccgcgac ggcggtgcca tggccccgat
      601 ctgcgacctg ctcatcagca tcccgtcgga cgacgtggcg cggatccagg aggggcacga
      661 actctgtgcg cacgcggtgt gcgccgtcat cgaacacctg cagtttccgg atgccgagcg
      721 tggttgaccg gagagccgcc gtattcctgg atcgggacgg cacgctgaac gaggaagtcg
      781 agtacctgca ccggccggag gatttccggt ggatacgcgg tgctccggaa tccatccagg
      841 ccctgaaccg ggccggcctg ctcgtgatcg tgatcacgaa ccaggcgggt gtcgcgcacg
      901 gccgctacga cgaggcggcc gttgcgcacc tgcacgaatc catgcaggcg gacctggcgc
      961 atcgcggggc acggatcgac gcgttctaca cgtgcttcac gcaccctgac gcacggatcg
     1021 agaagtacag gcgtgacgat ccctgtcgca agcccggcaa gggcatgttc ctgcacgcca
     1081 tcgacgattg gtcggtcgac gcgggacggt ccttcatggt gggggacaag aatacggacc
     1141 tcgtgcccgc ccgggagctc ggcatgcgga ccatcctggt gagaaccggg tacggggcgt
     1201 cgatggaatc aaccacggtc gcggaccttg tcgagtccga catcgccgcc gccgtcaagc
     1261 acatcctggg gcaatcgact acgacccgat aaaggaccgg atcggggccg ccgtggagcg
     1321 gcaccccctg ctgcagcgga tattcttctc gctgctccac gtcgttttcc tcaggagctg
     1381 gtacgtgcgg agggcactcc gtgacgccgt gccccgggac ggatcgccga tccggctgct
     1441 cgacgcgggg accggtttcg gacagtatgc ctggcacgtg ctccgcaggt atccgcatgt
     1501 ccgcgtccac gcggtggacg tcaaggccga gtatctcgaa aacctcggga ggttcgttgc
     1561 gtcgaccggc cgcgcggacc ggctcacgct cgcggtcgac gacctgaccc gcatggaatc
     1621 ggaggggccg ttcgacgtca tcctctcggt ggacgtcatg gagcatatcg aggatgaccg
     1681 cgcggtattc cggaatttca gggagatcgc agcaccggga ggccgcgtgc tcatcaacac
     1741 cccgtcggac cagggcggat caggggtcac cgccgaggga ggcgcgtcgt tcataggcga
     1801 gcatgtgagg gacggttacg cggccgacga gctctgcatg aagctgcggg atgcggggct
     1861 ggagatcgaa tcggtgacct acacctacgg atcggcggga agcctggcct ggcgaatgct
     1921 gctccaggtg cccatgctcc tgttggcgaa gggattctgg acgcttcctc tcgtggcgct
     1981 ctggtatctg ccggcctttc cgatgggcat gattctcaac gccgtggacg ttttgcgcga
     2041 caatcggacg ggcacgggtc tgctcgtcgt ggcgcggcgc ccgtagtccg tggcggggac
     2101 gtttcgaccc tcccggggat tccgtatttt ccgcgggcat ctcaggccga cggtgcccct
     2161 ccgggccgca ccgccagggc cgacccggag ccagaccggc cgcatgtgca ccaccttccg
     2221 ggtaccgtcg tgcagaccaa gtacatcttc gtgaccggcg gcgtcacgtc ctctctgggc
     2281 aagggcatcg tctgcgcatc cctgggccgg ctgctcgagg cgcgtgggct ccgcgtgacc
     2341 atccagaagt tcgacccgta catcaacgtc gatcccggga ccatgaaccc gtacgagcac
     2401 ggcgaggtgt tcgtgacgga cgacggggcg gagacggacc tggacctggg gcattacgag
     2461 cggttcctgg gcgtcccgac gagccaggcg aacaacgtca ccacgggtcg catctacctc
     2521 gaggtgatca cgaaggagcg ggcgggcgcg tacctgggca agaccgtgca ggtggtgccc
     2581 cacatcatcg acgagatcaa gcactggctg atgaagctgg gtggaaccgg cgaatacgac
     2641 gtggtgatca cggagatcgg aggcacggtg ggcgacatcg agggtcagcc atacctggag
     2701 gcgctccgcc aggtccgcta cgagctgggc tcgcgcaacg tgctgaacat ccatctcacc
     2761 ctggtaccgt acctggaggc ggcgggcgag gtgaagacca agcccacgca gcactccgtg
     2821 aagacgctgc tctccttcgg ccttcagccc gacgtgctgg tctgccgcac ggaccggccg
     2881 ctcgacctgg acgtccgccg gaagctggcg ctgttctgca acgtggagcc gaaggcggtc
     2941 atcgcctcgc gcgacgcgga atccatctac gaggtgccgc tgctcctgaa ggaggagggg
     3001 ctcgacgaga tcgtcatgga tcgcctgcgt ctcgccgagg atgccgagcc cggacgggtc
     3061 acggatccgg acctgtccga ctggatcggc ttcctgaagc gcctgaagga gccgggagcc
     3121 accgtgcgcg tggcgctcgt cggcaagtat gtcacgcacc aggacgccta caagtcgatc
     3181 aacgagtcgt tcatcctggc cggcagccgg cacggggtgg ccgtggaggt gaagcaggtc
     3241 ctgtcggatt cgcttacgga agagaacgtg gcggaggtgc tcggcgactg ctcgggcatc
     3301 gtcgtggcgc ctggattcgg ggaccggggc atcgacggca agctggtcgc cgtgcggttc
     3361 gcgcgcgaga acgacgtgcc gttcttcgga atctgcctgg gcatgcagtg cgccgtggtc
     3421 gagttcgcgc gcaacgtgtg cggatgggac cgggcccact cgaccgagtt cgacgccgac
     3481 acgccgtacc cggtgatcga cctgatgaac gagcagaaga tgatcgcgga caagggcggg
     3541 acgatgcggc tcggcgccta cgactgctcg ctcgccgagg gctcgctcgc gcgcggcata
     3601 tacggacagg ccgaggtccg ggagcgccac cgccaccggt acgagctcaa caacgtgctc
     3661 cggtacaagc tcgccgagca cggcatgtat ttcacgggca tcaaccggag ccgcgacctc
     3721 gtggagatcg tggagctgcc ggaccggcgt tggttcgtgg gcgtgcagtt ccacccggaa
     3781 tacaagagca cggtgggccg gccgcatccg ctgttcctct ccttcgtgga ggcggtcatc
     3841 ggacacgccc gcgaccagga cctgatcaag agtccgaagc ccgtccggcg ggagaagaag
     3901 ctccagctcg cctcggccaa ggtctgaccc gccggtccac gccggctccc cccgttcccc
     3961 cacctcgggc cgccgggcgg ccgcggggca gacgtgcgtc cggccgcggc ggactcctcg
     4021 gcgtgccgtc ccctcgggga ttccatcggc ttgccccccg aatatccacc gtgtcttgat
     4081 tttacatgtg gatcacttac attccgggga cgagtgggga attgtgggga attctcccag
     4141 tcaccgcaaa tacctgcgga cgcagtccac cctgcatcca cagccatggc cagcttcaag
     4201 ggacaggcag attactcggt cgacgggaag ggtcgcgtgg cgattcccgc caagatgcgc
     4261 gcggccctga gccccgaggc caagggcacg ttcaccatga cccgcggttt cgagcgctgc
     4321 atcgtcctgt acccgctcga cgagtgggaa cggatcgagg cggacatcgc gaacctgaac
     4381 atgtacaacc gccagagccg ggacttcgcc cgcatcatcc tgatgtgggc ggacgaggtg
     4441 gcgctcgacg cgcagggccg cgtccccctg acccggccgc tgatggactt cgcgggcatc
     4501 cgcgagcggg ccctgatcat cggcgcgctg gatcacgtcg aaatctgggc tccggacgcg
     4561 ttcgcgacat acgagaagga gcaatccgca gactacgaaa ccctcgcaga gcgtgtgatg
     4621 ggtgggtagg atgccgccct ccgacgagct tgacacatcg gtacccgggg cggagggaga
     4681 cgatcccttc gcgacgggtt accacgcccc tgttctttgc atggctgtcg taagcggact
     4741 gatcctgagg agggacggtc tctacgtgga tgcgacgctc ggcggtggcg gccatacggc
     4801 tgcgctgctc gaggcgctcg gcccgggcgg gcgcgtcatc gccatcgacc gcgacgggga
     4861 cgcgatccgt gaggccggcc gacgcctgga gccggaggtc ggcaagggca ggctgcgcct
     4921 ggtgcgcggc aacttctcgg agctcgagac gatcctggcc ggattcggcg agagccgggt
     4981 cgatggcgtg ctgctcgacc ttggcgtgtc gtctcaccaa ctggacctcg gatcccgcgg
     5041 attcagcttc cggatcgacg gacccctgga catgcgcatg gatgaccgtg ccggggcgac
     5101 cgcggccgac ctggtcaacg ggctggacga ggcggggctc cggcggctcc tgcggattta
     5161 cggcgaggag ccgtcggccg cccgcatcgc ccgcgccgtg gtcgccgcgc gaccggtcct
     5221 cacgacgacg gggctggccg aggcggtgcg cggggcggtc cccggcgcga gaaactcggc
     5281 caaggcgctc gcgcgggtct tccaggccct gcggatcgcg gtcaacgcgg agctcgacgc
     5341 gctcgaggac gcgatccggg cggccaccag gcaggtgcgg gtcggcggcc ggctggccgt
     5401 catcagctac cactccctgg aggaccggcg cgtgaagaga ttcatcagat cagggaattt
     5461 cgagggcagg ctcgagcgcg acctgtacgg caatccggtc gtgccctggc gggaagtgca
     5521 tcgtcacgcc ctgaagccgg acgaggagga aatcgtgcgc aacccccgtg cacggagcgc
     5581 ccgcttgcgc atcgccgaac gaatcagcga ggaccgaccc acggggtcgg tatgacggta
     5641 tggccaggtc cagaaccgga aagagcaccc gggccgtgcg gttcgtaaag aacccaaggc
     5701 ctgtcgagcg gcggcgatca accgccgccc gcgtccgcgc gcagcccatg cgcgcggcgg
     5761 agcccgtcgt accgagccgc cctcgtccgt cgacgaacgg aatgccgggg tggggcgagc
     5821 tcgagaaggg caaccgccgg gagggcgtgc gcaagggggg atgggatgtg cttctggaca
     5881 cggtgcccac gattcgactg tccctctaca tcctgggcgt ggcgatcctc gtcaccctgt
     5941 acgtcggtca cgtccactcc acgcaacgcc tcgcggagcg ggtgcagaac atgagccgga
     6001 cgaacctctc gctccacctt cgccacaacc gcgtgaaggg tgccttcgac cgggcttccg
     6061 gtccggcggt gatctacgag cgcgcgagac ggctcggact ctcggagtcg cttccgaacg
     6121 gctataccgt aatcaccgac tgatcccgga acgtgagctc ccggcccctg accagccgcc
     6181 ccccgtatgc acgtgaagga cgaaatactg gcgaggatgt acgtgctgct gacgctgctg
     6241 gcggtggtgc cgctcctggt cgccgtccag gtggtgcgca tcaccctgat ccagggtgcg
     6301 gagctccgcg agcagggcga gaggcaggcg accagccagt tcacgatccc cgcgatgcgc
     6361 ggcacgatcg tggaccggca cggacggacg ctcgccgtga acacggcccg atacgatatc
     6421 gccgtggacc ccacgattcc gggataccgg gaccgcgcgc ccgcgttctt tgacaggctg
     6481 gcacggacga cgggccgctc gcccgcatgg taccgcacgc tcgtggcccg gagctcgagc
     6541 cgacagtacg cccggctgga gcgtggaata gacgagtcgg cgctggacgc gctgcgcgag
     6601 atcgatctcc cgggcctcat cgtgcagtcg cggttcagca ggcggtacac ctacggcacg
     6661 gccgcggcgc acgtgctcgg ccacgtcgac acggacctgc acggcatcgc gggcatcgaa
     6721 tcacggtacg agcccgtgct ggcgggcatc gacggcaggc agtccgcgca gcgggatcgc
     6781 cgcggggtgg cgagggcgtc ggccgacggc atgaccgtcg aaccggtgca cggggaaacc
     6841 gtggtcctga cgatcgacct ggttcgtcag gcggtcctcg aggaggagct cgcccgcggc
     6901 gtggcggcca cgggagcgaa atggggcagc gccatcgcca tggatccgtg gacgggcgcc
     6961 gtgctcgcga tggcgaacgt ccccacgtac gacgccaacg atccgggggc gtactcgcac
     7021 cccgagatgc gcaaccacgc ggtcacggac cagctcgagc ccggatcgac cttcaagctc
     7081 gtgaccgcgg tggcggccat cgaggaggat gccgcgtcgc tcgacgacag cctcgagacc
     7141 ggggacggat ggatcgtgct ccacggacga acgctccgcg acacgcacgc gctcggcacg
     7201 gtcaccttcc gcgacgtcat cgcgcactcg agcaacgtcg gcatcgcgct cgtggcggaa
     7261 aggctgaagg ccgccgactt ccaccggcac gccagctcgc tgggcttcgg gaggaagaca
     7321 gggatcgacc ttccgggcga ggtggccggc gtgctgaagg atccgaagaa ctggtccggc
     7381 accacgctca cgtccatgag ccgcgggtac gaggttgcgg ccacgcctct ccaggtgctc
     7441 gtggcgtacg cggcgctcgc caacggcgga acgctggttc ggccctacgt ggtcgccgag
     7501 cggcgcgacg tgacggggaa cgtggtgtgg accgcatcgc aggactcgct gcgccgtgcg
     7561 ttccggaggt cgaccgcgcg cacgctgatt ccggcgttcg agcaggtcgt gaccgacggc
     7621 tccgccaggg aggcggccgt tccgggcgtc cgcatcgcgg gaaagaccgg tacggcccgg
     7681 aaggtgcggg acggctcgta cgagtcgggc gcctaccggg cctccttcgt gggcttctac
     7741 cccgtggagg acccgcgcgt ggtcctcatc gtcgtgctcg acgagcccag gacctcctat
     7801 tacggcggat ccgccgccgc gcccgtgttc cgagaaaccg ttcgccggtg gctcgccacg
     7861 acggacgacg ccccggtcgg cacgctcgcc gaggcacccg acccggacga catcgagatt
     7921 ccctcgccgg acgtcgtccg gcttccgctc gcgctggccg cagaccgggt ccgggcgtac
     7981 ggactccgga cctcggtctc ggacgacggg ccccgcctca ccgtgacctc ccagcgcccc
     8041 gagccgggcg cggcgctccg ccagggttcg cgcgtgcggc tggcggccga attcggagac
     8101 gcgaccgagg cgatgccgga cctccggggc ctgacggtcc gcgaggccgt ctggtggctc
     8161 acctccctcg ggatcacgcc gcgcatcgag ggctccggca ccgtccggag ccagtcaccg
     8221 aacccgggct ctccgctcgg tcagacggcc gtgctgcgct gccgctgatg ccatgaccgg
     8281 acgatccgtg cccatcactt tttccccgca acccctggcg accctggtcg accgccttcg
     8341 tcgggccggc ctcgtgctga gcgccgagga cgcgggggac gggatcgtcg accacctggc
     8401 gttcgacagc cgggaggtcg ggccgggcgg gctgttcgtc gcgatccgcg gagagcacac
     8461 cgacggccac ctgttcattg acagggctgt ccagaacgga gccgccgcca ttgcatgcga
     8521 ggcggcgccg gcgggcgact ggccggccgg cgtcgcccgc gtgcgggtga gcgactcgcc
     8581 gcgggcactg gccgagctgg catgcgccgc gttcggggac ccgagccgcg cgctccgcac
     8641 gatcggggtg acgggcacga acgggaagac gaccacggcc tggctctgcg cgcagggact
     8701 ggacggggtg ggcctgccgt gcggctacct gggcacgctc ggaagcggcc gcgtgcagcg
     8761 catgagcccg ggcacacaca cgactccgga cgcggtgtcg ctgcaggcct tcctgcgcgg
     8821 cctcgccgac gaggcctgcg cggccgcggc gctcgaggtg tcgtcccatg ccctggtcca
     8881 gcggcgtacc catggcatcc ggttcgaggt gggcgtcttt acgaacctga cgcgggacca
     8941 tctcgactac cacaccgact tcgagtcgta cgcgtcggcc aagcgtctcc tgttccagtc
     9001 catggccgcc gactcgacgg ccgtcgtgaa tgcggacgac ccggcggcgg cgtccgtcgt
     9061 gggcggaacg ccgcctcgcc aggtgcgcta cggcacggga acggatgccg atgtccggta
     9121 cgcggtacgg ggtgacggca tcggcgggct gcgtttgcac ctggacgggc acgaccgccg
     9181 gttccggctg tcgggggcct tcaacgcgtc caacctggcg gcggcctacg cgacgctcgt
     9241 tgcgctggga gtggacgggt ccgaggcggc ggaaagcctc tccgaggcct ctcccgcccc
     9301 cggccggttc gagccggtcg ccgctccggg gtgcgcggac ttcctggtgg actatgccca
     9361 cacgcccgac gcgctggccc acgcgctcgc cgcggcccgg aagctcgtgg ggcgtgacgg
     9421 gcggctgtgg tgcgttttcg gctgcggcgg cgaccgtgac gccggaaagc gtccccagat
     9481 gggagccgtg gccgaggccg gggcggaccg ggtggtcgtg acgagcgaca acccgcggac
     9541 ggaggacccg gagcgcatcc tcgaggacgt gcgctcgggc gtgtcccggc cggaagacat
     9601 gctgtggatc gtggaccgtc gcgaggccat ccacgccgtc gcgaggctcg cggatgagtc
     9661 ggacgtcgtc gtcgtggccg gcaagggcca cgagacctac caggtcgttg gcgaggagcg
     9721 cctgcccttc gacgaccggg acgtgatcct ggaagcctgc aaccaggtgg cctgacgcat
     9781 gctctactac ctgatactct ggctcgaacg ggaattccag ccgcccggct tccaggcctt
     9841 ccagttcatt acggtccggg cctcgctctc cgccatcacg gcgctcttca tcgcgctgat
     9901 catcgggcgg cggatcatcc gctggctcga gcggcagcag atcggggagc aggtccgcca
     9961 gggggtctcg gcgggggccg tggaccactc gcacaaggcg ggcaccccca cgatgggcgg
    10021 actcatcatt ctcttcgcgg tcctgggcgc gaccctcctg tggggcgcga tggccgaggt
    10081 ctacgtctgg ctcatggtcg tcgccacggc ctggatgggc gccttcggat tcgccgacga
    10141 ctacatcaag gtggtgaagc gggacaagag cggtctcccg gcacggatca agctcatcgg
    10201 ccaggtctcg ctcggcgtgt tcgtgggctg cgtgatctac ttccacccgg ccttccgcga
    10261 cgtccacacg ctcacctacc tgccgttcgt ggccgaccag acgctggact acaacgtgtt
    10321 cggaagctgg ttcggcggcg tggacctggg ctgggtgatc tacatcccgg tggtggtgtt
    10381 catcatcacg gcgctctcga acgccgtgaa cctgacggac gggctcgacg gcctggccgc
    10441 cggcgtcacg ggcatcgtcg cgctgggtct cacggcactg gcctacatcg ccggcaacgc
    10501 cgtcttcgcc aacttcctga acgagatgct actgcccggc gcgggcgagc tgaccgtgtt
    10561 cgcgtctgcg ctcgccgcgt cgtgcttcgg attcctctgg tacaacggct atcccgcgca
    10621 ggtcttcatg ggggacaccg gggccctgtc cctgggcgcc gcgatcggta cgctgacgct
    10681 gctcgtccgg aaggagctcc tcctgccgct cctcgcggcc gtgttcttca tcgaagccgt
    10741 ctcggtgatc atccagacca cctggttccg ccatacgcga aggaagacgg gaacgggcag
    10801 aagggtcttc cggatggccc ccctgcatca ccatttcgag gtcggaggca tgcacgaggc
    10861 gaagatcgtc gtgcgcttct ggctgatcac cgccttcacc gtcatcgcga cgctcctcct
    10921 gctgcgcatc cgataggaat gatcgccccc ctctccgaga tgatatcgcc caagctgcgc
    10981 cacgagtccg tgagcgtcgt gggcgccgcg cgctcgggcg tcgcggtgtc cctgctgctg
    11041 gcggggtacg gggcgcgcgt gttcgtcacc gaccgggccg acatcccgcg gacgacgctg
    11101 gatctcctgg cccggcacgg ggtgaaggcg gagtcgggcg gccactcgga ccgggcgctc
    11161 ggagcggacc tgctggtcgt gagcccgggc gtgccgacga ccgcgcccct ggtcatggag
    11221 gccatggagc gcgggattcc cgtccggtcg gagctcgagg tcgcctcgtg gttctgcgag
    11281 gcccccatcg tggcggtcac gggatcgaac ggcaaaacca cgaccaccgc gctcgtcggc
    11341 cacgtgttcc gcacggccgg ccgcaccacg tgggtggccg gcaacatcgg cctgcccttc
    11401 tcgtcggtcg cgtccagggc ggggcaggac gacgtcgtgg tcctcgaggt gtccagcttc
    11461 cagctcgacc acacggagag cttccggccg cgcgtctccg tgctgctgaa catcaccccg
    11521 gatcacctgg accgctacgg tcacgatctg gaggcgtacg cggcggcgaa gcttcgcatc
    11581 gccgagcgcc agggggcggg tgacgccgtg gtctacaacg cggacgatcc gattctccgc
    11641 cgcttcgccg agtcgctcga tgccggctcc gggccgacgc ccctcggcgt cacgctcggg
    11701 acgccggacg cccggtccgc cgcctggctg accgaagacc gtctcaccct cagaatccaa
    11761 gacacccagg aggacttcat gcgggcagaa gagttggctc tcaggggccg acataatctg
    11821 tacaactcac tggcggcggc ggtcgcggcg cgcgtgatgg aagtgcgcag cgacgtggtg
    11881 agggcgagcc tcatgtcgtt cgagggcatt ccccatcgcc tggagacggt gcgcgagctc
    11941 gacggcgtca agtacgtgaa cgactcgaaa gcgaccaacg tgaacgccgt ctggtacgcg
    12001 ctggagagtt ttcgcgagcc cgtgctcctc attgccggcg ggcgtgacaa gggcaacgac
    12061 tacgagccgc tgaaacgggt ggtgcaggac cgggtccgcg tgctgctggc gatcggggag
    12121 agcgccgacc acgtggtccg cgagctgggg ccccacgccg gggagtgctt ccgggccggc
    12181 accctggacg atgcggtgca gatggcccgc accctggccc gcgctggcga cgtcgtcctg
    12241 ctgagcccgg cctgtgcaag cttcgaccag ttcgacaact tcgaggatcg tggcgacacg
    12301 ttccgccgac tcgtagcaga cctgtagacc catggccacc gcagcgacat cgcgactgaa
    12361 gatccggccg gccgacaagt atgtcgtctg gatcgtgctg atcctggcgg ccgtcggggt
    12421 ggtggcggtc tacagcgcga tctcgtttct cgcgcagacc cgggcgggtg gggacaccga
    12481 gcagttcctg gtgcgccacc tgctccgcct cggcctcgcc ttcggggtca tggtggcggt
    12541 gagcctcgtg gactaccgga agctggcccg gatcgcgagg ctcgcgctcc ttggatcctt
    12601 cggcctgctc ctcctggtgc aggtcgtcgg gaccacgtcg ggcggagcgg cgaggtggct
    12661 gcagttcggg tcgttcgggt ttcagccgtc ggacatcgca cgcgtgtcgc tcgtgctgta
    12721 cgtcggcacg ctgctggccg ccaagcaggc ctacatcaag gaattcggcc gcggcttcgc
    12781 gcccatcctg gtctggatcc tggcgtcggt catgctcatc gggctcgagg acctctcgac
    12841 cgcagccgtc gtgctcgccg ccgtcggggc catgtgtttc gtgggccgtg tgcacgtcgc
    12901 ccagctcgcc ggcgtcgccc tgctcggact gctgctcgcc tacatgatgc tgctctcctc
    12961 gccgcaccgc gcggcccgca tcgaatcgta cgtgggggtc cgtctcttcc cgaataccga
    13021 gggcatcgac gtgttctcgg cccaggaaga gggcttccag gcccaccagg cccggatcgc
    13081 gttcgcgatg ggcggactga ccggtgcggg gcccgggaag agcacccagc gggacttcct
    13141 gccggcgccc tacaacgatt tcatcttcgc gatcatcgcc gaggagtacg ggctcgtcgg
    13201 gtccatgggg ctgctcgcgc tcttcgtgct gcttctcgcg cagggcttcc tgcggatcgc
    13261 gcgccactcg cccgatccgt tcggactgtt cctggcggtc ggtctcaccg tgatgctcgt
    13321 gctgtacggg ttcgtgcacg ccggcgtggc ctccggcctg ctgccggtca cgggccttcc
    13381 gctgcccttc gtgtcgtacg gcggcacgtc catggtggcg aacggcgcga tggtcggcat
    13441 cctgctcaac atctcgaggg cggtgcgctg accatgccgc gacccccgcg aatactgctg
    13501 gccggcggcg gaacgggcgg gcacgtgtat cccgcgatag ccattgccga cgccatccgg
    13561 cgaacggagc cggagtcggt cgtggcgttc gccgggacga ccgaccgcat ggagtgggac
    13621 gccgtcccga aggcggggta cgccatccat ccgatcgcgg ccgccggaat ccggcggagc
    13681 ctctcgctgc ggaacctgcg cgttcccctc acgatggccc gcggtctggc ccagagctgg
    13741 cacctggtcg gcgacttcga tgccgacgcg gtggtcggaa cgggcggcta cgtctcgggc
    13801 ccggtcgggc ttgccgccgc gatgagacgg agggcgctcg tggtgcagga gcagaatgcg
    13861 cgccccggag tcaccaatcg catcctgggg cgcatggccg accgcatcca cgtcgcgttc
    13921 gacgaggcct gggccgcctt cccgcccggc cggacgacgg tgtccggcaa cccgatccgt
    13981 cccggactcg ccgccatggg acgcgacgag gcccgcgagc gctacggcat tccagccgaa
    14041 gcgcgggtgc tcctggtgct gggcggatcg ctcggaagcc aggccatcaa cgaggccgtg
    14101 gcccggcacg tggggcggct gctggagtcg ccggacacgg tggtcatctg gcaggccggc
    14161 tcccgctacg tggagcgcat ggcggcggca gttcccgggc acgagcgcct gaccctttcc
    14221 gccttcatcg accgcatgga cctggcgtac gcggcggccg acctggtgct ctgccgctcc
    14281 ggggccatta cgtgttcgga actgctcgcg acgggcaggc cctcggtgct cgtcccgagc
    14341 cccaacgtga ccgaggatca ccagacgcgc aacgccgaga gcctggtccg gatcggggcc
    14401 gcgcggctgc tcgcggagtc ggggctcgcc gaggggtggg agcccctggt ccggcagctc
    14461 ctccacgatc ccgaaaccct cgcatccatg gccggggcgg cccgctcgca cgcggtcctg
    14521 gactcggccg accgcgtcgc ccgcgacgta ctcgacctgg cgcgccaccg cgccgcccgc
    14581 gccgcatgac acttccccgc agacaaccct cgttcggccg gatccgccgc gtccacatgg
    14641 tgggaatcgg cggcatcggc atgagctcga tcgccgaggt gctgctcacc cgcgggttcg
    14701 aggtgtcggg ttcggacctg cagcggacgg acatcacgga ccggctggcg tcgctcgggg
    14761 cccgcatctt cgagggccac gccgcggcga acgtcgaggg cgcccacgtg gtcgtccact
    14821 cctcggccgt ccatgcggag tcgaaccccg agacgatcga ggcggcgcgg cagcgcattc
    14881 cgatcatcgg ccgggccgtg atgctgggcg agctgatgcg gaccaagtac ggtatcggca
    14941 tcgccgggac acacggcaag accacgacga cgtcgatggc cggcctcgtc gtgacggagg
    15001 gcggcttcga tccgacgatc atcgtcgggg gcaaggtgtc ggtcttcggc tcgaacgcgg
    15061 tggccggcga gggcgacatc atcatcatcg aggccgacga gtacgaccgg acgttcctgc
    15121 gcctgacacc gtcgctggcc gtcatcacga acatcgagga agagcacctg gacatctacg
    15181 agggcatcga cgacctgctc gacgccttca cccgattcgc cgggagcgtt cccttcttcg
    15241 gggccgcgat ctgctgcctg gacgatccga acgtgcagcg catcgtcggc tccatcgacc
    15301 ggagggtggt gacgtacggc atctcgcgcc aggcgtcggt gcgtgcggac cgcgtgcgcc
    15361 aggacgggct ggcgatgcag ttcgacgtgc tccaggaggg ccggcgcctg gggtcggtgg
    15421 acctcgccgt gcccggcgcc cacaacgtgc tcaacgccct cgccgcggtc gccgtcggac
    15481 tggaactgga catcccgttc gagcgcatac gcgccgggct cggccggttc tcgggcgtgc
    15541 agcgccggct tcaccgcatc ggcgaggcgg gcggcatcct ggtcgtggac gactacgcgc
    15601 accatccgac cgaaatccgc gccacgctcc agacggcggc gcgctcgtgg ccggaccggc
    15661 ggatcgtggc ggtcttccag ccccacctgt attcgcgcac ccgggatttc ctggacgagt
    15721 ttgcccgggc cttcttcgac gccgacgtcg tgttcgtgac cgacgtctac gcggcccgcg
    15781 agaccgagat ggacggagtg aacgggggcc aggtggccga gcgcgcccgg gccttcgggc
    15841 accgcgccgc gcactacgaa cccgatcgcg aggcgctgcc ggcgctcgtg gcccgcatgg
    15901 cgcagccggg cgacctggtg atcacgctcg gcgccggcga catctggcga ttcagccgcg
    15961 ccctgctccg cgagctcggc gcggaaaccg gggcggaaac cagggaggag gcctgagcca
    16021 tgacggcacc ggcaaatctg aagcggtctg cggaaggcgc cgtcacgcgg tgggtcgtgg
    16081 cgggagccct cgcgggggtg gtcgtcctcg gcctgggcgt cgcgagccgg attgccgtgc
    16141 ggggcgtggt cgtgcacggc gccgcgcagg cggacgtgga cgcgatcgtc gagctggccc
    16201 gcgtgccagt ggattcgtcc ctcttctctg tatccgcctc gatggttgcc gaccgcgtcc
    16261 ggcgccaccc ctgggtggcc gacgcccgcg tgagccgtct gcccaccggc acggtttcga
    16321 tccgggtgac ggagcgggtc ccggtgctgc tcgccctctc gtcgggagga gagcccgcgt
    16381 actacgtgga cgccgcggga ttccggatgc ccttccggcc gggcggcccg tggcgcgtcc
    16441 cgatcctgac cggcctccgg gagcggtacc accccgtggc ccggatccgg aacgcggcgg
    16501 tgcgcgacct cgcggcggcg ctgcccgcgc tcgacgacga ggtggacgcg ctcctctcgg
    16561 aattccacgt ggaccgggac ggaggcctcc tgctgagtac ggccgtcacg ccgtcgggac
    16621 gcacgctcac gatccgcctg ggcgcggcgc ccgggaagag cctgtcccgg ctcaggcctt
    16681 tctgggacca ggtggtcctg acccgcccgg acgtccgctt cacgtgggtc gacctccgct
    16741 tcgacagcca gatcgtgacc agggaatcat aaccgaatgg ccgccggccg gcggcatcac
    16801 ccgaaaacca atgcagccat gaacgaacgc atcgtcgttg gactcgacat cggcacgacg
    16861 aaggtctgcg ccgtggtcgc cacggccgac gagctcgatc ggatcaacgt cctgggtgtc
    16921 ggagtcgccg aatccgaggg cctgaaccgc ggcgtcgtcg tcaacataga caagaccgtt
    16981 gccgccgtcc gccatgccgt gggcgaggcg gagcggatgg cgggcgtgtc cgtccgcagc
    17041 gtcctcgtgg gcatcgcggg cgaccacgtg cagagcttcc agagccgggg agtcgtgacc
    17101 atctcgaacc gggacggaga gatcaccgag cgggacgtga accggcttct cgaggacacc
    17161 acgcacgtcg cgatgccggc ggaccgcgag atcctgcacg tgataccgca ggagttcatc
    17221 gtggacgggc aggacggggt ggccgatccc gtgggcatga gcggcgtccg cctggaggcc
    17281 aacgtgcaca tcatcacggg cctggtgtcg gccgcgaaga acatctaccg atgcatcgag
    17341 aaggccggct accaggtggc cgacatcgtg ctcgagcccc tcggctcctc cttcgccgtc
    17401 ctgcacccgg acgagaagga ggtcggcgtc gccctggtgg acatcggcgg cgggacgacc
    17461 gacatcgccg tgttcgagga caacacgatc cggcacacgg ccgtgatcgc ggtcgccggc
    17521 aacaaggtca ccgacgacat ccgcaagggt ctcggcatca tgcgcgacca ggccgagcgc
    17581 ctgaagtgca cctatggcgt ggccctctcg gaacgggtct cggacgacga ggagatcgcg
    17641 atccccggca tcggaggccg gaccgagaag cgcatcgggc ggagcacgct cgcgcagatc
    17701 atccagcccc ggatggagga gatcatcgag atcgcggcca tcgagatcaa gcggagcggg
    17761 tacgggcgtc acctgtcggc cggcgtcgtg ctgacgggcg ggggctcgct gattccgggg
    17821 accgacgagc tcgcctccga gatcctgggc ctggaaacac gcatcggcgt cccgatgggg
    17881 ctgtccgggg gcctggtcaa ggaggtggac gatccgaagt tcgccaccgc cgtgggcctg
    17941 gtcctgtacg gactgcgacc cgagtcgctc ggcggcacgc cgttcaggac ggacatgggc
    18001 agcgtgatcc acgaggcggt gcccgccggc gacggactcg tcggcaagat cgcgacccgc
    18061 atgaagacct ggttcgacga actgtaaaca tcaacgccgg cgggactgct cagcccgcgt
    18121 cggcacagcc agcaccgaac tgccatcgga ggaaccatga accattcatt cagctcccac
    18181 ttcgcgttcg acgacgcgat gaacgaggaa gcgaaaatct gcgtcgtcgg cgtgggtgga
    18241 ggcggcggca acgccgtcaa caacatgatc cggaggggca tccagggcgt agagttctat
    18301 gccgtcaaca cggaccagca ggcgctggcc gccaacctgg ccgccaacaa gatccaggcg
    18361 ggcgccggcc ttacgaaggg gctgggcgcg ggggcccggc cggccgtcgg ggccgaggcc
    18421 gtggaggaaa gccgtgacga gatcaagaaa gccctcaagg gcttcgacat ggtgttcatc
    18481 acggccggca tgggcggcgg caccgggacc gggggcgcgc cgatcgtcgc ggccatcgcg
    18541 aaggagctcg gcgtcctctc ggtcgcgatc gtgacccggc cgttcgagtg cgagggccgg
    18601 cgtcggcagc agtccgccga cacgggcatc gacctcctgc gcgcccacgt ggacacgctc
    18661 atcctgatcc cgaacgagcg cctgctcgac atcgacgacg gcaacacctc cctcctggag
    18721 gccttcgcga aggccgacga ggtgctctac aacgccaccc gcggcatcag cgacctcatc
    18781 acggtccacg gcctgatcaa cctggacttc gcggacgtga agacgacgat gcgcaacggc
    18841 ggcacggccc tgatggggtc cgcgacggcg tcgggcgaga agcgggcgga aaacgcggcc
    18901 acggaggcgc tctcgagccc gctgctggac ggcctctcca tccacggcgc ccgcaacgtg
    18961 ctcgtgaaca tcaccgcgaa ccagtccctg ggcatccggg aggccacggc cgcgacgaag
    19021 atcatccaga tggaggccgg cgacgacgtc gaggtgatct tcggcaccgt gatcgacgag
    19081 gagatgggcg acgagctgcg gatcaccgtc atcgccacgg gcttcgactc gcacggacgc
    19141 ggcgcacaga agaagggcca gggcaacctc gcactccggc tcgaccccgt cgagtacaag
    19201 ggagaacagg ccctctcgca cctggacgag ccggcgtacg agcgacgcgg ccggtcggta
    19261 tcgacgctcc ctccgtccca cgtgaacggc gacggggcgc ccgccggggg aacggcacgc
    19321 gtgcggcgcg tgcagaagga cgagatggaa gaccggacgg agcgcatcgc caagggaaac
    19381 gacgacgtgc cggccttcct gcgcaagatg ctcgactgag cggggccggc ccggtgccgg
    19441 caaagacctt cgaaacctcc attccacggc cgcccgtgag cagggcatcg atggccggac
    19501 gccgcaggct gtcggcgtga cggaacgggg gggttcggtg cggcgaccgg ccgcgtcccg
    19561 gggggggggc gcggccggcg cccgtttcgg ggggaagcgt tcctgcggac acgccgtaca
    19621 ttcccgccgg tcgctcggat gccgatgaga accttcattt cctgggcctt cgtcctcgtc
    19681 gcgctcgtcg cccaggcgtc ggcgcagcgc gccccgcgcc tgggggtcgt gtgggatccg
    19741 cctgccgatg ccctgcaggc cgaggccgac ctccgcgcga tccggtcgat gggtttcgag
    19801 gcggtgagga cggggctgat cgaccgggcg tcgcttctcg aactcgcgga cacgctcggg
    19861 atccacgtct accaggagat ccccctctcc ttcagcacgg cctccgagct gaccgacacg
    19921 ctgggcgtcg tgcgccgcct ggtggccgac gccctcgtgg cagccgagga tcacccgtcc
    19981 gcccgccacc tgggcttcgc gttcgcgccg tcgaccatcc aggaggacgt gtgtgccatc
    20041 ctggacgagc tcgccgactt tgccaggcgc cgggccgccc cggaggtcag gtactactac
    20101 gtctcgtggc tgccccacgc ggaccggtgc ggggaggccg tggacctggt ccttctcgac
    20161 gatccgatcc atgaccggcc cctgcggctc ctggaacggt ggacgggctc cgggccggtc
    20221 ggcctggccg cggtcggacg ccgcgtggat ccggaggcgg accgcggcct gctcgtggag
    20281 ggttccgaag agcggcaggc cctgtatttc gaggagacga ttccgcggct cctggaccgg
    20341 tccgatctct ccgtggcatt cctgtaccgg tggcaggatc cggacccgta cgattccggc
    20401 gcctttcacc cggcaccgtt cggcgccgtg tacggcctgc aggacgagac ggggcgcgcg
    20461 cgcatggccc gggacgtggc cgaggggctg ctcgcgcgtg gacagacgac cttccggctg
    20521 ctggccggca ccgctccccg gccgggcgct tcgtggttcg tgctgctggg gtggctcgtt
    20581 ctcgccaccc tctccgtgat ggttgccgcg tcaccccggt tccgcgcgat gattccgcgg
    20641 tatttcttcg cgcacggatt ctaccggcac gcgatccagg aggggcgcga cgtgctcacg
    20701 gtgatttcga ccgtcatgct cgtcctcctg ggcctgacga tcgggctgct cggggcgcag
    20761 gtcacgcgat tgctgaccga ttccgccccg ctgcacgccc tgtggtaccg cgcgggggcg
    20821 gagggacgat ccgcgatgga cgcgctgctg acggacgagg ccctgctcgt cgtcgcgatc
    20881 gggagcgcgg ggctcctcgt gctcgtggtc tgggtgttcg tgtggacgct ggtccggcgc
    20941 ggcgccgccc tgaagccgtc gcaggcgctg gcgacggccg tctggccccg atggccgatg
    21001 atgatcctcc tggtggcggc catggtggtc gacggactgg acgaagaggc cttccgacgc
    21061 tccatcgaat ggatgccggc tgcctggttc gccgtcgcgc tctggggaac catccgcacg
    21121 aacctcgacc tggcgcagat cgcgcgggtg aacccggcgg cgaccgtcgt gctgtggctc
    21181 gtgagcccgg tcgtggtgct gctcgggggc gccgtggcgt tcatgctcct ccggccggag
    21241 ctggccgcgc tcctgtgggc gctttggtcg gcctgatcag ttcccgacgc cgaggctttc
    21301 ggccggctcg gcatccgcgg cgatcccgtc gacgagcccg gtccaggtgc gcccggcaaa
    21361 gtcgacgtac ggctgcttgt accgcagcca tccgaagtgc agggcgaacg gcgtgacctg
    21421 catcgtgtcg ccgcgaccgg gcatgcgtgt caggaaggcc gtgatcgccc tcctggcccc
    21481 ctccgacgcc attccccgca tctcctgcac gcgccggtcg gtgtagtaac ggcaggtaac
    21541 ccgctcgaaa tcggggtcat gctcgacgag tccgggaacg aagtcgagct gcgggctcgt
    21601 ctggccctgg aaccagggga aaaccgggag atcgagccgg ctcatggcca tggccaggct
    21661 ccattcgcag gactcctccg tgcggccctc actgagtcgg ctcctgaaat gcgtctcctt
    21721 ccgccggtcc aggaagtcgc gcgccagcgc gcacgcctcg cgcgccaggg cgcggtccga
    21781 ggcgtagatc atgccggcct ggacccgcgg aaggtgggtc aggccgaacc ggcgcatcgt
    21841 gcgccgccgc cggtccagca ggtagtccgc caccacgccg attcccttcg ggccgccgaa
    21901 ccagaaatcc gccttctggt gcccggtcgc ggtgaacccg aaacccgaaa gctgcagcca
    21961 cagcgggtcg gggtcccggc accagaccat gtccgcgtcg gcgaacaggc agcggtcgaa
    22021 cggcatgaaa cggtccaggt gatgcttgaa gccgacgatg gaccggttct cctccggcag
    22081 cacctccaga accccgaaga gcccgtcgat gcccgccgac cgcaggagat ccaggtgaag
    22141 ctcggacgcg tagagggcga tcgggcgccg actgtcgtgc cgacggatcg tgacggcgga
    22201 cgccacggcc tggtgcaggt atcgggcggg gccgtacgcg tggagaacgt atccttcgga
    22261 catccgtcga gcggtcggta agcgcgcgct cttacgcggc tggcggaccc cggttcgggt
    22321 cccggatcag ccttccgcct ccacctccac gagtttttcc cgaaggcgct gcatcaggac
    22381 gtcgaacccg cgcttacgga tcatcgactg gaacgaccgg gcgtagccct ccaccgtgct
    22441 gacgtcgtcg atcgagacgt cggtcacgtg ccacccgccc tcgccgcggg ccatgtcgta
    22501 ggccaccgtc accagggcgt ccttcaccgt cgtcgacgtg acgacccggg ccgtgcttcc
    22561 gtcgacctcg atcgcgtcgt aggtgacctt ctggcggtag ggatccaggt tggagaggga
    22621 ttgcccccgg atgatcgacc cgaacaccga gacgaactcc gtgcgctggg cggccgacag
    22681 gtcgttccag tggggtccga gtgctccgcg gctcatggcc tcgaagtcga tgacggagtt
    22741 gatcacgtcg cgaagccgtt cgcgtcgctc ggggcttacc tcggaatccg cacggcccag
    22801 gagccgcttg atctcggcgt cccggctctc gagcatcgtg cggatctccg actgctgatt
    22861 ctgcgcggcg gccggcgtgg ccgccaggaa tgccgcgagg acgacggtgt ggagtatcga
    22921 ggacaggatt cgacgcatgg cgacggtctg gggtgttgaa cggcgcggtg ggcacgcctg
    22981 cagcacatca ctcaaggagc atgccgaacg cgtcgacgcg gtccaggagc ccggccgcgg
    23041 cgagcagccg gagcacggcc acgttgtagt cgaagacggc ctggtggcgg gcggcctgca
    23101 gctccaggtt ggtccggacg gccgagacca ggttcgcggt atcgcccagg tcgtagtcga
    23161 agttgacctg ttcggtccgc agccactcct tgctcaggtg cagggcctcc tccgccgccg
    23221 acacggcggc ctggcgggcc ttgagcgtgc gccaggcgga ctcgacctcg tagaggacca
    23281 gttgacgcgc ggcctcggac tggtgaacga cctcgtcgag ctgggcgcgg gcctgctcga
    23341 ccttcgcgcg ggtctgcagg aagttcaggt ccagccgcag gcccacgccg gcccggacgg
    23401 tgcgcgaaag gaagggatcc ccgaggtacg gattgtactg gcggtagcgg ccctcggccg
    23461 acgagtagcg ggcctcgacg cccaggaaga gcttcgggta aaaggcggaa cgggccaccc
    23521 cgacgagggc ccggcgtgcc gccacaccgg catcggcacg cgcgggctcg gggcgctccg
    23581 cgagggcgat ctcgaagtac cggtccagcg agtcgggcac gaaggggacc ggttccatgg
    23641 aggcgtcgcc ggggagcggc acgacgccgt cgggcagcat gagctggcgg cggagcccgg
    23701 attccacggt tgccaggcgc tcccggatct ccaccacccg tcgcccgaac tcctgctcgg
    23761 tgatcaggac ctggaaccgg tcggcgtcgt ccacgtccgg atcgccttcc tggagcagcc
    23821 gctcgatctc gcccttggca tcccggacgg ccttcgacgc ctcgtccgtg aggcgctcga
    23881 gtgaccgggc gagcaccagc ccgtagtaca gctcacccgt ccggagggcg acctggatcc
    23941 ggtccgagcg gacggcctgg acctccgcct cgaggccgtg ccgggcggca tcgatgctgc
    24001 gaccgatctc gccccaggtg aacagcggct ggatcgcctc gacctcgagc cggttgaacg
    24061 gccgcaggtc ctcccagtcg ttccggacgt cgggatccag gaagagccgg tccgtgggcg
    24121 tgtcgttcgg attgtccagg ccgggggcca ccgagtgggc ggacgtggcg cggaactcgg
    24181 tcaggaagcg gctcgcccgg gcgaggtcca ggcgggcgcc ggcgtaaccc actgccgcct
    24241 cgctcgagcg gatgtcgggg ctcacggcga ccgcgcggtc tatggcggcg gaaaccgtca
    24301 cccggaacgt gtcgggcacc ggctgccgcg cctgcgccgt cgtggtcacg agcagcgcga
    24361 cgagacacag ggtttttcgc atcatgcccg tgcaaatccc gcgttgcggt gaacgttccc
    24421 ggaccggggc ccccgcaggt ggtccgcgcc cgggcgcggg ggaacaggcc ccccgggccg
    24481 gcgtgagtac ggccggcatt ccagacaggg gacaaacccc ggagaccgat ggaccccgaa
    24541 gcaccgtgga aggaggtgag gacgctcggc gagctcaggg cttcgggcta ccgcgtccgg
    24601 tccgtgaagg acgagatgcg ctcgaacctc atccggcggc tgcgggaggg cgggccggtc
    24661 tttccgggca tcgtcggctt cgacgagacg gtggtgccgc agatccagaa cgcgatcctc
    24721 gggcgccacg acttcatcct gctcgggctc cggggccagg ccaagagccg gctgctccgg
    24781 atgctgccga cgctgctcga cgactacatc ccggtcatcg agggcagcga cctgaacgac
    24841 gatccgttcg cccccatctc caagtacggg cgtgacctgg tcgccgcgcg cggcgacgat
    24901 acgcgcgtgg agtggctgca ccgttcgcgc cggtacggcg agaagctcgc gacgcccgac
    24961 acgtcgatcg ccgacctgat cggggacatc gatccggtca aggcggccca tcatcgcctg
    25021 acattcgccg acgaggaagt gatccatttc ggcatcatcc cgcggacgaa ccgcggaata
    25081 ttcgccatca acgagctgcc cgacctgcag ccgcggatcc aggtgggcct gcttaacatc
    25141 atggaggagc aggacatcca gatccgcgga ttcaacatcc gctttccgct ggacgtcctg
    25201 atggtgttct cggccaatcc cgaggactac acgaaccgcg ggtccatcat caccccgctc
    25261 aaggaccgga tcgactccca gatcatcacc cactacccgc gcacgatcga gaccggcatc
    25321 gagatcaccc gcctcgaggc ctgggtggag cggggcgacc gcatgaccgt gcgcgtgccg
    25381 catgtctttc gcgagatcgt cgagcagatc gccttcgagg cccgggcgag cgaatacgtg
    25441 gaccagtcgt ccggcgtgtc cgcacggatg acccgcgcgg cgctcgagag cctcgtgtcg
    25501 gcggccgagc ggcgcgcctt cctgccgggc gccagggaga cgacggtacg tccgagcgat
    25561 ctctggttcg tgcagccggc cgttacgggg aaggtggagc tcgtgtacga gggcgagcag
    25621 gagggggccc aggcggtggc gcgtgcgctc gtcggccggg ccgtgaagcg gatcgccgcc
    25681 cgctacttcc ccgatccgga agacaagaag cgcggccggc cggcctatgc cgccgtgctc
    25741 ggctggtttt cctcgggcgg ttcggttccg ctccaccacg atctggatga tgccacctac
    25801 cggagccggc tcgaggaggt gccggggctc gccgacctgg cccgggactt cgccgaggag
    25861 gagcgggcgg tggcgatgga gctcgcgctc gaggcgctcc accagcactc gctcctgggg
    25921 aaggagtcga tgcgcgaagg cccgacgttc acggacatga tgggctcgat gctctcgtcc
    25981 ctgggcgccc cggaggacga cgcctgggac ggcgccttcg acgactcgcc cgacgaccgg
    26041 ccatgaggcg ccccaccgtg tggcgtgccc ggctggcgct cgcggcgctc gcggggctgc
    26101 ccctgccggc cctcgcgggg cagcagcggg atccgcggcc cccggaccat gacgccgtgc
    26161 tccagtgcgc ggcttcgcgg gccgggtacc tgcccgccac gtcggacgcg gacaagcgcg
    26221 cggcccgggc cggctccggg ttcgacgcgg agtattaccg gattgcgctc gacctcacgt
    26281 cccccgcgac gggcttcgtc gagggcgcga cgcgcatcgt gggccgtgcc acggcaccgg
    26341 tcctcgcgac cctggagctg gacttccatg tttcgatgct ggtggattcc gtggtttcgg
    26401 ccggggggac cggcctgtcg tggtcgtcgc tcggcaacat actggccgtg gacctggagc
    26461 cggccgcgac cgcgggcgag cgggtggacg tcacggtgca ttaccgcggc tggccagcgt
    26521 cgacgggctt cggcagcttc gcggccggga cgcgcggcgc cggggacccg tacgtctgga
    26581 ccctgagcga gccgtacggc gcgcgcacgt ggtggcccgg cgtggatcat ccgtcggaca
    26641 aggcggactc ggtggacatc gtggtgcgcg tcccggaccc gatgcaggtc gcctcgaacg
    26701 gactcctcgc gggcacggag ccgcacgggg acggcacgac cacgtggaca tgggagcacc
    26761 gctacccggt ctcgtcctat ctcgtgtccg tcgcggccgg cgtctacgac cactatctgc
    26821 aggagtacgt gcgcccggac agcctcgccg cgctcttcgg cgaggcctcg ttccccatag
    26881 agcatttcgc ctaccccggt tcgctggcgt tcgagggcac gcatccgcag acggggtggc
    26941 ggcgcgtggt ggacatcatg ccggttctgg agtggtggta cgggccctat ccgttccagg
    27001 acgagaagta cgggcacgcc catttcacct ggggcggggg catggagcac cagaccatca
    27061 cgtcgatggg agggaacagc gttggcctcg tcgcgcacga gctggcgcac cagtggtacg
    27121 gcgacgcggt gggtcccgcg acgtggccgc acctctggct gaacgaggga ttcgccacga
    27181 tgggcgagct catcgtctgg ggtgcgatga gcgacgtgta ccccggcgtg tacgagacga
    27241 tcttcaacct gtactacgac cgggccctgc gtgcccaggg gacgctcgtg ctggaggaca
    27301 cgtcgtcggt gagtgacatg ttcgccgcat cgcgcgtgta ctcgaagggc gggatggtgc
    27361 tgcacatgct gcgctcgatg accggcgacg aggtcttccg cgacgtcctg agggcgtggg
    27421 ccatgcaccc cgagttccgc tactcggtgg cgtcgacctc cgatttccgc cgcgtagccg
    27481 aggaggtgac gggactggac ctcgggcggt tcttcgagca gtgggtgacg tcggggaccg
    27541 gccaccccga gtaccgggcg acctggacca gcgcgcgcga ccgggaccgc tggatcgccc
    27601 gggtcacgct ggaccaggtg caggacgcga cggagtccgg cgtgttcgtg ttcgagatgc
    27661 cggtcgtgat ccggatcgtc accgactcgg gggagcacga ctacacggtc ctgaacgacg
    27721 agcgatcgca ggtcgtgcac gtggatcttg gcgacgagcc gcgcgaggtg atcatcgacc
    27781 cggaccgccg catcctgcgg gcccacgagg ccccggtcac gttcgaaggg gagaccccgc
    27841 ccgagttcct gccgtcggcg aggctcgttt ccatccatcc gaatccgtcc gacggggtgc
    27901 tccgcgtccg gatcgacgcc gtgggcgggc ggtcgggcac gatcgaggtg acggacgccg
    27961 tgggacggcg ggtacgcacg ctcgacgtca cgctcgcgcc gggaaggcag acgatcgatc
    28021 tggacgtcgg cgacctggcc gcgggggtct acgtcgtgcg gctcgtcgcg ggcgaagccc
    28081 gggactcgcg gacggtcgtg gtgatccgct gagtgtgtcc ctttctaccg ctccggaact
    28141 atgttgcccg agcagtcgat ggtctcgccc ggcccgggag cgcgggtcgg accgtcgccc
    28201 accgtgagcg gcggattgca ttccgcgtag ttcttgggca ggtagtgcga gtggctgccg
    28261 tggggaatct cgatccacga cttgctgtcg aacactccct gcgaatccat cattacgaac
    28321 gccaggaaga tcccgatgag gatcaggaga taggtgcgcg tgcgcttgct catgacgcgg
    28381 tggggaacgt cggacgggga acggggtgca gggacattcc gaaagctacg atccgatgca
    28441 tgggatctcc gcggcttttc ctctaattcg ccgcccgtca gaactccgca cgggcccatg
    28501 ctaacccggg tacccggttc atgatccgac gcgcggccat cctcctcgtg ctcctggcct
    28561 cgacgggcgc tgcgcgcgac gcctcggggc agctggccac ggtccggggt cgcgtcgtcg
    28621 aggcggagac gggggccgcg ttgcctgggg cgaccgtgca gctcacggat tcgaacggcg
    28681 cggagcgggg cgtatcggcc ggtccggacg gccggttcga gatgcgccgg ctgcctccgg
    28741 gcacctacgt gctcggggtg agcttcgtgg ggttcgagcc tcgggtggac accctccagg
    28801 tgggattcgg ggccacactg aacgtcaccg tccggctcga gacctcgcag aagcagctcg
    28861 aggaaatcgt ggtggaggag gacaggcgcg aggcccgtgt gggcgccggg gcctcgtcga
    28921 tccagccggc ggctctcgcc cgcgtgccga tgccggacgt gacgtacgac ctcgccggat
    28981 acctgctcac ccttcccgga ttcgtctcga cgggcgaccg gggcggacag ctcttcgtgc
    29041 gcggcggaac gcccacccag aacctggtgc tgatcgacgg catcccgatc ttccagccgt
    29101 tccacatcgt gggcttctac tcggcctttc cggcggacat cgtgtcgtgg gcagacgtgt
    29161 acgccggcgg attcaccgcg cgctacggcg gtcgcatctc cgcggtcgtg gacgtgacga
    29221 cccggaacgg cgacaagcgg cggctccggg cggcgggttc gatcgcgccg ttcctggcgt
    29281 cggtgcgcgc cgaggtcccc gtggccaggg accaggtgtc gctcgtcgcg agctggcgcg
    29341 agtccgtgat cgaccggatc gcgccggacc tgctcgggcg tggcctcccg ttccgcttcg
    29401 gcgaccgctt cgcgaagctc cacgcgtacc tgtcgcccac ctccagcttc tcgttcacga
    29461 tcctgaacac gttcgacgaa ggcgcgctcg acgtctccgg ctcggaagga tccacggccg
    29521 aggagagccc cgccgagtgg ggcaacaccg ctttcgggtc gcgctacacg tacatgccga
    29581 gcgaggcgcc cgtggtggcc cagttctcga tctactacac gcggctggtc agccggtacc
    29641 ggccgctcgc ccggcgtccc caggaatcgg acatccgggc ggtcacgatg gccgtggatt
    29701 tcgtgtacct gctgggcagc gtgcgcctgc agtcgggcat tttcggcaac ctgcaccagt
    29761 tcgtatacga cctcggcaac aggttcggcg gcgtgcggac cggagtgacg agcggcggcg
    29821 tgttcgtcga cgggcggttc gacgcggatt cgcggctcag gatcgagccg ggacttcgca
    29881 tggagacgtt ctcgcagggc atcagtaccg ccctcgatcc ccggcttcgc gtcaccctgc
    29941 tcaccctcgg cggccggcag cagtggacgc tggcctacgg ccggtatcgc cagcagattg
    30001 tgggggtgaa caacgagcag gacgtgtccg acgtcttctc ggtttgggcg ccctcgccgc
    30061 gggccacggc cgttccgcag gccgtgcact acctggccgg cgtcaacagc cgggtactgc
    30121 cctggctcga gctctcgctc gacggttacc ggaaggactt ctccaacctc accttccccg
    30181 tgttcaaccg ggagccgcag cattccggga gcttctcgct cgtggaggga gacgcgatgg
    30241 gcgtggacgt ccgcgccgag gtgaatcgcc cgtcgttcca tttctcgacg tcgtacagcc
    30301 tggccgacat cacgtaccgg tggtcgggac tctcccgggg cggccatacc atcgaggggc
    30361 ttacgaccgt cccggccccg gacgaccgcc cgttcgatcc gccgcatgac aggacgcacc
    30421 aggtgaacgc gatggcgcag gtgtcccgcg gaccgtggcg ggtctcggtg cggtggcagt
    30481 acggcaccgg gctcccgtac acgcggatca acgggttcta cgacggggtc cgggtgacac
    30541 cggatcccgc gcagccgcac ctggacgagc cgggcgagct cctggtgtcc cgcggagcca
    30601 ccctggactc ccgattcccg gcctatcacc ggctcgatct ctcggtcgag cgcgatttcc
    30661 gcttccgggt cgcgacggcc accctgcagg ccggcctgat gaacgcgtac gaccgcgcga
    30721 acctgttcga gtacaactac gtgaccggcg agcggatcga ccagcttccg ctcgtgccgt
    30781 c
//
LOCUS       DBNHQZ010000186         3147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2221902, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000186 DBNHQZ010000000
VERSION     DBNHQZ010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2221902"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="AC16YU_05010"
     CDS             <1..1803
                     /locus_tag="AC16YU_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR6064616.1"
                     /translation="DRNAPAAPPVSDPEWLARIVRELGDRDLDRALSWHLPDGFDVPA
                     VRGPADTRAGLDPVPGRPGGTPWEVREDLDPECFDRLDAAMETAVALGADSVGLVVRA
                     VSEGGLDSLAARVAGPVRHLVGGAHALVLAERLAASGALPTGGTLDLDPAAELLRHGP
                     AGRPFDLGRVADLLPPSAASSFRPACADGLTVHLGGATDTTELAVVLASVSDWLDALV
                     VQRGVDPESAASALHVVTSAGILYFPTIARLRATRLGIARVAGAYGVSDEGALRIPLH
                     VRTSPRQQSAFDPYGNLLRATVAATAAVVGGCDSLSVTPHDAGTAPSERGRRLALGLH
                     AVLREEGRLHLVSDPGAGSWYVEWLMARMGEEAWECFRQIEAEGGLQRSITEGGLAGR
                     IRAEGRAQEIAVDRRERIVIGVNRHADPAEVPPVEPGSSRRADSPRANGMLDFSRPSA
                     RLEALRAALHPESAHGHAHPPALLLAFGDAGPCGHRAAFVTDFLGCAGIRVEGPHHVD
                     TADEMTAAVRQSESPIILLCGPEDAFVVFGAAARNGLGGRDAGATLVAVGRPPDDDPG
                     RWFMDSSIDRDTPLLAGLGRILSAAGLRFEGERG"
     gene            1800..>3147
                     /locus_tag="AC16YU_05015"
     CDS             1800..>3147
                     /locus_tag="AC16YU_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR6064617.1"
                     /translation="MKRPDFTTIAFDPGLRPGGSAPGGRPAPSAEGIPIEPAYGPDAL
                     SGLDHLGAGAGAPPFVRGPYASMYAGRPWTIRQYAGFSTAEASNAFYRRNLAAGQMGL
                     SVAFDLATHRGYDSDHPRVEGDVGKAGVAIDSVEDMKVLFDGIPLDRMSVSMTMNGAV
                     LPVMAFFVVAAEEQGVAPESLSGTIQNDILKEFMVRNTYIYPPGPSLRIVGDIMAWAA
                     RRMPRFNSISVSGYHMHEAGAPADLELAYTLADGLEYLRTGLAAGLDVDDFAPRISFF
                     FAVGMHHFMEIAKLRAARLLWHEIVLPFGPKDPRSLALRTHCQTSGWSLTAQSPFNNV
                     ARTALEAMAAVLGHTQSLHTNALDEAMALPTDFSARIARNTQLVLQEETGIPHAVDPW
                     GGSYFVERLTADLADRARSLIAEVEQAGGMAKAIELGIPKRRIEEASARRQARIDSG"
BASE COUNT          418 a         1150 c         1109 g          470 t
ORIGIN      
        1 gataggaacg cccccgccgc gccaccggtc tcggacccgg aatggctcgc acgcatcgtg
       61 cgcgagctcg gggaccgcga cctggaccgg gccctctcct ggcacctccc ggacggcttc
      121 gacgttcccg ccgtccgggg accggcggac acgcgcgccg gcctcgaccc ggtgcctggg
      181 cgccccggag gcacgccgtg ggaggtccgc gaagacctcg atccggagtg cttcgaccgc
      241 ctcgatgcag ccatggagac ggccgtggcg ctcggcgcgg acagcgtggg gctcgtcgtc
      301 cgggccgttt ccgagggcgg gctcgattcc ctggccgccc gtgtagccgg gccggtccgc
      361 catctcgtcg gcggcgccca cgccctcgtc ctggccgagc gcctcgccgc ctcgggcgcg
      421 ctgccgaccg gaggcacgct ggacctcgac ccggcggcgg agctcctgcg gcacggaccg
      481 gccggccggc cgttcgacct tggccgcgtg gcggacctcc tgcccccgtc cgcggcctcc
      541 tcgttccggc ccgcgtgcgc ggatgggctg acggtgcatc tcggcggcgc gacggacacg
      601 acggagctcg ccgtcgtcct cgcctccgtg tcggactggc tcgatgcact cgtcgtgcag
      661 cgtggggtcg acccggagtc ggccgcctcc gcgctccacg tcgtgacttc cgcggggatc
      721 ctctacttcc ccacaatcgc gcgcctccgc gccaccaggc tgggcattgc ccgggttgcc
      781 ggcgcatacg gcgtctcgga cgagggcgcc cttcgcatac ccctgcatgt caggacgtcg
      841 ccccgacagc agtccgcatt cgacccctac ggcaatctgc tccgtgccac cgtggccgcg
      901 accgcggcag tcgtgggcgg atgcgacagc ctgtcggtca ccccccacga cgccggcacg
      961 gccccgtcgg aacgggggcg ccggctggcg ctcggcctgc atgccgtcct gagggaggag
     1021 ggccggctgc acctggtatc cgatcccggc gcgggatcct ggtacgtcga gtggctcatg
     1081 gcaaggatgg gcgaggaggc atgggagtgc ttccggcaga tcgaggccga gggcgggctg
     1141 cagcgctcga tcaccgaggg cggcctggcc gggcgcatcc gggcggaagg ccgtgcccag
     1201 gagatcgcgg tcgaccgccg ggagcgcatc gtgatcgggg tgaaccggca cgccgatccg
     1261 gccgaggtcc cgcccgtcga accgggttcg tcgcgccggg ccgacagtcc acgcgccaat
     1321 ggaatgctcg acttttcgcg tccgtccgcc cggctggaag ccctcagggc ggcgctgcat
     1381 ccggaatcgg cccacgggca cgcccatcct ccggcccttc tcctcgcgtt cggcgatgcc
     1441 ggaccttgcg gccaccgggc cgccttcgtg accgatttcc tgggctgcgc cgggatccgg
     1501 gtcgaggggc cgcaccacgt cgacacggcg gacgagatga ccgcggcggt gcggcagtcc
     1561 gagagcccga tcatccttct gtgcggtccg gaagatgcct tcgtggtatt cggggccgcg
     1621 gcccggaacg gtctcggagg ccgagacgcc ggggcgacgc tcgtggccgt cggccggccg
     1681 ccggacgacg acccgggccg ctggttcatg gactccagca tcgatcgcga tacgccgctg
     1741 ctcgcgggcc tggggcgcat cctgtcggcc gcggggcttc gtttcgaagg ggaacgcgga
     1801 tgaaacgccc cgacttcacg accatcgcct tcgatcccgg cctccggccc ggcggatcgg
     1861 caccgggcgg ccgccccgcc ccctccgccg aagggattcc gatcgagccc gcttacggcc
     1921 cggatgcgct ctcgggcctg gaccacctgg gggcgggcgc gggcgctccc ccgttcgtcc
     1981 ggggccccta tgcctccatg tacgcggggc gcccgtggac catccgacag tacgcgggat
     2041 tctcgacggc ggaggcgtcc aacgccttct accgccggaa cctggcggcc ggccagatgg
     2101 gcctgtcggt ggcgttcgac ttggccacgc accgcgggta cgactcggat catccccgcg
     2161 tggaaggcga cgtgggcaag gccggcgtgg cgatcgattc cgtcgaggac atgaaggtcc
     2221 tgttcgacgg gatacccctg gatcgcatgt cggtctcgat gaccatgaac ggtgccgtgc
     2281 tcccggtcat ggccttcttc gtggtcgcag cagaggaaca gggtgtcgct ccggaatccc
     2341 tctcggggac catccagaac gacatcctga aggagttcat ggtccggaac acgtacatct
     2401 acccgcccgg accctccctg cgcatcgtcg gcgacatcat ggcctgggcg gcacgccgca
     2461 tgccgcgatt caactcgatc tcggtcagcg gctaccacat gcacgaagcc ggcgcccctg
     2521 ccgacctgga gctggcgtac acgctcgcgg acgggctcga gtacctgcgg accgggctgg
     2581 cggccgggct cgacgtggac gacttcgctc cgcggatctc gttcttcttc gcggtcggga
     2641 tgcaccattt catggagatt gcgaagctgc gggcggcgcg cctgctctgg cacgagatcg
     2701 tgcttccttt cgggccgaag gatccccgca gcctggcgct ccggacgcac tgccagactt
     2761 cgggctggag cctgaccgcc cagtctccct tcaacaacgt cgcccggacg gcgctcgagg
     2821 ccatggccgc cgtgctcggc cacacgcagt cgctgcacac gaacgcgctg gacgaggcca
     2881 tggcgcttcc gaccgatttc tcggcccgga tcgcccggaa cacgcagctc gtcctccagg
     2941 aagagaccgg catcccgcac gcggtggatc cctggggcgg atcgtacttc gtggagcgcc
     3001 tgacggccga cctcgcggac cgcgcccgga gcctgatcgc cgaggtcgag caggcgggcg
     3061 ggatggcgaa ggccatagag cttggcattc cgaaacgccg gatcgaggag gcgtccgcca
     3121 ggaggcaggc ccggatcgac tcgggac
//
LOCUS       DBNHQZ010000187         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2898744, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000187 DBNHQZ010000000
VERSION     DBNHQZ010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2898744"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..821
                     /locus_tag="AC16YU_05020"
     CDS             201..821
                     /locus_tag="AC16YU_05020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6064618.1"
                     /translation="MNPNTNAWNRLRYGLYAPGYDVVARRMDGARRRSIGLVRLVPGE
                     RLLLVGAGTGLDLPWLPPGLEITAIDLSAPMLTRCETRARDLGLDVTCRLMDAHEMDL
                     PDARFDAVLLHLVLSVVPDPIAAAREVGRVLRPGGRAGIFDKFIAAGARPSPLRRIAG
                     AVANAIFSDINRELEPLLGAAGLETVHDEPSYGRGLFRIVVARKPA"
     gene            complement(831..1286)
                     /locus_tag="AC16YU_05025"
     CDS             complement(831..1286)
                     /locus_tag="AC16YU_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6064619.1"
                     /translation="MTTQTCARCGNPVPAGNPVCPYCGSFERVGGPRAAAAGQTRTVD
                     LGHRRLSAREAGERLQGEVERAAAAGERFLRLVHGHGSTGRGGVLRTALRGELARLRG
                     QGRIAAFIAGELLSGDTNEGRGFARRYPEARAWPDWNAGNEGVTIVVIG"
     gene            complement(1381..>1947)
                     /locus_tag="AC16YU_05030"
     CDS             complement(1381..>1947)
                     /locus_tag="AC16YU_05030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6064620.1"
                     /translation="TMPALLLLAALAACEEEVRPPRVGDPAPAFRLVADDGTEVSLSD
                     HRGKWVVLYFYPKDFSTGCTIQARNFQRDIDKYGARNAVIVGVGMDDPDSHARFCSAQ
                     GLSFRLLSDTEGVVSTAYGSIKGAGNSRFSERNTFVIDPEGTIQRVYLGVNPSTHSDA
                     VLGDLARFQGVEPGRQGNFSVDRLMPGG"
BASE COUNT          271 a          708 c          699 g          269 t
ORIGIN      
        1 tgctgggccc gcgccgggaa ggcggcgagc acggccgcga gggcaagaca cgcggttctc
       61 atggggtcgt gggcttgggc tccggagcga aggtagtgcc cgtccgggtt gcgatccgaa
      121 ggcgcgtggg tcgccgccgg atcccgggaa ggacgagcag gtattccggg ccgaggacgc
      181 agacccgtcg caaacgccgc atgaatccga acacgaacgc ctggaatcgc ctgcggtacg
      241 gcctgtacgc gccgggatac gacgtcgtcg cacggaggat ggacggcgcc cgcaggcggt
      301 cgatcgggct cgtgcgcctc gtgcccggcg agcggcttct cctcgtgggc gccggaaccg
      361 ggctggacct gccctggctt ccgccgggcc tcgagatcac cgcgatcgac ctgtcggcgc
      421 ccatgctcac gcgctgcgag acgcgggcga gggacctggg cctggacgtc acgtgccgcc
      481 tgatggatgc gcacgagatg gacctgccag acgcccgctt cgacgcggtt ctgctgcacc
      541 tggtcctctc ggtcgtgccg gatcccatcg ccgccgcccg ggaggtcggc cgggtgctcc
      601 ggcccggcgg ccgagcagga atcttcgaca agttcatcgc ggcgggcgcg aggccgtccc
      661 ctctccggcg gatcgcgggc gcggtcgcga acgcaatctt ctcggacatc aaccgggagc
      721 tggagccgct cctcggcgcg gccggcctcg agaccgtgca cgacgagcca tcgtacggcc
      781 gcgggctgtt ccggatcgtg gtcgcgcgga agccggcgtg agcgaatcgg tcacccgatc
      841 acgacgatcg tcacgccctc gttcccggca ttccagtccg gccacgcccg ggcttccggg
      901 tagcgccggg cgaatcccct gccctcgttc gtatcgcccg acaggagctc gcccgcgatg
      961 aaggccgcga tgcgcccttg cccgcgaagc cgcgcgagct caccgcgcag ggccgtccgc
     1021 aggaccccgc cccgcccggt ggagccgtgc ccgtgaacga ggcggaggaa gcgctcgccg
     1081 gccgccgcgg cccgctccac ttcgccctgg agccgctccc cggcctcgcg cgcggagagg
     1141 cggcggtgcc cgagatccac cgtccgcgtc tgcccggccg cggcggcccg gggcccaccc
     1201 acccgctcga agctgccgca gtacgggcag accgggttcc cggccgggac cgggttgccg
     1261 cagcgggcgc aggtctgcgt ggtcatggcg tgccgactcg gagttttcgc gcggcgtaga
     1321 agccgaatgc cctcaggaaa cggagattgt gcagtatatg gttggcgccc gggagcgccc
     1381 tcacccgccc ggcatgagcc ggtccacgga gaaattgccc tgccgccccg gctcgacgcc
     1441 ctggaaccgc gccaggtcgc ccagcaccgc gtcgctgtgc gtgctggggt tcacgcccag
     1501 gtacacccgc tggatcgtac cctcggggtc gatcacgaac gtgttccgct cggagaaccg
     1561 cgagttgccc gcgcccttga tcgagccgta cgccgtcgag acgaccccct ccgtgtccga
     1621 gagcaggcgg aacgagagcc cctgggccga gcagaatcgc gcgtgcgagt ccgggtcgtc
     1681 catgccgacg cccacgatga cggcgttccg ggccccgtac ttgtcgatgt cccgctggaa
     1741 gttgcgggcc tggatcgtgc acccggtcga gaagtccttc gggtagaaat acaggaccac
     1801 ccacttcccg cggtggtccg acaggctcac ctcggtgccg tcgtcggcga cgagccggaa
     1861 cgccggggcc gggtcaccca cgcggggcgg gcgcacctcc tcttcgcacg cggccagcgc
     1921 cgcgaggagc agcagggcgg gcatcgt
//
LOCUS       DBNHQZ010000188         6611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1139009, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000188 DBNHQZ010000000
VERSION     DBNHQZ010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1139009"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16YU_05035"
     CDS             complement(<1..583)
                     /locus_tag="AC16YU_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064621.1"
                     /translation="MTRSRMSVHPSARHVPSWALVLGLAAWLLPGGARAQVVDVDMAV
                     SGARTTLTFHVTWERSLRAALDSAGVNAVGPADLGKIGAGLAEAEHLVRLPSTAAPAA
                     RIVASESEAEPVPLAARTSAGDFAPFDVRVEGIGWLRRQPAGSVVVPLFRYDAASGAL
                     HRYHRIVVEVTTGAPLGRAVGAASAADNPHLLVD"
     gene            complement(633..935)
                     /locus_tag="AC16YU_05040"
     CDS             complement(633..935)
                     /locus_tag="AC16YU_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064622.1"
                     /translation="MTDLLRMESRQTLDETAFLGMVRARLASLYHDMNNPLAVASGNV
                     QYVGELLGMGDLEGISEALADVSEAHGQIEARLSDLLELRRTIEDRLTGLGPDDAG"
     gene            complement(994..1845)
                     /locus_tag="AC16YU_05045"
     CDS             complement(994..1845)
                     /locus_tag="AC16YU_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6064623.1"
                     /translation="MRIGDYTLRALEAGRFGLDGGAMFGIVPRPLWSRRIEPDAEGRI
                     PLATRCLLIEGPDRLVVVDAGIGDKVDARFREIYGIHPDGPDLVGPLAEAGFSPDDVT
                     DVILTHLHFDHCGGMTRMDGDRSVPVFRRAVHHVQGAHWDWAASRPLRERASFLPENF
                     RPVEAEGLLSRVDGAATILPGISVEPVHGHTEAQQLVHVEGGGEHLVFVADLFPTSAH
                     VPPVWGMAYDIRPLVTIEEKTSFLDRAIEGRWTLFFEHDASVQTGRIVAGDRGPELAD
                     PARLDER"
     gene            complement(1842..2501)
                     /locus_tag="AC16YU_05050"
     CDS             complement(1842..2501)
                     /locus_tag="AC16YU_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6064624.1"
                     /translation="MDRNLLPLFPLELVLYPEERLPLHIFEDRYKVMIRECIEGDRPF
                     GVVLARNDELAEVGCTASVVRVLREYDDGRMDIVVAGGRRFRLDHVVDDGPYLQGSVS
                     LFPERRGAADPMMRERAITQHMKLLELGGRMPSPVIYQNRPRLSFLIANNAGLSLEQQ
                     QKVLEMPDEGRRINYLVAHMEAFIPRVEEAEEVRRKVRSNGHFPDFPLGTGDATSGGD
                     A"
     gene            2643..3995
                     /locus_tag="AC16YU_05055"
     CDS             2643..3995
                     /locus_tag="AC16YU_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064625.1"
                     /translation="MPFPSRSLRALMTAILVVLPVAGVADAQPTLFGSLDDPSEAHRL
                     ARWDRTREVRLLGGLSLIGAQWRTAMAGRFHVEGARGSVLVEGALRAGIYGSYDPDHD
                     EAYDLARSLRYARLDPVGRQFAYVRVGPVRRGRLGLGHLMSFFDGEAAWDDRRVGLEM
                     RLGSENLALYALAQDLRLGNVVGGRATWRPLSGARSGTLRSVEIGGSWVTDRATRNLD
                     GVDPVVAWEADVRVEAARSGAFALFPFVSYARFTRYGEGLLFGADLESDNFIDLARLH
                     ARLAVHYGSDRFVPGYFGAFYPVSNLRDGIVEEDPIDFQSRAGVPLPDILKATSVLAE
                     SRIHLFERFEFWFAFRRHYGPQSLSTMHVRMFLRARRFRFELAQDRGGLRGLFSTARA
                     LGSEATLTFRTDYRLVSTVWIHAEARYTYERLPAGDGPGRRYLPQRRFEPYLGVRVPF
                     "
     gene            complement(4055..5878)
                     /locus_tag="AC16YU_05060"
     CDS             complement(4055..5878)
                     /locus_tag="AC16YU_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HIR6064626.1"
                     /translation="MTRALRISSLLLLLSLPAVPAAVGQSALPKYEFRGAWMATVINL
                     DWPTSQNYTTAQQQAQMREWLDKLKAAGINAVLFQVRDESDALFDSPYEPWSVFLSGV
                     QGQAPDPYYDPLAFAIEEAHKRGMELHAWFNPYRVWVSSRNYPRDPSSVYLAKPEWLL
                     TVDNAVTILDPGIPAVRAYLVDVIRDVVTRYDVDGVHFDDYFYPYPPNQIDGEDWTTY
                     QTYGGGFTSIATWRTYNVNMLVKGVWDMIQVEAPGVKFGISPFGIWKSGTPPGISGLS
                     GATDLYADAVVWMEQGWLDYLTPQLYWAFGGGQDYGKLAPWWADQVNGRHLYPGHGLY
                     RSDASTFSGALFSASEIPNQVRFNRAHDIPGSVFFRTRNITSFSSKGFADTLKTDLYR
                     HPALTPIMPWKDLRSPGVPTEFMVAASDGGVELSWRPAAPDGWSVEPRFYAVYRVQSD
                     LEPDWPAAMADPANLVNVTGDTTWVDPLELTPVRYHYTVTAVSSNSVESPPAVGASII
                     ARSPASDLPVAFRMHAAFPNPFRGATTVRYILPKTGRVAVSVHDVLGREVARLVDGAV
                     QSPAEYEVTWDAAASGVSAGTYFLVLRFEGAVESRPVAFVR"
     gene            complement(5975..>6611)
                     /locus_tag="AC16YU_05065"
     CDS             complement(5975..>6611)
                     /locus_tag="AC16YU_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064627.1"
                     /translation="GVIIPGMSAESFAWQPVTGYLWVSAGSPNDAPNLGHGQGVTNWL
                     SQTWYAFDASELAVDVVPVPKAFITWHIGEGEHADGRPRGQAFSLDGQLSYVVQFNQP
                     TNGLQVFSATPVAIEKLDDTVPDRFILMQAYPNPFNPSTNINFMLHEAGHARLAVYDV
                     SGREVAVLADQAMAPGTYQYTFDAAGLGSGVYLYRLEFGGQVATGRMTLVK"
BASE COUNT         1038 a         2287 c         2270 g         1016 t
ORIGIN      
        1 gatcgaccag gaggtgcgga ttgtccgccg cggaggccgc cccgactgct cggccgagcg
       61 gggcgcctgt cgtcacctcc accacgatgc ggtggtacct gtggagcgct cccgaggcag
      121 cgtcgtagcg gaagagcggc acgacgaccg acccggcggg ttgcctgcgc agccacccga
      181 tgccctccac gcgcacgtcg aacggcgcga aatcgcctgc gctcgtccgc gccgcgaggg
      241 gcaccggctc cgcctccgat tccgatgcga cgatgcgcgc cgcgggcgcc gcggtcgacg
      301 gaagccggac caggtgctcg gcctcggcaa gccccgcgcc gatcttcccg agatcggccg
      361 gtccgacggc gttgaccccc gcggagtcga gcgccgcccg gagcgaacgc tcccacgtga
      421 cgtggaacgt gagcgtggtc cgcgcgcccg aaacggccat gtccacgtcc accacctgcg
      481 cacgcgcacc gcccggcagc aaccaggccg ccaggcccag cacgagggcc cacgacggaa
      541 cgtgcctcgc ggacgggtga acggacatgc ggctccgggt catggcgcga actttgggga
      601 acgaatccgg agcggcacgc cgggttccgc ggtcatccgg cgtcgtccgg gcccaggccg
      661 gtgaggcggt cctcgatcgt gcgcctgagc tccaggaggt ccgacaatcg cgcctcgatc
      721 tggccgtggg cctcgctcac gtctgccagg gcctccgaaa tgccctcgag gtctcccatg
      781 cccagaagct cacccacgta ctgcacgttt ccggacgcca ccgccagggg gttgttcata
      841 tcatgataca gggacgcgag tcgtgcccgc accatgccca ggaacgccgt ctcgtcgagg
      901 gtctgccggg attccatgcg gaggagatcg gtcatggaga ggggtaacgc ctgaacctac
      961 gccccttgcg ggcgggcgtc aaccgcgccg gtattaccgt tcgtcgagcc gcgccgggtc
     1021 cgcgagctcc ggaccgcgat ccccggccac gatccggccc gtctggaccg atgcgtcgtg
     1081 ctcgaagaac agcgtccagc gcccctcgat cgcccggtcc aggaaggacg tcttctcctc
     1141 gatcgtgacg agcggacgga tgtcgtaggc catgccccag accgggggca cgtgggcgct
     1201 cgtggggaag aggtccgcga cgaagaccag gtgctcgccg ccgccctcca cgtgcacgag
     1261 ctgctgcgcc tccgtgtggc cgtgcaccgg ctcgaccgag atgccgggaa ggatcgtcgc
     1321 cgccccgtcg acccggctga ggaggccttc cgcttcgacg gggcggaagt tctccggcag
     1381 gaaagacgcc cgctcgcgca gcgggcgcga ggcggcccag tcccagtgtg cgccctgcac
     1441 gtgatgcacg gcccggcgga agacagggac cgagcggtcc ccgtccatcc gcgtcatgcc
     1501 gccgcagtgg tcgaagtgca ggtgggtcag gatgacgtcc gtcacgtcgt cgggcgagaa
     1561 gccggcctcg gcaagcgggc ccaccaggtc gggcccgtcc ggatggatgc cgtagatctc
     1621 gcggaagcgg gcgtccacct tgtcaccgat cccggcgtcc acgacgacca gacggtccgg
     1681 accctcgatg agcaggcagc gcgtggcgag cggtatgcgc ccctcggcat caggctcgat
     1741 ccggcgcgac cagagcggcc gcggcacgat gccgaacatg gcgccgccat cgagcccgaa
     1801 tcggccggcc tccagggcgc gcagggtgta atcgccgatc ctcatgcgtc accgccggac
     1861 gtggcgtcgc ccgtgccgag tggaaagtcg gggaagtgcc cgttcgagcg caccttcctg
     1921 cgaacctcct cggcctcctc cacccgggga atgaaggcct ccatgtgcgc cacgagatag
     1981 ttgatccggc ggccctcgtc cggcatttcg agcaccttct gctgctgctc cagcgacagc
     2041 ccggcgttgt tcgcgatcag gaaggacagc cgggggcggt tctggtagat gaccggcgag
     2101 ggcatgcggc cgccgagctc caggagcttc atgtgctggg tgatcgcgcg ttcccgcatc
     2161 atcggatcgg ccgcaccccg cctctccgga aaaaggctca ccgagccctg caggtacggc
     2221 ccgtcatcga ccacatggtc caggcggaat ctccgcccgc ccgccaccac gatgtccatg
     2281 cggccgtcgt catactcccg gagaacccgg acgacgctgg ccgtgcaccc gacctcggcg
     2341 agctcgtcgt tgcgggcgag cacgacgccg aacggccggt ccccctcgat gcactccctg
     2401 atcatcacct tgtagcgatc ctcgaagatg tgcagcggta gacgctcctc gggatacagg
     2461 acgagttcca ggggaaaaag cggcagtaga ttccgatcca ttcgcggcag tctggtcgca
     2521 ggcaggctga cggatcgctg atccgtccgg gtctatcgaa tgacgctccg tccgccgggt
     2581 ttcgacgcgc ggtgcgctct cgcggggggc cgtatcttcg caattcgaag cccggccccc
     2641 gcatgccctt tccgagccgt tcgctgcgcg cgctaatgac ggcgatcctc gtcgtactgc
     2701 cggtcgcggg ggtggccgac gcgcagccga ccctgttcgg gagcctggac gatccatccg
     2761 aggcgcaccg gctggcccgg tgggaccgca cgcgcgaagt gcgcctgctc ggaggcctgt
     2821 ccctgatcgg ggcgcagtgg cgcaccgcca tggcggggag attccacgtg gagggcgccc
     2881 gcggctcggt gctcgtcgag ggcgcgctcc gggcgggcat ctacggatcg tacgatccgg
     2941 atcacgacga ggcgtacgac cttgcccgct ccctccggta cgcgaggctc gacccggtcg
     3001 gccgccagtt cgcgtacgtc cgcgtcggtc ccgtgaggcg ggggcggctg ggcttgggcc
     3061 atctcatgag cttcttcgac ggagaggcag cgtgggacga ccgacgggtg ggcctggaga
     3121 tgcgcctggg gagcgagaac ctggcgctgt acgccctggc gcaggacctt cggctgggca
     3181 acgtcgtggg cggccgagcc acctggcgac ccctgagcgg cgcccggtcc ggcaccctcc
     3241 gctcggtcga gatcggcggc tcgtgggtga ccgaccgcgc cacgcgcaat ctcgacggag
     3301 tcgacccggt cgtggcctgg gaggcggacg tccgggtcga agcggcccgc tcgggtgcct
     3361 tcgcgctctt cccgttcgtt tcgtacgcgc ggttcacgag gtacggcgaa ggcctgctgt
     3421 tcggcgcgga cctcgaatcg gacaacttca tcgacctggc ccggctgcac gcgcggctcg
     3481 ccgtgcatta cggcagcgac cgcttcgtgc cgggatactt cggcgcgttc tacccggtga
     3541 gcaacctgcg tgacgggatc gtcgaggaag acccgatcga cttccagtcc cgcgccgggg
     3601 tccccctccc cgacatcctg aaggccacca gcgtgcttgc ggaaagccga atccacctgt
     3661 tcgagcggtt tgaattctgg ttcgcattcc gccggcacta cgggccgcag tccctctcca
     3721 cgatgcacgt gcggatgttc cttcgggctc gccgcttcag gttcgagctg gcccaggacc
     3781 ggggcgggct caggggcctc ttctcgaccg cccgtgccct gggaagcgag gccacgctca
     3841 cgttccgtac ggactaccgc ctggtgtcga ccgtgtggat ccacgccgag gcgcgctaca
     3901 cctacgagag gctccccgcc ggggacggac cgggccgtcg ctacctgccc cagcgccggt
     3961 tcgaaccgta cctgggcgtt cgggtgccgt tctgaggggt gcggggcggg ggtcctgccc
     4021 tgaccacggc acgccaagcc tccaggcggt ccgctcaccg cacgaacgcc accggccggc
     4081 tctcgaccgc cccctcgaag cgcagcacca agaagtaggt gccggccgaa acccccgacg
     4141 ccgcggcatc ccaggtcacc tcgtattccg cgggcgactg gaccgcgccg tccaccaggc
     4201 gcgcgacctc gcggccgagc acgtcgtgca cggaaacggc cacccggccg gtcttcggca
     4261 ggatgtagcg caccgtggtc gcgccccgga aggggttcgg gaaggccgcg tgcatgcgga
     4321 atgcaacggg gagatcggac gcggggctcc tggcaatgat ggacgccccc acggccggcg
     4381 gactctccac cgagttggac gacacggcgg tcaccgtgta gtggtaccgc acgggggtca
     4441 gctcgagcgg gtccacccac gtggtgtcgc cggtgacgtt caccaggttc gcgggatcgg
     4501 ccatcgcggc cggccagtcc ggctcgagat cgctctggac ccggtacacg gcgtagaaac
     4561 gcggctcgac ggaccacccg tccggcgcgg cggggcgcca ggacaattcg acccctccgt
     4621 cagaagccgc gaccatgaac tccgtcggca cgcccggcga tcgcaggtcc ttccatggca
     4681 tgatcggcgt cagcgccgga tgccgataga ggtcggtctt gagcgtatcg gcaaagccct
     4741 tcgacgagaa ggacgtgatg tttcgcgtcc ggaagaacac gctgcccggg atgtcgtggg
     4801 cgcggttgaa acgcacctgg ttgggaatct cgctcgcgga gaagagcgcg ccggagaacg
     4861 tggacgcgtc cgaccggtac agcccgtgcc ccgggtacag gtgccgtccg ttcacctggt
     4921 ccgcccacca cggggcgagc ttgccgtaat cctggccgcc gccgaatgcc cagtacagct
     4981 gcggcgtcag gtagtccagc catccctgtt ccatccatac gaccgcgtcc gcgtacaggt
     5041 cggtcgcgcc ggagagaccc gaaatgccgg gcggcgtgcc gctcttccag atgccgaagg
     5101 ggctgatgcc gaacttgacc cccggcgctt cgacctggat catatcccac acgcccttga
     5161 ccagcatgtt cacgttgtag gtgcgccagg tcgcgatcga ggtaaaacct ccgccgtagg
     5221 tttggtaggt cgtccagtcc tccccatcga tctggttggg cggataggga tagaaatagt
     5281 cgtcgaaatg gaccccgtcc acatcgtacc gggtgacgac gtcgcggatc acgtcgacca
     5341 ggtaggcccg caccgcggga atgccggggt ccaggatggt gaccgcgttg tcaacggtga
     5401 ggagccactc cggcttcgcc aggtacacgc tcgacggatc gcgggggtaa ttgcgggacg
     5461 agacccacac ccgatacggg ttgaaccagg cgtgcagctc catcccgcgc ttgtgggcct
     5521 cctcgatcgc gaaggcgagc gggtcgtaat acgggtccgg agcctgtccc tggacgccgg
     5581 acaggaagac ggaccagggc tcatagggcg agtcgaagag cgcgtccgat tcgtcacgga
     5641 cctggaacag gaccgcgttg atgcccgcag ccttcagctt gtccagccac tcccgcatct
     5701 gtgcctgctg ctgggccgtc gtgtagttct ggctcgtggg ccagtccagg ttgatcaccg
     5761 tcgccatcca ggccccccgg aactcgtatt tcgggagagc ggattggccg acggccgccg
     5821 gaacggccgg gagagacagc aggagcagaa gcgaagagat ccggagagcg cgcgtcatcg
     5881 tgcaggtcct atgggtttgc tcgtcgggct gcaggcagaa aagggcaccc ggcttgggac
     5941 cgggtgccct tctcatgcca accgcgccgg gtgattactt gaccagcgtc atgcggccgg
     6001 tggccacctg gccgccgaac tcgagccggt acaggtagac gcccgagccg aggcccgccg
     6061 cgtcgaacgt gtactggtac gtgcccggcg ccatggcctg gtccgccagg acagccactt
     6121 cacgccccga cacgtcgtag acggcgaggc gggcgtgccc ggcctcgtgc agcatgaagt
     6181 tgatgttcgt cgacgggttg aacgggttcg gatacgcctg catcaggatg aaccggtccg
     6241 gtaccgtgtc gtccagcttc tcgatggcca cgggcgtcgc cgagaagacc tgaagcccgt
     6301 tcgtcggctg gttgaactgg accacgtagg acagctgccc gtcgagtgag aaggcctggc
     6361 cgcgcgggcg gccgtcggcg tgctcacctt ccccgatgtg ccaggtgatg aacgccttcg
     6421 ggacgggcac gacgtcgacc gccagttcgg atgcgtcgaa tgcgtaccag gtctggctca
     6481 gccagttcgt gacgccttgg ccgtgtccca ggttcggcgc atcgttcggc gacccggccg
     6541 acacccagag gtagccggtg accggctgcc acgcgaaaga ctccgccgac atgcccggaa
     6601 tgatcacgcc c
//
LOCUS       DBNHQZ010000189         5310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2359528, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000189 DBNHQZ010000000
VERSION     DBNHQZ010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2359528"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="AC16YU_05070"
     CDS             <1..1256
                     /locus_tag="AC16YU_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6064628.1"
                     /translation="VAVAQRSRAPIQKLADRVAAWFVPTVVAIAVASFAAWALLGPEP
                     RFSHALINAVAVLIIACPCALGLATPTAIVVATGLAARLGILVREGSSIEQAGRIDRI
                     ALDKTGTLTEGRPAVAAVLPAGETSRERLLGVAASAEHFSEHPIARAILEEAEVSGLF
                     PSEPKRFESETGLGVRAHVDGRAVVLGRRRFLEESGVVLPPEEESAAAETEIWVAEDG
                     AVLGRILVADRVRDTAARAVELLRGLGVEPVMLTGDSPRAAEAVARTIGIRDVRAGVL
                     PHEKAGVVRALRDGGHRVGMVGDGINDAPALAQADVGIAMGSGTDIAVQASDMTLMRP
                     DPVAVVDAVRLSRRTLRVVRQNLFFAFVYNVILIPVAAGVLYPAFGLLLNPMIASAAM
                     AASSVSVVSNSLRLRRFQRTNPDPS"
     gene            1256..1462
                     /locus_tag="AC16YU_05075"
     CDS             1256..1462
                     /locus_tag="AC16YU_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6064629.1"
                     /translation="MDKELTIGGMHCHHCIHAVRRALSTVENVEIRDVEIGRARIHVK
                     DDELDEARVREALDEEGYSLEKVS"
     gene            complement(1464..2861)
                     /locus_tag="AC16YU_05080"
     CDS             complement(1464..2861)
                     /locus_tag="AC16YU_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6064630.1"
                     /translation="MSRPGTPDHPLRVAVVGAGPAGFYAAEHLFRQPDLAVEVDLFDR
                     LPTPFGLVRAGVAPDHQKIKSVTRVYDKTASNPGFRFYGNVDFGTDVSLGDLKRFYHA
                     VIFTTGAQVDRPLGVPGEDLPRSHSATEFVAWYNGHPDYRDLQFDLSQKSVAVVGVGN
                     VAIDVARILCKTPDELAVTDIADYALDALRGSRVEDVYVLGRRGPAQAAFTNPEIKEM
                     GELSDTDVIVRPEEARLDPLSEKEVAEADERSVAKKVEMIQAYAAAQPAGRRRRLHLR
                     FLVSPTEITPAADGGVGGMRIVHNELQPDGRGSLRPRPTGRFEDLEVGLVFRSVGYRG
                     VALPEVPFRDDWGVIRNEGGRVVDDSGAVAPGLYTAGWIKRGPSGVIGTNKACAVETV
                     NLLLEDLAGDGLPTPENPAADAADALVRARQPLVFDFEDWKKLDELEAAKGSALGRPR
                     VKFTTVREMAEALGR"
     gene            3212..>5310
                     /locus_tag="AC16YU_05085"
     CDS             3212..>5310
                     /locus_tag="AC16YU_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064631.1"
                     /translation="MGGKLQLRPFVVPGTIVLHAGGRILAASAWTVDEAAGRVTVNLP
                     AGTQVVATYRAFPLAVGPEFRLNREPLPVPERLARAAVRPGPSRARTQAGGLRSRGAI
                     SRGITAGSGRDASIESGLRLEVDGSPAPGVEVRAVLTDESTPIRPDGTTQRLSEFDRV
                     SVEIDSRPGQVRMGDVDVHLGASRFAAFSRKVQGVAVRSALGDPETAPVSLGIQAAGA
                     AARGIFHVQRITPIDGMQGPYRLEGREGELFVLAIAGSETVYVDGERLERGATLDYTI
                     DYSTAEITFMPRVPVTADRRIEVEFTYSINQYTRTVVATEGQAAFWPAPGGRHRATLG
                     VSWLREADGSRFTDEFGLSSADSLRLAAAGDAEAVRSGAIEVVFDPEAPYVQYRREVR
                     TGGDGSPDTVYVALEAAPMEGETVYRVRFSRVGTGLGRYAREGQSINGIVYVYRGAGG
                     GDYEPVRRLPRPELRRLLDLRGSVSPVEGLTVRAEWARSVNDLNRLSGVDSGDDAGDA
                     FAGTVELVLPVALGPGRLSGGASLVRRSAHFTSFDRTRTVEFGREWNLGRRAVDAVGS
                     IGSGGLESEADVHVAWAGGDSTLVRVEAGRLDVGDLFDGRRVRVSAHAYEPGLPGLRY
                     AVDRVGTDDFEFGERGHWELHRGRIEAPGTGRIRPYVEMEREIRRQRLDGDTLSALSI
                     GSVELRPGLAWTGDRASA"
BASE COUNT          709 a         1815 c         1981 g          805 t
ORIGIN      
        1 tggtcgccgt ggcgcagcgc tcgcgcgcgc cgatccagaa gctcgccgac cgcgtcgccg
       61 cgtggttcgt gccgacggtc gtcgccattg ccgtggcgag cttcgctgcc tgggcgctgc
      121 tcggccccga gccccgcttc agccatgcgc tgatcaacgc cgtggccgtg ctgatcatcg
      181 cctgcccgtg cgcgctcggc ctggccacgc cgaccgcgat cgtcgtcgcg acggggctcg
      241 cggcgcgcct gggcatcctg gtccgcgagg gctcgtcgat cgagcaggcc ggccggatcg
      301 accggatcgc gctcgacaag acgggcacgc tcaccgaggg ccgcccggcg gtcgccgcgg
      361 tgcttcccgc cggggagacc tcgcgggagc gcctgctcgg cgtcgcggcg tcggccgagc
      421 acttctcgga gcatcccatc gcgcgggcca tcctggagga ggcggaggtg tccggactct
      481 tcccgtccga gccgaagcga ttcgaatcgg agacgggcct gggcgtccgc gcgcacgtcg
      541 atggccgtgc ggtcgtcctg ggccgcaggc ggttcctcga ggagtcgggc gtcgtcctgc
      601 cccccgagga ggagtcggcg gccgcggaga cggagatctg ggtggccgag gacggcgcgg
      661 tcctcggccg catcctcgtc gcggaccgcg tgagggatac cgccgcccgc gccgtcgagc
      721 tgctgcgagg gctgggcgtc gagccggtga tgcttacggg cgactcgccg cgggcggccg
      781 aggccgtcgc gcggacgata gggatccgcg acgtccgtgc cggcgtcctg ccgcacgaga
      841 aggcgggcgt cgtccgcgcg ctccgtgacg gcggccaccg cgtgggcatg gtcggggacg
      901 gcatcaacga cgcgccggcg ctggcccagg cggatgtcgg catcgcgatg ggcagcggaa
      961 cggacatcgc cgtgcaggcc agcgacatga cgctgatgcg gcccgacccg gtcgcggtcg
     1021 tggacgccgt acgcctctcg cgccgcacgc tccgcgtcgt ccgccagaac ctgttcttcg
     1081 ccttcgtcta caacgtcatc ctgattccgg tggccgcggg cgtcctgtac ccggccttcg
     1141 gcctgctcct gaatcccatg atcgcgtcgg ccgcgatggc ggcatccagc gtgtccgtcg
     1201 tctcgaacag cctgcgcctc cgccgcttcc agcgaaccaa ccccgatccc tcctgatgga
     1261 caaggaactc acgatcggtg gcatgcactg ccaccactgc attcacgccg tgcgccgcgc
     1321 gctgtcgacc gtcgaaaacg tcgagatccg ggacgtggag atcggacgcg cccgcatcca
     1381 cgtgaaggat gacgaactgg acgaggccag ggtgcgcgag gcgctcgacg aggagggata
     1441 ctcgctcgaa aaggtgagct aactcagcgg ccgagggctt cggccatctc gcgcaccgtg
     1501 gtgaacttca cgcgtggacg tccgagcgcg gagcccttcg cggcctcgag ctcgtccagc
     1561 ttcttccagt cctcgaagtc gaacacgagc ggctgccggg cgcggacgag cgcgtcggcg
     1621 gcgtcggccg ccgggttctc cggcgtcggc aacccgtccc cggccaggtc ctcgagcagg
     1681 aggttcacgg tctcgacggc gcacgccttg ttcgttccga tgaccccgga ggggccccgc
     1741 ttgatccagc cggccgtgta caggccggga gcgacggctc ccgagtcatc gaccacgcgg
     1801 ccgccctcgt tgcggatcac gccccagtcg tcccgaaagg gcacctccgg cagcgccacg
     1861 ccccggtagc cgaccgagcg gaagaccagg ccgacctcga gatcctcgaa gcgtccggtg
     1921 ggcctcggac gaaggctccc gcgcccgtcc ggctgcagtt cgttgtggac gatgcgcatg
     1981 ccgccgacgc ccccgtcggc cgcgggcgtg atctcggtcg gggagaccag gaaccgcagg
     2041 tgcaggcggc ggcgccgccc ggcgggctgc gcggcggcgt acgcctggat catctcgacc
     2101 ttcttcgcca ccgaccgctc gtcggcctcg gccacctcct tctcggagag gggatcgagg
     2161 cgggcctcct cgggccggac gatcacatcg gtgtccgaga gctctcccat ctccttgatc
     2221 tcgggattcg tgaacgctgc ctgcgccggt ccccggcgcc cgagcacgta cacgtcctcg
     2281 acgcggctcc cgcgcagcgc gtcgagcgca tagtcggcga tgtcggtcac ggccagctcg
     2341 tcgggcgtct tgcacaggat gcgggcgacg tcgatggcca cgttgcccac gcccacgacg
     2401 gccacgctct tctgggagag gtcgaactgc aggtcgcggt agtccgggtg tccgttgtac
     2461 cacgcgacga actcggtggc cgagtggctc cgggggaggt cctcgcccgg aacgccgagc
     2521 ggacggtcga cctgggctcc cgtcgtgaag atcacggcgt ggtagaacct cttcaggtcg
     2581 ccgaggctta cgtccgttcc gaagtccacg ttgccgtaga agcggaagcc ggggttcgag
     2641 gccgtcttgt cgtagacgcg ggttacgctc ttgatcttct ggtgatcggg cgccacgccg
     2701 gccctgacga gcccgaacgg cgtcggcagc cggtcgaaga gatcgacctc gaccgcgaga
     2761 tcgggctgcc ggaacaggtg ctcggccgcg tagaagcccg cggggccggc accgacgacg
     2821 gcgacgcgaa ggggatgatc gggcgttccg ggtcgtgaca tgggtcgtga agggttggaa
     2881 tgcgctggct gggcccggca cacggttttt tcgggccgcg cagaaggtat catccgaccg
     2941 gagcggcgac aagcggggat gcacccacgc tccgaaaccg caccgggccg ccgaggtacg
     3001 ggagtcacgc cgtatccgaa ttccccggcc tgattgctcg attttccgct acgaccgtcc
     3061 ttctccgcag ctatctgacc gggtcatcgg gctcttggca ctgcgtattc tgtccgtttt
     3121 cgtcctttct tgggcgattc ttggccggcc gtcggctgcc caggagcgtg cgatcgtcgg
     3181 cacggcgcac gcggacacgc tgacgacgtc cttgggcggg aagctgcagc ttcgcccgtt
     3241 cgtcgtgccg ggcacgatcg tcctccacgc cgggggccgg atcctcgccg catccgcgtg
     3301 gacggtggac gaggcggcgg gacgggtcac ggtaaacctg ccagcgggga cgcaggtcgt
     3361 ggcgacgtac cgcgcgtttc cgctcgccgt ggggcccgag ttccggctga accgggagcc
     3421 gctccccgtg ccggagcgcc tggcgcgggc cgcggtgcgg cccggaccat cgagggcccg
     3481 gacgcaggcg ggcgggctca ggagccgggg cgcgatctcg cggggcatca cggcggggtc
     3541 cggccgggac gcatcgatcg agtccggcct ccgcctggag gtggacggca gtcccgcgcc
     3601 cggtgtcgag gtgcgcgccg tgctgacgga cgaatccacg cccatccgcc cagacgggac
     3661 cacgcagcgg ctctcggaat tcgaccgcgt gtcggtcgaa atcgattccc ggcccgggca
     3721 ggtccggatg ggagacgtcg acgtgcacct gggcgcgagc cgcttcgctg cgttcagccg
     3781 aaaggtgcag ggcgtggccg tgcgctcggc gctcggcgac ccggagaccg cgcccgtgtc
     3841 gctggggatc caggccgcgg gggccgcggc ccgcggcatc ttccacgtgc agcgcatcac
     3901 gccgatcgac ggcatgcagg ggccgtaccg gctcgagggc cgcgagggag agctcttcgt
     3961 gctggcgatt gcggggtctg agaccgtcta cgtcgacggc gagcgcctcg agcgcggcgc
     4021 gacgctcgat tatacgatcg actattcgac ggccgagatc acgttcatgc cgcgcgttcc
     4081 cgttacggcg gatcgccgta tcgaggtcga gttcacgtac agtatcaatc agtacacgcg
     4141 aaccgtcgtg gccacggagg gccaggccgc cttctggccg gcgccgggcg gaaggcaccg
     4201 cgcgacgctc ggcgtctcgt ggctgcgcga ggcggacggg agccggttca cggacgaatt
     4261 cgggctctcg tcggccgact cgctccggct cgccgccgcg ggggacgcgg aggcggtgcg
     4321 gagcggcgcc atcgaggtgg tcttcgatcc ggaggcgccg tacgtgcagt atcgccggga
     4381 ggtgcgcacg ggcggtgacg gctcgccgga caccgtgtac gtcgccctgg aagcggcgcc
     4441 catggaaggg gagacggtct accgcgtccg gttcagccgg gtgggcacgg gcctcgggcg
     4501 ctacgcacgc gaggggcagt cgatcaacgg gatcgtgtac gtgtatcgcg gcgcgggtgg
     4561 cggcgattac gagccggtac gccgtcttcc gaggcccgag cttcgccgcc tcctggacct
     4621 tcggggctcg gtctcgcccg tagagggcct gaccgtgcgg gcggagtggg cgcggtcggt
     4681 caacgacctg aaccggctct ccggcgtgga ctcgggggac gacgcggggg acgcattcgc
     4741 cgggaccgtc gaactggtcc tgcccgtggc gctcgggccg ggccgccttt cggggggagc
     4801 ctccctggtc cggcggtcgg cgcatttcac ctcgttcgac cgtacgcgca ccgtggagtt
     4861 cggccgggaa tggaacctgg ggcggcgggc ggtggacgcg gtcggctcga tcggctctgg
     4921 gggactggaa tcggaagccg acgttcacgt ggcgtgggcg ggcggggact cgacgctcgt
     4981 acgcgtggag gcggggcggc tcgatgtggg cgatctcttc gacggccggc gagtgcgcgt
     5041 atcggcgcat gcgtacgaac ccgggcttcc gggcctccgc tacgccgtgg accgcgtggg
     5101 cacggacgac ttcgaattcg gcgagcgggg ccactgggag ctgcaccgcg gccggataga
     5161 ggccccgggt acggggcgaa tccgcccgta cgtcgagatg gagcgggaaa tccgccggca
     5221 gcgcctggac ggggacacgc tttccgcgct gtcgatcgga tcggtggagc tccggccggg
     5281 cctggcgtgg acgggagacc gggcctcggc
//
LOCUS       DBNHQZ010000190         9240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1818020, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000190 DBNHQZ010000000
VERSION     DBNHQZ010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1818020"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1075
                     /locus_tag="AC16YU_05090"
     CDS             158..1075
                     /locus_tag="AC16YU_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064632.1"
                     /translation="MNSVWREYGVIGTLLVVAVGGYLLMSRQQKDVLGASLQSIGGHL
                     LALVPEAGGRSDVQAALDTLEERVRRGSVPPEQIERFAASVFNLGTSGDSLAADEAEM
                     VVRLAYSDPAALPIPRGEVPPPPAAPPRVSGEAEMDRLADELAPMVAFYETIKVTGDS
                     TGPAFRFYSDGKLHVVVDDRLREKIEGGEAAKVLMRDKAMSWRAHFSEAAEAERQRHL
                     EHAEKLARVAEVWADTLDLGPAAQVLARMSRLRSMGFVTEADVDTVMARIESEVNAIL
                     RKLPPPPPPAPGRVTSSVTVSSSSVSSTG"
     gene            1085..1522
                     /locus_tag="AC16YU_05095"
     CDS             1085..1522
                     /locus_tag="AC16YU_05095"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR6064633.1"
                     /translation="MKVAKQFRWEAAHRLPWHEGDCRHLHGHSYRMDVELSGPTDAHG
                     MVADFQEIKSWVRPLVAEWDHATLVAQSDETLLAVVREHGWKHAVLPYDTTAENLCRF
                     AAEYVLGAARERLARIGVSRIRVRVMETGTCYAEHDLTVESGD"
     gene            1515..2381
                     /gene="aroE"
                     /locus_tag="AC16YU_05100"
     CDS             1515..2381
                     /gene="aroE"
                     /locus_tag="AC16YU_05100"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6064634.1"
                     /translation="MTDLRLQGPLAVIGHPVAHSLSPGIHNAALRALGHAPAYEARDV
                     PPDALRDAVRRLVDEGFAGFNVTIPLKERILPLLDRLDGSARSVGAVNTVSIDRSGPV
                     PILTGHNTDVTGFLEPLARYADRLRGAPVLVWGAGGASRAVVAGVLRRLDPEVVTLVA
                     RRPDPVIALARDFAPGGGGRIAHSPWPAAPRVLDAHGLLVNATPLGMHPDMTSTPAPR
                     GEGLRSGQVVYDLVYRPAVTRLLADAAAAGADTIGGLPMLVAQAAEAFRLWTGLEMPV
                     DAVRRELTGAIP"
     gene            2475..4157
                     /locus_tag="AC16YU_05105"
     CDS             2475..4157
                     /locus_tag="AC16YU_05105"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR6064635.1"
                     /translation="MRLALAQINPTVGDLDGNARLIVRRAREAFEAGAGLVVFPELCI
                     TGYPPLDLLENPSFVRGAADALDQVTREVPPGIGVIVGCPVPNPEPVGKRLLNAALLL
                     EGGRVSGQAIKALLPTYDVFDEYRYFEPGTVCSVLEFRGLRLGLHVCEDMWNNEEQAP
                     YHLYRANPVDELAARGIDLFVNISASPFSVGKHAVRNRIVREICLEHGVPFVLVNQVG
                     ANTEVIFDGDSRVHAADGTLLRCAPSFEEALIVWDTDSDRADPVPGHSDVADLHDALV
                     LGIRDYVRKTGVFSKALVGLSGGIDSAVTCALAVAALGAGRVEGVTMPSAFSSGGSVT
                     DSVALAEALGIRIHRIGIGPAVEAFGSMLAPLFEGTDFGIAEENIQARARGTTLMAVS
                     NKFGYLLLTTGNKSELSVGYATLYGDMSGGLAVLADVFKNQVYALARHINERAGRSVI
                     PESTITKPPSAELRPGQKDSDTLPPYDVLDDILARYVEGRQDADHIVRETGHPDALVR
                     RVLDMVDRSEYKRRQAAPGLRVSPKAFGMGRRVPIVMRWTRRPATESDPVRP"
     gene            4154..4744
                     /gene="ruvA"
                     /locus_tag="AC16YU_05110"
     CDS             4154..4744
                     /gene="ruvA"
                     /locus_tag="AC16YU_05110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6064636.1"
                     /translation="MITRLSGSVLEKRPTEVVVDVQGVGYQVLIPTSTYESLPERGAR
                     ASLYTHLHVREDAMTLFGFATVDERAVFQMMLAVSGVGPKLALAALSAMRPAEIRAHV
                     SAGDVGFLTRIPGVGRKTAERLVVELRDRLEKLDLAAEGPAGTAAAGVRGDALAALEA
                     LGYSRSAAEKALRAAEKRHPDEASVEALIRLALREQ"
     gene            complement(4751..5827)
                     /locus_tag="AC16YU_05115"
     CDS             complement(4751..5827)
                     /locus_tag="AC16YU_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6064637.1"
                     /translation="MNGSSILDRLFLDWPLLSWLEAIGFAAVVAVVLVGARRLAAMYF
                     GRIADRTSNHVDDVVVAVIRGARAWFLALGALVVATSLLDAPEAAASAVHRLFFVALL
                     LQIGLWGNDLIRVVMTWYVEKQENGEVDPSRITAFRAFGLVGRIVLWSLLLILTLDNF
                     GVDITALVAGLGIGGIAIALAVQNVLKDILAYVSILVDQPFVIGDFLTVDELAGTVEH
                     IGINSTRIRSLSGEQLVFSNEDLLSSRIRNYKRMYERRVVFSVGVVYGTPVEQLEAIP
                     DMMREAIEAQPDVRFDRAHFKVFDDFALTFEGVYYVLLADYTEYMNRQQAINLALYRR
                     FTDEGIEFAFPTQTLHVESLPAAE"
     gene            5987..7813
                     /gene="typA"
                     /locus_tag="AC16YU_05120"
     CDS             5987..7813
                     /gene="typA"
                     /locus_tag="AC16YU_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6064638.1"
                     /translation="MNPSLRNIAIVAHVDHGKTTLVDAMLWQSGTFRDNQDVRERVMD
                     SMDLEREKGITIMAKNTAVNYGGVKINIVDTPGHADFGGEVERTLRMVDGVILLVDAA
                     EGPLPQTRFVLSKALELGLPAIVVINKIDRGDARPQEVLNEIYDLFIDLDASEVQIEF
                     PVLYAVARDGQCTRELGGTLTDLRPLFETILERVPAPVGDADSSLQVLVTNVVPDAYL
                     GPLAIGRVVAGTVRDRQAVTLCHRDGRRARAGVTALFTHQGLDRVPAGEAGPGEIVAV
                     AGMTGIGLGESIAEAENPVPLPALRIDEPTMSMEFRINDGPFSGREGRFVTSRNLRDR
                     LMKEARTNLAMRIEETDSADRFLVYGRGELQMAILIEQMRREGFEFCVGKPQVITRRV
                     DGAVHEPYELALIDVAEPYTGIVVQKMGIRRGQMTRMVNHGSGRVRLEFQVPSRGLIG
                     YRTEFLTDTKGTGILTHLFDAYRPWAGPIAHRASGALIADRTGKVTAYAVLNLQDRGE
                     LFAAPAEDVYEGMIVGENSRDADLEVNITKEKKLTNMRAASADSFEKLIPPRRLSLEE
                     CIEFIRDDELIEVTPVSIRLRKRHTDPNVRKRASMAPAESAQ"
     gene            complement(7807..8856)
                     /locus_tag="AC16YU_05125"
     CDS             complement(7807..8856)
                     /locus_tag="AC16YU_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6064639.1"
                     /translation="MRRHLFLALLFLIPPTGPAEGRQLALDRIADGPSVPPMTALAVS
                     AGGDVIAGAPSGVYRSSDGGRTWLAYDEGLAGLGRIVRAAFIDPEGTEWLGTEGGVYR
                     RDDSGGAWASVGLRYERVNAVTVAGRIVFAGTEHGLARSMDGGATWTVHAIGPERPSV
                     RTLAAHGSMVVAGTTDGVHLSHDAGRTWQPAGPGLEGAEVTVLLRAATGTLFAATRHG
                     RDGCSLFRSRSDGHLWSCANPDAITFRVEAMTGSAGGRLVVAGDRWVATSADEGHSWT
                     LHEAGSSMVRAVAALGHILLAGTADTGILRSSSDGAGFVPSNDGLVEGWARVDLLALE
                     SMRTPPLPAASETTY"
     gene            complement(8951..>9240)
                     /locus_tag="AC16YU_05130"
     CDS             complement(8951..>9240)
                     /locus_tag="AC16YU_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR6064640.1"
                     /translation="FRREGSDLHTVLRVNAFEAMLGAGRSIMTPYGTTLKVTVPKGTQ
                     PGDRLRLRGQGIRAGESAGDLIVELAVTVPKDLGSGDEEAIRQAAKKAGLL"
BASE COUNT         1419 a         3293 c         3163 g         1365 t
ORIGIN      
        1 cccatacggg gagcgtccgg cctttggtgg gaaggccgga cgcctttttt tgtgcccctc
       61 ccgcaatacg ccggggtcgc cgatcgtttt ccggaaacat cggggcaatc gaacgttttc
      121 atcgagcctc gaatcgcccg cgggacgtcc ccctcgcatg aactcggtct ggcgcgaata
      181 cggagtcatc ggtaccctcc tcgtggttgc ggtgggcggc tacctgctga tgtcgcgcca
      241 gcagaaggac gtcctgggcg cctcgctcca gtccatcggc ggccaccttc ttgccctggt
      301 gcctgaagcg ggcgggcggt ccgatgtgca ggccgcactg gacactctgg aggagcgggt
      361 tcgccgggga tccgttccgc cggaacagat cgagcgcttc gccgcatccg tcttcaacct
      421 gggcacctcg ggcgattccc tcgccgccga cgaagccgag atggtcgtcc ggctggcgta
      481 ttccgacccg gccgcgcttc ccatcccccg gggtgaggtg ccgccgccgc ccgccgctcc
      541 accgcgggtg agcggcgagg ccgagatgga tcgcctggcc gacgagctcg cgcccatggt
      601 ggcgttctac gagacgatca aggtcaccgg cgattccacg ggcccggcct tccgcttcta
      661 ctcggacggc aagctccacg tggtcgtcga cgaccgcctg cgggagaaga tcgagggcgg
      721 cgaggcggcg aaagtcctca tgcgtgacaa ggcgatgtcg tggagggcgc acttcagcga
      781 ggcggccgag gccgagaggc agcgtcacct ggagcacgcc gagaagctcg cccgcgtcgc
      841 cgaggtctgg gccgatacgc tcgatctcgg cccggcggcc caggtcctgg cccggatgag
      901 ccggctccgg tcgatgggct tcgtcaccga ggcggacgtg gacacggtaa tggcccggat
      961 cgagagcgag gtgaacgcca tcctccggaa gctgcctccg ccacccccgc cggcccccgg
     1021 ccgcgtgacc tcgtccgtta ccgtatcttc gtcatccgta tcgagcaccg ggtgacacgc
     1081 gcggatgaag gtcgcgaagc agtttcgctg ggaggcggcc caccgcctgc cctggcacga
     1141 gggtgactgt cgccaccttc acgggcattc gtaccggatg gacgtggagc tctccggccc
     1201 cacggacgcg cacgggatgg tcgccgattt ccaggaaatc aagtcctggg tccgccccct
     1261 ggtggcggaa tgggaccatg ccacgctcgt cgcccaatca gacgagacgc tcctcgccgt
     1321 cgtgcgcgag cacggctgga agcacgccgt gctgccgtac gacacgacgg ccgagaacct
     1381 gtgccggttc gccgccgaat acgtgctcgg cgccgcgcgg gagcgactgg cccggatcgg
     1441 cgtctcgcgc atccgggtcc gggtgatgga gaccggaacg tgctacgcgg agcacgacct
     1501 gaccgtggaa tccggtgact gacctccggc tccagggtcc gctcgccgtc atcggccacc
     1561 cggtggcgca ctccctgtcg ccgggaatcc acaacgccgc cctccgggcc ctggggcacg
     1621 cgcccgccta cgaggcccgc gacgtgcccc cggacgctct ccgtgacgcc gtgcgccggc
     1681 tggtggacga ggggtttgcc ggcttcaacg tcacgattcc gctcaaggag cgcatcctcc
     1741 cgctcctgga ccgcctggac ggatccgccc gcagcgtcgg tgcggtgaac acggtctcga
     1801 tcgaccggtc tgggccggtg ccgatcctca ccggccacaa cacggacgtt acgggattcc
     1861 tggagccgct tgccaggtac gccgaccgcc tgcgcggcgc gccggtcctc gtctggggcg
     1921 cggggggcgc ctcgcgcgcc gtcgtcgccg gcgtgctgcg ccggctcgat cccgaggtcg
     1981 tcaccctggt cgcgcggcgg cccgaccccg tcatcgcgct cgcccgggat ttcgcgcccg
     2041 gcggaggagg acggatcgcg cacagcccgt ggccggccgc gccccgcgtc ctcgacgccc
     2101 acggtctgct ggtgaacgcg acgccgctcg gcatgcatcc ggacatgacg tcgactcccg
     2161 caccgcgcgg cgagggtctc cggtccgggc aggtcgtcta cgacctggta taccgcccgg
     2221 ccgtgacccg cctgctcgcc gacgccgccg cggcgggcgc ggacacgatc ggcgggctgc
     2281 ccatgctcgt cgcccaggcg gccgaggcct tccgactgtg gaccggcctg gaaatgccgg
     2341 tcgacgcggt tcgccgcgag cttaccggcg cgatcccgtg accacgccct cccgggaacg
     2401 ccgcacttcc aggtggccgg ggccggcacc cgcgatgtcc gcgcgcgttg cacctcgatc
     2461 ccaaccggtt ttccatgcgc ctggccctgg cccagatcaa tcccacggtc ggtgacctgg
     2521 acggcaacgc ccggctcata gtccggcggg cccgcgaggc tttcgaggcg ggcgccggac
     2581 tcgtggtctt ccccgagctc tgcatcacgg gttaccctcc gctcgacctg ctcgagaacc
     2641 cgagcttcgt ccggggcgcg gcggacgcgc tggaccaagt gacccgcgag gtgccgcccg
     2701 gcatcggcgt gatcgtcggg tgccccgtcc cgaatccgga gcccgtcggg aagcggctcc
     2761 tgaacgccgc cctgctcctc gaaggcggac gcgtgagcgg gcaggccatc aaggcgctcc
     2821 tccccacgta cgacgtcttc gacgaatacc ggtatttcga gcccggcacc gtgtgctccg
     2881 tcctggaatt ccggggactg cgactggggc tgcacgtctg cgaggacatg tggaacaacg
     2941 aggagcaggc cccctaccac ctgtaccggg ccaaccccgt ggacgagctc gccgcgcgcg
     3001 gcatcgacct gttcgtgaac atctcggctt cccccttctc ggtcgggaag cacgccgtcc
     3061 gcaatcgcat cgtccgcgag atctgcctcg agcacggcgt cccgttcgta ctcgtcaacc
     3121 aggtcggggc gaacaccgag gtgatcttcg acggcgacag ccgcgtccat gccgcggacg
     3181 gcacgctcct gcgctgcgcc ccctcgttcg aggaggccct gatcgtctgg gacaccgact
     3241 cggatcgggc cgatcccgtg cccgggcatt ccgacgtggc cgacctgcac gatgcgctcg
     3301 tgctgggcat ccgcgactac gtgcgcaaga cgggcgtatt cagcaaggca ctggtcgggc
     3361 tctcgggtgg aatcgattcc gcggtgacgt gcgcgctcgc cgtcgcggcc ctcggtgccg
     3421 gacgggtcga gggcgtcacc atgccgtccg cgttctcgtc aggaggatcg gtcaccgact
     3481 cggtcgccct cgccgaggcg ctcggaatca ggatccaccg gatcgggatc gggcccgccg
     3541 tcgaggcctt cggatcgatg ctcgcgccgc tcttcgaggg cacggacttc ggcatcgccg
     3601 aggagaacat ccaggcccgg gcgcgcggca cgacgctcat ggccgtgtcg aacaagttcg
     3661 gctacctgct gctgacgacc ggcaacaaga gcgagctctc ggtcgggtac gccacgctct
     3721 acggggacat gagcggcggc ctcgcggtgc tcgcggacgt cttcaagaac caggtgtacg
     3781 cgctcgcccg gcacatcaac gagcgggccg gccgatccgt catccccgaa tcgacgatca
     3841 ccaagccgcc gtccgccgag ctgcggcccg ggcagaagga ctccgatacg cttccgccgt
     3901 acgacgtgct cgacgacatc ctcgcgcggt acgtcgaggg ccggcaggac gcggaccaca
     3961 tcgtccgcga gaccgggcat cccgacgccc tggtccgccg cgtactggac atggtggacc
     4021 gcagcgagta caagcgccgc caggccgccc ccggcctccg ggtgagcccc aaggcgttcg
     4081 gcatgggcag gcgcgtcccg atcgtcatgc gctggacccg aaggcccgca accgaaagcg
     4141 acccggtgcg accatgatca cccgtctgtc cggatccgtc ctcgagaagc gccccaccga
     4201 agtcgttgtc gacgtgcagg gcgtcggcta ccaggtgctc attccgacct cgacctacga
     4261 gtcgcttccc gagcgcggcg ctcgcgcctc cctgtacacg cacctccacg tgcgtgagga
     4321 cgccatgacg ctcttcggct tcgcgaccgt ggacgagcgc gccgtcttcc agatgatgct
     4381 cgccgtctcg ggcgtgggtc cgaagctggc actcgcggcg ctgtcggcca tgcggccggc
     4441 cgagatacgg gcgcacgtgt cggccggaga cgtgggattc ctgacgagga ttcccggcgt
     4501 cggccgcaag acggcggagc ggctggtcgt ggagctgcgg gaccggctcg agaagctcga
     4561 tctcgcggcc gagggtccgg cgggcacggc ggcggccggg gtgcgcgggg acgcgctggc
     4621 cgcgctcgag gccctggggt attcccgctc ggccgccgag aaggccctcc gcgccgccga
     4681 gaagcggcac ccggacgagg cttcggtcga ggcgctgatc cggctcgccc tgcgcgagca
     4741 gtgaggccga ctattcggcg gcgggcagcg actccacgtg cagcgtctgc gtggggaagg
     4801 cgaactcgat gccctcgtcc gtgaaccggc ggtagagcgc caggttgatg gcctgctgac
     4861 ggttcatgta ctctgtgtag tcggccagga gcacgtaata gacgccctcg aacgtcagcg
     4921 caaagtcgtc gaacaccttg aagtgcgcgc ggtcgaaacg gacgtccggc tgcgcctcga
     4981 tcgcctcgcg catcatgtcc gggattgcct ccagctgctc cacgggcgtg ccgtagacga
     5041 cacccacgct gaatacgaca cgccgctcgt acatccgctt gtagttgcgg atgcggctcg
     5101 agaggaggtc ctcgttcgag aagacgagtt gctcgcccga taggctgcgt atgcgcgtgc
     5161 tgttgatgcc gatgtgctcc accgtgcccg ccaactcgtc caccgtcagg aaatccccga
     5221 tcacgaacgg ctggtcgacc aggatcgaca cgtaggcgag gatgtccttc agcacgttct
     5281 ggaccgcgag ggcgatcgcg atgccgccga ttcccaggcc ggccaccagc gccgtgatgt
     5341 cgacgccgaa gttgtcgagc gtcaggatga gcaggaggct ccacagcacg atccggccca
     5401 cgaggccgaa ggcccggaag gccgtgatcc ggctcgggtc cacctcgccg ttctcctgtt
     5461 tctcgacgta ccacgtcatg acgacgcgga tcaggtcatt gccccagagc ccgatctgca
     5521 ggagcagggc cacgaagaac aggcggtgta ccgcggacgc cgccgcctcg ggcgcgtcga
     5581 gcagggaggt cgcgacgacc agcgcgccga gggcgaggaa ccaggcgcgg gcgccgcgga
     5641 tgacggcgac gaccacgtcg tcgacgtggt tgctcgtccg gtccgcgatg cggccgaagt
     5701 acatggcggc cagccggcgc gccccgacga gcacgacggc gacgacggcg gcgaagccga
     5761 tcgcctcgag ccacgacagg agcggccagt ccaggaagag gcggtccagg atcgacgatc
     5821 cgttcattga tgcaggggga gttcttccac aacggaggcc cattacacgc gcaaagcccc
     5881 gttttgaggc actgagcctc cgatcccgcg ataagttcac gcggcgcggg cagccgaacg
     5941 gcggatctgg cgtttcaaca ggctgtatcc gatctcgcta cccccgatga acccttccct
     6001 ccgcaacatc gcgatcgtcg cgcatgtcga tcacggcaag acgaccctgg tcgacgccat
     6061 gctctggcag agcggcacgt tccgcgacaa ccaggacgtc cgcgagcggg tcatggactc
     6121 catggacctc gagcgcgaga agggcatcac gatcatggcc aagaacacgg ccgtgaacta
     6181 cgggggggtg aagatcaaca tcgtcgatac ccccgggcac gccgacttcg gcggcgaggt
     6241 ggagcgcacg cttcggatgg tggacggcgt gatactgctg gtcgacgcgg ccgaggggcc
     6301 cctgccccag acccggttcg tcctgtccaa ggccctggag ctgggacttc cggccatcgt
     6361 ggtcatcaac aaaatcgacc ggggcgacgc ccgtccgcag gaagtgctga acgagatcta
     6421 cgacctgttc atcgacctgg atgccagcga ggtgcagatc gagtttccgg tgctgtacgc
     6481 cgtcgcccgc gacgggcagt gcacgaggga gctcggcggc acgctcacgg acctgcgccc
     6541 ccttttcgag acgatcctgg agcgtgtccc ggcgccggtg ggcgacgcgg acagctccct
     6601 gcaggtcctg gtcacgaacg tggtcccgga cgcgtacctc gggccgctcg ccatcgggcg
     6661 cgtggtcgcg gggacggtgc gcgaccgcca ggccgtcacc ctgtgccacc gcgacggccg
     6721 gcgcgcgcgg gccggcgtga cggcgctctt cacccaccag ggcctggacc gggtcccggc
     6781 cggggaggcg ggtccgggcg agatcgtggc cgtcgccgga atgaccggga tcggactcgg
     6841 cgagagcatc gccgaggcgg agaatcccgt tcccctgccc gcgctgcgca tcgacgagcc
     6901 cacgatgtcg atggagttcc ggatcaacga cgggccgttc tccgggcgcg aggggcggtt
     6961 cgtcacgtcg cgaaacctgc gtgaccgcct catgaaggag gcccgcacca acctggccat
     7021 gcgcatcgag gagacggact cggccgaccg gttcctggtc tacggccggg gcgagctcca
     7081 gatggcgatc ctcatcgagc agatgcgccg ggagggattc gagttctgcg tcggaaagcc
     7141 gcaggtcatc acgcgccggg tggacggcgc ggtccacgag ccctacgagc ttgcgctcat
     7201 cgatgtcgcc gagccgtaca ccggcatcgt ggtccagaag atgggcatcc gccgcggaca
     7261 gatgacgcgc atggtgaacc acggctcggg gcgcgttcgc ctcgaattcc aggtgcccag
     7321 ccggggactg atcggctacc gcacggagtt cctgaccgac acgaagggca cgggcatcct
     7381 cacccacctc ttcgacgcgt accggccgtg ggccgggccc atcgcgcacc gcgcgtccgg
     7441 agcgctcatc gcggaccgga ccggcaaggt caccgcgtac gccgtgctga atctgcagga
     7501 ccggggcgag ctgttcgcgg cgccggccga ggacgtctac gagggcatga tcgtcggcga
     7561 gaacagccgc gatgccgacc tggaggtgaa catcacgaag gagaagaagc tcacgaacat
     7621 gcgcgccgcg tcggccgact ccttcgagaa gctcatcccg ccgaggcgcc tgtcgctcga
     7681 ggagtgcatc gagttcattc gcgacgacga gctcatcgag gtcacgccgg tctccatccg
     7741 gctgcgcaag cgccacacgg acccgaacgt ccgcaagcgg gcctccatgg cccccgcgga
     7801 atcggctcag taggtcgtct cggacgcggc cggaagcggc ggcgtcctca tgctctcgag
     7861 cgcgaggagg tcgaccctcg cccatccctc gacgaggccg tcgttcgacg gcacgaagcc
     7921 cgccccgtcc gaggaagagc gcaggatccc cgtgtctgcc gtcccggcga gcaggatgtg
     7981 ccccagcgcg gccacggccc gcaccatcga cgagcccgcc tcgtgcagcg tccacgagtg
     8041 tccctcgtcc gccgacgtgg cgacccaccg atcccccgcc accaccaggc ggcccccggc
     8101 cgaaccggtc atggcctcga cccggaacgt gatcgcgtcg gggttggcgc aagaccacag
     8161 gtgaccgtcc gagcgggagc ggaacagcga gcagccgtcc cggccgtgcc gcgtcgcggc
     8221 gaacagcgta ccggtggccg cgcgcagcag gacggtgacc tcggcacctt cgagccctgg
     8281 gcccgccggc tgccaggtcc gccccgcgtc gtgcgagaga tgcacgccgt cggtcgtccc
     8341 ggcgaccacc atggatccgt gcgcagcgag cgtacgcacc gaggggcgct cgggcccgat
     8401 cgcatggacc gtccacgtgg ccccgccgtc catggagcgg gcgagcccgt gctccgtgcc
     8461 cgcgaagacg atccgtccgg cgacggtgac cgcgttcacg cgctcgtatc gcaggccgac
     8521 cgatgcccag gcgcctccgg agtcatccct ccggtacacg ccgccctcgg tcccgagcca
     8581 ttccgtgccc tccgggtcga tgaacgccgc gcggacgatg cgtccgaggc ccgcgagtcc
     8641 ttcgtcgtac gcgagccagg tgcggccccc atccgaggag cgatagacgc cgctcggcgc
     8701 gcccgcgata acgtcaccgc cggccgatac cgcgagcgcg gtcatgggcg gcacactggg
     8761 accatccgca attcgatcga gtgccagctg gcggccttcg gccggcccgg tgggcgggat
     8821 caggaagaga agcgcgagaa agagatgccg ccgcacggac cgctcgatat ggtggtgggt
     8881 acccctcctg tatcgaacgg gtcgtgcacg actttaagcc gccatgcgga tcgggtccgc
     8941 ggcccctctc tcagagcagc ccggccttct tcgcggcctg ccggatcgcc tcctcgtctc
     9001 cgctcccgag gtccttcggg acggtcacgg cgagctccac gatcagatcg ccggccgact
     9061 ccccggcccg gattccctgg ccccggaggc gcagccggtc gcccggctgg gtgcctttcg
     9121 ggacggtgac cttgagggtc gtgccgtacg gagtcatgat gctccgcccg gccccgagca
     9181 tcgcctcgaa cgcgttcacg cgcaggaccg tgtgcaggtc gctgccttcc cggcggaagc
//
LOCUS       DBNHQZ010000191        10362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1008644, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000191 DBNHQZ010000000
VERSION     DBNHQZ010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1008644"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="accD"
                     /locus_tag="AC16YU_05135"
     CDS             complement(<1..798)
                     /gene="accD"
                     /locus_tag="AC16YU_05135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR6064641.1"
                     /translation="MTWFKRTKAGIRTEVTEQNQTPEGQWVKCPGCGETVNRRGLERQ
                     LLVCPKCDHHFTMGSEAYFRMIFDEGVYEKVDTNLVSTDPLGFTDTRPYPVRLSQARK
                     KTGLSDAVQAAVGTVGGHRMSVACMDFSFVGGSMGSVVGEVISRAIRRSYTERIPLLI
                     ITQSGGARMMEGALSLMQLAKTSAHLSRLAEQDVPYFVLLTNPTTGGVTASYAMLGDI
                     HLAEPGALIGFAGPRVIRETMGQDLPQGFQRSEFLRDHGFVDQIVDRR"
     gene            complement(818..1534)
                     /gene="tsaB"
                     /locus_tag="AC16YU_05140"
     CDS             complement(818..1534)
                     /gene="tsaB"
                     /locus_tag="AC16YU_05140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR6064642.1"
                     /translation="MSVILGIETATDVCAVAVDVDGSVVCSVSLDRPRRHAESLVPMI
                     GAALRAAGLRADDLQAVAVSAGPGSYTGLRIGVSTAKGICVATSAGLLPVPSLEALAL
                     ATGAEPGRIVVAAFRARRAEVYAASYVIGPDGLPRTLHGPVAGPPGDALQELGASPSG
                     PVVVTGDGGRLVSAALRALGTPHVLDPVTRPAASAVVRLGRRIGAGSGPVDVERFEPY
                     YLREFTARPAAGSVFDRLPF"
     gene            1757..3880
                     /locus_tag="AC16YU_05145"
     CDS             1757..3880
                     /locus_tag="AC16YU_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6064643.1"
                     /translation="MNVYETDRIRNVALVGHQGAGKTMLAEAMLRTSGAIQRMGSIAE
                     GTTVSDYHPSEKERQMSVFASLLQVEWDGVKINVLDTPGYPDFVGEVIASLHVADAAI
                     FVINAAEGVQVGTELAWRTATELGKPSMFVINHLDKSDTSFRAVVEQIRDRFGRAASI
                     VQIPAGEGTREIVDVLTMKQAGFAPDTGKMTEQEIDAPHREEAERLHNELIESIAEND
                     EGLMDLYFEKGSLTEDEMRKGLHEELIHRQLFPIFVTSATQNIGVSRLMGFIRNVLPN
                     PAEMPPAPLENGGTLACDARGEPVAFVFRTMAEAHVGDYSFVRVMSGTLSQGDDLVNT
                     HSGASERLGQLFSITGHERDNVARLVAGDIGALVKLRNTHTNDTLLRKGSKSSVMPIR
                     FPDPRYATAVIATREGEEDKLAQGLHQLANEDPSLVITHDQHLSQLVLSGQGEMHLEI
                     ARFRLRNRFGVDIEYIRPRVPYRETVQQQARSKYRHKKQTGGAGQFADIAMLVEPLVG
                     DFAPPPDISVRGEHSEKLAWGSTIQFIDAIVGGVIDMRRFFGAIQKGVMEALQKGPIA
                     GYPVGDIRVVIYDGGMHSVDSNEAAFKTAARMCFRAAFREANPILLEPIHNVEVLVPD
                     AFTGDVMGDMNTRRARIQGIDSEGPFQKIVAQVPEAELHRYSTSLRSMTQGRGLHRAV
                     FSHYEAMPRHVQEKVVADAVAEQEE"
     gene            4072..5127
                     /locus_tag="AC16YU_05150"
     CDS             4072..5127
                     /locus_tag="AC16YU_05150"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR6064644.1"
                     /translation="MDTDRFRTSERARRSMLRAVYEENRRIAEDDARIREVRRRRYIG
                     GVGMLVVAVGAVVIIAGESSSLRAPDRSDMASPTAILGADLPVVDTSDAEDEWVPEEE
                     YRALLAQETPLAALFNLRVRTIVLDPGHGGVDPGAVGPAGTLEKDITLDVALRLARRL
                     EDHGYRVLMTRKDDRTISLRERVEFANSRPTDLFVSIHLNELPVDSVNVVETYFFGAN
                     TESRSLRVAEKENSGADYTVADFNGMIRRVSTTMKLQESRQLARAIQRSVYTNKRQLD
                     LEVANWGVKSGPFVVLLGSEAPSVLAEIGVLSHPEDERRLQSAAHREQLATFMEEGIL
                     TYLISRTRDGSGSETAQ"
     gene            5129..6178
                     /gene="mamK"
                     /locus_tag="AC16YU_05155"
     CDS             5129..6178
                     /gene="mamK"
                     /locus_tag="AC16YU_05155"
                     /inference="COORDINATES: protein motif:HMM:NF040964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005856 - cytoskeleton [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MamK family actin-like protein"
                     /protein_id="HIR6064645.1"
                     /translation="MATKKQTSKKKADASAGEALYIGIDLGTSRSAIVASNGRREWME
                     SYVGWPRDFVSRKMLGRPVLYGREAIDNRLSLNLVRPLEQGVIRDGTERAEEAVRQMV
                     HHLIEKVDPGSATEIYAAVGVPAEALRVNKLAIRNAVKEYADSLMVVSEPFAVAYGLG
                     TLNNALIIDIGAGTTDFCIMHGTMPGDDDQRTLTIAGDFLDQQLMERLLERFPEASVS
                     PTMARGFKEEYGFVGNHRDRVKVKVPIQGATNAIDITSDLRKTCETLLPVLAEATMDL
                     IARYEPDFQEEVKQNVFLAGGGSQIKGLAEALGESLAPQGNFNVRVIDDPLFAGAEGA
                     LALAQDMPPEYWEDM"
     gene            complement(6231..6304)
                     /locus_tag="AC16YU_05160"
     tRNA            complement(6231..6304)
                     /locus_tag="AC16YU_05160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6268..6270),aa:Met,seq:cat)
     gene            6400..7563
                     /gene="holB"
                     /locus_tag="AC16YU_05165"
     CDS             6400..7563
                     /gene="holB"
                     /locus_tag="AC16YU_05165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR6064646.1"
                     /translation="MSWDRVIGQPRVVDTLRRAVERDRVAHAYLFHGPDGSGKRAAAL
                     VLAQALLCERSDGRPCGTCSSCTRARRLVHPDLQFLFPQPADVSMDSVRERLDRIAAD
                     PYATVDFVRRSSLDDPEAVSNRVVQYSVARINEEVRRAMSFRSVEGGYKVAIVTDAEA
                     LRKEAANAFLKLLEEPTPNTVFILTTDRMDRLLPTIVSRCQQVRFDRLGDEDVARGLA
                     TRLGLPDTVAATIARMADGSYSRALELAASEELLESREAAVEFLRAAYLAWSDVDRMS
                     QRVEELVRRSREHQKFQLGLMLLWIRDVVLYRERGADAAIVNVDQARAISNFIEGVPN
                     ADQTAMVELVEGAIELVERNVQSRLVLTTLAQKLALAMRGRGDGRLYVPLTDD"
     gene            7639..8640
                     /locus_tag="AC16YU_05170"
     CDS             7639..8640
                     /locus_tag="AC16YU_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR6064647.1"
                     /translation="MACASGACGSGGSAGGSGCGSGRCGTDDCPSTLDTDWLAGIDSA
                     RDEPPFPIVEVAFKGGRRGHFRITGETRLHAGDPVIVEADRGIDYGTVHLGGPMVRLR
                     LRSHGQDENGEYPAVLRRATPEDTERWEANRERDTETFLVGRRTIDRMNLSMKLVDVE
                     WQLDGRKVTFYFTAEQRVDFRELVRRLARRFRTRVELRQIGARDEAARIGGIGSCGRE
                     LCCSTWLQEFRPVSTQAAKSQNLPLNPVRLSGQCGRLKCCLNYELEQYLQALARFPAV
                     DTPITIASRMGVIRKVDIFRERLWVLLEGGEWTDIGLDTAREALGIERDPPPAEPRD"
     gene            complement(8654..9058)
                     /locus_tag="AC16YU_05175"
     CDS             complement(8654..9058)
                     /locus_tag="AC16YU_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6064648.1"
                     /translation="MSEIHLVGDRVLISPDEGERQTESGLVLPATVAEREKVRSGRVV
                     RVGPGYVIPNPEYSEGEPWAPSRDAVRYLPMQARPGDYAFFLRKEATELAWEGRTYLI
                     VSHGAILALVRDDPDDLLQSIAPDDEDAEDIA"
     gene            complement(9055..>10362)
                     /locus_tag="AC16YU_05180"
     CDS             complement(9055..>10362)
                     /locus_tag="AC16YU_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6064649.1"
                     /translation="CAHPVPSQPRFNRRATHPVAEALRLLVIVNVYRPDLGGGVLFAD
                     LCEGLAARGFDVTVRCAYPYYPEWRDKTGRNGLRIERARENGVLVERYGLFIPARPNR
                     LFPRLLYEASFFASLARDVPDPGRFDAVMAFCPLVGAIAFGAVAARAAGAPLWLNVQD
                     IPVDAAAASGIVRSGRVARWMSRLQRALFNRADTWTTISPVMAERLEPLRRGSQPLHT
                     IPNWLHASLAAEIAALPGKLGRPPGRPVRLLYSGNIGTKQDLVGFCSTLRHSDAAFEF
                     RIQGGGGMAQAVRDWVQGTGDPRFTFHDLTDEAGLARALSDADLFVVTETHGAGGSFI
                     PSKILPSMASGTPLLVVCDADSPLGREMDEAGAGPVFRWDAAHEVGSMLANLPDRPGV
                     FSDWQDNALRRAALFDRNTIMDRYADLLTRLTETARSRPERLP"
BASE COUNT         1711 a         3419 c         3657 g         1575 t
ORIGIN      
        1 gcgacgatcg acgatctggt ccacgaaccc gtggtcccgg aggaactcgg agcgctggaa
       61 gccctgcgga aggtcctgtc ccatggtctc gcggatcacg cggggtcccg cgaacccgat
      121 gagcgcgccc ggctccgcca ggtggatgtc gccgagcatc gcgtacgagg ccgtgactcc
      181 gcccgtcgtc ggattcgtca ggagaacgaa atacgggacg tcctgctcgg ccaggcgcga
      241 gaggtgggcg ctggtcttgg cgagctgcat caggctcagc gcgccctcca tcatgcgcgc
      301 gcccccgctc tgcgtgatga tgagcagcgg gatcctctcc gtgtacgatc ggcggatggc
      361 ccgcgagatc acctcgccga ccacggatcc catggagccc ccgacgaacg agaaatccat
      421 gcacgccacc gacatccggt gtccccccac ggtgcctacc gcggcctgca cggcatcgct
      481 gagcccggtc ttcttccgcg cctgtgagag ccgcaccggg tacggcctgg tgtccgtgaa
      541 gccgagcggg tccgtcgaca cgaggttcgt gtccaccttc tcgtacacgc cctcgtcgaa
      601 gatcatccgg aagtacgcct cgcttcccat cgtgaagtgg tggtcgcact tcgggcagac
      661 caggagctgc cgctcgagcc cccggcggtt gaccgtctca ccgcatccgg gacacttgac
      721 ccactggccc tcgggcgtct ggttctgctc ggtgacctcg gtccggatgc ctgccttggt
      781 ccgcttgaac cacgtcatac gggtggggac tgggccgtca gaagggcagt cggtcgaaga
      841 cggagccggc cgccggcctc gccgtgaatt ctcggagata atacggctcg aatcgctcga
      901 cgtccaccgg cccggaaccg gcaccgatcc ggcgcccgag gcggacgacg gcggaggcgg
      961 cgggccgggt cacgggatcc aggacgtgcg gagtgccgag cgccctcagg gcggccgata
     1021 ccaggcggcc accgtcgccg gtcaccacga cgggcccgga cggggaggcg cccagctcct
     1081 gcagggcgtc gcccggcggc ccggcgactg gtccgtgcag cgtcctcgga agaccatccg
     1141 gtccgatgac gtaggaagcg gcgtagactt cggcccgccg ggcccggaag gccgcgacca
     1201 cgatccgtcc gggctccgcc ccggtcgcga gggcgagcgc ttcgagggac ggcaccggaa
     1261 ggaggcctgc cgaggttgcc acgcagatgc ccttggccgt ggataccccg atgcgaagtc
     1321 ccgtgtagga gcccgggccc gcggagaccg ccacggcctg caggtcgtcc gcccggaggc
     1381 ccgccgcgcg aagcgccgct ccgatcatag ggacgaggct ctcggcgtgc ctccgggggc
     1441 ggtccaggct cacggagcag acgacgctcc cgtccacgtc gacggccacg gcgcagacgt
     1501 cggtcgctgt ctctatgccg aggatgacgc tcatgcgcgc cgggcaatcg cccgtcggcc
     1561 ggattgttcc acggcgtgca ggccgccggc ccgccgcggg cgcgtgtggg cttcgtgaat
     1621 ccggccccga accccgccga cacgcgttcc caccgggctc cgggcggtac cgcgcggctt
     1681 gatttccgcg gacccggatg tatctttccg cccctgtttt cgcgtcatat caggtcaaaa
     1741 caacggttcg aactccatga acgtatacga gaccgaccgc atccgcaacg tggctctcgt
     1801 gggtcaccag ggggcgggca agaccatgct cgccgaggcg atgctgcgaa ccagcggagc
     1861 catccagcgg atgggttcga tcgcggaggg gacgacggtc agcgactatc accccagtga
     1921 gaaggagcgt cagatgtccg tgtttgcctc gctgctccag gtggagtggg acggggtcaa
     1981 gatcaacgtg ctcgacacgc ccggctaccc ggacttcgtc ggcgaggtga tcgcctcgct
     2041 ccacgtggcc gatgccgcca tcttcgtgat caacgcggcc gagggcgtgc aggtgggcac
     2101 ggagctcgcg tggcgcactg ccacggagct cggcaagccc tcgatgttcg tcatcaacca
     2161 cctggacaag tcggacacga gcttccgcgc cgtcgtcgag cagatccgcg accgctttgg
     2221 ccgggcggcc agcattgttc agatcccggc cggtgagggc acgcgcgaaa tcgtggacgt
     2281 gctcacgatg aagcaggcgg gcttcgcacc ggacaccggc aagatgaccg agcaggaaat
     2341 cgacgccccg caccgggagg aggccgagcg gctgcacaac gagctcatcg agtccatcgc
     2401 cgagaacgac gaaggcctga tggacctgta tttcgagaag gggagcctca ccgaggacga
     2461 gatgcggaag ggactgcacg aggaactgat ccaccggcag ctcttcccga ttttcgtcac
     2521 gagcgcgacg cagaacatcg gcgtctcgcg gctcatgggc ttcatccgga acgtccttcc
     2581 gaacccggcc gagatgccgc ccgctccgct cgagaacggc ggaaccctgg cgtgcgacgc
     2641 caggggggag cccgtggcct tcgtgttccg gaccatggcc gaggcgcacg tcggcgatta
     2701 ctccttcgtc cgggtcatgt cggggacgct ctcccaggga gatgacctgg tgaatacgca
     2761 ctcgggcgcc tccgagcggc tggggcagct gttctcgatc accgggcacg agcgtgacaa
     2821 cgtggcgcgg ctcgtggccg gcgacatcgg cgcgctggtc aagctcagga acacgcacac
     2881 gaacgatacg ctgctccgca agggatcgaa atcgtcggtg atgcccatcc ggttccccga
     2941 tccgcggtac gccacggccg tcatcgcgac gcgggagggg gaggaggaca agctcgcaca
     3001 ggggctgcac cagctggcca acgaggatcc ctctctcgtg atcacccacg accagcacct
     3061 cagccagctg gttctctcgg gccagggcga gatgcacctg gagatcgcgc ggtttcggct
     3121 caggaaccgt ttcggcgtgg acatcgagta catccgcccg cgggtgccgt accgggagac
     3181 cgtgcagcag caggcgcggt ccaagtaccg tcacaagaag cagaccggtg gagcgggaca
     3241 gttcgcggac atcgcgatgc tcgtcgagcc gctcgtcggc gacttcgcgc ccccgccgga
     3301 catttccgtg cggggcgagc attccgagaa gctggcctgg ggatccacca tccagttcat
     3361 cgatgccatc gtcggtggag tgatcgacat gcgccgcttc ttcggcgcga tccagaaggg
     3421 ggtgatggag gcgctgcaga aaggcccgat cgccggttat cccgtcggtg acatccgggt
     3481 ggtcatctac gacggcggca tgcactccgt cgattcgaac gaggcggcct tcaagacggc
     3541 ggcccgcatg tgcttccggg ccgccttccg cgaggccaac ccgatcctgc tcgagcccat
     3601 ccacaatgtc gaggttctcg tccccgacgc gttcacgggc gacgtcatgg gggacatgaa
     3661 cacgcgccgc gcccgcatcc agggcatcga ctccgagggt ccgttccaga agatcgtggc
     3721 gcaggtaccg gaggccgagc tgcaccggta ttccacctcg ctgcggagca tgacgcaggg
     3781 tcgcggcctg catcgggcgg tgttcagcca ctacgaggcc atgccccgcc acgtacagga
     3841 gaaggtcgtg gccgacgcgg tggcggagca ggaggaatag ccggcgggcg cccgggcagg
     3901 gaggccccgg gttcgtccgg aattccgacg gggcggtcgc ctgcggtggc cgcccctcga
     3961 cgtatggaca tggggtgcgt cgtcagggat cactttgcgc ctcgattttt actacattgg
     4021 gtgtccgcag cctggcgaat gattactaac gtttcgtaaa acgcggccga cgtggacacc
     4081 gatcgcttcc ggacctcgga gcgcgctcgt cgctctatgc ttcgggccgt ctacgaggag
     4141 aaccgccgca tcgcggagga tgacgcgcgg attcgcgagg tccgccgccg ccgatacatc
     4201 gggggcgtgg ggatgctggt ggtggccgtg ggcgcggtcg tcatcatcgc cggcgaatcg
     4261 tcttcgctcc gggctcccga ccggtcggac atggcgtcgc ccacggccat cctcggtgcc
     4321 gatctgccgg tcgtcgacac gtcggacgcg gaggatgagt gggtgcccga ggaggagtac
     4381 agggcgcttc tcgcccagga aaccccgctg gcggcactct tcaacctgag agtccggacg
     4441 atcgtgctgg atcccgggca cgggggggtg gatccgggcg cggtggggcc ggcgggcacc
     4501 ctcgagaagg acatcacgct cgacgtggcg cttcgcctgg cccggcgcct ggaggatcac
     4561 ggctaccgcg ttctgatgac ccggaaggac gaccggacga tctcgctgcg ggagcgggtc
     4621 gagttcgcca acagtcgccc gacggacctg ttcgtgtcga tccatctgaa cgagctcccc
     4681 gtcgattcgg tgaacgtggt ggagacctat ttcttcggcg cgaacaccga gagccgatcg
     4741 cttcgcgtcg ccgagaagga gaattcgggc gcggactaca cggtggcgga cttcaacggg
     4801 atgatccgcc gggtcagcac gaccatgaaa ctccaggaat cccggcaact ggcgcgcgca
     4861 atccagcgca gcgtgtacac gaacaagcgc cagctcgatc tcgaggtggc caactggggg
     4921 gtcaagtcgg gcccgttcgt cgtgctgctg ggctcggagg ctccgagcgt gctcgcggag
     4981 atcggcgtac tgagtcatcc cgaggacgaa cggcgcctgc agagtgccgc gcatcgcgag
     5041 cagctggcga cgttcatgga agagggcatc ctgacctatc tcatcagccg gacccgggac
     5101 gggtcgggct cagaaaccgc acaatagcat ggcgaccaaa aagcagacga gcaagaagaa
     5161 ggcggatgcc tccgccggag aggcgctcta catcggaatc gacctgggca cgtcgcgcag
     5221 cgcgatcgtg gcatcgaacg gcaggcggga gtggatggag agctacgtgg gctggccgcg
     5281 tgatttcgtc tcccggaaga tgctggggcg ccccgtgctc tacgggcgcg aggccatcga
     5341 caaccggctc tcgctgaacc tcgtgcgccc gctcgagcag ggcgtgatcc gcgacgggac
     5401 cgagcgggcg gaagaggccg tgcgccagat ggtgcaccac ctcatcgaga aggtggatcc
     5461 cggaagtgcg acggagatct acgcggcggt gggtgtcccg gccgaggcgc tgcgcgtgaa
     5521 caagctggcg atccgcaacg cggtgaagga gtacgcggac tccctcatgg tggtctcgga
     5581 acccttcgcc gtggcctacg gactgggcac gctcaacaac gcgctcatca tcgacatcgg
     5641 ggccggaacc acggacttct gcatcatgca cggcacgatg cccggcgatg acgatcagcg
     5701 tacgctcacc atcgcgggcg atttcctgga ccagcagctg atggagcgcc tgctcgagcg
     5761 cttccccgag gcctcggtga gtcccaccat ggcccgcggc ttcaaggagg agtacggatt
     5821 cgtgggcaac caccgggacc gcgtgaaggt caaggtgccg atccaggggg cgacgaacgc
     5881 gatcgacatc acttccgacc ttcgaaagac ctgcgagacc ctgcttccgg tgctggcgga
     5941 ggcgacgatg gacctcatcg cccgctacga gccggatttc caggaggagg tcaagcagaa
     6001 tgtgttcctg gccggcggtg gaagccagat caagggcctg gccgaggcgc tgggcgagag
     6061 tctcgcgccc cagggaaact tcaacgtccg ggtgatcgat gatccgcttt tcgccggtgc
     6121 ggagggtgcc ctggcgctcg cgcaggacat gccgcccgag tactgggagg acatgtgaag
     6181 ggtgacggga aacgggcgcc cggggaattc ccggacgccc gtcgttcgcg tggcgcgggg
     6241 cggacttgaa ccgccgacct acgggttatg aatccgtcgc tctgaccacc tgagctaccg
     6301 cgccgcagaa tgtccaaatt accgcacgcg gtcgcctcga tcaacggcgc ccgcgcgccg
     6361 ctctgtgtat cttcacgggt cgtcacagcg ggttgagcct tgagctggga tcgtgtcatc
     6421 ggacagccga gggtcgtgga taccctccgg cgggcggtcg aacgtgaccg ggtcgcacac
     6481 gcctacctgt tccatggtcc ggacggctcg ggcaagcggg ccgccgcgct ggtgcttgcc
     6541 caggcgctcc tctgcgagcg gtccgatggc cggccgtgcg gcacctgttc atcgtgcacg
     6601 cgggcacgcc gcctggtcca tccggatctc cagttccttt tcccgcagcc ggcggacgtc
     6661 tccatggatt cggtacggga gcggctcgac cggatcgccg ccgatccgta cgccaccgtc
     6721 gatttcgtcc ggcgctcgtc gctcgacgat ccagaggccg tgtcgaaccg cgttgtccag
     6781 tactcggtgg cccggatcaa cgaggaagta cgccgcgcga tgagcttccg gtcggtggag
     6841 ggtgggtaca aggtggccat cgtgaccgac gcggaggcgc tgcggaagga ggcggcgaac
     6901 gccttcctga agctgctcga ggagccgacg cccaacacgg tgttcatcct cacgacggac
     6961 cggatggacc gcctgctccc caccatcgtc tcgcgatgcc agcaggtccg gttcgatcgg
     7021 ctcggcgacg aggacgtggc ccgggggctc gccacccggc tgggtcttcc ggacaccgtg
     7081 gcggccacga tcgcccgcat ggccgacggc tcctacagcc gggccctgga gctcgccgcg
     7141 tcagaggagc tcctcgaatc acgcgaggca gccgtggaat tcctgcgcgc cgcctacctg
     7201 gcctggtcgg acgtcgaccg catgtcccaa cgggtcgagg agctggtccg ccggtctcgc
     7261 gagcaccaga aattccagct cggattgatg ctcctgtgga tccgggacgt ggtgctgtac
     7321 cgggagcgtg gtgccgacgc ggcgatcgtg aacgtggacc aggcccgggc gatctccaac
     7381 ttcatcgagg gcgtcccgaa cgccgaccag accgcgatgg tggaactcgt cgaaggagcg
     7441 atcgagctcg tcgagcgcaa cgtgcagtcc aggctcgtgc tcacgactct cgcccagaag
     7501 ctggcccttg cgatgcgggg ccgcggggac ggccggctct acgtccccct gacagacgac
     7561 tgacggcgac gccgcagcgc cttgcgcgaa cccgccgggc gggtatattc gacgtcgggt
     7621 aacggaggtc gccgcgtcat ggcctgtgca tcgggagctt gcggatccgg aggatcggcc
     7681 gggggatcgg ggtgcggttc gggccgctgc ggcaccgatg actgcccctc gacgctcgat
     7741 accgactggc tcgcgggcat cgattcggcc cgtgacgagc cgccgttccc gatcgtggag
     7801 gtggcattca agggcgggcg gcgcgggcat ttccggatca cgggggagac ccggctgcac
     7861 gcgggcgatc cggtgatcgt cgaggcggac cgcggcatcg actacggcac ggtccacctg
     7921 ggcggaccga tggtccggct gcgcctccgc tcgcacggcc aggacgagaa cggcgaatac
     7981 ccggcggttc tccgccgagc gacgcccgag gacaccgagc gctgggaagc gaaccgggag
     8041 cgagacacgg agacgtttct cgtcggccgg cgcacgatcg accggatgaa cctctccatg
     8101 aagctcgtgg acgtcgagtg gcagctcgac ggccgaaagg tcaccttcta tttcaccgcg
     8161 gagcagcggg tggatttccg cgagctggtc cgacgcctgg cccgtcgctt ccgcacgcgc
     8221 gtggagctgc ggcagatcgg cgcgcgggac gaggcggccc gcatcggcgg catcgggtcc
     8281 tgcggccggg agctctgctg ctcgacgtgg ctgcaggagt tccgccccgt ctccacgcag
     8341 gcggccaagt cccagaacct cccgctgaac ccggtccggc tctccgggca gtgcggcagg
     8401 ctcaagtgct gcctgaacta cgagctcgag cagtacctcc aggcgctcgc gcgattcccg
     8461 gcggtggaca cgccgatcac gatcgcgtcc cggatgggag tgatccggaa ggtcgacatc
     8521 ttccgcgaaa ggctctgggt cctgctcgag ggcggggagt ggaccgacat cggactggac
     8581 acggcgagag aggcgctggg catcgagcgc gacccgccgc cggcggagcc gcgggactga
     8641 gtcgggcccg ggctcaggcg atgtcctccg cgtcctcgtc gtcgggagcg atggactgca
     8701 ggaggtcgtc cgggtcgtcg cggacgagcg ccaggatggc cccgtgcgac acgatgaggt
     8761 acgtccggcc ctcccaggcg agctcggtgg cctccttgcg caggaagaag gcatagtccc
     8821 cgggacgtgc ctgcatcggg agatagcgga cggcgtcccg ggacggcgcc cagggctctc
     8881 cttccgaata ttcgggattg ggaatcacgt agcccgggcc gacgcggacg acgcggccgg
     8941 agcgcacctt ctcgcgctcc gcgaccgtcg cgggaagcac gagaccggac tcggtctgcc
     9001 tctcgccctc gtccgggctt atcaggacgc ggtcgccgac caggtggatc tcgctcatgg
     9061 aagtcgctcg ggccggctcc tggccgtctc ggtgagcctc gtaaggaggt cggcgtaccg
     9121 gtccatgatg gtattgcgat cgaagagcgc tgcgcggcgc agcgcgttgt cctgccaatc
     9181 agagaatacg ccaggccggt cgggcaggtt cgcgagcatg gatccgacct cgtgcgccgc
     9241 gtcccagcgg aagacggggc cggcgccggc ctcgtccatc tcccggccga gcgggctgtc
     9301 ggcatcgcag acgacgagca gcggcgtgcc cgacgccatc gacgggagga tcttcgacgg
     9361 tatgaacgat ccgcccgcgc cgtgcgtctc ggtcacgacg aacaggtccg cgtccgagag
     9421 tgcccgcgcg aggccggcct cgtccgtcag gtcgtggaag gtgaaccgcg gatcgccggt
     9481 gccctggacc cagtcccgca cggcctgcgc catgccgccg ccgccctgga tccggaattc
     9541 gaacgccgca tcggagtgcc ggagcgtcga gcagaagccg acgaggtcct gcttggtccc
     9601 gatgtttccg gagtacagga ggcggacggg gcggccgggt gggcggccga gcttgccggg
     9661 gagcgccgcg atctcggcgg cgagcgaggc gtggagccag ttcgggatcg tgtgcagggg
     9721 ctgggagccc cggcgaagcg gctcgagccg ctccgccatc acgggagaga tcgtcgtcca
     9781 cgtgtccgca cggttgaaca gggcacgctg cagcctcgac atccaccggg ccacgcgccc
     9841 cgaccggacg atgcccgagg ccgccgccgc atcgaccgga atgtcctgca cgttgagcca
     9901 gagcggcgct ccggctgccc gtgcggcgac cgctccgaag gctatcgccc cgacgagggg
     9961 acagaacgcc atcaccgcat cgaaacggcc cggatcgggc acgtcccggg cgagcgatgc
    10021 gaaaaacgag gcctcgtaca ggagccgcgg aaacaggcgg ttgggccggg ccgggatgaa
    10081 aaggccgtag cgctcgacga ggaccccgtt ctcacgggcg cgctcgatcc gcagtccgtt
    10141 acgtcctgtc ttgtcgcgcc attcggggta atacgggtag gcgcatcgca cggtgacgtc
    10201 gaagccgcgg gctgcgaggc cctcgcacag gtccgcgaaa agcacgccgc ctcccaggtc
    10261 ggggcgatag acgttcacga tgaccaggag tcggagagcc tcggccacgg ggtgggttgc
    10321 acgtcggttg aagcggggct gcgaaggtac gggatgtgcg ca
//
LOCUS       DBNHQZ010000192         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_464038, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000192 DBNHQZ010000000
VERSION     DBNHQZ010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_464038"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16YU_05185"
     CDS             complement(<1..644)
                     /locus_tag="AC16YU_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter family protein"
                     /protein_id="HIR6064650.1"
                     /translation="MESWTLSAPIGEVFLDIAFIGCCLLAAVLLRRYVRLFQEYLVPA
                     SLIAGFIGLATGPEILGIVRFPIERMGAYVYHLLALTFICVGLHGGGRRHTYGAVNLG
                     FMQVMSMLLQGILGIGIAYLVMLLVDPEVLPASGMLLPLGFAMGPGIAYSIGRSWEAY
                     GFEQAASVGLALAAIGFLAAYSVGVMLVNRGLRRGSAERQSPEGLSRAERTGIR"
     gene            complement(649..1827)
                     /locus_tag="AC16YU_05190"
     CDS             complement(649..1827)
                     /locus_tag="AC16YU_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6064651.1"
                     /translation="MPPSVRHLASEAVLRAAVAPLVHGLWRTRVDPAQIEGVRGPCFL
                     YGNHSNNLDAFIFNMFKPWGEASSGVLTAEYMRGGPVTATLRGIGVLATRKRVAEPHL
                     VGRIMRLLDAGRMVFIYPEGGRRWDGRPRPWIESTAKLFARVGVPVYPVLMHGSYVAW
                     PRWARFPRPARLRIEVLPPVPLDRSLDAASAVARLAAPIAMDENEPPEDTLPRRAFRP
                     ADGFERLLYRDPETGMHGGLFSPDGHRIANAAASIRWRMLPDSRILDERSGAVMLTGD
                     LYDAIRALPLTPGPGGSLLSNRVEFHSGPPYRHAVSHGRARIVMLPDHLEIHSSSGIQ
                     HIPAEEITAVEVERNFKLEIGHSGQVLRFDFVHGGSALQWYDILIGRSALGVYAPHSA
                     "
     gene            1972..2262
                     /locus_tag="AC16YU_05195"
     CDS             1972..2262
                     /locus_tag="AC16YU_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6064652.1"
                     /translation="MTKADIIDRVAAGTGLTKVETEAVVNGFIHTVKDAVLRNERVDL
                     RGFGSFMAQERAARSARNPRTNEPVAVPSAFVPVFKPSREFRRQVDDSLQDS"
     gene            2289..>2977
                     /locus_tag="AC16YU_05200"
     CDS             2289..>2977
                     /locus_tag="AC16YU_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR6064653.1"
                     /translation="MSSTCSSCGAHLEASATVCPLCGTPVDEASSVVPGEPAAPEPEA
                     VRETAPESARSDTVYCNQCGHGNPPGSRFCSSCGAGLHPPTVPASKPPRTSAPAEKSA
                     AGPAAASPAPATGGTSVGTRLGVVFGGAIAIVVLIFLFNAVGNGDVPATAPSAAPGNS
                     APAAASVTTDTPLPDGIADQVEAIQADAEGLQGDARATKLREGVELLLTVGRFDRAGA
                     LQEEVAEFTGT"
BASE COUNT          497 a         1012 c         1058 g          410 t
ORIGIN      
        1 tcccggatgc cggtgcgctc cgcccgcgag aggccttccg gggactgccg ctcggcggag
       61 ccacggcgca gtccgcggtt gacgagcatc acgccgaccg agtacgccgc caggaagccg
      121 atcgccgcga gcgcgaggcc caccgatgcg gcctgctcga atccgtacgc ctcccaggag
      181 cgcccgatgg agtacgcgat cccgggtccc atggcgaatc ccaggggaag cagcatgccc
      241 gacgcgggca ggacctcggg gtccacgagc agcatcacca ggtacgcgat gccgataccc
      301 aggatgccct gcaggagcat cgacatgacc tgcatgaagc ccaggttcac ggccccgtag
      361 gtgtggcgcc ggccgccgcc gtggaggccg acgcagatga aggtgagcgc gagcaggtgg
      421 tacacgtagg cccccatgcg ctcgatcgga aaccggacga tccccaggat ttccgggcct
      481 gtcgcgagcc cgatgaagcc ggcgatcagc gaggcgggga cgaggtattc ctggaacagg
      541 cggacgtagc gtctcagcag gacggcagcc agcaggcagc agccgatgaa cgcgatgtcg
      601 aggaatacct cgccgatcgg ggccgagagg gtccaggatt ccatggcgtc aggcggagtg
      661 gggggcgtag acgcccagcg cggagcgacc gatcaggatg tcgtaccact gcagggcgct
      721 cccgccgtgg acgaaatcga accgcagcac ctgcccggaa tgtccgattt caagcttgaa
      781 gttccgttcc acctcgacgg ctgtaatctc ttcggcggga atgtgttgga ttcccgatga
      841 cgagtggatt tccaggtgat ccggaagcat cacgatgcgt gctctcccgt gggagacggc
      901 gtgccggtag ggcggaccgg aatggaattc gacccggttg gagagcagac ttccgcccgg
      961 acccggggtg agcggaagcg cacggatcgc gtcgtagagg tcacccgtca gcatgacggc
     1021 cccggagcgc tcgtccagaa tccgcgagtc cggcagcatc cgccatcgga tcgacgctgc
     1081 tgcgtttgcg atccggtggc cgtccggcga gaagaggcca ccgtgcatgc ccgtttccgg
     1141 gtcgcggtac aggagccgtt cgaagccgtc ggccggacgg aaggcccggc ggggcagcgt
     1201 gtcctccggg ggctcgttct cgtccatcgc gatgggcgcg gcaagccgtg cgaccgcgga
     1261 agcggcatcg aggctccggt cgagcgggac gggcgggagg acctcgatcc tgagccgggc
     1321 gggccgcgga aaccgcgccc agcggggcca tgcgacgtag gagccgtgca tgaggacggg
     1381 gtagaccggc acgccgaccc gtgcgaagag cttggcggtc gactcgatcc agggtctcgg
     1441 ccgtccatcc cagcggcgcc ccccctcggg gtagatgaac accatgcgcc ccgcgtcgag
     1501 cagccgcatg atcctgccga cgaggtgcgg ctcggccacg cgcttgcgcg tagccaggac
     1561 cccgatgccc ctcaaggtcg ccgtgacagg ccctccccgc atgtattcgg ccgtaaggac
     1621 tcccgacgaa gcctcgcccc acggcttgaa catgttgaaa atgaaagcgt cgaggttgtt
     1681 cgagtggttc ccgtacagga aacatggccc gcggaccccc tcgatctgtg ccgggtccac
     1741 ccgggttcgc cacagaccat gcacgagggg cgccaccgca gcgcgcagta cggcctcgct
     1801 ggcaaggtgg cggacggacg ggggcaagat ccggagctcc ggggtgaggg tggccgaaga
     1861 taggcctcca ccgggggcgc ggccaagcgc ggcatttccc ggcctgcggg gcggatcatg
     1921 gcccgtcgga gggcgtatgc gggcccgaac catgcagacc gattacatag cgtgaccaag
     1981 gcagacatca tagatcgcgt ggccgccggt acgggcctta cgaaggtcga gaccgaggcg
     2041 gtcgtcaacg gcttcatcca tacggtgaag gacgccgtgc tccgcaacga gcgggtggac
     2101 ctgcgcggat tcggctcgtt catggcgcag gagcgcgcgg cccgatcggc ccggaatccg
     2161 cggacgaacg agccggtggc cgtgccgtcg gcgttcgttc ccgtgttcaa gccgtcgcgg
     2221 gagttccgca ggcaggtcga cgacagcctg caggattcct gagccccagc cccgtaccgt
     2281 cccgaaccat gagctcgacc tgttcatcct gcggcgcgca cctggaggcc tcggccacgg
     2341 tgtgcccgct ctgcggtacg cccgtcgacg aggcgtcatc ggtcgtcccg ggggagccgg
     2401 ccgcaccgga acccgaagcg gtccgcgaaa cggccccgga gtccgcgagg tcggacaccg
     2461 tgtactgcaa ccagtgcggg cacggcaatc cgcccggctc gcgcttctgc tcgtcctgcg
     2521 gcgccgggct gcacccgccg acagtcccgg cctccaagcc gccccggacc tccgcacctg
     2581 cggagaagag cgccgccggc cctgcggccg cctcgccggc cccggccacg gggggaacgt
     2641 ccgtcgggac gcgccttggc gtggtcttcg gaggagcgat cgccatcgtg gtgctcatct
     2701 tcctcttcaa tgccgtcgga aacggagacg tgcccgcgac ggcaccctcc gccgctcccg
     2761 ggaactccgc cccggccgct gcctcggtga ccacggacac gcccctcccg gacggcatcg
     2821 ccgaccaggt cgaggcgatc caggcggatg ccgaaggcct ccagggtgac gcgcgggcca
     2881 cgaagctgcg cgagggcgtg gagctcctgc tcacggtcgg ccggttcgat cgggccggcg
     2941 ccctgcagga ggaagtggcc gagttcaccg gtaccga
//
LOCUS       DBNHQZ010000193         3437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1279613, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000193 DBNHQZ010000000
VERSION     DBNHQZ010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1279613"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..438
                     /locus_tag="AC16YU_05205"
     CDS             46..438
                     /locus_tag="AC16YU_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE
                     domain-containing protein"
                     /protein_id="HIR6064654.1"
                     /translation="MKTKTIVGLVLLVAFTSVLFLNFGEQVGGYMSFPEAEESGAKAH
                     VVGVWVAEAPVRYDAQSNVFHFTMKDDHGNVRSVQYANPKPANFEDAEKLVIEGHAAG
                     DHFQAENILVKCPSKYNDGREFEQPTGT"
     gene            complement(442..1851)
                     /gene="mgtE"
                     /locus_tag="AC16YU_05210"
     CDS             complement(442..1851)
                     /gene="mgtE"
                     /locus_tag="AC16YU_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6064655.1"
                     /translation="MEPVSPSEQEAGRTGLVLDPDTELVGDISALVASDQRQMVLNIV
                     ADLHPADIAQLLTHLSRREAKELFGWLSPEVAGTALSELDDDYRAELLEEVPHDRIAE
                     VLDELETDDAADILADLPDHVAQRVLPSLEDAHHVGALLSYDEESAGGIMAVEYVAVR
                     PDWTVARATEEVRRNAEAVENVYVVFVVDEEHRLVGFVSLKRLLLSRASALIADIMNP
                     DVVSAHTDTDQEDVARIMERYDLVSLPVVDAGNRLVGRVTIDDVVDVIREEAEEDIQR
                     MSGVAGGEEPTDSVWRVTRGRLPWLLLGITGAGVAAAVILHFEDTLAAATVLAGFIPI
                     VAATAGNAGIQSSAIAVQGLASGEIWATDFGRRMGREVAVALMNGVAVALFLGVCVLA
                     LGIFLPAEFPDADRLAVTVSASMAVVVVLATTIGASVPILLDKAGIDPAIATGPFITT
                     SNDILGMIVFFLLAEAIYL"
     gene            complement(1899..2684)
                     /gene="rsmA"
                     /locus_tag="AC16YU_05215"
     CDS             complement(1899..2684)
                     /gene="rsmA"
                     /locus_tag="AC16YU_05215"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6064656.1"
                     /translation="MIRPRKRLGQNFLRDPNTARKIVAAVGAPPGEPVVEIGPGTGAL
                     TGLLAERYERLVAIEIDPRAVALLRGEHPGLDVREMDVLHVDWPALAGELGGPVHVVG
                     NLPYYITTPIVFSLLDAGAAVAQALVMMQLEVARRIVAVPRTKEYGILSVVLQRLCRP
                     ELLFEVSRNVFRPRPDVTSAVVRLDFRAGHRDDVDPAWFRTVVRTAFNQRRKTLRNSL
                     SSIGADVPSAWAGARAEELAPHEFVALARYLARPESGAAHPPH"
     gene            complement(2715..>3437)
                     /locus_tag="AC16YU_05220"
     CDS             complement(2715..>3437)
                     /locus_tag="AC16YU_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6064657.1"
                     /translation="ESRPFRPGDDVGRLDAAGTLHRALLRSGPDELSLTEDDLQVFET
                     DHRTSTATVLMIDCSHSMVLYGEDRITPARTTAMALAELILTRYPKDTLDLVAFGTDA
                     WPITIDELPFLEVGPWYTNTLAGLQRARDLLRRRRNRNRQILMITDGKPSCHYEAGRL
                     YRNPYGLDRRIVNRVLDEAVTCRREGITISTFMVARDAYLQNFVRELTAENRGRAYYA
                     RPDDLGTYLFEDFVRNRSRRVR"
BASE COUNT          568 a         1196 c         1168 g          505 t
ORIGIN      
        1 tccggataag gaagacgacc tctgaaccac cctgaacccg gatccatgaa gaccaagacc
       61 atcgtaggcc tggtgctgct cgtcgcattc acctcggtcc tgttcctgaa cttcggcgaa
      121 caggtcggcg ggtacatgag cttccccgag gccgaggaat cgggcgcgaa ggcgcacgtg
      181 gtcggagtct gggtcgccga ggcgccggtc cggtacgacg cccagtcgaa cgtcttccac
      241 ttcacgatga aggacgatca cggcaacgtc cgcagcgtgc agtacgcgaa tccgaagccc
      301 gcgaacttcg aggacgccga gaagctggtc atagagggcc acgccgccgg cgaccacttc
      361 caggcggaga acatcctggt gaagtgcccc tcgaagtaca acgacgggcg cgagttcgag
      421 cagccgaccg gaacctgagt cctacaggta gatcgcttcg gcgagcagga agaacacgat
      481 catccccagg atgtcgttgc tcgtcgtgat gaagggcccg gtggcgatcg cggggtcgat
      541 gcccgccttg tcgagcagga tcggcaccga ggcaccgatg gtcgtggcca ggaccaccac
      601 gaccgccatg gacgccgaga ccgtgaccgc aaggcggtcg gcatccggga actcggcggg
      661 aagaaaaatc cccagcgcga gcacgcacac gcccaggaag agagccactg cgacgccgtt
      721 catcagggcc accgcgacct ccctccccat ccgccgaccg aaatccgtcg cccagatctc
      781 acccgaggcg aggccctgga ccgcgatggc agagctctgg attcccgcat tgccggccgt
      841 ggccgcgacg atcggaatga accccgccag cacggtggcc gccgcgagcg tgtcctcgaa
      901 gtgaaggatc accgcggccg cgacgccggc cccggtgatg cccagcagca gccatggcag
      961 gcggccccgg gtcactcgcc acacggagtc ggtgggctcc tccccgcccg cgacaccgct
     1021 catgcgctgg atgtcctcct cggcctcctc ccggatgacg tccacgacgt cgtcgatcgt
     1081 gacccggccc acgagacggt tcccggcgtc caccacgggc agcgagacca ggtcgtagcg
     1141 ctccatgatg cgggccacgt cctcctggtc cgtgtcggtg tgggccgaga cgacgtccgg
     1201 gttcatgatg tccgcgatga gcgccgaggc ccgcgagagc agcagccgct tcaggctcac
     1261 gaagcccacc aggcggtgct cctcgtccac gacgaagacc acgtacacgt tctccacggc
     1321 ctcggcgttg cgccgcacct cctcggtcgc gcgcgccacg gtccagtccg gccggaccgc
     1381 cacgtactcg acggccatga tgccaccggc cgactcctcg tcgtaggaga gcagcgcgcc
     1441 gacgtggtgg gcgtcctcga gcgagggcaa cacccgctgc gccacgtggt ccgggaggtc
     1501 ggccaggatg tccgccgcgt cgtccgtctc gagttcgtcg agcacctcgg cgatgcggtc
     1561 gtgcggaact tcctccagca gctcggcccg gtagtcgtcg tccagctccg acagcgccgt
     1621 gccggcaacc tcgggggaga gccatccgaa cagctccttc gcctcgcgcc gggaaaggtg
     1681 ggtcagcagc tgggcgatgt cggccgggtg caggtccgcg acgatgttca gcaccatctg
     1741 gcgctggtcg gatgccacca gcgcactgat atcccccacc agctcggtgt cgggatccag
     1801 gaccaggccg gtgcgaccgg cttcctgctc ggaagggctg acgggttcca tggcaactcc
     1861 ggcacgtgcc gattggccgg gtcagcgggc cgccgcgatc agtggggcgg gtgggccgca
     1921 ccggactccg gccgcgcaag atagcgtgcg agggccacaa attcgtgggg agccagctcc
     1981 tcggcccgcg cacccgccca cgccgagggc acgtcggccc cgatcgatga gaggctgttg
     2041 cggagcgtct tgcgccgctg gttgaaggcg gttcggacca cggtgcgaaa ccacgccgga
     2101 tccacgtcgt cccggtggcc ggcacggaaa tccaggcgga cgaccgcgct cgtcacgtcg
     2161 ggcctcggac ggaacacgtt gcgcgacacc tcgaagagca gctcggggcg gcacaggcgc
     2221 tgcagcacga cgctcaggat gccgtactcc ttggtccgcg gaacggccac gatgcgccgg
     2281 gccacctcga gctgcatcat gaccagggcc tgggcgaccg cggctcccgc atcgaggagc
     2341 gagaagacga tgggcgtcgt gatgtagtac ggcaggttgc cgacgacatg caccgggccg
     2401 ccgagctcgc cggccagcgc cggccagtcc acgtggagaa cgtccatttc ccggacgtcc
     2461 agccccgggt gctccccccg gagcagggcc acggccctcg gatcgatttc gatcgcgacc
     2521 agcctctcgt accgctccgc gagcagcccg gtcagcgctc ccgtgccggg accgatctcg
     2581 accaccggct cgccgggagg agcccctacc gcggcgacga tcttccgggc cgtgttgggg
     2641 tccctgagga aattctgccc cagtcgcttg cggggtcgga tcacgtcgat cctccgatct
     2701 ggccggacat cgcctcagcg gacacgccgg gagcggttac ggacgaaatc ctcgaacagg
     2761 taggtgccga ggtcgtccgg acgcgcatag tacgcccttc cccggttttc cgcggtgagc
     2821 tcgcgcacga agttctgcag gtacgcgtcg cgcgcgacca tgaacgtcga gatcgtgatg
     2881 ccctcgcgcc ggcaggtgac cgcctcgtcc agcacccggt tcacgatgcg tcggtccagg
     2941 ccgtacgggt tgcggtacag ccgacccgcc tcgtagtggc agctgggctt gccgtcggtg
     3001 atcatcagga tctgccggtt gcggttccgg cgccgccgga gcaggtcccg cgcgcgctgc
     3061 agcccggcga gggtgttcgt ataccacgga ccgacctcga ggaagggcag ctcgtcgatc
     3121 gtgatcggcc aggcgtccgt tccgaaggct accaggtcga gcgtgtcctt cgggtaccgc
     3181 gtgagaatga gctcggcgag cgccatggcc gtcgtccggg ccggcgtgat ccggtcctcg
     3241 ccgtacagga ccatggagtg cgaacagtcg atcatgagca cggtggccgt gctcgtgcgg
     3301 tgatcggtct cgaagacctg gagatcgtcc tcggtcaggc tcagctcgtc ggggccggag
     3361 cgcaggagcg cccggtgcag cgtgcccgcc gcgtcgaggc ggcccacgtc gtcccccgga
     3421 cggaaggggc gcgactc
//
LOCUS       DBNHQZ010000194         4451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1414566, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000194 DBNHQZ010000000
VERSION     DBNHQZ010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1414566"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="AC16YU_05225"
     CDS             <1..293
                     /locus_tag="AC16YU_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064658.1"
                     /translation="GGAYTVAIDPEHTGPGGSKPNVSATFYRYAGEHADAQDLLRQAS
                     RISMLILLNHLVAGIDAAVSSKLHNDRLDTSLGLAYDPHGAPRPVASVRLRF"
     gene            422..1720
                     /locus_tag="AC16YU_05230"
     CDS             422..1720
                     /locus_tag="AC16YU_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6064659.1"
                     /translation="MPWYRKLHWQIIIGLVLGLGFGILAAASGWSRFTSHWIAPFGTI
                     FINLLKLIAVPLILASLITGVASLSDLKKLSRIGGKTLAIYIGTTVIALVIGLTVVNL
                     MKPGRTVPPEMRERLQQTYQGEADMRTGLAEEAKERGPLQPLVDMVPDNVIGATADNR
                     DMLQVVFFAIFAGIGLLFVPREKAQPLITFFDSLNDLVIKLVELIMVTAPIGVFALMA
                     DTITSIAQDDLGQIVELLAALGYYCIAVVLGLALHMGITYTGMVRFFTPKKLGEFYRA
                     IAPAQLVAFSTSSSGATLPVTMEVVEKDLGVSEEITSFVLPLGATVNMDGTALYQAVA
                     TVFIAQTLGLGLDLAAQLTIVFTAVLASIGTAAVPSAGIVMLVIILEAVGVPSQGIAL
                     IIGVDRILDMLRTVTNITGDAAVALVVATSEGMLSAVDRE"
     gene            complement(1979..2052)
                     /locus_tag="AC16YU_05235"
     tRNA            complement(1979..2052)
                     /locus_tag="AC16YU_05235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2016..2018),aa:Arg,seq:ccg)
     gene            2191..3024
                     /locus_tag="AC16YU_05240"
     CDS             2191..3024
                     /locus_tag="AC16YU_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6064660.1"
                     /translation="MMTDTALFQRFKADPERGYHELLDQYTPVILRMIRRFVRDPDEV
                     METYTAICERLRTNDYSALRRFRENSALTPWLSVVVANACRDRFRKFRTVSAPESVLS
                     QLTDRERLVFRYYYQERLLHGDIAIVLQSRHRIRCTEYDVARDLGRIDDLLSVAKRWH
                     LLAAIRANLPVLAFEDLTDGSLEHSELAVNPDALSDGPDEKLLQRLNDAVKTLDPDDQ
                     LLLLMRFEQEMRANDIARAMRFENPKYVYTRLRTVVGKLRRLMGVEVPVGKGNGKAVR
                     D"
     gene            3111..4103
                     /locus_tag="AC16YU_05245"
     CDS             3111..4103
                     /locus_tag="AC16YU_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR6064661.1"
                     /translation="MPPDTLPTDLPPAAARRFPSLPDVGPLDGPAERAGFAPGLTVGL
                     GLVAGFLLFQLLSLAVALGLIVAAGGLEDPAGMDIGALIDRFAREFLVANTVGQVLGL
                     GVFAWILARLHTRRAGRFLRVRASDARFAALSVVGLLLLVPVVQWLGMWSDSWPWPEW
                     VRSFEATQLDLIEKVLTQNLGFVFTLFTMAVTPAVCEELFFRGYIQRQAERMFGGWIW
                     AVVFSGVVFGLYHVRPTQALPLSLLGVYLAYVTWRSGSLVPALLVHFANNGLAVVLGA
                     IVSARPDLQVADLEKMAIPAYIVFPAAVLFAGLAWVLHTRFRRREVDFENHHAE"
     gene            4107..>4451
                     /locus_tag="AC16YU_05250"
     CDS             4107..>4451
                     /locus_tag="AC16YU_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR6064662.1"
                     /translation="MDQETYQGWVSVFQSGTDYEADLVRDRLDDSGIPAVVLTHRDHA
                     FNLTVGDMAAVRVLVPPERVKEALALLGSEPFTYEELSRIAMAADPDAPSAHDPDREA
                     MLDSGIESIHLRK"
BASE COUNT          676 a         1519 c         1500 g          756 t
ORIGIN      
        1 aaggcggcgc gtacacggtg gcgatcgacc cggagcatac cggcccgggc ggctccaagc
       61 ccaacgtctc ggccacgttc taccggtacg cgggcgagca tgccgacgcg caggacctcc
      121 tgcggcaggc cagccggatc tcgatgctga tcctcctgaa ccacctggtc gccgggatcg
      181 acgcggcggt ctcttccaag ctgcacaacg accggctgga cacgagcctg ggcctggcct
      241 acgatcctca tggggcaccg cggccggtgg cgtccgtccg tctccggttc tgagccggct
      301 gtgggaatgc ccgggccgtg ggcgccgggt ccttgatttt gggtcgccgc gggcgtgatc
      361 ttggcgcgcg cccgtctccg gctcccggtc catggagcct ccacgatccc gccccacgcc
      421 catgccctgg taccggaagc tccactggca gatcatcatc ggcctcgtgc tcggactggg
      481 gttcggcatc ctggccgccg cgtcgggctg gagccggttc acgtcgcact ggatcgcgcc
      541 gttcgggacg atcttcatca acctgctgaa gctcatcgcg gtcccgctca tcctggcgtc
      601 gctcatcacg ggcgtggcct cgctgtccga cctcaagaag ctctcgcgga taggcggcaa
      661 gaccctggcg atctatatcg ggacgaccgt catcgcgctc gtcatagggc tcacggtcgt
      721 caacctgatg aagcccgggc ggacggtccc gcccgagatg cgggaacgcc tgcagcagac
      781 gtaccagggc gaggccgaca tgcggacggg cctggccgag gaagccaagg agcgcggacc
      841 gctgcagccg ctcgtggaca tggtgcccga caacgtgatc ggcgcgacgg cggacaacag
      901 ggacatgctc caggtggtct tcttcgcgat tttcgcggga atcgggctcc tgttcgtgcc
      961 gcgggagaag gcccagccgc tcatcacgtt cttcgacagc ctgaacgacc tggtcatcaa
     1021 gctcgtcgag ctcatcatgg tgacggctcc catcggggtg ttcgccctga tggccgacac
     1081 gatcacgtcc atcgcgcagg acgacctggg gcagatcgtg gagcttctcg cggcgctggg
     1141 gtactactgc atcgcggtgg tcctgggcct cgcgctgcac atgggcatca cgtacaccgg
     1201 catggttcgg ttcttcacgc cgaagaagct gggcgagttc taccgggcca tcgcccccgc
     1261 gcagctcgtc gccttttcca cgtcgtcgtc gggcgccacg ctgcccgtca ccatggaggt
     1321 cgtcgagaag gacctggggg tgtccgagga gatcacgtcg ttcgtgctcc cgctcggggc
     1381 cacggtcaac atggacggaa ccgccctgta ccaggccgtc gcgacggtat tcatcgccca
     1441 gacgctcggg ctcggactgg acctggccgc gcagctcacc atcgtgttca cggcggtcct
     1501 cgcgtccatc gggacggcgg ccgtaccgag cgccggcatc gtgatgctcg tcatcatcct
     1561 cgaggcggtc ggcgtgccca gtcagggcat cgccctgatc atcggggtgg accgcatcct
     1621 ggacatgctc cggacggtca cgaacatcac gggcgatgcc gcggtggccc tggtcgtggc
     1681 gacgagcgag gggatgctga gcgcggtcga tcgggagtga tcgcgcccgg accggaaccg
     1741 ggcgtcccgg cgcgataccc gaattgagac gacggcctgc ccgtccgcag gggcaaatgc
     1801 gcccgtacgg gccgtgggag cggttccaga agtgaatccg ggcggccgcc gccagccggg
     1861 tgagattggc accaagttgg acgaaggtcc gggggcacct tgacacccac cgggccggat
     1921 ccgccgcctt cacgggcagc tacgaaaagg cccgacggat ctgcatccga cgggcctgcg
     1981 cgcttggagg gattcgaacc cccgaccttt ggaaccggaa tccaacgctc tatccagctg
     2041 agctacaagc gcgggacctg aaagatagcg gagccgccga agtggggtca aggcgcaatc
     2101 ccggatgccc gacaagattg tgctaatacc aggacccact tgccgaccca tttgaccatc
     2161 cgtggcctgc ggccgcgcgg agccccgccc atgatgaccg ataccgcgct gtttcagcga
     2221 ttcaaggccg atccggagcg aggctaccac gagctgctcg accagtacac cccggtcatc
     2281 cttcgcatga tccggcgttt cgtccgggat cccgacgagg tcatggagac ctataccgcg
     2341 atctgcgagc ggctgcgtac caacgactat tccgcgctgc ggcgcttccg ggagaacagc
     2401 gcccttacgc cgtggctctc ggtcgtggtc gcgaacgcgt gccgggaccg gttccgcaag
     2461 ttccgcacgg tatccgcgcc ggagtcggtc ctctcgcagc tcactgaccg ggagcggctg
     2521 gtcttccggt actactacca ggagcggctc ctgcacggcg acatcgcgat cgtgctccag
     2581 agccggcatc gcatccggtg cacggagtac gacgtcgcgc gagacctggg ccgcatcgac
     2641 gacctgctgt cggtggccaa gcggtggcac ctcctggccg cgatccgggc caatctcccg
     2701 gtcctcgcgt tcgaggacct gaccgacggg agcctcgagc acagcgagct cgcggtgaat
     2761 cccgatgccc tgtccgacgg gcccgacgag aagctgctgc agcggctcaa cgacgccgtg
     2821 aagacgctgg accccgacga ccagctcctg ctcctgatgc gcttcgagca ggagatgcgg
     2881 gcgaacgaca tcgcgcgagc gatgcggttc gagaacccca agtacgtcta cacgcggctt
     2941 cggaccgtcg tgggcaagct acgccgcctg atgggcgtcg aggtcccggt cggaaaaggc
     3001 aacggaaagg ccgtccggga ctgatccggg gatccgccgg ccgccgatcc cgtatgttgc
     3061 gcgccgccgg gccgtgaccg gcgaaaacag catcgcctat cgccccgatc gtgcccccgg
     3121 acaccctgcc gaccgacctg ccgccggccg ctgcgcggcg gtttccgtcg cttcccgacg
     3181 tgggccctct cgacggtccc gccgagcgcg ccggattcgc cccgggactg accgtggggc
     3241 tcgggctcgt tgcgggcttc ctgctgttcc agctcctgag cctggccgtc gcgctcgggc
     3301 tcatcgtggc cgcgggcggg ctggaggatc cggcgggaat ggacatcggc gcgctcatcg
     3361 accggttcgc cagggaattc ctggtcgcga acacggtggg gcaggtgctg ggcctgggcg
     3421 tgttcgcctg gatcctggcc cggctgcaca cccgccgcgc cggtcgtttc ctccgggttc
     3481 gtgcgagtga cgcccgcttc gcggcgctgt ccgtcgtggg gctgctgctc ctggtgcccg
     3541 tcgtccagtg gctcggcatg tggagcgaca gctggccctg gccggaatgg gtccggtcgt
     3601 tcgaggcgac gcagctggat ctcatcgaga aggtgctcac ccagaacctg ggcttcgtct
     3661 tcacgctctt caccatggcc gtgacgcccg ccgtgtgcga ggagctcttc ttccgcgggt
     3721 acatccagcg gcaggccgag cgcatgttcg gcgggtggat ctgggcggtg gtgttctcgg
     3781 gcgtcgtctt cggcctgtac cacgtccggc cgacccaggc cctgcccctg tccctgctcg
     3841 gcgtgtacct ggcgtacgtg acctggcgat ccggcagcct ggtacccgcg cttctcgtgc
     3901 acttcgcgaa caacgggctc gccgtcgtac tcggggccat tgtctcggcg cgacccgacc
     3961 tgcaggtcgc cgatctcgaa aagatggcca ttccggccta tattgtattc ccggcggcgg
     4021 ttctcttcgc cggcctggcg tgggtgctgc acacgcggtt ccggcgccgg gaggtggatt
     4081 tcgagaacca tcacgcggag taggccatgg accaggaaac atatcagggt tgggtgtccg
     4141 tctttcagag cgggacggac tacgaagccg acctggtgcg ggaccggctc gacgattccg
     4201 ggattcccgc ggtcgtcctc acgcatcgcg accacgcctt caacctgacg gtcggggaca
     4261 tggctgcggt tcgcgtgctg gtgccgcccg aacgggtgaa ggaggccctc gcactgctcg
     4321 ggagcgagcc cttcacctac gaggagcttt cgcggatcgc gatggcggcc gaccccgacg
     4381 caccttccgc gcacgacccg gaccgggagg cgatgctgga ttcgggcatc gaatcgatcc
     4441 acctccgaaa g
//
LOCUS       DBNHQZ010000195         2539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_736686, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000195 DBNHQZ010000000
VERSION     DBNHQZ010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_736686"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2539)
                     /gene="acnA"
                     /locus_tag="AC16YU_05255"
     CDS             complement(<1..>2539)
                     /gene="acnA"
                     /locus_tag="AC16YU_05255"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6064663.1"
                     /translation="DGYVVTEDDVKALAAWNAAAPAKVEIPFMPARVVLQDFTGVPCV
                     VDLAAMRSAMARLGGDPDSINPRVPVDLVIDHSVQVDEYGSAAALFINAEKEFERNRE
                     RYEFLRWGRQAFDNFRVVPPASGICHQVNLEYLARCVWSRPEADGSTVYYPDSLVGTD
                     SHTTMIDALGVVGWGVGGIEAEAVMLGQPIYMLMPEVIGFRLTGRLPEGATATDLVLT
                     ITQTLRSYGVVGKFVEFFGPGISGMSIPDRATIANMSPEYGATMGFFPIDDESLAYLT
                     RTGRDPALVQTVERYAKLQGLFRTDDMTEPEFRDTLELDLGSVRPSLAGPKRPQDRVN
                     LGEVGQVFRASLSAPVGPKGFGLPADAVDRRADFVDASGAKHTLEMGDVVIAAITSCT
                     NTSNPSVMLGAGLVAKKAVERGLRVKSHVKTSLAPGSKVVTEYLDEAGLRPYLDQLGF
                     NLVGYGCTTCIGNSGPLPAPVSEAVRTGDLVVAGVLSGNRNFEGRIHQQVQANFLASP
                     PLVVAYALAGTVDIDLTHDPIGHDGAGRPVYLRDIWPTNREIREAVNAAVKPEQFRRE
                     YDGIEDSNEEWNAIEIPEGSIYEWNLKSTYVQEPPFFQGLSRETPPVETIRGARVLIK
                     VGDSTTTDHISPAGSIAPDSPAAGYLLSNDVKYLDFNSYGSRRGNHEVMMRGTFANIR
                     IKNHILKGVEGGVTRYFPTGETMAIYDAAMKYRDDGTPLVVLAGKDYGMGSSRDWAAK
                     GTLLLGVRAVIAESFERIHRSNLIGMGVLPLQYADGQTAESLGLDGTETYSIPVGEDL
                     AAGQAVTVTAMREDGTRFEFRTLCRIDTPVEVDYYRSGGILHFVLRE"
BASE COUNT          379 a          866 c          876 g          418 t
ORIGIN      
        1 aactcccgca gcacgaagtg caggatgccg ccgctgcggt agtagtccac ctcgaccggg
       61 gtgtcgatgc ggcagagggt ccgaaactcg aaccgcgtcc cgtcctcgcg catggccgtg
      121 accgtcaccg cctgcccggc cgcgaggtcc tcgcccacgg ggatcgagta cgtctcggtg
      181 ccgtccaggc cgagcgactc ggccgtctgt ccgtccgcat actgcagcgg cagcacgccc
      241 atgccgatca ggttggaccg gtggatgcgc tcgaagctct cggcgatgac ggcccggacg
      301 ccaaggagca gggtgccctt ggccgcccag tcgcgcgagg agcccatccc gtagtccttc
      361 cccgcgagca ccacgagcgg cgtgccgtcg tcgcggtact tcatggccgc gtcgtagatc
      421 gccatggtct ccccggtcgg gaagtaccgc gtgacgcccc cctccacgcc cttcaggatg
      481 tggttcttga tgcggatgtt ggcgaaggtg ccgcgcatca tcacctcgtg gttcccacgc
      541 cgcgagccgt acgagttgaa gtccaggtac ttcacgtcgt tcgacaggag gtagccggcc
      601 gcggggctgt cgggggcgat ggacccggcc ggcgagatgt ggtccgtggt cgtcgagtcg
      661 ccgaccttga tcagcacgcg ggccccccgg atcgtttcga ccggcggggt ctcgcggctc
      721 agcccctgga agaacggcgg ctcctgcacg taggtggact tcaggttcca ctcgtagatc
      781 gagccctcgg ggatctcgat cgcgttccac tcctcgttcg agtcctcgat gccgtcgtac
      841 tccctgcgga actgctccgg cttcacggcg gcgttcacgg cctcccgtat ctcccggttc
      901 gtcggccaga tgtcgcgcag gtacacgggt cggccggcgc cgtcatggcc gatcggatcg
      961 tgggtcaggt cgatgtccac ggtgccggcc agcgcgtacg cgacgacgag cggcggcgag
     1021 gccaggaagt tggcctggac ctgctggtgg atgcggccct cgaaattgcg gttgcccgag
     1081 agcacgccgg cgacgacgag gtcgccggtc cggacggctt ccgagacggg cgcgggcagc
     1141 gggccggagt tgcctatgca cgtcgtgcat ccgtagccga ccaggttgaa tccgagctgg
     1201 tccaggtagg gcctgaggcc ggcctcgtcc aggtattcgg tgaccacttt cgagcccggc
     1261 gccagcgacg tcttcacgtg cgatttcacg cgcaggccgc gctcgacggc cttcttcgcg
     1321 accaggcccg cgcccagcat gaccgacggg ttcgacgtgt tcgtgcagct cgtgatggcc
     1381 gcgatcacca cgtcgcccat ctcgagcgtg tgcttcgccc ccgatgcgtc gacgaagtcc
     1441 gcgcggcggt cgaccgcgtc tgccggcagg ccgaatccct tcgggccgac gggcgccgaa
     1501 agagaggcgc ggaagacctg gccgacctct ccgaggttga cccggtcctg ggggcgcttc
     1561 gggcccgcga ggctggggcg gacggagccg aggtccagct cgagcgtgtc gcggaattcc
     1621 ggctcggtca tgtcgtcggt ccggaaaagg ccctgaagct tcgcgtagcg ctccacggtc
     1681 tgcacgaggg cgggatcacg gcccgtgcgg gtcaggtagg ccagggactc gtcgtcgatc
     1741 gggaagaagc ccatcgtcgc tccgtattcc ggcgacatgt tcgcgatcgt cgcccggtcc
     1801 gggatcgaca tgccggatat cccgggcccg aagaattcca cgaatttccc gaccacgccg
     1861 tacgagcgca gcgtctgggt gatggtgagc accaggtcgg tcgcggtggc gccctcgggc
     1921 aggcggcccg tcaggcggaa cccgatgacc tcgggcatga gcatgtagat gggctgcccc
     1981 agcatcacgg cctccgcctc gatgcccccg acgccccagc ccacgacacc cagcgcgtcg
     2041 atcatggtcg tgtggctgtc ggtcccgacc agggagtcgg gatagtacac cgtcgatccg
     2101 tcggcctccg gccggctcca cacgcagcgg gccaggtact cgaggttcac ctggtggcag
     2161 atgcccgagg cgggggggac gacgcggaaa ttgtcgaacg cctgccggcc ccagcgcagg
     2221 aactcgtagc gctcgcggtt gcgctcgaac tccttctccg cgttgatgaa gagcgcggcc
     2281 gcagagccgt actcgtccac ctgcaccgag tggtcgatca ccaggtccac ggggacgcgg
     2341 ggattgatcg agtcgggatc gcctccgagc cgagccatcg ccgagcgcat ggcggccagg
     2401 tcgacgacgc atggcacgcc ggtgaaatcc tgcagcacga cgcgggccgg cataaatggg
     2461 atttcgacct tggccggcgc cgctgcattc cacgccgcca gggccttcac gtcgtcttcg
     2521 gtgacgacgt acccgtcgc
//
LOCUS       DBNHQZ010000196         3010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3581690, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000196 DBNHQZ010000000
VERSION     DBNHQZ010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3581690"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC16YU_05260"
     CDS             complement(<1..650)
                     /locus_tag="AC16YU_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6064664.1"
                     /translation="MKVSVTTISVAELDVDLLLIPRTREQADGTMADLASGFGGSVRR
                     SAPDFSGEPDSSLLLYPETGSARRVAFVGLGASERVDAEALRVAAARGAAMARSAKAE
                     TVGISLDAWDLDAEEAAQALVEGFILGAYRFDRYRTDARDEGSLQRLVLQATDKDKAV
                     RRGAERGRVVAECVCSARDLVNTSPDEKTPILLARAMERSAKKYGYVASVWDRALI"
     gene            complement(647..1735)
                     /locus_tag="AC16YU_05265"
     CDS             complement(647..1735)
                     /locus_tag="AC16YU_05265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR6064665.1"
                     /translation="MPDTLVRRLLDARRIVLTTHERPDGDAIGSEIAMALYLRGRGKE
                     VHVINADPAPHTVEWLPGADVLTVFDGSIAQREAIAAADVILVIDTNAEHRLGRVGPP
                     VRSSGAHTVVVDHHTAPEPWFDSAYRRESASSTGELVYEIIRADDPGLIDAPIATALY
                     VAIMTDTGSFRFSNVSPAVHRVVADILERGALDPALIHAEVYDKRTPAGLRLLSRVLQ
                     TLTLAHDGQVAWMVVTPRMLQETGSSIEETEGFVNWGLSIEGVRASLLFTETERGTKV
                     SFRSKRDFHVHTWAQALGGGGHPNASGAFVRSPLDETIDRVVAQAPRYLDLEPAGAEP
                     DPASDRVSSEDEAYLAALMNLQNREERT"
     gene            complement(1843..2496)
                     /gene="pdxH"
                     /locus_tag="AC16YU_05270"
     CDS             complement(1843..2496)
                     /gene="pdxH"
                     /locus_tag="AC16YU_05270"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011769857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR6064666.1"
                     /translation="MNLLKSLRMMFTLGHGLRVGIPEAAESDDPVRLFGEWYGAAERS
                     GLALPEAMTLATATPDGRPSARMVLLKGYGADGFVFYTNYGSRKAAELDANPRAALVF
                     HWAVLQRQVRIEGAVERVDEATSGAYFASRGRGSRIGAWASRQSRPLARREDLEARFR
                     EMDERFSGSDVPLPPFWGGYRLRPESIEFWQGRPDRLHDRLRFDRSGDGWNPVRLQP"
     gene            complement(2515..>3010)
                     /locus_tag="AC16YU_05275"
     CDS             complement(2515..>3010)
                     /locus_tag="AC16YU_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6064667.1"
                     /translation="LNRWEPWNGIRTVRAGLVDAVQVIYNIFDQAPEDELFPACREMD
                     VAVIARVPFDEGTLTGTLTRDSTWPKGDWRNLYFVPENLVPSVERAERLRPDLPDGIS
                     MPEAALRFILAERTVSTIIPGMRKLRHVDANCATSEAGPLPADVQERLRRHRWDRQPT
                     AWSL"
BASE COUNT          442 a         1079 c         1054 g          435 t
ORIGIN      
        1 tcgatcaggg cgcggtccca gaccgaggcc acgtacccgt acttcttggc cgagcgctcc
       61 atggcgcggg ccagcaggat cggcgtcttc tcgtcgggcg acgtgttcac gaggtcgcgc
      121 gcgctgcaga cgcattccgc aaccacgcgc ccgcgctccg cgccgcggcg caccgccttg
      181 tccttgtccg tggcctgcag cacgagccgc tgcagcgagc cctcgtcccg ggcatcggtg
      241 cggtaccggt cgaagcggta cgcgcccagg atgaagccct ccaccagggc ctgcgccgcc
      301 tcctcggcgt ccaggtccca ggcgtcgagc gagatgccga cggtttccgc cttggcggag
      361 cgcgccatcg cggccccgcg ggccgccgcc acgcgaagcg cctcggcatc cacgcgctcc
      421 gacgccccga ggcccacgaa ggccacccgg cgcgccgacc ccgtctcggg gtacagcagc
      481 agcgaactgt cgggctcccc ggaaaaatcg ggggcggaac ggcggaccga gccgccgaaa
      541 ccggacgcca ggtcggccat cgtgccgtcc gcctgttcgc gggtacgggg aatgagcagg
      601 aggtctacgt cgagctcggc gaccgatatg gtcgtgacgg atactttcat gttcgctctt
      661 cgcggttctg tagattcatc agggcggcca ggtaggcctc gtcttccgac gaaacccggt
      721 ccgacgccgg gtcgggttcc gcgccggccg gctccaggtc caggtaccgg ggggcctggg
      781 ccacgacccg gtcgatggtc tcgtcgagag gactgcggac gaaggcgccc gaggcgttcg
      841 gatgtccgcc gccgcccagc gcctgcgccc acgtgtgcac gtggaaatcg cgcttggaac
      901 ggaagctgac cttcgtgccg cgttcggttt ccgtgaacag cagcgaggca cggacgccct
      961 cgatcgagag cccccagttc acgaatccct cggtctcctc gatggacgat ccggtctcct
     1021 gaagcatgcg cggcgtgacc accatccacg cgacctgccc gtcgtgcgcg agggtgaggg
     1081 tctggagcac gcggctcagc aggcgaaggc ccgcgggggt gcgcttgtcg tacacctcgg
     1141 cgtggatcag ggcaggatcg agcgcgccgc gctcgaggat gtcggcgacg acgcggtgca
     1201 ccgcggggga cacgttcgaa aaacggaacg agcccgtgtc cgtcatgatc gccacgtaga
     1261 gcgcggtcgc gatcggcgca tcgatcaggc ccggatcgtc ggcccggatg atctcgtaga
     1321 cgagctcgcc cgtggacgag gcgctctccc tgcggtacgc cgaatcgaac cagggctcgg
     1381 gcgccgtgtg gtggtccacg acgacggtgt gcgcgccgct cgagcgcacg ggcggcccga
     1441 cgcgtcccag gcggtgctcg gcgttcgtgt cgatcacgag gatgacgtcg gccgcggcga
     1501 tggcctcccg ctgcgcgatc gagccgtcga acacggtgag cacgtcggcc ccgggcagcc
     1561 actccaccgt atgcggggcc gggtccgcgt tgatgacgtg gacctccttc ccgcggccgc
     1621 gcaggtacag cgccatcgcg atttcggagc cgatggcgtc accgtccgga cgctcgtgcg
     1681 tggtgagcac gatgcgccgg gcgtcgagca ggcgtcgtac gagcgtgtcc ggcatcgggg
     1741 gtggcgatta cgaaaaacag agccgcagtt aaacgcgaac ccccttccgt tgttcgtcgc
     1801 cgggcgcccc ggggatcccg ccccgccccg gatccggcgc tcctacggct ggagcctcac
     1861 cggattccag ccgtcacccg accggtcgaa ccgcagccgg tcgtgcaggc ggtccggccg
     1921 cccctgccag aactcgatcg attccggccg gagcctgtac ccgccccaga agggcggcag
     1981 cggaacgtcc gaacccgaga agcgctcgtc catctcccgg aaccgcgctt ccaggtcctc
     2041 ccgacgcgcg aggggccggc tctgccggga cgcccaggcc ccgatccggc ttccgcggcc
     2101 ccgcgaggcg aagtacgccc cggacgtcgc ctcgtcgacg cgctcgacgg cgccctcgat
     2161 ccgcacctgc cgctgcagca ccgcccagtg gaaaacgagc gccgcgcgcg gattcgcgtc
     2221 gagctcggcc gccttgcggc ttccgtagtt cgtgtagaag acgaagccgt ccgcgccgta
     2281 gcccttcagc agcaccatcc tcgccgacgg ccgcccgtcg ggcgtggccg tggccagcgt
     2341 catggcctcg ggcagggcga gcccggagcg ctccgcagcc ccgtaccact cgccgaagag
     2401 ccggaccgga tcgtccgact ccgcggcctc cgggatgccc acgcgcagcc cgtggcccag
     2461 cgtgaacatc atgcgcaggg acttgagcag gttcatgagc gggtcttcgg cggatcagag
     2521 agaccaggcc gtcggctgac ggtcccagcg gtgccggcgg agccgctcct gcacatccgc
     2581 ggggagcggt ccggcctcgg aggtcgcgca gttcgcgtcc acgtgccgca gcttgcgcat
     2641 gcccggaatg atcgtcgaga cggtccgctc ggcgaggatg aagcgcaggg cggcctcggg
     2701 catggagatc ccgtcgggca ggtcggggcg cagccgttcg gccctttcaa cggacggaac
     2761 caggttttcc ggcacgaaat acaggttgcg ccagtcgccc ttgggccacg tcgagtcccg
     2821 ggtcagtgtg cccgtgagcg ttccctcgtc gaagggcacg cgcgcgatga cggccacgtc
     2881 catctcgcgg cacgccggga agagctcgtc ctcgggcgcc tggtcgaaga tgttgtagat
     2941 gacctgcacg gcgtccacga ggcccgcgcg gacggtccgg atgccgttcc agggctccca
     3001 ccggttcagg
//
LOCUS       DBNHQZ010000197         2709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_194856, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000197 DBNHQZ010000000
VERSION     DBNHQZ010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_194856"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="AC16YU_05280"
     CDS             <1..794
                     /locus_tag="AC16YU_05280"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR6064668.1"
                     /translation="PAIYLEPSDVEVEVGSVAVLAVRATGFPVEYTWLRDGVPVEEAT
                     GPGMSVVPDSMEEASTWQVVAHNGLGGDTSRTATVTVLPFAGPTVPRAAAFRAWMPIG
                     QLATGPSMPEPDDLSARWALRQDDEGLHGRVEVADPVLRNGDGRPYHNDGVEIYLDPD
                     NAKALDYEPDDVQIRIVRDGPVTADRGTLPDGFQAVQREVPGGWVVDFTVPIEAFADA
                     GRFVGIDLHVVDNDGDRREHKIAWAASDDGAYRSPAAFGTVRLGN"
     gene            complement(825..1799)
                     /locus_tag="AC16YU_05285"
     CDS             complement(825..1799)
                     /locus_tag="AC16YU_05285"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6064669.1"
                     /translation="MKAFITGATGFIGTRVAERMARAGHSMRCLVRPASDATRIEVLG
                     AEVVRGDVVDREAVLRGMEGCDHVIHLAAAYDFWLPDPAVYRDVNVTGTRNVMECALQ
                     TGVLKVTHVSSIVVYGTPADDPVTESSDFGRRRWSEYARTKFEGDQIAWDLHSRGLPL
                     VVLYPGAVLGPGDTKATGRYIESLVHHRTPATVFDRAVFPWVHVDDVAEAVVRATEKP
                     DNSGERYIVSAESLTFGEINHMVSEISGEPLPKPHLPDRLAILMAGGLTRWADLVKRP
                     PPWGMAIDQMQTMGHGARADGHKAERELGFTYTSVRTALEQEVAFARN"
     gene            complement(2071..>2709)
                     /locus_tag="AC16YU_05290"
     CDS             complement(2071..>2709)
                     /locus_tag="AC16YU_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064670.1"
                     /translation="SAAMTLADWYDADAHFAWAGVGGEYVPVSLRVRRTFQRSEVSAT
                     FWSDAAFNLLVRAAEENDRRALFILGSGLDSQFGGRPYPNEVRRDDYLVRMLLSDGPD
                     PELDGFVFRRLGSRNPILAESVRREADRNGLLWPTLQSARDAAAAGNWDRWMEVWAGA
                     LERDVPGARVGWIRERSRLLEQVDAGNGASIALLERLRAFDRAHPAVGLFGV"
BASE COUNT          404 a          983 c          923 g          399 t
ORIGIN      
        1 cgccggccat ctacctcgag ccctcggacg tcgaggtcga ggtcggctcg gtggcggtcc
       61 tcgcggtccg ggcgaccgga ttccccgtcg agtacacgtg gctccgcgac ggcgtcccgg
      121 tcgaggaggc tacgggaccg ggcatgtccg tcgtgccgga ttccatggag gaggcttcga
      181 cctggcaggt cgtcgcgcac aacgggctgg gcggcgatac cagccgcacg gccacggtca
      241 ccgtcctgcc gttcgccggg ccgacggtac cgcgcgcggc tgcgttcagg gcctggatgc
      301 cgatcgggca actggccacc ggcccgtcca tgcccgagcc cgacgacctg tcggcgcggt
      361 gggcgctgcg ccaggacgac gaaggcctgc acggccgcgt ggaggtggcg gaccccgtcc
      421 tgcgcaacgg cgacggccgg ccgtaccaca acgacggcgt cgagatctac ctggacccgg
      481 acaacgccaa ggcgctcgac tacgagccgg acgacgtgca gatccggatc gtccgggacg
      541 gacccgtcac ggcggaccgc ggcacgcttc cggatggctt ccaggcggtg cagcgcgagg
      601 tcccgggcgg ctgggtggtc gacttcacgg tgccgatcga ggcgttcgcg gacgcgggac
      661 ggttcgtcgg catcgacctc cacgtggtcg acaacgacgg cgaccggcgt gagcacaaga
      721 tcgcgtgggc ggcctcggac gacggggcgt accgctcgcc cgcggcgttc gggacggtga
      781 ggctcggcaa ctgaacccac cggcagcgtg cgcaccccga ccgctcagtt cctcgcgaac
      841 gcgacctcct gctcgagcgc ggtccggacg ctcgtatagg tgaatccgag ctcccgctcc
      901 gccttgtgcc cgtcggcccg cgctccgtgg cccatggtct gcatctggtc gatcgccatg
      961 ccccagggcg gtggcctctt gaccaggtcg gcccaccgcg tgagcccgcc ggccatgagg
     1021 atcgccaggc gatccggaag atgcggcttc ggcaggggct cccccgagat ctcggagacc
     1081 atgtggttga tttccccgaa ggtcaggctc tcggccgaga cgatgtaccg ctcccccgag
     1141 ttgtccggct tctcggtggc gcgcacgacg gcctccgcca cgtcgtccac gtgcacccag
     1201 ggaaagaccg cacggtcgaa aaccgtggcc ggcgtgcggt gatgaacgag gctctcgatg
     1261 taccggccgg tggccttcgt gtcgccgggt cccagcacgg cgccggggta cagcacgacc
     1321 agcggcagcc ccctcgagtg caggtcccag gctatctggt ctccttcgaa cttggtccgc
     1381 gcgtactcgc tccagcgtcg cctcccgaaa tcgctgctct ccgtgaccgg gtcgtcggcc
     1441 ggtgtcccgt acaccactat gctgctgacg tgggtgacct tgagcacgcc ggtctggagc
     1501 gcgcattcca tgacgttgcg agtgccggtg acgtttacgt cgcggtaaac ggcggggtcg
     1561 ggcagccaga agtcgtaggc ggccgccaag tggatgacgt ggtcacaccc ttccatgccg
     1621 cggagaaccg cctcgcgatc gacgacgtcg ccgcggacca cctccgcgcc gagcacctcg
     1681 atccgggtcg cgtcgctcgc gggtcgaacg aggcagcgca tcgagtggcc ggcgcgggcc
     1741 atgcgctccg ctacccgggt gccgatgaat ccagtcgctc cggtgatgaa cgctttcatt
     1801 tcgtgtacct cgagcatccg tgtcccatcc ccgcgcgaaa tcctggcagg gacgggcctg
     1861 accaatggtc agtctacgca ccacgccacg ccacgcctgt aggggcgggt cggcattcgg
     1921 agtgggtgca tcccggggcg aagagtgctt ctgccggccg cggagacgca gccggccgtg
     1981 gcagggcagc tcgcttgcgc ccgtccgatg tcacaaccgt cctccggtag cccccaggct
     2041 ctcacggaag ccacgccttc caccccgccg tcacacacca aacaggccga cggccgggtg
     2101 ggcgcggtcg aaggcgcgga gccgctcgag cagcgcgatc gacgccccgt tccctgcgtc
     2161 gacctgctcg aggagccggg atctttccct gatccagccg acacgggctc ccggaacgtc
     2221 ccgctccagg gcgcccgccc agacctccat ccagcggtcc cagttgcccg cggccgcggc
     2281 atcgcgcgcc gactggagcg tgggccagag cagtccgttc cggtcggcct cccgccggac
     2341 cgactccgcg aggatcggat tccgggaccc gagccgtcga aacacgaacc cgtccagctc
     2401 cggatccggg ccgtcgctga gcagcatgcg gacgaggtag tcatcgcggc gcacctcgtt
     2461 cggataaggc cggccgccga actgcgaatc caggcccgat cccaggatga acagcgcccg
     2521 gcggtcgttc tcctccgcgg cacgcacgag caggttgaac gcggcgtcac tccagaacgt
     2581 cgcgctcacc tcgctccgct ggaaggtcct ccggacgcgc agcgagacgg ggacatactc
     2641 ccctccgaca cccgcccacg cgaagtgcgc gtcggcgtcg taccagtccg cgagcgtcat
     2701 cgccgcgga
//
LOCUS       DBNHQZ010000198         4089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3719307, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000198 DBNHQZ010000000
VERSION     DBNHQZ010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3719307"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16YU_05295"
     CDS             complement(<1..344)
                     /locus_tag="AC16YU_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6064671.1"
                     /translation="MTLSFWGVRGSIPSPGPHTVRYGGNTTCASLELDADHTLVLDAG
                     TGIRMLGAAAPSGAHTYFVLLTHLHWDHIQGLPLFGPRNDPDTRIVFLGGLEPGWADM
                     ALQQFDGVRFPIR"
     gene            complement(443..2212)
                     /locus_tag="AC16YU_05300"
     CDS             complement(443..2212)
                     /locus_tag="AC16YU_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064672.1"
                     /translation="MLSAEGLRGRALVGLVVLLASPWVGGCLPGSDPEPVAFVREQCR
                     TCHAEEAGFSRFHRPLAIGCVSCHGGNPEARGADSAHTGMRAIPGNLADAAESCGAAG
                     CHAEIVGRVQGSLMATARGIVSVDRWVFGERPDPDGSDAITDLGDSPADSHLRQLCAS
                     CHMGAPKTAMGPLHPLSRGGGCLSCHLTYDPGSPHARISTYLPSDRCFGCHSRSGRIS
                     MNYQGWHETVLDYRGVDPSDTTLTILADGRVVERAPSDVHHDAGLECIDCHTSRETMG
                     DGRSHAHQEQAIETSCTSCHFSGPPPTLAWTELTDEDRRIVALREGREQPARRFLASD
                     RSGLALVNTYLDSLGRPVLEGKLDGLVRTLVGPAAACTAAGHKRLSCQSCHSAWAPQC
                     IGCHTQRTDSGEWVELVSDFRPDPPVLGVRESTGRIEPFIPGMIMTLNVEPLRGDPAE
                     AVGTGSFHRLYAPSVPHTTSAGGRTCESCHLDPLAVGFGRGTLRLDSSRRWLFTPTFA
                     PARDGLPADAWTPFLEPAARPNATRSDARPMTPDEQWAVLRVGACLTCHPAEIPRWEA
                     IYDDFDTALQSVTASCIDPARGG"
     gene            complement(2206..2781)
                     /locus_tag="AC16YU_05305"
     CDS             complement(2206..2781)
                     /locus_tag="AC16YU_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064673.1"
                     /translation="MKPLLVALAVLAALILLLAPPPLEETRLADPPGVKDRIPARTLA
                     TWVVSGDRHYHLLDIRPRERFLANAVRTAESMPADSLDEDAIRAIPSGHVLVLYGDSD
                     EDVVEPFRRVKAIHEQTYVLAGGHAACEREVLTLPDPPVDDTEEAWDEYRSRVALVRY
                     LTGESDVAPEPVQRSVRPVVRPRIKVSNEGC"
     gene            complement(2783..3388)
                     /locus_tag="AC16YU_05310"
     CDS             complement(2783..3388)
                     /locus_tag="AC16YU_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR6064674.1"
                     /translation="MFPLVDHLGFGTPTDYVLAFALGIGFGFFLERAGFGNANKLAMQ
                     FYFRDMAVLKVMFTAIVTAMTGLVVLSSSGVLDASLVYINPTYLWPGIVGGLIMGAGF
                     IIGGYCPGTALVGVVTGKVDALLSVLGGMLGMALFAESFGIPAVARFYDLDTAGITGR
                     ITLPDWLGVRPGLVALVVLVVAVAGFVAAEWAERRFNPVEA"
     gene            complement(3419..3937)
                     /locus_tag="AC16YU_05315"
     CDS             complement(3419..3937)
                     /locus_tag="AC16YU_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR6064675.1"
                     /translation="MEPKAAPYMNPYLAGIGLGLVLLAAFLVSGRGLGASGAIMRTVV
                     QAEKAVAQEHVDANAYLSSYGGGDRNPWDEWLVLETLGVLVGGLLSGAGGRRIRREIN
                     HGPRITPGRRLVFATIGGALFGYGARLARGCTSGVALTGGATLAVGSWITMLSIFAGA
                     YALAWFVRRLWI"
BASE COUNT          677 a         1364 c         1502 g          546 t
ORIGIN      
        1 cggatgggga accgcactcc gtcgaactgc tggagtgcca tgtcggccca gccgggctcg
       61 aggccgccca gaaagacgat ccgagtgtcg gggtcattcc tgggcccgaa gagcggcagc
      121 ccctggatat ggtcccagtg caggtgggtg agcagcacga agtacgtgtg ggcacccgac
      181 ggggcggccg caccgagcat ccggattccc gtcccggcat cgagcaccaa cgtatggtcg
      241 gcgtcgagtt cgagcgaagc acaggtcgtg ttgccgccgt accggacggt atgcggtccc
      301 ggagagggaa tggatcctcg aacgccccag aagctcagcg tcatgccgtc gcgatgcagc
      361 ggaacagagt gccgacgcaa gctacgatac gaggcatgcg tccggggtga tgtattacgc
      421 acgcgccatg atctgtcggg gattacccgc cacgggcagg gtcgatgcac gacgccgtca
      481 cggactgcag cgccgtgtcg aagtcgtcgt agatcgcttc ccatcgcggg atttcggccg
      541 gatggcatgt caggcaagcc ccgacccgga gcacggccca ctgctcgtca ggcgtcatgg
      601 gacgcgcatc actccgcgtc gcgttcggcc gggcggcggg ctccaggaac ggtgtccagg
      661 cgtccgccgg caggccgtcg cgggccgggg cgaaggtcgg cgtgaagagc cagcgccgcg
      721 aggagtccag ccggagcgtt ccgcggccga acccgacggc gagcggatcc aggtgacagg
      781 attcgcacgt acgtcctccg gcggacgtcg tatgcggcac ggacggcgcg tacagccggt
      841 ggaagctccc cgtgccgacg gcctccgccg ggtccccacg caggggctcc acgttaaggg
      901 tcatgatcat gccggggatg aagggctcga tccgccccgt gctctcccgg acgccgagga
      961 cgggcgggtc gggacggaag tccgagacga gctccaccca ctcgcccgaa tccgtgcgct
     1021 gcgtgtggca gccgatgcac tgcggcgccc acgccgagtg gcagctctgg caggagaggc
     1081 gcttatgacc ggccgccgtg cacgccgcgg cgggtccgac gagcgtcctc acaaggccgt
     1141 cgagcttgcc ctcgaggacg gggcggccca gcgagtccag gtaggtgttg accagggcga
     1201 gacccgaacg gtccgaggcg aggaatcggc gagcgggctg ctcccgtccc tcgcggagtg
     1261 cgacgatcct gcggtcctcg tcggtcagct ccgtccaggc cagggtcggc gggggtccgc
     1321 tgaagtggca ggacgtgcag gacgtctcga tagcctgttc ctggtgggcg tggctccttc
     1381 cgtcgcccat cgtctcgcgg gacgtgtggc agtcgatgca ctccaggccc gcatcatgat
     1441 gcacatcgga gggagcgcgt tccaccacgc ggccgtccgc cagaatggtc agcgtcgtgt
     1501 cggagggatc gactccccgg tagtccagga cggtttcgtg ccagccctgg tagttcatgc
     1561 tgatccggcc cgatcggcta tggcagccga agcagcggtc cgacggcagg taggtcgaga
     1621 tccgggcgtg gggcgatccg ggatcgtagg tcaggtggca cgaaagacac ccgccgccgc
     1681 gcgagagggg atgcagcggg cccatcgcgg tcttcggggc gcccatgtgg caggatgcgc
     1741 acagctggcg gaggtgcgag tcggcgggcg agtccccgag gtcggtgatg gcgtcggagc
     1801 cgtccggatc gggccgctcg ccgaacaccc agcggtccac cgacacgatg ccccgggcgg
     1861 tggccatgag ggatccctgc acgcggccca cgatctcggc gtggcatccc gcggcgccgc
     1921 acgactcggc cgcatcggcc aggttgcccg gaatggcccg catgcccgtg tgcgcggagt
     1981 ccgcgccgcg ggcctccggg ttgcccccgt ggcacgacac gcagccgatg gccaggggac
     2041 ggtggaagcg ggagaatccc gcctcttcgg cgtggcacgt gcggcactgc tcgcggacga
     2101 aggcgacggg ttcggggtcg ctgccgggca ggcagccgcc gacccacggg ctggcgagca
     2161 ggacgacgag gcccacgagc gcgcgaccgc ggaggccctc cgcgctcagc acccctcgtt
     2221 cgacaccttg atccgcgggc gcaccaccgg gcgcaccgac cgctggaccg gctccggagc
     2281 cacgtcggac tccccggtca ggtatcggac cagcgcgacc cggctccggt attcgtccca
     2341 tgcttcctcg gtgtcatcga cgggaggatc gggcagggta agcacctccc gttcgcaggc
     2401 cgcgtgccct cccgccagaa catacgtctg ttcgtggatc gctttcacgc ggcggaaggg
     2461 ttcgaccacg tcctcgtccg aatcgccata gagcaccagg acgtgtccgg acgggatggc
     2521 gcggatcgcg tcctcgtcga gcgagtcggc cggcatggac tccgccgtgc gcacggcgtt
     2581 cgccaggaac cgctcgcggg gccggatgtc caggaggtgg tagtgccggt cgcccgagac
     2641 gacccacgtg gccagggtgc gggcggggat ccggtccttc acgcccggcg gatcggcgag
     2701 gcgcgtctcc tcgagcggag gcggcgcgag gagcaggatg agggccgcga gaacggcgag
     2761 cgcgacgagc agcggcttca cgtcaggcct ccacggggtt gaagcgtctc tcggcccatt
     2821 cggcagcgac gaagccagcg accgcgacga ccaggacgac gagggcgacg agccccggcc
     2881 ggacgcccag ccagtcgggc agcgtgatcc gccccgtgat gcccgccgtg tccaggtcgt
     2941 agaaccgcgc cacggccgga atcccgaagc tctcggcgaa cagggccata ccgagcatgc
     3001 cgccgaggac ggagagcagg gcgtccacct tgcccgtcac cacgccgacg agcgccgtgc
     3061 ccgggcagta gcccccgatg atgaagccgg cccccatgat cagccccccg acgatgcccg
     3121 gccaaagata cgtgggattg atgtagacga gcgacgcgtc gagaacgccc gacgaggaga
     3181 gcacgacgag cccggtcatc gcggtcacga tggccgtgaa catgaccttg agcaccgcca
     3241 tgtcccggaa gtagaactgc atcgcgagct tgttcgcatt cccgaacccc gcgcgctcca
     3301 ggaagaaccc gaagccgatg ccgagggcga aggcgaggac gtagtcggtc ggagtgccga
     3361 atccgaggtg atcgacgagc gggaacatgg agaggaggcc ggagggatga tcgggagttc
     3421 agatccagag tcggcgcacg aaccaggcga gcgcgtaggc cccggcgaag atggacagca
     3481 tcgtgatcca ggagccgacg gcgagcgtcg ctccgcccgt cagggccacg ccgctcgtgc
     3541 atccgcgggc gagccgcgcg ccgtatccga acagggcgcc cccgatcgtg gcgaacacga
     3601 gccgccggcc cggcgtgatg cggggaccgt ggttgatctc ccggcggatg cggcgccctc
     3661 ccgcaccgga gagcaggccc ccgacgagga cgcccagggt ctccagcacg agccactcgt
     3721 cccacggatt gcggtcgccg ccgccgtagc tcgagaggta ggcgttggcg tccacgtgct
     3781 cctgggcgac ggccttttcg gcctggacca cggtgcgcat gatggcgccc gaggccccga
     3841 gtccgcggcc cgacacgagg aacgcggcga gcagcaccag gccgagccct atgccggcca
     3901 ggtagggatt catgtagggg gcggctttcg gttccatggc gggagtccgg ggtgcggatc
     3961 cggtggggtg tttcagtacg gcatccaggt cgtcacctgc ccggcttcga cgatgaagaa
     4021 gcggaagatg agcgagccgg caatcaccag gacgggcgcc aggaacgcgg ggagccggta
     4081 cccgcgaag
//
LOCUS       DBNHQZ010000199         3542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1687258, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000199 DBNHQZ010000000
VERSION     DBNHQZ010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1687258"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC16YU_05320"
     CDS             <1..914
                     /locus_tag="AC16YU_05320"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6064676.1"
                     /translation="LMIEPSARVAGMGNAGVSTVEGVTSAYFNPAALGRLEQSDAQFT
                     YGRWLADITYNYAAVALRLGSANTLHLSVTSLNSGEMDVRTVEQPLGTGERFTVQDMA
                     FGLGFGRRLTDRFAAGFQVNYIRETIWHSSLSAVGMNFGVFYELPFRAVLAASISNFG
                     TRGSYDGRDLRIRYDQDPDRYGDNSNLPAALETEDFALPIFFRVGVGMPFEMGSKNRF
                     SLHVDAYQPSDNTSSVSVGGEWMLMDLFALRAGYQNLFQDESETGLTLGTGLRYDVSR
                     YVFRVDYAWNDFGRIGDVQRFTVGFGF"
     gene            965..3520
                     /locus_tag="AC16YU_05325"
     CDS             965..3520
                     /locus_tag="AC16YU_05325"
                     /inference="COORDINATES: protein motif:HMM:NF021596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR6064677.1"
                     /translation="MIRRSLPVLLAGMLCTAGIRAQTVEVVLFDEDDPVGADYRDASL
                     GLAEDGDWLLLRGPNANRMPVATDQAYSGLSSGLLEWGHVFGGRWEMNIEPGGGERFD
                     LTGFDSLVVWLNGPDPVPGGELPRVGLRDIGGMPTPPAWLSTPENVHVDGAESGFSPG
                     SSTNVAVSARYLNVLSPEQRRLGYPENLVVTFSDAPLDTSTSAIGAPATPAHFRIVTE
                     DTGLQLDFSFRDLDGDRTLGAAGEFVDVLMLESESGTRARATWRIEVSDAPDPVAPPD
                     EGDAYRLVVGFDEFTLDADPGTWQRVSLPIGDLLAVDGFTESQVWLPWFANGGLTTGV
                     RTLWVDRITAVRNDDEAFLDLLQERTFDFFWHETNPSNGLVKDRTTDSSPSSIAAVGF
                     GLSAYTVGIDRGWITREQGRLRTLNTLRFFWNAPQGTGPNVTGYRGFFYHFLDMTTGL
                     RRGTTELSTIDTALLLGGVLHVGEYFDGPDPEEEEIRALADSIYRRVDWKWAQNHPPA
                     ISHGWRPPGEANSGFISNDWIGYNEAMILYILALGSPTHPVDPDAWDAWTSGYGSDWR
                     TEFGYTYLVFPPLFGHQYSHVWLDFRGIRDAFMRLKGIDYFENSRRATLAQRAYHAAN
                     PKGWPNYGADEWGLTASDTPTGYRARGAPPAQNDDGTLAPTAPGGSIAFAPEEALAAL
                     WTMYRSQPRLWGPYGFRDAYNVSVNWVDEQYLGIDQGPILLMAENHRTGRVWDVFMRN
                     EDIRRGLVRAGFDNVPSPIEPELPSRLTALESYPNPFAGRASIRYRVAIPGPVRLAVY
                     DLLGREISVLSDGMEVPGSREVVFEASGLAPGVYLARLTTGEGSITAKLVVAR"
BASE COUNT          567 a         1195 c         1213 g          567 t
ORIGIN      
        1 tcctgatgat cgagcccagc gcgcgcgtgg ccggcatggg caacgccggg gtgtccacgg
       61 tcgagggcgt gacgtcggcc tacttcaacc ccgcggcgct cggacgcctc gagcagtcgg
      121 acgcccagtt cacgtacgga cggtggctcg cggacatcac gtacaactac gcggccgtcg
      181 ccctgcggct cggaagcgcg aacacgctgc acctgtccgt cacgtccctg aactccggcg
      241 agatggacgt ccggaccgtg gagcagccgc tcgggacggg cgagcggttc acggttcagg
      301 acatggcctt cgggctgggc ttcggccggc ggctcacgga ccgcttcgcg gccggcttcc
      361 aggtgaacta cattcgggag acgatctggc actcgagcct gagcgcggtc gggatgaact
      421 tcggcgtctt ctacgagctg cccttccgcg ccgtcctggc cgcgagcatc tcgaatttcg
      481 ggacccgcgg cagctacgac gggcgggacc tgcgtatccg gtacgaccag gatcccgacc
      541 gctacggcga caacagcaat ctccccgctg cgctcgagac ggaggacttc gcgctcccga
      601 tcttcttccg cgtgggcgtg ggcatgccgt tcgagatggg ttcgaagaac cggttcagcc
      661 tgcacgtgga cgcctaccag ccgagcgaca acacgagcag cgtgagcgtc ggaggggagt
      721 ggatgctgat ggacctcttc gcgctccgcg cgggatacca gaacctgttc caggacgaat
      781 ccgaaaccgg gctcacgctc ggaacgggtc tgaggtacga cgtctcccgc tacgtattcc
      841 gcgtagacta cgcctggaac gacttcggtc ggatcgggga cgtgcagcgg ttcacggtcg
      901 gattcggatt ctgagaccac tccgaccgcc cgatctggac ctctcggacc gtacggtgcc
      961 cgtcatgatc cgtcgctcgc taccggtgct gctcgcgggc atgctgtgca cggcggggat
     1021 ccgggcgcag accgtcgagg tcgtcctgtt cgacgaggac gatccggtcg gagcggacta
     1081 ccgcgacgca tcgctcggcc tcgcggagga cggcgactgg ctgctgctcc gcggtccgaa
     1141 cgcgaaccgg atgcccgtag cgacggatca ggcatacagc gggctctcga gtggtctcct
     1201 ggagtggggg cacgtgttcg gaggccgctg ggagatgaac atcgagccgg gagggggaga
     1261 gcgcttcgat ctgaccggct tcgacagcct ggtcgtctgg ctgaacggcc cggaccccgt
     1321 gccgggcggc gagctcccgc gcgtcggcct gcgtgacatc gggggcatgc ccacgccgcc
     1381 cgcatggctc agcactccgg agaacgtcca cgtggacggc gccgaatcgg gcttctcccc
     1441 ggggagctcg acgaacgtcg ccgtgtccgc gcgctatctg aacgtcctgt ccccggaaca
     1501 gcgaaggctc ggataccctg aaaacctggt ggtgacgttc tcggacgccc ccctcgatac
     1561 ctcgacctcg gccataggag cgccggcgac ccctgcgcat ttccggatcg tgacggaaga
     1621 cacgggcctg cagctcgact tttcattccg tgacctcgac ggggaccgca cgctgggcgc
     1681 tgcgggcgag ttcgtcgacg tgctcatgct ggagtccgaa tccgggaccc gcgcccgcgc
     1741 gacctggagg atcgaggtga gcgacgcccc ggatccggtc gcgccgccgg acgagggcga
     1801 cgcgtatcgg ctcgtcgtcg gattcgacga attcacgctc gacgccgacc ccgggacgtg
     1861 gcagcgggtc agccttccga tcggcgacct gctcgccgtg gacggtttca cggaatcgca
     1921 ggtctggctc ccgtggttcg ccaacggcgg gctgacgacg ggcgtgcgca ccctgtgggt
     1981 cgaccggatc acggcggtgc gaaacgacga cgaggccttc ctggacctgc tccaggagcg
     2041 gaccttcgat ttcttctggc acgaaaccaa tccctcgaac gggctggtca aggaccggac
     2101 gacggactcg tccccgtcga gcatcgcggc ggtcgggttc ggcctgtccg cctacacggt
     2161 ggggatcgac cgcggatgga tcacgaggga gcagggccgc ctgcgcacgc tgaacacgct
     2221 ccggttcttc tggaatgcgc cccagggtac cgggccgaac gtgaccgggt accgcggatt
     2281 cttctaccac tttctggaca tgacgaccgg cctgcgccgg gggacgaccg agctctcgac
     2341 catcgacacc gccctgctcc tgggcggcgt gcttcacgtc ggcgagtatt tcgacgggcc
     2401 ggatcccgaa gaggaggaga tccgggcgct ggccgactcg atctaccgta gggtggactg
     2461 gaagtgggcg cagaaccacc cgccggccat cagtcacggg tggcgtccgc ccggggaagc
     2521 caattccggg ttcatctcga atgactggat cgggtacaac gaggcgatga tcctgtacat
     2581 cctcgccctc ggctcgccga cccatcccgt ggatcccgac gcgtgggacg cctggacgag
     2641 cggatacgga agcgactggc gcaccgagtt cgggtacacg tacctcgtct ttccgcccct
     2701 cttcggccac cagtacagcc acgtctggct cgacttccgg ggcatccgcg atgcgttcat
     2761 gcggttgaag ggcatcgact atttcgagaa ctcacgccgg gcgacgctgg cgcagcgcgc
     2821 gtaccacgcc gccaatccga agggatggcc gaactacggg gccgacgagt gggggctgac
     2881 cgcgtccgac acgccgaccg gctaccgggc gcgcggcgcg ccgcccgccc agaacgacga
     2941 cgggaccctc gcgccgaccg ctcccggggg gtccatcgcg ttcgcgccgg aagaggccct
     3001 cgcggcgctc tggaccatgt accggtcgca gccgcggctg tgggggccct acggctttcg
     3061 ggacgcgtac aacgtgtccg tgaactgggt cgacgagcag tacctcggga tcgaccaggg
     3121 cccgatcctc ctcatggccg aaaaccatcg gacgggacgc gtctgggacg tcttcatgcg
     3181 gaacgaggac attcggcgcg gcctggtccg ggccggattc gacaacgtgc cgagcccgat
     3241 cgagccggag cttccctccc ggctcaccgc gctcgagagc taccccaatc cgttcgccgg
     3301 acgggccagc atccggtacc gggtcgcaat tcccgggccg gtccggctcg ccgtgtacga
     3361 tctgctcggc cgggaaatct cggtcctgtc ggacgggatg gaggtgcccg ggagccggga
     3421 ggtcgttttc gaagcctccg gcctggcgcc cggcgtctat cttgcacgcc tgacgacggg
     3481 cgaaggctcg atcaccgcga agctggtcgt cgcccgctga accgaaacca cgagacgaag
     3541 tg
//
LOCUS       DBNHQZ010000200         4795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_738792, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000200 DBNHQZ010000000
VERSION     DBNHQZ010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_738792"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC16YU_05330"
     CDS             complement(<1..229)
                     /locus_tag="AC16YU_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064678.1"
                     /translation="MGNGWSRHTASPARPRRGKRTWRQYWVSGGYSIQIEGGLDDEGR
                     MVLEGELHAYRSRQAVPFRGTWTPNPDGSVRQ"
     gene            334..3381
                     /locus_tag="AC16YU_05335"
     CDS             334..3381
                     /locus_tag="AC16YU_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR6064679.1"
                     /translation="MNANRVQGTWVFLVAALSTMLMTTPVTAQQAGTIRGEVLDAISN
                     RPLANAQVSVAGTGRGALANSNGQFLVVNVPPGTHTVQVELIGYGAQEQEVDVQAGQV
                     AQVSFRMAQSALELDAVVVTGTPGQTQRRAIGNSVSTFQATQVTEAVPVANVQELRQA
                     RTPGLTLIADGGAAGDGSAIRLRGSGSIEGRFEPVIYVDGIRVAGGTNSFDDQCGSVT
                     HCTDALDFLNPNDIESVEVIKGPAASTLYGAEAAAGVIQIITKKGRSGQAGIQWSAGV
                     DVGTSDWHLQRPVTYWNCTPSQVGSASFPGCTEPGLRTFEPFEHPAALRGNADYPECQ
                     RPEPFDVRSFCAPDSNGAGQYSFNLSARGGGELFNFFASMEKSDEQGMFPNNYARRTG
                     GRANFGFTPSDELSFNVNVGYSRNHVRMPLANNSSNSILRNGMRGRADAAYQYEVGYR
                     NFGPTLANEWNMQTQSERYTIGTTISYNPFSWWQNRLVLGLDNNNREVRDFTPPDPRP
                     EAPHGTTRTTGLIDYTTPTSHFWTVDYSATLSADINEDYNSQFSAGMQLNATKFESFG
                     ITGEGLVSRNLNLVGSAATTRGDQNFQEQTSLGFYVQEQVGFRNRLFATAAVRIDDNS
                     AFGRDFSLVVYPKAQLSYVISDEDFFDVGWVDQLKLRGAWGQAGNPPDPFTADRTYSP
                     AVSVAADGQVVNILRPSSYGNPDLKAETGSELELGFEASMLDSRMSLDVSYYQQNTKD
                     ALIAIPDPPSSGFSDEHFANVGEIKNSGVEVFLTATPVYRRNLQWDATLAFSANSNEL
                     VDFGDAPVEELAFGSFATVQKHIEGYPLGGYWGITGTVEDDLSVTLSDEEYVGPSLPT
                     REIGLTNTFTLFEDFRLFANLDYKGGHYQWCAICSVRSRFDQNTYTINRPDADPEEVA
                     LVTSNQTRRWISEADFIKLREVSITYQLPSSALERIGVNNGSVSLAARNLWMWTKYGF
                     PDGEGLGSPDPEVNFSSTSTYSRTDYASIPMLRSFSASVRFAF"
     gene            3399..4760
                     /locus_tag="AC16YU_05340"
     CDS             3399..4760
                     /locus_tag="AC16YU_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064680.1"
                     /translation="MNKTYSTRAVRTTVGGLLLAVAACSSFDSLLEVQNPDELDEGFL
                     QDEALIEVLVNSVTGDFEDAFDDPFIWRGSMFTDESLTGINWEQTARLSQRIVEFDEG
                     DADMMFSEISAAVQQGDSISSRLTTLLASPNDDPRMATTLIYAGYSYILLADAMCEAT
                     VNVGAEIHEPLALYDFAVDRLESGLAVAQAAGEDDLVNLARVGLTRAHLNLGNYGQVE
                     AIADQVPDDFRFYARYSDADPDVYNVLESRVTGGNHSLGVHPNFTAGGPAMFDDPGDK
                     TALLTDPRVQHFDSWRLGHNQLTKLYTPYQSLMMSEFNGETLADGGEPVRIGRGTNIA
                     FASGLEAQHNKMEALAELDIVANQAEILAFVNDRRDFGNQGTIVATGDALVMELRDQR
                     GRDLFLAGYRLGDLRRWLRGGDDMFPSGQHPNAQWGNYGDATCFPMPLEEYEGNPNIP
                     VPS"
BASE COUNT          899 a         1556 c         1546 g          794 t
ORIGIN      
        1 gctgccggac cgagccgtcg ggattgggcg tccacgtgcc gcggaagggg acggcctgac
       61 gggagcggta ggcgtggagc tcgccctcga ggaccatccg gccctcgtcg tcgagtccac
      121 cctcgatctg aatggagtag ccccccgaga cccagtactg cctccacgtc cgcttgccac
      181 gtcgcgggcg tgcaggggag gccgtgtgtc gcgaccaacc gttccccacg atccggctct
      241 gtgcttcagt tgcgaatgtt ctgtcgtcga agccgaagcc cctccatatt ggcaccaccc
      301 ccccaaggtg aactcttgac aggtaggtgc gagatgaacg caaaccgagt ccaggggacc
      361 tgggtcttcc tcgtggcagc cttgagcaca atgctcatga cgacgcccgt gacggctcag
      421 caggcgggaa ccatccgggg cgaggtcctg gatgccatca gcaacaggcc cctggccaac
      481 gcccaggtgt cggtggccgg caccgggcgg ggcgctctgg ccaattccaa cggccagttt
      541 ctggtcgtga acgtccctcc gggcacacac acggtgcagg tggagctcat cggttacggt
      601 gcccaggagc aggaggtcga cgtgcaggcc ggccaggtcg cccaggtgag cttccgcatg
      661 gcgcagtccg ccctcgagct cgacgcggtg gtggtcaccg gaacgcccgg gcagacgcaa
      721 cgccgagcca tcggcaactc cgtctccacg ttccaggcga cacaggtgac ggaagcggtg
      781 cccgtggcga acgtccagga gctccgccag gcacggacac cgggcctcac cctgatcgcc
      841 gacggtgggg cggcaggtga cggctccgcc atccgcctca gggggtcggg ctccatcgaa
      901 ggacgcttcg agcccgtgat ctacgtggac ggaatccggg tcgcgggcgg cacgaactcg
      961 ttcgacgacc agtgtggctc cgtgacgcac tgcacggacg cgctcgactt cctcaacccg
     1021 aacgacatcg agtcggtcga ggtgatcaaa gggccggcgg cgtcgacgct ctacggggcg
     1081 gaagcggcgg ccggcgtgat ccagatcatc acgaagaagg gtcgctccgg gcaggcggga
     1141 atccagtgga gcgccggcgt cgacgtcggc acgtccgact ggcacctgca gcgtccggtc
     1201 acctactgga actgcacgcc gagtcaggtg ggttccgcgt ccttcccggg atgcaccgag
     1261 ccgggtctca gaaccttcga gccgttcgag caccctgcgg cgctgcgtgg gaacgcggac
     1321 tacccggagt gccagcggcc cgagccgttc gacgtccgga gcttctgcgc gcccgactcg
     1381 aacggggccg gtcagtactc gttcaacctt tcggcgcgtg gcggtggaga gctcttcaac
     1441 ttcttcgcct ccatggagaa gagcgacgag cagggcatgt tcccgaacaa ctacgcgcgc
     1501 cggacgggcg gacgggccaa cttcggcttc acgccgagcg acgagctgtc cttcaacgtg
     1561 aacgtcggct attcgcggaa ccacgtacgg atgccgctcg ccaacaactc gtcgaactcg
     1621 attcttcgca acggcatgcg tggacgggcg gacgccgcct atcagtacga ggtgggctac
     1681 cggaacttcg gtccgacgct cgccaacgaa tggaacatgc agacgcagtc cgagcggtac
     1741 acgatcggga ccaccatcag ctacaacccc ttcagctggt ggcagaaccg gctcgtgctc
     1801 ggcttggaca acaacaaccg cgaggttcgg gacttcacac caccggaccc gaggcctgaa
     1861 gctcctcatg gcacgacgcg cacgactggc ctcatcgact acacgacgcc gacgtcccat
     1921 ttctggacgg tcgactactc cgccacgctg agcgcggaca tcaacgagga ctacaactcg
     1981 cagttcagcg ccggcatgca gctcaacgcc accaaattcg agtcgttcgg tatcacgggc
     2041 gagggattgg ttagccggaa cctcaacctc gtcggctccg cggcgacgac gcgcggtgat
     2101 cagaacttcc aggagcagac ctccctgggc ttctacgtcc aggagcaggt cggcttccgc
     2161 aaccgcctct tcgccacggc ggcggtgcgc atcgacgaca actctgcatt cggacgcgac
     2221 ttcagcttgg tcgtctaccc gaaggctcag ctttcgtacg ttatcagtga cgaagatttc
     2281 ttcgacgtcg gctgggtcga ccagctcaag cttcgaggcg cgtggggtca ggccggcaac
     2341 ccgcccgatc cgttcacggc cgacaggact tactcgccgg ctgtctcagt agccgccgac
     2401 ggacaggtgg tgaatatcct ccgtccctcg tcatacggaa atccggacct caaagcggaa
     2461 acgggctcgg agctcgagct gggcttcgag gcatctatgc tcgatagtcg catgagcctt
     2521 gacgtcagct actaccaaca gaataccaag gacgcgctta tcgcgattcc ggacccgccc
     2581 tccagcgggt tcagtgacga gcacttcgcc aatgtcggcg agatcaagaa cagcggggtg
     2641 gaggtcttcc tgacggccac gccggtctac cgacggaacc tgcagtggga cgcgacgctc
     2701 gcgttcagcg cgaactcgaa cgagctggtc gacttcggtg atgcgccggt cgaagagctt
     2761 gcgttcggct cgttcgcgac cgtccagaag cacatcgagg ggtatcccct cggcggctac
     2821 tggggaatca ccgggaccgt cgaagacgac ctcagcgtca cgctgagcga cgaggagtat
     2881 gtcgggccct cactcccgac ccgtgaaatt gggctcacca acaccttcac gctcttcgag
     2941 gatttccgtc ttttcgcgaa cctcgactac aaggggggtc actaccagtg gtgcgccatc
     3001 tgctcggtgc ggagtcgctt cgaccagaac acctacacca tcaaccgccc cgacgcggac
     3061 cctgaggagg tcgcgctggt gaccagtaac cagacgcggc gttggatctc cgaggcggac
     3121 ttcatcaagc tccgagaggt ctcgatcacg taccagttgc cgagctcggc gctcgagcgg
     3181 atcggggtca acaatggcag cgtgtcgttg gcggctcgaa atctttggat gtggacgaag
     3241 tatggattcc cggacgggga aggactcggc tcgcccgacc cggaagtgaa cttcagctcc
     3301 acgtcgacct acagtcgcac ggactacgcc tcgattccga tgctgcggag cttctccgcc
     3361 agcgttcggt tcgcattctg accgaaggag gtgacgacat gaacaagaca tactcgaccc
     3421 gagcggtccg aacgaccgtg ggcgggctcc tcctggcggt cgcggcttgc tcgagtttcg
     3481 actcgctcct ggaggtacag aacccggacg aactcgacga aggcttcctc caggacgagg
     3541 ccctcatcga ggtcctggtg aacagtgtta ccggcgattt cgaggatgcc ttcgacgacc
     3601 ccttcatctg gagggggagc atgttcacgg acgagtcgct caccggaatc aactgggagc
     3661 aaacggcccg cctgagccag cgtatcgtcg agttcgacga aggcgacgcc gacatgatgt
     3721 tctccgagat cagcgccgcg gtgcagcagg gtgactcgat ctccagcagg ctcacgacgt
     3781 tgctagcgag cccgaacgac gacccgcgca tggcgaccac gctgatctac gcggggtatt
     3841 cgtacatcct gctggccgac gccatgtgcg aggccacggt caatgtcggc gccgagatcc
     3901 acgagccact ggcgctgtat gatttcgcgg tggatcgcct ggagagcggg ctcgccgtgg
     3961 cacaggccgc cggtgaggac gacctggtca acctcgcgcg tgtcggcctg acgcgcgcgc
     4021 atcttaacct cggcaactac ggccaggtcg aggccatcgc ggaccaggtg ccggacgatt
     4081 tccgcttcta cgctcggtac agcgacgcgg acccggacgt gtacaacgtg ctggagtcac
     4141 gggtgacggg cggaaaccat tcgctcggcg tccaccccaa cttcacggcc ggtggccccg
     4201 cgatgttcga cgatccgggt gacaagacgg cattgctgac ggacccgcgg gttcagcact
     4261 tcgactcgtg gcggctgggg cacaaccagc tcacgaagct gtacacgccc taccagagtc
     4321 tcatgatgtc cgagttcaac ggagagacgc tggcggacgg cggagagccc gtgaggatcg
     4381 ggcgcggcac gaacatcgcg ttcgcgtccg ggctcgaggc gcagcacaac aagatggagg
     4441 ctctcgccga gctcgatatc gtggcgaacc aggcagagat cctcgccttc gtcaacgatc
     4501 gacgtgactt cgggaaccaa ggtacgatcg tggctacggg cgacgccctg gtcatggagc
     4561 tgagagacca gcggggtcgg gacctgttcc tggccggata ccggctcggc gatctgcgtc
     4621 gctggctcag gggtggggac gacatgttcc ccagcgggca gcacccgaac gcccagtggg
     4681 gcaactacgg ggacgcgacc tgcttcccga tgcccctcga ggagtacgag ggcaacccga
     4741 acattccggt gccgtcctga tgtaggcacg gagcgtgcgg cggctacgcg agccg
//
LOCUS       DBNHQZ010000201         7048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_332680, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000201 DBNHQZ010000000
VERSION     DBNHQZ010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_332680"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="AC16YU_05345"
     CDS             <1..857
                     /locus_tag="AC16YU_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR6064681.1"
                     /translation="GDAPAPGPRKQYYQRFDGYSRFLHLLVILSFLSLAVTGMTIKFS
                     GVGIFQTLSRLLGGYQVTGFVHRTAAIVTFVYFFLHLYYVIRRMRRARMRPIEILKGP
                     NTLMFQKQDLVDFGKTIKWFLGIGPRPDYGRWTYWEKFDYFAVFWGVAVIGFSGLILW
                     FPEWFTGLGLPGWLVNVATIIHSDEALLATGFIFTVHFFNGHFRPDKFPMDPVIFTGR
                     VEVEELKIDRPRLYRKLVESGELERGLVDAPNPLLEKWARIFGLTALTIGLATVVMII
                     YSMIFLYR"
     gene            935..3388
                     /locus_tag="AC16YU_05350"
     CDS             935..3388
                     /locus_tag="AC16YU_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6064682.1"
                     /translation="MLLATLALPHAARAGDPPVRIRPLAAHETVRPAQAPAHRFAADP
                     DDCFMCHDDESLTMERGGREVSLYVDPSAWSGAPHAGLDCVDCHVGFDPDEMPHADPI
                     APVACTTCHGAEVRSARMSNHPDAANCASCHDDVHTAPGTLADNSTCAACHAAEQAVV
                     EAGSHGHNGAAPVCLDCHGAHRFAPADTDGCLSCHGEREFVDEHVTGENLAAILSYSE
                     SIHGQVIECSDCHSGHDLRPASDPESRVHHSNIASTCATCHDQEAEHYRDSEHGRALA
                     AGFEGAPTCTDCHGEHDIRTITDSESPMSRQHEVEVCLSCHLDSENVQARMTHTAGFV
                     AGYELSVHGRAAAAGSDRAPVCSDCHGAHSEMKASAPGSKVNKFNLVQTCGDCHVDIG
                     DQFAASIHGAALEDGNQDAPTCTDCHSEHSITSTDDPAAPVALLNVSEAVCRPCHESY
                     QLSAKYGFPSDRAETFGDSFHGFAGRLGQDEVANCASCHGVHDILPSSDPNSRVHREN
                     LAATCGTCHPGADERFSEGSVHIVRTAEGDRLLFWIGRVYLIASLSIIGLMLLHNGMD
                     WVRKTRDVIHARMRGTGLPHTPVHPGPRKLYERMTVADRWQHALLALSFIMLVITGFM
                     LRYPDAWWVTLSKEVFGGHLVQVRSLTHRISGVVMLAVSAWHVGFVVFTPRGRQFLKD
                     MWWKRTDLTEFMQSLRYMAGLSDERPRYDRFNYVEKAEYWALVWGTVVMGATGLILWF
                     EGGFAKMTFDVSETVHLYEAWLAFLAIVVWHFYYVIFNPDVYPLNVTFLTGKVTEEDM
                     EHEHPRELERLKAGEEGNA"
     gene            3533..3976
                     /locus_tag="AC16YU_05355"
     CDS             3533..3976
                     /locus_tag="AC16YU_05355"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR6064683.1"
                     /translation="MVEPKRSRRTFLNFVLGGGLVGWLGAVLYPVASYLRPPEDKGAD
                     VASVKWGPEADFRPGTSAIVKFGRKPVILIRTESGEFRAFDATCTHLDCTVQYRADQR
                     DIWCACHNGVYDLRGQNVSGPPPRPLGAYAVNIVDGDVVIARMEA"
     gene            3981..5063
                     /locus_tag="AC16YU_05360"
     CDS             3981..5063
                     /locus_tag="AC16YU_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6064684.1"
                     /translation="MSKGPKRGVFGWLDDRYSLGPLVNYMRHKEVPVHRHTIWYYMGG
                     VTLFLFIVQLFTGILLLLYYRPGAESAYESVRFLMNKVPFGWLFRAVHSWGANLFILF
                     AFVHMFSTYFTRAYAKPRELTWVTGFILLALGMGFGFSGYLLPWNELAFFATKVGTDI
                     AGVLPIVGDGIKIFLRGGDDVTGATLIRFYAFHIALIPAIFTAFLGIHLLFVQRQGMH
                     EPESVRLLPDGKRRMMAFFPNFVLRDLFVWLIVLNVLLFLAVFYPWELGLEADPFAPA
                     PPGIKPEWYFMFMFQTLKLVPAHVLGIEGEVLGVLVFMVAGLAWMLVPFWEIRSRSTG
                     KLKPMAVIGFLALAFIVVMTIVGYLV"
     gene            5072..6331
                     /locus_tag="AC16YU_05365"
     CDS             5072..6331
                     /locus_tag="AC16YU_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6064685.1"
                     /translation="MRRRPGGGLSVWLAVLAAGFAALPAAAQEIPDALSGDQCVACHS
                     DVGYLPEGMILAGPHMRVELSCAGCHGGDNTTDDEDIAHAGTFRGVPDETAIPAFCGR
                     CHSDLGFMRAYSPSASTDQESNYFTSVHGQRLREGDTKVAQCASCHTAHAVLPASDAR
                     STVHPMNVPSLCNECHGDAEYMAEYGIRTTQYRDYAQSVHGTALLEREDLGAPACNDC
                     HGNHGAQPPGIESIEHVCGMCHVNNAEFFSATSMARAFAEDELHACEECHGIHDVQKT
                     FDAMVGTGDDSVCTVCHEEGDDGYAAAEAIYAQLVALVAAQDSASTLAERVREIGMDD
                     IEIGFLLRDAHESLIQSRTLVHTFDPERTAEATTVGMGKATEAIELGHAEIRNFRFRR
                     FGFGIATLLITVLTVALFFKIRDMERS"
     gene            6468..>7048
                     /locus_tag="AC16YU_05370"
     CDS             6468..>7048
                     /locus_tag="AC16YU_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR6064686.1"
                     /translation="MKWTERARAKWIEGRDTFRSWPRRKRLVVGWVGGAVVLVLSIVA
                     SAEYTSQPSFCPTCHYMEPYYESWQTSGAHADIACATCHFPPGIAGTIKGKAEGLVQV
                     VNYLASSYTRRKPWAEIDDVSCMQSGCHDTRLEGEVTFKGVAFDHAPHLEGLRRGKQL
                     RCTSCHSQIVQGDHITVTETTCFLCHMKVGGEVS"
BASE COUNT         1138 a         2331 c         2375 g         1204 t
ORIGIN      
        1 cgggcgatgc ccctgcgccc ggaccgcgga agcagtacta ccagcggttc gacgggtact
       61 cgcgcttcct gcatctcctc gtcatcctga gcttcctctc gctcgcggtc accgggatga
      121 ccatcaagtt ctcgggcgtg ggcatcttcc agaccctttc ccggctcctt ggcggctacc
      181 aggtgacggg attcgtgcac cggaccgcgg cgatcgtcac gttcgtctac ttcttcctgc
      241 atctctacta cgtgatccgg cggatgcggc gcgcacggat gcgcccgatc gagatcctga
      301 agggcccgaa cacgctgatg ttccagaagc aggacctggt cgacttcggg aagacaatca
      361 agtggttcct gggcatcggc ccccgcccgg actacggccg ctggacgtac tgggagaagt
      421 tcgactactt cgcggtcttc tggggggtcg ccgtgatcgg gttcagcggc ctgatcctgt
      481 ggttcccgga gtggttcacc ggactgggat tgccgggatg gctggtcaac gtggccacca
      541 tcatacacag cgacgaggcg ctgctcgcca cgggcttcat cttcacggtc catttcttca
      601 acggccattt ccgccccgac aagttcccga tggatcccgt gatcttcacc gggcgggtcg
      661 aggtggagga gctgaagatc gaccgtccga gactctaccg gaagctggtc gagtcgggag
      721 agctggagcg cgggctggtg gacgccccga atccgttgct tgagaaatgg gcgcgcatat
      781 tcgggctgac tgccctgacg ataggtctgg ccaccgtcgt gatgatcatc tactcgatga
      841 tcttcctgta ccgctaacgg cggcagccga gacgtccggc ccgtgcggcc ggacgatccg
      901 gaggccggtt ccggcgaccg gcctcctggc cgctgtgctg ctggcgaccc tggcgctccc
      961 gcacgcggcg cgcgcgggcg acccgccggt ccgaatccgc cccctcgcgg cccacgagac
     1021 ggtccggccg gcgcaggcgc cggcgcaccg cttcgccgcg gacccggacg actgcttcat
     1081 gtgccacgac gacgagagcc tgaccatgga gcggggcggc cgcgaggtgt ccctctacgt
     1141 cgatccgtcc gcctggtccg gtgcgccgca cgcgggcctg gactgcgtgg actgtcacgt
     1201 cggattcgat cccgacgaga tgccccacgc cgacccgatc gcccccgtgg cctgcaccac
     1261 ctgccacggc gcggaagtgc gctcggcccg gatgtcgaac catcccgacg cggccaactg
     1321 cgcgtcctgc cacgacgacg tgcacacggc tccggggacg ctcgcggaca attcgacgtg
     1381 cgcagcctgc cacgccgctg agcaggccgt cgtggaggcg ggttcgcacg gccacaacgg
     1441 cgccgcgccg gtctgcctgg attgccacgg cgcgcaccgg ttcgccccgg ccgatacgga
     1501 cggctgcctc tcgtgccacg gcgagcgtga gttcgtcgac gagcacgtga cgggagagaa
     1561 cctggccgca atcctgtcct acagcgaaag catccacggc caggtcatcg agtgctccga
     1621 ctgtcacagc gggcacgacc tgcggccggc gtccgacccg gagtcccggg tccaccactc
     1681 gaacatcgcc tcgacctgcg ccacgtgcca cgaccaggag gcggagcatt accgggattc
     1741 ggagcacgga cgggccctcg cggccggatt cgagggggcg ccgacctgca cggactgcca
     1801 cggcgagcac gacatccgga ccatcacgga ctcggagtcc ccgatgagcc ggcagcacga
     1861 ggtggaggtg tgcctgagct gtcacctgga ctcggagaac gtccaggcgc gcatgaccca
     1921 cacggccgga ttcgtcgcgg ggtacgagct gagcgtgcac ggtcgggcgg ccgccgccgg
     1981 gagtgaccgg gcgccggtgt gctccgactg ccacggagcg cacagcgaga tgaaggccag
     2041 tgccccgggc tccaaggtca acaagttcaa cctggtccag acctgcggcg actgtcacgt
     2101 ggacatcggt gaccagttcg ccgcctcgat ccacggggcg gccctggagg acggcaacca
     2161 ggacgcgccg acctgcaccg actgccacag cgagcattcg atcaccagca cggacgaccc
     2221 tgcggcgccg gtggccttgc tgaacgtatc ggaggccgtc tgccggccgt gccacgaatc
     2281 ctaccagctt tcggcgaagt acggattccc gtccgaccgc gccgagacgt tcggcgacag
     2341 cttccacggg ttcgccgggc ggctgggcca ggatgaggtc gcgaactgcg ccagctgtca
     2401 cggcgtgcac gacatcctcc cgtcgagcga cccgaactcc cgcgtgcacc gggagaacct
     2461 tgcggccacc tgcggcacgt gccatcccgg agcggacgag cggttcagcg agggaagcgt
     2521 ccacatcgtc cggacggcgg agggggaccg cctgctcttc tggatcggcc gggtgtacct
     2581 catcgcgagc ctctcgatca tcggactcat gctgctccac aacgggatgg actgggtgcg
     2641 caagacccgg gacgtgatcc atgcccgcat gcgcggcacc gggctcccgc atacgccggt
     2701 tcacccgggg cctcggaagc tgtacgagcg gatgaccgtc gcggatcggt ggcagcatgc
     2761 gctcctggcc ctgagcttca tcatgctggt catcacgggc ttcatgctgc gctacccgga
     2821 cgcctggtgg gtgaccctgt ccaaggaagt gttcggaggg cacctggtcc aggtgcggag
     2881 cctgacgcac cggatttccg gggtggtcat gctcgcggta tccgcgtggc acgtggggtt
     2941 cgtcgtcttc acgccccgag gccgccagtt cctgaaggac atgtggtgga agcggacgga
     3001 cctgaccgag ttcatgcagt cccttcggta catggcgggc ctttcggacg aacgcccgcg
     3061 ctacgatcgg ttcaattacg tagagaaggc ggagtactgg gcgctcgtct gggggacggt
     3121 ggtcatgggg gcgaccgggc tcatcctgtg gttcgagggc ggcttcgcga agatgacctt
     3181 tgatgtctcg gagactgtgc acctgtacga ggcctggctt gcgttcctgg ccatcgtagt
     3241 gtggcatttc tactacgtca tcttcaaccc cgacgtgtac ccgctcaacg tgactttcct
     3301 gacgggaaag gtgaccgagg aggacatgga gcacgagcat ccgcgcgagc tcgagcgact
     3361 gaaggccggg gaggagggca acgcctgacg gggcgaaccg gcacggaaga gcccgaagag
     3421 catgtcgcag gcacgggcat tccctacatc catgtagggc gtcgggacct tgacagggtc
     3481 cggggatctc cgtattgagg gtcaagcaat cccttcaagc ctctacaagc ccatggtcga
     3541 acccaaacgg agccggcgca ccttcctgaa cttcgtgctg ggagggggcc tcgtcggctg
     3601 gctcggcgcc gtgctgtacc cggtggcctc gtaccttcgt ccaccggagg acaagggcgc
     3661 ggacgtggct tccgtgaaat ggggacccga ggccgatttc cggcccggaa ccagtgccat
     3721 tgtgaagttc ggccgcaagc cggtcatcct gatccgcacg gagagcgggg agttccgcgc
     3781 gttcgacgcg acgtgcacgc acctggactg caccgtgcag taccgcgcgg accagcggga
     3841 catctggtgc gcctgccaca acggcgtgta cgatctccgg ggacagaacg tgtccggccc
     3901 gccgcccaga ccgcttggag cgtatgccgt caacatcgtg gacggcgacg tggtcatagc
     3961 ccggatggag gcctgaaccc atgtcgaagg gtccgaagcg tggcgtgttc gggtggctgg
     4021 acgatcggta cagcctgggt ccgctcgtca actacatgcg gcacaaggaa gtgccggtgc
     4081 accgccacac catctggtac tacatgggcg gggtcacgct cttcctgttc atcgtgcagc
     4141 tgttcacggg aatcctgctg ctcctgtatt accgtccggg cgcggagtcc gcgtacgaga
     4201 gtgtgcgctt cctgatgaac aaggtgccgt tcggatggct gttccgggcc gtgcacagct
     4261 ggggggcgaa cctgttcatc ctgttcgcct tcgtgcacat gttttcgacg tacttcacgc
     4321 gggcatacgc gaagccgcgc gagctgacgt gggtcaccgg gttcatcctg ctggccctgg
     4381 gcatgggatt cgggttcagc ggatacctgc ttccctggaa cgagctcgcc ttcttcgcga
     4441 cgaaggtcgg caccgacatc gccggcgtgc tgccgatcgt cggggacggg atcaagatct
     4501 tcctgcgggg aggcgacgac gtcaccgggg ccacgctgat ccggttctac gccttccaca
     4561 tcgcactcat tccggcgatc ttcacggcct tcctgggcat tcacctgctg ttcgtgcagc
     4621 ggcagggcat gcacgagccc gagtccgtcc ggctcctgcc ggatgggaaa cggagaatga
     4681 tggcattctt ccccaatttc gtgctccggg acctgttcgt gtggctgatc gtgctgaacg
     4741 tcctgctctt cctcgcggtg ttctacccgt gggagctggg gctcgaggcc gatccgttcg
     4801 cccccgcacc gccgggcatc aagcccgagt ggtacttcat gttcatgttc cagacgctga
     4861 agctggtccc cgcccatgtg ctgggcatcg agggagaggt gctcggcgtg ctcgtgttca
     4921 tggtggcggg gctcgcctgg atgctggttc ctttctggga gatccgctcc cggtccaccg
     4981 ggaagctcaa gcccatggcg gtgatcggat tccttgcgct cgcattcatc gtggtcatga
     5041 ccatagtcgg ctacctcgta tgacgattct catgagacgc cggcccggag gcgggctgtc
     5101 ggtatggctc gctgtcctgg cggccggttt cgcggcgctt cccgcggcgg cgcaggaaat
     5161 ccccgacgcg ctcagtggtg accagtgcgt ggcctgccac tcggacgtgg gctacctgcc
     5221 ggagggcatg atcctcgccg gcccgcacat gcgtgtcgag ctctcgtgcg caggatgcca
     5281 cggcggggac aacacgaccg acgacgagga catcgcgcat gccgggacgt tccgcggcgt
     5341 gccggacgag acggcgattc cggcgttctg cgggcgatgc cactccgacc tcggcttcat
     5401 gcgcgcgtac agcccgagcg cgtccaccga ccaggagtcg aactacttca ccagcgtcca
     5461 cggccagcgg ctgcgcgagg gggatacgaa ggtggcgcag tgcgcctcct gccacaccgc
     5521 gcacgcggtg ctgcccgcgt cggatgcccg gtccacggtg cacccgatga acgtgccctc
     5581 gctgtgcaac gagtgtcacg gcgacgcgga gtacatggcc gaatacggca tccggaccac
     5641 gcagtaccgc gactacgccc agagcgtcca cgggaccgcg ctcctcgagc gggaggacct
     5701 tggtgcaccc gcgtgcaacg actgtcacgg caaccacggc gcccagccgc ccggaatcga
     5761 gtcgatcgag cacgtctgcg gaatgtgtca cgtgaacaac gcggagttct tctcggcgac
     5821 cagcatggct cgagccttcg ccgaggatga gctgcacgcc tgcgaggagt gtcacgggat
     5881 ccacgacgtg cagaagacct tcgacgcgat ggtcgggacg ggcgacgact cggtctgcac
     5941 cgtgtgccac gaggaaggcg acgacggcta cgcggcggcg gaggccatct acgcccagct
     6001 cgtggccctg gtggccgccc aggactccgc ctcgacgctc gcggagcgcg tccgcgagat
     6061 cggcatggac gacatcgaga tcgggttcct gctgcgtgac gcccacgagt ccctgatcca
     6121 gtcccggacg ctggtccaca cgttcgatcc cgagcggacg gccgaggcga ccaccgtcgg
     6181 gatgggcaag gccaccgagg cgatcgagct cgggcacgcc gagatccgga acttccgctt
     6241 ccgccgcttc ggattcggca tcgcgacgct gctgatcacg gtcctgacgg tggcgctctt
     6301 cttcaagatc cgggacatgg agcggtcgta gccgggggcc gggtgtcgat ccgtgcgcaa
     6361 acccggcgac cgccgggccg ttctccgcgc gctgttcgcg ccgcggcatt gcttaaggat
     6421 gccctcggcg gtagactatc ggccctgaga cacctgaacg gcatgtcatg aagtggacag
     6481 agcgcgcccg cgcgaagtgg atcgagggga gggacacgtt ccgcagctgg ccgcggcgca
     6541 agcgcctggt cgtcggctgg gtcggagggg ccgtcgtgct cgtgctctcg atcgtggcct
     6601 ccgccgagta cacgtcgcag ccttcgttct gcccgacgtg ccactacatg gagccgtact
     6661 acgagtcgtg gcagacctcg ggcgcccacg ccgatatagc gtgcgcgacc tgccactttc
     6721 ccccgggcat cgcgggcacg atcaagggga aggccgaggg cctggtgcag gtcgtcaatt
     6781 acctggccag ctcctacacg cgccgcaagc cctgggccga gatcgacgat gtctcgtgca
     6841 tgcagagcgg atgccatgac acccgcctcg agggcgaggt gacgttcaag ggtgtcgcgt
     6901 tcgaccatgc gccgcacctg gagggactgc gccggggcaa gcagctccgg tgcacgagct
     6961 gccattcgca gatcgtccag ggcgatcaca tcacggtcac cgagactacg tgcttcctgt
     7021 gccacatgaa ggtcgggggc gaagtctc
//
LOCUS       DBNHQZ010000202         8249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1416906, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000202 DBNHQZ010000000
VERSION     DBNHQZ010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1416906"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1969)
                     /locus_tag="AC16YU_05375"
     CDS             complement(<1..1969)
                     /locus_tag="AC16YU_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR6064687.1"
                     /translation="MLRPHLLLVLAVLAAAPSAAQPFGDASDAVFRDDVVATVRVTVD
                     PLDFEAILAPENAQSDVEYLATFTFESPWVTATFDSVGLRLRGNTSRVSQKKSFRVSF
                     NTFIPGGDFRGLEKLNLNGEHNDPSIMRAKLSWDLFGTVAVPASRGTHVRLYINEEYF
                     GLYLNVEHVDERFLRRRFGHDDGFLYKCLWPADLTPPSEDACEVQLGADEFAAYAELG
                     DFLATINNTSDAAFPGAIERAFDVNGFLRHLAVTVATGSWDSYWFLKNNFYLFLDATD
                     GRYRFIPYDYDNTFGIWWDGIWSGLDWSTRDVYAWGHPVEPRPLATRILAVPAFRERY
                     TLYLRRLLSGGFAPEPLKARALDLRNLTAAAAEEDTYRTLDYGFTVQDWYDSYTIALA
                     DRNPTQQGHVTAGLLPFIDTRHASALAQLDAGNVAPVISEVRVTPLPARPVDPIEVTM
                     RVEDEGVPEVEVEYWPSGPTGLVATAAPAPDLGGDFWRAVLPALGTTGVLDVRVRARD
                     VEGATRTGSLVAVPVNFARPLLFLNELMALNATTVADEFGEFDDWVEIHNAGGADVSL
                     AGLFLTDDPGNPDRWALPDVTVPAGGFTLLWTDGTPEQGALHGPFRLGGGGETLALVD
                     ADLTTLDEVVFGAQTEDVAFGRTVDGAGSWQA"
     gene            complement(2058..5615)
                     /locus_tag="AC16YU_05380"
     CDS             complement(2058..5615)
                     /locus_tag="AC16YU_05380"
                     /inference="COORDINATES: protein motif:HMM:NF020337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR6064688.1"
                     /translation="MKSCKAGALAAAAVLMLSAEGVLARQVVISEVMASNVTSLHDED
                     GDTPDWVELYNPGPDAVPLGGYGLTDDPAAPFLWEFPAISIGAGDHLVVMASGKDRTA
                     LIAAWETVVTRGTSWRYLVGSSTPNVAWKGLGYNDTSWPTGASGFGYGDGDDATIVPT
                     GTITVYARKTFTVDDASQLVGARLHVDYDDAFVAYVNGVEVARANIGNPGEATSWDQT
                     ATRDREAQMFTGGDPVSFSLVNLDALFVTGDNVLAVEVHNVGSGSSDLSLIPFLTLAT
                     AAQEVSLAGVDPEIVHLFDAFSAHTNFALSDGETVCLTPPGGLGADCMEVPDGPSDIS
                     FGRDPADGSVGFFEYPTPGSANAPIAYGGQAPAVLMSHDAGFYAAPFTLSLSSPDPAG
                     RIAFTTDGSDPPVTATLSSATVPVNDTRVVRARLVVPGMLPGPIVTRTFIFEPTRTLP
                     VVSITTTPANLWDWETGIYVLGPNAEAANPHFGANYWQEWEIPVHMEWFEPGGSFGYG
                     VDAGMQIYGAWSRAHAQKSVALYARGRYGANAFEYPFFAERTLDSYQNILYRNSGNDW
                     NFSMLRDGFMQTLVHRTEIDRMAYRPTVQYLNGEYWGILNAREKINEHYVANIGNVEP
                     DAIDMLEFNVDRTVLHGDAAHWDALISYVTTSDMTAPEDLVEVERRMDLDNWIDYQIA
                     QIFFDNRDWPGNNQKMWRPRTEDGRWRWILFDTDFGFGIYNSSAYTLNTLEYAMEPNG
                     PAWPNPPGSTLLLRRLHRNAGFRALFVNRFADFLNTHFAVVRMEAVLDSLSGAIESEI
                     PLHTARWGAFSTTSWTNELAEMRTFARQRPAYMLGHVQAKFGVGSRSHVAVAVAEPGG
                     GTIRVNREHVGTGPWAGLYFVGIPVPVTAIPDAGYRFVGWTGSLESPSATVEVDPSGG
                     VSLFAHFEPGAAVSGDIVINEIQYNAAADVPSGDWIELTNVSLEEVDLSGWTFGDGAP
                     ANIFTVPDGAALAPGAYLVLCQDQPAFGLVHPQVSCLGSWTFGLSAGGESLFLRDASG
                     AIADSLTFMDEAPWPAEADGTGPTLELVDATSDNALPESWQAAFVRGGTPGARNSTSI
                     ANEPPTELPSGTPRVAAFPNPFAGRMSVTVEGPPTGEAVVEVFDALGRRIGRIELGRL
                     EPGPNTIVWSEASGAPGLRFVRLLVDGTPVASAKAIRID"
     gene            complement(5602..6282)
                     /locus_tag="AC16YU_05385"
     CDS             complement(5602..6282)
                     /locus_tag="AC16YU_05385"
                     /inference="COORDINATES: protein motif:HMM:NF027640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIR6064689.1"
                     /translation="MLQELRDIGLLQPIDAGEVLSSLIVALLCGIIVAWIYRRTYRGT
                     SYAPSFVRSMVVLPMITALVILVIGNNLARAFGLVGAMSIIRFRTAVKDIHDIVFIFF
                     SLTVGLAAGVGMHVAAWIGTFVIGLTVMATTGFGFGRISQNAMLLEFNYEVNGSSDPP
                     SYQGVLDRHCRFTSILSVRQPEEGDTLSLTFHIRLKRGADATRLVRELRDTERVSRVN
                     MYYDDEEL"
     gene            complement(6263..7069)
                     /locus_tag="AC16YU_05390"
     CDS             complement(6263..7069)
                     /locus_tag="AC16YU_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HIR6064690.1"
                     /translation="MRSEYKFLVPIDAAETVRRYVSRYAQYDRYALREPERAYTVRSI
                     YLDTAHGTIYDNKLEGLRDRIKVRIRGYGIVTPDSRVTLELKRKRGNGSWKSKASASL
                     VDVRDWLAGDDGVMPFAPDETTAARRFGYFARRFRLRPAALVVYDREAFVGVHDPTLR
                     VTMDMRLRGAFTDQLLDLGGPCPVSVYTRSFILEVKFDYHFPSWIKPLLEDLGAQKQA
                     LSKYVLTVDACAARSHRSWRSPFRGSLAYRRGLHQQTSELNGVPGAAGTA"
     gene            complement(7053..8147)
                     /locus_tag="AC16YU_05395"
     CDS             complement(7053..8147)
                     /locus_tag="AC16YU_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6064691.1"
                     /translation="MPISQRGSAGWLLLAALAGLAVVWFLPVEISPTVHLSAKMVAAS
                     EWTLRHDGQGTITAQLEERLAGVSGRYLVQSVDRGDTAELTLRPNLQAGVGVSEGDTI
                     GVLHSAATEFRWAEVQGLVSVSAAELAALAAGEKETMIQESRTRLDGLNAEIVVHRRI
                     VDRLRQLTESGAGSQSDLDAALARLVSLQGQAEVTAAQIAVLESGARDVDRNMAASQL
                     EAARRQLDALSLQRGALVVRVPLSGLLTLPPGDSVLVSVIDTTRWALIIPVPIAERAT
                     FTPGREVVLDADGSTARILHVNPTVQQVGGLSVVVAMAEGIGRPTEFLDRLTVSIRAE
                     GDPVRLRTWFAKEMKALFRWHNWFGRAERV"
BASE COUNT         1339 a         2831 c         2808 g         1271 t
ORIGIN      
        1 gcgcctgcca cgagcctgcc ccgtcgaccg tccggccgaa ggccacgtcc tcggtctgcg
       61 ccccgaacac gacctcgtcg agcgtcgtga gatcggcatc gacgagcgcg agggtctcgc
      121 cccctccacc cagccggaac ggaccgtgca gcgcaccctg ctccggcgtg ccgtccgtcc
      181 acagcagcgt gaagccgccg gccggaaccg tgacgtcggg cagcgcccag cggtccgggt
      241 tgcccgggtc gtcggtcagg aagagccccg cgagcgagac gtccgcgccg cccgcgttgt
      301 ggatctccac ccagtcgtcg aactcgccga actcgtcggc gacggtggtc gcgttcaggg
      361 ccatgagctc gttcaggaag agcagcggcc gggcgaagtt gacgggcacg gcaacgaggg
      421 agccggtcct cgtcgcgccc tcgacgtccc gggcgcgaac gcgcacgtcg aggactcccg
      481 tggtccccag ggcgggcagc accgcccgcc agaaatcccc gcccaggtcg ggcgccggcg
      541 cggcggtcgc cacgagcccg gtcggcccgc tcggccagta ttccacctcg acctcgggca
      601 cgccctcgtc ctcgacccgc atcgtgacct ctatgggatc gacgggccgc gcgggcagcg
      661 gcgtcacgcg gacctccgag atgacggggg cgacattgcc cgcgtcgagc tgcgcgaggg
      721 ccgacgcgtg ccgcgtgtcg atgaacggga ggagtccggc tgtcacgtgc ccctgctgcg
      781 tgggattgcg gtccgccagc gcgatcgtgt acgagtcgta ccagtcctgc acggtgaacc
      841 cgtagtcgag cgtccggtac gtgtcctcct cggcggccgc ggccgtcagg ttccgcaggt
      901 cgagcgcgcg ggccttcagc ggctccgggg cgaagccgcc cgagagcagg cgccggaggt
      961 acagcgtgta ccgctcgcgg aatgcgggga cggcgagtat gcgcgtggcc agcggacggg
     1021 gctccaccgg gtgtccccag gcatacacgt cgcgggtgga ccagtcgaga ccgctccaga
     1081 tgccgtccca ccagatgccg aaggtgttgt cgtagtcgta gggaatgaag cggtaccggc
     1141 cgtccgtggc gtcgaggaac aggtagaagt tgttcttgag gaaccagtac gagtcccagg
     1201 aaccggtcgc gacggtgacc gcgagatggc ggaggaaccc gttcacgtcg aacgcgcgct
     1261 cgatggctcc ggggaaggcc gcgtccgaag tgttgttgat cgtggcgaga aagtccccga
     1321 gttccgcata cgccgcgaat tcgtccgccc cgagctgcac ttcgcaggcg tcctcgctcg
     1381 ggggcgtcag gtcggccggc cataggcact tgtacaggaa cccgtcgtcg tgaccgaagc
     1441 gccggcgaag gaaccgttcg tcgacatgct ccacgttcag gtagagcccg aaatactcct
     1501 cgttgatgta cagtcggacg tgcgtgccgc gcgacgcggg gacggccacg gtcccgaaca
     1561 ggtcccagct gagcttcgcc cgcatgatgg acgggtcgtt gtgctcgccg ttcaggttca
     1621 gcttctccag gccgcggaag tcgccgccgg ggatgaacgt gttgaacgag acccggaaac
     1681 tcttcttctg cgacacgcgc gacgtgttgc cccgaaggcg gagacccacg ctgtcgaacg
     1741 tggccgtgac ccagggcgac tcgaatgtga atgtcgccag gtactcgacg tcgctctgcg
     1801 cattctcagg ggcgaggatg gcctcgaagt cgagcggatc gacggtgacc cgcaccgtgg
     1861 cgacgacgtc gtcccggaag accgcgtcgg aggcgtcgcc gaacggctgc gctgcggaag
     1921 gggccgcggc gagcacggcc aggacgagca ggagatgcgg tcgcagcatg aagacggaag
     1981 agtgtgaact ccaaacgtcc accggccccc cggggtccgg cgtcacggtc ccggcgccgg
     2041 gttccgggag tcccggctca gtcgatccgg atcgccttcg cgctcgcgac aggcgtcccg
     2101 tccacgagca ggcgcacgaa ccgcagcccc ggggcgccgg acgcctccga ccacactatc
     2161 gtgttgggcc cgggctcgag ccggcccagc tcgatccggc cgatgcgcct gccgagcgcg
     2221 tcgaagacct cgaccacggc ttcccccgtc ggggggcctt ccacggtcac cgacatgcgg
     2281 ccggcgaacg ggttcgggaa cgcggcgaca cggggcgtgc cggagggcag ctccgtcggc
     2341 ggctcgttcg cgatggacgt gctgttgcgc gcgccgggcg tgcctccccg gacgaacgcc
     2401 gcctgccagc tctccggcag ggcgttgtcc gaggtcgcgt cgacgagctc gagcgtcggg
     2461 ccggtgccgt cggcctcggc gggccacggg gcctcgtcca tgaaggtcag cgagtcggcg
     2521 atcgctccgg acgcgtcccg gaggaagagc gactcccctc cggccgaaag cccgaaggtc
     2581 cagcttccga ggcatgacac ctgcggatgc acgaggccga aggccggctg gtcctggcac
     2641 agcaccaggt acgccccggg tgccagcgcc gcgccgtcgg gcaccgtgaa tatgttggcg
     2701 ggcgccccgt caccgaacgt ccacccggag aggtccacct cttcgagtga cacgttcgtg
     2761 agctcgatcc agtctccgga aggcacgtcg gcggcggcgt tgtactggat ctcgttgatg
     2821 acgatatcgc ccgacaccgc cgcgcccggt tcaaaatgcg cgaagagcga tacgcccccc
     2881 gacgggtcca cctcgacggt ggcagacggg gactcgagcg atccagtcca gccgacaaaa
     2941 cggtagccgg catcgggaat cgccgtcacg ggcaccggta tgcccacgaa gtagaggccg
     3001 gcccacgggc ccgtcccgac gtgctcgcgg ttcacccgga tcgtcccgcc gccgggctcc
     3061 gccaccgcta ccgccacgtg gctccgcgat cccacgccga acttcgcctg tacatgcccc
     3121 agcatgtacg ccggccgctg cctggcgaag gttcgcatct ctgccagctc gttggtccac
     3181 gacgtcgtac tgaacgcgcc ccaccgcgcc gtgtggagcg ggatctccga ctcgatcgcg
     3241 ccgctcagcg agtccagcac ggcctccatg cggaccaccg cgaaatgcgt gttcaggaag
     3301 tccgcgaacc ggttcacgaa gagcgcccgg aagccggcat tgcggtgcag gcgacgaagc
     3361 agcagcgtgg acccgggcgg attgggccag gccgggccgt tcggctccat cgcgtactcc
     3421 agcgtgttga gcgtgtacgc cgaggaattg tagatgccga atccgaaatc ggtgtcgaac
     3481 aggatccacc gccatcggcc gtcctcggtc ctcggccgcc acatcttctg gttgttgccc
     3541 ggccagtcgc ggttgtcgaa gaagatctgc gcgatctggt agtcgatcca gttgtccagg
     3601 tccattcttc gctcgacctc gaccagatcc tccggagcgg tcatgtcgga cgtcgtcacg
     3661 taactgatca gcgcatccca gtgcgccgcg tcgccgtgca gaacggtccg atcgacgttg
     3721 aattccagca tgtcgatggc gtccggctcc acgttcccga tgttcgcgac gtagtgctcg
     3781 ttgatcttct cgcgcgcgtt caggatgccc cagtattcgc cgttcaggta ctgcaccgtg
     3841 ggacggtagg ccatgcggtc gatctcggtc cgatggacga gcgtctgcat gaagccgtcc
     3901 cgcagcatgg agaagttcca gtcgttgccc gaattgcggt acaggatgtt ctggtacgag
     3961 tcgagcgtgc gctccgcgaa gaacgggtac tcgaacgcgt tggccccgta gcggccgcgg
     4021 gcatagaggg cgacggactt ctgcgcgtgc gcccgggacc aggccccgta gatctgcatg
     4081 cccgcgtcca ctccgtaccc gaacgagccg ccgggctcga accactccat gtgcacgggg
     4141 atctcccact cctgccagta attggccccg aagtggggat tcgcggcctc ggcgttcggg
     4201 cccagcacgt aaatgccggt ctcccagtcc cagaggttgg ccggcgtcgt ggtgatcgac
     4261 acgaccggga gcgtgcgcgt cggctcgaaa atgaacgtcc gggtgacgat cggtcccggc
     4321 agcatgcccg gcacgacgag gcgggcgcgg accacgcggg tatcgttgac cggaaccgtc
     4381 gcggacgaga gggtggccgt gaccggagga tccgagccgt ccgtcgtgaa ggcgatgcgc
     4441 ccggcggggt cggggctcga gagcgacagc gtgaagggcg ccgcgtagaa cccggcgtcg
     4501 tggctcatca gtacggcggg cgcctgtccc ccgtaggcga tgggcgcgtt cgccgagccg
     4561 ggcgtcgggt actcgaagaa gccgaccgag ccgtccgcgg gatcgcgccc gaacgagatg
     4621 tcggacggcc cgtccggcac ctccatgcag tcggcgccca ggccgccggg cggcgtgagg
     4681 cagacggtct cgccgtcgga gagggcgaaa ttggtgtggg ccgagaacgc gtcgaacagg
     4741 tgcacgatct ccggatccac gccggcgagc gagacctcct gcgcggcggt cgccagggta
     4801 aggaacggga tcagcgagag atcgctcgat ccgctcccta cgttgtgcac ctcgaccgcg
     4861 agcacgttgt cgcccgtcac gaagagggca tccaggttca cgagggagaa cgagaccggg
     4921 tcgccgcccg tgaacatctg cgcctcgcgg tcgcgcgtcg ccgtctggtc ccaggacgtc
     4981 gcctcgccgg gattgccgat gttggcgcgg gcgacttcca cgccgttgac gtacgccacg
     5041 aaggcgtcgt cgtagtccac gtgaagccgg gcgcccacga gctgcgatgc gtcgtcgacc
     5101 gtgaacgtct tccgcgcgta gaccgtgatc gttccggtcg gaacgatggt cgcgtcgtcg
     5161 ccgtctccgt agccgaatcc gctcgcgccc gtcggccacg aggtgtcgtt gtagccgaga
     5221 cccttccagg ccacgttcgg agtggaggag ccgacgaggt atcgccacga cgtgccgcgg
     5281 gtgacgacgg tctcccacgc ggctatcagc gctgtccggt ccttgcccga cgccatgacg
     5341 accaggtggt cgcccgcccc gatcgagatc gccggaaact cccacaggaa tggagcggcg
     5401 gggtcgtccg tcagtccgta gcccccgagc ggcacggcgt ccggcccggg attgtagagc
     5461 tcgacccagt ccggcgtgtc cccgtcctcg tcatggaggg aagtgacgtt cgacgccatg
     5521 acctccgaga tcacgacctg gcgggccagc accccctctg ccgaaagcat caggaccgcc
     5581 gccgccgcga gcgctcccgc tttacagctc ttcatcgtca taatacatgt tgacccgcga
     5641 gacccgttcc gtatcccgaa gctcgcgtac cagcctcgtg gcgtcggcgc cccgcttgag
     5701 ccggatatga aaggtgagtg agagcgtgtc cccctcctcg ggctgccgca cgctcaggat
     5761 cgacgtgaac cggcagtgcc ggtcgagcac gccctggtac gagggcgggt cggaggatcc
     5821 gttcacctcg tagttgaact cgaggagcat cgcgttctgg ctgatccgcc cgaagccgaa
     5881 cccggtcgtc gccatgaccg tgagcccgat cacgaacgtc ccgatccagg cggcgacgtg
     5941 catcccgacg cccgcggcaa ggcccacggt gagcgagaag aagatgaaca cgatgtcgtg
     6001 gatgtccttc acggcggtcc ggaaccggat gatggacatt gccccgacca ggccgaaggc
     6061 ccgtgcgagg ttgttgccga tcacgaggat cacgagcgcc gtgatcatcg ggagcaccac
     6121 catcgaccgc acgaaggacg gcgcgtagct cgtcccccgg tacgtgcgcc ggtagatcca
     6181 ggccacgatg atgccgcaga gcagcgcgac gatgaggctc gagagaacct cgccggcgtc
     6241 gatcggctgc agcagcccta tgtcacgcag ttcctgcagc acccggtact ccgttcagtt
     6301 cgctggtctg ctggtgaagg ccccggcggt acgcgaggct gccgcggaac ggcgaccgcc
     6361 acgagcgatg gctgcgggcc gcacaggcgt ccaccgtgag cacgtacttg gacagcgcct
     6421 gcttctgggc cccgaggtcc tcgagcagcg gcttgatcca ggacgggaaa tggtagtcga
     6481 atttcacctc gagaatgaag gatcgcgtgt acaccgacac cggacacggt cccccgagat
     6541 ccaggagctg gtcggtgaac gcgccccgaa gccgcatgtc catcgtcacg cggagagtcg
     6601 gatcgtgcac gcccacgaac gcctcccggt cgtacacgac gagtgcggcc ggccgcaggc
     6661 ggaaacgccg ggcgaaatag ccgaagcgtc gggctgccgt cgtttcgtcg ggcgcgaacg
     6721 gcatgacgcc gtcgtcgccg gcgagccagt cccgaacgtc cacgagagac gcgctggcct
     6781 tcgatttcca cgagccgttg ccgcgcttgc gcttgagttc gagcgtcacc ctggaatccg
     6841 gagtcacgat gccgtatccg cgaatccgga ccttgatgcg gtccctgagt ccctcgagct
     6901 tgttgtcgta gatcgtgccg tgcgccgtgt ccaggtagat gctgcgcacg gtgtaggccc
     6961 gctcgggctc cctcagcgcg tacctgtcgt actgcgcgta ccgcgaaacg tatcgccgga
     7021 ccgtctccgc cgcgtcgatg gggacgagga atttatactc gctccgcacg gccgaaccag
     7081 ttgtgccagc ggaacagggc cttcatttcc ttggcgaacc acgtgcgcag ccgcacggga
     7141 tcgccctcgg ctcgaatcga gaccgtcagg cggtccagga actccgtggg ccgtccaata
     7201 ccctctgcca tcgccacgac cacgctcagt cctccgacct gttgtacggt cggattcacg
     7261 tgcaaaatcc gcgccgtact gccgtccgcg tcgagcacga cctcgcggcc cggggtgaac
     7321 gtcgcccgct ccgcgatggg caccgggatg atcagcgccc agcgcgtggt gtcgatcacg
     7381 ctcacgagca ccgagtcgcc gggcgggagg gtcaggagcc ccgagagcgg aacccggacc
     7441 acgagcgcgc cccgctgcag cgagagggcg tccagctgcc gcctcgcggc ctcgagctgg
     7501 ctcgctgcca tgttccggtc cacgtccctg gccccggatt ccagcacggc gatctgggcg
     7561 gcggtcacct ccgcctggcc ctggagggag accaggcgtg cgagcgccgc gtccaggtcg
     7621 ctctgcgatc cggcgcccga ctcggtcagc tgtctgagac gatcgacgat ccgacggtgt
     7681 acgacgatct cggcgttcag gccatcgagc ctggtccgcg attcctggat catcgtctcc
     7741 ttctctcccg cggccagggc ggccagctcg gccgccgaca ccgatacgag cccctggacc
     7801 tccgcccacc ggaactcggt ggctgcggag tgcagcacgc cgatagtgtc gccctcgctg
     7861 acgcccaccc cggcctggag gttcgggcga agggtgagtt cggccgtgtc gccccggtcc
     7921 accgactgga ccaggtaccg cccggagacg ccggccaggc gctcctccag ctgggccgtg
     7981 atggtgccct ggccgtcgtg cctcagcgtc cactccgagg cggcgaccat ctttgccgac
     8041 aggtgcacag taggcgaaat ctctacgggc agaaaccaca cgacggccag ccctgccagg
     8101 gcggccagca ggagccaacc ggctgaacct ctttgggaga tcggcacggg cctgtctgcg
     8161 ccgctgcgcg gcgcgttgcg tccgggttac caaatcgtaa ccaagataga cccgtttgcc
     8221 tgaagctcag caccgggcgc agggggatc
//
LOCUS       DBNHQZ010000203         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1282665, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000203 DBNHQZ010000000
VERSION     DBNHQZ010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1282665"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /locus_tag="AC16YU_05400"
     CDS             <1..2429
                     /locus_tag="AC16YU_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024207.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6064692.1"
                     /translation="SEGILSDRQADSLLSILPDTAPSGPLDPESRWADPRPPSVLHGA
                     PDSGEGIDDPAHFWRTFGFAHASGRYAELAPDPHRTVAAMPIRAGWAPADSGRPPALP
                     RLVPDLCTGCGNCWTVCPESAILPVAPSFQAVLDTAFARLAAAGGEAFQLGRVSKPLV
                     QEAYRIFRDDALGQYRTLDRLVAEALERVMTRANLDEEKRSLLSAEFEALSHHLPALG
                     LARTEPWFDAAESTAKGSGRALLLAVDPDRCTACGACVAACPESALEPVTTDDPTLAA
                     ERLGAAFATALPPLPADVIDAAASVEPASRGLDHRAPGAAAGGDDAPLRRASIRAVAI
                     AAEALRGPAIDRLTERVRAAREALEAAIRAEVSGTVSVNDFEAFSKRLATLGDAPSAD
                     RLAAAAAGEGTVDADRLQRLADTRNETAALEERLTRGEGRARIVAVLEGQVRPTWPLN
                     PYGFPWIRVAQGSGPAVVRAIADDLLERHLREIALVRHAERLAGNMTDAARPPAVDPA
                     SVTGDEALAWPVPLLITDHLSAGLVALLEGKRPVRVALLRSDPTDPDAGDPAIAAAAL
                     GSACVVQGSLGDVQHLVAGLEAALTSREPSLISLHAPTGPATDALARARDAILARIAP
                     LLVYRPATSGLPGGTLDLSGNPSPDEDFAVADGSVVTPAHWIAREPAFRTEFLWVARG
                     NEPSGSTEVSRLLDGSVDEGVPIIADPRGHVAVVPPRHLLAAGRRLKAWRRLRAMAAS
                     TAPAVPATPPAAPAPPVAPAPAPEPAVPADVHARLTARLLALSGFAPGLAAGQQPLRT
                     LVSDPTPDEA"
     gene            2434..>3367
                     /locus_tag="AC16YU_05405"
     CDS             2434..>3367
                     /locus_tag="AC16YU_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HIR6064693.1"
                     /translation="MWSLDFEKRTFRHGIHPADYKAVTRSLAIERMPFTDSVVLPLNQ
                     HLGAPSKAIVQVGDHVQRGQMIAEAGGYVSVPLHASVSGLVKSIELRHHASGQMVEAI
                     EIERDPTSPQTLFGERTLDLDTMSPEEIITAVQAGGFVGLGGAAFPTHVKLSPPEGKH
                     ARFFMVNAAECEPYLNSDFRILLERTESIIHGIRVLMKALGSEKVYIGTEDNKIDAAD
                     HLRAALPPDLPIEVLVLQTKYPQGAEKMLVDAVLDREVPSGKLPIDQEVVVQNVGTVA
                     GIGDMFRWGQPLIERVVTVTGPGIREPKNLLVPIG"
BASE COUNT          441 a         1316 c         1151 g          459 t
ORIGIN      
        1 actctgaggg catcctcagc gaccggcagg ccgactccct gctctccatc ctccccgata
       61 cggcgccctc cggacctctc gatcccgagt cgcgctgggc cgatccccga ccgccgtccg
      121 tgctgcacgg cgccccggac tccggggagg gcatcgacga tcccgcgcat ttctggcgga
      181 ccttcggttt cgcccacgcc tccggccgct acgccgagct cgctccggac ccgcaccgca
      241 cggtggccgc catgccgatc cgcgcgggct gggcgcccgc cgactccggc cggccccctg
      301 ccctgccgcg gctcgtcccc gacctgtgca cgggatgcgg caactgctgg accgtctgcc
      361 ccgagagcgc catcctcccc gtcgcgccca gtttccaggc cgtgctcgac acggccttcg
      421 cccggctcgc cgcggccgga ggcgaggcgt tccagctcgg tcgcgtctcg aagcccctcg
      481 tgcaggaagc gtaccggatc ttccgggacg atgcgctggg gcagtaccgg accctggacc
      541 gcctggtggc cgaggcgctc gagcgcgtca tgacccgggc gaatctggac gaggagaagc
      601 gctcgctgct ctccgcggag ttcgaggccc tttcgcacca ccttccggct ctcgggctcg
      661 cccggacgga gccgtggttc gatgccgccg agtcgacggc gaaggggtcg gggcgagcgc
      721 tcctcctcgc cgtggacccg gaccgctgca ccgcctgcgg cgcgtgcgtc gccgcgtgcc
      781 ccgaatcggc cctcgaaccg gtcacgaccg acgacccgac gctcgcggcg gaacgcctgg
      841 gcgccgcctt cgcgaccgcg cttccgccgc tgccggccga tgtcatcgac gcggccgcgt
      901 ccgtcgagcc ggcatcccgc ggcctggacc accgggcgcc gggcgcggcg gccggcgggg
      961 acgacgcccc ccttagaagg gcgagcatcc gcgcggtggc catcgccgcc gaggcgctgc
     1021 gcggtcccgc gatcgaccgg ctcacggaac gggttcgggc ggcccgcgag gcgctcgagg
     1081 ccgcgatccg cgccgaggtg tccggcacgg tgagcgtgaa cgacttcgag gccttctcga
     1141 agcgactcgc aacgctcggc gacgcgccgt ccgccgaccg cctggccgcg gccgccgccg
     1201 gggaaggcac cgtcgacgcg gatcgcctgc agcgcctcgc cgatacgcgg aacgagacgg
     1261 cggccctcga ggagcggctc acccggggag agggccgcgc gcgcatcgta gccgtcctcg
     1321 aggggcaggt ccgaccgacc tggccgctca acccgtacgg attcccgtgg atccgcgtgg
     1381 cgcagggctc gggccccgcc gtggtgcggg cgatcgcgga cgatctgctc gagcgccacc
     1441 tgcgggagat cgccctcgtc cgccacgccg agcgcctggc cgggaacatg accgatgccg
     1501 cccggccacc cgccgtggac cccgcttcgg tcaccggcga cgaggcgctg gcctggcccg
     1561 tgccgctgct catcaccgac cacctctcgg ccggactcgt cgcgctcctg gaagggaaac
     1621 gccccgtgcg cgtcgccctc ctgcgttcgg atccgaccga tcctgacgcc ggcgaccccg
     1681 cgatcgcggc cgccgcgctc ggatccgcct gcgtcgtgca gggcagcctg ggcgacgtgc
     1741 agcatctcgt ggccgggctc gaggcggcgc tcacgtcgcg cgagccctcc ctgatcagcc
     1801 tgcacgctcc gaccggcccc gcaacggacg ccctcgcgcg ggcccgcgac gccatccttg
     1861 cacggatcgc gccgctcctg gtctaccggc cggcgacgtc cggccttccg ggaggcacgc
     1921 tggacctgtc cggcaacccg tccccggacg aggacttcgc ggtcgcagac ggctccgtgg
     1981 tcaccccggc gcactggatc gcccgcgagc ccgcgttccg cacggagttc ctttgggtcg
     2041 cccgcggcaa cgagccttcg ggctcgaccg aagtctcgcg cctgctcgac ggctcggtgg
     2101 acgagggcgt cccgatcatc gcggatccgc gaggccacgt cgccgtcgtg ccgccccgcc
     2161 acctgctggc agccggtcgc cggctcaagg cgtggcgaag gctccgcgcg atggccgcct
     2221 cgaccgctcc ggccgtcccg gcgacgcccc cggcggcacc cgcccccccg gtcgcccccg
     2281 ctcccgcacc cgagccggcc gtcccggccg acgtgcatgc gcggctcacg gcccggctcc
     2341 tcgccctcag cggtttcgcc ccgggtctcg cagccgggca gcagccgctc cgtacgctgg
     2401 tctccgatcc cactccggac gaggcctgat cccatgtgga gcctcgattt cgagaagcgc
     2461 accttccggc acgggatcca cccggcggac tacaaggccg tgacgcgcag cctggcgatc
     2521 gagcggatgc cgttcaccga ctcggtcgtc ctgccgctca accagcacct cggggccccg
     2581 tcgaaggcga tcgtccaagt cggagaccac gtgcagcggg gacagatgat cgcggaagcg
     2641 ggcgggtacg tgtcggtccc gctccatgcc tccgtatccg gcctcgtaaa gtccatcgag
     2701 ctccggcacc acgcctccgg gcagatggtc gaggccatcg agatcgagcg cgacccgacc
     2761 tcgccccaga ccctgttcgg cgagcgtacg ctggacctgg acacgatgtc gccggaggag
     2821 atcatcaccg cggtgcaggc gggcggcttc gtgggcctgg gcggcgcggc tttccccacg
     2881 cacgtgaagc tctcgccccc ggagggaaag cacgcgcgct tcttcatggt gaacgcggcc
     2941 gagtgcgagc cctacctgaa ctcggacttc cggatccttc tcgagcgcac cgagagcatc
     3001 atccacggaa tccgtgtcct gatgaaggcg ctcggctcgg agaaggtgta catcggcacc
     3061 gaggacaaca agatcgacgc ggccgaccat ctcagggcgg cgctgccgcc ggacctgccc
     3121 atcgaggtcc tggtcctcca gaccaagtat ccccagggcg ctgagaagat gctcgtcgac
     3181 gcggtgctcg accgggaggt gccgtcgggc aagctcccga tcgaccagga ggtcgtcgtg
     3241 cagaacgtcg gcaccgtggc gggcatcggg gacatgttcc gctgggggca gccgctcatc
     3301 gagcgggtgg tgaccgtcac gggccccggt atccgggagc cgaagaacct cctggtgccg
     3361 atcggca
//
LOCUS       DBNHQZ010000204         5358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_61269, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000204 DBNHQZ010000000
VERSION     DBNHQZ010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_61269"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16YU_05410"
     CDS             complement(<1..328)
                     /locus_tag="AC16YU_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064694.1"
                     /translation="MSDAARSDIAVTQTAFIRNHPARYRQVSGEGLQEIVLETLPLES
                     GRVLSEVPVAYRTFGKLDPDGSNAVVVCHALTGDTAVDQWWSELVGPGRVLDTDRLFV
                     VCANVLG"
     gene            complement(321..1643)
                     /locus_tag="AC16YU_05415"
     CDS             complement(321..1643)
                     /locus_tag="AC16YU_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR6064695.1"
                     /translation="MSHSDTPLRFETLQLHAGQEADPTTGSRAVPIYATTSYAFESTE
                     HGADLFALRRFGNIYTRIMNPTTDVFEKRIAALEGGVAALATASGQAAQLLALTTLAE
                     AGDNIISTSYLYGGTYNQFKVAFPRLGIDVRFVDGDDPADFERLIDERTKALYVETIG
                     NPRFNVPDFEALAAIANRHGVPLVVDNTFGAAGYLCRPLEHGAHIVVESATKWIGGHG
                     TTIGGVIVDGGTFPWDNGRFPTFTTPSPGYHGLNFWETFGPNGVLGVNVAFIIRARVE
                     GLRDLGPSQNPFGSFLLLQGLETLSLRVERACENALELANWLRGRDEVAWISYPGLVD
                     HPSHDRAAKYLANGFGSVLAFGVNGGLEAGQAFVNNVKLASHVANVGDAKTLVIHPAS
                     TTHQQLSDDEQRASGVTPDLVRVSVGIEHIDDIRNDFAQALASVPQYV"
     regulatory      complement(1801..1925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2139..>5358
                     /locus_tag="AC16YU_05420"
     CDS             2139..>5358
                     /locus_tag="AC16YU_05420"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HIR6064696.1"
                     /translation="MILHRFACAALIAGLACLTPGPARASRQVPAEDPSIAWQAPGRT
                     VRFESLTIEDGFMPAVATVIMQDRTGFLWFGTGSGLSRYDGYEFRTYKHDPDDPRSLG
                     HNQVMGLLEDRDGSIWVGTFGGGLSRFDPATGAFTRFRHDPDDLRSLSHDFVGPVYQD
                     TEDVLWIGTEGGGLNRFDPATGEFSRFRHDPDDPGSLCDSTNAVRAIYEDRRGALWIG
                     TWGGGLCMLDRESGRFTPFPLNASGAPRTVLAFHEDREGRLWAATEHAGLLRVERDLG
                     TVSPVEIDGHRMLAGTPIGAIREDGEGSLWLGTWGRGLCRFDSGDATLMCFRQNPYDP
                     HSLADDGVSAVHIDRSGVLWASTWGGLCRVDLRTVGVSRFRYAPNDPGSLSSRDVRAL
                     HVDRSGAVWAGTWNGGLNRRDRDSGQFEHYGYLPGDRRGLSNANVQAILEDRTGAIWI
                     GTWGGGLNRFDRASGTFQRFRHDPTDPGSLHSDSVLEIYEDRDGVLWIGTWDGLSRRD
                     PATGRFRRVPNESQSAGGPGHVEVIAVLRDSEGILWVGTRDDGLGRLDPQQGTLTFST
                     HDSDDPASLGHNTVWAVHEDRGGALWVATGGGGLNRYDRENGRFTRYTTHNTALGDNV
                     IYAIVEDGSGTLWLNTERGLARFEPATARVTTYAARSDLGDCGFNTAAFHDHGSGTLY
                     IGCSDGFVSFRPEQLRENPDPPAVVLTDIRVFGRFVGREAERPSGASVVGGRELVLAH
                     DENDLSISFVALHYANPSHNQYAFMLENYDTDWRQAGTQRTATYTHLDPGAYTFRVRA
                     ANSDGVWTEEGASLRVVIRSPWWLTTPAWLAYGVLLVALVVAVNRFQRRRIVRHEREA
                     ALIREKDLRAEAAEARASFLHLENERKTQELEQARRVQLAMLPESVPVHPLVSVAAGM
                     QTAAEVGGDYYDFDVAPDGSLTFVIGDATGHGVQAGIMVTAMKSLWSVFAREQDLLDL
                     VARTSEALRRMGMPRLYMALAVGRLGGRTLELVGVGMPPAVVWRADSGCVEHVALKGM
                     PLAGRLTFPYRREVVVLRPGDVVVLMSDGFPELADESGEMVGY"
BASE COUNT          822 a         1769 c         1916 g          851 t
ORIGIN      
        1 aaccgaggac gttcgcgcag accacgaaga gccggtccgt gtcgaggacc ctgcccgggc
       61 ccaccagctc ggaccaccac tggtccaccg ccgtatcgcc ggtcagggcg tggcagacga
      121 ccacggcgtt cgagccgtcc ggatccagct ttccgaaggt gcggtacgcc accggcacct
      181 cgctcagcac gcggccgctt tccaggggaa gggtctccag gacgatctcc tggagccctt
      241 cccccgaaac ctggcgatac cgggccgggt ggttgcggat gaaggcggtc tgcgtcacgg
      301 cgatgtcgga gcgggctgcg tcagacatac tggggcacgg aggcgagcgc ctgcgcgaag
      361 tcgttcctga tgtcgtcgat gtgctcgata ccgaccgaga cgcggaccag gtccggcgtc
      421 acgcccgagg ccctctgctc gtcgtccgac agctgctggt gcgtggtcga ggccgggtgg
      481 atcacgaggg tcttcgcgtc gcccacgttg gccacgtggc tcgccagctt cacgttgttg
      541 acgaatgcct gcccggcctc gagtccgccg ttcacgccga aggcgagcac ggatccgaag
      601 ccgttcgcga gatacttcgc cgcccggtcg tgcgacgggt ggtcgacgag ccccgggtag
      661 ctgatccagg ccacttcatc gcggccgcgc agccagttgg cgagctcgag cgcgttctca
      721 caggcacgct ccacgcggag cgagagcgtt tcgaggccct ggagcagcag gaacgagccg
      781 aacgggttct ggctgggccc caggtcgcgc aggccctcga cgcgcgcacg gatgatgaaa
      841 gccacgttga cgcccagcac gccgttcggg ccgaacgtct cccagaagtt caggccgtgg
      901 tacccgggcg agggcgtggt gaaggtgggg aaacggccgt tgtcccaggg gaaggtcccg
      961 ccgtccacga tcacgccgcc gatcgtcgtg ccgtgcccgc cgatccactt cgtcgcgctc
     1021 tcgaccacga tgtgcgcgcc gtgctcgagc ggtcggcaca ggtatccggc ggcgccgaag
     1081 gtgttgtcga cgacgagcgg cacgccgtgc cggttggcga tggcggcgag cgcctcgaag
     1141 tcgggaacgt tgaagcgcgg attgccgatc gtctcgacgt agagggcctt cgtgcgctcg
     1201 tcgatgagcc gctcgaagtc ggccgggtcg tcgccgtcca cgaagcggac gtcgatgccc
     1261 aggcgcggga aggcgacctt gaactggttg taggtgcctc cgtagaggta gctcgtcgag
     1321 atgatgttgt cgcccgcctc ggccagcgtg gtgagcgcca ggagctgggc ggcctggccg
     1381 ctggcggtcg cgagcgcggc gacgccgcct tcgagcgcgg cgatccgctt ctcgaagacg
     1441 tccgtcgtcg ggttcatgat gcgcgtgtag atgttgccga accggcggag ggcgaacagg
     1501 tcggcgccgt gctcggtcga ctcgaaggcg tacgacgtcg tggcgtagat cgggacggca
     1561 cgcgacccgg tggtgggatc ggcctcctgg ccggcgtgca gctgaagcgt ctcgaagcgg
     1621 agaggcgtgt cggagtggct catggtcttc ggaatgagga tggtgtccgg gtgtggcact
     1681 catcccgaag agcgggggag cggagtccgc tccccgcgac aggggcatca aaaaacccct
     1741 ctcgcggaga tggcgagaga ggtcgaacac gaatccgcga gcggaccggc acgggtctcc
     1801 cgctcatctt tccccgcgca ttgcgaggca ggaattggca ccttccgaac gtctgtctct
     1861 gggagcgacg ccaggggttg ccagggcttc gacgggcctg caccctccgc ccttctggat
     1921 gagctggtct gagtatacga attcgctacc ggcgaaatca agaggcggcc tcgcaaacgt
     1981 ctcggggaat cgactacggg ggccatcgtg aaatccccgt gcgtcgtatc ctcagacgac
     2041 tgcattcgta accagcgtgt cgtccgttcc gggcggcaca tcgcccttat cggccggcca
     2101 cgctgacctc ccgtcaccgg catcgggctc ccatgagcgt gatcctccac cgattcgcct
     2161 gcgccgccct gatcgccggc ttggcgtgcc tgacccccgg cccggcccgg gcgtcacgcc
     2221 aggtgccggc cgaggatccg tccatcgcat ggcaggctcc gggccgcacg gtgcgtttcg
     2281 agtcgctgac catcgaggac ggtttcatgc cggccgtggc gacggtcatc atgcaggacc
     2341 gcacgggctt cctgtggttc gggaccggga gcggcctcag tcgctacgac ggatacgagt
     2401 tccgcacgta caagcacgat ccggacgatc ctcgcagcct cggtcacaac caggtcatgg
     2461 ggcttctcga ggaccgggac gggtcgatct gggtgggcac gttcggcggg ggcctcagcc
     2521 ggttcgatcc ggcgaccggg gcgttcacgc ggttcaggca cgacccggat gatctccgga
     2581 gcctgagcca cgatttcgtc gggcccgtct accaggacac ggaggacgtg ctctggatcg
     2641 ggacggaagg aggaggactg aaccggttcg atccggcgac cggcgagttc tcccggttca
     2701 ggcacgatcc ggacgatcct ggcagcctgt gcgactcgac gaacgccgtt cgcgcgatct
     2761 acgaggaccg gcggggcgca ctctggatcg ggacgtgggg cggcggcctg tgcatgctcg
     2821 accgggagtc ggggcggttc acgccgtttc cgctcaacgc ttccggcgcg ccgcggaccg
     2881 ttctggcctt ccacgaggac cgggagggac ggctgtgggc ggcgaccgag cacgccggcc
     2941 tgctgcgggt ggagcgggat ctcggaaccg tgtcgcccgt ggagatcgac ggtcaccgga
     3001 tgctggccgg gacgcccatc ggcgcgatcc gagaagacgg cgagggctcg ctctggcttg
     3061 gcacgtgggg cagggggctc tgccggttcg actccggaga tgcaacgctc atgtgcttcc
     3121 ggcagaatcc ctacgatccg cacagcctgg ccgacgacgg ggtgagcgcc gtgcacatcg
     3181 atcgctccgg ggtcctctgg gccagcacgt ggggaggcct ctgccgcgtg gatctccgta
     3241 cggtgggcgt atcgcgtttc cgctacgcac ccaatgatcc gggcagtctc agcagcaggg
     3301 acgtccgcgc gctccatgtc gatcggtcgg gcgcggtctg ggccggcaca tggaacggcg
     3361 gtctcaaccg gagggaccgc gactccgggc agttcgagca ctacgggtac ctgcccggcg
     3421 accggcgggg cctgagcaac gccaatgtcc aggccatcct cgaggaccgt accggcgcga
     3481 tctggatcgg cacgtggggc ggcggcctca accggttcga ccgtgcgagc gggaccttcc
     3541 agcgcttccg gcacgacccg accgatccgg gcagcctgca cagcgactcg gtactggaga
     3601 tctacgagga ccgtgacggc gtgctctgga tcggtacctg ggacggcctc tcccgccggg
     3661 atcccgcgac ggggaggttt cggcgcgtcc ccaacgagtc gcagtcggcg ggcggccccg
     3721 gccacgtgga ggtcatcgcc gtcctcaggg attccgaggg gatcctgtgg gtcggcacgc
     3781 gggacgacgg gctgggtcgc ctggatccgc agcagggaac gttgaccttt tcgacccacg
     3841 attcggacga tcccgcgagt ctcggccaca acaccgtctg ggcagtccac gaagaccgcg
     3901 ggggcgccct atgggtggcc acgggtgggg gcgggctgaa ccggtacgac cgggagaacg
     3961 gccgcttcac ccggtacact acgcacaaca ccgcgctcgg ggacaacgtg atatacgcaa
     4021 tcgtcgagga cgggagcggg acgctctggc tcaacaccga gagagggctc gcccgcttcg
     4081 agccggccac ggcacgcgtc accacctacg ctgcccgctc cgatctcggg gactgcggct
     4141 tcaacacggc cgccttccac gaccatggca gcggcaccct gtacataggc tgttcggacg
     4201 gcttcgtgtc gttccggccc gagcagctcc gcgagaatcc cgacccgccg gccgtggtcc
     4261 tgacggacat ccgggtgttc ggacgattcg tgggacggga ggccgagcgc ccctccggag
     4321 catccgtggt cggcggccgc gagctcgtgc tcgcccacga cgagaacgac ctgtcgatct
     4381 ccttcgtcgc cctgcactac gccaacccgt cgcacaacca gtatgccttc atgctggaga
     4441 actacgacac cgactggcgc caggcgggca ctcagcgtac ggccacgtac acccacctcg
     4501 atccaggggc ctacacgttc cgggtccggg cagccaacag cgacggggtg tggacggagg
     4561 aaggcgcgtc actgcgcgtg gtgatccgct caccgtggtg gctcacgacg ccggcatggc
     4621 tcgcgtacgg ggtgctcctg gtcgccctcg tcgtggcggt gaaccgcttc cagcgacggc
     4681 gcatcgtccg gcatgagcgc gaggccgcgc tgatccgtga gaaggacctt cgcgcggagg
     4741 ccgccgaggc ccgggcgtcg ttccttcacc tggagaacga gcgcaagacc caggagctcg
     4801 agcaggcacg gcgcgtgcag ctggcgatgc tgccggagag cgtgccggtc catcccctgg
     4861 tctcggtcgc ggccggcatg cagacggcgg ccgaggtggg cggggactac tacgatttcg
     4921 acgtcgcgcc ggacggttcg ctcacgttcg tgataggtga cgccaccggg cacggtgtgc
     4981 aggccggcat catggtcacg gccatgaaga gcctctggag cgtgtttgca cgggagcagg
     5041 acctgctcga cctcgtcgcc cggaccagcg aagccctgcg gcgcatgggg atgccccggc
     5101 tgtacatggc cctcgccgtg ggacgtctcg gaggccgcac cctggagctc gtgggtgtcg
     5161 gtatgccgcc ggcggtcgtg tggcgggctg attccgggtg cgtggagcac gtcgcgctca
     5221 aggggatgcc gctcgcaggc cgcctcacgt tcccgtaccg gcgcgaggtc gtcgtgctgc
     5281 ggcccggcga cgtcgtcgtt ctcatgagcg acggcttccc cgaactcgcc gacgagtcag
     5341 gcgagatggt cgggtacg
//
LOCUS       DBNHQZ010000205         5910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3314972, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000205 DBNHQZ010000000
VERSION     DBNHQZ010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3314972"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="AC16YU_05425"
     CDS             <1..1020
                     /locus_tag="AC16YU_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6064697.1"
                     /translation="LVRAAIGEDVDQETLGGAATHSRISGVTDYEMPDDATCLATIRD
                     LVSRLGSRPTAGFDRALPVEPAFPTDDLFGIVPVEGHAAFDTHEVLARLIDADSWTEY
                     KAGYGQTLLTGYARIDGWSVGIVANQRKIVRARAGRKAHTDEMQAGGVIYSDSADKAA
                     RFIMVCNQKRIPLVFLQDVTGFMVGTRAEQGGIIKDGAKMVNAVANSVVPKFTVVTGN
                     SFGAGNYAMCGRAYDPRLMLAWPTARIAVMGGNQAAETLLQIHVAKLSRQGMEPDRLQ
                     QDELLRSIRARYEETTSPYYAAARMWIDEIIDPRLTRRWISTGIEMADHNPDMEPFRT
                     GAIQT"
     gene            1027..2883
                     /locus_tag="AC16YU_05430"
     CDS             1027..2883
                     /locus_tag="AC16YU_05430"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6064698.1"
                     /translation="MSFRSTPRRFHDWVRVRPDAPAYYERSGGRWVPTDWKTYTGEVR
                     AAAKALMALGFEPGTMVAIMGFNAPSWTISDLAAMTAGGTPVGVYANSSLDQVGFILG
                     HSGARFLVIDGMDAWRRVRSEVERLPDLEHVVFMRGAEASDDPRVLSWDRFLAHGTRV
                     AEADLDRRMDGLRPEDVGALIYTSGTTGPPKGSMLSHGALAAATDMGIELLPDPHIGA
                     RLLSYLPMAHAAERALTLLGPASYGYTVYYAESIDAMPANLREVEPEIFLGVPRVWEK
                     IRAGVEERIGAIRGPRRRLIAWARSVALSVHEERCRGMELRGSLARDYRWAKRLVLDR
                     IRTAVGLGRSRVLLSGAAPMAEEDLRALASLDMPVREVYGQTEDCGPTTNNRPGETRW
                     GSVGRPFRGCEVKIADDGEVLVRGPHLFSGYFRDPEASAEALRDGWLHSGDLGFVDTD
                     GYLFITGRKKEIIITSGGKNVAPRNLEEAIRESPLVEDVVLVGDARKHLGALVTLRPG
                     AVRSLQEESGPSGTVPAHTDRIVLTAIWEHVERVNDRFSRLEHVRRIMVLPGLFSVRS
                     GELTPTLKVRRETIARHYAKEIDLLYSGTPIDGTGVVPAPDEPPQPGSSEPT"
     gene            2890..3336
                     /locus_tag="AC16YU_05435"
     CDS             2890..3336
                     /locus_tag="AC16YU_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HIR6064699.1"
                     /translation="MARDKPIWFRPLTRKALNAMCKDTLMEALGIEFVEVGPDFLAAR
                     MAVDRRTRQPMGLLHGGASLALAESVCGAAASASVDADRFACYGMEINANHVRTAKGG
                     EVVCTARPAYLGATTQVWQFEIRNASGDLLCTGRQTVAVRPVVLPE"
     gene            3433..>5910
                     /locus_tag="AC16YU_05440"
     CDS             3433..>5910
                     /locus_tag="AC16YU_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6064700.1"
                     /translation="MNESDEAEGAGRPATRRRAPFDAERAGRLARDVWGLEARATELV
                     GDMDRNFRLETADGTVVALKISRVGRDHLPLSVRQAAVDAVATRTGAPRVPRSVPASD
                     GRTVVSIEGPGGAVFEAACTEWVEGTPLVDFRPRTLALCRDLGQGLAMVGSALESTRH
                     DALDRASDWDLRRGFAVAERHAGAVDPGRRGLFDRTLRRAGEAAGDWDRLALGVIHGD
                     ANDHNVLILLDGAVPVPGLIDFGDMIRSWRAAEPAIAAAYAAMDQPDPVGVIAAVAAG
                     FDAGMPLDEHELDALPQLVALRLCVSVALSAVRSAREPDDPYLTVSERPAWDLLERLD
                     AVHPRLAAARIRAACGREPSPRGAAVSSWLRSDAADPAPVLAAAGPVAVLDLSVGGAD
                     AAGLRDPARWSETVRRILDDAGASIGIGRYLEPRLCYGAEQFEAGEREPEFQRTVHLG
                     VDLFAPPGTRVRAPFDGRVAAVADNAHPLDYGPTVILHHGLDGGGPEFWTLYGHLDPD
                     VLEHLAPGRHVARGEAFARIGREDRNGGWPPHVHFQVIADRLDRTDGDFHGVALPSET
                     VAWAALCPEPPIGFAVDARYREEPDERLAARRRARIAPSLGLSYSRPVHMVRGSGTWM
                     FDERGRRYLDCVNNVNHVGHAHPRVVDAAMRQMELLNTNTRYLHGNLVRYAERLTATL
                     PAPLDTCILVNSGSEANDLAFRIARSATGRRGIVVVDGAYHGHLTSLIDASPYKFDGP
                     GGSGRAEHVRVVRMPDAYRGEIRGDDPDVGPSYADDVREAAASLATSGLPAAAFIAES
                     ILSCGGQIVPPEGWLRSAAAHARAAGAL"
BASE COUNT          862 a         2056 c         2172 g          820 t
ORIGIN      
        1 ctggtgcggg cggccattgg cgaggatgtg gaccaggaga cgctgggcgg tgcggcgacg
       61 cactcccgga tatcgggcgt gaccgactac gagatgccgg acgacgcgac gtgcctggcg
      121 accatacgcg acctggtctc ccgcctcggc agccggccga cggcgggctt cgaccgcgcc
      181 ctccccgtcg agcctgcctt tccgaccgat gacctgttcg ggatcgttcc cgtcgagggg
      241 cacgcggcgt tcgacaccca cgaggtgctc gcgcggctca tcgacgccga ctcctggacc
      301 gaatacaagg ccgggtacgg gcagaccctg ctgaccggat acgcccggat cgacggatgg
      361 agcgtcggca tcgtggcgaa ccagcggaag atcgtccggg cccgcgccgg ccggaaggcg
      421 cataccgacg agatgcaggc cggcggcgtg atctactcgg actcggcgga caaggccgcc
      481 cggttcatca tggtctgcaa ccagaagcgg attcctctgg tcttcctgca ggacgtgacc
      541 ggcttcatgg tgggtacccg ggcggagcag ggcggcatca tcaaggacgg cgcgaagatg
      601 gtcaacgcgg tggccaattc cgtcgtgccg aagttcaccg tggtgaccgg caattccttc
      661 ggcgccggca attacgccat gtgcggccgc gcctacgacc cgcgcctgat gctggcctgg
      721 ccgaccgccc gcatcgccgt gatgggcggc aaccaggcgg cggagacact gctccagatc
      781 cacgtggcga agctcagccg gcagggcatg gagccggacc ggctacagca ggacgagctc
      841 ctccggagca tccgggcccg gtacgaggag accacgtcgc cgtactacgc ggccgcgcgg
      901 atgtggatcg acgagatcat cgacccgcgc ctcacgaggc gctggatctc gaccggaatc
      961 gagatggcgg atcacaatcc cgacatggag ccgttccgga ccggagcgat ccagacctga
     1021 ccggccatgt ccttccgctc gactccccgg cgcttccacg actgggtccg cgtgcggccc
     1081 gacgcgcccg cctactacga gcggtcgggc ggacgctggg tgcccacgga ctggaagacc
     1141 tacacgggcg aggtgcgcgc tgcggcgaag gcactcatgg cgctcggctt cgagcccggc
     1201 acgatggtcg cgatcatggg gttcaacgcg ccctcctgga cgatatcgga cctggcggcc
     1261 atgacggccg gggggacgcc ggtcggcgtg tacgcgaaca gctcgctcga ccaggtgggc
     1321 ttcatcctgg ggcactccgg ggcccgattc ctcgtgatcg acgggatgga cgcctggaga
     1381 cgggtccgct ccgaggtcga gcgcctgccg gacctggagc atgtcgtctt catgcgcgga
     1441 gcggaagcct cggatgaccc ccgggtgctg tcgtgggacc gcttcctggc gcacggcacg
     1501 cgggtagcgg aggcggacct ggaccgccgc atggacggtc ttcgccccga ggacgtcggg
     1561 gccctgatct atacctccgg cacgaccggt ccgccgaagg ggagcatgct cagccacgga
     1621 gccctcgcgg cggccacgga catgggcatc gaactactcc ccgatcctca catcggggca
     1681 cgcctgcttt cgtacctgcc gatggcccac gcggcggagc gcgcgctcac cctgctgggt
     1741 cccgcttcgt acggatacac cgtgtattac gcggagtcca tcgacgccat gcccgccaat
     1801 ctccgggagg tggagccgga gatcttcctg ggcgttccgc gggtctggga gaagatccgc
     1861 gccggcgtgg aggagcggat cggcgcgatc cgggggccca ggcgccggct catcgcatgg
     1921 gcccggtccg tggcgctgag cgtccacgag gagcgctgcc ggggcatgga gctccgcgga
     1981 tcgctcgcgc gggattatcg atgggccaag cgcctggtgc tggacaggat tcggaccgcc
     2041 gtaggcctgg ggaggagtcg cgtgctgctg tccggggccg cgccaatggc ggaggaggac
     2101 ctgcgcgccc tggcctcgct ggacatgccc gtccgcgagg tgtacggcca gacggaggac
     2161 tgcgggccga ccacgaacaa ccgcccgggc gagacgcgct ggggaagcgt gggccgcccc
     2221 ttccgcggct gcgaggtgaa gatcgccgac gacggcgagg tgctggttcg ggggccccac
     2281 ctgttctcgg ggtacttccg ggacccggag gcgagcgccg aggccctgcg ggacggctgg
     2341 ctccactcgg gggacctggg cttcgtggac acggacggct acctgttcat caccggtcgg
     2401 aagaaggaga tcatcatcac gtccggcggc aagaacgtgg ctccccggaa cctcgaggag
     2461 gcgatccggg aatccccgct cgtcgaggac gtcgtgctcg tcggggacgc ccggaagcat
     2521 ctcggggcgc tcgtgacgct tcgtcccggg gccgtgagga gcctgcagga ggagtcgggc
     2581 ccctccggca cggtgcctgc ccacaccgac cggatcgtgc tgaccgcgat ctgggagcac
     2641 gtggaacgcg tgaatgaccg gttctcccgg ctggagcacg tccggcgcat catggtgctc
     2701 ccgggtctct tctcggtgcg ttccggggaa ctgaccccga cgctgaaggt ccgacgggag
     2761 acgatcgccc gccactatgc gaaggagatc gacctcctgt attcgggcac gccgatcgac
     2821 ggcaccggcg tcgtgcccgc gccagacgaa cccccgcaac ccggctccag cgaaccgacc
     2881 tgaaccgcca tggccaggga caagcccatc tggtttcgac ccctcacccg caaggcgctc
     2941 aacgccatgt gcaaggacac gctgatggag gcgcttggca tcgaattcgt cgaagtcggc
     3001 cctgatttcc tggccgcacg catggcggtg gaccgtcgca cccgccagcc catgggactc
     3061 ctgcacggcg gcgcctccct cgccctcgcg gaatccgtgt gcggcgcggc ggcgtcggcg
     3121 agcgtggacg ccgatcggtt cgcctgctac gggatggaga tcaacgccaa ccacgtacgt
     3181 accgcgaagg gcggggaagt ggtctgcacg gcccggccgg cctacctggg cgcgacgacg
     3241 caggtgtggc agttcgaaat ccggaacgcc tcgggcgacc tcctgtgcac cgggcggcag
     3301 accgtcgccg ttcgccccgt cgtgctcccg gaatgacagg cggagcacga tcatccccgc
     3361 acggcgggac gcgaaccggg cgacgccccg gccgttcgcc atcggacacg ggccacttcc
     3421 cgacgcggac ccatgaacga atccgatgag gccgaggggg ccgggcgccc cgcgacgcgg
     3481 cgcagggctc cgttcgacgc ggagcgggcc ggccggctcg cgcgggacgt ctggggcctg
     3541 gaggcccgcg ccacggagct ggtcggcgac atggaccgca acttccggct ggagacggcc
     3601 gacgggacgg tcgtcgctct gaagatcagc cgggtcggcc gggaccacct gccgctgtcc
     3661 gtgcggcagg ccgccgtcga tgccgtcgcc acccggacag gggcgccgcg ggtgccccgc
     3721 agcgtgcccg cgtcggacgg tcggaccgtg gtgagcatcg agggccccgg cggggccgtg
     3781 ttcgaagccg cgtgcacgga atgggtcgag ggcacgccgc tcgtcgactt ccggccgaga
     3841 accctcgcgc tgtgccgcga cctggggcag ggcctcgcca tggtgggaag cgcgctggaa
     3901 tcgacgcgtc acgacgcgct cgaccgcgcg tccgactggg acctcaggag gggattcgcg
     3961 gtcgccgagc ggcatgcagg ggccgtggac cccgggcggc gcggcctgtt cgaccggacg
     4021 ctccgccgag ccggggaggc ggccggcgac tgggaccgtc tggcgctcgg cgtgatccac
     4081 ggcgacgcga acgaccacaa cgtgctcatc ttgctggatg gcgccgtccc ggtgcccggg
     4141 ctcatcgact tcggggacat gatccgctca tggcgggccg ccgagccggc gatcgccgcg
     4201 gcgtatgcgg cgatggatca accggatccg gtcggcgtga tcgcggcggt cgcagccggc
     4261 ttcgatgccg ggatgccgct cgacgagcac gaactggacg ccttgccgca actcgtcgcg
     4321 ctgcgcctgt gcgtcagcgt ggccctgtcg gccgtccgct ccgcgcgtga gcccgacgat
     4381 ccctacctga cggtgagcga gaggccggcc tgggatctcc tggagcggct cgacgcggtg
     4441 catccgaggc tggccgccgc gcgcatccgg gccgcgtgcg ggcgcgagcc gtctccgcgg
     4501 ggcgcggccg tctcctcgtg gctgcgatcg gacgcggccg atcccgcccc ggtgctggcc
     4561 gccgccgggc ccgtcgccgt gctggacctc tcggtcggag gggcggatgc tgcgggactg
     4621 cgcgatccgg cacggtggtc ggaaacggtc cggagaatcc tcgacgacgc gggcgcctcc
     4681 atcgggatcg ggcggtacct cgagccgagg ctctgctacg gagcagagca gttcgaggcc
     4741 ggcgagcgcg agccggagtt ccagcgcacg gtccacctcg gggtcgacct cttcgcgccg
     4801 ccaggaaccc gtgtccgggc cccgttcgac gggcgcgtgg cggccgtcgc cgacaatgcg
     4861 cacccgctgg attacgggcc gacggtcatc ctccaccacg gcctggatgg cggcgggccg
     4921 gaattctgga ccctgtacgg acacctggat ccagacgtgc tcgagcatct cgcgccgggg
     4981 cggcacgtgg ccaggggaga ggccttcgcg aggatcgggc gcgaggaccg gaacggcggg
     5041 tggccgcccc acgtccactt ccaggtcatc gccgaccggc tggaccggac ggacggcgac
     5101 ttccacgggg tggccctgcc gtccgaaacc gtggcctggg ccgcgctgtg cccggagccg
     5161 ccgatcggat tcgcggtcga cgcccggtac cgcgaggagc cggacgagcg tctcgcggcg
     5221 cgccgcaggg cccggatcgc accgagcctg gggctgtcgt acagccgccc cgtccacatg
     5281 gtgcgcgggt ccggaacctg gatgttcgac gagcgcggca ggcggtatct cgactgtgtg
     5341 aacaacgtga accacgtggg acacgcacat ccgcgggtcg tcgacgccgc gatgcgccag
     5401 atggagctgc tcaacacgaa cacccgctac ctgcacggga acctggtgcg gtacgcggag
     5461 cggctcacgg ccacgctccc ggctccgctc gacacctgca tcctcgtgaa cagcggctcg
     5521 gaggccaacg acctcgcgtt ccgcatcgcc cgctcggcga cgggccggcg cggcatcgtg
     5581 gtcgtggacg gggcctacca cgggcacctg acctccctca tcgacgcgag cccgtacaag
     5641 ttcgacggcc cgggcggatc ggggcgggcc gagcacgtcc gcgtggtccg gatgccggac
     5701 gcgtaccggg gagagatccg cggtgacgac ccggacgtcg gcccgtcgta cgccgacgac
     5761 gtgcgcgagg cggcggcttc gctcgcgacg agcggcctgc ccgcggccgc gttcatcgcg
     5821 gagtcgatcc tgagctgcgg tgggcagatc gttcccccgg agggctggct gcgctccgcc
     5881 gccgcgcatg cgcgcgctgc cggggcgctg
//
LOCUS       DBNHQZ010000206         2921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_875942, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000206 DBNHQZ010000000
VERSION     DBNHQZ010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_875942"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16YU_05445"
     CDS             <1..352
                     /locus_tag="AC16YU_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064701.1"
                     /translation="AAIRELAGMLFDAHAMEEAADLYARYLALEPAARQAWLDLANAQ
                     AALRRWEDAERTMLGMLDAFPDDPAATWNLGAILANQGRTEEAATWWHRTVELADTAL
                     AGQARRALSTLYGP"
     gene            406..2070
                     /locus_tag="AC16YU_05450"
     CDS             406..2070
                     /locus_tag="AC16YU_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064702.1"
                     /translation="MLSALAPGAAAQLISPGRLSSAHEALDGISSCTSCHALGERGIS
                     AAKCLGCHEPLAVRIAAGRGLHGRMEQANCGDCHAEHYGREFRIVRFDTARFDHADTG
                     YRLVSAHAAAACRSCHRPSFVADPVVLRFKAPAGATGRTYLGLDPRCEGCHEADDPHG
                     GQFAAPDCSTCHDEARWARAARFDHAKTDFPLTGLHRGVACVSCHHSRTSVPGPGTLR
                     YSGIAFAGCASCHRDPHEGRMGTACAGCHGTDGWSKLTGGTFATAAEHSRTGFDLEGA
                     HAAAPCSSCHERGATTSRTVRLRFASGSAGRRFPVPAHDSCADCHLDAHAGGLPMRGG
                     ASPCTDCHTTAAWAPSNFAPDRHASETRFALTGAHVAVACSGCHVTDRPALRLDFRHG
                     RTDCAGCHAKDDPHRGQFEAGPDSVSCASCHVTDEWRQVAFDHARTRFPLSGRHATAA
                     CVSCHRSGEGEPVPYRGLDTSCVSCHGEDDPHAGQFADRPCSECHDAGSFRMEAFDHT
                     RTRFPLDRAHERVPCGRCHVPESGPDGRSFVRFRPIDTRCTACHAS"
     gene            2055..>2921
                     /locus_tag="AC16YU_05455"
     CDS             2055..>2921
                     /locus_tag="AC16YU_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064703.1"
                     /translation="MPRFLTILAMWGVLALGTVGPVALDAAAQDRPAAPVDSSGVAAR
                     VTYLASAAVYLDAGRDQGIHQGDTLDVSAGGRELGRLLVTGTARARSVATFAGAPFDV
                     AVGDTLLLRVAGRPPPSPVGLPSGGAVADSTAGAGRPSILAAETGSGRADARTRRPVR
                     FSGRIRVSVSGRRSETGSGLFDSSARTIASPTVAVRLAAADLPGGWDAVLDARLSRRF
                     SSNSIVGSDGQARIYRLSLARSGFGSPLDVEAGRFPLRQDGAHAWWDGVRGGYRGQRV
                     SAGVAFGMEPDAF"
BASE COUNT          360 a         1080 c         1068 g          413 t
ORIGIN      
        1 cgcggcgatc cgcgagctcg ccgggatgct cttcgacgcg cacgccatgg aggaggccgc
       61 cgacctgtac gcccggtacc tcgcgctgga acctgccgcc cggcaggcct ggctcgacct
      121 ggccaacgcc caggcggcgc tccgccgctg ggaggacgcc gagcggacca tgctgggcat
      181 gctggatgcg ttcccggacg acccggccgc cacgtggaac ctgggcgcca tcctggccaa
      241 ccagggccgg accgaggaag cggccacatg gtggcaccgc accgtggaat tggccgacac
      301 cgcgctcgcc gggcaggccc ggcgggccct gtccacgctg tacggcccgt gatcggtccg
      361 cgtccatatc gagcgcccgg ccgtcggatc ctgctctgga ccatcgtgct ctccgccctt
      421 gctccgggcg ccgccgcgca gctcatatca cccggcaggc tgagttcggc gcacgaggcg
      481 ctcgatggca tctcaagctg caccagctgc cacgctctcg gcgagcgggg catctccgcg
      541 gccaagtgcc tcggctgcca cgagcccctc gccgtgagga ttgcggccgg acggggcctg
      601 cacggccgca tggagcaggc gaactgcgga gactgccacg ccgagcatta cggccgcgag
      661 ttccggatcg tgcggttcga tacggcccgg ttcgaccacg cagacacggg gtaccgcctg
      721 gtctcggcgc atgccgcggc agcatgccga tcctgccata ggccgtcctt cgtcgcggac
      781 ccggtcgtgc tccggttcaa ggcgcccgcc ggcgcgaccg gccgcacgta cctgggcctc
      841 gatccgcggt gcgagggctg ccacgaggcg gacgatccgc acggtggcca gttcgcggcc
      901 cctgactgct ccacgtgcca cgacgaggcc cgctgggcgc gcgcggcgcg gttcgaccac
      961 gcgaagacgg acttcccgct gacgggtctg caccgagggg tcgcctgcgt gtcctgccat
     1021 cactcgcgca cgtccgtccc cggaccgggg acgctccggt actcgggcat cgcgttcgcc
     1081 ggctgcgcgt cgtgccaccg ggacccgcac gagggacgga tgggaacggc ctgcgcgggc
     1141 tgccacggga cggacggatg gtcgaagctc acgggcggga ccttcgcgac ggcggcggag
     1201 cattccagga cgggattcga cctggagggt gcccacgcag cagcgccgtg cagctcgtgc
     1261 cacgagcgtg gggcgacgac ctcgaggacg gtccgcctgc gattcgcttc cgggagcgcg
     1321 ggacggcgtt tccccgtccc cgcccacgat tcgtgcgcgg actgccacct ggacgcccac
     1381 gcgggcgggc tgcccatgcg gggcggcgcg tcgccgtgca cggattgcca taccacggct
     1441 gcctgggcgc cctcgaactt cgctccggac cggcacgcgt cggagacccg gttcgcgctc
     1501 acgggcgccc acgtggccgt cgcctgctcg gggtgccacg tgacggaccg gcccgcgctg
     1561 cggctggatt tccgacacgg gcggaccgat tgcgcgggct gtcacgcgaa ggacgacccg
     1621 caccgcgggc agttcgaggc cgggccggac agcgtctcct gcgcatcctg tcacgtgacc
     1681 gatgaatggc ggcaggtcgc cttcgatcac gcccggaccc ggtttcccct ttcgggcagg
     1741 cacgcgacgg ccgcctgcgt ctcctgccac aggagcgggg aaggggagcc cgtgccgtac
     1801 cggggcctgg acacctcgtg cgtctcctgc cacggggaag acgatcccca cgcgggccag
     1861 ttcgccgacc ggccgtgctc ggaatgccac gatgccgggt ccttccgcat ggaggcattc
     1921 gaccacaccc gcacccggtt cccgctggac cgggcgcacg agagggtgcc gtgcggccgg
     1981 tgccacgtcc cggagtccgg cccggacgga aggtccttcg tccgcttccg ccccatcgat
     2041 acccggtgca cggcatgcca cgcttcctga cgatcctggc catgtggggc gttctcgcgc
     2101 tcggcacggt cgggccggtg gcgctggatg cggcggcaca ggaccggccg gccgcgcccg
     2161 tcgattcgtc cggcgtggcg gctcgggtca cgtacctcgc ctcggccgcc gtctacctgg
     2221 acgcgggtcg ggaccagggc atccaccagg gcgacaccct ggacgtatcg gccgggggtc
     2281 gagaactcgg acgcctgctc gtgacgggga cggcccgcgc gcgctccgtc gcgaccttcg
     2341 ccggagcacc gttcgacgtg gcggtcggtg acacgctcct tctccgggtg gccgggcggc
     2401 ctccgccatc cccggtcgga cttccttccg gcggggccgt cgcggattcc acggccggcg
     2461 cgggtcggcc gtcgatcctc gccgcggaga ccggatcggg ccgtgccgac gcccggacgc
     2521 gaaggcccgt gcgcttctcg ggacggatcc gggtgagcgt gagcggtcgt cgctcagaga
     2581 ccgggtccgg cctcttcgac tcctcggcga ggaccatcgc atccccgacc gtcgcggtcc
     2641 ggctggcggc cgcggacctg ccgggcgggt gggatgccgt gctcgacgcg cggctcagcc
     2701 ggcgcttctc ctcgaacagc atcgtcggct cggacggaca ggcgcgcatc taccgcctga
     2761 gcctggcgcg gtccgggttc gggtcgccac tcgacgtgga ggcaggccgt ttcccgctgc
     2821 gccaggacgg cgcgcatgcc tggtgggacg gcgtccgggg cgggtaccgg gggcagcggg
     2881 tgtcggccgg cgtggccttc gggatggagc ccgacgcttt c
//
LOCUS       DBNHQZ010000207        16969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3041901, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000207 DBNHQZ010000000
VERSION     DBNHQZ010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3041901"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..2564)
                     /locus_tag="AC16YU_05460"
     CDS             complement(168..2564)
                     /locus_tag="AC16YU_05460"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR6064704.1"
                     /translation="MTASRGTPIRSIASLIAAVIPVILVVGARPVLAQVWGDPVDVSE
                     EFRKLEQVFFVGSRVAEFDPGSGRGRLQWDRYRLRPALSFNKVDLGFSRAEADEFPGT
                     EYARDPVLPFAITFVSPRTVRLRFSTRDVPIEEIPSLMLAGPVPTSDAWVAEETDEAV
                     VFTGPFGRVRIQRDPWHVEIHDAHGRLVTRTMNVGEPATFSTPVPFSFVRRARDMGRS
                     TAAAFELQHDEKLFGAGESFTRLDKRGQKLNLSTRDGMGAQTGLMYKPVPFFLSSNGY
                     GMFVHTSAPVTIDFGQAFDQSNVVYSGDETLDLFVFLGGPKDILSEYTALTGRSPMPP
                     VWSFGLWMSRITYQSEAEVRDVAAKLRQHRVPADVIHLDTGWFETDWQSNYEFSTTRF
                     DDPAAMIADLRAMGFRVSLWQLPYYTDRNSVFREVVGGGFHVRNAGGRLPEFDATLDF
                     SNPGAVAWYRAKLKGLLDLGVGVIKADFGEGAPLDGLYASGRTGWYEHNLYPLRYNQV
                     VYDVTKEVTGDGIIWGRSAWAGSQRYPLHWGGDAENTNSAMAATLRAGLSMGLSGFTF
                     WSHDVGGFVQRAPRDLYSRWLPFGALSSHMRTHGAPPREPWEYDPEMVQEFRRALGVR
                     YTLMPYILAQARQASSLGHPMMRALFFDYPDDPTSWLVEDEYLFGTDLLVAPLFSEAR
                     TRDVYLPPGAWTDYQTGRVYDGGRWHPIEAGQVPVVVLVRDHAVLPHLALAQSTADLD
                     WTSVELRVFSTDGRAATGFFALPDGDVHELSVEPGAAGPSGDPFAGSVSWHVTRGGPR
                     "
     gene            2899..3882
                     /locus_tag="AC16YU_05465"
     CDS             2899..3882
                     /locus_tag="AC16YU_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6064705.1"
                     /translation="MTHAIHPAVLPRRNGTAHVPSPGAQAEDAAPLVPGIGGTPLFAL
                     SHIGAHLRPGVRLLAKGEHLNPGGSVKDRPAERMIRSGIASGRLHSGVTLIDATSGNT
                     GIAYAMIASRLGLRVTLALPENASPERKAILRAYGADLVLTDPLEGTDGAQAEVAARV
                     ARDPDRFFHPNQYDNDENWRAHFAGTGPEILAQTGGRVTHFVAVLGTTGTFTGVTRFL
                     HRHDPEIRAISVQPDSPMHGLEGMKHMATARVPGIYDPTLADGELTISTGDAETMARR
                     LATEEGLLVGPSSGANVAAALSVAETLEEGVVVTVLCDSGMRYLSHPLWNR"
     gene            3888..4343
                     /locus_tag="AC16YU_05470"
     CDS             3888..4343
                     /locus_tag="AC16YU_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR6064706.1"
                     /translation="MRATKDVIAAIREHGEATYPEECCGFFLGRLEGFDPLEPGHEVT
                     GTLPAPNRHPDGRERRFTIDPADWLAAEREAARLGLDVVGVYHSHPDHPARPSATDLA
                     EAAFPGLAYVIVPVHGGRAGEPTAWALAHDRSRFDPEPVLLMSTRVLMP"
     gene            4340..4627
                     /locus_tag="AC16YU_05475"
     CDS             4340..4627
                     /locus_tag="AC16YU_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6064707.1"
                     /translation="MIPDVTLRIPAPLRAFTRHLSAVPVRAGTVSAALDALVDLFPGL
                     RSHLFDEHGEVRSFVNLYRNDEDVRYLDHVRTELRAGDELVIVPSIAGGAA"
     gene            4632..5801
                     /gene="moeB"
                     /locus_tag="AC16YU_05480"
     CDS             4632..5801
                     /gene="moeB"
                     /locus_tag="AC16YU_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR6064708.1"
                     /translation="MIPDSVMTDDALSTQELGRYSRHLLLPEVGMEGQKKLKASSVLL
                     VGAGGLGSPLALYLAAAGVGRLGIVDFDVVDETNLQRQVLHGTSDVGRPKLDSAADRI
                     REINPHVRVIRHETMLTSENALGIVAGYDVVADGTDNFPTRYLVNDASVLTGVPNVYA
                     SIFRFDGQVSVFGTRGGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGTIQAAEVI
                     KLLLGIGEPLVERLLLLDALSMRFTTLKVRRNPDCPVCGDHPTQTGLIDYEQFCGIPV
                     DGELVAEVPSIAVTELKIRLDRGDDLALLDVRRPEESAIASIGGTLIPVDELESRLAE
                     IEADRNREIVVICRTGVRSARAVKILERHGFLGARNLTGGIRAWSEEIDPGVPVY"
     gene            6032..7567
                     /locus_tag="AC16YU_05485"
     CDS             6032..7567
                     /locus_tag="AC16YU_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR6064709.1"
                     /translation="MLHQVSRGLRVAAAALPLLVPSTTYAQTAYFDDPTDVIQVDGQT
                     TITAGSTYEAVVLFPGDSARAGFIFNEWAAFQEDKQFTAGPTIIAGYQFPSGDVLLQA
                     GVTMTPWVWHHLAFVADGSGGRLYVDGILVASGSAGAGFGSAGKPHIGAIFRDGGIIP
                     SFVGYLQSFRVSDIGRYTGERFAPPLGDFSSDANTLLLFNFDEPAGSATVTDSGPQGL
                     VGTLGSAFEGATSPKLGVLPTHTDCGIAGVGWDLWNGSRGSQVTGSSGWLNGNDAQGP
                     DMFGAAESSPEPGNAIFRDDESAGFDHWVEWEVPAAVTFRSFHLRAAHDSPGTSRSFD
                     RFRLLSYDDQAGSFELLSETEFAVPYGGGTLGNDLSLCVNVPERTASRFRAEFRQAHG
                     GAWPGPRIFELDGFTTIIPALAVAVEEAVPQPGSFLLGAPYPNPFRTSAALPFDLDRP
                     GRVRVTLHDVLGRELAVLMEGERPAGPNEAVFAATGLPAGVYLVRLETGSSSETRTVT
                     LVR"
     gene            7688..8605
                     /gene="gnd"
                     /locus_tag="AC16YU_05490"
     CDS             7688..8605
                     /gene="gnd"
                     /locus_tag="AC16YU_05490"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR6064710.1"
                     /translation="MHIGLIGLGRMGCGLGGRLRRAGLGVTGFDPSPGARQDAADGGI
                     VTVGSIEALVERIPHPRRVWCMVPAGHATEATVTRLSELLEPGDSVVEGGNSDYRESM
                     RRHELFAARGIAMLDVGTSGGILGSETGYSLMIGGSATDTGPWRPVFEALAPGPDRGW
                     AHVGGAGAGHFAKMVHNAIEYGLMEAYAEGVALLRSEGTPEVDAVAATRAWRHGSIIS
                     STLMDLTASILDENALERVLPRVEDSGLGRLAVNTAVDRGVPLPVITQALIERIRSRD
                     EQGFAARLLAAMRGRFGGHAVPGLDADAG"
     gene            8602..9924
                     /locus_tag="AC16YU_05495"
     CDS             8602..9924
                     /locus_tag="AC16YU_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIR6064711.1"
                     /translation="MTVWLLVGIAVATVALLLGLVSVLRLPAFLALLLTAIALALATG
                     TPPGEVIGLVQAGMAGTLGYIAVVIGLGAMIGAMLERSGGAAAIADAFVRRLGPERAP
                     WGLLATGLLVATPVFFDVALVLLIPFAYAAARRTGRPMLLYAIPLLAGIAVAHSFIPP
                     TPGPVAVAGLVGADLGRVVVVGILAGVPAAVVGGIVFGRRVAGRVEPDLSVIPSVGDA
                     PAEGRVGAGAALSVVLLPLVLILAGTAWAAGSGGASWVSLATHPFTALAAGLLGAIWV
                     LGHRAGIPGREVARICNRGLEPVGLIILVTGAGGVLGKVLVAAGAGDVIAGAASGSGM
                     PVLLLAFGTAVLVRVVQGSATVSMVTAAGLVAPLLVGASGWLPALTTIAIASGATVLS
                     HVNDSGFWLVGRYVGLSEGDTFRVWTVLETILGGVGFVAVLLLSLILS"
     gene            complement(9949..11070)
                     /gene="cfa"
                     /locus_tag="AC16YU_05500"
     CDS             complement(9949..11070)
                     /gene="cfa"
                     /locus_tag="AC16YU_05500"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HIR6064712.1"
                     /translation="MNAKPYRNLVEDLLGSAGVRVNGSNPWDIRVNDERFFRRVLADG
                     DLGFGESYMDGWWDADRVDELVARIMLAGLNARVGRSWSIAARIALARVTNRQSRRRA
                     FEVGERHYDLGNDLFSCMLDRRMNYSCAYWKDAVSLDEAQERKLDLLCRKLALRPGMR
                     VLDIGCGWGAFGRYAAERHDVEVVGITVSREQAELGRSLCEGLPVEFRLTDYRDLHGS
                     FDRIVSVGMFEHVGLRNYRAYFEVAARCLAEGGLFLLHTIGSNRSITSIGRWTDRYIF
                     PNGMLPSIAQIGRALEGNFVMEDWHNLGAHYDRTLMAWHENVTGNWAKLQARYDERFR
                     RMWTYYLLSSAGSFRARNLQVWQLVLSKTGVPGGYERIT"
     gene            complement(11067..11600)
                     /locus_tag="AC16YU_05505"
     CDS             complement(11067..11600)
                     /locus_tag="AC16YU_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIR6064713.1"
                     /translation="MRPPVILLMGVSGSGKSTVGRLLAERLRVRFVDGDDLHPAANVR
                     KMSAGAPLTDEDRWPWLDAVRREIAGALSRGAGLVVACSALKDAYRRRLIEPREPVRV
                     VFLTGSREIIAERMRARESHFMPEGLLESQFRDLEAPGDALELDIRLDPADLVERALS
                     DIRGRAPAVRERDGERP"
     gene            complement(11737..13524)
                     /locus_tag="AC16YU_05510"
     CDS             complement(11737..13524)
                     /locus_tag="AC16YU_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR6064714.1"
                     /translation="MALPADHRHTLRFSSALLLVSLASACARPDPPPVPESSKTLGDK
                     SAVTAGSKPMAPVKAIDQLTAGSPVYGSLTSSDRTFTDGTYFDVWPYAGTAGEQVTFT
                     LESDDFDTYLILRLMRSSGLDDAEEVGTDDDSAGDLNSTLTLTLPETGTYAVIANSVS
                     AGVQGSYLLSASSSGASTRPPVDVSGLQPIAVNEVVSGALAGGDRTIGDGVYADLYTF
                     DVPSGSLITTAVRSGEFDAMLHLGRIVDGDVVTLASDDDSAGGTDARAEWRVEEGGTY
                     VVAASSFAAGTTGSYVIAVSVTRPIDYAARYPGGGDPDGRYALLVGIDDYPGTESDLP
                     SSVDDVEVFRQMLVEQLGFAESNIVSITDQEAFRDHVIHAFERHLGQAGPNGAAVFYY
                     SGHGTQLDENLGSDVEPDGKDEALYIWGNDFRGSALVDEEIGALANRLATDRVLVVLD
                     ACHSGTGTRGAGGQYPIKEVNFADIEATTDLPLTLVSNKGTGGAGDPNNDIAGGPSRH
                     LLLSAAASNEYALASHEEWPGIGRKASVFTYYLTQAIASMGTGATWEQIMDDVRPKTA
                     NYSAAQDNAQTAQLEGVAGTTTVADFLGL"
     gene            13678..16002
                     /locus_tag="AC16YU_05515"
     CDS             13678..16002
                     /locus_tag="AC16YU_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR6064715.1"
                     /translation="MRATMLGAAMLLLAVPVTPVGAQARRALLVGISDYGAHGTDALR
                     GRGLGNLEGPVNDVEAVRSLLVARFGFRPEDILELTDAAATRDAILSGVRDHLGRSRA
                     GDHALFFYAGHGSWVENSLSDEADRRDETLVPADAGSGAPDIRDKELRGPFSEILDRG
                     ADVTLIFDACHSGSITRAMEEGRDRSVRPAPGDIADGTVYPDPPQEHRSGRALVLSAA
                     RDDQKAREFRTEDGVVAGAFTWALVDALRDAPKGVPSAYLFQQLRALMKERGFEQEPV
                     FEAGEPRRAEPMLSADPDERYSGAVVPILSIESGGSVVLQGGVALGLRPGTELVSADR
                     NGRDARARLRVARSLGVARSRADLVAGSLRGLEAGDLLAVDRWAADPSAPLRFWIPPA
                     ASDVEEAESVFRAASLLRSDPGIRWVDDPTLDPPTHTLFWTGTEWRLSGPVGATDLGP
                     APTVSGLREALAREDRPVALFAALPPGPGTREAVMQESGRSQSGPGVAIAEDPGGETY
                     RLVGTVERGERQYYWLHVRAAAGGSPGGMPPTTEPVAGDRRAVSRSLVAQVDRLARSW
                     GWLHLWSPPGGADFPYRIGGFERERDGRPVALGDTLTWGERYRVVLEAPAAALDAGVR
                     AAFDGGTARWYWYLFALDREGIGTLLYPRASNQPNQVNLLAPGAAGRTRLAVPGDAGW
                     LFSPAASNPGARSDFATLFLLASAEPLPDPAAVLNFTGVGSRAAVDEDTEVVEAGTLS
                     RLLFDLGSGDRSEARTGPVHWAIQRAPLASIAPR"
     gene            complement(16006..>16969)
                     /locus_tag="AC16YU_05520"
     CDS             complement(16006..>16969)
                     /locus_tag="AC16YU_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR6064716.1"
                     /translation="VADALRSHGYEVELLRDLPSDRMGDEIERFIKRNGQKKNSSIVF
                     YYAGHGVSVPNIAGVEQGYLVPVDTPSPAADSTAFLNALLPIYEFGNYAQRMQARHAL
                     FLFDACFAGTIFKDLGLERDDTPPPAVADLLARPARSVITSGSAGQKVPDESLFRLHL
                     VRALSGEAGSFGDGFLTGEELGSFLRATVMNEGGGQQRPQFGKLMLAGLAEGDFVFSL
                     SAIARSASAAKAFRRWLDDTRDTTPQLKKAEPSEKSLFLFMQMGFGAAEEYDADENLL
                     AEDRMNVWKVFLDAFSEDLGAVTEDDTMRETAIERYDHWRKLAK"
BASE COUNT         2433 a         5894 c         5965 g         2677 t
ORIGIN      
        1 ctgcatcacg tggcagttgg cgcacgccgc cgcatcgttc gagaagtagg atgcgccgtc
       61 ggcatacgag aacgtgaaga gcccgaggcc cagggcggcg ccgaggaaga cgaggatcag
      121 ggttcgcatc ggaaggggac agggggagac ggggcgcgga gtcggggtca gcgcgggcca
      181 ccccgcgtca cgtgccagga gacgctgccg gcgaagggat ccccggacgg tcccgcggcg
      241 ccgggctcga ccgagagctc gtgcacgtcg ccgtccggaa gcgcgaagaa cccggtcgcg
      301 gcccgtccgt ccgtcgagaa gacccgcagc tcgaccgacg tccagtccag gtcggccgtg
      361 ctctgggcga gggcaaggtg cgggagcacc gcgtggtctc gcacgagcac cacgacgggg
      421 acctgccccg cctcgatggg gtgccaccgt ccgccatcgt acacgcgccc ggtctggtag
      481 tcggtccacg cgcccggcgg caggtacacg tcgcgcgtgc gcgcttccga gaagagcggc
      541 gccacgagca ggtcggtgcc gaagaggtac tcgtcctcga cgagccagga ggtcggatcg
      601 tccgggtaat cgaagaagag ggcccgcatc atcgggtggc ccagcgagga agcctgcctg
      661 gcctgcgcca ggatgtaggg catgagcgtg tagcgcacgc ccagggcccg gcggaattcc
      721 tgcaccatct ccgggtcgta ctcccagggc tcgcgcggcg gtgcgccgtg cgtccgcatg
      781 tgggagctga gcgcgccgaa gggcagccac cggctgtaca ggtcgcgcgg cgcgcgctgg
      841 acgaagcccc ccacgtcgtg actccagaac gtgaagccgg agaggcccat cgagaggccc
      901 gcccgcaggg tcgcggccat cgccgaattc gtgttctcgg cgtccccacc ccagtgcagc
      961 gggtagcgct ggctgcccgc ccaggcgctg cggccccaga tgatgccgtc gcccgtcacc
     1021 tccttggtca cgtcgtagac cacctggttg tagcgcaggg ggtacaggtt gtgctcgtac
     1081 cagcccgtgc ggccggacgc gtacaggccg tccagaggcg cgccctcgcc gaagtccgcc
     1141 ttgatgacgc cgacgcccag gtccagaagc cccttcagct tggcccggta ccacgccacc
     1201 gcaccgggat tcgagaagtc gagagtcgcg tcgaactcgg gcaggcgccc gcccgcgttc
     1261 ctcacgtgga agccgccgcc cacgacctcc cggaagacgg aattccggtc ggtgtagtac
     1321 ggcagctgcc acaggctcac gcggaatccc atggcgcgca ggtcggcaat catggccgcg
     1381 gggtcgtcga atcgcgtggt cgagaactcg tagttcgact gccagtccgt ctcgaaccag
     1441 cccgtgtcca ggtggatcac gtcggccggg acccggtgct ggcgcagctt cgcagcgacg
     1501 tcccgcacct ccgcctccga ctggtacgtg atccggctca tccacagccc gaacgaccag
     1561 acgggcggca tgggactgcg cccggtcagc gcagtgtact cggagaggat gtccttcggc
     1621 ccacccagga acacgaagag gtcgagcgtc tcgtcgccgg agtacaccac gttggactgg
     1681 tcgaaggcct gcccgaagtc gatcgtcacg ggggcgctcg tgtgcacgaa catcccgtag
     1741 ccgttcgagc tcaggaagaa ggggacgggc ttgtacatga gtcccgtctg cgcccccatc
     1801 ccgtctcggg tggacaggtt cagcttctgc ccgcgcttgt cgagtctcgt gaacgactcg
     1861 cccgccccga agagcttctc gtcgtgctgc agctcgaagg ccgcggccgt ggagcggccc
     1921 atgtcgcgcg ctctccggac gaaggagaag gggaccgggg tcgagaaggt tgcgggctcg
     1981 cccacgttca tggtccgggt gacgaggcgg ccgtgggcgt catggatctc gacgtgccag
     2041 ggatcgcgct ggatccgcac gcgtccgaag ggccccgtga agaccaccgc ttcgtccgtc
     2101 tcctccgcca cccacgcatc ggacgtgggc acgggccccg cgagcatcag ggaggggatc
     2161 tcctcgatcg gcacgtcccg cgtcgagaat cgcagacgca ccgtgcgcgg gctcacgaac
     2221 gtgatcgcga aggggagaac ggggtcccga gcgtactcgg tgccggggaa ctcgtccgcc
     2281 tccgcccggc tgaatcccag gtccaccttg ttgaaggaga gcgcgggccg gagccggtag
     2341 cggtcccact ggagccgccc gcgtcccgat ccgggatcga attccgccac gcggctgccg
     2401 acgaagaaca cctgctcgag ttttctgaac tcctcgctga cgtccaccgg atcgccccag
     2461 acctgggcca ggaccgggcg cgcgcccacg accagaatca cgggaatgac ggccgcaata
     2521 agggatgcga tgctccggat cggtgttccg cgggaggctg tcatcggggt ctgggctgtc
     2581 tgcgccggga aggtaatgac cacggccgac gccgtcccgc cggacgcccg gtctcgaaat
     2641 ggacattggc acccggtttg ctgaggcgtg tagtggaact cctgacacaa accatcgtca
     2701 gaagacgcat gacgcacttc gatcccatcg cctgcgcctg ttgttgctgc tgtccggccg
     2761 gccggacggg cgatgggtcg cgtcttcccg aatcgggata gcgatcctgc ggacaggctc
     2821 tgaccctgta tcgatcgccc ctccggccct gccgcgaggg gcttttttgt tgccttcatc
     2881 cagaaccgtt tcccgaccat gacccacgcg atccatccgg cggtccttcc gcgcaggaac
     2941 ggcacggcgc acgtcccgtc gccgggcgcc caggccgagg acgccgcgcc gctcgtcccc
     3001 ggcatcgggg gcacgccgct cttcgcgctg tcgcacatcg gcgcgcacct tcgcccgggc
     3061 gtccgcctcc tggccaaggg ggagcacctg aaccccggcg gttcggtcaa ggaccgcccg
     3121 gccgagcgca tgatccggtc cggcatcgcg tcgggccgtc tgcacagcgg cgtcacgctc
     3181 atcgatgcca cgagcggcaa caccggaatc gcgtacgcga tgatcgcctc ccggctcggg
     3241 ctcagggtga cgctggcgct tccggagaac gcgtcccccg agcgcaaggc gatcctgcgt
     3301 gcgtacggcg cggacctcgt tctcaccgat cccctcgagg gcaccgacgg cgcgcaggcc
     3361 gaggtggccg cccgggtggc ccgcgacccc gaccgcttct tccacccgaa ccagtacgac
     3421 aacgacgaaa actggcgcgc ccacttcgcg ggtaccggcc cggagatcct ggcccagacg
     3481 ggcggccgcg tgacgcattt cgtcgcggtg ctcggcacga cgggcacgtt caccggggtg
     3541 acgcgcttcc tgcaccgtca cgacccggag atccgggcga tctccgtgca gcccgattcg
     3601 cccatgcacg ggctcgaggg catgaagcac atggcgacgg cgcgcgtccc cggcatctac
     3661 gacccgacgc tcgcggacgg cgagctgacg atctcgaccg gggacgccga gacgatggcg
     3721 cgccgcctcg cgaccgagga ggggctcctc gtgggcccgt cgtccggggc caacgtcgcc
     3781 gccgcgctct cggtcgccga gacgctcgag gagggagtcg tcgtgaccgt cctctgcgat
     3841 tcgggcatgc gctacctgag ccaccccctc tggaaccggt gaccgccatg cgtgccacga
     3901 aggatgtcat agctgcgatc cgcgaacacg gcgaggccac gtacccggag gaatgctgcg
     3961 gattcttcct ggggcgcctc gagggcttcg acccgctgga acccggtcac gaggtgacgg
     4021 gcacgctccc cgcacccaac cggcacccgg acgggcgcga gcggcgattc accatcgacc
     4081 cggccgactg gctggccgcc gaacgcgagg ccgcccgcct gggcctggat gtcgtgggcg
     4141 tctaccactc ccatcccgac catccggcgc gcccgtccgc caccgacctg gccgaggctg
     4201 cgttcccggg cctcgcctac gtgatcgtcc ccgtgcacgg cggacgggcc ggggagccga
     4261 ccgcctgggc cctggcccac gaccgctccc ggttcgaccc cgagccggtc cttctcatgt
     4321 ccacccgagt cctcatgcca tgattcccga cgtcaccctc cgcattcctg caccgctccg
     4381 ggccttcacg cggcacctgt ccgctgttcc ggtccgggcc ggcaccgtct ccgccgcgct
     4441 cgacgcgctc gtcgatctgt ttcccgggct ccgcagtcat ctcttcgacg agcacggcga
     4501 ggtgcgttcc ttcgtgaacc tgtaccgcaa tgacgaagac gtgcgatatt tggaccacgt
     4561 tcgcacggag ctccgcgcgg gcgacgagct cgtgatcgtc ccgtcgattg ccggcggggc
     4621 ggcctgaagc catgatcccc gattccgtca tgaccgacga cgctctctcg acgcaggagc
     4681 tcggccggta ctcccgacac ctgctccttc cggaggtggg catggagggg cagaagaagc
     4741 tcaaggcgtc ttccgtgctc cttgtcggcg cgggcgggct cggctctccg ctcgcgctct
     4801 acctcgcagc ggccggcgtg gggcgcctgg gcatcgtcga cttcgacgtg gtggacgaga
     4861 ccaacctgca gcgccaggtc ctgcacggta cgagcgacgt gggccggccc aagctcgatt
     4921 ccgcggcgga ccggatccgc gagatcaacc cgcacgtccg ggtgatccgc cacgagacga
     4981 tgctcacgag cgaaaacgcg ctcggcatcg tggccggcta cgacgtggtc gccgacggga
     5041 ccgacaactt cccgacgcgc tacctcgtga acgacgccag cgtgctcacc ggcgtgccca
     5101 acgtgtacgc gtcgatcttc cggttcgacg gccaggtgtc cgtcttcggg accaggggtg
     5161 gaccgtgcta ccgctgcctg tatcccgagc cgccgccgcc cgggctcgtc ccgtcgtgcg
     5221 cggagggcgg cgtgctgggc gtgctgccgg ggatcgtggg cacgatccag gccgcggagg
     5281 tcatcaagct gttgctgggc atcggcgagc cgctcgtcga acggctcctg ctcctggacg
     5341 cgctgtcgat gcgattcacc acgctgaagg tccgccggaa tccggactgc cccgtctgcg
     5401 gcgaccatcc gacccagacc gggctcatcg actacgagca gttctgcggc atcccggtgg
     5461 acggtgagct cgtggccgag gttccgtcca tcgccgtcac ggagctcaag atccgcctgg
     5521 atcggggtga cgacctcgcg ctgctcgacg tacgccggcc ggaggagtcg gccatcgcat
     5581 cgatcggcgg cacgctgata ccggtcgacg agctcgaatc ccgcctcgcg gagatcgaag
     5641 ccgatcggaa ccgcgagatc gtggtgatct gccgcaccgg cgtgcgctcg gcgcgggcgg
     5701 tgaagatcct ggaacggcac ggtttcctgg gtgcgcgcaa cctcaccggc ggcatccggg
     5761 cctggagcga ggagatcgat ccgggcgtgc cggtgtattg aggaccgtgc tcctgcgggc
     5821 cgctccgggc attgcgaatc tcggggcgca cgcgtattga ttgccggggt gcagcggcga
     5881 tcgtgagtgc cctgaccccg ccggcctcta ccccgggccg gatcccaagg ggctgcgtcg
     5941 ccccgccgtc cgtggaggaa cggtcgtatt cctccgggtc cggtgcgacg accggccatc
     6001 acccagaccc cgggcttccc ccggccaagc catgctccac caggtgtcac gcggcctccg
     6061 ggtcgcggcg gcagcgctgc cgctcctcgt tccgtcgacc acgtacgcgc agacggccta
     6121 cttcgacgat ccaaccgatg tgatccaggt cgatggccag accacgatta ccgcggggtc
     6181 gacctacgag gcggtggtcc tgtttccggg agattcggcc cgcgccgggt tcatcttcaa
     6241 cgaatgggcg gcattccagg aggacaagca gttcaccgcc ggacccacga tcatcgccgg
     6301 ctaccagttc ccaagcggcg acgtgctcct ccaggccggc gtgaccatga cgccgtgggt
     6361 ttggcaccac ctggcgttcg tggccgacgg atccggcggg cgcctgtacg tggacggcat
     6421 cctcgtcgct tcaggatcgg ccggcgccgg gttcgggagt gccgggaagc cccatatcgg
     6481 cgccatcttc cgggacggcg gcatcattcc gagcttcgtc ggctacctgc agtcgttccg
     6541 cgtgtctgac atcggacggt acacggggga gcggttcgct ccgccgctcg gcgacttctc
     6601 gtcggatgcc aatacgctgc tgctcttcaa cttcgacgag ccggccggca gcgccacggt
     6661 caccgactcg ggaccccagg gcctggtggg taccctcgga tccgcattcg aaggggctac
     6721 ctcaccgaag ctcggcgtgc tcccgacgca caccgactgt ggcatcgccg gagtcggctg
     6781 ggacctgtgg aacgggtcgc ggggcagcca ggtcaccggc tccagcgggt ggctgaacgg
     6841 taacgatgcc cagggtccgg acatgttcgg cgcggccgag tcatcgcccg agccgggcaa
     6901 cgccatcttc cgggacgacg agtccgcggg cttcgatcac tgggtcgaat gggaagtccc
     6961 ggccgccgtc acgttccgca gtttccacct gcgcgccgcc cacgattccc cgggcacgag
     7021 tcgttcgttc gaccgattcc gccttctctc gtacgacgac caggcgggct cgttcgagct
     7081 gctgtcggag accgagtttg cggtcccgta cggtgggggc accctcggga acgatctctc
     7141 gctctgcgtc aacgtgccgg agcgcacggc gagccgcttc cgggcggagt tccgccaggc
     7201 gcacggcgga gcctggcctg ggccgcggat cttcgagctc gacgggttca cgacgatcat
     7261 acccgcgctc gcagtcgctg tggaagaggc cgtgccccag cccgggtcgt tcctgctcgg
     7321 cgccccgtat cccaacccgt tccggacgag cgcggccctg ccgttcgatc tcgaccggcc
     7381 ggggcgcgtc cgcgtcacgc tgcacgacgt gctcggccgt gagctggcgg ttctcatgga
     7441 aggggagcgt ccggcgggac cgaacgaggc cgtcttcgcg gccacgggcc tccctgccgg
     7501 cgtgtacctg gtccggttgg agaccgggtc gtcttccgag acgcggaccg ttacgctggt
     7561 ccggtaaggg gggtgcagcg aagcgcggta acggccgtgc cgaggcctgt ttttcccacg
     7621 cggtgcggcg acgtccgatt gcgtacgttg cgggcgtaga gggataccca cgacgtacat
     7681 ggatgcgatg catatcgggc tgatcggcct cggccgcatg gggtgcggcc tgggtggccg
     7741 gctccgccgc gctggcctgg gcgtaaccgg cttcgacccg tcacccggcg ccaggcagga
     7801 tgccgcggac ggcggcatcg tcaccgtcgg ctcgatcgag gcgctcgtcg agcggattcc
     7861 ccatccgcgc cgcgtgtggt gcatggtgcc tgcggggcac gctacggagg ccaccgtgac
     7921 ccggctctcc gagctcctgg agccgggaga cagcgtggtc gagggaggga actccgacta
     7981 ccgggagtcc atgcgccgac atgagctctt tgccgctcgc ggcatcgcga tgctggacgt
     8041 cggaacgagc ggcggcatcc tgggcagcga aacgggctac agcctgatga tcggcgggag
     8101 cgcgacggac acgggcccgt ggcgtcccgt cttcgaggcg ctcgccccgg gtccggaccg
     8161 gggctgggca cacgtgggcg gggcgggggc gggccacttc gcgaagatgg tgcacaacgc
     8221 catcgagtac ggcctcatgg aggcgtacgc ggagggcgtc gccctgctca ggagcgaggg
     8281 cacacccgag gtcgacgccg tcgcagccac gcgggcgtgg cgacacggca gcatcatctc
     8341 gtcgaccctg atggatctca cggcttcgat tctcgatgaa aacgccctgg agcgggttct
     8401 gccccgggtc gaggactcgg gcctggggcg gctcgccgtg aacacggccg tggaccgggg
     8461 cgtgccgctc cccgtcatca cgcaggcgct gatcgaacgc atccggtcgc gggacgagca
     8521 gggattcgcg gcccggctcc tcgccgccat gcgcggccgg ttcgggggcc atgccgtacc
     8581 cggcctggac gccgacgccg gatgacggtc tggctgctcg tcgggatcgc tgtcgcgacc
     8641 gtcgcgctgc tcctgggact ggtttcggtc ctgcgccttc ccgcctttct tgctctcctg
     8701 ctgacggcga tcgcgctcgc gctcgccacc ggcaccccgc ccggcgaggt gatcggcctg
     8761 gtgcaggccg gcatggccgg gacgctgggg tacatcgccg tcgtgatcgg cctcggcgcc
     8821 atgatcgggg cgatgctgga gcgctcgggc ggcgcggccg ccatcgccga cgcgttcgtg
     8881 cggcgcctgg gacccgaacg cgccccgtgg ggcctcctcg cgacggggct cctggtcgcc
     8941 acgcccgtct tcttcgacgt cgcgttggtc ctcctcatcc cgttcgccta cgccgccgcg
     9001 cgacgtacgg ggcgtcccat gctgctctac gcgatcccgc tcctggccgg catcgcggtg
     9061 gcccactcct tcattccgcc gacacccggc cccgtggccg tggcggggct ggtcggcgcc
     9121 gacctgggcc gcgtagtggt ggtcggcatc ctggccggcg tcccggcggc cgtcgtcgga
     9181 ggcatcgtct tcggacggag ggtggcgggc cgggtcgagc ccgacctgtc cgtgatcccg
     9241 tctgtgggcg acgcgccggc cgaagggcgg gtcggagccg gagcggcgct gtcggtggtg
     9301 ctgctgccgc tcgtcctgat tctcgcgggg accgcctggg cggccggatc gggcggtgcc
     9361 tcctgggtga gtctcgcgac gcatcccttc acggcgctcg cggccggact tctcggcgcc
     9421 atctgggtgc tcgggcaccg ggccgggata ccggggcgag aggtcgcgcg gatctgcaac
     9481 cgtggactgg agccggtggg tctcatcatc ctcgtgaccg gagcgggcgg cgtgctcgga
     9541 aaggtgctcg tagcggcggg ggcgggggat gtcatcgccg gcgcggcctc ggggtccggc
     9601 atgcccgtac tgcttctcgc cttcggcacg gccgtcctgg tgcgcgtggt gcagggctcg
     9661 gcgacggtgt cgatggtcac ggccgccggc ctcgtcgcgc cgctcctggt cggggcgtcg
     9721 ggctggcttc cagccctcac caccatcgcc atcgcctcgg gcgcgaccgt tctctcgcac
     9781 gtgaacgact cgggcttctg gctggtcggc cgctacgtgg ggctctcgga gggcgacaca
     9841 ttccgggtgt ggaccgtgct cgagaccatc ctgggcgggg tgggcttcgt ggcggtcctg
     9901 ctgctgagtc tcattctctc gtgacgggcg cgcggcccga agcccacgtc aggtgatgcg
     9961 ctcatagccc ccgggcacgc cggtcttcga caggacgagc tgccagacct ggaggttgcg
    10021 tgcccggaat gaccccgcgg aggagaggag gtaataggtc cacatgcgac ggaatcgctc
    10081 gtcgtagcgc gcctggagct tcgcccagtt cccggtgacg ttctcatgcc aggccatcag
    10141 ggtcctgtcg tagtgagcgc ccaggttatg ccagtcctcc atcacgaagt tcccctccag
    10201 ggcacgaccg atctgggcga tggagggcag cattccgttg gggaagatgt accggtcggt
    10261 ccaccgcccg atggacgtga tcgagcggtt ggatccgatc gtatggagaa ggaagaggcc
    10321 accctccgcg aggcacctcg cagcgacctc gaagtaggcg cggtaattcc tgagcccgac
    10381 gtgctcgaac atgccgacgc tgacgatccg gtcgaacgag ccgtggagat cgcggtagtc
    10441 cgtcagccgg aactcgaccg gaagcccctc gcaaaggctc cgcccgagct cggcctgctc
    10501 cctggaaacc gtgatgccga ccacctctac atcgtggcgt tcggcggcat acctgccgaa
    10561 tgctccccat ccgcacccga tgtccagcac acgcatgccg ggacggaggg ccagtttgcg
    10621 gcacaggagg tcgagcttcc gctcctgggc ctcgtccagg gagacggcgt ccttccagta
    10681 cgcacagctg tagttcatcc ggcggtccag catgcaggag aagaggtcgt tccccaggtc
    10741 gtagtgccgc tcgccgacct cgaaagcgcg tcgccgtgat tgcctgttgg tcacccgggc
    10801 cagggcaatg cgcgccgcga tggaccacga tcgtcctacc ctggcgttca gcccggccag
    10861 catgatccgg gcgacgagct catccacgcg atccgcgtcc caccagccgt ccatgtagga
    10921 ctcgccgaac cccaggtccc cgtccgccag cacgcgccgg aagaagcgct cgtcgtttac
    10981 ccggatatcc cacgggttgg aaccgttgac cctcacgccg gcgctgccga ggaggtcctc
    11041 caccaggttg cggtaaggct tcgcgttcat ggtcgttctc cgtctcgttc ccgaacggcg
    11101 ggcgcccgcc cccgaatgtc ggagagcgca cgctccacga ggtcggcagg atccagccgg
    11161 atgtcgagtt cgagcgcgtc gcccggcgcc tcgaggtcac ggaactggct ttccagcagc
    11221 ccttccggca tgaaatgact ctcgcgggca cgcatgcgtt ccgcgatgat ctctctggaa
    11281 ccggtaagaa acacgacgcg gacgggctcg cggggctcga tgagccgccg ccggtatgcg
    11341 tccttgagtg cgctgcacgc cacgacgagt cccgcgcctc gcgacagcgc accagcgatc
    11401 tcccgccgaa cggcatcgag ccagggccac cggtcctcgt ccgtgagcgg ggcgccggcc
    11461 gacatcttcc ggacgttcgc cgcagggtgc aggtcgtcac cgtccacgaa gcgcacccgc
    11521 agccgctcgg caaggagccg accgacggtc gacttgccgg agcccgagac ccccatcagc
    11581 aggatgacgg gcggccgcac gggttcgccg gcggcggtca tccgggcccg ctcccgggcg
    11641 aggtcgctcg gatggcgacc tcgcccgttg aagctcgtgc ccggggatcg gtgaagcggg
    11701 atatcaccag gtccagcgag attcggcggt ctccgctcag agccccagga agtccgccac
    11761 cgtcgtggtg cctgcgactc cctcgagctg cgccgtctgc gcgttgtcct gcgccgccga
    11821 gtagttggcg gtcttcggcc ggacgtcgtc catgatctgc tcccacgtgg cgccggtgcc
    11881 catgctggcg atcgcctggg tcaggtagta cgtgaacacg ctggccttgc gcccgatgcc
    11941 cggccactcc tcgtggctcg ccagcgcgta ctcgttgctg gccgcggcgc tgagcagcag
    12001 gtgcctgctc gggcccccgg caatgtcgtt gttcggatct cccgcgccgc ccgtcccctt
    12061 gttcgagaca agggtgagcg gcaggtcggt cgtcgcctcg atgtcggcga agttgacctc
    12121 cttgatcggg tattgcccac cggctccccg cgtgccggtg ccggaatgac aggcatcgag
    12181 gacgacgagc acgcgatccg tggcgagccg gttggcgagt gctccgatct cctcgtccac
    12241 gagcgccgag ccgcggaagt cgttgcccca gatgtagagc gcctcgtcct tgccgtccgg
    12301 ctccacgtcg ctgcccaggt tctcgtcgag ctgcgttccg tgtccgctgt agtagaagac
    12361 ggccgccccg ttcggccccg cctgccccag gtgccgctcg aacgcgtgga tcacgtggtc
    12421 gcggaacgct tcctggtcgg tgatcgacac gatgttcgac tcggcgaatc ccagctgctc
    12481 gacgagcatc tgccggaaga cctccacgtc gtcgaccgaa ctcggcaggt cgctttccgt
    12541 gccgggatag tcgtcgatgc cgacgaggag ggcgtaacgg ccgtcgggat cgccgcctcc
    12601 cggataccgg gcggcgtagt cgatcgggcg cgtgacgctc acggcgatca cgtacgatcc
    12661 cgtggtgccg gccgcgaacg agctggcggc cacgacgtag gtgccgccct cctcgacgcg
    12721 ccattctgcg cgtgcgtccg tgccgccggc cgagtcgtcg tcggatgcga gcgtaacgac
    12781 gtcaccgtcg acgatcctcc cgaggtgaag catcgcgtcg aactcgcccg accggacggc
    12841 cgtcgtgatg agagaaccgg acgggacatc gaaggtgtag aggtccgcgt agacgccgtc
    12901 cccgatggtg cggtccccgc ccgcgagggc gccggatacc acctcgttga cggcgatcgg
    12961 ctggagcccc gacacgtcca ccggcggccg ggtcgaggcg cccgacgagg acgcggaaag
    13021 caggtacgat ccctgcacgc cggcagagac cgagttcgcg atgaccgcgt acgttccggt
    13081 ctcaggcagt gtcagcgtga gcgtcgagtt cagatcgccc gccgagtcgt cgtcggtgcc
    13141 cacctcctcg gcatcgtcca ggccggacga ccgcatgagt cgaaggatca ggtacgtgtc
    13201 gaagtcgtcc gattccaggg tgaatgtgac ctgttcgccc gccgtgcctg cgtacggcca
    13261 gacgtcgaaa taggtaccgt ccgtgaacgt gcgatcggac gaggtgagcg aaccgtacac
    13321 gggcgagccg gcagtgagct ggtcgatggc cttgacgggc gccatgggtt tgctgccggc
    13381 cgtaacggcc gacttgtcgc ccaatgtctt ggacgattcg ggtacgggcg gaggatccgg
    13441 ccgggcgcag gcggacgcga gcgagacgag gaggagtgcc gaggagaagc gaagggtgtg
    13501 ccgatgatcg gcgggcaggg ccatgcagtt gaccggtatg ggggaaggag ggagaggcga
    13561 cgccggagtc tccgcggccg gagacgcggg tccgcgatgg cggcaacgcg cgcccggatc
    13621 gggcgttcga cgcaaggtac gatccgcagc gaatccccgg tcaagccccg tccgtccatg
    13681 cgtgcaacga tgctcggcgc agccatgctc ctccttgccg tgccggtcac accggtcggc
    13741 gcccaggcga ggcgggcatt gctcgtcggc ataagcgact acggggccca cggaaccgac
    13801 gccttgcggg gacgcgggct gggcaacctg gagggtcccg taaacgacgt ggaggccgtg
    13861 cgctccctcc tcgtcgcccg cttcgggttc cggccggagg acatccttga gctcaccgat
    13921 gccgcggcca cgcgagacgc catcctctcg ggtgtccggg accatctggg ccggagccgc
    13981 gccggggacc atgccctctt cttctatgcc ggccacggct cgtgggtcga gaattcgctc
    14041 tcggacgagg cggaccgtcg cgacgagacg ctggtgcccg ccgatgccgg ttccggcgcg
    14101 ccggacatac gcgacaagga gcttcgggga cccttctcgg agatactcga tcgcggggcc
    14161 gacgtcacgc tgatcttcga cgcgtgtcac agcgggtcga tcacgcgcgc gatggaggag
    14221 ggccgggatc gatccgtgcg cccggcgccg ggcgatatcg cggacggtac ggtgtacccg
    14281 gacccgccgc aggagcatcg ctcgggacgt gcgctcgtgc tctccgccgc gcgggacgac
    14341 cagaaggccc gcgagttcag gacggaggac ggtgtcgtgg ccggtgcctt cacgtgggcg
    14401 ctggtggacg cgctgcgcga cgcgccgaag ggcgtcccct cggcgtacct gttccagcag
    14461 ctcagggcgc tcatgaagga gcgcggcttc gagcaggagc ccgtcttcga ggccggcgag
    14521 ccgcgccgcg ccgagccgat gctctcggcc gaccccgacg agcggtactc cggcgccgtc
    14581 gtgccgatcc tgtcgatcga gtcgggcggc tcggtggtcc ttcagggcgg ggtggcgctc
    14641 ggcctgcgtc ccggtaccga gctcgtgagc gcggaccgga acggacggga cgctcgggca
    14701 cgccttcgtg tcgcccggtc gctcggcgtg gcccgctccc gcgcagacct cgtggccggg
    14761 tcgctccggg ggctggaggc cggcgacctg ctcgctgtcg accggtgggc ggcggatccg
    14821 tcggcgccgc tgcgcttctg gatccctccg gccgcgagcg acgtcgagga ggcggaatcc
    14881 gtgttccggg cggcgtcgct cctgcggtcg gaccccggaa ttcgatgggt ggacgatccg
    14941 acgctcgatc cgcccacaca cacgcttttc tggacgggca cggagtggcg cctctccggc
    15001 ccagtgggcg ccaccgacct ggggcctgcg ccgaccgtgt cggggcttcg cgaggcgctc
    15061 gcgcgggagg atcgtccggt cgcactgttt gctgcgcttc cgcccgggcc cggcacgagg
    15121 gaggccgtca tgcaggagtc ggggcggtcg cagtcgggcc ccggcgtggc gattgcggag
    15181 gaccccggcg gggagacgta ccgtctcgtg ggcaccgtgg agcggggaga gcggcaatac
    15241 tactggctgc acgtacgggc ggcggcaggc gggagtccgg gcggcatgcc gcccacgacc
    15301 gagccggtcg cgggcgaccg gcgggccgtg tcgcgttcgc tcgtcgccca ggtcgaccgg
    15361 ctcgcgcgct cctggggatg gctgcacctc tggtcgccgc ccggcggcgc cgattttccg
    15421 taccggatcg gcgggttcga gcgcgagcgt gacgggaggc cggtcgccct cggggatacc
    15481 ctcacgtggg gagagcggta ccgggtcgtg ctcgaggccc cggccgcggc gctggacgca
    15541 ggcgtgcgag ccgccttcga cggcggcacg gcgcgatggt actggtacct gttcgcgctg
    15601 gatcgggaag ggattggaac gctgctctat ccgcgcgcct cgaaccagcc gaaccaggtg
    15661 aacctcctgg cccccggcgc tgccgggcgg acgcgactcg ccgttccggg cgacgcgggc
    15721 tggctgttca gtcccgcggc gtcgaatccc ggtgcgcgat cggatttcgc gacgctgttc
    15781 ctgctcgcct cggccgagcc gcttcccgat ccggcggccg tcctgaattt caccggggtg
    15841 ggaagccggg ccgcggtcga cgaggacacc gaggtagtcg aggcggggac cctgtcccgg
    15901 ctcctcttcg atctcggaag cggtgaccgc tcggaggcgc gcaccggccc ggtgcactgg
    15961 gccatccagc gcgccccgct agcgtcgatc gcgccgcgct gacgcctact tggcgagttt
    16021 gcgccagtga tcgtagcgct cgatggcggt ctcgcgcatc gtgtcgtcct cggtcacggc
    16081 gccaagatcc tccgaaaacg cgtccaggaa gaccttccac acgttcatcc tgtcctcggc
    16141 cagcaggttc tcgtccgcgt cgtattcctc cgccgccccg aagcccatct gcatgaacag
    16201 gaacagcgac ttctccgacg gctccgcctt cttcaactgg ggggtcgtgt cgcgcgtgtc
    16261 gtcgagccac cggcggaacg ccttcgcggc ggaggcggat cgggcgatcg cggaaagaga
    16321 gaagacgaaa tcaccctcgg cgagtccggc gagcatcagc ttgccgaact ggggccgctg
    16381 ctgtccgccc ccctcgttca tcacggtggc ccggaggaag ctcccgagct cctcgccggt
    16441 caggaacccg tcgccgaacg atcccgcctc gccgctgagg gctcggacca ggtgcaggcg
    16501 aaagaggctc tcgtccggga ccttctggcc ggcggaaccg gacgtgatca cggagcgggc
    16561 cgggcgggcc aagaggtcgg ccaccgccgg cggcggagtg tcgtccctct cgaggccgag
    16621 atccttgaat atggtgcccg cgaagcacgc gtcgaacagg aagagcgcat gtcgcgcctg
    16681 catccgctgt gcgtagttcc cgaactcgta gatcggcagg agcgcgttca ggaacgcggt
    16741 agagtcggcg gcgggcgacg gcgtgtcgac cggcaccagg tagccctgct cgactccggc
    16801 gatgttgggc acggagactc cgtgcccggc atagtagaag acgatcgaac tgttcttctt
    16861 ctgcccgttc cgcttgatga accgctcgat ctcgtccccc atgcggtcgg acggaaggtc
    16921 gcggagcagt tcgacctcgt agccgtgcga gcgaagggcg tccgcgacc
//
LOCUS       DBNHQZ010000208        11028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3178090, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000208 DBNHQZ010000000
VERSION     DBNHQZ010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3178090"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="AC16YU_05525"
     CDS             complement(<1..1914)
                     /locus_tag="AC16YU_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6064717.1"
                     /translation="MFAHLHCRSWFSFLAGGSSPEALAAAAAEQGVGAVALTDVHGVY
                     GVVRFQKACREHGVKPLFGAEVTVDEHRLVLIAAGEDGFSNLNRLLTAAHQRSREAPR
                     ATFTELVDHAAGLLCLTGTTEGRLWQLVDAGRGEAAMRWLHQLHSVFGERLSIELASH
                     VLPGDRLRTDRLLRLAERCYVPVVATGNVRYAQPEDYRRYDLVTCIRLGITVFTDHPE
                     RPRNAEAYLKGEEALRRLIPCPAAFDRAGEIASACSLDLIPGFITPPAAAVPPPHTAR
                     SWLHERCWAGFARRYGTFAPGSGPDRVGGRAGDAGHARDAGPAIGYDARGRRVTHRPS
                     RRPARTPDPAGAVVVVRPDAPRSRDELRRRAAEQLARELSVIAQLDLDEFFLVVHEIV
                     EEARRRGIRCAGRGSAANSIVAYLLGITGVDPIEHRLLFERFLHAGRRGTPDIDVDFD
                     SERRDEIIAWMENRFGIEQTAMTATLITYRSRLALRDTAKALGWPPEVVDRMSKAVPP
                     HWGTKDLSEYRVRLERIVGASPLLQVLLDRATDLQGAPRHLGLHSGGMILSRKPLWHF
                     SPVQVSANGVSVVQFDKDDVEALGLVKFDVLGLRMLACLSEAVEIAARHDCFVPGAPG
                     VDRGGGFDVDELPL"
     gene            complement(2033..2497)
                     /locus_tag="AC16YU_05530"
     CDS             complement(2033..2497)
                     /locus_tag="AC16YU_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR6064718.1"
                     /translation="MRKSDRPLGRFVLDTNLWVDAFRNLGSEHRLHAFRHAHAPGEYL
                     SSVVASEILAGLRSAGERRRFHRSVLSVFDRTRRVVTPSDAAWRRAGETIAALREKDG
                     SVPVTRAFGNDVLIALSCREAGMTLVTDDRRDFERIARVAPFDYVVGLPGFG"
     gene            complement(2484..2702)
                     /locus_tag="AC16YU_05535"
     CDS             complement(2484..2702)
                     /locus_tag="AC16YU_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064719.1"
                     /translation="MTRSSRPEKTKKELKSFRLDPDRIRAAQRALGTATATETIETAL
                     DMVVFRQELLDGLEAMRGVDIISPDEEV"
     gene            complement(2827..3660)
                     /locus_tag="AC16YU_05540"
     CDS             complement(2827..3660)
                     /locus_tag="AC16YU_05540"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR6064720.1"
                     /translation="MSPRRAVAFLLVLAAPGALAQDQVLLFDDFSGGELDRSMWNVVG
                     PAFWVNNEQQAYVDSDSTIYLAAVEGAPNGALVLHPRFAPGFTTPRGRVCDFVSGRID
                     SRGKFEFTHGRAEARIRMPDAVGVWPAWWLLGGGRWPDSGEIDIMEYVGEKDWTGVAL
                     HGPGYSGETPLVNRYYFEDPAGVTDWHVYAVEWSAGEIVFLVDDRVAYRATRPMVEHY
                     GEWRFDSPKFLILNFALGGAYPFKTNGIGEPYNGLPAETVEAIRRGEVFMEVDWVRVT
                     R"
     gene            3936..4910
                     /locus_tag="AC16YU_05545"
                     /pseudo
     CDS             3936..4910
                     /locus_tag="AC16YU_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0031176 - endo-1,4-beta-xylanase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
     gene            4941..5237
                     /locus_tag="AC16YU_05550"
     CDS             4941..5237
                     /locus_tag="AC16YU_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064721.1"
                     /translation="MPSGFRLLGNYPNPFNPRTTIEYELERAGPVRLEVFDVTGRPVA
                     TLVDGFQPASAHRVDWNGTWSDGRAVPSGVYLYRLTLGAGGAFSTATRPMVVIK"
     gene            5278..6972
                     /locus_tag="AC16YU_05555"
     CDS             5278..6972
                     /locus_tag="AC16YU_05555"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6064722.1"
                     /translation="MHAHRLLPIGLAIAAALTAPGATAQRLDPGPQVLTFYSVVDDTE
                     QPYAIYLPKDFDESRAYPLVVSLHGAGSNHRLNLKRVFGRTNTDGENDVEASRYFPEW
                     RDVDYIVASPLARGTMGYQGVAEKDVLDMLADVERRFSIDPNRRYLTGLSMGGGGTFW
                     LAVTRPDLWAAIVPVCPAPPVETEALAMNLLNVPVYVHQGGADPVVRPEATRAWVNRL
                     RDVGVDVTYEEYPGVGHDSWAPAYADGRLFDWLDGRVRDAFPERVRFNATRYAYAKAY
                     WVRIDGLTPGAVARVDARFTGRNTLAIRTHAVDGLTLLLGGHPSVAPGEPLAVEIDGQ
                     RLELPAAESLGLHRAGDTWAPGALELPATAKRAGSEGPMSAVVADRHVYVYGTADDPA
                     PEEMAARRMQAEKAAEWSVYRGWFLGRIAVYPRVVSDRGVRQSDRDEANLVLFGTAET
                     NAVIADLSERLPLHMTGGADTHGLAYVYPANGRYVLVASGLPWWQPDPGAQGPGNVFT
                     AGIPALRLGEFGDYLLFEGHAGNVVASGRFDREWKLTPAAAEALAASNIVRVNAVQ"
     gene            6998..7804
                     /locus_tag="AC16YU_05560"
     CDS             6998..7804
                     /locus_tag="AC16YU_05560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6064723.1"
                     /translation="MKLALITGASSGIGAATAREMARLGYRVILVARSREKLEEVAAS
                     IGDAAAVEACDASDGDAVLALANRVRSRHGVPDVIVNSAGAGAWKRIEETTPAEAREM
                     MAAPYFSAFNVTHAFMRDMLARKSGTIVHVGSPASYVAWPSSVGYAAGRGALRGLHEA
                     LSADLAGTGVKSCHVVFGRVDSPYFATNVNVEDRMPGLARIVPRVKPDDCARVIARVV
                     RRPRDTTMYPAPLRITYGLFKLFPGVTRRLLRRTGYTPPDASPADRRPED"
     gene            7950..9488
                     /locus_tag="AC16YU_05565"
     CDS             7950..9488
                     /locus_tag="AC16YU_05565"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6064724.1"
                     /translation="MTRSHDLDRLQRRHAPLDMESAEFRDLGHRLVDRIADFMDSLPG
                     RPVTGGRTPSEIRRIVGDAPLPEHGAEAGALLEEAATTLFDHSLFNGHPRFWGYITSS
                     AAPIGALGDLLAASVNPNVGAFDLAPVATEIEAQTIRWIAGLIGYPTDCGGVLVSGGN
                     MANFVGFLAGRTASAGWDIRSDGLPSDGRRMRAYAGAGTHTWIQKAADLFGLGTGAIR
                     WIPTGPEGRMDTTALEERISEDRRAGDLPFLVVGTAGTVGTGAVDPLSRIAEICRRED
                     LWFHVDGAYGAMAAVLPEAPAELRNLALADSLALDPHKWLYSPIEAGCTLVRDPRHLV
                     GAFSFHPDYYLFDAHGEEQGINFHEFSMQKSRGFRALKVWLALRQVGRSGYERMIGDD
                     VALARALHEFAEAHDEIEAGTLNLSIATFRFVPHDLAGRAGQESEYLNRLNREILVRL
                     QAGGEAFVSNAVLDGVFVLRACIVNFRTTLGDVQMLPGLVARLGREVDAELRRTAPVT
                     GATP"
     gene            9537..10070
                     /locus_tag="AC16YU_05570"
     CDS             9537..10070
                     /locus_tag="AC16YU_05570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6064725.1"
                     /translation="MALRAPETIDVPGEGTLRVRFHTSMGTIGAVLFEEEAPRTVANF
                     VGLATGTSTWADPATREEVQHPFYDGLVFHRVMPEFMIQFGCPLGRGTGGPGYTFRDE
                     FGPGLRHDRPGLLSMANAGPNTNGSQFFITEVPTPWLDGKHAIFGEVFEGLDLVADIA
                     RAGNGAVRMERVEVYRD"
     gene            10284..>11028
                     /locus_tag="AC16YU_05575"
     CDS             10284..>11028
                     /locus_tag="AC16YU_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6064726.1"
                     /translation="MKHPALLLVLALLVVPSPVRAQDAAPTDETLSGITIRNIGPALM
                     SGRIADIAVHPADFGTWYVAVGSGGVWKTVNAGTTWEPLFDGQPSYSIGCVTLDPSNP
                     EIVWVGTGENVGGRHVGFGDGVYRSMDGGAHWESMGLAASEHVSKILVHPEDSNTIWV
                     ASQGPLWSAGGDRGVFRSTDGGATWTRVLGDDEWVGATDLVMDPRDPDRLYAATWQRQ
                     RTVASYMGGGPGTAIYRSEDGGETWQKLAN"
BASE COUNT         1675 a         3971 c         3823 g         1559 t
ORIGIN      
        1 gagcgggagc tcgtcgacgt cgaacccgcc cccgcggtcc acgcccggcg cgccgggcac
       61 gaagcagtcg tggcgcgcgg cgatctcgac cgcctcggac aggcaggcga gcatccggag
      121 gcccagcacg tcgaacttga cgagcccgag cgcttcgacg tcgtccttgt cgaactggac
      181 cacgctcacg ccgttggccg acacctgcac cgggctgaag tgccagagcg gtttccgcga
      241 gaggatcatc ccgcccgagt gcagccccag gtggcgcggg gcaccctgca ggtcggtggc
      301 ccggtcgagc agcacctgga ggagcggcga cgccccgacg atccgctcga gccgcacccg
      361 gtactccgag aggtccttcg tgccccagtg cggcggcacc gccttggaca tgcggtccac
      421 gacctcgggc ggccagccga gcgccttcgc cgtgtcgcgc agcgcaaggc gcgagcggta
      481 cgtgatgagc gtcgccgtca tcgcggtctg ctcgatcccg aaccggttct ccatccaggc
      541 gatgatctcg tcgcgccgct ccgagtcgaa atccacgtcg atgtcgggcg tgccgcgccg
      601 ccccgcgtgc aggaagcgct cgaagagcag ccggtgctcg atcggatcca cgcccgtgat
      661 cccgagcagg taggccacga tcgagttcgc ggccgatccg cggcccgcgc agcggatgcc
      721 ccggcgccgg gcctcctcca cgatctcgtg gacgaccagg aagaactcgt ccaggtcgag
      781 ctgagcgatg accgagagct ccctcgcgag ctgctccgcc gcgcgccggc gcagctcgtc
      841 gcgggagcgc ggcgcgtcgg gccgcacgac gaccacggcg ccggccgggt ccggtgtcct
      901 ggccggccgc cgggagggcc ggtgcgtcac ccgccgcccg cgcgcgtcgt acccgatcgc
      961 gggcccggcg tcccgggcat ggcctgcgtc cccggcccgg ccgcccaccc ggtccggacc
     1021 gctgcccggg gcgaacgtgc cgtagcggcg ggcgaacccc gcccagcacc gctcgtgcag
     1081 ccaggaccgc gccgtgtgcg gcggcggcac ggccgcggcg ggcggggtga tgaagcccgg
     1141 gatcaggtcc agcgagcacg cggacgcgat ctcgcccgcg cggtcgaagg ccgccgggca
     1201 cgggatgagc ctccggaggg cctcctcccc tttcaggtac gcctccgcgt tgcgcggccg
     1261 ctcgggatga tcggtgaaga ccgtgatccc gagccgtatg caggtcacca ggtcgtagcg
     1321 ccggtagtct tctggctgtg cgtacctgac attacctgta gctaccactg gaacgtagca
     1381 ccgctcggcc aggcgcagca gccggtcggt ccggaggcgg tcgccgggca gcacgtggct
     1441 cgcgagctcg atcgagagcc gctccccgaa caccgagtgc agctggtgca gccagcgcat
     1501 ggccgcctcg ccgcggcccg cgtcgaccag ctgccagagc cgtccctcgg tggtgcccgt
     1561 caggcagagg aggcccgcgg cgtggtcgac cagctcggtg aaggtcgccc gtggcgcctc
     1621 gcggctgcgc tggtgcgccg ccgtcagcag ccggttcagg ttcgagaacc cgtcctcgcc
     1681 ggccgcgatg agcacgagcc ggtgctcgtc gaccgtgacc tcggccccga aaagcggctt
     1741 gaccccgtgc tcccggcacg ccttctggaa gcgcaccacc ccgtagaccc cgtgcacgtc
     1801 ggtgagcgcg accgcgccca cgccctgctc cgcggccgcc gccgcgagcg cctcgggaga
     1861 ggaaccgccg gccaggaagg agaaccagga tcgacagtgc aggtgggcga acatgggacg
     1921 ggcacagaca ccattgccct gcccggcgtc tccggcagga catgtgcaaa gtatatgcga
     1981 cggaggccgc cggggtcaat atgtgcgttt cgcggggact cccatttcgc cgtcacccga
     2041 agccgggtag tcccacgaca tagtcgaacg gcgcgacgcg ggcgatgcgc tcgaagtcgc
     2101 gccggtcgtc ggtgacgagc gtcatgcccg cctcgcggca cgagagggcg atcagcacgt
     2161 cgttgccgaa ggcccgggtg acgggcaccg agccgtcctt ctccctgagg gccgcgatgg
     2221 tctcccccgc acgccgccag gcggcgtcgg aaggcgtcac cactctgcgc gtgcgatcga
     2281 agacggacag gacggaacgg tgaaatcgcc tgcgctcgcc cgccgagcgc aggcccgcca
     2341 gaatctcgga cgccacgacg gacgacaggt actcgccggg cgcatgcgcg tgcctgaagg
     2401 catgcagtcg atgctccgac ccgagattcc ggaaggcatc gacccagagg ttcgtgtcga
     2461 ggacgaaccg cccgagaggt cggtcagact tcctcatcgg gagagatgat gtcgacgccg
     2521 cgcatggcct cgagtccgtc gagcagctcc tgccggaata ccaccatgtc gagcgccgtc
     2581 tcgatggtct ccgtcgcggt cgcagtgccc agcgcgcgct gggctgcccg tatccggtcg
     2641 gggtccaggc ggaaggactt cagctccttc ttcgtcttct ccgggcggga tgagcgcgtc
     2701 acgaaatcgg tcattgtata tacagatgga tcttaacgta cgaccattct cgaagttgcc
     2761 ggatcccttc gcctctgaca cgcgctggcc cggatcgcaa ctccacgacg agcaacgtcc
     2821 ggccgcctac cgcgtcaccc gcacccagtc cacctccatg aacacctccc ccctccggat
     2881 cgcctcgacc gtttcggcgg gcagcccgtt gtacggctcc ccgatcccgt tcgtcttgaa
     2941 cgggtaggcg ccgccgagcg cgaagttcag gatcaggaat ttcgggctgt cgaaccgcca
     3001 ctcgccgtaa tgctcgacca tcgggcgcgt agcgcggtag gccacgcggt cgtcgaccag
     3061 gaagacgatt tcgccggccg accactcgac cgcgtacacg tgccagtcgg tcacgcctgc
     3121 cgggtcctcg aagtagtacc tgtttacgag cggcgtctcg cccgagtacc cggggccgtg
     3181 gagcgcgacg cccgtccagt ccttctcgcc cacgtactcc atgatgtcga tctcgcccga
     3241 gtcgggccag cggccgcctc ccagtagcca ccaggcgggc cagaccccga ccgcgtccgg
     3301 catccggatc cgggcctccg cgcgcccgtg cgtgaactcg aacttcccgc gcgagtcgat
     3361 ccggccggaa acgaaatcgc acaccctgcc gcggggcgtc gtgaagccgg gcgcgaaacg
     3421 ggggtgcagc acaagggcgc cgttcggagc gccctcgacg gcggccaggt agatcgtcga
     3481 gtccgagtcg acgtaggcct gctgctcgtt gttgacccag aaggccggac cgacgacgtt
     3541 ccacatcgac cggtcgagct cgccgcccga gaagtcgtcg aacaggagga cctggtcctg
     3601 ggccagcgcg cccggcgcgg caaggaccag gaggaaggcg acggctcgtc gagggctcat
     3661 ggggatcccg ggaggtttgc acggccagaa tccggcacga ccggccgttg cgcaagcccg
     3721 gagcgatggc cattccgccg tccggcccga ttcctgtcga cggggatgcc atgccgcacg
     3781 gcgaggacac cgcccggcgc ccgcgcgacc tcttcccgcg acgcggcctc cgacgaagcc
     3841 ggtgtgcgaa aacgccgcgc agccgggtcc ccgaacgccg tgcaggccgt atccttgctc
     3901 cgaccgcgta gccaacaccg agccctggac gccccatgcc tcgtttcccg cagaccctgc
     3961 gcctctccct tccggcgctc ctgctcctgc tcacccccgc cccggcccgg gcccagctcg
     4021 ccgacggcct gcccaagttc ctgggcaacg tctactcgac gagccaggcc gtcgacttca
     4081 cctcgtactg gaacaaggtg acgcccgaga acgccggcaa gtggggcagc gccgagccca
     4141 accgcgacgg ccacaactgg ggggagctcg acgcggccta cgcgctggcg aaggacaacg
     4201 gctaccccta tcaccaccac gtgctcatat ggggcaacca gcagccggcg tggatcgaat
     4261 cgctgccggt gaacgagcag ctcgaggaga tcgaggaatg gttcgcggcc gtcgccgcgc
     4321 gctacccgga catcgacatc gtcgaggtgg tgaacgagcc cctgcacgac ccgccggacg
     4381 gccccggcga cggcaactac atcgccgcgc tgggcggtac ggccggccgg gcctggatca
     4441 tgaacgcgtt ccgcatggcg cggacccatt tcccggatgc ccggctcatg atcaacgact
     4501 acgggatcgt gaacaacgcg acgagcgcgc agaattacct ggcgatcatc gagcagctgc
     4561 agacggagga cctcgtggac atcatcggcg tccaggccca cgccttctcg acccgcggga
     4621 cgcccccggc cacgatcaag gccaacctgg acctgctcgc cacttcggga ctcccgatcc
     4681 acgtgaccga gctcgacatc gactcgcaga aggaggacgg ctccctctcg gagaaggtgc
     4741 agttcgatga gtacaagcgc atcttcccga ccctctggga gcatccggcc gtcgagggca
     4801 tcacgctctg gggatggcgc atcggcatgt ggcggaccga ccagggagcc tatctcatca
     4861 attcgaacgg ctcggagcgc ttcgccctcg gctggctgcg cacctacgtc ccgatcctgc
     4921 tcgacgcgga ggaagcctcc gtgccgtccg gcttccgcct gctcggcaac tacccgaacc
     4981 cgttcaaccc gcggaccacg atcgaatacg agctcgagcg ggccggcccg gtccggctcg
     5041 aggtcttcga cgtgacgggc cgtcccgtcg ccaccctcgt ggacggcttc cagccggctt
     5101 cggcgcaccg cgtggactgg aacggcacct ggtccgatgg gcgtgcggtg ccgagcggcg
     5161 tgtacctgta ccgcctgacg ctcggtgccg ggggcgcttt ctcgacggcg acccggccca
     5221 tggtggtcat caagtagcgc gcccggggcg catcccgaaa cccaccccgc cgatcccatg
     5281 cacgcacatc gcctcctccc gatcggcctc gcgatcgcgg ccgccctcac ggcgccaggc
     5341 gccacggcac agcggctgga cccgggcccg caggtcctga ccttctactc ggtcgtcgac
     5401 gacaccgagc agccgtacgc catctacctg ccgaaggact tcgacgaatc gcgcgcctac
     5461 ccgctcgtcg tcagcctgca cggtgccgga tcgaaccacc gcctgaacct gaagcgggtg
     5521 ttcggcagga cgaacacgga cggcgagaac gacgtcgagg ccagccggta cttccccgag
     5581 tggcgggacg tggactacat cgtcgcctct ccgctcgcgc gcggcaccat gggctaccag
     5641 ggcgtggccg agaaggacgt gctggacatg ctggccgacg tcgagcgccg cttctcgatc
     5701 gacccgaacc ggcggtacct gaccgggctg tcgatgggcg gcggcgggac cttctggctc
     5761 gccgtgacgc ggcccgacct ctgggccgcg atcgtgcccg tctgcccggc gcctcccgtc
     5821 gagacggagg cgttggcgat gaacctcctg aacgtgcccg tctacgtgca ccaggggggt
     5881 gccgacccgg tcgtgcgccc cgaggccacg cgggcctggg tgaaccggct gcgcgacgtg
     5941 ggggtggacg tgacgtacga ggaatacccg ggcgtgggcc acgactcgtg ggccccggcg
     6001 tacgccgacg gccggttgtt cgactggctc gacgggcgcg tccgcgacgc cttcccggag
     6061 cgcgtgcgct tcaacgcgac gcggtacgcc tatgcgaaag cctactgggt ccggatcgac
     6121 ggactgacgc ccggcgccgt cgcccgcgta gatgcgcgct tcacgggacg gaatacgctc
     6181 gcgatccgga cgcacgccgt cgacggcctc acgctgctcc tcgggggcca tccgtcggtc
     6241 gcgcccggcg agcccctcgc ggtcgagatc gacgggcagc ggctggagct gcctgccgcg
     6301 gagtccctgg gcctgcaccg cgccggggac acctgggccc cgggcgcact ggagctgccc
     6361 gcgaccgcca agcgggccgg gtccgaggga ccgatgtcgg ccgtcgtcgc cgatcggcac
     6421 gtctacgtgt acggaacggc ggacgacccg gcaccggagg aaatggccgc ccggcggatg
     6481 caggcagaga aggcggccga atggtccgtc taccgagggt ggttcctggg ccggatcgcc
     6541 gtatacccgc gcgtggtctc ggaccgcggg gtccgacaga gcgaccggga cgaggcgaac
     6601 ctggtgcttt tcgggacggc cgaaacgaac gccgtgatcg cggacctgtc ggagcggctt
     6661 ccgctccaca tgacgggcgg cgcggacacg cacggcctgg catacgtgta cccggcgaac
     6721 ggccggtacg tcctcgtcgc ctcggggctg ccatggtggc agccggatcc gggagcccag
     6781 ggtccgggaa acgtattcac ggcaggaatc cccgcgctca ggctcggcga gttcggggac
     6841 tacctcctct tcgaaggcca cgccggcaac gtcgtggcgt ccggccgctt cgaccgggaa
     6901 tggaaactga caccggccgc cgcggaagcg ctcgccgcct cgaacatcgt gcgggtgaac
     6961 gccgttcaat gatcctggac gcaccgcgtg gcccgacatg aagctggcct tgatcacggg
     7021 agcctccagt ggcatcggtg ccgccacggc gcgggaaatg gcccgcctgg gttaccgcgt
     7081 gatcctggtg gcacggtcgc gggagaagct cgaggaagtc gcggcgtcga tcggtgacgc
     7141 ggcggcggtc gaggcatgcg atgcatccga cggcgacgcc gtcctggcgc tcgccaaccg
     7201 ggtccggtcc aggcacggcg tgcccgacgt catcgtgaac tctgcggggg ccggcgcctg
     7261 gaagcgcatc gaggagacca cgccggcgga ggcgcgcgag atgatggccg ccccgtactt
     7321 ctcggctttc aacgtgacgc acgcgttcat gcgggacatg ctcgcccgga agagcggcac
     7381 catcgtgcac gtcggatcgc ccgcgtccta cgtcgcctgg ccttcgtcgg tgggctacgc
     7441 cgcgggtcgc ggcgcgctgc gcgggttgca cgaggcgcta tccgccgacc tggcggggac
     7501 gggggtgaag agctgccacg tggtgttcgg ccgggtcgac agcccgtact tcgccacgaa
     7561 cgtcaacgtg gaggaccgca tgccggggct cgcgcgcatc gttccgcgcg tcaagccgga
     7621 cgactgcgcc cgcgtgatcg cccgcgtggt ccgccggccg cgcgatacga cgatgtatcc
     7681 cgcgccactg cgcatcacgt acgggctctt caagctgttt ccgggcgtca cgcgccggct
     7741 gctccggagg acgggataca cgccgcccga cgcctccccc gcggatcggc ggccggaaga
     7801 ctgacccctg cgatccggac ggtcacccgg tccgtgccga atcctttggg ccggcggtcc
     7861 gtagggcgtt tccggacgac ccggggtggc tctcgcctga cccccgggcg ttctcccggc
     7921 tgccccgaat ccaaccgacc cgtcgacgca tgacccgttc gcacgacctg gaccgccttc
     7981 agcggcgcca cgcgcctctc gacatggaga gcgcagagtt ccgggacctc gggcaccggc
     8041 tcgtggaccg gattgccgac ttcatggact cgctccccgg gcgcccggtc acaggtggcc
     8101 ggacgccgtc cgagatccgg cgcatcgtgg gcgacgcgcc gctccccgag cacggagccg
     8161 aggccggggc gctcctcgaa gaggcggcga cgacgctttt cgaccactcg ctcttcaacg
     8221 ggcatccccg cttctggggc tacatcacgt cgtcggcagc gccgatcggg gccctcggcg
     8281 acctgctcgc cgcgtcggtg aaccccaacg tgggagcctt cgacctggcg ccggtcgcga
     8341 ccgagatcga ggcacagacg atccgctgga tcgccgggct catcggctat ccgacggact
     8401 gcggcggcgt cctggtgagc gggggcaaca tggccaactt cgtcggcttc ctggcgggcc
     8461 gcacggccag cgccgggtgg gacatccgca gcgacggcct cccgtccgac gggcgccgca
     8521 tgcgggccta cgccggggcc ggcacgcaca cgtggatcca gaaggccgcc gacctcttcg
     8581 gcctgggcac cggggcgatc cggtggattc cgaccggtcc cgagggtcgg atggacacga
     8641 cggcgctcga ggagcggatc tcagaggacc gccgcgcggg cgacctgccc ttcctggtcg
     8701 tggggacggc cgggacggtc ggcacgggcg cggtggaccc gctttcgcgc attgcggaga
     8761 tctgccgccg cgaggatctc tggttccacg tggacggcgc gtatggcgcc atggccgccg
     8821 tgctgcccga agcgccggcc gagctccgga acctcgcgct cgccgattcc ctcgcgctcg
     8881 acccgcacaa gtggctctac agcccgatcg aggcggggtg tacgctggtc cgcgatccca
     8941 ggcacctcgt cggagccttc agcttccacc ccgactatta cctgttcgac gcgcacggcg
     9001 aggagcaggg catcaacttc cacgaattca gcatgcagaa atcgcgcggc ttccgcgcgc
     9061 tcaaggtgtg gctcgccctg cgccaggtgg gccggtccgg gtacgagcgc atgatcggcg
     9121 acgacgtcgc cctggcgcgt gcgctccacg aattcgccga ggcgcacgac gagatcgagg
     9181 ccgggaccct gaacctgagc attgcgacgt tccgcttcgt gccgcacgac ctggccggcc
     9241 gcgcgggtca ggagagcgag tacctgaatc ggctcaaccg cgagattctc gtgcgcctgc
     9301 aggccggcgg cgaggctttc gtgtcgaatg cggtcctgga cggcgtcttc gtgctgcgag
     9361 cgtgcatcgt gaacttccgc acgacgctgg gcgacgtgca aatgcttccc ggtctcgtgg
     9421 cccgcctcgg gcgcgaggtg gacgcggaac tccgccggac cgccccggtt acgggcgcga
     9481 ccccgtgacc gcgcggggaa ccacgaagtt cgcacggaaa acacggattc aaccgcatgg
     9541 cactcagggc ccctgaaacc atcgacgtac ccggcgaggg cacgctccgc gtacgctttc
     9601 acacgagcat gggaacgatc ggcgccgtcc tcttcgagga ggaggcgccg cggacggtcg
     9661 cgaacttcgt cggcctcgcc accggcacca gcacctgggc ggatcccgcg acccgagagg
     9721 aggtgcagca cccgttctac gacgggctcg tgttccaccg cgtgatgccc gagttcatga
     9781 tccagttcgg gtgtccgctg gggcgcggga ccggggggcc gggctacacc ttcagggacg
     9841 agttcgggcc cggcctgcgg cacgaccgcc cgggcctcct ctcgatggcg aacgccggcc
     9901 cgaacaccaa tggctcgcag ttcttcatca ccgaggtccc gaccccctgg ctcgacggca
     9961 agcacgcgat cttcggcgag gtgttcgaag ggctcgatct cgtggcggac atcgcgcggg
    10021 cgggcaacgg cgcggtccgg atggagcgcg tcgaggtgta ccgcgactga gcgggactcg
    10081 cgggagggga tgagcaggcc gcggcggccg cacggggcgt cgcaggcggc ggccgtgggc
    10141 gaatgcggca ccccggcgga cgcgccggaa tgtggcccgt gccttactat tcgggcctca
    10201 ctgcaaggca acccggcggg ctgcccggcg cgaagccccg accacgggct cggccgggcg
    10261 acatccgccc agctcccgat tcgatgaagc acccggccct cctcctcgtc ctcgctctcc
    10321 tcgtcgtccc gtcccccgtc cgcgcgcagg acgcggcgcc gacggacgag actctgtccg
    10381 gcatcacgat ccgcaacatc ggccccgcgc tgatgtccgg gcgcatcgcc gacatcgctg
    10441 tccatccggc cgatttcggc acgtggtacg tggccgtcgg ctcgggcggc gtgtggaaga
    10501 ccgtgaacgc gggcaccacg tgggagccgc tcttcgacgg gcaaccctcc tactcgatcg
    10561 ggtgcgtcac gctcgacccg tccaatcccg agatcgtctg ggtgggaacc ggcgagaacg
    10621 tgggcggccg gcacgtggga ttcggcgacg gggtgtaccg cagcatggac ggcggcgccc
    10681 actgggagag catggggctc gccgcatccg agcacgtgtc gaagatcctg gtgcacccgg
    10741 aagactcgaa cacgatctgg gtggcctcgc aggggccgct ctggtccgct ggcggggacc
    10801 gcggcgtctt ccggtccacg gacggcggcg cgacctggac gcgggtgctc ggcgacgacg
    10861 agtgggtggg cgccaccgat ctcgtgatgg acccgcgcga ccccgaccgg ctctacgccg
    10921 ccacgtggca gcggcaacgc acggtcgctt cctacatggg cggcggaccc ggtacagcga
    10981 tctaccggtc cgaggacggg ggcgagacgt ggcagaagct cgcgaacg
//
LOCUS       DBNHQZ010000209         5960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3178278, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000209 DBNHQZ010000000
VERSION     DBNHQZ010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3178278"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..623)
                     /locus_tag="AC16YU_05580"
     CDS             complement(48..623)
                     /locus_tag="AC16YU_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6064727.1"
                     /translation="MADSHAVTRLLEAYRSGDSGASDQLFELVYGELHAMARRQLRFR
                     RPGDTINTTGLVHEAYLKLFDRSDADWNDRAHFFAVTARAMRQILVDYARMAHAQKRG
                     GDAHRTELDSGVLFEEARPLDLLDLEDAMKKLAELNPRMAQIVEMRYYGGMEIQEIAA
                     VLDVSSRTVDRDWYKAKSFLYLALTEGTDAG"
     gene            932..4474
                     /gene="smc"
                     /locus_tag="AC16YU_05585"
     CDS             932..4474
                     /gene="smc"
                     /locus_tag="AC16YU_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6064728.1"
                     /translation="MSKLELHGFKSFAQRTVVHFSPGVTVVVGPNGCGKSNIVDAVRW
                     VIGEQRARILRSEKMENVIFNGTLTRRALGMAEVLLTVENTRGILPTEYSEVTIGRRL
                     YRSGESEYLLNGVQCRLKDITDLFMDTGMGAGAYSVIELKMIEDLLSENAQDRRHLFE
                     EAAGITKYKVRRAQTLRKLDGTQADLVRVRDLTEELDRQVRSLERQARKAGRFRALQE
                     ELRALETALAQSEYDRLREQAESARRERRLLADRLEAEGAHLAEAEARHEAHRTEHIE
                     REQRVTAEQGRQAAQVDALRKLESDERITRERLAVAGRDLDRLAGEARAALERRTALE
                     ARLDTLAREVDDAVPALASADTALDEARTGRDAASGRSLAHRQQLDALVRAERAAADT
                     RADAQRAMDRVTSRLEMLRDELRGIDERLEPARDAVSEALRAADAARDALESAAAVRT
                     VARAELDGLARERTALEARLERTLEALRDAQRRQDAVGAEVALLESLLSSWDDFAESV
                     QHLASERDWTTGDLRTVADLLSCDETHRPALEAAIGAFADCVVVATEDEARRAIGRLR
                     TDDKGRATFLVLERLPAAGPAPDMALRPLAAVVRVADPAAAPLVPVLLRGAYLVDHLD
                     AALVAARRAGSGRFFTPTGEWVDHDGIVQGGGERAGRSPASGRIGRREQLADLVARLS
                     EAEAAVHAAQAQADSARAALEALPVGRAEEALRLAEQQVSSAESELARLTAEHAAAVR
                     RAADIEQRRGQLIAAAGGGEEEIATRTAALDEAERLLLRARQDREQVEARSAATDEDL
                     RSAETAFNDARVAAVEARNRHDNLKRELDRAREDLARLERERIDREERIAALRTSREE
                     SEAHIARLADDIRAARSGLGEINQVVDAARDVLSETKVTISAIESELRELRRSREATL
                     REENLLAIRETEVTTRIEDLVAGIREDFEVDLTTTRFEIPEGFVEVTARTEASELRSK
                     IRGLGPVNALALDSFEEERERLEFMRTQLRDLESAEATLLETITEINTTASERFRTTF
                     EEIRKNFSGLFATLFGEEATADIVLENPDDLLESAIEIMAKPRGKRPSVLAQLSGGEK
                     TLTAIALLFAIYLVKPSPFCILDEVDAPLDDANVDRFMTLIRSFADTTQFILVTHNKR
                     TMEAADRMYDITMQEQGVSRLVGVRFDEVLEAVA"
     gene            complement(4550..4942)
                     /locus_tag="AC16YU_05590"
     CDS             complement(4550..4942)
                     /locus_tag="AC16YU_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064729.1"
                     /translation="MKALALDRPLSYFEHVDSLYEEQGRLVDEAAGRIRLMAQAAESA
                     GVRFEVVVLPFEAQLRSGGRTRPHEILRRALAPDSIAVLDPSAHLAASTGSPTDLYLY
                     GDGIHFSSRGHAVLADFVLERLGPVERR"
     gene            complement(4939..5514)
                     /locus_tag="AC16YU_05595"
     CDS             complement(4939..5514)
                     /locus_tag="AC16YU_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064730.1"
                     /translation="MAAPLRALLVNLASVLGVLAVLEIGVRTLGPSIPPVGTDRLLIA
                     DSVYGASPGLRPGAAGYSNGAWFEVGSRGFVSYSKPVSDGTPGWLFLGDSVTMGLGVD
                     PDSTFAGRLATRLEGIDIWNPSLIGYAVEDYHRIFDALIGADDLERVTLFWCLNDLYS
                     SGGGVIRAAAYGSPADGSSGPFSVTTGRTTW"
     gene            complement(5596..>5960)
                     /locus_tag="AC16YU_05600"
     CDS             complement(5596..>5960)
                     /locus_tag="AC16YU_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR6064731.1"
                     /translation="GLGLVDVRAMFGIRAIRFQKRVEVVVDLHHWDRSVEYTRLGMVD
                     ETRDILGVELPLVKLPITPGKNVTVLCEVIAMNHLLRHYGYDPAEVFAERLAQRIREK
                     TEGGAPARGIEYFEHDYE"
BASE COUNT          969 a         2122 c         2038 g          831 t
ORIGIN      
        1 accgttccgg agtaatgtcc ttcatgccgc gtcggtatca gaggggatca accggcgtcc
       61 gtgccctcgg tcagcgcaag atagaggaag gacttggcct tgtaccaatc gcggtccacg
      121 gttcgcgacg aaacatcgag cacggccgcg atctcctgga tctccatgcc gccgtagtag
      181 cgcatctcga cgatctgcgc catgcgcggg ttgagctccg ccagcttctt catcgcgtcc
      241 tccaggtcca gcaggtcgag cggcctcgcc tcctcgaaca gcacgcccga atcgagctcg
      301 gtccgatgcg cgtctccccc ccgtttctgc gcatgggcca tgcgggcgta gtcgaccagg
      361 atctgccgca tcgccctcgc cgtcaccgcg aagaagtggg cccggtcgtt ccagtccgcg
      421 tcgctccggt cgaagagctt caggtaggcc tcgtgtacga gcccggtcgt gttgatcgtg
      481 tcccccggcc gccggaagcg cagctgccgc cgcgccatcg catgcagttc cccgtagacc
      541 agttcgaaca gctggtccga cgccccggag tcgccgctcc ggtaggcttc gagcagtcga
      601 gtgacggcgt gggaatcggc catcggggtt caggagagcg tggaatcgcc ttggggcggg
      661 tgggaattaa ccatgccccg gtgggaaatc aagggggtgc ggcccggtcc gggctccgcc
      721 cgcgcgtggc ggcatggacg tttcggttgg caataacggc attagtcgat cggagcctgg
      781 cgaacccctt cccgtaaccc gtccaaagcc cgacgtttac gtcgatcgaa acatacgtcg
      841 cgatttgtgg aaaacctgcg ttgcaaaacg gccatcgcct tcgttttatt agtaggtcca
      901 actcgcccga atccgcggtc cgcccctgta cctgagcaag ctcgaactgc acggtttcaa
      961 gagcttcgcg cagcgtaccg tcgtgcattt ctcgcccggc gtcacggtcg tcgtcggccc
     1021 gaacggttgc gggaagtcga acatcgtcga tgccgtccgc tgggtcatcg gggagcagcg
     1081 cgcgcgcatc ctgcggtccg agaagatgga gaacgtcatc ttcaacggca cgctcacgcg
     1141 gcgcgccctc gggatggccg aggtcctgct caccgtcgag aacacccggg gaatcctgcc
     1201 cacggagtac tcggaggtga ccatcggccg gcggctgtac cgctccggag agtcggaata
     1261 cctgctgaac ggggtccagt gcaggctgaa ggacatcacc gacctgttca tggacaccgg
     1321 catgggcgcc ggcgcctact cggtcatcga gctgaagatg atcgaggacc tcctctcgga
     1381 gaacgcccag gatcggcgac acctgttcga ggaggccgcc ggcatcacga agtacaaggt
     1441 gcgccgggcc cagaccctgc gcaagctgga cggcacgcag gcggacctgg tccgcgtccg
     1501 cgacctgacc gaggaactgg atcgccaggt gcgctccctc gagcggcagg cgcggaaagc
     1561 cgggcgcttc cgggcgctgc aggaggagct gcgcgctctc gagaccgccc ttgcccagtc
     1621 cgagtacgac cgactgcgtg aacaggccga aagcgcgcgc agggagcgcc gccttctcgc
     1681 ggaccgcctc gaggccgagg gcgcgcacct cgccgaggcc gaggcccgcc acgaggccca
     1741 ccggaccgag cacatcgaac gcgagcagcg cgtgacggcg gagcagggtc gacaggccgc
     1801 gcaggtggac gcgctccgga agctcgagtc cgacgagcgg atcacgcggg agcggctcgc
     1861 cgtggccggg cgggacctgg accggctcgc gggagaggcc cgggcggcgc tggaacgtcg
     1921 cacggcgctc gaggcccgcc tggacactct cgcccgcgag gtggacgacg ccgttcccgc
     1981 gctcgcgtcc gcggacacgg cgctcgatga agcccgcacc ggccgcgacg ccgcctccgg
     2041 ccggtccctc gcgcaccggc agcagctgga tgccctggtg cgcgccgagc gcgcggcagc
     2101 cgacacccgc gccgacgccc agcgcgccat ggaccgcgtg acgagccggc tcgagatgct
     2161 ccgggacgag ctccgcggca tcgacgagcg gctcgagccc gcccgtgacg cggtgtcgga
     2221 ggcccttcgc gcggccgacg cggcccgcga cgccctggag tccgccgcgg ccgtccgtac
     2281 cgtcgcccgc gccgagctcg acggcctggc ccgggagcgt acggcgctcg aggcccggct
     2341 tgaacgcacc ctcgaggcgc tccgcgacgc ccagcggcgc caggatgccg tgggcgccga
     2401 ggtggcgctc ctcgagagcc tcctctcgtc ctgggacgac ttcgccgagt ccgtgcagca
     2461 cctggcctcc gagcgtgact ggaccacggg cgatctgcgg acggttgcgg acctgctctc
     2521 ctgcgacgaa acgcaccgcc ccgccctcga ggcggcgatc ggtgccttcg ccgactgcgt
     2581 ggtcgtggcc accgaggacg aggcgcggcg ggccatcggc cgcctgcgca cggacgacaa
     2641 gggccgggcc actttcctgg tgcttgagcg gcttcctgcg gcaggccccg ccccggacat
     2701 ggcactgcgg ccgctcgccg cggtggtccg agtggccgat cccgccgccg ccccgctcgt
     2761 gcccgtcctg ctccggggcg cgtacctcgt cgaccacctg gacgccgccc tcgtggccgc
     2821 gcgccgggcc ggctccggcc ggttcttcac gccgacgggc gagtgggtcg accacgacgg
     2881 gatcgtacag ggaggcggtg agcgcgcggg acgctcgccg gcgtcgggtc gcatcggccg
     2941 tcgcgagcag ctcgcggatc tcgtcgcgcg gctctcggag gccgaggcag ccgtccacgc
     3001 cgcccaggcc caggccgact ccgcgcgcgc ggcgctcgag gccctgcccg tcggtcgcgc
     3061 ggaggaggcc ctgcggctcg ccgagcagca ggtgtcctcg gccgagagcg aactcgcccg
     3121 gctcaccgcc gagcacgcgg ccgccgtccg ccgcgcggcc gacatcgagc agcgccgcgg
     3181 ccagctcatc gccgccgcgg gcgggggcga ggaagagatc gccacgcgga ccgccgcgct
     3241 cgacgaggcc gagaggctgc tgctgcgggc ccggcaggac cgggagcagg tcgaggcgag
     3301 gtccgccgcg acggacgagg accttcgcag cgccgagacc gcgttcaacg acgcgcgcgt
     3361 cgccgccgtc gaggcccgca accggcacga caacctgaag cgcgagctgg accgggcgcg
     3421 ggaggacctt gcccgcctcg agcgcgagcg gatcgaccgg gaggagcgca tcgccgcgct
     3481 ccggacctcc agggaggagt ccgaggcgca catcgccagg ctcgccgacg acatccgcgc
     3541 ggcgcgttcc ggactcggcg agatcaacca ggtcgtcgac gccgccaggg acgtgctgtc
     3601 cgagaccaag gtgaccatct ccgcgatcga gagcgagctg cgtgagctcc gccgctcgcg
     3661 cgaggccacc ctgcgcgaag agaacctgct cgccatccgg gaaaccgagg taacgacgcg
     3721 tatcgaggac ctcgtggccg gaatccggga ggacttcgag gtggacctca ccaccacgcg
     3781 gttcgagatc cccgagggct tcgtcgaagt cacggcgagg acggaagcct ccgagctgcg
     3841 ctccaagatc cgcggcctgg gcccggtgaa cgcgctcgcc ctggacagct tcgaggagga
     3901 gcgcgagcgc ctggagttca tgcgcaccca gctccgcgac ctggagagcg cggaagccac
     3961 gctcctcgaa acgatcacgg agatcaacac gaccgcgtcg gagcgcttcc ggaccacgtt
     4021 cgaagagatc cgcaagaact tctccggcct gttcgccacg ctcttcggcg aggaggcgac
     4081 cgccgacatc gtcctcgaga atcccgacga cctcctggaa tcggccatcg agatcatggc
     4141 caagccgcgc ggcaagcgtc cctcggtgct ggcccagctc tcgggcggcg agaagacgct
     4201 gacggccatc gcgctgctct tcgcgatcta tctcgtgaag ccgagcccct tctgcatcct
     4261 ggacgaggtc gacgccccgc tcgacgacgc caacgtggac cgcttcatga cgctgatccg
     4321 ctcgttcgcc gacaccacgc agttcatcct cgtcacgcac aacaagcgga cgatggaagc
     4381 cgccgaccgc atgtacgaca tcacgatgca ggagcagggg gtgtcgaggc ttgtgggcgt
     4441 gaggttcgac gaggtgctgg aggccgtggc ctgaacggat cggctatcct gaattgcgac
     4501 tgcaggacag ccttccaggg gtccggcgca cggtgatcat ccgcgctgtt cagcgacgtt
     4561 cgaccggccc gaggcgctcc aggacgaagt cagccagcac cgcgtggccc ctggacgaga
     4621 agtgaatccc gtcgccgtac aggtacaggt cggtcgggga cccggtcgac gccgccaggt
     4681 gcgcgctggg atcgagaacc gcgatcgaat ccggggccag cgcgcggcgc aggatctcgt
     4741 gcggccgggt gcgtccgccc gaccggagct gggcctcgaa agggagcacg acgacctcga
     4801 accgcacccc ggcgctttcc gcggcctgcg ccatgagacg gatcctcccg gccgcctcgt
     4861 cgacgagccg tccctgttcc tcgtacagcg agtcgacgtg ttcgaaatag ctgagcggcc
     4921 ggtcgagagc gagcgccttc accaggtggt acgtccggta gtgacgctga acggcccgga
     4981 ggagccgtcc gccggcgagc cgtatgcggc tgcccggatc accccccccc cgctcgaata
     5041 gagatcgttc aggcaccaga acagcgtgac gcgctccagg tcgtcggccc cgatgagcgc
     5101 atcgaagatc cggtggtaat cctcgaccgc gtaaccgatc agcgacgggt tccagatgtc
     5161 gatcccttcc agccgggtcg ccaggcgtcc cgcgaacgtg gaatccggat cgactcccag
     5221 gcccatcgtg accgagtcgc cgagaaaaag ccatcccggc gtgccgtcgc tgacgggctt
     5281 cgaataggac acgaagcccc ggcttcccac ttcgaaccag gcgccgttgc tgtagccggc
     5341 tgcgccgggc cggagtccgg gcgatgcgcc gtatacactg tccgcgatga ggagccggtc
     5401 cgttccgacg ggtggaatgg acggaccgag ggtgcgaacg ccgatctcca gcaccgccag
     5461 gaccccgagg acggacgcca gattgaccag cagggcgcgg agcggagcag ccatgtcaga
     5521 aacgtatcgg cgaccggacg ataggatacc accctcccga gcagccgcat acctcgacga
     5581 cgccgagccg cccccctact cgtaatcgtg ctcgaagtac tcgatcccgc gcgccggcgc
     5641 tcccccttcc gtcttctccc ggatccgctg ggcgagccgc tcggcgaaga cctcggccgg
     5701 atcgtagccg tagtgccgga gcaggtggtt catcgcgatc acctcgcaga gcaccgtgac
     5761 gttcttcccc ggggtgatcg gaagcttgac gagcgggagc tccacgccca ggatgtcgcg
     5821 cgtctcgtcg accatcccca ggcgggtgta ctcgaccgag cggtcccagt ggtgcaggtc
     5881 cacgacaact tcgaccctct tctggaagcg tatcgcgcgg atcccgaaca tcgcccggac
     5941 atcgacgagc cccagacccc
//
LOCUS       DBNHQZ010000210        11591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1690098, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000210 DBNHQZ010000000
VERSION     DBNHQZ010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1690098"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC16YU_05605"
     CDS             complement(<1..517)
                     /locus_tag="AC16YU_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064732.1"
                     /translation="MIRVENLFKSFEGRAVLTDVSLEIPEGKTTAIIGRSGSGKSVLM
                     KHIIGLLRPDRGRVTVDGVDINRINYGELRRVRRQFGVLFQGGALFDSMDSFENVAFP
                     LRTFTTMSDAEIRREVEGCLAMVDLPDAGPKMPADLSGGMRKRVALARSLALDPELLC
                     LDEPSSGLDPVN"
     gene            complement(521..1282)
                     /locus_tag="AC16YU_05610"
     CDS             complement(521..1282)
                     /locus_tag="AC16YU_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR6064733.1"
                     /translation="MTHKRPFVLAPAAALGSYSLLLFHAFSSLGEFASYRKNVLQQMV
                     RVGVESLPIVALAAAFSGAVTTVQTAYQLVSPFIPKSIIGAIVVPSMILELGAVVTGF
                     ILAGRVGARIAAELGTMRVTEQIDALEAMGLNSIGYLIVPRVLAGIVMFPVLYIAASF
                     IGIAGGLFVGHAGGFVPAGEFIDGAREFFQPFDPVFGLIKSFVFGFIITSVSCFKGYN
                     TLGGAEGVGRATTQAAVISCVYILVADLVLAATLL"
     gene            1506..2855
                     /gene="radA"
                     /locus_tag="AC16YU_05615"
     CDS             1506..2855
                     /gene="radA"
                     /locus_tag="AC16YU_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6064734.1"
                     /translation="MARPRSRFVCQACGHESARWTGQCPACGEWNTQVEEVVPGGSAA
                     PGRTGPMPRTMLLGEVSPTTNGRLRTGVEEFDRVMGGGIVTGSVTLVAGDPGIGKSTL
                     MTEVAALVAGRGVLYVCGEESPEQVRLRADRLDVDAAAVHLLPETDADAVVRAADALR
                     PAVLIVDSIQTLYRGDIQSAPGSVAQVRECTATIMRHAKQSGTAVFVVGHVTKEGTIA
                     GPRVLEHMVDTVLHLEGDRHHAFRVLRAVKNRFGSTNEIGVFEMQGTGLRPVANPSEI
                     FLSERSEDASGSAVVCTLEGTRPVLAEIQALVTPTSYPSPQRTATGFDGRRLQMLLAV
                     LEKREGLRLSTQDVFVNVAGGLRLTEPAVDLGIVMAVVSSFKDRPLPRHTVVLGEVGL
                     GGEIRSVSQLPLRLREAGKLGFRTAVVPAHGLNGTAEGGLPETVGVSRLSEAADRLF"
     gene            complement(2859..3557)
                     /gene="rpiA"
                     /locus_tag="AC16YU_05620"
     CDS             complement(2859..3557)
                     /gene="rpiA"
                     /locus_tag="AC16YU_05620"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR6064735.1"
                     /translation="MSDAAKQRVGERVAAWVVDGMVVGLGTGSTAVRAIEAVGRRVAE
                     ESLRIVGVPTSFFAERAARGLGIPLANLDEVERVDLAFDGADEVSPALDLIKGRGAAH
                     SREKVVAAASDRFVVLVDESKLVDRLGTRFPVPVEVMPMAVTPVMRAMERLGGRPSLR
                     IGERKDGPVVSDQGFWIVDVSFDGIDDPAAMDRAIRSVPGVLDHGLFIGLATDVLVGR
                     ADGSVEHRTRGRVD"
     gene            complement(3627..4856)
                     /locus_tag="AC16YU_05625"
     CDS             complement(3627..4856)
                     /locus_tag="AC16YU_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064736.1"
                     /translation="MAIFEAFRMALESLRGHKLRSSLTLLGMVIGVFAIIVSVTAVKV
                     IDVYFKESLDFLGSSTFTISRHPMIQTGPLDRSQRNRPPITIEEVERLRRTVDLPVTI
                     SVMDGFDLGTVKYADRESEPNIQFLGGDENMLGNYSYELESGRFFTESDLQFARPVTV
                     IPRGIADFLFPNETPLGKQIRFKGLRFEVIGVLKDKGSFLGFNQDNRIFVPLTRAYLL
                     YGGTRRDAGSISVRVPDMTMMNAAIEETSGRLRVIRKVEPGEDNNFEVSTNQSLQQIF
                     EAFTGVLTTGGAGIGLIALLAAGIGIMNIMLVSVTERTREIGIRKSVGARRRDIMRQF
                     LLEAFFLCQIGGLFGILLGALVGNGVALYFDISAAFPVGWAIAAVVMVTGVALVFGGY
                     PALKAARLNPIDALRYE"
     gene            complement(4860..6095)
                     /locus_tag="AC16YU_05630"
     CDS             complement(4860..6095)
                     /locus_tag="AC16YU_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064737.1"
                     /translation="MHLHFEFLESVRIALRAIRVNKMRAVLTTLGIVIGIVSVTAMAT
                     VVSGIEQGFEQEMSSLGVDVVYIERWPWADGPGSRWWEYINRPRIQHDIAEAIIARSR
                     YADAVTSVVRTGRSVSAGGQTLNGITIGGVTPAYTRVFTVDLAEGTFVSEMDERTARR
                     VAVIGAKIADELFPVGDPIGKAIRIQGQEYRVVGVLVRKGQGADSAQSEDFRVFVPFT
                     AFRSQFGTRWRDVSVQVRIASGVELEEAKDEIRGIVRLARRLDAKEADNFEINDQVTL
                     RAQIEPIKTAIYSIGIGLTALSLLVGGIGVMNIMFVSVKERTREIGIRKAVGARRRTI
                     LMQFLVEAVAVSLLGGVIGVLLSIPLALLIKAVMPAHLGPGIVAIAFGICLFIGTVFG
                     LAPAWTAARAEPIDALRYE"
     gene            6234..6392
                     /locus_tag="AC16YU_05635"
     CDS             6234..6392
                     /locus_tag="AC16YU_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HIR6064738.1"
                     /translation="MGKLKILGEFWQFLRIRKKYWLAPIVVVLLLLSLLIVVTQGSAL
                     APFIYALF"
     gene            complement(6402..6848)
                     /locus_tag="AC16YU_05640"
     CDS             complement(6402..6848)
                     /locus_tag="AC16YU_05640"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6064739.1"
                     /translation="MCELHVAGLVIREVGRESMDTIRELNRVIFEEERIINTFDRPHL
                     LMLLAEIDEEPVGFKIGYRENRFVYYSAKGGVLEEYRRTGIARAMMQELVDRIRPYGY
                     RRLAFDTFPNRHPGMTVLALQDGFRVVMADYNHTYRDFRLRFEKPI"
     gene            complement(6841..8868)
                     /locus_tag="AC16YU_05645"
     CDS             complement(6841..8868)
                     /locus_tag="AC16YU_05645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6064740.1"
                     /translation="MARRFILKAEPGPDLDRFRIDYPGSLNPQQLAAATAGGGASLVV
                     AGAGTGKTRTLVYRVAYLVESGVPPEEIVLLTFTRRASREMLARASALLDGRCERVQG
                     GTFHSFCVGILRRHAPRLGFPNRFTILDASDAADVIDVLRGAMDLPKGQRFPRKRTLQ
                     ALFSAVVNQALPLGDVLGERYPQFAELEPVLAELMGRYEGYKRAHGLMDYDDLLRRTL
                     ELFRDHEDVGRTVAAECRHVLVDEYQDTNRMQAALVRAFSSVHGNVMAVGDDAQSIYR
                     FRGADFRNILAFPDEFPGTRVFKLEHNYRSTQSILDLANHLIVQARHKFDKQLFTERT
                     GGDLPAIVTAADARFESRFVCQMLLELREQGVPFRDMAVLFRSGHNSYELEVELNRRN
                     IPFVKYGGLKLSEAAHVKDVLAHLRVLENPRDAVSWNRILLLLEGIGPKTAGQVIEWL
                     LAAGDDALVLPERPFSPRYADALASLFELLRRIRSDSLPLEQQVAAVLDYYEPILKRQ
                     YYEDYPKRLQDLEHFAGVVGSHADRASLLNALALDPIELTAVDVEPLEEDEAPLVLST
                     IHSAKGLEFRAVFVIHALEGVLPSAYAVGRDDDLDEELRLLYVAVTRAADHLFLSWPM
                     VQYRRYQGQYLTSPSRFVAEVPDRLLEPWSLSEEPALPFELPPASGGIADV"
     gene            9088..9939
                     /locus_tag="AC16YU_05650"
     CDS             9088..9939
                     /locus_tag="AC16YU_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6064741.1"
                     /translation="MGKVIAVANQKGGVGKTTTAVNLATSLAAVEYRTLLVDFDPQAN
                     GTSGVGVDSRTVASSSYEVLIGSVALADAVLATEMPFLDLVPSHINLVGAEIEMVDVP
                     ERERILRRALAGVRDRYDFVVIDCPPSLGLLTLNALSAASSVLIPVQAEYFALEGLGQ
                     LLNTIKLVRQHLNPDLEIEGVLVTMFDVRLRLANQVAGELRKYFGDRVFQTVVQRNVR
                     LSEAPSFGKPVLLYDATSVGARNYIALAREVIARSTVSADVVNGIGAPGFTARPATPG
                     SHRNGHT"
     gene            9959..10906
                     /locus_tag="AC16YU_05655"
     CDS             9959..10906
                     /locus_tag="AC16YU_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6064742.1"
                     /translation="MTKGKVALGKGLGALLPGGDEARPDPMPVDVPATRLYDFQDRAR
                     VGRVSDVEIDQIDPNPFQPRQSFDEGALEELAASIRQLGVIQPITVRTVEHGRFQIIS
                     GERRVRAARRAGLRTIPSYVREADTEAMLEMALVENVQREELNPIEVAVGYQRLIDEC
                     RLKQEEVADRVGKNRTTVTNFLRLLRLPPPVQACLRDGSLSVGHARALIPVEDEKTQL
                     QMLAKIVDGGLSVRAVEDLVKAWHRRRDRSERDAPEPMQPARSTRDMLQLRALTDRLR
                     SSLGTRVQIRPGASGEGGRIEVAYYSDDDLERLIDLMGS"
     gene            10903..11556
                     /locus_tag="AC16YU_05660"
     CDS             10903..11556
                     /locus_tag="AC16YU_05660"
                     /inference="COORDINATES: protein motif:HMM:NF040144.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HIR6064743.1"
                     /translation="MSIPRPSRRAPGRVVAAAVVLVAFAPGSGTLRARPVALPAAADT
                     TTGARGRSPRGALLRSVVVPGWGQVYNGQPLKAPVIVAALGGLAYAVVTLDGDYRLYR
                     EAYQYKAFQELVDRGELTENPREGYADSYDRVAADVGAVSSGPLRAQRDALRRNRDLS
                     ILGIGLVWAISALDAYVSAHLLDFDVGEDLTLRVRPEIAPGTGVVPAPALVWRPGRR"
BASE COUNT         1865 a         4009 c         3931 g         1786 t
ORIGIN      
        1 cgttgaccgg gtccaggccc gagctcggct cgtccaggca caggagctcc ggatcgagcg
       61 cgaggctgcg ggcgagcgcc acgcgcttgc gcatgccgcc ggagagatcc gccggcatct
      121 tcgggcccgc gtcgggcagg tccaccatgg ccaggcagcc ctcgacctcg cgccggatct
      181 cggcgtccga catcgtcgtg aacgtccgca gcggaaacgc cacgttttcg aacgagtcca
      241 tcgaatcgaa aagcgcgccg ccctggaaca ggacgccgaa ctgccggcgc accctgcgga
      301 gctcgccgta gttgatccgg ttgatgtcga cgccgtcgac ggtcacgcgc ccccggtccg
      361 gccgcagcag cccgatgatg tgcttcatga gcacggactt gcccgatccc gaacggccga
      421 tgatggccgt cgtcttgccc tccgggatct cgagcgagac gtccgtgagc accgcccggc
      481 cctcgaagct cttgaacagg ttctcgacgc ggatcatgga tcagagcagt gtggccgcga
      541 ggaccaggtc cgcgaccagg atgtacacgc agctgatgac cgcggcctgg gtggtcgcgc
      601 gcccgacgcc ctcggccccg ccgagcgtgt tgtagccctt gaagcaggag accgacgtga
      661 tgatgaagcc gaagacgaac gacttgatga gtccgaatac ggggtcgaac ggctggaaga
      721 actcccgcgc cccgtcgatg aactcgcccg ctggcacgaa ccccccggcg tggcccacga
      781 agagccctcc ggcgatgccg atgaagctgg ccgcgatgta gagcacgggg aacatcacga
      841 tgccggcgag cacccgcggc acgatcaggt agccgatcga gttcagcccc atcgcctcca
      901 gcgcgtcgat ctgctcggtg acccgcatgg tgccgagctc ggccgcgatg cgggctccga
      961 cccggcccgc gaggatgaag cccgtgacca cggcaccgag ctccaggatc atcgacggca
     1021 cgacgatcgc cccgatgatg ctcttcggga tgaagggcga cacgagctgg taggccgtct
     1081 gcaccgtcgt caccgcgccc gagaacgccg cggcgagggc cacgatgggc agcgattcca
     1141 cgcccacgcg gaccatctgc tggagtacgt tcttccggta cgaggcgaac tccccgagcg
     1201 acgagaacgc gtggaacagc agcaggctgt agctgcccag cgccgcggcc ggcgccagga
     1261 cgaatggtcg cttgtgcgtc atcggagagg atttcggacg tgccgatccg ccgcccgggt
     1321 ggggacctcg cgggcgccgg cccggacacc tgccgggcgg gcgcgccaag gtacgcagcg
     1381 gtccattttc atgccgggcg gattccggag ggggacgggc gaaaggggcc cgtggcaacg
     1441 gcatgtgtca cgcccttgtc gcgcgacgcc cctatactgc cgcaggaatt ccataacagg
     1501 taaccatggc ccgcccccgc tcccgcttcg tctgccaggc ctgcggacac gaatccgccc
     1561 gctggacggg ccagtgcccc gcctgcgggg agtggaacac ccaggtcgag gaggtcgtgc
     1621 ccggcggatc cgccgccccg ggccgcaccg gcccgatgcc ccgcacgatg ctgctgggcg
     1681 aggtctcccc gaccacgaac ggccggctcc ggaccggcgt cgaggagttc gatcgcgtca
     1741 tgggcggtgg aatcgtcacc ggttccgtga cgctggtcgc cggtgatccg ggtatcggga
     1801 agagcacgct gatgacggag gtggccgcgc tcgtggcggg gcggggagtc ctgtacgtct
     1861 gcggcgagga gtcgcccgag caggtccgcc tccgcgccga ccgcctggac gtcgacgcgg
     1921 cggccgttca tctcctgccc gaaaccgacg cggacgccgt ggtccgggcc gcggacgcac
     1981 tgcgccccgc cgtgctgatc gtggactcca tccagacgct gtaccgcggc gacatccaga
     2041 gcgcgcccgg gtcggtggcc caggtgcgcg aatgcacggc aacgatcatg cggcacgcca
     2101 agcagtccgg gacggccgtg ttcgtcgtgg gtcacgtgac caaggagggc acgatcgccg
     2161 ggccccgcgt gctggaacac atggtggaca ccgtgctgca cctggaaggg gatcggcacc
     2221 atgccttccg tgtgcttcgc gccgtgaaga accggttcgg gtccacgaac gagatcggcg
     2281 tcttcgagat gcagggcacg ggtctcaggc ccgtcgcgaa cccgagcgag atcttcctct
     2341 cggagcggag cgaggacgcg agcggatcgg cggtggtctg cacgctcgag ggcacccgtc
     2401 ccgttctggc cgagatccag gcgctggtca cccccacctc gtacccatct ccccagcgga
     2461 cggccacggg gttcgacggt cgacggctgc agatgctgct cgccgtgctc gagaagcggg
     2521 agggtctccg gctctcgaca caggacgtgt tcgtgaacgt ggccggaggg ctccgcctaa
     2581 cggagcccgc ggtcgacctg gggatcgtga tggcggtcgt ctcctcgttc aaggatcgtc
     2641 cgctgccacg gcacacggtg gtcctgggcg aggtcggact cggcggcgag atccgctcgg
     2701 tgtcccagct gccgctgcgc ctgcgggagg cgggcaagct cggtttccgt acggccgtcg
     2761 tgccggcgca cggcctgaac gggaccgcgg aaggggggct tcccgaaacg gtcggcgtgt
     2821 cacgcctgag cgaggcggcg gaccgtctgt tctgagcttc agtcgactcg gccgcgcgtt
     2881 cggtgctcca ccgagccgtc cgcccgcccg acgagcacgt cggtggccag gccgatgaac
     2941 agcccgtgat ccaggacccc ggggacggag cgtatggccc ggtccatcgc ggccggatcg
     3001 tcgataccgt cgaaggagac atccacgatc cagaacccct ggtccgatac gacgggcccg
     3061 tccttcctct ccccgatgcg cagcgagggg cggcctccga ggcgctccat cgcgcgcatg
     3121 accggggtga ccgccatcgg catcacctcg accggaacgg ggaaccgggt cccgagccga
     3181 tccacgagct tcgactcgtc caccaggacc acgaaccggt cggatgccgc cgccaccacc
     3241 ttctcccggg aatgcgcggc cccgcgcccc ttgatcaggt cgagcgccgg gctcacctcg
     3301 tcggcaccat cgaaggccag gtccacccgc tcgacctcgt cgaggttcgc gagcgggatg
     3361 cccagcccgc gcgccgccct ctcggcgaag aaggacgtgg gcactccgac gatccgcagg
     3421 gattcctccg ccacccggcg acccaccgcc tcgatggcgc gtacggccgt ggaccccgtg
     3481 cccagaccga cgaccatgcc gtccaccacc catgcggcga cccgctcgcc gacccgttgc
     3541 ttggccgcgt ccgacatgcc tcctccgatc gagtgcgtga cgagcggatg ttccggctcc
     3601 cgcgcgagag atgccagtgg agcgcgttac tcgtagcgaa gcgcgtcgat cggattcagg
     3661 cgcgcggcct tcagcgcggg atacccgccg aacaccaggg cgaccccggt gaccatcacc
     3721 acggccgcaa tggcccaccc gaccggaaag gcggccgaga tgtcgaagta cagcgccacg
     3781 ccgttgccga cgagggctcc gagcaggatg ccgaagaggc cgccgatctg gcagaggaag
     3841 aaggcctcca ggaggaactg ccgcatgatg tcacggcgcc gcgcgcccac ggacttccgg
     3901 atgccgatct cccgcgtccg ctccgtcacc gagacgagca tgatgttcat gatgccgatg
     3961 ccggccgcga gcagcgcgat gaggccgatg cccgctccgc ccgtcgtgag cacgccggtg
     4021 aaggcctcga agatctgctg caggctctgg ttcgtcgaga cctcgaagtt gttgtcctcg
     4081 cccggctcca ctttccggat cacccgcaat cgcccgctcg tctcctcgat ggcggcattc
     4141 atcatcgtca tgtcgggcac gcgcaccgat atgctgccgg cgtcgcgacg cgttcccccg
     4201 tagagcaggt aggcgcgcgt caggggcacg aagatccggt tgtcctggtt gaagcccagg
     4261 aagctgccct tgtccttcag gacgccgatc acctcgaagc gcagcccctt gaaccggatc
     4321 tgtttgccga gaggcgtttc gttcgggaac aggaaatccg cgatcccccg ggggatgacc
     4381 gtcaccggcc gggcgaactg caggtccgac tcggtgaaga agcggccgga ctccagttcg
     4441 tagctgtaat ttcccagcat gttctcgtcg ccgcccagga actggatgtt gggctcgctc
     4501 tcccggtccg cgtacttgac ggtccccagg tcgaagccgt ccatgacgct gatcgtgacg
     4561 ggcaggtcca ccgtgcgccg cagccgctcg acctcctcga tcgtgatcgg cggccggttc
     4621 cgctggctcc ggtcaagggg gcccgtctgt atcatcggat ggcgcgagat cgtgaacgtc
     4681 gaggacccca ggaagtcgag cgactccttg aaatagacgt cgatcacctt gaccgccgtc
     4741 accgagacga tgatggcgaa taccccgatc accatcccga gcagggtcag cgacgagcgc
     4801 agcttgtggc cgcgcagaga ctcgagagcc atgcggaagg cttcgaagat ggccattcct
     4861 tactcgtacc tgagggcatc gatgggctct gcgcgcgccg ccgtccatgc aggcgcgagc
     4921 ccgaagaccg tcccgatgaa aaggcagatg ccgaaggcga tcgccacgat cccggggccg
     4981 agatgggccg gcatgacggc cttgatgagg agcgcgagcg ggatcgagag gagcacgccg
     5041 atcacgcccc ctaggaggct caccgcgacg gcctcgacca ggaactgcat gagaatcgtc
     5101 cggcgccgcg cccctacggc cttccggatg ccgatctccc gggtcctctc cttcaccgag
     5161 acgaacatga tgttcatcac cccgatgccg ccgaccagca gcgagagggc cgtgagccct
     5221 atgccgatcg agtagatggc cgtcttgatc ggctcgatct gcgcacggag cgtgacctga
     5281 tcgttgattt caaaattgtc ggcctccttg gcgtcgaggc ggcgcgccag gcggacgatg
     5341 ccgcggatct cgtccttcgc ctcctcgagc tccaccccgc tcgcgatgcg cacctgcacg
     5401 gacacgtctc gccagcgtgt cccgaactgg ctgcggaacg ccgtgaacgg cacgaacacc
     5461 cggaaatcct ccgactgggc actgtccgca ccctggccct tgcgcacgag caccccgacc
     5521 acgcggtatt cctgcccctg gatccggatg gccttgccga tcgggtcgcc cacggggaag
     5581 agctcgtcgg cgatcttcgc gccaatcacc gccacgcggc gggccgtccg ctcgtccatt
     5641 tcggaaacga acgtgccctc ggccaggtcg acggtgaaga cgcgggtgta ggccggtgtc
     5701 acgccaccga tcgtgatccc gttcagcgtc tggccgccgg ccgaaacgga gcgtcccgtc
     5761 cgtacgaccg acgtcacggc gtcggcgtac cgggagcggg cgatgatggc ttccgcgatg
     5821 tcatgctgga tgcgcggacg gttgatgtac tcccaccacc tggaaccggg gccgtccgcc
     5881 cacggccagc gctcgatgta caccacgtcc acgccgagcg acgacatctc ctgctcgaag
     5941 ccctgctcga tcccgctcac gaccgtggcc atcgccgtga ccgaaacgat gccgatcacg
     6001 atgcccagcg tcgtgagcac ggctcgcatc ttgttcacgc ggatggcgcg cagcgcgatc
     6061 ctgacactct ccaggaactc gaaatggaga tgcacagggg ttcctcggtc aatccatgcc
     6121 cgatacgcgc acgtcccggg ggggttgcgt gcgcgcgaag cccgccgtgg gggcggtccc
     6181 cggaacccga ctcccgcacc ggcatgtacg gctccggacc acccgctcac gacatgggaa
     6241 agctcaagat tctcggcgag ttctggcagt tcctgcgcat ccgcaagaag tactggctcg
     6301 cccccatcgt ggtggttctg ctcctgctca gcctgctcat cgtcgtgacg cagggatcgg
     6361 ctctcgcgcc gttcatttac gccctgttct gagaggggcg gtcagatcgg cttttcgaat
     6421 cgcaggcgga agtcccggta ggtgtggttg tagtccgcca tcaccacgcg gaaaccatcc
     6481 tgaagcgcca gtacggtcat tcccggatgc cggttgggaa acgtgtcgaa ggccaggcgc
     6541 cggtagccgt acggccggat gcggtcgacc agttcctgca tcatggcccg ggcgatcccg
     6601 gtccgccggt actcctcgag cacgcccccc ttggcgctgt agtacacgaa gcggttctcc
     6661 cggtagccga tcttgaagcc gacgggctcc tcgtcgatct cggcgaggag catcaggagg
     6721 tgcggccggt caaacgtgtt gatgatccgc tcctcctcga agatgacccg gttgagctcc
     6781 cggatcgtgt ccatggattc gcggccgact tcccggatca ccaggcccgc tacgtgcagc
     6841 tcacacatcc gcgatcccgc ccgaagcggg tggcagttcg aagggaaggg cgggctcctc
     6901 ggaaagggac cacggctcca ggagacgatc cggcacctcg gcgacgaacc ggctggggct
     6961 ggtcaggtac tggccctggt atcggcggta ctggaccatc ggccaggaga ggaagaggtg
     7021 atcggcggct cgtgtgaccg caacgtacag cagtcgaagc tcctcgtcca gatcgtcgtc
     7081 ccggccgacc gcgtacgccg acggcagcac cccctccagg gcatggatca cgaagaccgc
     7141 ccggaattcg agccccttcg cggagtggat cgtcgacagg accagcggcg cctcgtcctc
     7201 ctcgagcggc tcgacgtcca cggccgtgag ttcgatcggg tccagcgcca gcgcgttcag
     7261 gagggacgcc cggtccgcgt ggctccccac caccccggcg aaatgctcca ggtcctgcag
     7321 gcgcttggga tagtcctcgt agtactgccg cttcaggatc ggctcgtagt aatccaggac
     7381 ggcggccacc tgctgctcga gcggcagcga atccgaccgg atgcggcgca gcagctcgaa
     7441 gagcgaggcc agggcgtcgg cgtaccgcgg cgagaacggc cgctccggca gaaccagcgc
     7501 atcgtctccc gccgcgagaa gccactcgat cacctggccg gcggttttcg ggccgatccc
     7561 ctcgagcagc aggaggatcc ggttccagga gacggcgtcg cggggattct cgagcacgcg
     7621 caggtgtgcc aggacgtctt tgacatgcgc cgcctcggag agcttcagtc ccccgtactt
     7681 gacgaacggg atgttgcggc ggttgagctc cacctccagc tcgtacgagt tgtgtcccga
     7741 ccggaagagc acggccatgt cgcggaacgg gacgccctgc tcgcggagtt cgagcagcat
     7801 ctggcagacg aagcggctct cgaagcgggc gtccgccgcg gtgactatgg ccggcaggtc
     7861 gcccccggtc cgctcggtga agagctgctt gtcgaacttg tgccgcgcct ggacgatcag
     7921 gtggttggcc aggtcaagga tcgactgggt ggaccggtag ttgtgctcca gcttgaacac
     7981 ccgcgtcccc ggaaattcgt cggggaacgc gaggatgttg cggaagtccg ctccgcggaa
     8041 ccggtagatg ctctgcgcat cgtcgcccac cgccatgacg ttcccgtgca cggacgaaaa
     8101 cgccctcacg agcgctgcct gcatccggtt ggtgtcctgg tattcgtcga cgagcacgtg
     8161 gcggcattcg gcggccacgg tgcgccccac gtcctcgtgg tcgcggaaca gctcgagcgt
     8221 gcgccggagc aggtcgtcgt agtccatgag cccgtgcgcc cgcttgtacc cctcgtaccg
     8281 gcccatgagc tccgccagga cgggctcgag ctccgcgaac tgcgggtagc gttccccgag
     8341 cacgtcgccg agcggcagcg cctggttgac gacggccgag aacagcgcct gcagcgtacg
     8401 tttgcgggga aagcgctggc ccttgggcag atccatcgcc ccccggagca cgtcgatgac
     8461 gtcggccgcg tcggatgcgt ccaggatcgt gaagcggttc ggaaaaccga gcctgggggc
     8521 atggcgccgg agtatgccga cgcagaacga gtggaacgtc ccgccctgca cgcgctcgca
     8581 gcgcccgtcg agaagcgccg aggcgcgcgc cagcatctcc cgggatgcgc gccgcgtgaa
     8641 cgtgagcagc acgatctctt ccgggggcac gcccgactcc acgaggtaag ccacccggta
     8701 cacgagggtc cgggtcttgc ccgttcccgc gccggccaca acgagggagg cgcctcctcc
     8761 ggccgtcgcc gcggccagct gctgcggatt cagcgatccc gggtagtcga tccggaatcg
     8821 gtccaggtca ggcccgggtt cggccttgag gatgaagcgg cgcgccatcg gggcatcggt
     8881 gggaaaccgt ccatctgaac gatgcagaag gccgaaaagg ttcgatcccg gcaagcccgc
     8941 ttcggggccg tcgcggtggc ttgggtgggc gcgagtccct actttcgctg ggcgggatcg
     9001 gtgcccggat tccgaaccga ggaactccgc ggtgggtcgc ggggtgtgga tggattcatt
     9061 atcgcaggga gagtggctcg tcggcgcatg ggaaaggtca ttgcggtcgc gaatcagaag
     9121 ggcggcgtgg gcaagaccac gacggccgtg aacctggcta cgtcgctggc cgccgtggag
     9181 taccgcaccc tgctcgtcga tttcgatccg caggccaacg gcacctccgg cgtgggcgtg
     9241 gactcgcgaa cggtcgcctc ttcgtcgtac gaggtcctga tcggctcggt cgcgctggcc
     9301 gacgcggtgc tcgcgaccga gatgcccttc ctggacctgg tgcccagcca catcaacctg
     9361 gtcggggccg agatcgagat ggtggacgtc cccgagcggg agcgcatcct gcgacgggcg
     9421 ctcgccgggg tgcgcgaccg ctacgacttc gtggtcatcg attgtcctcc ctcgctgggc
     9481 ctgctgacgc tcaacgcgct gtccgcggcg tcgtcggtcc tcatccccgt gcaagccgag
     9541 tacttcgcgc tcgagggcct cgggcagctg ctcaacacga tcaagctcgt gcgccagcac
     9601 ctgaaccccg atctcgagat cgagggcgtg ctcgtcacga tgttcgacgt acggctgcgc
     9661 ctggccaacc aggtggccgg cgagctgcgc aagtacttcg gtgaccgcgt tttccagacg
     9721 gtcgtgcagc gcaacgtccg gctctccgag gcgcccagct tcgggaagcc ggtgctgctc
     9781 tacgacgcga ccagcgtcgg cgcccgcaac tacatcgcgc tggcgcgcga ggtcatcgcg
     9841 cggtcgaccg tctcggccga tgtggtgaac ggcatcgggg ccccgggctt caccgcgcgc
     9901 ccggccacgc ccggctccca ccggaacggc catacctgac gcaacccgga cgcttgtcat
     9961 gacgaaagga aaagtcgcac tgggcaaggg actcggcgcc ctgctcccgg gcggggacga
    10021 agcccggccg gatccgatgc ccgtcgacgt ccccgcaacg cgcctgtacg acttccagga
    10081 ccgggcacgc gtcgggcgcg tgagcgacgt cgagatcgac cagatcgatc cgaacccgtt
    10141 ccagccgcgc cagtcattcg acgagggagc actcgaggag ctggcggcgt cgatccgtca
    10201 gctgggcgtc atccagccca tcaccgtgcg cacggtcgag cacggccgct tccagatcat
    10261 ctcgggcgag cggcgcgtgc gcgctgcccg gcgtgccggc cttcggacga tcccgtcgta
    10321 tgtccgggaa gcggacaccg aggccatgct cgagatggcg ctcgtcgaaa acgtccagcg
    10381 cgaggagctg aatccgatcg aggttgccgt cggctaccag cggctgatcg acgagtgccg
    10441 cctgaagcag gaagaggtgg cggaccgggt cggcaagaac cggacgaccg tgaccaactt
    10501 cctgcgcctc ctccgccttc ctcctcccgt gcaggcgtgc ctgcgtgacg gaagcctgtc
    10561 ggtcggccac gcccgcgccc tgatcccggt cgaggacgag aagacccagc tccagatgct
    10621 ggctaaaatc gtggacggtg gactgtcggt acgcgcggtc gaggacctgg tcaaggcctg
    10681 gcatcgccgt cgggaccgct cggagaggga tgcgcccgag cccatgcagc cggcccggtc
    10741 cacgcgggac atgcttcagc tccgggccct cacggatcgc ctgcgctcgt cgcttggcac
    10801 gcgggtccag atccggcccg gcgcgtcggg cgagggcggc cggatcgagg tcgcgtacta
    10861 cagcgacgac gatctcgagc gcctgatcga cctgatgggg tcgtgagtat ccctcggcct
    10921 tcccgccggg cgcccgggcg ggtagtcgcg gccgcagtgg tgctcgtggc cttcgccccg
    10981 gggagcggca cgcttcgcgc ccggcccgtc gcactcccgg ccgccgccga caccacgacc
    11041 ggcgcccggg gccggtcgcc gcgcggagcg ctgctcaggt cggtcgtcgt gccgggctgg
    11101 ggccaagtct acaacgggca gcccctcaag gctccagtga tagtggcggc cctcggtgga
    11161 ctggcctacg cggtcgtgac cctcgacggg gattacaggc tgtaccgcga ggcataccag
    11221 tacaaggcct tccaggagct cgtcgaccgg ggcgagctca ccgagaatcc cagggagggc
    11281 tacgccgact cgtacgaccg ggtggccgcg gacgtcggcg ccgtgtcttc cggcccgctc
    11341 cgggcgcagc gcgatgcgct gcggcgaaac cgcgacctct cgatcctcgg catcgggctg
    11401 gtctgggcca tctcggcgct ggacgcctac gtcagcgccc acctgctcga tttcgacgtc
    11461 ggggaggacc tgacgctccg cgttcggccg gagatcgccc cgggaacggg cgtcgtgccg
    11521 gcccctgccc tcgtctggcg accggggcgg cgctgatcct ccgggcccgg ctcgcgggcc
    11581 ggcccgcgga g
//
LOCUS       DBNHQZ010000211         2019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2095276, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000211 DBNHQZ010000000
VERSION     DBNHQZ010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2095276"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..779
                     /locus_tag="AC16YU_05665"
     CDS             288..779
                     /locus_tag="AC16YU_05665"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6064744.1"
                     /translation="MDALQQLRRLWQHLLWADGLVLEALLPDAGPAGAWREYVHLLAV
                     EELWLARLEQRRMAVAVWPRLSSDEVIALREQVMTGYEAYFAGLTLEGLADSIPYTTS
                     EGDRFESVAGDILLQVILHGQYHRGKVNVLLRQAGSEPAPVDYIAFVRGAPAAVTPIE
                     PPG"
     gene            983..>2019
                     /locus_tag="AC16YU_05670"
     CDS             983..>2019
                     /locus_tag="AC16YU_05670"
                     /inference="COORDINATES: protein motif:HMM:NF028473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR6064745.1"
                     /translation="MRNIRFLSAILTLFLGIQAGPAIAQDPTIGYWDPRFEPADLGGL
                     GFQRLVEAMAVDPVSGDVFVGGGNLLRFTGEPVVRWTGSGWASVGGSFNNAVNALAFA
                     PDGTLYAGGNFTTIDGSPAGRVARLEGGTWIPLGNGVNGAVFALTVDPATGHLYVGGN
                     FTQAEQTDGTPLPTRDLVKWTGTEWERVGHGGDGLRIDALAFDPISGDLFVGGYLSPG
                     LRNTDSTLVAIQDLGRFDGDTWVDLEDPAFRLNVVNAIAFDSEGQLYVAHNIDGGLAF
                     WDRADWSMPAGGLGYIQPNGVRTGDNGTSLAVGDGRVYVGGGFTYVTQPDGSTILARG
                     VVAYDPNTDRF"
BASE COUNT          320 a          666 c          700 g          333 t
ORIGIN      
        1 ggatgccgag cccgtcaacg cggatggttt caggagacac gtgtcggcgt acagggccga
       61 agtcatcccg aacgcgggtc acctgctcat gtgggacgcg tctgcggagt tcaaccgcct
      121 gctcgaggag agcgtcgcgg agttcgacag cctggcccag gggagataag ggactgacgc
      181 ctggctgcat gcacccggac gcgcatctcg tcggcgacgg catccggaac cgggtggagg
      241 gcgtgtgacg tttcgcaccg ggcaggcgcg cacggaggga catcgacatg gacgcacttc
      301 agcagcttcg gaggctgtgg caacacctgc tgtgggcgga cgggctcgtg ctcgaggcac
      361 tcctccccga cgcgggaccc gcgggggcct ggcgggaata cgtgcacctc ctcgccgtcg
      421 aggagctctg gctcgcgagg ctcgagcagc gccgcatggc cgtagcggta tggcccaggc
      481 tgtcgagcga cgaggtgatc gcgctccggg agcaggtcat gacaggctat gaagcctatt
      541 tcgcggggct gactctcgag ggcctcgccg attcgattcc gtacacgaca tcggagggag
      601 accgattcga gagtgtggcc ggggacatcc tgctccaggt gatccttcac gggcagtacc
      661 accgcggcaa ggtgaacgtg ctgctccggc aggccggaag cgagccggcg ccggtcgact
      721 acatcgcttt cgtccgcggc gctcccgccg cggtgacacc gatcgaaccg ccgggatgag
      781 cgcacccggc tggagcggga ggcgcacgcg gcgtcggcag cgggatgggt ctcgccggat
      841 tttcggagtc gggcgtgtcc ggccgggtgc ggtttctgcg cctgtacgat tacggcttcg
      901 tccgaacagg ccgtggcaga ctgtgcacga ccgcggatat cgccgacatc cccgtcccgc
      961 gaaccgctac ccccttcgcg ccatgcggaa tatccgcttc ctttccgcca tactcacgct
     1021 cttcctgggt attcaggcgg gcccggccat cgcccaggac cccacgatcg ggtactggga
     1081 tccccgattc gaaccagccg acctcggcgg tctgggcttc cagcgcctgg tcgaggccat
     1141 ggccgtcgac ccggtgtcgg gtgacgtgtt cgtgggcggc ggcaacctgc tccggttcac
     1201 gggtgagcct gtcgtacgct ggacgggcag cggctgggca tccgtgggcg gctcgttcaa
     1261 caatgccgtc aatgccctgg cgttcgcgcc cgacggcact ctgtacgccg gcggcaattt
     1321 caccacgatc gacggcagtc cggcgggccg agtcgcacgc ctcgaaggtg gaacgtggat
     1381 cccgctcggc aacggcgtaa acggagctgt ctttgcgctc acggtggatc ccgccaccgg
     1441 acacctctat gtcggcggca acttcaccca ggccgagcag accgacggca ccccactgcc
     1501 gacccgggac ctcgtgaaat ggaccggcac ggagtgggag cgcgtgggtc acgggggcga
     1561 cgggcttcgg atcgacgcgc tcgccttcga cccgatttcc ggggacctgt tcgttggcgg
     1621 atacctcagt cccggactcc gcaacaccga cagcacgctg gtcgccatac aggacctggg
     1681 gcggttcgac ggtgacacct gggtcgacct cgaggacccg gcgttcaggc tcaacgtcgt
     1741 gaatgccatc gcgttcgatt ccgagggtca actctacgtg gcccacaaca tcgacggcgg
     1801 gttggccttc tgggatcgcg ccgactggtc catgccggcg ggtgggctcg ggtacatcca
     1861 gccgaacggc gtgcgcacgg gcgacaacgg cacctccctt gccgtgggtg acggacgcgt
     1921 atacgtcggg ggcggattca cctacgtgac ccagcccgac ggctccacga tcctggcgcg
     1981 cggcgtcgtg gcctacgatc cgaataccga ccgtttcga
//
LOCUS       DBNHQZ010000212         2514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1824448, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000212 DBNHQZ010000000
VERSION     DBNHQZ010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1824448"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16YU_05675"
     CDS             complement(<1..242)
                     /locus_tag="AC16YU_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064746.1"
                     /translation="MTTYRNVALAATLLLCTLPASAQTAHRPVGLPPAPTVVVRAGHV
                     IDVANGRAIPDQSILIQDGRIAAVGDRVVAPDGTPV"
     gene            499..936
                     /locus_tag="AC16YU_05680"
     CDS             499..936
                     /locus_tag="AC16YU_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR6064747.1"
                     /translation="MTSATNQDARTWTVLFLCWLLASVATLGSLFFSEVMELPPCSLC
                     WYQRIFLFPLVVLFTAGLYPADGQVVRFALPLVVGGWLVAGYQNLLVWGVVSESVVPC
                     SLGVSCSEVDFELFGFVTIPLLSWLAFTTLGGLLLYLKRKPVP"
     gene            933..1592
                     /locus_tag="AC16YU_05685"
     CDS             933..1592
                     /locus_tag="AC16YU_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6064748.1"
                     /translation="MKKNHLVVPVGALLIVAFVIAAFVFRSNRTEELAFMARENAELF
                     VRDHSRVIGPADAKVHLIEFTDPACETCAAFHPFTRHLMQAHAGKVKVVVRYAPFHAG
                     ADQVVRVIEAAARQADYERVLDLVLRTQESWAINHQARPELLIPILGQAGLDVERLRA
                     DMDDPEITRIIQQDLADAQALGVQKTPGFFANGKPLVSFGYEQLQALVEAEVRAAYPN
                     G"
     gene            complement(1675..2139)
                     /locus_tag="AC16YU_05690"
     CDS             complement(1675..2139)
                     /locus_tag="AC16YU_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6064749.1"
                     /translation="MRTTLLVLFLLLVGVQGSRARQNPVSPSAAGLSAQEQELLDLSR
                     AKWIWMAERKADTLAALFHDEAVFVHMGGTMSKDQELGVIRTGGIHYKQADIHGASVR
                     TIGDTGIVLNTITLLAVVGGNEVTNPFEVTEVYVREDESWKLAVLAFTRLSR"
     gene            complement(2306..>2514)
                     /locus_tag="AC16YU_05695"
     CDS             complement(2306..>2514)
                     /locus_tag="AC16YU_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR6064750.1"
                     /translation="MAKIRSALGDRFEVFTSLDGAAHDAAVEMAAAAESMDLTAVLDA
                     QVRARNACIACHGSFREDVRRALY"
BASE COUNT          383 a          841 c          859 g          431 t
ORIGIN      
        1 atgaccggcg tgccgtcggg cgccacgacg cgatcgccga cggcagcgat cctcccgtcc
       61 tggatcagga tggactggtc ggggatcgcg cggccgttcg caacgtctat gacgtgcccc
      121 gcccggacga cgacggtcgg tgccggcgga aggccgacgg ggcgatgggc ggtctgggcg
      181 gatgcgggca gcgtgcagag gagaagcgtc gccgcgaggg cgacgttccg atacgtcgtc
      241 atggttggtc cgggtgggtg aaccgtaacg gtaactctcc cgccgggacc ctcgcaaatg
      301 tccggtcggc gaacgccggc gtgaggcatg cgacgtacgg ggacgggttg gcggccgggg
      361 ctcgtcgtcc tgccggcggt cccgtactct gctcccgaac gtcggggaat ccccgtcggc
      421 accgcatcct ttggcggatt atccttgccg cccgatcccc agaacgaggc ctccgcgtac
      481 cggcggcgaa ccgatcccat gacgagcgcg acgaaccaag acgccaggac gtggaccgtc
      541 ctcttcctgt gctggcttct ggccagcgtg gcgacgctgg gaagcctgtt cttcagcgag
      601 gtcatggagc ttcctccctg ctcgctgtgc tggtaccagc ggatcttcct gtttccgctg
      661 gtcgtcctgt tcaccgccgg cctgtacccg gcggacgggc aggtggtccg gttcgcgctc
      721 ccgctcgtcg tgggcgggtg gctcgtggcc ggttaccaga acctgctggt ctggggcgtc
      781 gtatccgaga gcgtcgtgcc ctgcagcctg ggcgtctcct gttccgaggt cgacttcgag
      841 ctgttcggat tcgtgacgat tccgctgctt tcctggctcg ccttcacgac gctcggcggc
      901 ctgctcctgt acctgaagcg aaaaccagtt ccatgaagaa gaaccacctc gtcgttcccg
      961 taggcgccct gctgatcgtg gcattcgtca tcgcggcctt cgtcttccgc tcgaatcgca
     1021 cggaggagct cgcgttcatg gcgcgggaaa acgccgagct cttcgtgcgt gaccattccc
     1081 gcgtgatcgg tccggccgac gcgaaggtcc acctgatcga attcaccgat ccggcgtgcg
     1141 agacgtgcgc tgccttccat ccgttcacgc ggcacctcat gcaggcccac gcgggcaagg
     1201 tcaaggtcgt cgtgcgttat gcgccgttcc atgccggggc cgaccaggtc gtacgcgtca
     1261 tcgaggcggc ggcgcgccag gccgactacg aacgggtcct ggacctggtg ctgcgcacgc
     1321 aggaatcgtg ggctatcaat caccaggccc ggccggagct gctcattccg atcctggggc
     1381 aggcgggcct ggacgtcgag cggctccgcg cggacatgga cgaccccgag atcacgcgca
     1441 tcatccagca ggacctcgcc gacgcccagg ccctcggcgt ccagaagacg ccgggctttt
     1501 tcgcgaacgg caagccgctg gtgagcttcg gctacgagca gctgcaggcg ctggtggaag
     1561 ccgaggtccg ggcggcatac ccgaacggat gacccgctcc gaaccggacg cggcccgcac
     1621 ccgtcagcga tcgatgtagc gctgggagct ctcggaatac cgcgcccctg tggactacct
     1681 cgacagcctc gtgaacgcca ggacggccag cttccaggat tcgtcctccc gcacgtagac
     1741 ctccgtgacc tcgaacggat tggtcacctc gtttccgcct accacggcca gtagggtgat
     1801 cgtgttcagg acgatgcccg tgtcgccgat ggtccggacc gacgcgccat ggatgtccgc
     1861 ctgcttgtag tgaatcccgc cggtccggat cacgccgagc tcctggtctt tcgacatggt
     1921 cccgcccatg tggacgaaga cggcctcgtc gtgaaacagc gcagccaggg tgtccgcctt
     1981 gcgttcggcc atccagatcc acttggctct cgagaggtcc aggagctcct gctcctgggc
     2041 cgagaggccc gccgcggagg gcgagaccgg gttctgccgt gcgcgggagc cctgcacgcc
     2101 gacgagcagc aggaacagga cgagaagggt cgttctcatg ggaggattcg gggttgcggc
     2161 cctgcggtgg agcagagcgg tgtggcacgt ggaagtcacg gcaccatgat agacccggcg
     2221 gggcaggtca gagatccgga acgttcctca aggagatagc tgcggcgctc ccgaagcgca
     2281 caccccgcag ccgcccgccg acggctcagt acagcgccct gcgcacgtcc tcgcggaagg
     2341 agccgtggca cgcgatgcag gcgttccggg cgcgtacctg ggcgtcgagc acggcggtca
     2401 ggtccatgga ctcggcggcg gccgccatct cgacggcggc gtcgtgcgcg gccccgtcca
     2461 gcgacgtgaa gacctcgaag cggtctccga gggccgagcg gattttcgcc atct
//
LOCUS       DBNHQZ010000213         5786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1285671, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000213 DBNHQZ010000000
VERSION     DBNHQZ010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1285671"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3347
                     /locus_tag="AC16YU_05700"
     CDS             <1..3347
                     /locus_tag="AC16YU_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064751.1"
                     /translation="TATLNAIAFASTAPVDFAAATIVHVDDEVSVTDDLDIDEVIDET
                     YGPFTDDATIPYSRDFGCSADPADYTDGAYSYVKENRAAIVETLDSDDASVTVECYMP
                     SVSKNAFSSLTRTYEWSIVKSVTPEAHQLFAGGSANSDYTVTVTNTGSVDSDWLVFGS
                     ISVTNPHPTADLVVALEDALDDGTVATLECENPLTIAAGATVDCAYEAVPADGSATEN
                     TVTFLLNEIEFSDTFAFDFEEGTITELFGEVNVTDAFDGADPVSLGDPVLAGGPPAVF
                     EYPRTFECSSDIADYTEGMYGDAFADTATIVETEQSDDANVSLECYAPLVSKTAETSR
                     VRTYDWTIDKLVDPEAHALFDGDTAESQYTLTVSKELLSEEYGVMGVVTIHNPAPMDL
                     LVAVQDMLGGEVVDLGACDDDNAGILAGETLECPYSTAVPTNEQTTNEVSVTIVATEA
                     VFTASADVVFNGGTDVSELNPEVFVDDTSDFAADANGEAAGKWTFSSDIELDSESWPV
                     QYLGTFDCADVEYTDGHGSYEVPNTATIIGGDSDDALVTVDCYELAIDKTASSSFTRD
                     WTWEIAKSADAGGADSDILNRPEAVMLAEGQSYLGVAYVVSATASSLDSDFLVTGEIT
                     IDNPHPTRAAELDGVTDLLTGDLAAAVDCPAMEVPAGGSLVCAYEADLPDGEARTNTA
                     TALNRNYHFGFAADPVVLGSTPYVATLAVAFDLEVPTVETDECADVGDSWFGDDPLTA
                     VVETALGTICAADLMASDPFLFPYGMNIIPEGVSVPDTGTPFVTPECGENTFPNWASL
                     ITNDNAETAQAPWFVDVNVVCDFGCTLTQGYWKTHSEYGPAPYDETWALLESGADTEL
                     FGSGMSWHEVFWTAPAGNAWYNLAHQWMAASLNVLNEASTPDEVEAALADGEALLAMG
                     PEAFIAPKGGAKKTPEGQAAVELIAQAREVAGLLAMYNEGYIGPGHCDEDDLSEPETY
                     TNAGMVSISSDDLSEVTEAEADVKAGSTEDAMLDTESGINEASAADAGPAQAIELPTE
                     FAVGNYPNPFNPTTTIQVALPEASRVRVSVYDLLGRMVSLLVDGELSAGTHSVTFEAG
                     SLPSGVYLYRLEYSGGAVTRTMQLLK"
     gene            3787..>5786
                     /locus_tag="AC16YU_05705"
     CDS             3787..>5786
                     /locus_tag="AC16YU_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064752.1"
                     /translation="MYKPYSLLLLFLAYALLPAAGQAQPEILTPDPSGPDIVIQDDGV
                     YSYRGVVFQQGDPVNGGTGNLHPFLRIAGNTPMIHGVNTANADEQFDAASTWTNGLLL
                     STITQRIVDVDGTEILFREFVLDINQSANRPWYPLNDIQIYENQANVDDMNCLDPLAF
                     GEAIDQRWDETGAWCGGTPYVTGTATLEFWLDQEANATAYLDYDWEAGSGEPDLVMLV
                     PDSYFDQDPTCDYGQSGCETFVYVYAMFGDVADAPLCTQSGGIEDCEGNTANFEEWAT
                     FVYPFVQVSKTAVASVDQTYTWDIDKVVVPTDCDALAGDDCSFEYEVTIDQTVEFGSP
                     SLTGVITITNPRNFEYSGATTSAAVVDYLVDVFPTATSVSITGCTVPFTVNSGQTVNC
                     PYTAVFPPGYEPVDGTNTATVGLEEISAFFEATAPVVFTVNQIGYEYVEVTDDLQGLL
                     GNVSGDDSFTYPFLYTCSSDPGAYTDGHYQYTRHNTATIVETAQSDDANLTVDCWAPV
                     VSKTATATYDRTHSWTIDKVADPVEHHLFMGEMEQSAYDVAVDKTTIEDNFRVTGGIS
                     IHNPSPLVMDLTVSDELDTGLPANLDCGPAVSINPGGTQDCEYNATPPNASATSNTVT
                     VELESGATFAATVPFAFTVNVIGHDAIDVDDTVLGDGIFAAT"
BASE COUNT         1037 a         1893 c         1943 g          913 t
ORIGIN      
        1 cgacggcgac gctcaacgcc atcgcgttcg cgtcgaccgc tccggtcgat ttcgcggcgg
       61 cgacgatcgt gcacgtcgat gacgaagtga gcgtcaccga cgacctggac atcgacgaag
      121 tgatcgacga gacctacggt ccgttcacgg acgacgcgac gattccgtat tcgcgcgact
      181 tcggctgttc ggccgacccc gccgactaca cggatggtgc gtactcctac gtgaaggaga
      241 accgcgccgc gatcgtcgag acgctcgatt ccgacgatgc gagcgtcacg gtcgagtgct
      301 acatgccctc ggtctcgaag aacgcgttct cgagcctgac ccggacgtac gagtggtcga
      361 tcgtgaagag cgtgacgccc gaggcgcacc agctcttcgc cggcggctcg gcgaattcgg
      421 actacacggt gaccgtgacg aacaccgggt cggtcgacag cgactggctg gtcttcggct
      481 cgatctccgt cacgaacccg cacccgacgg cggacctggt ggtcgcgctc gaagatgcgc
      541 tcgatgacgg gaccgtggcg acgctggaat gcgagaatcc gctgaccatc gcggccggtg
      601 cgacggtgga ctgcgcgtac gaggccgttc ccgcggacgg ctcggccacg gagaacaccg
      661 tgacgttcct gctgaacgag atcgagttca gcgatacgtt cgcgttcgac ttcgaggagg
      721 gcacgatcac cgagctcttc ggcgaagtga acgtgaccga tgcgttcgac ggcgcggatc
      781 ccgtgtcgct cggcgatccg gtcctggcag gcggcccgcc cgcggtgttc gaatacccgc
      841 ggacgttcga gtgctcgtcg gacatcgccg actacacgga agggatgtac ggcgacgcgt
      901 tcgccgacac ggccacgatc gtcgagacgg agcagtccga cgacgcgaac gtgagcctgg
      961 agtgctacgc gccgctggtg tcgaagacgg ccgagacgag ccgcgtgagg acgtacgact
     1021 ggacgatcga caagctggtc gatccggaag cgcacgccct cttcgacggt gacacggccg
     1081 agtcgcagta cacgctgacc gtgagcaagg aactcctgag tgaggaatac ggcgtcatgg
     1141 gcgtcgtcac catccacaac ccggcgccga tggacctcct ggtcgcggtg caggacatgc
     1201 tgggcggcga ggtcgtggac ctcggcgcgt gtgacgacga caacgccggc attctcgcag
     1261 gggagaccct cgagtgtccg tactcgacgg cagtgccgac gaacgaacag acgacgaacg
     1321 aagtgtccgt gaccatcgtg gccacagagg ccgtgttcac ggccagtgcg gacgtcgtct
     1381 tcaacggcgg gacggacgtg tcggagctga atccggaagt gttcgtggat gacacgagcg
     1441 acttcgcggc cgatgcgaac ggcgaggctg cgggcaagtg gacgttctcg tccgacatcg
     1501 agctcgacag cgagtcgtgg ccggtccagt acctgggcac gttcgactgt gccgacgtgg
     1561 agtacacgga cggtcacgga tcgtacgagg tcccgaacac ggcgacgatc atcggcggcg
     1621 attcggatga cgcgctcgtc acggtcgact gctacgagct cgccatcgac aagacggctt
     1681 ccagctcgtt cacccgtgac tggacgtggg agattgccaa gtcggctgac gccggcgggg
     1741 cggattccga catcctgaac cgtcctgagg cggtcatgct ggcggagggg cagtcgtacc
     1801 tgggtgttgc gtacgtcgtt tcggcgaccg catcgtccct ggacagcgac ttcctcgtga
     1861 cgggcgagat caccatcgac aacccgcacc cgacccgggc ggcggagctc gatggcgtga
     1921 ccgacctgtt gaccggtgac cttgctgccg cggtggattg ccccgccatg gaggtgcctg
     1981 ccggcggttc gctggtctgc gcctacgagg cggacctgcc cgacggcgag gcccgcacga
     2041 acacggcgac ggcgctgaac cggaactacc acttcggctt cgcggccgat ccggtggtcc
     2101 tcggcagcac gccgtacgtg gccaccctgg cggtcgcgtt cgatctcgag gttccgaccg
     2161 tcgagacgga cgagtgcgcc gacgtgggtg actcctggtt cggagacgat ccgctgacgg
     2221 cagtggtcga aaccgctctc ggcacgatct gcgcggccga tctcatggcg tccgatccgt
     2281 tcctcttccc gtacgggatg aacatcatcc ccgagggagt gtcggttccg gataccggga
     2341 cgccgttcgt cacgcccgag tgcggtgaga acacgttccc gaactgggcg agcctgatca
     2401 cgaacgacaa cgcagaaacg gcccaggctc cctggttcgt ggacgtaaac gtcgtttgcg
     2461 acttcggctg caccctgacg cagggctact ggaagaccca ctccgagtac ggcccggcgc
     2521 cgtatgacga aacctgggcc ctactcgagt ccggggcgga cacggagctc ttcggctccg
     2581 ggatgtcctg gcacgaggtc ttctggacgg ccccggccgg aaacgcctgg tacaacctgg
     2641 cgcaccagtg gatggcggca tccctgaacg tcctgaacga ggcgagcacg ccggacgaag
     2701 tggaagcggc actcgccgac ggcgaagcgc tgctggccat gggtccggag gccttcatcg
     2761 cgccgaaggg cggtgcgaag aagacgccgg aaggacaggc ggccgtggag ctcatcgccc
     2821 aggcccgtga ggtcgcagga ctcctcgcga tgtacaacga gggctacatc ggccccggcc
     2881 attgcgacga ggacgacctc tccgagcccg agacctatac caatgccggt atggtctcga
     2941 tctcgagcga tgacctgagc gaggtgaccg aggccgaagc cgacgtcaag gccggttcga
     3001 ccgaggatgc catgctcgac acggagagcg gaatcaacga ggccagcgcc gccgatgcgg
     3061 ggccggccca ggcgatcgag ctcccgaccg agttcgcggt gggcaactac ccgaacccgt
     3121 tcaacccgac caccacgatc caggtggccc tccccgaggc ctcgcgcgtg cgagtctcgg
     3181 tttatgacct gctgggtcgg atggtatcgc tgctcgtgga tggcgaactc tcggcgggca
     3241 cgcacagcgt gaccttcgag gcgggctctc tgccgagtgg cgtgtacctg taccgtctcg
     3301 agtactcggg cggtgcagtc acccggacga tgcagctgct caagtagccg catcccttcg
     3361 ggacgagagg cacgggccgg gccgccgggg gggcggcccg gccctgtgcg tttgaggggt
     3421 ctcgacgttc gaggcggccc ggtgtgcgca ttcggcgcgg tccggcagcg aagtttcgcc
     3481 gcgaaggccg gagcgttcgg agataggggc gccgatcggt gcctacgccg cgtcgcaggc
     3541 tcgtccggca ttctgtgacc gccgcctcgc ataagatagc attccataca acggtccggc
     3601 cgggctcccc caacgagccg cccggccgag cggcgggacc cctccacctg gcatccctcc
     3661 ggccatggct gcccaccgag tcgcttcgcg cgaggcgcag ccggtccagg accggcgccc
     3721 gcgatcggcc ccgtcgcgtt ttcgcgcacc gaaccaccgg ataagggagg tacccttatg
     3781 aatcccatgt acaagcccta ttccctgctc ctgctgttcc tggcttatgc gctgcttccg
     3841 gcggcgggcc aggcgcagcc ggagattctg acgcccgacc cgtcgggccc ggacatcgtg
     3901 atccaggatg acggtgtcta cagctaccgc ggagtcgtct tccagcaggg cgacccggtg
     3961 aacggcggca cgggcaacct gcacccgttc ctgcgcatcg cgggcaacac gcccatgatc
     4021 cacggcgtga acacggcgaa cgcggacgag cagttcgacg ccgcgtccac gtggacgaac
     4081 ggactgctgc tcagcaccat aacccagcgc atcgtggacg tcgacggcac ggagatcctg
     4141 ttccgcgagt tcgtgctcga catcaaccag tcggcgaacc gtccctggta cccgctgaac
     4201 gacatccaga tatacgagaa ccaggcgaac gtggacgaca tgaactgcct ggatccgctg
     4261 gccttcggtg aggcgatcga ccagcgctgg gacgagaccg gcgcgtggtg cggcggaacg
     4321 ccctacgtga ccggcaccgc cacgctcgag ttctggctgg accaggaggc gaacgcgacc
     4381 gcgtacctgg actacgactg ggaggccggc agcggcgagc ccgatctcgt gatgctggtt
     4441 ccggactcgt acttcgacca ggacccgacc tgcgactacg ggcagtcggg atgcgagacg
     4501 ttcgtgtacg tgtacgcgat gttcggcgac gtggccgacg caccgctctg cacgcagtcg
     4561 ggcggcatcg aggactgcga gggcaacacg gcgaacttcg aggagtgggc gaccttcgtg
     4621 tacccgttcg tgcaggtttc caagacggcc gtcgcgtcgg tggaccagac ctacacctgg
     4681 gacatcgaca aggtggtcgt tccgaccgac tgcgacgcgc tcgcaggcga cgactgcagc
     4741 ttcgagtacg aggtgacgat cgaccagacc gtcgaattcg gcagcccgtc gctcacggga
     4801 gtcatcacga tcacgaatcc acgcaacttc gaatactcgg gcgccacgac gtcggcggcc
     4861 gtggtggact acctcgtgga tgtgtttccg acggcgacct cggtcagcat cacgggatgc
     4921 accgtgccct tcacggtgaa ctccggccag acggtgaact gtccgtacac ggccgtcttc
     4981 ccgcccggct acgagccggt cgacggcacg aacacggcaa cggtcggcct cgaggagatt
     5041 tccgccttct tcgaggcgac cgcccccgtc gtgttcacgg tcaaccagat cggctacgag
     5101 tacgtcgagg tgacggacga cctccagggc ctgctgggca acgtgtcggg tgacgactcc
     5161 ttcacgtatc cgttcctgta cacgtgctcg agcgatccgg gcgcgtacac ggacgggcat
     5221 taccagtaca cgcgccacaa cacggcgacc atcgtcgaga ccgcgcagtc cgacgatgcg
     5281 aacctgacgg tggactgctg ggcgccggtc gtctcgaaga cggccaccgc cacgtacgat
     5341 cgtacgcact cgtggacgat cgacaaggtg gccgatcccg tcgagcacca cctgttcatg
     5401 ggcgagatgg agcagtccgc ctacgacgtg gcggtcgaca agaccacgat cgaggacaac
     5461 ttccgcgtga cgggcgggat ctcgatccac aacccgtcgc cgctcgtgat ggacctcacc
     5521 gtctcggacg agctcgacac cggcctcccg gccaacctgg actgcgggcc ggcggtgagc
     5581 atcaacccgg gagggacgca ggactgcgag tacaacgcga ctccgccgaa cgcctcggcc
     5641 acgagcaaca cggtgacggt cgagctggaa agcggtgcga cgttcgcggc gacggtgccc
     5701 ttcgccttca cggtgaacgt gatcggccac gacgcgatcg acgtggacga caccgtcctg
     5761 ggcgatggca tcttcgcggc gacgga
//
LOCUS       DBNHQZ010000214         8106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_336242, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000214 DBNHQZ010000000
VERSION     DBNHQZ010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_336242"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16YU_05710"
     CDS             <1..971
                     /locus_tag="AC16YU_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064753.1"
                     /translation="TGADIGLSVISSYVAPIVLQLGTNMVVNADLEADYYQDARIHPD
                     GSRAVLGVSEPGESEVRQDIWLLDFETGARRRLTETGDASFPVWTAGGDSLLHFRFPG
                     GGQNTFVVTRAADGSGRERTVHRGEAPGKWDLDVARDGRMIAYVEGVPSLEDRITTLS
                     VYRLPGNEEVADFLGRFDNPRRPAFSPDGRYLAFEEQGAIRVISLADPEAVPLTVWAR
                     GMVQPRWAPDGTALLATAADGNPHTVDITLDPVFAVRSTSRQAANWPVAGSNLFDVFP
                     DGRTVIVPGLPDDAAGGPSSPADAQSGTFDLHFVIGWFDELRRQSR"
     gene            1080..2345
                     /locus_tag="AC16YU_05715"
     CDS             1080..2345
                     /locus_tag="AC16YU_05715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6064754.1"
                     /translation="MMRRFVPYLALVLLLAPARAQDFESVPPEEVGFSTERLGRLTAV
                     MQEYVDDGLLAGAVVLVLRDGYAAYEGTFGYLDREAGTPMPPDAIFRIASQSKAPVSV
                     GIMMLQEEGKLLIGDRLGKYLPEFSETTVAVAREGGGFDVVPAERPITLRDLLTHTAG
                     IGYGYGPAAAEWEAAGVQGWYFAHRDEPIRETVRRMAGLPMDAQPGSGFVYGYNTDIL
                     GAVIEVVSGQPLDRFLSERIFEPLDMTDTHFYLPPEKAGRLAVVYSATDQGLVRAPDE
                     SAMQAQGAYVEGPRASFSGGAGLLSTARDYARFLQMLLNGGELDGVRLLSRTTVSLMI
                     SDHLSGLPYPWAPGNGFGLGFRILKDPGTFGRPGSVGAFEWGGAYHSKYWVDPAEGLV
                     VVYFTQLIPTGGVDDLEKLWALVYQAIVD"
     gene            2603..2687
                     /locus_tag="AC16YU_05720"
     tRNA            2603..2687
                     /locus_tag="AC16YU_05720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2637..2639,aa:Leu,seq:cag)
     gene            2808..4850
                     /locus_tag="AC16YU_05725"
     CDS             2808..4850
                     /locus_tag="AC16YU_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064755.1"
                     /translation="MPSAIHTNRTARVVRGLAPGLATLLSAGVLFAACGRSAEAPADP
                     DAYLNWGPDARYVGKAVCGECHRENHGTFVASQMGRSFRPARDSLSSADWTQDDPVYD
                     PGRDLWYQPVRRGQLLFVREFRLSGRDTVHNRLERIHWIVGSGQHTNSHMREINGYLY
                     QIPLTWYAQAKKWDLPPGFDGGDSPRFNRPIPDLCMSCHDGPSGYVPESENRFDYIPH
                     GIECENCHGPGSVHEEAMRAGRVVNTDKEIDRTIVHPGKLPPALQYDICRKCHMQGAM
                     VLRDGRSPFDFRPGKRLTDYMDTFWPRFPDSARSFIMASHPDRLQMSACFRASWDESR
                     GLPEMTCVTCHNPHLAVELKPSFEYNAPCRACHARPDLPDCTASEEHRLEVQDDCSSC
                     HLPEAWTIDIPNVRITDHYIRVPEADRDPTPDYAQQTNLVRMASLLRDSVDLPTLARG
                     YLAYYEEFARRPFLLDSAAAVIERAPEGDSLRERVLVRVLYLREEWDRLRRLAGGRDP
                     AAEHDAWTAYRIGEAFMKAEDYPSALRWFDRVADLAPDHLRFMDRLGFALMNAGRPED
                     AVAVLDGVLERDPTLARSRNNRGFARLVIGELEAGEDDLREAIRLDPDFAEAVGNLAS
                     LLLNTGRAREAMPLAQRLVTMDPADDRYRAFLRQVTVAAQAEPVTPEARELPSPTN"
     gene            complement(4917..5432)
                     /locus_tag="AC16YU_05730"
     CDS             complement(4917..5432)
                     /locus_tag="AC16YU_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064756.1"
                     /translation="MRTSVLLMGLAMVLGGCDWTTVDERSNPLDQTTWSATFVVHNDL
                     FGADEQWSTVVNLQELDDDLAGTAEWVYQWIDPDPWLEGDPVYGLSEFVEGQKYSGDS
                     LRFALYTAPASEDYDCDLLDRASGCTSVRPAALYFFGALTSDSTMAGYGVTYTGTTYA
                     RVDDLVFWRAD"
     gene            5635..6687
                     /locus_tag="AC16YU_05735"
     CDS             5635..6687
                     /locus_tag="AC16YU_05735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6064757.1"
                     /translation="MSILVYGAYGYTGRLIVEEALRRGLEPVIAGRNASRLRKLRMRT
                     GLETRTCSLDDEAGLLAMLRGVRVVVHAAGPFTVTSKPMGRACLAAGVHYLDITGEIP
                     VFEKMAALDGRARERGILLMPGVGFDVVPSDCLAAHLKRRLPTATRLSLAIAREGSGM
                     SRGSLRTVLRHLGGGSIERVDGNLVPAPLGDKRMEVDFGSGPRRVISAPWGDLGSAYH
                     STGVPNITTYWAYPPLMAQAARRLHWFRPLLRTRAAQILLHRAAGLFHSEGPDARARE
                     TGRSLVWGEMRDDEGHSVRARFRGPEIYALTARLAADIALRVRDGEGRPGFATPSMLF
                     GPDYILGFGGVVREDL"
     gene            6769..>8106
                     /locus_tag="AC16YU_05740"
     CDS             6769..>8106
                     /locus_tag="AC16YU_05740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR6064758.1"
                     /translation="MFRALILALIVTISASLDASARQTTGFYRVEGKKVVDANGQPAM
                     RRGIGLGGWLMPEGYMLHFPGHGSATNMRSKVRDLIGDSDAAEFWRLYRENYVDEKDI
                     AALAAAGFDHVRLPIHYDVVYDLASRTFKPEGYELLDTFIDWCRTHGLGVILDLHAAP
                     GAQNEGEISDSDGQARLWTQPNPYQGITAAIWGETARRYSNETQIIGYDLLNEPVVTT
                     DMVTDPPAALRALYELLTTAVRSEDPNHILFIEGNYWATTFDNLTPPFDDKLVYAFHK
                     YWNATDIGTIRYLLAIRDTYDVPLWLGESGENSNAWFRAVVDLMEEEAIGWNWWTHKK
                     IQSTTSPWSALIKPGYRTVLDYWAGSGGRPSVQAAREGLFEMARGLDLDSTRFDPDVL
                     AALFDPTFGETGHPYADHVIPGVVNAADYDTGLPGTTYSDRDNWAVSGSPGGGN"
BASE COUNT         1259 a         2754 c         2800 g         1293 t
ORIGIN      
        1 acaccggggc tgacatcggg ctgagcgtga tctccagtta tgtggctcct atcgtgctcc
       61 agctcggcac gaacatggtc gtgaacgcgg atctggaggc ggactattac caggacgccc
      121 ggattcatcc ggacgggtcg cgagccgtcc tcggcgtctc ggagcccggg gagtcggagg
      181 tccggcagga catctggctg ctggatttcg agaccggagc ccggcgccgc ctcaccgaga
      241 ccggtgacgc ctcgttcccg gtctggaccg cggggggcga cagcctgctg cattttcgct
      301 ttccgggcgg cggacagaac acctttgtcg tgacgcgggc ggccgacggc tcgggccgtg
      361 agcgcacggt gcaccgaggc gaggcgcccg gcaagtggga cctggacgtc gcgcgggacg
      421 ggcggatgat cgcctacgtg gaaggcgtac ccagtctgga ggaccgcatc acgacgctat
      481 cggtctaccg cctgccgggg aacgaggagg tggccgactt cctgggccgg ttcgacaacc
      541 cgcgccggcc ggcattctcg ccggacggcc ggtacctggc cttcgaggaa cagggggcca
      601 tccgcgtgat ttcgctcgcc gatccggagg ccgtcccgct cacggtgtgg gcacggggca
      661 tggtgcagcc ccgctgggct cctgacggga cggcactgct ggcgaccgcg gcggacggga
      721 atccgcacac cgtcgacatc acgctggatc ccgtctttgc ggtccggtcc acttcccggc
      781 aggccgccaa ttggcccgtc gcgggatcga acctcttcga cgtgtttccg gacgggcgga
      841 ccgtgatcgt cccgggcctt ccggacgatg cggccggcgg gccgtcttcc cccgcggacg
      901 cgcagtccgg gacgttcgac ctgcacttcg tcataggctg gttcgacgag ctcaggaggc
      961 agtcgcggta gggcgaactg ccttgccgcg atgatgcgca agccgagggg tttcaccgct
     1021 tgacccgccc cgcgcggcgg cataacctcg aatctccgtc ctccgcccgg tgacccgtca
     1081 tgatgcgccg tttcgtcccg tatctagccc tcgtccttct ccttgcgccg gcccgcgcgc
     1141 aggacttcga gagtgttcct cccgaggagg tggggttctc gacggagcgg ctcgggcggc
     1201 tgaccgccgt catgcaggag tacgtggacg acgggctgct ggccggtgcc gtcgtgctcg
     1261 tgctgcgcga cggctacgcg gcatacgagg gcacgttcgg gtacctggac agggaggcgg
     1321 ggacgccgat gccgcccgac gccatattcc gcattgcttc ccagtccaag gcacccgtga
     1381 gcgtcggcat catgatgctg caggaggagg ggaagctgct catcggcgat cggctcggga
     1441 agtacctgcc ggagttttcc gaaacgaccg ttgccgtggc ccgggagggt ggcggcttcg
     1501 acgtggtacc cgccgagcgg cccatcacgc tccgcgacct gctgacccac acggcgggga
     1561 tcggctacgg ttacggcccc gcggccgccg agtgggaggc ggcgggcgtc cagggctggt
     1621 acttcgccca cagggatgaa ccgatccggg agaccgtccg gcggatggcg ggcctgccga
     1681 tggacgccca gcccggctcg ggattcgtct acggctacaa cacggacatc ctgggcgccg
     1741 tgatcgaggt cgtgtcgggg cagccgctcg accggttcct ctcggagcgc atcttcgagc
     1801 cgctggacat gacggacacg cacttctacc tgccgcccga aaaggccggc cgcctggccg
     1861 tcgtctactc cgcgacggac caggggctgg ttcgcgcgcc cgacgagagc gcgatgcagg
     1921 cgcagggcgc gtacgtcgag ggtccccggg cgagcttctc cgggggcgcg ggcctgctgt
     1981 ccaccgcccg cgactatgcc cgcttcctgc agatgctcct gaacggcggc gagctcgacg
     2041 gggtgcgcct gctctcgcgc accaccgtgt ctctcatgat ctccgaccac ctgtccggac
     2101 tcccgtaccc gtgggcgccc ggcaacgggt tcggacttgg cttccgcatc ctgaaggacc
     2161 ccgggacgtt cggccggccg ggctcggtcg gcgccttcga gtggggaggg gcgtaccact
     2221 cgaagtactg ggtcgacccg gccgagggcc tcgtcgtcgt gtacttcacc cagctgatcc
     2281 cgacgggcgg cgtggacgac ctcgagaagc tctgggcgct cgtgtaccag gcgatcgtgg
     2341 actgacgggc gtcgttccgc ggtcgggtca ggcggcctcc acagggaaac cgacgactcc
     2401 ccgggaggta ccgacgtgga agcgggaggg caggccgtga tcgtggtgga cacggacgtc
     2461 gtcgcctatg ccttcatccg aagcgatccg gtccgcacgg agcgtgacgc ttgaaattcc
     2521 ggcccatccg cgtcgggaac ttgccccgga ctccggcgat tcctaccatc tgcgcccgca
     2581 tcgcgcttcg gcgcggcatc atgccctggt ggcggaatag gtagacgcgc taggttcagg
     2641 acctagtgtc cgtacaggac gtggaggttc gagtcctctc cagggcactt gggcttcggc
     2701 ccggacccgc tctcccgcat gggggagcgg gtttctttgt ggtaacgggc aacgggtcgc
     2761 ctgggccggc accgcgcccg aatccgaccc cggccgtata atcggccatg ccgtccgcta
     2821 tccacacgaa ccgcaccgca cgcgtcgtcc ggggcctcgc gccgggcctc gccacgctgc
     2881 tctccgccgg agtgctcttc gccgcctgcg gccggagcgc cgaggcgccg gccgaccccg
     2941 acgcgtacct gaactgggga ccggacgcgc ggtacgtggg gaaggccgtc tgcggcgagt
     3001 gccaccgcga gaaccacggg acgttcgtcg cgtcgcagat gggccgctcc ttccggccgg
     3061 cgcgcgactc gctctcgagc gcggactgga cgcaggacga tccggtgtat gacccggggc
     3121 gcgacctctg gtaccagccc gtgcggcggg ggcagctgct cttcgtgcgc gagttccgcc
     3181 tctcgggccg ggacaccgtg cacaacaggc tggagcggat ccactggatc gtcggctcgg
     3241 ggcagcacac gaactcccac atgcgggaga tcaacgggta cctgtaccag atcccgctca
     3301 cctggtatgc ccaggcgaag aaatgggacc tgccgccggg attcgacggg ggggactcgc
     3361 cccgcttcaa ccggccgatc ccggacttgt gcatgtcgtg ccacgacggc ccttccggct
     3421 acgtcccgga gtccgagaac cggttcgact acataccgca cggcatcgag tgcgaaaact
     3481 gccacggtcc cgggtcggtg cacgaggagg cgatgcgggc gggccgcgtc gtgaataccg
     3541 acaaggagat cgaccggacc atcgtgcatc cgggcaagct gccgcccgcc ctgcagtacg
     3601 acatctgccg caagtgccac atgcagggcg ccatggtgct gcgggacggg cgctcgccct
     3661 tcgacttccg gccgggcaag cggctcacgg actacatgga tacgttctgg ccgcggtttc
     3721 ccgactcggc gcggagcttc atcatggcgt ctcatccgga tcggctgcag atgagcgcgt
     3781 gcttccgggc ctcgtgggac gaatcgcgcg gactccccga gatgacctgc gtgacgtgcc
     3841 acaatccgca cctcgcggtc gagctcaagc cgtccttcga gtacaacgcg ccgtgccgcg
     3901 cgtgccacgc gcgccccgac ctgccggact gcacggcctc cgaggagcat cgcctcgagg
     3961 tccaggacga ctgctcctcg tgccacctgc cggaggcgtg gaccatcgac atcccgaacg
     4021 tccgcattac ggaccactac atccgggtgc cggaggcgga ccgcgatccc acgccggatt
     4081 acgcgcagca gacgaatctc gtgcgcatgg cgagcctgct ccgcgactcg gtcgacctgc
     4141 ccacgctcgc ccgcggatac ctggcgtact acgaggagtt cgcgcggcga cccttcctgc
     4201 tcgactcggc cgcggccgtc atcgagcgtg cgcccgaggg cgattccctg cgcgagcgcg
     4261 tcctggtgcg cgtgctgtac ctgcgcgagg aatgggaccg gctcaggcgg ctcgccgggg
     4321 gacgcgaccc ggccgcggag cacgacgcct ggaccgccta ccggatcggg gaggcgttca
     4381 tgaaggcgga ggactatccc tcggcgctcc ggtggttcga tcgcgtcgcg gacctcgctc
     4441 ccgaccacct gcggttcatg gaccggctgg gattcgcctt gatgaacgcc ggccggccgg
     4501 aggatgcggt cgccgtcctg gacggggtgc tcgagcgcga cccgacgctg gcccgcagcc
     4561 gcaacaaccg cgggttcgcg cgcctggtga tcggcgagct cgaggcgggc gaagatgacc
     4621 ttcgcgaggc gatccgcctg gaccccgatt tcgccgaggc cgtcggcaac ctcgcctcgc
     4681 tcctgctgaa cacgggccgt gcccgcgagg ccatgccgct tgcgcagagg ctggtcacga
     4741 tggaccccgc cgacgatcgc taccgggcgt tcctgcgcca ggtcaccgtg gcggcacagg
     4801 cggagccggt cacgccggag gcaagggaat tgcccagccc gacgaattga cgaggtcggt
     4861 caggacggcg gcggcgccgc gagggggcgt gtggtgttcc gcgcgcgacg gatgcgtcag
     4921 tcggcgcgcc agaagaccag gtcgtccacg cgggcgtacg tcgtcccggt gtacgtgacc
     4981 ccgtagcccg ccatcgtcga gtcggaggtc aaggcgccga agaagtacag ggccgccggg
     5041 cggaccgagg tgcacccgga ggcccgatcg agcagatcgc agtcgtagtc ttcggacgcg
     5101 ggggcggtgt agagagcgaa gcgcagcgaa tcgcccgagt acttctgacc ttccacgaac
     5161 tcggacagcc cgtacaccgg gtcgccctcg agccacgggt cgggatcgat ccactggtag
     5221 acccactccg ctgtccccgc caggtcatcg tcgagctcct gcaggttcac gacggtcgac
     5281 cactgctcgt cggccccgaa gaggtcgttg tgcacgacga aggtcgccga ccaggtcgtc
     5341 tggtccaggg gattggacct ttcgtcgacc gtggtccagt cacagccgcc caggaccatg
     5401 gccaggccca tcaggaggac actggttctc atgtcagcct ccgagttgat tcgtcgtatg
     5461 ctctgcgggc agggttccgg ccgggcgggt tggtcgtaca cggcaccgga aatcgagccg
     5521 ccggggccga ttcccgtctt tgagtgattt atcggccgta tcctgcggaa ggataaatcg
     5581 cgcggcgcgc cgcatggctc ggtagcgact acgccgaacc ctccaccccc gcccatgtcg
     5641 atcctcgtct acggcgcata tggatacacg ggacgcctca tcgtggagga agcgctccgg
     5701 cgcgggcttg agcccgttat tgcagggcgc aacgcctccc ggcttcgcaa gctccgaatg
     5761 cgaaccgggc tcgagacccg gacgtgcagc ctcgacgacg aggccgggct tctcgccatg
     5821 ctgcgcggcg tccgcgtcgt ggtgcacgcg gccggtccgt tcacggtcac gtccaagccg
     5881 atgggccgcg catgcctcgc ggcgggagtc cactacctgg acatcacggg cgaaatcccg
     5941 gtcttcgaga agatggccgc actcgacggc cgcgcccggg agcgcggcat ccttctgatg
     6001 cccggcgtcg gattcgacgt ggttccgagc gactgcctcg cggcccacct gaagcggcgc
     6061 ctgccgaccg ccacccggct ttcgctcgca atcgcccggg aggggagcgg catgtcgcgg
     6121 ggctcgcttc ggaccgtcct ccgccacctt ggcgggggga gcatcgagcg cgtggacggc
     6181 aacctcgtgc ccgcgccgct cggcgacaag cgcatggagg tcgacttcgg atcagggccg
     6241 cgccgtgtaa tttcggcccc gtggggcgat ctcggcagcg cgtaccactc cacgggcgtc
     6301 ccgaacatca cgacgtactg ggcctaccca ccgctcatgg cccaggccgc caggaggctc
     6361 cactggtttc ggccgctgct gcgcacgagg gccgcgcaga tactgctgca ccgggcggca
     6421 ggcctcttcc attccgaggg cccggacgcg cgggcgcgcg agactggccg cagcctggtg
     6481 tggggcgaga tgcgggacga cgagggccac tccgtccggg cacgcttccg cggcccggag
     6541 atctatgcgc tgaccgcccg cctcgccgcg gacatcgccc tgcgcgtccg ggacggggag
     6601 gggcgccccg gattcgcgac gccgtcgatg ctcttcggcc cggactacat cctgggattt
     6661 ggcggagtgg tcagggaaga cctgtagggg ggatgctcta ttcccctgcg tgcgccccgg
     6721 gcggaatgcg cgattttcgt cgccaccgtt ccgaagggag tactcggcat gttccgcgca
     6781 ctgatcctgg ccttgatcgt cacgatatcg gccagcctgg acgcatcagc acgacagacc
     6841 accggcttct accgtgtaga agggaagaag gtcgtcgacg cgaacggcca gccggccatg
     6901 cgacgcggca tcgggctcgg gggctggctg atgccggaag ggtacatgct gcatttcccc
     6961 ggccacggct cggcgaccaa catgcgcagc aaggttcgcg atctgatcgg cgactcggac
     7021 gcggccgagt tctggaggct ttaccgggag aattacgtgg acgaaaaaga catcgcagcg
     7081 ctcgcggcag cgggattcga ccacgtcagg ctcccgatcc actacgacgt ggtctacgat
     7141 ctggcgtcac gcacgttcaa gcctgagggc tacgagttgc tcgacacctt tatcgactgg
     7201 tgccgcaccc atggactcgg cgtgatcctg gacctgcatg ccgcgccggg agcccagaac
     7261 gagggcgaga tctcggatag cgacgggcag gcgcgactgt ggacgcagcc gaatccgtac
     7321 cagggcatca ctgcggcgat ctggggggag accgccaggc gatactcgaa cgagacccag
     7381 atcatcggct acgatctgct caacgagccg gtcgtcacga ccgacatggt aacggacccg
     7441 ccggcggcgc ttcgcgcgct gtacgagctg ctcacgacgg ccgtccgttc cgaggacccg
     7501 aaccacatcc tcttcatcga gggcaactac tgggcgacga cgttcgacaa tctcacgcca
     7561 cccttcgacg acaagctggt ctatgccttc cataagtact ggaatgcgac ggacatcggc
     7621 acgatccggt acctgctggc catcagggac acgtacgacg tgccgctctg gctcggggaa
     7681 tcgggtgaga acagcaacgc ctggttccgg gccgtcgtcg acctgatgga agaggaggcg
     7741 atcgggtgga attggtggac gcacaagaaa atccagtcaa ccacctcgcc ttggtccgcc
     7801 ctcatcaagc ccggataccg gaccgtgctg gactactggg cggggagtgg tggcaggccc
     7861 tcggtccagg ccgcgcggga aggtctcttc gagatggccc ggggtctgga ccttgattcc
     7921 acgcgcttcg atcccgacgt actggccgct ctcttcgatc cgacgttcgg cgagacgggt
     7981 cacccgtacg cagatcacgt gatccccggg gtcgtgaatg cagcggacta cgataccggt
     8041 cttccgggta ctacctactc ggacagggac aactgggcag tcagcggctc gcctggcgga
     8101 gggaac
//
LOCUS       DBNHQZ010000215         4507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_200266, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000215 DBNHQZ010000000
VERSION     DBNHQZ010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_200266"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="AC16YU_05745"
     CDS             <1..1399
                     /locus_tag="AC16YU_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6064759.1"
                     /translation="SLKALRDLDNSVIVVEHDREMIESADFVVDLGPGAGEHGGEIIG
                     AAAPHLLPVGTNGHASLTTAYLRGERRIAVPRERREGNGKALELRGATGHNLKGDDLR
                     LPLGRFICVTGVSGSGKSSLVNQTLYPLLANHFHHAKAVPLPHAGVDGLEHVDKVIDI
                     DQSPIGRTPRSNPATYTGLFGHIRDLFAQMPESQIRGYKSGRFSFNVKGGRCETCKGA
                     GIVKLEMNFLPDVYVGCETCRGRRYNAETLEVRFKGRSIADVLEMTVSEALVLFEHVP
                     RIERKLRTLASVGLGYIRLGQQATTVSGGEAQRVKLAKELSRPGTGSTLYILDEPTTG
                     LHFEDIRHLIAVLQALVKKGNTVVVIEHNMEVVKVADHVIDLGPDGGDAGGRIVAQGT
                     PEDVAATDTHTGRFLRLELDRARTAVPGEEEVDLDLDAFAGDDGSDEDDEPDDESDDE
                     PDAAPGAAPGDGPGP"
     gene            1461..1853
                     /locus_tag="AC16YU_05750"
     CDS             1461..1853
                     /locus_tag="AC16YU_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064760.1"
                     /translation="MEPTYVPEHRKPDAPGIAMNFDNTVSLYQVVGEDESFEQAARAA
                     FALLKEAQERYPDWPRLFYVDIAGHRGPRSGFDEDFFEFQQEFWFSTVAHFVTAFDTP
                     LTGPLLNPAGQRNDVPDSLDIPSGDRPS"
     gene            1850..3193
                     /locus_tag="AC16YU_05755"
     CDS             1850..3193
                     /locus_tag="AC16YU_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6064761.1"
                     /translation="MKRFALVLSLGLVTFGPSSAQPVPDMPVILGAREQARVVDHWLE
                     RRLDTVVPALMRREGIDLWIVASREYNEDPAMHSMLPATWLAARRRTILVFHDRGGEG
                     GVERLAVARYAIGSLFEGAWNPDEQPDQWARLAEILAERDPERIAVNRSTDFAHADGM
                     THTEFEAFEAALPERLHGRIVSGERLAVGWLETRIPEEMDVYPRIVRIARTIIREGLS
                     ARAIQPGVTTTADVEWWYRDRIRELGLDTWFHPSVDVQRAESGGRGDDFSSRPGEQVI
                     VPGDLLHVDFGITYLRLNTDTQQHAYVLRPGETDAPEGLKRALATGNRLQDILMAEFA
                     EGRTGNEILAGALAAAKAEGIRPSIYTHPVGLHGHAAGPTIGLWDRQDGVPGPGDYPV
                     FANTAYSIELNALVSVPEWGGQDVRIMLEEDAFFDGQSMRWIDPRQEALYLVPGG"
     gene            complement(3197..3937)
                     /locus_tag="AC16YU_05760"
     CDS             complement(3197..3937)
                     /locus_tag="AC16YU_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064762.1"
                     /translation="MIPRIATLLFCLTLAACGGRTDEPATHEMDPGAAPMADADMAPD
                     ASNPMGNPELLVPPGWTARYDDGQDRPLGADDSAEVYLVTMTPGWHVTTGPAGIFWHP
                     ASTASGAYSVRTVVHLFPPGDRNEGYGLFFGGTGLEGPDQAYVYFLLRRSGEFLVKQR
                     MGEETEELIGWTASDAIVPYADTTQGSIRNELGVNVGVDSVRFSVNGTQVAALAAEGL
                     ATDGTVGLRVNHRLNLHVEDLSVEPAGM"
     gene            4134..>4507
                     /locus_tag="AC16YU_05765"
     CDS             4134..>4507
                     /locus_tag="AC16YU_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064763.1"
                     /translation="MISPLDALARQAASGQGEVHLVHWPDVVYETQGAMDIPSMRAAD
                     QYGELAVLPRVDTLALRYAWSIVDGLPRIDFELRWVRGSGGIVDGRLVADEEMPETIL
                     LDLADLHADVVVRGERVGDLVLS"
BASE COUNT          674 a         1557 c         1600 g          676 t
ORIGIN      
        1 ctcgctgaag gccctgcgcg acctggacaa ctccgtcatc gtggtcgagc acgaccgcga
       61 gatgatcgaa tccgcggact tcgtcgtgga cctcgggcct ggcgcgggcg agcacggcgg
      121 cgagatcatc ggcgcggccg cgccccatct tctccccgtg ggaacgaacg gccacgcgtc
      181 gctcaccacg gcctacctgc ggggcgagcg gcgcatcgcc gtcccgcgcg agcgccggga
      241 ggggaacggg aaggccctcg aactccgggg ggcgaccggc cacaacctga agggcgacga
      301 tctgcggctg cccctcgggc gcttcatctg cgtgacgggc gtctcgggat ccggcaagtc
      361 cagcctcgtc aaccagacgc tctacccgct gctggccaac cacttccacc acgcgaaggc
      421 ggtgccgctg ccgcacgcgg gcgtcgacgg cctggagcac gtcgacaagg tcatcgacat
      481 cgaccagagt cccatcgggc ggacgccccg ctcgaacccg gccacgtaca cggggctctt
      541 cgggcatatc cgcgacctct tcgcgcagat gcccgagtcg cagatccggg gctacaagtc
      601 ggggcgcttc tcgttcaacg tgaagggcgg acgctgcgag acgtgcaagg gcgcgggcat
      661 cgtcaagctc gagatgaact tccttccgga cgtgtacgtc ggctgcgaga cgtgccgcgg
      721 ccgccggtac aacgccgaga cgctcgaggt ccgcttcaag gggcgctcga tcgccgacgt
      781 cctcgagatg accgtttccg aggccctggt gctgttcgag cacgtgccgc gcatcgagcg
      841 aaagctgcgc acgctcgcct cggtgggcct cggctacatc cggctcggcc agcaggccac
      901 taccgtgtcc gggggcgagg cgcagcgcgt caagctggcg aaggaactct cgcgccccgg
      961 cacgggctcg acgctctaca tcctggacga gccgacgacc ggcctgcact tcgaggacat
     1021 ccggcacctg atcgcggtgc ttcaggccct ggtgaagaag ggcaacacgg tggtggtcat
     1081 cgagcacaac atggaagtgg tgaaggtggc cgaccacgtc atcgacctgg gcccggacgg
     1141 gggcgacgcg ggcgggcgga tcgttgcgca gggcacgccc gaggatgtgg cggcgacgga
     1201 cacgcacacc gggcggttcc tgcgcctgga gctcgatcgc gcccggacgg ccgtccccgg
     1261 agaggaggaa gtggacctgg acctggacgc cttcgcgggc gacgacgggt cggacgagga
     1321 cgacgagccg gacgacgagt cggacgacga gccggacgcc gctcccggcg ccgctcccgg
     1381 ggacggcccc ggcccctgag ccggccttgc cccgttcgtc cggcaccgcg acctttccgc
     1441 cccgcaccga cggaccgtcc atggaaccga cctacgtgcc ggagcaccgg aagcccgatg
     1501 ctcccggcat cgcgatgaac ttcgacaaca cggtctcgct gtaccaggtg gtgggcgagg
     1561 acgagtcgtt cgagcaggcg gcccgggcgg ccttcgcgct cctgaaggag gcgcaggagc
     1621 gctatcccga ctggccccgg ctcttctacg tggacatcgc ggggcaccgc ggcccgcgca
     1681 gcggcttcga cgaggatttc ttcgagttcc agcaggagtt ctggttctcg accgtcgcgc
     1741 acttcgtgac ggccttcgac acgccgctca cgggtccgct gcttaacccg gcggggcagc
     1801 gcaacgacgt ccccgattcg ctcgacattc cttccggcga ccgaccctca tgaaacgctt
     1861 tgccctcgtt ctttcgctcg gcctagtcac cttcgggccc tcttccgccc agcccgttcc
     1921 cgacatgccg gtcatcctcg gcgcccggga gcaggcacgg gtcgtggacc actggctcga
     1981 gcgccgcctg gacaccgtcg tccccgcgct gatgcggcgg gaaggaatcg atctctggat
     2041 cgtcgcctcc cgggaataca acgaggatcc ggccatgcac tcgatgctgc cggccacgtg
     2101 gctcgccgcg cggcggcgca cgatcctcgt gttccacgac cggggcgggg agggcggcgt
     2161 cgagcgcctg gccgtcgcac gctacgccat cgggtccctg ttcgagggcg cctggaaccc
     2221 ggacgagcag cccgaccagt gggcccgcct ggccgagatc ctcgccgagc gcgacccgga
     2281 gcggatcgcc gtgaaccggt cgaccgattt cgcgcatgcc gacggcatga cgcacacgga
     2341 gttcgaggcc ttcgaggccg cgctcccgga gcggctccac gggcgcatcg tgtccgggga
     2401 gcgcctcgcg gtcggctggc tcgagacgcg catcccggag gagatggacg tgtacccgcg
     2461 gatcgtgcgg atcgcgcgga cgatcatccg cgagggcctc tccgcgcgcg ccatccagcc
     2521 cggggtgacc acgaccgcgg acgtggaatg gtggtaccgg gaccggatcc gcgagctggg
     2581 cctggacacc tggtttcatc cgagcgtgga tgtccagcgg gccgaatcgg gcggccgggg
     2641 cgacgacttc tcgtcccgcc ccggcgagca ggtcatcgtg ccgggcgacc tgctgcacgt
     2701 ggacttcggc atcacgtacc tgcgcctgaa tacggacacg cagcagcacg cgtacgtgct
     2761 ccgtccgggc gagacagacg cacccgaggg cctgaagcgg gccctggcca ccggaaaccg
     2821 gctacaggac atcctcatgg cggagttcgc cgaggggcgg accggcaacg agatcctggc
     2881 cggggcactc gccgccgcga aggccgaggg catccgcccg tcgatctaca cgcacccggt
     2941 cgggctgcac ggccacgcgg caggacccac gatcggcctc tgggaccggc aggacggggt
     3001 gcccggcccg ggagactacc cggtgttcgc gaacacggcc tactcgatcg aactgaacgc
     3061 cctcgtgtcc gtcccggagt ggggcggaca ggacgttcgg atcatgctcg aggaggacgc
     3121 cttcttcgac gggcagtcga tgcgctggat cgatccccgc caggaggcgc tgtacctggt
     3181 gccgggcggc tgagccctac atccccgcgg gctcgaccga caggtcctcg acgtgcaggt
     3241 tcagccggtg gttgacgcgc aggccgacgg tgccgtcggt cgcgagcccc tcggccgcga
     3301 gcgcggcaac ctgcgtgccg ttcaccgaga agcggacgct gtccaccccc acattcacgc
     3361 cgagctcgtt gcggatcgag ccctgcgtcg tgtcggcgta cgggacgatg gcgtcactgg
     3421 ccgtccaccc gatgagctcc tcggtctctt cgcccatgcg ctgcttgacc aggaactcgc
     3481 ccgagcggcg gagcaggaag tacacgtagg cctggtccgg cccctcgagg ccggtgcccc
     3541 cgaagaagag accgtatccc tcgttgcggt cgcccggcgg gaacaggtgc accacggtgc
     3601 ggacgctgta tgcgcccgaa gccgtgctcg ccggatgcca gaagatcccg gcgggccccg
     3661 tggtcacgtg ccagcccgga gtcatggtga cgaggtagac ctcggccgag tcgtcggcgc
     3721 cgagggggcg gtcctgcccg tcatcgtacc gggcggtcca tcccggggga accaggagct
     3781 ccgggtttcc catggggttc gacgcgtccg gcgccatgtc cgcgtcggcc atcggggcgg
     3841 ctccggggtc catctcgtgc gtcgcgggct cgtcggtgcg cccgccgcag gcggcgagcg
     3901 tcaggcagaa caggagggtg gctatgcggg ggatcatgtc ggcgtgggta tggctgcggg
     3961 ttccgggggc tgtcacgcgg cggttcaacg cgcgggttcc gcgtgcgttc tcggcacgcg
     4021 tcttgcccga ctcctggata tattgccaac gtcacggagg agttgaaatg tggcgggaat
     4081 tggtgtattc gaggacggtc ggccttgggc tggtgctcct gatcgcctcg ccggtgatca
     4141 gtccgctgga cgctctcgcc cgacaggccg catcgggcca gggcgaagtg cacctcgtcc
     4201 actggcccga cgtggtctat gagacgcagg gggcgatgga catcccgtcg atgcgggccg
     4261 cggaccagta cggagaactg gccgttcttc cccgcgtgga cacgctcgcc ctgcggtacg
     4321 cgtggtctat cgtggacggg cttccccgga tcgacttcga attgcgctgg gtccgcggct
     4381 cgggcggcat cgtggatggg cgcttggtcg cggacgaaga gatgcccgag acaatcctgc
     4441 tcgatctcgc ggacctgcac gcggacgtgg tggtccgggg tgagcgggtc ggggacctcg
     4501 tgctctc
//
LOCUS       DBNHQZ010000216         3649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2231763, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000216 DBNHQZ010000000
VERSION     DBNHQZ010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2231763"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /gene="groL"
                     /locus_tag="AC16YU_05770"
     CDS             <1..1235
                     /gene="groL"
                     /locus_tag="AC16YU_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844412.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6064764.1"
                     /translation="SRDIEGKSEIAQVAAISANNDNTIGELIADAFDRVGKEGVITVE
                     EAKGTETTLDVVEGMQFDRGYLSPYFVTDADNMEAILEDAYILIHDKKISAMKDLLPV
                     LEKVAQMGRPLLIIAEDVEGEALATLVVNKLRGTLKVAAVKAPGFGDRRKAMLEDIAI
                     LTGGTVVSEEKGYRLENTTLDYLGQAKRIVLDKDNTTVVDGAGEATQIKARAGQIKQQ
                     IESTTSDYDREKLQERLAKLAGGVAVLKIGAATEPEMKEKKARVEDALHATRAAIEEG
                     IVAGGGVALIRALSALEKLKLENEDQNIGVAIVRRALEEPLRQIAANAGQEGSIVVQK
                     VKEGKGDFGFNARTDEYGNLLEQGVLDPTKVARTALENASSVAGLLLTTEAVIADKPE
                     KESGPSMPPGGGMGGMDF"
     gene            1430..2686
                     /gene="folK"
                     /locus_tag="AC16YU_05775"
     CDS             1430..2686
                     /gene="folK"
                     /locus_tag="AC16YU_05775"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6064765.1"
                     /translation="MEPVEPVVAYIALGSNLGDREENLQAAVTALSDTDGVEVITVSP
                     VYETEAHTGSPDTPAPTFLNAVVSVSTGLAPTALMERLLAIEASRSRRRDPARRWSPR
                     TLDLDMLLYGEGPVRLEGLSVPHPRMGERRFVLRPLADLAPNLHVPEPFDRSVSELLA
                     DCADTAAVFRSDASLLIPGRSLASQGELWPAADDQPVRRQVTRLPEDLRYVVIEGVIG
                     AGKTTLASVISRRFGARLIREQFDENPFLESFYEDRERWAFQTQLSFLASRFRQQQSL
                     GGRDLFHDVVVSDYAFDKDRIFARLNLSGDELQLYDTLFRIMQPSVPVPDLVVYLQST
                     TERLMAQIRARGRVYERNMDPAYIEALNQAYNAFFFHYDRSPLLIVNAARLDFVENSA
                     DLEELLSQIARMHYPGTTFFNPVRPA"
     gene            2698..2934
                     /locus_tag="AC16YU_05780"
     CDS             2698..2934
                     /locus_tag="AC16YU_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064766.1"
                     /translation="MLFKFLWILVIIWIAYRVFVAGRNLVRLALGRDETPLPDGSLDP
                     SRSADRPPQRQEPGRDGARTRSAADVEDARWVDL"
     gene            complement(3059..>3649)
                     /locus_tag="AC16YU_05785"
     CDS             complement(3059..>3649)
                     /locus_tag="AC16YU_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR6064767.1"
                     /translation="VYVTEELTQVIAADFLWRFRPGPFATNLSSSRAIDDVARRHGQV
                     VHRSAVGEINVVRKMQEVGAVLGGEGNGGVILPDLHYGRDALVGAAMVLQHLANENLR
                     LSELRASLPEYHIVKQKVEIGDLDPDVLLARVADRHASERISTEDGVKIDFDEGWVHL
                     RRSNTEPIVRVYSEAADRERAAELGRRFMAELLDLG"
BASE COUNT          605 a         1282 c         1212 g          550 t
ORIGIN      
        1 tcagccgcga catcgagggc aagagcgaga tcgcgcaggt cgcggcgatc tcggccaaca
       61 acgacaacac gatcggtgag ctcatcgccg acgcgttcga ccgcgtgggc aaggaaggcg
      121 tcatcacggt cgaggaggcc aagggcaccg agaccacgct ggacgtggtc gagggcatgc
      181 agttcgaccg cggctacctg tcgccctact tcgtgacgga tgccgacaac atggaggcca
      241 tcctcgagga tgcctacatc ctgatccacg acaagaagat ctccgcgatg aaggaccttc
      301 tcccggtcct ggagaaggtc gcccagatgg gccgtccgct cctgatcatc gccgaggacg
      361 tcgagggcga ggcactcgcc accctcgtgg tgaacaagct ccgcggcacg ctgaaggtcg
      421 cggccgtcaa ggccccgggc ttcggcgatc gccgcaaggc gatgctcgag gacatcgcga
      481 tcctcacggg cggcacggtc gtctccgagg agaagggcta ccgcctggag aacacgaccc
      541 tcgactacct gggccaggcc aagcgcatcg tcctggacaa ggacaacacg accgtcgtcg
      601 atggcgccgg cgaggccacc cagatcaagg cccgtgccgg ccagatcaag cagcagatcg
      661 agtccacgac cagcgactac gaccgcgaga agctccagga gcgcctcgcg aagctcgccg
      721 gcggcgtggc cgtgctcaag atcggcgccg cgaccgagcc cgagatgaag gagaagaagg
      781 cccgcgtcga ggatgcgctc cacgccacgc gtgccgcgat cgaggagggc atcgtcgccg
      841 gcggcggcgt cgcgctgatc cgtgcgctct cggccctcga gaagctgaag ctcgagaacg
      901 aggaccagaa catcggcgtc gccatcgtcc gccgcgcgct cgaggagccc cttcgccaga
      961 tcgcggcgaa cgccggccag gagggctcga tcgtcgtgca gaaggtgaag gagggcaagg
     1021 gtgacttcgg gttcaacgcc cgcaccgacg agtacggcaa cctgctcgag cagggcgtgc
     1081 tcgatccgac caaggtcgcc cgcacggcgc tcgagaacgc gtcgtccgtc gccgggctgc
     1141 tgctcacgac cgaggcggtc atcgccgaca agccggagaa ggagtccggt ccctcgatgc
     1201 ctccgggtgg cggcatgggc ggcatggact tctaggagac aagcgcggga tccggcatct
     1261 cgccgtcggg ggcggggtcg cagaggcggc cccgcccctt tccttttgtc cggaggttcg
     1321 cgcccgccgc gttgcccgcg ccggacgcgc gccctagatt cggcgcaccg ataccgcacg
     1381 agcgcctcat cgcacccgca cgcacatccg gcccgagatc ggcgcttcga tggaacctgt
     1441 agagcccgtc gtcgcctata tcgcactcgg ctccaacctg ggagaccgcg aggagaatct
     1501 gcaggcggcc gtcacggcgc tgtcggatac ggacggcgtg gaggtgatca cggtgtcgcc
     1561 cgtctacgag acggaggccc ataccggatc gcccgatacg ccggccccca cctttctgaa
     1621 cgcggtcgtg tccgtgtcga cgggcctggc gcccaccgcg ctgatggagc ggctgctcgc
     1681 aatcgaggct tcgcgctccc gccggcgcga cccggcccgc cgctggtcgc cgaggacgct
     1741 ggacctcgac atgctgctct acggcgaggg gcccgtgcgg ctggagggcc tgagcgtgcc
     1801 ccatccccgc atgggcgagc gccgcttcgt cctgcgcccg ctggcggacc tggcccccaa
     1861 cctgcacgtg ccggagccgt tcgaccggag cgtttccgag ctcctggccg actgcgccga
     1921 caccgccgcg gtcttccgct cggacgcttc gctgctcatc cccggacgca gtctcgcgtc
     1981 ccagggcgag ctgtggcccg ccgccgacga tcagcccgtc aggcgacagg tcacccggct
     2041 gcccgaggac ctgcgctacg tggtcatcga gggggtcatc ggagcaggca agaccacgct
     2101 cgcgtccgtc atctcgcgcc gtttcggcgc gcggctgatc cgggagcagt tcgacgagaa
     2161 tcccttcctg gagagttttt acgaggaccg ggagcgctgg gcgttccaga cccagctctc
     2221 gttcctcgcg agccggttcc ggcagcagca gtcgctcggc gggcgggacc tgttccacga
     2281 cgtggtcgtg agcgactatg cgttcgacaa ggaccgcatc ttcgcccgcc tgaacctgtc
     2341 cggcgacgag ctgcaactct acgacacgct gttccggatc atgcagccgt cggtgcccgt
     2401 gccggacctc gtggtctacc tgcagtcgac gaccgagcgg ctcatggcgc agatccgcgc
     2461 ccgcgggcgc gtgtacgagc ggaacatgga cccggcctac atcgaggcgc tgaaccaggc
     2521 ctacaacgcc ttcttcttcc attacgaccg ctcgccgctc ctcatcgtga acgccgcgcg
     2581 gctggacttt gtcgagaatt cggcggacct cgaggaattg ttgtcccaga tcgcgcgtat
     2641 gcactatccg ggtaccacgt tcttcaatcc cgtccgaccg gcctgacgcc gggccgcatg
     2701 ctcttcaagt tcctctggat cctggtcatc atctggatcg cgtaccgcgt cttcgtggcc
     2761 ggacgcaacc tggttcggct ggccctgggc cgtgacgaga cgccactccc ggacggctcc
     2821 ctcgatccgt cgcggtcagc ggaccgtcca ccccagcgac aggagccggg ccgggacggt
     2881 gcacgcaccc ggtccgccgc cgacgtcgag gacgcccgct gggtggacct gtgaggtcca
     2941 gcgccggcct ggccgaagga gaaatccggc agatcaaggc aagcgaacgg acgggaatga
     3001 ggcgtatcgc gatacgccgc aatgaccggc cgtgaagctc aacgccgagc tgacggattt
     3061 atccgaggtc caggagttcg gccatgaagc ggcggcccag ctcggccgcc cgctcccggt
     3121 ccgccgcctc gctgtagacc cgcacgatcg gctcggtatt cgagcgccga aggtgcaccc
     3181 agccctcgtc gaaatcgatc ttgacgccgt cctcggtcga gatccgctcc gaggcgtgcc
     3241 ggtcggcgac gcgggcgagc agtacgtccg ggtcaaggtc tccgatctcc accttctgct
     3301 tgacgatgtg gtactcggga agcgaggcgc ggagctccga caggcgcagg ttctcgttcg
     3361 cgaggtgctg cagaaccatg gcggcgccga cgagcgcgtc gcgcccgtag tgaaggtccg
     3421 gcaggatcac gccgccgttt ccctcgccgc ccaggaccgc gcccacctcc tgcatcttcc
     3481 gcaccacgtt gatttcgccg accgccgagc ggtggaccac ctgcccgtgg cgccgggcca
     3541 cgtcgtcgat cgcgcgtgac gatgacaggt tcgtcgcgaa cgggcccggc cggaaccgcc
     3601 acaggaaatc cgccgcgatc acctgggtga gttcctcggt cacgtacac
//
LOCUS       DBNHQZ010000217         7462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2504356, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000217 DBNHQZ010000000
VERSION     DBNHQZ010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2504356"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16YU_05790"
     CDS             complement(<1..653)
                     /locus_tag="AC16YU_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6064768.1"
                     /translation="MRAIARLLPVLLLASLVAPVRAQDGYRVPPDELARLVDAPSTPV
                     VSLSPDRSRLAVLPRQELPTIRDLARPELRLAGLRIDPRNFGQSRAAFSTGITLVRLS
                     DGSESAVTGLPGDVRIGDFAWSPDGSGLAFEHVAEDRIELWVMDVESARARRLTDLRL
                     NGAISGSSFAWAPDGASLLARVVPAGIGEAPAAPEIPATPVIQVAGGDAAPARTYQDL
                     "
     gene            complement(772..1026)
                     /locus_tag="AC16YU_05795"
     CDS             complement(772..1026)
                     /locus_tag="AC16YU_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064769.1"
                     /translation="MSVDSYTLAAFLAGQLPERERRSVSAALVLDPEARELLHMACEA
                     LAAAFQSDEQRNEFMRRIRTGPSAARQDRPAEPAPHRKVA"
     gene            complement(1133..2845)
                     /locus_tag="AC16YU_05800"
     CDS             complement(1133..2845)
                     /locus_tag="AC16YU_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl-peptidase 3 family protein"
                     /protein_id="HIR6064770.1"
                     /translation="MRIILSLAVFPLFAVVAACGDGVPEQSAFSCSEYLASVDPAPTD
                     AAWNLDEGSDEARALLAKYTPFRLTTDLAVLSESECRMLPLLIEAAQAMDEIFWMQAY
                     GDRDSLLAAIEDPGLRTFARINYGPWDRLDNERPFVDGVGPKPDGANLYPASLSREVF
                     ERAATRDSTLRSLYTLVRRGADEGLRAVPYREAFAPQMERASALLQEAATLAEDPGLR
                     AYLEARSRALVTDEYRESDLAWMDMKNNRIDVVIGPIETYEDQLFGYKAAASAYVLVK
                     DMEWSERLSRYAALLPSLQEGLPVHDRYKQEVPGSDSDLNAYDVVYYAGDSNAGSKTI
                     AINLPNDEQVQLQRGTRRLQLKNAMRAKFDRIMAPIADMLIAEDQRAHVTFDAFFGNT
                     MFHEVAHGLGIKNTLNGRGTVREALREHASALEEGKADVLGLYMVDALVERGEYEADV
                     RDHMTTFLAGIFRSVRFGASSAHGRANMIRFNYFREVGAFTRDPETGTYRVDFDRMRE
                     AAYALSEMILRLQGDGNYDGVVFLLEQYGQVGPELQADLDRLAGAGIPVDVVFEQGPA
                     TLAK"
     gene            complement(2935..3630)
                     /locus_tag="AC16YU_05805"
     CDS             complement(2935..3630)
                     /locus_tag="AC16YU_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6064771.1"
                     /translation="MPRFRPPLRRTALAAILVFAGRADAAGQLGRPSPPRGPVVETGS
                     AIAWPDLPPGRRMASGGTYSPGDWVVAHRSLPLGLRLVLTDDRGERAAVVTVADRGSH
                     LASGAMRVSDAAAALLGIPEDEPRRVRFRRLRPGEPATPGVWTPDRAAEPAAGQEWSV
                     QLGSFGERRAAERFAERLDGTRVERIRVDGRTVYRVYFGRFSDRTHAEAWRERLAGWG
                     IDGFVKSLHEADR"
     gene            complement(3635..4726)
                     /locus_tag="AC16YU_05810"
     CDS             complement(3635..4726)
                     /locus_tag="AC16YU_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HIR6064772.1"
                     /translation="MPALLLLLASLATAPADTVTTGHYRVFDGEGNPSSIEAIVEAMA
                     EADVLYIGETHDDPVAHHLQRELLERAFTALHQRGVVLSMEMFTRDVQRVVDEYLAGL
                     ITESHFTGASSPWINYGTDYRPLVEFARIHSIPVVAANAPRRYVNRVTRLGPESLYDL
                     PDYTRESMAPLPYAPASDRYRAEWDTLMAESMAEMQAAADSAAEPGEASRPAMPAMPA
                     MAPAAPDSLPPGHPRPDAADELPPGHPRPEPADSAAAAPAAGPDHGSMAYMLDAQSLW
                     DATMAWSVAEALLRRPGSLVLHVVGAFHVEHGTGIPEHLARYRPGVRQVVVTVRPAAD
                     VEAFDSGEHGGLGDFVILADEALPRTHER"
     gene            complement(4744..6333)
                     /locus_tag="AC16YU_05815"
     CDS             complement(4744..6333)
                     /locus_tag="AC16YU_05815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6064773.1"
                     /translation="MSTEREPGRPYTRSIDLTSRAKLLRQTENAAGSFAEPDPALPEV
                     ELDDLPPALRRSVGTAGWHSLMPVQRMAIPYLLAGRDLIVQSKTGSGKTGAFLLPLFE
                     QLDPSVKAAQALILSPTRELAKQIHEEFERIRGDHPLEAATIYGGVRYGPQLDALERG
                     AQVVVGTPGRILDHLQRRTLKLDRLRWFVLDEADEMLSMGFFPDMMQLKRYLPTPRHS
                     AMFSATIPPRVRTMAGEFLVNPGFLSLSTGTVTVDSVSHRYYRVDRMDKDRALVRLIE
                     MENPESAIIFANTKREVEYLGQFLSNYGYDAAAISGDLSQAAREQVMGRIRRGALRFM
                     VATDVAARGIDITDLSHVFMVDVPKDQEYYVHRAGRTARAGKSGKAMVLATIEDEATL
                     KRIAHKYGFEMERAELPDDDDVAARVNERMTVTLESRFRDKSNLNRERLRRFVPMVEE
                     LAREEPELLAMLIDDLYHATLHGAPEGSRPPQATRPPEGSRPADEEAAPSGERTGRGR
                     KGRRRGRRSDSDRSDSDRSAD"
     gene            complement(6345..7094)
                     /gene="fabG"
                     /locus_tag="AC16YU_05820"
     CDS             complement(6345..7094)
                     /gene="fabG"
                     /locus_tag="AC16YU_05820"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR6064774.1"
                     /translation="MNLDLSGQAALVTGGSRGIGRAVVEALARAGARVAFTYRSSANE
                     ADALAAALATEGLETMAIASDAADPVEAERVVARVTGEWGRLDVLVNNAGINRDRLML
                     RMTEDDWDAVIGTNLKSVFGYSKAAYPVMMRQRSGRIVNMTSVVGVMGNAGQTNYAAS
                     KAGIIGFTKSLARELGSRGVTVNAVAPGYVETDMTAAMPDAAKQAMLGSVPLGRPAQP
                     AEIASAVLFLASSSAAYITGHVLLVDGGMAM"
     gene            complement(7091..>7462)
                     /locus_tag="AC16YU_05825"
     CDS             complement(7091..>7462)
                     /locus_tag="AC16YU_05825"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR6064775.1"
                     /translation="PVSGAFHSPLMTHARDGLAKALADVRIIDPTVPVWLNVTAKPSR
                     DAGEIRKRLLEQLLAPVRWTQTMESMHGAGATRFVEIGAGRVLSGLAKRTIGREAETL
                     ALGTVDDINALTRSDQHREST"
BASE COUNT         1048 a         2643 c         2655 g         1116 t
ORIGIN      
        1 aggtcctggt aggttctggc gggcgccgcg tccccgccgg ccacctggat gacgggcgtc
       61 gccgggatct ccggcgccgc gggcgcctcg ccgatgccgg caggcaccac gcgcgcgagc
      121 agggaagcgc cgtcgggtgc ccaggcgaat gacgatcccg agatcgcgcc gttgagccga
      181 aggtccgtga gcctccgggc gcgggcgctc tccacgtcca tgacccagag ctcgatgcgg
      241 tcttccgcca cgtgctcgaa cgcgagcccg gacccgtccg gcgaccaggc gaagtcgccg
      301 atgcgcacgt cgccgggcag gcccgtcacc gcggattccg atccgtcgga gagccggacg
      361 agcgtgatgc cggtcgagaa cgctgcccgg ctctggccga agttgcgtgg atcgatcctc
      421 aggcccgcca ggcgcagctc gggccgggcg aggtcacgga tcgtggggag ctcctgccgc
      481 ggaagcacgg cgagacgcga ccggtccggc gaaagcgaga ccaccggggt agagggcgcg
      541 tccacgaggc gagccagctc gtcggggggc acgcggtagc cgtcctgcgc ccggacaggc
      601 gccacgagcg atgcgagaag gagaacgggg aggagtcggg cgatcgcgcg catggtcacg
      661 gtccggatcg tggaaacgtg tccgcgaata taccggagcg ggatccgccg taccacgcgg
      721 cgggcggatg gccactgcct ggccccgcct gccctgcggc gagccctcga atcaggcgac
      781 cttgcggtgc ggagcgggct cggccggacg gtcctgccgc gcggcggacg ggcccgtccg
      841 gatcctgcgc atgaactcgt tgcgctgttc gtcggactgg aaggcggccg ccagcgcttc
      901 gcacgccatg tgcagcagct cgcgggcttc ggggtccagg acgagggcgg cagacaccga
      961 gcggcgctcc cgctcgggca gctggcccgc gaggaatgca gccagggtgt aggaatcgac
     1021 ggacatggtc ggggaggttc ggtgcggcga ccttcggata gcggtatcgg ccgccacccg
     1081 ggatcgcttt aaccgacccg tccgagttcc tccacgccgc ccgtcgaggc cgctacttcg
     1141 cgagcgtcgc cggcccctgc tcgaagacga catccaccgg gatgccggcc cccgcaagcc
     1201 ggtccaggtc cgcctggagc tcggggccga cctggccgta ctgctcgaga aggaacacga
     1261 cgccgtcgta attgccgtcg ccctgcaggc ggaggatcat ctccgagagc gcgtacgccg
     1321 cctcgcgcat gcggtcgaag tccacgcggt aggtccccgt ttccgggtcg cgcgtgaagg
     1381 cacccacctc gcggaagtag ttgaagcgga tcatgttcgc cctgccgtga gcgctggacg
     1441 cgccgaagcg caccgagcgg aatatgccgg ccaggaacgt ggtcatgtgg tcccgcacgt
     1501 ccgcctcgta ttcgccccgc tcgaccagcg catccaccat gtacagcccg agcacgtcgg
     1561 ccttcccctc ctcgagggcg gaggcgtgct cgcgcagcgc ctcgcggacg gtgccgcggc
     1621 cgttcagcgt gttcttgatg cccaggccgt gcgcgacctc gtggaacatc gtgttgccga
     1681 agaacgcgtc gaaggtcacg tgggcccgct ggtcctcggc aatgagcatg tccgcgatcg
     1741 gtgccatgat ccggtcgaac ttcgcccgca tcgcgttctt gagctgcagg cgccgggtcc
     1801 cgcgctggag ctgcacctgc tcgtcgttcg gcaggttgat cgcgatcgtc ttggagcccg
     1861 cgttcgagtc gccggcgtag tagaccacgt cgtatgcgtt caggtcggaa tcgctgcccg
     1921 gcacctcctg cttgtaccgg tcgtggaccg gcagaccttc ctggagcgac ggcagcagcg
     1981 ccgcgtagcg cgagaggcgc tcgctccatt ccatgtcctt gaccagcacg taggccgacg
     2041 cggccgcctt gtacccgaag agctggtcct cgtacgtctc gatcggcccg atgaccacgt
     2101 cgatccggtt gttcttcatg tccatccatg cgaggtcgct ctcccggtat tcgtccgtca
     2161 cgagcgcgcg ggagcgggcc tcgaggtagg cgcggagtcc cggatcctcg gcgagcgtcg
     2221 ccgcctcctg cagcagcgcc gacgcgcgct ccatctgcgg ggcgaaggcc tcgcggtacg
     2281 gcactgcccg cagcccctcg tcggcgccgc gccggaccag cgtgtagagc gaccgcaggg
     2341 tcgagtccct cgtcgccgcc cgctcgaaca cctccctgga caggctcgcc gggtacaggt
     2401 tggcgccgtc gggcttcggt ccgaccccgt ccacgaaggg ccgctcgttg tcgagccggt
     2461 cccacggccc gtagttgatc cgcgcgaacg tccggagccc cgggtcctcg atggcggcga
     2521 ggagcgagtc ccggtcgccg tatgcctgca tccagaagat ctcgtccatg gcctgcgccg
     2581 cctcgatgag gagcggcagc atgcggcatt ccgattccga gagcaccgcc aggtcggtgg
     2641 tgagccggaa gggcgtgtac ttcgccagga gggcgcgcgc ctcgtccgag ccttcgtcca
     2701 ggttccaggc ggcgtccgtc ggggcggggt cgaccgacgc gaggtactcg gaacaggaga
     2761 acgccgactg ctcgggcact ccgtcaccac acgcggcgac gacggcgaag agcgggaaga
     2821 cggcgaggga caggatgatc cgcatggctt tccaggttct tgctcgatca gcccacgtta
     2881 ccgggacatc gccgagggat caagcggccc cggtggaaga ccccgtggaa tccgctaccg
     2941 gtccgcctcg tgcaggctct tcacgaagcc gtcgatgccc cagccggcga ggcgctcgcg
     3001 ccaggcctcg gcatgggtcc ggtcggagaa tcgcccgaag tacacgcggt acaccgtgcg
     3061 gccgtccacc cggatgcgct cgacccgggt gccgtcgagc cgctcggcga accgctcggc
     3121 ggcccttcgc tccccgaacg aacccagctg cacggaccac tcctgccccg ccgccggctc
     3181 cgcggcccgg tcgggcgtcc acactcccgg cgtcgccggc tcgcccggtc gcagcctccg
     3241 gaagcgtacc cttcggggct cgtcctccgg aatgcccagg agggcggccg ccgcgtccga
     3301 aacccgcatg gctccggagg cgaggtgcga cccgcggtcc gcgacggtga ccacggcggc
     3361 gcgctcgccc ctgtcgtcgg tcaggacgag ccgaaggccc agcggcagcg agcggtgcgc
     3421 cacgacccag tcccccggac tgtaggtccc gccggaggcc atccgccgtc ccgggggaag
     3481 gtccggccag gcgatggccg atcccgtctc gacgaccggt cccctcggcg gagagggcct
     3541 ccccagctgg cccgcggcgt cggcccggcc cgcgaagacg aggatcgccg cgagggcggt
     3601 ccttcgaagg ggcgggcgaa agcgcggcat ggtcctaacg ctcgtgcgtg cggggaaggg
     3661 cctcgtcggc caggatcacg aaatcgccca ggccaccgtg ctccccggaa tcgaacgcct
     3721 cgacatccgc cgccggccgg accgtgacga cgacctggcg gacgcccggg cgataccgcg
     3781 cgaggtgctc tggaatgccc gtgccgtgct ccacgtggaa cgcgcccacc acgtgcagga
     3841 ccaggctgcc cggcctgcgg agcagggcct cggccacgga ccaggccatg gtggcgtccc
     3901 agagggactg cgcgtcgagc atgtaggcca tgcttccgtg gtcgggtccc gccgcgggag
     3961 cggccgccgc gctgtccgcc ggctcgggcc gcggatggcc cgggggcagc tcgtccgccg
     4021 catcgggccg cggatggccc gggggcaggc tgtcgggcgc ggccggcgcc atcgcgggca
     4081 tggccggcat ggcgggccgg gacgcctcgc ccggctcggc cgcggagtcg gccgccgcct
     4141 gcatctcggc catgctctcc gccatgagcg tatcccactc ggcgcggtac cggtccgacg
     4201 ccggggcata cgggagcggg gccattgatt cccgcgtgta gtccggcagg tcgtacaggg
     4261 actcggggcc gagacgggtc acgcggttca cgtaccggcg cggggcgttc gccgccacga
     4321 ccgggatgga gtggatccgc gcgaactcca cgagcggccg atagtccgtc ccgtagttga
     4381 tccagggcga cgaggccccg gtgaagtgac tctccgtgat gagaccagcc aggtactcgt
     4441 ccaccacgcg ctgcacgtcc cgggtgaaca tctccatcga caggactacc ccccgctggt
     4501 gcagcgcggt gaaggcccgc tcgagcagct cgcgctgcag gtgatgcgcc acggggtcgt
     4561 cgtgcgtctc cccgatgtac aggacgtcgg cttcggccat ggcctccacg atcgcctcga
     4621 tcgacgacgg gttgccctcg ccgtcgaaga cccggtagtg gccggtggtc acggtgtcgg
     4681 ccggagccgt ggcgagcgag gcgaggagca ggaggagtgc gggcatgcgg gtggtggctg
     4741 gggtcagtcg gcggagcggt cggagtcgga gcggtccgag tcggagcggc gaccgcggcg
     4801 gcggcccttc cgcccccgcc cggtccgctc tcccgatggc gcggcctcct catcggcagg
     4861 ccgggagccc tcgggcggcc gggtggcctg gggcggccgg gagccctcgg gcgcgccgtg
     4921 aagggtcgca tgatagagat cgtcgatgag catggcgagc agctcgggct cctcgcgggc
     4981 cagctcctcg accatgggca cgaagcggcg caggcgctcg cggttcaggt tcgacttgtc
     5041 ccggaaccgg gattcgagcg tgacggtcat gcgctcgttg acgcgggcgg ccacgtcgtc
     5101 gtcgtcgggc agttcggctc gttccatttc gaatccgtat ttgtgggcga ttcgcttgag
     5161 cgtcgcctcg tcctcgatcg tggcgagcac catggccttg ccggacttgc cggcccgggc
     5221 cgtgcggccg gcacggtgca cgtagtattc ctggtccttg ggcacgtcga ccatgaagac
     5281 gtggctcagg tccgtgatgt cgatgccccg ggcggccacg tcggtcgcga ccatgaaccg
     5341 gagggcgccg cggcggatgc ggcccatcac ctgctcccgt gcggcctgcg aaaggtcgcc
     5401 cgagatggcc gccgcgtcgt agccgtagtt cgacaggaac tggcccaggt actcgacctc
     5461 gcgcttcgtg ttcgcgaaga tgatggcgct ctccgggttc tccatctcga tcagccgaac
     5521 gagcgcccgg tccttgtcca tccggtccac ccggtagtac cggtgactca cggagtcgac
     5581 cgtcacggtc cccgtggaca gcgacaggaa cccggggttc accaggaact ccccggccat
     5641 cgtccggacc cggggcggaa tggtcgcgct gaacatcgcc gagtgccggg gcgtgggcag
     5701 gtagcgcttc agctgcatca tgtccgggaa gaaccccatc gacagcatct cgtccgcctc
     5761 gtcgaggacg aaccacctca gccggtcgag cttcagcgtg cggcgctgca ggtggtccag
     5821 gatgcgcccc ggggtcccga ccacgacctg cgcgccgcgc tcgagggcgt cgagctgcgg
     5881 gccgtagcgg acgccgccgt agatcgtggc ggcctcgagc gggtggtcgc cccggatccg
     5941 ctcgaactcc tcgtggatct gcttcgccag ctcgcgggtc gggctcaaga tgagcgcctg
     6001 tgccgccttc acggacgggt cgagctgctc gaacaggggc agcaggaacg ctccagtctt
     6061 gcccgacccg gtcttcgact ggacgatcag gtcccgtccg gcgagcaggt acggaatcgc
     6121 catccgctgg acgggcatga ggctatgcca cccggcggtt ccgacggacc ggcgcagcgc
     6181 cggaggcagg tcgtccagct ccacctccgg aagcgccggg tccggctccg cgaacgaacc
     6241 ggcggcgttc tccgtctgcc gcagcagctt cgccctggac gtgaggtcta tgctccgggt
     6301 gtagggtcgg cccggctctc tttctgtaga catcttcgaa tcgattacat ggccatgccg
     6361 ccgtccacca acagcacgtg tcccgtgatg taggcggccg acgacgaggc gagaaacagc
     6421 acggccgagg cgatctcggc gggctgcgcg ggccggccca gcggaacgct gcccagcatg
     6481 gcctgcttcg ccgcatcggg catcgcggcc gtcatgtctg tctcgacgta gccgggggcg
     6541 acggcgttga ccgtcacgcc ccgggagccg agctcccggg ccaggctctt cgtgaacccg
     6601 atgattccgg ccttggacgc cgcgtagttg gtctgtccgg cgttgcccat gacgcccacg
     6661 acggaggtca tgttcacgat cctccccgac cgctgccgca tcatcacggg atacgccgcc
     6721 ttcgagtacc cgaagacgct cttcaggttg gtgccgatca cggcgtccca gtcgtcctcg
     6781 gtcatccgga gcatgagccg gtcccggttg atgcccgcgt tgttcacgag cacgtccagg
     6841 cgcccccact ccccggtgac gcgtgcgacc acgcgctcgg cttcgaccgg atcggccgcg
     6901 tcgctcgcga tggccatcgt ctcgaggccc tccgtcgcaa gcgcggccgc cagggcgtcc
     6961 gcctcgttcg ccgagctccg gtacgtgaag gccacccgcg cgccggcccg ggccagggcc
     7021 tccacgaccg cccggccgat gccgcgcgat ccgccggtca cgagggcggc ctgtccggaa
     7081 aggtccaggt tcatgtcgac tcccgatgtt ggtccgatcg tgtcagcgcg ttgatgtcgt
     7141 cgacggttcc gagggcgagc gtctccgcct cgcgcccgat cgtgcgcttc gcgaggccgc
     7201 tgagcacccg tccggcgccg atctcgacga agcgcgtcgc accggccccg tgcatggact
     7261 ccatggtctg cgtccagcgg accggggcca gcagctgctc gaggagacgc ttgcggatct
     7321 cgcccgcatc ccgcgacggc ttcgccgtga cgttcagcca taccggcacg gtcgggtcga
     7381 tgatccgcac gtccgcgagc gccttcgcca ggccgtcccg ggcgtgcgtc atgagcggcg
     7441 agtggaacgc gccggacacc gg
//
LOCUS       DBNHQZ010000218         5564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3044482, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000218 DBNHQZ010000000
VERSION     DBNHQZ010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3044482"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /gene="menC"
                     /locus_tag="AC16YU_05830"
     CDS             complement(<1..829)
                     /gene="menC"
                     /locus_tag="AC16YU_05830"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR6064776.1"
                     /translation="MDALTPGTAGGGRITGASAVPFSVPLATSHASSGGRRDGWLLRL
                     EHGAAAGWGEASPLPGFSTESPEAAGEALARLCRRLAGAEVDWSDPLRLPALDAEPLP
                     PSVRFAAELALVNLSAAVTGIPAARLLDPRAGDACPVAALLAGPTDACEARAGEIAAL
                     GFRAAKLKIGSADPAGDAERARAVAARLGPGTALRLDANRAWSLAEAMRFAGAVEGVA
                     IEYLEEPLSDPSQLPGFAAGTGLPVALDESLVGISPGAVAPFDGLRALVLKPTVLGGL
                     "
     gene            complement(817..1722)
                     /locus_tag="AC16YU_05835"
     CDS             complement(817..1722)
                     /locus_tag="AC16YU_05835"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIR6064777.1"
                     /translation="MTGARSGGWLPAPRVWWLAARPRTLWAGVAPVLVGWAMAAADGL
                     FHGPSAVLALVGSILIQVGTNFHNDVADFEKGTDRGDRRGPLRVTAAGLAAPAAMHRA
                     TALVFGAAVVAGVYLMIRGGWPIVIVGFASILFGYMYTAGRRSLAYLGIADLFVLLFF
                     GPVAVAGTYWVQALDLPAAVVVAGFGPGLLAVAILLVNNIRDVDEDRSAGKRTLVVRV
                     GRRTGAWLLWACVAVASAVPVYLVAATGTHPWALAAAVVLLPGTVLAMRVARTDDAAT
                     LNALLGRTAQLGLAWSLAFSLGWML"
     gene            complement(1719..2543)
                     /gene="menB"
                     /locus_tag="AC16YU_05840"
     CDS             complement(1719..2543)
                     /gene="menB"
                     /locus_tag="AC16YU_05840"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR6064778.1"
                     /translation="MSSIDWQESGRYTDIRYHTADGIAKITINRPEVRNAFRPLTVTE
                     MRHALQVARDDHSIGVVILTGEGPDAFCSGGDQKIRGDAGYEEEGTGVMRLNVLDLQR
                     EIRTCPKPVVAMVAGWAVGGGHVLHVVCDLTVAADNARFMQTGPRVGSFDGGYGSSYL
                     ARIVGQKKAREIWYLCRPYDAQQALQMGLVNTVVPLERLEEETVQWCREILEKSPLAI
                     RCLKAAMNADCDGQAGLQELAGNATLLFYMTEEGQEGRNAFNEKREPDFGRFPRRP"
     gene            complement(2530..3339)
                     /gene="menH"
                     /locus_tag="AC16YU_05845"
     CDS             complement(2530..3339)
                     /gene="menH"
                     /locus_tag="AC16YU_05845"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HIR6064779.1"
                     /translation="MSGPLPLSAWGPPDGPPLVLLHGFMGRGEDWAPLAPLLADPWRV
                     VALDLPGHGTATGLPPEAYTLEGAARTAIDALDDLGARRAPLVGYSMGGRIACHVALA
                     APERVEALVLESAHPGLSSTEDRTARLAQDRDRARRLREDFRAFLEEWTADPLFGLAE
                     GDPGRRALVEDRLRGDPAELARALDGLGTGNQTPVGERLRALGVPTLLVAGARDVRYA
                     RLLGELAADCGFSLHVEPGAAHAVHRERPDAFARAVRTFLERTIPTQHVLD"
     gene            complement(3336..4604)
                     /gene="menD"
                     /locus_tag="AC16YU_05850"
     CDS             complement(3336..4604)
                     /gene="menD"
                     /locus_tag="AC16YU_05850"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF027896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylate synthase"
                     /protein_id="HIR6064780.1"
                     /translation="MLTSVDQAVYRSGGTHPGPVHLNAMFRKPLEPQGPESDFAAVDG
                     LGPWASGRSAFTSYAAPRVFPDAGDAAAALRTCERPLIVAGRLAPREAAAVRELAAET
                     GWPVAADVTSQLLLGPDPVLRHTDLLAAHADALPAPDAVIEVGRPGVSGRLRTWLASC
                     GARMHAVVADGPARIDPEHRATHRLEGVLPALEALQAVPARRPWAAAWHEADRRVGEA
                     LGARFGDGGLSEPSAAWHVSSMIPAEHGLWLASSMPIRDMNAFSSAGQAPRLVAANRG
                     ASGIDGTIASAAGLADGLGTPVTLMIGDLAFLHDLNALALLRERPVTLVLLNNDGGGI
                     FSFLPIAEHGDVFEPWFTAPHGLTFEAAAGQFGLDYYAPRDAERFRAAYARALASGRG
                     SVIEVRSERAANRRLHVQIAAEASRLVRAR"
     gene            complement(4753..5004)
                     /locus_tag="AC16YU_05855"
                     /pseudo
     CDS             complement(4753..5004)
                     /locus_tag="AC16YU_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
     gene            complement(5033..>5564)
                     /locus_tag="AC16YU_05860"
     CDS             complement(5033..>5564)
                     /locus_tag="AC16YU_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR6064781.1"
                     /translation="VADAAGDEALRSDLLQSDKEQREHLSVRWAIEETLRPFCELLSV
                     DARATEMRLAQGRHLVSRFEGVLRAGVSSWDLLRHLHPTPATGGYPGPAALKAIRRAE
                     GFDRGWYAGPVGCVGREFAEFAVALRCGLVVGSDLHLYSGAGIVLGSEPEAEWDEIEH
                     KIADFVRALRLAPDPV"
BASE COUNT          821 a         2048 c         2045 g          650 t
ORIGIN      
        1 cgaggccacc gaggaccgtg ggcttcagca cgagcgcccg aaggccgtcg aaaggggcaa
       61 cggccccggg cgagatgccc acgagcgatt cgtcgagggc gacgggcagc cccgtcccgg
      121 ccgcgaaacc gggaagctgc gacgggtcgg agagcggttc ctcgaggtac tcgatcgcga
      181 cgccttccac ggcgcccgcg aagcgcatgg cctcggcgag cgaccaggcc cggttcgcgt
      241 cgagccggag cgccgttccc gggccgagcc gcgcggccac ggcgcgcgcc cgctcggcgt
      301 ccccggccgg gtccgccgat ccgatcttca gcttggcggc ccggaatccg agcgccgcga
      361 tctccccggc gcgggcctcg caggcatccg tcgggccggc gagcagggcg gccacggggc
      421 atgcgtcccc ggcccgggga tcgagcagcc gcgctgctgg aatgcccgtc acggcggccg
      481 aaaggttgac cagggcgagc tcggccgcga accgcacgga cggcggcagc ggctccgcgt
      541 cgagagcagg caggcgcagc ggatccgacc agtcgacctc ggcgccggcc agccttcggc
      601 acagccgcgc cagggcctcc ccggcggcct cgggactctc ggtcgagaac ccgggaagcg
      661 gcgatgcctc cccccagccc gcggccgcgc cgtgctccag ccggaggagc cagccgtcgc
      721 gccgcccccc ggacgaggcg tgggacgttg cgagcggaac ggagaacggt acggccgacg
      781 cgcccgtgat ccgtccgcct cccgcggttc cgggggtcag agcatccatc cgagcgagaa
      841 ggcgaggctc caggccagac ccagctgcgc ggtccggccc agcagagcgt tgagggtggc
      901 cgcgtcgtcg gtccgggcga cgcgcatggc gaggaccgtt ccgggcagca gcacgacggc
      961 cgcggccagg gcccacgggt gggtgcccgt cgccgccacc aggtaaaccg gaacggccga
     1021 ggcgaccgcc acgcacgccc acaggagcca ggcgcccgtc cggcggccca cccggaccac
     1081 aagggtccgc tttcccgccg agcgatcctc gtccacgtcc cggatgttgt tcacaagcag
     1141 gatggcgacc gcaagcaggc ctggaccgaa gcccgccacc acgacggcag cgggcaggtc
     1201 gagcgcctgc acccagtacg taccggcaac ggccaccggc ccgaagaaga gcagcacgaa
     1261 aaggtccgcg atccccaggt acgcgagcga ccgccggccg gccgtgtaca tgtagccgaa
     1321 gaggatcgac gcgaagccga cgatcacgat gggccatccg ccgcggatca tcaggtagac
     1381 gccggccacg accgccgcgc cgaagaccag ggccgtcgcc cggtgcatcg cggccggcgc
     1441 cgccaggccg gccgccgtca cgcggagagg gccgcggcgg tctccgcggt cggtgccctt
     1501 ctcgaaatcg gccacgtcgt tgtggaagtt cgtgccgacc tggatcagga tcgaaccgac
     1561 gagggcgagc acggcagacg gcccatggaa caggccgtcc gccgccgcca tcgcccagcc
     1621 caccaggacg ggcgccacgc ccgcccagag cgtcctgggc cgcgccgcga gccaccagac
     1681 gcgcggcgcg ggcagccagc cgccggatcg cgctccggtc acggccgcct cgggaagcgg
     1741 ccgaagtcgg gctcgcgctt ctcgttgaac gcgttgcgcc cctcctgccc ctcctcggtc
     1801 atgtagaaga gcagcgtggc gttgccggcc agctcctgca gcccggcctg gccgtcgcag
     1861 tccgcgttca tggccgcctt gaggcaccgg atcgcgagcg gcgatttctc gaggatctcg
     1921 cggcaccatt ggacggtctc ctcctccagg cgctccaggg gcacgaccgt gttcaccagg
     1981 cccatctgca gggcctgctg cgcgtcgtac gggcggcaca ggtaccagat ctcccgcgcc
     2041 ttcttctggc cgacgatccg ggcgaggtag ctcgacccgt accccccgtc gaacgagccg
     2101 accctcggtc cggtctgcat gaaccgggcg ttgtcggcgg cgacggtcaa atcgcacacg
     2161 acgtgcagga catgtccgcc cccgacggcc cagccggcga ccatcgcgac gacgggcttc
     2221 gggcacgtac ggatctcgcg ctgcaggtcg agcacgttca gcctcatgac cccggtcccc
     2281 tcctcttcgt acccggcgtc gccgcggatt ttctggtctc cgcccgagca gaaggcgtcc
     2341 gggccctccc cggtcaggat gacgacgccg atcgagtggt cgtcgcgcgc cacctggagg
     2401 gcgtgacgca tctcggtcac ggtcagggga cggaaggcgt tccggacctc gggacggttg
     2461 atcgtgatct tggcgatgcc gtcggccgtg tggtaccgga tgtcggtgta ccggccggac
     2521 tcctgccagt caatcgagga catgctgcgt tggaatcgtt cgttcgagaa aggtgcggac
     2581 ggcccgcgcg aaggcatcgg ggcgttcccg gtgcacggcg tgcgcggcgc cgggctcgac
     2641 atgcagggag aagccgcagt cggcggccag ctcgccgagg aggcgcgcgt accggacgtc
     2701 ccgtgcgccg gccacgagga gcgttgggac ccccagggca cgcaggcgtt ccccgacggg
     2761 cgtctggttc ccggtgccca gtccgtcgag ggcgcgggcg agttcggccg gatcgccgcg
     2821 aagccggtcc tctaccaggg cgcggcgccc gggatcgcct tccgccaggc cgaagagcgg
     2881 gtccgcggtc cattcttcga ggaacgcgcg gaagtcctcg cgcagcctcc tcgcccggtc
     2941 acgatcctgc gcgaggcggg cggtgcgatc ctccgtcgag gagagtcccg ggtgcgccga
     3001 ttcgagcacc agggcctcga cgcgctccgg cgcggcgagg gccacgtggc acgcgatgcg
     3061 gccgcccatc gagtagccca ccaggggtgc ccgacgagcg cccaggtcgt ccagcgcgtc
     3121 gatcgccgtc cgggctgcgc cctccagggt gtaggcctcc ggcggaagac ccgtggccgt
     3181 cccgtgaccc ggaaggtcga gcgcgaccac gcgccagggg tccgcgagga gcggcgcgag
     3241 cggggcccaa tcctcgccgc gccccatgaa gccgtggagc agcaccagcg gagggccgtc
     3301 cggcggcccc caggcggaaa ggggcagcgg gccgctcatc gcgcgcggac caggcgggag
     3361 gcttccgccg cgatctggac gtgcaggcga cggttggcgg cccgttcgct tcggacctcg
     3421 atgaccgatc cgcgcccgga cgcgagcgca cgggcgtacg ccgcgcggaa ccgttcggcg
     3481 tcacggggcg cgtagtaatc caggccgaac tggccggcgg cggcctcgaa cgtcagcccg
     3541 tgcggagcgg tgaaccacgg ttcgaagacg tctccgtgct ccgcgatcgg aaggaaggag
     3601 aagatgccgc cgccgtcatt gttgagcagt acgagcgtca ccgggcgctc ccggaggagc
     3661 gcgagcgcgt tcaggtcgtg caggaaggcg aggtcgccga tcatcagggt gacgggcgtc
     3721 ccgagaccgt ccgcgagccc cgcggcggac gcgatcgtgc cgtcgatgcc gctcgccccg
     3781 cggttcgcgg ccacgagccg gggcgcctgg ccggccgacg agaaagcatt catgtcgcgg
     3841 atcggcatcg agctcgccag ccacagcccg tgctccgcgg ggatcatgga cgacacgtgc
     3901 caggccgccg acggctccga gaggccgccg tccccgaacc gcgcgccgag cgcctcgccc
     3961 acccggcggt cggcctcgtg ccaggccgcg gcccacggcc gccgggccgg taccgcctgg
     4021 agcgcctcga gggccgggag cacgccctcg agccggtgcg tcgcacggtg ctcggggtcg
     4081 atccgcgccg ggccgtccgc gactaccgcg tgcatgcgcg ctccgcacga ggcgagccag
     4141 gtgcggagcc gtcccgacac gccgggccgg ccgacctcga tgaccgcgtc gggcgccggg
     4201 agggcatccg catgcgccgc gagcaggtcc gtgtgccgca ggaccgggtc cgggccgagg
     4261 agcagctgcg aggtgacgtc ggcggcgacc ggccagccgg tttccgcggc aagctcgcgg
     4321 acggccgcgg cctcacgggg tgccagcctg ccggccacga tcagggggcg ttcgcacgtg
     4381 cggagcgccg cggccgcatc cccggcgtcc gggaacaccc tgggcgccgc gtagctcgtg
     4441 aatgccgatc ggcccgatgc ccacggcccg agcccgtcga cggccgcgaa gtccgactcc
     4501 gggccctgcg gctcgagcgg cttgcggaac atggcgttca ggtgcacggg accgggatgc
     4561 gtgcccccgg atcggtacac ggcctggtcc acgctcgtaa gcacgaaggc cggatcgatc
     4621 gccggatccg ggggggggca ggtccacccg ccagcgcacg tacggcgcga agatgggcgg
     4681 ctgctcgatc gcctggttgg ccgccgcgtc ccggagttcg ggaggacggt ctgcggtcag
     4741 gaggacgagg gggacaccgt cgagcgaggc ctcgacgacc gcgggaagcc cgttcgcaac
     4801 ggcggtaccg gaggtcgtga gccaggccgc cggtcggccg gtggcccggg cgtgccccag
     4861 ggcgaagaag gccgctccgc gctcgtccac gtgcagctgc atccgcatcc gcgcattccg
     4921 gacgcacgcg gccacgagcg gcgtggagcg cgagccgggg gcgagagcca cgaggtccac
     4981 gccgcaccgg acgagctctt ccacaaggag gtcggcccag agcctggcga tatcagacgg
     5041 gatcgggggc aaggcgcagc gcgcgaacga aatccgcgat cttgtgctcg atctcgtccc
     5101 attccgcctc cggctccgag ccgagcacga tgccggcgcc ggaatacagg tgcaggtcgg
     5161 acccgacgac caggccgcag cggagagcga cagcgaactc ggcgaactcc ctgcccacgc
     5221 agcccaccgg gcccgcgtac cagccgcggt cgaagccctc cgcccgccga atcgccttga
     5281 gcgccgccgg gccgggatag ccgccggtgg ccggcgtcgg gtgcaggtgc ctgagcagat
     5341 cccacgacga gacgccggca cggagcacgc cctcgaaccg ggacaccagg tgccgtccct
     5401 gcgccagcct catttccgtc gcgcgggcgt caaccgagag cagctcgcag aacggacgca
     5461 gcgtctcctc gatcgcccag cgcacgctca ggtgctcgcg ctgctccttg tcgctctgca
     5521 ggagatcgga ccggagggcc tcgtcgcccg cagcgtccgc gacc
//
LOCUS       DBNHQZ010000219         6853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2232252, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000219 DBNHQZ010000000
VERSION     DBNHQZ010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2232252"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="AC16YU_05865"
     CDS             complement(<1..1519)
                     /locus_tag="AC16YU_05865"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR6064782.1"
                     /translation="MSLQAAPLLALLCLFVPPALASGPGPGDPTGGQVPLPRLAAEIS
                     IDGRLDEPVWLSAAVLRGFSQYLPSDGRPASDSTEVLAWYDATHLYIGIRAYEDHGEV
                     RATLADRDRLSGDDYVTLVLDTFDDRREAYALTVNPFGAQSDGILRDAVRSAGSFGTT
                     SVGGNYSIDTNPDFVFESKGRLTPFGYEIEISVPFKSLRYQSALTQDWGFNVIRKTQH
                     SGYVDTWMPVRQDAASFLAQSGRLSGLTDLRRGLVLDINPEVTGSMAGGPGVDGWEYQ
                     PVDDQYGGNVRWGITNNLVLNGTVNPDFSQVEADVAQFQFDPRRAVFFPEKRPFFLDG
                     SENFASPNPLIYTRQIVNPEAAVKVSGKTGGFTVGVISAMDDRRTSLSGDDLRSFNAL
                     RLRRDLGGQSTLGLVYTDQTDGGTWNRVAAVDGRLVFRSLYSLSWQAAGSFTDLGAGT
                     VAAPLWSVDFSALGRRYGIVAEATGVHGEFVSRTGFISQAGVVNALIQPRYNYYGA"
     gene            complement(1616..2263)
                     /locus_tag="AC16YU_05870"
     CDS             complement(1616..2263)
                     /locus_tag="AC16YU_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6064783.1"
                     /translation="MPEILIVDDHPLTRRGIAVTIESEPNLTVAGEAEDGKTAVDWLS
                     GHEPDLVTVDLSLPDMSGFELVRQIAEAHPNVRVLVISHFENATCAGRALREGARGFV
                     GKSESTEVLVNALTRILDGGIYVSRPIQDRLLRDLMTQKEDGRSPLEVLSTRELDVFE
                     MTGEGLGTREIAERLNLSVKTVESYRRRVRAKLKLPSNPRLMQEAVRWIETRSKC"
     gene            2734..3408
                     /gene="fsa"
                     /locus_tag="AC16YU_05875"
     CDS             2734..3408
                     /gene="fsa"
                     /locus_tag="AC16YU_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR6064784.1"
                     /translation="MKFFIDTADLAEIRDAQAMGLLDGVTTNPSLMKKAGGADFHDHI
                     VRICEIVDGDVSAEVTATDFDGIMAEAHTLAALHPQVVVKVPLIPEGIRAIRSLSGEE
                     IRTNCTLCFSPTQALVAAKAGATYISPFVGRLDDISTSGMELIGQIVQIYENYGYETQ
                     VLAASIRHPLHVVEAALAGADVATIPYSVLMQLMKHPLTDLGLERFLADWEQYRQAAA
                     TSNGAS"
     gene            3430..4872
                     /locus_tag="AC16YU_05880"
     CDS             3430..4872
                     /locus_tag="AC16YU_05880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6064785.1"
                     /translation="MGERDIGPATASFVPLGGTDEIGASCHFLSIDGTGILLDAGADP
                     ELEGTASLPDLDLLRRRTDRWVDHVLVSHAHHDHLGSLPVVIRAFPHVMVHMTRATRD
                     LADILLPASAKLQRRRLVEGSSSAQPLFDTEELGAYSYLYLTHELESPFDATGIRGAT
                     RIEGRLFDAGHVLGSAGIQLTARTPDGPRSLFYTGDTSLRPQTVLPGASYPEGPVDTL
                     IMEATLGGDPDAEQTTRRQEERRLGEALRETLDRGGCAVLPVFALGRGQEIIALLDRF
                     RKNGVIGEDVPIYSAGMLRAIADVYDRTRFSTPRVDTDFQVFGVEQKRLPRGLGGMAE
                     ALARPGIFVVGSGMMFERTLSNRLAQEVVEHERNAVLLVGFAREDSPAHRLMHAAGNG
                     QASEVVLDALRGKQTVRCRVERFRFSGHSHRRDLIGLVERMRPRRVIIVHGETRARAW
                     MADNIRFFHPEVDVCVPDQGQEVPLWPDPD"
     gene            5001..>6853
                     /locus_tag="AC16YU_05885"
     CDS             5001..>6853
                     /locus_tag="AC16YU_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR6064786.1"
                     /translation="MRGRIAGDLRLDTISRALYATDASIYEIMPLAVLLPRTPDDVQA
                     ALLAARAHGVPVLARGGGSSLAGQTVNEALVIDFSQHLDAVLDLDVEAGRIRVQPGIT
                     HERLGRALAGTGWMMGPDPASGSRATLGGMMANNATGTHSILYGNTIRHVERAAAVLA
                     DGTRVDWGAIDADAWRAAIRRTDGEGRVLQGLDMIARGRGEIIRTRMPRHWRRNNGYR
                     VEELLEPSVNPARLLCGSEGTLAVVTDLTVSLVRRPVATAVGVVHFATRREALESVTA
                     ILETDPSAVELFDGAAIGQCRASRGFAHRMTFVEGNPGAILLTEYYGEARAELSDRLD
                     RLEKRILGPTPATGIVRLEDPSAIENAWTVRREALGLIMSVRGNLKPLPFIEDASVPV
                     EHLADYISDLEEVFTETGSEAVMYAHASAGCLHVRPFIDTTRADDVARMERIASASAD
                     LVRRYEGWVSSEHGDGLTRSWLNPVVLGEELYGVCREVKHLFDPEGILNPGRIVDAPP
                     MTGSLRNGPDYAAIPVETRLDFGPEGGLVRAIELCNGNGACRRVGGGTMCPSFMATRE
                     ESDSTRGRANVLRMAMAGRTEGVDWTDPAVYEVLDLCVQCKACKTECPSAV"
BASE COUNT         1055 a         2359 c         2411 g         1028 t
ORIGIN      
        1 cggcgccgta atagttgtac cggggctgga tcagcgcgtt gaccacgccg gcctgcgaga
       61 tgaacccggt gcgcgagacg aactcgccgt ggacgcccgt cgcctcggcc acgatgccgt
      121 accggcggcc gagcgccgag aagtccaccg accagagcgg cgccgcgacc gttccggccc
      181 cgaggtccgt gaaggatccc gcggcctgcc acgacaggga gtagaggcta cggaagacca
      241 ggcgtccgtc gaccgccgcc acccggttcc acgtgccgcc gtccgtctgg tcggtgtaca
      301 ccagtccgag cgtagactgt cccccgagat cccgccggag ccggagcgca ttgaacgacc
      361 gcagatcgtc gcccgagagc gaggtgcgcc ggtcgtccat ggcggagatg acgcccaccg
      421 tgaagcctcc ggtctttccg gacaccttca cggccgcctc cggattcacg atctggcgcg
      481 tgtagatgag cgggttgggg gacgcgaagt tctcgctgcc gtccaggaag aacggccgct
      541 tctcgggaaa gaagacggcg cggcgcggat cgaactggaa ctgggccacg tcggcttcga
      601 cctgcgaaaa gtcggggttc acggtgccgt tcagcacgag attgttcgtg atgccccacc
      661 gcacgttccc gccgtactgg tcgtcaaccg gctggtattc ccatccgtcg accccgggcc
      721 cgcccgccat cgatccggtg acctcgggat tgatgtcgag caccaggccg cgccgcaggt
      781 ccgtgagccc cgacaggcgc ccggactggg ccaggaacga ggccgcgtcc tgccggacgg
      841 gcatccaggt gtccacgtag cccgagtgct gcgtcttccg gatgacgttg aatccccagt
      901 cctgggtcag cgccgactgg tagcgcaggc tcttgaacgg aacggagatc tcgatctcgt
      961 agccgaaggg ggtcaggcgg cccttcgatt cgaagacaaa atcgggattc gtgtcgatcg
     1021 agtagttgcc gcccaccgac gtcgtgccga agctgcccgc cgaccggacg gcgtcgcgga
     1081 ggatgccgtc ggactgcgcg ccgaacgggt tgacggtcag ggcatacgcc tcgcgccggt
     1141 cgtcgaacgt atccaggacc agggtcacgt aatcgtcgcc cgacaaccgg tcgcggtcgg
     1201 cgagcgtggc ccggacctcg ccgtgatcct cgtacgcccg gatgccgatg tagaggtggg
     1261 tcgcgtcgta ccaggcgagc acctcggtgg agtccgacgc gggccgcccg tcggagggga
     1321 gatactggct gaatccgcgc agcacggccg ccgagagcca gacgggctcg tccaaccgcc
     1381 cgtcgatcga gatttccgcg gcgaggcgcg gcaacgggac ctgtcctccg gtcgggtcgc
     1441 ccggtccggg cccggaagcg agtgccggcg gcacgaagag gcacaggagc gccaggagcg
     1501 gtgcggcctg cagggacatg gcaagcgggg gtgaaaacgt gaatttacgc gatctccctg
     1561 cgaagtcccg cttcgccgag gtccaggccg cactccgcag aggatcaggc cgcgctcagc
     1621 acttggagcg ggtctcgatc cagcgcaccg cttcctgcat caggcgcggg ttggacggca
     1681 gcttgagctt ggcccgcacc cggcgccggt acgactcgac cgtcttgacc gacaggttca
     1741 ggcgctccgc gatctcgcgg gtccccaggc cctcgccggt catctcgaac acgtcgagct
     1801 cgcgcgtgga aagcacttcg agcggcgagc gcccgtcctc cttctgcgtc atgaggtcgc
     1861 gcagcaggcg gtcctggatg ggacgcgaca cgtagatccc gccgtccagg atgcgggtca
     1921 gtgcgttaac cagcacctcg gtggactcgg acttgccgac gaagccgcgc gcgccttcgc
     1981 gcagggcccg gccggcgcag gtggcgttct cgaaatgcga gatgaccagg acgcgcacgt
     2041 tcgggtgcgc ctcggcgatc tgccggacga gctcgaagcc gctcatgtcg ggcagcgaaa
     2101 ggtccaccgt cacgaggtcc ggctcatgtc cggagagcca gtcgacggcg gtcttgccgt
     2161 cctcggcctc gccggccacg gtcaggttcg gctcggattc gatcgtgacg gcgatccccc
     2221 gtcgggtcag ggggtgatca tcgacgatga ggatttcagg cacggtgctg atcggatgac
     2281 ccgatggagg agtgagggac ctcggggcac cgccaatgac cgcacagggg agccaccggt
     2341 ttgacagtcg ttcccctcga cattggtaag aaccgtgcgg gcctcggtgt gggacgtaca
     2401 ccggggggcc taccggggaa agccctacag ggcgcgaacg agggatcggt cgaccggact
     2461 atggacgctc cgcctcctcg cggatgcccc gggatgcgta tacttgggga tcgacaccct
     2521 ggctgatcca tgaatcgcaa gctgaagcgt cagttgatca cggcggcggc cattctcgtc
     2581 gcattcgtcg tcatcatggc catcctcagc cggctgggtt gatttcccgc cggccgggtc
     2641 cgaacccgcc cccgggctgg acgttggatg agcggatcgc gccacttccc cccgcgatcc
     2701 gcagtgagcg tgtatcatca tgaccggcca gccatgaagt tcttcatcga tacggcagac
     2761 ctcgccgaga tccgggatgc ccaggccatg ggccttctcg acggcgtcac cacgaatccc
     2821 tcgctgatga agaaggcggg cggggcggac ttccacgacc atatcgtgcg gatctgcgag
     2881 atcgtcgacg gcgacgtttc ggccgaggtg acggcgaccg acttcgacgg catcatggcg
     2941 gaggcccaca cgctggccgc gctgcacccg caggtggtcg tgaaggtgcc gctcatcccg
     3001 gaaggcatcc gggccatccg gtcgctctcg ggcgaggaga tccgaaccaa ctgcacgctg
     3061 tgcttctcgc cgacccaggc gctcgtcgcg gccaaggcgg gcgccaccta catcagcccc
     3121 ttcgtcggga ggctcgacga catctcgacg tccggcatgg agctcatcgg ccagatcgtc
     3181 cagatctacg agaactacgg ctacgagacg caggtgctcg cggcgtcgat ccgccatcct
     3241 ctgcacgtgg tcgaggcggc gcttgccggt gccgacgtgg ccaccattcc gtactccgtg
     3301 ctgatgcagc tcatgaagca tcccctcacc gacctggggc tcgaacgctt cctggccgac
     3361 tgggagcagt accggcaggc ggccgcgacc tcgaacgggg cctcgtgagc gtccccacag
     3421 gtgagcggaa tgggtgaacg ggacatcggc ccggcgaccg cgtcgttcgt gcccctggga
     3481 ggaaccgacg agatcggggc gagctgccat ttcctgtcga tcgacgggac gggcatcctt
     3541 ctcgacgccg gggccgaccc ggagctcgag ggaacggcca gccttcccga cctggacctg
     3601 ctccggcgca ggacggaccg gtgggtggac cacgtgctgg tgtcgcacgc ccaccacgac
     3661 cacctcggat cgctccccgt cgtcatccgc gccttcccgc acgtcatggt ccacatgacc
     3721 agggccacgc gcgaccttgc cgacatcctg ctgccggcgt ccgccaagct gcagcgaagg
     3781 cgcctggtcg agggatcgtc gtccgcccag ccgctcttcg acaccgagga gctcggggcc
     3841 tattcctacc tgtacctcac gcacgagctg gagtcgccct tcgacgcgac gggcatccgg
     3901 ggggcgaccc ggatcgaggg ccggctgttc gacgccggtc acgtgctcgg atccgcgggc
     3961 atccagctca cggcccggac gccggacggg ccgcgcagcc tgttctacac gggcgacacg
     4021 agcctgcggc cgcagacggt gctccccggg gcgtcctacc ccgaagggcc cgtcgacacg
     4081 ctgatcatgg aggcgacgct cggcggcgat cccgacgccg aacagaccac ccgccgtcag
     4141 gaggagcggc ggctgggtga ggcccttcgc gagaccctgg atcgcggcgg gtgcgcggtg
     4201 ctcccggtct tcgcgctggg ccgcgggcag gagatcatcg cgctcctgga ccgcttccgg
     4261 aagaacggcg tcatcggcga ggacgtgccc atctattccg ccggcatgct gcgggcgatc
     4321 gcggacgtct acgaccgcac ccgcttctcc accccccgcg tggacaccga tttccaggtg
     4381 ttcggcgtcg agcagaagcg ccttccgagg ggcctcggag gcatggcgga ggcgctggcc
     4441 cgcccgggca ttttcgtcgt cgggagcggc atgatgttcg agcggacgct gtcgaaccgg
     4501 ctggcgcagg aggtcgtcga gcatgagcgg aacgccgtcc tcctggtcgg cttcgcgcgc
     4561 gaggactcgc ccgcgcatcg cctgatgcac gcggccggca acgggcaggc ctccgaggtc
     4621 gtcctggacg cgctgcgggg caagcagacc gtccggtgcc gcgtggagcg gttccgcttc
     4681 agcgggcaca gccaccggcg cgacctgatc gggctcgtcg agcgcatgcg gccgcggcgc
     4741 gtcatcatcg tgcacggcga gacgagggcc cgcgcctgga tggccgacaa catccggttc
     4801 ttccatccgg aggtcgacgt gtgcgtgccg gaccagggcc aggaagttcc gctctggccg
     4861 gatccggact gatcccggcc gttgcacccg gatcgttcga gagggatctt ggcggagtga
     4921 gcctcaacga tccttccgcg gtctcgagcg tgtccgatcc cacccctgcc cggcaacgga
     4981 tccgggagtt cgcagaatcc gtgcggggcc gcatcgccgg cgacctgcgc ctggacacca
     5041 tctcccgggc gctctacgcc acggacgcga gcatctacga gatcatgccg cttgcggtcc
     5101 tgctgccgcg cacgcccgac gacgtgcagg cggccctcct ggctgcccgc gcgcacggcg
     5161 tgccggtcct ggcgcgcggc ggcggttcca gcctggccgg gcagaccgtg aacgaggcgc
     5221 tcgtcatcga cttctcgcag cacctggacg cggtgctcga cctcgacgtc gaggccggac
     5281 ggatccgcgt gcagcccggc atcacgcacg agcggctggg gcgggccctg gcggggaccg
     5341 gctggatgat ggggccggac ccggcctcgg gcagccgcgc cacgctcggc ggcatgatgg
     5401 cgaacaacgc cacgggcacg cattccatcc tgtacggcaa cacgatccgg cacgtggagc
     5461 gggccgccgc cgtcctggcc gacggcacgc gcgtggactg gggcgcgatc gatgccgacg
     5521 cctggcgcgc ggccatccgg cggaccgacg gcgaagggcg cgtgctgcag ggcctggaca
     5581 tgatcgcgcg tggccgcggg gagatcatcc gcacccgcat gccccgccac tggcggcgca
     5641 acaacgggta ccgcgtcgag gagctgctcg agccgtccgt caaccccgcc cgcctcctgt
     5701 gcggcagcga ggggacgctg gcggtcgtca cggacctgac cgtgagcctg gtccggcgtc
     5761 ccgtcgcgac ggcggtcggc gtcgtgcact tcgcgacgcg cagggaggcg ctcgagtccg
     5821 tgacggcgat cctggagacg gatccctcgg ccgtcgagct cttcgacggc gccgcgatcg
     5881 ggcagtgccg ggcctcgcgc gggttcgccc accgcatgac cttcgtcgag ggcaaccccg
     5941 gggccatcct gctcaccgag tattacgggg aggcgcgcgc ggagctctcc gaccggctgg
     6001 accgcctcga gaagcgcatc ctcgggccga ccccggctac gggcatcgtc cgtctcgagg
     6061 atccgtccgc catcgagaac gcctggaccg tgcggcgcga ggccctgggc ctgatcatgt
     6121 ccgtccgcgg caacctgaag cccctgccct tcatcgagga tgcctcggtg ccggtcgagc
     6181 acctggccga ctacatctcg gacctggagg aggtcttcac cgagaccggg tcggaggccg
     6241 tcatgtacgc acatgcgtcg gcgggctgcc ttcacgtgcg gccgttcatc gacacgacgc
     6301 gggccgacga cgtcgcgcgc atggagcgga tcgcgtcggc ctccgccgat ctcgtgcgcc
     6361 ggtacgaggg ctgggtttcg tccgagcacg gggacgggct cacgcggagc tggctgaacc
     6421 cggtggtgct cggagaggag ctgtacgggg tgtgccgcga ggtcaagcac ctgttcgacc
     6481 cggagggcat cctgaacccg ggccgcatcg tggacgcgcc ccccatgacc ggatcgctcc
     6541 gcaacggacc ggactacgcg gcgatcccgg tcgagacgcg cctcgacttc gggcccgagg
     6601 gcggactggt ccgggccatc gagctctgca acggaaacgg ggcatgccgg cgcgtgggcg
     6661 ggggcaccat gtgcccgagc ttcatggcca cgcgcgagga gtcggactcc acgcgagggc
     6721 gtgcgaacgt gctgcgcatg gcgatggccg gccggacgga gggtgtcgac tggacggacc
     6781 cggccgtgta cgaggttctg gacctgtgcg tgcagtgcaa ggcctgcaag accgagtgtc
     6841 cttcggcggt gga
//
LOCUS       DBNHQZ010000220        11063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3588218, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000220 DBNHQZ010000000
VERSION     DBNHQZ010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3588218"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /gene="cimA"
                     /locus_tag="AC16YU_05890"
     CDS             complement(<1..1353)
                     /gene="cimA"
                     /locus_tag="AC16YU_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR6064787.1"
                     /translation="MARPTHDRTVEIFDTTLRDGTQGEHVSLSASDKMRIARRLDEAG
                     VDVIEGGWPGSNPKDQEFFDRAREHAWEHAEICAFGSTRRTGLAPESDPNLAAMLAAG
                     TPTVSLFGKSWTLHATVALGVSTDENLELIRSSVAYLKAAGRRVIYDAEHFFDGFAED
                     PAYALETLAAALDGGADVLVLCDTNGGTLPDAVSAAVREARARFNGTPLGIHTHNDSG
                     CAVANSLAAVAAGARHVQGTITGIGERCGNVDLCTVVPALQLKMGMECLPGLDRLFDL
                     AAFVSDVANLDPVDRAPYVGRSAFAHKGGVHVSAVMKDPRAYEHIDPALVGNRRRVLV
                     SDLSGRSNIRYKAAEFGIDLDDDAIAGSAVTRIKELENLGYAFEAAEASFELLLRQAR
                     GEEADYFHLERMSVRTEKGGPSGEHSEASLVLVVGEERNHVVAEGNGPVDAMSRAMRK
                     A"
     gene            complement(1357..1920)
                     /locus_tag="AC16YU_05895"
     CDS             complement(1357..1920)
                     /locus_tag="AC16YU_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR6064788.1"
                     /translation="MKAVIRGLAYVVGDAIDTDQIIPAAHLVYSMSEPEERRLYGRYA
                     LSAVPPEGQGLPGGGIPFTRSDAYRSDYTIVVAGRNFGCGSSREHAPFALMEAGCEAV
                     VAESYARIFYRNAVDAGFVVPFETRERLVDRVRTGDELEIDTQAATVRNLTTGEEWLL
                     NPLGDVADILRAGNVFEYARRNGLIAR"
     gene            complement(1917..3215)
                     /locus_tag="AC16YU_05900"
     CDS             complement(1917..3215)
                     /locus_tag="AC16YU_05900"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6064789.1"
                     /translation="MGMTLTEKILAAHAGRDRISPGENVWVDVDTLMTHDVCGPPTID
                     IFRREFGADARVWDKEKLVILPDHYIFTEDPHARRNIELLRAFAAEHALPHYYDVGTS
                     RYRGVCHVALAEEGYNRPGTVLFGTDSHTCTSGAFGLFSTGVGNTDAALIMGTGKIWV
                     RVPETMRFTFEGALPPYLMAKDLILAVIGDITTDGATYRAMEFDGEAVYDLSIEERMT
                     LTNMAIEAGGKNGIIAPDEKTMDYVRARTRVPFEAVYGDADARFHSERTYDVSTMEPV
                     IAKPHRPDNRATVSEVAGTRLDKCYIGSCTGGKLEDFEAAARILKGHEVRVATYVVPA
                     STQVQEDMKTRTVDGVTLWEIFEESGCLMGHASCGACLGGPIDTFGRATGTEVVISTT
                     NRNFPGRMGSKQSAVYLASPLTVAASALHGVITDPREVLV"
     gene            complement(3220..4854)
                     /locus_tag="AC16YU_05905"
     CDS             complement(3220..4854)
                     /locus_tag="AC16YU_05905"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR6064790.1"
                     /translation="MEATEHARGTDRVIIFDTTLRDGEQAPGAAMTVPEKVQIAHQLA
                     RLGVDIIEAGFPVSSPGQADAVRRIASEVQGPVVCGLARAVTGDIEAAAEALEGGERT
                     RIHTFIATSDIHLAAKFGDARYGATLEEKRRTVLRMAVDAVRLARTFTGDVEFSAEDA
                     GRTDIGFLAEIIDAAVEAGATTINIPDTTGYCVPREYGAMFEAVRERCALPDDVVLST
                     HCHDDLGLAVANSIAGLLAGARQVECTLNGIGERAGNASLEEIAMILQVRSDRFGLRT
                     GIDSRQLNPTSRLVSTFSGFAVQPNKAIVGRNAFSHEAGIHQDGVLKARETYEIMRAE
                     DVGQSVEAIRLGRHSGRHGLFSRMKRLGIHVSDADREAVYRRFERLADQKKEISDADL
                     HHLVDAPRATGEVPHFKLESASVAVGTHREPEAQVRIRHLRASRVTEGTGTGDGPIDA
                     LYRAIDHALDEHHDLVNYTIRSVTEGADALGEVAVLIDAGGPCFAGRASGTDVILASG
                     EAYVAALNALAAFRAEEESVRFVGDGIMRAFDPSEG"
     gene            complement(4866..5942)
                     /gene="leuB"
                     /locus_tag="AC16YU_05910"
     CDS             complement(4866..5942)
                     /gene="leuB"
                     /locus_tag="AC16YU_05910"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6064791.1"
                     /translation="MASYRIIVLSGDGIGPEVTGAALRVLEAAATRFGIEFDFERHPV
                     GGEAIDRFGDPLPGATLDACLASDGILLGAVGGPRWDAEPGSRRPEAGLLRLRRELGA
                     FANLRPSFVPDALVDASPLKAERVRGVDLVVVRELIGGIYYGEPRGRDAAGAFDTMRY
                     SVPEVERIARVAFLEARRRRNRVTSVDKANVLECSRLWRETVVRVHREEFPDVELGHL
                     YVDNAAMQIAVAPRQFDVILTGNLFGDILSDLSATLPGSLGLLPSASLGGTVGLFEPV
                     HGSAPDITGRGIANPVGCILCAAMLLDHLGEEGAAAAVRRAVDSALAAGLLTADLGGS
                     AGTLEVAGAVAAAVLAEEIPHTVS"
     gene            complement(5947..6960)
                     /gene="ilvC"
                     /locus_tag="AC16YU_05915"
     CDS             complement(5947..6960)
                     /gene="ilvC"
                     /locus_tag="AC16YU_05915"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6064792.1"
                     /translation="MKVHYEADPTLIRGRKVAVIGYGSQGHAHALNLHESGVDVAVGL
                     RPGSSNAAEAASKGLAVMSVPEAAAWGDVVMILIPDQHQKRVWESDIREHVTPGKALA
                     FGHGFNIHYGQIEPPEGVDVFMVAPKSPGHLVRRTYEEGKGVPCLVAVAADASGRALD
                     LALSYADAIGGTRAGVIETNFRDETETDLFGEQAVLCGGVAELIKGGFETLTEAGYPE
                     ELAYFECLHELKLIVDLFYEGGLSYMNYSVSDTAEYGGYSRGPRVVGPDAKAAMRRIL
                     DEVQSGSFAREWIAECEAGGARMDSLREANAGHPVEQVGRKLRAMMSWLRAKEPETTT
                     RQA"
     gene            complement(7009..7584)
                     /gene="ilvN"
                     /locus_tag="AC16YU_05920"
     CDS             complement(7009..7584)
                     /gene="ilvN"
                     /locus_tag="AC16YU_05920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR6064793.1"
                     /translation="MPEPQLTAQQIFRRKAAGEPLGPDDEAQVNRHVITVLLENSIGA
                     LNRVANLFSARGFNLESVSVGETDDAATSRMTIVTTGNDRIIGQVLQQLGNLVDTLVV
                     DDVTGEEHVERELCLLKVAYTDGTRAALMDVAEIFRGKIINITPETMMFEVTGPTTKI
                     NAFIGMMKPHGISEVARSGRVAMRRNLLFGS"
     gene            complement(7656..9434)
                     /gene="ilvB"
                     /locus_tag="AC16YU_05925"
     CDS             complement(7656..9434)
                     /gene="ilvB"
                     /locus_tag="AC16YU_05925"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR6064794.1"
                     /translation="MTTATASAIRTRPADPPRNGHREIPVLTGSEILIRSLEAEGVEV
                     VFGHPGGAVIRIYDDMHRMQPSFRHVLVRHEQGGTHAAEGYAKATGRVGTVLVTSGPG
                     ATNCVTGIADAYMDSVPIVVFTGQVPTHLIGNDAFQECDIIGVTRSITKHSFLVRDVR
                     DVAKTVKAAYHIAATGRPGPVVVDLPKDVLAASAPFEYPESVTLRGYDYVKEGDPDAV
                     REAAELIRTSKQPLLYVGGGTISSGATEVLTRLARKLRVPVTTTLHGLGSFPEDDPLS
                     VGMLGMHGTWYANMAVQHADLLIAVGARFDDRVTGKLEAWAPHAKVIHVDIDPACIAK
                     NVPVDCPVLGDVRLVLEALEPQVEPLDTAGWLARIDEWKKQCPLEWKDDGKLRPQAVI
                     TALREHTGGNAVVVTDVGQNQMWAAQFFRYARTRSHITSGGLGTMGFSLPASLGAAMG
                     LRGQDVPVISINGDGGFLMNSQELGVAAAQGLPIKIVVLNNGYLGMVRQWQELFHAER
                     YSHTDLRSTNPDFVKLAEAYGCVGLRCERPEDVRKTIEAAWEVTDRPVLMEFRVVTEE
                     MVFPMVPAGAATGDMITQRFNPELSA"
     gene            complement(9431..>11063)
                     /gene="ilvD"
                     /locus_tag="AC16YU_05930"
     CDS             complement(9431..>11063)
                     /gene="ilvD"
                     /locus_tag="AC16YU_05930"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6064795.1"
                     /translation="EADFQKPFIGICNSYIDLIPGHVHLQAFGRVVKEAVREAGGVPF
                     EFNTIGVDDGIAMGHLGMRYSLPSRELIADAVETVAMAHCLDGMVCIPNCDKIVPGML
                     MGALRVDIPTIFVSGGPMRAGRTPEGKKIDLISVFEGVGQFAQGTIGKEDLDVLEQFG
                     CPACGSCAGMFTANSMNCIMEALGLALPGNGSILAEDPRRHELARRAAARVVSLVAEN
                     LTPRQILTRDAFDDAFALDMAMGGSTNTVLHLLAAAVEAGIPYDLERINEVSERIPYL
                     CKVAPATPDVHMEDVDAAGGISAILAELHREGLLNADRPTVSGGTIADHAEAGENRDP
                     SIIHPVPAAFRRTGGLAVLFGNLAPRGAVVKTGAVAAHMQRFEGPARIFESQDAAAAG
                     ILGGEVEPGDVVVIRYEGPRGGPGMPEMLQPTSALAGMGLDTSVAMITDGRFSGGTRG
                     LSIGHCSPEAAAGGPIALVEPGDLVRIDLDARSIELVVDSAELDRRRSALAPFVPKIR
                     GGWLERYTHFVTSASEGAVLRVPEAIYAEPFTATP"
BASE COUNT         1569 a         3872 c         3947 g         1675 t
ORIGIN      
        1 cgcctttcgc atcgcgcgcg acatggcgtc gaccggcccg ttgccctcgg ccacgacgtg
       61 gttgcgctcc tcgcccacga ccagcaccag cgaggcctcc gagtgctcgc cggacgggcc
      121 gcccttctcg gtccgcacgc tcatgcgctc caggtggaag tagtcggcct cctccccgcg
      181 cgcctggcgg agcagcagct cgaacgaggc ctcggccgcc tcgaaggcgt agcccaggtt
      241 ctcgagctcc ttgatgcgcg tcacggcgga gcccgcgatc gcgtcgtcgt ccaggtcgat
      301 cccgaactcg gccgccttgt agcggatgtt cgagcggccc gagagatccg agaccaggac
      361 ccgccgccgg ttgccgacga gcgccgggtc gatgtgctcg tacgcccgcg ggtccttcat
      421 gaccgccgag acgtgcacgc cgcccttgtg cgcgaaggcg ctgcggccca cgtagggcgc
      481 ccggtcgacc gggtcgaggt tggccacgtc ggaaacgaag gccgccaggt cgaagagccg
      541 gtccagcccg ggcaggcact ccatgcccat cttgagctgc agcgcgggca cgaccgtgca
      601 caggtccacg ttgccgcacc gctcgccgat gcccgtgatc gtaccctgca cgtggcgtgc
      661 tccggccgcg acggcggcca gcgagttggc gaccgcgcag cccgagtcgt tgtgcgtgtg
      721 gatgccgagc ggcgtgccgt tgaaccgggc acgcgcttcg cggacggccg cggacaccgc
      781 gtcgggaagc gtgcccccat tcgtgtcgca gagcacgagg acgtcggcgc cgccatcgag
      841 ggcggccgcc agggtctcca gggcgtaggc cgggtcctcc gcgaagccgt cgaagaagtg
      901 ctccgcgtcg tagatcaccc gccggccggc cgccttcagg tacgcgaccg acgagcggat
      961 gagctccagg ttctcgtcgg tcgacacccc gagcgcgacc gttgcgtgca gcgtccagct
     1021 cttgccgaag agcgacacgg tgggcgtacc cgcggcgagc atggcggcca ggttcgggtc
     1081 ggactccggc gcaagaccgg tgcggcgcgt cgagccgaag gcgcagatct ccgcgtgctc
     1141 ccacgcgtgc tcccgcgccc ggtcgaagaa ttcctggtcc ttgggattgg agccgggcca
     1201 cccgccctcg atcacgtcca cgccggcctc gtccagccgc cgggcgatcc gcatcttgtc
     1261 ggaggccgag agcgagacgt gctcgccctg ggttccgtcc cggagcgtgg tgtcgaagat
     1321 ctcgaccgtg cggtcgtggg tggggcgggc catggctcag cgggcgatga gtccgttccg
     1381 gcgcgcgtac tcgaagacgt tgcccgcccg caggatgtcg gccacgtcac cgagcgggtt
     1441 caggagccat tcctcgccgg tcgtgaggtt gcggacggtg gcggcctgcg tgtcgatctc
     1501 gagctcgtcg cccgtccgca cgcggtccac gaggcgctcg cgcgtctcga aggggacgac
     1561 gaagccggcg tccacggcgt tgcggtagaa gatgcgggca tacgactcgg cgaccaccgc
     1621 ctcgcagccg gcctccatga gcgcgaaggg agcatgctcc cgtgacgagc cacacccgaa
     1681 gttgcgcccg gcgaccacga tcgtgtagtc ggatcggtac gcgtcggacc gggtgaaggg
     1741 gatgcctccg ccgggcaggc cctggccctc gggcggcacg gcgctcagcg cgtagcggcc
     1801 atagaggcgc cgctcctccg gctcggacat cgagtagacg aggtgcgcgg ccgggatgat
     1861 ctggtcggtg tctatggcat cgcccacgac gtaggcgagt ccccggatga cggctttcat
     1921 accagcacct cgcgaggatc ggtgatgacg ccgtggagcg ccgaggccgc gaccgtgagc
     1981 ggggacgcga ggtacaccgc cgactgcttc gagcccatgc ggcccgggaa attgcggttg
     2041 gtcgtcgaga tcacgacctc ggtgccggtc gcgcgcccga acgtgtcgat cggcccgccc
     2101 aggcaggcgc cgcatgaggc gtggcccatg aggcagcccg actcctcgaa gatctcccaa
     2161 agcgtcacgc cgtcgacggt cctcgtcttc atgtcctcct ggacctgcgt ggaggcgggg
     2221 acgacgtacg tcgcgaccct cacctcgtgc cccttcagga tccgcgcggc cgcctcgaaa
     2281 tcctcgagct tgccgcccgt gcaggagccg atgtagcact tgtccaggcg cgttccggcg
     2341 acctccgaga ccgtcgcgcg gttgtcgggc cggtgcggct tcgcgatgac gggctccatc
     2401 gtcgatacgt cgtaggtgcg ctcggagtgg aagcgggcgt ccgcgtcgcc gtagacggcc
     2461 tcgaacggga cacgggtgcg cgcccgcacg tagtccatcg tcttctcgtc gggcgcgatg
     2521 atgccgttct tgccgcccgc ctcgatcgcc atgttcgtga gcgtcatccg ctcctcgatc
     2581 gagaggtcgt acacggcctc cccgtcgaat tccatggcgc ggtaggtggc gccgtcggtc
     2641 gtgatgtcgc cgatgacggc caggatcagg tccttggcca tgaggtacgg cggcagcgcg
     2701 ccctcgaacg tgaagcgcat ggtctcgggc acgcgaaccc agatcttccc cgtgcccatg
     2761 atgagcgcgg cgtccgtgtt gccgacgccc gtcgagaaga ggccgaaggc acccgacgtg
     2821 cacgtgtgcg agtccgtgcc gaagagcacg gtgccgggcc ggttgtaccc ctcctccgcg
     2881 agcgccacgt ggcagacacc cctgtagcgc gaggtgccca cgtcgtagta atgcggcagg
     2941 gcgtgctcgg ccgcgaaggc gcgcaggagc tcgatgttgc ggcgggcgtg cgggtcctcg
     3001 gtgaagatgt agtggtcggg aaggatgacg agcttctcct tgtcccacac cctcgcgtcc
     3061 gccccgaact cgcgcctgaa gatgtcgatg gtcggcgggc cgcagacgtc gtgcgtcatg
     3121 agcgtgtcca cgtccaccca gacgttctcg ccgggcgaga tgcggtcgcg gccggcgtgg
     3181 gccgcgagga tcttctcggt gagagtcatg cccatcgggt cagccctccg acgggtcgaa
     3241 ggcccgcatg atgccgtcgc ccacgaagcg gaccgactcc tcctcggccc ggaaggcggc
     3301 gagcgcgttc agggcggcga cgtacgcctc gcccgaggcc aggatcacgt cggtgcccga
     3361 ggcgcgcccg gcgaagcagg gcccgcccgc gtcgatgagg acggccacct cgccgagcgc
     3421 gtcggcgccc tcggtgaccg agcggatcgt gtagttcacc aggtcgtggt gctcgtccag
     3481 cgcgtggtcg atggcccggt acagggcatc gatcgggccg tcgcccgtcc ccgtgccctc
     3541 ggtcacccgg ctcgcccgca ggtgccggat gcggacctgc gcctcgggct cgcggtgcgt
     3601 gccgacggcg acggaggccg actcgagctt gaagtggggc acctcgccgg tcgcgcgcgg
     3661 ggcgtccacg aggtggtgca ggtcggcatc cgagatttcc ttcttctggt cggcgagacg
     3721 ctcgaatcgg cggtacaccg cctcgcggtc cgcgtcggac acgtggatgc cgagccgctt
     3781 catccgcgag aacaggccgt ggcggcccga gtggcggccg agccggatcg cctcgaccga
     3841 ctggccgacg tcctcggccc gcatgatctc gtacgtttcc ctggccttga ggacgccgtc
     3901 ctggtggatg cccgcctcgt ggctgaatgc gttgcgcccg acgatggcct tgttcggctg
     3961 cacggcgaaa cccgagaagg tcgacaccag gcggctcgtc gggttcagct gacgcgagtc
     4021 gatgccggtc cgcagcccga agcggtccga gcggacctgc aggatcatgg cgatctcctc
     4081 gagcgacgcg ttgcccgccc gctcgccgat gccgttgagc gtgcactcga cctgccgcgc
     4141 ccccgcgagg aggcccgcga tggagttcgc gacggcgagc cccaggtcgt cgtgacagtg
     4201 ggtcgagagc acgacgtcgt cgggcagcgc gcagcgctcg cggaccgcct cgaacatggc
     4261 cccgtactcg cgcggcacgc agtagccggt cgtgtcggga atgttgatgg tggtcgcgcc
     4321 cgcctcgacg gccgcgtcga tgatctcggc caggaagccg atgtccgtcc ggcccgcgtc
     4381 ctcggccgag aactcgacat cgccggtgaa cgtgcgggcg agccggaccg cgtcgacggc
     4441 catccggagc accgtgcggc gcttctcctc gagggtcgcg ccgtagcgcg catcgccgaa
     4501 cttcgcggcg aggtggatgt cgctcgtggc gatgaaggtg tggatccgcg tccgctcgcc
     4561 cccctccagc gcctcggccg ccgcctcgat gtcgcccgtg accgcgcggg cgagcccgca
     4621 gaccaccggg ccctgcacct cggacgcgat ccggcggacc gcatcggcct ggcccggcga
     4681 cgagaccgga aagccggcct cgatgatgtc gacgccgagg cgggcgagct ggtgcgcgat
     4741 ctgcaccttt tcgggaacgg tcatcgccgc cccaggggcc tgctcgccgt cgcgcagcgt
     4801 ggtgtcgaag atgatgaccc ggtcggtgcc ccgggcgtgt tctgtggctt ccatcgaacg
     4861 gtggatcagg agacggtgtg tggaatttct tcggcaagga cggccgcggc gaccgcaccc
     4921 gcgacctcca gggtcccggc gctgccgccc aggtcggcgg tgaggagccc ggccgccagc
     4981 gcgctgtcga cggcgcggcg gaccgcggca gcggcgccct cctcgcccag gtggtcgagc
     5041 agcatggccg cgcacaggat gcagccgacc ggattggcga tgccccggcc cgtgatgtcg
     5101 ggggccgagc cgtgcacggg ctcgaagagg cccaccgtgc cgcccaggct ggccgacggc
     5161 aacaggccca gcgaccccgg gagcgtggcc gagaggtccg agaggatgtc gccgaacagg
     5221 ttgccggtca ggatgacgtc gaactgccgc ggggcgacgg cgatctgcat cgcggcgttg
     5281 tccacgtaca ggtgaccgag ctccacgtcc gggaactcct cccggtgcac gcggaccacg
     5341 gtctcgcgcc acaggcggga gcactcgagc acgttggcct tgtcgaccga cgtcacccgg
     5401 ttccggcggc ggcgcgcctc gaggaaggcg acgcgcgcga tgcgctcgac ctcgggcacg
     5461 gaatagcgca tcgtgtcgaa ggcgccggcc gcgtcgcgcc cgcgcggctc gccgtagtag
     5521 atgccgccga tgagctcgcg caccacgacc aggtccaccc cgcgcacccg ctccgccttc
     5581 agcggcgagg catcgacgag ggcgtccggc acgaaggacg gccggaggtt ggcgaaggcg
     5641 cccagctcgc gccgaaggcg gagcaggccc gcctcgggcc gccggctccc cggctccgcg
     5701 tcccacctgg gcccgcccac ggccccgagc aggatgccgt cggacgcgag gcaggcgtcg
     5761 agcgtcgccc cgggcagcgg gtcgccgaac cggtcgatgg cctcgcctcc gaccggatgc
     5821 cgctcgaagt cgaattcgat gccgaagcgc gtggccgccg cctccagcac gcggagcgct
     5881 gcgcccgtga cctcgggacc gatgccgtcg ccggagagga cgatgatgcg atacgatgcc
     5941 atggaatcag gcctgccgcg tggtggtctc gggctccttc gcccgcagcc aggacatcat
     6001 ggcgcgcagc ttccggccca cctgctcgac gggatgcccg gcattcgcct cgcgcagcga
     6061 atccatgcgc gcgccgcccg cctcgcactc ggcgatccac tcgcgcgcga agctgccgga
     6121 ctgcacctcg tccaggatcc ggcgcatggc ggccttcgcg tccggcccca cgacccgcgg
     6181 gccgcggctg tagccgccgt actcggccgt gtcgctcacg gagtagttca tgtaggagag
     6241 cccgccctcg tagaagaggt ccacgatgag cttcagctcg tgcaggcact cgaagtaggc
     6301 gagttcctcg gggtagccgg cctcggtgag cgtctcgaac ccgcccttga tgagctcggc
     6361 gacgccgccg cagagcacgg cctgctcgcc gaagaggtcg gtctcggtct cgtcgcggaa
     6421 attggtctcg atgacgcccg cgcgcgtgcc gccgatcgcg tcggcgtagg agagggcgag
     6481 gtccagggcc cggcccgagg cgtcggccgc gaccgccacg aggcacggca cgcccttccc
     6541 ctcctcgtac gtgcggcgga ccaggtggcc gggagacttg ggcgcgacca tgaagacgtc
     6601 gacgccctcg ggcggctcga tctggccgta atggatattg aagccgtgcc cgaaggcgag
     6661 cgccttgccg ggcgtcacgt gctcgcggat gtccgactcc cacacccgct tctggtgctg
     6721 gtccgggatc aggatcatga cgacgtcgcc ccaggccgcg gcctcgggga ccgacatcac
     6781 ggcgaggccc ttcgaggcgg cctcggccgc gttcgacgag ccggggcgca gccccacggc
     6841 cacatccacc ccgctctcgt gcaggttcag ggcgtgggcg tggccctggc tgccgtatcc
     6901 gatgacggcc accttgcggc cgcggatgag ggtggggtct gcttcgtaat ggactttcat
     6961 ggtgatgcgg atggatgggg gcaggaatcg gccgcgccgg gacggggctc aggatccgaa
     7021 cagcaggttt cggcgcatcg cgacccggcc cgagcgggcc acctcgctga tgccgtgcgg
     7081 tttcatcatc ccgatgaagg cattgatctt ggtcgtggga cccgtgacct cgaacatcat
     7141 ggtctcgggc gtgatgttga tgatcttccc gcggaagatc tcggcgacgt ccatgagcgc
     7201 ggctcgcgtc ccgtccgtgt aggccacttt caagagacac agctcgcgct cgacgtgctc
     7261 ctcgccggtc acgtcgtcca cgacgagcgt gtccaccagg ttgcccagct gctggagcac
     7321 ctggccgatg atgcggtcgt tgccggtcgt gacgatggtc atgcgcgagg tcgccgcgtc
     7381 gtccgtctcc ccgaccgaca cggactccag gttgaatccg cgcgccgaga acaggttggc
     7441 gacgcggttc agcgcgccga tcgagttctc gaggagcacc gtgatcacgt ggcggttcac
     7501 ctgggcctcg tcgtcggggc cgaggggctc tccggccgcc ttgcgccgga agatctgctg
     7561 ggcggtgagc tggggttcgg gcatggtcgt gcggtcgggc cgggttggag cgcctcggaa
     7621 gtcaggccgg gagctcgggg ttggggcgcc tcgggtcagg ccgagagctc ggggttgaag
     7681 cgctgggtga tcatgtcgcc ggtcgccgcg ccggcgggca ccatcgggaa gaccatctcc
     7741 tcggtcacga cgcggaattc catgagcacg ggccggtcgg taacctccca agccgcctcg
     7801 atggtcttcc ggacgtcctc gggccgctcg caccggagcc ccacgcatcc gtaggcctcg
     7861 gcgagcttca cgaagtcggg gttcgtggac cgcaggtcgg tgtgcgagta gcgctccgcg
     7921 tggaagagct cctgccactg ccgcaccatg cccaggtagc cgttgttcag caccacgatc
     7981 ttgatcggga ggccctgcgc ggccgccacg cccagctcct ggctgttcat gagaaagccg
     8041 ccgtcgccgt tgatcgatat gacgggcacg tcctggcccc gcaggcccat cgcagcgccg
     8101 agagaggccg gcagcgagaa acccatcgtg cccaggccgc cggacgtgat gtgcgagcgc
     8161 gtccgcgcgt agcggaagaa ctgcgcggcc cacatctgat tctgtccgac gtcggtgacg
     8221 acgaccgcgt tgccgcccgt gtgctcgcga agagcggtga tgacggcctg cggccgcagc
     8281 ttgccgtcgt ccttccactc gagcggacac tgcttcttcc actcgtcgat ccgggcgagc
     8341 cagccggccg tgtcgagcgg ctcgacctgc ggctcgagcg cctcgagcac cagccgcacg
     8401 tcgccgagca cggggcagtc gacgggcacg ttcttggcga tgcatgccgg gtcgatgtcg
     8461 acgtggatca ccttcgcgtg cggcgcccag gcctcgagct tgccggtgac ccggtcgtcg
     8521 aagcgcgccc cgacggcgat gagcaggtcg gcgtgctgca cggccatgtt cgcataccac
     8581 gtgccgtgca tgcccagcat gcccaccgac agcgggtcgt cctcggggaa ggagccgagc
     8641 ccgtgcagcg tggtggtgac cggcacgcgg agcttccgcg cgagccgcgt caggacctcg
     8701 gtggcgcccg acgagatcgt gccgcccccg acatagagga gcggctgctt cgacgtacgg
     8761 atcagctccg ccgcctcgcg gacggcgtcc gggtcgccct ccttcacgta gtcgtagccg
     8821 cgcagcgtca ccgactcggg atactcgaag ggcgcgctcg cggcgagcac gtccttgggc
     8881 aggtccacga ccacggggcc cggccgcccg gtggccgcga tgtggtaggc cgccttcacg
     8941 gtcttcgcca cgtcgcggac gtcgcggacc aggaagctgt gcttggtgat ggagcgcgtc
     9001 accccgatga tgtcgcactc ctggaaggcg tcgttgccga tcaggtgcgt cgggacctgc
     9061 cccgtgaaca ccacgatcgg caccgagtcc atgtaggcgt ccgcgatgcc cgtgacgcag
     9121 ttcgtggcgc ccggtccgct cgtaaccaac accgtaccga cacgcccggt ggccttggca
     9181 tacccttcgg ccgcgtgcgt gccgccctgc tcgtggcgga ccagcacgtg acggaacgac
     9241 ggctgcatgc ggtgcatgtc gtcgtagatg cggatgaccg cgccgccggg gtggccgaag
     9301 accacctcca ccccctcggc ctcgagcgag cggatcagga tctccgaccc ggtgagcacg
     9361 ggaatctcgc gatggccgtt gcgcggcgga tcggccggac gggtccggat ggcggatgcg
     9421 gtggcggtcg tcatggtgtg gcggtgaaag gttcggcgta aatggcctcc gggacgcgga
     9481 ggacggcgcc ctcggaggcg ctggtgacga aatgcgtgta gcgctccagc catccgcccc
     9541 ggatcttcgg gacgaaggga gcgagcgcgg agcggcggcg gtcgagctcc gccgagtcca
     9601 cgacgagctc gatggaccgc gcgtccaggt cgatacggac caggtccccc ggctcgacga
     9661 gtgctatggg gcctcccgcg gcggcttccg gcgagcagtg cccgatcgac aggccgcgcg
     9721 tcccccccga gaagcggccg tcggtgatca tggctacgct cgtatccagg cccatgccgg
     9781 ccagcgccga agtcggctgg agcatctcgg gcatgccggg gccgccgcgg gggccctcat
     9841 atcgaatgac gacgacgtcg cccggctcga cctctccgcc caggatcccg gccgccgccg
     9901 cgtcctggct ttcgaagatg cgggccgggc cctcgaaccg ctgcatgtgc gcggccacag
     9961 cgcccgtctt gacgacggcg ccccggggcg ccaggttgcc gaagagaacc gccaggccgc
    10021 ccgtcctgcg gaaggccgcc gggaccgggt ggatgatcga cggatcgcgg ttctcgcccg
    10081 cctcggcgtg gtcggcgatc gtgccgcccg agaccgtcgg ccggtcggcg ttcaggaggc
    10141 cctcgcggtg cagctcggcg aggatcgcgg agatgccgcc ggccgcgtcc acgtcctcca
    10201 tgtggacgtc gggcgtcgcg ggcgccacct tgcacaggta cgggatccgt tcggagacct
    10261 cgttgatccg ctccaggtcg tacgggatcc ccgcctcgac cgccgctgcc aggaggtgaa
    10321 ggacggtgtt ggtcgatcca cccatcgcca tgtccagcgc aaaggcgtcg tcgaaggcgt
    10381 cccgcgtcag gatctgccgg ggcgtcaggt tctcggccac gagactcacc acgcgggcgg
    10441 cggcccgccg cgcgagctcg tgtcggcgcg ggtcctcggc gaggatcgag ccgttgccgg
    10501 gcagagcgag cccgagggcc tccatgatgc agttcatgga gttcgccgtg aacatgcccg
    10561 cgcacgagcc gcaggccggg cagccgaact gctcgaggac gtcgaggtcc tccttgccga
    10621 tcgtgccctg cgcgaactgc cccacgccct cgaagaccga gatgaggtcg atcttcttcc
    10681 cttccggcgt ccggccggcg cgcatcgggc cgcccgacac gaagatcgtc gggatgtcga
    10741 cgcgcagcgc gcccatcagc atgcccggga cgatcttgtc gcagttaggg atgcagacca
    10801 tgccgtccag gcagtgcgcc atggcgaccg tctccacggc gtccgcgatg agctcgcgcg
    10861 agggcagcga gtagcgcatg cccaggtggc ccatcgcgat gccgtcatcg accccgatcg
    10921 tgttgaactc gaacggcacg ccgcccgcct cgcgcacggc ctccttcacc acgcgcccga
    10981 acgcctgcag gtgcacgtgc cccgggatca ggtcgatgta cgagttgcag atcccgatga
    11041 agggcttctg gaagtccgcc tcg
//
LOCUS       DBNHQZ010000221         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_65606, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000221 DBNHQZ010000000
VERSION     DBNHQZ010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_65606"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16YU_05935"
     CDS             <1..283
                     /locus_tag="AC16YU_05935"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6064796.1"
                     /translation="PTGPALGLVPGADFTVDHTRLRPGDTAVAFTDGATEARNPEGGF
                     FTEERLLPLLSTRAATASEVLDRVERAVGAFTGDAAASDDLTMLAVRRV"
     gene            complement(317..472)
                     /locus_tag="AC16YU_05940"
     CDS             complement(317..472)
                     /locus_tag="AC16YU_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064797.1"
                     /translation="MLDMPYRLFDAPTSTAVKLGIVVLAVVLYGLVWIAVRGVYSRLG
                     GAVGGTN"
     gene            complement(515..715)
                     /locus_tag="AC16YU_05945"
     CDS             complement(515..715)
                     /locus_tag="AC16YU_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064798.1"
                     /translation="MLRRFVSAASVGAAVVGLAGPLLYMNPLPTPEGRYIVATVWCMV
                     PLAWGIWAMLLPATWVPGRIPL"
     gene            900..1289
                     /locus_tag="AC16YU_05950"
     CDS             900..1289
                     /locus_tag="AC16YU_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR6064799.1"
                     /translation="MPGPLPHTLSRRERQIMDVLYRMESASAAEIRAQMPDAPSYSAV
                     RAHLRILEEKGHVRHETQGPRYVYRPTVPASDARRSALRHVVRTFFEGSTTQAVAALL
                     GDSPVDLEDEEYERLLALIERARQEGR"
     gene            1294..3852
                     /locus_tag="AC16YU_05955"
     CDS             1294..3852
                     /locus_tag="AC16YU_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR6064800.1"
                     /translation="MMSDWNILEPAWANGVLGALADHALRSSVLLVGALLVTAALRRR
                     AASVRHAIWMSVFAAVLLLPVGTWLLPDWEVLPSRSPVVSETASAAETEAFPLQRSAR
                     RPAMQSAAGSTAAGRESAGSRATPDPQPTPWVVARSVRDLAARANEVTPARWLVLAWL
                     AGLVLGLARQANGAIQMRRITSAGVRPSDPVLALADRALARLGMRQAVDIRLSPRVPM
                     PATRGRRSAAILLPISARHWDEDRLWHVLLHELAHVARRDHTVQTLARAVCSVFWFNP
                     LLWLALRSLRAESEIASDDRVVLSGSTASRYAGHLVAVARSVRATSPRLPAAALAMAH
                     PSRLEDRIRTILDPRTSRIPMKPHHALLSMAAVLLVSLPVAAAARQVIPPPRPSPSPP
                     APVAVPGTPAPVPSAIPAAPDPAAAPAPAPAPSPMARPVPSPAARPAVPAPAPEAQPA
                     VPFHAPRPIAPPDTLKNRARVIAAFIAALQDPDPEMRRQAAWGLAELDASEAVEPLAG
                     VLADDQDPEVRRTALHALGELDPDAALPAITSRFRSETEPETRREMLWVMAESGEASM
                     LPAVQSALDDGDPGVRRDALMALAELGTPETVPLLTGALDDPDPDIQRMALHALAELA
                     GPADVSQAALAAVVRATKSADPDVREAAIVALAEAGGDAAVPALVAATTDSEPDVRRH
                     ALWALAESDSPDAIPALARAASDADPEVRRFALYALAERDGAASFGTLVDALDDPDSE
                     VRRAAVTALAESEHPEAADALARVLEDEDTDVRQMAIWGLAESGSADAAVTARLVAVL
                     EGDAEPEVRQAAVHALGEIGSDAALDALTRALQDPDPDVRKAAMAALADLAGGR"
     gene            complement(3918..>4364)
                     /locus_tag="AC16YU_05960"
     CDS             complement(3918..>4364)
                     /locus_tag="AC16YU_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064801.1"
                     /translation="GETIELTDRRTANRRYPEFSPDGTTIAYEQDGQTFTVPADGRGV
                     PVNRTTGLWGQPSWSADGRKIYGLGSTGLVSREIETAAVDFELGFTWSVPHFYDVYSS
                     GERVLMTSPGLAAGSGVGPEGAVPSDSTESLVFIINFDEALRGQSR"
BASE COUNT          614 a         1628 c         1501 g          621 t
ORIGIN      
        1 cccgaccggg ccggccctgg gcctggtgcc gggcgctgac ttcacggtcg accacacccg
       61 gctgcgcccc ggcgacacgg cggtcgcgtt cacggacggc gcgaccgagg cccgcaaccc
      121 cgaagggggg ttcttcaccg aggagcggct gctgccgctg ctttcgaccc gggccgcgac
      181 ggcatccgag gtgctggacc gcgtcgagcg ggcggtcggg gcctttaccg gcgatgccgc
      241 ggcatcggat gacctgacga tgctggcggt gcggcgggtg taagggttgg ggacggttcg
      301 atccggcccg cccccgctaa ttcgttcccc ccactgcccc gcccagcctc gagtacaccc
      361 cgcgcaccgc gatccacacg agtccgtaca ggactacggc cagaacgacg atcccgagct
      421 tcacggcagt cgaggtcggc gcgtcgaaca gccggtaggg catgtcgagc acgaaggcgg
      481 caatgatgcc ggccaggaca ccgagaagcg cgcctcagag cgggatgcgc cccggcaccc
      541 aggtggccgg caaaagcatg gcccagatgc cccaggccag gggaaccatg caccacacgg
      601 tcgccacgat atagcgtccc tcgggagtcg ggagcgggtt catgtacaga aggggaccgg
      661 ccaacccgac cactgcggcc ccgaccgagg ccgccgatac gaagcgtcga agcatgacat
      721 acctcatggt cttgccgcgc gcggacaagg tacgaccgga gcccgggcga cgccgcccgg
      781 catggggtaa gaaattagca cctcgcttga ccctgcggcg aaaggcgctt acgtttcagg
      841 tggtaatatt ttagcacagc cgcaaccggg gcctcccctg ccccgtaccg cgcttcctca
      901 tgccgggacc tcttccgcac accctgagcc gccgggaacg ccagatcatg gacgttctct
      961 accggatgga gtccgcctcg gcggccgaaa tccgtgccca gatgcctgac gcgccgagct
     1021 actcggccgt ccgcgcccac cttcgcatac tcgaggagaa gggccacgtg cgacacgaaa
     1081 cgcagggacc gcgctacgtg taccgtccca ccgttccggc cagcgacgcc cgccgatccg
     1141 ctctgcgcca cgtcgtccga acgttcttcg agggctccac gacgcaggcg gtcgcggccc
     1201 tgctcggcga ttcgccggta gatctggagg acgaggagta cgaacgtctc ctcgccctca
     1261 tcgaacgggc acggcaggag ggccgctgac accatgatgt ccgactggaa catcctcgag
     1321 ccggcctggg cgaacggggt gcttggagcg ctcgcggatc atgccctgcg atcatccgtc
     1381 ctgctggtcg gcgcgctcct cgtcacggcc gctcttcgac gccgcgcggc gtcggtcagg
     1441 cacgcgatct ggatgtcggt tttcgcagcc gtcctcctgc tccccgtggg aacgtggctc
     1501 ctgcccgact gggaggttct cccctcccgg tcgccggtcg tctccgagac ggcgtcagcc
     1561 gcggagaccg aagccttccc cctgcagcgc agcgcgcgcc ggcccgccat gcagtcggcg
     1621 gccggatcga cggccgcagg gcgggagtcg gccggttcgc gggcaacccc ggacccgcag
     1681 ccgacaccgt gggtcgtcgc ccgttcggtc cgcgacctcg ccgcccgggc caacgaggtc
     1741 actccggcgc ggtggctcgt gctggcgtgg ctggccgggc tcgtgctggg cctcgcacga
     1801 caggcgaacg gcgcgatcca gatgcgaagg atcacgagcg cgggcgtccg cccgtccgac
     1861 ccggtactcg cgctcgcgga tcgcgcgctc gcccgactgg gcatgcggca ggcggtcgac
     1921 atccgcctga gcccccgcgt gccgatgccc gcgacccggg gcagacgaag cgccgcgatc
     1981 ctgctcccga tctcggccag gcactgggac gaagaccggc tctggcacgt cctcctgcac
     2041 gagctcgccc acgtggcgcg ccgcgatcac accgtgcaga cgctggcccg ggcggtctgc
     2101 agcgtcttct ggttcaaccc gctcctgtgg ctcgccctgc gaagccttcg tgccgagagc
     2161 gagatcgcct ccgacgaccg cgtcgtgctc tccggcagca cggcgtcccg ttacgccggt
     2221 cacctggtcg cggtggcccg ctccgtgcgg gccacgtcgc cgagactgcc cgccgcggcg
     2281 ctcgccatgg cgcatccctc ccgtctggaa gaccgcatcc gcaccatcct ggatccccga
     2341 acttctcgca tcccgatgaa acctcaccat gcactgctgt cgatggcggc ggtcctcctc
     2401 gtttccctgc ctgtcgccgc cgccgcacgc caggtgatcc cgccgccccg gccttcgccg
     2461 tccccgccgg cgcccgtcgc ggtccccggg acacccgctc cggtgccatc tgcaatcccg
     2521 gcggcgcccg atccggcagc cgctccggca cccgctccgg cgccgtcgcc catggctcgg
     2581 ccagtcccgt cgccggcggc gcggcccgcc gtgccggctc ccgcgccgga ggcgcagccc
     2641 gccgtgccat tccacgcgcc gcggccgatc gcccccccgg acacgttgaa gaaccgggcg
     2701 cgcgtgatcg ccgcgttcat cgcggcactg caggatcctg atcccgaaat gcggcggcaa
     2761 gcggcctggg ggctggccga gctggacgcg tccgaggcgg tcgaaccgct ggccggggtg
     2821 cttgccgatg accaggatcc cgaggtgcga aggacagccc tgcacgcgct cggcgagctg
     2881 gaccctgacg ccgcgctgcc ggccatcacg agccggttcc ggtcggagac ggagcccgag
     2941 acccggcgcg agatgctctg ggtcatggcg gagtccggcg aggcgtcgat gcttcccgcc
     3001 gtgcagtccg cgctcgacga cggtgacccg ggcgtacgga gggacgcgct catggccctt
     3061 gccgaacttg gtacccccga gaccgtcccg ctcctgacgg gggcgctcga cgatccggat
     3121 cccgacatcc agcggatggc cctgcacgcg ctcgcagagc tggccgggcc ggctgacgtg
     3181 tcgcaggcag cgctcgccgc ggtggtccga gccacgaaga gcgccgatcc cgacgtgcgt
     3241 gaggctgcca tcgtcgccct cgccgaagcc ggtggcgatg cggccgttcc cgccctcgtc
     3301 gccgccacga ccgactcgga gccggacgtg cgccggcacg cgctatgggc cctggccgaa
     3361 tcggactccc cggacgccat tccggccctg gcccgcgcgg cgtccgacgc cgatcccgag
     3421 gtccggcgct tcgcgctgta cgcgctggcc gagcgtgacg gcgccgcgtc gttcggcacc
     3481 ctggtcgacg cgctggacga tccggattcc gaagtgcgcc gtgcggcggt aacggccctg
     3541 gccgagtcgg agcaccccga ggcggccgac gccctggccc gcgtcctgga ggatgaggac
     3601 accgacgtcc gccagatggc gatctggggg ctggccgaga gcggatcggc cgacgccgcg
     3661 gtgaccgctc gcctcgtcgc cgtgctggaa ggcgatgccg aacccgaggt ccgtcaggcg
     3721 gccgtgcacg ccctgggcga gatcgggagt gacgcggcgc tcgacgcgct gacccgcgcc
     3781 cttcaggatc cggatccgga tgtgcgcaag gcggcaatgg cggcgctggc ggatctggcc
     3841 ggcggtcggt aaccggcggc gggcggcaac cggccggcag gcaatggctg gccggcggtc
     3901 gattgcggac gcgccggcta ccgagactgc ccccgaagtg cctcgtcgaa gttgatgatg
     3961 aagacgagcg actcggtgga atccgacggg acggctcctt ccggcccgac tccacttccg
     4021 gccgccaggc ccgggctggt catcagcacc ctttcgccgc tcgagtagac gtcgtagaag
     4081 tggggcacgg accaggtgaa gcccagttcg aagtcgacgg cggcggtttc gatttcgcga
     4141 ctgaccagcc ccgtcgagcc caggccgtaa atcttccgtc cgtcggccga ccatgacggt
     4201 tgaccccaga gccccgtcgt gcggttgacc ggtacgcccc gtccgtcggc gggcacggta
     4261 aacgtctggc cgtcctgctc gtacgcgatc gtcgtgccgt cgggcgaaaa ctcggggtag
     4321 cgccggttcg cggtccgccg gtcggtaagc tcgattgttt cccc
//
LOCUS       DBNHQZ010000222         7095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3452640, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000222 DBNHQZ010000000
VERSION     DBNHQZ010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3452640"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="AC16YU_05965"
     CDS             <1..1798
                     /locus_tag="AC16YU_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR6064802.1"
                     /translation="PAHSPSDPGEFVPRWAKSAVWYQIFPERFRNGDPSNDPTVRDIA
                     GADPQEPPKVWHVHPWGSDWYRLQSWEMENGEPELWKHLLRRRYGGDLQGIIDRLDYV
                     DSLGVTALYLNPVFDSPSLHKYDGASYHHVDPNFGPDPDGDRAMIAEEDPLDPSTWVW
                     TSADRLALELVGEVHRRGMRIIFDGVFNHMGRNSFAFRDLAEKQQDSPYADWFVVRSW
                     DDPAAGTRFEYSGWAGVRSLPEFREDENGIVEGPSAYIFAATERWMNPDGRGTAHGID
                     GWRLDVAYDVSHAFWKRWRSHVRWINPEAYLTAELVAPPERVVPYLEGDEFDGEMNYN
                     FAFTAAEFLFNPEPMRITPTAFDRELERLRTMYPAGVAYVMQNLFGSHDSNRIGSHIV
                     NRGIGNFRDWPRYFGLSKPTDNPDYDVRKPRPDEVRLQKLFVILQMTYVGAPMIYYGD
                     EVGMWGANDPDCRKPMAWDDIEYAPEATKPDGTPRPPDPVGVDRDLLAHYRRLIAIRR
                     AHPALLVGAYRTLVADDSTGLFAFERTEGDDRVIVVLNREAAPLAATLPAGGTACFED
                     QVTGSRFEASGDIVTVGVPGTWGLVLTACKLR"
     gene            1911..3413
                     /locus_tag="AC16YU_05970"
     CDS             1911..3413
                     /locus_tag="AC16YU_05970"
                     /inference="COORDINATES: protein motif:HMM:NF022623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HIR6064803.1"
                     /translation="MKTESPSRAERWVPAMLSIFVLAFLFPVSAVGQAQSMGPLGDLF
                     LAREGGLAHYSSADPSGGNDDRRPVAPGETLVLVDHEGAGVVRRWWITIAPRENVEIL
                     RHLIVRCWWDGEPSPSVEVPVADFFGMGFGLWKDFISMPLNMTSGGYNAYWPMPFRRS
                     ARITVQNTGTVPVASFYFNVDIRTFDRLPDDALYFHAQYRQVRTESGAPVTVLETTGR
                     GHYVGTLLSMQPERGRHFSYLEGDERVFVDGETEPSVIGTGTEDYFSSGWYYVTGEYS
                     APYHGVTVKDPETGRINTYRWHIEDPIPFRESLRFDIEHGGTNDTPGVEYSSVAFWYQ
                     THPHALFPPIPEHLVPLSGISGPEIEAEGLIDRARVTGGELRVQDMTEFEGSWSGDAH
                     LWWVLGGPGDRLTLPLDVEEAGPYELVGFFTRAGDYGVIRAIVNGEPAGALMDGFSTR
                     VEPTGPVSFGLVQLREGENELVLELIGKDVRSAGFGDGYLVGIDGFTLRR"
     gene            complement(3446..4936)
                     /locus_tag="AC16YU_05975"
     CDS             complement(3446..4936)
                     /locus_tag="AC16YU_05975"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6064804.1"
                     /translation="MNPMTGRSTRLAVALLAAIVLGPVAAAPARQARPDGDPREKPRN
                     VVFILSDDHRYDFMGFHPDGPDFLETPSLDRIAAEGAHLANAFVTTSLCSPSRASILT
                     GQYAHRHGIVDNSSPIPEGTRFFPEDLQRAGYRTGFFGKWHMGEVDDMPQPGFDRWVS
                     FRGQGVYRDPLLNFDGEQLQQTGYVTDLLTDHALDWLGEEDERPFFLYLSHKAVHAEF
                     VPAERHAGRYADVAIPYPASMSNTESNYLGKPHWVKEQRYSWHGVDYAYHGQLDFDDF
                     YRSYSEALLAVDESVGRVLDYLEKAGLAGNTVVFYMGDNGFLLGEHGLIDKRNAYEES
                     IRVPMLAWAPGFIEPGTTVQGLIRNIDVAPTILDLAGAETGIAMDGRSFVPMLTGSSD
                     AAAEGREFLYEYYWEHAFPHTPTTFALRDDRYKFIYYHGVWDTSELYDLSTDPKEQHN
                     LVSAPQHRETVNRMRSRLFERLEAADAMFVPMRRGTWQAGERRLRN"
     gene            complement(4957..>7095)
                     /gene="bglX"
                     /locus_tag="AC16YU_05980"
     CDS             complement(4957..>7095)
                     /gene="bglX"
                     /locus_tag="AC16YU_05980"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HIR6064805.1"
                     /translation="NALVDSLLAEMTLEEKLGQLAQYRGEWSDTGPRVPAGGEDDIRA
                     GRVGSFLGVYGAEYTREMQRLAVEETRLGIPLLFAHDVIHGFRTIFPVPLAEASSWDP
                     ESVEQSARIAALEGAAHGLHWTFAPMVDIARDPRWGRIVEGSGEDPYLGSVMAAARVR
                     GFQGEDLSLPSTLLACAKHFAAYGGAEGGRDYNTVDISERTLREVYLPPFKAAVDAGV
                     ATLMASFNEIGGDPAHGSRFLMTDILRDEWGFDGFVVSDYTGIMELMPHGIAATSEEA
                     GMRALRAGVDMDMVAGIYVGDLPGMVREGRLEEEVVDRSVRLVLEAKYRAGLFEDPYR
                     YSDAQRQASVTLTPEHRRAARDLATRSVVLLRNDGGLLPLDPSARRIAVIGPLADDAR
                     VMLGGWAAAGRAEDAVTIIQGIRDAVSPETEIVYARGSGVEDMDRSGFGAAVAAARRA
                     DVAILVVGEHHDMSSEALNRTSLDLPGVQQELVEAVHATGTPVVAVLVNGRPLSVSWL
                     DENVPSILETWFLGVEAGNAVADVLFGAVNPSGKLPVTFPRNVGQVPIYYAHKNTGRP
                     PDPDNRYTSKYIDVPWTPLYPFGYGLSYTTFAYSGLQLGYPEIGVDGTIEVSVDVTNS
                     GSVEGVEVVQLYLRDDVATVTRPVRQLRRFERVALVPGETKTVRFTLGPEDFEFLDLD
                     LKPVVEPGTFTVFVGGNSRDTIEAQFRITG"
BASE COUNT         1094 a         2461 c         2430 g         1110 t
ORIGIN      
        1 ccccgcccac tccccctccg accccggcga gttcgtgccc cgctgggcca agtccgccgt
       61 ctggtaccag atctttcccg aacgtttccg caacggggac ccgtcgaatg accccaccgt
      121 gcgcgacatc gcgggggcgg atccgcagga gcctccgaag gtctggcacg tgcatccgtg
      181 gggcagcgac tggtaccggc tccagtcctg ggagatggag aacggcgagc ccgagctctg
      241 gaagcacctc ctccgccggc gctacggcgg agacctgcag ggcatcatcg accggctgga
      301 ctacgtggat tccctgggag tgaccgcgct ctacctgaac cccgtcttcg actcgccctc
      361 gctgcacaag tacgacggcg ccagctacca ccacgtcgat ccgaatttcg gacccgatcc
      421 cgacggcgac cgggccatga tcgcggagga ggacccgctc gatccatcga cctgggtctg
      481 gacgtcggcc gaccggctgg cgctcgagct cgtgggcgag gtgcaccgcc gcggcatgcg
      541 gatcatcttc gacggcgtct tcaaccacat gggaaggaac agcttcgcct tccgggacct
      601 cgcggagaag cagcaggact cgccctacgc cgactggttc gtggtccggt cctgggacga
      661 tccggcggcc ggcacgcggt tcgagtactc cgggtgggcc ggcgtgcgct ccctccccga
      721 gttccgcgag gacgagaacg gcatcgtgga aggcccctcc gcctacatct tcgccgccac
      781 cgagcggtgg atgaaccccg acgggcgcgg aaccgcgcac ggcatcgacg gctggcgcct
      841 ggacgtagcc tacgatgtga gccatgcttt ctggaagcgg tggcgtagcc acgtccgctg
      901 gatcaacccg gaggcctatc tgaccgcgga gctcgtggcc ccgcccgagc gggtcgtgcc
      961 gtacctggag ggcgacgagt tcgacggcga gatgaactac aacttcgcct tcaccgccgc
     1021 cgaattcctg ttcaacccgg agccgatgcg gatcacgccg acggccttcg atcgcgagct
     1081 cgaacgcctg cgaacgatgt accccgcggg cgtcgcgtac gtcatgcaga acctcttcgg
     1141 cagccacgac tcgaatcgca tcggctcgca catcgtgaac cgcggaatcg gcaatttccg
     1201 cgactggccc cggtacttcg gcctctccaa gccgacggac aaccccgact acgatgtccg
     1261 caagccgcgg cccgacgagg tccggctgca gaagctgttc gtgatcctgc agatgacgta
     1321 cgtgggcgcc cccatgatct actacgggga cgaggtgggg atgtggggcg ccaacgaccc
     1381 cgactgccgc aagccgatgg cctgggacga catcgagtac gcgcccgagg ccacgaagcc
     1441 ggacggcacg ccgaggccgc cggacccggt cggggtggac cgcgacctgc tagcgcacta
     1501 ccggcggctc atcgcgatac gccgggcgca tcccgcactg ctggtcggcg cgtaccggac
     1561 gctggtggcg gacgactcga ccgggctatt cgccttcgag cgcacggagg gggacgaccg
     1621 cgtgatcgtg gtgctgaacc gcgaggcggc gcccctggcg gccacgctgc ccgcgggcgg
     1681 cacagcgtgc ttcgaggacc aggtgaccgg cagccggttc gaggcgtcgg gcgacatcgt
     1741 gacggtcgga gtgcccggca cctggggcct cgtgctcacg gcctgcaagt tgcgttgatc
     1801 ccgttccgct gacgtttccc ccgctcagcc cttcgcccgt gacgatgagg tccgccgacg
     1861 gtatactgca taccgttccg caggcagatc cctacgggag gtgaacctcc atgaagaccg
     1921 aatcgccgag ccgagccgaa cgctgggttc cggccatgct gtcgatcttc gtcctggcct
     1981 ttctttttcc ggtctccgcg gttggccagg cccagtccat gggtccgctc ggggacctgt
     2041 tcctggcccg ggaaggtggc ctggcgcact attccagcgc cgatccgtcg ggcggcaacg
     2101 atgaccggcg gcccgtggcg cccggcgaaa cgctcgtcct ggtggaccac gagggcgcgg
     2161 gcgtcgtgcg gcgctggtgg atcacgatcg cgccgcgcga gaacgtcgag atcctccggc
     2221 acctgatcgt ccgctgctgg tgggacggcg agccttcacc atcggtcgag gtgccggtcg
     2281 ccgacttttt cggcatgggc ttcggcctgt ggaaggactt catctcgatg cccctgaaca
     2341 tgacgagcgg cgggtacaac gcgtactggc cgatgccctt ccggcggagt gcccgcatca
     2401 cggtccagaa cacgggaacg gtgcccgtgg ccagcttcta tttcaacgtg gatatcagga
     2461 cgttcgaccg cctgccggac gacgcgctgt acttccatgc ccagtatcgg caggtgcgaa
     2521 ccgagtcggg cgcgccggtg accgtgctcg agacgacggg ccgcggccac tacgtgggca
     2581 ccctgctctc catgcagccc gagcgcggac ggcatttctc ctacctggag ggcgacgagc
     2641 gcgtcttcgt ggacggggaa accgagccga gcgtcatcgg aacggggacg gaggattact
     2701 tctcgtcggg ctggtactac gtcaccggcg agtacagcgc gccgtaccac ggggtcaccg
     2761 tcaaggatcc ggaaacgggc cgcataaaca cgtaccgctg gcacatcgag gaccccatcc
     2821 cgttccgcga gagccttcgg ttcgacatcg agcacggggg cacgaacgac acgccggggg
     2881 tcgagtattc gtcggtggcc ttctggtacc agactcatcc ccacgcgctc tttcccccga
     2941 ttcctgagca cctggtgcca ctctcgggga tctccgggcc ggagatcgaa gcggaaggcc
     3001 tgatcgaccg cgcgcgggtc accggcggcg agctccgcgt gcaggacatg acggagttcg
     3061 aggggagctg gagcggcgac gcacacctat ggtgggtcct gggcggaccc ggcgaccgcc
     3121 tgaccctgcc gctcgatgtc gaagaggcgg gtccgtatga gctcgtcggc ttcttcaccc
     3181 gcgccgggga ctacggcgtg atccgggcga tcgtgaacgg cgagccggca ggcgcgctca
     3241 tggacggatt ctccacgcgg gtggagccga cgggtcccgt gtcgttcggc ctggtccaac
     3301 tccgcgaggg cgagaacgag ctcgtgctcg agctcattgg caaggacgtg cgctcggccg
     3361 gcttcggcga cgggtacctg gtcgggatcg acggattcac gctgcgacgg tagcggccgg
     3421 cgatagcggc gcagatcccg cgccctcagt tcctgagccg ccgctcgccc gcctgccagg
     3481 tgccccgccg catcggcacg aacatcgcgt cggccgcctc gagccgttcg aagagccggc
     3541 tgcgcatccg gttcaccgtc tcccggtgct ggggagccga caccaggttg tgctgctcct
     3601 tcgggtccgt ggacaggtcg tagagctcgc tcgtgtccca gacgccgtgg tagtagatga
     3661 acttgtaccg gtcatcccgc agggcgaacg tcgtcggggt gtgcgggaaa gcgtgctccc
     3721 agtagtactc gtacaggaac tcccggccct ctgccgccgc gtccgagctg cccgtgagca
     3781 tcggcacgaa ggagcggccg tccatcgcga tcccggtctc agcgccggcc aggtccagga
     3841 tcgtgggggc gacgtcgatg ttgcggatga gtccctgcac ggtcgtgccg ggctcgatga
     3901 atccgggagc ccaggccagc atcggcaccc ggatcgactc ctcgtaggcg ttccgcttgt
     3961 cgatgagccc gtgctcgccg agcaggaatc cgttgtcgcc catgtagaaa acaaccgtgt
     4021 taccggcgag cccggccttc tccaggtagt ccagcacgcg gcccacgctc tcgtcgaccg
     4081 ccaggagagc ctcggagtag ctgcgataga agtcgtcgaa atcgagctgc ccgtggtacg
     4141 cgtagtcgac gccgtgccac gagtagcgct gctccttcac ccagtggggc ttgccgaggt
     4201 agttcgactc ggtgttcgac atggaggccg ggtacgggat ggcgacgtcc gcataccggc
     4261 cggcatgccg ctccgccggc acgaattcgg cgtgcaccgc cttgtgggag aggtacagga
     4321 agaaggggcg ctcgtcctcc tcgcccagcc agtccagggc atggtcggtc aggaggtcgg
     4381 tgacgtatcc ggtctgctgc agctgctcgc cgtcgaaatt cagcagcgga tcgcggtaga
     4441 ccccctggcc ccggaagctc acccagcggt cgaacccggg ctggggcatg tcgtccacct
     4501 cgcccatgtg ccacttgccg aagaacccgg tccggtaccc cgcgcgctgc aggtcctccg
     4561 ggaagaaccg cgtgccctcg gggatcgggc tcgagttgtc cacgatcccg tgacggtggg
     4621 cgtactgccc ggtgaggatc gatgcgcggg acggcgagca gagcgacgtc gtgacgaagg
     4681 cgttggccag gtgcgcgccc tctgcggcga tgcggtccag gctcggcgtc tccaggaagt
     4741 ccgggccgtc cggatggaag cccatgaagt cgtaccggtg gtcgtcgctc aggatgaaga
     4801 cgacgttccg gggcttctcg cgcgggtccc cgtccggccg cgcctgccgc gccggggcgg
     4861 cggcgacggg gccgaggacg atcgcggcga ggagggcgac ggcgaggcgg gtggagcgtc
     4921 cggtcatcgg gttcatgggc tcgggcgggc tcgggttcac cccgtgatcc ggaactgggc
     4981 ctcgatcgtg tcgcgcgagt tgccgcccac gaagacggtg aacgtcccgg gctcgacgac
     5041 gggcttgaga tcgaggtcca ggaactcgaa atcctcgggg cccagcgtga accgcacggt
     5101 cttggtctcg ccgggaacga gtgccacgcg ctcgaacctc cggagctgcc tgaccggccg
     5161 cgtgacggtc gccacgtcgt cccgcaggta cagctgcacg acctcgaccc cctccacgct
     5221 gccgctgttc gtcacgtcga cggatacctc gatcgtcccg tccaccccga tctccggata
     5281 cccgagctga aggccggagt acgcgaaggt cgtgtagctc agcccgtagc cgaacgggta
     5341 gagcggcgtc cagggcacgt cgatgtactt ggacgtgtac cggttgtcgg ggtccggcgg
     5401 ccttccggtg ttcttgtggg cgtagtagat cggcacctgg cccacgttgc gcgggaaggt
     5461 gaccggaagc ttgccgctcg ggttcacggc gccgaagagc acgtccgcca cggcgttgcc
     5521 cgcctcgacc cccaggaacc aggtctccag gatggacggg acgttctcgt cgagccacga
     5581 gacggacagc ggacgcccgt ttaccagcac cgcgacgacc ggcgtgcccg tcgcgtgcac
     5641 ggcctccacg agctcctgct gcacacccgg gagatcgagc gaggtccggt tcagggcctc
     5701 cgagctcatg tcgtggtgct cgcccacgac caggatcgcc acgtcggcgc gccgggcggc
     5761 cgccacggcc gcgccgaacc cgctccggtc catgtcctcc accccggacc cgcgggcgta
     5821 cacgatctcg gtctccggcg agacggcatc ccggatgccc tggatgatgg tcacggcgtc
     5881 ctcggcccgg cccgcggcgg cccagccccc gagcatgacg cgggcatcgt cggcgagcgg
     5941 accgatcacc gcgatccgcc tcgcggacgg gtccagcggc aggagtcccc cgtcgttcct
     6001 gagcagcacg acggacctcg tcgccaggtc ccgcgccgcc cggcgatgct cgggcgtgag
     6061 ggtgacggac gcctggcgct gggcgtcgct gtagcggtag ggatcctcga aaaggcccgc
     6121 ccggtacttc gcctcgagca cgagccgcac cgagcggtcg accacttcct cctcgagccg
     6181 gccctcgcgg accatcccgg ggagatcgcc gacgtagatg ccggcgacca tgtccatgtc
     6241 cacgccggcc cggagggcgc gcatgccggc ctcctcggac gtcgccgcga tgccgtgcgg
     6301 catgagttcc atgatgcccg tgtagtcgct cacgacgaat ccgtcgaagc cccactcgtc
     6361 gcgcaggatg tcggtcatca ggaagcggct gccgtgcgcc gggtcgccac cgatttcatt
     6421 gaacgaggcc atgagcgtcg ccacgccggc atcgaccgcg gccttgaagg gcggcaggta
     6481 cacctcgcgg agggtccgct ccgagatgtc gaccgtgttg tagtcgcgcc cgccctcggc
     6541 cccgccgtac gccgcgaagt gcttggcgca ggcgagcagc gtcgacggca gcgaaaggtc
     6601 ctcgccctgg aagccccgca cacgcgcggc cgccatcacc gagccgaggt acggatcttc
     6661 cccgctgccc tccacgatgc gtccccatcg ggggtcacgg gctatgtcga ccatcggcgc
     6721 gaacgtccag tgcaggccgt gggcagcgcc ctcgagggcc gcgatgcgcg cggactgctc
     6781 cacgctctcc ggatcccagc tcgaggcctc ggcgagcggc acggggaaga tggtccggaa
     6841 gccgtggatc acgtcgtgcg cgaagagaag cggaatgccg agccgggtct cctcgaccgc
     6901 caggcgctgc atctccctcg tgtactccgc gccgtacacg cccaggaacg agccgacgcg
     6961 gcccgcccgg atgtcgtcct cgccgcccgc gggcacccgc ggacccgtgt cgctccactc
     7021 gccgcggtac tgggcgagct ggcccagctt ctcctcgagg gtcatctccg cgaggagcga
     7081 gtcgacgagc gcgtt
//
LOCUS       DBNHQZ010000223         5721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1421851, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000223 DBNHQZ010000000
VERSION     DBNHQZ010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1421851"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="AC16YU_05985"
     CDS             complement(<1..535)
                     /locus_tag="AC16YU_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064806.1"
                     /translation="MLAFGLALVAMGAWVAPVISLLLPCLLVVGAAVRWLFRRPLMNL
                     AVVIASFAAIADFEEGIQPAEAAYALYYVGFLTHWFWTRILVDREDIFDRPESRVLAL
                     FLVGISLSAILTLLFRGSILLLIGEWMSLSYLALYYPVREAVTRYRNGAAVVLGALVW
                     LGVFVLVRNFIGYEQVLR"
     gene            complement(621..1757)
                     /locus_tag="AC16YU_05990"
     CDS             complement(621..1757)
                     /locus_tag="AC16YU_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIR6064807.1"
                     /translation="MWQLLGTIWRGRRLIAIVTAVAAVASVVITLLIPNTYRATSRLL
                     LPESSGGGMVSAILGDLGSAAQSLLGTSAGDYMRYLAIMNSRRVRMAVVDSFDLVRVY
                     DVAGGRAPLEDAIDILADRTDFVVDIEYDFLSVEVVDESAERAAAMANYLVDLLDQIN
                     NELASGTAARFRRYVEDRYETAQDSRAALLDSIRAFQERFGVFDLQAQTEAFFAQIAD
                     LRVKALQEEIKYEAARNLLGPESPDLEAMSQIVEASNRKYEAALRGREQVLPVAQSQM
                     PDVVRAYLDLEMERAIQERILTFVAPMLEQAKFEEQRTVQALQTVDPASPPARKDGPK
                     RSLICVIATLSSFVLVAFYVLLIDWWNRRHAYFARRLRVATEAP"
     gene            complement(1830..3647)
                     /locus_tag="AC16YU_05995"
     CDS             complement(1830..3647)
                     /locus_tag="AC16YU_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR6064808.1"
                     /translation="MRLTRRRLFPCWAVLCLAATPVAAQVFPTSPPTQLTGAQNPLDA
                     LRSRYGAASSAGSSLALEGALDPDEYVVGPGDLFAILIRGLEMSSAPVAVSADGRLPL
                     PDAGSLAVADLTLSAARDSIVQALLPAFGRTRIEVTLVQPRSFFVHVTGAVPTPGRYQ
                     AAPAARVSTALEMAFADTSRAAVLNRSLRPGFRNVSVVRRDGSELSVDLPRYFSGGST
                     AHNPYLRDGDVVVVTAYDPAYESVRIEGSVPFPGSYDARPDDMLADLLAVAGVRREPG
                     FDAVVRLTRMSDDGSVSTEAFRLADALDGPASATPVRARDHVSVEPASLPGGAASIDG
                     WVFHPGTYPIVEGRTTVRELLERAGGLREDALTSGAYVVRAAPLLQNPDAGMPDSRFA
                     RMPDLSALVAADSMAFMQRMRLTELSIVGRMHFALAYRYQNRVPVDVEGMLEGRVDPL
                     VLRDGDRLVIPRDQHSVYVFGQVLRPGFVAFEPGLTLADYVEMAGGTGPNAGPVMLLR
                     NLDGELIDDPGAPVRSGDLVFVDREKEVTESLAAHQAATQDRQAATQERQVAASERQV
                     ELAAEQSRRDSRYQLASTILSAVSSIGTMIALIISLRRN"
     gene            3786..4730
                     /gene="ispE"
                     /locus_tag="AC16YU_06000"
     CDS             3786..4730
                     /gene="ispE"
                     /locus_tag="AC16YU_06000"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR6064809.1"
                     /translation="MPPPAIRIAAHGPDCGSSVSVLPMLEAPAPAKLNLGLHVLGRRR
                     DGFHDLDTVFLRIPWCDRVTVSESDGLTMTCSAPSLPVDERNLCMMAALLLAAAAGRP
                     PGARLHLEKEVPHGAGLGGGSSDAATTLLLLLRLWGIRPGAVDLARIALDLGSDVPFF
                     LGPPVARGQGRGEVLTPLAMPPALDSATLVVAVPPVAVSTGEAYGMIRPSGLQRPDIA
                     SIVGSGPAAGWRGRLVNDFQEPVCEREPVIGLALKTLAAAGAEYCSLSGSGSAVFGLF
                     GDVDRAERARRALEAMSCRTWVGRPGDPPDSPVLQASD"
     gene            complement(4734..>5721)
                     /locus_tag="AC16YU_06005"
     CDS             complement(4734..>5721)
                     /locus_tag="AC16YU_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6064810.1"
                     /translation="RLEAGFVALDPHSGYVKAWVGGRDLATDWFDHVDKAARQPGSTF
                     KPFVYTAAIDNGWSPYYTLLDDTLRYVDVAGNVWSPGNMGGMSGQMLTLREGLAHSKN
                     SITARLMLEVGPQEVSFIARRMGIKSPLDEVMSLALGTSDVTLLEMATAYSTLANGGL
                     RYEPTAVTRIEDRAGNVLYEAAVAPREALSEQTAYTMVDMLRGVITEGTGLRIRGQYG
                     LTRHDLAGKTGTTQESADGWFMMMHPDLVMGSWVGFNDRRFTFRTDWWGQGAHTALLI
                     VGDFFRTLVDLEDTPIDESARFPSPNVRIDPEAPLRRGQEQEDGQRRGRVGW"
BASE COUNT          981 a         1917 c         1944 g          879 t
ORIGIN      
        1 tgcggagcac ctgctcgtaa ccgatgaaat ttcggaccag cacgaaaacg ccaagccaga
       61 cgagggcacc cagcaccacg gccgcaccgt tcctgtaccg cgtgacggct tcacgcacgg
      121 gatagtagag cgccaggtag gacagcgaca tccactcgcc gatgaggagc aggatgctcc
      181 cccggaaaag cagggtcagg atcgcggaga ggcttattcc aactaggaag agggccagga
      241 cacgggactc cggccggtcg aagatgtcct cgcgatcgac aagaatccgt gtccagaacc
      301 agtgagtcag aaagccgacg tagtagagcg cgtaggctgc ctcggcaggc tgtatgccct
      361 cttcgaaatc ggcgatggcg gcgaaggatg cgatgacgac ggccaggttc atcagcggtc
      421 ggcggaacag ccaccgaacc gctgccccca ccaccagcag gcacggcaac aggagcgaga
      481 tcaccggtgc cacccatgcg cccatggcaa ccagggccag tccgaacgcc agcatggcac
      541 ggaggaggac cactcccgcc gcatgtaccg aagcctctgc cagtggatcg gcgactatgg
      601 cccggaaacc tggcatggcg ctacggagct tccgtcgcga ccctcagcct tcgcgcgaag
      661 tacgcgtgac ggcggttcca ccaatcgatg agcaggacgt agaatgccac gaggacgaag
      721 ctggacaggg tggcgatcac gcagatcagt gaccgctttg ggccgtcctt ccgggcaggt
      781 ggcgaggcag ggtctactgt ctgcagggcc tggacggtgc gctgttcctc gaacttcgcc
      841 tgctcgagca tgggtgccac gaacgtcagt atgcgttcct ggatggcacg ctccatttcc
      901 aggtcgaggt acgccctgac gacatccggc atctgtgact gggcgacagg aagaacctgt
      961 tccctaccgc ggagggccgc ttcgtacttg cgattcgacg cttccacgat ctgggacatg
     1021 gcctcaagat ccgggctctc gggtcccaac aggttgcggg ccgcctcata cttgatttcc
     1081 tcctgcaacg ccttcacccg gagatcagcg atctgtgcga agaaagcctc cgtctgggcc
     1141 tgcagatcga aaacgccgaa gcgttcctgg aatgccctga tcgagtcgag aagggcagcc
     1201 ctcgaatcct gggcggtctc gtaccgatct tctacgtacc ggcggaaacg ggcggccgta
     1261 cccgaggcca gctcgttgtt gatctgatcg agaagatcga cgaggtaatt ggccatcgcc
     1321 gcggcacgct ctgcactctc gtcgaccacc tcgacggaga ggaaatcgta ctcgatgtcg
     1381 accacgaagt ccgtgcggtc ggcgagaatg tcgatcgcat cctccaaggg ggcccgaccg
     1441 cctgccacgt cgtaaacacg caccagatcg aatgagtcga ccacagccat ccggacgcgc
     1501 cggctgttca tgatcgccag gtatcgcatg tagtcgcccg cgctcgttcc caggagggac
     1561 tgcgcagccg aacccagatc gccgaggatt gcgctcacca tgcccccgcc gctggattct
     1621 ggcagcagca gtctggacgt ggcgcggtat gtgttcggaa tcaggagagt aatcaccacg
     1681 ctggcgaccg ccgcgacggc agtcacgatc gcgatcaggc ggcgacctcg ccagatcgtc
     1741 ccgagcagct gccacaagct ctcctcgcct gaagtggttc gctcgtcgtt cgggggacca
     1801 gctgcgtgcg ttccggtcat ctgggggacc tagttccggc ggagactgat gatgagggcg
     1861 atcatggtcc ctatgctgct gacggccgag agaatggtcg acgcgagctg atagcgggaa
     1921 tcgcggcggc tctgctcggc ggcgagctcc acctggcgtt cggacgcggc gacctgcctt
     1981 tcctgggtcg ccgcctgccg atcctgcgtc gccgcctggt gggccgcgag cgactcggtc
     2041 acctccttct cccggtccac gaacaccagg tcgcccgatc gtaccggggc ccccggatcg
     2101 tcgatcaatt cgccgtccag gttgcgaagc agcatgacgg gcccggcgtt ggggccggtg
     2161 cccccggcca tttccacgta gtccgccagc gtaaggccgg gctcgaaggc cacgaatccg
     2221 ggacggagta cctgaccgaa cacgtacacg gaatgttgat cccgcgggat caccaggcgg
     2281 tcgccgtccc gcagcacgag cggatcgacc ctcccttcga gcattccctc cacgtccacc
     2341 ggcacccggt tctggtaccg atatgccagc gcgaagtgca tgcggccgac gatgctcagc
     2401 tccgtgagcc gcatccgctg catgaacgcc atcgaatccg cggccacgag agccgagaga
     2461 tccggcatcc gggcgaaccg cgagtccggc atgccggcat ccgggttctg gagaagcggc
     2521 gcggcgcgca cgacataagc gccggacgtg agcgcgtcct cccggagtcc gccggcccgt
     2581 tcgaggagtt cccggacggt cgttcgtccc tccacgatgg gatacgtccc gggatggaac
     2641 acccagccgt cgatggatgc ggcgcctccg ggaagggacg ccggctcgac cgagacatga
     2701 tcgcgcgcgc ggacaggggt cgccgatgca ggtccatcga gggcgtcggc cagccggaag
     2761 gcctcggtac tcaccgagcc gtcatccgac atgcgcgtga gccggaccac agcgtcgaaa
     2821 cccggttccc ggcggactcc ggcgacggcc agcaggtcgg ccagcatgtc gtcgggccgc
     2881 gcgtcatagg acccggggaa gggaaccgag ccttcgatcc ggacggattc gtacgccggg
     2941 tcgtaggcgg tcacgacgac gacgtcgccg tcacgcaggt aaggattatg cgcagtgctt
     3001 cctccggaga agtaccgggg cagatcgacg ctcagttccg atccgtcacg ccgcaccacc
     3061 gagacgttcc gaaagccggg cctgagggag cggttcaaca cggcggcccg ggacgtgtcc
     3121 gcgaaggcca tttccagcgc ggtggacaca cgggccgccg gggcggcctg gtagcggccg
     3181 ggcgtcggga cggctcccgt gacgtgcacg aagaacgagc ggggctgtac gagggtcacc
     3241 tcgatccgtg tcctcccgaa ggccggcaga agcgcctgca cgatcgaatc ccgggcggcg
     3301 ctcagcgtga ggtccgcaac ggccagggat ccggcatccg gcagcggcaa tcggccatcg
     3361 gcagacacgg ccaccggcgc cgacgacatc tcgaggccgc ggatcaggat cgcgaacagg
     3421 tcgcccggac cgacgacgta ctcgtccgga tcgagcgcac cctcgagcgc gagggacgag
     3481 ccggcggagg acgccgctcc gtaacgactc ctcagggcat cgaggggatt ctgggcgccc
     3541 gtcaactgcg tcggcggcga ggtcgggaag acctgggcgg cgacgggagt cgccgccagg
     3601 cagaggaccg cccaacatgg gaagagccgt cgtcgcgtga gacgcatgga agctcgcaat
     3661 gcggaacgaa ccaacggaaa tcgggcggcg cggccgcgga aagctgcggg ggctgactcg
     3721 taaccgagga cgataggccg cgtacgatcg ggatgcactg cgaaagagcc gaaatctacg
     3781 gtatcttgcc gccgcccgca atccggatcg ccgcccacgg ccccgactgc ggctcctccg
     3841 tctccgtcct gccgatgctg gaagcccccg cgcccgccaa gctgaacctg ggcctgcacg
     3901 tgctggggcg caggcgggac ggcttccatg acctggacac ggtgttcctg cgcataccat
     3961 ggtgtgaccg ggtcaccgtg tcggagtccg acgggctgac gatgacctgc tcggccccgt
     4021 cccttcccgt cgacgagagg aacctgtgca tgatggcggc gctcctcctg gcggcggccg
     4081 ccggccgacc gccgggcgcg aggcttcacc tcgagaagga ggtgccccac ggagccggcc
     4141 tggggggcgg gtcgagcgac gccgcgacca cgctgctcct gctgcttcga ctgtggggca
     4201 ttcggccagg cgccgtggac ctcgcgcgga tcgccctcga tctcggatcg gacgtcccgt
     4261 tcttcctggg gccgcccgtc gcccggggac agggcagggg ggaggtcctc acgccgctgg
     4321 ccatgcctcc ggcgctcgat tcggcgactc tcgtggtggc cgtgccgccc gtcgccgtgt
     4381 cgacgggcga ggcgtacggc atgattcgac cgtcgggact ccagcggccc gacatcgcat
     4441 ccatcgtggg ttccggtccg gcggccggct ggcggggccg cctggtcaac gacttccagg
     4501 agcccgtctg cgagagagag cccgtgatcg gcctcgcatt gaagacgctt gcggccgcgg
     4561 gcgccgagta ctgctcgctc agcggctcgg gatcggcggt tttcggcctg ttcggggacg
     4621 tcgatcgcgc cgagcgggcc cgccgggccc tggaggccat gtcgtgccgc acgtgggtcg
     4681 gtcgcccggg cgacccgccc gattctcccg tactccaggc gtcggactga ccgtcaccag
     4741 ccgacacggc cccggcgttg tccgtcttcc tgctcctgcc cgcgccgcag cggcgcctcg
     4801 gggtcgatgc gcacgttggg cgaggggaag cgcgccgact cgtcgatcgg cgtgtcctcg
     4861 agatcgacga gggtccggaa gaaatcgccg acgatgagca gcgcggtgtg tgcgccctgc
     4921 ccccaccagt cggtgcggaa cgtgaaccgg cggtcgttga agccgaccca cgagcccatc
     4981 accaggtcgg ggtgcatcat catgaaccag ccgtccgcgc tctcctgggt cgtgccggtc
     5041 ttgccggcca ggtcgtgccg ggtcagcccg tactggcccc ggatccgaag gccggtgccc
     5101 tcggtgatca ccccgcggag catgtccacc atcgtgtagg cggtctgctc ggagagggcc
     5161 tcccgcggag cgaccgccgc ttcgtacagg acgttgcccg cccggtcctc gatgcgcgtg
     5221 acggcggtcg gctcgtaccg gagccccccg ttcgcgagcg tggagtacgc ggtcgccatc
     5281 tcgaggaggg tgacgtcgct cgtgccgagg gccagcgaca tgacctcgtc cagcggactc
     5341 ttgatgccca tgcgacgggc gatgaacgag acctcctggg ggccgacttc cagcatgagg
     5401 cgggccgtga tcgagttctt cgagtgggcg agaccctcgc gcagcgtcag catctgcccc
     5461 gacattccgc ccatgttacc cggagaccag acgttgccgg ccacgtcgac gtaccggagc
     5521 gtgtcgtcca gtagcgtgta atacggagac cagccgttgt cgatggcggc cgtgtacacg
     5581 aacggcttga aggtggagcc gggctgcctg gccgccttgt ccacgtggtc gaaccagtcg
     5641 gtcgcgaggt ctcgtcctcc cacccaggct ttaacgtagc cggaatgggg atcgagcgct
     5701 acgaagccgg cctcgaggcg g
//
LOCUS       DBNHQZ010000224         7898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1827155, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000224 DBNHQZ010000000
VERSION     DBNHQZ010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1827155"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1350)
                     /locus_tag="AC16YU_06010"
     CDS             complement(34..1350)
                     /locus_tag="AC16YU_06010"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR6064811.1"
                     /translation="MAPRLAVLAALGLLLAPFAASAQTTYEPTPGNLAAREWYREARF
                     GLFVHWGVYSTLANGEWVMQVQRIPAERYEELPYFFDPYRFDAREWVRLVKEAGMRYI
                     TITAKHHDGFAMYDSRVSDWDIVDRTRSGRDVLAELAEACREEGIRLFFYYSQLDWHH
                     PDYWPRGQTGHTTGRPESGDWNTYIDYMNAQLTELLTGYGPLGGIWFDGMWDRPDADW
                     RLQETYELIHGLQPSALIISNHHQAPIPGEDVQTFEKDLPGHNTAGFNQTAVSASLPL
                     EMSQTISGAWGYNIYDTNFKSTDDLVVGLVRAAGYDANQLLNIGPMPDGRIQPEFQER
                     LRGIGAWLREFGESVYGTRGGPVPPGDWGVSTRTPDAVYLHVLQDDLRALGVEWPRGL
                     EARSARSLRTGEAVAVTVEDGVLVVGLPVRPASVVDEIIVIDVSDD"
     gene            complement(1461..2282)
                     /locus_tag="AC16YU_06015"
     CDS             complement(1461..2282)
                     /locus_tag="AC16YU_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6064812.1"
                     /translation="MDRSVLAFDVETTGRNPEIDQVVEISVCLYDGSPEPHVYTRRMK
                     PDVPITPGALRVHGISMDDLADAPRFAECADEIEALFRSADVVMGYNVSFDIRFLEQE
                     FRRISRPFSLDGRPVVDPYRIWQTMEPRSLEQACSRFAGGNLLNAHSAADDALAVMPV
                     LEGMRSVFGLEERTWDELAAMTSPDRETWIGPTNHIRWEGDAIVLGFGKYEGQPFLDI
                     ARSRPDYLEWMQRQDFPAHVKSLCRGALSGIDDATFTARIVEFYGPAPVIESAGA"
     gene            complement(2369..6367)
                     /locus_tag="AC16YU_06020"
     CDS             complement(2369..6367)
                     /locus_tag="AC16YU_06020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR6064813.1"
                     /translation="MHRMPARRRLIALCLIAFAFRSGPATAQRPAEMRVVSYPAPDDI
                     SGAVLRDLERDSDGFLWLATDAGMVRFDGQTFRIFTVDDGLPENRARSLYRNRRGELY
                     AVLEEGIVRVDPADPRVRFDPVTVGGGSFPDSALSDPGPLFQDLRGRMWGSDRHRIFR
                     IEGKELLSIWMPETSSEDESASGYLLAQFDDTRLLAVSPSGRMALMDIRNDAMSPFST
                     PDGISGVRAAIAAGSGRVLVGTGEAIYEVTEGAGGTLVPRRLADSPPVTAITRLPDGT
                     VIVGTESAGLLRLDATGALSPLPALPAPGILAAVTDDDGHLFVLTRVGLSILWDPYFR
                     TYLDVVSSAIAPMDHFGEDGLLHVDEDRVRLIQMGLEHPVEQVLLDGVPELSAVTGGA
                     GAVWVGTRTGLVLRLVEGREPERFPLPTEQPVTSMEAGSDGSVWIAQRGRIGVVRVMP
                     DGMMDEYGPDRGIRGQVNVLRFLDGELHAGITARSDYVLEYDPSLDRFRSLGPAAPAD
                     HPVEVLDLAVENGSLWLGTDDGLLRLGSMAAERPASVASLAKVSVLALASDPAGGVWI
                     GTSEGLYRLVAGHFATYDILDEAPGIGVARQAIHLDSEGRPWIGTTRTLSYWRDRPLT
                     LLSTPAPKLRTLTTDGVTLVDPHDISFGVVIRALVAAPEYPGHRAVYRHRLVGLSDAW
                     SPASDEPSVHLMGLNEDSYVLEIQARQVGYSWSPSVRIPFTIRAPWYRSSWVIVVLFV
                     FGAFTVFAVLQVFTSIHERHKVENSLVEKADELSRAKAELEHTVRELDLARGAAERAT
                     AAKSAFLASMSHEIRTPMSGVIGMASLLDQTPLSHEQREYVGVIRSSGESLLGLINDI
                     LDFSKIEAGRVDLDVHPFGLRRLVESAIDTIAHAASSKGIEVTYLLRQGVPSRVSGDS
                     TRLRQILINLLSNAVKFTDTGNVTIQIDKLPDSGEDHHLAFSVADTGLGIPPDRVDAV
                     FDRFTQVDAATSRHYGGTGLGLAICRRLVELMEGEISVQSELGKGSTFRFDIWLGPAD
                     GIEDVEDVPLHILDGRRVLLVESSAALRRMVRHEAAAVGLAVTVAEHPDAIRPEDAAL
                     VETCLVGSPADADVPAALDAVRSRLGPDVPIIRLGRLGEAAPPGAPIVARPLRQRPLL
                     AAVASALGERRVHLEEMPADLPAAAPVPAGHLRILVVEDNIINLKVAVRMLERLGYPA
                     DVAASGVEAIDAVRRARYDLVLMDVRMPDMDGIEAARVIRQELPADRVPRLVAMTANA
                     MGSDREWYRAAGMDDFLAKPFSLGSIGAVLARAAVRNEEDGGSETGERAPDRPPTPSD
                     GTAHRLSDIPAE"
     gene            complement(6596..>7898)
                     /locus_tag="AC16YU_06025"
     CDS             complement(6596..>7898)
                     /locus_tag="AC16YU_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064814.1"
                     /translation="KTGHPNALKAVSGAAPTYPANTTPTAIEPPAGTSWNDFAYVVGG
                     YGWAARFVKSDGKVYTAGDDAQQNVADGSRVPYDQGQDVPYNFACFQCHTTGPSASGS
                     WNGVELDALGTFEEAGVTCEACHGPGSDHVAGAFTGVKPPNQGEALTLGVCQQCHNRG
                     GASNTVLARNGFVRHQQQANELMASQHGNGDGVDLTCGTCHNPHVPVHYPDAAGAGLS
                     GLNMTCNTCHPAQQVLLNDAPKAIQCVDCHMPKAARSALGGPVGNGFVGDVSSHLFAI
                     NPAPVGREAMFTGDGAQVALDGEGTGSVTLDFACLGCHTDKSVAWAGDHAPTVHSASG
                     MHTASESTDDLPTSYALGQNYPNPFNPSTTVDFSLPETASVTLKVFDLGGRLLGTVVD
                     GTMPAGFHTVTLDASGLATGVYLYELRTGSFTATRTMVVSK"
BASE COUNT         1258 a         2629 c         2778 g         1233 t
ORIGIN      
        1 cggtccgggg agtcatgcct tgggccctcc ggctcagtcg tccgacacgt cgatcacgat
       61 gatctcgtcc accacggagg ccgggcgcac gggcagcccg acgaccagga cgccatcctc
      121 caccgtcacc gcgacagcct cgcccgtccg gagactgcgg gccgagcgcg cttcgagacc
      181 ccgcggccac tcgacgccga gcgcgcggag gtcgtcctgg agaacgtgca ggtatacggc
      241 atcgggcgtc ctggtcgaca cgccccagtc accgggcggc acggggcctc cccgcgttcc
      301 gtagaccgac tcgccgaact cacgaagcca cgccccgatt ccgcgcagcc gctcctggaa
      361 ttcaggctgg atgcggccgt cgggcatcgg gccgatgttc agcagctggt tcgcgtcgta
      421 cccggcggcg cgaaccaggc ccacgaccag gtcgtccgtc gacttgaaat tcgtgtcgta
      481 gatgttgtag ccccaggccc cgctgatggt ctgggacatc tcgaggggaa gcgacgcgct
      541 gacggcggtc tggttgaacc ctgccgtgtt gtgtccgggc aggtccttct cgaacgtctg
      601 gacgtcctcg ccggggatcg gggcctggtg gtggttcgag atgatgagcg ccgagggctg
      661 cagcccgtga atgagctcgt aggtctcctg caggcgccag tccgcgtcgg gccggtccca
      721 cattccgtcg aaccatatgc cgccgagcgg gccgtacccc gtcaggagtt cagtcagctg
      781 ggcgttcatg tagtcgatgt aggtgttcca gtcgcccgat tcgggccggc ccgtggtgtg
      841 cccggtctgc ccgcgcggcc agtagtcggg gtggtgccaa tcgagctgcg agtagtagaa
      901 gaacagccgg attccctcct cccggcaggc ctctgccagt tcggcgagca cgtcgcggcc
      961 cgaacgcgta cggtcgacga tgtcccagtc cgacacccgg ctgtcgtaca tcgcgaagcc
     1021 gtcgtgatgc ttggccgtga tcgtgatgta gcgcatgccg gcctccttca cgaggcgcac
     1081 ccattcgcgg gcgtcgaagc ggtaggggtc gaagaagtac gggagttcct cgtaccgctc
     1141 cgcgggaatg cgctgcacct gcatcaccca ttcgccgttg gcgagcgtgg agtacacgcc
     1201 ccagtgcacg aacagaccga accgggcctc ccggtaccac tcccgggcgg ccaggttgcc
     1261 cggagtgggc tcgtacgtgg tctgggcgga cgcggcgaag ggagccagaa gaagcccgag
     1321 ggcggcgaga acggcgaggc gaggagccat gacgggcgcc ggaaagggag aagggagggc
     1381 ggcagagcgc cgcgcttcgc aattaggcaa ccgcgcccgt cggagtcaag accggcccgc
     1441 caggggtttc actcccgtcg ctacgcgccc gcgctctcga tcactggggc cgggccgtag
     1501 aactcgacga tccgcgccgt gaacgtcgca tcgtcgatgc ccgagagggc gccccggcac
     1561 aggctcttca cgtgggccgg gaagtcctgc ctctgcatcc actccaggta gtcgggccgg
     1621 ctgcgggcga tgtccaggaa cggctgcccc tcgtacttgc cgaacccgag aacgatcgcg
     1681 tcgccctccc accggatgtg gtttgtcggt ccgatccacg tctcgcggtc gggactggtc
     1741 atggcggcaa gctcgtccca ggtcctctcc tcgagcccga acaccgagcg catgccctcg
     1801 aggacgggca tcacagcgag ggcgtcgtcg gccgcgctgt gcgcattcag gaggttgccg
     1861 cccgcgaagc ggctgcaggc ctgctcgagg ctccgtggct ccatggtctg ccagatcctg
     1921 tacggatcga cgaccggacg accgtcgagg gagaaggggc gcgagatgcg cctgaactcc
     1981 tgctccagga atcggatgtc gaacgagacg ttgtatccca tgacgacgtc ggccgaccgg
     2041 aagagcgcct ctatctcgtc cgcgcattcg gcgaaccggg gtgcgtccgc gaggtcgtcc
     2101 atcgagattc cgtgcacgcg gagcgcaccg ggcgtgatgg gcacgtccgg cttcatgcgg
     2161 cgcgtgtaga cgtgcggctc cggtgagccg tcgtacaggc agaccgagat ctctacgacc
     2221 tggtcgattt cggggttgcg gccggtcgtt tcgacgtcga aggcgagaac ggagcgatcc
     2281 atgagggcgg aagttcggat ccggatgacc cccgaaggat aggagcccgc ctgtgacagc
     2341 cccgtgtcac gcacatatgt gggacggcct actcggcggg aatatccgac aggcgatgcg
     2401 ccgtgccgtc agacggggtc ggcggacgat caggggcgcg ctccccggtt tccgatcccc
     2461 cgtcctcttc gttccggacc gcagcccgcg cgagcaccgc cccgatgctg cccaggctga
     2521 acggcttggc caggaagtcg tccatgcccg ccgcgcggta ccattcccgg tcgctgccca
     2581 tggcgtttgc ggtcatggcg acgagccgtg ggacccggtc cgccggtagc tcctgccgga
     2641 tgacccgggc cgcctcgatt ccgtccatgt cgggcatgcg cacgtccatg agcaccaggt
     2701 cgtaccgggc ccggcgcacg gcgtcgatcg cctcgacgcc gctggccgcc acgtcggccg
     2761 ggtacccgag ccgctcgagc atccggacgg ccaccttcag gttgatgatg ttgtcctcga
     2821 cgacgaggat gcgcaggtgt cccgcgggca ccggggccgc ggccgggagg tcggccggca
     2881 tctcttcgag gtgtacgcgc cgctcgccga gcgccgaggc gacggcggcc agcagtggcc
     2941 gctggcgcag cggacgggcg acgatgggcg ccccgggcgg cgcggcctcg cccaggcgcc
     3001 ccaaccggat gatcggcacg tcgggcccca ggcggctccg gacggcatcg agcgcagccg
     3061 gtacgtccgc gtcggcgggc gagccgacga ggcaggtctc cacgagggcg gcgtcctccg
     3121 gacggatcgc gtccgggtgc tcggccacgg tcaccgcgag tccgaccgcg gccgcctcgt
     3181 gacggaccat gcggcgaagt gcggcggacg attccacgag caggacccgt cggccgtcca
     3241 ggatgtgcag tgggacatcc tccacgtcct cgatgccgtc ggccggtccg agccagatgt
     3301 cgaatcggaa ggtggagccc ttgccgagct cgctctggac cgagatctcg ccctccatga
     3361 gctcgaccag gcgccggcag atcgcgagcc cgagcccggt acctccgtaa tggcgggacg
     3421 tcgcggcatc gacctgcgtg aagcggtcga acaccgcgtc cacccggtcg ggcgggatgc
     3481 ccagcccggt gtcggccacg gagaaggcca ggtggtggtc ctcgccgctg tcggggagct
     3541 tgtcgatctg gatcgtgacg tttcccgtgt cggtgaactt gacggcgttc gagagcaggt
     3601 tgatgaggat ctggcggagg cgcgtggagt cgccggacac ccgggacgga acgccctgcc
     3661 gcagcaggta cgtcacctcg atgcccttgg acgaggccgc gtgggcgatg gtgtcgatgg
     3721 ccgactcgac gaggcggcgc aggccgaacg ggtgcacgtc cagatccacc cggccggcct
     3781 cgatcttgga gaagtccagg atgtcgttga tgaggccgag caggctctcg ccggaagagc
     3841 ggatcacgcc cacgtactcg cgctgctcgt gagacagcgg cgtctggtcg agcaggctgg
     3901 ccatgccgat gacgccgctc atgggcgtgc ggatctcgtg gctcatgctg gcgaggaacg
     3961 cgctcttggc cgccgtggcg cgctcggccg ctccgcgggc gagatcgagc tcgcgcacgg
     4021 tgtgttcgag ctcggccttg gcccgggaga gctcatcggc cttctcgacg agcgagttct
     4081 cgaccttgtg ccgctcgtgg atcgaagtga agacctggag tacggcgaag acggtgaatg
     4141 cgccgaaaac gaacaggacc acgatgaccc agctcgagcg gtaccacggc gcgcggatcg
     4201 tgaagggaat ccgcacactc gggctccagg aatagccgac ctggcgagcc tggatctcga
     4261 ggacgtagga gtcctcgttc agccccatca ggtgcacgga gggttcgtcg ctcgccgggg
     4321 accaggcatc ggagagtccg acgagccgat ggcggtagac tgcgcgatgc cctggatact
     4381 ccggggcggc gaccagcgcg cggatgacga ctccaaagga gatgtcgtgg gggtccacca
     4441 gcgtcacccc gtccgtcgtc agggtacgga gtttgggggc gggtgtggac agcaacgtga
     4501 ggggccgatc ccgccagtag gaaagggtgc gggttgtgcc gatccacgga cggccctccg
     4561 aatcgaggtg aattgcctgc cgcgccacac cgatgcccgg cgcttcatcc aggatgtcat
     4621 aggtcgcgaa atggcctgcc acgagccggt agaggccttc ggacgtaccg atccatacgc
     4681 caccggcggg gtccgaggcc agggcaagca cactcacctt cgcgagggat gccaccgacg
     4741 ccggccgttc ggccgccatg ctcccgaggc gcagcagtcc gtcgtccgtc cccagccaga
     4801 gcgagccgtt ctcgacggcg aggtccagga cctccaccgg gtgatcggcg ggagcggccg
     4861 ggccgagcga ccggaagcgg tcgagcgacg ggtcgtactc gaggacgtaa tccgatcggg
     4921 cagtgatccc cgcgtgcagc tcaccgtcca ggaaacgaag gacattcacc tggccccgga
     4981 ttcccctgtc ggggccgtac tcgtccatca ttccatcggg catgacgcga acgacgccga
     5041 tgcgcccacg ctgcgcgatc cataccgaac cgtccgatcc ggcttccatc gaggtgaccg
     5101 gctgctccgt agggagggga aaacgctcgg gctcgcgccc ctcgacaagt cggagaacca
     5161 gtcccgtacg cgtgcccacc cagaccgcgc cggctccgcc cgtcactgcg gaaagctcgg
     5221 gcaccccgtc gagaagcacc tgctctactg gatgctcaag acccatctgg atcagccgga
     5281 cccggtcctc gtccacgtgc agtagtccgt cctcgccgaa atggtccata ggcgcgatcg
     5341 cgctcgatac cacgtcgaga tacgtccgga aatacggatc ccacaggatc gatagcccga
     5401 cccgcgtaag cacgaacagg tggccgtcgt catccgttac ggccgccagg attcccggag
     5461 cgggaagtgc cggaagcgga ctcaaggccc ccgtggcatc cagtcgcagg agtcccgccg
     5521 attccgttcc gacgatgacg gttccatcgg gaagcctggt aatcgcggtg accgggggag
     5581 agtcggcgag ccgacgcgga acgagggtcc cgcccgcgcc ctcggtgact tcgtagatcg
     5641 cctctcctgt gccgacgagg acgcggccgc tgcccgcggc gatcgctgcg cgcacgccgg
     5701 agatcccatc cggcgtgctg aagggcgaca tggcatcgtt acgaatgtcc atgagtgcca
     5761 tccgtccact tggagagacg gcgagaagcc tcgtgtcatc gaactgtgcg aggagatagc
     5821 ccgaggccga ttcgtcctcc gacgacgttt caggcatcca gatggagagc agctccttgc
     5881 cctcgatccg gaatatccgg tgacggtccg atccccacat tctcccccgg aggtcctgga
     5941 agagcgggcc cggatcggac agcgcggagt ccggaaagga gcctccgccc accgtaacgg
     6001 ggtcgaaacg tacccgtgga tccgccggat cgacccggac gatgccctct tccaggacgg
     6061 cgtacagttc gccacgacgg ttccgataca ggctccgggc tcggttctcc ggcagcccgt
     6121 cgtcgaccgt gaagatccgg aacgtctggc cgtcgaagcg gaccatgccc gcatcggtcg
     6181 cgagccagag gaatccgtcg ctgtcgcgct cgaggtcgcg cagcacggcg cccgaaatgt
     6241 cgtcgggcgc gggatacgac acgacccgca tttcggcggg ccgctgcgcg gtcgcgggcc
     6301 cggaacggaa cgcgaaggcg ataaggcaga gggcgatcag ccgacggcgg gcgggcattc
     6361 ggtgcatgaa cgggaggcgg ggtcgatcac cgtcccgtta tcggccgggg gccgtgattc
     6421 gtaagattcc gggggagcgg gcgaggtctc ccgaccgccg cgaatcgcac gggcggggcc
     6481 gtgtgcgccg ggagggccgg cgaccgtgcc cggtgcgggg tgcgggtgaa acgagaaggg
     6541 gccccggcca ctctggccgg agcccctcga gactcctgtc cggatacggc ctctcctact
     6601 tgctcacgac catcgtgcgg gtggccgtga agctgccggt gcgcagctcg tacaggtaca
     6661 cccctgtcgc caggcccgag gcgtccagcg tgacggtgtg gaagccggcc ggcatggtgc
     6721 cgtcgacgac ggttccgaga agccgcccgc ccagatcgaa caccttgagc gtgacggagg
     6781 ccgtctcggg cagcgagaaa tcgaccgttg tcgacggatt gaagggattc gggtagttct
     6841 gcccgagcgc gtacgaggtc gggaggtcgt cggtcgactc ggacgccgtg tgcatgccgc
     6901 tggcggagtg gacggtcggc gcatggtcgc cggcccaggc caccgacttg tccgtgtggc
     6961 agccgagaca ggcgaagtcc agcgtcacgc tcccggtgcc ctcgccgtcg agtgccacct
     7021 gggcgccgtc gcccgtgaac atggcctcgc gcccgaccgg ggccgggttg atcgcgaaca
     7081 ggtgcgacga gacatcaccc acgaagccgt tgcctaccgg gccgccgaga gccgacctcg
     7141 cggccttcgg catatggcag tcgacgcact ggatcgcctt cggggcgtcg ttgagcagaa
     7201 cctgctgcgc cggatggcag gtgttgcagg tcatgttcag gccgctcaaa ccggcccccg
     7261 cggcgtccgg ataatgcacg ggcacgtgcg gattgtggca ggtcccgcag gtgaggtcca
     7321 cgccgtcgcc gttgccatgc tgggaggcca tgagctcgtt ggcctgctgc tggtgccgga
     7381 cgaatccgtt ccgtgcgagg acggtgttcg atgcgcctcc ccggttgtgg cattgttggc
     7441 agacgccgag ggtgagtgct tctccctggt tgggaggctt gaccccggtg aatgcaccgg
     7501 ccacgtggtc gctgcccggt ccgtgacacg cctcgcaggt gactcccgcc tcttcgaacg
     7561 tcccgagcgc atcgagctcc accccgttcc acgagcctga tgccgagggt ccggtcgtgt
     7621 ggcactggaa gcaggcgaaa ttgtacggca cgtcctggcc ctggtcgtac gggacgcggc
     7681 tgccgtcggc cacgttctgc tgggcgtcgt cgccggcggt gtacaccttg ccgtcggact
     7741 tgacgaagcg cgcggcccaa ccgtacccgc ccacgacgta ggcgaagtcg ttccaggagg
     7801 ttccggcggg cggctcgatc gccgttggcg tcgtgttcgc cggatacgtg ggcgccgccc
     7861 cgctgaccgc cttcagggcg ttgggatgcc cggtcttg
//
LOCUS       DBNHQZ010000225         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_880018, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000225 DBNHQZ010000000
VERSION     DBNHQZ010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_880018"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="AC16YU_06030"
     CDS             complement(<1..138)
                     /locus_tag="AC16YU_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR6064815.1"
                     /translation="MKKGNQPGRKQSESRRDFLKKGALAASAVMAGYTSSTAGAARPR
                     PR"
     gene            283..903
                     /locus_tag="AC16YU_06035"
     CDS             283..903
                     /locus_tag="AC16YU_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR6064816.1"
                     /translation="MMRRLPTYAFLVLAMALTGEASAQAVLTLLPVPESRMFVDGTSN
                     SSDWTVEAQEVTGRFSVVGSEDGFAVSEAEVHVPSSSIKSDRGIIMDRLMRGALKVSQ
                     FKDISFVMSAPATGVAADSGFAITAPGTLTIAGASREIELEVFARPGPDGRWRFTGSH
                     GVAMTDYGMRPPTAMFGALVTGNDVVIRFDVVAAQEAAGSASGQDG"
     gene            1079..2338
                     /locus_tag="AC16YU_06040"
     CDS             1079..2338
                     /locus_tag="AC16YU_06040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6064817.1"
                     /translation="MFHTEPDVVVVGAGPVGLFAALNVTARGHRVRIIDKEWRPTAHS
                     YALALHARSLELLEEFSLLGPVLAASHRIRHVGFYEGSDRFAAVDLRALRRDHSFVAV
                     LRQDVLERLLVDALAARGVKVLWNHELAQMDPSEDGVELAVDKLSREGLGYAVEHGEW
                     VVQRSRTMRPAFVIGTDGHRSRVRSAVGIDWPEAGPAQQFAVFEFRTDTDLGDEMRVM
                     LHDGLQNVVWPMPDGYCRWSFEVPGGDAPAVSRIKDRSDVQLGGVEYPVLAPEALADF
                     LRVRAPWFRGTIEELRWRLLVRFERRRATGLGKGRVWLAGDAAHMTGPVGVQSMNVGL
                     REARDLAHAVCDVIEGRAGSDRLAAYEAERRTEWGFLLGQDGGVKAPKGVEPRIAALV
                     PGVEALLPASGSDLAELLGQLGLSVPA"
     gene            2562..>3403
                     /locus_tag="AC16YU_06045"
     CDS             2562..>3403
                     /locus_tag="AC16YU_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6064818.1"
                     /translation="MRKATVLVALAVFGLTAFYVSRTDALFPGSLHYTRVGKATFYSA
                     ANGGFEQYTNVPMSELPCQGCHAATLANGTPVNPGTYAPSCSDCHVVPGDSPANAVCL
                     GCHSRQGAEISLSANPNPVIAERFRDVHREAGMGCVDCHTSTEVHGDGGTYASMFEAS
                     HLNTTCDDCHTGDTPVGPVPPAAIQEHAMHLDDIECAACHASTVVSCVNCHFESELEH
                     NKRYYGGPPMNGFMLLVNNLQSGKVGPASFQSLTWNDKEPGNETTFFGMGPFGPHTTK
                     AEGR"
BASE COUNT          521 a         1135 c         1213 g          534 t
ORIGIN      
        1 gcggggcctc ggacgggcgg caccggcggt ggaggacgtg tagccggcca tgacggccga
       61 cgcggcgagt gcgcccttct tgaggaaatc gcgacgggac tcggactgct tccgacccgg
      121 ctggttgccc ttcttcatca cggaacggga tttgcgtaga gatgatgggg tcggcgggcc
      181 ggatcctcct gggcgggccg actgcttcga agataattgg caaaccgatc gaaaccacac
      241 cgactcgcgg atgttgtcga aatccgaagt ccaacgagag ccatgatgcg ccgcttgccc
      301 acatacgcct tcctggtcct ggcgatggcc ctcaccggag aggcgtccgc acaggcggtc
      361 ctgaccctct tgcccgtgcc cgagagccgc atgttcgtgg acgggacgtc caactcgagc
      421 gactggacgg tcgaggccca ggaggtgacg ggccgcttct ccgtcgtcgg cagcgaggac
      481 ggcttcgccg tctccgaggc cgaggtgcac gtgccgtcga gcagcatcaa gagcgatcgc
      541 ggcatcatca tggaccgact gatgcgtgga gcgctcaagg tgtcccagtt caaggacatc
      601 tccttcgtca tgtctgcccc ggccaccggc gtagcggccg acagcgggtt tgcgatcacg
      661 gcgcccggga cgctcaccat cgcgggtgcg agtcgcgaga tcgaactcga ggtcttcgcc
      721 cggcccgggc cggacgggcg gtggcggttc acggggagcc acggcgtcgc gatgaccgat
      781 tacggcatgc gccctcccac ggccatgttc ggggccctcg tgaccggcaa cgacgtggtg
      841 atccgattcg acgtggtggc cgcccaggag gcggcgggat cggcgtccgg acaggacggc
      901 tgacgggcgg gggacgggcg ccgggccgcg aacaccggca cccggcgtgg gcgattcccc
      961 gccagatacg tacgtcattc ccgtacaggc ttttcggtac cttgctctcc ccgtgcaacg
     1021 tgccgattgg ggtgcttgca ggcggttcaa gccttctgcc gcacccgcag gtaatcccat
     1081 gttccatacc gagccggatg tagtggtcgt cggcgccggg cccgtcgggc tgttcgccgc
     1141 gttgaacgtc accgcccgtg gacaccgggt ccgcatcatc gacaaggaat ggcggccgac
     1201 cgcgcacagt tatgcgctgg ccctgcacgc gcggtccctg gagcttctcg aggagttctc
     1261 cctgctcggc ccggtgctcg cggcgtcgca ccggatccgt catgtgggct tctacgaggg
     1321 cagcgaccgg ttcgcggccg tcgacctgag ggctctgcgc cgcgaccact cgttcgtggc
     1381 ggtcctgcgc caggacgtcc tcgagcggct gctagtcgat gccctcgcgg cgcgcggggt
     1441 caaggtgctc tggaaccacg agctcgccca gatggacccg tcggaagacg gcgtggagct
     1501 ggccgtggac aagctctcgc gcgaggggct gggatatgcc gtggagcacg gggagtgggt
     1561 cgtgcagcgc agccgcacga tgcgtcctgc gttcgtcatc ggcacggacg ggcatcgctc
     1621 ccgcgtccgg agcgcggtag ggatcgactg gcccgaagcg ggtccggcgc agcagttcgc
     1681 cgtgttcgag ttccggaccg acaccgacct gggcgacgag atgcgggtca tgctccacga
     1741 cgggctccag aacgtggtgt ggcccatgcc cgacggatac tgccggtgga gcttcgaggt
     1801 gccgggcggc gatgcgccgg cggtcagccg catcaaggac cgctcggacg tgcagctggg
     1861 cggagtcgag tacccggtcc tcgctcccga ggcgctggcg gatttcctcc gcgtccgcgc
     1921 gccgtggttc cgcggtacga tcgaagagct gcggtggcgc ctcctcgtgc gcttcgagcg
     1981 gcggcgggcc accggcctcg ggaagggccg cgtatggctc gcgggcgacg cggcgcacat
     2041 gaccggaccc gtcggggtcc agtcgatgaa cgtcgggctc cgggaggccc gtgatctcgc
     2101 gcacgccgtg tgtgacgtga tcgaggggcg ggccggatcg gatcgtctcg cggcctacga
     2161 ggccgagcgc cgcacggagt ggggattcct gctcggacag gacgggggcg tcaaggcgcc
     2221 gaaaggggtg gagccccgga tcgcggcgct cgttccgggc gtcgaggccc tgctgccggc
     2281 ctcgggcagt gacctcgcgg agctgcttgg ccagctgggc ctgtccgtcc cggcataggc
     2341 tttcgcggga ctcgaggggc ggagtagggg aatcccgcag cgacgatgct ccgatcgctg
     2401 tgggtcgggt ggaagccgtc cggcagacgg catcgggcgc ggatgccccc cgttgcgggg
     2461 ttcaccgggt aaggatcgga tgcggcggga agtagcatgg cgtcgtcggc ggggaccccc
     2521 tgccgcgcga tccgacctgc gatccataca cccgatccat catgcgtaaa gctactgtac
     2581 ttgtcgccct ggcggtgttc ggcctgacgg cgttctacgt ctcgcggacg gacgccctgt
     2641 ttccggggag tctccactac acccgggtcg gcaaggcgac cttctactcg gcggccaacg
     2701 gcggcttcga gcagtacacc aacgtgccga tgagcgagct gccctgccag ggctgccacg
     2761 cggccacgct ggcgaacggc acgccggtca acccgggcac ctacgctccg agctgcagcg
     2821 actgccacgt cgtccccggg gattcgcccg ccaacgcggt gtgcctcgga tgccacagcc
     2881 ggcagggagc ggagatctcg ctgtccgcga atccgaaccc cgtgatcgcc gagcgcttcc
     2941 gtgacgtgca ccgcgaggcc ggaatgggct gcgtggactg ccatacctcg accgaggtgc
     3001 acggcgacgg cggcacctac gcatcgatgt tcgaggcgag ccacctcaac acgacctgtg
     3061 acgactgcca cacgggcgac accccggtcg gcccggtgcc acccgccgcc atccaggagc
     3121 acgcgatgca tctcgacgac atcgagtgcg ccgcctgcca cgcatcgacc gtcgtgtcgt
     3181 gcgtgaactg ccacttcgag tcggagctcg agcacaacaa gcgctactac ggaggcccgc
     3241 cgatgaacgg cttcatgctc ctcgtgaaca acctgcagtc cggcaaggtg ggaccggcca
     3301 gcttccagtc gctcacgtgg aacgacaagg agccgggcaa cgagaccacg ttcttcggca
     3361 tgggcccgtt cggcccgcac accacgaagg ccgaaggacg caa
//
LOCUS       DBNHQZ010000226         7562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2505747, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000226 DBNHQZ010000000
VERSION     DBNHQZ010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2505747"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="AC16YU_06050"
     CDS             complement(<1..487)
                     /locus_tag="AC16YU_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6064819.1"
                     /translation="MSRFRFHNDERAAYPLRMAGAVTASLLLAIAAFRLWPLPADEPA
                     RIVFDTRGQEVISMEEILPTRQLERRPRPPAPAPPVVVPDDVELPDGEIELTDVLDYE
                     PPVVGNPTGATTNPQTVFVQPDAGPRPVRIVEPEYTSEARSRRIRAEVIVAVLVDARG
                     RV"
     gene            887..2065
                     /gene="carA"
                     /locus_tag="AC16YU_06055"
     CDS             887..2065
                     /gene="carA"
                     /locus_tag="AC16YU_06055"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR6064820.1"
                     /translation="MIPGIQGPDALRPCKLALADGTVVIGISIGHVGETGGELCFNTS
                     MSGYQEIMTDPSYHGQIMMMTYPHIGNYGAMDADLEAARPMIAGLVVRAFTFRYSNHA
                     ADESLESFMRRNQLVGISGVDTRRLVLHIREAGVMNAVISAVDLDDESLVRKAREWPS
                     MDGLELASRVTVAEAYDYAVSEGPRIAVFDYGVKQNILRSFRDRGCAVRVYPASATLD
                     DVLPWNPDGLFLSNGPGDPRAMPDQVDVARRAAESGLPVFGICLGHQLMALSQGLEVY
                     KMHVGHRGANHPVRFLETGHVEVTTQNHGFAVDPDTLSPDSAELTHMNLNDRTVEGLR
                     YARFAGFSVQYHPEASPGPHDSRYLFDRFLRLVAEVRGVELPAAVAAAVERRTGPATG
                     "
     gene            2144..4972
                     /gene="carB"
                     /locus_tag="AC16YU_06060"
     CDS             2144..4972
                     /gene="carB"
                     /locus_tag="AC16YU_06060"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR6064821.1"
                     /translation="MPKRTDIHKILLIGSGPIIIGQACEFDYSGSQAARALRKEGYEV
                     VLVNSNPATIMTDPVTADKIYLRELTPASIRQIVEIERPDAVLPTMGGQTALNLADRL
                     HQEGYWEKQGIQVIGVDIDAIHITEDRQLFRDLMERVGLDQARSRTAKSLLEAKEIAQ
                     GLGGLPVVIRPSFTLGGSGGGIVWHQDEFDRKVMRGLELSPVHQVLIEECLFGWKEFE
                     LELLRDANDNVIIICSIENVDPMGVHTGDSVTVAPAQTLTDKQFQAMRDASIRAMRSI
                     GTFAGGCNIQFAFDPVSGRMIVIEINPRVSRSSALASKATGYPIAKVAARLAVGYTLD
                     ELPNEVTGTTSACFEPAIDYVVTKIPRFNFEKFEGVDEELTTQMKAVGEVMAIGRTFP
                     ESLQKAWQSLENGYAGLGADRDEVPRPEVRDRLRKPYWDRTLQIRNAFKMGASVDEVA
                     DITKVDPWFLYQIQDMVHLEDRIRERALAQFDADFMREVKRFGFSDQQIAYLVTDDTD
                     EDAVRTFRKGLGVLPSYQLVDTCAGEFPAQTPYFYSTYETQSESERSDREKVVILGSG
                     PNRIGQGIEFDYSCVHGVLAAREAGYEAIMINCNPETVSTDFDVADKLYFEPVFWERV
                     IDVVEHEDPVGVIVQLGGQTALKLARFLDAKGIRILGTSFDHMDLAEDRGRFSALLKE
                     MEIPYPPYGMARSVAEAIENAERIGYPLLVRPNYVLGGQGMRIAINKEEVERYVNQIW
                     KLLPDNPILLDLFLENSIELDVDAVRDDDEVWIAGIMQHIEPAGVHSGDSTAVLPPYS
                     LSEDVLRTVRQYATEIGNRLGVIGLMNVQMVIKDGMVYVIEANPRASRTVPFVAKATG
                     IPVANIATHVMLGEKLASFRAKGMLESKLTGFAIKEPVFSWDKFPEVPKELGPEMKST
                     GESIAFIDELTDEHFSRPYEMRNLYLSR"
     gene            5147..>7562
                     /locus_tag="AC16YU_06065"
     CDS             5147..>7562
                     /locus_tag="AC16YU_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6064822.1"
                     /translation="MARRIIVAAALLLIAAPAAAQDIEIPVDIPYERFVLDNGLTVIV
                     HEDRKAPIVAVNVWYHVGSKNEKPRRTGFAHLFEHLMFNGSENFDQDYFQVLEKVGAT
                     DLNGTTNQDRTNYFQNVPTSALDLALWMESDRMGHLEGAISQEKLDEQRGVVQNEKRQ
                     GENEPYGLVYDLITENTYPEGHPYSWTVIGSMEDLDAASVEDVKDWFSTWYGAANAVL
                     VLAGDLDAATAREKAEHYFGHIPSGPPIAKHDTWIAKRTGEHRMVHQDRVPQARVYKV
                     WNVPEWGSRAAHLLELAGDVLSSGKTSRLYRRLVYDEQIATDVSAGIQAGEIGSQFVI
                     EASARPGVDLAAVEAAIDAEMARFLAEGPAEREMERVRTQALSAFLRGVERIGGFGGK
                     SDVLAEGEVYAGNPEAWKDRYRAIHGATAETVAQAARAWLSDGVFVLEVHPFPEFQES
                     EAVADRSRLPDVGAPPVPSFPDLQRATLSNGLDIVLVERHTTPVVNFRLMVDAGFVSD
                     AGRAPGTASLAMNMLDEGTGTRDALEISEELALLGASLGAGSSLDVSVVSLSALRQNL
                     DASLDLYTDVILNPSFPEADFERLKQLQIASIQREKVSPISMALRVFPRLLYGDDHPY
                     GIPFTGSGTEESVAALTTDDLEAFHRTWFRPGSATLVVTGDITMEEVLPRLERAFGSW
                     PSGEAPGKTIAGVDLREGGVVYLVDRPGSQQSIIFAGHVAPPKDNERELAIQLANTVL
                     GGAFIARVNMNLREDKGWAYGAFTLLWDARGQRPYFAYAPVQSDRTADSMVEIRSEVS
                     AFVGDRP"
BASE COUNT         1242 a         2546 c         2624 g         1150 t
ORIGIN      
        1 tgacccggcc gcgcgcgtcg accaggaccg ccacgatgac ctcggcgcgg atccgccgcg
       61 agcgggcctc ggacgtgtac tcaggctcca cgatccggac cgggcgcggc cctgcgtccg
      121 gctgcacgaa gaccgtctgg ggattcgtcg tggcgcccgt cgggttgccg acgaccggcg
      181 gctcgtaatc cagcacgtcg gtgagctcga tctcgccgtc ggggagctcc acgtcgtcgg
      241 ggaccacgac gggtggcgcc ggggccggcg gccgcggcct gcgctcgagc tgccgcgtgg
      301 gcaggatctc ctccatcgag atcacctcct gtccccgcgt gtcgaacacg atccgcgcgg
      361 gctcatccgc cggcaacggc cacaggcgga acgccgcgat ggcgaggagc aggctcgccg
      421 tcacggcgcc ggccatccgg agcggatagg ccgcgcgttc gtcgttatgg aagcggaagc
      481 gcgacatggt cggtgggccg gccacaaccg caatgtaacg aggcgacgag cggcgggatt
      541 ctggcccttc gccgaatggt gcccggatcg ggccggcccg taggcggggg cgctccgccg
      601 gccgccccgc cggccgcccc gctgtggata actccggcgc cccgcacgga acctgcccgc
      661 cgggcacccg gttgcactcc gcactgtccg aaaggcgcgc cgccgcgcgg cccgcggacg
      721 gcccccgaga accgcaccga ggcacgaatc gaagcgtgac ggctcctcac gaaatccggc
      781 ttccggcccc tcgactggcc ttcccgatcg catcggggag gctttttttt gatgggcggt
      841 cggggccgga tgacggcttc gaccccccac ccagcaccac gaatccatga ttccaggcat
      901 ccagggcccc gatgcactcc ggccgtgcaa gctggcgctg gccgacggca cggtggtgat
      961 cggcatatcg atcggtcacg tcggcgagac cggcggcgag ctctgcttca atacgagcat
     1021 gtccggctac caggagatca tgaccgatcc ctcgtaccac gggcagatca tgatgatgac
     1081 gtacccgcat atcgggaact acggcgcgat ggacgcagat ctcgaggcgg cccgccccat
     1141 gatcgccggg ctcgtggtcc gcgccttcac gttccgctac tcgaaccacg cggccgacga
     1201 gtcgctcgag tccttcatgc ggcgcaacca gctggtcggc atctcgggcg tggacacgcg
     1261 gcgcctcgtg cttcacatcc gggaagcggg cgtgatgaac gcggtcatct cggccgtgga
     1321 tctcgacgac gagagcctgg tgcgcaaggc gcgcgagtgg ccgtccatgg acgggctgga
     1381 gctcgcgtcc cgcgtgacgg ttgccgaggc gtacgactac gccgtctcgg agggcccgag
     1441 gatcgccgtg ttcgactacg gggtgaagca gaacatcctg cgctccttcc gcgatcgcgg
     1501 ctgcgcggtg cgggtgtacc cggcatccgc caccctcgat gacgtcctgc cgtggaatcc
     1561 cgacgggctc ttcctgtcga acggccccgg cgacccgcgg gcgatgccag accaggtcga
     1621 cgtggcgcgc cgggccgcgg agtcgggcct gccggtcttc ggcatctgcc tggggcacca
     1681 gctcatggcg ctctcgcagg ggctggaggt gtacaagatg catgtcgggc accgcggcgc
     1741 gaaccatccg gtccggttcc tggagacggg gcacgtcgag gtgaccacgc agaaccacgg
     1801 attcgcggtc gatccggaca cgctctcgcc cgactccgcc gagctcacgc acatgaacct
     1861 gaacgaccgg acggtcgagg ggcttcgcta cgcgcggttc gccggcttct ccgtccagta
     1921 tcatcccgag gcgtcccccg ggccgcacga cagccgctac ctgttcgacc gcttcctgcg
     1981 cctcgtggcg gaggtccggg gcgtggagct tcccgcagcg gtcgccgcgg cggtcgagcg
     2041 gcggacgggc cctgcgactg gctgatcgat tcgccctcct ccgactctcc acgacccctg
     2101 tccctcggcc ggcaaggccc ggccccccgc gacgaccccg agcatgccca agcgcaccga
     2161 catccacaag atcctgctca tcggctccgg gccgatcatc atcggccagg cctgcgagtt
     2221 cgactactcg ggcagccagg ccgcccgcgc cctgcgcaag gagggctacg aggtggtgct
     2281 cgtgaactcg aacccggcca ccatcatgac cgacccggtc acggccgaca agatctacct
     2341 gcgcgagctg accccggcgt cgatccggca gatcgtggaa atcgagcggc ccgacgccgt
     2401 cctccccacg atgggcggcc agacggccct gaacctcgcg gaccgcctgc accaggaagg
     2461 gtactgggag aagcagggca tccaggtcat cggcgtggac atcgacgcca tccacatcac
     2521 cgaggaccgg cagctgttcc gcgacctgat ggagcgcgtg ggcctggacc aggcccggtc
     2581 ccgcacggcc aagagccttc tcgaggcgaa ggagatcgcg caggggctgg gcgggctgcc
     2641 ggtcgtgatc cgcccgtcgt tcaccctcgg cgggtcgggg ggcggcatcg tgtggcacca
     2701 ggacgagttc gaccgcaagg tgatgcgcgg gctggagctc tcgcccgtgc accaggtgct
     2761 catcgaggag tgcctcttcg gctggaagga gttcgaactg gagctgctgc gggacgcgaa
     2821 cgacaacgtc atcatcatct gctccatcga gaacgtggat ccgatgggcg tgcacacggg
     2881 cgattccgtg accgtggcgc cggcccagac gttgaccgac aagcagttcc aggccatgcg
     2941 cgacgcctcc atccgggcga tgcgctcgat cgggacgttc gccgggggct gcaacatcca
     3001 gttcgccttc gatccggtct cggggcgcat gatcgtcatc gagatcaacc cgcgcgtctc
     3061 gcgctcgtcg gcgctcgctt ccaaggccac gggctacccg atcgccaagg tggccgcgcg
     3121 gctcgccgtc ggctacacgc tcgacgagct gccgaacgag gtgacaggga cgaccagcgc
     3181 ctgcttcgag ccggccatcg actacgtggt caccaagatc ccgcgcttca acttcgagaa
     3241 gttcgagggc gtggacgagg agctcacgac ccagatgaag gccgtgggcg aggtgatggc
     3301 gatcggccgc acgttccccg agagcctgca gaaggcgtgg cagagcctgg agaacgggta
     3361 cgcggggctc ggcgcggacc gtgacgaggt gccccgcccc gaggtccgcg accgcctgcg
     3421 caaaccctat tgggaccgca cgctgcagat ccggaacgcc ttcaagatgg gcgcctccgt
     3481 ggacgaggtt gccgacatca cgaaggtcga tccgtggttc ctgtaccaga tccaggacat
     3541 ggtccacctg gaggaccgga tccgcgagcg ggcgctcgcc cagttcgacg ccgacttcat
     3601 gcgcgaggtg aagcggttcg ggttctcgga ccagcagatc gcctacctcg tcaccgacga
     3661 cacggacgag gacgcggtgc gcacgttccg caagggcctg ggcgtcctgc cctcgtacca
     3721 gctcgtggac acctgcgccg gcgaattccc ggcccagacc ccgtatttct actcgacgta
     3781 cgagacgcag tccgagagcg agcgctccga ccgggagaag gtcgtgatcc tgggctcggg
     3841 gccgaaccgc atcggccagg gcatcgagtt cgactactcg tgcgtccacg gcgtgctggc
     3901 cgcgcgcgag gcgggctacg aggccatcat gatcaactgc aatccggaga cggtgtcgac
     3961 cgacttcgac gtggccgaca agctctactt cgagcccgtg ttctgggagc gggtcatcga
     4021 cgtcgtggag cacgaggatc cggtgggggt catcgtgcag ctcgggggcc agaccgcgct
     4081 gaagctggcc cgcttcctgg acgcgaaggg catccggatc ctgggcacca gcttcgacca
     4141 catggatctc gccgaggatc ggggacgatt ctcggccctc ctgaaggaga tggagatccc
     4201 gtatccgccg tacggcatgg cccggtcggt ggccgaggcc atcgagaacg ccgagcggat
     4261 cggatacccg ctgctcgtgc ggccgaacta cgtgctcggt ggacagggca tgcggatcgc
     4321 gatcaacaag gaggaagtcg agcggtacgt gaaccagatc tggaagctgc ttccggacaa
     4381 cccgatcctg ctcgatctct tcctggagaa ctcgatcgag ctcgacgtgg acgcggtccg
     4441 ggacgacgac gaggtgtgga tcgcgggcat catgcagcac atcgagccgg ccggcgtgca
     4501 ctccggggac tccacggccg tccttccgcc ctattcgctg agcgaggacg tgctgcggac
     4561 cgtccggcag tacgcgaccg agatcgggaa ccgcctcggc gtcatcgggc tcatgaacgt
     4621 acagatggtc atcaaggacg gtatggtgta cgtgatcgag gcgaatcccc gagcctcgcg
     4681 cacggtgccg ttcgtggcga aggcgaccgg cattccggtg gccaacatcg cgacccacgt
     4741 gatgctcggc gagaagctgg cctcgttccg cgcgaagggg atgctcgaat cgaagctcac
     4801 gggcttcgcg atcaaggagc cggtcttctc gtgggacaag ttccccgagg tacccaagga
     4861 gctcgggccc gagatgaagt cgacgggcga atccatcgcc ttcatcgacg agctgaccga
     4921 cgagcacttc tcgcgcccgt acgagatgcg gaacctgtat ctgtcgcggt aggggggcgg
     4981 gtcaggggcg cgtcacatcg atgggtggtc gatcgacgtg tcttctgcgc gtcgctccgc
     5041 gcaacgactc gcgccgggcc gcgtacgaaa agcgttcccg tcgaccggcg tccgcccgcc
     5101 ggctccggga cctggcttct gcatggatca aacagtggat accaccatgg ctcgccggat
     5161 catcgtcgcg gcggcgcttt tgctgatcgc cgcccccgcc gccgcccagg acatcgagat
     5221 ccccgtcgat atcccgtacg aacgcttcgt gctcgacaac gggctcaccg tgatcgtcca
     5281 cgaggaccgc aaggcgccca tcgtggcggt gaacgtctgg taccatgtcg gctccaagaa
     5341 cgagaagccg cgacggaccg gcttcgccca cctgttcgag cacctgatgt tcaacgggtc
     5401 ggagaacttc gaccaggatt atttccaggt gctggagaag gtgggcgcga ccgacctgaa
     5461 cggcacgacc aaccaggacc gcacgaacta cttccagaac gtgccgacgt cggcgctgga
     5521 cctcgcactc tggatggagt ccgaccgcat gggtcacctg gaaggcgcca tctcgcagga
     5581 aaagctcgac gagcagaggg gagtggtcca gaacgagaag cgccaggggg agaacgagcc
     5641 gtacgggctc gtctacgacc tgatcaccga gaacacctac ccggagggcc atccgtactc
     5701 gtggacggtc atcgggtcga tggaggatct ggacgcggct tcggtcgagg acgtgaagga
     5761 ttggttctcg acctggtacg gggcggccaa cgccgtcctc gtgctggccg gggacctgga
     5821 tgccgccacg gcccgcgaga aggccgagca ctacttcggc cacatcccgt ccggcccgcc
     5881 gatcgcgaag cacgatacct ggatcgcgaa gcggaccggc gagcaccgca tggtgcacca
     5941 ggaccgggtc ccgcaggccc gcgtgtacaa ggtctggaac gtgcccgagt ggggctcgcg
     6001 ggcggcgcac ctcctggagc tcgcgggcga cgtcctgtcg agcggaaaga cgtcgaggct
     6061 gtaccggcgc ctggtgtacg acgagcagat cgcgaccgac gtttcggccg ggatccaggc
     6121 gggcgagatc ggcagccagt tcgtgatcga ggcttcggcg aggccgggtg tcgacctggc
     6181 agccgtcgag gccgcgatcg acgcggagat ggcacggttt ctcgccgagg gaccggccga
     6241 acgcgagatg gagcgcgtgc gcacgcaggc cctgtccgcc ttcctgcgcg gggtggagcg
     6301 gatcggaggg ttcggcggaa agagcgacgt cctggccgag ggcgaggtct acgcggggaa
     6361 tccggaggcc tggaaggacc ggtaccgcgc gatccacggg gcgaccgcgg agaccgtggc
     6421 gcaggccgcc cgcgcatggc tctccgacgg ggtgttcgtg ctcgaggtcc atccgttccc
     6481 cgagttccag gagtcggagg ccgtggccga ccgctcccgg ctgccggacg tgggcgcgcc
     6541 gcccgttccg agcttccccg atctgcagcg agcgacgctc tcgaacggcc tggacatcgt
     6601 gctcgtcgag cggcacacga ccccggtcgt gaacttccgg ctgatggtcg atgcgggatt
     6661 cgtgtccgat gccggccggg cgcccggaac ggcgtcgctc gcgatgaaca tgctcgacga
     6721 gggcaccggc acccgggatg ccctggagat ctccgaggag ctcgcgctgc tcggggcgtc
     6781 gctcggcgcg ggatcgagcc tggacgtctc ggtggtctcg ctgtccgcgc tgcggcagaa
     6841 cctggacgcg tcgctcgacc tgtacacgga cgtgatcctg aatccgtcgt ttcccgaggc
     6901 cgatttcgag cggctgaagc agttgcagat tgcgtccatc cagcgggaaa aggtctcgcc
     6961 gatcagcatg gcgctgcgcg tcttcccccg gctgctgtac ggggacgacc atccgtacgg
     7021 gatcccgttc accgggagcg gaaccgagga atcggtcgcc gccctgacga cggatgatct
     7081 cgaggccttc caccggacgt ggttcaggcc gggcagcgcg acgctggtcg tgaccgggga
     7141 catcacgatg gaggaggtgt tgccacggct cgagcgggcg ttcggatcat ggccgtccgg
     7201 cgaggcgccg ggcaagacga tcgcgggcgt cgacctgcgc gagggcggag tggtgtacct
     7261 cgtggaccgc cccggctcgc agcagtcgat catcttcgcg ggccacgtcg cgccgcccaa
     7321 ggacaacgag cgcgagctcg cgatccagct cgcgaacacg gtgctcggcg gggccttcat
     7381 cgcccgggtg aacatgaacc tgcgcgagga caagggctgg gcctacggcg cgttcacgct
     7441 tctgtgggat gcccgcggcc agcggccgta cttcgcgtac gcgcccgtgc agtccgacag
     7501 gaccgccgat tccatggtcg agatccgctc cgaggtgtcc gcgttcgtgg gcgaccgccc
     7561 cg
//
LOCUS       DBNHQZ010000227         5104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2369655, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000227 DBNHQZ010000000
VERSION     DBNHQZ010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2369655"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1762)
                     /locus_tag="AC16YU_06070"
     CDS             complement(74..1762)
                     /locus_tag="AC16YU_06070"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     D-xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIR6064823.1"
                     /translation="MPGEPRYTVGLDFGTNSVRALVVDVRTGAEAGTHVFDFPSGRRG
                     VLLDPADPLLARQSPLDHVAGLEASVRGALDRASGVPGFSADRVIGIGVDTTGSTPIP
                     VDADGRPLGLDPAWADRLDAQAWLWKDHTAHAEARAITEAAARLRPEYLAKCGGTYSS
                     EWFWSKIWHCLAVAPDAFEAAFTWVEHADWVPALLTGTEHPDRIVRGVCAAGHKALYH
                     EAWGGYPDDAFLAALDPRLVRIGATLRRRAATIASRAGGLTSEWADRLGLPAGIPVAV
                     GAFDAHLGGVGSGIGPGTLVKNIGTSTCDMMVAPLGEDLPDVPGLCGIVPESILPGQY
                     GLEAGQSAVGDIFAWFVDVVRPAGKGHAELTAEASSLRPGESGLLALDWHNGNRTVLV
                     DQRLSGLILGLSLHSTPAEIYRALIEATAFGARVIMERFEEYGIAVERVVNCGGISAR
                     NPLVMQIYADVMKRTIYIASSDQTAALGASVAAAVVAGSGAGGYDDFPAATRAMTGVR
                     DVVFDPDPEATAVYDRLYGLYRRLHDAFGAHGHAEPLYDVMKNLLDLRDETRRA"
     gene            complement(1764..3188)
                     /locus_tag="AC16YU_06075"
     CDS             complement(1764..3188)
                     /locus_tag="AC16YU_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR6064824.1"
                     /translation="MKKDIEPVTLGVIIGNRGFFPAHLCADGRTQILAALERAGIRAV
                     IVEADATNNGAIESLDESRLCADLFKRHREVIDGVLVTLPNFGDERAIANTLRFAGLD
                     VPVLIHAFRDASDAMSIAHRRDSFCGKMSACNNLRQYGIRYSLTSEHTMDPDSDEFHA
                     DLLQFAATCRVVRSVRGARLGQIGARPAAFNTVRFSEKLLERTGVTVETLDLSELLGW
                     IRRMTDDDAAVRAKVEDIRGYTSTKGVPDERVVMQAKLGVAVDRFMTDRQLDATAIQC
                     WTSLQEFYGVVPCTCMSMMSNNLMASACEADIAGVLSMWALQQASTKPAALLDWNNNF
                     GKDPDKGVVFHCSNLPKDFFADHAMDYQEIIAGSVGKDNTWGTIVGRVAAGPFTYARV
                     STDDLDGTIRAYVGEGTFTDDKLQTFGGYGVFHVEHLQRLLAHICENGFEHHVAATRA
                     RVSGAVTEAFSKYLGWDTSRHGAA"
     gene            complement(3223..4266)
                     /locus_tag="AC16YU_06080"
     CDS             complement(3223..4266)
                     /locus_tag="AC16YU_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6064825.1"
                     /translation="MKRVTISDVAERAGVSIATVSGVLNGKSGVRESTRERVQATIDA
                     LGYRPVEAARRRLQHPRERTLALIVKEIHNPYFADIVVGAQEAARARGFRVVVMSSEQ
                     DVDLERDAVRSLAARDVDGFIINPLLDDGADLSHLFALTHRNVPFVLLEGLRGILQTT
                     LVDVDNVGAAREATRHVIGLGHERIAHLAGPEYSMHAAERLEGVRRAFSESRRDLPDH
                     LVVHAGASVEDGYRAGLALFDASRTEVPTAVTCYNDLVAIGLMGALAHRGLRVPEDVS
                     VVGYDDIRMAAYLPVPLTTVHVPKREMGRRAAELLIERLTSTSPPRIERVRLEASLVV
                     RSSTAPPRAAKTG"
     gene            complement(4263..>5104)
                     /locus_tag="AC16YU_06085"
     CDS             complement(4263..>5104)
                     /locus_tag="AC16YU_06085"
                     /inference="COORDINATES: protein motif:HMM:NF044297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HIR6064826.1"
                     /translation="YERALYNHILASQDPETGMFAYFMSLRPGHYKTYSTPEESFWCC
                     VGTGMENHVRYGESIWFESADTLWVALFVHSELDWERQGIRITQETRFPDEPVTRFAV
                     AADGPTGFALRVRHPSWATGPLEVELNGEPFDARSAPGSFLSIDRTWQDGDTLTVRFP
                     MELRAEPMPDDPMRIALLVGPIVLAGSLGTQGMPEGGAYAASDTRYVDEPVPDVPVLV
                     TGSADPRDWVRPLAGDPLTFHTVGVGRPHDVVLRPFFRTHHERYTVYWDLRPPGDAAE
                     DDR"
BASE COUNT          730 a         1720 c         1899 g          755 t
ORIGIN      
        1 tgaagtagca cgcgatgatg atccagtcga acggggtcat gggcttgttc ggtctgcggt
       61 ggaggtcggg gattcaggcg cggcgggtct cgtcgcggag gtccaggaga ttcttcatga
      121 cgtcgtacag gggctcggcg tggccatggg cgccgaaggc gtcgtgcagt cgccggtaca
      181 gcccgtacag gcggtcgtag acggcagtcg cctccgggtc cgggtcgaac acgacgtccc
      241 ggacgccggt catcgcgcgg gtggccgccg ggaagtcgtc gtatccgccg gcaccggagc
      301 cggccacgac cgccgcggcg acggaggcgc cgagggccgc ggtctggtcg ctcgacgcga
      361 tgtagatcgt ccgcttcatg acgtcggcgt agatctgcat cacgagcggg ttgcgcgccg
      421 agatgccgcc gcagttcacg acgcgctcga cggcgatgcc gtattcctcg aaccgctcca
      481 tgatgacgcg ggcgccgaac gccgtcgcct cgatgagcgc gcggtagatc tcggcggggg
      541 tcgagtgcag cgacaggccc aggatcaagc ccgagaggcg ctggtccacc agcacggtgc
      601 ggttgccgtt gtgccagtcg agcgcgagga gtccgctctc gccgggcctc aggctcgagg
      661 cctcggcggt gagctccgcg tgtcccttcc ctgcggggcg gacgacgtcc acgaaccacg
      721 cgaagatgtc gcccacggcc gactgtccgg cctccaggcc gtattggccc ggcaggatgg
      781 actcgggcac gatgccgcac aggccgggca cgtcggggag gtcctcgccg agcggggcca
      841 ccatcatgtc gcaggtcgac gtgccgatgt tcttgaccag cgtgccgggc ccgatgccgg
      901 atcccacgcc gcccaggtgc gcgtcgaacg cgcccacggc gaccggaatg ccggcgggca
      961 ggccgagccg gtcggcccat tcgctggtca gcccgccggc ccgcgaagcg atggtggcgg
     1021 cgcgccgccg gagcgtggcg ccgatccgga ccaggcgcgg gtcgagggcg gccaggaagg
     1081 cgtcgtccgg gtacccgccc cacgcttcgt ggtagagggc cttgtggccg gccgcgcaca
     1141 cgccgcgcac gatgcggtcc ggatgctcgg tgccggtcag gagcgccggc acccagtccg
     1201 cgtgctcgac ccacgtgaag gcggcctcga acgcgtcggg ggccacggcc aggcagtgcc
     1261 agatcttgga ccagaaccac tcggagctgt acgtgccgcc gcacttggcc aggtactcgg
     1321 gccggaggcg ggccgcggcc tcggtgatcg cccgcgcctc ggcatgcgcc gtgtggtcct
     1381 tccagagcca ggcctgcgca tcgaggcggt ccgcccatgc cgggtccagc ccgaggggcc
     1441 ggccgtcggc gtcgacgggg atcggcgtcg atcccgtggt gtccacgccg atcccgatca
     1501 cgcggtcggc cgagaagccg ggcacgcccg aggcacgatc gagcgcgccg cggacggacg
     1561 cctcgagccc cgccacgtgg tcgagcgggg actggcgcgc gagcagcgga tcggccggat
     1621 cgagcagcac gccgcggcga ccggagggga aatcgaagac gtgcgtgccc gcctcggccc
     1681 cggtccggac gtccacgacg agggcgcgga cggagttcgt cccgaagtcc agccccaccg
     1741 tgtagcgcgg ttcgcccggc atgtcaggcg gccccgtgcc gggaggtgtc ccagcccagg
     1801 tacttcgaga aggcctcggt caccgcgccc gagacgcggg cccgggtggc ggccacgtgg
     1861 tgctcgaagc cgttctcgca gatgtgagcg aggaggcgct gcaggtgctc gacgtggaag
     1921 acgccgtatc cgccgaacgt ctggagcttg tcgtccgtga acgtgccctc gcccacgtac
     1981 gcgcggatgg tgccgtccag gtcgtccgtg gagacgcggg cgtacgtgaa cgggccggcg
     2041 gccacgcggc ccacgatcgt gccccacgtg ttgtccttgc ccacgcttcc cgcgatgatc
     2101 tcctggtagt ccatcgcgtg gtcggcgaag aagtccttgg gcaggttgga gcagtggaag
     2161 accactccct tgtccgggtc cttgccgaag ttgttgttcc agtcgagcag cgcggcgggt
     2221 ttggtggacg cctgctgcag cgcccacatg gacaggacgc ccgcgatgtc ggcctcgcag
     2281 gcgctcgcca tcaggttgtt cgacatcatg ctcatgcacg tgcacggcac cacgccgtag
     2341 aactcctgca gcgaggtcca gcactggatg gccgtcgcgt cgagctggcg gtccgtcatg
     2401 aaccggtcga cggccacgcc cagcttcgcc tgcatcacga cgcgctcgtc gggaacgccc
     2461 ttcgtgctcg tgtagccgcg gatatcctcg accttggccc ggaccgcggc gtcgtcgtcg
     2521 gtcatgcgcc ggatccagcc gaggagctcg gacaggtcca gcgtctcgac ggtgacgccg
     2581 gtgcgctcga gcagcttctc gctgaagcgg acggtgttga aggcggccgg ccgggcgccg
     2641 atctggccga ggcgtgcccc gcggaccgag cgcaccacgc gacaggtcgc cgcgaactgg
     2701 agcaggtcgg catggaactc gtccgagtcg ggatccatcg tgtgctcgct ggtgagcgag
     2761 taccggatgc cgtactggcg caggttgttg cacgccgaca tcttgccgca gaacgagtca
     2821 cgccggtggg cgatggacat ggcgtcggac gcgtcgcgga aggcatggat gagcacaggc
     2881 acgtccagtc cggcgaaccg gagggtgttc gcgatggccc gctcgtcgcc gaagttgggg
     2941 agcgtgacca gcacgccgtc gatcacctcg cgatgccgct tgaagaggtc cgcgcacagg
     3001 cgggattcgt ccaggctctc gatggcgccg ttgttcgtgg cgtccgcctc gacgatcacg
     3061 gcccggatgc cggcgcgctc gagcgcggcg aggatctgcg ttcgtccgtc cgcgcacagg
     3121 tgggcgggga aaaagccccg gttgccgatg atcacgccga gcgtgacggg ttcgatgtcc
     3181 ttcttcacgg gaatgggtgc gttggttggt ggtcgtgccc cgtcagccgg tctttgcggc
     3241 gcggggcggc gcggtcgacg accgcaccac gagggaggct tcgaggcgca cgcgctcgat
     3301 tctcggcggc gaggtggaag tgagccgctc gatgagcagc tcggcggcgc gccggcccat
     3361 ttcccgcttc ggtacgtgga ccgtggtgag cggaacgggc aggtaggccg ccatgcggat
     3421 gtcgtcgtat ccgacgaccg acacgtcctc gggcacgcgg agcccccggt gcgcgagggc
     3481 gcccatgagc ccgatcgcga ccaggtcgtt gtagcacgtg acggcggtcg ggacctccgt
     3541 ccgggacgcg tcgaaaaggg cgagccccgc ccggtacccg tcctcgacgg acgcccccgc
     3601 gtggaccacg aggtggtccg ggagatcgcg gcgcgactcg ctgaaggccc ggcgtacacc
     3661 ctcgaggcgc tcggcggcgt gcatggagta ctcggggccg gccaggtggg cgatccgctc
     3721 gtggccgagg ccgatcacgt gtcgggtggc ctcgcgggcc gcgccgacgt tgtcgacgtc
     3781 gaccagcgtg gtctgcagga tgccgcggag gccctcgagg agcacgaagg ggacgttgcg
     3841 gtgcgtgagg gcgaagaggt gcgacaggtc ggccccgtcg tcgagcaggg gattgatgat
     3901 gaagccgtcc acgtcgcgcg cggcgagtga gcgcaccgcg tcgcgttcca ggtccacgtc
     3961 ctgctccgag ctcatgacga cgacccggaa gccccgggcg cgggcggcct cctgggcccc
     4021 gacgacgatg tccgcgaagt acggattgtg gatctccttc acgatgagcg cgagggtccg
     4081 ctccctcggg tgctgcagtc ggcgccgggc cgcctcgacc ggccggtatc cgagagcgtc
     4141 gatcgtcgcc tgtacgcgct cccgcgtgct ctcgcggact cccgacttgc cgttcaggac
     4201 gcccgacacg gtcgcgatgg aaacgcccgc gcgctcggcc acgtcgctga tcgtcacgcg
     4261 cttcatcggt cgtcctccgc ggcgtcgccc ggcgggcgga gatcccagta gacggtgtac
     4321 cgttcgtggt gcgtccggaa gaagggcctg agcacgacgt cgtgcggccg tcccaccccc
     4381 acggtgtgga aggtgagggg atcgccggcc agcggcctga cccagtcgcg gggatccgcc
     4441 gagccggtga cgagcaccgg gacgtccggg acgggctcgt ccacatatcg cgtgtcggag
     4501 gccgcgtagg ctccgccctc gggcatgccc tgagtgccca ggctgcccgc gagcacgatc
     4561 gggccgacca gcagcgcgat ccgcatcggg tcgtccggca tgggctcggc gcgcagctcc
     4621 atcggaaagc ggaccgtgag cgtgtcgccg tcctgccagg tgcggtcgat ggagaggaac
     4681 gagccggggg cgctccgcgc gtcgaagggc tcgccgttca gctcgacctc caggggcccg
     4741 gtcgcccagg acgggtgccg cacccggagc gcgaacccgg tgggcccgtc ggcggcgacg
     4801 gcgaagcgcg tcaccggctc gtcggggaac cgcgtctcct gcgtgatccg gatgccctgg
     4861 cgctcccagt cgagctccga gtggacgaag agcgccaccc agagcgtatc ggccgattcg
     4921 aaccagatgc tctcgccgta gcggacgtgg ttctccatgc ccgtgcccac gcagcaccag
     4981 aacgactcct cgggcgtcga gtacgtcttg tagtggcccg gccgcagcga catgaagtac
     5041 gcgaacatcc cggtctcggg gtcctgcgac gccaggatgt ggttgtacag ggcgcgctcg
     5101 taga
//
LOCUS       DBNHQZ010000228         3778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_880387, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000228 DBNHQZ010000000
VERSION     DBNHQZ010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_880387"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="AC16YU_06090"
     CDS             complement(<1..665)
                     /locus_tag="AC16YU_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6064827.1"
                     /translation="METGRLLIDGRWIDAPASDPVFNAFDGAAVGTVARGTADDVDRA
                     LEAARRALGRPWPAHERYRVLMDASARLDAEADELAELIAREGSKTRREARREPHRAA
                     EILRLSAEEGRRATGESLPFDIRPGSENRVGYWTRVPAGIVAAIMPFNDPMAVATHKV
                     GPALAGGNAVVLKPDSRTPFSVLRIARAFVEAGLPDGRLNVITGDGPTVGAALVRDPR
                     VRVV"
     gene            complement(670..2346)
                     /locus_tag="AC16YU_06095"
     CDS             complement(670..2346)
                     /locus_tag="AC16YU_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6064828.1"
                     /translation="MRLFSARRPGLFALLLLAFALPASAQVATEPVDQDAVAAIRKHG
                     LELSALEGTLQVMNDVYGMRLTGSRELDAASEWAAGELRELGLENVGLEAWGPFGRGW
                     TLNGFSVHARGDAFSFPVLAYPKAWSGPSNGRQTGQVVLVNAETPEDVEAIADRIRGN
                     VVLLGAARDVEEPFDPLATRHDEAALLALANWAPDPNPAEGGGRRYGPEAMERFQRQR
                     AVQSAIFAAGPAAVLDGSGRGDYGAIFVSAASVPTPADAPPFGGRVSAYSVDKPEVVP
                     QFTLAIEHFNRLHRLVSGGYEVTVDFELDAEYYDEDPMEYNVVGEMPGTDPEIGDEVV
                     MLGGHYDAWHAGTGMTDNAAGSAVMMEVMRILKETYGELGKAPRRTIRIALWTGEEQG
                     LLGSRGYVAQHFAETAGGGFMGGGGVTEVKAEHEKLSAYYNLDNGTGKIRGVYMQGNA
                     AVEPIFRAWLQPFHDLDAATLTLQNTSGTDHLAFDAVGLPGFQFIQEPIAYGTRTHHS
                     NMDVFDHGLVEDLQQAATVIASFVYHTAERDELLPRKPLVRAEPGATSNR"
     gene            2536..>3778
                     /locus_tag="AC16YU_06100"
     CDS             2536..>3778
                     /locus_tag="AC16YU_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6064829.1"
                     /translation="MSRLPILLLVALVCSPALGQDAPRMTSDVRGTPAGDRRDAVSVR
                     EALREASVVRNVAFRNVGPTVMSGRVADLDVRRDDPTHFFVAYASGGLWKTETGGISF
                     EPLFDRESVMTIGDIAADWERDILWVGTGEKNSSRSSYAGDGLYRSTDGGRTWEHRGL
                     EATQHTGRIVLHPDDPDVLWVAAVGRLYGPSDERGVYKSEDGGATWRKVLFVDDNTGA
                     IDLLAEPGNPEVLYAAMWHRERRAWNFVESGPGSGIYKSTDGGESWTLLTVEGSGFPT
                     GDGVGRIGLDIHGGSPGILFALVDNQYRQPPEEEEEAEGLTRDALREMSAEAFEAVED
                     SVLEAFLRDNDFPDRYDAAGVKAMVRDGRIAPVALVEYLEDANEQLFDTDVVGAEVYR
                     SDDGGATWRKTHQDLIEGLYNT"
BASE COUNT          548 a         1311 c         1381 g          538 t
ORIGIN      
        1 acgacgcgga cgcgcgggtc gcggacgagg gcggcgccga cggtgggccc gtcgcccgtg
       61 atcacgttca gccgtccgtc cgggaggccc gcctcgacga aggcgcgggc gatgcgcagc
      121 acggagaagg gcgtccggga gtcgggcttg agcacgaccg cgttgccgcc ggcgagcgcg
      181 ggtcccacct tgtgggtcgc gaccgccatc gggtcgttga acggcatgat cgccgcgacg
      241 atgcccgccg gaacgcgggt ccagtacccg acccggttct cggagcccgg gcggatatcg
      301 aaggggagcg actccccggt cgcgcgccgg ccctcctcgg ccgaaaggcg caggatctcg
      361 gcggcacggt gcggctcccg ccgggcctcg cgccgcgtct tggatccctc gcgggcgatg
      421 agctcggcga gctcgtcggc ctcggcgtcg aggcgggcgg acgcgtccat caggacccgg
      481 taccgctcgt gcgcgggcca ggggcgtccg agtgcccgcc gggccgcctc gagcgcccgg
      541 tccacgtcgt ccgccgtgcc cctcgccacc gtcccgacgg cagcgccgtc gaaggcgttg
      601 aagacggggt cggacgcggg cgcgtcgatc cagcgcccgt cgatgagcag gcgcccggtc
      661 tccatggccc taccggttgc tcgtcgcgcc gggctcggcc cgcacgagcg gcttgcgggg
      721 caggagctcg tcgcgctcgg ccgtgtgata gacgaaggac gcgatgacgg tcgccgcctg
      781 ctgcaggtcc tcgacgagcc cgtggtcgaa gacgtccatg ttgctgtggt gcgtccgcgt
      841 gccgtacgcg atcggctcct ggatgaactg gaagccgggc agtccgaccg cgtcgaaggc
      901 gaggtggtcc gtgccgctcg tgttctggag tgtgagcgtc gccgcgtcca ggtcatggaa
      961 gggctgcagc caggcccgga agatgggctc gaccgccgcg ttgccctgca tgtacacgcc
     1021 gcggatcttg cccgtgccgt tgtccaggtt gtagtaggcc gagagcttct cgtgctccgc
     1081 cttgacctcc gtcacgccgc cgccgcccat gaagccgccg ccggccgtct cggcgaagtg
     1141 ctgggccacg tagccgcgcg agccgagcag cccctgctcc tcgcccgtcc agagcgcgat
     1201 ccggatggtg cgccgcggcg ccttgccgag ctcgccgtag gtctccttca ggatgcgcat
     1261 gacttccatc atgacggccg agcccgcggc attgtcggtc atgcccgtgc cggcgtgcca
     1321 ggcgtcgtag tggccgccca gcatcacgac ctcgtcaccg atctccgggt ccgtgcccgg
     1381 catctcgccg accacgttgt attccatcgg gtcctcgtcg tagtactcgg cgtcgagctc
     1441 gaagtccacc gttacctcgt acccgccgga cacgaggcgg tgcagccggt tgaagtgctc
     1501 gatcgcgagc gtgaactggg ggacgacctc gggcttgtcc accgagtacg cgctcacccg
     1561 cccgccgaac ggcggggcgt cggccggcgt cggaacggag gcggccgaga cgaagatcgc
     1621 gccgtagtcg ccgcggcccg agccgtcgag gacggccgcg gggccggccg cgaagatggc
     1681 gctctgcacg gcgcgctgcc gctggaaccg ctccatcgcc tcggggccgt accggcgacc
     1741 gccgccctcg gcgggattcg gatcgggcgc ccagttggcg agcgccagca gcgcggcctc
     1801 gtcgtgcctc gtggccaggg gatcgaaggg ctcctcgacg tcacgtgcgg cgccgaggag
     1861 caccacgttg ccccggatcc ggtcggcgat ggcctcgacg tcctcgggcg tctcggcatt
     1921 cacgagcacg acctggccgg tctgcctgcc gttggagggg ccggaccagg ccttcgggta
     1981 cgcgagcacg gggaacgaaa aggcgtcgcc gcgggcgtgc acggagaatc cgttcaaggt
     2041 ccagccgcgt ccgaacggcc cccaggcttc cagcccgacg ttctcgagcc cgagctcgcg
     2101 gagctcgccg gccgcccact ccgaggccgc gtccagctcg cgcgagccgg tgaggcgcat
     2161 cccgtacacg tcgttcatga cctggagcgt gccctcgagg gcgctcagct cgaggccgtg
     2221 cttgcggatg gcggccacgg cgtcctggtc cacgggctcc gtggccacct gggcggaggc
     2281 gggaagcgcg aaagcgagaa gcagcagggc gaaaagcccc gggcggcggg cggagaagag
     2341 tcgcatggcg ggaaaggtct ctggttcacg gcacttcgaa accggagtat accaatcccg
     2401 ggcggcgctc tccgcctctg aatgtgtcaa ttctgtggga ttcggcccgg aaacgcccgt
     2461 gtggggcgat ccccggcaac ggcggtgcga agcgcgcgtg gaccgtagat tccgcgcgcc
     2521 ccatcgaggc cagccatgtc cagactcccg atcctccttc tcgtcgccct cgtctgctcg
     2581 cccgccctcg gccaggatgc gccgaggatg acttccgacg tccggggcac gcccgccggt
     2641 gaccggcgcg atgccgtttc cgtgcgcgaa gccctgcgcg aggcgtccgt cgtcaggaac
     2701 gtcgcgttcc gcaacgtggg tcccacggtg atgagcgggc gcgtggcgga cctggacgtc
     2761 cgacgcgacg acccgaccca tttcttcgtg gcctacgcgt cgggcgggct ctggaagacg
     2821 gagaccggcg gcatctcgtt cgagcccctc ttcgaccggg agtcggtcat gacgatcggc
     2881 gacatcgcgg ccgactggga gcgggacatc ctgtgggtgg ggaccggcga gaagaactcg
     2941 agccggagct catacgccgg cgacggcctg taccggagca cggacggcgg ccggacgtgg
     3001 gagcaccggg ggctcgaggc tacgcagcac acgggccgca tcgtcctgca cccggacgat
     3061 ccggacgtcc tctgggtggc ggccgtcggg aggctgtacg gcccctccga cgagcgcggc
     3121 gtctacaaga gcgaggacgg cggcgccacg tggcggaaag tcctcttcgt ggacgacaac
     3181 accggcgcca tcgacctcct cgccgagccc ggcaaccccg aagtcctgta cgccgcgatg
     3241 tggcaccgcg agcgcagggc atggaatttt gtcgaatcgg ggcccggatc gggcatatac
     3301 aagtccaccg acggcggaga atcctggacg ctcctgaccg tcgagggcag cggattcccc
     3361 accggggacg gggtgggccg gatcgggctc gacatccacg ggggaagccc ggggatcctc
     3421 ttcgcgctcg tcgacaacca gtaccggcag cctccggagg aagaggagga ggcggaggga
     3481 ctgacccggg acgccctgcg ggagatgtcg gccgaagcgt tcgaggcggt cgaagactcg
     3541 gtcctggagg ccttcctgcg cgacaacgac ttcccggacc gctacgatgc cgccggggtg
     3601 aaggcgatgg tccgggacgg acgcatcgcc ccggtcgccc tcgtcgagta cctggaggac
     3661 gcgaacgagc agctcttcga cacggacgtg gtcggggcgg aggtgtaccg gtccgacgac
     3721 ggcggcgcga cctggaggaa gacccaccag gacctcatcg agggcctgta caacacct
//
LOCUS       DBNHQZ010000229         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2369682, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000229 DBNHQZ010000000
VERSION     DBNHQZ010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2369682"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="AC16YU_06105"
     CDS             complement(<1..789)
                     /locus_tag="AC16YU_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064830.1"
                     /translation="MDAHTRRRTAILMVGLACLVPTRLAISQRVPDPNHGDPKLTQWG
                     VLDGNRVRTLFANHGEIARWPDQPSGEWPKGSGHSYVDGVALIVSVQTRDALGGIIKP
                     QSTNYREFIDRDPTSKEPWGWAPLPGYSNRRQESPARSDEPTTWPSEWPDRPVSWAGQ
                     WNGFFGRGVENADVETYFVFDDASDREWLEEPHRFYPCPGDTTRGGLGLEVSARGFQW
                     NHPLAQDVVFWLYEITNECDVDYDDVYFSQYIDWGVGGTDDSGDD"
     gene            complement(794..>2705)
                     /locus_tag="AC16YU_06110"
     CDS             complement(794..>2705)
                     /locus_tag="AC16YU_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6064831.1"
                     /translation="QHVGRLSPSDAYEVQGAFGGPVPGTGGRASFFASGRRLSNEGFL
                     YGRRIVQPVAARPGEGQIVHVDGREVFVPALGDSSYVPMNWGEQTTGQFKVTTRLFGQ
                     NRLTANLLVQNDEGQNYDHLFRYNPEGIPTTFGTSRSLTGTYTHVLGESGFLDLKGAV
                     FTNRVESYVYEDPLDSRYPLDNALRQLGGNFSFVRGGASMGHFRRETETIVGRVDLTS
                     QVSRQHLVKAGVEYKSHELFLDDFEVKNNASTDFMPAVPAAGTPDHVLYTERPREASA
                     FVQDKMEFDFMVVNAGVRMDYFDPRSDVLEDYGRPRTSPRLPTTAKWQISPRFGLAYP
                     LSDLGIFHVAYGHFFQMPPFEFLFTNPDYIYDPEEGLNRAFGYPDLEPQRTVAYEIGL
                     QQAFSGVVLEATAYFKDIRNLLGSRIEVIKPGFDESFQLEKYGRFINRDYGQVRGLIV
                     TLDKRLANGFGLTVDYTFQIAQGNASDPRSVLLDEQAGIESEKQLVPLDWDRRHQLNT
                     TLTLGDPDRWIATLTGRLGSGLPYTPSLADERIGLENSARRRPTATLDLFAKKDVSVL
                     GLAVSVYTRVFNLLDTHNEIQIYSDTGRAFPNLRFLPGEPQGLNSKDEFLDRPDFFSA
                     PRQVTVGFTTRF"
BASE COUNT          480 a          860 c          866 g          499 t
ORIGIN      
        1 gtcgtcgcct gaatcgtccg ttccgcccac gccccagtcg atgtactgtg agaagtacac
       61 gtcgtcgtag tccacgtcgc attcgttggt gatctcgtac agccagaaga cgacgtcctg
      121 cgccaacggg tgattccact ggaagccccg tgccgagacc tccagcccga ggccgccgcg
      181 cgtggtgtcc cccggacacg ggtagaagcg atggggctcc tccagccact cccggtcgga
      241 ggcatcgtcg aagacgaagt aggtctctac gtccgcattc tcgacgcccc gcccgaagaa
      301 gccgttccac tgtccggccc agcttacggg acggtccggc cactccgacg gccacgtggt
      361 cggttcgtcg cttcgcgccg gtgactcctg ccgccgattc gagtatccgg gcagaggcgc
      421 ccagccccac ggctccttcg atgtcggatc ccgatcgatg aactcgcggt agttcgtgct
      481 ctgcggcttg atgatcccgc ccagcgcgtc acgggtctgc accgaaacga tcagcgccac
      541 gccgtcgacg taggagtgcc cgctgccctt cggccattcc cccgagggct ggtccggcca
      601 gcgggcaatc tctccgtggt tggcgaagag cgtacgcacg cggttgccgt cgagcacgcc
      661 ccactgcgtc aatttcgggt cgccgtggtt cggatcgggg acgcgctggg agatggcgag
      721 acgggtcggg accagacagg ccagcccgac catcagaatc gcggttcgtc tcctcgtgtg
      781 agcgtccatg ggttcagaat cgggtcgtga aaccgacagt cacctgccgc ggagcagaaa
      841 agaagtccgg gcggtccagg aactcgtcct tcgagttcag gccctgcggc tcccctggaa
      901 ggaaccggag attcggaaaa gcgcgccctg tatcggagta gatctggatc tcgttgtgtg
      961 tgtccagcag gttgaacacg cgcgtgtata ccgacacggc gagacccagg acggatacat
     1021 ccttcttcgc aaagagatcg agcgttgccg tggggcggcg gcgggcggaa ttctccagtc
     1081 cgatgcgctc gtcggccagg gacggcgtgt agggaagccc cgatcccagg cgcccggtca
     1141 gggtcgctat ccacctgtcc ggatcgccca gcgtcagggt cgtgttgagc tgatgtcgcc
     1201 ggtcccaatc gagcggtacg agctgcttct cggactctat gccggcctgc tcatcgagca
     1261 ggacggatcg gggatcggac gcgttcccct gggcgatctg gaaggtgtaa tcgacggtga
     1321 gaccgaagcc gttggcgagg cgcttgtcga gcgtcacgat gagtcccctc acctgtccgt
     1381 agtcccggtt gatgaagcgc ccgtatttct ccagctggaa cgactcgtcg aatcccggct
     1441 tgatcacctc gatacgagac ccgagcaggt tcctgatgtc cttgaagtaa gccgtcgcct
     1501 cgaggacgac gcccgaaaac gcctgctgaa gacctatctc gtaggccacg gttcgctggg
     1561 gctcgaggtc cgggtagcca aaggcgcggt tgagcccctc ttccgggtcg tagatgtagt
     1621 cggggttcgt gaacaggaac tcgaacggag gcatctggaa gaaatggccg tacgccacgt
     1681 ggaatatccc gaggtccgac agcgggtagg cgaggccgaa tctcggcgaa atctgccact
     1741 tggccgtcgt gggaagcctc ggtgaggttc gagggcggcc ataatcctcg agcacgtcgc
     1801 tccgggggtc gaaatagtcc atccggacgc ctgcattcac caccatgaag tcgaactcca
     1861 tcttgtcctg gacgaaggca ctggcttccc tggggcgctc ggtatacagg acgtgatccg
     1921 gcgtgcccgc ggcgggaacg gccggcatga agtccgtgga cgcgttgttc ttgacctcga
     1981 aatcgtccag aaacagctcg tgactcttgt actccactcc cgccttgacg aggtgctggc
     2041 gcgaaacctg gctcgtgagg tccacccgac cgacgatcgt ctcggtctcc cgccggaagt
     2101 gacccatgct ggccccgcca cgcacgaagc tgaagtttcc accgagctgg cgcagtgcgt
     2161 tgtcgagcgg atagcgggaa tcgagcggat cctcgtagac gtacgactcg acccggttcg
     2221 tgaagacggc gcccttcagg tccaggaaac cgctctcgcc caggacatgc gtgtaggtgc
     2281 ccgtgagcga ccgcgaagtc ccgaacgtcg tcggaatgcc ctccgggtta taccggaaga
     2341 ggtgatcgta attctgcccc tcgtcgttct ggacgagcag gttcgcggtc agccggttct
     2401 gcccgaagag ccgcgtggtg accttgaact ggccggttgt ctgctccccc cagttcatcg
     2461 ggacatatga actgtccccc agggcaggga cgaacacctc gcgtccgtcc acatgcacga
     2521 tctgcccctc accgggtctg gcggcgacag gctgcacgat ccggcggccg tacaggaagc
     2581 cttcgttact cagtcttcgg ccagatgcga agaacgatgc gcggccccct gttcccggga
     2641 ccggtccgcc aaatgctccc tggacctcgt aggcgtcgga aggggagagg cgtccgacat
     2701 gctga
//
LOCUS       DBNHQZ010000230         3591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1150542, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000230 DBNHQZ010000000
VERSION     DBNHQZ010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1150542"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC16YU_06115"
     CDS             <1..356
                     /locus_tag="AC16YU_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HIR6064832.1"
                     /translation="DLVHSGPTYSGHTVDGGRIIVGFDHVGGGLRASTRPITAPRAEG
                     RATADRLTGFAIAGADRRFVWAEARILGDQVIVSSPRVPNPVSVRYAWADNPDRANLY
                     NVEGLPASPFRTDDW"
     gene            complement(416..2743)
                     /locus_tag="AC16YU_06120"
     CDS             complement(416..2743)
                     /locus_tag="AC16YU_06120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6064833.1"
                     /translation="MRRLVAALLLVASTLPAQARQQDLTEADYARAERFLGQNVNALV
                     YGNAVRPVWLEGDAFWYRNTVRGGAEFVLVEPAAGSRARAFDHARLAAAISQVTGEAI
                     GELELPFTTFEFTGDRGSLRFSVGEDLFTCGLADYACTVEEGAGTAAEDERAAMFRRF
                     TATEIPSPDSTKEAFVREHDLWVRDRATGRETRLTTDGEEHYGYATNNAGWTKSDRPV
                     LVWSPDSKKIATFRHDGRAVGMMYLVSTAVGHPELEAWKYPLPGDSLVFRIERVVVHV
                     DEPRVVRLAMPPDEHRSTITDHVVDGGEWVDIEWSPDSRRLVFVSTSRDHRTEILRVA
                     DPETGTVRDVLTEVEDTFYESGFDVVNNRLLDATDEYIWYSQRDDWGHLYLYDLATGR
                     LKNPITSGAWKVLAIEHIDEPSRTIWFRGAGREPGDPYFQYLYSVKMDGSDLRLLTPD
                     SLNHQVAFSPSFRHFVDTASSPDTPQTITLRDAATGAEVVALESADIADLVAAGWKPP
                     IPFTVKARDGETDLYGLMYTPTGLDAGGSYPVLNYIYPGPQTGSVGSRSFVPARSDKQ
                     AVSELGFVVVELDALGSPMRSHSFHAFYYGNMGDNGLPDNITGIRQLAARHPFMDTSR
                     VGIWGHSGGGFASTAGILRHPDFYDVAVSGAGNHDNATYEDDWGEKWQGLLVRNEDGT
                     TSYDNQANHLLAGNLRGKLLLAHGSMDSNVPPNNTLLVVEALMNAGKDFDMLLFPNSR
                     HGFRQGNYWTRRRWDYFVRHLLGVEPPREYAIGGG"
     gene            complement(2820..>3591)
                     /locus_tag="AC16YU_06125"
     CDS             complement(2820..>3591)
                     /locus_tag="AC16YU_06125"
                     /inference="COORDINATES: protein motif:HMM:NF018206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HIR6064834.1"
                     /translation="FSGLVGAVHDARVSFWAPGEEASPGIEAMAELGSKTLLLAEVAD
                     ALADGTALSELSGGGIALSPGSVTLEFEAHEDFPLLTIVSMVAPSPDWFVGATSLPLR
                     EAGGWLPMLEVELPVYDAGTDSGTDYPSENADTQPREPITSLTEPPFDQEQVLGVFRI
                     ERIDATGVSGRDELPVDLDIAAAFPNPAATSVEFRVDGKVPARLAVYDLLGRLLHRGR
                     AAPGDRVRLDVSSWPAGLYLVRAESAAGVRTRTFIVER"
BASE COUNT          517 a         1251 c         1280 g          543 t
ORIGIN      
        1 aggacctcgt gcactctggt ccgacgtaca gcgggcacac ggtggacggc gggcggatca
       61 tcgtcggatt cgaccacgtg ggcggaggcc tccgcgcctc gacgcggccc atcacggctc
      121 cgcgggccga gggccgcgcg acggccgacc ggctcacggg gttcgccatc gcgggcgccg
      181 accgccggtt cgtgtgggcc gaggcgcgca tcctcggcga ccaggtgatc gtctccagcc
      241 cgcgcgtgcc gaaccccgtc tccgtgcgct acgcctgggc cgacaatccc gaccgcgcga
      301 acctgtacaa cgtggagggc cttccggcgt ccccgttccg tacggacgac tggtagctgg
      361 gcgcggccgg gcgggtgcat caggcgccgc gccgctgcgt tcggtcagcc taccctcatc
      421 ccccgccgat cgcgtactcc ctgggcggct cgacgccgag caggtgccgc acgaagtagt
      481 cccagcggcg gcgcgtccag taattgccct gccggaagcc gtgccgcgag ttcgggaaga
      541 gcagcatgtc gaagtccttg ccggcgttca tgagggcctc gacgaccagg agggtgttgt
      601 tgggcggcac gttcgagtcc atcgatccgt gcgcgagcag cagcttgccg cggaggtttc
      661 ccgccagcag gtggttcgcc tggttgtcgt agctggtcgt gccgtcctcg ttgcggacga
      721 gcaggccctg ccacttctcg ccccagtcgt cctcgtaggt ggcgttgtcg tggttgccgg
      781 cgcccgacac cgcgacgtcg tagaagtcgg ggtgccggag gatgccggcc gtcgaggcga
      841 agcccccgcc cgagtggccc cagatcccca cgcgcgacgt gtccatgaag gggtgccgcg
      901 cggcgagctg ccggatgccc gtgatgttgt ccggcaggcc gttgtcgccc atgttgccgt
      961 agtagaaggc atggaacgag tgcgaccgca tcggcgagcc cagggcatcg agctccacga
     1021 ccacgaagcc gagctccgaa acggcctgct tgtcgctgcg ggccggcacg aagctgcgtg
     1081 acccgacgct cccggtctgc gggccgggat agatgtagtt cagcacgggg tagcttccgc
     1141 ccgcgtccag ccccgtgggc gtgtacatga gtccgtacag gtccgtctcc ccgtcgcggg
     1201 ccttgaccgt gaacgggatg ggcggtttcc agccggccgc gaccaggtcc gcgatgtcgg
     1261 ccgactcgag cgccacgacc tcggctcccg tcgcggcgtc acgcagcgtg atcgtctggg
     1321 gggtgtcggg cgacgaggcg gtgtcgacga aatggcggaa ggacggcgag aacgcgacct
     1381 ggtggttcag gctgtccggc gtgagcagcc ggaggtcgga cccgtccatc ttcaccgagt
     1441 acaggtactg gaagtacggg tctcccggct cccggcccgc gccgcggaac cagatggtcc
     1501 ggctcggctc gtcgatgtgc tcgatcgcga gcaccttcca agcgccgctc gtgatggggt
     1561 tcttgagccg tccggtcgcc aggtcgtaca ggtacaggtg cccccagtcg tcgcgctggg
     1621 aataccagat gtactcgtcc gtggcgtcga gcaggcggtt gttgacgacg tcgaagcccg
     1681 actcgtagaa cgtgtcctcg acctcggtaa ggacgtcgcg cacggtgccc gtttcgggat
     1741 cggccacgcg caggatctcg gtcctgtggt cgcgcgacgt ggagacgaac accaggcggc
     1801 ggctgtcggg gctccactcg atgtccaccc actccccgcc gtcgaccacg tggtccgtga
     1861 tggtcgaccg gtgctcgtcg ggcggcattg cgaggcgcac gacgcgcggt tcgtccacgt
     1921 gcacgacgac gcgttcgatc cggaaaacga gcgagtcgcc gggcagaggg tacttccacg
     1981 cctcgagctc cgggtgtccg accgcggtcg aaaccaggta catcatgccg accgcgcgcc
     2041 cgtcatgcct gaaagtcgcg atcttcttcg agtccgggga ccacacgagc acgggccgat
     2101 cgctcttcgt ccagcccgcg ttgttcgtcg cgtagccgta gtgctcctct ccgtccgtcg
     2161 tgagccgggt ctcccggccc gtcgcccggt cccgtaccca gaggtcgtgc tcgcgcacga
     2221 acgcctcctt cgtgctgtcg ggcgacggga tctccgtggc ggtgaaccgg cggaacatgg
     2281 cggcgcgctc gtcctcggcg gccgtgcccg cgccctcttc caccgtgcac gcgtaatcgg
     2341 ccaggccgca cgtgaacagg tcctcgccga cgctgaagcg cagcgagccc cggtcgcccg
     2401 tgaactcgaa cgtggtgaag ggaagctcca gctccccgat cgcctcgccc gtgacctgcg
     2461 agatcgcggc cgcaagacgg gcgtggtcga acgcgcgcgc ccgggagccc gcggcgggct
     2521 cgaccaggac gaactccgcg ccgccccgca ccgtgttgcg ataccagaag gcgtcgccct
     2581 ccagccagac gggccgaacg gcgttgccgt agaccagggc gttcacgttc tggccgagga
     2641 agcgctcggc gcgggcgtaa tcggcctcgg tcaggtcctg ctgccgggcc tgggccggca
     2701 gggtcgaggc gacgagaagt aaggcggcga cgagacggcg catggcggct tcggatggct
     2761 gcggttcggg aaccgcggaa gttgacccgc gcgccccata atggcaaccg ccggagccgt
     2821 caccgttcga cgatgaaggt ccgggtgcgc acgccggcgg cggattccgc cctgaccagg
     2881 tacaggccgg ccggccacga ggacacgtcc agccttacgc ggtcgccggg ggccgcccgg
     2941 ccgcggtgga gcagccgtcc gagcaggtcg tagacggcga gccgtgccgg gaccttgccg
     3001 tccacccgga actcgaccga cgtcgcggcc ggattcggga aggcggccgc gatatcgagg
     3061 tccaccggga gctcgtcccg gccggagact cccgtggcat cgattcgctc gatccggaac
     3121 acgccgagca cctgctcctg gtcgaacggc ggctccgtga gagaggtgat cggctcgcgc
     3181 ggctgcgtgt cggcgttctc cgacgggtag tcggtgccgc tgtccgtgcc ggcgtcgtac
     3241 accgggagct ccacctcgag catcgggagc catccccccg cctcgcgcag cggcagggac
     3301 gtggcgccca cgaaccagtc cgggctcggg gccaccatcg agacgatcgt caggaggggg
     3361 aagtcctcgt gcgcctcgaa ctcgagcgtc accgagccgg gcgagagggc gatcccgccg
     3421 cccgagagct cggacagcgc ggtgccgtct gccagggcgt cggccacctc ggcgagcagg
     3481 agcgtcttcg agccgagctc cgccatggcc tcgattccgg gcgacgcctc ctcccccggc
     3541 gcccagaagc tcacgcgcgc gtcgtggacg gcgccgacga gacccgagaa g
//
LOCUS       DBNHQZ010000231         8858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_474127, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000231 DBNHQZ010000000
VERSION     DBNHQZ010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_474127"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="AC16YU_06130"
     CDS             complement(<1..753)
                     /locus_tag="AC16YU_06130"
                     /inference="COORDINATES: protein motif:HMM:NF052684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR6064835.1"
                     /translation="MTPFFKRSIFPATIAALLLAVSACGGDAGDGHEHDPAAATTAEH
                     DHAAMQDTGHAAAGMLPDLTGGTAALGDGRTLYTCGMHPQVISEEPGNCPICGMRLVP
                     VNPTARGGEVVEIDPGTIQTIGVRTTTVTVAPLTRIVRATGRFVMDEKGAHTVTLKTG
                     GWIERLHVDFEGAIVRKGQPMLELYSPELVTTQQEYLLALATAERMRGTTAAGAEQDA
                     QRVIDAARRRLAYFDLTEEQIARIESTGEVTRT"
     gene            complement(761..2032)
                     /locus_tag="AC16YU_06135"
     CDS             complement(761..2032)
                     /locus_tag="AC16YU_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6064836.1"
                     /translation="MALPNFPGLLVAASLLAAGTAASAQPIERPPEPLLLADVLARVR
                     EANPALSARRLEAAAARTRGDQVSAWRDPMVSATVNTGPGRMQRSEIMVEQAIPWPDV
                     LRARGDAADAAAASLDHSADAALAILLLEATTAYVEAAGAELMIGQIDHFREELAGFE
                     QAAASQYESGLGPQQAILRMQLEKNGLLRRRLELAARIESLRQALVRLAGGEPLAGWA
                     DRPLVLPEVPRAGASPPDSSAARRRPEWGAYEASLRKIMSEQAAARKAFRPDFSVHAS
                     WMEGSIMRGGQDAFGVGIGLRVPLWRAPRHAAVEEKALDGQRVAALMEDLENRTLAEA
                     RAARIRIGSLEETVDLVDGVLLPQAEATQQATAAAYTNGRADYLDLLEAERVLLDLRL
                     DRIALRVRLLTEHATLDRLVPPLGGEPSFDR"
     gene            complement(2122..2595)
                     /locus_tag="AC16YU_06140"
     CDS             complement(2122..2595)
                     /locus_tag="AC16YU_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064837.1"
                     /translation="MLSRFHHSIVAVIVLAALPGAWMPLHLGCEHHGAATAEHASADT
                     ATHHGHGSAPAAAGGHCGKAMPLPPADRDVQLDQVECCDSHGFVAVCCTDRESIPVVI
                     SRTADTPRAQEAAHAPVHVVPGIDLPLPPGRAVVGGGDPPPPARHRYRDLSVLLL"
     gene            2727..2990
                     /locus_tag="AC16YU_06145"
     CDS             2727..2990
                     /locus_tag="AC16YU_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HIR6064838.1"
                     /translation="MGDEGMNRFQKLLDDQHAWPAEYTFKFIVPEDRLDELKAVFGQI
                     PVSVRPSRHGRYMSVTATMLIHSSDEIVAVYTEAGRIPGLIAL"
     gene            complement(3039..3695)
                     /locus_tag="AC16YU_06150"
     CDS             complement(3039..3695)
                     /locus_tag="AC16YU_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6064839.1"
                     /translation="MKRFIVVGLGNFGSTVARELHRQGHDVIAVDLREALVDGIADEV
                     GRAVVADGTSAETLRRAGAEEADVGIVSTGDDVAASVLAVLALIDLGVKDVVAKAVSM
                     EHARILKRIGAGETVFPERDSAVDLATRLSESMLLNYVRLGGGFSVQEMLVPDEWEGM
                     TLRSLELPRKHHISVIGVHDVLTDRITVPPDPDAALKQSDTMIVAGTREALHRIARMH
                     "
     gene            complement(3701..5146)
                     /locus_tag="AC16YU_06155"
     CDS             complement(3701..5146)
                     /locus_tag="AC16YU_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR6064840.1"
                     /translation="MNVLSSLLGALAAGSERLAARWRGLAPARMFALAFAALIGLGTI
                     GFQVLPGLYAGAEMTWLEGLFTATSAVCVTGLIVQDTATFFTPFGQLYVLVLIQLGGL
                     GVVTFATWILIALGQRIPVRQEGLSVDPGSPFSIQPRRLVRSIVRYTLAIEAVGAALL
                     WVLLLPAYGPARGAWHAVFQAISAFCNAGFSTFSDSLVSFSGRPAVLLVIMALIVAGG
                     IGFLTLSEVRRAVTDRRFFLATRAEVRGMRSPLTAHTRIVLASTAILLVTGGVLFTWM
                     EWKGTLAAMSVVDRLVNGLFMSVTARTAGFNSIDYAEASDSSNFLTILLMTVGGSPGS
                     TAGGIKTTTMALLVLLAVARLRGRETVNVGNRSIPNSTVQRSVGLAVLVFAVLATSVF
                     VMSAVEVGITARSGPSGRFLDVLFEATSALNTVGLSLGATSDLSTTGRWITILLMFVG
                     RVGPLTFVSALSRSGSGDHKMRLAQQDVLIG"
     gene            complement(5168..6421)
                     /locus_tag="AC16YU_06160"
     CDS             complement(5168..6421)
                     /locus_tag="AC16YU_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIR6064841.1"
                     /translation="MPLDIDKVRASFPSLFVKDDGAPRIYFDNPGGTQVPRQVIDRTS
                     EYFTRWNANSGGPFATSVQTDRILDDAHASMADLVGAASPDEIVFGQNMTSLTFAMSR
                     TLGRRFRAGDRILVTRMDHDANVAPWLLLARDLGLEVDWLPFSTRTFRYEMERLDELV
                     GERTVLAAVNYASNALGTINDVAGIARRVHAVGGLVYVDAVQYVPHGVTDVRGLGCDF
                     LACSAYKFFGPHQGILWGREELLGNLAAYKVRPASDDLPVRHETGTLSHEGMAGTAGA
                     VDYLAWIGTTMTGADPADRRSAIVAAMTAVAAYERELCRHLVEGLGRIPGLRIHGITD
                     PREFADRVPTVVFEIEGHRPRDLNLALAAEGIFAWDGNYYALEVMQSLGFEGRGGLLR
                     VGLAHYNTEAEVDRLLDVLSHAARN"
     gene            6590..7843
                     /locus_tag="AC16YU_06165"
     CDS             6590..7843
                     /locus_tag="AC16YU_06165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6064842.1"
                     /translation="MRRLALFVVLLLSAPAADARQRTLLVPDRVFDGEAVHEGWVVLV
                     DGERIAAVGDRQGIGAPAGATVLDLPGTTLMPGLIEGHAHILLHPYDETSWNEQVLVE
                     SEAERAIRAAVHVQRTLAAGFTTVRDLGTEGAGYADVGVKQAISKGVIPGPRLLVAGP
                     AIVSTGSYGPKGFHAGVTVPLGAEPADAGDLVRVVRDQVGKGADVVKVYADYRWGPAG
                     EAMPTFSEAELRTIVETAASSGRPVSAHASTPEGMRRATLAGVATIEHGDGGTEEVFR
                     LMAERGVALFATLAAGDAITQYGGWRKGVDPEPARIVAKRRSFALARDAGVVIGMGGD
                     VGVYDHGDNADEMDLMVEYGMSPIDVLRATTSVNADVLGLADRGRIRAGLLADLVAVR
                     GDPTVGLSAVRDVALVMLGGAVVGK"
     gene            complement(7864..>8858)
                     /locus_tag="AC16YU_06170"
     CDS             complement(7864..>8858)
                     /locus_tag="AC16YU_06170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR6064843.1"
                     /translation="IRAGQLIILKSTTYPDTTEGIVQPIIEAEAKKKGLVLGKDWFLA
                     FSPERIDPGNRQFTTANTPVVVGGVTPNCTDVAAEAMGQVVDSVHRVSGPKVAEMEKL
                     LENIFRSVNIALVNELARLCDRIGGVSMWEVIEAASTKPFGFMPFHPGPGLGGHCIPI
                     DPYYLSWLARRYDFETSFITLSARTNEEMPYYVVDGLLKAVADRPLRLTDARVLVLGV
                     AFKRNVDDTRHTPAEKVIEILLEKGVTHVTVSDPHVPEFTAETRHEARRFVSEPLTRE
                     LLEGHDVALILTDHDAFDYELIARHSPLVIDTRNAMARVEHDPARVVLLGGGHF"
BASE COUNT         1346 a         3022 c         3189 g         1301 t
ORIGIN      
        1 cgtccgcgtc acctcgccgg tcgattcgat ccgggcgatc tgttcctcgg tcaggtcgaa
       61 gtaggcgagt cgccggcggg ccgcgtcgat cacccgctgc gcgtcctgct ccgcgcccgc
      121 ggccgtcgtg ccgcgcatcc gctcggcggt ggcgagcgcg agcaggtact cctgctgggt
      181 ggtgacgagc tccgggctgt agagctcgag catgggctga cccttccgga caatggctcc
      241 ctcgaaatcg acgtgcaggc gctcgatcca gccgccggtc ttgagcgtca ccgtgtgggc
      301 ccccttctcg tccatcacga agcggcccgt cgcgcggacg atccgggtga ggggggccac
      361 cgtgaccgtc gtcgtcctca cgccgatggt ctggatcgtc ccggggtcga tctcgacgac
      421 ctcgccgccc cgggcggtcg ggttgacggg caccaggcgc atgccgcaga tcgggcagtt
      481 gcccggctcc tccgagatga cctgtgggtg catgccgcag gtgtacagcg tcctgccgtc
      541 gccgagcgcg gccgtaccgc cggtaaggtc ggggagcatc ccggcggccg cgtggccggt
      601 gtcctgcatg gcggcgtggt cgtgctccgc ggtcgtggcg gccgcggggt cgtgctcgtg
      661 gccgtcgccc gcgtcgcccc cgcaggccga cacggcgagc agcagggcgg cgatcgtcgc
      721 cggaaagatg gaacgcttga agaagggggt catgacgcaa tcaccggtcg aaggacggct
      781 cgccgccgag gggcgggacg agccggtcca gggttgcatg ctccgtgaga agccggacgc
      841 gcagcgcgat ccggtccagc cggaggtcca ggaggacccg ctccgcctcg agcaggtcca
      901 ggtagtcggc gcggccgttg gtgtatgccg ccgcagtcgc ctgttgggtg gcttcggcct
      961 ggggcaggag gaccccgtcg acgaggtcca ccgtctcctc caggctgccg atccggattc
     1021 gggcggcccg ggcttccgcg agggtccggt tttccaggtc ctccatgagg gccgccacgc
     1081 gctgtccgtc gagcgccttt tcctcgaccg ccgcgtgccg gggagcccgc cagaggggaa
     1141 cgcgcaggcc gatccccaca ccgaacgcgt cctggccgcc gcgcatgatc gagccctcca
     1201 tccagctcgc gtgcacggag aagtccggac ggaaggcctt ccgcgccgcg gcctgctcgg
     1261 acatgatctt ccggagcgag gcctcgtacg ccccccactc gggacggcga cgcgcagccg
     1321 aggaatcggg cggcgaagcg cctgcccgtg ggacttccgg cagcacgagc ggccggtcgg
     1381 cccagcctgc caggggctcg cctccggcca gccggaccag cgcctggcgc agcgattcga
     1441 tccgcgcggc gagctccagc cgtcggcgca ggaggccgtt cttctcgagt tgcatgcgca
     1501 ggatggcctg ctggggtccc aggcccgact cgtactggct cgcagcagcc tgctcgaacc
     1561 cggccagctc ctcgcggaag tgatcgatct ggccgatcat gagctcggcg ccggcggcct
     1621 ccacgtacgc ggtcgtggct tccaggagca ggatggcgag cgcggcgtcc gccgagtggt
     1681 ccagcgatgc ggcggcggcg tctgcggcgt ccccgcgggc gcggagcacg tcaggccagg
     1741 gaatggcctg ttcgaccatg atctcggagc gctgcatgcg tcccgggccc gtgttcacgg
     1801 tcgccgagac catcgggtcc cgccaggcgg agacctggtc gccccgggtc cgcgccgcgg
     1861 ccgcctcgag gcgccgggcc gacagggcgg ggttcgcctc ccggacgcga gcgagcacgt
     1921 cggcaagaag caggggttcg ggcggccgct cgatcggctg ggccgatgca gcggttccgg
     1981 ccgcgaggag cgaggctgcg acgagcagcc ccggaaaatt gggcagagcc atgaatccgt
     2041 acgggatgca gaaacgggcg gaagctcggg ccgcgggggc gacccttgac ggccgtcagc
     2101 ggggacggct acgctcgtgc atcacaacag gagtacggac aggtctctgt acctgtggcg
     2161 cgcgggtggc ggcggatcac ctccgcccac cacggctcgg ccgggcggaa gggggaggtc
     2221 gatgcccggg actacatgga ccggcgcatg cgcagcctcc tgcgcccgcg gggtgtcggc
     2281 ggtccggctg atcacgacgg ggatggactc ccggtcagtg cagcacacgg ccacgaatcc
     2341 gtgcgaatcg cagcattcca cctggtccag ctggacatcg cgatcggcgg gaggaagcgg
     2401 catcgccttc ccgcagtgac cgccggccgc cgccggagcc gagccgtggc cgtggtgcgt
     2461 cgcggtgtcc gcggaggcgt gctccgcggt agccgccccg tgatgctcgc atccgaggtg
     2521 aaggggcatc catgcgcccg gaagggcggc gaggacgatc acggccacga tgctatggtg
     2581 gaatcggctg agcacgcgcc attcccaccc ggctggctgc cgggtgttcc ccgcgtatcg
     2641 gacggggaaa cctgaccgat accccctccg ttcggtctgc ttcggaaatt caacggacca
     2701 ccggtcccga accgcgggtg gcgagcatgg gcgacgaggg catgaaccgg ttccagaagc
     2761 tgctggacga ccagcacgcc tggcccgcgg agtacacgtt caagttcatc gtcccggagg
     2821 accggctgga cgagctcaag gccgtgttcg gccagatccc cgtcagcgtg cgcccgtcgc
     2881 ggcacgggcg atacatgagc gtgacggcca cgatgctcat ccactcttcc gacgagatcg
     2941 tggccgtcta cacggaagcg ggccgcatcc ccgggctcat cgcgctctga acgcgccgcg
     3001 cccggccgga cccgtgggcg gtcggaccga ggccgcgctc agtgcatgcg ggcgatccgg
     3061 tgcagcgcct cccgggttcc cgccacgatc atcgtgtcgc tctgcttgag cgcggcgtcg
     3121 gggtccgggg gaacggtgat ccggtcggtg aggacgtcgt ggacaccgat cacggagatg
     3181 tggtgcttcc tggggagctc caggctgcgg agcgtcatgc cctcccactc gtccggaacc
     3241 agcatctcct ggaccgagaa tccgccgccg agccgcacgt aattcaggag catcgattcg
     3301 gacaggcggg tcgccaggtc cacggcggag tcccgctccg ggaacaccgt ctccccggca
     3361 ccgatgcgct tgaggatgcg ggcatgctcc atcgacaccg ccttggcgac gacgtccttc
     3421 accccgaggt cgatcagggc aagcaccgcc agcacgctcg cggccacgtc gtcgcccgtc
     3481 gaaacgatgc ccacatccgc ctcctcggct ccggcgcggc gcagggtctc ggcactggtg
     3541 ccgtcggcga ccaccgcccg gcccacctcg tcggcgatcc cgtccacgag ggcttcgcgg
     3601 aggtccacgg cgatgacgtc gtgcccctgc cgatggagct cgcgggcgac cgtcgagccg
     3661 aagttgccga gacccacgac gatgaagcgt ttcatggcgg ttacccgatg agcacgtcct
     3721 gctgcgcgag acgcatcttg tggtcgccgg atccggagcg ggacagcgcg gagacgaacg
     3781 tgagcggtcc cacccgtccg acgaacatga ggaggatggt gatccaccgc ccggtggtcg
     3841 agaggtcgga cgtcgcgccc aggctcagcc ccaccgtgtt gagggcgctc gtggcctcga
     3901 agagcacgtc caggaatcgc ccggagggcc ccgatcgggc cgtgattccc acctcgaccg
     3961 ccgacatgac gaaaaccgag gtggcgagca cggcgaaaac gaggacggcg aggccgacgg
     4021 agcgctgcac cgtggaattc ggaatgctgc ggttgcccac gttgacggtc tcccggccgc
     4081 ggagacgggc caccgcgagg agcacgagca gcgccatcgt ggtggtcttg atgccgccgg
     4141 ccgtcgagcc cggcgaaccg ccgaccgtca tcagcaggat ggtgaggaag ttcgagctgt
     4201 cggacgcctc cgcgtaatcg atcgagttga agcccgcggt gcgggccgtc acgctcatga
     4261 acaggccgtt caccagccgg tccaccacgg acatggcggc cagggtgcct ttccactcca
     4321 tccaggtgaa gagcactccg cccgtgacga gcaggatggc ggtcgacgcc aggacgatcc
     4381 gtgtgtgggc cgtgagcggc gagcgcatcc cgcggacctc cgccctcgtc gcaaggaaga
     4441 acctccggtc cgtcacggcc cggcgcacct cgctgagcgt caggaaaccg atgccgccgg
     4501 ccacgatcag ggccatgatg acgagcaaca cggcgggacg tccgctgaac gagaccaggg
     4561 aatcgctgaa ggtggaaaaa ccggcattgc agaaggcgct gatcgcctgg aacaccgcat
     4621 gccaggctcc gcgcgccggg ccgtacgcgg gaagcagaag cacccacagc agcgcggctc
     4681 cgaccgcctc gatcgccagc gtgtagcgga cgatgctccg gaccaggcgt cgcggctgga
     4741 tggaaaacgg gcttcccgga tccacggaga gcccttcctg ccgcaccggg atcctctgtc
     4801 ccagggcgat gagaatccac gtggcgaacg tcacgacgcc caggccgccg agctggatca
     4861 gcacgagcac gtagagctga ccgaacggcg tgaagaaggt ggccgtgtcc tggacgatga
     4921 gccccgtgac gcagaccgcg ctcgtggccg tgaacagtcc ctcgagccag gtcatctccg
     4981 ctccggcata gaggcccgga agcacctgga agccgatcgt accaaggccg atgagcgccg
     5041 cgaaggcgag cgcgaacatc cttgcaggcg ccaggccacg ccaccgggcg gcgagccgct
     5101 ccgaaccggc cgcgagcgcg ccgagcaggc tactaaggac attcattcgg tcttcaggcg
     5161 tccgtgctca gtttcgggcg gcatgcgaaa gtacgtccag gaggcgatcc acttcggctt
     5221 ccgtgttgta atgcgcgagc ccgacgcgaa gcagtccgcc ccgcccctcg aagccgaggc
     5281 tctgcatgac ctcgagggcg tagtagttgc cgtcccacgc gaagatcccc tcggcggcga
     5341 gggccaggtt caggtcacgc ggccggtggc cctcgatctc gaacacgacc gtgggcacgc
     5401 ggtccgcgaa ttcacgcggg tccgtgatgc cgtggatgcg caggcccggg atgcggccga
     5461 ggccctcgac gaggtggcgg cacagttcgc gctcgtacgc ggcgacggcc gtcatcgcgg
     5521 cgacgatcgc gctccggcgg tccgccgggt ccgcgccggt catcgtggtc ccgatccagg
     5581 ccaggtagtc gaccgccccc gccgttccgg ccatcccttc gtgcgagagc gtcccggtct
     5641 cgtgccgcac agggaggtcg tcggatgccg ggcgcacctt gtaggccgcc aggttgccga
     5701 gcagctcctc gcgtccccac aggatcccct ggtgcggccc gaagaacttg tacgccgagc
     5761 acgccaggaa atcgcagccc aggccgcgga cgtcggtcac cccgtgcggg acgtactgca
     5821 cggcgtccac gtagacgagg ccgcccactg cgtgcacccg gcgggcgatc ccggccacgt
     5881 cgttgatggt cccgagcgca ttcgaggcgt agttcacggc cgcgagcacg gtgcgctcgc
     5941 cgaccagctc gtcgagccgc tccatttcgt agcggaaggt ccgcgtcgag aacggcagcc
     6001 agtccacctc cagacccaag tcgcgggcca gcagcagcca cggggcgacg ttcgcgtcgt
     6061 ggtccatgcg ggtgaccagg atccggtccc ccgcgcggaa gcgccggccc agggtgcggg
     6121 acatcgcgaa ggtgagcgac gtcatgttct ggccgaacac gatttcgtcg ggcgatgcgg
     6181 cgccgacgag gtccgccatc gacgcatggg cgtcgtccag gatgcggtcg gtctggaccg
     6241 acgtcgcaaa aggtccgccc gagttcgcgt tccagcgcgt gaagtactcg ctcgtccggt
     6301 cgatgacctg gcgcggcacc tgggtgccgc cggggttgtc gaagtagatg cggggcgcgc
     6361 cgtcgtcctt cacgaagagg gaagggaagg atgcgcgaac cttgtcgatg tccagcggca
     6421 tgggcttcgg tgtgaaccgt gcgcggggaa gctacgatgc ggccggttcc ctatcgcgcc
     6481 gcgggtcgcg accgcggttg cggcgcggcc gcggaagcgt cgcgatcgag gttgcggcga
     6541 agcgggcgcc ccggtatcct cgctccgtcc atcagcccgg gttgccgcca tgcgccgcct
     6601 cgccctgttc gtggtcctgc tcctctccgc gccggctgcc gacgcgcgcc agcggacgct
     6661 cctcgtgccc gaccgtgtgt tcgacgggga ggccgtgcac gagggctggg tcgtgctcgt
     6721 ggacggcgag cggatcgccg cggtcggcga tcggcagggc atcggggctc cggccggtgc
     6781 gaccgtcctg gatcttcccg gaacgaccct catgcccggc ctcatcgagg ggcacgccca
     6841 cattctgctc cacccgtacg acgagacctc ctggaacgag caggtactcg tcgagtcgga
     6901 ggccgaaagg gcgatccggg ccgcggtgca cgtgcagcgg acgctcgcgg ccgggttcac
     6961 gaccgtgcgg gacctgggga ccgaaggggc cggctacgcg gatgtcgggg tcaagcaggc
     7021 gatctcgaag ggggtgattc ccggcccgag gctccttgtg gcgggtccgg ccatcgtgtc
     7081 cacgggttcg tacggaccga agggcttcca tgccggagtg acggttccgc tcggcgccga
     7141 gccggcggac gccggcgacc tggtccgcgt ggtccgcgac caggtcggga agggggcgga
     7201 cgtcgtgaag gtgtacgccg actaccggtg ggggccggcg ggcgaggcca tgcccacctt
     7261 ctccgaggcc gagttgcgga ccatcgtcga gaccgcggcc agctcgggcc ggccggtatc
     7321 ggcccacgca tcgacgcccg agggtatgcg ccgggcgacg ctcgccggcg tcgccaccat
     7381 cgagcacggc gacggcggca cggaggaggt gttccgcctg atggccgagc gcggtgtcgc
     7441 gctcttcgcg acgctggcgg ccggagacgc catcacgcag tacgggggat ggaggaaggg
     7501 cgtcgatccc gagcccgcgc gcatcgtggc caagcggcgt tcgttcgccc tcgctcgcga
     7561 cgcgggagtc gtgatcggca tgggaggcga cgtgggcgtc tacgaccacg gcgacaatgc
     7621 cgacgaaatg gacctcatgg tcgagtacgg catgtcgccg atcgacgtcc tgcgggccac
     7681 gacgtcggtg aacgccgatg tcctgggcct cgcggaccgc ggacgcatcc gcgcggggct
     7741 gctcgcggac ctcgtggcgg ttcgggggga tccgaccgtc gggctgtcgg ccgtgcgcga
     7801 cgtggccctc gtgatgctgg gcggtgcggt ggtcgggaag tgagggtgcc tgcagagggc
     7861 ccttcagaaa tgcccgccgc ctagcagcac caccctcgcc gggtcgtgct cgacgcgcgc
     7921 catggcattg cgcgtatcga tcacgagcgg gctgtgccgg gcgatgagct cgtaatcgaa
     7981 cgcgtcgtga tcggtcagga tgagcgccac gtcgtggcct tcgagcagct cgcgagtgag
     8041 cggctccgac acgaagcggc gcgcctcgtg ccgagtctcc gccgtgaact ccggcacgtg
     8101 cgggtcgctc acggtgacgt gggtgacgcc cttctccagg agaatctcga tgaccttttc
     8161 ggcgggggtg tgccgggtgt cgtccacgtt ccgcttgaag gcgacgccga gcaccagcac
     8221 gcgggcgtcg gtgagccgga gcggacgatc cgcgaccgcc ttgagcagcc cgtccaccac
     8281 gtagtacggc atctcctcgt tcgtacgcgc ggaaagcgtg atgaagctcg tttcgaagtc
     8341 gtagcggcgc gccagccacg acaggtagta cgggtcgatc gggatgcagt gcccccccag
     8401 gccggggccc gggtggaagg gcatgaagcc gaacggcttg gtcgacgcgg cctcgatgac
     8461 ctcccacatg gagacgcccc cgatccggtc gcagagccgc gcgagctcgt tcaccagcgc
     8521 gatgttcacc gagcggaaga tgttctcgag cagcttctcc atctcggcaa ccttcggccc
     8581 ggagacccgg tgcacggagt cgaccacctg tcccatggcc tcggcggcca cgtcggtgca
     8641 gttcggcgtg acgcccccga ccacgacggg ggtgttggcc gtcgtgaact gacggttgcc
     8701 cgggtcgatg cgctccgggc tgaacgccag gaaccagtcc ttacccagca ccaggccctt
     8761 tttcttggcc tcagcctcga tgatcggctg cacgatgccc tcggtggtat ccggatacgt
     8821 cgtactctta aggataatga gctgcccggc gcggatat
//
LOCUS       DBNHQZ010000232         6548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2100273, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000232 DBNHQZ010000000
VERSION     DBNHQZ010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2100273"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="AC16YU_06175"
     CDS             <1..1938
                     /locus_tag="AC16YU_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064844.1"
                     /translation="LAPHALFELGVVQYGARQWESANATFGRLVESYPESDLAGSALT
                     YLGNTFIALGDFDSALDAFDRAISYEAGDPARRDEVRFQKAWLLYRSESYADAEQAFL
                     DLYTADPSGPQGEASLFWSAESAFQGDRLAAAADRFVRYMRDFPGGRQMDAAHYALGW
                     TYFRQQRYDLAVVEFQQFLSGYREATESVPYRTDAELRLGDSYYALKDYANAVRVYGR
                     LAADGDDYALYQIGQAYANSGDPIEAIASFRRLLEEFPDSEWREEARYSLGYLFFQNQ
                     EYDQAITEYRTLIRDYGSHPLAAKAQYGIGDAYFNAGSLDRAIAEYRAVLERYPNSPF
                     AVDAAGSIQVALVAAGDEERAVAIVDSFAAAHPDSPLVDELRFRQAEVLYRSGRTDEA
                     RDDLLVFVRTARHEELLPEAYYYLGVIYADRGQAREAATYLGQILDRYPDSPRRAEAA
                     AQLGRLYLESGNLDRALDAYETLEGVSQDDPVRHAEALYGRSMTLLAQGRMDDAERIL
                     MELVEDAPDDPSTAPAHLGLGRILEMHGEMNRAVTRYRFAATRSQGEAGAEALYRLGS
                     LLRRTGDPSGAIEELTRIPVRFAGYTEWMARGYLEQARSLRDMGRAGEAVRLYETLIS
                     SYPQHPTAEEARRERAELVED"
     gene            1941..3674
                     /locus_tag="AC16YU_06180"
     CDS             1941..3674
                     /locus_tag="AC16YU_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6064845.1"
                     /translation="MTPKRILPVALFAALLVVPAARAQDEPVLPDLTPREVEIRGELT
                     IRFPSLRRQPLVGFNPPPRIPDIAADRRPFAEPYKQERADLPLSPLRTPTAPAVASYL
                     DGPVFVGQVEGGVGRYTERFVLADLAARPGSAFHWTVDGSYRGRTGFEAYPEAEARYE
                     QGSFSAQGVAPLGGIDVGASMEGVVSNYVLYGVVPTFENLDAPLPERDVRGGMVGASV
                     GRNRGTVRVKVQGALGASSIRTDVAGADDTLQRGAEREDRTASLGGSLETVGQTLGVW
                     LAADYAKTTPVEDSTPSAAADRYSAGGGVKLEVGRVGRLSAGVRVLGVGRDALSALED
                     DRSFSYLSPDVRLDAAIGPNLRLEVSQRPGTTSARLRDLYGEQPYLVDSPVVEPEIWP
                     VDAQGRLVAALGRVELALAAGYRRSPNRRYFEHDPTNVLDFVRGISTARHGKSREADA
                     AVDVIGYFGASVQAGIGATVRSARLTDLDADVPYVAPWTLRSLVSAALLDGRGLVQVT
                     MRVEGPRDRDLAGSLELPAFADLDFLASYRVTRNASVVLRLDNVGGNRPEWDRYLRPS
                     AVYTAGVAWEW"
     gene            3731..5002
                     /locus_tag="AC16YU_06185"
     CDS             3731..5002
                     /locus_tag="AC16YU_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6064846.1"
                     /translation="MPVNPSDFLRALVLVVTERLQRGEDTSLPGVGTWSAPTDPDSGR
                     VMVRFAEGPGGDPASDLSLAREVAAEIGATPAEISPRIEGFARAVQEHLRETGSVLLP
                     GIGRLTGRPGRVAFRAESFFTEAVDWDRPAGPPATVPAEAEEAGSETANAWVVPDDAG
                     RDEEAGGGPEPEPVSDEPWEPSEAYPEFAPPRAPRSEDPVERVAESGYDSRPPMPPAP
                     APSVAARGSRPVARRSRRMPPLPLVIAVLVVAGAAVVIALLRGGGPPETVSRPAPPDT
                     SSVGLTAATPADTSALAIADTALSGAPSGGIAEPVTGTTAETTRSAPPPAEEPTRPAP
                     AVARQDFDTSSSGYTLVVASTVIESDALAGIQRYRQLGLPAAVLAYEDDGSVRYRLAV
                     GLYESAAAADRARQEMADRLPAGTWVRRIRP"
     gene            5029..5769
                     /locus_tag="AC16YU_06190"
     CDS             5029..5769
                     /locus_tag="AC16YU_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6064847.1"
                     /translation="MTLAFLQQVADSAVNPSLLQAIPPPDEVSTLEILLEGGWVMLPI
                     ILLSIVTIYLFVERLMTLRKAATDPAAIMDRIRDYVRAANIDGAQAFCDAQDKPITRI
                     LKHGLERLGRPISEIQDAVNAAGKYEAFELEKRTDLLASIAGIAPMLGFLGTVTGMIS
                     AFQQIQNLQGNVNPSVLAGGIWEALLTTAAGLIVGILAFFFYNFLFERIRRLVNDLER
                     SATEFIDLLQAPAPSSSGASRARIQDSF"
     gene            5779..6192
                     /locus_tag="AC16YU_06195"
     CDS             5779..6192
                     /locus_tag="AC16YU_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6064848.1"
                     /translation="MSSINFSTERKPLTTFSLAGLADIVLLLLIFFLLTSSFIPQFGI
                     RVNLPQIDASAPTDARYVTVAITSDGDYYVEQERTPRDGLLDAVQRVMGDRTALVLRA
                     DQEATVGQFATVAGVAQALNLRVLMATERVDLRPR"
     gene            complement(6200..>6548)
                     /locus_tag="AC16YU_06200"
     CDS             complement(6200..>6548)
                     /locus_tag="AC16YU_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR6064849.1"
                     /translation="DLHVHVEPVHPADWSSTRGEHSGAIAARVAAARERRQGRAARVR
                     GNGRPDHLLEPECEELIARAVDRLELSARATVRIRSVARTIADLAGSDHVRPGHVAEA
                     IQYRSLDRRLIPL"
BASE COUNT          953 a         2382 c         2207 g         1006 t
ORIGIN      
        1 ctggccccgc acgccctgtt cgagctcggc gtcgtgcaat acggcgcccg gcaatgggag
       61 agcgccaacg ccacgttcgg acggctcgtg gaatcgtacc cggaatcgga cctcgcgggc
      121 tccgccctca cctacctcgg caacacgttc atcgccctcg gggacttcga ttcggcgctc
      181 gacgcgttcg accgggccat ctcgtacgaa gccggagacc cggcccgccg ggacgaggtg
      241 cggttccaga aggcctggct gctttaccgc agcgaatcgt acgcggacgc cgagcaggcc
      301 ttcctggacc tgtacacggc cgacccgtcg ggtccccagg gggaggcctc cctgttctgg
      361 tccgcggaaa gcgcgttcca gggcgatcgc ctcgccgccg ccgcggaccg gttcgtccgg
      421 tacatgcgtg acttcccggg aggccggcag atggatgccg cgcactacgc tctcggctgg
      481 acctacttcc gccagcagcg atacgacctg gcggtggtcg aattccagca gttcctgtcc
      541 ggctaccggg aggcgacgga gtccgtgccg taccgcacgg acgcggaact ccgcctcggt
      601 gacagctact acgcgctgaa ggactacgcc aacgccgtgc gcgtgtacgg ccggctcgcc
      661 gcggacgggg acgactacgc gctgtaccag atcgggcagg cgtacgcgaa ttcgggcgac
      721 cccatcgagg ccatcgcgtc cttcaggcgt ctgctcgagg aattcccgga cagcgagtgg
      781 cgcgaggagg cccgctattc gctcggctac ctcttcttcc agaaccagga gtatgaccag
      841 gcgatcacgg agtatcgcac gctcatccgg gactacggca gccatcccct cgccgccaag
      901 gcccagtacg gcatcggcga cgcgtacttc aacgccggca gcctggatcg cgcgatcgcc
      961 gaataccgag cggtcctcga gcgatatccg aacagtccct tcgcggtgga cgcggccggc
     1021 agcatccagg tcgccctcgt tgccgcgggc gacgaggaac gggccgtggc gatcgtcgac
     1081 tcgttcgcgg cagcgcatcc ggactccccg ctggtcgacg agctccggtt tcgccaggcg
     1141 gaagtcctgt accgctcggg acgcacggac gaggcgcggg acgacctgct cgtcttcgtg
     1201 cggaccgccc gccacgagga actcctgccc gaagcctact actacctggg cgtgatctat
     1261 gccgaccggg ggcaggcccg ggaggccgcc acgtacctgg ggcagatcct ggaccggtat
     1321 ccggacagcc ccaggcgtgc cgaggccgct gcccagctgg gacgcctgta cctggagtcc
     1381 ggcaatctgg accgggctct cgacgcctac gagaccctcg aaggggtctc ccaggacgat
     1441 ccggtccggc acgcggaggc cctgtacggg cgaagcatga cgctcctcgc acaggggcgc
     1501 atggacgatg cagagcggat cctcatggaa ctcgtcgaag atgcgccgga cgatccgagc
     1561 acggcgcccg cccacctcgg gctcggccgc atcctcgaga tgcacggcga gatgaacagg
     1621 gcggtgacgc gctaccgttt cgcggcgacg cggagccagg gcgaggccgg ggccgaggcc
     1681 ctgtaccgcc tgggctcgct cctgcgacgg acgggcgatc cttcaggcgc gatcgaggag
     1741 ctcacgcgca tcccggtccg gtttgccgga tacaccgagt ggatggcccg gggctacctg
     1801 gagcaggcgc gatccctgcg cgacatgggt cgggccggcg aagccgtccg cctgtacgag
     1861 accctcatca gcagctaccc ccagcacccg accgccgagg aagcaaggcg tgagcgggcc
     1921 gaactcgtgg aggactgaac atgaccccga agagaatcct cccggtcgcc ctgttcgcgg
     1981 ctctgctcgt cgtgccggcg gcccgcgccc aggatgagcc cgtgctcccg gacctcacgc
     2041 cccgcgaggt cgagatccgc ggcgagctca ccatccgttt cccttcgctc cgcaggcagc
     2101 cgctcgtcgg gttcaatcct cctcccagga ttcccgacat cgcggccgac cggaggccgt
     2161 tcgccgaacc ctacaagcag gaacgcgccg acctgccgct gagccccctg cgtacgccga
     2221 ccgcgccggc cgtcgcctcc tacctggacg gcccggtctt cgtcggacag gtcgagggcg
     2281 gcgtcgggcg ctacacggag cggttcgtac tggccgacct cgcggcccgg cccggctcgg
     2341 cgttccactg gacggtcgac ggatcctatc gtgggcgaac cggcttcgag gcctaccccg
     2401 aggccgaggc ccgctacgag cagggcagct tctccgccca gggcgtcgcg cccctcggag
     2461 ggatcgacgt cggtgcctcg atggagggcg tcgtctcgaa ttacgtgctg tatggcgtcg
     2521 ttccgacgtt cgagaacctg gatgccccgc ttcccgagcg tgatgtgcgt ggaggaatgg
     2581 tcggggcatc ggttggccgg aaccgtggaa cggtgcgcgt gaaggtccag ggagcgctcg
     2641 gcgcttccag catccggacg gacgtggcgg gagcggacga tacccttcag aggggcgccg
     2701 agcgcgagga ccgcaccgcc tccctcggtg gatccctcga gaccgtcggg cagacgctgg
     2761 gcgtgtggct cgcggcagac tatgcgaaga cgaccccggt cgaggattcc acgccgtccg
     2821 ccgccgcgga ccggtactcg gccggcggcg gcgtgaagct cgaggtgggc cgtgtcggac
     2881 gcctgtccgc gggagtccgc gtcctgggcg tcggacgcga cgcactgagc gcgctcgaag
     2941 acgatcggtc cttcagctac ctctcccccg atgtccggct cgacgccgcg atcggtccga
     3001 acctccggct ggaagtatcg cagcgccccg ggacgacgag cgcacggctg cgcgacctgt
     3061 acggggaaca gccgtatctc gtcgattcgc cggtcgtcga gcctgaaatc tggcccgtcg
     3121 atgcccaggg ccgcctcgtg gcagccctcg ggcgcgtcga gctggcactc gccgccgggt
     3181 atcgtcgctc tcccaaccgc cggtatttcg agcatgatcc gacgaatgtc ctcgacttcg
     3241 tccggggcat ttcgaccgcc cgccacggca agtcccgcga ggccgatgcc gcggtggatg
     3301 tgatcgggta cttcggcgcc tcggtgcagg cgggcatcgg ggccaccgtc cggagcgcgc
     3361 gcctgacgga cctcgatgcc gacgtgccgt acgtcgcccc atggacgctg cggagcctcg
     3421 tctccgccgc gctcctcgat ggtcgaggcc tggtgcaggt caccatgcgc gtcgaagggc
     3481 ctcgcgatcg cgacctcgcc ggctcactcg agcttccggc tttcgccgac ctggatttcc
     3541 tggcctccta ccgcgtcacc cggaacgcct cggtcgtcct ccggctcgac aacgtcgggg
     3601 ggaatcgccc ggagtgggac cgatacctga ggccgtccgc cgtctacacc gcgggtgtcg
     3661 catgggaatg gtgaccggtc gaatccggaa gactgtgtcc gtatcttgat tgtttgagcc
     3721 cgaaccgagc gtgcccgtca acccgagcga ctttcttcgc gccctcgtgc tcgtcgtgac
     3781 cgagcggctg caacgtggag aggacacctc cctccccggc gtcggcacct ggtctgcccc
     3841 gaccgacccg gattccgggc gcgtcatggt ccgattcgcc gagggaccgg ggggcgaccc
     3901 ggcttccgac ctgtcgctcg cccgggaggt ggcggccgag atcggggcaa cgccggcgga
     3961 gatctccccc agaatcgagg gcttcgcgcg cgccgtgcag gagcatctgc gggagaccgg
     4021 ttcggtgctc ctgccgggca tcggacgact cacgggcagg cccggcaggg tggcattccg
     4081 cgcggagtct ttcttcacgg aggccgtcga ctgggatcgg ccggccgggc cgcccgcgac
     4141 cgtcccggcc gaggccgagg aagccggttc cgagacagcc aatgcgtggg tagtcccgga
     4201 cgacgccggg cgcgacgagg aagccggggg cgggccggag cccgagcccg tgtcggacga
     4261 gccgtgggag ccttccgagg cgtacccgga gttcgcgccg ccccgagcgc cgaggtcgga
     4321 ggatccggtg gaacgagtcg cggagtcggg ctatgactcg cggcctccca tgcctcccgc
     4381 acccgctccg tccgtcgccg cgcgcgggtc gcggcccgtt gcgcgccgat cccgcaggat
     4441 gccgcccctg ccgctcgtca tcgcggtgct ggtcgtcgcc ggcgctgccg tcgtgatcgc
     4501 tctgctgcgc ggcggcggcc ccccggaaac ggtttcgcgt cccgcgccgc cggacacctc
     4561 gtcggtggga ctcacggcgg ccactcccgc cgataccagc gccctggcca tcgcggacac
     4621 ggccctgtcc ggcgcgccgt caggcggcat cgccgaaccc gtgaccggga ccacggccga
     4681 gaccacgcgg tcggccccgc cgcccgccga ggagccgacc cggccggcgc ccgccgtggc
     4741 gcggcaggac ttcgacactt cttcgtccgg ctacacgctc gtcgtcgcat ccaccgtgat
     4801 cgaatcggat gcccttgcgg gcatccaacg ctaccggcag cttgggctgc ccgccgccgt
     4861 cctcgcgtac gaggacgacg gatccgtccg gtatcggctg gccgtcggcc tgtacgagtc
     4921 cgcggccgcc gcggaccggg cacggcagga gatggcggac cggctcccgg ccggtacgtg
     4981 ggtccggcga atcaggccct gaatccaacc ctttcaccta gtccgtccat gaccctcgcc
     5041 tttctccagc aggtcgccga cagcgccgtg aacccttccc tcctgcaggc catccctccg
     5101 cccgacgagg tctcgaccct cgagatcctg ctggagggcg gctgggtgat gcttccgatc
     5161 atcctcctct cgatcgtgac gatctatctc ttcgtcgagc ggctgatgac gctccggaag
     5221 gccgccacgg atccggcggc gatcatggac cggatccgtg actacgtgcg ggccgcgaac
     5281 atcgacggag cccaggcctt ctgcgacgcc caggacaagc ccatcacccg catcctcaag
     5341 cacggcctcg aacggctcgg gcgaccgatc tcagagatcc aggatgccgt caatgccgcg
     5401 ggcaagtacg aggccttcga gctcgagaag cggaccgacc tcctggcatc catcgccggc
     5461 atcgcgccga tgctgggatt cctcgggacg gtgaccggca tgatctcggc gttccagcag
     5521 atccagaacc tccagggcaa cgtcaatccc agcgtgcttg ccggcggcat ctgggaggca
     5581 ctcctcacga cggcggccgg cctgatcgtc ggcatcctgg cgttcttctt ctacaacttc
     5641 ctcttcgagc gcatccggcg cctcgtgaac gacctcgaac gctcggccac cgagttcatc
     5701 gacctgctcc aggcgcccgc gccgtcgagc tccggcgcct cgcgcgcccg catccaggac
     5761 tcgttctagg tctgcaccat gtcatccatc aatttctcca cggaacgcaa gcccctgacg
     5821 acgttcagcc tggccgggct cgccgacatc gtgctgctgc tgctcatctt cttcctgctc
     5881 acgtccagct tcatcccgca gttcggcatc cgggtgaacc tcccgcagat cgatgcctcc
     5941 gcgccgacgg atgcgcggta cgtgacggtg gcgatcacgt ccgacggcga ctactacgtc
     6001 gagcaggagc gcacgcctcg ggatgggctg ctcgacgcgg tgcagcgcgt catgggcgac
     6061 cgtacggcgc tcgtgctccg ggccgaccag gaggcaaccg tcggccagtt cgcgacggtg
     6121 gcgggcgtgg cccaggcgct gaacctccgc gttctcatgg cgaccgagcg cgtggacctg
     6181 cgtccccgct gattgccccc tacaggggaa tgagcctccg gtcgagactc cggtactgga
     6241 tggcttccgc cacgtgcccg gggcgcacgt ggtcggaccc ggcaaggtcg gcaatcgtcc
     6301 gcgccaccga acgaatccgc accgtcgcgc gcgccgagag ctccaggcga tccacggccc
     6361 gggcgatcaa ttcctcgcat tccggttcca gcaggtggtc cggccggccg ttgccccgca
     6421 cgcgcgcggc gcgcccctgg cgtcgctcgc gcgcggcggc gacccgggca gcgatggccc
     6481 cggagtgctc cccgcgcgtg ctgctccagt ccgcgggatg cacgggctcc acgtgcacgt
     6541 gcaggtcg
//
LOCUS       DBNHQZ010000233         4426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1829338, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000233 DBNHQZ010000000
VERSION     DBNHQZ010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1829338"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1973
                     /locus_tag="AC16YU_06205"
     CDS             51..1973
                     /locus_tag="AC16YU_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HIR6064850.1"
                     /translation="MANPLDSRVPPGPLKDKWDLYKNSIKLVNPANKRRHTVLVVGTG
                     LAGGSAAASLAELGYNVKAFTILDSARRAHSIAAQGGINAAKNYPNDGDTIWRLFYDT
                     IKGGDYRSREANVYRLAQVANSIIDQCVAQGVPFAREYGGLLANRSFGGAQVERTFYA
                     RGQTGQQLLLGAYSAMSRQVATGKIRMFNRREMLDLVVIDGRARGIITRNLVTGEMER
                     HLGDAVLLCTGGYGNVYYLSTNAKNCNVTAAWRAHRRGALFGNPAFTQIHPTCIPVSG
                     EYQSKLTLMSESLRNDGRMWVPKRPGDTRPPTEIPEEERDYFLERRYPSFGNLVPRDV
                     ASRNSKYVCDEGRGVGETQLAVYLDFADSIGRLGRNVIEERYGNLFEMYERITGENPY
                     RVPMRIYPAVHYTMGGLWVDYELQSTIPGLFVLGEANFSDHGGNRLGASALMQGLADG
                     YFIIPYSLGNYIAGSRLEAASTEHDAFEAAESEARDRIEKLLSIRGGRTVTEFHRALG
                     SIMWDHVGMSRTRDGLTTAIDEIAALRDEFWQDVLVPGEPTTYNKNLEFAGRIADFLE
                     LGELMARDALMREESCGGHFREEFQTEDGEALRNDRDFAFVSAWQYNPDDIRRPTMHR
                     EDLVFENVQLTTRSYK"
     gene            2017..2766
                     /locus_tag="AC16YU_06210"
     CDS             2017..2766
                     /locus_tag="AC16YU_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR6064851.1"
                     /translation="MRINLKIWRQPGPDAAGRFETYVVEDANPHMSFLELLDVLNERL
                     IKEGEEPVEFDHDCREGICGSCGLVVNGVAHGPQPKTAVCQLHMRHYRDGDTILVEPW
                     RAKAFPLIRDLVVDRSAFDRIMAAGGYVSVSTGNAPDANSIPISKVDAELAMDYASCI
                     GCGACVAACPNASASLFTAAKIAHLAHLPQGHPERRRRVRGMVEQMEKEGFGDCSNHA
                     ECEAVCPKGISIAAIATMRREYMKAVLSGPA"
     gene            complement(2783..3487)
                     /gene="purQ"
                     /locus_tag="AC16YU_06215"
     CDS             complement(2783..3487)
                     /gene="purQ"
                     /locus_tag="AC16YU_06215"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR6064852.1"
                     /translation="MSASVGVLVFPGSNCDHDAYHVARHVLGLETRFIWHKEEDLGGV
                     DLVVVPGGFSYGDYLRSGAIARFSPIMREVVRFAEGGGLVLGICNGFQILCESGLLPG
                     ALMRNASLRFVCRHVHLRVETGATPFTLGQEGDVVRVPIAHGDGNYYADPETLDRLER
                     EDRVVFRYCDGDGHVDESANPNGSSRNVAGVLSKGRNVLGMMPHPERCSEAILGRPDG
                     IRLFESVVRHLAGVPA"
     gene            complement(3583..4197)
                     /locus_tag="AC16YU_06220"
     CDS             complement(3583..4197)
                     /locus_tag="AC16YU_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR6064853.1"
                     /translation="MTHILHAAALPAVIAFSAPGIHGGTDTTAVDVPTYSVDEAHSSI
                     GFKVRHLGITNVNGRFADFDATVRMDPDDLSTLEATATIQVGSVDTGVEKRDGHLKSD
                     DFFDAANHPVITFVSKGVRNVEGDEFELVGDLTIRGTTREVVLDGTYLGSASMGESQR
                     VAIEASTEISRFDYGLKWDAVTELGGLIVSEKVRILLEIQAVQD"
BASE COUNT          780 a         1491 c         1463 g          692 t
ORIGIN      
        1 gggtctagac gccgcgatca cccgcgttcg atctccagac gaattccgtc atggccaatc
       61 ctctcgattc aagggtcccc ccaggtccac tgaaggacaa atgggatctg tacaagaact
      121 cgatcaagct ggtcaatccg gccaacaagc gcaggcacac cgtcctcgtg gtgggcacgg
      181 gcctcgccgg cggatcggcg gccgcctcgc tggcggagct cggatacaac gtgaaggcgt
      241 tcacgattct ggattccgcc cggcgcgcgc actcgatcgc cgcccagggc ggcatcaacg
      301 ccgccaagaa ctaccccaac gacggcgaca cgatctggcg cctgttctac gacacgatca
      361 agggcggcga ctaccgctcg cgggaagcga acgtgtaccg gctcgcccag gtcgcgaaca
      421 gcatcatcga ccaatgcgtg gcccagggcg tcccgttcgc gcgcgagtac ggcgggctgc
      481 tcgccaaccg ctcgttcggg ggcgcccagg tcgagcggac cttctacgcc cgcggccaga
      541 cgggccagca gctgctgctc ggcgcgtatt cggccatgag ccggcaggtc gcgacgggca
      601 agatccggat gttcaaccgc cgcgagatgc tggacctcgt cgtgatcgac ggccgggccc
      661 gcggcatcat cacccggaac ctggtgaccg gcgaaatgga gcggcacctg ggcgacgcgg
      721 tccttctgtg cacgggcggt tacggcaacg tgtactacct gtccacgaac gccaagaact
      781 gcaacgtcac cgccgcctgg cgcgcgcacc ggcgcggcgc cctgttcggc aacccggcct
      841 tcacgcagat ccatcccacg tgcatcccgg tgtcgggcga gtaccagtcc aagctcacgc
      901 tcatgagcga gagcctgcgc aacgacggac ggatgtgggt gcccaagcgg ccgggcgaca
      961 cgcggccgcc gaccgagatt ccggaggagg agcgcgacta cttcctggag cgccgttacc
     1021 cgagcttcgg caacctggtg ccccgcgacg tggcgagccg caactccaag tacgtgtgcg
     1081 acgagggacg cggcgtcggg gaaacgcagc tcgcggtcta cctggacttc gcggattcca
     1141 tcgggcgcct cggccggaac gtgatcgagg agcgctacgg caacctgttc gagatgtacg
     1201 agcgcatcac gggcgagaat ccgtaccggg tgccgatgcg catctacccg gccgtgcact
     1261 acacgatggg cggcctgtgg gtggactacg agctgcagtc gacgatcccc ggccttttcg
     1321 tgctcggtga agccaatttc tcggaccacg gaggaaatcg cctcggcgcc tcggccctca
     1381 tgcagggcct ggccgacggg tatttcatca tcccctactc gctcggcaac tacatcgccg
     1441 ggagccgcct ggaggcagcc tccaccgagc acgacgcgtt cgaggccgcg gagagcgagg
     1501 cccgggaccg catcgagaag ctgctctcga tcaggggcgg ccggacggtg acggagttcc
     1561 accgcgcgct cggaagcatc atgtgggacc acgtgggcat gtcgcgcact cgcgacgggc
     1621 tcaccacggc catcgacgag atcgcggcgc tccgggacga gttctggcag gatgtcctgg
     1681 ttccgggcga accgaccacc tacaacaaga acctggagtt cgccgggcgg atcgccgact
     1741 tcctggagct cggcgagctc atggcccgcg atgccctgat gcgcgaggag tcgtgtggcg
     1801 ggcacttccg cgaggagttc cagaccgagg acggcgaggc actccggaac gacagggatt
     1861 tcgccttcgt ctccgcctgg cagtacaacc cggacgatat ccgccgaccc acgatgcacc
     1921 gggaggacct cgtcttcgag aacgtccagc tgaccacccg cagctacaag taggacgccc
     1981 gagcggcaca agaatcaggc atccatggca cattcgatgc gcatcaacct gaagatctgg
     2041 cgccagccgg gtcccgacgc ggccggccgg ttcgagacct acgtggtcga ggacgccaac
     2101 ccgcacatgt ccttcctgga gctgctcgac gtcctgaacg agcggctgat caaggagggc
     2161 gaggagccgg tcgaattcga tcacgactgc cgcgagggca tctgcggctc ctgcggactg
     2221 gtcgtgaacg gcgtagccca cgggccccag ccgaagacgg ccgtctgcca gctgcacatg
     2281 cggcactatc gcgacggcga caccattctc gtcgagccgt ggcgggcgaa ggccttcccg
     2341 ctcatccgtg acctggtcgt ggaccgatcg gcgttcgacc ggatcatggc ggcgggcgga
     2401 tacgtgtcgg tgagcaccgg gaacgcgccc gacgcgaact cgatacccat cagcaaggtg
     2461 gacgccgaac tcgcgatgga ctacgcatcc tgcatcggct gcggggcgtg cgtagcggcg
     2521 tgcccgaacg cgtcggcgtc gctgttcacg gcagcgaaga tcgcgcacct cgcccacctg
     2581 ccccagggcc atccggagcg gcgcagacgg gtgcgcggca tggtcgagca gatggagaag
     2641 gagggcttcg gcgactgctc caatcacgcg gagtgcgagg ccgtctgccc gaaggggatc
     2701 tcgatcgcgg cgatcgcgac catgcgccgc gagtacatga aggccgtcct ctccggcccc
     2761 gcgtgacgcg gcgtccgcgc cgtcaggccg gaaccccggc caggtgccgg accacggact
     2821 cgaacaggcg gatgccgtcg ggcctcccca ggatggcctc ggagcagcgc tccgggtgcg
     2881 gcatcatccc caggacgttg cgccccttcg aaaggacgcc cgccacgttc cggctcgagc
     2941 cgttcgggtt cgccgactcg tcgacgtgcc cgtccccgtc gcagtatcgg aacacgaccc
     3001 ggtcctcccg ctcgagccgg tccagcgtct ccgggtcggc gtagtagttg ccgtcgccgt
     3061 gcgcgatcgg aacgcgcacc acgtccccct cctgcccgag ggtgaacggc gtggcgccgg
     3121 tctcgacccg caggtgcacg tgccggcaca cgaagcgcag cgacgcgttg cgcatcagcg
     3181 ctcccggcag gagcccgctc tcgcacagga tctggaatcc gttgcagatg cccagcacga
     3241 gaccgccgcc ttccgcgaac cggaccacct cgcgcatgat ggggctgaag cgggcgatgg
     3301 ccccggagcg caggtagtcg ccgtacgaga acccgcccgg cacgacgacg aggtccacgc
     3361 ctcccaggtc ctcctccttg tgccagatga agcgcgtctc gagccccagg acgtggcgcg
     3421 ccacgtggta cgcgtcgtga tcgcagttcg agccggggaa tacgagaacg ccgaccgatg
     3481 cggacatgcc ctgggacggt ggattcggga agcccgaaca tacgaaaggc gcggctccgg
     3541 atgaccggcg ccgcgccctt cgcgtgcgaa tcacggaagg gatcagtcct gcacggcctg
     3601 gatctcgagg aggatcctca ccttctccga gacgatgagg ccgcccagct cggtgaccgc
     3661 gtcccatttc aggccgtagt cgaagcggct gatctcggtc gaggcctcga tggcgacgcg
     3721 ttggctctcg cccatgctcg ccgaccccag gtacgtgccg tcgagcacga cctcgcgcgt
     3781 ggtgccgcgg atcgtcaggt ctccgacgag ctcgaactcg tcgccttcca cgttgcgcac
     3841 gcccttgctc acgaacgtga tcacgggatg gttcgccgca tcgaagaagt cgtccgactt
     3901 caggtggccg tcgcgcttct cgacgcccgt gtcgaccgag ccgacctgga tcgtggccgt
     3961 cgcttccagg gtagagagat cgtcgggatc catgcggacc gtggcgtcga aatcagcgaa
     4021 ccggccgttc acgttcgtaa tgccgaggtg gcggaccttg aacccgatgc tcgaatgggc
     4081 ctcgtccacc gaatacgtgg gcacgtcgac ggcggtcgtg tcggtgccgc cgtgaatgcc
     4141 gggagccgag aatgcgatga ctgcgggaag cgctgctgcg tgaaggatgt gggtcatggt
     4201 ggcttgctcc tgttgtgctg tcgttccgat gatttctgac ccggttacga agtccgggtc
     4261 attccgtgtt tcaacaatat acgttataac atagaaattg gttcccgact cggacaacat
     4321 ggctgcaggt cgtggcgtta tatgcgattc agcacgatcc ggcatgatcc caaaggaact
     4381 cttcgcaacg atccgccgac tggagatccg gaccaggggc ctggtc
//
LOCUS       DBNHQZ010000234         6435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_341096, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000234 DBNHQZ010000000
VERSION     DBNHQZ010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_341096"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="AC16YU_06225"
     CDS             <1..575
                     /locus_tag="AC16YU_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR6064854.1"
                     /translation="RAARERREGLRSELGESRRRAARKKPRRAAPPAPEPADAARPIA
                     VGDQVVVDGQGPAGDVLEVRESEAVVAVGSARMRVKLDRLARGGGARRQQVTIRRASA
                     DSGAWTPTQARTRIDLRGRRVDEALAEVVRLVDEAVAAGLPSVEILHGKGTGALRSAI
                     HEYLAGRDDVSGFEEAPVEQGGAGVTVVRI"
     gene            594..1556
                     /locus_tag="AC16YU_06230"
     CDS             594..1556
                     /locus_tag="AC16YU_06230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6064855.1"
                     /translation="MARIVITRPLPAEEVNPLEEAHDVRILDIGPDNGGDEDRMVEAV
                     ADADALITLLDNPVTARVLAASPNLRVVAQCAVGYDNIDLEAAAELQIAVTHTPGVLT
                     DATADQTMALMLAAARRLREADRYLRDGRFARWEMLELLGMGLAGKTLGIVGLGRIGS
                     AVARRALGFGMRIIYHDRKPINPTIERMAGAHRVDLDTLLAESDVVSLHCPLNPESHH
                     MIDAAALSRMKPLSILVNTARGPIVDEAALVDALRSGRIAAAGLDVFEDEPRIHPGLF
                     ELDNVVMTPHLGSATRLTRRAMARMCVEAVEAALAGEEIPYRIA"
     gene            1693..2100
                     /locus_tag="AC16YU_06235"
     CDS             1693..2100
                     /locus_tag="AC16YU_06235"
                     /inference="COORDINATES: protein motif:HMM:NF027349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HIR6064856.1"
                     /translation="MTRLLAAICLVLACPASPSHAQDTRPSELGAVARSIADGDASAV
                     LSLAGRRVEIGILGATRTYSRSQAEFVLERFFSEYPPEAVTLERATGTPDGWFASARY
                     RAARRTEPIRLYLRLRREGGEWRLREFVVREDT"
     gene            2143..6120
                     /locus_tag="AC16YU_06240"
     CDS             2143..6120
                     /locus_tag="AC16YU_06240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064857.1"
                     /translation="MRWSAAAIAVALIITAAWRMAVVMGATDVRARQQVLVERALDHA
                     ERDFRSIELAVLAEARARAASPDLVEALRRRRAGSPGIEPAVIAAAARLDVEALSSVE
                     FYDPTPRLLAWNGFSMPMDAAPGQPEFLSGEQAVLARDGDIRVALAAWAPVLDGDVAV
                     GAVRAMRLIELRMPVRNQYLRDYTLGEEWSRDTRMTMSVRYLGQGDTQRAAAPERRIL
                     RASDGTALVEVGAWPPTVDETVQMAVSRLDSLLALWASLLLLAAVWLAAARTSGARGA
                     AWRPLLAFTAVLWAARFALIALDVPNRWQTGKDPLSPLFDTAHLASSWGAGLAGTIGD
                     LAVTALFALTFALVVARRVFSRPAPEVTGQGRASIALRGLALGVAGSLAFWLASSLAE
                     RALLDSTLSYFARSGLLPPRLVVVVFSSLLMVLMAAWGLVAAAARVSVSGVVFNATAG
                     ERWTGLGSVLAGMAAVAWLAGMAAGTIGISLVLFMGIATYAGAATRVGDRLFVEFLSA
                     RGILFGIVLASLMLYPVLERGYDVERRDRMVDAADAFAEDRDPRILFAVESALDDGRT
                     TLAGVGEPAVLDSLMDYLLRGSLVASLGSFQVSAAVLEADGRPVARTGGRRSTSAAAA
                     LADQDRDDLELLRQIRAEQGGTGLVVEKLTSPHEFDRFDYVGAAPVSDDSGRWVLIRA
                     AQYEHIAAGSTPFPRVLVPAGYYGTRFPDLSIAEFRDDVLVRSFGVSFGRSYLEPAVV
                     ASLRTGASLWRRESLRDGRYVTYYTLKNPEEAVQDGPTVEMRTIAVRQAAVNVFDRLY
                     FLLRLTVSGLIVGLLAYSVGLLLRYRAGLLPAPRVRFRDRVLNAFFGVGLVTVAAMGA
                     LGLRVVTGENERAVQSWLRQHLERVEDTLMQEAGGDELPYRVLERMDIDTLAARVGLD
                     LNVYRGFELASTSRPQLARDRLIGMRLPVEAYQALFVDGYRFADVDERLGSFGYTAGY
                     RSFPDEQGVPRYVVSVPTLPEQDRLEEERARTVAYLFGALLLLVLVVMGTAAVIANAL
                     ALPVARIRRGLERVAEGRFDRIQPLDTRDEMSELVGTFNTMQDQLAESRRLLAQQERQ
                     LAWREMARQVAHEIKNPLTPMKLSVQIMQMAFTDPKEGRPFDEVFREKTAALIDQINA
                     LARIANEFSSYARMPLQSLEPVDLNAVVREASELMSANASIRFEMDLHEGPLTVEADR
                     EALRRVFINFVKNAVEAVPEDREGRIRITTDLEDDMETGLPWVVAEVSDNGCGIAQGL
                     REKIFTPSFSTKTSGAGLGLAIARNTVEELRGRIGFDTEEGRGSTFWVRIPLFQASEN
                     ADGSV"
     gene            6218..>6435
                     /locus_tag="AC16YU_06245"
     CDS             6218..>6435
                     /locus_tag="AC16YU_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR6064858.1"
                     /translation="MRAIIVGAGEVGFDVARLLAMEEHDVVVIDKDAAALSNVRERLD
                     VMTVHGNGTSARVLREAGVDGAAILVAVT"
BASE COUNT          871 a         2185 c         2399 g          980 t
ORIGIN      
        1 cccgggcggc gcgcgagcgg cgcgagggac tccggagcga attgggagag tccaggcgcc
       61 gcgccgcgcg aaagaagccg cgccgtgccg cgccgcccgc ccctgagccg gcggacgccg
      121 cgcgcccgat cgcggtgggc gaccaggtgg tcgtcgacgg ccagggtccg gccggagacg
      181 tcctggaggt ccgcgagtcg gaggccgtgg tggccgtcgg gtccgcgagg atgcgggtga
      241 agctcgaccg tctcgcccgg ggggggggag cgcgcaggca gcaggtcacg atccgccgcg
      301 cgtcggccga ctccggcgcg tggacgccga cgcaggcgcg cacccggatc gacctcaggg
      361 gccggcgcgt ggacgaggcg ctcgccgagg tggtccgcct cgtcgacgag gcggtggccg
      421 cgggtctccc gagcgtggaa attctgcacg gaaagggtac cggagccctg cgctcggcga
      481 tccacgagta tcttgccggc cgggacgacg tgtccggttt cgaggaggca cccgtcgagc
      541 agggcggagc cggggtcacg gtcgtccgga tctgaataca gagcacgcat cccatggcac
      601 gcatcgtcat cacgcgaccg ctaccggccg aagaggtaaa tccgctcgag gaggcgcacg
      661 acgtccgtat cctggacatc gggccggaca acggcgggga cgaggatcgc atggtcgagg
      721 cggtcgccga cgcggacgcg ctgatcacgc tcctggacaa tcccgtcacg gcgcgcgtgc
      781 tggccgcgtc cccgaacctc cgcgtcgtgg cgcagtgcgc cgtggggtac gacaacatcg
      841 acctggaggc ggcggccgaa ctccagatag cggtcaccca cacgccgggc gtcctgaccg
      901 acgcgacggc cgaccagacg atggccctca tgctggccgc cgcgcggcgg ctcagggagg
      961 cggaccgata cctgcgcgac ggacggttcg cgcggtggga gatgctcgaa cttctcggaa
     1021 tggggcttgc gggcaagacc ctgggcatcg tggggctcgg ccggatcggc agcgcggtgg
     1081 cccgccgcgc gctcgggttc ggcatgcgga tcatctacca cgaccgcaag cccatcaatc
     1141 cgaccatcga gcgcatggcc ggcgcgcacc gcgtggacct ggacaccctc cttgcggaga
     1201 gcgacgtggt gtcgctgcac tgtccgctga acccggagag ccaccacatg atcgacgcgg
     1261 cggcgctgtc gcgcatgaag ccgctgtcga tcctggtgaa cacggcgagg ggccccatcg
     1321 tggacgaggc ggcgctcgtc gacgctctcc ggtcggggcg catcgcggcg gcgggcctgg
     1381 atgtgttcga ggacgagccg aggatccacc ccggcctttt cgagctggac aacgtggtca
     1441 tgacccctca tctcggaagc gccacgcggc tcacccggag ggcgatggcg cgcatgtgcg
     1501 ttgaggccgt ggaggcggcg ctcgccgggg aggagattcc ctaccggatc gcctgagccc
     1561 ggccgtcccc tcaccgcggc gatgtgtcca cgggggcata cggccggcgg cctcccgggc
     1621 gtctgacggg ccggaatcgg cgacggacca gtttttgcta ttttgccagg ggaacctctc
     1681 aggtccggag caatgacccg gctactcgcc gccatctgtc tcgtgcttgc gtgcccggct
     1741 tcgccgtcgc atgcccagga cacccgtcct tccgagctgg gcgcggtggc gcggtcgatt
     1801 gccgacggcg acgcatccgc cgtgctctcg ctcgcgggga ggagggtgga gatcggcatc
     1861 ctcggagcca cgcggacgta cagccgttcg caggccgagt tcgtgctgga gcggttcttc
     1921 tcggagtacc cgcccgaagc ggtgacgctc gagcgggcga cggggacgcc ggacgggtgg
     1981 ttcgcctccg cgaggtaccg ggccgcgcgc cggacggagc cgatccggct gtacctgcgg
     2041 ctacggcggg aaggaggtga gtggcgcctg cgtgagttcg tcgtgcggga ggacacctga
     2101 gtgaccgacg cggctgaacg gtcccggttc ggatcggcac gcatgcgctg gtcggccgcc
     2161 gcgatcgcgg tcgccctgat catcaccgcc gcctggcgga tggccgtcgt gatgggtgcg
     2221 accgacgtgc gggcacgcca gcaggtgctg gtcgaacgcg ctctggacca cgccgagcgc
     2281 gatttccggt cgatcgaact ggccgtgctg gcggaggcgc gcgcgcgcgc ggcctcgccc
     2341 gacctggtcg aggccctgcg acggcgccgg gccggatcgc cgggcatcga gcccgcggtc
     2401 atcgccgcgg ccgcccgcct ggacgtcgag gcgctctcct ccgtggagtt ctacgatccg
     2461 accccccgcc ttctcgcctg gaacggtttt tcgatgccga tggatgccgc gcccgggcag
     2521 ccggagttcc tgtccggtga acaggccgtc ctggctcgcg acggggacat ccgggtcgcc
     2581 ctggcggcgt gggcgccggt cctcgacggc gatgtcgcgg taggggccgt ccgggcgatg
     2641 cggctcattg agctccggat gcccgtccgc aaccagtacc tgcgggacta tacgctcggg
     2701 gaggagtgga gccgcgacac ccgcatgacc atgtcggtcc gctacctcgg gcagggcgac
     2761 acgcagcgcg cggccgcgcc cgagcgccgg atcctgcgcg catcggacgg gacggccctc
     2821 gtcgaggtcg gggcctggcc gccgaccgtc gacgagaccg tgcagatggc cgtctcgcgt
     2881 ctcgattcgc tcctggccct ctgggcatcg ctgctgctgc tggcagccgt ctggctcgcc
     2941 gcggcccgga cgtccggcgc ccgcggagcc gcgtggagac cgcttctcgc attcaccgcc
     3001 gtcctgtggg ccgcgcgctt cgccctgatc gcgctcgacg tgccgaaccg gtggcagacc
     3061 ggcaaggacc cgctctcgcc gcttttcgac acggcccacc tggcgtcttc ctggggcgcg
     3121 ggcctcgcgg gcacgatcgg ggatctcgcc gtgaccgccc tcttcgcgct cacattcgcg
     3181 ctcgtcgtcg cccggcgcgt gttctcgcga ccggcgccgg aggtgaccgg acaggggagg
     3241 gcgtcgatag ccctccgggg cctggcgctc ggcgtcgcgg gatcgctcgc gttctggcta
     3301 gcgtcgtcgc tcgccgagcg tgcgctgctg gactcgacgc tgagctactt cgcccggtcg
     3361 gggcttctgc ccccgagact ggtcgttgtg gtgttttcga gcctgctcat ggtgctcatg
     3421 gcggcctggg gtctggtcgc cgcagcggcg cgcgtctcgg tgtcgggagt ggtcttcaac
     3481 gcgaccgcgg gcgagcggtg gaccgggctc gggtcggtgc tcgcgggcat ggcggcggtc
     3541 gcgtggctgg cggggatggc ggcggggacg atcggcatct cgctcgtcct cttcatggga
     3601 atcgccacgt acgccggcgc cgcgacccgc gtgggcgacc ggctgttcgt cgagttcctg
     3661 tcggcgcgcg gcatcctgtt cggcatcgtc ctcgcctcgc tcatgctgta cccggtcctg
     3721 gaacggggtt acgacgtcga gcggcgggat cgcatggtgg acgcggccga cgcctttgcg
     3781 gaggaccgcg atccacggat cctcttcgcc gtggaatcgg ccctggacga cggccggacg
     3841 acgctggccg gcgtcggcga gcccgccgtc ctggactcgc tgatggacta cctgctgcgc
     3901 gggtcgctgg tcgcctctct cggctcgttc caggtgtcgg ccgcggtgct cgaagccgac
     3961 ggacggccgg tggcccgcac cggcgggcgc cgatcgacgt ccgccgccgc ggccctcgcc
     4021 gaccaggacc gggacgacct ggaactgctc cgccagatcc gggccgaaca gggggggacg
     4081 ggcttggtcg tggagaaact cacgagcccg cacgagttcg accggttcga ctacgtgggg
     4141 gccgcacccg tctcggacga ctccgggcga tgggtcctga tccgcgcggc acagtacgag
     4201 cacatcgcgg ccgggagtac cccgttcccc cgcgtcctgg tccccgcggg ctactacggg
     4261 acgcgctttc ccgacctctc catcgcggag ttccgggacg acgtcctggt gcgttcgttc
     4321 ggggtgtcgt tcggccgctc ctacctggaa cccgccgtcg tcgcgtcgct ccggaccggc
     4381 gccagcctct ggcggcgcga atcgctgcgc gacggacggt acgtgacata ctacacgctc
     4441 aagaacccgg aagaagcggt ccaggacggg cccacagtgg agatgcgcac gatcgccgtg
     4501 cggcaggccg cggtgaacgt gttcgaccgg ctgtatttcc tcctgcggct cacggtatcc
     4561 ggactgatcg tcggccttct ggcgtactcg gtcggtctcc tgctgcggta ccgcgcgggg
     4621 ctgctgcccg ccccccgcgt ccggttcagg gaccgggtgc tgaacgcttt cttcggcgtc
     4681 ggcctggtca ccgtggcggc gatgggcgct ctcgggctcc gcgtggtcac gggcgagaac
     4741 gagcgggccg tccagagctg gctgcggcag cacctggagc gcgtggaaga caccctgatg
     4801 caggaggcgg ggggagacga gcttccctac cgcgtgctcg agcggatgga catcgacacg
     4861 ctcgcggcga gggtggggct ggacctgaac gtgtaccggg gattcgaact cgcgtcgacc
     4921 agccggccgc agctcgcccg ggaccgcctg atcggcatgc gcctgccggt cgaggcctac
     4981 caggccctct tcgtggacgg ttaccgcttc gcggacgtgg acgagcggct gggctcgttc
     5041 gggtacacgg ccggctaccg ctccttcccc gacgagcagg gcgtgccccg gtacgtggtg
     5101 tcggtgccga cgcttcccga gcaggaccgg ctcgaggagg agcgcgcccg cacggtggcg
     5161 tacctgttcg gagcgctcct gctcctcgtg ctcgtggtca tgggcacggc ggccgtcatc
     5221 gcgaacgcgc tcgcgctccc ggtcgcccgc atccggcggg gtctggagcg ggtggcggaa
     5281 gggcgtttcg accggatcca gccgctcgac acgcgggacg agatgagcga gctcgtgggc
     5341 acgttcaaca cgatgcagga ccagctggcc gagagccgtc gcctgctcgc gcagcaggag
     5401 cgacagctgg cctggcgcga gatggcccgg caggtcgccc acgagatcaa gaatccgctc
     5461 acgcccatga agctgtcggt gcagatcatg cagatggcgt tcacggatcc gaaggagggg
     5521 agaccgttcg acgaggtgtt ccgcgagaag acggcggccc tcatcgacca gatcaacgcc
     5581 ctggcgcgca tcgcgaacga gttctcgtcc tacgcccgca tgccgctcca gtcgctggag
     5641 cccgtggacc tgaacgcggt cgtccgcgag gcctcggaac tcatgagcgc caacgcgtcg
     5701 atccgattcg agatggatct gcacgagggg cccctcaccg tggaggccga ccgggaggcc
     5761 ctgcggcgcg tgttcatcaa cttcgtgaag aatgccgtcg aggccgtgcc cgaggaccgg
     5821 gaagggcgga tccgcatcac gacggacctc gaggacgaca tggagaccgg cctgccgtgg
     5881 gtggtcgccg aggtgagcga caacggatgc ggcatcgccc aggggctgcg cgagaagatc
     5941 ttcacaccgt cgttctctac caagaccagc ggggcgggcc tcgggctcgc gatcgcgcgg
     6001 aacacggtcg aggagcttcg cgggcgcatc gggttcgaca ccgaggaggg ccgggggtcc
     6061 acgttctggg tccggattcc gctgtttcag gcgtcggaga acgcggacgg gtccgtttga
     6121 cgtgcccgcg ggtcgggcta tcttgggccg cgtcggaaac cagtcagcat gcccgtcgga
     6181 gcccgccagg ggccgataac aggtgggaac gagccgtatg cgagccatca tcgtcggtgc
     6241 gggagaagtg ggcttcgacg tggcccgtct gctcgcgatg gaggagcacg acgtcgtggt
     6301 gatcgacaag gacgcggcgg cgctctcgaa cgtccgcgag cggctcgatg tcatgaccgt
     6361 gcacggcaac ggcacgtcgg cgcgcgtgct ccgcgaggcg ggggtcgacg gggccgccat
     6421 cctggtggcc gtcac
//
LOCUS       DBNHQZ010000235         4800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2235989, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000235 DBNHQZ010000000
VERSION     DBNHQZ010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2235989"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="pstA"
                     /locus_tag="AC16YU_06250"
     CDS             complement(<1..657)
                     /gene="pstA"
                     /locus_tag="AC16YU_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6064859.1"
                     /translation="MFDATSLNVRNDRVQQRFRLLFLLMTILLIVPVAVILGLLIHHG
                     APVISWTFLFEFPEQGMTAGGIFPALLGTIFLVSLALVFSVPLGVAAAIYLSEYAPDN
                     WLTRAINLAIINLAGVPSIVHALFGVGAFVIFFEFGTSILAASLTLAIMALPVVIVAT
                     RESLQAVPRAFREACWALGATRWQTIRKVVLPNSISGILTGVILEVSRTAGETAPIMF
                     T"
     gene            complement(661..1587)
                     /gene="pstC"
                     /locus_tag="AC16YU_06255"
     CDS             complement(661..1587)
                     /gene="pstC"
                     /locus_tag="AC16YU_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR6064860.1"
                     /translation="MSAEAVGAGRRTMDRIDRAWVVDKAAQVLMFIGGISAIIFILGI
                     FAFITREGLSFVFSRFSVAEFFGSIRWAPTSIANPTFGALALMAGTAAVTGMAMLVAI
                     PFSLGAAIYIAEFATGRTREILKVAVELLAAIPSVVWGFIGLSLMNAFIIEFFDVPIG
                     LNVLNAGIILGLMAAPIMTTIAEDALKAVPDRYREAAEAMGATRWQIIRRVVLPAARN
                     GLASAVLLGVGRGFGETMAVLMASGHSINIPSSVFDSVRALTATIAAELGETAVGSDH
                     YGALFTLGLFLFVVTFAINLTADLVVRGIRKK"
     gene            complement(1584..2660)
                     /gene="phoU"
                     /locus_tag="AC16YU_06260"
     CDS             complement(1584..2660)
                     /gene="phoU"
                     /locus_tag="AC16YU_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR6064861.1"
                     /translation="MTQYEQRLQTDLDALRQSVGTLATHVESNLRNAVRALLALDGDL
                     AYRTILKDHPVNRMAEDVEAQAHRFIARHLPSAGHLRFVSSVMRIVVLLERMGDYAVT
                     IGRETVQLERPLEGTFRDEVQAMAEDSLQMFSQAMVAFRERDVSLARGTMGFADQVDR
                     DFSVAFNMLVGEMPATLRQTDLFGRLIVIMQLERVSDQAKNLCEETIFALTGETKTRR
                     PVRVLFLDRTDASDTQMAVAIGRKRYGDRGVFRSAGSTPARAVDPDVAAFMEAHGFDM
                     SAARPSAIDWPDEDWAGFDVVVTLEGSIRDYLGTVPFHTVALAWEPAGARGTDLPDRY
                     RELSQAIGDLIDTMRAPAGDADAK"
     gene            complement(2743..3627)
                     /locus_tag="AC16YU_06265"
     CDS             complement(2743..3627)
                     /locus_tag="AC16YU_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6064862.1"
                     /translation="MKRTLVALSLLLFAAACGRPEGGSSRAVIENKGSDTLVNVAQAW
                     AEAYRTVNPDVAVAVTGGGSGTGIAALINGTVDIANSSREIKDEERAMAQANNHTPVE
                     HIVGYDALAVYVHPDNPLAEVTLEQLAGIYGETGTIETWSQLGISVPGCSSDEVVRVS
                     RQNNSGTYEYFREAVLGEGDFKMGSRDMHGSKDVVDLVQNTPCAIGYSGLAYATESVK
                     MPCVVDSAGTCVAPSEETAIDGSYPIARPLFMYTDGAPTGPVREYMDWILSDEGQCII
                     AEKGYAPYRPVTCPGGAG"
     gene            complement(3663..4646)
                     /locus_tag="AC16YU_06270"
     CDS             complement(3663..4646)
                     /locus_tag="AC16YU_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6064863.1"
                     /translation="MFLRTSIASILLLGATATVSSAQTGPKLGGLAYIDYYYVLDDPA
                     GVGDGDNGFSYRRLYLTGDHSMSENVRFRARLEANDASTNEDGLPTPFVKDLYVRWAN
                     AVGDGHEVLIGITSPPAFDTSEGFFGYRSLEKTQMDLRGIVSSRDFGVRVQGPVVATG
                     RIRYAVMLGNNSSVKRETDRHKRLYAQLEFQPVDRLTVTLGGDYAAMDDGSAVTANGF
                     AGYRFDRFRIGAEAFLSPVRRDAPLEDADRSGMSVFAAAQASPSVELLLRYDRYSAGM
                     DGIDETGEFALAGAAFTVENRLRLIPNLVVNRAPGADDPMLIGRITVEANF"
BASE COUNT          820 a         1558 c         1665 g          757 t
ORIGIN      
        1 cgtgaacatg atgggcgccg tctcgccggc cgtgcgggac acctccagga tcacgcccgt
       61 caggatgccg ctgatcgagt tagggagcac caccttccgg atcgtctgcc accgggtcgc
      121 gccgagcgcc cagcaggctt cccggaacgc gcgcgggacc gcctgtagcg attcgcgggt
      181 ggcgacgatc acgacgggca gcgccatgat cgccagcgtg aggctcgcgg ccaggatcga
      241 ggtgccgaac tcgaagaaga tgacgaaggc cccgaccccg aacagtgcgt gcacgatgga
      301 cggcacgccg gccaggttga tgatcgcgag gttgatcgcg cgcgtgagcc agttgtccgg
      361 ggcgtactcg ctcaggtaga tggccgcggc caccccgagc ggcaccgaga agacgagggc
      421 gagcgagacc aggaagatgg tcccgagcag cgccggaaag atgccccccg ccgtcatgcc
      481 ctgttccgga aactcgaaga ggaaggtcca ggaaatgacc ggggcgccgt gatggatcag
      541 aagtcccagg atgacggcga ccggcacgat gagcaggatc gtcatcagga ggaacagcag
      601 gcggaagcgc tgctgcaccc ggtcgtttcg gacgttcagg gaggtggcgt cgaacatggc
      661 ctacttcttc cggattccgc ggaccacgag atcggccgtg aggttgatcg cgaatgtgac
      721 cacgaagagg aaaaggccca gcgtgaagag cgcgccgtag tggtccgatc cgacggccgt
      781 ctcgccgagc tccgccgcga tcgtggccgt gagggcccgc accgagtcga agacgctcga
      841 cgggatgttg atcgagtggc cgctcgccat caggaccgcc atcgtctcgc cgaagcccct
      901 gccgacgccc aggagcacgg ccgacgcaag gccgttccgg gccgcgggga gcaccacccg
      961 gcggatgatc tgccagcggg tggcccccat cgcctccgcc gcctcccggt agcggtcggg
     1021 gacggccttg agcgcatcct ccgcgatggt cgtcatgatg ggcgccgcca tcagccccag
     1081 gatgatgccg gcattgagca cgttcagccc gatcggcacg tcgaagaact cgatgatgaa
     1141 ggcgttcatc agcgagagcc cgatgaagcc ccagacgacc gacgggatgg cggccaggag
     1201 ctccacggcc accttcagga tctcgcgggt acggcccgtg gcgaactccg cgatgtagat
     1261 cgcggcgccc agcgagaacg gaatggcgac aagcatcgcc atgccggtca ccgccgccgt
     1321 accggccatg agggccagcg cgccgaacgt cgggttggct atggacgtcg gcgcccagcg
     1381 gatggacccg aagaactcgg ccacggaaaa ccgggagaac acgaacgaca ggccttcccg
     1441 ggtgatgaag gcgaagatcc ccaggatgaa gatgatcgcg gagatgccac cgatgaacat
     1501 cagcacctgg gccgccttgt cgacgaccca cgcccggtcg atccggtcca tcgtcctccg
     1561 tcccgccccg acggcttcag cgctcatttc gcatccgcgt cgccggccgg ggcgcgcatg
     1621 gtgtcgatga ggtcgccgat cgcctgcgac agctcgcggt accggtccgg caggtcggta
     1681 ccccgtgcgc ccgcgggctc ccaggccagg gcgacagtgt ggaacggcac cgtcccgagg
     1741 tagtcccgga tcgagccctc gagtgtcacg acgacgtcga agcccgccca gtcctcgtcc
     1801 ggccagtcga tggcggaggg tcgggccgcg gacatgtcga atccgtgcgc ctccatgaag
     1861 gcggcgacgt cgggatccac ggcccgggcg ggggtggagc ccgcgctccg gaaaacgccc
     1921 cggtcgccgt accgcttccg gccgatcgcg acggccatct gcgtgtcgga cgcgtcggtc
     1981 cggtccagga agagcacgcg gacgggccgc ctcgtcttgg tttcgcctgt gagcgcgaag
     2041 atggtctcct cgcacaggtt cttggcctgg tcgctcacgc gctcgagctg catgatgacg
     2101 atcagccgcc cgaacaggtc cgtctgccga agcgtggccg gcatctcgcc aacgagcatg
     2161 ttgaacgcga ccgagaaatc gcggtccacc tggtccgcga agcccatcgt cccccgggcg
     2221 agcgacacgt cccgctcgcg gaaggccacc atcgcctggg agaacatctg gagcgaatcc
     2281 tcggccattg cctggacctc gtccctgaac gtgccctcga gcggccgttc cagctgcacg
     2341 gtttcccgcc cgatcgtgac cgcgtagtca cccatgcgct cgagcaggac gacgatccgc
     2401 atcacggacg aaacgaaccg caggtgaccg gcggacggaa ggtggcgtgc gatgaagcgg
     2461 tgggcctgcg cctcgacgtc ttccgccatg cggttcaccg gatggtcctt caggatcgtg
     2521 cggtatgcca ggtccccgtc gagggccagc agcgcccgca cggcgttgcg caggttggac
     2581 tcgacgtggg tggcgagcgt cccgacactc tgccgcaggg cgtcgaggtc ggtctgcaga
     2641 cgctgttcgt attgcgtcat gggatggctc gatcaggtgg aggccggacg gcccggaaag
     2701 gcgaccggcc cgcgatccac ggtgcgtgct cgatggggac ggtcagcccg cgccgcccgg
     2761 gcaggttacc ggacggtacg gcgcgtaacc cttctccgcg atgatgcact gaccctcgtc
     2821 gctcaggatc cagtccatgt actcccggac cggacccgtc ggggcgccgt ccgtgtacat
     2881 gaagagcgga cgcgcgatgg ggtaggagcc gtcgatcgcg gtctcttcgg agggcgccac
     2941 gcacgtcccg gccgaatcga ccacgcaagg cattttcacg ctctcggtcg catacgccag
     3001 gccggagtag ccgatcgcgc acggcgtgtt ctgcacgagg tcgacgacgt ccttggatcc
     3061 gtgcatgtcc cgggatccca tcttgaagtc cccctcgccg agcacggcct cacggaagta
     3121 ttcgtaggtg ccggagttgt tctgacggct gacgcggacg acctcgtcgc tcgagcagcc
     3181 gggaacgctg attccgagct gcgaccaggt ctcgatcgtt ccggtctcgc cgtagatgcc
     3241 ggcgagctgc tcgagcgtga cctcggcgag cggattgtcg gggtggacgt acacggcgag
     3301 cgcgtcgtag ccgacgatgt gctccaccgg cgtatggttg ttggcctggg ccatcgcgcg
     3361 ctcctcgtcc ttgatctcgc gggaggaatt cgcaatgtcg acggtgccgt tgatgagggc
     3421 ggcgattccg gtgccggatc cgccgccggt caccgcgacc gccacgtcgg gattgacggt
     3481 ccggtaggcc tcggcccagg cctgggcgac gttcacgagc gtgtccgagc ccttgttctc
     3541 gatgacggcg cgggaggatc cgccctcggg acggccacag gccgcggcga agagaaggag
     3601 ggagagagcg acgagcgtgc gtttcatgga tgacccgccg tgcgggttgt gtctggtatc
     3661 gatcagaagt tggcctcgac cgtgattcgg ccgatcagca tcgggtcgtc cgcgccggga
     3721 gcgcggttga cgacgaggtt cgggatgagg cggagccggt tctccacggt gaacgcggcg
     3781 ccggcgaggg cgaattcgcc cgtctcgtcg attccgtcca tgccggcgga gtagcggtcg
     3841 tagcggagca gcagctccac ggacggggat gcctgggccg cggcgaatac gctcattccg
     3901 gagcggtccg cgtcctcgag gggcgcgtcc cgtcgaacgg ggctcaaaaa ggcctccgcg
     3961 ccgattcgga accggtcgaa ccggtagccg gcgaagccgt tggccgtgac ggcggatccg
     4021 tcgtccatgg ccgcgtagtc tccgcccagc gtgacggtca gtcgatccac gggctgaaat
     4081 tcaagctggg cgtacaatct cttgtgcctg tcggtctcgc gcttcaccga actgttgttt
     4141 ccgagcatga cggcgtagcg gatacggccc gtggccacga cagggccctg gacccggacg
     4201 ccgaaatcgc gcgacgaaac gatcccgcgc aggtccatct gcgtcttctc cagcgagcgg
     4261 tagccgaaga atccttccga cgtgtcgaac gccggtggcg aggtgatccc gatgagcacc
     4321 tcgtggccgt cgccgaccgc gttcgcccac cggacgtaca ggtccttgac gaacggtgtc
     4381 ggcagcccat cctcgttcgt cgaagcgtcg ttggcctcca ggcgcgcccg gaaccggacg
     4441 ttttcggaca tcgagtggtc gcccgtcagg tacagacgac ggtaggagaa gccgttgtcg
     4501 ccgtccccga caccagccgg gtcgtccagg acgtagtagt agtcgatgta ggcgagtccg
     4561 ccgagcttcg ggccggtctg cgccgaggag acggtcgccg tcgcgcccag aagaaggatc
     4621 gaggcgatgg atgtgcggag aaacaacggc ttcaggtaca ttgtgtcttg gatgaccgag
     4681 tgtagaatcg aacggttacg aggcggttac cggaccatta tccgaatgtt accgaccaat
     4741 cttgacaccg tatcccgggt cgacgtctgg gcgctgatca tcggcgtcgt cggcgggctc
//
LOCUS       DBNHQZ010000236         4493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2101626, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000236 DBNHQZ010000000
VERSION     DBNHQZ010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2101626"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1260
                     /locus_tag="AC16YU_06275"
     CDS             196..1260
                     /locus_tag="AC16YU_06275"
                     /inference="COORDINATES: protein motif:HMM:NF017394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HIR6064864.1"
                     /translation="MLDTRSRIVLALAALLLVVMYFTPLWRIRLEAPQYPEGIGLKIM
                     LSTVAGQKPNDLANINNLNHYIGMKRIVPEAIPELRIMPWAVGLLIVLGLAAAASGRV
                     WLLYTWVGLFTVLAVAGLIDFWLWEYDYGHNLDMEKAIIKVPGMSYQPPLIGSKQLLN
                     FKAHSWPGIGGWAAFVSLAAGVALVARRLLSGRRRRLAVIRPMGLAAASLVAMLVVSG
                     CARGPEPFVFGEDQCAHCRMTITDPAYATQVVSTTGRAYKLDSAECLLRFLEAETVTV
                     DEIHSSWVSAVDTGELVRGESAWFVQSPSIRSPMGGGLAAFSTQDAAAAAAGGNDGRL
                     MDWEAFRSGAMTAATHRHHP"
     gene            1257..2588
                     /locus_tag="AC16YU_06280"
     CDS             1257..2588
                     /locus_tag="AC16YU_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HIR6064865.1"
                     /translation="MKAAALIAIAALTAWAGVTLGPGADAVPPAAGAPTVAASSDAPA
                     AFSIADAVRAAAPHDTVRVPAGTYREETVVIDRPLVLLGLGRPVLDGEGARGLLEVTA
                     DSVTVRGFVFRNTGITFMDDRAALRVSGSRGCVILDNAFEETFFGVYLARAEDCVVAG
                     NTFRTTADREANGGNGVHSWYSRRLLVEGNRVSGHRDGIYLEFTEDSRVVGNVSTDNT
                     RYGLHFMFSDRCSYERNRFERNGAGVAVMYADDVEMRGNDFLDTWGASAYGLLLKEIK
                     GGSVADNTFARNSVALFVEGTDRMTFDGNRLEDNGWAIKLMANATDNEFRGNGFRRNT
                     FDVATNSRSATSRFAGNYWDRYRGYDLDRDGTGDVPFHPVRLFSVIVERHEPALVLLR
                     SPLVDVLDAVERVLPVLTPDALVDTAPLMKPPAAPPEAAGTDAPVEAPPTA"
     gene            2585..3355
                     /locus_tag="AC16YU_06285"
     CDS             2585..3355
                     /locus_tag="AC16YU_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064866.1"
                     /translation="MIARVPDTDSAIDIRGLHKAFGSRPVLSGVDLSFERSGVTAVIG
                     PNGAGKTTLIKCLLGLVRPDEGSIAVGGDLVSGPAATGRRRIQGDVSYRERIGYMPQQ
                     AAFPDNLTGREVVALIRSVRRPDLPTDFELMRTLQLDAEMEKPVRTLSGGTRQKLSAL
                     LAFLFRPPILVLDEPTAGLDPVASAALKDHVRAAADGGATVLLTSHVMADLEELCDRV
                     VFLLDGRVRFDGTLEAIRESTGEERLERAIAHILTRAA"
     gene            3387..4178
                     /locus_tag="AC16YU_06290"
     CDS             3387..4178
                     /locus_tag="AC16YU_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6064867.1"
                     /translation="MNEISVIARSQIHDALRSRWLLLHGLLYLVITEGLILAGGLTDK
                     ALLSLVNVVLLLVPLVAIVFGAIHLYNARDYILLLLTQPVRRHSLFFGLYAGLAGPLA
                     GTFALGVAAPFAWHARITADNLPTLLAILGLGALLTLVFVAVALLVAIRTRDRVRGIG
                     TALAIWLGAAIAYDGLVLLAVASFAAYPIEKPLLVIMMLNPVDLARVVLLMLMDAGAL
                     MGYTGAVFGRFFGSGPGMLLAIGALFVWAAVPLVAARRAFLRSDF"
     gene            complement(4248..>4493)
                     /locus_tag="AC16YU_06295"
     CDS             complement(4248..>4493)
                     /locus_tag="AC16YU_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR6064868.1"
                     /translation="GGVRLALPADQISVRRVIEEIDGDDLFTECVLGLPGCGDEAPCP
                     MHGRWAAIRTAVAASFAQQSIGDLALGYDSGTMRLRP"
BASE COUNT          608 a         1648 c         1514 g          723 t
ORIGIN      
        1 tctatccctt ctactgcacg gacttctgct cggcgctcca ccaggagatg cagggctaca
       61 tccgggtctc accccgcggc agcgacgtgc cgctgaccca caacgccggt cgatgacggg
      121 ggcgacgccg acgggtcggg gcgcctcgcg cgccccggcc cggccggcgc cggattcccg
      181 caaccggaca gcaccatgct cgacacgcgc tcacggatcg tcctggccct tgccgccctg
      241 ctgctcgtcg tgatgtactt cacgccgctg tggcgcatcc ggctcgaggc gccccagtac
      301 cccgagggca tcgggctcaa gatcatgctc tcgaccgtcg ccgggcagaa gcccaacgac
      361 ctcgcgaaca tcaacaacct gaaccactac atcgggatga agcgcatcgt gcccgaagcg
      421 atccccgagc tccgcatcat gccctgggcc gtgggcctgc tcatcgtgct cggcctcgcg
      481 gccgccgcct cgggacgggt ctggctgctg tacacctggg tcggcctgtt caccgtgctg
      541 gccgttgccg ggctcatcga tttctggctc tgggagtatg actacgggca caacctggac
      601 atggagaagg ccatcatcaa ggtgccgggc atgtcgtacc agcctccgct catcgggtcg
      661 aaacagctcc tgaacttcaa ggcccattcg tggccgggca tcgggggatg ggccgcgttc
      721 gtgtcgcttg cggccggcgt cgccctggtc gcccgccgcc tgctctcggg gcgccgtcgc
      781 cgcctggccg ttatccggcc gatgggcctc gccgccgcct ccctcgtggc gatgctcgtc
      841 gtctcgggat gcgcgcgcgg ccccgagccc ttcgtcttcg gcgaagacca gtgcgcgcat
      901 tgccgcatga cgattacgga tcccgcctac gcgacgcagg tcgtctcgac caccggacgc
      961 gcctacaagc tggactcggc ggaatgcctg ctccgcttcc tcgaggccga aaccgtgacc
     1021 gtggacgaaa tccactccag ctgggtgtcg gccgtcgaca ccggggaact tgtgcgcggg
     1081 gagtcggcct ggttcgtaca gagtccgtcg atccgcagcc cgatgggggg tggcctcgcc
     1141 gccttctcca cgcaggacgc ggcggctgcc gcggccggcg ggaacgacgg caggctcatg
     1201 gactgggagg cgttccgctc cggagcgatg acggcggcga cgcaccggca tcacccgtga
     1261 aggcggctgc cctcatagcg atcgcagcgc tcaccgcgtg ggccggcgtg acgttggggc
     1321 cgggcgcgga tgcggtaccg cccgcggcgg gtgccccgac ggtcgccgcg tcgtccgacg
     1381 ccccggccgc cttctcgatt gccgacgccg tccgcgcggc cgcgccgcac gacacggtgc
     1441 gcgtgccggc cgggacgtac cgcgaagaga ccgtcgtcat cgaccggccg ctcgtgctcc
     1501 tcggccttgg ccgtccggtg ctggacgggg agggggcacg cggcctcctg gaggtcacgg
     1561 ccgactcggt gaccgtccgc ggcttcgtct tccggaacac gggcatcacg ttcatggacg
     1621 accgtgccgc cctgcgggtg agcggcagcc gcgggtgcgt catccttgac aacgcgttcg
     1681 aggagacgtt cttcggcgtc tacctggccc gcgcggagga ctgcgtggtg gccggcaaca
     1741 ccttccggac cacggcggat cgcgaggcga acggcgggaa cggcgtccac tcgtggtact
     1801 cccgccggct cctggtcgag ggcaaccggg tctcggggca ccgcgacggc atctacctcg
     1861 agttcaccga ggacagccgg gtcgtcggca acgtctcgac cgacaatacc cgctacggac
     1921 tgcacttcat gttctcggac cgctgctcct acgagcggaa ccgcttcgag cggaacgggg
     1981 cgggggtcgc cgtgatgtat gccgacgacg tcgagatgcg cggcaacgac ttcctggaca
     2041 cctggggcgc ctccgcgtac gggctcctgc tcaaggaaat caagggaggg agcgtcgccg
     2101 acaatacgtt cgcgcgcaac tcggtggccc tgttcgtcga ggggaccgac cgcatgacct
     2161 tcgacggcaa ccgtcttgag gacaacggct gggcgatcaa gctcatggcg aatgcgacgg
     2221 acaacgaatt ccgcggcaac ggcttccgcc gcaacacctt cgacgtcgcg acgaacagcc
     2281 ggtccgcgac gagccgcttt gccggcaact actgggaccg ctaccggggc tacgacctgg
     2341 accgcgacgg cacgggagac gtgccgttcc atcccgtgcg gctcttctcc gtcatcgtgg
     2401 agcgtcacga gccggccctg gtcctcttgc gcagcccgct ggtcgacgtc ctcgacgccg
     2461 tcgagcgcgt gctgcctgtc cttacgcccg acgcactggt cgacacggcc ccgctcatga
     2521 aacctcccgc ggcgcccccc gaggccgccg gcaccgacgc gcccgtggag gcgcccccca
     2581 ccgcatgatt gcccgcgtac ccgacacaga ttccgccatc gacatccggg ggctgcacaa
     2641 ggccttcggc tcgcggcccg tgctctcggg cgtcgacctc tcgttcgaac gctccggcgt
     2701 caccgccgtc atcgggccca acggcgccgg gaagaccacc ctgatcaagt gcctgctcgg
     2761 cctggtccgc ccggacgagg gctcgatcgc ggtcggcggg gacctggtct cggggccggc
     2821 cgcgaccggt cggcggcgca tccagggcga cgtttcgtac cgcgagcgga tcggctacat
     2881 gccccagcag gcggcgtttc cggacaacct cacggggcgc gaggtggtcg cgctcatccg
     2941 atcggtgcgt cggcccgacc tgccgacgga cttcgagctg atgcgcacgt tgcagctcga
     3001 cgccgagatg gagaagcccg tgcgcaccct ctcgggcggc acacgccaga agctgagcgc
     3061 gctcctcgcg ttcctcttcc gcccgccgat cctggtcctc gacgagccga ccgccggcct
     3121 ggatcccgtc gcgagcgcag cgctcaagga ccacgtccgt gcggccgcag acggcggagc
     3181 caccgtgctc ctgacgagcc acgtcatggc cgacctcgag gagctgtgcg accgcgtggt
     3241 cttcctgctc gacggtcgcg tccggttcga cgggacgctc gaagccatcc gcgagtccac
     3301 gggagaggag cgtctcgagc gcgccatcgc ccacatcctg acccgggccg cgtgaccgcc
     3361 cccgccgtcc gtgcccccgc cctgccatga acgaaatctc cgtcatcgcc cggtcgcaga
     3421 tccacgacgc tctccggagc cgctggctgc tgcttcacgg cctgctgtac ctcgtcatca
     3481 ccgagggcct gattctcgcc ggcggcctga ccgacaaggc cctgctgagc ctcgtgaacg
     3541 tggtgctgct gctcgtgccg ctcgtcgcga tcgtcttcgg ggccatccac ctctacaatg
     3601 cgcgtgacta catcctgctg ctcttgacgc agcccgtacg gcggcattcg ctcttcttcg
     3661 ggctatacgc cgggctcgcc gggccgctgg cgggcacgtt cgcgctcggc gtcgccgcgc
     3721 cgttcgcatg gcacgcccga atcaccgcgg acaacctgcc cacgctgctc gcgatcctgg
     3781 ggctcggcgc gctgctgacc cttgtcttcg tcgccgtcgc gctcctcgtc gcgatccgca
     3841 cgcgcgaccg ggtgcgcggc atcgggacgg cgctcgcgat ctggctcggc gcggccatcg
     3901 cctacgacgg cctggtgctg ctcgccgtgg cgtccttcgc ggcgtacccg atcgagaagc
     3961 cgctgctggt gatcatgatg ctgaacccgg tggacctcgc ccgcgtcgtc cttctcatgc
     4021 tgatggacgc cggcgcactc atggggtaca ccggcgccgt cttcggacgg ttcttcggat
     4081 cgggccccgg aatgctcctg gcgattggcg ccctgttcgt gtgggccgcg gtgccgctgg
     4141 tcgcggcgcg gcgggcgttc ctccggagcg atttctgacg gcacctgccg cccgggcggg
     4201 cgggcggttt ccggtgatgg acgcgcgggc ggtgccggcc cgcagggtca cggcctgagc
     4261 cgcatcgtcc cggagtcgta ccccagtgcc aggtcgccga tcgactgctg cgcgaagctc
     4321 gcggcgaccg cggtccggat ggcggcccat cggccgtgca tggggcaggg ggcctcgtcg
     4381 ccgcatccgg ggagaccgag cacgcactcg gtgaagaggt cgtcgccgtc gatttcctcg
     4441 atgactcggc gcacgctgat ctgatcggcc ggaagggcga gccggacccc tcc
//
LOCUS       DBNHQZ010000237        12193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2373118, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000237 DBNHQZ010000000
VERSION     DBNHQZ010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2373118"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16YU_06300"
     CDS             complement(<1..328)
                     /locus_tag="AC16YU_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064869.1"
                     /translation="MLQEQSRLFQRLLFFADFVLVAVAWISAYFVRFEVLRAINFFPP
                     PEWVPFERYLSLLPWVLLLSMFVFWASGLYVPDRAQRWTSLVSGVVKAIIVGMVVTAA
                     ALSFYRA"
     gene            complement(442..1341)
                     /gene="nadC"
                     /locus_tag="AC16YU_06305"
     CDS             complement(442..1341)
                     /gene="nadC"
                     /locus_tag="AC16YU_06305"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR6064870.1"
                     /translation="MLPPYLTDVELDDFVRRALAEDIGSGDVTTRATIPPEALAEAEF
                     LAKSPGIVAGIEVVNRVFRALDGGVSLTWTLDDGARVAAGRRFGTVAGPARALLSGER
                     VALNLLQRMSGIATATRAMVDAIDDRPTRLLDTRKTVPGLRALDKWAVLLGGGHNHRI
                     GLYDQILVKDNHIAACGGIPEAIRAANAFRAAAGRRDLVVEIEVRTLEELDQVLDCGA
                     IDVILLDNMVSGAPDGTVDTSLLAEAVRRVGGRFRTEASGNVSIATVGAIAATGVQAV
                     SSGALTHSVAALDISLEMSLAVR"
     gene            complement(1347..1766)
                     /locus_tag="AC16YU_06310"
     CDS             complement(1347..1766)
                     /locus_tag="AC16YU_06310"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6064871.1"
                     /translation="MPRHVHPHRVRYREVDPMGLVYHVHCLDWFEAARTEALRSSGIS
                     YREIEEGGVSMPVVDLAVRYHRPVRYDDAIEIGVTWTGEAPVARIRFDYDVRREGEPD
                     VLVSGHVTLCFLDLATGRPVRAPRMLRDALERLALPS"
     gene            complement(1777..2772)
                     /locus_tag="AC16YU_06315"
     CDS             complement(1777..2772)
                     /locus_tag="AC16YU_06315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR6064872.1"
                     /translation="MAKPSTPQPLDFERPLIELEARLEEMRGLQSDESGPDLQANIRS
                     LEERVEELRGAIYRNLTRWQRVQIARHPMRPYSLDHIHAWTTGFEELHGDRLFGDDPA
                     MVGGLATFQGSRWGYRDRTVMVIGQQKGRDTKMRKYRRFGMPNPEGYRKALRLMRMAA
                     RFGKPVVTLLDTPGAYPGMEAEERGQAEAIARNLVEMSALPVPVVVVIIGEGASGGAL
                     GIGVGDRILMMENSWYSVISPESCSSILWRSWDYKEEAARALKLTATDLVPLGIIDEI
                     IAEPVGGAHRDPDAAYEAVGASIAANLKALDGIAPGDLRQARLEKFDAMGRYETE"
     gene            complement(2797..4857)
                     /locus_tag="AC16YU_06320"
     CDS             complement(2797..4857)
                     /locus_tag="AC16YU_06320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6064873.1"
                     /translation="MPTERADIPSPTPATVDAPDLSVVIVNYNVREFLAQALRSVERA
                     ARHLRVEVIVVDNNSVDGSVPMVRSRFPRVRLVPNDRNAGFGAANNQALELARGRYVL
                     ILNPDTIVQEDTLDTLVRFMDEHPGAGAVGCQILNPDGTFAPESRRAFPTPRVAFFRM
                     SGLGRLFPQSPVFGRYNMTFLSRDEEAEVDALSGSCMLVRRAALFGDPADPGRRGAGL
                     FDEAFFMYGEDLDLCFRIQQAGWSIHYTPATRIIHYKGESTKKGEFRYVRLFYGAMVR
                     FARKHLHGRYSGIFIAMLQAAIVVRATISLLTGAARRMAGPLADFATMYVVVAAAGWI
                     RSLAPDTAVHALFLVTVAPAFAAGTVLGIALTGGYRRSWEHRVRPVLAGAAIGFLLVA
                     ATSFFVPAIAFSRAVVAVSLPVGALLLSLRRLARQARASEPRLALLVGPMDEAVRLDG
                     MLRAHPRPPFRLIGYVDPDEPAEAPGGDGEAGSRSLGALHHLRDLVRLHRVDNVVFAT
                     RDLSNQHIFEVIQSLRDLPVEFRMLAEGRSHVIGKASVSELAVPELTASVSDAVRVRT
                     TAARRAFEIPAAVGGLLLVPFVLPAALAGGRDGRAARALRRLVQLPSVLSGRLSLVGV
                     HPDHAGLVPPDWRLKEGVFNIADVLEGRNPATDELGRAYGYYVTIQSAALDWEIIIRT
                     LTAR"
     gene            4976..6352
                     /locus_tag="AC16YU_06325"
     CDS             4976..6352
                     /locus_tag="AC16YU_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6064874.1"
                     /translation="MSRDSAYRTEVRTLLLLAGPVVLTQLAQVSLGFVDTVMVGRLGA
                     AQLAGVALGSTVFFTLIIMCMGMVLAVGPMVSQAYGAGTDEPIGRSVRQGQWLALMLA
                     AGFTLVVWNVRPVLLALGQDPSTVDLAVSYLRAIVWGAFPFLGFVGLRSFVEGVSRPR
                     PVTVIAFVGVGTNVFANWTLMYGKLGFPALGIVGTGWASAIVFWLDFLLLLAYVASQS
                     EFRRFRIFEKLGRPDRTYFRELFRIGWPIGASFGIESSMFMITVMMMGWIGTTALAAH
                     QVALQCASFTFMVPLGIGIATSVRVGQEVGRGDREGVLRAGRVGLGLSVLFMSLAALS
                     FWLVPRAIVGLYLDLDAPANAPVVALAVALLQVAAVFQVADGIQVTAAGALRGLKDTR
                     VPMAISAVSYWAVGLTGAWFLAFRLGWGATGLWWGLVLGLAAAAAMLLARFRSMATSG
                     PAIAPAFD"
     gene            6447..7094
                     /locus_tag="AC16YU_06330"
     CDS             6447..7094
                     /locus_tag="AC16YU_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6064875.1"
                     /translation="MAQIFPKRANRLPAVSIVGLLGGGIFVIFFVWYYFSPEYTDVGY
                     APEQPVAYSHRLHAGQLGVKCQYCHNWVETAPHANVPSTQTCMNCHGQIRTESLKLLP
                     VRESWATGTSIPWVKVHQLPEYVHFAHNVHVTNGVGCETCHGRVDQMDVVSLVEPLSM
                     GWCLECHRQPELYLRPDDRVTTMGYVQPADFVQRNLERVRTENIQPPTNCSACHY"
     gene            7164..10292
                     /locus_tag="AC16YU_06335"
     CDS             7164..10292
                     /locus_tag="AC16YU_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HIR6064876.1"
                     /translation="MIELPVLPTMPSADGAPRSRFWRSVQQLEGDPAYRAFTGSELLP
                     DASGPPSETSRRQFLQVMGASMALAGLTACRKPVEAIMPFARKPEDLVPGVPMHYATG
                     MEFRGALRPLVVESTDGRPTKVEGNPEHPESGGSTGVFEQASLLALYDPDRSRAVLRD
                     GNDSHWNAFVRFAAERHAGSASGRLAVITRPSSSMTRAAIRREMEAAWPGLRWVEYQA
                     VPDAAARAYGLGLGTAVHPRFQFERALVVLSLDGDFLGATDIDHVHNTRGFARSRAVD
                     QTGEMSRLYVAESAYTLTGGAADHRLAVRRSDLPALAAAIARRLGVEAGRGGSAWDSE
                     PWVAAVADDLRAAGPRALVVAGDDDEVQLLAVAMNRVLGAIGGTVEVLDTGMPAGGGA
                     DALDALVADMAAGRIDTLLALDANPVYDLPAALGFAQALGQVAETVHAGLYVDETARA
                     CRWHLPLAHYLEAWSDGRSAGGTRSVVQPLIAPLYEAARSDIEILGVLASGLDRDGYE
                     LVRSTWQPLLGGGFDAAWRRVVHDGFQPDSAYGTVQPSEAVEAAVASAGADGAFDAGL
                     QDGIEIVFHADSKVFDGSFANNAWLQELPDSISKVVWDNVALVSPSTAASLGLRVDLV
                     NGKHRVDRVSISTPDGSVDLPVWIAPGLARSSVHVSLGYGRNITSSRPTRPGHVFDLD
                     HRTDLYGQGAISSGVGVSVAPIRPGNLGMVVREGVTVTRSGGDYLVASTQDHGALPEE
                     GAEAMNRGLYRIATLEEYRANPDFVADSEPKPIREDWGDYPSLWQNAHPADQPASKDN
                     PYSANQWGMVIDLNACTGCNACLVACQAENNIQVVGQDQVSRGREMHWIRLDRYFVGD
                     GADGSPRMAVQPVPCMHCENAPCESVCPVAATVHSPDGTNQMIYNRCIGTRYCANNCP
                     YKVRRFNYYNWTKTLPDSLHMAQNPNVTVRSRGVMEKCSYCIQRVREANRRSSVENRP
                     IRDGEVVTACQQACPAGAIVFGDLADPESRVSSARKNPRRYELLAELSVKPRTSYLGR
                     IRNPNPRLEREA"
     gene            10338..11789
                     /gene="nrfD"
                     /locus_tag="AC16YU_06340"
     CDS             10338..11789
                     /gene="nrfD"
                     /locus_tag="AC16YU_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6064877.1"
                     /translation="MSTVPHSAQAAHAIDDPPLFGSDLSFHDVTELVSYHTEKKTPTA
                     WFGAFALANAGLLLLVLMIAYLFWNGSGVWGLNNPVGWGYAIVNFVFWVGIGHAGTLI
                     SAILFLFRQPWRTGINRSAEAMTIFAVICALVFPTIHVGRVWVIYWTLPVPNQMEMWP
                     QFKSPLLWDVFAVSVYFTVSLMFWYVGLVPDLASLRDRARTLWRRRVLGFLSLGWTGA
                     NRHWRNYEKAYLLLAALATPLVLSVHSVVSFDFAVSIVPGWHTTIFPPYFVAGAIFSG
                     FAMVVTLLVISRKVYGLENIITVNHLEKMNIIILLTGTMVGFAYITEFFVAWYSGVKY
                     EQYAFLNRAFGPYAWAYWIMMSCNLLFPQLFWFRKLRRSIPFMFAVSITTNIGMWFER
                     FVITVTSLHRDYLPSSWDYYSPTAVDILTFVGTFGLFFTLFLLFLRFVPMVAMAEVKY
                     VMPQADPHFYEGHGDGRAGEDEPAVVPSIPPTS"
     gene            11809..>12193
                     /locus_tag="AC16YU_06345"
     CDS             11809..>12193
                     /locus_tag="AC16YU_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR6064878.1"
                     /translation="MSTLKSVIGDVRASMGIFDGRDAGLYGLLAEFRNPGALYEAAGA
                     VRKAGYRRFDVHSPFPIHGMDRAMGLGNSRVGWFTLLGGASGLALGVWLQWWTGSVDY
                     PLNISGKPFFAVEPSIPIIFELTILF"
BASE COUNT         1802 a         4218 c         4247 g         1926 t
ORIGIN      
        1 acgcccggta gaacgatagg gcggccgccg tcaccaccat gcccacgatg atggccttca
       61 ccacgcccga aacgaggctg gtccagcgct gggcacgatc gggcacgtac agcccggagg
      121 cccagaacac gaacatggac aggagcagga cccagggcag caggctcagg tagcgctcga
      181 acggcaccca ctccggcgga gggaagaaat tgatcgcccg caggacctcg aagcgcacga
      241 agtacgccga gatccaggcc acggcgacca gcacgaaatc ggcgaagaac agcaggcgct
      301 ggaagagccg gctctgctcc tgcagcatgg gtcggtgcgg atcgtgatga acgtcgtaga
      361 ggtcgtggcg gcggcggtcc cgggtggatc gacacaagat acgccatccc cggggcccgg
      421 atcgggcgga atgggcgccg gtcagcgcac ggcgagcgac atttcgagcg agatgtcgag
      481 tgcggcgacc gagtgcgtca gtgcgccgga ggatacggcc tggacgcccg tcgcggcgat
      541 cgcccccacg gtcgcgatcg agacgtttcc ggaggcctcg gtccggaagc ggccgccgac
      601 gcggcggacg gcctcggcca gcaggctcgt gtcgacggtc ccgtccggcg cccccgatac
      661 catgttgtcc aggaggatca cgtcgatcgc gccgcagtcg aggacctggt cgagctcctc
      721 cagggtcctg acctcgatct cgacgacgag gtcacgccgg ccggcagcgg cccggaacgc
      781 gttggcggcg cggatggcct ccgggatgcc gccgcaggcc gcgatgtggt tgtccttgac
      841 caggatctgg tcgtagagac cgatccggtg gttatgcccg ccccccagga ggacggccca
      901 cttgtcgagg gctcgcagcc caggcaccgt cttgcgcgtg tcgaggagcc tcgtcgggcg
      961 gtcgtcgatc gcgtcgacca tggcccgcgt cgccgtcgcg atcccgctca tgcgctgcag
     1021 caggttcagg gccacgcgct cgcccgagag gagagcgcgg gccgggcccg cgaccgtccc
     1081 gaaccgacgg ccggccgcca cgcgggcccc gtcgtccaag gtccaggtga gggagacccc
     1141 gccgtcgagc gcgcggaaca cgcggttcac cacctcgatc ccggcgacga tcccgggcga
     1201 cttggccagg aattccgcct cggcgagcgc ctccggcgga atggtcgcac gggtcgtcac
     1261 gtcgcccgac ccgatatcct cggcgagcgc gcgccggacg aaatcgtcca gctccacgtc
     1321 ggtcaggtag ggaggaagca tgcgggtcag gaggggaggg cgagccgctc gagcgcgtcc
     1381 cggagcatgc ggggcgcccg aacggggcgt cccgtcgcaa ggtccaggaa gcacagggtc
     1441 acgtgcccgg agaccaggac gtccggctcg ccctcgcgcc gcacgtcgta gtcgaaccgg
     1501 atgcgcgcga ccggcgcctc gccggtccac gtcaccccga tctcgatggc gtcgtcgtag
     1561 cggaccgggc ggtggtaccg gaccgcgagg tccaccacgg gcatgctcac gccgccctcc
     1621 tcgatctcgc gatacgagat cccggagctg cgcagcgcct ccgtccgggc ggcctcgaac
     1681 cagtccaggc agtggacgtg gtagaccagc cccatggggt cgacttcgcg gtagcgcacc
     1741 cggtgcggat ggacatggcg gggcatgggg accgggtcac tcggtttcgt accgccccat
     1801 ggcgtcgaac ttttccaggc gggcctggcg caggtcgccg ggcgcgatgc cgtcgagcgc
     1861 cttgaggttc gcggcgatcg acgcaccgac cgcctcgtac gcggcgtccg gatcccggtg
     1921 cgccccgccg acgggctcgg cgatgatctc gtcgatgatg ccgaggggga ccaggtccgt
     1981 ggccgtcagc ttcagggcac gggccgcctc ttccttgtag tcccacgagc gccagaggat
     2041 cgacgagcac gactcgggcg atatgaccga gtaccaggag ttctccatca tcaggatgcg
     2101 gtcgccgacg ccgatgccga gcgcgccccc ggaggcgccc tcgccgatga tcacgacgac
     2161 aacggggacg ggcagcgcgg acatctcgac caggttccgc gcgatggcct ccgcctggcc
     2221 gcgctcctcg gcctccatgc cgggataggc gccgggcgtg tcgagcagcg tcacgaccgg
     2281 cttgccgaag cgtgccgcca tccgcatcag gcgcagcgcc ttgcgatagc cctcagggtt
     2341 aggcatcccg aagcggcgat acttccgcat cttggtgtcc cgccccttct gctggccgat
     2401 gaccatgacc gtgcggtcgc ggtaccccca gcgggagccc tggaacgtgg cgagcccgcc
     2461 taccatggcc ggatcgtcgc cgaacagccg gtcgccgtgg agctcctcga atcccgtggt
     2521 ccacgcgtgg atgtggtcca gcgaatacgg ccgcatcggg tgccgcgcga tctgcacccg
     2581 ttgccaccgc gtcaggttgc ggtagatggc cccgcgcagc tcctccaccc gctcctcgag
     2641 cgagcggatg ttggcctgca ggtcagggcc ggactcgtcg gactgaaggc cgcgcatctc
     2701 ctcgagccgg gcctcgagct cgatcagcgg gcgctcgaag tcgagcggct ggggcgttga
     2761 gggtttcgcc atgggttccg cggcgcgggc cgcaggtcag cgtgctgtga gcgttcggat
     2821 gatgatctcc cagtcgaggg cggcggactg gatcgtgaca tagtatccgt aggccctgcc
     2881 cagttcgtcc gtggccgggt tccgaccctc caggacatcc gcaatgttaa acaccccttc
     2941 tttcaggcgc caatccggcg ggacgagacc cgcgtgatcg gggtgcacgc ccaccaggga
     3001 aaggcggccc gagagtacgg acggaagctg caccaggcgc cgcagcgccc gggccgcccg
     3061 cccgtctcga ccgccggcca gggcggccgg aaggacgaag ggaacgagca ggaggcctcc
     3121 gacggccgcg gggatctcga acgcgcgacg ggccgcggtg gtccgcacgc ggacggcgtc
     3181 ggagacgctc gcggtgagtt cgggcacggc gagttccgac acgctggcct tcccgatcac
     3241 gtgcgaacgc ccctcggcga gcatccggaa ctccacgggc aggtcgcgca gcgactggat
     3301 tacctcgaag atgtgctggt tcgagaggtc ccgagtggcg aacacgacgt tgtccacgcg
     3361 gtgcaggcgc acgaggtcgc gcaggtgatg aagcgccccg agactccggc ttccggcctc
     3421 accatcgccg ccgggtgcct cggccggttc gtccggatca acgtacccga tcaggcggaa
     3481 cgggggacgc ggatgggcac gcagcatgcc gtccaggcgg accgcctcgt ccatcggccc
     3541 gacgaggagc gccaggcgcg gctccgaggc gcgggcctgc ctcgcgagcc ggcggagcga
     3601 gagcaggagc gctccgaccg gaagggatac cgccacgacc gcccgcgaaa acgcgatcgc
     3661 ggggacgaag aacgaggtcg ccgccacgag caggaagccg atcgccgctc ccgcgagcac
     3721 cggccgcacg cgatgctccc aggatctccg gtacccgccc gtgagcgcga tgccgagcac
     3781 ggttcccgcg gcgaacgccg gcgccacggt gacgaggaag agcgcatgga ccgccgtgtc
     3841 gggcgccagc gaccggatcc atcccgcggc cgctaccacg acgtacatgg ttgcgaaatc
     3901 ggcgaggggc ccggccatcc tgcgggcggc tccggtgagc agcgagatcg tggcccggac
     3961 cacgatcgcc gcctggagca tcgcgatgaa gattccggag taccgcccgt gcaggtgctt
     4021 cctggcgaag cgcaccatgg cgccgtagaa cagccgcacg tagcggaact cgcccttctt
     4081 ggtactctcg cccttgtagt ggatgatgcg ggtagcgggc gtgtaatgga tgctccatcc
     4141 ggcctgctgg atccggaagc acaggtccag gtcctcgccg tacatgaaga aggcctcgtc
     4201 gaagaggccg gccccgcgcc gcccgggatc cgccgggtcc ccgaagagcg ccgcacggcg
     4261 gaccagcatg caggagccgc tcagcgcgtc gacctcggcc tcctcgtccc gggagaggaa
     4321 cgtcatgttg tagcgcccga acaccgggct ctgtggaaag agcctgccca gcccggacat
     4381 ccggaagaag gccacgcggg gcgtcgggaa ggcccggcgg ctctccgggg cgaaggtccc
     4441 gtccggattc aggatctggc agccgacggc cccggcgccc ggatgttcgt ccatgaagcg
     4501 gacgagcgtg tccagggtgt cctcctggac gatcgtgtcc gggttcagga tcaggacata
     4561 ccgtccccgc gcaagctcca gggcctggtt gttggcggcg ccgaatcccg cgttccgatc
     4621 gttggggacc aggcggacgc gcgggaagcg cgaccgcacc atgggcacgg agccgtccac
     4681 cgagttgttg tccaccacga tcacctcgac ccgcaggtgc cgggcggccc gttcgacgga
     4741 gcgcagcgcc tgggcgagga actcccggac attgtagttc acgatgacca ccgagaggtc
     4801 cggcgcgtcg accgtggccg gcgtgggcga gggtatgtcc gctcgttcgg tgggcatgcg
     4861 gcgaagatac gcccggcccg cggccccgtg tgccggcccg ggaacggccc gggcgcgtgc
     4921 caccggctcg gaactggtcc gtatcatgcg cccgccgttc ggccgccacc gctccatgtc
     4981 ccgcgattcc gcataccgga ccgaggtccg caccctgctc ctgctggcgg gccccgtcgt
     5041 gctgacgcag ctggcccagg tctcgctggg cttcgtggac acggtgatgg tggggcgcct
     5101 cggcgccgcg cagctcgcgg gagtggccct cggcagcacc gtcttcttca ctctcatcat
     5161 catgtgcatg ggcatggtgc ttgcggtggg ccccatggtc tcgcaggcct acggtgccgg
     5221 cacggacgag cccatcggcc ggagcgtgcg gcagggccag tggctcgccc tgatgctggc
     5281 ggcaggattc acgctcgtgg tctggaacgt ccggccggtg ctgctcgcgc tcggccagga
     5341 cccctcgacc gtggacctcg cggtctcgta cctgcgggcg atcgtctggg gcgcgttccc
     5401 gttcctgggc ttcgtgggcc tgcgctcgtt cgtcgaaggg gtttcgcggc cgcggcccgt
     5461 gaccgtcatc gcgttcgtgg gcgtcgggac gaacgtcttc gcgaactgga ccctcatgta
     5521 cgggaagctc ggcttccccg ccctggggat cgtgggcacc gggtgggcga gcgcgatcgt
     5581 cttctggctc gacttcctgc tgctgctcgc gtacgtggcc tcgcagtcgg aattccgtcg
     5641 cttcaggata ttcgagaagc tgggccggcc cgaccggacg tacttccgcg agctcttccg
     5701 catcggatgg ccgatcggtg cgtcgttcgg aatcgagtcg agcatgttca tgatcacggt
     5761 catgatgatg ggctggatcg gcacgacggc gctcgcggcg caccaggtcg cgctccagtg
     5821 cgcgtccttc acgttcatgg tgccgctggg catcggcatc gcgacgtccg tccgtgtcgg
     5881 ccaggaagtg ggccggggag atcgcgaggg cgtcctgcgg gccgggcgcg tcggtctcgg
     5941 gctgtcggtc ctgttcatga gcctggcggc gctctccttc tggctcgtgc cccgcgccat
     6001 cgtgggactc tacctggacc tggacgcccc ggcgaacgct cccgtcgtgg ccctggccgt
     6061 ggcgctgctc caggtcgcgg cggtcttcca ggtcgccgac ggcatccagg tgacggcggc
     6121 cggggcgctg cgcgggctga aggacacccg ggtgccgatg gccatctcgg cggtttcgta
     6181 ctgggccgtc ggcctcaccg gggcatggtt cctggccttc cgcctgggct ggggagcgac
     6241 cggcctgtgg tggggcctgg tgctgggact ggccgccgcc gcggcgatgc tcctcgccag
     6301 gttccggtcg atggcgacct ccggaccggc gatcgccccg gcgtttgatt gaaaattccg
     6361 ggtaccccgg cgtgcatggc caaggggcgc gccgtatgtt ttgcgactct gttaatcgag
     6421 tcccccgggc attcctaccc ctgtccatgg ctcagatttt cccgaagcgc gccaaccggt
     6481 tgcccgcggt ttcgatcgtc ggtctgctcg gtggcgggat cttcgtgatc ttcttcgtct
     6541 ggtactactt ctcacccgag tacacggacg tcgggtatgc gcccgagcag cccgtggcct
     6601 acagccaccg cctgcacgcg gggcagctgg gcgtcaagtg ccagtactgt cacaactggg
     6661 tcgagacggc cccgcacgcc aacgtgccgt ccacgcagac ctgcatgaac tgccacggcc
     6721 agatccggac ggagtcgctc aagctgcttc ccgtccgcga aagctgggcc acggggacct
     6781 cgatcccgtg ggtgaaggtg caccagcttc ccgagtacgt gcacttcgcg cacaacgtcc
     6841 acgtgaccaa cggcgtcggc tgcgagacct gtcacggacg agtcgaccag atggacgtgg
     6901 tgtcgctcgt ggaaccgctc tccatgggat ggtgccttga gtgccaccgg cagcctgagc
     6961 tgtacctgcg ccccgacgac cgggtgacga ccatgggcta cgttcagccc gcggacttcg
     7021 tgcagcgcaa cctggagcgg gttcgcacag agaacattca gccgcccacg aactgctccg
     7081 cctgtcacta ctaggagacg gactgccgtc cctgcggagg ccggcccgtt gccggcaccc
     7141 cgcgacctga aaccgaccgg aagatgatcg agcttcccgt actgcccacc atgccttcag
     7201 cggacggcgc gccgcggtcc cggttctggc gaagcgtcca gcagctcgag ggagacccgg
     7261 cctaccgggc gttcaccgga agcgagctgc tgcccgatgc ctcggggccg ccctcggaga
     7321 cttcccggcg ccagttcctg caggtcatgg gggcctccat ggccctcgcc ggcctgaccg
     7381 cctgccgcaa gccggtcgag gccatcatgc cgttcgcgcg caagccggag gacctggtcc
     7441 ccggcgtgcc gatgcattac gcgacgggca tggagttcag gggtgcgctg cggccgctcg
     7501 tggtcgagtc cacggacggg cgcccgacca aggtcgaggg caacccggag catcccgagt
     7561 ccggcggatc cacgggcgtg ttcgagcagg cgtcgctcct ggcgctgtac gatccggacc
     7621 ggtcccgtgc cgtcctccgc gacgggaacg actcgcactg gaacgccttc gtccgcttcg
     7681 ccgccgagcg gcacgcgggc agcgcctcgg ggcgcctcgc ggtgatcacc cgcccgtcct
     7741 catccatgac ccgagcggcg atccgccggg agatggaagc ggcctggccg ggcctgcgct
     7801 gggtggaata ccaggcggtg cctgacgccg ccgcccgggc ctacgggctc ggcctcggga
     7861 cggccgtcca cccgcggttc cagttcgaga gagcgcttgt cgtcctgagc ctggacgggg
     7921 acttcctggg cgcgacggac atcgaccacg tgcacaacac ccgcgggttc gcccggagcc
     7981 gcgcggtgga ccagaccggg gagatgagcc gcctgtacgt ggccgagagc gcctacacgc
     8041 tcacgggcgg cgcggccgac catcgcctcg cggtgcgccg ctccgatctg ccggccctgg
     8101 ccgcggccat cgcgcggcga ctcggcgtcg aggccggccg gggcggaagt gcctgggaca
     8161 gcgagccgtg ggtcgcggcg gtcgccgacg accttcgtgc cgcgggaccc cgcgcgctcg
     8221 tggtggccgg cgatgacgac gaggtgcagc tcctggccgt cgccatgaac cgggtgctgg
     8281 gcgcgatcgg cggcacggtc gaggtgctcg acaccgggat gccggccggt gggggagccg
     8341 atgcgctcga tgcgctcgtc gccgacatgg ccgcaggccg gatcgacacg ctgctcgcgc
     8401 tcgatgcgaa tcccgtgtac gacctccccg cggccctggg cttcgcgcag gcgctgggcc
     8461 aggtcgccga gaccgtgcac gccggcctgt acgtggacga gacggcccgc gcatgccgtt
     8521 ggcacctgcc gcttgcgcat tacctggagg cgtggtccga cgggcgctcg gcaggcggca
     8581 cccggtcggt ggtccagccg ctgatcgccc cgctgtacga ggccgcgcgg tcggacatcg
     8641 agatcctggg cgttctcgcg tccggcctcg accgcgacgg gtacgaactg gtccgttcga
     8701 cgtggcagcc gctgctcggc ggcggcttcg atgcggcctg gaggcgcgtc gtgcacgacg
     8761 gcttccagcc cgactcggcc tacggcacgg tgcagccctc ggaagccgtg gaggccgcgg
     8821 tcgcctccgc cggcgcggac ggcgcgttcg acgcggggct gcaggacggg atcgagatcg
     8881 tgttccacgc cgattcgaag gtgttcgacg gctcgttcgc caacaacgcc tggctgcagg
     8941 agctgcccga ctcgatctcg aaggtcgtct gggacaacgt ggcgctcgtg agcccgtcga
     9001 cggccgcctc gctcggactg cgggtcgatc tcgtgaacgg gaagcaccgg gtggaccgcg
     9061 tgtcgatctc gacgccggac ggctcggtgg acctcccggt ttggatcgct cccgggctcg
     9121 cccggagctc cgtccacgtg tccctcggat acggccgcaa catcacgtcg tcgcgcccga
     9181 cgcgtcccgg ccacgtgttc gacctcgacc accggaccga cctttacggc cagggggcga
     9241 tctcctctgg cgtgggcgtc tccgtcgcgc ccatccgccc ggggaacctg ggcatggtcg
     9301 tgcgcgaggg ggtgacggtc acgcgctcgg gcggcgacta cctggtggcg tccacgcagg
     9361 accacggcgc gctcccggag gagggcgccg aggccatgaa ccgcgggctg taccgcatcg
     9421 cgacgctcga ggagtaccgc gcgaacccgg acttcgtcgc cgactccgag ccgaagccca
     9481 tccgcgagga ctggggcgac tacccgtcgc tgtggcagaa cgcccacccg gccgaccagc
     9541 cggcgtcgaa ggacaatccg tactcggcga accagtgggg catggtcatc gacctgaacg
     9601 cgtgcacggg ctgcaacgcc tgtctcgtgg cgtgccaggc cgagaacaac atacaggtcg
     9661 tgggacagga ccaggtgagc cgcggccgcg agatgcactg gatccggctg gaccggtact
     9721 tcgtgggcga cggtgccgac ggctcgccgc ggatggccgt gcagcccgtg ccgtgcatgc
     9781 actgcgagaa cgcgccgtgc gagtcggtct gcccggttgc cgccacggtg cactcgcccg
     9841 acggcacgaa ccagatgatc tacaaccggt gcatcggaac gcgctactgc gcaaacaact
     9901 gtccgtacaa ggtgcgccgg ttcaattact acaactggac caagacgctg ccggattcgc
     9961 tgcacatggc gcagaatccg aacgtcacgg tgcgctcgcg cggcgtgatg gagaagtgct
    10021 cgtactgcat ccagcgcgtc cgcgaggcca accggcgctc gagcgtcgag aaccggccca
    10081 ttcgcgacgg cgaggtggtg acggcctgcc agcaggcctg tcccgcggga gccatcgtgt
    10141 tcggggacct ggcggatccg gagagccgcg tctcgtccgc gaggaagaac ccccggcgct
    10201 acgagctgct cgccgagctc tccgtcaagc cccggaccag ctacctgggc cgcatacgca
    10261 atcccaaccc gcgcctggag cgggaagcct gatccggccg cccgcgcaac cgaatccccg
    10321 atcatccccg acgccccgtg agcacggtac cccatagcgc ccaggcggcc cacgcgatcg
    10381 acgatccgcc cctgttcggg tccgacctgt cgttccacga cgtcaccgag ctggtctcgt
    10441 accacaccga gaagaagact ccgacggcgt ggttcggcgc gttcgccctc gcgaacgcgg
    10501 gcctcctgct gctggtgctc atgatcgcct acctgttctg gaacgggtcg ggcgtctggg
    10561 gtctgaacaa cccggtgggc tggggatacg ccatcgtcaa cttcgtcttc tgggtcggca
    10621 tcgggcatgc cggcacgctg atatcggcga tcctcttcct cttccggcag ccgtggcgga
    10681 cgggaatcaa ccggtcggcg gaggcgatga cgatcttcgc cgtcatctgc gcactcgtct
    10741 tcccgacgat ccacgtcggg cgcgtctggg tgatctactg gacgctcccc gtgccgaacc
    10801 agatggagat gtggccgcag ttcaagagcc cgcttctctg ggacgtgttc gctgtttcgg
    10861 tgtatttcac cgtctccctg atgttctggt acgtgggact ggtgcccgac ctcgcgtcgc
    10921 tgcgcgacag ggcgcgcacc ctgtggcgca ggcgcgtgct cggattcctg tccctcggct
    10981 ggacgggcgc caaccggcac tggcgcaact acgagaaggc ctacctgctg ctggccgcgc
    11041 tggccacgcc gctggtgctg tcggtgcact cggtcgtgtc gttcgacttc gccgtttcga
    11101 tcgtgcccgg ctggcacacg accatcttcc ctccctactt cgtcgcgggt gccatcttct
    11161 cgggcttcgc gatggtggtg acgctgctgg tcatctcgcg gaaggtgtac ggcctcgaga
    11221 acatcatcac cgtgaatcac ctggagaaga tgaacatcat cattctcctc acgggcacga
    11281 tggtgggctt cgcgtacatc accgagttct tcgtcgcgtg gtactcgggc gtcaagtacg
    11341 agcagtatgc gttcctgaac cgggccttcg gtccgtacgc gtgggcgtac tggatcatga
    11401 tgtcctgcaa cctccttttc ccgcagctct tctggttccg gaagctgcgc cgctcgatcc
    11461 cgttcatgtt cgcggtctcg atcacgacga acatcggcat gtggttcgag cgcttcgtga
    11521 tcaccgtgac gtcgcttcac cgcgactacc tgccgagctc ctgggactac tactcgccca
    11581 cggccgtgga catcctgacc ttcgtcggga cgttcgggct gttcttcacc ctattcctgc
    11641 tcttcctgcg cttcgtgccc atggtggcca tggccgaggt gaagtacgtg atgccgcagg
    11701 ccgaccccca cttctacgag ggtcacggcg acggacgggc cggagaggac gagccggcgg
    11761 tggttccctc gatccccccg acctcctgat cctcagcaca ggagcgacat gagcacactc
    11821 aaatcagtca tcggcgacgt gagggcgtcc atgggcatct tcgatggccg ggatgccggg
    11881 ctctacgggc tgctggccga gttccgcaac cccggcgcac tgtacgaggc ggccggggcg
    11941 gtccggaaag ccgggtaccg gcgcttcgac gtgcacagcc cgttcccgat tcacggcatg
    12001 gaccgggcga tgggcctggg caactcgcgc gtcgggtggt tcacgctcct gggcggcgcg
    12061 tccggcctgg cgctcggcgt gtggctgcag tggtggacgg gatcggtcga ctacccgctc
    12121 aacatcagcg gcaagccgtt cttcgccgta gagccgtcga tccccatcat tttcgagctc
    12181 acgattctct ttg
//
LOCUS       DBNHQZ010000238         3792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3456875, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000238 DBNHQZ010000000
VERSION     DBNHQZ010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3456875"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC16YU_06350"
     CDS             complement(<1..339)
                     /locus_tag="AC16YU_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064879.1"
                     /translation="MSKSTTSGRVARTSAAGQTNGSPSNGHIPEIMVDVRRHLHRHPE
                     LGLHEYETSRFIRELLERHGLHVHRPLARTGLYVDIVGAHPGPMAAWRADIDALPIQD
                     AKSTPYASTVA"
     gene            complement(456..2612)
                     /locus_tag="AC16YU_06355"
     CDS             complement(456..2612)
                     /locus_tag="AC16YU_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064880.1"
                     /translation="MSLTFGLSPFVLVALVAAAGLAAWWMYRQTVPQLPAGIRIVLGA
                     IRFVVLATILFLLAEPLLRRAVDREQPPVLAVLVDASGSMALSPDEGGSVSAADQARR
                     VVEALRPGLEGVDVRWFGFGAELVDLGGAPDPDSLRFGMGRTDIADALSTASQRLADQ
                     GLTSVILVSDGRHNSGRNPLSVAERFSVPVHTVSVGDTLSRADVQVRSVETNDLAYRG
                     RELPFRATIRHEGFGGRTVDVQLQENGVTIDRATLTLPDGDGATPVELKTVPGAAGLR
                     RYTVSVTVLDGELTGRNNSASVAVRVLESQRRILVLAAGPSPDLASLRTLLERDPDLA
                     VETRVQKSASEFYEGPLPDLDPYDLIVLVGYPGRGASPVDVDRIAAAAGQGTPIFFVL
                     DRRTDQQALARMAAVLPATPETVRPGFTEARVLPTAEGRRHPVLMAEVPAGADPWSVV
                     PPLLVPDSRFAAAPDAQILAVPEIRGIGLDDPVLVTRRRSGTRSAALLATGFWRWSNL
                     PDDLDSFSDAWPGTVERLVHWLVAPEDDRPVRVRPVSESFEGGTEVEFTGQVYDEALV
                     PVPDGAIELRVTTPDGRTLPYTLRPVGAGRYAGSLGVLPEGTYTYAARGLRDDALLGE
                     DSGTFAVGPLSVEFMDPGSDPALLRQVSARSGGVAVDAARAASLVDSLGGRGLLRPSV
                     RTAEVTFRLWQRFPFLALVLVLLTLEWFLRKRRGLV"
     gene            complement(2619..>3792)
                     /locus_tag="AC16YU_06360"
     CDS             complement(2619..>3792)
                     /locus_tag="AC16YU_06360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR6064881.1"
                     /translation="GPLESGLNGSGVVLGLIDTGTGDLMHPATRHLVDSGRILGQVDF
                     SGQPEDDPSTHARMVLSVAAGFDEGELIGPAWGADILLARSEYTPTETNQEEDAFVAA
                     MEWMEAQGADVVNVSLGYTTFDAGEDSYTYEDMDGDTAVTTVASDLAVQHGVVVVTSA
                     GNEGACAGPTSCWYYIGSPADGDSVITAGGIRADSTRYLASSWGPTVDGRTKPDVAAM
                     GTGVRIASGTGYSNGSGTSFSSPMVAAAAVLLLQVRPELTPIEIRDILRTTASQAQNP
                     DNGLGWGIINAEAALALATRVEDPALPSAVEIEAWPNPFADRLTIRVAEPEPGRPVRI
                     ELYDLLGRSVWRHEGTPAPGGMLVLDLDGRTLPSGVLVYRVRHGGAIVTGSLVHLR"
BASE COUNT          563 a         1306 c         1408 g          515 t
ORIGIN      
        1 ggcgacggtc gaagcgtacg gggtcgactt ggcgtcctgg atcgggaggg cgtcgatgtc
       61 cgcgcgccag gccgccatcg gtccggggtg cgcgcccacg atgtccacgt acaggccggt
      121 gcgcgcaagg ggacgatgga catgcaggcc gtggcgctcg agcagctccc ggatgaaccg
      181 ggacgtctcg tactcgtgca ggccgagttc cgggtgccgg tgcaggtgcc ggcgcacgtc
      241 caccatgatc tcgggaatgt gcccgttcga tgggctcccg ttcgtctgcc cggccgcgga
      301 cgttcgtgcg acccggccgg acgtggtcga cttgctcatg cgctgcggtt cctccgcgcc
      361 tgccccgatg gctgtacggc gcgaatatac ccatttgtcg agggatcgac cgtgatcggc
      421 cgcatgaaag gtgctcgcgg accgcgcgag tgggctcaga ccaggccccg ccgcttgcga
      481 aggaaccact ccagggtcag gaggaccagg acgagggcca ggaacgggaa ccgctgccag
      541 agccggaagg tgacctccgc cgtccgcacg gaggggcgca ggagtccccg cccgccgagc
      601 gaatcgacga gagaagccgc gcgggcagca tcgaccgcga caccgccgga acgcgctgac
      661 acctggcgaa gcagggccgg gtccgacccc gggtccatga actcgaccga cagcggcccg
      721 accgcgaagg tccccgagtc ctcgcccagc agggcgtcgt cgcggagtcc gcgggcggca
      781 tacgtatacg tcccctcggg gagaacgccg agcgagccgg cataccggcc cgcgccgacg
      841 gggcggagcg tgtacggcag ggtccggccg tcgggcgtcg tgacgcggag ctcgatcgcg
      901 ccgtcgggga cgggaacgag ggcctcgtcg tagacctgcc ccgtgaactc gacctcggtg
      961 ccgccctcga aggactccga aacggggcgg acgcgcaccg gtcggtcgtc ctccggcgcg
     1021 acgagccaat gaacgaggcg ttcgacggtg ccgggccagg cgtcggagaa ggagtccagg
     1081 tcgtccggga ggttgctcca ccgccagaac ccggttgcca gcagcgccgc cgagcgcgtt
     1141 ccggatcgcc ggcgggtcac caggaccggg tcgtccagcc cgatgccgcg gatctccggt
     1201 acggcgagga tctgcgcgtc cggcgccgcc gcgaagcggc tgtccgggac caggagggga
     1261 gggacgaccg accacgggtc cgccccggcc gggacctccg ccatcaggac ggggtggcgc
     1321 cgtccctcgg ccgtgggaag gacccgcgcc tcggtgaagc ccgggcgcac ggtctccggg
     1381 gtggcgggga gtacggcggc catgcgcgca agcgcctgct ggtccgtcct gcggtccagc
     1441 acgaaaaaga tcggcgtgcc ttgcccggcg gccgcggcga tgcggtccac gtcgacgggg
     1501 gaagcccctc tgcctggata ccccaccagg acgataaggt cgtacgggtc gaggtcgggt
     1561 agcggcccct cgtagaattc cgaggcgctc ttctggacgc gcgtttcgac cgccaggtcc
     1621 gggtcgcgct cgaggagcgt ccgaaggctc gcgaggtccg gagacggccc ggcggccagg
     1681 accaggatgc gccgctgcga ttccaggacc cgcaccgcga cggacgccga gttgttgcgt
     1741 cccgtgagct cgccgtcgag cacggtcacg gagaccgtgt accggcgcag gccggccgcg
     1801 cccggtacgg tcttcagctc gacgggcgtg gcaccgtcgc cgtcgggcag cgtgagggtc
     1861 gcccggtcga tcgtgactcc gttctcctgc agctggacgt cgaccgtccg tccgccgaaa
     1921 ccctcgtggc ggatcgtggc gcggaagggg agctcgcgtc cccggtaggc caggtcgttg
     1981 gtctcgacgc ttcgcacctg tacgtctgcg cgcgacaggg tgtcgccgac cgagacggtg
     2041 tgcacgggaa cggaaaaccg ctccgcgacc gacagcgggt tgcggccgga attgtgcctt
     2101 ccgtccgaga ccaggatgac gctcgtgagc ccctggtccg ccagccgctg cgaggcggtc
     2161 gagagcgcat cggcgatgtc ggtccgtccc atcccgaagc ggagggaatc gggatccgga
     2221 gcgccgccga ggtcgacgag ctcggcgccg aagccgaacc agcggacgtc gactccctcc
     2281 aggccgggcc ggagggcttc gacgacgcgt cgtgcctggt ccgcggcgga gacgctgccg
     2341 ccctcgtcgg gggagagagc catgctgccg gaggcatcga cgagcaccgc gaggaccggc
     2401 ggctgctccc ggtccacggc gcggcgcagg agcggctcgg cgagcaggaa caggatcgtc
     2461 gccagcacga cgaaccgtat ggcgcccagc acgatgcgga tgccggccgg cagctgcggg
     2521 accgtctgcc ggtacatcca ccaggccgcg aggccggccg cggccacgag ggcgacgagc
     2581 acgaacgggg acaggccgaa ggtgagggac atgccggatc agcgcaggtg cacgagtgaa
     2641 cccgtcacga tcgcgccgcc gtgccggacg cggtatacga ggactccgga cggcagggtc
     2701 cgcccgtcga ggtcgagcac gagcatgcct cccggcgccg gcgtgccctc gtgccgccac
     2761 acgctgcgtc ccaggaggtc gtacagctcg atccggaccg gccgaccggg ctccggctca
     2821 gcgacgcgaa tcgtgaggcg gtccgcgaac ggattgggcc aggcctcgat ctcgacggcg
     2881 cttggaagcg ccgggtcctc cacccgggtg gccagcgcga gtgccgcctc ggcgtttatg
     2941 atgccccatc ccaggccgtt gtccgggttc tgggcctggc tcgcggtcgt gcggaggatg
     3001 tcgcggatct cgatcggcgt gagctccgga cgcacctgga gcaggagcac cgcggccgcg
     3061 gcgaccatcg ggctcgagaa ggaggtgccg cttccgttgg agtagccggt gccggacgcg
     3121 attctcacgc cggtccccat ggccgcgacg tcgggcttgg tgcgtccatc aaccgtcggg
     3181 ccccacgaac tggccaggta gcgggtgctg tccgcgcgga ttccccccgc cgtgatgacc
     3241 gaatccccgt ccgcgggcga gccgatgtag taccagcagg aggtggggcc ggcgcaggcc
     3301 ccctcgttgc ccgcggaagt cacgaccacc accccgtgct gcaccgcgag gtccgacgcg
     3361 acggtcgtca ccgcggtgtc gccgtccatg tcctcgtacg tatatgaatc ctcgcccgcg
     3421 tcgaacgtcg tgtagccgag agagacgttc acgacgtccg cgccctgggc ttccatccac
     3481 tccatggcgg ccacgaaggc gtcctcttcc tggttggtct cggtcggggt gtactcgctg
     3541 cgcgccagga ggatgtcggc gccccaggcg gggccgatga gctctccctc gtcgaaaccg
     3601 gcggcgacgg agagcaccat gcgggcgtgg gtgctgggat cgtcctccgg ctggccggag
     3661 aagtccacct gtcccaggat ccggccgctg tcgacgagat gccgcgtcgc cgggtgcatg
     3721 aggtcgcccg ttcccgtgtc gatgaggccg agtacgacgc cggacccgtt cagtccggat
     3781 tcgagcggcc cg
//
LOCUS       DBNHQZ010000239         2920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1291829, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000239 DBNHQZ010000000
VERSION     DBNHQZ010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1291829"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16YU_06365"
     CDS             <1..384
                     /locus_tag="AC16YU_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6064882.1"
                     /translation="EFEEYYRCYGVGRRDVRVMRYNPVAEPHLRYKDVPPGPIPPDRL
                     ARLERDVLAICIALGYDLNTVELAVRDGVPYAIDFMNPAPDADYHSVGDENFEWIVRT
                     MAKFLVEKALEPRRARKFDVRAALG"
     gene            555..1913
                     /locus_tag="AC16YU_06370"
     CDS             555..1913
                     /locus_tag="AC16YU_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064883.1"
                     /translation="MRPSIEPAIRYYHELIQRDLSAAERQLEQLHALQVERKTLFGGR
                     PMSHSLRPTFLSDSMYVDVHDTVYLIRQAVLRVAANFFTDVDRLREEMGLEDWEIELA
                     AIPTDVIRLSANSRMDSFMTADSFKFVEINGESPAGIAYIHELAEIYRELEVFREFTR
                     RHPVRFVSPLQHTIAGLFAVYYDEFGGREEKPAFAIVDHLDVPTIHEFELVKAYLERM
                     GFACEISDPRALETRDGWVYANGRRIDILYRRLLLNEFQAMRDDCRAFLDGYRAQKTC
                     YLNSFRAKMVHKKALFAFLTDARYTHVLTPEQQYAVAAHIPWTRMLREQKTSFRGLSI
                     DLVHFIRANREYFVIKPNDEYGGAGVTLGFAATQSEWDDAIARGLAAPHVVQEKVDIH
                     REPFLVHTDAGWAEVPTVIDLDPYLNGPLLGGCLTRTSSSNLANVTAGGGTLPMFILR
                     YL"
     gene            1918..2769
                     /locus_tag="AC16YU_06375"
     CDS             1918..2769
                     /locus_tag="AC16YU_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR6064884.1"
                     /translation="MPSPLRVAIVDLYDNEPNEGMRAIRELLEAADRAHWDVPLLREV
                     FETRYRGEIPDLSHDVYLSTGGPGSPFAGEGMAWEKTWFDWADAVWRWNAQAPASERK
                     HVLFICHSFQMMVRHFEVASVIPRKSESFGVFPVHPTDAGRSDVLLRGLADPFYAADF
                     RHWQVVQPRAARLAELGASVLAVEKKRPTIELERAVMGIRLSDEMVGLQFHPEADPPG
                     MLRHFRDPVRRESVIGKVGEEKYERIIHRLESPDFLKRTHDAVIPTFLREAVRALRPE
                     AVEAAAA"
BASE COUNT          469 a         1043 c          954 g          454 t
ORIGIN      
        1 gagttcgagg aatactaccg ctgctacggc gtgggccgcc gcgacgtgcg cgtgatgcgt
       61 tacaacccgg tcgccgagcc ccacctgcgc tacaaggacg tcccgcccgg gcccatcccg
      121 cccgaccgac tggcccggct cgagcgcgac gtcctcgcca tctgcatcgc gctcggctac
      181 gacctgaaca cggtcgagct cgccgtacgc gacggcgtgc cgtacgccat cgacttcatg
      241 aatcccgcgc cggatgcgga ctaccactcc gtgggcgacg agaacttcga gtggatcgtg
      301 cggaccatgg cgaaattcct ggtcgagaag gcgctcgagc cccggcgggc aaggaagttc
      361 gacgtgcggg cggcgctggg ataggaccgc gggagcgccg gcttcgggcc cctgagcgat
      421 gggcccggcc ccgcgggccc gggggtgatg ggcccgggag cggcgcctgc ggaactccgt
      481 gttagattgc ttgcgccgca aaccaagttg cacgcccttc caaacgcccg cgggcacatc
      541 ggcccgcccc tcccatgcgt ccgtccatcg agccggccat ccggtattac cacgagctga
      601 tccagcggga cctctcggcc gccgagcggc agctcgagca gctgcacgcg ctgcaggtgg
      661 agcggaagac cctgttcggc gggcggccca tgtcgcacag cctcaggccg acgtttctct
      721 cggactcgat gtatgtcgac gtccacgaca cggtttacct gatccggcag gccgttctcc
      781 gggtcgcggc gaacttcttc accgatgtcg accggctgcg cgaggagatg gggctcgagg
      841 actgggagat cgagctggcc gcgatcccga cggacgtcat ccggttgtcg gcaaactccc
      901 gcatggactc gttcatgacg gccgactcgt tcaagttcgt ggagatcaac ggggaatcgc
      961 cggccggcat cgcgtacatc cacgagctgg cggagatcta tcgtgagctc gaggtcttcc
     1021 gggagttcac ccgccgccac cccgtccgct tcgtgtcgcc cctgcagcac acgatcgcgg
     1081 ggctcttcgc agtgtattac gacgagttcg gcggacgaga ggagaagccg gcgttcgcga
     1141 tcgtcgacca cctggacgta cccacgatcc acgaattcga gctcgtgaag gcgtacctcg
     1201 agcgcatggg cttcgcctgc gagatttccg atccgcgcgc gctcgagacg agggacggct
     1261 gggtgtacgc gaacgggcgc cgcatcgata tcctgtaccg gcggctgctc ctgaacgagt
     1321 tccaggcgat gcgggacgac tgccgggcct tcctcgacgg ctaccgcgca cagaagacgt
     1381 gctatctgaa ctcgttccgc gccaagatgg tgcacaagaa ggccctgttc gcgtttctca
     1441 ctgacgcccg ctacacgcac gtgctcaccc ccgagcagca gtacgccgtt gcggcccaca
     1501 tcccgtggac gcggatgctg cgcgagcaga agacctcgtt ccgcggcctc tcgatcgacc
     1561 tcgtgcattt cattcgggcc aaccgggagt acttcgtcat caagccgaac gacgagtacg
     1621 gcggcgcggg cgtgaccctg ggtttcgcgg cgacacagtc cgagtgggac gacgccatcg
     1681 cccgcggcct ggctgcgccc cacgtcgtgc aggagaaggt cgacatccac cgtgagccct
     1741 tcctggtgca cacggatgca ggatgggccg aggtaccgac cgtgatcgac ctggacccgt
     1801 acctgaacgg cccgctcctg ggcggctgcc tcacgcgcac ctcgtcgtcg aacctcgcca
     1861 acgtcacggc cggcggcggc acgctgccga tgttcatcct gcggtatctg tagtcccatg
     1921 ccgagtcccc tccgcgtcgc gatcgtcgac ctctacgaca acgagcccaa cgagggcatg
     1981 cgggccatcc gcgagctcct cgaggcggcc gaccgtgcgc actgggacgt gccgctcctc
     2041 cgcgaggtct tcgagacccg ctaccgcggc gagatcccgg acctctcgca cgacgtgtac
     2101 ctctcgaccg gcggccccgg cagccccttc gccggcgagg gcatggcctg ggagaagacg
     2161 tggttcgact gggcggacgc cgtatggcgc tggaacgccc aggcgccggc gtcggagcgc
     2221 aagcacgtcc tcttcatttg ccactccttc cagatgatgg tgcgccactt cgaggtggcc
     2281 tccgtcatcc cccggaagag cgagtcgttc ggcgtcttcc ccgtgcaccc cacggacgcc
     2341 gggcgctccg acgtgctgct tcgcgggctg gccgatccct tctacgccgc cgacttccgg
     2401 cactggcagg tggtgcagcc ccgcgcggcg aggctggccg agctcggagc ctcggtcctg
     2461 gccgtcgaaa agaagcggcc cacgatcgag ctcgagcgcg ccgtgatggg catccgcctc
     2521 tcggacgaga tggtgggcct gcaatttcac cccgaggccg acccgcccgg catgctgcgc
     2581 cacttccgcg accccgtccg ccgcgaatcc gtgatcggca aggtgggcga ggagaaatac
     2641 gagcggatca tccaccgcct cgagtcgccc gacttcctga agcgcacgca cgacgcggtc
     2701 ataccgacct tcctgcgcga ggcggtaagg gcactgaggc cggaggcggt ggaggcggcg
     2761 gcggcctagc gcttccccgg cccgacgaac cggaggagac cctcggcggc cctccgcacg
     2821 ggtccgggag ccgtcctcca cgcccacgct accgccgcgg ccaccacgag cgatcgcacg
     2881 gcccatcccg ccggctccag gccagacgta aagaccaccg
//
LOCUS       DBNHQZ010000240         4179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_884714, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000240 DBNHQZ010000000
VERSION     DBNHQZ010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_884714"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="AC16YU_06380"
     CDS             complement(<1..414)
                     /locus_tag="AC16YU_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064885.1"
                     /translation="MPAASQAAAGGPPLDDARLESWREAFRRAGDRDGVRAFLDLMKE
                     AVPGNPAVPGDPAGPTAHAFLATAELMRAGRRGNPVAKLTGFRKWTKVLDRQVRAAPF
                     DPDIRLLRLAVRANAPSFLGYDDGIEADCAAVTGAL"
     gene            complement(468..1319)
                     /locus_tag="AC16YU_06385"
     CDS             complement(468..1319)
                     /locus_tag="AC16YU_06385"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR6064886.1"
                     /translation="MVRNERLELYDEVSRACSRATTRAYSTSFSWSIRMLAPDVRDGI
                     HAIYGFVRLADEVVDSFHGHDREELLARLKGEVARALDERISTNPILQAFQWAFHRYG
                     MERRHVDEFLRSMEWDLHRSQYDRVHFDAYIVGSAEVVGLMCLKVFVRGDRAAYDRLE
                     GPAARLGAAFQKVNFLRDLREDTGELGRRYFPGFDLATFDDGEKRRIEDEIEADLEAA
                     SAGIRDLPRDVRFAVYVAYRLYRRLLSRIRRLPFRAILESRVRVPGGEKLFVIAAASL
                     RHRFNVL"
     gene            complement(1319..2821)
                     /locus_tag="AC16YU_06390"
     CDS             complement(1319..2821)
                     /locus_tag="AC16YU_06390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6064887.1"
                     /translation="MNADRHTDSLVIGAGFAGLAAAVRLARLGHRVTVVERHDEAGGR
                     ARRFESGGFVFDMGPSWYWMPGVFTDFFRMAGSAVADHLDLVRLDPSYRVWFDDGPLD
                     VPAGHDAVRDLFERLEPGAGERLERFMDEAGRKYRLGMEHFVWRPGNSPLEFIDPRIL
                     PDVLRLSLFTSLSAHVRRHFSDPRLVRLLEFPVLFLGVRPERTPALYSLMNYADTVLG
                     TWYPMGGMYRIIDAMVRVARGLGVEFRFGRDVDAILERGGAVAGISSAGTEFGADAVI
                     AAADYHHVEQDLLPSGWRRYDERYWQGRTLSPGSLLFYLGIDRRVDGLLHHNLFFDTP
                     FEPHARTIYDDPSWPEAPSFYVCAPSRTDPSVAPAGCENVFVLVPLAPGLEPDQDQNE
                     RLFQEVMGRIARHAGMDLRPHVVYRRDYAHRDFEADYRAYRGNAYGLANTLRQTAFLK
                     PRMRSRLPGLFFAGQLTTPGPGVPPSLVSGQVAALEADRWLRRRFPGRAA"
     gene            complement(2818..3630)
                     /locus_tag="AC16YU_06395"
     CDS             complement(2818..3630)
                     /locus_tag="AC16YU_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6064888.1"
                     /translation="MDSLLTTTEAARIAGVGVTSIKRWADEDLIRVIRTPGGHRRVVK
                     ADLLEFIRRTSGGTSLPVADEEAVDGERWADAILDDDVLTLQGTLLQARGRLGSWHAV
                     VDDLAHGLREIGRRWERGHVTALAEHVGSERLARALAALTDAIPRSGREPVCLLTCVP
                     GDAHTLGLSLLQLCLREAGWAALWVGQSTPVEDLFAVTGTGQVQMVALTASAASSDPA
                     VLADFLDLVGPACTRYGIRLVMGGAGAWPDPPRYGHRFRTMAQLNAFLVEPK"
     gene            3718..>4179
                     /locus_tag="AC16YU_06400"
     CDS             3718..>4179
                     /locus_tag="AC16YU_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6064889.1"
                     /translation="MNILVTGGTGFIGRALVLRLRRDGHRITVLSRGAARARSLLGTE
                     ADVLESPDADPVPREALERADAVVNLAGEPLMGKRWSDDRKRTILDSRVTMTRAIVEG
                     MRGLPRRPEVLVSASAVGYYGNGGDRVLTEDAGPGDDFLAGVCATWESEARR"
BASE COUNT          669 a         1501 c         1470 g          539 t
ORIGIN      
        1 gagggcgccc gtgaccgccg cgcagtcggc ctcgattccg tcgtcgtacc cgaggaagga
       61 cggcgcgttc gcgcggactg cgagccggag cagccggatg tccgggtcga acggcgccgc
      121 ccggacctgc cggtccagca ccttcgtcca cttgcggaag ccggtcagct tcgcgacggg
      181 attcccgcgc cggccggccc gcatcagctc ggccgtggcg aggaaggcat gggccgtcgg
      241 tccggccggg tcgcccggga cagccggatt gcccgggacg gcctccttca tcaggtccag
      301 gaatgcccgg accccgtccc ggtcgcccgc tctgcggaac gcctcgcgcc acgactcgag
      361 gcgggcgtcg tccaggggcg gcccgcccgc tgcggcctgg gacgcggcgg gcatgggcag
      421 cgccaatgcg gcccacgcca gggcaagaat gcgcgggaag acattcatca gagcacgttg
      481 aaccggtggc gcagcgaggc cgccgcgatt acgaagagct tctcgccgcc cgggacccgc
      541 acgcgggact cgaggatcgc ccggaagggg agcctccgga tgcggctcag aagcctgcgg
      601 tacagccgat acgccacgta cactgcgaac cggacgtccc gcggaaggtc ccggataccc
      661 gcgctcgccg cctcgagatc cgcctctatc tcgtcctcga tgcgccgctt ctccccgtcg
      721 tcgaaggtcg cgagatcgaa gccggggaag taccgccggc cgagctcccc ggtgtcctcc
      781 cgcaggtccc ggaggaagtt caccttctgg aaggccgcgc ccaggcgggc cgccgggccc
      841 tcgagccggt cgtacgcggc ccggtcgccc cggacgaaca ccttcaggca catcaggccc
      901 acgacctccg ccgagcccac gatgtaggcg tcgaaatgca cgcggtcgta ctgcgagcgg
      961 tgaaggtccc actccatgct gcgcaggaac tcgtccacgt ggcggcgctc catcccgtag
     1021 cggtggaagg cccactggaa cgcttgcagg atcggattcg tgctgatcct ctcgtccagg
     1081 gcccgggcca cctcaccctt caggcgggcc aggagctctt cgcggtcgtg gccgtggaac
     1141 gaatcgacca cctcgtcggc cagccggacg aaaccgtaga tcgcatggat gccgtcgcgg
     1201 acgtcgggag ccagcatgcg gatgctccac gagaaggacg tggaataggc ccgcgtggtg
     1261 gcgcggctgc aggcgcggct cacctcgtcg tagagctcca ggcgttcgtt ccggaccatc
     1321 acgcggccct ccccgggaac cggcggcgca gccagcgatc ggcctcgagc gcggccacct
     1381 gtccactgac gaggctcggc gggacgcccg ggcccggcgt ggtcagctgc ccggcgaaga
     1441 acagcccggg caggcggctg cgcatgcgcg gcttcaggaa ggccgtctgg cggagcgtgt
     1501 tcgcgaggcc gtacgcgttg ccgcgatagg cccggtagtc ggcctcgaag tcgcggtgcg
     1561 cgtagtcccg ccggtagacg acgtgcggcc ggaggtccat gcccgcatgg cgggcgatgc
     1621 ggcccatcac ttcctggaag agccgctcgt tctgatcctg gtccggctcc agccccggag
     1681 ccagcggaac gagcacgaac acgttctcgc agcccgcggg cgcgaccgaa ggatccgtcc
     1741 gcgacggcgc gcacacgtag aagctgggcg cctcgggcca cgacgggtcg tcgtaaatcg
     1801 tccgcgcgtg gggctcgaag ggcgtgtcga agaacaggtt gtggtgcagg agcccgtcca
     1861 cccgccggtc gatcccgagg tagaaaagca aggatcccgg agagagcgtc cgcccctgcc
     1921 agtaccgctc gtcgtaacgc cgccacccgg acggaagcag gtcctgctcc acgtggtggt
     1981 agtccgcggc cgcgatcacg gcgtcggcgc cgaactcggt gccggcggag gagatcccgg
     2041 caaccgcgcc cccgcgctcg agaatggcgt ccacgtcccg gccgaaccgg aactcgacgc
     2101 ccagcccgcg ggccactcgg accatggcgt cgatgatgcg gtacatgccg cccatcggat
     2161 accaggtccc gaggaccgtg tccgcgtagt tcatcaggct gtagagcgcc ggggtccgct
     2221 cggggcggac gcccaggaac agcacgggga actcgagcag ccgcaccagg cgcgggtccg
     2281 agaagtgccg gcgcacatga gcgctgagcg atgtgaagag cgacaggcgc aggacgtcgg
     2341 gcaggatgcg gggatcgatg aactcgagcg gcgaattccc ggggcgccac acgaagtgct
     2401 ccatgcccag gcggtacttg cgaccggcct cgtccatgaa gcgctccagg cgctcaccgg
     2461 cccccggctc cagccgctcg aacaggtcgc gcacggcgtc gtggcccgcc gggacgtcca
     2521 ggggaccgtc gtcgaaccac acgcggtagg acgggtccag ccgcaccaga tccaggtggt
     2581 cggcgaccgc ggacccggcc atccggaaga agtcggtgaa gacgccgggc atccagtacc
     2641 agctcggacc catgtcgaac acgaagcctc cgctctcgaa ccgccgcgcg cggccgcccg
     2701 cttcgtcgtg ccgctcgacg accgtcacgc ggtggcccag ccgggcaagc ctgaccgccg
     2761 ccgcgagccc ggcgaacccg gccccgatga ccaggctgtc cgtgtgccga tccgcgttca
     2821 tttcggctcc acgagaaagg cgttgagctg ggccatcgtg cggaagcggt ggccgtaccg
     2881 gggcgggtcg ggccaggctc cggccccgcc catgacgagc cggattccgt accgcgtaca
     2941 ggcgggtccg acgaggtcca gaaagtccgc gagcacggcc gggtccgagg aggcggcgga
     3001 cgccgtgagc gcgaccatct ggacctgccc ggtccccgtc accgcgaaga ggtcctcgac
     3061 gggcgtcgac tggcccaccc agagcgccgc ccagccggcc tcgcgcaggc agagctgcag
     3121 cagggagaga cccagcgtgt gggcgtcgcc cggcacgcac gtgagcaggc agacgggctc
     3181 ccgacccgac cggggaatcg catcggtgag cgccgcgagg gcgcgcgcca ggcgctccga
     3241 ccccacgtgc tcggcaagcg ccgtcacgtg gcccctctcc caccggcggc cgatctcgcg
     3301 aagaccgtgc gcgaggtcgt ccacgaccgc gtgccacgag ccgagccggc cgcgggcctg
     3361 gagcagcgtg ccctgcagcg tgagcacgtc atcgtcgaga atcgcatccg cccagcgctc
     3421 gccgtccacg gcctcctcgt ccgcgaccgg cagagacgtt ccgccggagg tccgccggat
     3481 gaactcgagc aggtcggcct tgaccacgcg gcggtggccg ccgggcgtgc ggatcacgcg
     3541 gatgaggtcc tcgtccgccc agcgcttgat gcttgtgacg ccgacgccgg cgatccgggc
     3601 cgcttccgtg gtggtcagca gggaatccat aatccgaata tagaacgaat tagacgactc
     3661 aacaaaatga aaccgtcaaa accgtctatc ttgggggact gaatatcagg gacggacatg
     3721 aacatcctcg tcaccggcgg caccgggttc atcggccgcg ctctcgtctt gcggcttcgt
     3781 cgcgacgggc accgcatcac cgtgctctcg cgcggcgctg cccgggcccg ctccctcctc
     3841 ggcaccgagg cggacgtctt ggaatccccc gacgcggacc ccgttccccg cgaggcgctc
     3901 gagcgtgccg acgcggtcgt caacctcgcc ggcgagcccc tgatgggcaa acggtggagc
     3961 gacgatcgca agcggaccat cctcgacagc cgggtgacga tgacgcgtgc gatcgtcgag
     4021 ggcatgcgcg gactcccgcg ccggcccgag gtgctcgtct cggcgagcgc cgtgggctac
     4081 tacggcaacg gcggcgaccg ggtgctcacc gaggacgccg gcccgggcga cgacttcctc
     4141 gcaggcgtat gcgccacctg ggagagcgag gcccgccgg
//
LOCUS       DBNHQZ010000241         3065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3323842, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000241 DBNHQZ010000000
VERSION     DBNHQZ010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3323842"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16YU_06405"
     CDS             <1..614
                     /locus_tag="AC16YU_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6064890.1"
                     /translation="LNLIVKADVGGSVEAVADSLLKLSTEEVAVNIVHKGVGAINESD
                     VMLASASDAIIIGFQVRPQPSARALAEAEEIDIRLYSVIYSAIEEVRDALEGLLSPEK
                     SESILGVAEVRETFKVPKVGTVAGCYVTEGKIRRNDRVHLIRDGVVIYEGSLASLKRF
                     KDDAREVVSGYECGMSIENFNDIKVDDQIEAFEIVEKKRKLQD"
     gene            647..1018
                     /gene="rbfA"
                     /locus_tag="AC16YU_06410"
     CDS             647..1018
                     /gene="rbfA"
                     /locus_tag="AC16YU_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR6064891.1"
                     /translation="MSIRTERVARLMQREVADLLVTEFGEQLDRLVTVTGARVTKDLA
                     IAYVYVSVLGDNDVQRQAAFRRLVEFGPRIRQALARRIRHQVRAIPDLRFFLDDTLER
                     AARIDDLLDRINAERARRDEP"
     gene            1008..1748
                     /gene="truB"
                     /locus_tag="AC16YU_06415"
     CDS             1008..1748
                     /gene="truB"
                     /locus_tag="AC16YU_06415"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR6064892.1"
                     /translation="MSRDASRSGDDVRSLVSEHPELPVSWDSAVLPVDKPKGMTSFDV
                     IRVLRRATGIRRMGHAGTLDPMATGLLIILVGRATKRMRDFMEMPKSYAGVIRLGEET
                     PSFDAETEVIRRRDAADVTAADLESARLRFLGPITQRTPSYSAVKVGGERLYRKARRG
                     ERVTAPDRHVTIDRFDLVAWSPPEVAFEVDCSKGTYIRSLAHEFGEALGVGGHLVALR
                     RTGIGPFAVERAWPLGELESRLPGRGGE"
     gene            1757..2698
                     /locus_tag="AC16YU_06420"
     CDS             1757..2698
                     /locus_tag="AC16YU_06420"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR6064893.1"
                     /translation="MERCWSLEEVVRDPLAVVTVGTFDGVHRGHQAVLAYLVERARHH
                     GGHSVVVTFDPHPRTVVHGEPVPLLTTPGERAEVCARSGVDRFIVLPFDEVLAAMTPY
                     GFVRDILFGRIGLTAMVVGHDHAFGRDRSGRHEQLEVMAAELGFRLDTIPPASVGDAV
                     VSSSAIRRILLRDGDVRKAADLLGHRYRLSGHVVAGARRGRTLGYPTANLVPEDARKL
                     VPAEGVYAVSVRDEGRARAWAGMLNIGRRPTFEESEVRIEAHLLDFSGDLYDRRLTVD
                     FIEHLRPERRFPTIEALIAQLREDEARCRSLLKNVSL"
     gene            2775..3041
                     /gene="rpsO"
                     /locus_tag="AC16YU_06425"
     CDS             2775..3041
                     /gene="rpsO"
                     /locus_tag="AC16YU_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR6064894.1"
                     /translation="MISKDSVQELVDQFGKDGKDTGRAEVQVAIFSNRISDLTEHLKR
                     HPNDHSTRRGLLQMVGKRRRLLNYLMSTDIERYRSVIASLGIRK"
BASE COUNT          511 a          986 c         1066 g          502 t
ORIGIN      
        1 agctgaacct catcgtcaag gcagacgtgg gcggttccgt ggaggccgta gccgactccc
       61 tgctcaagct gtcgaccgag gaggtggcgg tgaacatcgt ccacaagggc gtcggagcca
      121 tcaacgagtc ggacgtcatg ctcgcatcgg cctccgacgc gatcatcatc ggatttcagg
      181 tacgcccgca gccgagcgcc cgcgcgctgg ccgaggccga agagatcgac atccgcctgt
      241 actcggtgat ctacagcgcc atcgaggagg tgcgggatgc cctggagggc ctgctctcgc
      301 ccgagaagtc cgagagcatc ctgggcgttg ccgaggtgcg cgagacgttc aaggttccga
      361 aggtgggcac ggtggccggc tgctacgtga ccgagggaaa gatccggcgc aacgaccgcg
      421 tgcacctgat ccgcgacggc gtcgtgatct acgagggatc gctcgcgtcg ctcaagcggt
      481 tcaaggacga cgcgcgcgaa gtggtcagcg ggtacgagtg cggcatgagc atcgagaact
      541 tcaacgacat caaggtcgac gaccagatcg aggccttcga gatcgtcgag aagaagcgca
      601 agctccagga ctgaggacgc ccgaccccga agactcccag ccgaccatga gcatccgcac
      661 agaacgagtt gcccgactca tgcagcggga ggtggccgat ctcctggtca ccgagttcgg
      721 cgagcagctg gatcgcctgg tcacggtgac cggcgcccgg gtcacgaagg acctggccat
      781 cgcgtacgtg tacgtgagcg tgctcggtga caacgacgtg cagcgccagg cagccttccg
      841 gcgactggtc gaattcggac cgagaatccg ccaggcgctc gcccgccgca tccggcacca
      901 ggtgcgcgcg atcccggacc tgcggttctt tctcgacgac accctcgagc gggctgcgcg
      961 gatcgacgat ctgctcgacc gcatcaatgc agagcgtgcc cgccgggatg agccgtgatg
     1021 cctcgcggtc cggcgacgac gtccggtccc tggtgtccga gcacccggaa ctgcccgtct
     1081 cctgggacag cgcggtcctg cccgtcgaca agccgaaggg catgacctcg ttcgacgtga
     1141 tccgcgtcct gcgccgcgcc accgggatcc ggaggatggg gcacgcgggc acgctcgatc
     1201 cgatggccac ggggctgctc atcatcctgg tcggccgcgc cacgaagcgc atgcgggatt
     1261 tcatggagat gcccaagtcc tatgccgggg tcatccggct cggggaggag accccgtcgt
     1321 tcgacgccga gaccgaagtg atccgccggc gcgacgccgc ggacgtgacc gcggccgacc
     1381 tcgagtccgc ccggctgcgt ttcctcgggc cgatcacgca gcggacgccg agctattccg
     1441 ccgtgaaggt cggaggcgag cgactgtacc ggaaggcccg ccgcggagag cgtgtgacgg
     1501 cgcccgatcg gcacgtgacg atcgaccggt tcgacctcgt cgcctggtcc ccgcccgagg
     1561 tcgcgttcga ggtggactgc tcgaagggga cttacattcg gtcgctggcg cacgagttcg
     1621 gcgaggccct gggcgtgggt ggacatctcg tggcgctcag gcggacgggt atcggaccgt
     1681 tcgccgtgga acgggcctgg ccgctcggcg agctcgagtc ccgcctgccg gggcggggcg
     1741 gtgaatgacg atacccatgg aacgctgctg gtcgctagaa gaagtggtgc gggatcctct
     1801 cgccgtcgtg acggtcggga cgttcgacgg cgtgcatcgt ggtcaccagg cggttctggc
     1861 ctacctggtc gaacgggccc gccatcatgg cggacacagc gtggtcgtga cgttcgatcc
     1921 gcatccccga accgtggtac acggggagcc ggtgccgctg ctcacgacgc ccggcgagcg
     1981 ggcggaggtg tgcgcgcgct cgggcgtgga ccgcttcatc gtgctcccgt tcgacgaggt
     2041 gctcgcggcc atgaccccgt acggtttcgt ccgggacata ctgttcgggc ggatcggact
     2101 gaccgcgatg gtggtcgggc acgatcatgc gttcggccgg gaccggagcg ggcggcacga
     2161 gcagctcgag gtcatggcgg ccgagctcgg tttccggctc gacaccattc cgccggcgag
     2221 cgtgggggat gccgtggtgt cctcgtcggc catccgcagg atcctgctgc gggacgggga
     2281 cgtccggaag gccgccgacc tcctcggcca tcggtaccgg ctctccggac acgtggtcgc
     2341 gggggcccgt cgcgggcgca cgctcggcta tccgacggcc aacctcgttc ccgaggatgc
     2401 gcgcaagctc gtgcccgcgg agggcgtgta tgccgtgtcg gtccgcgacg aggggcgcgc
     2461 gcgggcctgg gccggcatgc tgaacatcgg ccggcgtccg actttcgagg agtccgaggt
     2521 ccggatcgaa gcgcacctgc tggatttctc gggcgatctg tacgatcgtc ggcttaccgt
     2581 ggatttcatc gagcatctgc gcccggaacg gcgtttcccg acgatcgagg cgctcatcgc
     2641 gcagctccgc gaggatgagg cgcgttgcag gtccctcctg aagaacgtat ctttataagc
     2701 ttatgtggca gacagccacg accgtctctt gacgggccat tcttctttac gatggtagaa
     2761 tctaaagagg atccatgatc tcgaaagaca gcgtgcagga actggtcgac cagttcggca
     2821 aggacggaaa ggacacgggc cgcgcagagg tgcaggtcgc cattttctcg aaccgcatct
     2881 ccgacctgac ggaacacctg aagcggcatc cgaacgacca ctcgacgcgt cgtggcctgc
     2941 tccagatggt cggcaagcgg cgccggcttc tcaactacct gatgtcgacc gacatcgagc
     3001 gctatcgttc ggtgatcgcc agtctcggca tccgcaagta accaactccg gggcggcatc
     3061 cttcg
//
LOCUS       DBNHQZ010000242         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2780966, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000242 DBNHQZ010000000
VERSION     DBNHQZ010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2780966"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1774
                     /locus_tag="AC16YU_06430"
     CDS             356..1774
                     /locus_tag="AC16YU_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HIR6064895.1"
                     /translation="MRIATTFSNRRDEPRRVRTHRLLTGLLTLLSITAFTSFTSRPGS
                     AQVPYCEDCVRPRPLAAAGGIVSINLVTWSYDRFLARAWWARVGPETWRQNLRGGMVW
                     DDNRLTSNQMMHPLSGAMFFNAARSSGYGYWGSMAGAAFGSVLWEYLGETHPPSPGDL
                     VSTSVGGSALGETMRRFSHALLASRSRPGAGRRLAAGLLDPVAHASNRLGGRVTGGAD
                     LPEHIAVSVDVGFDHALGSAADRTTRFRPVLDVGVQYGDLIRDEVRRPFDVFEFLIRL
                     GRGISSGRVYGPENPVDRLESRGALTAAVLVPDRLAFGVYQHIDFSRSPASEFGAQSL
                     TAAIAGQTRPTGALRWSGAFHLGAMLLGSSPSRLVVEPERVNDYGPGLLGKIDTRLST
                     RRVDLLSLQARMYWMHDVSGSRARHLVTLVAGEARVPLGGGFGIGAAVEVSSHASFDD
                     QLREPRVHAAATRFTVSWSPGR"
     gene            1870..2739
                     /locus_tag="AC16YU_06435"
     CDS             1870..2739
                     /locus_tag="AC16YU_06435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6064896.1"
                     /translation="MYGQDSIEIARDLPPVDAHPLDWELLVCTHVLGLTSLHWGLWTT
                     DSPLTLDSLREAQERYTELILNTVPAGVRSAIDVGCGTGDLARRLAARGCRVKAIAPI
                     ANYEHRLSLDQVPGLSWQRLRLQDLNGDGRYDAVLMIESAGYFPIALGLEQARNHLRS
                     GGWLLVVNPFRTVETEFEVARHGLSDYLDAAREYGFVLDREIDITDAVLPTLHLTRRF
                     RTEHAEPALSLLRFAAMRSRPVRLGLGLASFFFRRRLEAVRRKLKTFREQMDPDLFRE
                     HARYMVLLFRLGG"
     gene            2904..>3259
                     /locus_tag="AC16YU_06440"
     CDS             2904..>3259
                     /locus_tag="AC16YU_06440"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6064897.1"
                     /translation="MPPLAGTASARQTQKPPLHGRHWVAITGKPLAATAGSMMFQQGG
                     NAVDAACAMLAATSTMWDVLSWGGETQALIYNPTTGKVIGINALGVAPTGATPEFFRS
                     RGMDYPPEYGPLAAVTP"
BASE COUNT          454 a         1204 c         1052 g          549 t
ORIGIN      
        1 cgtcggttcg cggtgggcga atccaatcat ccggtttccg cagcggccgc gcaaggccgt
       61 tccgcgctcc ggggggcgac gtggaatacg agaggcgctg tccgatttcc ccgggtcccc
      121 tgtccgattc gtccgggccc gcccgaacgg gtaattcgcc aaaaagtgcg tattcggtgc
      181 cattctgggc cctgaagggc cattctggtg cgccggccgc ctccccgagg cttagcccgg
      241 tccccgtcct ggcctctctg aagggcgtcc gaaaatcacg gaaagtcgac cggttcgacc
      301 gcgcatgtcc gcccgatcgt gtcctcggaa gcacgatcac cagccgagcg gtgccatgcg
      361 tatcgccacc accttctcga atcgtcgcga tgagcctcgc cgtgtgcgga cgcaccgtct
      421 gctcaccggg ctcctcaccc tcctctcgat cacggcattc acttccttca cgtcccgacc
      481 gggatccgcc caggtcccgt actgcgagga ttgcgtccgg cccaggccgc tggccgccgc
      541 gggcggaatc gtctcgatca acctcgtgac ctggagctac gaccgtttcc tggcccgcgc
      601 ctggtgggct cgcgtggggc cggagacatg gcggcagaac ctgcggggcg gcatggtctg
      661 ggacgacaac cggctcacca gcaaccagat gatgcacccg ctcagcggcg ccatgttttt
      721 caacgcagcc cggtccagcg gttacggcta ctggggctcg atggccggcg ccgccttcgg
      781 cagcgtgctc tgggagtacc tgggcgagac ccatccgccc tctcccggag atctcgtcag
      841 cacgtccgtc ggcggatcgg ccctggggga gacgatgcgt cgcttctccc acgcgctcct
      901 cgcctcccgg agccggccgg gggccggacg gcggcttgcg gccgggctac ttgatcccgt
      961 ggctcacgca tcgaatcggc tcgggggccg cgtcaccggg ggcgccgacc tgccggagca
     1021 catcgccgtc tcggtggacg tcgggtttga ccatgcgctg ggttccgcgg cggatcggac
     1081 aacccgcttc cgtcccgtcc tggatgtggg cgtgcagtac ggcgacttga tccgtgatga
     1141 ggtccggcga ccgttcgatg tgtttgagtt cttgatccgc ctgggccggg gcatctcgtc
     1201 gggccgcgtg tacggtccag agaacccggt ggaccggctg gagtcgcgcg gggcgctcac
     1261 cgcggctgtc ctggtacccg accggcttgc cttcggtgtg taccagcaca ttgacttcag
     1321 ccgcagcccc gcctcggagt tcggggccca gagcctgacc gccgccatcg cgggccagac
     1381 ccgaccgacc ggggcgctgc gctggtcagg cgcattccac ctcggtgcga tgctcctggg
     1441 cagcagcccg tcccgcttgg tggtggagcc cgagcgggtg aacgactacg ggccgggcct
     1501 cctgggcaag atcgataccc gtctctccac gcgtcgcgtc gatctcctca gcctccaagc
     1561 ccgcatgtac tggatgcacg acgtatccgg tagccgggca cggcacctgg tcacgcttgt
     1621 ggcgggagag gcgcgtgtcc cgctcggagg cggattcgga atcggggcag ccgtcgaggt
     1681 cagcagccat gccagtttcg acgaccagct ccgcgagcct cgcgtgcacg cggcggcgac
     1741 gcgcttcacc gtttcgtggt cgcccggccg gtgagccggc gccgcacgcc tccaacgact
     1801 ccctgcccga cgggccaacc cgaccagggc ccttcccaaa ccgatccttc atccttccgg
     1861 aggtccccca tgtacggtca ggattccatc gaaatcgcgc gcgacctgcc gcccgtcgac
     1921 gcacacccgc tggactggga gcttctcgta tgcactcacg tcctggggct cacctcgctg
     1981 cactggggac tctggacgac ggactccccc cttaccctgg acagccttcg cgaggcacag
     2041 gagcgttaca cggaactcat cctgaacacc gtgccggccg gagtccgctc cgcgatcgac
     2101 gtcggatgcg gcaccgggga cctggcgcgc cgcctcgccg cccgcggctg ccgcgtgaag
     2161 gccatcgcgc cgatcgcgaa ctacgagcac aggctctccc tggaccaggt gcccgggctt
     2221 tcgtggcagc ggctccgcct gcaggacctg aacggggacg gacgctacga cgccgtgctc
     2281 atgatcgagt ccgcggggta cttcccgatc gcgctcggcc tcgagcaggc gcgcaatcat
     2341 ctccggtctg gaggttggct gctggtggtc aacccgttcc ggacggtgga aacggagttc
     2401 gaggtggccc gccacggatt gtccgattac ctcgacgcgg cccgcgagta tggcttcgtc
     2461 ctcgaccggg aaatcgatat caccgatgcg gtgttgccga cactccacct gacgcggcga
     2521 ttccggaccg agcacgccga accggccctg tcgctcctcc gctttgccgc catgcggtcg
     2581 cggcccgtgc ggctcggcct cggcctcgca tcgttcttct tccggaggcg gctggaggcc
     2641 gtccggcgga agctgaagac ctttcgggag cagatggatc cggacctgtt ccgcgagcac
     2701 gcgcgataca tggtgctgct gttccggctt ggtgggtgat cctgctccgt cgctccggtc
     2761 caggaccagc ccattggaca ccttgcctcg cccaccctcc gtgtctacgt ttccgggccc
     2821 acccgcgccg cgccgcgcgg cgcaccgcgt ccgagacccg cccacccatg tcccgcctcc
     2881 cgctcctcgc cgccctcctc ctcatgcccc ccctcgccgg caccgcctcc gcccgacaga
     2941 cccagaagcc gcccctgcac ggccggcact gggtcgcgat cacgggcaag ccgctggccg
     3001 cgaccgccgg ctcgatgatg tttcaacagg gcggcaacgc ggtcgacgcg gcctgcgcga
     3061 tgctcgccgc aacgtccacc atgtgggacg tcctctcctg gggcggcgag acgcaggcgc
     3121 tgatctacaa cccgacgacc ggcaaggtca tcggcatcaa cgccctcggg gtcgcgccca
     3181 cgggagcgac cccggagttc ttccggagcc gcggaatgga ctacccgccg gaatacggtc
     3241 ctctcgccgc cgtcacccc
//
LOCUS       DBNHQZ010000243         3920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2916135, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000243 DBNHQZ010000000
VERSION     DBNHQZ010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2916135"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..443
                     /locus_tag="AC16YU_06445"
     CDS             48..443
                     /locus_tag="AC16YU_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6064898.1"
                     /translation="MTVRSRTLTKKDVARRVADLVGEPIYKCEPWVGSVVDAIGQLMM
                     EADPEIRIEMRDFGVFEVKKTKAKPKARNPKTNETVFIPSRRKTHFKPSKKLKEVLQQ
                     PLQELNYAVPEGSADYSDVPAGLSGVAAD"
     gene            470..895
                     /gene="ruvX"
                     /locus_tag="AC16YU_06450"
     CDS             470..895
                     /gene="ruvX"
                     /locus_tag="AC16YU_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6064899.1"
                     /translation="MGDGRIVGIDYGTKRVGIAMADPLRMFAQPVGTFDPDEAVRRLK
                     AIHSGEGIGTVVIGWPLEEDGREGDSTRRVDAYVRRLRNALGRIEVVRWDERYTSEEA
                     RDRLAGRGSVHGGRVDTAAAGLILQEYLDSRARAPGEPE"
     gene            931..1503
                     /gene="def"
                     /locus_tag="AC16YU_06455"
     CDS             931..1503
                     /gene="def"
                     /locus_tag="AC16YU_06455"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6064900.1"
                     /translation="MILPIHVYGDPVLREQTIPLDGDSAELQALIDSMIETMRGAEGI
                     GLAAPQVGRTERVFVVDLAPLAEPDGGNGVAWPPQPMVFINPVIEEESDEECDYEEGC
                     LSIPDLREIVVRPERITVRYLDRTFTPRILEADDVLARVIQHEYDHLDGVLFTDRITP
                     FRRRLLQRRLREMARGEVTADYPLALAGTV"
     gene            complement(1513..2313)
                     /locus_tag="AC16YU_06460"
     CDS             complement(1513..2313)
                     /locus_tag="AC16YU_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR6064901.1"
                     /translation="MTDLLAYPFMQRALAGGVLVGMLASYFGVFVVQRRLSFLGAGLS
                     HAAFGGVALGLLLRAEPLAVAVPFTVLVSLGINWVRRHTRLAGDTAVGVFFAVSVALG
                     LVFLALRRGITVDAFTYLFGSVLSVSATDVALAAAMLLVGLATLPRLWGRWAYASFDE
                     ESARAERLPVDRDDYLFFTLLAVTIVVSVKVVGIILMAAFLVIPAASARLVSPTFRHM
                     TIVAVGIGVLSSLAGLVISYLFDVPSGATIVLVQAAVFFGLILASRRR"
     gene            complement(2310..3059)
                     /locus_tag="AC16YU_06465"
     CDS             complement(2310..3059)
                     /locus_tag="AC16YU_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR6064902.1"
                     /translation="MTDAVETDGLRVTLGDRDVLQDITFRLPSGSMLAVLGPNGSGKS
                     TLLRTFVGLVEPSGGSFRILGCARGELACGAVGYVPQIKSLDRVFPARAVDLVLTAAL
                     GRWPGRISRASRILAEEALDRVGARHLADRSIGALSGGELQRIYLARAVSRDPAVLLL
                     DEPEAGVDAVGTADLHSMLDRYRADHGTTIVLVTHDWDAAQFHATHVLLLRGRQIAFG
                     PTGEAFTEEQLRRAFGHVGHAHPARHDGPVP"
     gene            complement(3056..>3920)
                     /locus_tag="AC16YU_06470"
     CDS             complement(3056..>3920)
                     /locus_tag="AC16YU_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR6064903.1"
                     /translation="PFLTGRAVRAAALALLLQASCGREPVRETGSVTVVTTFHALATV
                     IAPVVGPAGSVHVLLPGGASPHAYEPRPSDLRELSRANLLFYAGPDVDGWASRLASTP
                     AVALAGLLPDSLLAGSEAGSDPHLWLDPVLVAAALPGLADSLCSVDETNCAGYRERAR
                     SFAASTPGLVLRIRDTLGAASGGSAVVSGPFLAWWSLRFGPRVAGIIEESEGVEPSAA
                     RIRAMIDTARGADLVIGQATLPDEAARAVAESASIPYVTVDAVGTPATAPDYERLLLA
                     IAAAIAQPRTP"
BASE COUNT          693 a         1284 c         1457 g          486 t
ORIGIN      
        1 ccgttacttg atatctcctt ggattcacgc ggtttactgc cagcaagatg acggtccggt
       61 ccagaaccct gacgaagaag gacgttgcac ggcgcgttgc cgacctggtc ggtgagccga
      121 tctacaagtg cgagccgtgg gtgggttccg tcgtggacgc catcggccag ctcatgatgg
      181 aggcagaccc cgaaattcgc attgaaatgc gggatttcgg ggtgttcgaa gtcaagaaga
      241 cgaaggccaa gcccaaggcc cgcaatccga agacgaacga aacggtcttc attcccagcc
      301 gccgcaagac gcatttcaag ccgtcgaaga agctcaagga agtgctgcag cagccgctcc
      361 aggagctcaa ctatgccgta ccggagggca gcgcggacta ctcggacgtg cccgccggcc
      421 tgagcggcgt ggcggccgac tgagcgttgc cgccctcgct cctcgcacca tgggtgacgg
      481 gcgaatcgtc gggatcgact acggcacgaa gcgtgtgggc attgccatgg ccgacccgct
      541 gcggatgttc gcgcagcccg tcggcacgtt cgatccggac gaggccgtgc gaaggctgaa
      601 ggccatccac tcgggggaag gcatcggcac cgtggtcatc ggatggccgc tcgaggagga
      661 cggccgcgag ggagactcca cgcgccgcgt cgacgcgtac gtccgccggc tgcggaatgc
      721 cctgggtcgg atcgaggtcg ttcgatggga cgaacggtac acgagcgagg aggcccgcga
      781 tcggcttgcg ggacgcggat cggtccacgg ggggcgggtg gacacggcgg ccgccggcct
      841 catcctgcag gaatacctgg acagccgggc ccgcgcgccg ggggaacccg aatgagccct
      901 ttcggtaggt gacggggcaa tatgaagacg atgattctcc ccatccacgt atacggagac
      961 ccggtactcc gggagcagac gattccgcta gatggcgaca gcgcggagct gcaggcgctc
     1021 atcgattcca tgatcgagac gatgcggggc gccgaaggta tcggtctcgc ggcgccgcag
     1081 gtcggtcgca cggaacgggt gttcgtcgtg gacctcgcgc cgctcgccga gccggatggg
     1141 ggcaacggag tcgcctggcc gccccagccg atggtcttca tcaacccggt gatcgaggag
     1201 gagtcggacg aagagtgcga ctacgaggag ggctgcctgt ccataccgga cctgcgcgag
     1261 atcgtcgtac ggcccgagcg gatcaccgtc cgttacctcg acaggacgtt cacgccccgg
     1321 atcctggaag cggacgacgt gctcgcacgc gtcatccagc acgaatacga ccacctggac
     1381 ggggtcctgt tcaccgatcg catcacgccg ttccggcggc gactcctgca gcgccggctt
     1441 cgggagatgg ctcgcggcga ggtgacggcc gattacccgc tcgcgctggc cggcacggtc
     1501 tgacccgttc cgtcagcggc ggcgggaggc gagtatcagg ccgaagaaca cggcggcctg
     1561 gaccagcacg atcgtggcgc cgctcggcac gtcgaagagg tacgagatga cgaggcccgc
     1621 gagcgaggag aggaccccga tgccgacggc cacgatggtc atgtgccgga aggtgggcga
     1681 aaccaggcgg gccgacgcgg cggggatgac caggaaggcg gccatcagga tgatgcccac
     1741 caccttgacc gagacgacga tcgtgaccgc gagcagggtg aagaacaggt agtcgtcccg
     1801 gtccaccggc aggcgctcgg cgcgcgccga ctcctcgtcg aacgaggcgt atgcccaccg
     1861 gccccacagc ctggggagcg tcgcgaggcc gaccaggagc atcgcggcgg caagggcgac
     1921 gtcggtcgcc gagaccgaga gcacggagcc gaaaaggtag gtgaaggcgt ccacggtgat
     1981 gccgcgccgc agggcgagga acacgaggcc gagggcgacg gaaacggcga agaacacgcc
     2041 caccgccgta tcgccggcga gtcgcgtatg acggcggacc cagttgatgc ccagcgacac
     2101 gagcaccgtg aacggcaccg cgaccgcgag gggctcggcc cggagcagga ggcccagggc
     2161 cacgccgccg aacgcggcgt gcgagaggcc ggcaccgagg aagctcagcc gtcgctgcac
     2221 gacgaacacg ccgaaatagc tggccagcat gcccacgagc acgccgcccg ccagggccct
     2281 ctgcatgaac ggataggcaa ggaggtccgt catgggacag ggccgtcgtg ccgggcgggg
     2341 tgtgcgtgcc ccacgtgccc gaaggcccgg cggagctgct cctccgtgaa ggcctccccg
     2401 gtcgggccga aggcgatctg ccgcccccgg aggagcagca cgtgcgtcgc gtgaaactgg
     2461 gcggcgtccc agtcgtgcgt caccaggacg atggtggtgc cgtggtccgc gcgataccgg
     2521 tcgagcatcg aatgcaggtc ggccgtgccg acggcatcca cgccggcctc cggctcgtcg
     2581 agcaggagaa cggccggatc gcgcgagacg gcgcgggcca ggtagatgcg ctgcagctca
     2641 cccccggaaa gcgcgccgat ggaccggtcg gccaggtgcc gggccccgac ccggtccagg
     2701 gcctcctcgg ccaggatcct ggacgcccgg gatatgcgcc ccggccagcg ccccagggcc
     2761 gccgtgagca ccaggtccac ggcgcgtgcg gggaacacgc gatcgagcga cttgatctgc
     2821 gggacgtaac ccaccgcgcc gcaggcgagc tcgccgcgcg cgcatcccag gatccggaac
     2881 gagccgccgg acggctctac gagtccgacg aaggtcctga gcagcgtcga cttgccgctg
     2941 ccgttggggc cgaggaccgc gagcatcgat cccgacggca gccggaacgt gatgtcctgc
     3001 agcacatcgc gatccccgag ggttacccgg agcccatccg tctctacggc gtcggtcatg
     3061 gggtgcgggg ctgggcgatg gccgcggcga tggcgagaag caggcgttcg tagtcgggcg
     3121 ccgtggcggg cgttcccacg gcatcgacgg taacgtacgg aatcgacgcg gactcggcga
     3181 ccgcccgggc cgcctcgtcg ggaagcgtgg cctggccgat gacgaggtcc gcgccccgtg
     3241 ccgtatcgat catggcgcgg atgcgggccg ccgacggctc gaccccctcg ctctcctcga
     3301 tgatgccggc gacccgcggg ccgaaccgga gggaccacca ggcgaggaaa ggccccgaaa
     3361 ccacggccga gccgcccgac gcggcgccca gggtgtcccg gatccgaagg accaggccgg
     3421 gggtcgacgc ggcgaatgag cgcgcgcgct cccggtaccc tgcgcagttc gtctcgtcca
     3481 ccgagcagag ggagtcggcg agaccgggaa gggcggccgc caccaggacg ggatcgagcc
     3541 agaggtgtgg gtccgacccc gcttccgaac cggcaaggag ggagtccggc aagaggccgg
     3601 cgagagcgac cgccggggtg gaggcgaggc gggacgccca cccgtcgacg tccggcccgg
     3661 cgtagaacag gaggtttgcg cggctgagtt cgcggaggtc cgaggggcgc ggctcgtacg
     3721 cgtgcgggct ggcgccgccc gggagcagca cgtgcacgct tccggcaggt ccgacgaccg
     3781 gggcgatcac ggtcgcgagc gcgtggaagg tcgtgacgac cgtgacggac cccgtctcac
     3841 ggacgggttc gcggccgcag gaggcctgca gcagcagggc gagggctgcg gcgcgtacgg
     3901 cgcgtccggt gaggaacggc
//
LOCUS       DBNHQZ010000244         4310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1155348, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000244 DBNHQZ010000000
VERSION     DBNHQZ010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1155348"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16YU_06475"
     CDS             <1..663
                     /locus_tag="AC16YU_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064904.1"
                     /translation="VLCAAWLAVLPWAGEGWVWILDLLRDPLGMSDRTAAVHYVLGGR
                     TVLVLPEAIIDGPEPSTPTVVLNAVALLVLLIVSFVISVEKAPLRYFLRLIVLLHGMS
                     VIFHALPGRELPYDVGGYVSGLLAAGVTVIALVPIVLSLTHWLFDFGLARKLGLAVGV
                     MAYLYVLIPFKYALHAIILHVGGMIYLPILFVLFGLPIEVMIFIAFYGWGMSWKGRTP
                     SN"
     gene            738..3812
                     /locus_tag="AC16YU_06480"
     CDS             738..3812
                     /locus_tag="AC16YU_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064905.1"
                     /translation="MNPLARVALGCLLAVSALGAPDAHAQASDWTLSLRSGDTFYIDT
                     PKDLFGMYTAVALVNGSASDWQNVTATISLDAGNVQLAQYEDGTVEIGDVAAGQTVMA
                     YFYLTASLETTVPQSFTVTVGYEDEFGAAQPAASDDFAFVAVEETIAANANKVETVVS
                     MPDIPTAGAPLIITVTGRTGELGLSRVLSYTPAAYAGWPADALQLVAVEIAFSGANNE
                     TWYDRLVGTLFDSSNTDYVATYVFRIDQGFAEPTAVSPIGYIASGTQIKHTDTGGFGS
                     LPPIEPPVVPDHIDLELGVVPDNLNPDEGSTVTYTITVTNEPIPGRDPFTATGVEVFA
                     FIPPGTPVYPDAYLGGVPNGQASQGTFNFGTGDWNVGTLAVGESATLELILYVPYPNE
                     LETYAGTSQLFWAEVSAANEPDVDSTPGNYPIHEDDDGEVNLAPSSSGGEGGIESNGT
                     MAGQLAQLLFDRRRASGSTDAQVLKPTPYSAATVAGKTTPGTESAPFARLATEILRGV
                     IPVGGPASSTAYETSPRDLLPITNADDIVAVDFVRDADGRRIGAFFGALTTGELYEHT
                     KVICDRLRGGVLEVVGRTTVAGRPFLISRIDHEDGTVDYAISMVAVRAGSGFVVDSRF
                     RLDEYEAPVASDEVMNLQVWSQNEAWTVQLAETVIHRLQQAGPVRFRNEAYAAPDVPG
                     VYVRTGRYDRGSLELELFNGAGVDHVRLTGGSYSLSERGERIEFEREVLLPAADENGI
                     VRVNLPLGPVFDATFFVETPDGGRDQMYLADGAWSYTFDGTGARVGDFDVAPASEAAR
                     KEGFLGVERAATLAGDVKTWAVMYRYLRANGQPVDLSDYGYVTFTASGTGQVRVLFEK
                     ASVHTSDHFGTTIKLTEEPQTYRLWYDDLRRADGTGRLSAEDLVLVSFYVYGDQSRVR
                     PFELKVGDLQFGGGPGDPLAEVPDQYELKQNYPNPFNPQTQITFGVPTSGDVRLAVYD
                     VLGRQVATLVDGFKPEGRHTVTFDASRLASGMYLYRLEAGTHRVTRMMTLLR"
     gene            complement(3919..>4310)
                     /locus_tag="AC16YU_06485"
     CDS             complement(3919..>4310)
                     /locus_tag="AC16YU_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6064906.1"
                     /translation="TFTAFSDSTPAAVDPSADQETVDAAKSPDVHATATVDRMPELIG
                     GLKSVAESIIYPAIAKEAGIQGRVVVEFVVDKTGAVTDAKVVSGIGGGCDEEAVRAVQ
                     AASFIPGMLDGEPVAVRMALPVSFKLQ"
BASE COUNT          637 a         1548 c         1454 g          671 t
ORIGIN      
        1 gtgctgtgcg ccgcctggct cgccgtcctt ccgtgggcgg gcgagggctg ggtatggatc
       61 ctggacctgc tccgcgatcc gctgggcatg tccgaccgga cggcggcggt ccactacgtc
      121 ctcgggggcc gcacggtcct ggtgctgccc gaggccatca tcgacggccc cgagcccagc
      181 acgccgaccg tcgtcctgaa tgccgtggcg ctgctggtcc tgctcatcgt ctcgttcgtc
      241 atctccgtgg agaaggcgcc cctgcgctac ttcctgcgcc tgatcgtgct gctgcacggc
      301 atgtcggtca tctttcacgc cctgcccggc cgcgagctgc cctacgacgt gggcggatac
      361 gtgtccggcc tcctcgcggc gggggtcacc gtgatcgccc tcgtccccat cgtgctttcg
      421 ctcacgcact ggctcttcga cttcggcctg gcccgaaaac tgggcctcgc ggtcggcgtg
      481 atggcctacc tgtacgtgct catcccgttc aagtacgcac tgcacgccat catcctgcac
      541 gtgggcggta tgatctacct gcccatcctc ttcgtcctct tcggccttcc catcgaggtg
      601 atgatcttca tcgccttcta tggatgggga atgagctgga agggacgcac gccttcgaac
      661 tgatcgtacc cgaggaagac accaccatga atgctacgac ccttgtaccc cccaccgatt
      721 gcgcgcgggg ccgggcattg aacccactcg cccgggtcgc gctcgggtgc ctcctggccg
      781 tttcggccct cggagcgccc gacgcgcacg cccaggcctc cgactggacc ctgtccctgc
      841 ggtccggcga caccttctac atcgacacgc ccaaggacct cttcggcatg tacacggccg
      901 tagcgctcgt gaacggctcg gcgtcggact ggcagaacgt cacggcgacg atctcgctcg
      961 acgccggaaa cgtgcagctg gcccagtacg aggacggcac cgtggagatc ggcgacgtcg
     1021 cggccggcca gaccgtgatg gcgtacttct acctcacggc gtcgctcgag acgaccgtgc
     1081 cgcagtcgtt cacggtcacg gtgggctacg aggacgagtt cggcgcggcg cagccggccg
     1141 cgtcggatga cttcgcgttc gtcgccgtcg aggagaccat cgccgcgaac gcgaacaagg
     1201 tcgagaccgt ggtgtcgatg ccggacattc cgacggccgg cgccccgctc atcatcacgg
     1261 tgacgggccg cacgggcgag ctcgggctca gccgcgtgct ctcctacacg ccggcggcgt
     1321 acgccggatg gcctgccgac gcgctgcagc tcgtggccgt cgagatcgcg ttctcgggcg
     1381 ccaacaacga gacctggtac gaccggctgg tcgggacgct gttcgactcg tcgaacaccg
     1441 attacgtcgc gacctacgtg ttccgcatcg accagggctt cgcggagccg actgcggtga
     1501 gcccgatcgg ctacatcgcg agcggcacgc agatcaagca cacggacacc ggcgggttcg
     1561 ggagtcttcc cccgatcgag ccgcccgtgg tgcccgacca catcgacctg gaactgggcg
     1621 tcgtgcccga caacctgaac ccggacgagg gctcgacggt cacgtacacg atcacggtga
     1681 cgaacgagcc catcccgggc cgcgatccct tcaccgcgac cggcgtcgag gtcttcgcct
     1741 tcatcccgcc cgggacgccc gtgtaccccg acgcctacct gggcggcgtg cccaacgggc
     1801 aggcctcgca gggcacgttc aacttcggga ccggggactg gaacgtgggc accctggccg
     1861 tcggcgagtc ggccacgctg gagctgatcc tgtacgtccc gtacccgaac gaactcgaga
     1921 cctacgcggg caccagccag ctgttctggg ccgaggtgag cgcggcgaac gagcccgacg
     1981 tggactcgac gccgggcaac tacccgatcc acgaggacga cgacggagaa gtgaacctcg
     2041 cgccgtcgtc gagcggcggc gagggcggca tcgagtcgaa cggcacgatg gccggacagc
     2101 tcgcgcagct gctcttcgac cggcgccgcg cctcgggctc gacggatgcc caggtcctca
     2161 agcccacgcc gtacagcgcc gcgacagtcg ccggcaagac gacgccgggc accgagtccg
     2221 ctccgttcgc gcggctggcc accgaaatcc tccgcggcgt gatcccggtg ggcgggcccg
     2281 cgagcagcac ggcgtacgaa acgtcgcccc gcgatctgct tccgatcacg aacgccgatg
     2341 acatcgtggc ggtggacttc gtccgcgacg ccgacgggcg gcggatcggg gccttcttcg
     2401 gggccctgac gaccggcgag ctctacgagc acacgaaggt catctgcgac cgcctccgcg
     2461 gcggcgtgct cgaggtggtg ggccgcacca ccgtcgcggg ccgtcccttc ctcatctccc
     2521 ggatcgacca cgaggacggg acggtggact acgcgatcag catggtcgcg gtccgcgcgg
     2581 gctccggttt cgtcgtcgac agccgcttcc gcctggacga gtacgaggcg ccggtggctt
     2641 cggacgaggt catgaacctg caggtgtggt cccagaacga ggcctggacc gtccagctgg
     2701 ccgagacggt cattcatcgg ctgcagcagg ccggcccggt gcgcttccgg aacgaggcgt
     2761 acgcggcccc ggacgtgccc ggcgtatacg tccgcaccgg gcgctacgac cgcggctcgc
     2821 tcgagctcga gctcttcaac ggcgcgggcg tggaccacgt ccgcctgacc ggcggcagct
     2881 actcgctctc cgagcgcggc gagcggatcg agttcgagcg cgaggtgctg ctcccggccg
     2941 ccgacgagaa cggcatcgtg cgcgtgaacc tgccgctcgg gccggtcttc gacgcgacgt
     3001 tcttcgtcga gaccccggac ggcgggcgcg accagatgta cctggccgac ggcgcctgga
     3061 gctacacctt cgacggcacc ggggcgcgcg tgggcgactt cgacgtcgcg ccggcctcgg
     3121 aggccgcccg gaaggagggc ttcctgggcg tcgagcgcgc ggccaccctc gccggcgacg
     3181 tcaagacgtg ggccgtgatg taccgctacc tccgcgccaa cgggcagccg gtggacctgt
     3241 cggactacgg gtacgtcacg ttcacggcct cgggcaccgg ccaggtgcgc gtgctcttcg
     3301 agaaggcctc ggtccacacg tccgaccact tcgggacgac gatcaagctg accgaggagc
     3361 cccagaccta ccggctctgg tacgacgacc tgcgccgtgc cgacggcacc ggccgcctct
     3421 cggccgagga cctggtgctg gtgtcgttct acgtgtacgg cgaccagagc cgcgtgcggc
     3481 cgttcgagct gaaggtcggc gacctgcagt tcggcggcgg cccgggcgac ccgctcgcag
     3541 aggtgccgga ccagtacgag ctgaagcaga actatccgaa cccgttcaac ccccagacgc
     3601 agatcacctt cggggtcccg acctcgggcg acgtgcgcct cgcggtgtac gacgtcctgg
     3661 gccggcaggt ggcgaccctc gtcgacgggt tcaagccgga aggccgtcac acggtgacct
     3721 tcgacgcatc gcgcctggcc tcgggcatgt acctgtaccg gctcgaggcg ggcacccacc
     3781 gggtgacgcg gatgatgacg ctgctccgct gatcccggtc cgtcacgggc acaaaaggga
     3841 agggcgctcc gcctcggcgg ggcgcccttc gtccattccg ggactcccgg tgccgcgtcg
     3901 ctcccgcgcg gccggcgcct actgcagctt gaagctgacg ggcagcgcca tccggaccgc
     3961 gacgggctca ccgtccagca tgcccgggat gaagctggcg gcctgcacgg cccgcacggc
     4021 ctcctcgtcg cagccgccgc cgatgccgct gacgaccttc gcgtccgtga ccgcccccgt
     4081 cttgtccacc acgaattcga ccacgacgcg tccctggatg ccggcttcct tcgcgatcgc
     4141 cgggtaaatg atggactcgg cgacggactt caggccgccg atgagctcgg gcatccggtc
     4201 gaccgtggcg gtggcgtgga cgtcaggact cttggccgcg tcaacggtct cctggtcggc
     4261 cgacgggtcg acggccgccg gggtggagtc gctgaaggcc gtgaaggtca
//
LOCUS       DBNHQZ010000245        16687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1155416, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000245 DBNHQZ010000000
VERSION     DBNHQZ010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 16687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16687
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1155416"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1748)
                     /locus_tag="AC16YU_06490"
     CDS             complement(306..1748)
                     /locus_tag="AC16YU_06490"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6064907.1"
                     /translation="MKLAFRLTVLSFMGAGLTPSPAASQVVTGDSLALVAIHQALDGY
                     AWTTRTGWLTEPVGSWFGITVEGNRVTEIDLGRNGLRGQIPSGIGTLDALRVLRLDGN
                     DASRGYVTGPLPPEIGNLRQLVELDLSGLPYLEFPYPPEFWDLLALEILDLRGAGVTE
                     GRIPPEIGRLHNLRELWLGGFIYYDLPAELGELANLEHLYIYACHLTGTIPPEFGRLS
                     RLKVLYIDASRCPGGRQLTGPLPAELGNLAELEDLRLLEMWDAGPLPQNWANLGRLKI
                     LGLRNIRLTGPVPHWLGQLRSLEQISLGSNELEGSLPSELANLEGLRSLGVEHNPLSG
                     TLPLSLTALGELGYFDFSDTELCEPDDQAWMEWRSGVATFIPSGCLDEGTAVDPAGAP
                     PDRIAVEAVYPNPASTRTTILYRVPRPSGVSITLIDVAGRTVLTPTSGFHEAGSYELT
                     LDMSGLTPGVYLCVLSAGALAERRTVVLLR"
     gene            complement(1836..2870)
                     /gene="ruvB"
                     /locus_tag="AC16YU_06495"
     CDS             complement(1836..2870)
                     /gene="ruvB"
                     /locus_tag="AC16YU_06495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6064908.1"
                     /translation="MTDRSGDIGAAPLRADEDFEKALRPRRLDEFIGQQQIKDNLTVF
                     MTAAQRRGETLDHVLLSGPPGLGKTTLAFIIAEEMGARIRTTSGPVLDKPASIAGLLT
                     NLNEGDVLFIDEIHRLAPVVEEYLYAAMEDYRIDILIDSGPNARSVKITLPPFTLIGA
                     TTRKGLLTAPLRARFGIDFRYDYYTADLLHRIVERSAGLLGVSISPDGASEIARRSRG
                     TPRIANRLLRRARDFAEVESDGHITTEVADRALNALNVDSHGLDDMDVRILTTLIEKF
                     SGGPTGLGNLAVSVGEDAGTLEEVYEPYLIQEGLIERTPRGRVATARAYRHFHARPPG
                     DRGEDLFGEG"
     gene            2997..3581
                     /locus_tag="AC16YU_06500"
     CDS             2997..3581
                     /locus_tag="AC16YU_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HIR6064909.1"
                     /translation="MFAVGNILISDDLLEAPFACNLGACRGACCVQGDSGAPLEADER
                     ERLEAVLPALRHRLRPEALRVIEEKGTWEEVAPGEFATTCVGGAECVFVIYRGPVASC
                     AIHEAAIEGKVDWPKPISCHLYPIRIEDLGEMEALNYERVGICDPGIRHGRRTGIGLA
                     DYLREPLVRRYGEAWYREFEEAIAERRAALGRTG"
     gene            3588..5069
                     /gene="rpoN"
                     /locus_tag="AC16YU_06505"
     CDS             3588..5069
                     /gene="rpoN"
                     /locus_tag="AC16YU_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6064910.1"
                     /translation="MLNLQQKQSLQQKLSPQQIQYIKLLQLPTLALEQRIKAELESNP
                     LLEEGEEEEELETTTDDQSTDADESEPAEEPADRADEDYDWEELLPDADELYGHKARV
                     DQSDDDRRELPMPARTSMAEHLTDQLSFADFTDDERLVAEQIIGSIDEDGYLRRSLES
                     ITDDIAFNYGVFMEDAGVEQVLRRVQRLDPVGIASRDLRECLLVQLDVLDPATPGRDV
                     AMRMLEDAYKAFTMKHFDQIMKRLEVSGEELKEAYELVQRLNPKPGEGEFSAAQNYIT
                     PDFTVRFEDGDFLISLNQGNTPELRISREYRRMLEQIVSDRKQGKSVSYDSDTRSFLK
                     NKLETARWFINSIQQRRHTMMKVMEALVHLQQDFFKFGEGNLRPMILKDIADIIGMDI
                     STVSRVVNGKYVQCDFGVYELKHFFSEGLATDSGEEVSNKEVKAIIEGIIAAENKAKP
                     LSDQRIAEMLEEKGFQIARRTVTKYREALGVPVARLRKEIVLS"
     gene            5140..6636
                     /locus_tag="AC16YU_06510"
     CDS             5140..6636
                     /locus_tag="AC16YU_06510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6064911.1"
                     /translation="MTSLEAWFAPFRRNTIGFDARFDTPYGRQKLVYADWIASGRLYA
                     PIESRMAHTFGPFVGNTHSESSVTGTSMTRAYHEARRIIKRHVNAGPDDVIIAQESGM
                     TGVVNKLQRILGLRVPERLLPYVSLPESERPVVFITHMEHHSNQTSWLETLAEVVCVD
                     PDDDGLVDPEKLREAVSRHAGRPLIGSFTACSNVTGVRTPIHELARIMHEAGGLCFAD
                     YAASAPYDPIDMHPDDPLERLDAIFFSPHKFLGGPGSAGVMVFDGRLDRNRVPDHPGG
                     GTVDWTNPWGEHRFVENLEDREDGGTPAFLQTIRAALAVRLKEEMNPDRMREREDEML
                     ARAIPALRAVPGLHILADHLEDRLGILSFYLDQVHYNLVVKLLNDRFGVQVRGGCSCA
                     GTYGHYLLHVDRDRSRRITEAIDHGDLSEKPGWVRLSLHPTMTDRELDWTLDAIRQIA
                     GHADEWAADYRYDPHSNEYVHRADVARADAVAAWFEPAVAEQEARAAV"
     gene            6925..7227
                     /locus_tag="AC16YU_06515"
     CDS             6925..7227
                     /locus_tag="AC16YU_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064912.1"
                     /translation="MAAASLRKTSKSLSALFARYVRGEIGDPIWSRLMHLLDADDVER
                     PDRLALARFFNDVVAEGGPGALFVPKDDEARALLVDIRPAVPLRRGPLGDRTASSV"
     gene            complement(7370..8254)
                     /locus_tag="AC16YU_06520"
     CDS             complement(7370..8254)
                     /locus_tag="AC16YU_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064913.1"
                     /translation="MLVASLSAGIRALRARPGLALLLFVVNLAMAFLLAVPIGAALGS
                     ALEGTGFDADLAERLDIVLMTDILVRHGSLFRILAAQLFWIVPVVLAWKAALGVGLVH
                     ALRDDGTGSFWTGVGRYFGRSFGLSLLYVVPVAFVLFVIVLAGVGLDALSGEAGTFRT
                     WTVVVPLFAVIGLAKMDLFQDLGRISIVLRGSGVFAAFVDGIRFALRHWGVFLLYLAW
                     LVPAGLFWLLPTVLEAAIGASLVVFLLQQVLMLGRAGVTVAWIGSEVAFFEGAAEARP
                     EALAAPAEEAAETPVGFA"
     gene            complement(8333..10318)
                     /locus_tag="AC16YU_06525"
     CDS             complement(8333..10318)
                     /locus_tag="AC16YU_06525"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6064914.1"
                     /translation="MFLRFFTAVLLVAALCPRMARAQATTIHDGPLSERRVSYTMDVS
                     LDPARRAVTGRQRLTWRNPDTVPVDELQFHLYLNAFKPGSTFQVESGGVHRGFRAAED
                     DRWGGITVQRMRIASDLPADLAGSLAYPGPEVDLTDRIRFIQPDDGNASDETVIAVSL
                     PRPVGPGETIALDIDFESIMPRITARTGFLEDDDGRPFFFVGQWFPKIGVYEVPGQRY
                     VPADAERGAWNTHQFHANSEFYSDFGTYDVTLTVPDSFVVGATGVRTEERSEGPNRVL
                     RYRADDVHDFAWTAYEGYLVLEDQWEHVAIRLLLRPEHEAQADRPFRAAKIALDRFTD
                     WVGEYPYTTLTLVDGIGGSNGMEYPTLITCGTYYGMPEWFRPVEMVTVHEFGHQYFYG
                     LLASNEFEEAWLDEGMNSYVESRIMDDAYGPGSMLELPGLLVSDSDVQRLSYTRNSPA
                     RGALFTWSWKYRFGDYGKASYSKPATIMNALEGYLGWETMHRFLRTYYERWRFRHPTT
                     RDLQATLEEVAGQDLAWFFDQFVYGTAVVDYAVDAIASTPAPSEGDGPGMFASRVRVQ
                     RVHDGHFPVTIRVRFEDGATEDVRWDGVEEWEEFTFTKASPIEEAYIDPDGILWLETE
                     RLNNRRSTVGSNAFARKERRKFTTRLQQALYVIQALF"
     gene            10423..11316
                     /locus_tag="AC16YU_06530"
     CDS             10423..11316
                     /locus_tag="AC16YU_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6064915.1"
                     /translation="MLPDSVELCEVGPRDGFQFESKPIPTELKVRVIRDLAAAGLRRI
                     QAVSFVHPDRVPQMADAERVAAALPTGTATVFSGLALNARGVLRAADAGLRAVDLSIA
                     TNPVHARDNAGMSVEEGVDMALAMIEEGRRLGLAVQLGLQTVFGYAAPGDTPPELVVD
                     LAARIAAAGVESLSLADSTGLAGPADVRRVVRAVRGAAPGTDLVLHLHDTRGLGLANV
                     AAAIEEGVSRFDTSLGGLGGCPFIPGATGNIATEDTLWLLERLGIRTGIDRAAVAAVT
                     RTVAAHLGRDLPGRLYRLPEA"
     gene            complement(11313..14180)
                     /gene="gcvP"
                     /locus_tag="AC16YU_06535"
     CDS             complement(11313..14180)
                     /gene="gcvP"
                     /locus_tag="AC16YU_06535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR6064916.1"
                     /translation="MAIDLRPTDRFVDRHIGPSEADVAAMLERIGAPSLEALTDQAVP
                     ASIRLGQPLRLPEPRSEREMLADARAMADLNQEWLTVIGMGYSPTVTPPVIQRGILEN
                     PAWYTQYTPYQAEIAQGRLEALLVFQTAIMDLTGMEIANASLLDEGTAAAEAMTMLHR
                     ATRDAARDTFFVDDACHPQTLAVVETRAEPLGIRVVSGDYGTFQPDGRTVGALVQYPA
                     TDGAVRDYRPFCDRVHAAGAHVVVAADLLSLALLEAPGSFGADVVVGSSQRFGVPMGY
                     GGPHAAFFATREAFKRQLPGRIIGVSVDADGNPALRMALQTREQHIRRDKATSNICTA
                     QVLLAVMAGMYAVYHGPDGIRGIATRVHDLARILAAGLERAGLPVAHADYFDTLRIPV
                     DPARRATVRAAATAARINLRYFDDGGVGISLDETTTPGDVGRLLALFGAEADVTAIAA
                     AIPSGYGGSMPRSTPYLTDSVFSAYRSETEMMRYMHRLSSRDLSLTTSMIPLGSCTMK
                     LNAAAELIPITWARFGRLHPFVPVEQAQGYRRLMDDLRAWLGEITGLPGVSLQPNSGA
                     AGEYTGLQVIRAFHRDRGEAHRTVCLIPSSAHGTNPASAVMAGMEVVVVACDERGNVD
                     VDDLRSKAEANADRLAALMITYPSTHGVFEERIGEICDIVHASGGQVYMDGANMNAQV
                     GLCRPGDFGADVCHLNLHKTFAIPHGGGGPGVGPICAAPHLSPFLPGHPVIATGGERA
                     IGPVASAPFGSAGVLFISWAYIALLGEPGLRRATQAAILNANYMAARLEGHFGTLYTG
                     ERGRVAHEFILDLRELRHDSPLTEVDVAKRLMDYGFHAPTMSWPVLGTMMVEPTESES
                     RAELDRFCEAMIAIHGEIQEVLSGAADPADNVLANAPHTAAMVTADTWERPYTREKAA
                     WPAAWVRQHKFWPSVRRVNDAYGDRNLVCSCAPLEAYADQA"
     gene            complement(14272..15024)
                     /gene="rnc"
                     /locus_tag="AC16YU_06540"
     CDS             complement(14272..15024)
                     /gene="rnc"
                     /locus_tag="AC16YU_06540"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR6064917.1"
                     /translation="MLRFLRRALSRLRPERRTHGLDPDSFVRITGEPVERFDLYVQAM
                     KHRSVLRDAVDGDADSNERLEFLGDAVLGLATAEYLYLRYPDEDEGYLTRLRAKLVNA
                     RALASRAEALGLGDLVILSDNMKQARGGSNTNILSDAMEALIGAVYLDQGADAARRFV
                     SSRLLEPLDLDSLAQHEDNHKSLLLEYAQERGWPQPRYEVVSEEGPGHDRRFTVQVII
                     HDDPVGEGTGKSKKIAGQRAAAAALRHLRSGA"
     gene            15161..16537
                     /locus_tag="AC16YU_06545"
     CDS             15161..16537
                     /locus_tag="AC16YU_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6064918.1"
                     /translation="MTVRSVNPYTASEIARYAVLDEDALRGRLRSAAEAFDRHRSTTM
                     EERGGHMHRVADVLEEQVDRWAELMTAEMGKPVVQARAEARKCAWVCRWYADRAPALL
                     ADEPVETDALRSFVAFEPLGPVLAIMPWNFPFWQVFRFAAPAIMAGNVALLKHASNVT
                     GCALAIEEIFRAGGFDRDEFQTLVIPSSVVDRVIEDGVVRAATLTGSNAAGRAVGCAA
                     GRAIKPSVLELGGSDPFVVLADADVAAAARTAVAARMQNNGQSCIAAKRFIVERPVLE
                     AFTAAFCDGVHRLSVGDPMDPSTDVGPLARADLVDEVADQVDRAVKGGAEVLVGGRRG
                     EGPGYFYEPTVLGTVGEESVAFREEIFGPVASVIVAEDADDALRLANRTPFGLGGAVF
                     TADPARGERFARGMQVGCAFVNGMVKSDPRLPFGGIRESGYGRELAGFGIRAFVNVKS
                     IWIAGAPA"
BASE COUNT         2574 a         5649 c         5971 g         2493 t
ORIGIN      
        1 ggctcacgcc tgcgtggccg tcaatacgca ggaccaggtc ttcgtggtgg agggggagaa
       61 ggtggtcgag gcgtggggcg tggatgcgag ggggcgggtg gggtagtcga tcgtcggcgt
      121 tggccgacgt gctccggctg cggttcgtta tgcgaaactg agtatgtcct ccctcggcga
      181 aaccggctgc ggggtcgtaa cccccaaggc aatcccggca tgtgcaggtg taccgggcgg
      241 catggtccat gctcgacgga tgaacgatgg cgagcccggt cgaactccgc accctccgac
      301 gcctcctacc gcagcaacac cacggttcgg cgctcagcaa gggcgcccgc agagaggacg
      361 cacagataca cgccgggagt cagaccggac atgtcgagag tcagctcgta tgagccggcc
      421 tcgtgaaacc cggacgtggg ggtgagcacg gttcgacccg cgacatcgat aagcgtgatg
      481 ctcacgccgg aaggtcgtgg cactctgtac aggatggttg tccgcgttga agcgggattc
      541 gggtacacgg cctcgacggc aatgcggtcc ggtggagcac ctgccgggtc gacggcagtc
      601 ccttcgtcca gacagcccga ggggatgaac gtggcgacgc cgcttcgcca ctccatccag
      661 gcctgatcgt caggttcgca gagttcggtg tcgctgaaat caaagtagcc gagttcgccg
      721 agagcggtga gcgacagggg aagtgtcccc gacagtggat tgtgttccac gcccagagat
      781 cgcagccctt cgagattggc cagctccgac ggcagggatc cctcgagctc gttcgagccc
      841 aggctgatct gttctaggct cctgagctgc ccgagccaat gcggcacggg gcccgtgagc
      901 ctgatatttc ggagacccag gatcttcagt cggcccagat tcgcccagtt ctgtggaaga
      961 gggcctgcat cccacatttc gagcaatcgc aggtcctcca gctcggcgag attgccgagt
     1021 tcggcgggca gcggaccggt aagctggcgc cctccggggc accgggaggc gtcgatgtac
     1081 agcaccttca gcctgctgag ccggccgaat tccggcggga tggtgccggt cagatggcat
     1141 gcatatatgt acagatgctc gaggttggcg agctcgccga gctcggcggg aaggtcatag
     1201 tagatgaagc cgcccagcca gagctcgcgg agattgtgca gtcggccaat ctccggagga
     1261 atccgcccct cggtaacacc cgctccacga agatccagta tctcgagtgc gagcagatcc
     1321 cagaactcgg gcggatacgg gaattccagg tagggaagcc cgctcaggtc cagttccacg
     1381 agttggcgaa ggttgccgat ctccggtgga agcggtccgg tgacgtatcc acgggaggca
     1441 tcgttcccgt cgagacggag cacgcggagg gcgtcgagcg ttccgatccc ggacgggatc
     1501 tgcccccgta aaccgttccg cccgaggtcg atctccgtca ccctgttccc ctctacggtg
     1561 atcccgaacc atgatcctac gggctcagtc aaccaccccg tccgggtggt ccatgcatat
     1621 ccgtccagcg cctggtgaat ggccaccagg gccagggagt cgccggtcac cacctgcgat
     1681 gctgccgggc tgggcgtcaa ccctgctccc atgaacgaga gcacggtcag ccggaatgcc
     1741 agtttcattg ccggccacga aggtctgccc gatgttaccg cagtcctggc aggccgttcg
     1801 cgggaattgc ccacgtgctc cggtagggga cttcctcacc cctccccgaa gagatcctcc
     1861 ccccggtccc ccggcggccg cgcgtggaag tgccggtacg cccgggccgt cgcgacgcgc
     1921 ccgcgcggcg tccgctcgat gagcccctcc tggatcaggt acggctcgta gacctcctcc
     1981 agcgtgccgg cgtcctcgcc gaccgagacg gccaggttgc ccaggcccgt cgggccgccc
     2041 gagaacttct cgatgagcgt cgtgaggatc cggacgtcca tgtcgtccag gccgtgcgag
     2101 tccacgttga gcgcgttcag cgcccggtcg gcgacctcgg tcgtgatgtg gccgtcggac
     2161 tcgacctcgg cgaagtcgcg ggcgcggcga aggagccggt tcgcgatgcg cggcgtgccg
     2221 cggctgcggc gggcgatctc gctcgcgccg tccggagaga tggacacgcc gagcaggccg
     2281 gcggaccgct cgacgatgcg gtgcaggagg tccgcagtgt aatagtcgta gcggaagtcg
     2341 atgccgaagc gggcgcggag gggcgcggtc aggagcccct tgcgcgtcgt ggccccgatc
     2401 agggtgaagg gcggtagcgt gatcttgacg ctccgtgcat tggggccgga gtcgatgagg
     2461 atgtcgatcc ggtagtcctc catcgcggcg tagaggtact cctcgacgac cggggcgagc
     2521 cggtggatct cgtcgatgaa gaggacgtcg ccctcgttca ggttcgttag gagccccgcg
     2581 atggaggcgg gtttgtcgag caccgggccc gacgtcgtcc ggatgcgcgc ccccatttcc
     2641 tcggcgatga tgaaggcgag cgtcgtcttg ccgaggcccg gaggcccgga cagaagcacg
     2701 tggtccagcg tctctccgcg ccgctgcgcc gcggtcatga agaccgtcag gttgtccttg
     2761 atctgctgct ggccgatgaa ctcgtccagc cggcgaggcc ggagcgcctt ctcgaagtcc
     2821 tcgtcggccc ggaggggcgc cgcgccgatg tcgccggatc ggtccgtcat ggtcggggcc
     2881 ggggtttaag aatgcctcat tcctgtcgat acaggccgtt tttggggtac cggggagcgg
     2941 actggcgcgc ccgttccggt tgacaaacag ccatgggctg tctacaataa ggcatcatgt
     3001 ttgctgtggg caacatactg atttccgacg acctcctgga ggcccccttc gcgtgcaatc
     3061 tcggcgcgtg ccggggcgcg tgctgcgtgc agggcgattc gggcgcgccg ctcgaggcgg
     3121 acgagcgcga gaggctcgag gcggtgctcc ccgctctgcg tcaccggctc cgccccgagg
     3181 ccctgcgggt gatcgaggag aagggcacgt gggaagaggt cgcgccgggc gaattcgcca
     3241 cgacctgtgt cggcggggcg gagtgcgtgt tcgtcatata ccggggcccg gtcgcgtcgt
     3301 gcgcgatcca cgaggcggcg atcgaaggga aggtggactg gccgaaaccc atctcgtgcc
     3361 acctctatcc catccggatc gaggacctgg gcgagatgga ggccctgaac tacgagcgcg
     3421 tgggcatctg cgacccgggc atccggcatg gccgacggac gggcatcggg ctcgccgact
     3481 acctgcgcga gccgctcgtg cgccgctacg gcgaggcgtg gtaccgcgag ttcgaggagg
     3541 cgatcgccga gcggcgcgcc gcgctcggca ggacggggta gcgcgccatg ctgaatctcc
     3601 agcagaagca gtcgctgcag cagaagctgt cgccgcagca gatccagtac atcaagctgc
     3661 tgcagctgcc cacgctcgcg ctcgaacagc gcatcaaggc ggagctcgag tccaatccgc
     3721 tgctggagga gggcgaggag gaagaggagc tcgagaccac gacggacgac cagagcacgg
     3781 acgcggacga gtccgagccc gcggaggagc ccgccgaccg ggcggacgag gactacgact
     3841 gggaggagct gctccccgat gccgacgagc tctacggcca caaggcccgc gtggaccagt
     3901 cggacgacga tcggcgcgag ctgcccatgc cggcccggac gtccatggcc gagcacctca
     3961 cggaccagct ctcgtttgcc gacttcaccg acgacgaacg cctggtggcc gagcagatca
     4021 tcggatcgat cgacgaggac ggctacctgc gccgctcgct cgagtcgatc accgacgaca
     4081 tcgcgttcaa ctacggcgtc ttcatggagg acgccggcgt ggagcaggtg ctgcgccggg
     4141 tgcagcgcct ggatcccgtg ggcatcgcgt cccgggacct ccgggagtgc ctgctcgtgc
     4201 agctcgacgt gctcgatccc gcgactcccg gtcgcgacgt ggcgatgcgc atgctggagg
     4261 acgcctacaa ggcgttcacc atgaagcatt tcgaccagat catgaagcgc ctggaggtat
     4321 ccggcgagga gctcaaggag gcgtacgagc tggtccagcg cctgaacccc aagccgggcg
     4381 agggcgagtt ctcggccgcc cagaactaca tcaccccgga cttcacggtc cgcttcgagg
     4441 acggcgattt cctgatctcg ctcaaccagg gcaacacccc ggagctgcgc atctcccgtg
     4501 aataccggag gatgctcgag cagatcgtct ccgacaggaa gcaggggaag agcgtctcct
     4561 acgattcgga cacgcgcagc ttcctcaaga acaagctcga gaccgcccgc tggttcatca
     4621 attccatcca gcagcggcgg cacaccatga tgaaggtgat ggaggcgctc gtgcacctgc
     4681 agcaggattt cttcaagttc ggggagggca acctccggcc catgatcctg aaggacatcg
     4741 ccgacatcat cgggatggac atctcgaccg tgagccgggt cgtgaacggc aagtacgtgc
     4801 agtgcgactt cggcgtctac gagctcaagc atttcttctc ggaggggctc gcgaccgaca
     4861 gcggggagga ggtgtcgaac aaggaggtga aggcgatcat cgaggggatc atcgccgccg
     4921 agaacaaggc gaagccgctc tcggaccagc ggatcgcgga gatgctcgag gagaagggat
     4981 tccagatcgc gcgccggacg gtgaccaagt atcgcgaggc gctcggcgtc cccgtcgcgc
     5041 ggctccggaa ggagatcgtg ctttcgtgag gtttccgggg gccgggatcc cggggcgcgc
     5101 cggatccgtt ttctcctcgc ccagacccgg tacgagccca tgacgtctct cgaagcctgg
     5161 ttcgccccgt tccggcggaa caccatcgga ttcgacgccc gtttcgacac tccgtacggc
     5221 cggcagaagc tggtctacgc cgactggatc gcctccgggc gcctgtatgc cccgatcgag
     5281 agccggatgg cccacacgtt cgggccgttc gtgggcaaca cgcattccga gtcgagcgtc
     5341 acgggcacgt ccatgacgag ggcctaccac gaggcccggc gcatcatcaa gcggcacgtg
     5401 aacgcggggc cggacgacgt catcatcgcg caggagagcg ggatgacggg ggtcgtgaac
     5461 aagctgcagc gcatcctggg cctgcgcgtg cccgagcggc tgctcccgta cgtgtccctc
     5521 ccggagtccg agcggcccgt ggtcttcatc acgcacatgg agcatcactc caaccagacc
     5581 tcctggctcg aaacgctcgc cgaggtcgtg tgcgtggatc cggacgacga cggcctggtg
     5641 gacccggaaa aactccgcga ggccgtctca cgccacgccg gacggccgct catcggttcg
     5701 ttcacggcgt gctcgaacgt gacgggcgtc cggacgccga tccacgagct cgcgcggatc
     5761 atgcacgagg ccggcggcct gtgcttcgcc gactacgcgg cctcggcgcc gtacgatccg
     5821 atcgacatgc atcccgacga cccgctcgag cggctcgacg cgatcttctt ctcgccgcac
     5881 aagttcctgg gcggccccgg gtcggcgggc gtcatggtgt tcgatggccg cctggaccgc
     5941 aaccgcgtgc ccgaccatcc gggcggcggc acggtggact ggacgaatcc gtggggcgag
     6001 catcggttcg tcgagaacct cgaggaccgc gaggacggcg gcacgccggc gttcctgcag
     6061 accatccgcg cggcgctcgc ggtccggctc aaggaggaga tgaaccccga ccggatgcgc
     6121 gagcgcgagg acgagatgct cgcgcgcgcg attcccgccc tgcgcgcggt gccgggcctg
     6181 cacatcctgg ccgaccacct ggaggaccgg ctcggaatcc tctcgttcta cctggaccaa
     6241 gtgcactaca acctggtcgt gaagctgctc aacgaccggt tcggcgtcca ggtccggggc
     6301 ggctgctcgt gtgccgggac ctacggccac tacctgctgc acgtggaccg cgaccggtcg
     6361 cgccgcatca cggaggccat cgaccacggg gacctgtccg agaagcccgg ctgggtgcgt
     6421 ctctcgctgc atccgacgat gaccgaccgg gaactggact ggactctgga cgcgatccgt
     6481 cagatcgccg gtcacgccga cgagtgggcg gccgactacc gctacgatcc gcactcgaac
     6541 gagtacgtgc accgggccga cgtggcgcgg gcggacgcgg tcgcggcctg gttcgagccg
     6601 gccgtcgcgg agcaggaggc gcgggcggcg gtctgagcct cgcggcgccg gaaaagctgt
     6661 ccgccgggcg gggcacggag tgcgcggccg tggacggcgg ggttatgcgg acgcccctcc
     6721 cggcttcaat ggggacaccg gcccccggaa ttcacgcccc cccggattcc tctgtgccgg
     6781 tcccgtttcg ggcgccgccg ggaggctcgc cgtgcgggcg gaacgcggat tcgtgggcgc
     6841 cgcgcgtcgc cggggcattt tggcaagacc tttgcaatag tgcggcaccc ggctccgttg
     6901 aacttccagc ctgcgaggaa ccccatggcc gccgcctccc ttcggaaaac gagcaagtcc
     6961 ctctccgcac tgtttgcccg ctacgtccgc ggagagatcg gcgacccgat ctggtcgcgc
     7021 ctcatgcacc tgctggacgc ggacgacgtg gagcgccccg accgtctcgc cctcgcgcgc
     7081 ttcttcaacg acgtggtcgc cgagggtggg ccgggcgcgc tcttcgtccc gaaggacgac
     7141 gaggcgaggg ccctgctcgt cgacatccga cccgccgttc cgcttcgtcg aggacccctg
     7201 ggcgatcgca ctgcctcctc cgtgtgatgg gccggccggt gcccgtgggt gcaccggacc
     7261 gatgggcttc gacgcggggc cggatgccat gtggcggcgt ccggcccctc ttctttgtgc
     7321 gcaggcacgt cgcccccgct cgcggaccgt ccggcgcggc cctccgcgct caggcgaatc
     7381 ccaccggcgt ctccgcggcc tcctccgccg gcgccgccaa cgcctcgggc cgcgcctccg
     7441 cggccccttc gaagaaggcg acctcgcttc cgatccacgc cacggtcacc ccggcacgcc
     7501 ccagcatcag gacctgctgc agcaggaaca cgacgagcga agcgccgatg gcggcctcca
     7561 ggaccgtggg caggagccag aagaggcccg ccggcacgag ccacgcgagg tacaggagga
     7621 acacgcccca gtggcgcagc gcgaagcgga tcccgtcgac gaacgcggcg aataccccgc
     7681 tgccgcgcag gacgatcgat atccggccca ggtcctggaa caggtccatc ttcgcgagtc
     7741 cgatcacggc gaagagcggc actacgacgg tccacgtccg gaacgtgccc gcctcgcccg
     7801 agagggcgtc caggccgacg cccgcgagca cgatcacgaa gaggacgaac gcgaccggaa
     7861 ccacgtacag gagcgagagc ccgaacgagc ggccgaagta gcggccgacg ccggtccaga
     7921 acgagcccgt gccgtcgtcc ctgagggcgt ggaccaggcc cacgccgagc gcggccttcc
     7981 aggcgaggac gaccgggacg atccagaaca gctgcgccgc caggatgcgg aagaggctgc
     8041 cgtgccgaac gaggatgtcc gtcatgagga cgatgtccag gcgctcggcg aggtcggcgt
     8101 cgaagcccgt gccctccagg gcgcttccga gcgcggcccc gatgggcacg gccaggagaa
     8161 aggccattgc caggttgacc acgaagagaa ggagcgcgag gcccgggcgg gcgcgaagcg
     8221 cgcggattcc ggcggagagc gaagcgacga gcatgggagc gcggcggagg agtcgggtgg
     8281 gagaggagat cggcggccga tggctcggaa gaggggggcg gcggtcgacg gatcagaaga
     8341 gggcctggat cacgtacagc gcctgctgca gtcgcgtcgt gaacttgcgg cgctcctttc
     8401 gcgcgaacgc gttcgacccc acggtcgagc ggcggttgtt caggcgctcg gtctcgagcc
     8461 acaggatgcc gtccggatcg atgtaggctt cctcgatcgg cgaggccttc gtgaaggtga
     8521 actcctccca ttcctcgacc ccgtcccagc gaacgtcctc ggtcgccccg tcctcgaacc
     8581 gcacgcggat cgtcaccgga aagtgtccgt cgtgcacgcg ctgcacgcgg acccggctcg
     8641 cgaacatccc gggcccgtcg ccctcggagg gcgcgggcgt cgacgcgatg gcgtccaccg
     8701 cgtagtccac gaccgccgtc ccgtacacga actggtcgaa gaaccaggcc aggtcctgcc
     8761 cggcgacctc ctcgagggtc gcctgcaggt cgcgcgtggt cgggtggcgg aagcgccagc
     8821 gctcgtagta cgtcctcagg aaccggtgca tggtctccca tcccaggtag ccctcgagcg
     8881 cgttcatgat cgtggcgggc ttggaatagg acgcctttcc gtagtccccg aagcggtact
     8941 tccaggacca ggtgaagagc gccccgcgcg ccgggctgtt ccgcgtgtag gacagccgct
     9001 gcacgtcact gtccgagacc aggaggccgg gcagctccag catcgaaccc ggtccgtagg
     9061 cgtcgtccat gatgcgcgac tccacgtacg agttcatgcc ctcgtccagc cacgcctcct
     9121 cgaactcgtt cgaggcgagg agcccgtaga agtactggtg gccgaactcg tgcacggtca
     9181 ccatctcgac cgggcggaac cactcgggca tgccgtaata cgtgccgcac gtgatgagcg
     9241 tcgggtactc catgccgttg gagcctccga tgccgtcgac gagcgtgagg gtcgtgtacg
     9301 gatactcccc gacccagtcg gtgaagcggt cgagcgcgat cttcgccgcc cggaaaggcc
     9361 ggtcggcctg tgcctcgtgc tccggccgga gcagcaggcg gatcgcgacg tgctcccact
     9421 ggtcctccag gaccaggtag ccctcgtagg ccgtccaggc gaagtcgtgc acgtcgtcgg
     9481 cccggtagcg caggacccgg ttcgggccct cggaccgctc ctccgtgcgg acgccggtcg
     9541 cccccaccac gaacgaatcg ggcaccgtga gcgtcacgtc gtacgttccg aagtccgagt
     9601 agaactcgct gttggcgtgg aactggtgcg tgttccacgc gccccgctcc gcgtcggcgg
     9661 ggacgtagcg ctgcccgggc acctcgtaga ccccgatctt gggaaaccac tgtccgacga
     9721 agaagaaggg ccgcccgtcg tcgtcctcca ggaagcccgt gcgcgccgtg atgcgcggca
     9781 tgatcgactc gaagtcgatg tcgagcgcga tcgtctcgcc cgggcccacg ggacgcggca
     9841 gcgagacggc gatgacggtc tcgtcggacg cgttgccgtc gtcgggctgg atgaaccgga
     9901 tgcggtcggt caggtccacc tcgggacccg ggtaggcgag cgagccggcg aggtcggccg
     9961 gcaggtcgga ggcgatgcgc atgcgctgca ccgtgatgcc tccccagcgg tcgtcctccg
    10021 cggcccggaa tccgcggtgc acgccgcccg actcgacctg gaaggtggag cccggcttga
    10081 aggcgttcag gtacaggtgg aactgcagct cgtccacggg gaccgtgtcc gggttgcgcc
    10141 acgtgagccg ctgccggccg gtcacggcgc gccgggcggg gtcgagcgat acgtccatcg
    10201 tgtacgacac gcgccgctcc gagagcgggc cgtcgtggat cgtggtggcc tgggcgcggg
    10261 ccatgcgggg gcacagcgcg gcgacgagca ggaccgccgt gaagaagcgc aagaacattt
    10321 cgttcgggga cggatagagg aggcgtacag ccgagtgtac gaacgccgtg cgacagggtg
    10381 caccggggaa tccccgcgcc ctgcgctcgg ccaccgcgag ccatgctgcc cgattccgtc
    10441 gaactctgcg aggtgggtcc gcgtgacggc ttccagttcg agtcgaagcc cattccgacc
    10501 gaactgaagg tccgggtcat ccgcgacctg gccgcggccg ggcttcgccg gatccaggcg
    10561 gtctcgttcg tgcacccgga ccgggtgcct cagatggccg atgccgagcg ggtcgccgcc
    10621 gccctgccga ccggcactgc gaccgtcttc agcggactcg ccctgaatgc gcggggcgtc
    10681 cttcgggcgg cggacgccgg ccttcgggcc gtggatctct cgatcgccac caacccggtg
    10741 cacgcccgcg acaacgccgg catgtcggtg gaggagggcg tggacatggc cctggccatg
    10801 atcgaagagg gtcgccgcct cgggctcgcg gtccagctgg gcctgcagac agtgttcggc
    10861 tacgccgccc cgggcgacac gccgccggag ctcgtcgtgg acctcgccgc gcgcattgcc
    10921 gcggccggcg tcgagtcgct ctcgctcgcc gactcgacgg ggcttgcggg tccggccgac
    10981 gtgcgacggg tggtccgcgc cgtccgcggg gccgctccgg gcaccgacct ggtgctccac
    11041 ttgcacgaca cgcgcggcct gggcctcgcg aacgtcgcgg ccgccatcga agagggcgtg
    11101 tcccggttcg acacctctct cggcgggctc ggcggctgcc ccttcatccc gggggcgacc
    11161 ggcaacatcg cgaccgagga cacgctctgg ctcctggagc gcctggggat ccgtacgggg
    11221 atcgaccggg ccgccgtggc cgccgtcacg cgcacggtcg ccgcgcacct ggggcgcgac
    11281 ctgcccgggc gcctctaccg gctgcccgaa gcctaggcct ggtccgcgta cgcctcgagc
    11341 ggcgcacatg agcagacgag gttgcggtcg ccgtacgcgt cgttcacccg ccggaccgac
    11401 ggccagaact tgtgctgccg cacccaggcc gccggccagg ccgccttctc gcgggtgtac
    11461 ggccgctccc acgtgtccgc ggtgaccatc gccgcggtgt gcggagcgtt ggccaggacg
    11521 ttgtccgctg gatcggcggc gcccgagagc acctcctgga tctcgccgtg gatcgcgatc
    11581 atcgcctcgc agaagcggtc gagctcggcg cgcgactcgc tctcggtggg ctccaccatc
    11641 atggtcccga gcacgggcca cgacatggtg ggcgcgtgga agccgtagtc catcaggcgc
    11701 ttggccacgt cgacctcggt gagcggcgaa tcgtgccgga gctcgcgcag gtccaggatg
    11761 aactcgtgtg cgacgcggcc gcgctcgccc gtgtacaggg tgccgaagtg gccctcaaga
    11821 cgggccgcca tgtagttggc gttcaggatc gcggcctggg tggcccgccg gaggcccggc
    11881 tcgccgagca gcgcgatgta ggcccacgag atgaagagca cgcccgccga gccgaagggg
    11941 gccgacgcga ccgggccgat ggcccgctct cccccggtcg caatgaccgg atggccgggc
    12001 aggaacggcg aaagatgggg cgccgcgcag atcgggccga cgcccgggcc gccgccgccg
    12061 tgcgggatgg cgaacgtctt gtgcaggttc aggtggcaca cgtcggcgcc gaagtcgccc
    12121 ggccggcaca ggcccacctg ggcgttcatg ttcgcgccgt ccatgtacac ctgcccgccg
    12181 ctggcgtgca cgatgtcgca gatctcgccg atccgctcct cgaagacccc gtgcgtagac
    12241 gggtacgtga tcatgagggc ggcgagccgg tcggcgttcg cctcggcctt cgagcgcagg
    12301 tcgtccacgt ccacgttgcc ccgctcgtcg caggccacga ccacgacctc catgcccgcc
    12361 atcaccgcgc tggccgggtt cgtgccgtgc gcggacgacg ggatcaggca gacggtccgg
    12421 tgcgcctcgc cccggtcgcg gtggaacgcc cggatcacct gaaggcccgt gtattcgccc
    12481 gccgccccgc tgttgggctg cagcgagacg ccgggcagcc cggtgatctc gccgagccaa
    12541 gcccggaggt cgtccatcag gcggcggtaa ccctgcgcct gctcgacggg cacgaagggg
    12601 tgcagcctgc cgaaccgagc ccaggtgatg gggatgagct cggccgccgc gttcagcttc
    12661 atcgtgcacg atccgagcgg gatcatgctg gtcgtgagcg agaggtcgcg cgacgagagc
    12721 cggtgcatgt accgcatcat ctcggtctcg gagcggtacg cgctgaagac ggagtcggtg
    12781 agatacgggg tggagcgggg catgctgccg ccgtagcccg acggaatcgc ggcggcgatc
    12841 gcggtgacgt ccgcctccgc accgaagagc gcgaggagcc gtccgacgtc cccgggagtg
    12901 gtcgtctcgt cgagcgagat gcccacgccg ccgtcgtcga agtaccggag gttgatccgg
    12961 gcggccgtcg cggccgcgcg caccgtcgcc cggcgcgccg ggtcgaccgg gatgcgcagc
    13021 gtgtcgaaat agtccgcgtg cgcgaccggg agcccggccc gttccaggcc ggccgcaagg
    13081 atccgggcga ggtcatggac ccgcgtcgcg atgccccgga tgccgtccgg gccgtggtag
    13141 acggcgtaca tgcccgccat cacggcgagc aggacctggg cggtgcagat gttcgaggtg
    13201 gccttgtcgc gccggatgtg ctgctcccgc gtctgtaggg ccatgcggag cgcggggttg
    13261 ccgtccgcgt ccaccgaaac gccgatgatg cggccgggaa gctggcgctt gaaggcctcc
    13321 cgcgtcgcga agaaggcggc gtgcgggccg ccgtagccca tcggcacgcc gaagcgctgc
    13381 gaggagccca cgaccacgtc ggccccgaac gatccgggcg cctcgagcag ggcgagcgag
    13441 aggaggtccg cggcgacgac cacgtgcgcg ccggccgcgt gcacgcggtc gcagaacgga
    13501 cggtagtcgc gcacggcgcc gtcggtcgcc gggtactgca ccagggcgcc gaccgtccgc
    13561 ccgtcgggct ggaacgtccc gtagtcgccc gacaccacgc ggatgcccag gggctccgcg
    13621 cgggtctcga cgacggccag cgtctgcgga tggcaggcgt cgtcgacgaa gaacgtgtcc
    13681 cgcgcggcat cgcgcgtggc ccggtgcagc atggtcatgg cctcggccgc ggcggtgccc
    13741 tcgtcgagca gcgacgcgtt ggcgatctcc atgccggtca ggtccatgat cgccgtctgg
    13801 aagacgagca gggcctcgag gcggccctgc gcgatctcgg cctggtaggg cgtgtactgc
    13861 gtgtaccagg ccggattctc caggatgccg cgctggatga ccggcggcgt caccgtcgga
    13921 gagtagccca tcccgatgac ggtgagccac tcctggttca ggtccgccat ggcgcgggca
    13981 tcggcgagca tttcgcgctc gctccgcggc tcgggcaggc gcaggggctg gccgagccgg
    14041 atcgaggcgg gcaccgcctg gtccgtgagc gcctcgagcg agggggctcc gatccgctcg
    14101 agcatggcgg ccacgtcggc ttcggacggg ccgatgtgcc gatccacgaa tcggtcggtg
    14161 gggcgcaggt cgatggccat cgcgatgatc ctcgaagatt cggtccaatg aacgccgccc
    14221 ggacgcggat gtcctgacgg gtcgttcaat tctggatgaa acggtgccgc gtcaggcgcc
    14281 gctgcgcaag tgccggagcg ccgcggccgc ggcacgctgc ccggcgatct ttttgctctt
    14341 gcccgtgccc tcgccgacgg ggtcgtcgtg gatgatgacc tgcacggtga accgccgatc
    14401 gtgccccggc ccctcctcgg aaaccacctc gtagcggggc tgcggccaac cccgctcctg
    14461 ggcgtactcg aggagcaggc ttttgtggtt gtcctcgtgc tgcgccagcg aatcgaggtc
    14521 gagcggctcg agcagccggg acgagacgaa ccggcgcgcc gcgtcggcgc cctggtccag
    14581 gtacacggcg ccgatgagcg cctccatggc gtcggagagg atgttcgtgt tcgagccgcc
    14641 gcgcgcctgc ttcatgttgt ccgagaggat gaccaggtcg ccgagcccga gcgcctccgc
    14701 ccgcgaggcg agggcgcggg cgttcacgag cttggccctg agtcgcgtca ggtagccctc
    14761 gtcctcgtcc ggatagcgca ggtacaggta ctcggccgtg gccaggccca gcaccgcgtc
    14821 cccgaggaac tccagccgtt cgttcgaatc ggcatccccg tccacggcgt cgcggaggac
    14881 ggacctgtgc ttcatggcct gtacgtagag atcgaaccgc tccaccggct cgccggtgat
    14941 ccggacgaag ctatccgggt ccaggccgtg cgtgcggcgt tcgggacgca ggcgggaaag
    15001 cgctcggcgc aggaatcgaa gcatgggcgg atgggtggcg gcgccgcaag atacgcggga
    15061 acggcgtgcg tgcagcccga cccgtccttg cgggagcccc gttttcagga cggggcgtcg
    15121 tattctggcc cgagccgtcg accggaagga ggcactcccc atgaccgtac gatccgtcaa
    15181 cccgtacacc gcatccgaga tcgcccgcta cgccgtcctg gacgaggacg cgctccgggg
    15241 tcggctgcgc tccgcggcgg aggccttcga tcggcaccgg tcgacgacca tggaggagcg
    15301 cggaggccac atgcaccgcg tggccgacgt cctcgaggag caggtggacc gctgggccga
    15361 gctgatgacc gccgagatgg gcaagcccgt cgtgcaggcg cgcgcggaag cgcgcaagtg
    15421 cgcctgggtc tgccgatggt acgcggaccg ggcgcccgcg ctgctcgcgg acgaaccggt
    15481 cgagaccgac gctctccgga gcttcgtggc gttcgagccg ctgggcccgg tgctggccat
    15541 catgccgtgg aatttcccgt tctggcaggt cttccgcttt gccgcgcctg cgatcatggc
    15601 gggcaacgtg gcgctgctca agcacgcctc gaacgtgacc ggctgcgcgc tcgccatcga
    15661 ggagatcttc cgggcgggcg gcttcgaccg ggacgagttc cagaccctgg tcatcccgtc
    15721 gtcggtggtg gaccgggtca tcgaggacgg cgtcgtgcgg gctgcgacgc tcaccgggag
    15781 caacgccgcg ggccgcgccg tggggtgcgc cgcgggtcgg gcgataaagc cgtccgtgct
    15841 ggagctcggc ggctccgacc cgttcgtggt gctcgcggac gcggacgtcg cggcggccgc
    15901 ccgtacggcc gtcgcggccc gcatgcagaa caacggccag agctgcatcg ccgccaagcg
    15961 attcatcgtg gagcggcccg tcctcgaggc cttcacggca gcgttctgcg acggggtgca
    16021 ccggctctcg gtgggggacc cgatggatcc ctcgaccgac gtgggcccgc tcgcccgcgc
    16081 ggacctcgtc gacgaggtcg cggaccaggt ggaccgggcc gtgaagggcg gggcggaagt
    16141 gctcgtgggc ggccggcggg gcgagggccc cggctacttc tacgagccga cggtgctcgg
    16201 cacggtgggc gaggagtcgg tggcgttccg cgaggagatc ttcggcccgg tggcgtcggt
    16261 catcgtggcc gaggacgcag acgacgccct ccgcctggcc aaccggacgc ccttcgggct
    16321 cggcggcgcg gtcttcacgg ccgaccctgc gcggggcgag cgcttcgccc gcggcatgca
    16381 ggtcgggtgc gccttcgtca acgggatggt gaagagcgac ccccggctgc cgttcggggg
    16441 catccgcgag agcggctacg ggcgcgagct cgcgggcttc ggcatccgcg ccttcgtcaa
    16501 cgtgaagtcg atctggatcg ccggggcgcc ggcctgagcg gcggactcac gcgcggtccc
    16561 ggcctccgcg cggacgctca cggccggatc gccgcgtgca cggccgccgt gatcgcggag
    16621 ccgagcgccg aagactcgcc ctggtcctcg atcccctcgg tcatgatcac caggacgtac
    16681 ggagcgc
//
LOCUS       DBNHQZ010000246         7464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1699632, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000246 DBNHQZ010000000
VERSION     DBNHQZ010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1699632"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="AC16YU_06550"
     CDS             <1..140
                     /locus_tag="AC16YU_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR6064919.1"
                     /translation="MVMPGDNTQFKVKLIVPVAMEQGLRFAIREGGRTVGAGVVSKIN
                     D"
     gene            280..588
                     /gene="rpsJ"
                     /locus_tag="AC16YU_06555"
     CDS             280..588
                     /gene="rpsJ"
                     /locus_tag="AC16YU_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR6064920.1"
                     /translation="MASQKIRIKLKSYDHTLIDKSAEKIIKTVRSTGAVVSGPIPLPT
                     RKSIYTVLRSPHVDKKSREQFETRAHKRLIDILSTSSKTVDALMKLELPSGVDVEIKV
                     "
     gene            606..1076
                     /locus_tag="AC16YU_06560"
     CDS             606..1076
                     /locus_tag="AC16YU_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064921.1"
                     /translation="MRKLSSLLLIALLALVGIGCDSDDDEGSDAELFVGTWALTGVSD
                     DEGDAMEAFATNFESIVLENDADGSFTLTVNPVVGDNVVLPGTYTVSESNSRLTLTAT
                     VPGLGTANLLFTYEFNGDDEIDLTTVAPTAVLLNSLFDGVDLVGEAVITVSRVD"
     gene            1135..1764
                     /gene="rplC"
                     /locus_tag="AC16YU_06565"
     CDS             1135..1764
                     /gene="rplC"
                     /locus_tag="AC16YU_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR6064922.1"
                     /translation="MSSGMIGRKLGMTSVFDGAGNQIACTVIEAGPNVVTEVRTEDRH
                     GYSAVQLGYGEKKEKRTPKALRGHFERASASPKQVVREFRDFEAEVSLGDEVRLEDVF
                     EEGERIDVAGTSKGKGFQGVVKRHGFSGVGMATHGQHNRQRAPGSIGASSWPSRVVKG
                     MRMAGQTGNARVTMKNLRVVRILADQNLLLVEGAVPGARKGFVELRKKK"
     gene            1779..2426
                     /gene="rplD"
                     /locus_tag="AC16YU_06570"
     CDS             1779..2426
                     /gene="rplD"
                     /locus_tag="AC16YU_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR6064923.1"
                     /translation="MQLKVYSHDGSESGKKVKLDAGVFGIEPNDHAIWLDVRRIQAHD
                     RQGTHKARERSETAGSTRKLYRQKGTGHARSGDAKSPVRRSGGTIFGPRPHEYRLKVN
                     RKTQLLARRSALSHKAKADAIRVIEDLSFGAASAREMRTLLDALQVSGRSVLVLTGAH
                     DPIAYRSGRNLPRVSVRAASSASTLDLMNAQVVILARSAVDVLTGQLGVAATETA"
     gene            2458..2757
                     /gene="rplW"
                     /locus_tag="AC16YU_06575"
     CDS             2458..2757
                     /gene="rplW"
                     /locus_tag="AC16YU_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR6064924.1"
                     /translation="MKSDILIKPLVTEKMTALMEEGHYAFQVRRDANKVQIRKAVETR
                     YPGVQVKEVRTMVVRGKQRSQMTRRGRVAGRTAGYKKAIITLRPESQRIDFFEQI"
     gene            2804..3631
                     /gene="rplB"
                     /locus_tag="AC16YU_06580"
     CDS             2804..3631
                     /gene="rplB"
                     /locus_tag="AC16YU_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR6064925.1"
                     /translation="MAIKQLKPTTPGQRQMSVSAFDTITKSTPEKSLLAPLTRKGGRN
                     NNGRITVRHQGGGHKRRYRIIDFKRDKVGIPAKVASIEYDPNRSARIALLVYADGEKR
                     YIIAPNEITVGMSLLNGPDAPPEPGNCLPLTRVPVGSFVHAIEMRPGKGAQLARSAGT
                     YAQLTAREGGYAILRLPSGETRRVPVGCKATVGTTSNPDHMNIDLGKAGRSRWLGIRP
                     KTRGVAMNPIDHPMGGGEGKASGGHPRSPSGVPAKGYKTRRKKKLSNKYIVRRRSKR"
     gene            3661..3945
                     /gene="rpsS"
                     /locus_tag="AC16YU_06585"
     CDS             3661..3945
                     /gene="rpsS"
                     /locus_tag="AC16YU_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR6064926.1"
                     /translation="MARSLKKGPFVHFKLQRKVDELNRSGKKKVVKTWSRASMITPEF
                     VGHTFAVHNGKQFIPVYVTEMMVGHRLGEFAPTRSFRGHAGSKKDKRVKK"
     gene            4000..4371
                     /gene="rplV"
                     /locus_tag="AC16YU_06590"
     CDS             4000..4371
                     /gene="rplV"
                     /locus_tag="AC16YU_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR6064927.1"
                     /translation="MEARAVRKHIRSSAKKMRPVVNVVRGKRVPEALNSLNFLPQKVT
                     RIVKLTIMSAVHNLMDRNREERLDENELVVREIRVDEGPRFKRFQPVSRGRAHPILKR
                     TAHLTVVIGTAEEEAKASAEA"
     gene            4421..5185
                     /gene="rpsC"
                     /locus_tag="AC16YU_06595"
     CDS             4421..5185
                     /gene="rpsC"
                     /locus_tag="AC16YU_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR6064928.1"
                     /translation="MGQKTHPIGFRLGIIRGWSSNWYAEKDFAEKLVEDDEIRQYLNA
                     RLKRAGLSRVVIERTPKRVILTLHTSRPGVVIGRGGAEVEKLREEIRKLTAKDIQINI
                     NEIKRPELDASLVAQNIAQQLEGRVSFRRAMKQSIGAAMRMGAEGIRIKLGGRLGGAE
                     MGRTERYHEGRVPLHTIRADIDYAEATAFTIYGTSGVKVWIYQGEILGRPDLSPNVQA
                     QRQQLQQMEPERRKGERDRKRRRRATGARTDKKGSN"
     gene            5239..5664
                     /gene="rplP"
                     /locus_tag="AC16YU_06600"
     CDS             5239..5664
                     /gene="rplP"
                     /locus_tag="AC16YU_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR6064929.1"
                     /translation="MLMPKRVKRRRVQKGRIKGNAQRGTRIDFGDFGIKALEIGRITS
                     RQIEAARIAMTRRMKRTGKVWIRIFPDKPITKKPAETRMGKGKGSPEYFVAVVRPGRI
                     LFEVGGGVPKDLAEEAMALAQQKLPIKTKLVTRPDFTSH"
     gene            5669..5860
                     /gene="rpmC"
                     /locus_tag="AC16YU_06605"
     CDS             5669..5860
                     /gene="rpmC"
                     /locus_tag="AC16YU_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR6064930.1"
                     /translation="MKAKEIRDLSVEEITQRIKEEKEQLEHLAFQHAVAELPNPVLLR
                     EKKRLLARLKTILNETTRA"
     gene            5923..6189
                     /gene="rpsQ"
                     /locus_tag="AC16YU_06610"
     CDS             5923..6189
                     /gene="rpsQ"
                     /locus_tag="AC16YU_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR6064931.1"
                     /translation="MEQTQARKSRKERVGIVVSSRMNKSILVAIQRQIKHPIYGKFIK
                     KTTKLMAHDENNDAGEGDTVRIVETRPLSKNKRWRLVEIVERAK"
     gene            6246..6614
                     /gene="rplN"
                     /locus_tag="AC16YU_06615"
     CDS             6246..6614
                     /gene="rplN"
                     /locus_tag="AC16YU_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR6064932.1"
                     /translation="MIQQETRLKVADNSGAKEVLCIRVLGGSGRRYARVGDTIVVSVK
                     SAIPGGNIKKGEVSRAVVVRTTKEYRRKDGSYIRFDENAAVLLNAQGEPRGTRIFGPV
                     ARELRERQFMRIVSLAPEVL"
     gene            6657..7013
                     /gene="rplX"
                     /locus_tag="AC16YU_06620"
     CDS             6657..7013
                     /gene="rplX"
                     /locus_tag="AC16YU_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR6064933.1"
                     /translation="MPRTTKSTHKLHVKKGDMVRVVAGNDNGKEGKVLRVFPETQRVI
                     VEGVNLRVRHTRPNKTYPQGGRIQQERGIHVSNVMPLDSSGDPTRIGRKRIEDAETGR
                     GRWIRFAKTTGEELDR"
     gene            7068..>7464
                     /gene="rplE"
                     /locus_tag="AC16YU_06625"
     CDS             7068..>7464
                     /gene="rplE"
                     /locus_tag="AC16YU_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR6064934.1"
                     /translation="MEGYVPRLKTKYQEVVAPALTKEFGYTNSMQLPRLAKISVNKGV
                     GEASANKKVLDDAVDELRRITGQQPHVRKARKSVSNFKLREGMPVGVAVTLRGDRMYE
                     FLDRLITLALPRVRDFRGVPDRSFDGRGNY"
BASE COUNT         1548 a         2440 c         2475 g         1001 t
ORIGIN      
        1 agatggtgat gccgggcgac aacacgcagt tcaaggtgaa gctgatcgtg ccggtggcga
       61 tggagcaggg gctgcggttc gccatccgcg agggtggccg gacggtcggc gccggcgtcg
      121 tgtccaaaat caacgactga ggtcttcgat gaatccgctc actcttcgtt ggacgtcgtc
      181 gcccgtcgct gcgacgcacc tcggtgcgtc tcgctccggc tcctcgtccg cctcgattga
      241 tcgaattcct ctttgacctc cagcaacagg cgagcaccga tggccagcca gaagattcgc
      301 atcaagctca agtcgtacga tcacacgctg atcgacaaga gcgcggagaa gatcatcaag
      361 acggtccggt cgaccggcgc ggtggtgagc ggtcccattc cgctgcccac gcgcaagtcg
      421 atctacacgg tgcttcgcag cccccacgtg gacaagaaga gccgtgagca gttcgagacg
      481 cgcgcccaca agcgcctgat cgacatcctg tccacctcca gcaagacggt cgacgccctg
      541 atgaagctcg agcttccgag cggcgtggac gtcgaaatca aggtctgacc caaacggaga
      601 aacccatgcg caagctttcc agcctgttac tcatcgccct gctcgccctg gtgggcatcg
      661 gttgcgactc cgacgacgac gaaggatccg acgccgagct cttcgtcggc acgtgggcgc
      721 tcacgggcgt ctcggacgac gagggcgacg cgatggaggc gttcgccacg aacttcgaga
      781 gcatcgtgct cgagaacgac gccgacggct ccttcacgct cacggtgaac ccggtcgtcg
      841 gcgacaacgt cgtgcttccc ggcacctaca cggtgtccga gagcaactcg cggctcacgc
      901 tgacggccac cgtgccgggg ctgggcacgg cgaatctcct gttcacctac gagttcaacg
      961 gggacgacga gatcgatctg acgaccgtgg ccccgacggc cgtgctgctt aacagcctgt
     1021 tcgacggcgt cgacctggtc ggagaggccg tgatcacggt ttcccgcgta gactgaacgt
     1081 agcactcgac cgcttaacaa tccggccgcg gagtcggcca gaaccgggat tgcaatgagc
     1141 tcaggaatga tcggcaggaa actgggaatg accagcgtct tcgacggcgc cggcaaccag
     1201 atcgcctgca ccgtaatcga ggccgggccc aacgtcgtga ccgaggtcag gacggaggac
     1261 aggcacggct acagcgccgt gcagctcggc tacggcgaga agaaggagaa gcgtaccccg
     1321 aaggcgctcc gcggccactt cgaacgcgct tccgcctcgc cgaaacaggt ggtccgggag
     1381 ttccgcgact tcgaggccga ggtttccctc ggcgacgagg tccggctcga ggacgtgttc
     1441 gaggagggcg agcgcatcga cgttgccggg acctcgaagg gcaagggttt ccagggcgtg
     1501 gtgaagcgcc acgggttcag cggcgtgggc atggcgaccc acggtcagca caaccgccag
     1561 cgggcccccg gctcgatcgg cgcgtcgtcc tggccgtccc gcgtggtcaa gggcatgcgc
     1621 atggcgggcc agaccggcaa tgcgcgcgtg accatgaaga acctgcgggt ggtgcggatc
     1681 ctggccgacc agaatctcct tctcgtggag ggggcggttc cgggagcccg gaagggattc
     1741 gtggaactgc gcaagaagaa gtgagcggag gcgaaaccat gcaattgaag gtatacagcc
     1801 acgacggatc cgagtccggg aagaaggtca agctcgacgc cggcgtgttc ggcatcgagc
     1861 ccaacgacca cgcgatctgg ctcgacgtcc ggcgcatcca ggcccacgac cgccagggca
     1921 ctcacaaggc ccgggagcgg agcgagacgg cggggagcac ccgcaagctc tatcgccaga
     1981 agggcacggg ccacgcgcgc tcgggcgacg cgaagtcacc cgtacggcgg agcggcggca
     2041 cgatcttcgg accgcgtcct cacgagtacc gcctcaaggt gaaccggaag acgcagctcc
     2101 tggcccggcg ctcggcgctc tcgcacaagg ccaaggccga cgcgatccgc gtgatcgagg
     2161 atctctcgtt cggggcagcc tccgcgcgcg agatgcgaac gctcctcgat gcgctgcagg
     2221 tctcgggccg ttcggtcctg gtgctcacgg gcgcccatga cccgatcgcg taccggtcag
     2281 gccgcaacct gccgcgggtc tccgtgcgtg cggcgtccag cgcttccacg ctcgacctca
     2341 tgaacgccca ggtggtgatc ctggcccgct cggccgtgga cgtactcacg ggccagctgg
     2401 gcgtggccgc cacggaaacg gcctgatccg caaccgccac cggagacatc cacagcgatg
     2461 aaaagcgaca tcctcataaa gcctctcgtc accgagaaga tgacggcgct catggaagag
     2521 ggtcactacg ccttccaggt gcgccgcgac gccaacaagg tccagatccg caaggcggtc
     2581 gagacccggt accccggtgt ccaggtgaag gaggtccgga ccatggtggt ccggggaaag
     2641 cagcgcagcc agatgacgcg tcgcggccgg gtggccgggc gtacggccgg ctacaagaag
     2701 gccatcatca cgctgcggcc ggaaagccag cggatcgact tcttcgagca gatctgaacc
     2761 cgctacccgc cgaagcgcga taacgcagag acattccaac gtcatggcga tcaaacagtt
     2821 aaagccgacc acgcccgggc agcgacagat gtcggtctcg gcattcgaca cgatcacgaa
     2881 gtcgacgccc gagaagagcc tgctcgcgcc cctgaccagg aagggcggcc gcaacaacaa
     2941 cggccggatc accgtccggc accagggggg cggccacaag cggcgctacc ggatcatcga
     3001 cttcaagcgc gacaaggtcg gcattccggc caaggtggcc tcgatcgagt acgacccgaa
     3061 ccgctcggcc cgaatcgcgc tcctggtgta cgccgacggc gagaagcgat acatcatcgc
     3121 gccgaacgaa atcaccgtcg gcatgtcgct cctgaacggg ccggacgctc cgccggagcc
     3181 cggcaactgc ctcccgctga cccgggtgcc ggtcggctcg ttcgtgcatg ccatcgagat
     3241 gcggcccggc aagggcgccc agctggcgcg ctcggcgggc acgtacgccc agctcacggc
     3301 gcgggagggc ggctacgcca tcctccgcct gccgtcgggc gagacccggc gcgtgccggt
     3361 cggatgcaag gcgaccgtcg gcacgaccag caacccggat cacatgaaca tcgacctggg
     3421 caaggccggt cgcagccgct ggctcgggat ccgtcccaag acgcgcggcg tcgcgatgaa
     3481 cccgatcgac caccccatgg gcggcggtga gggcaaggcc tccggtggac acccccgctc
     3541 cccgagcggc gtccccgcca agggctacaa gacccgcagg aagaagaagc tttcgaacaa
     3601 gtacatcgtg cgtcgccgca gcaagcggta ggcacctagc gcagaaacac tggatcagcc
     3661 atggcgcgat cgctgaaaaa aggccctttc gttcacttca agctccagcg gaaggtggac
     3721 gagctcaacc gctcgggcaa gaagaaggtc gtcaagacct ggagccgggc ctcgatgatc
     3781 actcccgagt tcgtcgggca cacgttcgcc gtgcacaacg gcaagcagtt catcccggtc
     3841 tacgtgaccg agatgatggt gggccaccgg ctcggcgagt tcgcccccac ccggagcttc
     3901 cgcggccacg cgggctccaa gaaggacaag cgcgtaaaga agtagtcggt cgcccgtccg
     3961 ggcggacccg atcggcaacc gacacgacac gaaccgaaca tggaagcgag agcagtcagg
     4021 aagcacatcc gcagctcggc caagaagatg aggccggtgg tcaacgtggt gcgcggcaag
     4081 cgcgtgcccg aggccctgaa ttccctcaac ttcctgccgc agaaggtcac gcgcatcgtg
     4141 aagctgacca tcatgtcggc ggtccacaac ctgatggacc gcaacaggga ggagcggctg
     4201 gacgagaacg agctcgtcgt gcgcgaaatc cgggtggacg aggggccgcg attcaagcgg
     4261 ttccagccgg tctccagggg gagggcccac ccgatcctga agcggacggc gcatctcacg
     4321 gtcgtcatcg ggacggccga ggaagaagcg aaggcaagcg cagaagcctg acgaaacccg
     4381 ggaaaccaca aggccggccc agccggcgag taccgtacac atgggtcaga aaacacaccc
     4441 gataggattt cgcctcggca tcatccgcgg ctggagctcc aactggtacg cggagaagga
     4501 cttcgcggag aagctcgtgg aggacgacga gatccggcag tacctgaacg cccgcctcaa
     4561 gcgggcgggc ctgagccgcg tcgtcatcga gcgcacgccc aagcgtgtga tcctgacgct
     4621 gcacaccagc cgcccaggcg tggtgatcgg gcgaggtggc gcggaggtcg agaagctccg
     4681 cgaggagatc aggaagctga ccgccaagga catccagatc aacatcaacg agatcaagcg
     4741 tcccgagctc gacgcctcgc tcgtcgcgca gaacatcgcg cagcagctcg agggccgcgt
     4801 gtcgttccgc cgcgccatga agcagtcgat cggagcggcg atgcggatgg gggccgaggg
     4861 catccgcatc aagctgggcg gccgcctggg cggcgccgag atgggacgca ccgagcggta
     4921 ccacgagggg cgcgtgccgc tgcacacgat ccgggcggac atcgactacg cggaggccac
     4981 ggccttcacg atctacggca cgtccggcgt caaggtctgg atctaccagg gcgagatcct
     5041 cggccggccc gacctgagcc cgaacgtgca ggcgcagcgc cagcagctgc agcagatgga
     5101 gccggagcgc cgcaagggcg agcgcgaccg caagcgtcgc cgccgcgcca cgggcgcccg
     5161 caccgacaag aagggatcca actgaccaga gaacttgccg ggcgtcccac ggacgaccga
     5221 tagaagaggg aatcgaccat gctcatgccg aagcgcgtca aacgacgccg agtacagaag
     5281 gggaggatca agggcaacgc ccagcgcggg acccggatcg acttcggcga ctttggcatc
     5341 aaggcgctgg agatcggccg gatcacgagc cggcagatcg aggcggcccg tatcgccatg
     5401 acgcgccgga tgaagcgtac cgggaaggtg tggatccgca ttttccccga caagccgatc
     5461 acgaagaagc ccgccgaaac ccgaatgggc aagggcaagg gctcgcccga gtacttcgtg
     5521 gcggtcgtcc gccccggccg catcctgttc gaggtcggcg gcggggtgcc caaggacctg
     5581 gccgaggagg cgatggccct ggcgcagcag aagctcccca tcaagacgaa gctggtcacc
     5641 cggccggact tcacctccca ttgacatcat gaaggcaaag gaaatccgcg atctcagcgt
     5701 cgaggagatc acgcagcgca tcaaggagga gaaggagcag ctggagcacc tcgcgttcca
     5761 gcatgccgtc gccgagttgc ccaacccggt gctcctccgc gagaagaagc ggctgctcgc
     5821 acgcctgaag accatcctga acgaaaccac ccgggcctga ccccgatcga acggtagttg
     5881 gtgaggacac ggtacacggc tcaccaagcc acacacagca ccatggagca gacgcaagca
     5941 agaaagtcac gcaaggagcg cgtcgggatc gtcgtctcga gccggatgaa caagagcatt
     6001 ctcgtggcca tccagcggca gatcaagcat cccatctacg ggaagttcat caagaagacg
     6061 acgaagctga tggctcacga cgagaacaac gacgccggcg agggcgacac ggtccgcatc
     6121 gtggagaccc gtccgctcag caagaacaag cgctggcgcc tcgtcgaaat cgtagagcgg
     6181 gccaagtagc gccgccggcc ggctcgccgg tcggattcac ccggactgac agcacgaaca
     6241 tcgtcatgat ccagcaggag acgagactca aggttgccga caacagtggc gcgaaagagg
     6301 tgctctgcat tcgcgtgctc ggcggaagcg ggaggcggta tgcccgcgtg ggcgacacga
     6361 tcgtcgtgtc ggtgaagtcc gccattcccg gcggaaacat caagaagggc gaggtttcgc
     6421 gggcggtggt cgtgcggacc acgaaggagt accggcgcaa ggacgggtcc tacatccgct
     6481 tcgacgagaa tgccgcggtg ctgctcaacg cccagggcga gccgcgcgga acccgcatct
     6541 tcggtcccgt ggcgcgtgag ctccgcgagc gccagttcat gagaatcgtg tcgctggccc
     6601 ccgaggtcct ctgaccccac gcatgcttcc aggcggcgat caacccgagc agaacgatgc
     6661 ctcgcacaac gaaatcaacg cataagcttc acgtcaagaa gggcgacatg gtccgcgtgg
     6721 tcgccgggaa cgacaacggc aaggagggca aggtcctccg cgtgttcccg gagacgcagc
     6781 gcgtgatcgt ggaaggcgtg aacctccgtg tccggcacac gcgcccgaat aagacctatc
     6841 cgcagggtgg acgcatccag caggagcgcg ggattcacgt ctcgaacgtg atgcccctcg
     6901 actcctcggg cgatccgacc cgaatcggcc gcaagcggat cgaggacgcg gagaccggcc
     6961 gcggccgctg gatccgcttc gccaagacca cgggcgaaga gctcgaccgg tagtcaacgc
     7021 gcgcagacat gcaccgccgg caagtccggc cgaatccgac agcaacgatg gaaggctacg
     7081 tacccaggct caagaccaag taccaggagg tcgtcgcacc cgcgctcacg aaggagttcg
     7141 ggtacacgaa ctccatgcag ctgccgcgcc tggccaagat cagcgtcaac aagggcgtgg
     7201 gcgaggcgtc ggccaacaag aaggtccttg acgacgccgt ggacgagctg cgccgcatca
     7261 ccggccagca gccgcacgtc cggaaggccc gcaagagcgt gtccaatttc aagctcaggg
     7321 aaggcatgcc cgtcggggtg gcggtgacgc tccgcggcga ccgcatgtac gagttcctgg
     7381 accggctgat cacgctcgcc ctcccgcgcg tccgcgactt ccgcggggtg cctgaccgat
     7441 ccttcgacgg ccggggcaac tact
//
LOCUS       DBNHQZ010000247        10231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3052750, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000247 DBNHQZ010000000
VERSION     DBNHQZ010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3052750"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1462)
                     /locus_tag="AC16YU_06630"
     CDS             complement(11..1462)
                     /locus_tag="AC16YU_06630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004348 - glucosylceramidase activity
                     [Evidence IEA];
                     GO_process: GO:0006665 - sphingolipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HIR6064935.1"
                     /translation="MPRYRPSTSRRPAIGPWVILVLMASLGLGPGCDQGNADPDGAPG
                     PELWLSDPARNVRFQKQVAGPAEGAANTSQGILVDPSVAYQEMDGFGFALTGGSALHI
                     HNMSAPARADLLDELFSTAGNAIGTSYLRVSIGASDLDEEVFSYVDLPSGQTDPDLEH
                     FTLDRDREHLIPVLKEILAINPDIRILGSPWSPPVWMKTNGSSIGGSLLPEYYESYAQ
                     YFVKYVQGMAAEGITIDAITVQNEPLHPGNNPSMLMQADEQAAFVRDALGPAFQAAGI
                     ATKIIIYDHNADRPDYPIAVLNDQAARVYIDGSAFHLYGGTIDALSQVHAAHPDKNLY
                     FTEQWIGAPGNFAADLKWHVRELIVGATRNWCRAVIEWNLAADQNQDPHTPGGCTQCL
                     GAVTVQGDVVTRNPAHTIIAHASKFVRPGSRRIASTSTTELPNVAFSTPSGDVVVIVL
                     NNGTTPVTFNLVIGDKTWHSSLPAGAVGTYVVE"
     gene            complement(1396..1935)
                     /locus_tag="AC16YU_06635"
     CDS             complement(1396..1935)
                     /locus_tag="AC16YU_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064936.1"
                     /translation="MRYLLALALVFAARDQTPLEVPSRERPTIDGRWVSTWVDADEGE
                     YNTRYVLDITEAASGRCAAMLSRQCSSEDHIPENPGNRFREGERWNGSLPMTSTNLVT
                     GETANLTASLASGTRGNSWFRNGRPHDIPAVTFAVHRGSSGHAEAAARPPPFRRGPCR
                     VTVPPRPAAPRSAPGSFSC"
     gene            complement(2042..2416)
                     /locus_tag="AC16YU_06640"
     CDS             complement(2042..2416)
                     /locus_tag="AC16YU_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064937.1"
                     /translation="MGTLRRYRRPLGVVFLVTLLDLAARVGFGFDMGRVVAVEASLFL
                     AAGVLLGWMGASPDASDPGVRRLDLWLAAGFLLAGLRAALWTAGLGVHVANLAVLLVA
                     AAMGIALWIVRQRRSRPKARGD"
     gene            2540..5581
                     /locus_tag="AC16YU_06645"
     CDS             2540..5581
                     /locus_tag="AC16YU_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6064938.1"
                     /translation="MRRTILLLAALALAPGAFAQTIDPALFAGLEYREVGPYRGGRSA
                     AVTGVPGRPMLYYMGATGGGVWRTEDGGGSWESVSDGYFGGSIGAVAVSEWDPNVIYV
                     GGGEKTVRGNVSHGDGVWKSVDAGKTWTHVGLADTRQVPRIRIHPRNPDLVYVAALGH
                     LYGPNDERGVFRSRDGGATWERILFVSDGAGAVDLVMDPTNPRILYAATWKVFRTPWL
                     LSSGGEGSALWKSTDGGDSWTELTGKGNGLPEGTLGIIGVAVSPVRPERVWAIVEAED
                     GGVFRSEDGGGTWRRVNDDRNLRQRAWYYTRIYAGTGDVDDVWVLNVALWHSTDGGAT
                     YEQVRTPHGDHHDLWIAPEDADRLVVGDDGGAQVSLDGGASWSTMYNQPTAQFYRVTT
                     DNHFPYRIYGGQQDNSTVRILHTSDGRSEREWEPTAGGESAWIAVDPTNPDLAYGGSY
                     GGYLERVDHATGQSRNVNAWPDNPMGHGAEDYRFRFQWNFPIRISRHDPSVVLTGANV
                     LFRSEDGGQSWTAISPDLTRAEPSTLGPSGGPITKDNTSVEYYGTIFTFAEGEEPGVI
                     WTGSDDGLIHLTRDGGATWTDVTPPARVFPEWMQVNDMVPDPHNPAGLYVAGTRYRSD
                     DFSPYLYRTADYGATWTRIDQGIDRMHFTRTIEPDPVRAGLLYAGTENGMVISFDDGA
                     SWSPFQLNLPQVPITDLEWKDHDLVVATQGRGFWILEDLTLLHRLQPADAERDFVFYG
                     PHAATLGAESAVRLRFWFAAAPDSEAVVRLRILEDDGDVVRTFSSGGPDGESLEPKLG
                     VNTFEWNMRYPDAESFDGLVMWSGSVRGPRAAPGAYRARLVVGDDSTEVPFEIRPDPR
                     SASTEADLRGQFEFLLGIRDKLSEIHRAIREIRAIREQTAGVLERVPADHPDRDTLRA
                     AADAMNAALRSVEEALYQVKNRSGQDPLNFPIRLNDKLAGVASTAATGHYRPTDQAVA
                     VRDELVARIDAELDRMRAVLEADLSAFNALVRAADIPAVAR"
     gene            5578..6834
                     /locus_tag="AC16YU_06650"
     CDS             5578..6834
                     /locus_tag="AC16YU_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064939.1"
                     /translation="MTTSRMMRATDRTLKAAVALFVLAGLTGAVFRMAGAYGWTLGFN
                     PVNIRHAHSHLMYFGWAIPALFALVAARVVRSGGRPPSCRLLSSSFIVAVAAWESFLL
                     WGYESARIGGARIPVSIIVSTLAMLLWYWFAHWYRRGREHLEAGSARTLLDVSVVLLV
                     VSTFGAWGVAATVPAGESLFALKTAFTHLFLALFSEGWLVTALLGLAWAELPSGARMP
                     RWALPLLLAGTAASFLLGMPGHLVGENLRLVARLGGMAWGVGAVGIVWGWWRAATAPL
                     RASWGFPMLFLALKGSVQAASMLVPGLWWADIPAMRVLYLHVLLLGFVTLAIAASLVR
                     GPRLAAVQAAALLVILSLAPMTDLWPGFLSLDRPTEVAAWVSLVLPVAAMLLWRRAYF
                     GGREVPGLGLRDGAPGRSLPAPGDAA"
     gene            complement(6831..7958)
                     /locus_tag="AC16YU_06655"
     CDS             complement(6831..7958)
                     /locus_tag="AC16YU_06655"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6064940.1"
                     /translation="MAKRLRVGLLTGGGDAPGLNAVIRAVTKSLILKHDAEVVGFEDG
                     FLGLIAQRIKPLSYGDVSGILTRGGTILGTNNKANPFRYYKRGGADVSSQVIAYAKQL
                     GLDGIVAIGGDGTMSIANRLNQMGLPMVGVPKTIDNDLVATDRTFGFDTAVSIVTEAV
                     DRLHTTAQSHQRVMIVETMGRYAGWIALHAGVAGGADLILIPEFEFSVDEVARVCRDR
                     ESFGRSFTIIVIAEGARITGGEMAVQRTLADSPDPIRLGGIGYVLQAALEAEIKSEVR
                     TTILGHVQRGGTPTAYDRNLATSFGAIATDLVAAGEFGRMVAVQNGQFTSVPLDEVAD
                     RVRKVPLDAPLIRAALGVGTSFGVPELSVRLDGARGGAAVI"
     gene            8140..9852
                     /locus_tag="AC16YU_06660"
     CDS             8140..9852
                     /locus_tag="AC16YU_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR6064941.1"
                     /translation="MSYCVPGIRISTWAWLRIACFVALAHPANGQSLRVSAPNRASAG
                     REVAFYVGLEEGRRFDGAVLAMPMAWSLVRAVVVGGDGGTMDLEPWTDERAPGRTRLD
                     PTGSVREPTELRLHVRLGREPMDGRWSLVPLERAAGWDDVGFVVRESGRVTRTLRVDE
                     PQSSGENRVLRIGDGGPPVPLDGRRLPDLGTSKAWTLETWFRTVALDAVLVSTWDGDE
                     ATDYPAELVIDPVGRLVFFQGLGGRHRSLRGPQAVADGGWHHVAVTHDPDRGWTRLLV
                     DGAAVDSLHGFDMHGIARPGRVVLGSRTARRPDDGILDFAGEMDEFRIWAASRTARNV
                     RDGMSRPLAQPPGGALILGFDRGIEPGLVAGDGVRALIVRSDLTFRRPVRDLAVRMTA
                     GYVELSWSGDEGTGTIYHVERSADGALFEPVARYTNAQGSPGAVSGSRSFRHGEHATE
                     GRVAWYRIRQVEATGAENLTPVVKVGVGSAHAVSRATLEGAWPNPFNPNTTIGYRVDR
                     GGEVAVSVWDLSGQRVRVLADAYHEPGQYTVSFAAEELPSGAYFVRLETVDGIQIRQI
                     VLAK"
     gene            9929..>10231
                     /locus_tag="AC16YU_06665"
     CDS             9929..>10231
                     /locus_tag="AC16YU_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6064942.1"
                     /translation="MNLTYFGHSAFRIRVDGATLLVDPFISGNPLAEPVVTAAELSAD
                     VILLTHAHGDHWGDTASIAKRTGALVVANFEITEVLRKKHGHANVHAMNTGGSVDFP"
BASE COUNT         1446 a         3505 c         3661 g         1619 t
ORIGIN      
        1 ttcggcgctc tcactccacg acgtacgtcc ccaccgctcc cgccggaagg ctggaatgcc
       61 aggtcttgtc gccgatcacg aggttgaacg tcacgggcgt ggtaccgttg ttgaggacga
      121 tcacgacgac atcaccagat ggcgtgctga acgccacgtt ggggagctcg gtcgtcgatg
      181 tcgaggcgat ccggcgcgag cccggacgca cgaacttcga ggcgtgcgca atgatggtat
      241 gggccgggtt ccgcgtgacc acgtcgccct ggaccgtgac ggccccgaga cactgcgtgc
      301 agccgccggg cgtatgcggg tcctggttct ggtcggcggc caggttccac tcgatgaccg
      361 cgcggcacca gttgcgcgtc gcgcccacga tgagctcgcg cacgtgccac ttcaggtccg
      421 ccgcgaagtt gcccggtgcg ccgatccact gctcggtgaa gtacaggttc ttgtcgggat
      481 gggcggcgtg cacctggctc agggcatcga tcgtgccgcc gtacaggtgg aaggccgagc
      541 cgtcgatgta gactcgcgcc gcctggtcgt tcagcaccgc gatgggatag tccgggcggt
      601 cggcgttgtg atcgtagatg atgatcttcg tggcgatgcc cgccgcctgg aaggccgggc
      661 ccagggcgtc cctgacgaac gccgcctgct cgtcggcctg catgagcatg ctcgggttgt
      721 tgccggggtg cagcggctcg ttctggacgg ttatggcgtc gatcgtgatg ccctcggccg
      781 ccatcccctg gacgtacttc acgaagtact gcgcgtaaga ctcgtaatac tccggcagca
      841 ggcttccgcc gatcgaactg ccgttcgtct tcatccagac gggcggcgac cagggcgatc
      901 ccaggatcct gatgtcgggg tttatcgcga ggatctcctt caggacgggg atcaggtgct
      961 cgcggtcccg gtccagggtg aaatgctcga gatccgggtc cgtctggccg ctcggcaggt
     1021 cgacgtacga gaagacctcc tcgtcgaggt cggacgcgcc gatgctcacg cggagatagc
     1081 tcgtcccgat ggcgttgccc gccgtcgaga acagctcgtc gagcaggtcc gccctcgcgg
     1141 gcgccgacat gttgtggatg tgcagggcgc tgccgcccgt cagcgcgaag ccgaatccgt
     1201 ccatttcctg gtacgcgacg gacgggtcga ccaggatgcc ctgcgacgtg ttcgccgcgc
     1261 cctcggcggg gcccgcgacc tgcttctgga agcggacgtt gcgggcgggg tcgctcagcc
     1321 agagttccgg tccgggagcc ccgtccggat ctgcgttccc ctggtcgcag ccgggaccga
     1381 ggccgagcga ggccattagc acgagaatga cccaggggcc gatcgcgggg cggcgggacg
     1441 tggagggacg gtaacgcggc atggtcctct gcgaaagggg ggcggacgtg cggcggcctc
     1501 ggcgtggccg ctcgatccac ggtgaacggc aaacgtcact gcaggaatat cgtgaggccg
     1561 cccgttacga aaccacgaat tcccccgcgt gcccgacgcg agcgatgcgg tcaggttggc
     1621 cgtctcgccc gttacgaggt tggtggaggt catgggcagc gagccgttcc accgctcccc
     1681 ttcccggaac cggttgcccg ggttctcggg gatgtggtcc tcgctggagc actggcggga
     1741 gagcatcgcg gcgcagcggc cactggcggc ctccgtgatg tccagcacgt accgtgtgtt
     1801 gtattccccc tcgtcggcgt ccacccacgt cgatacccat cgcccgtcga tggtgggcct
     1861 ttccctgctc ggcacctcga gcggcgtctg gtcgcgcgcg gcgaacacga gcgcgagtgc
     1921 aagtaggtat ctcatggatc cgtggggttg atcaatcatg gaatgccgca ctcgccgggg
     1981 gagtggcgcc agcggtgaat ctcccgcggt tccgcaggcc aatgtcacgg ccggctgtgg
     2041 atcaatcccc gcgggccttc ggacggctcc gccgctggcg aacgatccag agcgcgatgc
     2101 ccatcgccgc ggcaaccagg agcaccgcaa ggttggccac gtgcacgccc aggccggccg
     2161 tccacagagc ggcacgcagg ccggccagca ggaagcccgc tgcgagccag aggtccaggc
     2221 gccgaactcc cggatcagag gcgtccggac tcgcgcccat ccacccgagc aggactccgg
     2281 cggccaggaa cagggaggct tccacggcca cgacccggcc catgtcgaac ccgaagccga
     2341 cacgcgccgc gaggtcgagc agggtgacga ggaacacgac cccgagcggg cgccgatatc
     2401 gtcggagagt tcccatccgg ggggagtgag tatgtcactt agtgaccgcc ggccggaacc
     2461 gcaagagcga tcccgcgcca cggttgcccc ggcggcgccc cgggggtaac atcggccgtc
     2521 cattcagcct gccggttcca tgcgccgcac gatcctgctc cttgccgccc tggcgctcgc
     2581 gccgggcgcg ttcgcccaga ccatcgatcc ggccctcttc gccggactgg agtaccgcga
     2641 ggtggggccc taccgcggcg gacgcagcgc cgccgtcacg ggcgtgcccg gcaggcccat
     2701 gctgtactac atgggcgcca ccgggggtgg tgtctggagg acggaggatg gcggcggatc
     2761 ctgggagagc gtttcggacg ggtatttcgg cggctccata ggggcggtcg ccgtttcgga
     2821 gtgggatccc aacgtgatat acgtcggcgg cggcgagaag accgtgcgcg gcaacgtctc
     2881 gcacggcgat ggggtgtgga agtcggtcga tgccgggaag acgtggacgc acgtcggtct
     2941 cgccgacacg cgccaggtcc cgcgcatccg gatccacccc cggaacccgg atctcgtcta
     3001 cgtggcggcg ctcgggcacc tgtacggacc gaacgacgag cggggcgtct tccggtcccg
     3061 ggacgggggc gccacctggg agaggatcct gttcgtgagc gacggggctg gcgccgtcga
     3121 cctggtcatg gatccgacga acccccgcat cctgtatgcc gcaacgtgga aggtcttccg
     3181 gacgccgtgg ctgctctcgt cgggaggcga aggatcggcg ctctggaaat cgaccgacgg
     3241 cggcgactcg tggacggagc tcacggggaa gggcaacggc cttcccgagg gcacgctggg
     3301 catcatcggc gtcgcggtgt cgccagtgcg ccccgagcgt gtctgggcga tcgtcgaggc
     3361 cgaggacggg ggcgtcttcc gctcggagga cgggggcggc acgtggcgcc gtgtgaacga
     3421 cgaccggaat cttcgccaga gggcctggta ttacacgcgg atctacgcgg gtaccgggga
     3481 cgtggacgac gtctgggtcc tgaacgtcgc gctctggcac tccaccgacg gaggcgcgac
     3541 gtacgagcag gtgcggaccc ctcacggcga ccaccatgac ctctggatcg cacccgagga
     3601 cgccgatcgg ctcgtcgtgg gcgacgacgg cggcgcgcag gtctcgctcg acggcggggc
     3661 gagctggtcc acgatgtaca accagccgac ggcccagttc taccgtgtca cgaccgacaa
     3721 ccacttcccg taccgcatct acggcggaca gcaggacaac tcgacggtgc gcatcctgca
     3781 cacttccgac ggacgcagcg agcgcgagtg ggagccgacg gcgggagggg agtcggcctg
     3841 gatcgcggtg gacccgacga accccgacct ggcgtacggc ggttcctacg gaggctacct
     3901 ggagcgcgtc gaccacgcga ccggccagtc gcgaaacgtc aacgcgtggc ccgacaatcc
     3961 catggggcac ggggcggagg attaccgctt ccgcttccag tggaatttcc cgatccggat
     4021 ctcgcggcac gatccgtcgg tcgtcctgac gggcgcgaac gtgctcttcc gctcggagga
     4081 cgggggccag tcgtggacgg caatctctcc tgacctcacc cgcgcggagc cgtcgaccct
     4141 cggcccgtcc ggaggaccga tcacgaagga caacacgtcg gtcgagtact acggcaccat
     4201 cttcaccttc gcggaggggg aggagccggg ggtcatctgg acgggctcgg acgacggcct
     4261 gatccacctg acccgcgacg gcggggccac gtggacggac gtgactccgc ccgcgcgcgt
     4321 cttccccgag tggatgcagg tgaacgacat ggtgcccgat ccgcacaacc ccgccggcct
     4381 gtacgtggcg ggcacgcggt accggtcgga cgacttctcg ccgtacctct acaggaccgc
     4441 cgactacggg gcgacctgga cgcggatcga ccagggcatc gaccgcatgc acttcacccg
     4501 gacgatcgag cccgatcccg tgcgcgccgg gctgctgtat gcgggcaccg agaacgggat
     4561 ggtcatctcg tttgacgacg gtgcgtcgtg gagccctttc cagctgaacc tgccgcaggt
     4621 gccgatcacg gatctcgagt ggaaggatca cgacctggtg gtcgccacac agggccgcgg
     4681 gttctggatc ctggaggacc tgacgctcct gcaccgcctg cagccggccg acgcggagcg
     4741 cgacttcgtg ttctacggcc cgcatgccgc cacgctgggg gcggagagcg ccgtccgcct
     4801 gcgcttctgg ttcgcggcgg cgcccgactc ggaggccgtc gtacggctcc ggatcctgga
     4861 ggacgacggc gacgtggtcc ggacgttctc gtcgggcggc ccggacgggg agagtctcga
     4921 accgaagctg ggcgtgaaca cgttcgagtg gaacatgcgc tatccggacg ccgagtcctt
     4981 cgacggcctg gtgatgtggt cgggtagtgt gcgcggtccg cgtgcggcgc cgggtgcgta
     5041 ccgggcgcgg ctcgtggtcg gcgacgattc gacggaagtg ccgttcgaaa tccggcccga
     5101 cccgaggtcc gcctcgaccg aggccgacct ccgcgggcaa ttcgagtttc tcctcgggat
     5161 ccgtgacaag ctctcggaga tccaccgggc catcagggaa atccgggcga tccgcgagca
     5221 gaccgcgggc gtcctcgagc gcgtgccggc cgaccatccc gaccgcgaca cgctccgcgc
     5281 ggccgccgac gccatgaacg ccgccctgcg gagcgtcgag gaggcgctct accaggtcaa
     5341 gaaccgttcc gggcaggacc cgctgaactt cccgatccgg ctgaacgaca agctggcggg
     5401 cgtggcctcg accgccgcga cgggacatta ccggcccacc gaccaggcgg tcgcggtgcg
     5461 ggacgagctc gtggcgcgga tcgacgcgga gctcgatcgc atgcgggcgg tgctcgaggc
     5521 cgacctctcc gccttcaacg cgctggtccg cgcggccgac atccccgccg tcgcccgatg
     5581 accacctcgc gcatgatgcg ggcgaccgac cggacactca aggccgccgt ggcgcttttc
     5641 gtgctggcgg gattgacggg cgcggtgttc cgcatggccg gcgcgtacgg gtggacgctg
     5701 ggattcaacc cggtgaacat ccgccacgcg cactcgcacc tgatgtactt cgggtgggcg
     5761 attcccgccc tcttcgcact cgtcgcggcc cgcgtggtcc ggtcgggagg gcgacctccc
     5821 tcgtgcaggc tgctgtcctc ctccttcatc gtggccgttg ccgcgtggga gtccttcctg
     5881 ctctggggct acgaatcggc ccgcatcggt ggggcgcgca ttccggtcag catcatcgtt
     5941 tcgacgctcg ccatgctgct ctggtactgg ttcgcacact ggtaccggcg cggccgggag
     6001 cacctggagg ccgggtccgc gcggacgctt ctcgatgttt cggtcgtgct gctcgtggtg
     6061 tcgacgttcg gcgcgtgggg cgtcgccgcc accgtgccgg ccggcgaatc gctcttcgcc
     6121 ctgaagacgg ccttcaccca cctgttcctc gcgctgttct cggagggctg gctggtcacc
     6181 gcgctgctgg gcctcgcgtg ggcggagctg ccctccggcg cgcggatgcc gcgctgggcg
     6241 ctgccgcttc tcctggccgg cacggcagcg tccttcctcc tgggcatgcc gggccatctc
     6301 gtgggcgaga acctgcgcct cgtggcccgg ctcgggggca tggcctgggg cgtgggggcg
     6361 gtcgggatcg tctggggctg gtggagagcc gccacggccc cgctccgggc ctcctggggc
     6421 tttccgatgc tgttcctggc gctgaaggga tcggtccagg cggcgtcgat gctcgttccc
     6481 ggcctgtggt gggcggacat tcccgccatg cgggtgctgt acctgcacgt gctcctgctc
     6541 ggcttcgtga cgctcgccat cgcggcatcg ctcgtccgcg gccctcggct cgcggccgtg
     6601 caggcggcgg cgctcctcgt catcctgtcc ctcgcgccca tgaccgacct gtggccgggc
     6661 ttcctctcgc tcgaccggcc gacggaggtg gccgcctggg tatcgctcgt cctgcccgtg
     6721 gcggcgatgc ttctctggcg gcgcgcgtac ttcggcggcc gggaggtgcc gggactcgga
     6781 cttcgcgacg gagcgccagg ccggagtctg cccgccccgg gtgacgcggc ctagatcacg
     6841 gcggcgccgc cccgtgcccc gtccagccgc accgagagct cgggcacgcc gaaagacgtt
     6901 ccgacgccca gggcggcgcg gatgagcggg gcgtccaggg gcaccttccg cacgcggtcc
     6961 gcgacctcgt cgaggggcac ggacgtgaac tggccgttct gcacggccac catgcggccg
     7021 aattccccgg ccgcgaccag gtcggtcgcg atggctccga acgaggtcgc caggttgcgg
     7081 tcgtaggccg tgggcgttcc gccgcgctgc acgtggccca ggatcgtcgt cctgacctcg
     7141 ctcttgatct cggcctcgag cgccgcctgg agcacgtacc cgatgccgcc gagccggatc
     7201 gggtcggggc tgtcggcgag cgtgcgctgc acggccatct ctccgcccgt gatgcgtgcg
     7261 ccctcggcga tcacgatgat ggtgaacgac cggccgaaac tctcgcggtc gcggcagacg
     7321 cgggccacct cgtccaccga gaactcgaac tccgggatca ggataaggtc cgccccgccc
     7381 gccacgcctg cgtgcagggc aatccagccc gcgtaccggc ccatggtctc cacgatcatg
     7441 acccgctgat ggctctgcgc cgtggtgtgc aggcggtcga cggcctcggt cacgatcgag
     7501 acggccgtgt cgaacccgaa cgtgcggtcg gtcgccacca ggtcgttgtc gatggtcttc
     7561 ggcaccccga ccatgggcag gcccatctgg ttcaggcggt tcgcgatgga catcgtgccg
     7621 tcgccgccga tggcgacgat gccgtcgagc ccgagctgct tcgcgtacgc gatgacctgc
     7681 gaggagacgt ccgcaccgcc ccgcttgtag tagcggaagg ggttcgcctt gttgttcgtc
     7741 cccaggatcg tgccgcctcg ggtcaggatc ccgctcacgt cgccgtacga gagcggcttg
     7801 atgcgctggg cgatgaggcc caggaagccg tcctcgaagc cgaccacctc ggcgtcatgc
     7861 ttcaggatca ggctcttggt caccgcgcgg atgaccgcat tcagcccggg cgcgtcgcct
     7921 cccccggtca ggaggcccac tcgaagtcgc ttcgccatga atttccgcgg cacatgtcag
     7981 aatgccccga aaacgcgcgg tccgggcgca gggtccgccc ggggactcgc cggcccggtt
     8041 tgactgcgcg gacgtgtgcg gatatcttgg cgccgccggc acagccccgt cccacaacga
     8101 ggggtcgccg gaccggcggg atcactcccg tacccctgca tgagctactg tgtaccggga
     8161 atccggatct ccacctgggc gtggctccgg attgcgtgct tcgtcgccct ggcgcatccg
     8221 gcgaacgggc agtcgctccg cgtttccgcc ccgaaccgcg cttccgcggg ccgcgaggtg
     8281 gcgttctacg tcgggctcga ggagggtcgg cgcttcgacg gtgccgtgct cgcgatgccg
     8341 atggcgtggt ccctggtccg cgcggtggtc gtcggcggcg acggcggcac gatggacctg
     8401 gagccgtgga cggacgagcg ggcgcccggg cggacccggc tcgatccgac gggatccgtt
     8461 cgcgagccga ccgagcttcg gctccacgtg cggctcgggc gcgagcccat ggacggccgg
     8521 tggtccctcg ttcccctcga gcgcgcggcg ggctgggacg acgtcggctt cgtggtgcgc
     8581 gagagcgggc gcgtgacgcg cacgctgcgc gtggacgagc cgcagtcgtc cggtgagaac
     8641 cgggtcctcc ggatcggcga cggcgggccg cccgtacccc tcgacggccg gcggcttccg
     8701 gacctcggga cctcgaaggc gtggacgctc gagacctggt tccggaccgt tgcgctcgac
     8761 gcagtcctcg tatccacgtg ggacggggac gaggcgacgg actacccggc cgagctggtc
     8821 atcgacccgg tcggccggct cgtgttcttc cagggcctcg gcggccgcca ccgctccctc
     8881 aggggaccgc aggccgtcgc ggacggggga tggcaccacg ttgccgtcac gcacgatccg
     8941 gatcgcggat ggacccgtct gctggtggac ggagcggccg tcgactcgct ccatggattc
     9001 gacatgcacg gcatcgcgcg ccccgggcgc gtggtgctcg ggagccggac cgcccggcgt
     9061 ccggacgacg gcatcctgga cttcgcgggg gagatggacg agttccggat ctgggcggcg
     9121 tcccggaccg cccggaacgt gcgcgacggc atgagccggc ccctggccca gccgcccggg
     9181 ggcgcgctca tcctgggctt cgaccggggg atcgagccgg gcctcgttgc gggcgacggc
     9241 gtccgggcac tcatcgtacg atccgacctc acgttccggc gcccggtgcg cgacctcgcg
     9301 gtccggatga cggccggata cgtggagctg tcctggtcgg gcgacgaggg aacgggaacc
     9361 atctaccacg tggagcgatc ggcggacggt gccctcttcg agcccgtcgc ccgctacacg
     9421 aacgcgcagg ggtcccccgg ggcggttagc ggctcgcggt cattccgtca cggggagcac
     9481 gcgacggagg gtcgcgtggc ctggtacagg atccggcagg tcgaggccac gggagcggag
     9541 aacctgaccc cggtcgtgaa ggtgggcgtc ggctcggcgc acgcggtgag ccgggccacg
     9601 ctcgagggtg cctggcccaa tccgttcaat ccgaacacga cgatcgggta ccgcgtggac
     9661 cggggcggcg aggtcgcggt ttcggtgtgg gatctctccg gccagcgggt ccgcgtgctc
     9721 gcggacgcgt atcatgagcc gggacagtac acggtgtcct tcgcggccga agagctgccc
     9781 agcggcgcct atttcgttcg actcgagacg gtcgacggca tccagatccg ccagatcgtc
     9841 ctcgccaaat agcggccggc aggtcgcggc ggagagcgcg gcgcctctcc gcccgaagcc
     9901 gcgacccgca cggccgggac tgaccgccat gaatctcacg tatttcggac actccgcctt
     9961 ccggatccgc gtggacgggg cgacccttct cgtcgatccc ttcatctcgg gcaatccgct
    10021 cgccgagccg gtcgtgacgg ccgccgaact gtcggccgac gtcattctgc tcacccacgc
    10081 gcacggcgac cactggggcg atacggcgtc catagcgaag cggaccggcg cgctcgtcgt
    10141 cgccaacttc gagatcaccg aggttctccg gaagaagcac ggtcacgcga acgtgcacgc
    10201 gatgaatacc ggaggatcgg tcgacttccc g
//
LOCUS       DBNHQZ010000248         5105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1700870, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000248 DBNHQZ010000000
VERSION     DBNHQZ010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1700870"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2245
                     /locus_tag="AC16YU_06670"
     CDS             <1..2245
                     /locus_tag="AC16YU_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR6064943.1"
                     /translation="DSRRDNLRRTGEEEGDPTGALPAIVWLSYNVHGNEAVSTEAAMQ
                     TLYDLARTDHPDSREWLESVVVVIDPCLNPDGRERYVSWYNRTVGAIPDPDPAAVEHH
                     EEWPGGRTNHYFFDLNRDWTWLTQQETRARIAQYNRWMPHVHVDFHEQGVDEPYYFAP
                     AAEPYHTVVSAWQREFQREIGTNHAEWFDSSYWLYFTRERFDLFYPGYGDTYPTYVGA
                     IGMTYEQGGSGRAGLGIETAEGDTLTLVERIEHHHATSLSTVQATARHAERVVREFEA
                     WYDAGAGDTRWGTFVLRAASGEDRLAAVRSHLDALGIRSGSTSGTRRVRGHDYRTGRE
                     TTVEIGPGDLIVPVDQPRGVLAGVLMEPDPVLPDSATYDITAWALPYAYGIDAVGLRE
                     AVAIDRPWSAASAALPDGAERPYAYLSGWSSQADLRFLARVLRAGATVRVATEAFRAG
                     GTAHAPGTLVITRAGNARLGERFDGIVLDAARAEGRRLRAVTTGMVESGSDFGSSSVR
                     FVRPPRIAMAFGEGTSSASVGEIWHWFDEQVGYPVTRIPANSLGTVALDDYDVVILPS
                     GSYREPLDDRGIARLREWVAGGGRLVAVGQTLSLLAGRDGFALRRMERPEDPGDSLRS
                     YADRERSALVDAVPGSVFSTRVDATHPLGFGLERGYFTLRLGSAAYAYLAGDRAWNVG
                     VVQKDARRAGFVGHRAFSRLENTLAFGVEEIGRGAVVYLVDDPLYRAFWYEGRLLFGN
                     AVFMPLR"
     gene            complement(2229..4037)
                     /locus_tag="AC16YU_06675"
     CDS             complement(2229..4037)
                     /locus_tag="AC16YU_06675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064944.1"
                     /translation="MRSEPEAERPAPDRMWSALRRVFALARPYRGRLGLAVALTAVGS
                     LVWLIVPLGLRTLIDAVFEEGNRSLLDLMTAGLLVLFLVQAGVSFGGTYLLGTVGARI
                     VTDLRRRVFDHLSRLDFGYFARQRTGELTSRLTNDVGAVREAVTHALTELLSQSISLM
                     GSLGLMVVLNWRLSAVVFLVVPGVTLLARKLGRAMRRYARTVQDQLADTTAIAEEAIA
                     AIRVVKAFTREDHESGRYGDAAEALFGTERKRILVRASYSAAIGALFFSALVGIFWFG
                     GTEVLADRLSAGDLVAFIFYAMNISRSVGGMSSLYATFSSVTGATERLFEVLDTHPEV
                     QDRPGARPVGAVHGNVAFHGVGFAYVADRPVLHDISFSVRAGQTVALVGPSGAGKTTL
                     VHLIPRFYDPTEGSVTLDGVDLRDLRVESLRQQIAVVPQDLYLFNASVRENVRYGRLD
                     ADDRAIEEAVRDANAGDFVAALPDGLDTVIGERGITLSGGERQRVAIARALLRDAPLL
                     LLDEPTSSLDSASEALIQEALDRLTAGRTTFIIAHRLSTVRHADLILVVENGRIVQSG
                     THDSLIAEGGLYGALAARQFRDEPAGPASAPVSEGA"
     gene            complement(4058..4495)
                     /locus_tag="AC16YU_06680"
     CDS             complement(4058..4495)
                     /locus_tag="AC16YU_06680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6064945.1"
                     /translation="MTGEPTTKVRAAGGILARSHDGRPEILLIRRRDAWDLPKGKAKE
                     GESSRRCALREVREELGIDRVTIVEPLGTTRHAYRENGRHIRKTTRWYLMRTDATRFF
                     PQAEERIDSVAWVHPDEAARRLAYESLRELVARHRERLVEGPA"
     gene            complement(4485..>5105)
                     /locus_tag="AC16YU_06685"
     CDS             complement(4485..>5105)
                     /locus_tag="AC16YU_06685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6064946.1"
                     /translation="DFGLLHAWTGDGAGRVVAALLILDASNYAWHRLCHAVPFLWRFH
                     RVHHSDPRMDVSTSGRYHVGEVVLSSVLWIAVIALFGIRLWEMLLFELLSAAVAQFHH
                     ANISLPPAVDRLLRVFVVTPGMHRVHHSRLKHEMDSNFSSFLSVWDRLGRSYRATDRP
                     QDIAFGLDRLEAPDRQTLVGLLGMPLARTPGPDGPSTNPSDAAHDR"
BASE COUNT          764 a         1756 c         1879 g          706 t
ORIGIN      
        1 cgacagccgg cgcgacaacc tgcgccggac gggcgaggag gagggcgacc ccacgggcgc
       61 gctcccggcc atcgtctggc tgagctacaa cgtgcacggg aacgaggcgg tcagcacaga
      121 ggccgccatg cagacgctct acgacctggc ccgcacggat cacccggact cccgggagtg
      181 gctggaatcc gtcgtggtcg tcatcgaccc ctgcctgaat ccggacggcc gcgagcgcta
      241 cgtgtcgtgg tacaaccgca cggtcggagc gattccggat ccggatccgg ccgccgtaga
      301 gcaccacgaa gagtggcccg gcggacggac gaaccactac ttcttcgacc tgaaccggga
      361 ctggacctgg ctcacgcagc aggagacgcg ggccaggatc gcgcagtaca accgctggat
      421 gccccacgtc cacgtggact tccacgaaca gggcgtggac gagccgtact acttcgcgcc
      481 cgccgccgag ccgtaccaca cggtcgtctc ggcctggcag cgcgagttcc agcgggagat
      541 cggcaccaac cacgcggagt ggttcgactc ctcgtactgg ctctatttca cccgggagcg
      601 cttcgacctg ttctacccgg gctacgggga cacgtacccg acgtacgttg gtgcgatcgg
      661 catgacctac gagcagggag ggtccggccg cgcggggctc ggtatcgaga ccgcggaggg
      721 ggatacgctc acgctcgtcg agcggatcga gcaccaccac gcgacgagcc tctcgacggt
      781 ccaggccacg gcgcggcacg cggagcgcgt cgtgcgtgag ttcgaggcct ggtacgacgc
      841 gggcgccggg gacacgaggt gggggacgtt cgtcctccgc gcggcgtcgg gcgaggaccg
      901 gctggccgcg gtccgctcgc acctggacgc cctgggcatc cggagcggat ccacgtccgg
      961 gacgcgccgc gtccgggggc acgactaccg caccgggcgc gagaccacgg tcgagatcgg
     1021 gccgggcgac ctcatcgttc ctgtggacca gccgcgcggc gtgctcgcgg gcgtcctgat
     1081 ggagccggac ccggtcctcc ctgactccgc cacgtacgac atcacggcct gggccctccc
     1141 ctacgcgtac gggatcgatg ccgtgggcct gcgggaggcg gttgcgatcg accggccgtg
     1201 gtcggccgcc tcggcggccc ttccggacgg cgcggagcgc ccctatgcgt acctgtcggg
     1261 gtggtcctcg caggccgacc tgcgcttcct ggcccgggtg ctccgcgccg gcgcaacggt
     1321 acgcgtggcc acggaggcgt tccgggccgg cggcacggcg catgcgcccg gcacgctcgt
     1381 catcacccgc gcgggtaacg cccgcctggg cgagcgcttc gacgggatcg tgctcgatgc
     1441 tgcgcgcgcc gagggacgac ggctccgcgc ggtcacgacc ggcatggtgg aatcgggatc
     1501 cgacttcgga tcgagctcgg tgcggttcgt ccggccgccc cgcatcgcga tggcattcgg
     1561 ggaagggacg tcgtccgcct ccgtgggcga gatctggcac tggtttgacg agcaggtcgg
     1621 ctacccggtg acccggatcc cggcgaactc gctcggcacg gtcgcgctcg acgattacga
     1681 tgtcgtcatc ctgccgtcgg gctcgtaccg ggagccgctc gacgaccggg ggatcgcgcg
     1741 acttcgagag tgggtcgcgg gaggtggaag gctcgtggcc gtggggcaga cgctgtcgct
     1801 cctcgccggc cgggacgggt tcgcgctgcg gcgcatggag cggccggagg acccgggcga
     1861 ctcgctccgt tcatacgccg accgggagcg ctcggccctg gtggatgcgg tacctggatc
     1921 cgtgttctcg acacgggtcg acgcgacgca ccctctcggc ttcggtctcg agcgaggcta
     1981 tttcacgctc cggctcggct ccgccgccta cgcctacctg gcgggtgacc gggcgtggaa
     2041 cgtgggggtc gtccagaagg acgcgcgccg cgccgggttc gtgggccacc gggcgttctc
     2101 gcgcctggag aacacgctcg ccttcggcgt cgaggagatc ggccgcggag ccgtggtcta
     2161 cctggtcgac gatccgctgt accgggcgtt ctggtacgag ggccgcctgc tgttcgggaa
     2221 cgccgtgttc atgccccttc gctgacgggg gccgaagccg gccccgcggg ctcgtcgcgg
     2281 aactggcggg cggcgagggc gccatagagg ccgccctcgg cgatgagcga gtcgtgcgtt
     2341 ccggactgga cgatccggcc gttctccacg accaggatca ggtcggcgtg ccgcacggtc
     2401 gacagccggt gcgcgatgat gaaggtcgtg cgccccgccg tcagccggtc cagggcctcc
     2461 tggatcaggg cctccgaggc ggagtcgagc gaggacgtcg gctcgtcgag cagaagcagc
     2521 ggcgcatcgc ggagcagggc gcgcgcgatg gccacccgct ggcgctcgcc gcccgagagc
     2581 gtgatgcccc gctcaccgat cacggtatcc aggccgtcgg gcagggccgc cacgaaatcc
     2641 ccggcgttgg cgtcgcgcac ggcctcctcg atggctcggt cgtctgcgtc gagccggccg
     2701 tagcggacgt tctcgcgcac cgaggcattg aagaggtaca ggtcctgcgg gacgacggcg
     2761 atctgctgcc gcagggactc gacccgcagg tcgcgcaggt ccacgccgtc gagcgtcacg
     2821 gagccttcgg tgggatcgta gaaccggggg atgaggtgga cgagcgtggt cttgcccgcg
     2881 ccgctcggcc cgaccagggc gaccgtctgt ccggcccgga ccgagaagga gatgtcgtgc
     2941 aggaccggcc ggtccgccac gtaggcgaat ccgaccccgt ggaacgcgac gtttccgtgc
     3001 accgcaccga cggggcgggc gcctgggcgg tcctgcacct ccgggtgcgt gtccagcacc
     3061 tcgaacaggc gctcggtggc tcccgtgacg ctcgagaagg tcgcgtacag gctggacatc
     3121 ccgccgaccg accgcgagat gttcatggca tagaaaatga acgcgaccag gtcgcctgcg
     3181 gacagccggt cggccagtac ctcggtgccc ccgaaccaga agatgcccac gagggccgag
     3241 aagaagagcg cgccgatcgc ggcgctgtac gaggcccgca cgaggatccg tttgcgctcg
     3301 gtaccgaaga gcgcctcggc cgcgtcgccg tagcgccccg attcgtgatc ctcccgggtg
     3361 aatgccttga ctacgcggat ggccgcgatg gcttcctcgg cgatggcggt cgtgtcggcg
     3421 agctggtcct gcacggtccg ggcatagcgg cgcatcgcgc ggcccagctt ccgggcgagg
     3481 agcgtcactc caggcaccac caggaacacg acggcgctca gccgccagtt caggacgacc
     3541 atgagcccga gcgagcccat gagcgagatc gactgcgaga gcagctcggt cagcgcatgc
     3601 gtgacggcct ctcggacggc gccgacgtcg ttggtgagcc gggacgtcag ctcgccggtg
     3661 cgctggcgcg cgaagtagcc gaaatcgagg cggctcaggt ggtcgaagac gcgcctccgc
     3721 aggtcggtca cgatgcgcgc gcccaccgtg ccgagcaggt acgtcccgcc gaacgagacc
     3781 cccgcctgca cgaggaacag gaccagcagg ccggcggtca tgagatcgag cagggaccgg
     3841 ttcccctcct cgaacactgc gtcgatgagc gtgcgcagcc cgaggggcac gatgagccag
     3901 acgagcgagc ccacggcagt gagcgcgacg gcaaggccga gccgaccgcg gtacgggcgg
     3961 gcgagcgcga atacccggcg cagcgccgac cacatccggt ccggcgccgg ccgctcggcc
     4021 tccggctccg agcgcatggc cctgcggacg ggcctcatca cgccgggccc tccacgaggc
     4081 gttccctgtg ccgggcgacg agctcccgca gggactcgta ggccagtcgg cgggccgcct
     4141 cgtcggggtg cacccacgcg accgagtcga tccgctcctc cgcctgcggg aagaaccgcg
     4201 tggcgtcggt tcgcatgagg taccagcggg tggtcttcct tatgtgccgc ccgttctcgc
     4261 ggtaggcgtg ccgcgtggtg ccgagcggct cgacgatggt cacgcggtcg atgccgagct
     4321 cctcgcgcac ctcgcgcagc gcgcaccggc gcgacgactc cccctcctta gccttgccct
     4381 tcggaaggtc ccacgcgtcc cgccgtcgga tcagcaggat ctccggccgc ccgtcgtggc
     4441 tccgggcgag gatgccgccg gccgcgcgga ccttcgtggt cggttcaccg gtcatgggct
     4501 gcgtcggaag ggttagtgga aggaccgtcg ggccccgggg tccgcgccag cggcatgccc
     4561 aggaggccga ccagggtctg ccgatccggg gcttccaggc ggtccagtcc gaaggcgatg
     4621 tcctgcggcc ggtccgtggc gcggtacgag cggcccaggc ggtcccatac ggaaagaaac
     4681 gagctgaagt tcgaatccat ctcgtgcttc aggcgcgagt ggtgcacccg gtgcatcccg
     4741 ggcgtcacga cgaagacgcg cagcaggcgg tcgacggcgg gcggaaggga gatgttcgcg
     4801 tggtggaact gcgcgaccgc ggccgagagg agctcgaaga gcagcatctc ccagaggcgg
     4861 atcccgaaga gcgcgatcac ggcgatccag aggacggacg acagcacgac ctcccccacg
     4921 tggtaccggc cggacgtgga gacgtccatg cgcggatcgg agtggtgcac gcggtggaag
     4981 cgccacagga agggcaccgc gtggcacagg cggtgccagg cgtagttcga ggcgtccagg
     5041 atcagcagtg cggcgacgac gcgcccggcc ccgtcgccgg tccaggcgtg cagcaggccg
     5101 aagtc
//
LOCUS       DBNHQZ010000249         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_752718, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000249 DBNHQZ010000000
VERSION     DBNHQZ010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_752718"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC16YU_06690"
     CDS             <1..251
                     /locus_tag="AC16YU_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR6064947.1"
                     /translation="RRFADRAEALGAVAVVSATFETGIGLYGLAALAASLPGGAVPAG
                     LGTAAFLSGDTVTPRFPADAPVVRLPAPYTVHPSPDAA"
     gene            248..1648
                     /locus_tag="AC16YU_06695"
     CDS             248..1648
                     /locus_tag="AC16YU_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6064948.1"
                     /translation="MTHVLPPDPLRHAAGLWPDLPALEHGAGTATFAGLDVAVDRVCR
                     VLARHGARPRRPVLSVEADVALHVTILLALLRLGAIAVPVNHRLPAEGIDELAARLAS
                     PLRVGPAGSAVDADELRAAAAGAEPAPPVHPPAGARRRATIFFTSGSAGRPKAVVHRL
                     AAHLRSADAAALHLGLGPGDRWLATLPLNHVGGLAPVFRALRSGATLVVPDGPPTATL
                     LRSTRTTHVSLVATQLRRLTAPGSSAPPALRCVLLGGGPADPSLLRDAFEDGWPVVGS
                     YGMTESASLVAATRPGERDPMAAGRPLAHARIRIGPGGEILLRGASLAEGYLEGGRVA
                     SLTDRSGWFHTGDRGSLGVGGRLTVEGRLDRRFVSGGENVQPEEIERRIAEIPGVRAV
                     IVVPVPDPEYGQRPVAFIDGPVDPEAIRVALRRRLPSFLVPDAFFPMPDAPGGAGIKV
                     SPTRLREIALRKAGRG"
     gene            1750..2196
                     /gene="mscL"
                     /locus_tag="AC16YU_06700"
     CDS             1750..2196
                     /gene="mscL"
                     /locus_tag="AC16YU_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR6064949.1"
                     /translation="MLKEFKEFAVKGNMVDMAVGIILGAAFGTVVKSVVDDILMPIVS
                     AVSGAPDFSNLFVILKDPVDMAGVNMESIAAVREAGGVALGYGLFINAAIAFLIVAFV
                     LFMVIKAMNKLKRKEEAAPAPPPAPPEPSAEEKLLTEIRDLLRARA"
     gene            2279..3076
                     /locus_tag="AC16YU_06705"
     CDS             2279..3076
                     /locus_tag="AC16YU_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6064950.1"
                     /translation="MDLYYREYGSGPPLVILHGLLGAGGNWHTLARRVFGATHRVLVP
                     DQRNHGRSPHGDAFDYATLAEDVEDFMNARSLSTTALLGHSMGGKVAMEFALTRPERV
                     ERLVVVDIAPRAYPDRHRPILDAMRAVDPTTMERLSEVRDAVRPLIRDPDVVELIVKN
                     VERGDDGRMAWAVNLDAIEGHYRHIMDGLDTWATYDGPALFLRGERSDYVGEEDMETI
                     RAYFPNAILDTVPDAGHWVHADHPAAFARAVRRFLGVPDHAPGGKGP"
     gene            3131..>3403
                     /locus_tag="AC16YU_06710"
     CDS             3131..>3403
                     /locus_tag="AC16YU_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6064951.1"
                     /translation="MGSADRIRLLHEAIERRILVIDGAMGTMIQREKLGEEAYRGDRF
                     RRHKGELRGNNDLLTLTRPDLVRDIHDAYLQAGADIIETNTFTSTSV"
BASE COUNT          442 a         1254 c         1202 g          505 t
ORIGIN      
        1 cccggcgctt cgccgatcgg gccgaggcat tgggcgccgt cgccgtcgtc agcgccacgt
       61 tcgagacggg catcgggctc tacgggctgg cggccctggc cgcctccctt cccggagggg
      121 ccgttcccgc cgggctcgga acggcggcgt tcctgtccgg agacacggtg acaccccgct
      181 tcccggccga tgcgcccgtc gtgcggctcc cggcgccgta cacggtgcac ccttccccgg
      241 acgccgcatg acgcacgtcc ttcccccgga ccccctccgc cacgcagccg gcctatggcc
      301 ggacctgccg gccctcgagc atggcgcggg gacggccacg ttcgccggcc tggacgttgc
      361 ggtggaccgg gtgtgccggg tgctcgcccg ccacggcgcc cggccccggc gaccggtcct
      421 gtccgtcgag gcggacgtgg ccctgcacgt gacgatcctc ctcgccctcc ttcgcctggg
      481 cgcgatcgcc gtccccgtga accacagact gcccgcggaa gggatcgacg agctcgccgc
      541 caggctcgct tcgccgctcc gcgtggggcc ggcaggctcg gccgtggacg cggacgagct
      601 gcgggccgcg gcggccggtg ccgagcccgc gcccccggtc cacccgccgg ccggtgcacg
      661 gcgccgcgcg accatctttt tcacgtccgg ttccgccggc cggccgaagg cggtcgttca
      721 ccggctggcg gcccacctcc gaagcgcgga tgcggcggcg ctgcacctcg gactcggccc
      781 tggcgaccgc tggctcgcga ccctgcccct gaaccacgtc ggcggcctcg ccccggtatt
      841 ccgggcgctc cggtcgggcg cgacgctcgt ggttccggac ggtcccccga ccgccacgct
      901 cctgcggtcc acccggacca cgcacgtgtc gctcgtggcg acccagctcc ggcgcctcac
      961 cgcgcccgga tcgtccgctc cgccggccct gcgctgcgtg ctgctcggtg ggggaccggc
     1021 ggatccgtcg cttctccgcg atgccttcga ggacggctgg ccggtggtgg gcagctacgg
     1081 gatgaccgag tccgcgtcgc tggtcgcggc cacacgcccc ggcgagcgcg atccaatggc
     1141 ggccggtcgg ccgctcgccc atgcccgaat ccggatcgga cccggcgggg agatcctgct
     1201 ccggggggcc tcgctcgccg aggggtacct cgagggcgga cgggttgcga gtctcacgga
     1261 ccggtccggg tggttccaca ccggcgaccg gggctcgctc ggcgtgggcg ggcgcctgac
     1321 cgtcgaaggc cggctggatc gccggttcgt ctcgggcggc gagaacgtgc agcccgagga
     1381 gatcgagcgc cggatcgcgg agattccggg cgtgcgggcg gtgatcgtgg tgcccgtgcc
     1441 cgatcccgag tacgggcagc gacccgtggc cttcatcgac ggcccggtcg acccggaggc
     1501 gatccgggtc gccttgcgcc ggcgcctgcc gtcctttctc gtgccggacg cgttctttcc
     1561 catgccggac gcccccgggg gggccggcat caaggtctcg ccgacccgcc tgcgcgagat
     1621 cgcgctccgc aaggcgggca ggggctgacc ggccccgtcc cgcacttgct aatctgcgcg
     1681 gcgtggccct aattccggtg cggatcgcgc cggcgaccgc ctcgctcaaa cttcatcgga
     1741 aggtggatca tgctcaagga attcaaggag tttgccgtca agggcaacat ggtggacatg
     1801 gcggtcggca tcatcctggg tgccgcgttc gggacggtcg tgaagtccgt cgtggacgat
     1861 atcctcatgc cgatcgtgtc cgcggtctcg ggcgcgccgg acttcagcaa cctgttcgtc
     1921 attctgaagg acccggtgga catggccgga gtgaacatgg agtcgatcgc cgccgtgcgc
     1981 gaggccgggg gcgtcgccct ggggtacggt ctgttcatca acgccgccat cgccttcctc
     2041 atcgtcgcgt tcgtgctctt catggtcatc aaggccatga acaagctgaa gcggaaggaa
     2101 gaggcagctc cggcgcctcc gcccgctccg cccgagccgt cggccgagga gaagctcctc
     2161 accgagattc gcgacctgct gcgcgcgagg gcctgagccg tcggtcgggg ccctctccgg
     2221 gcattctcgc atttcgggcg ggccggtccc cgggcggggc cggcccgccg cgccgttcat
     2281 ggacctttac taccgcgaat acgggagcgg cccgccgctc gtgatcctgc acggcctcct
     2341 cggggcgggc gggaactggc acacgctcgc gcggcgcgta ttcggcgcca cccaccgggt
     2401 gctggtgccc gaccagcgca accacggccg ctcgccgcac ggggacgcct ttgactacgc
     2461 gaccctcgcg gaggacgtcg aggacttcat gaacgcgcgg agcctgtcca cgacggccct
     2521 gctcgggcat tccatgggcg gcaaggtggc catggagttc gcgctcaccc gcccggaacg
     2581 cgtggagcgt ctcgtggtcg tcgacatcgc accgcgggcg tatcccgacc gccaccggcc
     2641 gatcctcgac gccatgcgcg cggtggaccc gaccacgatg gagcggctct ccgaagtccg
     2701 ggacgccgtc cgcccgctga tccgggatcc ggacgtcgtg gaactcatcg tgaagaacgt
     2761 cgagcggggg gacgacggcc gcatggcctg ggccgtgaac ctggatgcga tcgagggcca
     2821 ctaccggcac atcatggacg gcctggacac gtgggccacc tacgacgggc ccgcgctgtt
     2881 cctccgcggg gaaaggtcgg actacgtcgg ggaagaagac atggagacga tccgcgccta
     2941 cttccccaac gcgatcctgg acacggtgcc ggacgccggc cactgggtgc acgcggacca
     3001 tccggccgcg ttcgcgcgcg cggtgcggcg gttcctgggc gttcccgatc acgcgccggg
     3061 cggcaagggg ccgtgaaccg gccggagcca gggtcgttct cccgattcgt gccttccgta
     3121 ccctatgccg atgggctccg ccgaccgaat ccgactcctg cacgaggcga tcgagcgccg
     3181 catcctggtc atcgacggcg caatgggcac gatgatccag cgcgagaaac tgggcgagga
     3241 ggcctatcgc ggcgaccggt tccgcaggca caagggagag ctccggggca acaacgacct
     3301 gctcacgctg acccgtccgg acctggtccg tgacatccac gacgcgtacc tgcaggcggg
     3361 cgcggacatc atcgagacga acacgttcac gtcgacctcg gtc
//
LOCUS       DBNHQZ010000250         3926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2918364, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000250 DBNHQZ010000000
VERSION     DBNHQZ010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2918364"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..733)
                     /locus_tag="AC16YU_06715"
     CDS             complement(176..733)
                     /locus_tag="AC16YU_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064952.1"
                     /translation="MTRLLLPAAMAALLAACSTGAPPPPDRDGTDGTDITEPAATESV
                     DLVDPMIAPAVAGEDGWNYRLTAEADLSGDGTPEQVLLTARVEMVRGRPAWDDGQPWQ
                     VYVEEQDGTRTRLYAQRLQLGTLSMRVAVGDSLAPATVVLLEELPDRLAVYEATYRGP
                     GDATVVAAFERQVDPVGDAASPRLP"
     gene            948..1583
                     /locus_tag="AC16YU_06720"
     CDS             948..1583
                     /locus_tag="AC16YU_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064953.1"
                     /translation="MLPPVLGAARHAGCEDSLRAANARVGRLATVLVAAGLAISAAGC
                     DATFTPLYDDADTAIPDAAAVDQATADKARNDLALIRESVARYGRVEAAEDAGWLLPP
                     GDGCVEHPTLGGMGFHYINFGRVDLELEASEPEVLVFVPGPGGRLTLGAVEFIVPASV
                     WDAEHPGELPGVMGVDLHRHPVLDLYVLHAWVFRHNPSGILTDFNPSVSCP"
     gene            1744..2661
                     /locus_tag="AC16YU_06725"
     CDS             1744..2661
                     /locus_tag="AC16YU_06725"
                     /inference="COORDINATES: protein motif:HMM:NF022588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HIR6064954.1"
                     /translation="MIRPRAFAPLAALFVIAMFAASPVTAQVVASERSTLSQTVDGTV
                     ITIDYSRPSQRGRAPLFGGVMPYGDIITPGANMATTLEVSKDVVIGETPVPAGKYSVW
                     VAFDSADEWQLGLDETWQRFHGPHPTMDELEIAIPVTPREVPMDLETLTFHFPTVFED
                     GSGTTLRLHWGTTAVDVDIHVQPTPMENITAEAAAAYVGRYEVDVVKIPPFTIYEGKV
                     EVELAYEDGFLHSWMEIGPYSAPHDLAFFPQAEQVLYPVELIRGIPANNFGGMVFFEF
                     TKDESGKADTFEARLPQADQLWMSGRRIE"
     gene            2737..3861
                     /locus_tag="AC16YU_06730"
     CDS             2737..3861
                     /locus_tag="AC16YU_06730"
                     /inference="COORDINATES: protein motif:HMM:NF017454.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HIR6064955.1"
                     /translation="MNRTRYVLLLALLLSAGAEARAQEASSVLASDMFIKLDDIPGEA
                     TFQGYERWTEILSYSHAMTAAYDFGGGGGGAGVTRHEPVVFIKRLDKTTPVLFDRMNK
                     GQVLAEVQIDFLRGAEQPFAYLSVRLEDVHITSYSVSASSGDLPWEAISLVYRRIELS
                     YTETNADGSPGETQTVGWDVSTNMPLSIARESIDVRVDGSEVVFLWQTTGEDGVYGYE
                     VQHLEQDGFRRAAYVPASGWSSQALEYEVRIRGLDDGVHVFRLAGLTVDGRISYSDEI
                     LVTLGKPSGYALALDAPYPNPATDRATVAVSMDRYENARISVFDLLGREVAVLHDGML
                     QPGIESRFEFEPSDSLPSGVYVVRASAGELMRSQLLTVMR"
BASE COUNT          608 a         1368 c         1337 g          613 t
ORIGIN      
        1 tacacgggaa cgttcgcgga cggcacctac tcgcttcgcc tgggcgagct cgcgagcgcg
       61 ccggcatgga cgcccgacgt gtggtcctcg gcgcagtaca gggcgggcga cgacccgagg
      121 gacctggtgc tggcgcggga gtagcgggcg caggggcctg cacgacccgg gccggtcacg
      181 gaagccgcgg gctggcggca tcaccgacgg gatccacctg cctctcgaac gcggcgacga
      241 ccgtggcgtc gcccggcccc cggtaggtgg cctcgtagac ggccagccgg tcgggcagct
      301 cttcgagcag gacgaccgtc gccggagcga gcgagtcgcc gaccgcgacg cgcatcgaaa
      361 gcgttccgag ttggagccgc tgcgcgtaca gccgggtgcg ggtgccgtcc tgctcctcga
      421 catacacctg ccaaggctgg ccgtcgtccc aggccggcct gccgcggacc atttccaccc
      481 gcgccgtgag caggacctgt tcgggcgtgc cgtcgcccga gaggtcggcc tccgcggtca
      541 gccggtagtt ccagccgtcc tccccggcca cggccggggc gatcatcggg tccacgagat
      601 cgacggattc cgtggcggcg ggttccgtga tgtcggtgcc gtccgtgccg tcgcggtcgg
      661 gaggcggtgg cgcccccgtg gagcaggccg cgagcagggc agccatggcg gccgggagga
      721 gcagtcgagt catggccgtt cgaacctccg gtccttgcgc tgatcgaggg ccggccggcg
      781 cccgcccgtc gtccggccgt gatctgcgcg gtgtggggta caaaataggg cgcatcgaaa
      841 cgcccgtatg gttgattttc agattgttac ggatacctta cgggtcggga gggaaaccca
      901 gggccctccc gatacggaac ccttcatcac agaacggagg aacgggaatg ctaccccctg
      961 tcctcggcgc ggcccggcat gcgggctgtg aggattcgct tcgtgcggcg aacgcccgcg
     1021 tcggccggct cgccacggtc ctcgtcgccg ccggcctggc gatatcggcc gccggctgcg
     1081 atgccacctt caccccactg tacgacgacg cggatacggc catccccgat gccgccgccg
     1141 tcgaccaggc gacggccgac aaggcccgca acgacctcgc cctgatccgc gaatcggtcg
     1201 cccggtacgg ccgcgtggag gcggccgagg acgccggctg gctgctcccg ccgggcgacg
     1261 gctgcgtcga gcacccgacg ctcggaggca tgggtttcca ttacatcaat ttcgggcggg
     1321 tcgacctgga gctcgaagcc agcgagcccg aggtgctggt cttcgtgccg ggcccgggcg
     1381 ggcgcctgac gctcggcgcc gtggagttca tcgtgcccgc gtcggtatgg gatgccgagc
     1441 atcccgggga gctgccgggc gtcatgggcg tggatctcca ccggcatccg gtgctggacc
     1501 tgtacgtgct gcacgcctgg gtcttccgcc acaatccgag cggcatcctc acggacttca
     1561 acccgtccgt ttcgtgcccg taaacgggcc gcctgagcgg acggggcccg gccggacgca
     1621 ggatcccgcg tcaggctgca acgcccggga attccttccg taggctcgcc ccgcgctcct
     1681 gtcgtccccc ggcgggagcg cgggcaacac ccattctcaa gaacacacac cggaaccatg
     1741 aagatgattc gaccccgggc cttcgcgccc ctcgccgcgt tgttcgtgat cgcgatgttc
     1801 gcggcgagtc ccgtcaccgc gcaggtcgtc gccagcgagc gatcgaccct ctcccagacc
     1861 gtcgacggca cggtcatcac catcgactac tcccgcccgt cgcagcgcgg ccgcgccccc
     1921 ctcttcggcg gcgtcatgcc gtacggggac atcatcacgc cgggagccaa catggccacg
     1981 accctcgagg tcagcaagga cgtcgtgatc ggcgagaccc ccgttccggc tggcaagtac
     2041 tcggtctggg tcgcctttga cagcgccgac gagtggcagc tcggcctcga cgagacctgg
     2101 cagcggttcc acggtccgca cccgaccatg gacgagctgg agatcgccat ccccgtcaca
     2161 cctcgtgagg tcccgatgga cctggagacg ctcacgttcc actttccgac cgtcttcgag
     2221 gacggctcgg gcacgacgct gcgccttcac tggggcacca cggccgtcga cgtggacatc
     2281 cacgtccagc cgacccccat ggagaacatc acggccgagg cggccgcggc ctacgtgggc
     2341 cgctacgagg tcgacgtcgt gaagataccc ccattcacca tctacgaggg caaggtcgag
     2401 gtcgagctcg cctacgagga cggcttcctc cattcctgga tggaaatcgg gccctacagc
     2461 gctccgcacg acctggcgtt cttcccgcag gccgagcagg tgctctatcc cgtggagctc
     2521 atcaggggca tcccggcgaa caacttcggc ggaatggtct tcttcgagtt cacgaaggac
     2581 gagtcgggga aggcagacac gttcgaggcc cgtctgccgc aggctgacca gctctggatg
     2641 tcgggccgcc ggatcgagta gtcggggcgg gtgaaccgga tcgcgacgcg ggcccgatcg
     2701 cagggagggc ttcaccacaa cagaccggga cgaaccatga acagaacacg ctacgtcctc
     2761 ctcctcgcgc tccttctttc cgccggggcc gaggcccggg cgcaggaggc gtcgagcgtc
     2821 ctcgcctcgg acatgttcat caagctggac gacatacccg gcgaggcgac ctttcaggga
     2881 tacgaacgct ggaccgagat cctctcgtac tcccacgcca tgacggccgc ttacgatttc
     2941 ggcggcggag gcggaggcgc cggcgtgacc aggcacgagc ccgtcgtatt catcaagagg
     3001 ctggacaaga cgacgcccgt gctcttcgac cggatgaaca agggccaggt attggctgaa
     3061 gtccagatcg actttctgag aggggcggag cagcccttcg cctacctctc ggtcaggctc
     3121 gaggatgtgc acatcacgtc ctacagcgtg agcgccagct cgggcgactt gccctgggag
     3181 gcgatatcgc tggtgtaccg caggatcgaa ttgtcgtaca cggagaccaa tgcggacggc
     3241 agtcccggcg agacacagac ggtcggctgg gacgtcagca cgaacatgcc cctgtccatc
     3301 gcgcgggagt ccatagacgt ccgggtggac ggctccgagg tcgtcttcct gtggcagacc
     3361 acgggcgagg acggcgtgta cggctacgag gtccagcacc tggagcagga cggcttccgc
     3421 cgggcggcct acgtgcccgc ctccggatgg tcctcgcagg ccctcgagta cgaggtgcgc
     3481 atccgcggcc tggacgacgg ggtgcacgtg ttccggctcg cggggctcac ggtggacggc
     3541 cggatctcgt attcggacga gattctcgtg accctcggca agccgtccgg ctacgcgctg
     3601 gcgctcgacg ccccgtaccc gaaccccgcg accgaccggg cgacggtcgc cgtctcgatg
     3661 gaccgctacg agaacgcccg gatttccgtg ttcgacctcc tgggccggga ggtggccgtc
     3721 ctccatgatg gcatgctcca gcccgggatc gagtcgcggt tcgagttcga gccctcggat
     3781 tccctgccgt cgggggtcta cgtcgtacgc gcgtcggccg gcgagctgat gcgctcccag
     3841 ctgctcaccg tgatgcggta ggtctcccgc cgggcgaccg tcccgacgat cgcaacgcct
     3901 tgggccgccg cgatccggcc ggcccg
//
LOCUS       DBNHQZ010000251         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1157647, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000251 DBNHQZ010000000
VERSION     DBNHQZ010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1157647"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16YU_06735"
     CDS             complement(<1..222)
                     /locus_tag="AC16YU_06735"
                     /inference="COORDINATES: protein motif:HMM:NF046799.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6064956.1"
                     /translation="MTRVSARFLLPALLLFVLGLAVGARFSGVFRSPDSLEQLRKLED
                     AYMLIDRKYVEDVPAERVVERAIEAMLDEL"
     gene            415..1014
                     /locus_tag="AC16YU_06740"
     CDS             415..1014
                     /locus_tag="AC16YU_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6064957.1"
                     /translation="MRSKGMLVVIFVILLVGFAGCAGCGAYNGLVASDESVTQAWANV
                     ETQYQRRSDLIPNLVSTVQGAADFESGTLEAVTSARARATSITLQADDLSDPARLQSF
                     MEAQSALGSSLGRLLAVAENYPELRATEAFRDLQAQLEGTENRINQARRDYNAAVATF
                     NTSVRRFPTNIVASLTGFERRVPFEADPGTEKAPVVEFN"
     gene            1022..1810
                     /locus_tag="AC16YU_06745"
     CDS             1022..1810
                     /locus_tag="AC16YU_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR6064958.1"
                     /translation="MIQRLAALLLVLATAAPAPAQTPNVLAPSGKWVTDAAEMLSSAE
                     ENLLSQRLSGFADTTSTQMIVVTIPTLDGSAPVDYAVALGRSWGVGQRGRDNGIVVLV
                     ARDEREVFIATGFGLEGAVPDAVASRIVRRLVVPRFREGRYYDGLSDAVDALMLATAG
                     EFDAIAPDDGPDGLPVIALAFIVLVILVIVISSARQGGSGGGSGGTRYRSRSGPVIIW
                     GNPGFGGRHGGFGGGFGGGFGGGGFGGFGGGGGSFGGGGAGGSW"
     gene            1819..2148
                     /locus_tag="AC16YU_06750"
     CDS             1819..2148
                     /locus_tag="AC16YU_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6064959.1"
                     /translation="MRRLDTLLQYHSEDPDDGFVRFAVASEYLKEGKLAEALSWFEGL
                     AADQPDYVGTYYHLGKLYERLGRTGDAIETYEKGMAESARQKEFHAWAELESALSEAR
                     GNGEEDA"
     gene            2145..>2385
                     /locus_tag="AC16YU_06755"
     CDS             2145..>2385
                     /locus_tag="AC16YU_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6064960.1"
                     /translation="MSTLRSLATSALAVVAAGMLTLATTGPVRAQTPDSTHVVQAGET
                     LYAISRRYGMTIAELQRLNGLEGTNIRVGQRLRIVA"
BASE COUNT          349 a          826 c          842 g          368 t
ORIGIN      
        1 gagctcgtcg agcatggcct cgatggcacg ctcgacgacg cgctcggccg gcacgtcctc
       61 gacgtatttc cggtcgataa gcatgtacgc atcctcgagc ttgcgcagct gctcgagcga
      121 atccggagac cggaaaacgc cgctgaaacg ggcacccacc gccagtccga gcacgaagag
      181 gagcagggcc gggaggagga atcgggcgga gacgcgggtc atgtacgcgg tcgatggtcg
      241 ggcgattcgg gacggaatga ttcgccggtc ggcctccatt tgtttcgcgg ggccgtcgga
      301 tcgccccgta cgcggcaccg ggcgcggttc cggcagaaac cctccggaga gcggctcgta
      361 tgccgtcgcc gggccgtgtg ccctccgcct ccagccacca tccggtctcc cgccatgcgc
      421 agcaaaggga tgctcgtcgt cattttcgtg atcctgctcg tcggtttcgc cgggtgtgcc
      481 ggatgcgggg cctacaacgg cctcgtggcc tcggacgaat cggtgacgca ggcctgggcc
      541 aacgtcgaga cccaatacca gcggcgctcc gacctcatcc cgaacctggt ttcgaccgtg
      601 cagggcgcgg ccgatttcga gtccgggacc ctcgaggccg tgaccagcgc gcgggcccgg
      661 gccacgtcga tcacgctcca ggcggacgac ctgtccgatc cggcccggct gcagtccttc
      721 atggaggccc agtcggctct cggctcgtcg ctcggtcgcc tgctcgccgt tgccgagaat
      781 tatcccgagc tccgggccac ggaggccttc cgcgacctgc aggcgcagct cgaggggacg
      841 gaaaaccgca tcaaccaggc ccggcgcgac tacaacgccg cggtcgcgac gttcaatacg
      901 tcggtccgcc gcttcccgac gaacatcgtc gcgtcgctga ccggcttcga gcggcgggtc
      961 ccgttcgagg ccgatcccgg cacggagaag gctcctgtcg tcgaattcaa ctgaccgccc
     1021 cgtgatccag cggctcgccg ccctcctcct ggtcctggcc accgccgcac cggccccggc
     1081 gcagaccccg aacgtgcttg cgccgtcggg gaagtgggtg accgacgcgg cggagatgct
     1141 ctcgtccgcg gaggagaacc tcctctcgca gcggctctcc ggcttcgccg acaccacgtc
     1201 gacccagatg atcgtcgtca cgatcccgac gctcgacggg tcggcgccgg tcgattacgc
     1261 cgtggcgctc ggccgctcct ggggcgtggg ccagcggggg cgggacaacg ggatcgtggt
     1321 cctcgtcgcc cgcgacgagc gcgaggtgtt catcgcgacg ggattcggcc tcgagggcgc
     1381 cgtgcccgat gccgtcgcga gccgcatcgt gcgccgcctg gtggtgcccc gcttccgcga
     1441 gggccgctac tatgacggcc tctcggacgc cgtggacgcg ctgatgctcg ccacggcggg
     1501 cgagttcgac gccatcgcac cggatgacgg tcccgacggc ctgccggtca tcgccctcgc
     1561 cttcatcgtt ctcgtgatcc tggtgatcgt catctcgtcc gcccggcagg gaggatcggg
     1621 tggaggctcc ggcggaaccc gctaccgctc ccgcagcggg ccggtcatca tctggggcaa
     1681 cccgggcttc ggaggtcgtc acggcgggtt cgggggtggc ttcggcggtg ggtttggcgg
     1741 gggcggcttc ggcggtttcg gcggcggcgg cggctcgttc gggggaggag gcgccggcgg
     1801 aagctggtga cagcagccat gagacgactc gacacgctcc tgcagtacca ttcggaggat
     1861 ccggacgacg gcttcgtccg cttcgccgtg gcgtcggagt acctgaagga gggcaagctg
     1921 gccgaggcgc tctcctggtt cgaagggctc gcggccgacc agccggatta cgtcgggacg
     1981 tactatcatc tggggaagct gtacgagcgt ctcggccgga ccggggacgc gatcgagacc
     2041 tacgagaagg gcatggccga gagcgcccgt cagaaggaat tccacgcgtg ggccgagctc
     2101 gagagcgcgc tctccgaggc ccgcggaaac ggggaggagg acgcatgagc acgcttcgct
     2161 ccctcgcaac cagcgccctg gccgtcgtgg ccgccggcat gcttaccctg gccacgaccg
     2221 gtcccgtgcg ggcgcagacc cccgacagca cgcacgtcgt gcaggccggg gagacgctct
     2281 acgccatctc gcgccggtac ggcatgacca tcgccgaact gcagcggctc aacgggctcg
     2341 agggcacgaa catccgggtg gggcagcgcc tgcgcatcgt cgcgg
//
LOCUS       DBNHQZ010000252         8415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2514591, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000252 DBNHQZ010000000
VERSION     DBNHQZ010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2514591"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16YU_06760"
     CDS             complement(<1..638)
                     /locus_tag="AC16YU_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6064961.1"
                     /translation="MTHRITLIPGDGIGPEVTEATIRVLEATGCRFEWDRHDEVGTAA
                     VERLGTPLPDSILDAVRATGVALKGPVTTPVGEGFQSVNVRLRQKLDLYANVRPCRSL
                     PGIETPFPDVNLVIFRENTEGLYSGLESYDERHDIADSVARISRKGSERIIRFAFDYA
                     RRHGCGPLTVVHKANILKLTSGMFLSIARKYALEYPDVEFNDRIIDNMCMQLV"
     gene            complement(765..1220)
                     /locus_tag="AC16YU_06765"
     CDS             complement(765..1220)
                     /locus_tag="AC16YU_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6064962.1"
                     /translation="MSSQHFKKKTQTKQDIPTASLPDIIFMLLIFFMVSTVLRETSVQ
                     VRTLLPQAEALTKIDQKRLVSYVWIGPRKLEGNRIGDTAVQIDDAMVEDVTNVRTIMY
                     RKLVEQPRLIVSLRVDQQSEMGIVLDVQQELREAGTLRINYSTKRLVTI"
     gene            complement(1259..1819)
                     /locus_tag="AC16YU_06770"
     CDS             complement(1259..1819)
                     /locus_tag="AC16YU_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6064963.1"
                     /translation="MAGLLRKRKRNEAEIPTASMADIAFLLLIFFLVTTTIDVDTGIG
                     MVLPPKLEEDVEPPPIRERNVLKILVNEQGMVLLEDMPSSVSLIREEVKKHVLNNGVD
                     INYAESPGRALVSIKTARQTPYNAYIEVLDEVWMAYFEMWDSEARRLGYADYEAYNAA
                     IEASGAENEIRENIKAQISIAEPDKA"
     gene            complement(1847..2122)
                     /locus_tag="AC16YU_06775"
     CDS             complement(1847..2122)
                     /locus_tag="AC16YU_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064964.1"
                     /translation="MESLIPIAVWLVLVLIGLSLVAIAVFAVRNLTYGKVNALTIGLT
                     FIPIALLVVLGFATADWWYAGVIVVIVMLVITSITLLLSGIRGLIGL"
     gene            complement(2162..2851)
                     /locus_tag="AC16YU_06780"
     CDS             complement(2162..2851)
                     /locus_tag="AC16YU_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6064965.1"
                     /translation="MKLFSLLLMLPQEAAAEDGFVNVLVARFNEGGDYMWPILISLIV
                     GLAIAFERIITLNRADINTRKFIVKVKQSLESGGIAAAEEVCASTRGPVASVFQAGLL
                     RHDEGIEAVEKAVVSYGSIEMSFLERGLVWLSLFISLAPMFGFLGTVVGMVEAFDAIE
                     AAGDISPSLVAGGIKVALLTTVFGLISAIILQFFYNYITSKIDRIVIDMEEASIELID
                     SLVLLQAGRRV"
     gene            complement(3054..4727)
                     /locus_tag="AC16YU_06785"
     CDS             complement(3054..4727)
                     /locus_tag="AC16YU_06785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6064966.1"
                     /translation="MPRTKLAIYALLLLAAGVLLGARLGGSFFEPDVSKALRKVEDVF
                     VMIAQRYVDEVDADRLAEDAIEGMLGALDPHSVYVDERRTRLETEQFNAEFEGIGVAY
                     AFIDGEGGLDTLVVVEVLPGGPSESIGILSGDRIVSIDDSTAIGIENDDVHRRLRGPR
                     GTHVRVTLVRPGYDRPLDVEITRDRVPIVTVDAAWLLEDSTGVIRLNRFARTTPQEFR
                     QALRDLAGRGMTRLVLDLRGNAGGLMNAAVPVAGEFLERGQVIVSQRGRADDANEVYR
                     ARGEGLWRTGPLIVLVDAGSASASEIVAGAIQDHDRGLIVGRRTFGKGLVQNQVTLAD
                     GSAVRLTVARFYTPSGRLIQTRYENGDRESYYEEKHARLAGEGMISVSELAAEAPDSL
                     RFTTDGGRTVLAGGGIVPDYVTRDSTAAYLRAILVRGLEQDFVRAWIDRDGESLRARF
                     GDDLDRYVREFQVTDADLARFRTHAEARGLHVGRAPADDTSGDEWITRERYEEAEPDL
                     RLLLKGRIARRLFDRAAWYPVFAPADRTLQQARTLWAEAARLSPGMGEG"
     gene            4877..6718
                     /locus_tag="AC16YU_06790"
     CDS             4877..6718
                     /locus_tag="AC16YU_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6064967.1"
                     /translation="MIRVLRIAAALLLALAASGVVVWYLAFRVGNRQPERLAVEGLRA
                     GASITWHEGGEAAVRLEDIRDLERVTGAVHALGRPWLLLLLRQAALGRLAEWYGREAL
                     PADHHVHRLGIPATARAAFGRLDQPGQERLRAYADGVNSALGDPRMRLAPAVAALDLD
                     PEPWEPWHALAVERLLAWLAATPATGSVGPGPAVRGFLEARDALGELLGLGGLDHGAA
                     WQMANQPPGIFVRLVYGTSGIPPICVFSVQSPEGRLDLVTWLGMPTAWAGRSDERSWA
                     VLPSGHAVVDSGAVAAEPVYVPLRLRDGSRDVAVRRTDGRHVAVTGDLRVLWSGIAPV
                     SDLGAIADLAAGRPADFRLVDGLAMTTDHSRPGPARTFGSRGTAHGKVVLVSSAAEAR
                     NLAESLDAVQELGAGRVLDDTGTAWAAAASDLAEELMAADSAATSAEREALDFLSTWD
                     GSFDGPSIGAALFDAMRQAPGSTGAQAFLAGVDSLRRLFGTNARSWRWERTGPGLRYP
                     GYLDSSPYPDLPKLPGRYAPVELPRHGHPTTASWGSALPYVNPPAPAAWEAFIEDRDG
                     GTVLYRRPFVPYDRLLGRFSTTPGERGLTALAGRELTGERTELVPNR"
     gene            6723..7625
                     /locus_tag="AC16YU_06795"
     CDS             6723..7625
                     /locus_tag="AC16YU_06795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR6064968.1"
                     /translation="MAHVAGIDAGGTRCRVLVAPIDGTGTGRTVEGNAANVQILGIDG
                     VAERLAELVREAHPGPLAAVVAGVAGAGREGDRKTLTEALSARLGTPADRVRILADVD
                     LALDAAFGPDQPGMIVVAGTGSIACAREASGRTVRAGGWGRVLGDEGGGHDLGVRGLS
                     AVADAMDGGPATALRDAASRAWDADDAASLIAAVYRDRRNPSDFAPEVLRAATAGDAV
                     ALAIARAAASELALRASWVAGRCAVEPPRFALWGGLTRNPLYRDLLADEIGRDLAGWT
                     RIEMTERPVDAALRLARTLALEAP"
     gene            complement(7679..>8415)
                     /locus_tag="AC16YU_06800"
     CDS             complement(7679..>8415)
                     /locus_tag="AC16YU_06800"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR6064969.1"
                     /translation="IYAAIRFGTVLENVVFDPETREVDFADTSITENTRAAYPIEYID
                     HAKIPGIGGHPAHIIFLTYDAFGVLPPVSRLSPDQAMYHFISGYTAKVAGTEQGVTEP
                     EATFSACFGAAFLVMHPARYAELLAEKMRRHGAEAWLVNTGMTGGSYGTGRRMSLKHT
                     RAIIDAIHAGLLDSVDTEEDPVFGLAVPTEVPGVPSSVLVPRRAWADPAAYDATANRL
                     ARLFAENFRKYADGAAPEVLAAGPKV"
BASE COUNT         1268 a         2931 c         2920 g         1296 t
ORIGIN      
        1 acgagctgca tgcacatgtt gtcgatgatg cggtcgttga actcgacgtc gggatactcg
       61 agggcgtact tccgagcgat gctcaggaac atgcccgacg tgagcttcag gatgttggcc
      121 ttgtgcacca ccgtcagcgg tccgcacccg tggcgccggg cgtagtcgaa ggcgaaccgg
      181 atgatgcgct cggagccctt ccgggagatg cgggcgaccg agtcggcgat gtcgtggcgc
      241 tcgtcgtacg actcgagccc cgagtacagg ccctccgtgt tctcgcggaa gatgaccagg
      301 ttcacgtcgg ggaacggcgt ttcgatcccg ggcagggacc ggcacggtcg aacgttcgca
      361 tacaggtcca gcttctggcg gagcctgacg ttcacgctct ggaagccctc gcccaccggg
      421 gtggtgaccg gacccttgag cgcgacgccc gtcgcgcgca cggcgtccag gatcgagtcc
      481 gggagcggcg tgccgagccg ctcgacggcg gccgtgccca cctcgtcgtg gcggtcccac
      541 tcgaaccggc agccggtggc ctcgagaacg cggatcgtgg cctcggtgac ttcggggccg
      601 atcccgtcgc cggggataag ggtgatgcga tgcgtcatgg acgaatgcgg gcgcgttggt
      661 gcctttcggc ggggaccatc cggtacgcac gcaccgaatc tacgcaaaga aggagccccg
      721 cacgggcggg actccttcga ttccgttccg gtccgggacg gacgtcagat cgtgaccagg
      781 cgcttggtcg agtaattgat gcgcagggtg ccggcctcgc gcagctcctg ctgcacgtcg
      841 agcacgatgc ccatctcgga ctgctggtcc acgcgcagcg aaacgatcag gcggggctgc
      901 tcgacgagct tccggtacat gatcgtccgt acgttggtga cgtcctccac catcgcgtcg
      961 tcgatctgca ccgcagtatc gccgatccgg ttaccctcga gcttgcgcgg cccgatccac
     1021 acgtacgaga ccaggcgctt ctggtcgatc ttcgtgaggg cctcggcctg gggcaggagc
     1081 gtccgtacct ggaccgacgt ttcccggagc accgtgctga ccatgaagaa gatcagcagc
     1141 atgaagatga tgtccggaag cgaagcggta ggaatgtcct gcttcgtctg ggtcttcttt
     1201 ttgaaatgct ggctggacat ccgagtcgtg ggtcaagcgg tgaaactgcc gttggggatc
     1261 acgccttgtc gggttcggcg atcgagatct gggccttgat gttctcgcgg atctcgttct
     1321 ccgcgccgga cgcctcgatc gcggcattgt acgcttcgta gtctgcgtac cccagccgcc
     1381 gggcctccga gtcccacatc tcgaaatagg ccatccacac ctcgtcgagg acctcgatgt
     1441 acgcgttgta cggggtctgg cgcgccgtct tgatcgagac gagcgcacgg cccggcgact
     1501 ccgcgtagtt gatgtcgacg ccgttgttga gcacgtgctt cttcacctct tcccggatca
     1561 ggctcaccga cgagggcatg tcctcgagaa gcaccatgcc ctgttcgttg acgaggatct
     1621 tgagcacatt gcgctcgcgg atcggcggcg gctcgacgtc ctcctcgagc ttgggcggca
     1681 gcaccattcc gatcccggtg tcgacgtcga tggtggtcgt caccaggaag aagatcagga
     1741 gcaggaacgc gatgtcggcc atcgacgcgg tcggaatctc cgcctcgttg cgcttgcgct
     1801 ttctgagcag accagccatg tcggttcgta tgccggaaaa ggatggttac aggccgatga
     1861 gcccgcggat cccggacagg agcagcgtga tcgacgtgat cacgagcatc acgatcacga
     1921 cgatgacgcc cgcgtaccac cagtcggccg tggcgaagcc cagcaccacg agcagcgcga
     1981 tcggaatgaa cgtcaggccg atggtcagtg cgttcacctt gccatacgtc aggttgcgca
     2041 cggcgaaaac cgcgatggcg accaggctga gtccgatcag gaccagcacc agccagacgg
     2101 cgatgggaat caagctttcc atgggagtac ctccttgcag gaatgagtgg acggagcccg
     2161 gtcagaccct ccggcccgcc tgcaggagca ccagggaatc gatcagctcg atcgacgcct
     2221 cttccatgtc gatcacgatg cgatcgatct tggatgttat gtagttgtag aagaactgga
     2281 ggatgatggc cgagatcagg ccgaacacgg tcgtgagcag ggccaccttg ataccgccgg
     2341 ccacgaggga cggcgagatg tcgccggccg cctcgatggc gtcgaacgcc tcgaccatgc
     2401 cgaccacggt cccgaggaat ccgaacatcg gggcgagcga gatgaagagg gagagccaca
     2461 ccaggcctcg ctcgaggaag ctcatctcga tggagccgta ggacacgacc gccttctcga
     2521 cggcctcgat gccctcgtca tgacgcagca gccccgcctg gaagaccgac gcgaccgggc
     2581 cgcgcgtgct cgcgcagacc tcctcggccg ccgcgatgcc gcccgactcg agcgactgct
     2641 tcaccttgac gatgaacttg cgggtgttga tgtcggcccg gttcagcgtg atgatgcgtt
     2701 cgaaggcaat ggcgagaccg acgatcagcg aaatgaggat cggccacatg tagtcgccgc
     2761 cctcgttgaa ccgagccacc agcacattca cgaaaccatc ctcggcagcc gcctcctgcg
     2821 gcagcatgag aagcaggctg aagagcttca tggataccat tcctccgggt aggggtgtcg
     2881 tttgaagtag aggggcgaat cggaaaccgc ggagaaccgc gagcgggcac cctcggacat
     2941 gcgtacgctt tcgatgtcga cgacgtcgtc gagcacgtga gacgaggcga aatgatagcc
     3001 cacgcccggc cgattgtcaa tcaggaagcc ccctccaggg gcgcgccggg gcgtcacccc
     3061 tcgcccattc ccggggacag gcgggcggcc tcggcccaca gggtgcgggc ctgctgcagc
     3121 gtgcggtcgg ccggcgcgaa gacggggtac caagcggccc ggtcgaagag ccgccgggcg
     3181 atccggccct tgagaaggag ccgcaggtcc ggctccgcct cctcgtagcg ttcgcgcgtt
     3241 atccactcgt cgccggaggt gtcgtccgcg ggcgcccggc ccacgtgcag tccacgcgcc
     3301 tccgcatggg tgcggaagcg cgcgaggtcg gcgtcggtga cctggaactc ccggacgtac
     3361 cggtccaggt cgtctccgaa gcgcgcgcgc aggctctccc cgtcgcggtc gatccacgcg
     3421 cggacgaagt cctgctccag gccccggacg agtatcgccc gcaggtatgc ggccgtcgaa
     3481 tcacgggtca cgtagtcggg cacgatgcct ccaccggcga ggacggtgcg gccgccgtcg
     3541 gtggtgaacc ggagcgagtc gggcgcctcc gcggcgagct ccgagacgct gatcatgcct
     3601 tcccccgcga gccgcgcgtg cttctcctcg taatagcttt cgcgatcccc gttctcgtac
     3661 cgcgtctgga tgagccggcc gctcggcgtg tagaacctcg cgacggtaag ccggacggcc
     3721 gatccgtcgg cgagcgtgac ctggttctgg accaggccct tgccgaacgt ccgtcgtccg
     3781 acgatcagcc cgcggtcgtg atcctggatg gcgcccgcca cgatctccga ggcggaagcg
     3841 gacccggcgt ccacgagcac gatcagcggg ccggtgcgcc agagcccctc gccgcgcgcg
     3901 cggtacacct cgttggcgtc gtcggcgcgg ccccgctgcg agacgattac ctggccgcgc
     3961 tccaggaact cgccggcgac cggaacggcc gcgttcatca gcccgcccgc attgccgcga
     4021 aggtccagga ccaggcgcgt catgccgcgg cccgcgaggt cccgcagggc ctgccggaat
     4081 tcctggggcg tggtgcgcgc gaaccggttc agccggatga ctcccgtcga atcttccagc
     4141 agccacgccg cgtccacggt cacgatcgga acccggtcgc gtgtgatctc gacgtcgagg
     4201 ggccggtcgt acccgggacg gaccagggtc acgcggacgt gcgtgccccg cggtccgcgg
     4261 agacgccggt gcacgtcgtc gttctcgatg ccgatcgcgg tggagtcgtc gatcgacacg
     4321 atccggtcgc ccgagagaat cccgatcgac tcactggggc cgccgggcag cacctcgacc
     4381 acgaccagcg tgtccaggcc gccctccccg tcgatgaagg cgtacgcgac gccgatgccc
     4441 tcgaactcgg cgttgaactg ctcggtctcg agtcgggtgc gccgctcgtc cacgtagacc
     4501 gagtgcgggt cgagcgcgcc cagcatgccc tcgatcgcgt cctcggccag ccggtcggca
     4561 tccacctcgt ccacgtaccg ctgggcgatc atcacgaaga cgtcttccac cttgcggagc
     4621 gccttcgaga cgtcgggctc gaagaacgat ccaccgaggc gtgcaccgag cagcacgccc
     4681 gcggcgagga gcagaagagc gtagattgcg agtttcgtgc ggggcatcgc ggacggggat
     4741 ggtgcgtgga actccccccg gcgcacgcgg cggccgcggg gcccgcagta acgggccggg
     4801 accgacccag gatcccgcca ccgggcgttg ggccgagcgg gccgggaccg tagtttcggg
     4861 gccacccacg atcgccgtga tccgcgtcct ccgcatagcc gccgccctgc tcctcgccct
     4921 agcggcgagt ggcgtcgtgg tatggtacct cgccttccgg gtcggaaacc ggcaaccgga
     4981 gcggctggcc gtcgaaggcc ttcgcgcggg agcatcgatc acgtggcacg aggggggcga
     5041 ggccgcggtc cggctggagg acatccggga cctggagcgc gtcaccggag cggtgcatgc
     5101 tctcggccgg ccctggctcc tcctcctgct tcgccaggcg gcgctcggcc ggcttgcgga
     5161 gtggtacggc cgcgaggcgc tgcccgcgga ccaccacgtg caccggctcg ggatacccgc
     5221 cacggcccgt gcggcgttcg gacggctgga ccagcccggg caggagcgcc ttcgcgcgta
     5281 tgccgacggc gtcaactcgg cactcggcga cccgcgcatg aggctggcac cggcggtcgc
     5341 cgcgcttgat ctggatcccg aaccgtggga gccctggcat gccctggccg tggagcgcct
     5401 cttggcctgg ctcgccgcca caccggccac cggatccgtc ggtcccgggc ccgcggtccg
     5461 gggcttcctc gaggcccggg acgcgctcgg agagctgctc gggctgggcg gcctggatca
     5521 tggcgccgcg tggcagatgg ccaaccagcc tccgggcatt ttcgtgcgcc tcgtatacgg
     5581 cacgtcggga attccgccaa tctgcgtgtt ttcggtgcag tcgccggaag ggcggctgga
     5641 ccttgtgacc tggctgggca tgccgacggc ctgggccggt cgcagcgacg agcgctcatg
     5701 ggcggtgctc ccgtccggcc acgccgtcgt ggactcgggg gccgtcgcgg ccgagcccgt
     5761 ctacgttccc cttcggctcc gtgacggaag ccgggacgtg gccgtgcgca ggacggacgg
     5821 gcggcacgtc gcggtcactg gcgatctccg cgtgctctgg tcaggcatcg cgcccgtctc
     5881 ggacctgggg gcgatcgccg acctggccgc gggccggccc gctgatttcc gcctggtgga
     5941 cggcctggcc atgacgaccg atcactcgcg tcccggtccc gcacgcacct tcggaagccg
     6001 cggcaccgcg cacggcaagg tggtactcgt ttcctcggcg gccgaggcgc ggaacctggc
     6061 cgagagcctc gatgcggtac aggaactggg ggcgggccgc gtgctcgacg acaccggcac
     6121 cgcgtgggcg gcggccgcgt cggacctggc cgaagagctc atggcggccg actctgccgc
     6181 tacgtccgcc gagcgggagg ccctggattt cctgtccacg tgggacggct ccttcgacgg
     6241 cccgtccatc ggcgccgctc tcttcgacgc catgaggcag gctcccggat cgaccggcgc
     6301 gcaggcattc cttgcgggag tagacagcct gcggcgcctg ttcggcacga acgcgcggtc
     6361 gtggcgctgg gagcgaaccg gcccggggct ccgctatccc ggctacctgg actccagccc
     6421 ctaccccgac cttccgaagc ttccgggccg ctacgcgccc gtcgagctcc cgcgacacgg
     6481 acacccgacc acggcgtcct ggggaagtgc ccttccgtac gtgaacccgc ccgccccggc
     6541 ggcgtgggaa gccttcatcg aggatcgcga cggcgggacg gtgctctacc ggcggccgtt
     6601 cgtaccgtac gaccggctgc tcgggcgctt ctcgaccacg cccggcgagc gcgggctcac
     6661 cgcgctcgcc gggcgcgagc tcaccggtga gcgcaccgag ctcgtcccca accgatagcc
     6721 ccatggccca cgttgccgga atcgacgcag gaggaacccg ctgtcgcgtg ctcgtcgcgc
     6781 cgatcgacgg gacgggcacc ggacgaaccg tcgagggaaa cgccgcgaac gtgcagatac
     6841 tgggcatcga cggggtcgcc gaacggctcg ccgaactggt gcgggaggcg caccccggcc
     6901 ccctggccgc cgtggttgcc ggcgtggccg gcgccgggcg ggagggcgac cggaaaaccc
     6961 tcacggaggc gctctcagcg cggctcggca cgcccgcgga ccgcgtccgc atcctggccg
     7021 acgtggacct ggcgctcgac gccgccttcg gcccggacca gcccggcatg atcgtggtcg
     7081 cgggcaccgg ctcgatcgcc tgcgcacggg aggcgtccgg ccgcacggta cgggccggcg
     7141 gatggggacg ggtgctgggc gacgaagggg gaggccacga cctgggcgtg cgtggtctct
     7201 cggccgtcgc ggacgcgatg gacggagggc cggcgaccgc gctgcgcgac gccgcctcgc
     7261 gcgcctggga cgccgacgac gccgcttcgc tcatcgccgc cgtgtacagg gaccgccgca
     7321 acccgtccga tttcgccccg gaagtcctcc gcgccgccac cgcgggcgac gcggtcgccc
     7381 tcgcgatcgc ccgcgccgcc gcgtccgagc tcgcgctccg ggcctcgtgg gtggcagggc
     7441 gctgcgccgt cgagccgcca cgcttcgcgc tgtggggcgg gctcacccgg aacccgctct
     7501 accgcgacct gctcgcggac gagatcggcc gggacctcgc cgggtggacc cggatcgaga
     7561 tgaccgagcg gcccgtggac gccgcgctcc gcctggcccg cacgctggcg ctcgaggcgc
     7621 cctgagcggc cggccccgac tccgacgacc tcggcgccca ggcgcgccgc cgccccgcct
     7681 acaccttcgg ccccgccgca agcacttccg gtgccgcgcc gtcggcgtat ttccggaagt
     7741 tctcggcaaa gagccgagcc agccggttcg ccgtcgcgtc gtaggcggct ggatcggccc
     7801 aggcgcgtct cggcaccagc accgacgacg gaacccccgg cacctcggtc gggaccgcga
     7861 gcccgaacac agggtcctcc tcggtgtcca cactgtcgag cagcccggcg tggatggcgt
     7921 cgatgatcgc ccgggtgtgc ttcaggctca tccgccggcc ggtgccgtac gagccgccgg
     7981 tcatgcccgt gttcacgagc caggcctcgg ccccgtgacg gcgcatcttt tcggcgagaa
     8041 gctcggcgta gcgcgccggg tgcatgacca ggaacgccgc cccgaagcac gccgagaacg
     8101 tggcctcggg ttcggtcacg ccctgttccg tacccgccac cttcgccgtg tacccgctga
     8161 tgaagtggta catcgcctgg tcgggcgaca gccgcgagac cgggggcagc accccgaacg
     8221 cgtcgtacgt caggaagatg atgtgcgcgg ggtgcccgcc gatgcccggg atcttcgcgt
     8281 ggtcgatgta ctcgatcggg taggccgcgc gcgtgttctc ggtgatggac gtgtcggcga
     8341 aatccacctc gcgcgtctcc gggtcgaaca ccacgttctc gagcaccgtc ccgaaccgga
     8401 tcgccgcgta gatct
//
LOCUS       DBNHQZ010000253         9770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1158605, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000253 DBNHQZ010000000
VERSION     DBNHQZ010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1158605"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2539)
                     /locus_tag="AC16YU_06805"
     CDS             complement(<1..2539)
                     /locus_tag="AC16YU_06805"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6064970.1"
                     /translation="MTGLHLAQYRIDAELGRGGMGIVYRATDTKLNRTVALKVLPAAA
                     LSSEDDRARFVREAQSAAQLHHPNIATVFGIDEAVPVEEGVAPQPGTESRLFIAMEYI
                     EGETLAEMVKRGPMKLHDAVNVAGQVAEALKEAHAKQIVHRDVKSANVMLTQAGVAKV
                     LDFGLAKTNQSTMLTRMGSTLGTVAYMSPEQARGHEVDGRTDLYSLGTMLYEMVAGKL
                     PFGGDYEQAVLYGILNEPPEPLTAVRTGVPMELERIVAKLLSKEAEYRYQTAADLLAD
                     LKSLSLGGSGVTRRSMPAMSTAGMPAASAPGRKLPLWAYGAIIGALLLGAAGSWLATR
                     SPEPAPPPTRTVPLAVEDVASARVMDISPDGGLVAVKTQGGQGDWSIVLYDTGIGEWT
                     GPISLEVTDNLEFSPSGEWLLASNIDINGPLVRYRLSDDRPQTIPLRGAAATWLNDEE
                     ILYTPSDLGLWRAEVDGQNARKVVSADSIPPVDVIWYAHPLPGERQVVATLGAFNGVA
                     LGIVDLESGSLRRLADDICCARYLPSGHLLYTQQPMSNGTFMLRPFDAARARFTGVEV
                     PAGISSNPFSEIGVGADGTLAYALTTASRSEFVYYWIGTDGRLVGEQDEFSVGPMILP
                     YGGGSSGDWGRSGVEHAEISRDGDRFVAARFADGEYRVFVNDLARRSSQRVSFDGPSG
                     WPTWSPDGRWIYYVGRRAGKDVILRRRADLSAAEEVVLVGKGTLADPEITPDGRRLLY
                     YTTDDEVGRIVDLADTTLQHVFSETTAFDPMVSPDGRYVAFESQGLDVRDLDGTALYQ
                     VAPEFGDAWDPSWSADGKYLYYRDTGTLYRVPVETTPVFSQGPREIVA"
     gene            complement(2628..5246)
                     /locus_tag="AC16YU_06810"
     CDS             complement(2628..5246)
                     /locus_tag="AC16YU_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064971.1"
                     /translation="MSPRPPRFIARLAERWLLPEYESLYGDLREDHLDDVRRFGRAPA
                     GLVYASRLLYLVLYLRLRHARRSNIMLRVNLLVALRNLRKSLGYSAINIIGLAVSMSV
                     GLLVITLLREQLSYDRFHANADRIWRITSEVEEPFGRFPMATSAGPLGPALLEDVPSV
                     ERATRLRRMGGSASYGDVTLPLGGLYAEPSFFELFDFPMVRGNPATALSAPYSVVLTR
                     SSAERYFGGADPVGKVLQRGDLGGFTVTGVLEDPPTNTHLAFEALASFSTLVALSGTD
                     DPYSIEDWTVSSTLYTYLLLAPGARTEDIVARAQSLVDAHEWNSERPRPTIGMQALTD
                     INLGEDLSNQLGPVMPKDASYFLLFFGAILLIIAVFNYVNLTIARAIRRAREIGIRKV
                     MGAARAQIVAQFALEAVVVALLALVVAAAMLVWLLPAFNSLSGFRDALDLGSITVDPF
                     RDGLIYLLYIGFAVAVGMVAGLVPAFRMSSIAPSPVLRGLAGAAGRREGLGRRGLIVF
                     QFAVAIVGVVTALLVHRQMSMVLQADYGFGEDRLLSVDLQDAPFGAVAHEFSSLAGVE
                     GVAATSTMPSGESKVWTSIRGENGEDDLLLQFFSVDAEFPRQLHLEVVEGRGLDGVVP
                     GLDPEPVWLTVAATRALGYEQPGDAVGAALTVSPTWEAHPGTVVGVLADFYARGYERG
                     YTPAMLRVNPEGYQRAVVRLAPGDVQTTVGALEDAFEKVAPTAPFVYRFYDEEIASQY
                     TFVRDLVRFLGLLAAFVILIACLGLLGAASHSAQRRFREVGIRKVLGADVRGTVALLS
                     RDYVRMIAVAVLVAGPLSYLAGRALLEKFPNHVEFDAWLFAMGILPIVALALVTVGTQ
                     TVRAALTNPVETLRQE"
     gene            complement(5268..5600)
                     /locus_tag="AC16YU_06815"
     CDS             complement(5268..5600)
                     /locus_tag="AC16YU_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR6064972.1"
                     /translation="MDHLTRIEELLLLTVWKLGDEAYGLAVRRALSQLLRRRVSVGAV
                     YVPLERLAGHGLLESWEGEPTEERGGRRKRFYRLTGEGVRALEAVRLLEERAWSGLPR
                     LRAGLSET"
     gene            complement(5744..6691)
                     /locus_tag="AC16YU_06820"
     CDS             complement(5744..6691)
                     /locus_tag="AC16YU_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIR6064973.1"
                     /translation="MEKKLIAVLGATGAQGGGLARAILDDERGEFALRAITRNPDSEK
                     ARALAARGAQVVRADLDDVDSLAAAFDGCHGAFCVTNFWEHLSPEKDTQQAQNLAIAA
                     KRAGIRHAVWSTLEDTRKWVPLDDDRMPTLQGKYKVPHFDAKGQADRFFTEEGVPTTF
                     FSASFYWDNLIHFGMGPRRGEDGRLVFALPMGDARLPGIAAEDIGKCALGVFKAGEDF
                     IGRRVGVAGGRLTGAQMAEAMAAALDEPVDYYAVPFDTYRSFGFPGAEDLGNMFQHHH
                     DFNEDFLALRDVDLSRTLNPELQSFADWLESNAEQIKPD"
     gene            6871..7506
                     /gene="trhA"
                     /locus_tag="AC16YU_06825"
     CDS             6871..7506
                     /gene="trhA"
                     /locus_tag="AC16YU_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR6064974.1"
                     /translation="MAHRSQSRGEEWANALSHGAGALGAAVALPVLVIEALRSGSAMA
                     VVGAVAFAASALFLYAASTAYHAAPTGRAKRVLRHLDHVGIFVLIAGSYTPFMLGPLR
                     GPWGWSLLTAVWSLALLGIVWKVHPRLRRERAPVWLYLLMGWLVVVAVRPLWIHVSGG
                     ALAWMALGGLAYTGGVLFYATDRRPYFHFLWHLCVLAGTSCHFVAIYRYGV"
     gene            7688..9337
                     /locus_tag="AC16YU_06830"
     CDS             7688..9337
                     /locus_tag="AC16YU_06830"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6064975.1"
                     /translation="MRLRTLIPALAALVPALLALATSATAQNDPFTRADTLRGSNGPG
                     RAWWDVTFYDLAVTVSPDDSTLEGSVAITYRVVGPAGEMQLDLQPPMQLHSVVLDGRE
                     RAVRRDGNAWFVDMPAQRPGDMHTVTARFGGAPRVAVRPPWDGGYQWERGEDGEVWVA
                     TSNQGLGASIWWPNKDFQADEPDSQRTAITVPDPLVAVSNGRLESSRPNGDGTTTWTW
                     FVSSPINNYSVEVNAGRYAHWTETYEGESGTLTMDYWPLEENLDRARAQWAQARPMMA
                     CFEHWFGPYPFYEDGYKLIEVPYLGMEHQSGVTYGNGFTNGYRGRDLSETGQGLKWDF
                     IIIHESAHEWWGNNITASDIADNWVHESFANYSENLYTECLTGSAEAGEEYVTGTRKL
                     VGNRRPIVGPYGVNRGGADIYYKGGNLLHMIRQLVRNDQAWRRILRGLNAEFRHRIVP
                     GTEVESYISRESGLDLDLVWDQYLRTPMIPVLEWHLEGGALAYRWQHVVHGFTMPVRV
                     SVGGGPEFWLRPTERWQVVEVGASGDLAVDGGFYVEVRRVE"
     gene            complement(9429..>9770)
                     /locus_tag="AC16YU_06835"
     CDS             complement(9429..>9770)
                     /locus_tag="AC16YU_06835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6064976.1"
                     /translation="NATIAPQEMGRVIVNMVTNAYDAMETVDAPRLTVSTRRSGGHVE
                     IRVTDNGPGIPDDIRAKIFEPFFTTKPTGKGTGLGLSLSHDVVTQGHGGTMTVEDAPD
                     GGASFVVRIPA"
BASE COUNT         1619 a         3386 c         3185 g         1580 t
ORIGIN      
        1 tcgcgacgat ctcccggggc ccctggctga acaccggggt ggtttccacc gggacccgat
       61 atagcgtgcc ggtatcgcgg tagtacaggt atttcccgtc cgcggaccag ctcggatccc
      121 aggcatcccc gaattccgga gccacctggt acagtgccgt tccgtccagg tcgcgaacgt
      181 cgaggccctg ggattcgaac gccacgtagc gtccgtccgg tgacaccatc ggatcgaagg
      241 ccgtcgtctc gctgaacaca tgctgaaggg tggtgtcggc caggtccacg attcgcccca
      301 cctcgtcatc ggtcgtgtag tacagcagcc gcctgccgtc gggggtgatc tccgggtccg
      361 cgagcgttcc cttgccgacg aggaccactt cttccgccgc cgacagatcg gctcgacgtc
      421 gaagtatgac gtcctttccg gcacgccgtc cgacatagta tatccaccgg ccgtcgggcg
      481 accacgtggg ccatccgctc ggtccatcga aggacaccct ctggctggat cgccttgcga
      541 ggtcgttcac gaatacccgg tattcgccgt ccgcgaacct ggcggccacg aaccggtcac
      601 cgtcacggga aatctccgcg tgctcgaccc cgctccgacc ccaatcaccg ctcgagcccc
      661 ctccatacgg caggatcatg ggacctacgg agaactcgtc ctgttcgccc accaggcgcc
      721 catcggttcc gatccagtag tacacgaact ccgatcgcga tgcggtggtg agcgcgtagg
      781 cgagggtccc gtcggcaccc actccgattt cgctgaaggg attcgaactg atccccgcgg
      841 gtacctcgac accggtgaac cgtgcccggg cagcatcgaa cgggcgcagc atgaacgtgc
      901 cgttggacat gggctgctgg gtatacaaca gatgtccgga cggcagatac cgcgcacagc
      961 agatgtcgtc cgccagtctc ctgagactgc ccgattcgag gtccactatc ccgagcgcga
     1021 ccccgttgaa ggccccgagc gtcgcgacca cctgccgttc gccggggagc ggatgggcat
     1081 accagatgac gtccacggga ggaatcgagt cggccgacac caccttgcgg gcgttctggc
     1141 cgtccacctc ggcgcgccac agacccagat cacttggggt gtacagaatc tcctcgtcat
     1201 tgagccaggt ggccgcggcg cccctcagcg gtatggtctg cggtcgatcg tcagaaagcc
     1261 gataccgaac cagtgggccg ttgatgtcga tgttcgatgc cagcagccat tcgccgctcg
     1321 gggagaactc aaggttgtcc gtaacctcca ggctgatcgg tcccgtccac tctccgatgc
     1381 cggtatcgta gaggacgatg ctccaatccc cctggccgcc ctgcgtcttc accgcgacca
     1441 ggccaccgtc cggagaaatg tccatgacac gggccgacgc cacgtcctcc acggccaggg
     1501 ggacggttcg cgtcggcggt ggcgcaggct cgggggatcg ggttgccagc cacgatcccg
     1561 ccgcaccgag gagcagcgcc ccgatgatcg ccccgtacgc ccacaacggg agctttcggc
     1621 ccggcgcgga cgccgccggc atccctgccg tgctcatcgc cggcatcgat cgcctggtca
     1681 ccccgcttcc ccccaggctc aggctcttga ggtcggcgag cagatccgcc gctgtctggt
     1741 accggtactc cgcctccttc gacaggagct tcgccacgat ccgctccagc tccatcggca
     1801 cccccgtccg caccgccgtc aggggctccg gaggttcgtt caggatcccg tagagcaccg
     1861 cctgctcgta gtcgcccccg aagggcagct tccccgccac catctcatag agcatcgtcc
     1921 cgagcgagta caggtccgtt cgcccatcca cctcgtggcc cctcgcctgc tccggcgaca
     1981 tgtacgccac ggtccccagc gtcgaaccca tccgcgtcag catcgtcgac tggttcgtct
     2041 tcgcgagacc gaagtcgagc accttcgcca cccccgcctg agtcagcatc acgttcgccg
     2101 acttgacgtc ccggtgcacg atctgcttcg cgtgggcctc tttcagcgcc tcggccacct
     2161 ggcccgccac gttcaccgcg tcatggagct tcatcgggcc gcgcttcacc atctcggcga
     2221 gcgtctcccc ctcgatgtac tccatcgcga tgaagaggcg ggactcggtc cccggctggg
     2281 gggcgacccc ctcctcgacc ggcacggcct cgtcgatccc gaagacggtc gcgatgttgg
     2341 ggtggtggag ctgggcggcg gactgggcct cgcgcacgaa ccgggcacgg tcgtcttcgc
     2401 tcgaaagggc ggccgcagga agcaccttga gcgcgaccgt gcggttgagc ttcgtgtcgg
     2461 tcgcacggta gacgatgccc atgccgccgc gtcctaattc ggcgtcgata cggtactggg
     2521 cgaggtggag tcctgtcacg cctgcggtag gagggtagcc gcgcaaggta ctccctccat
     2581 ccatgccttc acaatgtctt gacaatggaa ccgcccgaca ccgcctccta ctcctgccgc
     2641 agggtctcca ccgggttcgt gagcgcggcc cgcaccgtct gagtccccac cgtgaccagg
     2701 gcgagggcca cgatcgggag gattcccatc gcaaacagcc aggcgtcgaa ctcgacgtga
     2761 ttggggaatt tctccagcag cgcgcgaccc gccaggtagg agagtgggcc ggcgaccagg
     2821 acggcgaccg cgatcatccg gacatagtcg cgggacagca gcgcgaccgt gccccgaaca
     2881 tccgctccca gcaccttgcg gatacccact tcccggaacc gccgctgggc ggagtggctg
     2941 gccgcgccga ggagacccag gcaggcgatg aggatcacga aagcggccag gagacccagg
     3001 aatcgcacca agtcccgtac gaaggtgtac tgggacgcga tttcctcgtc gtagaagcgg
     3061 tacacgaaag gcgccgtcgg ggcgactttt tcgaacgcat cctcgagcgc accgaccgtc
     3121 gtctgcacgt ctccaggggc caggcgcacg acggcgcgct ggtaaccctc cggattcacc
     3181 cgcagcatgg ccggcgtgta cccccgctcg tagccccggg cgtagaaatc ggcgagcact
     3241 ccgaccaccg tccccggatg ggcctcccag gtgggactca cggtgagcgc cgcgcccacg
     3301 gcgtcccccg gctgctcgta tccgagcgcg cgcgtagcgg ctaccgtgag ccagaccggt
     3361 tccggatcca atcccggaac cacgccgtcg agcccgcgcc cctcgactac ctccagatga
     3421 agctgacgag ggaactctgc gtccacgctg aagaactgca gcagcaggtc gtcctctccg
     3481 ttttcgcccc gaatggacgt ccacaccttc gactccccgg acggcatcgt agacgtcgcg
     3541 gccacgccct ccaccccggc caggctcgaa aactcgtggg cgaccgcccc gaacggtgca
     3601 tcctgcaggt ccaccgagag cagacggtcc tccccgaacc cgtagtcggc ctggagcacc
     3661 atggacatct gccgatgcac cagcagggcc gtcacgactc ccacgattgc gacggcgaac
     3721 tggaagacga tcaggccacg gcggccaagc ccctcccggc gtccggcggc gcccgcaagc
     3781 cccctgagca cgggcgacgg cgcgatcgac gacatccgga acgccgggac gagcccggcg
     3841 accatgccga ccgcgacggc aaacccgatg tacagcaggt agatcagccc gtcccggaac
     3901 ggatcgaccg tgatgctgcc caggtccagg gcatcccgga aaccgctcag gctgttgaac
     3961 gccggcagga gccataccag catggccgcg gccacgacca gcgcgagcaa cgccacgacc
     4021 accgcctcga gagcgaactg cgccacgatc tgggcgcggg ctgcacccat gaccttgcgg
     4081 atgccgatct cccgcgctcg gcggatcgcc cgggcgatag tgagattcac gtagttgaat
     4141 accgcaatta tcagcaggat cgccccgaag aagagcagga agtacgaggc gtccttcggc
     4201 attaccggcc cgagctggtt cgagagatcc tcgcccaggt tgatgtcggt cagcgcctgc
     4261 atgccgatgg tcggacgggg gcgctctgaa ttccactcat gggcgtccac cagcgactgg
     4321 gcccgggcca ctatgtcctc cgtccgggcg ccgggggcga ggagcaggta cgtgtagagg
     4381 gtcgatgaaa cggtccagtc ttcgatgctg tacgggtcat ccgtgccgga cagcgccacg
     4441 agcgtcgaaa aggacgccag cgcctcgaag gcaaggtgcg tgttcgtggg tggatcctcg
     4501 agcacgcccg taaccgtgaa tccgccgagg tcgccacgct gcaggacctt cccgaccgga
     4561 tcggccccac cgaaataccg ctccgccgac gaccgggtca ggacgacgga atacggcgcg
     4621 gacagcgccg tcgccggatt tccccggacc atcgggaagt cgaagagttc gaagaacgag
     4681 ggctccgcgt agagtcctcc gagcggcagc gtcacgtctc cgtaggacgc gctgcctccc
     4741 atacgccgga gccgcgtcgc ccgttcgacg gacggcacgt cctcgagcaa cgccgggccc
     4801 agcgggcctg ccgaggtggc catgggaaaa cgaccgaacg gctcctccac ctccgaggtg
     4861 atccgccaga tccggtccgc attcgcgtgg aaccggtcat acgaaagctg ctcgcgaagg
     4921 agcgtgatca ccagcagtcc gaccgacatg ctcacggcca gcccgatgat gttgatggcc
     4981 gagtagccga ggctcttgcg caggttgcgc agtgcgacca ggaggttgac tctgagcatg
     5041 atgttggagc gccgggcgtg gcggaggcgg agatacagga ccaggtacag caggcgcgat
     5101 gcgtacacca ggcccgccgg ggcgcgaccg aatcgcctga cgtcgtcgag atgatcttct
     5161 ctcagatcgc cgtacaggct ctcgtactct ggcaggagcc atcgctcggc gagtctcgcg
     5221 atgaaccggg gcgggcgcgg ggacatgggc gcgggctgca tgacgggtca ggtctccgac
     5281 aggccggccc gcagccgggg aagacccgac cacgcgcgct cctcgagcag gcggacggct
     5341 tcgagtgcac ggacgccctc gcccgtcagg cggtagaacc gcttgcgccg gccgccgcgc
     5401 tcttccgtgg gctcgccctc ccacgattcc agaaggccgt ggccggcaag ccgctcgagc
     5461 ggaacgtaga ccgccccgac cgatacccgg cgcctgagca gctgcgacag cgctcgccgg
     5521 acagcgagcc cgtaggcctc gtcgccgagc ttccatacgg tgagcagcag cagttcctcg
     5581 atgcgtgtca ggtgatccac ggtcggcggc gcggtcgttc aggtatttgt tctactggat
     5641 aaaacttatc cgggttacag cccggaaggg cattatccgg taccccggaa gtgcgatcgt
     5701 cgggtcatgc gatcaacggc cccctggtca tgccgacgat ccgtcagtcc ggcttgatct
     5761 gttcggcgtt gctctccagc cagtcggcga agctctgcag ctccgggttc agggtccgcg
     5821 aaagatccac gtcacggaga gccaggaagt cctcgttgaa gtcgtggtgg tgctggaaca
     5881 tgttgccgag gtcctccgca cccggaaagc cgaacgagcg atacgtgtcg aaggggaccg
     5941 cgtagtagtc cacgggttcg tcgagagctg ctgccatggc ctcggccatc tgtgcccccg
     6001 tcaaccggcc gccggcaacc ccgaccctgc gaccgataaa gtcctctccc gccttgaaga
     6061 ctccgagcgc gcactttccg atgtcttcag ccgcaatgcc gggaagccgg gcgtcgccca
     6121 tgggaagcgc gaacacgagc cggccgtcct caccgcgccg gggtcccatg ccgaagtgga
     6181 tgagattgtc ccagtagaac gatgcggaga agaacgtcgt cgggacgccc tcctcggtga
     6241 agaagcgatc ggcctgccct ttcgcgtcga agtgcggaac cttgtacttc ccctggaggg
     6301 tcggcatacg gtcgtcgtcg agcggcaccc acttccgggt gtcctcaagc gtggaccaca
     6361 cggcgtgtct gatcccggcg cgttttgctg caatcgcaag gttctgcgcc tgctgtgtgt
     6421 ccttctcggg cgagaggtgc tcccagaaat tcgtcacgca gaacgctccg tggcatccgt
     6481 cgaatgcggc agcgaggctg tccacgtcgt ccaggtccgc ccgaacgacc tgcgcgccgc
     6541 gtgccgcgag ggcgcgggcc ttttcggagt ccggattccg ggtaatcgcc cgcagtgcga
     6601 actccccgcg ctcatcgtcc aggatcgccc tggccaggcc gccgccctgc gctcccgtcg
     6661 ccccgagaac cgctatcagt ttcttttcca tggtgcctgt caaaacaata gttgtttatc
     6721 cgcctttgta atggtacggg tgtcgccttg ttcctgcaag tcacccgggt ggtgatggcc
     6781 agggacactc gcgaagtcgg gacctcctgg tccgcgcgaa caacaggccg cgacaggcat
     6841 accgatcccc ttcagaccgc agcggatggc atggcccacc gatcacagag tcgcggcgag
     6901 gaatgggcga acgcgctcag ccacggagcc ggtgcgctcg gcgcggccgt cgcgctgccc
     6961 gtcctcgtta tcgaggcgct tcgttcaggc agcgcaatgg cggtggtcgg cgccgtggca
     7021 ttcgccgcgt cggctctctt cctgtacgcc gcgtccaccg cctaccatgc ggcgccgacc
     7081 gggcgcgcca agcgggtgct gcgccatctg gaccacgtgg gcatcttcgt cctgatcgcc
     7141 ggcagctaca ccccgttcat gctgggcccg ctccgggggc cgtggggctg gtcgctgctc
     7201 accgccgtct ggtcgctggc gctgctgggc atcgtgtgga aggtgcatcc acgactgcga
     7261 cgggaacgtg cgccggtttg gctctacctg ctgatggggt ggctcgtggt cgtcgccgtg
     7321 agaccgctct ggatccacgt gtccggtggc gcacttgcgt ggatggcgct cggtgggctg
     7381 gcctacaccg gcggcgtcct gttctacgcg accgaccgcc ggccctactt ccattttctg
     7441 tggcacctgt gcgtgttggc cggcacgtcg tgccacttcg tggccatcta ccggtacggg
     7501 gtgtaaggac gtcggctccg gggatcggtc ggccggtcgc gggtcatgcc cctgggtctc
     7561 cgggtggggc ccgatgcgct cttgtcaatc gggaacggcg acggcggatt cgtgtccgcg
     7621 gagtcgcgat attgccgccg cagcccgcac ctcgcaccgg caacccgcac ccgcctccct
     7681 ggacgccatg cgcctccgca cgctgatccc ggccctcgcc gcgctggtcc ccgccctgct
     7741 cgctctcgcc acttcggcca ccgcgcagaa cgacccgttc acgagggccg acaccttgcg
     7801 cggctcgaac ggccccggca gggcctggtg ggacgtgacg ttctacgacc tcgccgtcac
     7861 cgtatcaccg gacgacagca cgctcgaggg atcggtcgcg atcacgtacc gagtcgtggg
     7921 ccccgcgggc gagatgcagc tggacctgca gccgcccatg cagctgcatt ccgtggtgct
     7981 cgacggccgg gagcgggccg tgcggaggga cggcaacgcg tggttcgtcg acatgcccgc
     8041 gcagcggccg ggcgacatgc acacggtcac tgcgcgcttc ggcggcgcgc ctcgcgtcgc
     8101 ggtccgaccc ccgtgggacg gcgggtacca gtgggagcgc ggcgaggacg gcgaggtctg
     8161 ggtcgccacg tccaaccagg gcctgggcgc cagcatatgg tggcccaaca aggacttcca
     8221 ggccgacgag ccggacagcc agcggaccgc catcaccgtg cccgatccgc tggtcgccgt
     8281 ctcgaacggg cgcctcgaga gcagccgccc gaacggcgac ggcacgacca cctggacgtg
     8341 gttcgtctcg agcccgatca acaactactc cgtcgaggtg aacgccggcc gatacgcgca
     8401 ctggacggag acgtacgagg gcgagtcggg caccctgacc atggactact ggccgctcga
     8461 ggagaacctg gacagggcgc gcgcgcagtg ggcccaggcc cgcccgatga tggcctgctt
     8521 cgagcactgg ttcggaccgt accccttcta cgaggacggg tacaagctca tcgaggtgcc
     8581 ctacctcggg atggagcacc agagcggcgt gacctacggc aacggattca ccaacgggta
     8641 ccgcggccgg gacctttccg agaccggcca ggggctcaag tgggacttca tcatcatcca
     8701 cgagagcgcc cacgagtggt ggggcaacaa catcaccgcg agcgacatcg ccgacaactg
     8761 ggtgcacgag agcttcgcca actactcgga gaacctgtac accgagtgcc tgacgggctc
     8821 cgccgaggcc ggcgaggagt acgtgaccgg cacgcggaag ctcgtcggga accgtcggcc
     8881 catcgtgggg ccgtacgggg tgaatcgggg cggcgccgac atctattaca agggcggtaa
     8941 cctgctccac atgatccgcc agctcgtccg caacgaccag gcgtggcggc gcatcctgcg
     9001 cgggctcaac gccgagttcc ggcaccggat cgtgccgggc acggaagtcg agtcttacat
     9061 ctcgcgcgag tccggactgg acctggacct ggtctgggac cagtacctgc gcacccccat
     9121 gattcccgtg ctggagtggc acctggaggg cggagccctc gcctaccggt ggcagcacgt
     9181 tgtccacggg ttcacgatgc cggtccgggt gtcggtcggc ggcggccccg agttctggct
     9241 ccggccgacg gagcggtggc aggtggtgga ggtgggggct tcgggtgatc tcgcggtgga
     9301 cgggggattt tacgtggagg tgcggcgggt ggaatagcgg ggcaggggca gccgccggac
     9361 gtggccacgt tacacgtcca cctcggcggc gctccagcca tggtccaggt ctaaccgccc
     9421 tgccgcgatc aggccggaat ccggaccacg aacgacgcgc caccgtcggg cgcgtcctcc
     9481 acggtcatgg tacctccgtg cccctgggtg acgacgtcgt gcgagaggct caggccgagg
     9541 cccgttccct tgcccgtcgg cttcgtggtg aagaacggct cgaagatctt ggcgcggatg
     9601 tcgtcgggaa tgccggggcc gttgtcggtg acacggattt caacgtggcc gccgctgcgc
     9661 ctggtcgaca cggtcagcct gggcgcatcg acggtctcca tggcgtcgta cgcgttcgtc
     9721 accatgttca cgatcacacg acccatctcc tgcggcgcga tggtggcgtt
//
LOCUS       DBNHQZ010000254         3345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2242342, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000254 DBNHQZ010000000
VERSION     DBNHQZ010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2242342"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2284)
                     /gene="ileS"
                     /locus_tag="AC16YU_06840"
     CDS             complement(<1..2284)
                     /gene="ileS"
                     /locus_tag="AC16YU_06840"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR6064977.1"
                     /translation="MPRFKEIKRLNLPELEEGVLDWWQKENIFERSVEQHRGEPTFSF
                     YDGPPTANGRPGIHHVLARTIKDSFCRYKTMKGFQVARKAGWDTHGLPVEIEVEKALG
                     LDGRHQVEEYGIERYNRACRESVLRYTGVWDELTLRMGYWVDLRNPYVTFRPEYIESV
                     WWLLKQIWEKGLLYKGYKIQWYSPGSGTVLSSHEVSLGYREIQDPSIYTRFRSVEDDD
                     LYYLAWTTTPWTTISNAALAVGNGIEYVRIRLATEDIGQHDLVLAKERLEVIREEYEI
                     VDTMWGRDLVGRRYRPLFPYFEGRPETRDAWRIVPAVYVSTADGTGIVHTAPAFGAED
                     FDTGRAEGLPMLNPVGPDGRFSDEIGLVAGMWFKDADKPIARDLRERGLLYRHETYVH
                     NYPHDWRKDTPLISYPVEGWFIRTTAIKDRLVELNRTINWQPAGIGTGRFGDWLENNV
                     DWALSRRRYWGTPLPIWQSDAPGSEFVEVIGSIAELREKCGDQLPADDAGIDLHRPFV
                     DGLTWPAPDGGTMRRVEDLIDVWFDSGAMPFAQWHYPFENRDAFEATFPADFIAEGVD
                     QTRGWFYTLHAIAALVMDGVAYRNVVVNGLVLDENGEKMSKRLGNTVDPFTTIREHGA
                     DVVRWYMMSNAPPWENLKFSERGLQESSRKFFGTLANVYAFFATYANVDGFDGTLPET
                     PVEGRPEMDRWILSRLASTVAEVDEALDAYNPTRAARSVEQFVDALSNWYIRRNRRRF
                     WSGKQADRGPDATHKTAAYQT"
     gene            complement(2397..3110)
                     /locus_tag="AC16YU_06845"
     CDS             complement(2397..3110)
                     /locus_tag="AC16YU_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase family protein"
                     /protein_id="HIR6064978.1"
                     /translation="MPDPPEDSPDDSLLDRAVQSATHAGLSGRRSAIVLEGRRFPAPG
                     DREGLEVAGGLLSARAFGADSDPLLLAAGPGLVRDPLLAVRSLADLGVRHVLFLADAD
                     PVSAAVADPGMALVRDHVNLRAANPLVGPNDPRFGPRFPDLSEAYAPALRQRLSRTAG
                     LEEEGIVVGAVDREEARSVLAHSDFWRGLGIHALTAGIVPGAIVARHAGLRVAAVVAF
                     RAVHPDELLALARAFAAHD"
     gene            complement(3116..>3345)
                     /locus_tag="AC16YU_06850"
     CDS             complement(3116..>3345)
                     /locus_tag="AC16YU_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064979.1"
                     /translation="PEEEEESFDIRDMADLEAEPVEVPDGGFDEDSGDDATHDDGPEP
                     EASSAEEPEDEAFGAEDEIRKIRRILEDLDG"
BASE COUNT          518 a         1163 c         1115 g          549 t
ORIGIN      
        1 ccgtctggta ggccgccgtc ttgtgggtcg catccgggcc ccggtctgcc tgcttcccgg
       61 accagaagcg ccgccggttg cgccggatgt accagttcga cagggcatcc acgaactgct
      121 cgacggaccg cgccgcgcgc gtcgggttgt aggcgtcgag cgcctcgtcc acttccgcga
      181 ccgtcgacgc gagccgggac aggatccacc ggtccatctc cggccggcct tcgacgggcg
      241 tctcggggag cgtgccgtcg aacccgtcca cgttcgcgta ggtcgcgaag aaggcataca
      301 cgttcgccag ggtgccgaag aacttccggc tcgactcctg gaggccccgc tcggagaact
      361 tcaggttctc ccacggcggc gcgttcgaca tcatgtacca ccgcacgacg tcggcaccgt
      421 gctcgcggat cgtcgtgaac gggtccaccg tgttgccgag ccgcttggac atcttctcgc
      481 cgttctcgtc gagaacgagg ccgttcacca cgacgttgcg gtaggcgacg ccgtccatga
      541 cgagcgccgc gatggcgtgc agggtgtaga accagccgcg cgtctggtcc accccctcgg
      601 cgatgaagtc ggcggggaac gtggcctcga aggcgtcccg gttttcgaac ggatagtgcc
      661 actgggcgaa aggcatcgcc cccgagtcga accacacgtc gatcaggtcc tccacgcggc
      721 gcatcgtgcc cccgtcgggg gccggccacg tgaggccgtc cacgaacgga cgatggaggt
      781 cgatcccggc gtcgtcggcc gggagctggt cgccgcactt ctcgcggagc tccgcgatgg
      841 acccgatcac ctccacgaac tccgaccccg gggcatccga ctgccagatc gggagcggcg
      901 tgccccagta tctgcggcgg gagagcgccc agtccacgtt gttctccagc cagtcgccga
      961 agcgccccgt cccgatcccg gccggctgcc agttgatggt acggttcagc tcgacgagcc
     1021 ggtccttgat ggcggtcgtc cggatgaacc agccttcgac cgggtagctg atgagcggcg
     1081 tgtccttgcg ccagtcatgc ggatagttgt ggacgtacgt ctcgtgcctg tacagcagac
     1141 cgcgctcgcg caggtcgcgg gcgatgggct tgtccgcgtc cttgaaccac atcccggcga
     1201 cgaggccgat ctcgtccgag aagcggccgt ccgggccgac cgggttgagc atcgggaggc
     1261 cctccgcccg cccggtgtcg aagtcctcgg ctccgaatgc cggagccgtg tgcacgatgc
     1321 ccgttccgtc ggccgtcgag acgtacacgg ccgggacgat gcgccacgcg tcgcgcgtct
     1381 cgggccttcc ctcgaagtac gggaacagcg gccggtatcg ccttccgacg agatcgcggc
     1441 cccacatcgt gtcgacgatc tcgtactcct cccggatcac ttccaggcgc tccttggcca
     1501 ggacgaggtc gtgttgcccg atgtcctcgg tggcgagccg gatgcggacg tactcgatcc
     1561 cgttgccgac ggcgagcgcc gcgttcgata tcgtggtcca gggcgtggtg gtccacgcca
     1621 ggtagtagag gtcgtcgtcc tccacggaac ggaaccgggt gtagatgctc ggatcctgga
     1681 tttcccggta gccgagactc acttcgtgcg aggacagcac cgtccccgac ccggggctgt
     1741 accactggat cttgtacccc ttgtagagca ggcccttctc ccagatctgc ttcaacagcc
     1801 accagacgct ctcgatgtac tcggggcgga acgtcacgta gggattccgg aggtccaccc
     1861 agtatcccat ccggagcgtg agttcgtccc agacgccggt gtaccggagc acgctctccc
     1921 ggcaggcgcg gttgtaccgc tcgatgccgt attcctcgac ctggtgccgt ccgtcgagac
     1981 cgagcgcctt ctcgacctcg atctcgaccg gaagcccgtg ggtatcccag cctgccttgc
     2041 gggccacctg gaagcccttc atggtcttgt accggcagaa gctgtccttg atggtccggg
     2101 caagcacgtg gtgaatgccc ggacggccgt ttgccgtcgg aggcccatcg tagaacgaga
     2161 acgtgggctc gccccggtgc tgctcgacgc tgcgctcgaa gatgttctcc ttctgccacc
     2221 agtccagaac gccctcctcg agctcgggca ggttgagtcg cttgatttcc ttgaagcgtg
     2281 gcatggggtg gatacggcct tcgacgacgg tcgacgtggg agtgcaatga aagcctgcaa
     2341 aatacggaaa cccgggagag ggacgagcca cgcggacgcc ccggcggccg gattcgtcaa
     2401 tcgtgggcgg cgaaggcccg ggccagcgca agcagctcgt ccgggtgcac ggcgcggaac
     2461 gcgacgaccg ccgcaaccct gagtccggca tgtcttgcca cgatcgctcc gggcacgatc
     2521 ccggccgtca gcgcgtgaat gccgagtccg cgccagaagt cggagtgcgc gagaaccgag
     2581 cgcgcctcct cccggtccac agcgcccacc acgattcctt cctcctccag gccggcggtc
     2641 cgcgaaaggc gctgccgcag cgcgggagcg tacgcctcgc tcaggtcggg aaaccggggg
     2701 ccgaagcgcg ggtcgttcgg gcccacgagc ggattggcgg cgcgcaggtt cacgtggtca
     2761 cggacgaggg ccatccccgg gtccgcgacg gcggcgctga cgggatcggc atccgcgaga
     2821 aagagcacat gccggacgcc caggtccgcg agcgagcgga cggcgagcag cggatcccgg
     2881 acgagtccgg gcccggcagc gagcagcagg gggtccgagt ccgccccgaa cgccctcgcc
     2941 gagagcaggc caccggctac ctccagcccc tcccggtcac cgggcgccgg aaagcgccga
     3001 ccctcgagca caatggcgga tcgtcgtccg gagaggccgg cgtgggtcgc cgactggacc
     3061 gcccggtcca gcagggagtc gtccggcgaa tcctccggcg gatcaggcat cgacctcacc
     3121 cgtcgaggtc ttcgagaatg cgccggatct tgcggatttc gtcctcggcc ccgaaggcct
     3181 cgtcctcggg ctcttcagcc gacgacgctt cgggctcggg cccgtcatcg tgggtggcgt
     3241 catccccgga atcctcgtcg aagcccccat cgggcacttc gacgggctcc gcctcgagat
     3301 cggccatgtc ccggatatcg aacgattcct cctcctcctc gggct
//
LOCUS       DBNHQZ010000255         3392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_347605, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000255 DBNHQZ010000000
VERSION     DBNHQZ010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_347605"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1272
                     /locus_tag="AC16YU_06855"
     CDS             202..1272
                     /locus_tag="AC16YU_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR6064980.1"
                     /translation="MASAPAEVTLEILPRNRVDVIDVADLVNRQTGGFFDEYEKSVFC
                     SYHTTAGYFEQSLISRLGHNADAVQAFVQSFSELFPAGAGYRHDEMHLRAELTDEQKQ
                     VEPRNADSHLTFIGCGLENCVTYLNRPQAPVYFVELDGVNGPDRRRRRTSVIGFTRAE
                     TIDRFALEIPVSGHRIDSINLWDPALGLYDRLHELVRIHGVSKGRIDIALDPRERNAG
                     LTVNEYETLLMRHDLAEVLRNPIRFMAQKGRNMLRDPRAIPDKARDYAKYDLVRVVNE
                     FIDAMGLNESLIERIIDRFLAVPAARFLRMKRGVSLVVNGAETGTPGRIISGTYQSPI
                     LVQWRKADRQVRHVEVTYTRFV"
     gene            complement(1277..2053)
                     /locus_tag="AC16YU_06860"
     CDS             complement(1277..2053)
                     /locus_tag="AC16YU_06860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6064981.1"
                     /translation="MDLQLAGKSALVTGASRGIGLGIATSLALEGCRVAICARGEEAL
                     ESAAGELSERGADVVALAADVTDAAQAEAVVDHAAEAFGGLDILVGNVGGNRRGRFEE
                     ATDDDWEAVLDLNLRAHVRVARACIPVMKAGGGGAITFIASIFGREAGGPGLALYNST
                     KSALISLAKIMALELAGDGIRVNSVAPGSIRFPGGSWDRRCIEDPDGMARFIEANLPV
                     GRFGRVDEVADVVTFLSSPRASWINGACLNVDGLQSRSLI"
     gene            2145..2369
                     /gene="xseB"
                     /locus_tag="AC16YU_06865"
     CDS             2145..2369
                     /gene="xseB"
                     /locus_tag="AC16YU_06865"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6064982.1"
                     /translation="MSEVEPGSDMTAASFEEAIGRLEAIAQRLEHPDTPLEEAVALYE
                     EGVRLSRLCADRLEAAELKVTELRPGEPGE"
     gene            2455..3366
                     /locus_tag="AC16YU_06870"
     CDS             2455..3366
                     /locus_tag="AC16YU_06870"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6064983.1"
                     /translation="MKPSTIASLLAALALLPAMRASGQMYINQKELAEILRPCAAPGE
                     ESAHVDYKYAVLYSSRNNSVLARNTFYKILGDGDQELGTLRAKLVGLLNRTLIHQAEL
                     GDTLVVPARFEDDFCAYSPFPRVYEGASRYGKLFIIDKSVQAWAAYEKGRLVRWGIVN
                     TGSKESPTPNGRFNFNWKTEYRVSSLSPPGEQWEMYWVFNFHLARGIHIHQYPMPTGG
                     PTSHGCVRLVEEDAEWIYNWADAWTTTAGYGSLATEARVIRPGTTVLVIGEDPPVEPE
                     PFLRADGRPQLRMIELPERPEDVPLGT"
BASE COUNT          569 a         1198 c         1116 g          509 t
ORIGIN      
        1 tcgcgctcgt gcggcgacgc ggccgccccg tcgcgaacgt cggcatgctg cttgccgggg
       61 attccgtcga ggtacgattc cgcgacggga ccgccgacat ggaggtcacc gggacccgtc
      121 ccggccctac cttgcccgct ccggacacgt gaacccatgt ctgccgtttc agcggcgtaa
      181 tccaccggaa agcccctcgc catggcgtcc gcccctgccg aagtaaccct cgagatactc
      241 ccgcggaacc gggtggacgt gatcgatgtc gcggacctcg tcaaccggca gacggggggc
      301 ttcttcgacg agtacgagaa gagcgttttc tgctcgtacc acaccacggc gggctatttc
      361 gagcagagcc tcatcagccg gctcgggcac aacgccgacg ccgtccaggc cttcgtgcag
      421 tcgttctcgg agctgtttcc ggcgggcgcc gggtaccggc acgacgagat gcacctgcgg
      481 gcggagctca cggacgagca gaagcaggtc gaaccgcgca acgccgactc gcacctgacc
      541 ttcatcggct gcgggctcga gaactgcgtc acgtacctga accgcccgca ggcacccgtc
      601 tacttcgtcg agctcgacgg cgtgaacggt cccgacagga ggcgccgccg gacatcggtg
      661 atcgggttca cgcgcgcgga gacgatcgac cgcttcgccc tcgagatccc cgtctccggg
      721 caccggatcg actcgatcaa cctctgggat ccggccctcg gcctgtacga caggctgcac
      781 gagctcgtcc ggatccacgg cgtctcgaag gggcgcatcg acatcgccct cgatccgcgc
      841 gagcggaacg cggggctgac ggtgaacgag tacgagacgc ttctcatgcg ccacgacctc
      901 gcggaggttc ttcgcaaccc gatccgcttc atggcgcaga agggccgcaa catgctgcgc
      961 gatcctcggg cgattccgga caaggcgcgc gactacgcga agtacgacct ggtgcgtgtc
     1021 gtcaacgagt tcatcgacgc gatggggctg aacgagtcgc tcatcgagcg gatcatcgac
     1081 cggttcctcg ccgtgcccgc cgcccgcttc cttcgcatga agcggggcgt gagcctggtc
     1141 gtgaacggcg ccgaaaccgg gacgcccggg cgcatcatca gcggcacgta ccagagcccc
     1201 atcctcgtgc agtggcggaa ggccgaccgg caggtccggc acgtcgaggt cacgtacacc
     1261 cggttcgtct gaccgctcag atcagggagc gggactggag gccgtcgacg ttcaggcagg
     1321 ccccgttgat ccacgaggcg cgcggcgacg aaaggaaggt cacgacgtcg gccacctcgt
     1381 ccacgcgtcc gaagcggccg accggaaggt tggcctcgat gaagcgggcc atgccgtcgg
     1441 gatcctcgat gcatctccgg tcccagcttc ccccggggaa gcggatggaa cccggggcga
     1501 ccgagttcac ccggatgccg tcgccggcga gctcgagggc catgatcttc gcgagcgaga
     1561 tcagcgccga cttcgtgctg ttgtacagcg cgaggcccgg accgcccgcc tcgcgtccga
     1621 agatcgaggc gatgaacgtg atggccccgc ccccgcccgc cttcatcacc gggatgcagg
     1681 cgcgggccac ccgcacgtgg gcacggagat tcagatccag gaccgcctcc cagtcgtcgt
     1741 ccgtcgcttc ctcgaaccgg cccctccggt tgcccccgac gttgccgacc aggatgtcca
     1801 ggcccccgaa ggcttcggcc gcgtggtcca cgacggcctc ggcctgcgcg gcatccgtga
     1861 catcggcggc gagggcgacg acgtcggcgc cccgctcgct gagctctccg gccgcggact
     1921 cgagcgcctc ctctccccgc gcgcagatcg ccacgcggca tccctccagg gccagggagg
     1981 tcgcgatgcc cagcccgatg ccgcggctcg ctccggttac gagggctgac tttccggcga
     2041 gttgaagatc catgagaggg gccgtcgatg gtcgattcgg gcagtatacg cgatctttcg
     2101 cgtgcgcatg gcgtaatgag agtcaatttg cacggagcgg aaccatgtcc gaagtcgagc
     2161 ccggcagcga tatgacggcg gcgagcttcg aggaggcgat cggccgcctc gaggcgatcg
     2221 cgcagcgcct cgagcatccg gacaccccgc ttgaagaggc cgtggccctg tacgaggagg
     2281 gggtccgcct gtcgaggctc tgcgccgatc gcctggaggc ggccgagctc aaggtcaccg
     2341 agctccgtcc cggcgagccg ggcgagtgac aggccgcccc ggcgcgcccc gggaccccgc
     2401 acgccgtttc gcgcgttcgt ctgttctccg aacacccaga gtcccctgag caccgtgaag
     2461 ccatcgacga tcgcaagtct cctcgcagcg ctcgccctgc tcccggcgat gcgtgcctcc
     2521 gggcagatgt acatcaacca gaaggagctc gcggaaatcc tgcggccgtg tgccgccccg
     2581 ggcgaggagt cggcccatgt cgactacaag tacgccgtct tgtacagctc gcgcaacaac
     2641 tccgtcctcg cgcggaacac cttctacaag atactggggg acggagacca ggaactcggc
     2701 accctccggg ccaagctcgt gggcctgctg aaccgcacgc tcatccacca ggcggaactc
     2761 ggcgacaccc tggtggtgcc cgcccggttc gaggacgact tctgcgccta ctcgcccttc
     2821 cccagggtct acgagggcgc gtcgcggtac ggaaagctct tcatcatcga caagtcggtc
     2881 caggcatggg cagcctacga aaaggggcgc ctggtgcgct ggggcatcgt gaacacgggc
     2941 tcgaaggagt cgcccactcc caacggccgc ttcaacttca actggaagac ggagtaccgc
     3001 gtctcctccc tctcgccccc cggcgagcag tgggagatgt actgggtgtt caatttccac
     3061 ctggcccggg gcatccacat ccaccagtac ccgatgccca ccgggggccc cacgagccac
     3121 ggctgcgtcc gcctggtcga ggaggacgcg gagtggatct acaactgggc cgacgcctgg
     3181 acgaccaccg cgggatacgg gtcgctcgcg acggaggccc gcgtgatccg gcccggcacg
     3241 accgtgctcg tgatcgggga ggatccgccg gtggaaccgg agcccttcct gagggcggac
     3301 ggccgtccgc aactccgcat gatcgagctt cccgagcgcc cggaggacgt ccccctcgga
     3361 acgtgaacgc ctagcggacg ccggagaaca gg
//
LOCUS       DBNHQZ010000256         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1837803, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000256 DBNHQZ010000000
VERSION     DBNHQZ010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1837803"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16YU_06875"
     CDS             complement(<1..230)
                     /locus_tag="AC16YU_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064984.1"
                     /translation="MKRVAFERYLQRVKMTLGRDTDVLGVLHVGPVLKAADDRVDVRE
                     IDLLIVVREDLVPRYLEDVSWLPGAEEILASAS"
     gene            520..1497
                     /locus_tag="AC16YU_06880"
     CDS             520..1497
                     /locus_tag="AC16YU_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064985.1"
                     /translation="MKNHTIRLYQLSAAILLSGVLVAGSAGDALAQNRAALLTEVSGS
                     VQVIRASGGGAVAASWGMQLFEGDEVTTGADGSAAVLYADNRLASVGTNGSLTVSASG
                     GGAGASRSVSSDRIADVSDLTLHRAGEGEIAALGGLRAGARREAIVAINPRHANVLAE
                     RPEFRWEAASEFEEFRISVRDATGEIWAADGVSSGIVYPSDAPALEPGRQYYWQVTGI
                     MMLDEVDSGLSPFVVLDEDERRAVNESVAEITSVLAENGDENSRDFLLGSLYSREGLL
                     GEAVEAFGRIAERHPDAALAHEILGKLYFDMGLKDQSIESLTKALASSR"
     gene            1494..>2193
                     /locus_tag="AC16YU_06885"
     CDS             1494..>2193
                     /locus_tag="AC16YU_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064986.1"
                     /translation="MRLGPNHILWVFLVAVPFAAPPSAFARQADDSLAVLLRENALGG
                     DTAAVDGLVAEHRLAIRPAVDSWIGEGLRAELVGDRESAGRILGAARLVADRFREIHG
                     ERSLSIGVGYVAGWNPGEMATKLLADSLSDAALAARTSADTREAALADYERALDLYRS
                     IGDGRGVANVLGGMGYVYFFLDRPKYLELNLQAVRAREAVDDQQLLGNAHSDVGTAYW
                     RFEGDLERARSHYET"
BASE COUNT          325 a          740 c          772 g          356 t
ORIGIN      
        1 gaggccgacg ccaggatttc ctcggcgccc ggaagccatg aaacgtcctc caggtagcgt
       61 ggaaccaggt cttcgcgcac gacgatgaga aggtcgattt cgcgaacgtc gacccgatcg
      121 tcggcagcct tcaggacggg tccgacgtgc aggacgccga gcacgtccgt gtcgcggccg
      181 agcgtcatct tgacgcgctg cagatatcgt tcgaaggcta cgcgcttcat ggctctcctg
      241 gtcgggcggc ggggacgtcc gtgacggatg agcatacgga cggggccgcg gacaggctac
      301 cggggaatcc acggccggcg gaccgttacg ctttcgtgac gcagttgtga atctgctccg
      361 ccgttcgttt cattgcgatg ctgtcgctac cccaggaatc gcgccgtcgc ttcgggatcc
      421 gccccgcgga ccccggctct gcaacattcc cttcggggct cccgtgtacc gtgggcatac
      481 ccccgcccgc aggttttctc cgtcaccagg ccgatcaaca tgaagaacca taccatccga
      541 ctgtaccagc tgtccgcagc gatcctgctc tcgggcgtcc tcgtcgccgg ctcggcgggc
      601 gacgcgctcg cacagaatcg ggccgcgctc ctgaccgagg tctccggctc cgtgcaggtg
      661 atcagggcct cgggaggcgg cgcggtcgcc gcttcgtggg gcatgcagct tttcgagggc
      721 gacgaggtga cgaccggcgc cgacgggtcg gccgccgtgc tgtacgcgga caaccgcctc
      781 gcctcggtcg gcacgaacgg ctcgctcacg gtctcggctt caggaggagg ggccggcgcg
      841 tcccgctcgg tatcgtcgga ccggatcgcc gacgtatccg atctcacgct ccaccgggcc
      901 ggcgagggcg agatcgcggc gctcggcggc ctgcgggccg gagcacgccg cgaggcgatc
      961 gtcgcgatca acccgcgcca cgccaacgtg ctcgccgagc gccccgagtt ccgctgggag
     1021 gccgcctccg agttcgagga gttccggatc agcgttcgcg acgcgacggg cgagatctgg
     1081 gccgcggacg gcgtgtcatc cggcatcgtg tacccctcgg acgcaccggc gctggagccg
     1141 ggccgccagt attactggca ggtcacaggc atcatgatgc tcgacgaggt cgactcgggg
     1201 ctctctccct tcgtggtgct cgacgaggac gagcggcgcg cggtgaacga gagcgtcgcc
     1261 gaaatcacct ccgtcctcgc tgagaacggg gacgagaaca gccgcgactt cctcctcggc
     1321 tcgctgtatt cgcgcgaagg gcttctcggc gaggccgtcg aggccttcgg ccgcatcgcg
     1381 gagcgccatc ccgacgccgc gctggcccac gagatcctgg gcaagctcta cttcgacatg
     1441 gggctgaagg accagtccat cgagtctctc acgaaggcgc tcgcgtccag ccgttgagac
     1501 tgggtccgaa ccatatcctg tgggtattcc tggtggcggt gccgttcgcg gcgccgccat
     1561 cggcctttgc gaggcaggcc gacgattcac tcgccgtgct cctgcgcgaa aatgcgttgg
     1621 gcggcgacac ggccgcggtc gacggcctgg tcgcggaaca ccggctggcg atccgcccgg
     1681 cggtcgattc gtggatcggc gaggggcttc gtgccgaatt ggtcggcgat cgggaatcgg
     1741 ccgggcggat cctcggggcg gcccgcctgg tggccgaccg ctttcgggag atccacggtg
     1801 agcgcagcct ctccatcggc gtgggttacg tcgccggctg gaacccgggc gagatggcga
     1861 ccaagctcct ggcggacagc ctgtccgatg cggccctggc ggcccggacg agcgcggaca
     1921 cccgcgaggc ggccctggcc gattacgaac gggcgctcga cctgtaccgg tcgatcggtg
     1981 acgggcgggg cgtggccaac gtcctcggag ggatgggcta cgtgtatttc ttcctggacc
     2041 ggccgaagta cctggagctg aacctgcagg ccgtccgggc ccgggaggcc gtggacgacc
     2101 agcagctgct cggaaacgcg cacagcgacg tgggaaccgc ctactggcgc ttcgagggtg
     2161 accttgagcg ggcccgctcc cactacgaga cgt
//
LOCUS       DBNHQZ010000257         4480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1974446, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000257 DBNHQZ010000000
VERSION     DBNHQZ010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1974446"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC16YU_06890"
     CDS             <1..415
                     /locus_tag="AC16YU_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6064987.1"
                     /translation="VTPNQVKAEAVADMRDALSGWTFVEEHHRAAAEAFVEGYARSTS
                     GEYGSLGHFVGMATHDVGTIDGPLRAGMVFTIEPALRVPEEQLYIRNEDLIIIHEDRA
                     EIVSIDLAMSIEEIEALMAEPGLLQTYPSAYRAGR"
     gene            476..1945
                     /locus_tag="AC16YU_06895"
     CDS             476..1945
                     /locus_tag="AC16YU_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR6064988.1"
                     /translation="MRILSLAALLSALLAAAPAATPAAAQDAPLFTRDFPPEEFAQRR
                     ERVYDAIGPDAVAIIQGEPSPRGYVRFRQSNEFYYLSGIEVPHAYLLLDGANRRAVVF
                     LPHRDARRESSEGRMLSADEPDLVRDLSGIDAVAAAEELSTALHGMLRRDRLPTVYTL
                     FKPAEGLAESRDLGVRRINDRLADAWDGRPSREAHFMGLLRERLPGVRIADLHPILDG
                     LRLIKSPREMEMIRRATRLSVAAIEEAMRSVEPGVVEREIDALAKFIYFRDGAQGDAY
                     YSLVASGPNAWYPHYHKGARTMQAGEMVLMDYAPDVGYYMSDITRMMPVDGRFTDAHR
                     ALYGFYERAYRAILDHIRPGATPNQIKAEAVADMREALSGWTFVEEHHRAAAEAFVEG
                     YAQSARGEYGSLGHFVGMATHDVGTIDGPLREGMVFTIEPALRVPEEQLYIRMEDLII
                     IHADRAEIVSIDLPMSVEDIENLMAEPGLLQTRPSTVMR"
     gene            complement(2011..3111)
                     /locus_tag="AC16YU_06900"
     CDS             complement(2011..3111)
                     /locus_tag="AC16YU_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6064989.1"
                     /translation="MPLLLLASGLVLLVAGAELLVRGASRLAAVFGVPPLVIGLTIVA
                     FGTSAPEMAVSAVAAAGGRGDLAVGNVLGSNILNVLLILGVSAVIAPLNVARQLVRLD
                     VPVVVALSIGVWALASNGTVGAAEGVLLMAGLVAYTAFLVRKGRADASLGAALVAAMP
                     ADASAPTERPRWQVGLLNGVLIAVGLGLLVLGSDLMVDGATEIARRLGVNELTIGLTV
                     VALGTSLPELATSVLASVRGERDLAVGNVVGSNLFNLSGVLGVAAVAAPGGLAVSEEV
                     LRFDFPIVIAAAVACLPIFFTGHRIDRWEGALFLAYYVAYTAYVVAAAIGNSPIDELV
                     TVMLWFVVPLTVLTLGVLGVREMRRPDFGADA"
     gene            complement(3122..3607)
                     /locus_tag="AC16YU_06905"
     CDS             complement(3122..3607)
                     /locus_tag="AC16YU_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064990.1"
                     /translation="MLRTVLAVVAGYAAMFIGVMVLFTVYYLLIGVEATYKPGTYEVT
                     ALWIIGSIILSFVAAYAGGYVCTVLARNRLAAKVLAGIVLVLGVVMTFMWTSEGVGAV
                     RLGDVSMSDAMSMSVEPRWLTFLNPFLGALGVLLGAARVRAAAPAPAATIAARTATED
                     E"
     gene            3761..4135
                     /locus_tag="AC16YU_06910"
     CDS             3761..4135
                     /locus_tag="AC16YU_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064991.1"
                     /translation="MILRLLPAILSLVVLGAHFLRLGNLVVVALLIACIGLLWVRRPW
                     AAYSVQTILAMGIVVWVLTGYGIARVRIQNGEPWIRMAVILGAVAAMALYAIVSFRRP
                     RIRAWFGTDQPGAADQPTVEPS"
     gene            4175..>4480
                     /locus_tag="AC16YU_06915"
     CDS             4175..>4480
                     /locus_tag="AC16YU_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064992.1"
                     /translation="MPEPPSDRIYTDDEIARILKRAADLQGRESRRPVLGLTLPELQQ
                     LARESDIDPALITAAALELAGIPDQEEAGFWGGPLTVTIRRRVEGSVSEDAFNRMVSE
                     "
BASE COUNT          747 a         1616 c         1501 g          616 t
ORIGIN      
        1 cgtgactccg aaccaggtca aggccgaggc cgtggccgac atgcgcgacg ccctgtccgg
       61 ctggaccttc gtcgaggagc accaccgggc ggccgccgag gctttcgtcg agggatatgc
      121 ccgatccacg agcggcgagt acggcagcct ggggcatttc gtgggcatgg cgacgcacga
      181 cgtcggcacg atcgacggcc cactgcgcgc cggcatggtg ttcacgatcg agcccgcgct
      241 ccgcgtgccc gaggaacagc tctacatccg gaacgaggat ctcatcatca tccacgagga
      301 ccgcgcggag atcgtctcga tcgacctggc catgtcgatc gaggagatcg aggcgctcat
      361 ggccgagccg ggcctcctgc agacgtaccc gtcggcctac cgggcgggac gctgaccggg
      421 gacgcccccg ctggccccgc cgcagcgcat ccgtactaac gcagtccaga caaccatgcg
      481 tatcctgtcc ctcgccgccc tcctctccgc cctgctcgcc gccgcgcccg ccgcaacgcc
      541 ggccgccgcg caggatgccc ccctcttcac gcgcgacttc ccgcccgagg agttcgccca
      601 gcgacgcgaa cgggtctacg acgccatcgg ccccgacgcc gtggccatca tccagggcga
      661 gccctcaccc aggggctacg tccggttccg gcagtcgaac gagttctatt acctctccgg
      721 catcgaggtg ccgcacgcct acctgctcct cgacggcgcg aaccgccggg ccgtcgtgtt
      781 ccttccgcac cgtgacgcgc ggcgcgaatc gagcgagggc cggatgctct cggccgacga
      841 acccgacctg gtccgcgatc tttccggcat cgacgcggtg gcggccgcgg aggagctctc
      901 gaccgcgctc cacggcatgc ttcgccgcga ccgccttcca acggtctata ccctcttcaa
      961 gcccgccgag ggcctggccg agagccgcga cctgggggtc cggcggatca acgaccggct
     1021 cgcggacgcg tgggacggcc ggccgtcgcg cgaggcgcac ttcatgggcc tcctgcgcga
     1081 gcggctcccc ggcgtccgga tcgccgacct gcaccccatc ctggacgggc tgcgcctcat
     1141 caagagcccg cgcgagatgg agatgatccg cagggccacc cggctgtcgg tcgccgccat
     1201 cgaggaggct atgcgctccg tcgagccggg cgtcgtcgag cgcgaaatcg acgccctggc
     1261 caagttcatc tactttcgcg acggtgccca gggagacgcc tactattcgc tggtcgcgag
     1321 cggtccgaac gcctggtatc cgcactacca caagggcgcc cgcacgatgc aggcgggtga
     1381 gatggtcctg atggactacg cgcccgacgt cgggtactac atgtccgaca tcacccggat
     1441 gatgccggtc gacggccgct tcacggacgc acaccgggcc ctgtacggct tctacgagag
     1501 ggcctatcgc gccatcctgg accatatccg gccgggagcg accccgaacc agatcaaggc
     1561 cgaggccgtc gcggacatgc gggaggccct ctccggctgg acgttcgtcg aggagcacca
     1621 ccgggccgcc gccgaggcct tcgtggaggg ctacgcccag tccgcgcggg gcgagtacgg
     1681 gagcctgggg catttcgtcg ggatggcgac gcatgacgtg ggcacgatcg acggcccgct
     1741 gcgcgaaggc atggtcttca cgatcgagcc cgccctgcgc gtgcccgagg aacagctcta
     1801 catccggatg gaggacctca tcatcatcca cgctgaccgg gccgagatcg tctcgatcga
     1861 cctgcccatg tcggtggagg acatcgagaa cctgatggcg gagccggggc tgctgcaaac
     1921 ccggccgagt acggtcatgc ggtgagtcgc cgaggtgcgg ccagggctca gcgcttcatg
     1981 cgtctgcggc tcacgccgag gctccgcgcc tcacgcgtcc gcgccgaaat cgggtcggcg
     2041 catctcccgg acgcccagga ctccgagcgt cagcacggtg agcggcacca cgaaccagag
     2101 catcaccgtc accagctcgt cgatcggtga gttgccgatc gcggcagcca ccacgtacgc
     2161 cgtatacgcc acgtagtagg ccaggaagag ggctccctcc caccggtcga tccggtggcc
     2221 ggtgaagaag atcggcagac aggccaccgc ggccgcgatc acgatcggga agtcgaagcg
     2281 cagcacctct tcgctgaccg ccaggcctcc gggggcggcc accgccgcca cgcccaggac
     2341 gccggacagg ttgaacagat tgctgccaac cacgttgccc acggcgaggt cccgttctcc
     2401 gcggacgctc gccaggaccg aggtcgcgag ctccggtaac gacgtcccca gggcaaccac
     2461 cgtaagaccg atcgtcagct cgttcacccc gaggcggcga gcgatctcgg tcgccccgtc
     2521 gaccatgagg tccgagccca ggaccagaag tccgagcccc accgcgatca ggacgccgtt
     2581 gagcaggccg acctgccacc ggggtctttc cgtgggagcg gatgcgtcgg cgggcatcgc
     2641 cgcgacaagg gccgcgccaa ggctcgcatc ggccctgccc ttgcgcacca ggaacgccgt
     2701 gtaggccacg aggcctgcca tcagaaggac accctcggcc gcccccacgg tcccgttcga
     2761 agcgagcgcc cacacgccga tcgacagggc gaccacgacg ggcacgtcga gccggaccag
     2821 ctgccgggcg acgttcagcg gcgcgatgac ggccgagacc ccgaggatca gcagcacgtt
     2881 caggatgttg ctgccgagca cgttgcccac ggccaggtcg ccccgccccc cggccgccgc
     2941 gaccgcggaa accgccatct cgggcgcgct cgtcccaaac gcgactatcg tcagaccgat
     3001 gacgagcggc ggaacaccga agacggcggc gagccgagac gcgccgcgca cgagcagctc
     3061 ggcaccggcg acgaggagaa cgaggccgga ggcaaggaga agcaggggca tgcggcgagg
     3121 gctattcgtc ctccgtggct gtccgtgccg cgatggtcgc ggccggcgcg ggcgccgcgg
     3181 cgcgaacgcg cgccgcgccg agcagcacgc cgagagcgcc gaggaacggg ttgaggaaag
     3241 tgagccaccg gggctccacc gacatcgaca tggcatccga catgctcacg tctcccaagc
     3301 gcacggcgcc gacgccttcg gacgtccaca tgaacgtcat cacgacgccc agcaccagga
     3361 cgatgccggc gagcaccttc gcggcgagcc ggttgcgggc gagcacggtg cagacgtacc
     3421 cgccggcgta ggccgcgacg aagctcagga tgatgcttcc gatgatccag agggcggtaa
     3481 cctcgtaggt acccggctta taggtggcct cgacaccgat caggagatag tagacggtga
     3541 acaggaccat gacaccgatg aacatcgcgg cataaccggc gacgacggca aggacggtgc
     3601 ggagcatggg atcggagggt tggttcgggg atgccctgaa ttacgacgcg gcgcgcgatc
     3661 cgcgcaacgg gggcgcgcgc cgcggtcgtc tggcgggccg gtagcgcctg tgacggaacc
     3721 cgtcgcggcg taccttgcgg cttcagtggc cctgaacccg atgattctcc gcctccttcc
     3781 cgcgatcctg tcgctcgtcg tcctcggcgc ccacttcctg cgcctcggca acctcgtcgt
     3841 tgttgcactg ctcatcgcct gcatcggcct cctgtgggtg cgacgcccct gggcggccta
     3901 ttccgtgcag acgatcctgg ccatgggcat cgtggtgtgg gtcctgacgg gctacggcat
     3961 cgcgcgagtc cggattcaga acggcgagcc ctggatccgc atggcggtga tcctgggtgc
     4021 cgtcgccgcc atggccctat acgcgatcgt gagcttccgg cggccgcgca taagggcctg
     4081 gttcgggacg gaccagccgg gcgccgccga ccagccgacc gtcgagccgt cgtgacggcg
     4141 ccccgacgta ccttccggga acgcgcagac ctcaatgcct gagccgccgt cagaccggat
     4201 ctacaccgac gacgagatcg cccgcatcct caagcgtgcg gccgacctgc agggccggga
     4261 aagccgcagg cccgtgctcg gcctcaccct ccccgagctg cagcagctcg cccgcgagtc
     4321 ggacatcgac ccggcgctca tcaccgccgc cgccctggag cttgccggaa tccccgatca
     4381 ggaagaggcg ggattctggg gcggaccgct gaccgtcacc atccggcggc gcgtggaagg
     4441 cagcgtctcg gaggacgcct tcaatcgcat ggtgtcggag
//
LOCUS       DBNHQZ010000258        12706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1298609, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000258 DBNHQZ010000000
VERSION     DBNHQZ010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1298609"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="AC16YU_06920"
     CDS             <1..556
                     /locus_tag="AC16YU_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HIR6064993.1"
                     /translation="PEALRLAAFQAVSIVTTTGFGTADYEAWSPLAVGVLFVLFYIGG
                     MAGSTGGGVKVIRHVLMFKNSYKELRQLVHPQAVLTVRLGDQVVPQDVMRNVLSFIVL
                     YLGLIGFGTLGLAAGGVDLLSSFGGTISAVGNIGPAFGTLGPAENYAHLPDMAKWILS
                     FLMVAGRLEIFTVLIVFASAFWRR"
     gene            721..960
                     /locus_tag="AC16YU_06925"
     CDS             721..960
                     /locus_tag="AC16YU_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064994.1"
                     /translation="MERFARRIPGGIAWERDLFRRADTPPASELIDAAVDIAWECLTP
                     ENALEHPSLDIMERGALLVIRLSDARTQLDLPLAA"
     gene            complement(998..1531)
                     /locus_tag="AC16YU_06930"
     CDS             complement(998..1531)
                     /locus_tag="AC16YU_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6064995.1"
                     /translation="MPKKRTNPPPTTLLDYGPLEGGLVRMMDRFRDEGTMTGDPGADR
                     FLRADPNAALLGLLYDQRVRAEYAFTGPMRLRDRLGHLDMRRIARMDGQELRDAFSVL
                     PAVHRFTNRMADYTHGVALALVRDWKGSALALWDDGADFETIRRRVEALPGFGPQKAA
                     KMKYVLHYFGWRDFTGA"
     gene            complement(1581..3083)
                     /gene="cysS"
                     /locus_tag="AC16YU_06935"
     CDS             complement(1581..3083)
                     /gene="cysS"
                     /locus_tag="AC16YU_06935"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6064996.1"
                     /translation="MSNDPEFRLFNTLSRSVEPVMPAEPGHLRFYSCGPTVYSFAHIG
                     NFRSFLTADLILRTAHAIGWKTTYVTNITDVGHLTEDDAADAAGEDRMAKALQSKEGE
                     RFANVWDLARFYTGELMRDWHTLNLHEPSVRPRATEHMREQITAVERLIEKGHAYETE
                     QGIYFSVPSFEGYGKLSGNERADELEQAVRDVVADPGKRDPRDFALWKKDPKHLMQWY
                     SPWGWGFPGWHIECSVMAIEYLGEEIDLHAGGEDLIFPHHECEIAQAESLTGRPFARH
                     WVHTRFLQVEGEKMAKRTGNFFTVRDLVAPASEGGRGVDPLALRLALISGQYRKPFNF
                     TRDTLRASAKHVARFGEVVEAVRAASGDGPDLVGDRLRPLYDRALAAMLDDLNTPEAI
                     AAVIEGFKLLHGMRKHLNEASAAAARAWIDSVNALLGIVEHEREPAAPAGGDDDPERA
                     GIDALVAERQEARAARDFVRADAIRERLTALGVEVMDTSEGAVWRRVTAV"
     gene            3333..4619
                     /locus_tag="AC16YU_06940"
     CDS             3333..4619
                     /locus_tag="AC16YU_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6064997.1"
                     /translation="MKKFIALGIVLVATGATVWQLTKGDGKPTTSYRFVEVERGDLES
                     VVTATGTLGAVTNVQVGTQVSGIVEKLYVDFNDHVRRGQLLARIDTTLLASAVRDART
                     NLERNIAQRDYMQTELDRIRGLHERGFSTDVELNKAAYDLKIAVASVKTSEVSLERAE
                     RNLSYARIYSPIDGVVVERNVDEGQTVAASMNTPQLYLIANDLSRMQILASVDESDIG
                     RIREGQEVRFTVQAFDEETFAGIVRQVRLQSATQENVVTYTVVVDVDNSDGRLLPGMT
                     ATVEFLTDKVTDVFKVANAALRFRPTDEMMADFRARMEARREDRERTGDSTRAGGPGA
                     GFGMGGGFAGAAASGNAAGGASDRAMLWTVDEEGRLMIRPVRTGLTDGTMTEISGRDL
                     EVGLQIIAGVTTAEESASANPFQQGTRQFGPRPGGF"
     gene            4633..5382
                     /locus_tag="AC16YU_06945"
     CDS             4633..5382
                     /locus_tag="AC16YU_06945"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6064998.1"
                     /translation="MTDRIVIETHDLHKVYAMGENQVFALQGVDLTVRQGEMIAIMGA
                     SGSGKSTLMNIIGCLDYPTSGHYTLDGIRVDSLSPNALADIRNQKIGFVFQGFNLLAR
                     TSALENVELPLLYDRTGRKINVKQAAEAALDRVGLRDRMHHVPSELSGGQQQRVAIAR
                     ALVTEPAILLADEPTGNLDSRTSVEVMALFQELNDQGITILLVTHEHDVASYARRVVE
                     LRDGRVVRDIEQEQVRADEAMLHLDPESVIV"
     gene            5431..6648
                     /locus_tag="AC16YU_06950"
     CDS             5431..6648
                     /locus_tag="AC16YU_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6064999.1"
                     /translation="MKSSNLVKVAGRSIVRNKMRTALTMLGIIIGVGAVIVMVAVGDG
                     ARSRIEQQIQNLGTNMLVVTPGISTTGGVSRGAGTFNRLRLEDSEKLRNESMYLSAIS
                     PVVTTHGQIVGGSGNWRARVSGVDVDYVTIRDWRVESGSFFTAADVRQGKKVVVLGKT
                     VATQLFGESDPVGQTVRIRNVPVQVIGVMAPKGQTAEGSDQDDTILAPYTTVGTRLSR
                     WNFVSQILASTSSPNDIPLAQEEMKSIMREAHALASWEEDDFDVKNQNELTEAAAGTT
                     EVMTLLLAAIASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGARGRDVLTQFLIES
                     IVMSLFGGAIGVGLGFAGARILGNVMGWSTLVNAETVTIALVFSAAVGIFFGFYPARK
                     AARLNPIEALRYE"
     gene            7320..9473
                     /locus_tag="AC16YU_06955"
     CDS             7320..9473
                     /locus_tag="AC16YU_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6065000.1"
                     /translation="MVRNLRAGAILLVLAAAPAPVLAQHAVRALVVDAHDGGPIPGVH
                     VVVEGTTVGGITDGEGRIELPPLPAGRHAVRFSFVGFRTEIRSITIPGTDSLLVVALE
                     EDEMELAGAMVTATRSTRTIADTPTRVEVIGGEEIEEKIFMDPSGISMLLSESPGIVV
                     QQTSTVSAGATFRIQGLDGRYTQLLRDGFPLYGGLEGGLSLLQVPPLDLAQVEIIKGP
                     ASTLYGGDAIAGLVNLVSKQPNPAGVRSVLVNATSAGGLDGALYLAGRSQRLGWTLLA
                     SGNMQQPYDPESDRFTNLPWTRRLSLAPTLLRYGRGTLTVRLSGMVERRRGGDVDAVR
                     GDAPGVTERNASERVTAAVGYERPAGGSTRVTLRTSGSLFRRTLEVPDFRFEGRQVAS
                     YSEASLAYRRGSHDAVLGMDLRTDRFVQGAGGEPPLDHSHASIGLFAQDTWDVSPVVT
                     LEAGLRVDRRSRFGFIALPRVALLVRPGSGFTARVGGGLGYKEPTPFLEDAEARAYLG
                     VAPVPDDVGDERSQGLGVDLNYRASVGRLALALNQAAYVTRLERPLVAVADGALLTFE
                     SAEGHLLARGSETTARLSLGDLSLFLGYVYVDVSRETDGESVQLPLTARHRTYTVLVW
                     ERHGDSRVGFEAYYTGPQRLPGGGESPGFVVTGIMAQKRFGTVRVFANLENLLDTRQS
                     RTSPLVTGPRTSPVYEPVWGPTDGFVANAGFILDL"
     gene            complement(9457..9978)
                     /locus_tag="AC16YU_06960"
     CDS             complement(9457..9978)
                     /locus_tag="AC16YU_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR6065001.1"
                     /translation="MSVKKHRKDSADVRIRCAVITCSDTRTLDDDPSGDLIASLLEGA
                     GHEIVSRQLVIEHPDAIKLALDAGRIDADAVILTGGTGLATRDRTPDVIEPLLDRTLP
                     GFGELFRMLSFQEVGAAAMLSRAFAGVRERSVVFALPGSPAAVRLAMEKLILPELGHV
                     VGQVQGFTPTGPG"
     gene            complement(9975..10271)
                     /gene="yidD"
                     /locus_tag="AC16YU_06965"
     CDS             complement(9975..10271)
                     /gene="yidD"
                     /locus_tag="AC16YU_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6065002.1"
                     /translation="MLRALLRLPSRLLVGLVRLYQLVLSPHVGGGCRFTPTCSNYAIQ
                     ALERYGALKGTILAVHRILRCNPWGGHGYDPPVWYAERGRALPPDPASIDHEHP"
     gene            complement(10283..>12706)
                     /gene="dnaE"
                     /locus_tag="AC16YU_06970"
     CDS             complement(10283..>12706)
                     /gene="dnaE"
                     /locus_tag="AC16YU_06970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6065003.1"
                     /translation="GPGRGSAAGSLVAYSLGITDIDPLRYDLLFERFLNPERVSMPDI
                     DIDFDDRGRGRVIDYVVQKYGRENVCQIITFGTMGARSALRDVARVLGIPLADADRIA
                     KMVPEGVKVTLDKAIEDVPDFRALLKDPNEQIRNLMHYARVLEGSARHTGVHAAGVII
                     APGPVSDFVPVAVTKGKGDLDTVVTTQYDGKWVEEFGLLKMDFLGLKTLTVLDDTLRL
                     VEATRGERIDLDRIPLDDPRTFELFQRGDTVAIFQFESSGMREWLRKLRPTSIDDLIA
                     MNALYRPGPMDLIPNFIARKHGDEPVHYPHPMLEPVLEPTYGIPVYQEQVMQMAQVMA
                     GYTLAGADLLRRAMSKKKVSEMDRQRETFVKGAMERGVEEPTATEVFDMMAKFAGYGF
                     NKSHAAAYSIVAYQTAWLKANYAPEFLAAAMTNEMGDTKKLAVVLEDARHLGVEVLAP
                     SINRSEAAFGVDGGRILFGLGAIKGVGLGAIESIIEAREKHGPFPSLFDLAAAVDARA
                     VNRKVYESLIRAGALSELEGHRAQQMEALDAAVQYGIRLQADRAAGQNSLFGAPGGDG
                     AAVQAPQLPVVEAWPRARTLKEERDVVGFYVSGHPLEAYTPEVRAFASAHIAEIESIV
                     ASHDPSEDERGYNSRPVQTVCGIITDVQHRRSKAGKPMAFLSIEDVTGQAEVLCFSSV
                     YDRSQRYLEADEVVLVRGGLEVRGGSPKIIADEITPMWKVREQMVKSLVLDLDPRTLA
                     EESVNELHTLCEEHRGPVRLYFEVSLPGDGRQRILSRTVAVEADAELMQGLARLVGRH
                     RIQVEGERT"
BASE COUNT         1872 a         4305 c         4522 g         2007 t
ORIGIN      
        1 gccggaggcg ctgcgcctgg ccgcattcca ggccgtttcc atcgtgacga cgacgggttt
       61 cggcacggcc gactacgagg cgtggtcgcc cctggccgtc ggcgtgctct tcgtgctctt
      121 ctacatcggg ggcatggccg gctcgaccgg cggcggcgtg aaggtgatcc gccacgtgct
      181 catgttcaag aattcgtaca aggaactccg ccagctcgtc cacccgcagg ccgtgctcac
      241 cgttcggctg ggcgaccagg tggtgccgca ggacgtcatg cgcaacgtcc tgtcgttcat
      301 cgtcctgtac ctgggcctca tcggcttcgg gacgctgggc ctggccgcgg gaggcgtcga
      361 tctgctgtcg tcgttcgggg ggacgatctc ggccgtgggt aacatcggac cggccttcgg
      421 aacgctgggc cccgccgaga actacgccca ccttccggac atggccaagt ggatcctgtc
      481 gttcctcatg gtcgccggcc gcctggagat ctttaccgtg ctcatcgtgt tcgcctccgc
      541 cttctggcgc cgctgacacg cggccgaccg tgccgcccgg cggcaattcc ttcccgcccc
      601 ggaccggccc tgcccggggc aacatggcgt ttccggcccc tttgcggcgg atggcatgcc
      661 gattgtccgt atctgaggaa gccaccgtcc ggtgcactcc tcatgaccgc ccgcgagacc
      721 atggaacgct tcgcccgccg gatccccggc gggatcgcct gggagagaga tctgttccgc
      781 cgcgccgata cgccgccggc gtcggagctg atcgatgccg cggtcgacat cgcgtgggag
      841 tgcctgaccc cggagaacgc gctggagcat ccctcgctcg acatcatgga gcgcggagcg
      901 ctgctcgtga tccggctgtc cgacgcgcgg acgcaactgg acctgccgct ggcggcctga
      961 gcggccgccg acgtgccgcg ccgcgccgcg gtcggcctca ggcgccggtg aagtcgcgcc
     1021 agccgaagta gtggagcacg tacttcatct tggcggcctt ctgcggcccg aagcccggca
     1081 gcgcttcgac gcggcgccgg atggtctcga agtcggcccc gtcgtcccac agcgcgagcg
     1141 ccgacccctt ccagtcccga acgagcgcca gggccacgcc gtgcgtgtaa tcggccatcc
     1201 ggttcgtgaa gcggtgaacg gccgggagca cggaaaaggc gtcgcgaagc tcctggccgt
     1261 ccatgcgggc gatcctgcgc atgtccaggt gacccagccg atcgcgcagg cgcatcggac
     1321 ccgtgaaggc atactccgcg cggacccgct ggtcatagag caggcccagg agggcggcgt
     1381 tcgggtcggc acgcaggaag cggtccgcgc cggggtcgcc cgtcatcgtg ccctcgtcgc
     1441 ggaaccggtc catcatgcgg accaggccgc cctccagcgg cccgtagtcg aggagcgtgg
     1501 tcggcggggg attcgttcgt ttcttcggca cggcgggtgc gttccggatc gtgaggagta
     1561 cacgtgatgg agggcgcgag tcagacggcc gtaacgcgtc tccagacggc gccctcggag
     1621 gtgtccatca cctcgacgcc cagggccgtg agccgctcgc ggatcgcgtc ggcgcgcacg
     1681 aagtcgcggg cagcgcgggc ctcctggcgc tcggcgacca gggcgtcgat cccggcgcgc
     1741 tcgggatcgt cgtcgccccc ggcaggcgcg gccggctcgc gctcgtgctc gacgatgccg
     1801 aggagggcgt tcacagagtc gatccaggcc cgcgcggccg ccgccgaggc ctcgttcagg
     1861 tgctttcgca ttccgtgcag cagcttgaaa ccttcgatca cggccgcgat cgcctcgggg
     1921 gtgttcaggt cgtcgagcat cgccgcgagc gcccggtcgt agagcggccg cagccggtcg
     1981 cccacgaggt cggggccgtc gccggaggcc gctcggaccg cctcgaccac ctcgccgaac
     2041 cgcgcgacgt gcttcgcgct cgcccgcagc gtgtcccgcg tgaagttgaa cggcttgcgg
     2101 tactgccccg agatgagcgc gagccggagg gccagcgggt ccacgccgcg gccgccctcg
     2161 gaggcgggtg cgaccaggtc gcgcacggtg aagaagttgc ccgtgcgctt ggccatcttc
     2221 tcgccttcga cctgcaggaa gcgcgtgtgc acccagtggc gggcgaacgg ccggcccgtc
     2281 aggctctcgg cctgcgcgat ctcgcactcg tggtgcggga agatgaggtc ctcgccgccc
     2341 gcgtgcaggt cgatctcctc tccgaggtac tcgatcgcca tcaccgagca ctcgatgtgc
     2401 cagcccggga agccccatcc ccaggggctg taccactgca tgaggtgctt cgggtccttc
     2461 ttccacaggg cgaagtcccg gggatcccgc ttgccgggat cggccaccac gtcacgcacg
     2521 gcctgctcca gctcgtccgc ccgttcgttt cccgacagct tgccgtaccc ctcgaagctc
     2581 gggaccgaga agtagatgcc ctgttcggtc tcgtaggcgt gccccttctc gatgagccgt
     2641 tcgaccgccg tgatctgctc gcgcatgtgc tcggtggccc ggggcctgac cgacggctcg
     2701 tgcaggttca gcgtgtgcca gtcgcgcatg agctccccgg tgtagaagcg cgcgaggtcc
     2761 cagacgttgg cgaagcgctc gccttccttc gactgcaggg ccttggccat gcggtcctcg
     2821 cccgcggcgt cggccgcgtc gtcctcggtc aggtggccga cgtcggtgat gttcgtcacg
     2881 taggtcgtct tccagccgat ggcgtgcgcg gtcctcagga tcaggtccgc ggtcaggaac
     2941 gagcggaaat tcccgatgtg ggcgaacgag tagacggtcg gcccgcacga gtagaaccgg
     3001 agatgcccgg gctccgcggg catcaccggc tcgaccgatc gcgaaagcgt gttgaagagg
     3061 cggaactcgg ggtcgttcga catggcgggt catcctgtgg caggggcacg aacgccgttg
     3121 tcggggacgg gttcgtcgcg gcgcggggcc cgcggtgtgc aggtcccgtg aaggcgcggc
     3181 ggtcgcccgg tcaccgaatt gtcacgtttc tattccattt ggccggggga cccgcgagcc
     3241 cgaaacggag gtcgtcgccg gggcgtattc gcggcaggag aagagaacac ccccattacg
     3301 gatatcatcc tgcctccggg caggattcca tcatgaagaa gttcatcgca ttgggcatcg
     3361 tcctcgtcgc gacgggcgcc accgtgtggc agctgacgaa gggcgacggc aagccgacca
     3421 cgagctaccg gttcgtggag gtcgagcgcg gcgacctcga gtccgtggtc accgccaccg
     3481 gcacgctcgg cgccgtaacc aacgtgcagg tcggcaccca ggtgtcgggc atcgtcgaga
     3541 agctgtacgt ggacttcaac gaccacgtcc ggcgcggcca gctcctggcc cggatcgaca
     3601 cgacgctgct cgcctcggcc gtccgggacg cccgtacgaa cctcgagcgc aacatcgccc
     3661 agcgcgacta catgcagacg gaactggacc ggatccgggg gctccacgag cggggcttct
     3721 cgacggatgt cgagctgaac aaggcggcgt acgatctgaa gatcgccgtg gcgtccgtca
     3781 agacctccga ggtcagcctg gagcgggccg agcggaacct gtcgtacgcc cgaatctact
     3841 cgcccatcga cggcgtggtc gtggagcgca acgtggacga ggggcagacc gtggccgcca
     3901 gcatgaacac cccgcagctc tatctcatcg cgaatgacct ttcccgcatg cagatcctgg
     3961 cctcggtcga cgagagcgac atcggacgga tccgcgaggg gcaggaggtg cgcttcaccg
     4021 tgcaggcctt cgacgaggag actttcgccg gcatcgtccg ccaggtgcgg ctgcagtcgg
     4081 ccacgcagga gaacgtcgtg acctacacgg tggtcgtcga cgtcgacaac tcggacggac
     4141 ggcttctccc cggcatgacg gcgaccgtgg aattcctcac cgacaaggtg accgacgtct
     4201 tcaaggtggc caacgcggcg ctccgcttcc ggccgaccga cgagatgatg gcggatttcc
     4261 gcgcccgcat ggaagcacgg cgcgaggatc gggagcgcac gggcgattcg acgcgggccg
     4321 gagggcctgg cgcgggcttc gggatgggcg gcggctttgc cggcgcggcg gcgagcggga
     4381 acgcggcggg cggagcgtcc gaccgcgcga tgctctggac ggtggacgag gagggacggc
     4441 tgatgatccg gccggtccgg accgggctga cggacgggac gatgaccgag atctcgggac
     4501 gcgacctgga ggtcggcctg cagatcatcg ccggggtcac gaccgcggag gagtccgcgt
     4561 cggccaaccc gttccagcag ggcacacggc agttcggacc ccgtccgggc ggattctgac
     4621 ccggcaccga gcatgaccga ccgaatcgtc atcgagacgc acgacctgca caaggtctac
     4681 gccatgggag agaaccaggt cttcgccctg cagggcgtgg acctgaccgt ccggcagggg
     4741 gagatgatcg ccatcatggg cgcgtccgga tccggcaagt cgaccctcat gaacatcatc
     4801 ggctgcctgg actacccgac ctccggccac tacacgctcg acggcatccg ggtcgacagc
     4861 ctttcgccga acgcattggc ggacatcagg aaccagaaaa tcgggttcgt cttccagggc
     4921 ttcaacctgc tcgcccggac gtcggccctg gagaacgtcg agctgccgct gctctacgac
     4981 cgcaccgggc gaaaaatcaa cgtgaaacag gcggccgagg ccgctctcga tcgcgtgggg
     5041 ctacgcgacc ggatgcacca cgtgccgagc gagctctcgg gtgggcagca gcagcgcgtg
     5101 gcgatcgccc gtgcgctcgt cacagagccc gcgatcctcc tcgcggacga gccgaccggc
     5161 aacctggaca gccgcacgtc cgtcgaggtc atggcgctct tccaggagct caacgaccag
     5221 ggcatcacca tcctcctcgt cacgcacgag cacgacgtgg ccagttacgc gcgccgcgtg
     5281 gtcgagctgc gagacggccg ggtggtccgc gacatcgagc aggagcaggt ccgcgccgat
     5341 gaggccatgc tgcacctgga tccagaaagc gtcatcgtgt agaccgcgcc gcccccaggc
     5401 cgccagccac acgcccaccc ccggaacgtc atgaaatcat cgaacctcgt caaggtcgcc
     5461 ggccgcagca tcgtccggaa caagatgcgc acggcgctca cgatgctggg catcatcatc
     5521 ggcgtgggcg cggtgatcgt catggtggcc gtgggcgacg gcgcccgctc gcggatcgag
     5581 cagcagatcc agaacctggg caccaacatg ctggtggtga cgccgggcat cagcacgacc
     5641 gggggggtga gccggggcgc ggggacgttc aaccggctca ggctcgagga ctccgagaag
     5701 ctccggaacg agtccatgta cctgtcggcg atctcgcccg tcgtgaccac ccacgggcag
     5761 atcgtgggcg gcagcggaaa ctggcgggcg cgcgtgagcg gcgtggacgt ggactacgtc
     5821 acgatccgcg actggcgggt ggaatcggga tcgttcttca cggcggccga cgtccggcag
     5881 ggcaagaagg tggtcgtcct cggcaagacc gtggcgacgc agctcttcgg cgagtcggac
     5941 cccgtggggc agaccgtccg catccggaac gtgccggtcc aggtgatcgg cgtgatggcg
     6001 cccaaggggc agacggccga gggcagcgac caggacgaca ccatcctggc cccgtacacg
     6061 accgtcggaa cccgtctgag ccgctggaac ttcgtctccc agatcctggc gtccacctcg
     6121 tcaccgaacg acattccgct cgcccaggaa gagatgaagt cgatcatgcg cgaggcgcac
     6181 gcgctcgcct cgtgggagga ggacgacttc gacgtgaaga accagaacga gctcaccgag
     6241 gccgccgccg gcaccaccga ggtgatgacg ctgctcctcg cggccattgc gtctatctct
     6301 ctcctggtgg ggggcatcgg catcatgaac atcatgctcg tctccgtgac cgagcgcacg
     6361 cgcgagatcg gcatccggat ggcgatcggc gcgcgcggcc gcgacgtgct gacccagttc
     6421 ctgatcgagt cgattgtgat gtcgctcttc ggcggcgcga tcggcgtggg tctcggcttc
     6481 gcgggcgccc gcatcctggg gaacgtgatg gggtggagca cgctcgtgaa cgcggagacc
     6541 gtgacgatcg cgctcgtgtt ctcggcggcg gtcgggatct tcttcgggtt ctacccggcg
     6601 cggaaggcgg cgcggctgaa tccgatcgag gcgctgcgtt acgagtaggt cgaggcggtt
     6661 gccgggaatg ctccgggact ggcccggggg cgccggagcc gattgggcgc tgttcgccgg
     6721 gccattcagt ccgcttcccg gcaaccgcct cgaccacctc ggtggtgggc tcgggggttg
     6781 tgggcgccgg ccgcttcgcg gcgaccgtct cgattacgtc ggtggtgggc tcgggggttg
     6841 tgggcgccgg cggggcgagt gggggtgggg gaagcggtcc cggccgcgac ctatattgcc
     6901 tttgtgcgcc ccctattacg tctcagtccg tttcttgcct gcgttctcgc gggactcgtt
     6961 gctgcgcaga cggcggatct actgccctgt gcggacgacg gctgcggcgt atggaacgtg
     7021 gtgtcggatt ccgaccacga ggaccaggga cgcgctgggg aaggatcgcg gccggacgct
     7081 ccgtgttgcc tgtgcagggc ggatcttgcg acgtcgtccg tggccccggt cccgggatgg
     7141 atccgggtcg atgaagggat gagtgcggtt gacggcgtgc gtgcggtctc cggaccagca
     7201 cggcgcgtcc cccatccgcc tccgcgcggc tgacgagcgt ccgccgtccg ccaactccgt
     7261 cgctctcctc ctggcaccgc ctcaccccgc tgcgggggag gtccatcccg cacgtttcca
     7321 tggtccgcaa cctccgggcc ggcgcgatcc tgttggttct cgccgccgcc cccgctcccg
     7381 ttctcgcgca gcatgcggtc cgcgcgctcg tcgtcgacgc ccacgacgga gggccgattc
     7441 ccggcgtgca cgtcgtcgta gaagggacga ccgtgggagg catcacggac ggggaggggc
     7501 gaatcgaact tccgccgctg cccgccggcc gccacgcggt ccggttctcg ttcgtagggt
     7561 tccgcaccga aatccgaagc atcacgattc cagggacgga ctcgctcctc gtggtggccc
     7621 tggaggagga cgagatggaa ctcgccggcg cgatggtgac cgccacccgg tcgacgcgga
     7681 ccatcgcgga cacgccgacg cgcgtcgagg tcatcggtgg cgaggagatc gaggagaaga
     7741 tcttcatgga tccgtccggc atctcgatgc tgctcagcga gtccccgggg atcgtcgtgc
     7801 agcagacgtc gaccgtctcg gccggcgcca cattccgcat ccagggcctc gacggccgct
     7861 acacccagct gctgcgggac ggctttcccc tgtacggcgg cctcgagggc ggtctgtccc
     7921 tgctccaggt cccgccgctg gacctggcgc aggtggagat catcaagggc cccgcgtcca
     7981 cgctctacgg cggcgatgcg atcgcggggc tggtcaacct ggtctcgaag cagccgaacc
     8041 ctgccggcgt gcgctccgtc ctggtcaacg ccacgagcgc cggagggctc gacggcgcgc
     8101 tgtacctcgc ggggcggtcg cagcgcctgg gctggaccct cctggcctcg ggcaacatgc
     8161 agcagcccta cgacccggag agcgaccggt tcacgaacct cccctggacc cggcgcctga
     8221 gcctggcgcc cacgctcttg cgctacgggc gcggcacgct caccgttcgc ctgtccggca
     8281 tggtggaacg gcgccgcggc ggcgacgtcg acgccgtgcg cggcgatgcc cccggagtga
     8341 ccgagcggaa cgcgtccgag cgtgtgaccg cggccgtcgg ctacgagcgg cctgcgggag
     8401 gatccacgcg cgtgaccttg aggacgagcg gcagcctgtt ccggcgcacg ctcgaggttc
     8461 cggacttccg gttcgagggg cgccaggtcg cctcgtattc cgaggcgtcg ctcgcgtatc
     8521 gccgcggaag ccacgacgcg gtcctcggga tggatctccg gaccgaccgg ttcgtccagg
     8581 gggcgggggg agagccgccg ctggaccact cgcatgcgtc gatcgggctg ttcgcccagg
     8641 acacctggga tgtctccccg gtcgtaacgc tcgaggccgg cctgcgcgtg gatcgccgaa
     8701 gtcgtttcgg attcatcgcg ctcccgcgcg tggccctgct cgtgcgtccg ggctccggct
     8761 tcaccgcccg ggtcggggga gggctcgggt acaaggagcc cacgcccttc ctggaggatg
     8821 ccgaggcccg ggcgtacctg ggcgtggcgc ccgtaccgga cgacgtcggg gacgagcggt
     8881 cccaggggct gggtgtcgac ctgaactacc gcgcgagcgt cgggcgcctc gccctcgcgc
     8941 tgaaccaggc ggcatatgtc acgcgcctgg agcgtcccct ggtggcggtg gccgacggcg
     9001 cgctcctcac cttcgagagc gcggagggcc acctgctcgc ccgcggctcg gagacgaccg
     9061 cgcggctttc gctcggcgac ctgagtctct tcctcggata cgtgtacgtg gacgtttccc
     9121 gcgaaacgga cggggagtcg gtccagcttc cgctcaccgc gcggcaccgc acgtataccg
     9181 tgctggtctg ggagcgccac ggcgacagcc gcgtcgggtt cgaagcctac tacacgggcc
     9241 cgcagcgcct tcctggcggc ggagaatctc ccggcttcgt cgtcaccggg atcatggcgc
     9301 agaagcgctt cgggaccgtc cgggtgttcg cgaacctcga gaatctcctg gacacccgcc
     9361 agagccggac ctcgccgctc gtgaccggcc cgcgcacgtc gccggtctac gagcccgtct
     9421 ggggtccgac cgacggcttc gtcgcgaacg ccggtttcat cctggacctg taggcgtgaa
     9481 cccctgcacc tgccccacca cgtgcccgag ctcgggcagg atcagctttt ccatggcgag
     9541 ccggaccgcg gccgggctgc cgggtagggc aaagaccacc gaccgctccc gcacgcccgc
     9601 gaacgcgcga gagagcatgg ccgcggcgcc gacttcctgg aacgagagca tgcggaagag
     9661 ttcgccgaag ccgggcagcg tgcggtccag gagcggttcg atgacgtccg gcgtccggtc
     9721 gcgcgtggcc aggccggtgc cgccggtgag gatgacggcg tcggcgtcga tgcggccggc
     9781 gtcgagtgcg agcttgatgg cgtcggggtg ctcgatgacg agctggcgcg aaacgatctc
     9841 gtggcccgcg ccctcgagca gcgaagcgat gaggtcgccc gacggatcgt cgtcgagcgt
     9901 gcgcgtgtcg ctgcaggtga tgacggcgca gcggatccgg acgtcggctg aatccttcct
     9961 gtgcttcttg acgctcatgg gtgctcgtgg tcgatcgatg cggggtcggg cggaagggcc
    10021 cggccgcgct ccgcatacca cacgggcggg tcgtagccgt ggccgcccca cgggttgcat
    10081 cgaaggatac ggtgcacggc caggatggtg cccttcaggg ccccgtagcg ctccagggcc
    10141 tggatcgcgt agttcgagca ggtgggcgtg aagcggcacc cgccgccgac gtgaggcgaa
    10201 agaacgagct ggtacaggcg gacgagcccg acgagcaggc gggagggcag tcggaggagg
    10261 gcgcggagca tgggggtcgc ggtcaggtcc gctcgccctc gacctggatc cggtggcggc
    10321 cgacgaggcg ggccaggccc tgcatgagct cggcgtcggc ctcgacggcc acggtccggc
    10381 tcaggatgcg ctggcgtccg tcgccgggaa gggacacctc gaagtagagc cgcaccggcc
    10441 cccggtgctc ctcgcacagg gtgtggagct cgttgacgga ctcctcggcg agcgtccgcg
    10501 ggtccaggtc cagtacgagc gacttcacca tctgctcgcg gaccttccac atgggcgtga
    10561 tctcgtcggc gatgatcttg gggctgccgc cgcgcacctc gagtccgccg cgcacgagga
    10621 ccacctcgtc ggcctccagg tagcgctgcg agcggtcgta gacggaggag aagcagagca
    10681 cctcggcctg gccggtgaca tcctcgatcg agaggaaggc catgggcttg ccggccttcg
    10741 agcgccggtg ctggacgtcg gtgatgatcc cgcagaccgt ctgcacgggg cgcgagttgt
    10801 agccgcgttc gtcctccgaa ggatcgtgcg aggcgacgat ggactcgatc tcggcgatgt
    10861 gcgcggacgc gaaggcgcgc acctcgggcg tgtacgcctc cagcgggtgc ccggagacgt
    10921 agaagccgac gacgtcccgc tcttccttca gcgtccgcgc cctgggccag gcctcgacga
    10981 cggggagctg gggcgcctgg acggccgcgc cgtcgccgcc gggcgcgccg aagagcgaat
    11041 tctggccggc ggcccggtcg gcctgcaggc ggatgccgta ctggacggcg gcgtcgagtg
    11101 cctccatctg ctgcgcccgg tggccttcga gctcggacag cgcgcccgcg cggatgagcg
    11161 actcgtacac cttcctgttc acggcgcggg cgtccacggc ggcggcgagg tcgaagagcg
    11221 acgggaaggg cccgtgcttt tcgcgggcct cgatgatgct ctcgatggcg ccgaggccca
    11281 cacccttgat ggcgcccagg ccgaagagga tccgcccgcc atcgacgccg aaggcggcct
    11341 cgctccggtt gatcgacggt gcgagcacct cgacgcccag gtggcgggcg tcctccagga
    11401 ccacggcgag ctttttcgtg tcgcccattt cgttggtcat cgcggcggcg aggaactccg
    11461 gcgcgtagtt ggccttgagc caggcggtct ggtacgcgac gatggagtag gcggccgcgt
    11521 gcgacttgtt gaagccgtat cccgcgaact tggccatcat gtcgaagacc tcggtcgccg
    11581 tcggctcctc cacgccgcgc tccatggcgc ccttcacgaa ggtctcgcgc tggcggtcca
    11641 tctcggagac cttcttcttg ctcatggccc gtcgcaggag gtcggcgccg gcgagcgtgt
    11701 agccggccat gacctgggcc atctgcatca cctgctcctg gtagaccggg atgccgtagg
    11761 tcggctcgag cacgggctcg agcatggggt gcgggtagtg gacgggctcg tccccgtgct
    11821 tgcgcgcgat gaagttgggg atgaggtcca tgggccccgg gcggtacagg gcgttcatgg
    11881 cgatcaggtc gtcgatcgag gtcggccgca gcttgcgcag ccactcccgc atgccggacg
    11941 actcgaactg gaagatggcg accgtgtcgc ccctctggaa gagctcgaac gtccgcggat
    12001 cgtcgagcgg gatgcggtcc aggtcgatcc gctcgccgcg cgtcgcttcg accaggcgca
    12061 gggtgtcgtc gaggacggtg agcgtcttga gcccgaggaa gtccatctta aggagcccga
    12121 actcctccac ccacttgccg tcgtactgcg tggtgacgac cgtgtcgaga tcgcccttgc
    12181 ccttcgtcac ggcgaccggc acgaagtcgc tcacggggcc cggcgcgatg atgacgccgg
    12241 ccgcgtgcac cccggtgtgc cgcgccgatc cctcgagcac ccgcgcgtaa tgcatgaggt
    12301 tgcggatctg ctcgttcggg tccttgagca gcgcgcggaa gtcggggacg tcctcgatgg
    12361 ccttgtcgag cgtcaccttg acgccctcgg gcaccatctt cgcgatccgg tcggcgtcgg
    12421 caagcggaat ccccagcacc cgcgcgacgt cgcgcagggc cgatcgggcg cccatcgtgc
    12481 cgaaggtgat gatctggcag acgttctcgc gcccgtactt ctgcacgacg tagtcgatca
    12541 cgcggccgcg cccgcggtcg tcgaagtcta tgtcgatgtc gggcatcgac acgcgctccg
    12601 ggttcaggaa gcgctcgaac aggagatcgt agcgcagggg atcgatgtcc gtgatcccga
    12661 ggctgtacgc gaccaggctg ccggccgccg agccgcggcc gggccc
//
LOCUS       DBNHQZ010000259         6056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3192760, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000259 DBNHQZ010000000
VERSION     DBNHQZ010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3192760"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC16YU_06975"
     CDS             complement(<1..559)
                     /locus_tag="AC16YU_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR6065004.1"
                     /translation="MRPVFRFVRHLADGVAMGVANLIPGVSGGTIALVLGIYERLIAA
                     TSDAVRSGMSLVRLDFRQGMRGFGALPWAFLVPILVGMIGTPLLAAGWLEGQLEQRPE
                     VMRGIFLGLVAGSLPVPWVRMRRRGMDLRLFAVAAAVAAWFLVGLPPRTVLSPTDFQV
                     FISAILAISAMVLPGVSGSFLLLVLG"
     gene            complement(631..2037)
                     /locus_tag="AC16YU_06980"
     CDS             complement(631..2037)
                     /locus_tag="AC16YU_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065005.1"
                     /translation="MFNFEFDDYDEFRDEDRLRELVDSFEAGDRAGYLDSDALDDIAS
                     WYYDQGRFEDALRVVDRVLANHPFSSDAWMRKGILLNNLDRHTDALDAYERALALNPV
                     DVETLVNRGITLDTLDRPKDALDSYRQALTLDPVNDEALFNIGVTMERLNQPAEAVSV
                     FERCADLNAEHPEVWYELGYCYDRTGEHARAVEAYDRHLDTDPYSHDAWYNRGIVLNK
                     MGRYDEAVQSYDMAIVIADDFASAYYNRGNAKANLGLLDEAVENYLQVIEIEGPDAAT
                     CYNIALAYEEQRKYREAIRYFEQAVEADETYGEAWYGLGCCFDALERFDEALVFFNRA
                     VVLNPGTADFWYAKADCAYTLGRIGEALAAYRTVVHLSPESRDAWVDYAETLFEARRP
                     QDALEAFRRAASLEPGNAGTYFQQAKALFALGRREESVRSLKTAFLLDPSKRDEFAHA
                     YPEMYGDIRIRRLLDIDG"
     gene            2237..2848
                     /gene="tmk"
                     /locus_tag="AC16YU_06985"
     CDS             2237..2848
                     /gene="tmk"
                     /locus_tag="AC16YU_06985"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6065006.1"
                     /translation="MLVSFEGIDGSGKSTQVERIRGRLLGSGQRVEVLREPGGTIVSE
                     RIRDLLLDDVLRVEPFAELLLFSAARAQLVSERIRPLLDEGCIVVCDRFFDSTTAYQG
                     GGRGLADPAWLDAFHRRVTGGLVPDRTYLLVVSLDVGAARRTDRPADRMEQAGAAFLE
                     RVQAAYRDLADAEPDRILLLDGDADPDAITARIWSDLEPRLPR"
     gene            2905..3387
                     /locus_tag="AC16YU_06990"
     CDS             2905..3387
                     /locus_tag="AC16YU_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR6065007.1"
                     /translation="MAFATADQLEEVHRLFAEFLHERKQRRTPERMAVLDAVYSADGH
                     LDADALYARLRDGDVHVSRATVYNTLELLSECDLVTRHQFGNKQAKYERSHSFRQHDH
                     LICMECGELFEFCDPRLQSVQEMVAEVFGFEISHHSLHIYGHCRRKDCPTRQPGADGS
                     "
     gene            3481..3885
                     /locus_tag="AC16YU_06995"
     CDS             3481..3885
                     /locus_tag="AC16YU_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065008.1"
                     /translation="MKRLTLIPLAVLLAAAAFPYGVRIESFDVSPEGSAFVLRWQTDI
                     EDAVDHFEVLRKTPGSNDEFVRVHEVESHGIGKPYVFRDDQVFKVGAEQLVYQLEAVF
                     DNGTRQVLHVQALNYTSTAVRRTWGSIKAMFQ"
     gene            3936..4448
                     /gene="pyrR"
                     /locus_tag="AC16YU_07000"
     CDS             3936..4448
                     /gene="pyrR"
                     /locus_tag="AC16YU_07000"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR6065009.1"
                     /translation="MARTRIMDAGQVGRTVRRLAFEILERNRGVENVVLVGIRERGAR
                     LSELLAEALQRVEKQTVPTWTLDVKAYRDDRDPAVPIPAPSDGPDLADRDVVLVDDVL
                     FTGRTARAALDAVVRFGRPRSIQLAVLVDRGHREYPIQADFVGRAIPTRHTERVVVDL
                     GDDPSVAVEE"
     gene            complement(4451..5293)
                     /locus_tag="AC16YU_07005"
     CDS             complement(4451..5293)
                     /locus_tag="AC16YU_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR6065010.1"
                     /translation="MPIDPDSRNGRGLVRSVVSRARGGLRALVAILRNRYFLVGIAVM
                     AVIGIATYLVVDRAIMPSFTRYDVSIVVPDVMTLPFDEAAGTLAASGLTAEEYVLRKP
                     NLPRDVVIDQRPPAGSRVKPGRRIYLTVNTGDTTTVTVPRVIGLSLREAQSRIVVLGL
                     TVPEILPDSIPASHANTVTRQQPGPGARVPHGADVTLWYSTGLGEAYVEVPDVTGLTP
                     DDARARLLSLRLRSVVLGEPDAEEPLVLAQSPAPGTQVREGFEVRLRVSEPPGDAPPE
                     EEEQ"
     gene            5652..>6056
                     /locus_tag="AC16YU_07010"
     rRNA            5652..>6056
                     /locus_tag="AC16YU_07010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1007 a         1975 c         2076 g          998 t
ORIGIN      
        1 tgccaagcac caggagcagg aaggagccgc tcacgccggg cagcaccatg gcgctgatgg
       61 cgaggatcgc ggaaatgaac acctggaagt cggtcggact caggaccgtt cgcgggggca
      121 ggcccacgag gaaccaggcc gcgacggccg ccgcgacggc gaagagccgc aggtccatcc
      181 ctcggcggcg catgcgcacc cagggcacgg gcagcgagcc ggcaacgagt ccgagaaaga
      241 tgccgcgcat gacctccggc ctctgttcga gctggccctc gagccacccg gccgccagca
      301 gcggagtgcc gatcatcccg acgaggatcg gcaccaggaa tgcccacggg agggcgccga
      361 acccccgcat cccctggcgg aagtccagtc ggacgagcga catccccgac cggacggcat
      421 cgctcgtcgc ggcgatcagc cgctcgtaga tgcccaggac cagcgcgatg gtgccgccgc
      481 tgacgcccgg aatgaggttt gccacgccca tggcgacgcc atcggccagg tggcggacga
      541 atcggaatac cgggcgcatg cggggtcaaa aagcatgcgg cggtccggga gcccggaccg
      601 ccgcacggag gggtgttttc cgggtgtctc tcagccgtcg atgtcgagga gccgccggat
      661 gcggatgtcc ccgtacatct ccggataggc gtgcgcgaac tcgtcccgct tggacggatc
      721 cagcaggaat gcggtcttga gcgagcgtac gctttcttcc cgccggccga gcgcgaagag
      781 cgccttggcc tgctggaaat aggtgccggc gttgccgggc tccaggctcg cggcccggcg
      841 gaaggcctcg agcgcgtcct gtgggcggcg ggcctcgaac agggtctcgg catagtccac
      901 ccaggcgtcg cggctctcgg gcgagaggtg cacgacggtc ctgtaggccg ccagcgcttc
      961 cccgatccgt ccgagagtgt aggcgcaatc cgccttcgcg taccagaagt ccgccgtgcc
     1021 aggattcagg acgacggccc ggttgaagaa gaccagcgcc tcgtcgaacc gctcgagcgc
     1081 gtcgaagcag cagccgagcc cgtaccaggc ttcaccgtac gtctcgtccg cctcgacggc
     1141 ctgctcgaag tagcggatgg cctcgcggta cttcctctgc tcctcgtagg cgagcgcgat
     1201 gttgtagcag gtggcggcgt cagggccctc gatctcgatg acctgcaggt agttctcgac
     1261 ggcctcgtcc agcagtccga gattcgcctt ggcgttgccg cggttgtagt aggccgaggc
     1321 gaagtcgtcg gcgatgacga tcgccatgtc gtacgactgg acggcctcat cgtaccgccc
     1381 catcttgttc agcacgatgc cgcggttgta ccaggcgtcg tgcgagtacg gatcggtgtc
     1441 caggtgacgg tcgtacgcct cgacagcgcg cgcgtgctcg cccgtgcggt cgtaacagta
     1501 gccgagctcg taccagacct ccgggtgctc cgcgttcaga tccgcgcagc gctcgaagac
     1561 ggagacggcc tcggcgggct ggttcaaccg ctccatcgtg acgccgatgt tgaacagcgc
     1621 ctcgtcgttg accgggtcca gcgtcagggc ctgccggtac gagtcgagcg cgtccttcgg
     1681 gcggtcgagc gtgtcgagcg tgatgccccg gttcaccagc gtctcgacgt ccaccggatt
     1741 cagggccagg gccctttcgt aggcgtcgag ggcatcggtg tgccggtcga ggttgttcag
     1801 caggatgccc ttgcgcatcc acgcgtcgga cgaaaagggg tggttcgcga gcacccggtc
     1861 cacgacgcga agggcatcct cgaaccgccc ctggtcatag taccagctcg cgatgtcgtc
     1921 cagcgcatcg gagtccaggt agccggcccg atcgccggct tcgaacgaat cgaccagctc
     1981 ccgaagccgg tcttcgtccc tgaattcgtc gtagtcgtcg aattcgaagt tgaacatgct
     2041 cccccgcctg gcttgatcgc cgtcccgatc gagcggactc cggggtccgc cctgacggga
     2101 cgtccgatga caagaatacg aaacgacgga gcactcgaaa agccgaagct atcgggttcc
     2161 gcgtacctct cgcctacagg gaacgcgcaa atcgtagatt gtcgcgcacg cccagccctt
     2221 tcgccgcctt ttctccatgc tagtatcgtt cgaaggaatc gacggaagcg ggaagagcac
     2281 gcaggtcgag cggatccgcg ggcgcctcct gggctccggc cagcgggtag aagtccttcg
     2341 cgagccgggc ggcacgatcg tttcggagcg gatccgcgat ctcctcctcg acgacgtgct
     2401 gcgcgtcgaa cccttcgccg agctcctgct cttcagcgcc gcccgtgcgc agctcgtgtc
     2461 ggagcggatc cgtccgctcc tcgacgaggg gtgcatcgtc gtgtgcgacc ggttcttcga
     2521 ctccacgacg gcctaccagg gcggcgggcg gggcctggcg gatccggcct ggctcgacgc
     2581 cttccaccgg cgtgtgaccg ggggactggt cccggaccgg acgtacctgc tcgtcgtgtc
     2641 tctggacgtg ggtgccgccc gcaggacgga ccggccggcg gaccggatgg agcaggccgg
     2701 cgccgcattc ctcgaacggg ttcaggcggc ctaccgggat ctcgcagatg ccgagcccga
     2761 ccggatcctg ctcctcgacg gcgacgcgga tccggacgcg ataacggccc ggatctggtc
     2821 cgacctggag cctcgcctcc cccggtagcg gtcggaaccg cggggcacgg gtccggtaca
     2881 gccggggtac ccgttacgag acgcatggcc ttcgcgaccg ccgaccagct ggaagaggtg
     2941 caccgcctgt tcgcggaatt cctccatgag aggaagcagc ggaggacgcc ggagcgcatg
     3001 gccgttctgg acgcggtcta ctcggccgac ggccacctgg acgccgatgc cctgtacgcc
     3061 cgcctccgcg atggggacgt gcacgtgagc cgtgcgacgg tgtacaacac gctcgagctc
     3121 ctgagcgagt gcgacctggt gacccggcac cagttcggca acaagcaggc gaagtacgag
     3181 cgctcccaca gcttccggca gcacgatcat ctcatctgca tggagtgcgg cgagctgttc
     3241 gaattctgcg atccgcgcct gcagagcgtg caggaaatgg tcgccgaggt gttcggcttc
     3301 gagatcagtc atcactccct tcacatctac gggcactgcc gccggaagga ctgccccacg
     3361 cggcagcccg gggctgacgg atcatgaatt cgtgaaccgt gcagcgcagg ggtttcccgg
     3421 acttgcattg cacgacgggt cgcctatatt tcgctacgcc ctctgcggga tcgtcccggc
     3481 atgaaacgac tcacgctcat accgctcgcc gtgctcctgg cggccgccgc attcccgtac
     3541 ggggtgcgga tcgagtcgtt cgacgtgtcg ccggagggct ccgccttcgt gctccgctgg
     3601 cagaccgata tcgaggacgc ggtggaccat ttcgaggtcc tccgcaagac gcccggcagc
     3661 aacgacgaat tcgtgagggt tcacgaggtc gagtcccatg ggatcggcaa gccgtacgtg
     3721 ttccgggacg accaggtctt caaggtcggg gcggagcagc tcgtctacca gctcgaggcg
     3781 gtcttcgaca acggaacccg gcaggtgctt cacgtccagg cgctgaatta cacgagcacc
     3841 gcggtccgga ggacatgggg cagcatcaag gccatgttcc agtagcccgc ggtcccgcgt
     3901 ccgacgcggg ccgggtcatc aagccgggcg aaccgatggc gcggacgcgg atcatggatg
     3961 cggggcaggt gggtcggacc gtccggcgcc tggccttcga gatcctggag cgcaaccgtg
     4021 gggtggagaa cgtcgtgctc gtgggcatcc gtgagcgggg cgcccggctt tcggaactcc
     4081 tcgccgaggc gctgcagcgg gtcgagaagc agaccgttcc gacctggaca ctcgacgtga
     4141 aggcctatcg cgacgatcgt gatcccgccg tgccgattcc cgcaccctcc gatggccccg
     4201 acctcgcgga ccgcgacgtg gtgctggtcg acgacgtgct cttcaccggg cggacggcgc
     4261 gcgccgcgct cgacgcggtg gtgcggttcg ggaggccgcg ctcgatccag ctggccgtcc
     4321 tggtcgaccg tggacaccgc gagtacccga tccaggcgga cttcgtcggc cgggccattc
     4381 ccacccggca tacggagcgg gtcgtcgtgg acctcggcga cgatccctcg gtggcggtcg
     4441 aggagtagcg ctactgctcc tcttcctccg gcggggcatc gccgggcggc tcgctgaccc
     4501 ggaggcgcac ctcgaatccc tcgcgcacct gcgtacccgg agcggggctc tgggcgagca
     4561 ccaggggctc ctcggcgtcc ggctcgccca gcaccaccga tcgcaggcga agcgacagca
     4621 gccgcgcccg agcgtcgtcc ggcgtgagac cggtcacgtc cgggacctcc acgtatgcct
     4681 cccccagccc ggtgctgtac cacagggtca cgtcggcgcc gtgtggcacg cgggcgcccg
     4741 gcccgggctg ctgccgcgtg accgtgttcg cgtgcgaggc cgggatggaa tccggaagga
     4801 tctcgggcac cgtgaggccg agcaccacga tgcggctctg cgcctcgcgg agggacagcc
     4861 cgatcacccg cgggacggtc acggtggtcg tgtcgcctgt gttgaccgta aggtagatcc
     4921 gtcgaccggg cttcacccgg ctccccgccg gtgggcgctg gtcgatcacc acgtcacgtg
     4981 gcaggttggg cttgcgcaga acgtactcct ccgccgtcag gccggacgcg gcaagcgttc
     5041 cggctgcctc gtcgaacggg agcgtcatca cgtccgggac cacgatcgac acgtcgtagc
     5101 gggtaaacga cggcatgatc gcccggtcca cgacgaggta cgtagcgatc ccgatcaccg
     5161 ccatgacggc gatgccgacc aggaagtagc ggttccggag gatagcgacg agggcgcgca
     5221 ggccgccccg tgcccgggat acgacgcttc ggaccaggcc tcgcccgttc cgggagtctg
     5281 ggtcgatcgg catccgctac ggagattcca tgaatgctgc gggttcggcg gtggcaggga
     5341 aacctgtgtc ggggtcctgt gtttctgcgc tcctgctcca aattagcgcc gaaggctcga
     5401 agggggagtg gaggggattt cggtcgcccc ggggatcgcc cgactctttg aagaggagtt
     5461 caagacgtga caatcgttct tgactctgaa agggtagtcg ccgtatgtta ccgttccgcg
     5521 ccaaattttg gccgggcgcg tttaggcgcc gttctttgat gagactgaat gcatagcgag
     5581 tcctgtcgat tctggccggg tttgacccgg tagagagtcg cacttgtaga gctaacaatg
     5641 tcaaacctct tactatggag agtttgatcc tggctcagga cgaacgctgg cggcgggctt
     5701 aacacatgca agtcgaacga gaagctccag cttgctggag tggaaagtgg cgcacgggtg
     5761 cgtaacgcgt aggcaacctg ccctcggatg gggaataacc acgggaaacc gtggctaata
     5821 ccgcatgacg tccactggtc gcatggccgg tggatcaaag ccttcgggtg tccggggatg
     5881 ggcctgcgtc ggattaggta gttggtgagg taacggctca ccaagcctgc gatccgtagc
     5941 tggtctgaga ggatgatcag ccacactggc actgagacac gggccagact cctacgggag
     6001 gcagcagtga ggaatattgc gcaatgggcg aaagcctgac gcagccacgc cgcgtg
//
LOCUS       DBNHQZ010000260        10889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2110031, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000260 DBNHQZ010000000
VERSION     DBNHQZ010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2110031"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16YU_07015"
     CDS             complement(<1..317)
                     /locus_tag="AC16YU_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065011.1"
                     /translation="MMRALAILAVFLTFVPPRASAQVVVDDFEAYAEGGLPTIWKYQK
                     GGRLHDLSERWMRPKERFTVVAEPACGQVLRAYSEGESVHLALVNGDGALEWDVTEHP
                     VLS"
     gene            complement(317..1276)
                     /locus_tag="AC16YU_07020"
     CDS             complement(317..1276)
                     /locus_tag="AC16YU_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065012.1"
                     /translation="MEEHDQAGWLDRLADRWGAFRDTDAGRRSIRAVRVLFLPVILAV
                     LLWQLLDIGLKELWSNLPTQPLFYILFLVLYFFHILAELVIYRLSWTYRLREALPAFI
                     KKRVYNRDVLGYSGEVYIGVWAQQSLGLARRGVAETVRDNNILSSLASTLVAGVLLAV
                     FLSLGRISIRSLVGDHLLLYAGLAVAVPVVVGLLWLRFRHLIFSMPRNKAVSVFFMQI
                     AHFLVGRAIQILQWVVVLPAVPLGVWFTYAAASILLSRVPFLPNQNLVFAGVGIEMSG
                     MMDVPVAPIAGMLLVNGVLEKLLNILLFTTVSVQDVQPKPAAT"
     gene            1347..2384
                     /locus_tag="AC16YU_07025"
     CDS             1347..2384
                     /locus_tag="AC16YU_07025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6065013.1"
                     /translation="MPTPFLRRLGHRIVYPATLVFSRAGLLAAVYYAFLSSAFWREMK
                     AVLAGRARYLQLVLAGRTNRFLLIRNTHRLEKGLLMRPARDLFALEYIEETVRAFEIA
                     VADGSAGDGGQLGWSNDVLTAYFDAVDGSHPLVRSLAERFRKVALAPGAERRVPYRRR
                     AEAGSQPVAFDDFVALSRHRKSVRWFVDRPVPHDLVRRALAAASLAPSACNRQPYEFL
                     FFDDPGRIATLSTLPMGTKGWSGNIPMFAVIVGDLSAYFSERDRHAIYIDASLAAMSF
                     MLALETLGLSSCPINWPDVGWRERRMSDALGLRPWQRPVLCMAIGYADPEGLVAYSGK
                     RSLDQIARFDG"
     gene            2377..3462
                     /locus_tag="AC16YU_07030"
     CDS             2377..3462
                     /locus_tag="AC16YU_07030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HIR6065014.1"
                     /translation="MADARFVEIRGTEFVNKGAELMMRAILDRLPSGWVPVVEVSRRN
                     TRRQLREVGVRAKRAGVLALPFRPDDVPEDAVSVVLDASGYAYSDFWGPGRARSRLGR
                     HIDRWKARGTAVILLPQAFGPFEGADLRRVMTRALDRADLVFPRDPTSLGHVAGLGLS
                     APVHLAPDFTNLLRSPAPPDADRLRRTVLVVPNRRMIQAGTRGYGDALTASMDRIRSA
                     GYEPALLVQETGRDPGLARRLARRLSPAVPVILESSAVAIKGIIGASAAVVTSRYHSL
                     VCALSQGVPSVATSWSHKYRHLVEEYGYGRSLVPTPESLPAAVSELLDPAVLERDRAQ
                     LLDAAEPVRERARAMWDLVFETIDRRP"
     gene            3485..4615
                     /locus_tag="AC16YU_07035"
     CDS             3485..4615
                     /locus_tag="AC16YU_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6065015.1"
                     /translation="MNILLLTTGLSTVGGRPERFAAGIAGPLHERARIRVVVPRDAGG
                     QDPDTDVPYEVIRVGGGTAGFVAASAAAVRRSVATDGFDVALAMSWHGALAALVGRPR
                     EAPLRIHLVVHGPEMLVSPIPGAGRGTFDRIRAFALGRVDAFHPASLAAARQLAAFGV
                     PESRVHPVELGADPKRFVPVDAFAVRRRLGVGSRPLVLTRAPLEPATGTRTVLQAMAD
                     VLTEHPRTVLVVAGSGSEEPALRGQAAELGIQDAVRFIGTQRPDDLPLLHAAADVFVL
                     VPEPDAAVALAGGSTILEAGSCGVPTVGSRAGDIPDALQDGVTGLLVPPGKPEAVAAA
                     VLFFLKDRGAAAEFGQAARRHVVERANWDRIAERILRTMAPS"
     gene            4714..5139
                     /locus_tag="AC16YU_07040"
     CDS             4714..5139
                     /locus_tag="AC16YU_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6065016.1"
                     /translation="MPHTYESLQIGDSFKIDRMLSPADVAEFAHVTGDDNPLHIDPEY
                     AAGTRFGKPIVHGVLILGVISKVLGRDFPGPGSVAVSIQARFLRPVPVGTTVTYEVRV
                     AEKIEKYRHIRMKVYAYLGGKMVMGGEAVLIPPGEQASA"
     gene            complement(5184..6680)
                     /locus_tag="AC16YU_07045"
     CDS             complement(5184..6680)
                     /locus_tag="AC16YU_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6065017.1"
                     /translation="MKHRTLIPVGLVMALFAATTIVGCAVSKNPLSGRSRAYAYSWQQ
                     EIQLGREADASIVSEYGVYDDPQLTAWVDSLGQALLAQSHLRRPDAEPEWQATEFTFR
                     VLDSPVVNAFALPGGFVYVTRGLVAHAEDEAQLAVVIAHEIGHVAGRHASRRAVTQML
                     GQALLIGGAVGGQAIFGDQVASSILQLGSAATQLLFLSYSRDDERESDQLGVEYAAMA
                     GYDASRGSAFFSTLRRLGEQSGQSLPGFMSTHPDPGEREETILRLAAEWEEKYPMDRV
                     GRDRLLRAVDGIVAGENPRQGFVQGGVFLHPDLAFQFPVPDRFEVVNQASRVVLVGPE
                     QRALVFFGLESEVSTARQAAEAFAATEGLTVIQSGVGSSYGLPAQFVVAEFAAEDGSI
                     TRIRAHYVDYASRVFSFIGLAGQADYASWEPSFLRAMQGFAPLRDPAVLAIQPARIDL
                     TRVSTSTRLSDLIPAEVLGGFTREGIAILNQRPLDALLSSGTTVKLVR"
     gene            complement(6694..7845)
                     /gene="recF"
                     /locus_tag="AC16YU_07050"
     CDS             complement(6694..7845)
                     /gene="recF"
                     /locus_tag="AC16YU_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845230.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR6065018.1"
                     /translation="MHVRSVRLSQFRAHEDSRVSFGSGVTLLYGPNGAGKTNVLEALH
                     YCCLARSFITTSDQYALRRGAEHMEVEAEVSFRTRSDATVRFVLVPREGKRVLVNGSP
                     LERLVDVVGRFPVVAISPDDHRITDGGPDERRRFLDMILCQEKPRYLEALVRYRRVLR
                     QRNEVLARSRSMPGSTLDGLLAPWNEELVRQGSRLTWYRMQFLGRFDPYLDEAFGRIE
                     SVAERPRISYRGFARLDASASEDEIADRFQATLEERGPAERERGVTLVGPHRDELVLR
                     LDDLEVRRYASHGQHRTFVLALRLAQYFYLRDTTGETPLLLMDDVFDNLDPDRIRIFA
                     GLLTSEDAGQAIVTAARGDIFGEAVRFDGDAHRAVRIEEGSPVELSTTS"
     gene            7950..8888
                     /gene="murQ"
                     /locus_tag="AC16YU_07055"
     CDS             7950..8888
                     /gene="murQ"
                     /locus_tag="AC16YU_07055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR6065019.1"
                     /translation="MQQDRELFDELRRLATEQRNPASMAIDTASPEEIVRIINDEDHR
                     VAEAVKGELPYVAEAVRTVVRAFRAGGRLIYVGAGTSGRLGVLDAAECPPTFGSPPEM
                     VQGLIAGGPAAMFVAQEGAEDLEENGARALDEAGVRPSDVVCGIAASRRTPFVVGAVR
                     HARALGCRTLYVTCTPREEFTLPVDVAICPVVGPEVIMGSTRMKSGTAQKLVLNMITT
                     AAMIRLGKVYENMMVDLQMTNQKLVERSRRTIMTIADVDYEAASRLLTDAGGHVKTAT
                     VMHLAGVDAEEARRRLRDASGFVRPAIAGEAYHPVD"
     gene            8956..>10889
                     /locus_tag="AC16YU_07060"
     CDS             8956..>10889
                     /locus_tag="AC16YU_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6065020.1"
                     /translation="MSLRPVQTVLEQSGAFDAVRSWVGSGEPGELGIRRAAGSVPAFL
                     LGDLARRGVALWCILPDSERAAYLHSDLQQVLRDDSSVLLLPPTDHRPYDPDHVDDPA
                     PVIQRADVFQRLADGWRGVLVTGADALLEKVPALRDVTSATLTVSVGPSPGPAPLIAR
                     LSELGFERVEFVEQPGEMALRGGILDVFPFVGDFPVRLEFFGDDIDSIREFDIHTQRS
                     VSRRTSARLVPNLDRDSAADPARRVSLADCLSDDVLVALFDPDSVTAELRTRFERISE
                     AFEARRSTGSDVRPPGDWYLHPEALEAGIAARRRLLFGGVGAPSAEREVDFACRPQPS
                     FNRAMGPLRRQLAVNARNGLRTVILCDSRGQENRLRELLEDEIEPLGVLLTVESLHEG
                     FEAPELGLAVYTDHQIFERYHRPTARRQKQRFGGLSLRELHQLEPGDFVVHVDYGIGR
                     FAGMDRIEVQGRMQEVVRLLYRSDDVLYVNVGALHKLHKYKGKEGHQPELTKLGSGQW
                     ERTKSRTKKRVKDIARDLIQLYARRKRSEGYAFADDSVWQRELEASFAFEDTPDQAAA
                     SDAVKTDMEQPVPMDRLVCGDVGFGKTEIAVRAAFKAVQDGRQVAVLVPTTVLASQHL
                     KTFTRRLDRFPVRVEMLSRFR"
BASE COUNT         1640 a         3953 c         3654 g         1642 t
ORIGIN      
        1 cacgagagca cgggatgctc ggtcacgtcc cattcgagag ccccgtcgcc gttcaccagc
       61 gccaggtgca ccgactcgcc ctccgaatag gcgcgcagca cctgtccgca cgcgggctcg
      121 gcgacgacgg taaaccgctc tttcgggcgc atccagcgct cggacaggtc gtgcagccgg
      181 ccgcccttct ggtacttcca tatcgtcggg agcccacctt ccgcgtaggc ctcgaaatcg
      241 tccacgacca cctgggcgga cgcccggggc gggacgaagg tcaggaagac tgccaggatg
      301 gcgagtgctc gcatcatcag gttgccgctg gtttcggttg cacgtcctgt acggaaacgg
      361 ttgtgaagag cagaatattg agcagcttct ccagcacgcc gttcacgaga agcatgcccg
      421 cgatgggcgc gaccggcacg tccatcatgc cggacatctc gatccccacc ccggcgaaga
      481 ccaggttctg gttcggcagg aagggtaccc gggacagcag gatcgaggcc gccgcgtacg
      541 tgaaccacac gccgagggga accgcgggga gcaccaccac ccactggagt atctggatgg
      601 cccttcctac gaggaaatgg gcgatctgca tgaagaacac ggagaccgct ttgttccgtg
      661 gcatgctgaa gatcaggtgc cggaaccgga gccagagcag gcccacgacc acgggcacgg
      721 ccacggccag ccccgcgtac aggagcaggt ggtcgccgac gagcgaccgg atcgagatcc
      781 gtccgagcga caggaatacg gcgaggagca cgcccgcgac gagcgtcgag gccagcgacg
      841 agaggatgtt gttgtcccgg accgtttccg cgacgccacg cctcgccagg cccaggctct
      901 gctgggccca tacgccgatg tacacctcgc ccgagtatcc caggacgtcc cggttgtaca
      961 cccgcttctt gatgaacgcc ggcagcgcct ccctcaggcg atacgtccac gagagccggt
     1021 agatgaccag ctcggccagg atgtggaaga agtagagaac aaggaacagg atgtagaaca
     1081 gcggctgcgt gggcaggttg gaccacagtt ccttcagccc gatatcgagc agctgccaca
     1141 gcaggacggc caggatgacc ggaagaaaga ggactcgtac ggcccgtata ctgcgccttc
     1201 cggcatccgt gtcgcgaaac gcgccccacc ggtccgccag gcggtccagc cagccagcct
     1261 ggtcgtgctc ttccatggag ccggctccgg atgtggaacg tgaacttcgg tgaatcaggc
     1321 ggcagacggc tgccgtgctc tgctacatgc caacaccctt cctgcgccgt ctcggacacc
     1381 ggatcgtgta cccggcaacg ctggtctttt cccgcgccgg cctgctggcg gcggtctatt
     1441 acgcgttcct ctcctcggcg ttctggcgcg agatgaaggc cgtgctcgcc ggacgggccc
     1501 ggtacctgca gctggtcctc gccggccgga cgaaccgctt cctccttatc cggaacaccc
     1561 atcgcctgga gaaggggctg ctcatgcggc ccgctcgcga cctcttcgcc ctggaataca
     1621 tcgaggagac ggtccgtgcc ttcgagatcg ccgtcgcgga cgggagtgcg ggcgacggcg
     1681 ggcagctcgg ctggtcgaac gacgtgctga cggcctactt cgacgccgtc gacgggtcgc
     1741 acccgctggt ccgctccctc gccgagcggt tccggaaggt cgccctcgct ccgggcgccg
     1801 agcgccgggt tccgtaccgc cgccgcgccg aggccggatc ccagcccgtg gccttcgacg
     1861 acttcgtcgc cctttcgagg caccgcaagt cggtacgctg gttcgtggat cgtccggttc
     1921 cgcacgatct cgtgcgacgg gcgctcgccg cggcctcgct cgcgccgagc gcctgcaacc
     1981 ggcagccgta cgagttcctg ttcttcgacg atcccgggcg gatcgccacg ctgtccaccc
     2041 tgccgatggg cacgaagggg tggtccggga acatccccat gttcgccgtg atcgtgggcg
     2101 acctcagcgc ctatttcagc gagcgggatc gccacgcgat ctacatcgac gcatcgctcg
     2161 cggccatgag cttcatgctg gcgctcgaga cgctcgggct ctccagctgc cccatcaact
     2221 ggccggacgt gggatggcgt gaacgccgga tgagcgatgc gctcgggctc cgtccgtggc
     2281 agcgtccggt cctgtgcatg gcgatcggct atgccgatcc ggaagggctc gtcgcctact
     2341 ccgggaaacg gtccctggac cagatcgccc gtttcgatgg ctgacgcccg cttcgtcgag
     2401 atccggggca cggagttcgt gaacaagggg gccgagctca tgatgcgcgc gattctcgac
     2461 cgtctcccgt ccggctgggt gcccgtcgtc gaggtctccc ggcggaacac ccggcgtcag
     2521 ctccgggagg tgggcgtccg ggcgaagcgg gccggcgtgc tggcccttcc cttccgcccg
     2581 gacgacgtgc cggaagacgc cgtctcggtc gtcctcgacg catcgggcta tgcgtactcg
     2641 gacttctggg gaccgggccg ggcccgcagc cgcctgggcc gtcacatcga ccggtggaag
     2701 gcccgcggaa cggccgtgat tctcctcccg caggccttcg gaccgttcga gggtgccgac
     2761 ctccgacgcg tgatgacccg cgccctcgac cgggccgacc tggtcttccc gcgtgacccc
     2821 acgtccctcg gccacgtggc cggcctcggc ctgtccgcac ccgtgcacct ggcccccgac
     2881 ttcaccaatc tcctgcgctc gcccgccccg ccggacgcgg atcgcctgcg ccgcacggtg
     2941 ctcgtggtcc cgaaccggcg catgatccag gcgggcaccc gcgggtacgg cgatgccctg
     3001 accgcatcga tggatcgcat ccggtccgcc gggtacgagc ccgcccttct cgtccaggaa
     3061 acgggccgcg atcccggcct cgcgcggcgc ctggcgcgcc gcctctcgcc cgccgtgccc
     3121 gtgatcctcg aatcgagcgc cgtggccatc aagggcatca tcggggcctc cgccgccgtg
     3181 gtgaccagcc ggtaccacag cctggtctgc gcgctgtcgc agggggtgcc gagcgtggcg
     3241 acgtcatgga gccacaagta ccggcacctc gtggaggagt acggctacgg acgctcgctc
     3301 gtccctacgc ccgagtccct ccccgcggcg gtgtcggagc tcctcgaccc cgccgtcctt
     3361 gagcgcgacc gcgcacaact cctcgatgcc gcggagccgg ttcgtgagcg ggcccgggcc
     3421 atgtgggacc tggtgttcga aaccatcgac cgtcggccgt gaagcggccc agacccgaac
     3481 tcccttgaac atcctgctcc tgactaccgg gctctccacg gtcggtggcc ggcccgagcg
     3541 cttcgcggcc ggaatcgccg gcccgctgca cgaacgggcg cggatccggg tggtcgtgcc
     3601 gcgcgacgcg ggcggccagg acccggacac ggacgtcccg tacgaggtca tacgcgtcgg
     3661 cggcggaacc gccgggttcg tcgccgcttc cgccgcggcg gtgcggcgca gcgtcgcgac
     3721 ggacggcttc gacgtcgccc tcgccatgtc ctggcacgga gccctcgccg ccctcgtcgg
     3781 gcgcccccgc gaggctccgc tgcgcattca cctggtcgtg cacggccccg agatgctcgt
     3841 gagcccgata cccggcgcag gtcgcgggac tttcgaccgc atccgggcgt tcgcgctcgg
     3901 ccgcgtggat gcgttccacc cggccagtct cgccgcggcc aggcagctcg cggccttcgg
     3961 cgtgccggag tcgcgcgtcc acccggtcga gctcggcgcc gatccgaagc gcttcgtgcc
     4021 cgtggacgcg ttcgccgtcc ggcggcgcct gggcgtcggc agccgacccc tcgtgctcac
     4081 ccgtgctccg ctcgagcccg ccaccggcac ccgaacggtg ctccaggcca tggccgatgt
     4141 cctgacggag cacccgcgca cggtgctggt ggtcgccggg tcggggtcgg aagagcctgc
     4201 cctgaggggg caggcggcgg agctcggcat ccaggatgcg gtccggttca tcggcaccca
     4261 gcgaccggac gacctgccgc tgctccatgc cgccgccgac gtgttcgttc tcgtgcccga
     4321 acccgacgcg gccgtcgcac ttgccggcgg gtcgacgatc ctcgaggccg gctcgtgcgg
     4381 cgtgccgacc gtcggctcac gtgccgggga cattcccgac gcgctccagg acggcgtgac
     4441 gggactcctc gtcccgcccg gcaagcccga agccgtggcg gcggccgtcc tcttcttcct
     4501 gaaggatcgc ggcgcggccg cggagttcgg gcaggccgcc cgccgtcacg tcgtcgagcg
     4561 ggcgaactgg gaccggatcg ccgagcgcat tctccggacc atggccccgt cctgaagcgg
     4621 cccgacccgg gtggccgctg ccgcgcggag gttggcgcgc ccccgcgtga ttcctatact
     4681 cggggccctt cggatccccc aaccgaccgt gccatgccgc acacctacga atccctccag
     4741 atcggagact cgttcaagat cgaccggatg ctttcgcccg ccgacgtggc cgagttcgcc
     4801 cacgtcacgg gagacgacaa tccccttcac atcgaccccg agtacgcggc gggcacccgg
     4861 ttcggcaagc ccatcgtcca cggcgtcctg attctcggag tcatctcgaa ggtgctcggc
     4921 cgcgacttcc ccgggcccgg cagcgtggcc gtctcgatcc aggcccgctt cctccgcccc
     4981 gtccccgtcg gaacgaccgt cacgtacgag gtccgcgtgg cggagaagat cgagaagtac
     5041 cgtcacatcc ggatgaaggt gtacgcgtac ctgggcggca agatggtgat ggggggcgaa
     5101 gcggtgctca tccccccggg cgaacaggca agcgcgtagg ccggcgacgc ctggccgggc
     5161 ggggcgcgcg attcagcccc cgactaccgg accagcttga cggtcgtccc ggaactcaga
     5221 agcgcgtcga gcggccgctg gttcaggatc gcgatgccct cccgcgtgaa tccgccaagt
     5281 acctcggccg gaatgaggtc cgagagcctc gtcgacgtcg agacccgggt caggtcgatg
     5341 cgtgccggct ggatggccag gaccgccggg tcgcgcagcg gcgcgaatcc ctgcatcgcg
     5401 cgcaggaagg acggctccca ggacgcataa tccgcctgcc cggccagccc gatgaaggag
     5461 aacacgcgcg aggcgtagtc cacgtaatgc gcccggatcc gggtgatgct gccgtcttcc
     5521 gcggcgaact ccgccaccac gaactgggcc ggcagcccgt aggacgatcc tacccccgac
     5581 tggatcacgg tcaggccctc ggtggcggcg aaggcctcgg ccgcctgccg ggccgtggac
     5641 acctcggact ccagcccgaa gaagacgagc gccctctgct ccggaccgac gagcaccacg
     5701 cggctcgcct ggttcacgac ctcgaaccgg tccggcacgg gaaactggaa ggccaggtcc
     5761 gggtggagga acacgccgcc ctgcacgaag ccctgcctcg gattctctcc ggccacgatg
     5821 ccgtccacgg cgcgaagcag ccggtcccgg ccgacgcggt ccatcgggta cttctcctcc
     5881 cactcggccg ccagccgcag gatggtctcc tcgcgctcgc ccgggtccgg gtgggtggac
     5941 atgaagccgg gcagactctg cccggactgc tccccgagac gccgcagcgt gctgaagaac
     6001 gccgagcccc gggacgcgtc gtacccggcc atggcggcgt actccacgcc cagttggtcc
     6061 gattcgcgct cgtcgtcgcg gctgtagctc aggaagagaa gctgcgtcgc cgcgcttccg
     6121 agctgcagga tcgaggaggc gacctggtcg ccgaagatgg cctgcccgcc gaccgccccg
     6181 ccgatgagca gggcctgccc gagcatctgc gtgaccgccc gtctggacgc gtgccggccg
     6241 gccacgtgcc cgatctcgtg ggcgatcacc acggccagct gggcctcgtc ctcggcgtgg
     6301 gccacgaggc cgcgcgtcac gtacacgaac cctccgggca gcgcgaacgc gttgacgacg
     6361 ggactgtcca gcacgcggaa ggtgaattcc gtggcctgcc actccggttc ggcatcgggc
     6421 cggcgcaggt ggctctgggc aagcagcgcc tgcccgagcg aatccaccca ggccgtcagc
     6481 tgcggatcgt cgtacactcc gtattcggac acgatagagg cgtcggcctc ccggcccagc
     6541 tggatctcct gctgccacga atacgcgtag gcccgcgagc gcccgctcag cgggtttttc
     6601 gagacggcgc agcccacgat cgtggtcgct gcgaagaggg ccatcaccag gcccaccggg
     6661 atcagggtgc ggtgtttcat ggtcgaccta ttcctatgat gtagtactca actcgaccgg
     6721 gctcccctcc tcgatccgga cggcccggtg ggcgtccccg tcgaagcgga cggcctcgcc
     6781 gaagatgtcc ccccgggcgg ccgtcacgat cgcctgcccc gcatcttccg aagtcagcag
     6841 gccggcgaag atccggatgc ggtccggatc caggttgtcg aaaacgtcgt ccatgagcag
     6901 gagcggcgtc tctcccgtcg tgtcgcgaag gtagaagtac tgggccagcc ggagcgccag
     6961 gacgaacgtc cggtgctgtc cgtgggaggc gtagcggcgg acctcgagat cgtcgaggcg
     7021 caggaccagt tcgtcccggt gaggaccgac gagcgtcact ccccgctccc tttcggccgg
     7081 cccccgctcc tcgagcgtcg cctggaaccg gtccgcgatc tcgtcttcgg aggcggaagc
     7141 gtccaggcgc gcgaagcccc gatacgagat gcgcggccgc tccgccaccg attcgatccg
     7201 gccgaacgcc tcgtccaggt agggatcgaa gcgtcccagg aactgcatcc ggtaccaggt
     7261 gagccggctt ccttgccgga cgagctcctc gttccacggg gcgagcaggc catcgagcgt
     7321 ggatcccggc atcgaccggc tccgggccag gacctcgttc ctctgacgga gcacgcggcg
     7381 gtagcgcacg agcgcctcca ggtaccgggg cttctcctgg cacaggatca tgtccaggaa
     7441 gcggcgacgc tcgtccggcc cgccgtccgt gatccggtga tcgtcgggcg agatggcgac
     7501 gaccgggaac cgtcccacga cgtccaccag gcgctcgagg ggcgacccgt tgacgagcac
     7561 gcgctttccc tcgcgcggga ccagcacgaa gcggacggtc gcatcggagc gcgtgcggaa
     7621 cgagacctcc gcctcgacct ccatgtgctc cgccccccgc cggagcgcgt actggtcgga
     7681 ggtcgtgatg aagctccggg ccaggcagca gtagtgcagg gcctcgagca cgttcgtctt
     7741 gccggcgccg ttcgggccgt aaaggagcgt cactccggaa ccgaagctca cccggctgtc
     7801 ctcgtgggcg cggaactgcg agagtcggac gctgcgtacg tgcatgcacc gatctacgcc
     7861 accggccccg aatcgttcat cctcccgtcg cccgccatat tttgccggcg gccgcgcgcc
     7921 cgccggcctc aaccacccgc aactgagcca tgcagcagga ccgagagctc ttcgacgaac
     7981 tccgccgact ggccaccgag cagcggaatc ccgcctccat ggccatcgac acggccagcc
     8041 ccgaagagat cgtccgcatc atcaacgacg aggatcaccg ggtcgccgag gccgtgaagg
     8101 gcgagctccc gtacgtggcc gaggccgtgc gcaccgtggt gcgggccttc cgggcagggg
     8161 gccgactgat ctacgtcggc gcgggcacga gcgggcgact gggcgtcctc gacgccgcgg
     8221 agtgtccgcc caccttcgga agccccccgg agatggtcca gggcctcatc gcaggcggcc
     8281 ccgccgccat gttcgtcgcg caggagggcg ccgaggatct cgaggagaac ggagcgcggg
     8341 ccctcgacga ggccggcgtc cggccgtccg acgtggtatg cggcatcgcc gccagccggc
     8401 ggacgccctt cgtggtgggc gcggtccggc acgcccgcgc cctgggctgc aggacccttt
     8461 acgtcacctg caccccgcgg gaggagttca cgctcccggt cgacgtcgca atctgccccg
     8521 tggtcgggcc ggaggtcatc atgggatcca cccgcatgaa gagcggcacc gcacagaagc
     8581 tcgtgctgaa catgatcacg acggcggcca tgatccggct cggaaaggtc tacgagaaca
     8641 tgatggtgga cctgcagatg accaaccaga agctggtcga gcgttcccgc cgcaccatca
     8701 tgacgatcgc cgacgtggat tacgaggccg cttcgcgcct gctgaccgac gccggaggac
     8761 acgtgaagac ggctaccgtc atgcacctgg ccggcgtgga tgccgaggag gcccgccggc
     8821 ggctccggga cgcgtccggg ttcgtgcgac cggcgatcgc gggcgaggcg taccacccgg
     8881 tcgactgacg cggcgaccac gcggcgaacc cggcccggga gatttcgttc atccggccca
     8941 agccgtcgcc cttccgtgtc cctccgcccc gtacagaccg tcctcgagca atccggggcg
     9001 ttcgacgccg tccgttcgtg ggtaggctcc ggcgagccgg gcgagctggg catccgccgg
     9061 gcggcgggct cggtgcccgc gttcctgctc ggcgacctgg cccgcagggg cgtcgccctc
     9121 tggtgcattc tgccggattc cgagcgcgca gcctacctgc acagcgacct gcagcaggtt
     9181 ctccgcgacg actcgtcggt cctgctgctt cccccgacgg accaccggcc gtacgacccg
     9241 gaccacgtgg acgacccggc gcccgtcatc cagcgcgcgg acgtcttcca gcgcctggcc
     9301 gacggatggc gcggcgtgct cgtcaccggg gccgacgccc tgctcgagaa ggtgcccgcc
     9361 ctccgggacg tcacgtccgc gacgctgacc gtgtcggtcg gcccttcccc tggccccgcg
     9421 ccgctgatcg cccgcctgtc ggagctcggc ttcgagcgcg tggagttcgt ggagcagccg
     9481 ggggaaatgg cgctccgggg cggcatcctg gacgttttcc cgtttgtcgg cgatttcccc
     9541 gtgcgcctcg agttcttcgg cgacgacatc gactcgatcc gcgagttcga catccatacg
     9601 cagcggtccg tgagccgcag gacgtcggcg cgcctggtcc cgaacctgga ccgcgattcc
     9661 gcggccgatc ccgcccgccg cgtctcgctg gccgactgcc tgtccgacga tgtcttagtg
     9721 gccctcttcg atccggattc cgtcacggcc gagctgcgca cccgtttcga gaggatttcc
     9781 gaggccttcg aggcccgtcg ctcgaccggc tccgacgtgc ggcctcccgg ggactggtac
     9841 ctgcaccccg aggcgctcga agcgggcatc gccgcccgcc gaaggctgct tttcgggggc
     9901 gtcggggccc cgtccgccga gcgcgaggtc gatttcgcct gtcgcccgca gccctcgttc
     9961 aaccgggcca tgggccccct gcggcgccag ctcgcggtca acgcccggaa cggcctgcgc
    10021 acggtgatcc tctgcgacag ccgcggccag gagaaccgcc tgcgcgagct gctcgaggac
    10081 gaaatcgagc ccctcggcgt gctcctcacc gtggagtccc tgcacgaggg attcgaggcg
    10141 cccgagctgg gcctcgccgt ctacaccgac caccagatct tcgagcgcta ccaccgcccg
    10201 acggcccggc gccagaagca gcgcttcggc ggcctgagtc tccgggagct gcaccagctc
    10261 gagcccggcg atttcgtggt gcacgtggac tacggcatcg gccgcttcgc cggcatggac
    10321 cgcatcgagg tccagggacg catgcaggaa gtggtccgcc tcctgtaccg atccgacgac
    10381 gtgctgtacg tcaacgtagg ggcattacat aaactccata aatacaaggg taaagaaggc
    10441 catcagccgg agctcacgaa gctcgggtcc gggcagtggg agcgcaccaa gtcgcgcacc
    10501 aagaagcgcg tcaaggacat cgcgcgcgac ctcatccagc tgtacgcgcg ccgcaagcgg
    10561 tcggagggat acgccttcgc cgacgactcc gtctggcagc gggagctcga agcctcgttc
    10621 gccttcgagg acacgcccga ccaggccgcc gcttccgacg ccgtgaagac ggacatggag
    10681 cagcccgtcc cgatggaccg gctcgtctgc ggcgacgtgg gcttcggaaa gaccgagatt
    10741 gccgtccggg cagccttcaa ggccgtgcag gacggcaggc aggtcgccgt cctggtgccc
    10801 acgaccgtac tcgcatcgca gcacctgaag acgttcaccc ggcggctgga ccgtttcccg
    10861 gtgcgcgtcg agatgctgtc ccgtttccg
//
LOCUS       DBNHQZ010000261         6476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3736892, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000261 DBNHQZ010000000
VERSION     DBNHQZ010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3736892"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="AC16YU_07065"
     CDS             <1..1746
                     /locus_tag="AC16YU_07065"
                     /inference="COORDINATES: protein motif:HMM:NF036968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6065021.1"
                     /translation="SRKPLWHFSPVQVSANGVKVVQFDKDDVEALGLVKFDVLGLRML
                     ACLSEAVEIAERHDCFVPGAPGVTGDGAFDVDELPLDDAATYNLMRSSKTLGVFQIES
                     QGQLHLLAQHQPEDFIDIINEIALFRPGPLQSGMVHPFVQRRRGLKPVEYPHPDLEPI
                     LGETYGVIVFQEQVLEVAHHFARMSLEEADEFRRLMSKFRDPGQMEGMRERFVSGAVA
                     HGVDPTTATAVFENVSKFVGYGFCRSHAAAFAKTVYQSAYMKTHYPAAFMAAFLQHRP
                     GMYNLMTLEEECRRFGVEILLPDVNRSGARYDLERLPDGRLAIRKPLTAIQGMSEEHA
                     REIVWERLNGPYRTVQDFYERVALDADLFDGVARSGALDALAGTSRRALWEMGLLERR
                     LAAPGSRAALLFEAPAFSEDDVPELPTLTAEERFAWDYQVHGAARAHPMTLVRRTLSD
                     YEIRPIATCWGLGRAVRETRDGPVVTIAGIAMLRQRPPTANGVLFLTLEDETGFIQCF
                     VYPKVLEALDHVVSQSSLIVRGKLQVMGNWRGLVMHHAWPLNGILGGYEGHPSMAGGR
                     DRMVTGVEVRVVGAA"
     gene            complement(1840..2928)
                     /locus_tag="AC16YU_07070"
     CDS             complement(1840..2928)
                     /locus_tag="AC16YU_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR6065022.1"
                     /translation="MIRRLLLALALLLPACSLDPDDGDPGSGRLIVANGGNFADQNGF
                     LSRIDLETGAVADGPSLDGFLQGLHPAGDEVAILVNTFGDGRVDFAGTRDLVPVRSVT
                     GIDSPRGAASLGERLFVTTFAADGTGAVLEIDAASASVLKSWPAGGPFPEGIAVAAGR
                     LFAARYGFLGDGTSLAVIDPGADGVDTLELGCDGPRDVLALGGADLLVVCQGKTVYSP
                     DWTEILEMTPGQVVFVDGAAGAVSGRVELDGQIAGSNQAQSSVFVTGLGAAFLLDGPG
                     ARILEVDVSKRSASWLSPVWSDSGVGLSGVAWDPVGSRLFVGRLARGAGGVPDFAAAG
                     VVTAVTPSGDPASSYRVGPSPSGIVLLP"
     gene            complement(2925..4796)
                     /locus_tag="AC16YU_07075"
     CDS             complement(2925..4796)
                     /locus_tag="AC16YU_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6065023.1"
                     /translation="MTFAFRPAAFLLALAPLAAGAAAQQRADSVHVLGPILVEAERPS
                     TAPRGVRSIRVDRDGLAPDDLVTVLEREAGLYALRTGTGAAPAFRVRGADDLQTAVLL
                     DGIALRDPHTGSFDPGLVPRVVLESAEIGLGAAGSDAGAPTGPGLGGAIRLSTGVAGG
                     CGGVRVEAAAGAYGALRAGGRIASGSDRLCLLLAAEHGEEAGDFPVPAIPGAPDRRLG
                     AERSHESGFVRLSGAGGPLRWSITGWGSASGAGLPGPANSMPRGAEMDTDSWRGAASV
                     RRLGSRIDVGLSLQAGHRLGTFRDPAADRLDRAEATTVQGELSLAATLSRAWRGESRL
                     VLGLDRSMLPVADRQRRSVMDVSAHRSGDAGEIALGLAAGRWPGAGVLVAPRLSAGTR
                     GRWSLRASVARAVRPPTLAERAWVPGGNPDLRPETGWSADAGVSFESGTGWTIDLGAY
                     VNHLRNRIVWQPRLVAPGLDVWTPSNVGRSLGLGVEPWVERTFRAGSGRVRMRLAGTI
                     GRTTDRSDPLAASYGHPLRHLPGSVWSALAEWASDRVALRLDGRRVGRRPITSDDSRH
                     LPAFTVLDVSGSLAVGRPLRLRASLRNALGARYAMIPNYPMPGRHLTLTLTLDLPRP"
     regulatory      complement(4886..5139)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5245..5748)
                     /locus_tag="AC16YU_07080"
     CDS             complement(5245..5748)
                     /locus_tag="AC16YU_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6065024.1"
                     /translation="MNKSTNRSRIADVTRWLDPQKGGLWHGGPSPLSAVRGVSAEAAA
                     WKPAPDRHSIWELVLHVAYWKYAVRRRLSGDPPGGFPRSPANWPALPGNRDEAAWKRD
                     RALLRDTHRALLEVIGGLDPARLDDLDGRGGRWTLMDLLSGIVLHDTYHSGQIQLMKR
                     LHRSMTT"
     gene            5875..>6476
                     /locus_tag="AC16YU_07085"
     CDS             5875..>6476
                     /locus_tag="AC16YU_07085"
                     /inference="COORDINATES: protein motif:HMM:NF020653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6065025.1"
                     /translation="MHAPRYRTLWLPVVLALLRAAPATAQQIDRIEPPNWWVGMRTNA
                     VQLMVHGEDLHGATARFDEPRLEVTAVRTLESGSYLFVDVVVPEDLEPGAYSLTVWRG
                     GWEASLDFPLLARETDPARHRGFSSDDVVYLVTPDRFANGDPANDCVAGLDECADRSR
                     AGARHGGDLEGLIRRLDYLADLGITALWLNPVLENAGRAS"
BASE COUNT          947 a         2317 c         2359 g          853 t
ORIGIN      
        1 tcccgcaaac cactctggca cttctccccg gtgcaggtgt cggccaacgg ggtgaaggtg
       61 gtacagttcg acaaggacga cgtggaggcc ctcgggctcg tcaagttcga cgtgctgggt
      121 ctgcggatgc tcgcgtgcct gtccgaggcg gtcgaaatcg cggagcggca cgactgcttc
      181 gtaccgggcg cgccgggggt cacgggcgac ggggcgttcg acgtcgacga gctcccgctc
      241 gacgacgcgg ccacctacaa cctgatgcgc tcgtcgaaga cgctcggcgt cttccagatc
      301 gagtcgcagg ggcagctgca cctcctggcg cagcaccagc ccgaggactt catcgacatc
      361 atcaacgaga tcgcgctctt ccggccgggc ccgctgcagt cgggcatggt gcacccgttc
      421 gtgcagcgga ggcggggcct gaagccggtc gagtacccgc atcccgacct ggagccgatc
      481 ctcggcgaga cgtacggcgt catcgtgttc caggagcagg tgctcgaagt cgcgcaccac
      541 ttcgcccgca tgtcgctcga ggaggcggac gagttccgcc gcctcatgtc gaagttccgc
      601 gatccgggcc agatggaggg gatgcgcgag cggttcgtct cgggtgcggt cgcgcacggg
      661 gtggacccca cgaccgcgac cgcggtcttc gagaacgtgt ccaagttcgt gggctacggt
      721 ttctgccgca gccacgcggc cgccttcgcg aagacggtct accagtcggc ctacatgaag
      781 acgcactacc cggcggcttt catggcggcc ttcctgcagc accggcccgg catgtacaac
      841 ctgatgacgc tcgaggagga gtgccggcgc ttcggggtgg agatcctgct gccggacgtg
      901 aaccgctcgg gcgcccgcta cgacctggag cgcctgccgg acggcaggct cgccatccgc
      961 aagccgctca ccgcgatcca ggggatgtcg gaggaacatg cccgtgagat cgtctgggag
     1021 cgcctcaacg ggccctaccg gacggtgcag gacttctacg agcgggtcgc gctcgacgcg
     1081 gacctcttcg acggcgtcgc ccgctccggg gcgctcgacg cgctcgccgg cacgtcgcgc
     1141 cgcgcgctct gggagatggg cctcctggag cgccgcctgg ccgctcccgg gtcgcgcgcc
     1201 gcgctcctct tcgaggcgcc cgccttctcg gaggacgacg tgcccgagct cccgacgctt
     1261 accgcggagg aacgcttcgc ctgggactac caggtgcacg gggctgcccg ggcccacccg
     1321 atgaccctgg tccgccgcac gctgagcgac tacgagatcc ggcccatcgc cacctgctgg
     1381 ggcctggggc gcgccgtgcg ggagacgcgc gacgggcccg tcgtcacgat cgccggcatc
     1441 gccatgctgc ggcagcggcc gccgaccgcg aacggcgtcc ttttcctcac gctcgaggac
     1501 gagacgggct tcatccagtg cttcgtgtac ccgaaggtgc tcgaggcgct cgaccacgtg
     1561 gtctcgcagt cgagcctcat cgtgcgcgga aagctgcagg tgatgggcaa ttggcgcggt
     1621 ctcgtgatgc accacgcgtg gccgctcaac ggcatcctgg gcggctacga gggtcacccg
     1681 tcgatggcgg gcggacggga ccggatggtc acgggggtgg aggtacgggt tgtgggggcg
     1741 gcgtgaatcc tgctccgccc gtccgacggc ctcggacgtt ccgtacatcg aatcgctcgc
     1801 gtacgcgaag agccagccgc ggtgtcgtgg actccgcgcc taaggcagca ggacgatgcc
     1861 ggagggggaa ggccccacgc ggtagctgct cgccggatcg cccgacggcg tgacggccgt
     1921 cacgacgccc gccgccgcga agtcgggaac gcccccggcc cccctcgcca ggcgcccgac
     1981 gaacaggcgg gagcccacgg gatcccaggc gacgccggag aggccgacgc cggaatcgga
     2041 ccacaccggg gagagccagg aagcgctccg cttcgatacg tccacctcca ggatgcgcgc
     2101 tcccggccca tcgagcagga acgcggcgcc gagcccggtc acgaagacgg acgattgcgc
     2161 ctggttcgag cccgcgatct gcccgtccag ctccacgcgg ccgcttaccg cacccgcggc
     2221 gccgtccacg aagaccacct gcccgggcgt catttccagg atctcggtcc agtccgggct
     2281 gtagacggtc ttcccctggc atacgacgag gaggtcggcc ccgccgagag cgaggacgtc
     2341 gcgcgggccg tcgcacccga gctcgagcgt gtcgacgccg tccgcaccag ggtcgatgac
     2401 ggcgagcgag gtgccgtcgc cgaggaagcc gtaccgcgcg gcgaacagcc gcccggcggc
     2461 aaccgcgatg ccctcgggga acgggccgcc cgcaggccag gacttcagga cggaggcgga
     2521 cgccgcgtcg atctcgagca cggcgccggt cccgtcggcg gcgaatgtcg tcacgaacag
     2581 ccgctcgccg agcgacgccg ccccccgcgg cgagtcgatg ccggtcaccg atcgcacggg
     2641 gaccaggtcg cgtgtcccgg cgaagtccac gcgcccgtct ccgaacgtgt tgacgaggat
     2701 cgcgacctcg tcgccggccg ggtggagacc ctgcaggaat ccgtcgagcg acggtccgtc
     2761 cgcgaccgcg cccgtctcga ggtcgatgcg cgacaggaag ccgttctgat cggcgaagtt
     2821 gcccccgttg gccacgatca ggcggcccga gcccggatcg ccgtcgtccg ggtccaggga
     2881 gcaggcaggg aggaggagcg cgagggcgag aaggaggcgc cggatcatgg acggggcagg
     2941 tcgagcgtga gggtgagcgt gagatgccgg ccgggcatcg ggtagttggg gatcatggcg
     3001 tagcgggcgc cgagggcgtt ccggagcgag gcccggagcc gtagcggacg cccgacggca
     3061 agcgagccgg agacgtcgag gacggtgaag gccgggagat gccgcgagtc gtcggacgtg
     3121 atcgggcggc ggccgacgcg ccggccgtcg agcctcagcg cgacccggtc cgacgcccac
     3181 tccgcgagtg cggaccagac ggagccgggc aggtggcgca gcggatggcc gtacgaggcg
     3241 gcgagcggat ccgagcggtc ggtggtgcgg ccgatcgttc cggcgaggcg catccgcacg
     3301 cgaccgctcc cggcgcggaa cgtgcgctcc acccacggct cgacgcccag gcccaggctt
     3361 cggcccacgt tcgacggcgt ccagacgtcg aggcccggcg ccacgaggcg aggctgccac
     3421 acgatccggt tccgcaggtg gttcacgtac gcacccaggt cgatcgtcca gcccgttcca
     3481 ctctcgaagc tcacgccggc gtccgcgctc cagccggtct cggggcgcag gtcggggttg
     3541 ccgcccggaa cccaggcgcg ctccgcgagc gtggggggac ggaccgcgcg cgcgaccgag
     3601 gcccggagcg accagcgccc ccgtgtcccg gccgagaggc gaggggcgac caggacgccc
     3661 gccccgggcc accggccggc cgcgaggccg agcgcgattt cgcccgcatc acccgagcgg
     3721 tgcgccgaga cgtccatgac cgatcgacgc tgccggtcgg cgacgggcag catgctccgg
     3781 tccaggccga gtaccaggcg ggattccccg cgccaggcac gggagagggt ggctgcaagc
     3841 gagagctcgc cctgcacggt cgtcgcctcg gcccgatcca ggcggtccgc ggccggatcg
     3901 cggaaggtgc cgagtctgtg cccggcctgc agactgagcc ctacgtcgat ccgcgagccg
     3961 agacggcgga cggaggctgc gccccgccac gaatccgtgt ccatttcggc tccgcgcggc
     4021 atgctgttgg ccggtccggg aagtccggcg ccggacgccg agccccagcc cgtgatggac
     4081 caccgcagcg ggccaccggc gcccgacagc cggacgaacc cggattcgtg cgagcgctcg
     4141 gcccccaggc ggcgatccgg cgcgccggga atcgcgggaa cggggaagtc gccggcctcc
     4201 tcgccgtgct cggcggccag gagcaggcac agccggtccg atcccgaagc gatgcgccct
     4261 ccggcacgca gcgcaccgta ggccccggcc gccgcctcta cccgcacgcc tccgcagccg
     4321 ccggccacgc ccgtcgacag gcggatggct cccccgaggc cggggccggt cggagcgccc
     4381 gcgtccgaac cggcggcccc gagcccgatt tcggccgatt ccaggaccac gcgcgggacc
     4441 aggccgggat cgaagcttcc agtatggggg tcgcgcagcg cgattccgtc cagtaagacg
     4501 gccgtctgca ggtcgtccgc gccccggacc ctgaatgcgg gcgccgcacc cgtgccggtt
     4561 ctcagggcat agagtccggc ctcccgctcc aggaccgtga ccaggtcgtc cggcgccagc
     4621 ccgtcccggt ccacgcggat cgaacggacg ccccggggcg ctgtcgaggg ccgctcggcc
     4681 tcgaccagga ttggtccgag gacgtgcacg gagtccgcgc gctgctgcgc ggcggcgccc
     4741 gcagcgagcg gtgcgagcgc gaggaggaat gcggccggcc gaaaggcgaa ggtcatcgtg
     4801 ggcctcgact ccaagggagc cgggaccacg ggaggcggcc gggggcgcgc gctcggcgat
     4861 accggactcc acgacccggg agtcttcggc aggatgcacg ccggcaggtc tcctggcttc
     4921 cgctcgcacc ggaacgggtc cggttcaccg gcaggctccc cgtccggcct tcccgcgccg
     4981 gtgcgcagtg gtcggacggc tcacggccgt cggtgttcga gcggtcacag ttgcgggtac
     5041 agccccggaa tccacgcgcc gatcgggcgg cgtgtcaccg ggttccctct tggccgcgac
     5101 cggcgatcgg ccgcggaacc gcgcgtgcac atgggaaaga acgcccgaac ttacggcagg
     5161 ggcgatccag cttcaatcgc cgactgcccc ccgcccggga cgcactcgcc ccgcgactcc
     5221 gggggcgccc gcggatgacg tccgtcaggt cgtcatcgag cggtgaagcc gcttcatgag
     5281 ctggatctgc cccgaatggt aggtatcgtg gagcacgatg cccgacagca ggtccatcag
     5341 ggtccatcgc ccgccccttc cgtcgagatc gtccaggcgc gcggggtcga gtcccccgat
     5401 gacctccagg agggcgcggt gcgtgtcgcg gagcagcgcc cgatcccgtt tccacgccgc
     5461 ctcgtcccga ttcccgggca gcgcgggcca gttggcgggg ctccggggaa agccccccgg
     5521 ggggtctccg gagaggcggc gacgtacggc gtacttccag taggcgacgt gcaggacgag
     5581 ctcccagatg gagtgccggt ccggcgccgg cttccaggcc gcggcctccg cggacacgcc
     5641 gcgaacggcc gaaagaggcg agggaccgcc gtgccagagt ccgcccttct gcgggtccag
     5701 ccagcgggtg acgtcggcga tacgggaccg gttcgtggac ttgttcatgg catggcggtg
     5761 gaaagatcga gctgaccgca tcgttacgct ccgggtgccg ggagagattc tgtcgcaccg
     5821 cgacgcgcgg accgcgatat tcgggcatta cgacgtccca cctcgatgtg cgccatgcat
     5881 gcccccaggt accggaccct gtggcttccg gtcgttcttg cgcttcttcg cgccgccccg
     5941 gccacggccc agcagatcga ccgcatcgag ccgcccaact ggtgggtggg aatgaggacg
     6001 aacgccgtgc agctaatggt ccatggcgag gacctccacg gcgcgacggc ccgattcgac
     6061 gagccgcgcc tcgaggtgac ggccgtgcgc acgctcgaga gcgggtcgta cctgttcgtg
     6121 gacgtggtcg tgccggagga tctggagccc ggcgcgtact cgctgaccgt ttggcgcggc
     6181 gggtgggagg cctctctgga cttcccgctc ctcgcgcgcg agaccgatcc cgcgcggcac
     6241 cggggcttct cgagcgacga cgtggtctac ctcgtcaccc cggaccgctt tgccaacggc
     6301 gacccggcga acgactgcgt cgccgggctc gacgaatgcg ccgaccgctc ccgagccggc
     6361 gcccggcacg gcggcgacct cgagggcctc atccggcgcc tcgactacct ggcggacctc
     6421 ggtatcacgg cgctgtggct caacccggtg ctcgagaacg cggggcgcgc gtcgta
//
LOCUS       DBNHQZ010000262         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1028967, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000262 DBNHQZ010000000
VERSION     DBNHQZ010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1028967"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="AC16YU_07090"
     CDS             complement(<1..879)
                     /locus_tag="AC16YU_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6065026.1"
                     /translation="MTSDLAIAALADAALAWRDPDFPRRAEAVRRTLGEGNRFTGEAL
                     AFALNQAMAEIDRSTLRRWQGGRAAERPLRVGVLEAGNVPLAGLQDYLAVQVVGHAFV
                     GATSSRSPHLLPAFAAEVVERGGPSAEFVPRDALFARAEAVVASGSDETRDAVAALCD
                     AAGIPVGRRLLRGTRFGVAVLDGRESEDERLALAEDALLHEGEGCRNVAIVWAPEDLA
                     PDDVLEAFAVFRGTFPVHPSSAGSLRMQQAFLKAVDAPHAHADGMAFLVSRGAPEVQP
                     PGHVRWVPYSDIGQAAA"
     gene            complement(869..1213)
                     /locus_tag="AC16YU_07095"
     CDS             complement(869..1213)
                     /locus_tag="AC16YU_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HIR6065027.1"
                     /translation="MHLPEDGVLLRVFVGESDTWEGKSLYRAIVEEARRMNLAGATVL
                     RGIMGFGAASRIHTTRFLRLSEDLPIVIEIVDTRERVEEILPFLDRVVKGGLVTMEKA
                     RVIRYRHAGYDE"
     gene            complement(1216..1596)
                     /gene="crcB"
                     /locus_tag="AC16YU_07100"
     CDS             complement(1216..1596)
                     /gene="crcB"
                     /locus_tag="AC16YU_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR6065028.1"
                     /translation="MAGRLLVIALGGALGAVLRYGLSGLAYRLAGEGFPWGTLAVNLS
                     GCLAIGILWAAFERAPVPAEWAAFLFIGLLGAFTTYSTFALESMNLLRDGETILALAN
                     ILASTVGGILLTFAGLSATRSILG"
     gene            complement(1596..2786)
                     /locus_tag="AC16YU_07105"
     CDS             complement(1596..2786)
                     /locus_tag="AC16YU_07105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6065029.1"
                     /translation="MEDVFVVSAARTPIGRFGGALKDHSPVDLAAHVMKAAVERSGVE
                     AGSLDLVLFGNILRAGYGQLIPRQASLRAGIPDHVDSVAIDMVCSSGMMAVMQAATQI
                     RAGESDLLLAGGVESMSGAGFYLSSRARWGYKFLMGAPEGLKDILLEDGLMDPTTGEP
                     MGEQAERTAAEHGVTRQQVDEVAAASHRRAAEAWEKGWFTAESTPIGYRVKRDTVMLE
                     RDEGIRADTTLESLASLRPAFSKEGVLTAGNASQISDGAAALVLASGSAVRAHGLKPL
                     ARLVGGAWSAGETWRFSEAPIPAVRKLLDRTGEGVADFDLFENNEAFALSSVLFSTML
                     GVPPDRLNVHGGAVALGHPIGCSGARIVVTLVHALATHGHSRGLAAICHGTGGSTAVT
                     VERA"
BASE COUNT          461 a         1094 c         1000 g          395 t
ORIGIN      
        1 ggcggccgcc tgcccgatgt ccgaataggg gacccagcgc acgtgccccg gcggctgcac
       61 ctcgggcgcg ccccggctca cgaggaaggc cattccgtcc gcgtgggcat gcggcgcgtc
      121 caccgctttc aggaaggcct gctgcatccg gagacttccc gcactcgaag ggtgcaccgg
      181 gaacgtcccg cggaagacgg cgaacgcctc gaggacatcg tccggagcga ggtcttccgg
      241 ggcccacacg atggccacgt tccggcagcc ctccccctcg tgcagcagcg cgtcctcggc
      301 gagcgcaagc cgctcgtcct ccgactcgcg cccgtcgagt accgcgacac cgaacctggt
      361 gccccggagc aggcgtcgac cgacagggat tccggccgcg tcgcagaggg ccgcgaccgc
      421 gtcgcgtgtc tcgtccgatc cgctggctac gacggcctcg gcccgggcga aaagcgcatc
      481 gcgcggcacg aactccgctg agggcccgcc acgctcgacc acttccgcgg caaaggctgg
      541 aaggaggtgc ggcgaccgcg acgacgtggc cccgacgaac gcgtggccga cgacctggac
      601 cgcgaggtaa tcctggagac cggcgagggg aacgttgccg gcctcgagca cgcccacgcg
      661 gagtggccgc tccgcggctc gtccgccctg ccagcggcgg agcgtcgacc gatcgatctc
      721 ggccatcgcc tggttcagcg cgaaggccag ggcctcgccc gtgaaccggt tgccctcgcc
      781 gagcgtgcgc cggaccgcct cggcccgccg cgggaaatcg ggatccctcc aggccagggc
      841 ggcgtcggcg agagccgcga tcgcaaggtc actcgtcata gcccgcgtgt cggtaacgga
      901 tgacccgcgc cttctccatg gtcacgagcc cgcccttcac gacgcggtcc aggaatggga
      961 ggatctcctc gacccgctcc cgggtgtcca cgatctcgat gacgatcggc aggtcttcgg
     1021 acaggcgcag gaagcgcgtc gtgtggatgc ggctggccgc gccgaatccc atgatgcccc
     1081 ggagcaccgt ggccccggcg aggttcatcc gtcgcgcctc ctccacgatg gcccggtaca
     1141 gggacttccc ttcccacgtg tcggattccc cgacgaagac gcgaagcagg acaccgtctt
     1201 cgggcagatg catggctatc ccaggatgga gcgggtcgcg gacagcccgg caaacgtgag
     1261 caggatccca cccacggtcg aggccaggat gttcgcgagc gcgaggatcg tttcgccgtc
     1321 ccgcagcagg ttcatggact ccagggcgaa cgtggagtac gtggtgaagg ctccgagcag
     1381 cccgatgaac aggaacgcgg cccactccgc aggcaccggc gcccgctcga acgcggccca
     1441 gaggatgccg atcgccaggc atcccgagag gttcaccgcc agcgtgcccc aggggaagcc
     1501 ctccccggcc agtcggtagg ccagcccgga aaggccgtac cggagcacgg ctccgagcgc
     1561 cccaccgagg gcgatgacca ggagccggcc cgccatcagg cccgctcgac ggtaacggcg
     1621 gtgctgccgc cggtcccgtg gcagatcgcc gccagtccgc gcgagtgtcc gtgcgtcgcg
     1681 agcgcgtgca cgagcgtgac cacgatgcgg gctccggagc agccgatggg atgcccgagc
     1741 gcgacggcgc cgccgtgtac gttcagccgg tcggggggca cgcccagcat cgtcgagaag
     1801 agcacgctcg agagcgcgaa cgcctcgttg ttctcgaaaa ggtcgaagtc ggccacgccc
     1861 tcgcccgtcc ggtccaggag cttccgcacg gccgggatcg gggcctcgga gaagcgccac
     1921 gtctcgccgg ccgaccaggc cccgcccacc aggcgcgcga gcggcttcag gccgtgcgcc
     1981 cggaccgcgc tgccgctcgc gagcacgagt gccgcggccc cgtccgagat ctggctggcg
     2041 ttcccggccg tgagcacgcc ttccttcgaa aaggccggcc gcagcgaggc gagcgactcc
     2101 agggtcgtgt ccgcgcggat gccctcgtcg cgctccagca tgaccgtgtc gcgcttcacg
     2161 cggtagccga tgggcgtcga ctcggccgtg aaccatccct tctcccaggc ctccgcggcc
     2221 cgccggtggg atgccgccgc cacctcgtcg acctgctgcc tcgtcacccc gtgttccgcc
     2281 gccgtccgct cggcctgctc gcccatcggc tccccggtcg tcggatccat caggccgtcc
     2341 tcgagcagga tgtccttcag gccctccgga gcacccatca ggaacttgta cccccaccgg
     2401 gcccgcgacg agaggtagaa gcccgccccg gacatagact cgacgccgcc ggcaagcagg
     2461 aggtccgact cgcccgcccg gatctgcgtc gccgcctgca tcacggccat catgcccgac
     2521 gagcacacca tgtcgatcgc caccgagtcc acgtgatccg ggatcccggc ccggagcgac
     2581 gcctggcgcg ggatcagctg gccgtacccg gcgcgcagga tgtttccgaa gagcaccagg
     2641 tccagggatc ctgcctccac gccggagcgc tcgaccgcgg ccttcatcac gtgggcggcc
     2701 aggtcgaccg gcgaatggtc cttcagggct ccgccgaagc gcccgatggg cgttcgcgcg
     2761 gcggaaacga cgaagacgtc ttccatgaaa tcctcgggct tgaatcgatg ggaggcggaa
     2821 gctacgagcc tcgtcgggtg aaggtcgtcc ggaagcgctt ttttatcccg ccttcatgcc
     2881 ggccgggggg cgctccggac gcttaagcaa gtccgcgccc ggacgatacc ggaaccagcg
     2941 gtctcccagg
//
LOCUS       DBNHQZ010000263         4333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_350201, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000263 DBNHQZ010000000
VERSION     DBNHQZ010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_350201"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC16YU_07110"
     CDS             complement(<1..183)
                     /locus_tag="AC16YU_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065030.1"
                     /translation="MLLEFIIVHSSAFMGSVILSREAPARRLRNVLGLGLFYTLFVGG
                     FAAGFGTWWPLWAFWGL"
     gene            644..1912
                     /locus_tag="AC16YU_07115"
     CDS             644..1912
                     /locus_tag="AC16YU_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6065031.1"
                     /translation="MDALENVDVAVVGAGQAGLVVSYLLSEARVEHVVLERGRIGESW
                     RSQRWDTFFLNTPNWSSGLVGMEFRPEGPDGFSTSEDLTGYFEEYARVFELPIRERTE
                     VRALGSRPSGGYVLSAGSTEFHADAVVIASGSMSRPRVPAMAANLPDDIACLSAGTYH
                     NPEALPDGAVVVVGSGQSGCQIAEDLLEAGREVFVCASRVGRIPRTYRGQDIFHWLRH
                     MNFFEARPEDLDDQAMRYAAQPQVSGRYGGHTVSLQSLARDGATLLGRVEGVDGHVLS
                     LGSNLRECVAFGDEISRMIKLGIDGYIERAGIQAEPPTPDPGEPALPDLKGSDSWRSF
                     DLRANGVSAVVWCTGFDADWSWVNLDVFDDRGEFRHDDGITDSPGLYVCGFPWVSKRK
                     SGLLYGVAEDAERIVRHIESYLGAKAGRPS"
     gene            1909..2337
                     /locus_tag="AC16YU_07120"
     CDS             1909..2337
                     /locus_tag="AC16YU_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6065032.1"
                     /translation="MSVRAGWSTPLLNVESIERSIPFYRELGFETIDVEGEAGCLGWA
                     RLHCEGGAIMLLRSDEPSPSTEDRFLLYLYTPDLPALRARLVAASVEVTRIQHPEHMR
                     SGEMSFRDPDGYTILVGHWGETEHEAWLRRIEEKRAAGLI"
     gene            complement(2365..3180)
                     /gene="arsM"
                     /locus_tag="AC16YU_07125"
     CDS             complement(2365..3180)
                     /gene="arsM"
                     /locus_tag="AC16YU_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR6065033.1"
                     /translation="MNTQATPTPTELKDIVRGKYTEIVQTQSSCCGSSCCGSETVPVD
                     LSDDYTTLEGYMPEADLGLGCGLPTEHAGIRPGDTVLDLGSGAGNDAFVARSLVGEKG
                     RVVGVDMTPAMIQRARANADLIGARNVEFRLGDIEALPVDDAEVDVIVSNCVLNLVPN
                     KAMAFEEMFRALRPGGHFCVSDIVSRGELPASVRGVAELYAGCVAGAIDREAYVRMLE
                     DAGFADVRVVEEKIVDVPDDALEGVVDLAEVRAYRESGGALLSVTVVGWKPGG"
     gene            complement(3217..3573)
                     /locus_tag="AC16YU_07130"
     CDS             complement(3217..3573)
                     /locus_tag="AC16YU_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6065034.1"
                     /translation="MSPSTTIARITQAGACCAPRDVPLPPDETLDEVSRALKALSHPV
                     RLRIMHILGGAGGSVCACDVEARFDLSQPTISHHLRLLREAGLVETEHRGPWVHYSPR
                     RSAIGALARQVAGLTG"
     gene            complement(3717..>4333)
                     /locus_tag="AC16YU_07135"
     CDS             complement(3717..>4333)
                     /locus_tag="AC16YU_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR6065035.1"
                     /translation="FVDNGLGSWILNSADAHLYNNTYVDSPVRFSRDGRSAEGDHFNW
                     HPATGPGVTEREGHVFLKNLVVTSRPGVTLLGVGQPPYLCDQLTEPALAAMDGNVYVR
                     PASPYESLAGPIVTWVDADAAGCTAGFESLEAFKEQVPGFEAHGQELEGDARSVFIAP
                     DLGRYELRRALPASREVAMPPDVREWLGWSAEEAEGAVGAYGGE"
BASE COUNT          698 a         1457 c         1496 g          682 t
ORIGIN      
        1 caggccccag aaggcccaga gcggccacca ggtgccgaac ccggccgcga agccgcccac
       61 gaagagggtg tagaagagcc ccagccccag gacgttgcgc agccttcgcg ccggcgcctc
      121 gcgcgagaga atcacgctgc ccatgaatgc cgacgagtgc acgatgatga attcgagcag
      181 catcgtcagc aggtagtacg gtacggcctt cgcgccgagg gcgaaggggt cgatccacgt
      241 gacgaggaac gccccggcca gcgcgaagtc gggcagcgcc gcgaggacgc cgcgggcggt
      301 gacgccggcc gaggccgatg cgtcggcgtg ggcggtcgat atgatgttcg tcgggggcag
      361 gggctcgcgg cgcggcgccg ggcgcgctcg gtgtccccga acctatcggc cggcgggggc
      421 gggaggcaag cggccggccc cgtgtgggct tccgatcgtc cgtgcggtta ccccgggctc
      481 gaaccgcgcg catgcactcc gaacagaacc ggaaccacgt gccggccgag ctcaagcggt
      541 tcgtggagga gtgatcggca cgcgcgcgcc ggacatcgaa cctggcattt cttatcgtgt
      601 cccctcgaag tggccgctac cccacacgag tcccgtcaga gaaatggatg ctctcgagaa
      661 cgtcgacgtc gccgtggtcg gcgccggtca ggcaggtctg gtcgtcagct acctgctgtc
      721 cgaggcccgg gtcgaacacg tcgttctgga acgcggaagg atcggcgagt cgtggcgctc
      781 gcagcgctgg gacacgttct tcctgaacac gcccaactgg tcgagcgggc tcgtggggat
      841 ggagttccgc ccggagggtc ccgacggatt cagcaccagc gaggacctta cgggctactt
      901 cgaggagtac gcgcgggtgt tcgaactgcc cattcgggag cggacggagg ttcgcgcgct
      961 cggctcccga ccctcgggcg ggtacgtcct gtcggcggga tcgacggagt tccacgccga
     1021 cgccgtggtg atcgcctcgg ggagcatgag caggccccgc gttccggcca tggcggccaa
     1081 cctgccagac gacatcgcgt gcctttcggc cggaacgtat cacaatcccg aggccttgcc
     1141 cgacggggcc gtggtcgtcg tcggcagcgg tcagtccggg tgccagatcg cggaggatct
     1201 gctcgaagcc gggcgggagg ttttcgtctg cgcgagccgc gtcggccgga tcccgcggac
     1261 ctaccgcggg caagacattt tccactggct gcggcacatg aacttcttcg aggcccggcc
     1321 ggaagacctc gacgaccagg cgatgcggta tgcggctcag ccgcaggtgt ccggccgcta
     1381 cgggggccat acggtcagtc tgcagtcgct ggccagggac ggcgccacgc tgttgggacg
     1441 tgtcgagggc gtggacgggc atgtactgtc gcttggcagc aatctgcgcg aatgcgtcgc
     1501 gttcggcgac gaaatctcgc gcatgatcaa gctgggcatc gacggctaca tcgagcgtgc
     1561 cggcatccag gcggaaccgc cgaccccgga cccgggagag ccggccctgc cggatctcaa
     1621 ggggagcgat tcgtggcgat cgttcgatct gcgcgcgaac ggcgtatccg ccgtcgtctg
     1681 gtgtaccggg ttcgatgccg actggagctg ggtgaacctg gacgtcttcg acgatcgagg
     1741 cgagttccgg cacgatgacg gcataacgga cagcccgggg ctgtacgtct gcggattccc
     1801 gtgggtctcc aagcgaaaga gcgggctgct gtacggcgtc gccgaggatg ccgaacgtat
     1861 tgtccggcat atcgagagct acctcggcgc gaaggcaggg cgcccgtcat gagcgtcaga
     1921 gccggctggt ccactcccct cctgaacgtc gagagcatcg agcgttcgat accgttctac
     1981 agggaactcg gcttcgagac gatcgatgtc gagggcgagg ccggctgcct gggctgggct
     2041 cgcctgcact gcgagggtgg cgcgatcatg ctcctgcgct cggacgagcc gtcgccatcg
     2101 accgaggatc gattcctcct gtacctctac acgccggacc ttccggcgct tcgcgcccgc
     2161 ctcgtcgcgg ccagtgtcga ggtgaccagg attcaacatc cagagcacat gcgaagcgga
     2221 gagatgtctt tccgggaccc ggacgggtac accatcctgg tcggccactg gggcgagacc
     2281 gagcacgagg cctggctcag gcggatcgag gagaagcgcg ctgcgggatt gatctgaggg
     2341 gcactttccg ggccgacgcc tccctcaccc tcccggcttc cacccgacca cggtcacgct
     2401 cagcagcgct cccccgctct cgcggtaggc gcggacctcg gccaggtcga cgacaccctc
     2461 gagcgcgtcg tccggaacgt cgacgatctt ctcctcgacc acccgcacgt ccgcgaatcc
     2521 ggcgtcctcc agcatccgga catacgcctc ccggtcgatc gcccccgcga cgcacccggc
     2581 gtagagctcg gcgacgccgc gcaccgaggc cgggagctcg cctcgcgaga cgatatccga
     2641 gacgcagaag tgcccgccgg ggcgcagcgc gcggaacatc tcctcgaagg ccatcgcctt
     2701 gttcggcacc aggttcagga cgcagttcga gacgatgacg tcgacctccg cgtcgtcgac
     2761 gggcagcgcc tcgatgtcgc cgagccggaa ctcgacgttc cgggccccga tgaggtcggc
     2821 gttcgcgcgg gcccgctgga tcatggcggg cgtcatgtcg acgccgacga cccggccctt
     2881 ctcgccgacc aggcttcgcg ccacgaaggc gtcgttgccc gcgccggagc ccaggtccag
     2941 cacggtatcg cccgggcgga tgcccgcgtg ctcggtcgga aggccgcatc cgagaccaag
     3001 gtcggcctcc ggcatgtagc cctcgagggt cgtgtagtcg tcggacaggt ccacgggcac
     3061 ggtctccgaa ccgcagcagg aactgccgca gcacgaggac tgggtctgga cgatctcggt
     3121 gtacttgccg cggacgatgt ccttgagctc cgtcggtgtc ggcgtggctt gggtgttcat
     3181 attgatattc ttcgatatgg tgagcgaaaa aaagggtcag ccggtcaggc cggccacctg
     3241 gcgggccagt gcgccgatcg cgctcctgcg cgggctgtaa tggacccacg gaccacggtg
     3301 ctcggtctcg acgagacccg cctcgcggag caggcgcagg tggtgagaga tcgtcggctg
     3361 ggacaggtcg aagcgggcct cgacgtcgca ggcgcagacc gagcctccgg cgccgcccag
     3421 gatgtgcatg atcctgaggc gcaccgggtg cgagagtgcc ttcagcgccc gggacacctc
     3481 gtccaaggtc tcgtccggcg gaaggggcac gtcgcgcggg gcgcagcagg cgcctgcctg
     3541 ggtgatcctg gctatggtcg tcgatgggga cacggcggga gtataagcgt catattgaag
     3601 tttgtcaata tgtcgtcctg ccgacgggat cgattttctg tttcgcagca agcgtgcgcc
     3661 cgactcgggc tacgtcattc gcctggagtc cggcagacgg acggcacccg cccaccctac
     3721 tctcccccgt acgcccccac cgcgccttcc gcctcctccg cgctccaacc cagccattca
     3781 cgcacatccg ggggcatcgc gacttcccgg ctcgccggca gcgcgcggcg cagctcgtac
     3841 cggccgaggt ccggggcgat gaacacgctt cgcgcgtcgc cctccaattc ctgcccgtgc
     3901 gcttcgaagc ccgggacctg ctccttgaac gcctccagcg actcgaatcc ggcggtacaa
     3961 ccggcggcgt cggcatcgac ccaggtgacg atgggacccg cgagcgactc gtacggcgaa
     4021 gccggcctga cgtacacgtt gccgtccatc gcggccaacg ccggttccgt caactggtcg
     4081 cacagatacg ggggctggcc cacgcccagc agcgtgacac cgggccggct cgtcaccacc
     4141 aggttcttca ggaagacgtg accctcgcgc tcggtcactc ccggccccgt cgcggggtgc
     4201 cagttgaagt gatcgccctc ggcactgcga ccgtcgcgcg agaaacggac gggactgtcc
     4261 acgtaggtgt tgttgtagag gtgggcgtcg gccgagttca ggatccagga tcccagcccg
     4321 ttgtccacga aca
//
LOCUS       DBNHQZ010000264         5343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1029813, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000264 DBNHQZ010000000
VERSION     DBNHQZ010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1029813"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="AC16YU_07140"
     CDS             <1..472
                     /locus_tag="AC16YU_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6065036.1"
                     /translation="PAQVSVDTRPLGAALGDFGVSIDALQVNHPGRCYGYRVWIGDRS
                     FVFMPDNEIGACGPERACIEKLAAFCEGTDLLVHDAQYTEAELEIRRGWGHSTVAEAC
                     GLAAEAKVRRLLLFHHDPERSDAAVDDLVDRARLLLTFSEIDCGAASEGLTVRI"
     gene            701..2086
                     /locus_tag="AC16YU_07145"
     CDS             701..2086
                     /locus_tag="AC16YU_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065037.1"
                     /translation="MTVLFTGARRLIPAFLAMLLASFAIGCDGLGASAPDSAPRDSAP
                     APKTFEEGIPREASFTDKGGATWTIAFDETTFGGQDTDFSYTVSTSGAPSTGSFLALE
                     LPACATFVSSSPPGTMNANANGTLEWHFNDLAWVDGEAPVTYTFAGDVQIGLVRVSFK
                     SGGTGSAVETKVIAGPCQGRYPVSGHVLLDDHDGTMYGIAGVVVTLREDSDGDGLVDT
                     PNARNDGSVDDSRTTDADGRYAFDVWGPPFHSGIYEVSIGSEGANAVLHDTRLFTALT
                     PLSVVTDVKTSSAVDFKFEVNVAGALDAFDQGTVASDGQPRPYWRVQATGKGSPKYQP
                     EISFAEFEALLDGFSDAFDVSFAFGPDRISRIEAAGILQTASNGDVLEALRTGILVLG
                     LNYASGRGMCTGTNVIDGRCETWDEPATRLILEHAIGTLRNTGYQVAAKAPSGSTETT
                     TRLVSEVNNDG"
     gene            2155..>5343
                     /locus_tag="AC16YU_07150"
     CDS             2155..>5343
                     /locus_tag="AC16YU_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6065038.1"
                     /translation="MWTSIVVLAGLGPAPQSRAQSLPFRHYTTESEVAPLPSAAARRV
                     YQDSDGFVWIAVFSSGLVRYDGEETNLFTTADGLRDLNLSEIAQDADGRLWIGSDGGV
                     VASDRPLPEYGPGVPLRFEPTFRGHSLPATAVRERCMARSPEGGILVATAGALLHFRP
                     GPGEVSGPDTLLATGDRTIQAIGVTPDSSIWVSTDRGELLRAPDASAGFVTEARLPAI
                     ASAFQPEPDGSLLVGTRRGSIVRLRFRPLRTETVVQGIHGIVVDLAAGSNQDLWIATQ
                     GAGVVRVPMDERGAGLGVAPVSGLLGTDAYDLERDREGNLWIAQMGGLSRLPYNFDAF
                     SAFEAREIDDEPAALPEPYVLSVLPPDRSGILLAGTLGGGLTAIDAVGRSSVLPESAG
                     FPSERINALLGMDDGTVWVGTFRGAGVLVPPGSPLPVGAEASGTFTAGGIRYRTAVVG
                     RASGTVEAVSSVRLSDGSASRQTVWLSGQDAVTVYDGEHWTVLGATRGLPPSLFRGAA
                     ADSLGFIWIGTMDAGLYRSTAPVGLSGLGDVRFEAVWTRESGAPSDEIESLLFADGLM
                     WVGTVAGLTGIDPSTLGPVVQLGQDEGMPGEYATSMARSPATGSIWVGTAAGLAEVDP
                     RGRKVLRSVTRADGLVDDETCYYGSVRAGPDGTVYFGTCAGLAVYRPHRDRANDVPPT
                     PRVMELGYEENAWGANELAIRYAALSFANERKVRYRTQLVGFDENWSEETHEARIRYT
                     NLGAFFLPKTYTFQVTASNDVGLWAAAPAAIEVRVHPAWYLRWWAVLFWIGLVTGGSV
                     SYVRYKTRQQAIQLEKERKINEQLRKVDKLKDEFLANTSHELRTPLNGIIGIVEGLLD
                     GVAGDLTGMMRTNLATVVASGRRLASLVNDILDFSKMAEHDVVVQRKPVNVRILADIV
                     LQISTPLLAGKPVELRNEITPDLPPAAADESRVQQILHNLVGNAIKFTHEGRVTVSAE
                     ERDGMLAISVSDTGIGIPEDRRGAVFESFEQVDASTSREYGGTGLGLSITRRLVEAHG
                     GTIDFTSVLGEGSTFTFTLPVSEESVDDDEAER"
BASE COUNT          835 a         1794 c         1820 g          894 t
ORIGIN      
        1 gccggcccag gtgtcggtgg acacgagacc gctcggcgcc gcgctcgggg acttcggtgt
       61 ctcgatcgac gcactgcagg tgaatcatcc gggtcgctgt tacggctacc gcgtatggat
      121 cggggaccgg tccttcgtct tcatgccgga caatgagatc ggggcctgcg ggccggagcg
      181 ggcctgcatc gagaagctgg ccgccttctg cgagggcacc gacctcctgg tgcacgacgc
      241 gcaatacacc gaggccgaac tcgaaattcg acgcggctgg ggacacagca ccgtcgcaga
      301 agcgtgcggc ctcgccgcgg aagcgaaggt ccggcggctc ctcctgtttc atcacgatcc
      361 cgagcgctcg gacgcggccg tggacgacct cgtggatcgg gcccggctgc tcctgacgtt
      421 ctcggagatc gactgcggcg cggcgtcgga aggactgacc gtccggatct gacgaccggg
      481 cggtgtaggg tattttctga tgccccgggc tgcttttggc gccaaggacg accggccatt
      541 cccctacgcc ggatcgaggt tgcccccggt agcaatcaga ggggggtttc cgctacctat
      601 tcgctcaatg ctggaccgcg ttctgccttt ccccgcgggg ggacgggata ggcttcgtct
      661 gcggtcgttc cctcctcgag ccacatccac gaggtgtttc atgaccgtgc ttttcaccgg
      721 ggccagacgg ctcattcctg cgttcctggc gatgctgctc gcttccttcg cgatcggctg
      781 cgacgggttg ggcgcctccg cgcctgattc ggcgccccgc gatagcgcac ccgctcccaa
      841 gacgttcgag gagggcatcc cccgcgaggc gtcgttcacg gacaagggtg gcgcgacgtg
      901 gaccatcgcc ttcgacgaga ccaccttcgg cgggcaggac accgatttct cctacaccgt
      961 ctcgacttcc ggagcgccgt ccacggggtc gttcctcgcc ctggaattgc ccgcctgcgc
     1021 caccttcgtt tcctcgtcgc ccccaggcac gatgaacgcc aatgcgaacg gaaccctcga
     1081 atggcacttt aacgacctgg cgtgggtgga tggagaggcc cctgtcacct acacgttcgc
     1141 cggcgacgtg cagatcgggc tcgtccgggt gagtttcaag tcgggcggca cgggctcggc
     1201 cgtggagacc aaggtcatcg ccggaccgtg ccagggccgc tatcccgtat ccggacacgt
     1261 gctgctcgat gaccatgacg gtacgatgta cggtatcgcg ggcgtcgtgg taaccctcag
     1321 ggaggactcg gatggggacg gcctggtcga tacgcctaat gcccggaatg acgggagcgt
     1381 ggacgactcc aggaccacgg acgcagacgg ccgctatgcg ttcgacgtgt ggggaccgcc
     1441 gttccactct ggaatctacg aagtgagcat cggcagcgag ggggccaacg ccgtgctgca
     1501 cgacaccagg ctgttcaccg cattgacccc cctctcggtc gtaaccgacg tcaagacgtc
     1561 ctcggccgtc gatttcaagt tcgaggtcaa tgtggcgggt gccctcgacg cgttcgacca
     1621 gggcacggtc gcctccgacg gccagccgcg gccgtactgg cgcgttcagg ccacgggaaa
     1681 gggcagtccc aagtaccagc ccgagatttc attcgccgaa ttcgaagccc tgctcgacgg
     1741 attctccgac gcctttgacg tgagctttgc cttcggtccg gaccggattt cccggatcga
     1801 ggcggccggc attctccaga ccgccagcaa tggagacgtg ctcgaggcgc tccggaccgg
     1861 catcctggtc ctgggtctga attatgcctc gggccggggt atgtgtacgg gcacgaacgt
     1921 gatcgacggc cgctgcgaga cctgggacga accggcgacc cggctcatcc tggagcacgc
     1981 tatcggcacg ttgcggaaca ccgggtacca ggtcgccgca aaggccccgt ccggctctac
     2041 cgagaccacg acgagactgg tcagcgaggt caacaacgac ggttgatggt cccagtccct
     2101 gccgaatgca ggttggtcaa ggcggggcgg gggccatggc ccctgccccg cttggtgtgg
     2161 acctcgatcg tcgtgctcgc cgggctgggc cccgccccgc agtcccgggc acagagcctg
     2221 ccgttccgtc actacactac ggaaagcgag gtggccccgt tgccgtccgc cgccgcccgt
     2281 cgcgtgtacc aggactcgga cgggttcgtg tggatcgcgg tcttctcgtc gggcctggta
     2341 cggtacgacg gtgaggagac caacctgttc actacggccg acggactgcg cgacctgaac
     2401 ctgtccgaaa tcgcccagga cgccgacggc cggctctgga tcggatccga cggtggggtg
     2461 gtagcgagcg atcgaccgct ccccgaatat ggacccggcg tccccctccg cttcgagccg
     2521 acgttccggg gccattcgct tcccgccacg gcggtccggg agcgatgcat ggcgcggagc
     2581 ccggagggag gcatcctggt ggcgacggcc ggagcgctgc tccacttccg gcccggccct
     2641 ggagaggtct cgggccccga caccctcctc gccaccgggg atcgaaccat ccaggccatc
     2701 ggagtcactc ccgacagctc catctgggtg tctaccgatc gcggtgaact gttgcgggcg
     2761 ccggatgcct ccgccgggtt cgtgaccgag gcccggcttc ccgccatcgc gagcgccttt
     2821 cagcccgagc cggatggaag cctcctggtc ggcacgcgcc gcggcagcat cgtacgtctc
     2881 cgctttcgac cgcttcgaac cgagacggtc gttcagggca tccatggtat cgtcgtggac
     2941 ctggctgccg gttcgaatca ggacctgtgg atcgccacgc agggcgccgg cgtcgtccgc
     3001 gtaccgatgg acgagagggg cgccggcctc ggtgtcgcac ccgtttcggg cctgctcggc
     3061 accgatgcct acgacctcga gcgggaccga gagggcaacc tgtggattgc ccagatgggc
     3121 ggtctttccc gacttccata caatttcgat gcgttctccg ccttcgaggc ccgagagatc
     3181 gacgacgaac cggcggcatt gcccgaaccc tacgtgctct ccgtcctccc acccgaccgc
     3241 tcgggcatcc tcttggccgg aacgctcggc ggcgggctca cggcgatcga tgcggtgggg
     3301 cgatcgagcg tccttccgga atcggccggc tttcccagcg aacgcatcaa cgccctgctg
     3361 gggatggacg acggcacggt ctgggtgggg acgttccggg gagccggggt gctcgtccct
     3421 cccggcagtc ccttgcccgt cggcgcggag gcatccggca cgttcacggc cggaggcatc
     3481 cggtaccgga ccgcggtcgt cggccgggcg agcggcacgg tggaagccgt ctcgtccgtc
     3541 cgcctctccg acggatcggc atcccggcag accgtctggt tgtcgggcca ggatgccgtg
     3601 accgtctatg acggcgagca ctggacggtg ctcggcgcga cgcgtggcct tccgcccagc
     3661 ctgttccgcg gcgcggccgc ggattccctc ggtttcatct ggatcgggac catggacgcc
     3721 ggcctgtacc ggtccacggc gccggtcggc ctctccggac tcggagacgt ccggttcgaa
     3781 gcggtgtgga cccgggaatc cggggcgcct tcggacgaga tcgagtcgct gcttttcgcg
     3841 gacggcctca tgtgggtcgg gaccgtggcc gggctcaccg gaatcgatcc gtccacgctg
     3901 ggcccggtcg tgcaattggg acaggacgag ggcatgcccg gagagtacgc gacgagcatg
     3961 gcacggtccc ccgcgacggg atcgatctgg gtgggcacgg ccgccggact tgccgaggtg
     4021 gacccgaggg gccggaaggt cctccgctcc gttacccggg ccgacggact cgtcgacgac
     4081 gaaacgtgct actacggatc ggtccgcgcc ggcccggacg ggaccgtgta cttcggcacc
     4141 tgcgcagggc tcgccgtgta ccgcccccat cgcgaccgcg ccaacgacgt gccgcccacg
     4201 ccccgcgtga tggagctcgg ctacgaggag aacgcctggg gtgccaacga gctcgcgatc
     4261 cggtacgccg ccctcagctt cgccaacgag cgcaaggtcc gataccggac gcagctcgtg
     4321 ggcttcgacg agaactggtc cgaggagacg cacgaggcgc ggatccggta cacgaacctg
     4381 ggggcgttct tcctgccgaa gacctatacg ttccaggtga cggccagcaa tgacgtgggg
     4441 ctctgggcgg cggccccggc cgcgatcgag gtccgcgtcc accccgcatg gtatctgcgc
     4501 tggtgggccg tcctgttctg gatcgggctc gtaacgggcg gctcggtctc ctacgtccgg
     4561 tacaagacgc ggcagcaggc gatccagctg gaaaaggaac gcaagatcaa cgagcagctg
     4621 cgcaaggtcg acaagctgaa ggacgagttc ctcgcgaaca cctcccacga gctgcgcacg
     4681 ccgctcaatg gcatcatcgg cattgtggag ggcctgctgg acggcgtcgc gggagacctg
     4741 accgggatga tgcggacaaa tctggcgacg gtggtcgcct cgggccgccg cctcgcgagc
     4801 ctcgtgaacg acatcctgga cttctcgaag atggccgagc acgatgtcgt cgtgcagcgg
     4861 aaaccggtga acgtccgcat cctcgcggac atcgtgctcc agatctccac gcccttgctg
     4921 gcgggcaagc cggtggaact ccggaacgag atcacgcccg atctgccccc ggccgcagcg
     4981 gacgagagcc gcgtccagca gatcctccac aacctggtcg gcaacgcgat caagttcacc
     5041 cacgaaggcc gcgtcacggt gtctgcggag gagcgggatg ggatgctggc gatctcggtg
     5101 agcgacaccg gcatcggcat tccggaggat cgccgcggcg cggtcttcga gtccttcgag
     5161 caggtggacg cctcgacatc gcgcgagtac ggggggaccg gcctgggact ctcgatcacc
     5221 cggcgcctgg tcgaggcgca cggtgggacc atcgacttca catcggtact gggcgagggc
     5281 tcgacgttca cgttcacgct tccggtgtcg gaggaatccg tggatgacga cgaggcggag
     5341 cgg
//
LOCUS       DBNHQZ010000265         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_892868, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000265 DBNHQZ010000000
VERSION     DBNHQZ010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_892868"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..303)
                     /locus_tag="AC16YU_07155"
     CDS             complement(169..303)
                     /locus_tag="AC16YU_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065039.1"
                     /translation="MPFPASIPSRRRHAPALTLPVVSGLHVDLLAILVVLSILLVLVQ
                     "
     gene            607..1278
                     /locus_tag="AC16YU_07160"
     CDS             607..1278
                     /locus_tag="AC16YU_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065040.1"
                     /translation="MNRNGHRSAGLSSRARSLLFAAVLTATGILPGAAQSVSWSVLIG
                     AGYTGWSGPYEANGRWAGSLMALSAEVKASGRLGVRFELGGAISEAGARNSTTLVHER
                     TQLGLAARAYPLGRAHKWSPFGEAGAAVWLGARCSALRSGAILGKRLECAYFQPDPGG
                     AGGTLRPNASGVSPFLGVGVRHRAFGFGVRYDIAGPRMFENDAGSLTARTIHFTIEWV
                     FRRGR"
     gene            complement(1398..2369)
                     /locus_tag="AC16YU_07165"
     CDS             complement(1398..2369)
                     /locus_tag="AC16YU_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR6065041.1"
                     /translation="MSAPTRRPGTLAREVRAACRRRNLAYSTEKAYLGWIRRFVRHHG
                     LRHPAELGEDHIREYLSHLATTRRVAASTQNQALNAIVFLYGNVLGLDLDEFGAFVRA
                     RRPRRLPVVLTAAEARQVLARIGGPVGLVARLLYGSGLRVAEAVTLRVKDVDFEYGLV
                     HVQAAKGRKDRKTMLPERLHRPLKDHLVAVRRIHQADLAAGYGQVPLPYAFARKSSTA
                     ARDWLWQYVFPSSVRTTDLAYGARTRHHVSPSTVQKAVKAAARQAGVDKRVTCHTFRH
                     SFATHLLESGCDIRTVQELLGHGDLRTTMIYTHVLNRGVHVRSPLDP"
BASE COUNT          425 a          941 c          930 g          471 t
ORIGIN      
        1 gtccgcctcg tcacggatct gaccggtcgc ccggaggaga ctccggtgcg gtgctcgctc
       61 gaatcccttc tttacggtgt cgctgcgcat ggggtcgggg cctgtcgggt gggccggtcg
      121 acccggcctc cgatgctgcg aggcgacgaa tacgggtcga ccagggtgct actgtacgag
      181 cacgagtaga atcgaaagca ccacgagaat cgcgagcagg tcgacgtgca gaccggaaac
      241 cacggggagc gtcagggccg gcgcgtgccg gcggcgggac ggtatggagg ccgggaaggg
      301 catgaagcgt gggctgcacg gtttcggaga agtggccgga tggagccggc aggcgcctct
      361 ccttgtttcg ctgccagcga aatatcggca attaatccgt aggggcaagg atccggattc
      421 ttgaattttt cggaagcgct accggaagtg gcgaactcat gccgaccctc acgcgtgccg
      481 ttcggtcaga ccgcgcctct gttttggccg tgcgtcctcg tgctctacct tcggcgcctg
      541 cgttcgcccc ctgatcgatt cggccccttt gccccaacct taccgtccac tcacacgccc
      601 ccgaacatga accggaacgg acatcggagc gcagggctct cttcgcgtgc ccgctcgctc
      661 ctgttcgccg ccgtcctgac ggcaaccggc attctccccg gcgcggccca gagcgtgagc
      721 tggtccgtcc tgatcggtgc gggctacacc ggatggtcag ggccgtacga ggctaatgga
      781 cgatgggcgg gttcgctcat ggcgctttcc gccgaagtga aggcttccgg acgcctgggc
      841 gtccgattcg agctgggcgg ggccatcagc gaggccggtg cccgcaactc gacgacgctc
      901 gtgcacgagc gtacccagct gggcctcgcg gcccgcgcgt atcccctggg gcgcgcacac
      961 aaatggtctc ccttcggcga ggccggcgcc gccgtatggc tgggcgcccg gtgcagcgcg
     1021 ctgcgatccg gcgccatcct tggaaagcgg ctcgaatgcg cctatttcca gcccgatccg
     1081 ggaggtgcgg gaggaacgct caggcccaac gcgtcggggg tcagcccctt cctcggggtc
     1141 ggcgtgcgtc accgagcctt cgggttcggc gtgcggtatg acatcgccgg accccgcatg
     1201 ttcgaaaacg acgcaggctc gctcacggcg aggaccattc atttcacgat cgagtgggta
     1261 ttcaggcgcg ggcgttgagg gcgaaggtga ccctcgggcc tgtccggcat ggactcccgg
     1321 gattgatccg ttcggtgccg gtcctgaccg ggccgcgcga cgggcgcctc gcctccgcag
     1381 gcgcatgagc ccggcggtca cggatccagc gggctcctga catgcacgcc gcgattcagg
     1441 acgtgcgtgt agatcatcgt ggtgcgcagg tctccgtggc cgagcagctc ctgtaccgtg
     1501 cggatgtcgc agccggactc gagaaggtgg gtcgcgaagc tgtgccggaa cgtgtgacac
     1561 gtgacccgct tgtcgacccc ggcctgccgc gccgccgcct tgaccgcctt ctgcaccgtg
     1621 ctcggcgaga cgtggtggcg cgtcctcgcg ccgtaagcca ggtcggtcgt gcgcaccgag
     1681 gacgggaaga cgtactgcca gagccagtcg cgggccgccg tcgaggactt ccgggcgaag
     1741 gcgtacggaa gcggcacctg cccgtacccc gccgccaggt ccgcctggtg gatccgccgc
     1801 acggcgacga gatggtcctt caatgggcgg tgcagcctct cgggcagcat ggtcttccga
     1861 tccttccggc ccttggctgc ctgcacgtgc acgaggccgt attcgaagtc cacgtccttg
     1921 acgcgcaacg tgaccgcctc cgcgacgcgc aggccggagc cgtacaggag ccgggccacg
     1981 agccccaccg gaccgccgat ccgcgcgagc acctgccgcg cctcggccgc cgtgagcacc
     2041 accggcagcc gcctcggcct gcgcgcgcga acgaacgccc cgaactcatc caggtcgagc
     2101 ccgagcacgt taccgtacag gaagacgatc gcgttcagcg cctggttctg tgtggaagcc
     2161 gcaacccgcc gcgtcgtggc caggtgcgag aggtactcgc ggatgtggtc ctctccgagc
     2221 tccgccggat gtcgcagacc gtggtggcga acgaatcgcc ggatccagcc gaggtaagcc
     2281 ttctccgtcg agtatgcgag gttgcgccga cggcatgcgg cgcgcacctc acgcgccagc
     2341 gtgcccggcc gccgggtcgg ggccgacatg gcatccggtt tccctgtaga cccgcgcgcc
     2401 cccctccccg taccctcgga cagccatggc gatatgcggg cgcggcgcta caccactcga
     2461 ctcccggcga atatgcggtc cggtgatatg ccgccaggcg ccacgtgaca tccctatcgc
     2521 ttgacgcaac tcgatgcagc cattacactt catcgacccg cgaccctcca attcctctgt
     2581 tggacgggcg cgacgtgccc gtagaagaca ggttaggcct caagagacgt gacgggcctc
     2641 atgtcaatac tactcgtcgt cgtcgggata attctcggct tggcgatctg gtttggcccc
     2701 atagttctcg cggtgcgtcg gaagcaccca cgcctcggaa tgatcattgc gctgacgtgt
     2761 ctcggtc
//
LOCUS       DBNHQZ010000266         6338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_485168, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000266 DBNHQZ010000000
VERSION     DBNHQZ010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_485168"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC16YU_07170"
     CDS             <1..351
                     /locus_tag="AC16YU_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6065042.1"
                     /translation="GLGVNWALEAATEYAGRGRSIEVVDLRTLVPWDRETVLGSVRKT
                     GRLLVLHEAARTGGFGGEVASSIAEEAFEWLDAPPVRVAGRDLPIPFARHLEEIVHSA
                     RPRLRPSLDRLLAW"
     gene            414..1835
                     /locus_tag="AC16YU_07175"
     CDS             414..1835
                     /locus_tag="AC16YU_07175"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR6065043.1"
                     /translation="MSLRMPGIPHAVAATTISPARPAPGPVISAVLAMALLAACGGGA
                     EPGGRAHLAGSFSVAEALDAGGDLGGFEVVVLATPGGELDTLAVAVSDSAGSFAGTVR
                     VPERGVYPIVISRAGRRLHIDEIVLADGDSIRLRAEFPLDARRLRIVSRENSAWSAYR
                     NAKALYNRSVLELLESGTYTDFTMRRTATQSATILWSLRELYPGTLGATVAETEAVVL
                     LESWNDSLAVARFRSLGAEHPGIVALASAARRSTARLEGQEAAIALLRSIADSVSSAD
                     ALAIRSEIVLARRDSMQRTEALAEARSLLADGRGTEWERWAGNAIYELENLMPGMPAP
                     AFSAIDVNGMPVDLGRFAGRTLLLEFFRPGDETYLRELPARRALLDALRGRRFAALSV
                     SVEPDSALNEALLDGRNLLGTFVFDPDGLEGSLARAYNVNLVPTRVLIGPDGRLISRY
                     TGPAIGAVRNDLAGIFGSGFAGR"
     gene            1888..2646
                     /gene="tpiA"
                     /locus_tag="AC16YU_07180"
     CDS             1888..2646
                     /gene="tpiA"
                     /locus_tag="AC16YU_07180"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6065044.1"
                     /translation="MLVAGNWKMHTDRTSARQLAAAVVSAVGHPDRVHVAVCPPFVSL
                     DAVFTVLHGSPVRLGGQDMHHEDSGAFTGAVSAPMLRSVGCHYVIIGHSERRQFFGET
                     DEGVNAKVHRARAHRLVPIVCVGETLEERDAGREREVVGRQVDRGLADVRLADAGELV
                     VAYEPVWAIGTGRTATPEQAADMHAFIRERLVGLLGPGTASDVDILYGGSVKPDNATD
                     LFTRPHVNGGLIGGASLKADDFAAIVAAAVSAAK"
     gene            complement(2653..2949)
                     /locus_tag="AC16YU_07185"
     CDS             complement(2653..2949)
                     /locus_tag="AC16YU_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6065045.1"
                     /translation="MPTYTYRRADGTTFELEQRITEAALDTCPTTGQPVRRVITAGAG
                     LVFKGSGFYLTDYARRNSSPSTGSDTPGTSDGTTTGEGSAEKAAPATGNGKATG"
     gene            complement(3039..5966)
                     /locus_tag="AC16YU_07190"
     CDS             complement(3039..5966)
                     /locus_tag="AC16YU_07190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065046.1"
                     /translation="MRTLLTGLSLAIAILSPLPCGCGPPPVAPAPAVTDQTADMDLDE
                     EGSRWADEVLESLDLEERVAQLFAVSVSGEFESDDPEARLEKIRFVRDLGIGGVIFFQ
                     GTPLEQAAFANELQSLARVPLLVSQDMEWGAGMRLEGATRFPVAMAIGATGDPGLAYR
                     AGLATALEAQALGVSQVFAPVADVNTNPLNPVIDTRAFSDRSDRVAVFVEAYVRGLQD
                     GGVLATIKHFPGHGDTDGDSHDELPVSRLAAADLDTLHLAPFRAAIAANVASVMTAHV
                     AYPALDSGRTRPATLSPPVVRGLLRNRLGYGGLIVTDALNMSAVSRLAPPGEVAVQAL
                     EAGVDMLLMSVDVELAHRTVVEAVRGGRLDSTRIERSVRRILRAKAAAGLHHRRSADL
                     DRVRTAVGGGPARALAETIAERSLTLLAGRLPILTGRPGERLLVVSVSDRTGDVPDPA
                     FRDALAEENPDASISFVSVDGSSIAASRTAIARYLAEADRVVVAVHAFAGRWKKRPRV
                     AAALAGVVDGLTSGSKPATLVLFGTPYVLTQLSRLPDRILLAYDSGEAMQRAAAGALS
                     GSVTVTGRLPVTIPSAFAFGAGISSPAVYPRRTSPEAAGMDGETLAHVDSLIQDALDR
                     HAFPGAAVAVGRGSALAYLEGHGYYTYRSTRKVTPDAVFDLASLTKVVATTTVMMRLY
                     EDGLVDLDAPVARYVPEFGQAGKQRVTVRQLLSHTGGLIPYRAFHRLGITTRRDVMAA
                     IYGDSLQYEPGTESRYSDFGPIVLAELVERITGRPFADVARTEVFEPLGMWHTGYRPS
                     GAFDPAAIPTEVDDYFRHRTLQGEVHDETAWILGGTAGHAGLFSTARDLARFASMLVH
                     EGRVGGRPFLQPETIRLFTTPIDPTGGHTRALGWDTRSFDARSSAGQLFGPRSFGHTG
                     FTGTSMWIDPDARLFVILLANRVYPTRANSGHVAVRPALADIAYSALRSPRIEPLLQS
                     PAD"
     gene            6140..>6338
                     /locus_tag="AC16YU_07195"
     CDS             6140..>6338
                     /locus_tag="AC16YU_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6065047.1"
                     /translation="MPSSERGGYLLLTLFLGVLMAALDIAVLGPAIPAVRAEFGIDER
                     TVAWAFNTFVFFNLLGVPFMSK"
BASE COUNT          922 a         2219 c         2284 g          913 t
ORIGIN      
        1 ggacttgggg taaactgggc gctcgaggcc gccacggagt acgccgggcg cggccgatcg
       61 atcgaggtgg tggacctcag gaccctggtc ccctgggacc gggagaccgt gctcggttcc
      121 gtgcgcaaga cgggccggct cctggtgctg cacgaggcgg cgcgcaccgg cggattcggc
      181 ggcgaggttg cttcttcgat agccgaggag gcattcgagt ggctggacgc tccgcccgtg
      241 cgcgtcgccg gccgcgacct gccgattccc ttcgcccggc atctcgagga gatcgtgcac
      301 tccgcgcgcc cccggctgcg cccgtcgctc gatcgactcc tcgcctggtg agcgcgggat
      361 ccgatgcggc gcccggtcgt tgcgatcgcc ccgaatggag aggcgatctt cccatgagtc
      421 ttcgcatgcc aggcattccg cacgccgtcg cggcgaccac catctccccg gcacgcccgg
      481 ccccgggacc cgtgatctcg gcggtgctcg ccatggcact gcttgccgcc tgtggtgggg
      541 gcgccgagcc gggcgggcgg gcccacctgg ccggctcgtt cagcgtggcg gaggcgctcg
      601 acgcgggtgg ggacctgggc ggattcgagg tggtcgtgct ggccacgccg ggcggagaac
      661 tggacacgct cgcggtcgcg gtatccgaca gcgccgggtc ctttgccggc accgtccgcg
      721 tgccggagcg cggcgtgtac cccatcgtca tttcccgggc cggtcggcgg ctgcacatcg
      781 acgagatcgt cctggcggac ggcgactcga tccgcctgcg cgccgagttc ccgctcgacg
      841 cccggcgcct gcggatcgtg tcgcgggaga acagtgcctg gtcggcgtac cgcaacgcca
      901 aggcgctgta caaccggtcg gtcctggaac tgctcgaatc gggtacgtac acggatttca
      961 cgatgcgccg gacggccacg cagagcgcca ccatcctgtg gagcctccgc gagctctacc
     1021 ccggaaccct cggcgccacg gtggcggaga cggaggccgt ggtgctcctg gaatcctgga
     1081 acgacagcct ggcggtggca cgtttccgca gcctcggcgc ggaacatccg ggcatcgtgg
     1141 ccctcgccag cgccgcgagg cggtccacgg cgcgcctgga agggcaggag gccgcgatcg
     1201 cgctcctgcg gtcgatcgcc gattccgtct cgtcggccga tgcgctggcg atccggtccg
     1261 agatcgtcct ggcccgccgg gacagcatgc agcggaccga ggcgctcgcg gaggcgcggt
     1321 cgcttctggc cgacggacgg ggcacggagt gggagcggtg ggccggcaac gccatctacg
     1381 agctcgagaa cctgatgccc ggcatgccgg cgcccgcctt ttcggcgatc gacgtgaacg
     1441 gaatgcccgt ggatcttggc cgcttcgccg gccgcaccct gctcctcgag ttcttccggc
     1501 ccggcgacga gacgtacctg cgcgagcttc ccgcgcgtcg ggcgcttctc gacgccctgc
     1561 gggggcggcg cttcgccgcg ctttcggtct cggtcgagcc cgattcggcg ctgaacgagg
     1621 ccctgctcga cggccgcaat cttctcggga cattcgtttt cgacccggac ggcctcgagg
     1681 gcagccttgc gcgggcctac aacgtgaacc tggtgcccac gcgcgtgctc atcggaccgg
     1741 atgggcgcct catctcccgc tacacggggc cggccatcgg tgccgtgcga aacgacctgg
     1801 ccggtatatt cggctccgga ttcgcaggac gatgatccga cggggcgatt tccgccccgc
     1861 ccgaggacgc ctaacccagc cagaacgatg ctcgtagccg gcaactggaa gatgcacacc
     1921 gaccggacct ccgcgcggca gctcgccgcg gcggtcgtct ccgcggtcgg ccatcccgac
     1981 agggtgcacg tggccgtgtg cccgcccttc gtaagcctgg acgcggtatt caccgtgctc
     2041 cacgggagtc ccgtgcgact cggtggccag gacatgcacc acgaggacag cggggcgttc
     2101 accggggccg tctcggcccc gatgctgcgc tcggtcggat gccactacgt gatcatcggc
     2161 cactcggaga gacgccagtt cttcggggag accgacgagg gcgtgaatgc gaaggtgcac
     2221 cgagcccggg cgcatcgcct ggtgcccatc gtctgcgtcg gtgagacgct ggaggagcgg
     2281 gatgccggcc gggagcgcga ggtggtcggc cgccaggtgg accgcgggct cgcggacgtc
     2341 cgcctcgccg acgccggcga gctggtcgtg gcatacgaac cggtctgggc gatcggcacg
     2401 ggccggaccg cgacgcccga acaggccgcc gacatgcacg cattcatccg ggagcgcctc
     2461 gtcggactcc tggggccggg caccgcttcg gacgtcgaca tcctgtacgg cggctcggtg
     2521 aagcccgaca acgcgaccga tctcttcacc cgcccccacg tgaacggggg actcatcggc
     2581 ggcgcgagcc tgaaggccga cgacttcgcg gcgatcgtcg cggcggcggt ctcggcggca
     2641 aagtagcccg cctcaacccg tggcctttcc gttgccggtg gcgggcgcgg ccttctcggc
     2701 cgatccttcg cccgtcgtcg tgccgtcgga cgtacccggg gtgtcggaac ccgtggacgg
     2761 gctcgagttc cgccgggcat aatccgtcag gtagaagccg cttcccttga acacgagccc
     2821 ggcgcccgcc gtgatgaccc gccggacggg ctgcccggtg gtgggacacg tatcgagggc
     2881 ggcttcggtg atgcgctgct ccagctcaaa ggtcgtgccg tcggccctgc ggtacgtata
     2941 ggtcggcatg atgtgcggat gacaggatgg caggaggtgg gcttcgaagt ccaccgttca
     3001 aattctgttc cgtatccggt cggcgcgggc cccgggcctc agtctgcggg tgactgcagg
     3061 agaggctcga tccgcggtga gcggagcgcg gagtacgcga tgtcggcgag cgccggccga
     3121 acggcgacgt gccccgagtt cgcgcgcgtc gggtacacgc ggttggcgag caggatcacg
     3181 aagagccggg cgtccggatc gatccacatg gacgtgccgg taaagcccgt gtgcccgaag
     3241 ctcctggggc cgaagagctg gccggcgctc gagcgggcat cgaagctccg cgtatcccac
     3301 cccagggccc gcgtgtgtcc acccgtcgga tcgatcggcg tcgtgaagag ccggatggtt
     3361 tccggctgca ggaacgggcg gccgcccacg cgcccctcgt gcacgagcat cgacgcgaag
     3421 cgggccaggt cgcgggccgt cgagaagagg cccgcgtggc cggcggtacc gcccaggatc
     3481 catgccgtct cgtcgtgcac ttcgccctgg agcgtccggt gccggaaata atcgtcgacc
     3541 tcggtcggga tcgcggcggg atcgaaggcg cccgagggtc gatagccggt gtgccacatg
     3601 cccagcggct cgaagacctc tgtccgggcg acgtccgcga agggtcggcc cgtgatccgc
     3661 tccaccagtt cggcgagcac gatcgggccg aaatccgagt agcgggactc ggtgcccggt
     3721 tcgtactgga gtgagtcccc gtagatggca gccatgacgt cccggcgggt cgtgatcccg
     3781 agccggtgga aggcccggta ggggatgagc ccacccgtgt gcgagaggag ctgcctcacc
     3841 gtgacgcgct gcttcccggc ctgcccgaat tccggaacgt accgcgccac aggggcgtcc
     3901 agatccacca gcccgtcctc gtacaggcgc atcatgaccg tcgtcgtggc caccaccttc
     3961 gtgagcgagg cgaggtcgaa gacggcgtcg ggagtgacct tccgggtgga ccggtacgtg
     4021 tagtacccgt ggccctcgag gtacgcgagc gcgctcccgc gtccgacggc caccgccgcg
     4081 ccggggaagg cgtggcggtc gagggcgtcc tggatcagcg aatccacgtg ggcgagcgtc
     4141 tcgccgtcca tgcccgcggc ctcgggcgag gtccgccggg ggtacacggc gggggacgag
     4201 atgcccgcgc cgaacgcgaa ggccgacgga atcgtgacgg gcagccgacc ggtgaccgtc
     4261 acgctccccg agagcgcccc ggccgctgcc cgctgcatgg cctcgccgga atcgtaggcc
     4321 aggaggatgc ggtcgggaag ccgggaaagc tgcgtgagca cgtacggcgt tccgaagagg
     4381 accagggtgg caggcttcga gccggaggtc aggccgtcga ccacgccggc gagcgcggcg
     4441 gcgacccgcg gccgcttctt ccaccggccg gcgaaggcgt gcacggcgac gaccacgcgg
     4501 tcggcctccg cgagatacct ggcgatggcg gtcctggacg cggcgatcga gctcccgtcc
     4561 accgagacga acgaaatcga ggcgtccgga ttctcctcgg ccagggcatc acggaacgcc
     4621 ggatccggca cgtcgccggt ccggtcggag acggaaacga cgagcaggcg ctcgcccggc
     4681 cggccggtca ggatcggcag ccgaccggcc agcagcgtga ggcttcgctc ggcaatcgtc
     4741 tccgcgaggg cccgggccgg cccgccgccg acggccgtgc gcacccggtc caggtcggcc
     4801 gagcgccggt ggtggagccc cgcggccgcc ttggcccgca ggatgcgccg gacgctgcgc
     4861 tcgatgcgcg tcgaatcgag ccggccgccc cggacggcct cgaccaccgt ccggtgcgcg
     4921 agctccacgt cgacgctcat cagcagcatg tcgaccccgg cctcgagggc ctgcaccgct
     4981 acctccccgg gcggcgcgag acggctcacc gccgacatgt tcagcgcgtc ggtcacgatg
     5041 agcccgccat accccaggcg gttcctcagc agcccgcgaa ccacgggcgg agagagggtg
     5101 gccgggcgcg tccggcccga gtcgagcgcc gggtaggcca cgtgggccgt catcacgctg
     5161 gccacgttcg cagcgatcgc ggcccggaac ggagccaggt ggagcgtatc gaggtcggcc
     5221 gccgccaggc gggacaccgg aagctcgtcg tgcgagtcgc cgtccgtatc accgtggccc
     5281 gggaaatgct tgatcgtggc cagcacccct ccgtcctgca ggccgcgcac gtatgcctcg
     5341 acgaacaccg ccacacggtc cgaccggtcg gaaaacgccc gcgtgtcgat gaccgggttg
     5401 agcgggttcg tattgacatc cgcgaccggg gcgaagacct ggctcacgcc cagggcctgg
     5461 gcctcgaggg cggtcgcgag ccccgcccgg taggccagcc cagggtcccc cgtggccccg
     5521 atcgccatgg cgacggggaa tcgcgttgcg ccctcgaggc gcatgcctgc gccccactcc
     5581 atgtcctgcg agacgaggag cggaacacgg gccagcgact ggagctcgtt cgcgaaggcc
     5641 gcctgctcga gcggggtgcc ctggaagaag atcaccccgc cgatgcccag gtcccggacg
     5701 aaccggatct tctcgagccg ggcctccgga tcgtccgact cgaactcgcc gcttacggac
     5761 accgcgaaaa gctgcgcgac ccgctcttca aggtccagcg actcgagaac ctcgtccgcc
     5821 caccgacttc cctcctcgtc gagatccatg tcggcggtct ggtccgtcac ggccggcgcc
     5881 ggagcaacgg gcggaggacc gcagccgcac gggagcggcg acaggatggc gatggccagc
     5941 gacaggcccg tgagcagggt gcgcatggtt cgggacggat tccgggcccg gcaatctacg
     6001 ggaaccgggg cggcccagaa aggccgacgg agtggtctgc cgcccgcgtg cggacgccgg
     6061 accgagcgac gcaaccgggg cacgacaaag gggtagaatg cgccgcacgt cccctttcgt
     6121 ccgatcagtc caagccggca tgccgagcag cgaacgcggc ggatatctgc tcctcaccct
     6181 gttcctcggc gtgctgatgg ccgcgctgga catcgcggtc ctcggtccgg caattcccgc
     6241 cgtccgtgcc gagttcggca tcgacgagcg gacggtggcc tgggccttca acacgttcgt
     6301 gttcttcaac ctcctgggcg ttccgttcat gtccaagc
//
LOCUS       DBNHQZ010000267         9580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1840483, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000267 DBNHQZ010000000
VERSION     DBNHQZ010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1840483"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="AC16YU_07200"
     CDS             complement(<1..408)
                     /locus_tag="AC16YU_07200"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HIR6065048.1"
                     /translation="MPGFDPSARERLSTALKREAARLGFGACGIAEAGALDEEARQLE
                     AWLRAGRHGTMLWMERNVEKRLDPRKLVPGARSVVSVLHDYRPAAEPRRDAGFGRISR
                     YAWGDDYHEVMKERLAQLFDWLDHEAGGTGGRVF"
     gene            complement(589..843)
                     /gene="rpsT"
                     /locus_tag="AC16YU_07205"
     CDS             complement(589..843)
                     /gene="rpsT"
                     /locus_tag="AC16YU_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6065049.1"
                     /translation="MPQHKSAAKRVRQTERRRVYNRLHTSRVRTLIKKLRQTTDPSEA
                     SSLLGTVKAYLDRMASKGIIHKNKAANYKSDLERHVNTLG"
     gene            1003..1911
                     /locus_tag="AC16YU_07210"
     CDS             1003..1911
                     /locus_tag="AC16YU_07210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6065050.1"
                     /translation="MFSILSDFIRNAGERLTLIVLDEDGLGHPRRYNVRPVRLFAGIG
                     IAVAVVAALTVLVVVATPVRGWFPGLATDEMRQEARLNAARLQTVEDSLSAQEAWLTH
                     VRNLVLGRIEPGEGAGMPDLGRGTPFTGDLADMTAEPLSNDWQDHQQPALPVARLPAD
                     SMARLISVATAGDRFLSSLSFPMLPPVSGLLTRSFDARGGHYAIDLAVKEGTIVRAVG
                     DGYVFLADWTHDGGHVIAVQHAEGFVSVYKHNQQLLKRAGDRVQNREPISLSGNTGEI
                     TTGPHVHFELWLDGLAQDPADYLIGL"
     gene            1950..2456
                     /locus_tag="AC16YU_07215"
     CDS             1950..2456
                     /locus_tag="AC16YU_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6065051.1"
                     /translation="MARLPMETDTDAINLIGEGSEVEGVLRSSTDVRVNGRVKGEVNV
                     EGRVIVADKGVIDGTLIAANADVAGTVLGDLQVAERLLLKSTARVEGTIKTARLVVEE
                     GAMFDGQCDMGRLDRKRKDRIEKATAEAGPKPAADTGARPVGEAGSKPPSPTKSEFMF
                     DPGERKAG"
     gene            2503..2730
                     /locus_tag="AC16YU_07220"
     CDS             2503..2730
                     /locus_tag="AC16YU_07220"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR6065052.1"
                     /translation="MGLGLQLAGSVLFYVVAGYLADRWLGTEPWLLVAGAVIGMISFF
                     VLLARVVRRLNKETSRRAAERRQKAGKEARN"
     gene            2877..3185
                     /locus_tag="AC16YU_07225"
     CDS             2877..3185
                     /locus_tag="AC16YU_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065053.1"
                     /translation="MTDGTWMSALPGAAAGIAYGITSWWMVRRAVRRSPKTFLLLVMG
                     GMLLRLLAASIIVVAVLVWLPVHPAIFIGTFFGFFVIALVAEVITLHRQQRAAGPPTT
                     "
     gene            3209..4438
                     /gene="atpB"
                     /locus_tag="AC16YU_07230"
     CDS             3209..4438
                     /gene="atpB"
                     /locus_tag="AC16YU_07230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6065054.1"
                     /translation="MPTRLAFRLLALAGALALVAASPAAAQDHAEAEELDPVHHQVDG
                     HYLDFEPIGKVELPRIFVVRRADGSLALDLFGSSTAAVESGRYTAHFEEHGEAAAGEA
                     VDAAAGHAAGQRAAPQATAPDKVYEATLVPPDGGEIVLDLSWTKHLVFAWIGALIVLL
                     LFVRVGSRYRAGIGRDTEPRGLLQNLLETMVIFVRDEIARPNLGPKTDRYLPYLLTAF
                     FFILTCNLLGLVPFGATATSNIMVTAVLATFTFVLTQLGGSKDYWKHILWPPGVPTFV
                     KPILIPVEILGLFTKPFALAIRLFANMTAGHLVILSLIGLIFAFNNLFGPSAGYGVAP
                     VSVAFSLFIYLLEILVAFIQAYVFTMLSALFIGMAVEEHAHHDDGHGHDEVHALPDPS
                     RETIHIDIPERQPVAAG"
     gene            4488..4715
                     /gene="atpE"
                     /locus_tag="AC16YU_07235"
     CDS             4488..4715
                     /gene="atpE"
                     /locus_tag="AC16YU_07235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR6065055.1"
                     /translation="MEPTALAYLGAGIGAGLVAIGAALGIGRLAGPAMEGSARQPEAA
                     GDIRTSMIIAAALIEGVALFGLVICIILAFK"
     gene            4778..5287
                     /gene="atpF"
                     /locus_tag="AC16YU_07240"
     CDS             4778..5287
                     /gene="atpF"
                     /locus_tag="AC16YU_07240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6065056.1"
                     /translation="MEPILAANLLSPNFGLVIWIAITFFALLLLLRRFAWGPITSALV
                     AREDRIRESLEQAQKALAEARQIQADNDRARREADAEAQRLLRQSREAAEAIRSEEVE
                     RTREQIRQMQTSAQAEIARERDGALETLRSEVADLAIQAAERVLDANLDDDRQRKLVN
                     DFLGGLPKN"
     gene            5321..5875
                     /gene="atpH"
                     /locus_tag="AC16YU_07245"
     CDS             5321..5875
                     /gene="atpH"
                     /locus_tag="AC16YU_07245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR6065057.1"
                     /translation="MSQLTIARRYARALLEQAEAEGVTDPVDADVRAIRDAVAASREL
                     AAFLESPILSRERKQSVVRALFAGRVEDVTLRFLEMLADKRREGLFAMIADAYLEQRD
                     EQLGVVGVSVRTARTLDAAAEKAIARRIGEITGKESRLDVAVDASLMGGLVVRVGDTV
                     YDGSVLNRLESLRERLIHGTAANA"
     gene            5915..7504
                     /gene="atpA"
                     /locus_tag="AC16YU_07250"
     CDS             5915..7504
                     /gene="atpA"
                     /locus_tag="AC16YU_07250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6065058.1"
                     /translation="MATAIRPDEVTAVLKRELGGFTAETEVYEVGTVLQVGDGIARIY
                     GLSKVQAGELLDFPSSGITGMVLNLEEDNVGAVLFGDVDAVREGDEVRRTQRIASIQV
                     SESMLGRVIDPLGNPVDGRGALAGPRYEMPLERKAPSVIYREPVKEPLQTGIKAIDSM
                     IPIGRGQRELVIGDRQTGKTAVLIDTIINQKSTHQTDKPVYCIYVAIGQKNSTVAQVA
                     RSLEEHGAMEYTVIVNAGASTPAPLQYIAPYAGACIGEYFRDTGRHALVIYDDLSKQA
                     VAYREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKINSTDAVARQMNDVPESIREKV
                     KGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLESGLFNAGVRPAINVGISVSR
                     VGGNAQVKGMKKVAGTLRLDLAQFRELEAFSKFGSDLDPATQRQLRRGERLVEVLKQG
                     QYAPVPVEEQIAIIHVATQGLLDAIPVNRVKEFEREFIDKLRLRHQGILDGIRDTGAL
                     SDDAAATLKQAAGELVRAYTA"
     gene            7536..8459
                     /gene="atpG"
                     /locus_tag="AC16YU_07255"
     CDS             7536..8459
                     /gene="atpG"
                     /locus_tag="AC16YU_07255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR6065059.1"
                     /translation="MASLRDIRNRIASVQNTQQVTRAMKMVAAAKLRRAQEAIFRTRP
                     YAYKVGEIISHLKRHTDPGSHPMFIARGETRAVLLVVVTADRGLAGAFNTNIIKAAEQ
                     AIHERFAALHASGNLYLLCVGRKGHEHFQKRGFRLVGDFRFVFDHLEFGTANRIVHEA
                     LDGFTGGRWDEVHLVYNEFKNTISQNRIVEPFLPIRAEQFLTPVMEKELDHQEEEKVG
                     VAVDYIFEPRASTILEALVPRYLNYEVWRALLESNAAEQGARMVAMDNATSNASELIR
                     DLKLKYNRARQDAITKELIEITSGADALAGG"
     gene            8497..9147
                     /locus_tag="AC16YU_07260"
     CDS             8497..9147
                     /locus_tag="AC16YU_07260"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR6065060.1"
                     /translation="MEFLSDIDQKLVGLFEEYGLWVYVVLFAIAFLESTVTPFLPGDA
                     LVFAAGAVAAAGATTLPLMWVLFVGATVLGTMSSYELGRRVGPSAFRERRRFLNTEQL
                     EHARRFFDRYGGRTLAVGRFIPVVRTLAPVVAGILRMPYARFMGYTVLGCVLWVTLML
                     VSGYFVDTVPVLRARFGIVLAAVFLLSMVPPTIEWLWDHIGRRIYRAVLRRFGHEA"
     gene            9310..9398
                     /locus_tag="AC16YU_07265"
     tRNA            9310..9398
                     /locus_tag="AC16YU_07265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9344..9346,aa:Ser,seq:gct)
BASE COUNT         1518 a         3279 c         3275 g         1508 t
ORIGIN      
        1 gaagacccgc ccgccggtgc ccccggcctc gtggtccagc cagtcgaaaa gctgggcgag
       61 gcgctctttc atgacctcgt ggtagtcgtc tccccacgcg taccggctga tccggccgaa
      121 cccggcatcg cggcgcggct cggcggcggg ccggtagtcg tgcagcaccg agaccacgct
      181 gcgcgccccg ggcacgagct tccgagggtc gaggcgcttc tccacgttcc gctccatcca
      241 gagcatggtg ccgtgacgtc ccgcgcgcag ccacgcttcg agctgccgcg cctcctcgtc
      301 gagcgctccg gcctccgcga tcccgcacgc gccgaagccg aggcgcgccg cctcgcgctt
      361 gagggcggtc gagaggcgtt cgcgggcgga tgggtcgaag ccgggcacgg gtacagggga
      421 tttccgggcc aacgcccgcc gcgccgcccc ggttcgtccg tccatgtcgg gccggattcc
      481 gcagggcccg caggatcgcc gactccgggc gcgggccgcc gcggcggccg caatgaaaaa
      541 aggcccggac cgggtgggtc tgggcctttc ggaagggcac gaggcggatc agccgagcgt
      601 gttcacgtgg cgctcgagat cgctcttgta gttggccgcc ttgttcttgt gaatgatccc
      661 cttggacgcc atccggtcca ggtaggcctt caccgtgccg agcagggagg aggcctcgga
      721 cgggtccgtc gtctggcgga gcttcttgat caacgtgcgg acgcggctcg tgtgcaggcg
      781 gttgtagacg cggcggcgct cggtctggcg aacgcgcttg gcagctgact tgtgctgggg
      841 catggacggg tgggtccgct tgtaatcgaa tgcagagcct acgaatatac tgcagccgta
      901 cccgaggcgt caacgacgtg tcgaaagcgc tccgcgcggc ggggtggaac cccgctgctc
      961 gggcggtcgt ttcgggccgt gtaaagccgg ggcggctcgc ggatgttctc cattctctcc
     1021 gatttcatac ggaacgccgg tgagcggctc acgctcatcg tgctcgacga ggatgggctc
     1081 ggccacccga ggcggtacaa cgtgcggccc gtacgcctgt tcgcagggat cgggatcgcg
     1141 gtcgcggtcg tcgcggccct tacggtgctc gtggtggtgg ccacgccggt gcggggctgg
     1201 tttcccggcc ttgcgaccga cgagatgcgc caggaagccc gcctgaacgc ggcgcgcctg
     1261 cagacggtcg aggattccct gtccgcccag gaggcctggc tgacgcacgt acggaatctc
     1321 gtcctcgggc gcatcgagcc gggcgagggt gccggcatgc ccgacctggg ccgcggcacg
     1381 ccgtttacgg gcgatctggc ggacatgacg gccgaacccc tctccaacga ctggcaggat
     1441 caccagcaac cggccctgcc ggtggccagg ctgcccgccg attccatggc ccggctcatt
     1501 tcggtcgcga ccgcgggcga tcgcttcctc tcgtcgctga gtttcccgat gcttcccccc
     1561 gtgtccggac ttttgacccg gagcttcgac gcccggggcg ggcactacgc gatcgatctc
     1621 gcggtgaagg agggaacgat cgtgcgcgca gtgggtgacg gctacgtatt cctcgccgac
     1681 tggacgcacg acggcgggca cgtgatcgcc gtccagcacg ccgagggctt cgtgtcggtg
     1741 tacaagcaca accagcagct gctcaaacgt gcgggggacc gggttcagaa ccgggagccc
     1801 atctcgctct cgggcaacac gggcgagatc accacggggc cgcatgtcca cttcgagctc
     1861 tggctggacg ggctcgcgca ggacccggcc gactacctga tcggtttgta actttcctcc
     1921 gctgcctcca ccaacgggat gacagaacca tggcacgttt accgatggaa accgacaccg
     1981 acgccatcaa cctgatcggc gaaggatccg aggtcgaagg cgttctccgc tcgagtaccg
     2041 acgtccgcgt gaacgggcgg gtcaagggtg aggtcaacgt cgagggccgc gtgatcgtgg
     2101 ccgacaaggg cgtgatcgac ggaaccctga tcgcggcgaa cgccgacgtc gcgggcaccg
     2161 tgctcggcga cctgcaggtg gccgagcggc tgctgctgaa gtcgacggct cgcgtggagg
     2221 gcaccatcaa gacggcgcgc ctcgtggtgg aggagggcgc gatgttcgat gggcagtgcg
     2281 acatgggaag gctcgacagg aagcgcaagg atcgcatcga gaaggcgacg gccgaggcgg
     2341 gtccgaagcc ggcggccgat accggcgcca gaccggtggg ggaggcaggg agcaagccgc
     2401 cgagcccgac gaaatcggag ttcatgtttg accccggaga gcggaaagcg ggctgaacca
     2461 cgtcccggct ggcaggcggg actgcgtgcg gccggaccct acatgggtct cggcctccag
     2521 ctcgcgggca gcgtcctctt ctacgtggtg gccggctacc tggcggaccg ctggctcggc
     2581 accgagccct ggctcctcgt cgcgggcgcg gtgatcggca tgatcagttt cttcgtgctg
     2641 ctcgcgcgcg tcgtacggcg cctgaacaag gagacctccc gacgcgcggc ggagcgccgg
     2701 cagaaggccg gaaaagaggc acggaactga gcccgcaggg cggccccgag gggccgcggc
     2761 ggcggggatc cattcattca taattcaacc cctcccgacg atccccccgg gcctttaggc
     2821 gtagtttggc cgtccccgaa aaacggcggt gccccgctac cggatcgcca cggcgcatga
     2881 ccgacggaac ctggatgagt gcccttccgg gtgcggctgc gggcatcgcc tacggaatca
     2941 ctagctggtg gatggttcga cgggccgtcc gccgctcgcc caaaaccttc ctgctactcg
     3001 tcatgggcgg catgctgctc cggctgcttg ccgcctcgat cattgttgtg gcggtcctcg
     3061 tctggctgcc ggtccatccc gcgatcttca tcggtacctt cttcggattc ttcgtcatcg
     3121 ctctcgtcgc cgaggtcatc accctccacc gccagcaacg ggccgccggt ccgcccacga
     3181 cctgatccgc ccgccgcacc ccacacccat gccgacccgc ctcgcattcc gtcttctcgc
     3241 gctcgccgga gcgctggccc tcgtggcggc cagccccgcg gccgcgcagg accatgccga
     3301 ggcggaggaa ctcgacccgg tgcaccacca ggtcgacggc cactacctgg acttcgagcc
     3361 gatcggcaag gtggagctcc cgcggatctt cgtcgtgcga cgggcggacg gctcgctggc
     3421 gctcgacctg ttcggttcgt ctaccgcggc cgtcgagtcg gggcgctaca ccgcgcactt
     3481 cgaggagcac ggtgaggccg ccgcggggga ggccgtggat gccgcggccg ggcacgccgc
     3541 cgggcagcgt gcggcgccgc aggcgacggc tcccgacaag gtgtacgagg ccaccctcgt
     3601 gcccccggac ggcggggaga tcgtgctcga cctctcgtgg acgaagcacc tcgtgttcgc
     3661 gtggatcggt gcgctcatcg tgctcctgct cttcgtccgc gtcggcagcc ggtaccgggc
     3721 gggcatcgga cgggacaccg agccgcgcgg cctgctccag aacctgctcg agaccatggt
     3781 gatcttcgtc cgcgacgaga tcgcccgccc gaacctgggt ccgaagaccg accggtacct
     3841 gccgtacctg ctgacggcct tcttcttcat cctgacctgc aacctcctgg ggctggtgcc
     3901 cttcggggcg acggccacgt cgaacatcat ggtcacggcc gtgctggcca ccttcacctt
     3961 cgtgctcacg cagctcgggg ggtcgaagga ctactggaag cacatcctgt ggccccccgg
     4021 cgtgccgacc ttcgtgaagc ccatcctgat ccctgtcgag atcctgggcc tgttcacgaa
     4081 gccgttcgcg ctcgccatcc ggctcttcgc caacatgacg gcgggccacc tggtgatcct
     4141 gagcctcatc gggctcatct tcgccttcaa caacctgttc ggcccgtcgg ccggctacgg
     4201 cgtggcgccg gtatcggtcg ccttctcgct cttcatctac ctgctcgaga tcctggtggc
     4261 gttcatccag gcgtacgtgt tcacgatgct gtcggcgctg ttcatcggga tggccgtcga
     4321 ggagcacgcc caccacgacg acgggcacgg acacgacgag gtgcacgccc tgcccgaccc
     4381 ctcgcgcgag accatccaca tcgacatccc ggaacgccag cccgtcgcgg ccggctgacc
     4441 cggaccaacg aacccgtttt caccgaccaa ccgatcaaca aatcatcatg gaacctactg
     4501 ctctggcata cctgggtgca ggcatcggcg ccggcctcgt ggccatcggc gccgccctcg
     4561 gcatcggccg tctcgcggga ccggcgatgg agggctccgc gcgtcagccc gaggctgccg
     4621 gcgacatccg gacgtcgatg atcatcgcgg cggccctcat cgagggcgtg gcgctgttcg
     4681 gactcgtcat ctgcatcatc ctggcattca agtaaaagga cgcggccggt gcgagcgttt
     4741 tttcgcgcgt tccggtccaa gtccacgcaa cgtgcccatg gaaccgatcc tggcagccaa
     4801 cctgctctcg ccgaacttcg gcctggtgat ctggatcgcg atcacgtttt tcgcgctcct
     4861 gcttctcctg cgccggttcg cctggggacc gatcaccagc gcgctcgtcg cgcgcgagga
     4921 ccgcatccgg gagtcgctcg agcaggcgca gaaggcgctg gccgaggcgc gtcagatcca
     4981 ggccgacaac gaccgcgccc gccgcgaggc ggacgccgag gcgcagcggt tgctccggca
     5041 gtcgcgcgag gccgccgagg ccatccgttc ggaggaagtc gagcggacgc gcgagcagat
     5101 ccggcagatg cagacctcgg ctcaggccga gatcgcgcgc gagcgggacg gcgccctgga
     5161 gacgctgcgg agcgaggtcg ccgacctggc catccaggca gcggaacgcg tgctggacgc
     5221 gaacctcgac gacgatcgcc agcgcaagct ggtgaacgac ttcctcggcg gcctcccgaa
     5281 gaactgatcc ctgccgaagg gacctcccga accgaatccc atgagccagc tcactatcgc
     5341 gcgacgctac gcccgggccc tgctcgagca ggccgaggcg gagggcgtca cggacccggt
     5401 ggacgccgac gtccgcgcga tccgcgacgc cgtcgcggca tcgcgggagc tcgcggcctt
     5461 tctcgagagc ccgatccttt cgcgcgagcg gaagcagtcg gtcgtgaggg ccctgttcgc
     5521 gggccgcgtc gaggacgtga ccctgcggtt cctcgagatg ctcgcggaca agcggcgcga
     5581 ggggctcttc gcgatgatcg ccgacgccta cctggagcag cgcgacgagc agctcggggt
     5641 ggtcggggtc tcggtgcgga cggcccgcac gctggatgcc gcggccgaga aggcgatcgc
     5701 ccggcggatc ggggagatca cggggaagga gtcccggctc gatgtcgcgg tcgacgcatc
     5761 gctgatggga ggcctcgtgg tccgcgtcgg cgacacggtg tacgacggca gcgtgctgaa
     5821 ccgcctggaa agtctccggg aacggctcat tcatggcacg gcggcgaacg catgatcgcc
     5881 tccgttacgg gcacaggaac cagaaagaca atcgatggca acggcaatac gacccgatga
     5941 ggtcacggca gttctgaagc gggagctcgg cggcttcacg gcggagaccg aggtctacga
     6001 ggtcggcacc gtcctgcagg tcggtgacgg catcgcgcgc atctacggac tgtccaaggt
     6061 ccaggcgggc gagctgctcg attttccctc gagcgggatc accggcatgg tgctgaacct
     6121 ggaagaggac aacgtgggcg ccgtgctctt cggcgatgtc gacgcggtga gggagggcga
     6181 cgaggtccgc cgcacgcagc gcatcgcctc gatccaggtg tccgagagca tgctcggccg
     6241 tgtcatcgac ccgctcggca acccggtcga cgggcgcggg gcgctggccg gtccccggta
     6301 cgagatgccg ctcgagcgca aggccccgag cgtgatttac cgcgagccgg tcaaggagcc
     6361 gctgcagacg ggcatcaagg ccatcgactc gatgatcccg atcggccgcg gccagcgcga
     6421 actggtgatt ggcgaccgcc agacgggaaa gaccgccgtc ctgatcgaca cgatcatcaa
     6481 ccagaagtcc acgcaccaga ccgacaagcc cgtctactgc atctacgtgg cgatcggtca
     6541 gaagaactcg accgtggccc aggtggcgcg ctcgctggag gagcatggcg ccatggaata
     6601 cacggtgatc gtgaacgccg gggcatccac gcccgcgccc ctgcagtaca tcgcgccata
     6661 cgcaggcgcg tgcatcgggg agtatttccg cgacacgggc cgtcacgcgc tggtgatcta
     6721 cgacgacctg tcgaagcagg cggtggccta ccgcgaggtg tcgctgctgc ttcgccggcc
     6781 cccgggccgc gaggcgtacc cgggcgatgt cttctacctg cacagccgcc ttctcgaacg
     6841 ggccgccaag atcaacagca ccgacgcggt cgcgcggcag atgaacgacg ttcccgagtc
     6901 catccgcgag aaggtgaagg gcggcggctc gctgacggcg ctgccggtca tcgagacgca
     6961 ggccggcgac gtgtcggcct acatcccgac gaacgtcatt tcgatcaccg acggccagat
     7021 cttcctcgag tccgggctct tcaacgccgg cgtccggccc gccatcaacg taggcatctc
     7081 ggtgtcgcgc gttggcggca acgcgcaggt caagggcatg aagaaggtgg ccggcacgct
     7141 gcgcctggac ctcgcgcagt tccgcgagct ggaggcgttc tccaaattcg gctcggacct
     7201 tgaccccgcc acgcagcgcc agctgcgccg cggtgagcgc ctggtcgagg tgctcaagca
     7261 gggccagtac gcaccggtgc cggtcgagga gcagatcgcc atcatccacg tcgccacgca
     7321 ggggctgctc gacgccatcc cggtgaaccg ggtgaaggag ttcgagcgcg agttcatcga
     7381 caagctgcgc ctgcggcacc agggcatcct cgacggcatc cgcgacaccg gtgccctctc
     7441 ggacgacgcc gccgcgaccc tgaagcaggc ggccggggag ctggtccgag cgtacaccgc
     7501 ctgattctgc cccgagcccg aaaccgtctc ccgacatggc cagtctccgc gacatccgga
     7561 accgcatcgc ctcggtccag aacacgcagc aggtgacccg cgccatgaag atggtggcgg
     7621 cggccaagct gcgccgggcc caggaagcga tcttccggac gcggccgtac gcctacaagg
     7681 tcggcgagat catcagccac ctgaagcggc acaccgaccc gggatcgcac ccgatgttca
     7741 tcgctcgggg ggagacgcgc gccgtactgc tcgtcgtggt gacggcggac cgcgggctcg
     7801 ccggggcgtt caacacgaac atcatcaagg ccgccgagca ggcgatccac gagcggttcg
     7861 cggcgctgca cgcgtcgggc aacctgtacc tcctgtgcgt cggccggaag gggcacgagc
     7921 atttccagaa gcgcggcttc cgcctggtgg gcgatttccg cttcgtgttc gatcacctgg
     7981 aattcgggac ggcgaaccgg atcgtgcacg aagcgctcga cggcttcacc ggcggtcgct
     8041 gggacgaggt ccatctcgtg tacaacgagt tcaagaacac gatctcccag aaccgcatcg
     8101 tcgagccgtt tctcccgatc cgcgcggagc agttcctcac cccggtcatg gagaaggaac
     8161 tcgatcacca ggaagaagag aaggtggggg tcgcggtaga ctacatcttc gagccccgcg
     8221 cctccacgat cctcgaggcc ctcgtccctc ggtacctgaa ctatgaagtc tggcgggcgc
     8281 tgctcgagtc gaacgcggcc gagcagggcg cccgcatggt ggcgatggac aacgccacgt
     8341 cgaacgcctc cgagctgatc cgcgacctga agctcaagta caaccgggcc cggcaggacg
     8401 cgatcaccaa ggaactgatc gagatcacgt ccggcgcgga cgcgttggca ggaggctgac
     8461 ggcaggtccc cgaccggccg atgcacgcga tccccgatgg aattcctctc ggacatcgac
     8521 cagaagctcg tcgggctgtt cgaggagtac gggctctggg tctacgtcgt tctcttcgcg
     8581 atcgccttcc tcgagtcgac ggtcacgccg ttcctgccgg gtgacgccct ggtgttcgcg
     8641 gccggcgccg tcgccgcggc cggcgcgacg acgcttcccc tcatgtgggt gctgttcgtc
     8701 ggcgcgacgg tcctgggcac gatgtcgagc tacgaactgg gccggcgggt gggcccgagc
     8761 gcgttccgcg agcgccggcg cttcctgaac acggagcagc tcgagcacgc tcgccggttc
     8821 ttcgaccggt acggagggcg cacgctggcc gtcggccggt tcataccggt ggtccggacg
     8881 ctcgcgcccg tcgtggccgg catactgcgc atgccgtacg cgcgcttcat gggatacacg
     8941 gtgctcggct gcgtactctg ggtcacgctg atgctcgtgt cgggctactt cgtggatacc
     9001 gtccccgtcc tgagggcgcg gttcggtatc gtgctcgcgg ccgtcttcct gctctcgatg
     9061 gtgccgccga cgatcgaatg gctgtgggac cacatcgggc ggaggatcta tcgcgcggtg
     9121 ctccgccggt tcgggcacga agcgtgagcg ttcggggggc ggccgcgggc cggcgcgtcc
     9181 gccgccccat gcggagtacg atcgggcgcc tgattcgtgc gtcagtcgcc ccgctcgaca
     9241 gacgccggca cttggaaacc tggcccgccg tgtgcagttt ctgcctcccc ggtcgcacac
     9301 ggaccacccg gagaggtggg tgagcggctg aaaccaccgg tttgctaaac cggcgtacgt
     9361 cgcaaggcgt accgagggtt cgaatccctc cctctccgca cgaacgcccg cctgcgcccg
     9421 cgcagcgggc gttcggcgtt tcggggaatc cccgttcgag cttgctcgat aaggggaggc
     9481 cccgaaacgc cgaatcccca ccggggataa caggcggacg tccgtgcagg acctcccccc
     9541 gcgggatacc cagcgagcaa agcgagcgga aacccgcccg
//
LOCUS       DBNHQZ010000268         6569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2923698, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000268 DBNHQZ010000000
VERSION     DBNHQZ010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2923698"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="AC16YU_07270"
     CDS             complement(<1..280)
                     /locus_tag="AC16YU_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065061.1"
                     /translation="MPVTGPIKLSDGTAVVIRPVRPDDRPHFEQGLRELSARSLFHRF
                     HGTFRPTSKDWDYLTRVDQHDHLAVCAIQVSAAEDHGAGVARCVRRSPD"
     gene            complement(354..851)
                     /locus_tag="AC16YU_07275"
     CDS             complement(354..851)
                     /locus_tag="AC16YU_07275"
                     /inference="COORDINATES: protein motif:HMM:NF020496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR6065062.1"
                     /translation="MTPLEFVFAAPVLRQGDGLRFHYLPLPIEVADELGREGVRRVVV
                     ELNGVPLRRAVMQSSDFGPHLVTGLPILREIGATLGDMVTVVLTPDPDPDAIDMPEEL
                     EAALAQDPEAARRFAAFTPGMQRSLAYYVTQAKRTETRVKRSLELAHKLRTHSLHGDS
                     RPPGG"
     gene            complement(859..1320)
                     /locus_tag="AC16YU_07280"
     CDS             complement(859..1320)
                     /locus_tag="AC16YU_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065063.1"
                     /translation="MHRRTPLFLVAILAVSAFSAPSARAQSGGTWLGFELGSPTGIVI
                     MFDRGAQPDLELLAAWAIGHRFFLNGHMLWLANESADPPLSLKYGPGVFVGFRDRDPG
                     SDDMTVGVSFRLEGTYHWDQFQVYLALTPRLEIIERTEGFMGGGLGFRMRL"
     gene            complement(1410..3287)
                     /locus_tag="AC16YU_07285"
     CDS             complement(1410..3287)
                     /locus_tag="AC16YU_07285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR6065064.1"
                     /translation="MPVLRRLPLAFVLLALTALPALAQTGVWERLQEVAVVDQKVMMP
                     MRDGVRLATDIFRPKTDEPVPIVFSRTPYNFNPWGDGELRERAFSTALRYVEAGYAYV
                     VQNERGRYFSEGEWDILGPPTTNGYDALSWMAAQPWSNGKVGLIGCSSTAEWQMEVAS
                     MNHPALAAIIPQGFGAGVGRVGRFYEQGNWYRGGAQQMLFTAWLYGVQDIQRPTFSPE
                     LTQEDLVRVSRFYDLAAEKPPVDWSVALRHLPVIDIMKAVDGPKGIYEDMMARKPNDP
                     AWYEGGLYHDDDPINVPGFWFVSWYDVSQGPNLALYNHVRDTAPDPAVRDAQYLVIAP
                     TTHCGFTRATASTVVGERPVGDARLDYDALTTGWFDLWLKGDRNGFTDETPRVQFYTM
                     GSNRWQSSDTWPPSDAVPMTLFLASDGAANSVFGDGRLSTEPPAADSPDRFSYDPAFP
                     VPSLGGQVCCTGSAVEGGSFDQRQMEARNDILVYTSEPFANGIEVTGTIEITLFVSSD
                     ARDTDFTVKLVDVYPDGRAFNIDETILRARYREGFDREVFLEDGEIVELGLSPMSTSN
                     FFGPGHRIRVEVSSSNFPRFERNLNTGGNNYDETEGVVAHNAVHHSREHPSRIVLPVR
                     R"
     gene            3486..4166
                     /locus_tag="AC16YU_07290"
     CDS             3486..4166
                     /locus_tag="AC16YU_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HIR6065065.1"
                     /translation="MPTSNPIARLLSRNPFGSIQEHMRAVVACADEVPLLFEAVQADD
                     AERIRAVAVTIDDKEAEADRIKNQLRLHLPRTMFLPVNRRDLLDVLDMQDSIADVAQD
                     IADLVVQRSMRTPEPLREDLVPFVRSCVDVCHAALRIVEELDELLETGFRGREATAVD
                     EMIRSLNEAEDRTDVTGNQLASRLFEYEDRMPPVSAIFWYRLLDLIGDLADYAEKVGN
                     RLRLMIAQ"
     gene            4179..5444
                     /locus_tag="AC16YU_07295"
     CDS             4179..5444
                     /locus_tag="AC16YU_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6065066.1"
                     /translation="MDVIAAYGPWFVGLAVVAGLYMTWGIGANDAANAMGTSVGSKAI
                     TVRQAIAISAVFEFSGAFLAGGRVTSTIRKGIIDPSGIVGSPEILVFGMLASLLAAGT
                     WLWVASSRGWPVSTTHSIVGAIIGFAVVGIDLGSVIWSKVGEIVASWIVSPVLGGIVA
                     FFVMLSIRRLILDTDVPFEKARRWGPVYVFLVGFLISLVTLFKGLTHLDIRLTGLESV
                     AVSLGVGFLVMGAGWLFIRRVRVDPDADRTFHFASVERVFAPMMVFTACSMAFAHGSN
                     DVANGIGPLAAVVSIVASGGEVMQSADLPVWILALGGGGIVVGIATMGYRVMRTIGTK
                     ITELTPTRGFSAELAAAATVVLASRTGLPVSTTHILVGAVLGVGLARGIGAIDLRVVG
                     GILTSWLVTLPVAALLSATFFFALRLVFG"
     gene            complement(5458..>6569)
                     /locus_tag="AC16YU_07300"
     CDS             complement(5458..>6569)
                     /locus_tag="AC16YU_07300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065067.1"
                     /translation="LSIDDEIHSVFPELPDFGHPITIRQMLNHTSGLRSLHDVLAIAG
                     WRGTDRRTNEDIWRVMLRQADLNFEPGSEYLYCNTGYMLAAMIVERVTGERFADWMAS
                     QVFAPLGMTRTSVQDDYDRVYRDTASSYSPVDSTGFAPSVPFWAYYGSGNVHTTAADL
                     ARWAHNFTTGGLGGAAVIARMQERGVLTSGDTLSYALGIEVTSAGTLRMLTHGGSIGG
                     YRASLMIFPEIDAGVVLLGNGSALPARAMLPTAADHLEEVRRALESAGEEERRGGPPP
                     TESEAESWSPGLDELASLTGRYYSPELDTAYDVRLENGALVAWHMRHGTIALEPEAPG
                     RFDSRLARFEFGARDGAPGVFLSNGRARNVWFRRE"
BASE COUNT          993 a         2197 c         2322 g         1057 t
ORIGIN      
        1 cgtccgggct tcgccggacg cagcgggcca cgcccgcgcc gtggtcttcc gcggcggaga
       61 cctggatggc acagaccgcc aggtggtcgt gctggtccac ccgcgtcagg tagtcccagt
      121 ccttggacgt gggtcggaac gtgccgtgga atcggtggaa gagcgagcgg gccgaaagct
      181 cgcgcagtcc ctgctcgaag tgcggccggt cgtccggtcg caccggccgg atgacgacgg
      241 cggtgccgtc ggagagcttg atgggacccg tgaccggcat gcccctgtaa cggtgccgct
      301 cgccttttgc cccggccggg ccgggaagac tccgtcaatt gggccggggt tcgtcagccg
      361 ccgggcggcc gcgaatcgcc gtggagcgag tgggtccgca gcttgtgcgc cagctccagg
      421 gaccgcttca cgcgcgtctc cgtgcgcttt gcctgcgtga cgtagtacgc gagcgaccgc
      481 tgcatcccgg gcgtgaacgc ggcgaagcga cgggccgcct cgggatcctg cgcgagggcg
      541 gcctcgagtt cctccggcat gtcgatggcg tccggatcgg gatccggcgt gagcacgacg
      601 gtgaccatgt cgccgagcgt ggcaccgatc tcgcggagga tcggcagccc cgtcacgagg
      661 tgcggcccga agtcggagga ctgcatgacg gcccggcgca gcggcacgcc gttcagttcg
      721 acgacgaccc ggcggacgcc ttcgcggccg agctcgtcgg ccacctcgat cgggagcggc
      781 aggtagtgaa agcgaagccc gtccccctgt cggaggacgg gcgccgcgaa tacgaactcc
      841 aacggggtca tggccgggtc agagccgcat ccggaacccc aggccgccgc ccatgaagcc
      901 ctccgtcctc tcgatgatct ccaggcgggg ggtgagggcg aggtagacct ggaactggtc
      961 ccagtggtag gtgccctcga ggcggaagct gacgcccacg gtcatgtcgt ccgagccggg
     1021 gtcccgatca cggaagccca cgaacacgcc cggaccgtac ttgagcgaga gcggagggtc
     1081 ggcactctcg ttggcgagcc agagcatgtg gccgttcagg aagaagcggt ggccgatcgc
     1141 ccaggccgcg aggagctcca ggtcgggctg ggcgccgcgg tcgaacatga tcacgatgcc
     1201 ggtgggcgat ccgagctcga agccgagcca tgttccaccc gactgggccc gggcggaagg
     1261 ggccgaaaac gccgaaacgg cgagaatcgc caccaggaag agaggggttc tgcgatgcat
     1321 acgcgtcctc agagtcggtt cgtgaagcgt acacgtcaaa cgcgcaagcc ccctatgtgg
     1381 tcctcaacgg cgaacggcca atccgcccgt cagcggcgca cggggagcac gatccgggac
     1441 ggatgttcgc gcgagtgatg cacggcattg tgggcgacga cgccctccgt ttcgtcgtaa
     1501 ttgttgcccc ccgtgttcag gttgcgctcg aagcgcggga agttcgagga cgacacctcg
     1561 acccggatgc ggtggccggg gccgaagaag ttgctcgtgg acatcggact caggcccagc
     1621 tcgacgattt cgccgtcctc caggaacacc tcgcgatcga agccctcgcg gtagcgcgcg
     1681 cgcaggattg tctcgtcgat gttgaaggcg cgcccgtcgg gatacacgtc cacgagcttc
     1741 accgtgaagt ccgtgtcacg ggcgtccgac gagacgaaga gcgtgatctc gatggtgccg
     1801 gtcacctcga tgccgttcgc gaacggctcg ctcgtgtaca cgaggatgtc gttacgggcc
     1861 tccatctggc gctggtcgaa ggacccgccc tcgaccgcgc tgcccgtgca gcagacctgt
     1921 cctccgagcg acggcaccgg gaaggccggg tcgtacgaga accggtcggg gctgtccgcc
     1981 gccggcggtt cggtcgacag gcgtccgtcc ccgaagaccg agttcgccgc gccgtccgag
     2041 gcgaggaaga gcgtcatggg gaccgcgtcc gagggcggcc aggtgtcgga cgactgccac
     2101 cggttcgagc ccatcgtgta gaactggacc cgcggcgtct cgtccgtgaa gccgttccgg
     2161 tcgcccttca gccacaggtc gaaccatccc gtggtgagcg catcgtagtc caggcgggca
     2221 tcgccgaccg gcctctcgcc gaccaccgtg ctcgccgtgg cccgcgtgaa cccgcagtgc
     2281 gtcgtcggcg cgatcaccag gtactgcgca tcgcgcacgg ccgggtcggg cgcggtgtcg
     2341 cgcacgtggt tgtagagcgc gaggttcggt ccctgggaga cgtcgtacca cgacacgaac
     2401 cagaatccgg gcacgttgat cgggtcgtcg tcgtggtaga gaccgccctc gtaccaggcc
     2461 ggatcgttcg gcttgcgggc catcatgtcc tcgtagatcc ccttcggccc gtcgaccgcc
     2521 ttcatgatgt cgatgaccgg gaggtggcgc agcgcgacgg accagtccac cggcggcttc
     2581 tcggccgcga ggtcgtagaa gcgcgagacg cggacgaggt cctcctgggt cagttccgga
     2641 gaaaacgtag gccgctggat gtcctgcacg ccgtagagcc acgccgtgaa gagcatctgc
     2701 tgcgcgccgc cccggtacca gttgccctgc tcgtagaagc gcccgacgcg gcccacgccg
     2761 gcaccgaatc cctgcggaat gatcgcggcg agcgccgggt ggttcatgga cgcaacctcc
     2821 atctgccact cggccgtcga cgagcacccg atgaggccca ccttgccgtt ggaccacggc
     2881 tgggcggcca tccaggacag ggcgtcgtag ccgttggtcg tcggggggcc gaggatgtcc
     2941 cactcgccct cggagaagta gcggccccgc tcgttctgca ccacgtaggc gtagccggcc
     3001 tccacgtagc ggagcgccgt cgagaaggcg cgctcccgga gttccccgtc gccccacgga
     3061 ttgaagttgt agggcgtgcg cgagaacacg atcggcacgg gttcgtcggt cttcgggcgg
     3121 aagatgtcgg tcgcgaggcg gactccgtcg cgcatcggca tcatgacctt ctggtcgacg
     3181 acggcgactt cctggaggcg ctcccacacc ccggtctgcg cgagcgcggg aagggcggtg
     3241 agggcgagga ggacgaaggc gagcgggagg cggcgaagga cgggcatggc ggcggcggtt
     3301 gaatgcgcaa tccctttagg tccgcaactc gcccggcatc ttctgccggg agggagcagg
     3361 atctcatgta ggggaatccc cttcgtcgct cggcgggcgt cctgccccgc gatttcacat
     3421 tgtgaccgaa tcgttaccgg tacattacgc ggtgccatcc accagccgcc gcaaggcccc
     3481 gcgacgtgcc cacctccaac ccgattgccc ggctcctgtc gcggaacccg ttcgggtcca
     3541 tccaggagca catgcgcgcc gtcgtcgcgt gcgccgacga ggtgccgctg ctgttcgagg
     3601 cggtccaggc cgacgacgcg gagcggatcc gcgcggttgc cgtcacgatc gacgacaagg
     3661 aggcggaggc cgaccgcatc aagaaccagc tccgcctgca ccttcccagg accatgttcc
     3721 tgccggtgaa ccggcgtgac cttctggacg tcctggacat gcaggactcg atcgccgacg
     3781 tcgcccagga catcgccgat ctcgtggttc agcggtccat gcgcacgccg gagccgctgc
     3841 gcgaggatct cgtgcccttc gtccgctcgt gcgtggacgt atgccacgcg gcgctgcgca
     3901 tcgtggaaga gctcgacgaa ctgctcgaga ccggcttccg cggccgcgag gccacggcgg
     3961 tcgacgagat gatccggagc ctgaacgagg ccgaggaccg gacggacgtg accggcaacc
     4021 agctcgcgag ccgtctcttc gagtacgagg accggatgcc gccggtgtcc gccatcttct
     4081 ggtaccggct gctcgacctg atcggggacc tggccgacta cgccgagaag gtggggaacc
     4141 ggcttcgcct gatgatcgcg cagtaagggc ggccgacaat ggacgtcatc gcagcgtacg
     4201 gtccgtggtt cgttgggctc gccgtcgtcg cgggcctgta catgacgtgg ggcatcgggg
     4261 cgaacgacgc cgcgaacgcc atgggcacgt cggtgggctc gaaggccatc accgtccggc
     4321 aggccatcgc gatctcggcg gtattcgagt tctcgggcgc gttcctggcc ggcgggcgcg
     4381 tgaccagcac catccggaaa ggcatcatcg atccgtccgg cattgtcggc agcccggaga
     4441 tcctggtgtt cgggatgctg gcctcgcttc tcgcggccgg cacctggctg tgggtcgcct
     4501 cgtcgagggg gtggcccgtc tcgaccaccc actcgatcgt cggcgcgatc atcggcttcg
     4561 ccgtcgtcgg catcgacctc ggctcggtga tctggtccaa ggtgggagaa atcgtcgcga
     4621 gctggatcgt gtcgcccgtc ctgggcggca tcgtggcgtt cttcgtgatg ctcagcatcc
     4681 gccggctgat cctggacacg gacgtgcctt tcgagaaggc ccggcgctgg ggcccggtct
     4741 acgtgttcct ggtcgggttc ctgatcagcc tcgtgaccct attcaagggg ctcacccacc
     4801 tggacatccg cctgacgggc ctcgagagcg tcgccgtctc gctgggcgtg ggattccttg
     4861 tgatgggcgc cggctggctc ttcatccgca gggtgcgcgt ggacccggat gccgaccgga
     4921 ccttccactt cgcgagcgtc gagcgggttt ttgcgccgat gatggtattc acggcctgct
     4981 cgatggcctt cgcccacggt tcgaacgacg tggcgaacgg catcggcccg ctggccgcgg
     5041 tcgtgagcat cgtcgcctcg ggcggggagg tgatgcagag cgccgacctg ccggtctgga
     5101 tactggccct cggcggcgga ggcatcgtgg tcggcatcgc gaccatgggc taccgcgtca
     5161 tgcgcacgat cggcacgaag atcaccgagc tcacaccgac gcgggggttt tcggccgagc
     5221 tcgccgccgc ggcgaccgtg gtcctggcct cgcggacggg tcttccggtg tcgacgaccc
     5281 acatcctggt cggcgcggtc ctgggcgtgg ggctcgcgcg cggaatcggg gcgatcgacc
     5341 tgcgcgtcgt gggcggcatt ctcacctcct ggctcgtcac gctgccggtt gcggccctgc
     5401 tgtcggccac gttcttcttc gcgctgcgcc tggtcttcgg gtgaccggag agcgggctca
     5461 ctcccgccgg aaccacacgt tgcgggcgcg accattggac aggaacaccc ccggcgcccc
     5521 gtccctcgcc ccgaactcga agcgggcgag ccgcgagtcg aagcgcccgg gggcttccgg
     5581 ttcgagcgcg atcgtgccgt gccgcatgtg ccacgccacc agtgcgccgt tctcgaggcg
     5641 gacgtcgtag gccgtgtcga gctcggggct gtagtatcgc cccgtgagag aggccagttc
     5701 gtccaggccc ggggaccagg attcggcttc ggattcggtc ggtggcggcc cgccgcgccg
     5761 ctcctcctcc ccggcgctct cgagggcccg gcgcacctcc tccaggtgat cggcggccgt
     5821 cggaagcatc gcacgcgcgg gcagggcgga gccgttgccg agcagcacca cgccggcgtc
     5881 gatctccggg aagatcatga ggctcgcccg gtagcccccg atcgacccgc cgtgcgtgag
     5941 catgcggagc gttccggcgg aggtcacctc gattccgagc gcgtacgaga gcgtgtcgcc
     6001 cgaggtcagc acgccgcgct cctgcatccg ggcgatgacc gcggctcccc cgagcccgcc
     6061 cgtcgtgaag ttgtgcgccc agcgggcgag gtccgcggcg gtcgtatgga cgttgcccga
     6121 tccgtagtac gcccagaagg gtacgctcgg cgcgaagccg gtcgaatcca ccggggaata
     6181 cgaggacgcc gtgtcgcggt atacacggtc gtagtcgtcc tggaccgagg tacgggtcat
     6241 gccgagcgga gcgaacacct gcgaagccat ccagtcggcg aaccgctcgc cggtcacgcg
     6301 ctcgacgatc atcgccgcga gcatgtagcc ggtattgcag tacaggtact cggaccccgg
     6361 ctcgaaattg agatccgcct ggcggagcat gacgcgccag atgtcctcgt tcgtgcggcg
     6421 atccgtcccg cgccagccgg cgatcgcgag cacgtcgtgc aggctccgca gcccactcgt
     6481 gtggttgagc atctgccgga tcgtgatcgg gtgcccgaag tccggcagct cggggaagac
     6541 cgagtggatc tcgtcgtcga tcgacagga
//
LOCUS       DBNHQZ010000269         2895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1163563, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000269 DBNHQZ010000000
VERSION     DBNHQZ010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1163563"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="AC16YU_07305"
     CDS             complement(<1..1249)
                     /locus_tag="AC16YU_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR6065068.1"
                     /translation="MADSGAKRVLGAGLLGLALGAAACAVPVPPSGGPADTTPPSLVA
                     SEPAGGTVRFSGDRVHLSFDEYVDERSVASSLTVTPAPARPPEVHFGGRSVEIRFREP
                     LRDSTTYILALDSGFRDAHGVALRTPIQIAFSTGDVIDAGRLSGRVLEAETGRPAASA
                     NVFAYAPDPGTPPDSLPEEPVYRTQTDGEGRFRLDHLAERPFLVVAFRDANRNRRLDP
                     GEPLATPPVPWSTADSAGTEPDRPWILASRDAGPPVLRRTVARSSTRLELLFTEGVRL
                     ANRGTEGWSVVDSSGARHVRVRLADTYPDDSRTLVLRTEPMSTGWWEVRGDSAVSDSA
                     GSVVARLRASFAASAASDTALARFRGFGGDTLGGGPAGVRVLAPWAAARVSWSDPVHA
                     DTLRSAIAVRDTSGLAIAFSLHPV"
     gene            complement(1239..1826)
                     /locus_tag="AC16YU_07310"
     CDS             complement(1239..1826)
                     /locus_tag="AC16YU_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065069.1"
                     /translation="MIRLPIALVLVAAVPARDAGAQATNAERMGALAVRCVAERTAGA
                     DTVSVTGPERLPFIRSAVVAAWQASGVTVYEGSEGAPGTAVRLEVEDAGVRYDRADDG
                     MIARSVHLELMVRVTAQGGLILADDTCRGSESDLVERRAIESLGSPAWPETLAEEPAH
                     GWLRRILQPVVVTAATAAGVFLFFSLRSRRADDGG"
     gene            complement(1891..>2895)
                     /locus_tag="AC16YU_07315"
     CDS             complement(1891..>2895)
                     /locus_tag="AC16YU_07315"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6065070.1"
                     /translation="VKALAAWMTWKCAVVNVPFGGAKGGVVCNPREMSPGELERMTRR
                     YTANLIDVFGPDKDIPTPDMNTNEQIMAWILDTYSMHAKRTENAVVTGKPILLGGSRG
                     RREATGRGVMTVTLAAMERLGMRPAQCSVAIQGFGNVGSIAAQLLGEQGCRIVAISDV
                     TGGYYNADGIDVNAAIAYTEGHQRSLEGFPGAEPITNEELLTLDVDVLVPAAKEDQIT
                     RHNAPDIKAKIIAEGANGPTTPRADDILNERGVLVIPDILANAGGVTVSYFEWVQDRQ
                     GYFWTTDRVNRRLDRMMRTAFDSVFDVASKHDVSLRIGAYVYAIDKVAGALRLRGIYA
                     "
BASE COUNT          405 a         1035 c         1064 g          391 t
ORIGIN      
        1 cgacggggtg aagcgagaag gcgatcgcca ggcccgaggt gtcgcggacg gcgatcgcgg
       61 agcggagggt gtcggcgtgg acggggtccg accagctgac gcgggcggcc gcccacggcg
      121 cgagcacgcg caccccggcg ggcccgccgc cgagcgtgtc gccgccaaag ccccggaagc
      181 gggccagggc ggtatccgag gccgcggacg cggcgaacga cgcgcggagc cgcgcgacga
      241 cgcttccggc ggaatccgag acggccgagt ccccccgaac ctcccaccag cccgtggaca
      301 tcggctccgt gcgcaggacc agcgtgcggg aatcgtccgg gtacgtgtcg gcaaggcgca
      361 cgcgcacgtg ccgggcgccg gaggagtcca cgacgctcca gccctccgtt ccgcggttgg
      421 ccaggcggac gccctccgtg aagaggagtt cgagcctcgt cgatgaccgc gcgacggtcc
      481 tccgcaggac cggcgggccc gcatcgcgcg aggccaggat ccagggccgg tcgggttcgg
      541 ttccggccga gtccgccgtc gaccagggca ccggcggcgt ggcgaggggc tctccggggt
      601 ccagccgccg gtttcggttg gcgtcccgga aggcaacgac caggaagggg cgctcggcga
      661 gatgatccag tcggaaccgt ccttcgccgt cggtctgtgt ccggtagacc ggctcctcgg
      721 gcagggaatc gggcggcgtc cccgggtcgg gagcgtaggc gaacacgttg gccgaggccg
      781 cggggcgccc cgtctccgcc tccagcaccc ggccggagag ccgcccggcg tcgatgacgt
      841 ccccggtcga gaatgcgatc tggatcgggg tccggagtgc cacgccgtgg gcgtcgcgga
      901 acccggaatc gagcgcgagg atgtaggtgg tcgagtcgcg aagcggctcc cggaaccgga
      961 tttccacgct ccggcccccg aagtggacct cgggcggccg cgccggggcg ggcgtgaccg
     1021 tcagcgagga cgccacggac cgctcgtcca cgtactcatc gaacgacaga tgcacccggt
     1081 cgcccgagaa gcgcaccgtg ccgcccgccg gctcgctggc cacgagggac ggcggcgtcg
     1141 tgtcggccgg accgccgctc gggggcacgg gcaccgcgca ggcggcggcc ccgagtgcga
     1201 ggcccagcag cccggctccg agaacccgtt tcgcgccgct atccgccatc gtcggcccgg
     1261 cggctgcgca gggagaaaaa gaggaagacg ccggcggccg tcgccgccgt gacgacgacc
     1321 ggctgcagga tgcggcgcag ccatccgtgc gccggctcct cggcgagagt ttccggccag
     1381 gccgggctgc cgagcgactc gatcgcgcgg cgctcgacga gatccgactc ggatccacgg
     1441 cacgtgtcgt ccgccaggat gagcccgccc tgcgccgtga cccgcaccat cagttccagg
     1501 tgcacggacc gggcgatcat gccgtcgtcc gcccgatcgt accggacccc ggcgtcctcc
     1561 acctcgagac gcacggccgt gccgggcgcg ccttcggagc cctcgtacac ggtgacgccg
     1621 gaggcctgcc acgccgccac gacggccgac cggatgaacg ggagccgctc tggccccgtc
     1681 accgagaccg tgtccgcgcc cgcggtccgc tcggcgacgc agcgcacggc cagtgcgccc
     1741 atgcgctccg cattggtcgc ctgcgccccg gcgtcccgcg cggggacggc ggcgacgagg
     1801 acgagcgcga tggggaggcg gatcacggaa cggcggtcgg gttcgggagg acggaggccc
     1861 aacgccccgg cggtcgggac gggtcccgtc tcatgcgtag atgccccgca ggcgcagggc
     1921 tccggccacc ttgtcgatgg cgtagacgta ggcgccgatg cgcagggaga cgtcgtgctt
     1981 cgacgcgacg tcgaacaccg agtcgaaggc ggtccgcatc atgcgatcca ggcggcgatt
     2041 cacgcggtcc gtcgtccaga aataaccctg ccggtcctgc acccactcga agtacgagac
     2101 cgtgacgccg ccggcgttgg ccaggatgtc cgggatgacg agcacgccgc gctcgttcag
     2161 gatgtcgtcc gcgcgcggcg tggtggggcc gttcgcgccc tcggcgatga tcttcgcctt
     2221 gatgtcgggc gcgttgtgcc gggtgatctg gtcttccttc gcagccggga ccaggacgtc
     2281 gacgtccagc gtgagcagct cctcgttcgt tatgggctcg gcaccgggga agccctcgag
     2341 cgatcgctgg tggccctccg tgtacgcgat ggccgcgttc acgtcgatgc cgtcggcgtt
     2401 gtagtagccg ccggtcacgt cgctgatcgc gacgatccgg cagccctgct cgccgagcag
     2461 ctgggcggcg atcgacccga cgttgccgaa gccctggatg gccaccgagc actgcgccgg
     2521 gcgcatcccc aggcgctcca tggcggcgag cgtcacggtc attacgccgc gcccggtcgc
     2581 ctcgcggcgg cctcgcgacc cgccgagcag gatcggcttg cccgtgacga ccgcgttctc
     2641 ggtgcgcttg gcgtgcatcg agtacgtatc cagtatccag gccatgatct gctcgttcgt
     2701 gttcatgtcc ggggtcggaa tgtccttgtc gggcccgaac acgtcgatca ggttggccgt
     2761 gtagcgtcgc gtcattcgct ccagctcgcc cgggctcatc tcgcgcgggt tgcagacgac
     2821 gccacccttg gcccctccga agggcacgtt cacgacggcg cacttccagg tcatccacgc
     2881 ggcgagcgcc ttgac
//
LOCUS       DBNHQZ010000270        11539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3739544, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000270 DBNHQZ010000000
VERSION     DBNHQZ010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3739544"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..3477
                     /gene="rpoC"
                     /locus_tag="AC16YU_07320"
                     /pseudo
     CDS             1..3477
                     /gene="rpoC"
                     /locus_tag="AC16YU_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            3648..3953
                     /locus_tag="AC16YU_07325"
     CDS             3648..3953
                     /locus_tag="AC16YU_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065071.1"
                     /translation="MLRDAYLHLGLGGTILVGGLVMLTAFSWFVAMAGAVTRPMTPLR
                     RGLLLAVLTALPPSAILVFARFVAVTRGEYRTHRKEAAAAGRASELMLYGSRLSAAA"
     gene            complement(3963..4628)
                     /locus_tag="AC16YU_07330"
     CDS             complement(3963..4628)
                     /locus_tag="AC16YU_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR6065072.1"
                     /translation="MSETFVIEPLHPLDELFAMAREAEPPEEPGPFREPDFVRVSDMD
                     PRILMDLRYASSDNFLGTQIYPEEEALLQRPAAEALRLVQDHLEARGLGLVVYDAYRP
                     WWVTWLFWEATPPEYRTFVATPSLGSRHNRGCAVDVGLVDLASGIPLAMPSGYDEFTE
                     RAYADWNGGPADRRANRDLLISTMRGRGFEVYRAEWWHFDFVDWPRYAIENRDFAELV
                     RPG"
     gene            complement(4642..4950)
                     /locus_tag="AC16YU_07335"
     CDS             complement(4642..4950)
                     /locus_tag="AC16YU_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065073.1"
                     /translation="MPDAPRDPDTIRDTVPIRPLRRWASRLLVTAALLAAAALGVFAA
                     REMGEMATDPNVRERFEAVERMRRDAAPTDSTVTDSTATDSTGLPADSAGLPEAILPD
                     "
     gene            complement(5104..6360)
                     /gene="pepT"
                     /locus_tag="AC16YU_07340"
     CDS             complement(5104..6360)
                     /gene="pepT"
                     /locus_tag="AC16YU_07340"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR6065074.1"
                     /translation="MDRFLRYVRIDTQSDPDSTNVPSTEKQKDLGRVIQAELSGIGLD
                     ATIDEFGYVWATLPATASAPDGDPLPVVALLAHMDTSPDAPGAGVRPIVHPPYGGETV
                     ILPGNPSVTLDPARQPALLEHVGENLITSDGTTLLGSDDKAGVAVLVQLAADLVDDSA
                     PRPEVRLCFTVDEEIGRGVDHLDLGSLSARIAYTIDGSDVDTISFETFNAAEATVTVR
                     GVGVHPGYARGVMVNAVRIVGRLIAALPAAESPEHTDGVRGFIHPHTLAAGDVGRASV
                     RLILRDFSDDGLERRKRLVSSLVESLRLEYPAASIDLEIRDQYRNMRSYIEEIDPRAV
                     HFAREAAAGIGITLRAERVRGGTDGSRLSEAGIPTPNLFNGGMDYHSCFEWNTVQSLE
                     RSLRFVRAVVRYWGEHGHESRTGALD"
     gene            complement(6383..7360)
                     /locus_tag="AC16YU_07345"
     CDS             complement(6383..7360)
                     /locus_tag="AC16YU_07345"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR6065075.1"
                     /translation="MALPRPTILHLALGALLASGGCADGTDRFAPIRVEWSETPPPVG
                     SLPDGVRLLAGTSPDLPLRTWAIRVDPTAEGVRIGVATAGDDDGLATPTEMARSAGAC
                     ALINGGFYRIDEAGRGRHIGLLVRDRERISSALEGVVWQQVRYPTVRATIGADSAGIL
                     QIRWAGASDDTIRGFGRPFDNALGRPAIPPDGRSGAAWFPEWALSAGPVLLSDGEFRV
                     TREEEAFFDAYIPRVHPRSAAGVTADGQLLLVVVDGRQQRSRGVRLEELAVILRDLGA
                     VWAMNLDGGGSSALVVNGRLVNRPAGSDREREIVSAITVMCNAEDSRSG"
     gene            7495..8403
                     /locus_tag="AC16YU_07350"
     CDS             7495..8403
                     /locus_tag="AC16YU_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR6065076.1"
                     /translation="MRLAIRSSILATLLSAWVLPACVLAGPQPSVDTIKRSFQVSPGG
                     TLQVDIDFGNVIVESSGDGMVYVELQRTVGSENEDEAKRLLARHEWEIDQVGNDVVVE
                     SRYEFEGTDRWINRNRRDNVFRLKVVVRIPDEYSVDFTTGAGNVSITDVGGWVEGTTG
                     AGNITIGEVTGSVEIRSGSGNIDIAGIDGSIEVTSGAGNVTLGYVTGEIQANTGAGNI
                     EARITRQPGGDSSVETGAGNVTVYLGSDVGMQVDARASVGSAECDFGLKVSGKWMSKS
                     FEGDVNGGGPALTMRSGVGNVALKRI"
     gene            8539..10416
                     /locus_tag="AC16YU_07355"
     CDS             8539..10416
                     /locus_tag="AC16YU_07355"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6065077.1"
                     /translation="MEATTLVSSAGPIRTSDPRLGRTLPELLFDARDRYRTRNAFHRR
                     TESGWEVLSLDDFTRRAGHLALGLLESGLAEGDRVALFMDSDTAFCLVDFACQIAGLP
                     TVPLYLTQAPDVTRHILEHAGAATLVATDRERLGQVTEDLPPGSCRRLLLYEPDDGPR
                     EASGRTVGFCDEVEGIGAAIEASSPGSVRSLAARRRAHDPATIIYTSGTTGRPKGVML
                     SHENLSSNACFAYDSLTGYRPGPDGEVVLSFLPLSHVFARTLCVGAISRGTPLWFSQP
                     DRLKEDFPAVRPTMFATVPRVLEKIYAGIVDRTESASGVRRRLGRWGIKMARSHDVRL
                     GPGTGPGFRLVDRLVLARWRAAFGGRVRFAACGGAALDPVLANLFNAAGIPVLEGFGL
                     TETSPVITFNRPGRVRPGWVGEALPFVEVRIADDGEICTRGPHVMLGYYREPERTREV
                     IDPDGWFHTGDIGALSDDGYLKITDRKKALFKLSTGKYVSPQPLENRLTSDPLVEQAV
                     VVGEGFRFASALVFPAEEGVRRLSEALGLDVGLPFRALVEHPAVLGRFGALVDAANRG
                     MDPWSTIKRFRVVPDPVSVENGLLTPTLKVRRSNLQSRYAGLIRQMYEEGGSQAAPPG
                     V"
     gene            10525..10719
                     /locus_tag="AC16YU_07360"
     CDS             10525..10719
                     /locus_tag="AC16YU_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein CsbD"
                     /protein_id="HIR6065078.1"
                     /translation="MATATMNESWRRVKAQILTIWGDQEFGDAELKKARGNLGKMVEL
                     IHEKTGEPRAEIMQKISAFL"
     gene            10845..>11539
                     /locus_tag="AC16YU_07365"
     CDS             10845..>11539
                     /locus_tag="AC16YU_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6065079.1"
                     /translation="MASIGTPFESQRQASTAGWDELLETYARRVREIGLGGGEKRIRR
                     EHERGKMTARERIEALLDTPADFLEIGTFAGYGMYADEGGCPAGGTVMGLGHVSGRLC
                     MVVANDATVKAGAWFPITARKNIRAQEIALENRLPIIYLVDSAGVFLPMQEEIFPDRD
                     HFGRIFRNNAVLSARGIPQIAAIMGSCVAGGAYLPIMSDEALIVDGTGSVFLAGPFLV
                     KAAIGEEVDQETLG"
BASE COUNT         1899 a         3846 c         4032 g         1762 t
ORIGIN      
        1 tcatcatccg caacaaccgc ctcaagcgcc tgatcgacat caaggcgccg gaggtcatcc
       61 tgcggaacga gaagcggatg ctgcaggagg ccgtcgactc gctcttcgac aacagccgga
      121 aggcgaacgc ggtgcgctcg gactcgaacc gcgcgctcaa gtcgctctcc gacatgctca
      181 agggcaagca gggccggttc cgccagaacc tgctcggaaa acgcgtcgac tactccggcc
      241 gatccgtgat cgtcgtcggt cccgaactga aactgcacca atgcggtttg ccgaagaaga
      301 tggcgctcga actgttcaag ccgttcatca tccgcaagct catcgagcgc gggatcgtga
      361 agacggtcaa gagcgcgaag aaggtcgtcg atcggcgcac ctccgacgtg tgggacatcc
      421 tcgagaaggt gatccagggc cgtccggtgc tcctgaaccg ggccccgacc ctgcaccgcc
      481 tgggcatcca ggccttccag ccggtgctca tcgaggggaa ggcgatccag ctgcatcctc
      541 tcgtgtgtac ggccttcaac gccgacttcg acggggacca gatggccgtg cacgtcccgc
      601 tgtcgctcga cgcgacgctg gaggcgatgc tcctcatgct ctcgagccac aacatcctga
      661 gcccggcgca cggcggaccg atcacggtgc ccacccagga catggtgctc ggcctgtact
      721 acatgaccaa gtcccgcagc ggcatgcggg gcgagggcat gcgcttcagc agcatggccg
      781 aggcgcgcca ggccttcgac caggggatcg ctgaactgca tgcgaagatc aaggttcggc
      841 tctccgacgg cgagaccatc gagaccacga tcggtcgcgt gctcttcaac gagatcgtgc
      901 ccgaggaggt cggcttcatc aacgaggtgc tcacgaagaa gaacctccgt ctgatcatcg
      961 gccgtgtcct caaggcgtcc ggcttcccga agacggcccg gttcctggac gagatcaagc
     1021 aggccggctt cgagcgggcc accagcgcgg gcctcacctt ctcgctgacg gacatcgtcg
     1081 ttcccgacgc caaggagaag ctgatcagcc tggccgagac cgaggtcgac aaggcgcgcg
     1141 gcaactacgc gctcggcttc atcacggaga acgagcgcta caaccaggtc atcgacatct
     1201 ggacgcacac caacaaccag gtgtccgagg tcctgttcga gacgctgcgc accgaccgcg
     1261 agggcttcaa cgccatctac atgatggccg actcgggcgc ccggggctcg aaggagcaga
     1321 tccggcagct cggcggcatg cgcggcctca tggccaagcc gcagaagagc ctggtcggct
     1381 ccgctggcga gatcatcgag aacccgatca tctccaactt caaggagggg ctgtcggtgc
     1441 tcgagtactt catctcgacg cacggcgccc gcaagggtct cgccgacacc gccctgaaga
     1501 cggccgacgc cggctacctg acccgtcgcc tggtggacgt cgcgcaggac gtgaccgtca
     1561 cgcagacgga ctgcggaacg ctccgcggca tccggatctc ggccctgaag gacaacgagg
     1621 acgtggtcga gcccctggcg gaccgcatcc tgggccgcgt ttcggtccac gacgtcaacg
     1681 acccgctcac cggtgacctc atcgtctcgg cgaacgaggt catcgacgag gagaaggcgc
     1741 ggaggatttc cgagacctcg atcgaggagg tcgagatccg atcggtgctc acgtgcgaag
     1801 cgcggcgcgg agtctgcgcg ctgtgctacg ggcgtaacct ggccaccgga cggctggtcg
     1861 aggacggcga ggcggtcggc gtgatcgcgg cgcagtccat cggcgagccg ggcacccagc
     1921 tcacgctgcg aaccttccac atcggtggta cggccagccg catttcggca gagagcacga
     1981 tccagtccaa gtttgccggc atcgtcgagt tcgagaacct gcgcacggtc tcgcacgacg
     2041 acggcgagga gccgcgcgag gtcgtgctct cgcgccaggg cgagatccgc atcgtggacc
     2101 agggcgaggg acggcggcag ctgatcgctt acctgattcc gtacggcgcg gagatcctcg
     2161 tggcccgcgg ccaggtgatc gacaagggcg aggtgctcgc cagctgggac ccgtacaaca
     2221 gcgtcatcct gtccgaggtc tcagggcgcg tcgatttcca ggacatcctc gagggcacca
     2281 cgttccgcga ggagtcggac gagcagacgg gcttcaagga gaaggtcatc gtggattcgc
     2341 gcgagcggaa cctgactccg gccatcctga tcacgggcac ggacggaagc acgcgcgagt
     2401 acccgctccc ggtgcgcgcg cgcatccagg tggattccgg cgactcggtg caggccggcc
     2461 agatcctggt gaagatcccg cggcagtcgg ccaagacccg tgacatcacg ggtggcctcc
     2521 cccgcgtgac ggagctgttc gaggcgcgca caccgaccga tccggccgtc gtctccgaga
     2581 tcgacggcat cgtggccttc ggaggccgca agcgcggcgc ccaggaagtg gtcgtgacga
     2641 gccgcgacgg gaccgaatcc aagtcgtacc tggtctcgct gacaaagcac atgctcgtgc
     2701 acgacaacga cttcgtccga gccggagagc cgctctcgga cggccaggta tcgccgcagg
     2761 atatcctgcg catcctcggt ccccgcgccg tccaggagta tctcgtgaac gagatccagg
     2821 aggtgtaccg gctgcagggc gtgacgatca acgacaagca catcgagtgc atcgtccgcc
     2881 agatgatgca gaaggtgcgt gtcgcggacc cgggcgatac gaccctgctc gaggacgatt
     2941 acgtggaccg cttcatgctg gagcagctga acgacgatct ctacgacaac ttcgtcgtcg
     3001 tggaccccgg ggactccacc ctgcagatcg gcgacatcgt ggaccggagg cgcctgcgcg
     3061 aggtcaactc ggacctgaag cggcgagacg aggcccaggt ccaggagcgg gagacgcagc
     3121 ccgcggtcgc cgagccggtg cttctcggca tcacccaggc cgcgctgtcg accgattcgt
     3181 tcgtttcggc ggcgtccttc caggaaacga ccaaggtgct gaccgacgcg gcgatctcgg
     3241 cgaagaacga tccgctctac ggactcaagg agaacgtgat cgtcgggcac ctcatcccgg
     3301 cgggcacggg ccagcgccgg taccgccgga tcatggtcgg ctccaagcgc gagtacgagg
     3361 agatggtgct cggtgagggc gaaagcgcgg tgtccccgcg ggccggggac ggcaaggcga
     3421 ccgaggtcga ggcctgatcc ctgcacgctc cgtcgaatcg aagccccggg cgcagagcgc
     3481 gttcggggct tcgtcgttgg cgagggcccg tccgggcgcc ccccggttac ttccggggcc
     3541 tccgcggcgg ccttatgaaa gaaaacttcc ggccgatgca tccaattgga aagtcgtccg
     3601 tatggttgtt gcaacaacga atgacacggg acagcaacgg gacgatcatg ttacgggacg
     3661 cttatctgca ccttggactg ggcgggacga ttttggtcgg cggcctggtg atgctcacag
     3721 ccttctcctg gttcgtggcc atggcgggtg ccgtcacgcg tccgatgacc ccgcttcgcc
     3781 gcgggttgct tctcgcagtc ctgaccgcgc tccccccgtc ggccatcctg gtgttcgcac
     3841 gcttcgtggc cgtgacgcgc ggagagtatc gcacgcaccg taaggaggcc gctgccgcgg
     3901 gccgcgcgtc cgaactcatg ctgtacggct cccggctatc cgccgccgcc tgacgcgcgc
     3961 cgctatcccg ggcggacgag ctccgcgaaa tcccggttct cgatggcata ccgcggccag
     4021 tccacgaagt cgaagtgcca ccattcggcg cggtacactt cgaagccccg cccccgcatg
     4081 gtcgagatca gcaggtcccg gttggcgcgc cgatcggcgg gaccaccgtt ccagtccgcg
     4141 taagcacgct cggtgaactc gtcgtagccg ctgggcatgg cgagtggaat gcccgacgcg
     4201 aggtcgacga gccccacgtc cacggcgcag ccccggttat gccgcgatcc gagcgagggc
     4261 gtggcgacga aggtccggta ctcgggcggc gtggcctccc agaacagcca ggtcacccac
     4321 cacggccggt acgcgtcgta gacgacgagg cccaggccgc gagcctccag gtggtcctgg
     4381 acgagccgca gggcctcggc cgcgggccgc tgcagcaggg cctcttcctc cgggtagatc
     4441 tgcgtgccga gaaaattgtc ggatgacgcg taccggaggt ccatgaggat gcgcggatcc
     4501 atgtccgaca cccggacgaa gtcgggctcg cggaacggtc cgggctcttc gggcggctcg
     4561 gcctccctcg ccatggcgaa cagctcgtcc aggggatgca ggggctcgat gacgaaggtc
     4621 tcgctcacgg cgcggtcgtg atcagtccgg caggatggct tcggggaggc cggcggaatc
     4681 agccggcagg ccggtcgaat cggttgcggt cgaatcggta acggtcgaat cggtaggcgc
     4741 ggcgtcacgg cgcatgcgct cgaccgcctc gaaacgttcc cggacgttcg gatcggtggc
     4801 catctcgccc atctcgcggg cggcgaacac gccgagggcc gcggccgcca ggagcgcggc
     4861 cgtcaccagg aggcgcgaag cccagcgccg tagggggcgg atcgggacgg tgtcccggat
     4921 cgtgtcggga tcgcgagggg cgtccggcat gggagtggag gcggattccg gtcaagaccg
     4981 gttcggttcg cgcgcatggt acgagggcgg ggccgggcgg ttcgcgcggc caggccggca
     5041 gccgatccgt gcggcccggt ccgccgtggc gcggcaccag cgaacgggcg ggcccgggcc
     5101 ggctcagtcc agcgccccgg tccgggattc atgcccgtgc tccccccagt accggaccac
     5161 cgcccgcacg aagcgcaggg agcgctccag gctctgcacc gtgttccact cgaagcagga
     5221 gtggtagtcc atgcccccgt tgaagaggtt gggcgtcgga atgccggctt ccgacaggcg
     5281 ggagccgtcg gtgccgccgc ggacgcgctc cgcgcgcagc gtgatgccga tgccggcggc
     5341 ggcctcccgg gcgaagtgca ccgcgcgcgg gtcgatctcc tcgatgtacg accgcatgtt
     5401 gcggtactgg tccctgatct ccaggtcgat ggatgccgcg gggtactcca ggcggagcga
     5461 ctcgaccagg ctcgagacca gccgcttgcg ccgctcgagg ccgtcgtccg agaagtcgcg
     5521 caggatcagc cggacggatg ccctgcccac atcccccgcc gccagcgtat gcgggtggat
     5581 gaatccgcgg accccgtcgg tgtgctcggg cgactcggct gcggggagtg ccgcgatgag
     5641 ccgtccgacg atgcggacgg cgttcaccat gacgccccgc gcgtacccgg gatggacacc
     5701 cacgccgcgg accgtgaccg tcgcctccgc cgcgttgaaa gtctcgaagg agatcgtatc
     5761 gacgtcgctg ccgtcgatcg tgtacgcgat gcgggccgag agacttccga ggtccaggtg
     5821 gtcgacgcca cggccgattt cctcgtcgac ggtgaagcag agccgcacct cgggtcgggg
     5881 cgcggagtcg tcgacgaggt ccgcggcgag ctgcacgagg accgccacgc ccgccttgtc
     5941 gtcggacccg agaagcgtgg ttccgtcgct cgtgatgagg ttctccccga cgtgttcgag
     6001 gagggccggc tgcctggcgg gatcgagcgt caccgacgga ttgcccggca ggatcacggt
     6061 ctcgcccccg tacggcgggt gcacgatcgg tcgcaccccg gcgccgggcg cgtccggaga
     6121 cgtgtccatg tgtgcgagaa gtgcgacgac gggcagcgga tcgccgtccg gagccgaggc
     6181 ggtggccggc agcgtcgccc acacgtagcc gaattcgtcg atcgttgcgt cgaggccgat
     6241 tccggacagc tcggcctgga tgacgcgccc gaggtccttc tgcttctccg tcgacggaac
     6301 gttcgtcgag tccggatccg actgcgtgtc gatccgcacg taccgcagga accggtccac
     6361 gacgggtggc caggcagcgc cgctacccgc ttcggctgtc ttccgcattg cacatcacgg
     6421 ttatggccga cacgatctcg cgctcgcggt ccgagcccgc gggacgattc accagtcggc
     6481 cgttcacgac cagggcggag gagccgcccc cgtccaggtt catcgcccac accgccccga
     6541 ggtccctgag gatgaccgcg agctcctcga ggcggacccc ccggctccgc tgctggcggc
     6601 cgtccacgac cacgagcagc agctgtccgt ccgccgtgac acccgcggcg gagcgcggat
     6661 ggacgcgcgg gatgtaggcg tcgaagaagg cctcttcctc gcgcgtcacc ctgaactcgc
     6721 cgtcggacag gaggacgggg cccgcggaca gggcccactc cggaaaccag gcggcgcccg
     6781 atcggccgtc cggcgggatg gccggcctcc cgagggcgtt gtcgaacgga cgaccgaagc
     6841 cccgtatcgt gtcgtccgac gcgccggccc agcggatctg caggattccg gcggagtccg
     6901 ccccgatggt agcccgtacg gtgggatagc gcacctgctg ccagaccacg ccctccagcg
     6961 cggaggaaat ccgctcgcgg tcacgtacca ggaggccgat gtgccggcca cggcccgcct
     7021 cgtcgatccg gtagaatccg ccgttgatga gggcgcaggc cccggccgag cgtgccatct
     7081 ccgtgggcgt ggcgaggccg tcgtcgtccc cggcagtcgc cacgccgatc cggacgccct
     7141 cggccgtcgg gtcgacacgg atcgcccacg tccggagcgg caggtccggg ctcgttccgg
     7201 ccagcagccg tacgccgtcc gggaggcttc cgacgggggg cggcgtctcc gaccactcga
     7261 cccggatggg cgcgaagcgg tcggtaccgt cggcgcagcc accggacgcg agcagggctc
     7321 cgagggcgag gtgcagtatc gtggggcggg gcagagccaa gtccgggacg gtctgggccg
     7381 tgaaggtacg atccggacgg cagggcgtcc ggtcccgcgc gcgcggcccc ggcgtgcaac
     7441 tccgtcgcgg cggggccgta tcgacagagt gaaagcccaa attccactgc aaacatgcgt
     7501 ctcgccatcc gatcctcgat cctcgcaacg cttctgtcgg cgtgggtcct cccggcctgt
     7561 gttctggctg gaccgcagcc ctccgtcgac acgatcaagc ggagcttcca ggtgagccct
     7621 ggcggcacac tgcaggtgga tatcgacttc ggcaacgtga tcgtggagtc gtccggcgac
     7681 ggaatggtct acgtggaact ccagcgcacg gtgggctcgg agaacgagga cgaggccaag
     7741 cggctgctcg ccaggcacga atgggagatc gaccaggtcg gcaacgacgt cgtcgtggag
     7801 tcccggtacg agttcgaggg caccgaccgc tggatcaacc ggaatcgccg ggacaacgtg
     7861 ttccgcctca aggtcgtggt ccggatcccc gatgagtaca gcgtcgattt cacgacgggt
     7921 gccggcaacg tctccatcac ggatgtcggg ggctgggtgg agggcaccac cggagccgga
     7981 aacatcacca tcggcgaggt gacgggatcc gtggagatcc gctcgggatc gggcaacatc
     8041 gacatcgccg gaatcgacgg atcgatcgag gtcacctccg gagcgggcaa cgtcacgctc
     8101 ggctacgtga cgggcgagat ccaggcgaac acgggcgccg gcaacatcga ggcgcgcatc
     8161 acgaggcagc cgggcggtga ctcctccgtg gagacgggcg ccggcaacgt cacggtgtac
     8221 ctgggcagcg acgtcggcat gcaggtcgac gcccgggcca gcgtgggctc ggccgaatgc
     8281 gacttcggcc tgaaggtcag cgggaagtgg atgtcgaagt ccttcgaggg cgacgtgaac
     8341 ggcggcggtc ccgcgctgac catgcgatcc ggagtcggca acgtcgcgct gaagcgcatc
     8401 tgatccgaag cgagagaata tcgaacgggg cgcatccgac gggtgcgccc cgttctgcgt
     8461 tcggggggct tcggtggccg gaaccgcacg acccttcggc ggtgttcacc gggtccattc
     8521 cgtcgcggga gtcgatccat ggaagcgacg accctggttt cctccgccgg gccgatccgc
     8581 acttcggatc cgaggctcgg ccgcaccctg cccgaactgc ttttcgacgc gagggaccgc
     8641 taccgcacgc ggaacgcctt tcaccggcgc acggaatcgg ggtgggaggt cctctcgctg
     8701 gatgatttca cacgccgggc cggccacctc gcgctcgggc tcctggaatc gggactggcc
     8761 gaaggagatc gcgtcgccct gttcatggac agcgacacgg cgttctgcct ggtcgacttc
     8821 gcctgccaga tcgccgggct tcccacggtg ccgctgtacc tgacccaggc gcccgacgtc
     8881 acgagacaca tcctggagca cgcgggggcg gccacgctcg tggccacgga tcgcgaaagg
     8941 ctggggcagg tcacggagga cctgccgccg ggttcgtgcc gccgcctgct gctctacgag
     9001 cccgacgacg ggccccggga ggcgagcggg cggacggtcg gcttctgtga cgaggtggaa
     9061 ggcatcggcg cggcgatcga ggcgtcgtcg cccggcagtg tccgctccct cgcggcccgt
     9121 cgcagggccc acgatccggc cacgatcata tacaccagcg gaacgacggg ccggccgaag
     9181 ggcgtgatgc tcagccacga gaacctctcg tcgaacgcat gcttcgccta cgacagcctg
     9241 accggttacc ggccgggacc ggacggcgag gtcgtgctgt ccttcctccc gctgtcgcac
     9301 gtgttcgcgc gtaccctgtg cgtcggggcg atctcgcgcg gaacgcccct ctggttctcc
     9361 cagccggacc gcctgaagga ggatttcccg gcggtgcggc ccacgatgtt cgcgaccgtt
     9421 ccccgcgtgc tcgagaagat ctacgccggc atcgtggaca ggacggagtc ggcgtccggc
     9481 gtccgcaggc gcctgggacg gtggggtatc aagatggccc ggtcgcatga tgtccgcctc
     9541 ggacccggaa cgggccccgg tttcaggctc gtcgaccggc tggtccttgc ccggtggcgc
     9601 gctgcgttcg gagggcgtgt gcgtttcgcg gcgtgcggag gggcggcgct cgacccggtg
     9661 ctggccaatc tgttcaatgc tgcaggcatc cccgtcctcg agggcttcgg attgaccgaa
     9721 acgagccccg tcatcacgtt caaccgtccg ggtcgcgtgc ggcccggctg ggtgggcgaa
     9781 gcgcttccct tcgtcgaggt ccgcatcgcc gacgacgggg agatctgtac gcgcgggccc
     9841 cacgtcatgc tgggctacta tcgtgagccc gagcggacgc gggaggtgat cgaccccgac
     9901 ggctggttcc acaccgggga catcggtgcg ctctccgacg acgggtacct caagatcacg
     9961 gaccgcaaga aggccctttt caagctctcg acgggcaagt acgtgtcacc acagccgctg
    10021 gagaaccggc tcacgtccga cccgctggtg gagcaggcgg ttgttgtggg agagggcttc
    10081 cggttcgcct cggcgctcgt atttccggcc gaggaggggg tgcgccggct ttccgaggcg
    10141 ctcgggctgg atgtcggcct gcctttccgt gccctggtcg agcatccggc ggtgctcggc
    10201 cggttcggcg cgctcgtcga cgcggccaac cggggcatgg atccgtggtc gacgatcaag
    10261 cggttccggg tggtcccgga cccggtgtcg gtcgagaacg ggctgctcac tccgaccctc
    10321 aaggtccgcc gctcgaatct gcagagccgg tacgcggggc tcatccggca gatgtacgag
    10381 gaagggggca gtcaggccgc cccccccgga gtgtgaccgt aaggcgttgg cctgcacgga
    10441 atctgagatt ggattcggaa acctgggcgc gccgcgcgat gttcaacacc tgttggcagg
    10501 ttttcggcaa tgcacggagg gatcatggcg accgcgacga tgaacgagag ctggaggcgg
    10561 gtcaaggcac agatactcac catctggggg gaccaggagt tcggcgacgc cgagctgaag
    10621 aaggcccgcg gcaacctggg gaagatggtg gaactcatcc acgagaagac gggggagccc
    10681 cgtgccgaga tcatgcagaa gatctcggcc tttctctgac ggcggccttc cggctgcccg
    10741 cttcgcgagc gttcgaaagc ctcgccccac cgggcggggc tttcttcgta tgcgcgggtc
    10801 gcctacgttt cggctccccg tgaaacgccc tcgattcatc ccgcatggcc tcgatcggaa
    10861 cgccgtttga atcccagcgc caggcctcga ccgccggctg ggacgaactc ctggagacct
    10921 acgcccggcg ggttcgcgag atcgggctcg ggggcgggga gaagcgcatc cgccgcgagc
    10981 acgaacgcgg taagatgacg gcgcgcgagc ggatcgaggc gctgctggac acgcccgcgg
    11041 atttcctgga gatcgggacg ttcgccggat acgggatgta cgccgacgag ggcgggtgcc
    11101 ccgccggcgg caccgtgatg gggctcggcc acgtgagcgg caggctctgc atggtggtgg
    11161 ccaacgacgc cacggtgaag gccggcgcct ggtttccgat cacggcccgg aagaacatcc
    11221 gagcccagga gatcgctctc gagaaccgcc ttccgatcat ctacctggtg gattcggccg
    11281 gcgtcttcct gcccatgcag gaggagattt tccccgaccg ggaccacttc ggccgcatct
    11341 tccggaacaa cgcggtcctc tccgcccggg ggatcccgca gatcgcggcc atcatgggca
    11401 gctgcgttgc cggcggagcc tacctgccca tcatgagcga cgaggcgctg atcgtggacg
    11461 gcacgggctc cgtctttctg gccggtccgt tcctcgtcaa ggcagccatc ggggaggagg
    11521 tggaccagga aacgctcgg
//
LOCUS       DBNHQZ010000271         6915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1841830, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000271 DBNHQZ010000000
VERSION     DBNHQZ010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1841830"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="AC16YU_07370"
     CDS             <1..1482
                     /locus_tag="AC16YU_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065080.1"
                     /translation="FNMLMSGVREDYAGHRPERRYVDYRDGRFSYRAVGASSGPVLLE
                     AVGDVLDSRHRVTGKMELIRRAPIAALTFDGPLGNVDSRGTSWTITGLDDDIPSQVLS
                     GMQTRSHGVRSILEQADQAAQAEMPAAQVTGVAGVGDFVQNPGLVTFHDIVEMVDAMP
                     ASEKTVIAGPVSWTGSQTYGSPSDPAVVVVEGDLTIAGNVTGYGILIVKGALDIRQNG
                     NPVWEGLFIVESSKTTHKMLGNPQIYGAMAMIATTTAGETGGEADAGMLGGHFDVTVL
                     DGAGNVRYQAHQYDDRYDVTSVNVLKPGCRQGGLCWEDLVLNSGESQVKVDVLSGSSS
                     FVQINGGATEAGTWIAPDAIATLSIGGFGLCDLPNSSPQAVESSPSNGQAVRVTGQSG
                     TVLYTVSVYHHTVSGTCAYESVPGTYFEAVRPMNFTIGGTAKINMSSVALERLYTGFP
                     LLASADPEYVLSNVRELAENTSTELFTPTTEMDKLIDTRDFFY"
     gene            1946..3178
                     /locus_tag="AC16YU_07375"
     CDS             1946..3178
                     /locus_tag="AC16YU_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR6065081.1"
                     /translation="MKSTFRPPDLPEICREIAARIPYSHVGDDRILFVIDQIHKISAA
                     QRMELRKYMDALVEHTLRLGASDIDFGGPAANGWIWYRVDGDKTPHKEIGNLSIDETT
                     VLILSVLAESARNTLMEERAIDFSYSLPREGTRNARFRATAYFDLDSLALNMRAIPEQ
                     TRSLASLEFHPLIEKGLMFRHVRDGLTLVTGVTGSGKSTTLDAVIDANNDDVEAHVVI
                     IAKPVEFMHESRKCIIRHREVGRDVRSFKDGIVHSLRQDPDIVVIGEMRDPATISAAL
                     EITDSGHKVFSTLHTSSAVESLDRIVAEYPPDEQDRVRNRLADVLRCVISQKLLPRVG
                     GGRIMAKEVLWMTPSSRAAIKNQNTGEIYQMMWEGWNQGMITLEQDLFRLMRQGRITT
                     DTAINFANNKRRLNQLFK"
     gene            3209..5032
                     /locus_tag="AC16YU_07380"
     CDS             3209..5032
                     /locus_tag="AC16YU_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR6065082.1"
                     /translation="MSTTPVLGITVSSQTVVGVLVDQRDDGPAVVRTMYRSRSSSAAK
                     SAAQKPRVLSPETSAEEDPDVHFSTAGGAGDLFLAGEFSIGGPAGSTPGVVGSPVAPS
                     TVEFEVKELVAECAEAGFPKVQVVFATGMPYLNTHEIRPGRGDEDEGEKADRRKRSDR
                     AARPGRRRKRSARHDALVRQLVDEVDGPVNQDAVLFLTMTPTAENEERFLAVYARPDE
                     PLLPTLRLLEQRGRGVPNVDLVDTEVSLLLGLGRLAAPAEEAGRRRRLVLRVGIEDAV
                     ALYLDGQRLHHFEKLRSITVYDPPETIGSRILLLQDEYGIGEADEILVLSEENEEALI
                     AFLSDFFPNSDVRSANSLLPPYPGDPAYRWSRQAALAASAALRAIHERGSRSLFETVN
                     LAPGRSRRRRGDRKGVAWQVYAMFGLLFGMSLFFVARYMAQQHDIDMAELRLSQFSPE
                     EIERSVGSLESRIDSIQNRIDGFIYALDVLDSLLVGSDKWSRQMEHTASQTASVPGIW
                     VETWTDQESTLELVGNATSRDQIVRLATRMDGVIESVTFSEIRDWPVYSFRMTVPVTV
                     EIPEAARFLREQAASVPETATVPVRQASDPAPASPDEATSK"
     gene            5049..6047
                     /locus_tag="AC16YU_07385"
     CDS             5049..6047
                     /locus_tag="AC16YU_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065083.1"
                     /translation="MGIYNNILVIGMCWVLTAGLGSYLTFFRQPDQLTVLLKQEQDLK
                     LRRLEIEELLAKEMATEAQAREILARWNARYKLIPDEMETTDVVGYLNSLTSSGYKAF
                     DVSYGGPAPGRAFSRHVFKVSGRGFFTSLYQTVWAIENHRDFYRVENLNLNHFDLTYE
                     DPFTGRPRMDVLVTFTFEVHAYFGGMDGLSADDLGLFASSDAIVVQPQGQLPPVPESV
                     LPDRQPAKNPFHPLILQTIPPNTDNLPEIEDATLVSIVENRAVFILSGSKKMVELGVG
                     DPVYLGQITAVDPREGVVIARLNKGGIIDEIEMVLESGERYRQAFGPNMLTPSESR"
     gene            6075..>6915
                     /locus_tag="AC16YU_07390"
     CDS             6075..>6915
                     /locus_tag="AC16YU_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065084.1"
                     /translation="MTRYRNPMAIRVIAVTLLVLGGALGAPAGSQAQDMPPQRQLRTY
                     IPPDQLVSFLPSTPFSSFIELLSPTFERVTGKQLIDPESRDFPIGVPIQSSHFFDAME
                     LVLYHNRLTYRETDKYFIVSEVGPDEQLMVGGDRTASSAKPVDKPATLATRQVQINAV
                     LFEVNHTRARDIGFDWSAIVGSAGQQGGGGGSGGGSQQNQSRVPFYIDTHDMFGDNSR
                     IVAPDQIAYSDINSIIRFAENQGIGETIASPGVSVQSGVKGRIQVGSDVPVQIKDFSG
                     NTIT"
BASE COUNT         1211 a         2353 c         2264 g         1087 t
ORIGIN      
        1 ttcaacatgc tcatgtccgg cgtccgcgag gactacgcgg ggcatcgccc cgagcgtcgc
       61 tacgtggact accgcgacgg ccggttctcg taccgcgcgg tcggcgcgtc ctcgggcccc
      121 gtgctgctcg aggccgtcgg cgacgtgctc gattcaaggc accgggtcac gggcaagatg
      181 gagctcatta ggcgcgcacc gatcgcggcg ctgacgttcg acggcccgct ggggaacgtg
      241 gactcccgcg gtaccagctg gacgatcacc gggctcgacg acgacattcc gagccaggtg
      301 ctgagcggca tgcagacccg ctcgcacggc gttcggagca ttctcgaaca ggccgaccag
      361 gccgcgcagg ccgaaatgcc ggcggcccag gtgaccgggg tggccggtgt cggcgacttc
      421 gtgcagaacc cgggcctggt gaccttccac gacatcgtcg agatggtcga cgcgatgccg
      481 gcctccgaaa agaccgtgat cgcgggcccc gtcagctgga ccggcagcca gacctacggc
      541 tcgccgtccg atccggccgt cgtggtggtc gagggcgacc tcaccatcgc gggcaacgtg
      601 acgggatacg gcatcctgat cgtgaagggc gcgctcgaca tccgccagaa cggcaacccg
      661 gtgtgggagg gactcttcat cgtggagagc tccaagacca cccacaagat gctcggcaat
      721 ccgcagatct acggcgcgat ggcgatgatc gcgacgacga ccgccggcga gaccgggggc
      781 gaggcggatg ccggcatgct cgggggccac ttcgacgtca cggtcctcga cggcgcgggc
      841 aacgtgcgct accaggccca ccagtacgac gaccgctacg acgtcaccag tgtgaacgtg
      901 ctcaagccgg gctgccggca gggtggcctg tgctgggagg acctggtcct gaattcgggc
      961 gaatcccagg tgaaggtgga tgtcctctcc gggagcagct cgttcgtgca gatcaacggc
     1021 ggcgccacgg aggccggaac ctggatcgcc ccggacgcga tcgcgacgct ctcgatcggc
     1081 gggttcggac tctgcgatct gcccaattcc tcgccgcagg cggtcgagag cagcccgtcg
     1141 aatggccagg ccgtccgcgt gacgggccag agcggcaccg tcctctacac cgtgtcggtg
     1201 tatcaccaca cggtgagcgg cacctgcgcg tacgagtccg tgccgggtac gtacttcgaa
     1261 gcggtccggc cgatgaactt caccatcggc ggtacggcga agatcaacat gagctccgtc
     1321 gccctcgagc gcctgtacac cgggttcccg ctgctcgcgt ccgcggaccc cgagtacgtc
     1381 ctgagcaatg tgcgggagct cgccgagaac accagcacgg aactgttcac gccaacgacg
     1441 gaaatggaca agctcatcga cacgagagac ttcttctact aggatcgaag gatcgttcgt
     1501 ggccgggatc gtgagcccgg cgcggggagc gggccgtcgg agcgaatccg gcggcccgct
     1561 tcgtcgtttc gggtagatca cggagcgtcg gcgcctggcg tccggccacc gggcggtgcg
     1621 ttgtcctttc agccgggccc ggagacggtc ccgcacgctc ctcccgcatc gttcggacca
     1681 tcggcttaac ccgttcgtcg aacggccgat acgacagttg ttggatcggg cctatcaccg
     1741 gcccggaaaa caggaaaaaa ccagaatgga acgcatcccc gacgacatca ggccggtcgc
     1801 aggaaccggc caatcgcgcc cctcggcgtc ggcttcggcc gccgctgcga cttcagggtc
     1861 ggcggctccc gaaacgggag cgcatgccgc ccccgagtcg gtagcgtccc gcgttgccgc
     1921 cgcccgaaag gcggccgacg tgaaggtgaa gagcaccttc cgtcctcccg atctccccga
     1981 gatctgccgc gaaatcgcgg cccgcattcc gtactcgcac gtcggcgacg accgcatcct
     2041 gttcgtgatc gaccagatcc acaagatctc ggccgcccag cggatggagc tgcgcaagta
     2101 catggacgcc ctggtcgagc acacgctgcg gctcggtgcg tccgacatcg acttcggcgg
     2161 cccggccgcc aatggctgga tctggtaccg cgtcgacggc gacaagacgc cgcacaagga
     2221 gatcggcaac ctctcgatcg acgagacgac cgtactgatc ctgtcggtgc tcgccgagag
     2281 cgcccggaat accctgatgg aggagcgcgc gatcgacttc tcctattcgc tcccgcgcga
     2341 gggcacccgc aacgcccgct tccgcgcgac cgcctacttc gacctggact ccctggcgct
     2401 caacatgcgc gccatcccgg agcagacccg gtcgctcgca tcgctcgagt tccatcccct
     2461 gatcgagaag ggcctcatgt tccggcacgt gcgtgacggg ctgaccctcg tgacgggtgt
     2521 cacaggttcc ggcaagagca cgaccctgga cgcggtgatc gacgcgaaca acgacgacgt
     2581 ggaggcccat gtcgtcatca tcgcgaaacc ggtcgagttc atgcacgagt cgcgcaagtg
     2641 catcatccgc caccgcgagg tggggcgcga cgtccggagc ttcaaggacg gcatcgtgca
     2701 ctccctgcgc caggaccccg acatcgtggt catcggcgag atgcgcgacc cggccacgat
     2761 ctcggcggcg ctcgagatca cggactcggg tcacaaggtg ttctcgacgc tgcacacctc
     2821 gtccgccgtc gagagcctgg accgtatcgt ggcggagtat ccgccggacg agcaggaccg
     2881 tgtccggaac cgtctggccg acgtgctccg gtgcgtgatt tcgcagaagc tgctgccgcg
     2941 cgtcggcggc ggccggatca tggccaagga ggtgctctgg atgaccccgt cgtcccgcgc
     3001 cgccatcaag aaccagaata ccggcgagat ctaccagatg atgtgggagg gctggaacca
     3061 gggcatgatc accctggaac aggatctctt ccgactcatg cgccagggcc ggatcacgac
     3121 cgatacggcc atcaactttg ccaacaacaa gcggcggctc aaccagctgt tcaagtagca
     3181 gccggcgggc ccgcagccga atcgatccat gagcaccacc cctgtactcg gcataacggt
     3241 cagcagccag accgtggtcg gcgttctcgt cgatcaacgg gacgacggac ccgcggtcgt
     3301 ccgcacgatg taccgcagcc gttcctcgtc ggcggcgaag agcgcggccc agaagccgcg
     3361 tgtcttgtcg ccggagacga gtgcggaaga ggatccggac gttcacttct cgaccgcggg
     3421 tggggcgggc gacctgttcc ttgcgggcga gttctcgatc gggggtccgg cgggaagcac
     3481 gccgggcgtg gtcgggtccc cggtcgcgcc gtccacggtc gagttcgagg tgaaggaact
     3541 ggtcgccgaa tgcgcggagg ccggattccc gaaggtccag gtcgtgttcg ccaccggcat
     3601 gccgtacctg aacacgcacg agatcaggcc gggccgcggg gacgaggacg aaggagagaa
     3661 ggcggaccga aggaagcgga gtgaccgggc cgcgcgcccg ggtcgccgac gcaagcgttc
     3721 ggcgcggcat gacgccctgg tgcggcagct ggtggacgag gtcgacggtc ccgtgaacca
     3781 ggacgcggtg ctcttcctga cgatgacgcc gaccgcggag aacgaggagc ggttcctggc
     3841 cgtgtacgcc cgtcccgacg agcccctgct gcccacgctc cggctgctcg agcagcgcgg
     3901 tcgcggggtg cccaacgtgg acctcgtcga cacggaggtc agcctcctgc tcgggctcgg
     3961 ccgcttggcc gccccggccg aggaggcggg gcgccggcgc cgcctggtgc tgcgcgtggg
     4021 catcgaggat gccgtggccc tgtacctgga tggccagcgg ctccaccact tcgagaagct
     4081 ccgctccatc accgtttacg atcctcccga gaccatcggg tcccgcatcc tgctcctgca
     4141 ggacgagtac ggcatcggtg aggccgacga gatcctggtg ctcagcgagg agaacgagga
     4201 ggcgctcatc gccttccttt cggacttctt cccgaactcc gacgtccgca gcgcgaactc
     4261 gctcctgccg ccgtatcccg gcgatccggc gtaccgctgg agccggcagg cggcgcttgc
     4321 ggcctcggcc gcgctccgcg cgatccacga gcgcggctcg aggtcgctgt tcgagaccgt
     4381 gaacctggcg ccgggcaggt cccgccgccg tcgcggcgac cggaagggcg tggcctggca
     4441 ggtctatgcc atgttcggac tgctcttcgg catgagcctc ttcttcgtgg cccgctacat
     4501 ggcgcagcag catgacatcg acatggccga gctgcgcctg tcgcagttca gccctgaaga
     4561 aatcgagcgt tccgtgggct cgctcgaatc ccgcatcgac tcgatccaga accggatcga
     4621 cgggttcatc tacgcgctcg acgtgctcga ctcgctcctc gtgggcagcg acaagtggag
     4681 ccgccagatg gagcatacgg ccagccagac ggcctccgtg ccgggcatct gggtcgagac
     4741 ctggacggac caggagtcga ccctcgagct ggtcggcaac gcgacgagcc gggaccagat
     4801 cgtgcgcctc gccacccgca tggacggcgt gatcgagagc gtcacgttct ccgagatccg
     4861 cgattggccc gtctacagct tccgcatgac cgttccggtc accgtggaga taccggaagc
     4921 cgcgaggttc ctgcgcgagc aggccgcgag cgtcccggag actgcgaccg taccggtccg
     4981 ccaggcctca gacccggcgc cggcctcccc cgacgaagca acctcgaagt gacccaccgg
     5041 aaacgaccat gggtatctac aacaatattc tcgtgatcgg tatgtgctgg gtcctcaccg
     5101 caggactcgg ttcctacctc accttcttcc gccagccgga tcagctgacc gtgctcctca
     5161 agcaggagca ggatctgaag ctccgtcggc tcgagatcga ggagcttctc gccaaggaaa
     5221 tggccacgga ggcccaggcg cgggagatcc tggcccgctg gaacgcgcgc tacaagctga
     5281 ttcccgacga gatggagacc accgacgtcg tgggctacct caacagcctc acctcgagcg
     5341 gatacaaggc cttcgacgtc tcgtacggcg ggccggcgcc cggccgtgcc ttcagccggc
     5401 acgtgttcaa ggtcagcgga cgcgggttct tcacgagcct gtaccagacg gtctgggcca
     5461 tcgagaacca ccgggatttc taccgggtgg agaacctgaa cctgaaccac ttcgacctca
     5521 cgtacgaaga tccgttcacc ggtcggcccc ggatggacgt actggtcacg ttcaccttcg
     5581 aggtgcacgc gtacttcggc ggcatggacg gcctttcggc ggacgacctc ggactcttcg
     5641 ccagcagcga cgcgatcgtg gtccagccgc aggggcagct gccgcccgtg ccggagtcgg
     5701 tgctgcccga ccgccagccg gccaagaacc cgttccatcc cctcatcctt cagaccattc
     5761 cgccgaacac ggacaacctg cccgagatcg aggacgcgac gctggtgtcc atcgtcgaga
     5821 accgggcggt attcatcctg agcggctcga agaaaatggt cgagctcggg gtcggcgacc
     5881 ccgtctacct cggccagatt accgcggtcg atcctcgcga gggcgtggtc atcgcgcggc
     5941 tgaacaaggg gggcatcatc gacgagatcg agatggtgct cgaatcgggt gagcgttatc
     6001 gccaggcctt cgggcccaac atgctcaccc catcggaatc tcgctaggca ccaatctcct
     6061 ttcggaacac cgtcatgact cgctacagaa acccgatggc gatccgcgtg attgccgtga
     6121 cgctcctggt gctgggcggc gctcttggcg ccccggccgg atcgcaggcg caggacatgc
     6181 cgccgcagcg gcagctccgc acctacatcc cgccggacca gctggtttca ttccttccgt
     6241 cgacgccctt ctcgtcgttc atcgagctgc tgagcccgac cttcgagcgc gtcaccggga
     6301 agcagctgat cgaccccgaa tcccgggact tcccgatcgg cgtaccgatc cagagcagcc
     6361 acttcttcga tgcgatggag ctcgtgctct atcacaaccg gctcacctac cgggagaccg
     6421 acaagtactt catcgtgtcg gaagtgggcc ccgacgagca gctcatggtc ggcggtgatc
     6481 ggacggcgtc ctccgcgaag ccggtcgaca agcctgcgac gctcgcgacc cggcaggtcc
     6541 agatcaacgc ggtcctcttc gaggtgaacc acacccgggc ccgtgacatc ggcttcgact
     6601 ggagcgccat cgtggggagt gcgggtcagc aggggggtgg cggcggctca ggaggcggaa
     6661 gccagcagaa ccagagccgc gtgcccttct atatcgacac tcacgacatg ttcggggaca
     6721 actcccgcat cgtcgcgccc gaccagatcg cgtactccga catcaactcg atcatccgct
     6781 tcgccgagaa ccagggcatc ggcgagacca tcgcgtcgcc cggcgtcagc gtgcagagcg
     6841 gcgtgaaggg ccggatccag gtgggttcgg acgtgccggt ccagatcaag gacttctcgg
     6901 gcaacacgat caccc
//
LOCUS       DBNHQZ010000272         5476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2789438, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000272 DBNHQZ010000000
VERSION     DBNHQZ010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2789438"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16YU_07395"
     CDS             complement(<1..308)
                     /locus_tag="AC16YU_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065085.1"
                     /translation="MKGRAALGTSLVVVFATVGVMVGTAAAALLAGPGIQALMVRAVV
                     VTLVIFLAILFARYFALLWFAYLGHAERNVMGLRDLREFPPVSIILPAYNEGRVIERA
                     L"
     gene            complement(305..2596)
                     /locus_tag="AC16YU_07400"
     CDS             complement(305..2596)
                     /locus_tag="AC16YU_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065086.1"
                     /translation="MTPRPENRSGGAGYFLHFTGIALLLVASTLLLVLVVLPRRYVLS
                     AGFRESSLTFPTSRAPFAPPPTVHVPARRLATSTPSGASAGPGPAEALWARVLPPLRS
                     GRAHEALPIFAAYLEQYPSDHDARREYAVALVRADRAVEAVAVQEKLLEETRDPGVRL
                     LLARTLRDLGRVDDASAHYAALSRTAPADVALALEWSRAFSVESRYGPAENVLRDALA
                     RDSSDASLRVELARIRYYTGRLAEADDVLSSLPEADLDDLGARQLRDDVAAARAVPDG
                     PAADPSPPSLLERAVAAREDDDFATAARLFEEALATDPTAADAWRAYADLLQYEMADP
                     AGAHDALVTVESLSEFSPDLQIRLARLELWLDRPARAEARLVAFLSDREPEGAGPAPP
                     PATRTEAHVMLGDLYRWDGRRRPAAVQYGRALAAAPDDERAREGLEQLRQDAFLGTVE
                     SQRPRVGVTATSLFDTDGVHRWETSGEWVGVSEAWTWTVRAGTRSVHGSDLLGAAATD
                     RGRFADTEVGRWWRWGTLRTALRLGAEDLRAEGAQVVLGASVRLSGPDGGRTDVSLGR
                     EPAHLRTNTLQSMRGGLVEQRLAVSHARPIGTRWSLATNVEGALLDAGPVVQTDAGIR
                     WQGSLSVGRVVTPSVTLGLSTGASGFTRPAPRPGGPRLYWDPRSALSFGPYVRLDHAL
                     DERWSVNGALQTGVAHLDERSPFEASLVPHLSAEAGVRREGARYRVAMDAFYLQGRFD
                     GYRAYGLTLAFGARTGPFRRSTR"
     gene            complement(2580..4169)
                     /locus_tag="AC16YU_07405"
     CDS             complement(2580..4169)
                     /locus_tag="AC16YU_07405"
                     /inference="COORDINATES: protein motif:HMM:NF018455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HIR6065087.1"
                     /translation="MATILTPDGPTALRPSERPLRWASSEPVETPTIPSGIAPLDDRE
                     GGLKRGGSYLIVGPPGPPKRVAALQFLLRGLEESENGVLVTHADAAGILEVARAWGFD
                     MFDAWHEGRLRIVGFRDDFELRAVRSIAPEEVVEELDALVGHDVARVAIDPGAMFLNG
                     GAKTLLGAAFARWAMRHAATVVSTFSVDGPGTALPSSADWLHHVTTGRLVLEPGARGF
                     VDLTLACPVPGVHRNDAPITLELKSGGGLVAPTSSPARRGADRDAVDPRRLLVLSLGP
                     AQSADLESWARRSFDPDLVSEPFDAVTRLQFADVYGGVVVQASRERIRAAVQACRALR
                     PLTRAPIVFASDDSIRSTDRVQILEAGADDCLSGGLDFRELEVRIKQAIAAGAKPPQE
                     SRPAEAPSPVPSTSDARSVSPAVLAAEVERRAADPALAFFCLLEVTADLARTELAETL
                     VGQVRQEEGDLVSPGDHGCLVLLQGARASQLDPFLQRLRSGIARRTGMPATDLGIQVL
                     SHPTDRGRIRMLTGTLRDAAT"
     gene            complement(4175..>5476)
                     /locus_tag="AC16YU_07410"
     CDS             complement(4175..>5476)
                     /locus_tag="AC16YU_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6065088.1"
                     /translation="ELGHDVLSVDSATAALNSIADFGPDVVLSDVMIPGKSGLELLSH
                     LRNLTPDIDVVLFTGQGDAQGAIDAMQLGASDYLTKPLCLDEAEGVLSQCMLRRERAR
                     TTSAAPTGAADPEPAFGLVGRHPSMVHVYKNIGPMARAGSGPVLIRGETGTGKELIAR
                     AIHHTSGRTGPFVAVNCAAVPEALLESELFGHVRGAFTGATMDRRGRFEAAAGGTIFL
                     DEIGDTTPAFQAKVLRVLQEREFYPVGSDQPRISDAAVIAATHRPLREMVDEGTFRED
                     LFYRLQGLEIHVPPLRDRRSDIPLLVRHIAARASKEAGLAPPVIPPQVIDELSRREWC
                     GNVRELENVLRRAVVTSRGSALSLRDIDAVPARSHEGRDPAATNPGPATLSEVRSTVE
                     RRHVQYVLNDVDGNKSRAARILGVSRPTLNRLIQDHHLALP"
BASE COUNT          801 a         1913 c         2059 g          703 t
ORIGIN      
        1 agcgcccgct cgatgacccg tccctcgttg taggccggca ggatgatcga cacgggcggg
       61 aactcccgca ggtcccgcag gcccatgacg ttccgctcgg cgtggccgag gtaggcgaac
      121 cagaggaggg cgaagtagcg ggcgaacagg atggccagga agatgacgag ggtcacgacc
      181 acggcccgca ccatcaacgc ctggatgcca ggacccgcca ggagggcggc ggcggcggtg
      241 ccgaccatga cgcccacggt cgcgaagacg accaccagcg acgtgccgag ggcggcgcgg
      301 cccttcatcg ggtgctccgg cggaacggcc cggtgcgggc gccgaaggcg agggtcagcc
      361 cataggcacg gtatccgtcg aagcgtccct gcagatagaa cgcgtccatg gccacgcggt
      421 agcgcgcccc ctcccggcgg accccggcct cggccgacag gtggggcacg agcgacgctt
      481 cgaaggggct ccgctcgtcg agatgcgcca cacccgtctg gagcgctccg ttcacggacc
      541 agcgttcgtc gagcgcgtgg tcgagccgga cgtacgggcc gaacgagagg gcgctccggg
      601 gatcccagta cagccggggc ccgcccggac ggggcgccgg ccgggtgaag ccgctggcgc
      661 cggtggacag gcccagcgtg acggacggcg tgacgacgcg gcccacggac agcgatccct
      721 gccagcggat gccggcatcg gtctgcacca cagggccggc gtcgaggagc gccccttcca
      781 cgttcgtggc caggctccag cgggttccga tgggccgtgc gtggctcacc gccagacgtt
      841 gctcgaccag tccgccgcgc atgctctgca gcgtgttcgt ccgcagatgc gcgggctcgc
      901 ggccgaggct gacgtccgtg cggccgccat ccggccccga gagccggacg gacgcaccca
      961 gcaccacctg ggcgccctcg gcgcggaggt cctccgcccc gaggcgcagc gcggtccgga
     1021 gggttcccca gcgccaccag cgtcccacct cggtgtcggc gaagcggccg cgatcggtgg
     1081 cggcggctcc gagcaggtcg gatccgtgga cggatcgggt tcccgctcgg acggtccagg
     1141 tccacgcctc ggaaacgccg acccattcgc cgctcgtctc ccatcggtgg accccgtcgg
     1201 tgtcgaagag ggacgtggcg gtgaccccga cgcgcggacg ctggctctcc accgttccga
     1261 ggaacgcgtc ctgacggagc tgctccaggc cctcgcgggc ccgctcgtcg tccggcgccg
     1321 ccgccagggc tcgaccgtac tgcaccgcgg caggccgtcg acggccgtcc caccggtaca
     1381 ggtcgccgag catgacgtgg gcctcggtcc gcgtggcggg aggaggggcg ggacccgcgc
     1441 cctcgggctc gcggtcggac aggaacgcca cgagtcgcgc ctccgcccgg gccggacggt
     1501 cgagccagag ttcgagccgg gccagtcgga tctgcaggtc cggggagaac tccgacagcg
     1561 actcgacggt gaccagggcg tcgtgggccc ctgccggatc cgccatctcg tactggagga
     1621 gatccgcgta cgcgcgccat gcatcggcgg ccgtgggatc ggtggcgagc gcctcctcga
     1681 agagccgggc cgccgtggcg aagtcgtcgt cctcccgcgc ggccacggcg cgctcgagga
     1741 gggagggcgg cgaagggtcc gccgcgggcc cgtcgggaac cgcgcgggcc gcggcgacgt
     1801 cgtcgcggag ctgtcgcgcc ccgaggtcgt ccagatcggc ttccggtagc gaggacagga
     1861 cgtcgtcggc ttcggcgagt cgaccggtgt agtaccggat gcgcgcgagt tccacgcgga
     1921 gggacgcgtc ggaggagtcc cgggccaggg catcgcggag gacgttttcg gccggaccgt
     1981 agcgggactc gaccgagaag gcgcgggacc attcgagtgc gagggccacg tcggccggtg
     2041 ccgtccgcga cagcgccgcg tagtgcgccg aggcgtcgtc cacccgaccg aggtcgcgga
     2101 gggtccgggc gagaaggagc cggacccccg ggtcgcgcgt ctcctccagc agcttctcct
     2161 ggaccgccac ggcctcgacg gcgcggtccg cccggaccag cgccacggcg tattcgcggc
     2221 gggcgtcgtg gtcggacggg tactgctcga gatatgccgc aaagatggga agagcctcgt
     2281 gggcgcggcc gctccgcagc ggagggagga cccgcgccca gagggcctcg gccggtccgg
     2341 gcccggcgga tgccccggag ggcgtggacg tggcgagtcg acgcgccgga acgtgcaccg
     2401 tcggcggggg ggcgaacggc gcccgggagg tcgggaacgt gaggctggat tcacggaagc
     2461 cggcgctcag cacgtaccgg cgcggcagga ccaccagcac cagcagcagg gtggaggcca
     2521 ccagcagcag cgcgatgccc gtgaagtgca ggaagtaccc cgcacccccg gagcggttct
     2581 caggtcgcgg cgtcacggag ggtaccggtc agcattctga tcctgccgcg atccgtcgga
     2641 tggctcagga cctggatgcc caggtccgtg gccggcattc ccgtccggcg cgcgattccg
     2701 ctccggagcc gctgcaggaa ggggtcgagc tgggaggccc gggcgccctg gaggagaacc
     2761 agacagccgt ggtcacccgg agacaccagg tcgccctcct cctggcggac ctgcccgacc
     2821 agggtttcgg ccagttcggt ccgcgcgagg tcggcggtca cctccaggag gcagaagaac
     2881 gccagcgctg ggtcggccgc gcggcgctcg acctccgcgg ccaggacggc cggggacaca
     2941 ctccgggcgt cggacgtcga cgggaccggg gacggcgctt cggccggacg cgattcctgc
     3001 ggaggcttgg cgcccgccgc gatcgcctgc ttgatgcgca cctccaactc ccggaagtcg
     3061 aggcccccgc tcagacagtc gtcggccccc gcctcgagga tctggacgcg gtcggtggac
     3121 cggatggaat cgtccgacgc gaagacgatg ggcgcccgcg tcagcgggcg gagggcccgg
     3181 caggcctgga cggccgcgcg gatccgctcc cgcgacgcct ggacgaccac gccgccgtac
     3241 acgtccgcga actgcaggcg cgtcacggca tcgaacggct cggacaccag gtccggatcg
     3301 aagctgcggc gggcccagga ctccaggtcg gccgactgcg ccgggccgag ggacaggacc
     3361 aggagtctcc ggggatccac ggcgtcccgg tccgcgccac gtcgggcggg ggaggacgtg
     3421 ggggccacca gccccccacc ggacttgagc tcgagggtga tgggcgcgtc gttccggtgc
     3481 acaccgggaa ccgggcaggc cagcgtaagg tcgacgaacc cgcgggcacc cggctcgagg
     3541 acaaggcggc ccgtggtcac gtggtgcagc cagtcggccg aggacgggag cgccgtcccg
     3601 gggccgtcca ccgaaaaggt ggacaccacc gtggcggcgt gccgcatcgc ccaccgggcg
     3661 aaggccgcgc cgaggagcgt cttggcccca ccgttgagga acatcgcccc gggatcgatg
     3721 gccacccggg ccacgtcgtg gcccaccagc gcatccagct cttcgacgac ctcctccggc
     3781 gcgatcgagc ggacggcccg cagttcgaag tcgtcccgga agccgacgat gcggagcctg
     3841 ccctcgtgcc acgcgtcgaa catgtcgaat ccccaggcgc gtgccacctc caggattcct
     3901 gccgcgtcgg cgtgggtcac gagcacgccg ttctccgact cctcgaggcc gcgaagaagg
     3961 aactggagtg cggccaccct cttgggcggg cccggaggcc cgacgatcag gtaggatccg
     4021 ccccgcttca gccctccctc gcggtcgtcg agcggggcga tgccggaggg gatcgtgggc
     4081 gtctccaccg gctcgctcga ggcccaccgg agcgggcgtt cggaaggacg aagggccgtt
     4141 gggccgtcgg gggtcaggat cgtggccatg gtcgttacgg cagtgcgagg tggtggtcct
     4201 ggatcagacg attcagggtg ggtcgcgaga cgcccaggat cctggccgcc ctgctcttgt
     4261 tgccatcgac gtcgttcagc acgtactgca cgtgccggcg ctccaccgtg gaacgcactt
     4321 cggagagggt ggccggcccc gggttcgtgg ccgccgggtc gcgtccctcg tgtgaccggg
     4381 ccggcacggc atcgatgtca cggagggaaa gggccgagcc gcggctggtg accaccgcgc
     4441 gccgcagcac gttctcgagc tcgcggacgt tgccacacca ctcgcggcgc gacagttcgt
     4501 cgatgacctg gggagggatc accggcgggg ccagccccgc ttccttggag gcgcgcgcgg
     4561 cgatgtgccg gacgagaaga ggaatgtcgg accgccggtc gcgcaggggc ggcacgtgga
     4621 tctcgagtcc ctggagccga tagaagaggt cctcgcggaa ggtcccctcg tcgaccatct
     4681 cgcgaagcgg ccgatgcgtt gccgcgatga ccgcggcgtc gctgatgcgc ggctggtcgc
     4741 tgcccacggg atagaactcc cgctcctgga gtacgcgcag caccttggcc tggaaggccg
     4801 gggtcgtgtc tccgatctcg tccaggaaga tcgtgccgcc cgccgcggcc tcgaatcgcc
     4861 cgcgccggtc catcgtggcg ccggtgaagg caccgcgaac gtgcccgaac agttcggatt
     4921 cgagcagggc ttccggcacc gcggcgcagt tcaccgccac gaacggaccg gtccgtccgg
     4981 acgtgtggtg gatggctcgg gcgatcagct ccttgccggt tcccgtctcg ccgcgaatca
     5041 gtacgggccc cgaccccgca cgcgccatgg ggccgatgtt cttgtagacg tgcaccatgg
     5101 acggatggcg tccgaccaga ccgaaggccg gctccgggtc cgcggctccc gtcggcgccg
     5161 ccgaggtggt tcgggcgcgc tcgcgccgca gcatgcactg gctgaggacc ccctcggcct
     5221 cgtcgaggca caggggcttc gtcaggtagt ccgacgcgcc gagctgcatg gcgtcgatcg
     5281 ccccctgggc atcgccctgt cccgtgaaca ggaccacgtc gatgtccggg gtgaggttcc
     5341 tgaggtggga aaggagctcc agcccgctct tgccgggaat catgacgtcc gataggacga
     5401 cgtccggccc gaagtcggcg atgctgttca gggccgcggt cgcggagtcg acgctcagga
     5461 cgtcgtgacc gagctc
//
LOCUS       DBNHQZ010000273         4670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3196456, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000273 DBNHQZ010000000
VERSION     DBNHQZ010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3196456"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC16YU_07415"
     CDS             <1..752
                     /locus_tag="AC16YU_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065089.1"
                     /translation="TGYPMVKHARDMVRSGEIGDVRKIVVEYPQGWLAELVEVTGNKQ
                     AEWRADPTRAGVSSALGDIGSHAENLARYITGLHLEEVHADLTTFVPGRKLEDDANIL
                     VHYQGGARGILYASQVSVGDENNLRIRVYGTRASLEWKQEHPNWLHVRGHEIPERVLK
                     RGNPYLSDAAAHNTRLPWGHPEAFIEAFANIYVNAGRTIAAKLAGDAPGPFDTDFPTV
                     QDGAIGVHFIHAAVRSGRERTWVDARYTPPS"
     gene            781..1779
                     /locus_tag="AC16YU_07420"
     CDS             781..1779
                     /locus_tag="AC16YU_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6065090.1"
                     /translation="MARPVTLFTGQWADLPLETLAAKASEWGYDGLELACWGDHFDVR
                     RALEDPGYCRGRLDLLAEYELGVWAVSNHLVGQAVCDRIDGRHRSILPAHVWGDGDPE
                     GVRQRAAREMMDTARAAAKLGVPVVNGFTGSSIWHLLYSFPPVLPEQVEQGFQDFADR
                     WNPILDVFDEVGVRFALEVHPTEIAFDIASAERAVAALGGRGAFGFNYDPSHFGYQGV
                     DYVGFIDRFAERIHHVHMKDVWWSATPGEAGVFGGHTDFGDRRRFWDFRSLGRGSIDF
                     EEIIRALNRVGYSGPLSVEWEDAGMDREHGAEEACAFVQSVDFPPSGRAFDSAFSE"
     gene            complement(1786..3759)
                     /locus_tag="AC16YU_07425"
     CDS             complement(1786..3759)
                     /locus_tag="AC16YU_07425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065091.1"
                     /translation="MRRLLALALALFLAAPLASAQDLTIGVAVEGTLNPDSRDVYSLA
                     THAGYYVYGHVNQLTVDVVVRILDPEGEEVAQFDSPARGYEPFHFSPDSEGVYSVEVT
                     PFEGAEGEYVVELIANEPKATEPVALVDQLMTPFSGTDRPGAVISVLEDGEVVMARGY
                     GMANLTHGVPMTDTTGMSIASVSKHFTAMAILLLEQDGRLSLDDDVRRHIPELKDFGT
                     PVTLRHLLNHTSGYREILNFLPITGSQATDAMDRMEPIRVIQRQARLQNEPGAEYNYN
                     NTAFMLLATVVERVSGQGFREFMEERIFGPLGMENTTVKIYQGQVIPNSSQGYTNAEG
                     GGFRAVTDFASAYGASGVNSTARDMTKWMLNFRDGTVGGPEAIAKLTTRGVLTGGDTT
                     GYALGLGVRTWRGQQLYTHTGGETSHRTWFGYFPEIQSGLFFSSNNPAFGLGMWEDLA
                     EAFFGEHLEPKEEEPAAGPDDSTAHATPSTEQLEAIAGTWSFVGAPLLIEYTVQDGRL
                     FAQATGQPRFRVDPTSDSTFTFVGVEASVTFHFEADGSVSRATHHQNGDTPLEKVTEP
                     PLTPEQLLAFEGRYWSDELETLYTLTVEDSVLTAHHRRLEPFGLTHRTGDEFSGGMWF
                     FGTVRFDRDPTGSVSGFLASNGRTRDVWFVRLR"
     gene            3884..>4670
                     /locus_tag="AC16YU_07430"
     CDS             3884..>4670
                     /locus_tag="AC16YU_07430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6065092.1"
                     /translation="MVRSTEPDRSADALETRVTELEQALGERDAALARADRDSQVEAA
                     VERIRGQVLGMRESSDLLDIVVTMHREFSGLGFEAGYFWHMRWKPTTYEKAMTSGDGS
                     RIGMVMDLPRGFHNVPEMTAWERNDEPVGIFRFDVDGALDYVHRMIDQGRFHEIDPNA
                     PSDEDIRHIGGITFVMARTTHGEIGYSLPGTADPPPEATAVLVRFARAFDLAYRRFED
                     LLRLTEEKARTEHALAELRVMQAQLVEQEKLASLGALTAGIAHE"
BASE COUNT          731 a         1579 c         1618 g          742 t
ORIGIN      
        1 acacgggcta cccgatggtc aagcacgccc gcgacatggt gcgctcgggc gagatcggcg
       61 acgtccgcaa gatcgtcgtc gagtatccgc agggatggct ggccgagctc gtggaagtca
      121 cggggaacaa gcaggcggaa tggcgcgccg atccgacccg ggccggcgtg tcgtccgcgc
      181 tcggagacat cgggtcccac gcggagaacc tggcccgcta catcacgggg ctgcacctcg
      241 aggaggtcca cgcggacctg accaccttcg tgccgggacg aaagctcgaa gacgacgcga
      301 acatcctcgt gcactaccag ggaggcgcgc gcggcatcct gtacgcgtcc caggtctccg
      361 tcggggacga gaacaacctg cggatccggg tctacgggac gcgggcctcc ctggagtgga
      421 agcaggagca cccgaactgg ctccacgtcc gcgggcacga gatccccgag cgcgtgctga
      481 agcgcggcaa cccgtacctg tcggacgccg ccgcgcacaa cacgcgcctg ccctggggcc
      541 atccggaggc gttcatcgag gcgttcgcga acatctacgt gaacgccggg cgcaccatcg
      601 ccgcgaagct cgcgggcgat gccccaggcc ccttcgacac cgactttccg accgtgcagg
      661 acggcgcgat cggcgtccac ttcattcacg ccgccgtgcg ctcgggccgc gagcggacgt
      721 gggtggacgc ccgctacacg ccgccctcct gagaccgcac tttcattcga ggacttcgac
      781 atggcacgcc ccgtcacgct gttcaccggc cagtgggccg atcttccgct cgaaaccctg
      841 gccgccaagg cctccgagtg gggctacgac ggcctggaac tcgcctgctg gggcgatcac
      901 ttcgacgtgc gcagggcgct cgaggacccg ggctactgcc gggggcggct ggacctcctg
      961 gccgagtacg agctcggcgt ctgggccgtt tcgaaccacc tggtggggca ggccgtctgc
     1021 gaccgcatcg acgggcggca ccgttccatc ctccccgccc acgtgtgggg agacggcgat
     1081 ccggagggcg tccgccagcg ggccgcccgc gagatgatgg acacggcccg ggccgcggcg
     1141 aaactgggcg ttcccgtggt gaacggcttc accgggtcgt cgatctggca ccttctgtat
     1201 tctttcccgc ctgtgctgcc ggagcaggtg gagcagggat tccaggactt cgcggaccgc
     1261 tggaacccga tcctcgacgt gttcgacgag gtcggcgtcc ggtttgccct cgaggtgcac
     1321 ccgaccgaga tcgcgttcga catcgcctcg gccgagcggg ccgtggcggc gctcggcggc
     1381 cgcggggcgt tcggcttcaa ttacgacccg agccacttcg ggtaccaggg cgtggactac
     1441 gtgggcttca tcgaccggtt cgccgagcgg atccaccacg tgcacatgaa ggacgtctgg
     1501 tggtcggcca cgccgggcga ggccggcgtc ttcggcgggc acacggactt cggcgaccgc
     1561 cgccggttct gggacttccg ctcgctcggg cgcggatcga tcgacttcga ggagatcatt
     1621 cgcgcgctga accgcgtggg ctattccggc ccgctctcgg tcgagtggga ggacgcgggc
     1681 atggaccgcg agcacggcgc cgaggaggcg tgtgcgttcg tgcagtccgt cgacttcccg
     1741 ccgtcgggtc gcgcgttcga ctcggcgttc agcgagtagg cgggcctacc ggagcctcac
     1801 gaaccagacg tcgcgcgtcc gcccgttgga agcgaggaag ccgctcacgg acccggtcgg
     1861 atcgcggtcg aaacggaccg ttccgaagaa ccacatgcct ccggagaact cgtcgcccgt
     1921 ccggtgggtc agcccgaaag gctcgagccg gcggtggtgc gccgtcagca cgctgtcctc
     1981 gacggtgagc gtgtacagcg tctcgagctc gtcggaccag tagcgtccct cgaaggccag
     2041 gagctgttcc ggggtgagtg gcggctcggt gaccttctcg agcggggtgt cgccgttctg
     2101 gtggtgcgtg gcgcggctca ccgagccgtc ggcctcgaag tggaacgtca cggacgcctc
     2161 gacgccgacg aacgtgaagg tcgagtcgct ggtcggatcc acccggaacc ggggctggcc
     2221 cgtggcctgg gcgaagagtc gcccgtcctg gaccgtgtac tctatcagga gcggagcgcc
     2281 gacgaagctc cacgtgccgg cgatcgcctc gagttgctcc gtggacggag tggcgtgcgc
     2341 ggtcgagtcg tcgggaccgg cggcgggctc ctcctctttc ggctccaggt gctccccgaa
     2401 gaaggcctcg gcgaggtcct cccacatccc caggccgaac gccggattgt tgctcgagaa
     2461 gaacagcccg gactggatct ccgggaagta cccgaaccac gtgcggtgcg aggtttcgcc
     2521 acccgtgtgg gtgtacagct gctgcccacg ccacgtgcgg acccccagcc cgagggcgta
     2581 accggtggtg tcgccgcccg tgagtacccc ccgcgtcgtg agcttcgcga tggcctcggg
     2641 cccgccgacc gttccgtccc ggaagttcag catccacttc gtcatgtcgc gggccgtgga
     2701 gttcacgccg ctcgcgccgt aggccgaggc gaaatccgtc acggcccgga atccgccgcc
     2761 ttcggcattc gtgtatccct gcgagctgtt cgggatgacc tggccctggt agatcttgac
     2821 ggtcgtgttc tccatgccga ggggtccgaa gatccgctcc tccatgaact cacggaaccc
     2881 ctggcccgag acgcgctcga cgaccgtggc gagcagcatg aacgccgtgt tgttgtagtt
     2941 gtactcggcg ccgggctcgt tctgcagccg cgcctgccgc tggatcacgc ggatcggctc
     3001 catccggtcc atcgcgtcgg tcgcctggct gccggtaatg ggcaggaagt tcaggatctc
     3061 gcggtagccc gacgtgtggt tcagcaggtg acgcagcgtc acgggcgtcc cgaagtcctt
     3121 cagctccgga atgtgccgtc tcacgtcgtc gtccagcgac agcctgccat cctgctcgag
     3181 cagaaggatc gccatcgccg tgaaatgctt cgagacggac gcgatgctca tgccggtggt
     3241 gtccgtcatc ggcacgccat gggtgaggtt ggccattcca tagcccctcg ccatcacgac
     3301 ctcaccgtcc tccaggaccg agatcacggc ccccggacgg tccgtgcccg agaacggggt
     3361 catcaactgg tccaccagcg cgaccggctc ggtggccttg ggctcgtttg cgatgagctc
     3421 caccacgtat tcgccctcgg ccccctcgaa cggcgtgacc tcgaccgagt agacgccctc
     3481 tgaatcgggc gagaaatgga acggctcata accgcgggcc gggctgtcga actgggcgac
     3541 ctcctctccc tcggggtcga ggatgcggac cacgacgtcc accgtcagct ggttcacgtg
     3601 cccgtacacg tagtagccgg cgtgcgtcgc cagggagtac acgtcccggg aatcgggatt
     3661 gagcgttccc tcgaccgcga cgccgatcgt gaggtcctgg gcggaggcga gcggcgcggc
     3721 gaggaagagg gcgagggcga gggcgaggag gcggcgcatg ggactcgggc ttcgatcgtt
     3781 tcggtccgga caagatatcg gacggcggct tccgctgcgc tgcccgccgc cggccgattt
     3841 ccgcccggaa agtcgtatcg tggctcagca attgcgagac ccgatggtgc gatccacgga
     3901 accggatcgg agcgcggatg cgctcgagac ccgcgtcaca gagctcgaac aggcactcgg
     3961 cgaacgcgac gccgccctcg cgcgggccga ccgcgacagt caggtcgaag cggccgtgga
     4021 acgcatccgc ggccaggtcc tgggcatgcg ggagtcgagc gatctgctcg acatcgtcgt
     4081 gaccatgcac agggagtttt cgggactcgg tttcgaggcg gggtatttct ggcacatgcg
     4141 ctggaagccg accacctacg agaaggccat gacctccggc gacggctcgc ggatcggcat
     4201 ggtcatggac ctgccgcgcg gcttccacaa cgtgcccgag atgaccgctt gggagcggaa
     4261 cgacgagccg gtcggcatct tccgcttcga cgtggacggg gcgctcgatt acgtccaccg
     4321 catgatcgac caggggcggt tccacgagat cgatccgaac gctccttcgg acgaggacat
     4381 ccggcacata ggcggcatca cgttcgtgat ggcgcgcacg acccacggcg agatcggcta
     4441 cagcctgcct gggaccgccg atccgccgcc cgaagccacg gcggtgctcg tgcggttcgc
     4501 cagggccttc gacctcgcct accgccggtt cgaggacctg ctccggctga cagaggagaa
     4561 ggcccgcacg gagcacgccc tcgcggagct gcgggtcatg caggcccagc tcgtcgagca
     4621 ggagaagctg gcgtcgctcg gcgcgctgac ggccgggatc gcgcacgaga
//
LOCUS       DBNHQZ010000274         3532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_352665, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000274 DBNHQZ010000000
VERSION     DBNHQZ010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_352665"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..2665
                     /locus_tag="AC16YU_07435"
     CDS             380..2665
                     /locus_tag="AC16YU_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6065093.1"
                     /translation="MLRRLVAGLRARLFPQSYEKRLDGLFERAIGQPRERRASFVAEA
                     CADDPLLLEDVTSLLEAHDAAGQYFDHLAADVVGPALSALTRPFEEATGPDVVDRLQA
                     ALAGTYRLDRELGGAGMSRVFVAEDLRLKRRVVIKVLPPDMARAAKKDLFRREIELAA
                     QLQHPHIVPLLSADSADGMLYYTMPFVAGESLGDRLAREGPLPLDDALRIWRDVLDAL
                     AHAHASGVVHRDIKPGNILLSGRNALVADFGISRAIETAAGEAWALMSGPVIGTPAYA
                     APEQVEGDVGADHRVDIYAAGLVMYEMLEGRPPFSAASAADYAHAHRTIDPPVLNRPE
                     APRELIGLVARCLAKAPSERPRSAEAVVAELDDAASDPGRAGLAKNAASPARRVASWV
                     LLALALPAAMMIAFLQLKESSDIAGAGKGVPALASVPRRGPSSDPAAYARLRTSSPAA
                     YEWYERGMDVSLMRTDSGKRQGVEYFRRAISLDSSYIDAHAGLVRMYLQLSNAAPSAE
                     KKAWFDLAEQSALTAVAIDDADAGGRAALGWVRLLARDFSEAEAEFKRAIALDPAVPR
                     VHEGLSRVYMMTDRPAEQLAEAQLGVEADPFSHSAIREHALALASNGRCEESLDQLES
                     LKRLSPPAGVAGVVSGLCHASREMWAEAIAEFRWAGEHSATAGPAFLGFALARAGRTD
                     EAREILADLVTGRKFSHGSFGIAVVHAGLRDFDQAFVWLDRAAEDRTMHFYIAHPAFG
                     EIHRDPRFEALRRRVGGPRRP"
     gene            complement(2675..>3532)
                     /locus_tag="AC16YU_07440"
     CDS             complement(2675..>3532)
                     /locus_tag="AC16YU_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065094.1"
                     /translation="VSDRDGTRDIYAVDADGTGLVRLTADVWRDEAPAWSPDGSRIAF
                     SRTGAIHVMGADGSSLSRRVTGSEPAWSPDGSAIAYSTLHGGQVRIALLADADSSVSY
                     VTSTPGYNSQPSWSPDGRHLAYVSDWAAYDFANDIYSIGADGAGRQQFTHGVAYWPEI
                     QHFLHPAWSPNGRLIAFVYGTMSDGLNGNYLLGYMRYRVAVMPANGGSVTDLAWAGDI
                     PWIELLDPGSLAWSPDGSGIAYTFVDCNLAQESDCSKVRSVKYVSLDLYEQGTLVENA
                     ESPSWTWRR"
BASE COUNT          554 a         1144 c         1241 g          593 t
ORIGIN      
        1 gacgggcggt ggcggggcgc gggttccggg tccgtgcgac ggggtagtct cgcgtacccc
       61 gcgagcggaa cgtcacggcc aaccttgctt cgcgacggcg aatgtggttc cttggctcgt
      121 gctacccccg tgtcgccctg tgctgcatgg ttcttctgac gtcgcgtgag tgaatcggcg
      181 tacgaacgcc ctggcggtgc ttgcgcgatg atgcagacga gagtgagttt tttcggaccg
      241 gacgccagcg caccggcgtt cgggcggcga cgcggaagga ggcgcatcac ctccttcggc
      301 catggcacgg atacgacgaa aaccgggaaa aatcgatgcc gctcagaggt tcgttgcggc
      361 cggcttcgct tacgggccgg tgctccgacg ccttgtcgcc ggtctccggg cccggctgtt
      421 cccgcagtct tacgagaagc gcctcgacgg cctgttcgag cgcgcgatcg ggcagccgcg
      481 cgaacggcgt gcctccttcg tagcggaggc gtgcgctgac gacccgttgc tcctcgaaga
      541 cgtgacctcg cttctcgagg cgcacgatgc cgcgggccag tacttcgacc acctggcggc
      601 agacgtggtt gggcccgcgc tgtcggcgtt gacgcggccg ttcgaggaag ccaccggccc
      661 cgacgtcgtg gaccggctcc aggccgcgtt ggccggtacc tatcgcctgg accgggagct
      721 cggcggggct gggatgagta gggtgttcgt agccgaggat ctccggctca agcggcgggt
      781 cgtcatcaag gtgctgccgc cggacatggc ccgtgccgcc aagaaggacc tgtttcgtcg
      841 tgagatcgag ctcgctgccc agcttcagca cccgcatatc gtgccgctgc tgtcagccga
      901 ttccgcggac ggcatgctgt actacacgat gccgttcgtc gctggcgaat ccctcggcga
      961 ccggctcgcc cgggagggtc cgctgcctct cgacgatgcg ctgcgcatat ggcgagacgt
     1021 cctcgacgct ctagctcatg ctcacgcgtc cggcgtggtt caccgcgaca tcaagccggg
     1081 caacatcctg ctcagcggac gaaacgcgct agtcgctgat tttggcatct cccgggcgat
     1141 cgaaaccgcg gccggcgagg catgggcgct gatgtccggc cccgtgatcg gtaccccggc
     1201 atacgcggcg ccggagcagg ttgagggcga tgtcggggcg gatcatcgcg tcgacatcta
     1261 cgcggccggc ctggtgatgt acgaaatgct ggaaggccgc ccgccgttca gcgcggcctc
     1321 cgcggctgat tacgcgcatg cacaccggac gattgacccg cccgtactga acaggccgga
     1381 ggccccccgg gagctgatcg ggctcgtggc gagatgcctt gcaaaggcac cctccgagag
     1441 gccgcggagc gcggaggcgg tggtcgcgga gctcgacgat gcggcgtcgg atcccgggcg
     1501 agcgggtctg gcgaagaacg cagcctcgcc ggctcgacgc gtcgcatcct gggtgctgct
     1561 cgcgctggcg cttcccgccg cgatgatgat cgcgtttctg caactgaagg agtcgtccga
     1621 catcgccggt gccgggaagg gagtgcccgc actcgcctcc gtgccgagaa gaggtccgag
     1681 ctccgatcct gctgcctacg cccgtctccg cacctcgagt cccgcggcat acgagtggta
     1741 cgaacgcggc atggacgtgt ccctgatgcg cacggacagc ggcaagaggc agggagtcga
     1801 gtacttcagg agggctatct cgctcgattc gagctacata gatgcacacg ccggcctcgt
     1861 tcgcatgtac ctgcagctct cgaacgcagc gccgagcgcc gagaagaagg cgtggttcga
     1921 tctggccgag cagtcggcgc tcacggcggt cgcgatcgac gatgccgacg ccggcggtcg
     1981 ggcggcgctt ggctgggtac gccttctggc cagggacttc tcggaggcgg aagcggaatt
     2041 caagcgcgcc atcgccctgg accccgcggt gccgcgtgtg cacgaaggcc tctcgcgcgt
     2101 gtacatgatg acggatcggc cggccgagca gttggcagag gcgcagctcg gggtcgaggc
     2161 cgatccgttc tcgcattcgg cgatcaggga gcacgcactt gccctcgctt ccaatggccg
     2221 gtgcgaggag tcgctcgacc agctcgagtc gctgaagagg ctgagccctc cggccggagt
     2281 cgccggggtc gtcagcggcc tgtgccatgc gagccgggag atgtgggcgg aggcgatcgc
     2341 ggagttccga tgggcagggg agcactcggc gacggccggt ccggctttcc tcggtttcgc
     2401 gctggcgcgg gcagggcgga cagacgaggc cagggagatc ctcgcggacc tggtgacggg
     2461 acggaagttc agccacgggt ccttcggcat cgccgtggtc cacgccggcc tgcgggactt
     2521 cgaccaggct ttcgtgtggc tcgaccgggc ggcggaggac aggacgatgc atttctacat
     2581 tgcccaccca gcattcggcg agatccaccg ggatccgcgg ttcgaggcgc tcaggcggcg
     2641 cgtgggaggc ccgaggcggc cctgaccccc gggctcatcg acgccaggtc cacgaggggc
     2701 tctccgcgtt ctcgacgagc gtgccctgct catacaggtc gagagagacg tacttcaccg
     2761 accggacttt ggagcagtcg gattcctgcg ccaggttgca gtcgacgaac gtataggcga
     2821 ttccgcttcc gtccggcgac cacgcgaggg agccgggatc gaggagttcg atccagggaa
     2881 tgtcgccggc ccacgccagg tccgtcacgg atccaccgtt cgccggcatg actgcgaccc
     2941 tgtagcgcat gtacccaagg agatagtttc cgttcaaccc gtcggacatg gtaccgtata
     3001 cgaaggcgat cagccggccg ttcggcgacc aggcgggatg caggaagtgc tgtatctcgg
     3061 gccagtaggc cacaccatgg gtgaactgct gccgacccgc cccgtcggcg ccgatcgaat
     3121 agatgtcgtt cgcaaagtcg taagccgccc agtcgctcac gtacgcgagg tgccgaccgt
     3181 ccggagacca cgacggctgg gaattgtacc cgggagtcga ggtcacgtag gatacgctcg
     3241 agtcggcatc cgccaggagc gcgattctca cttgtccgcc gtgcagggtc gaatacgcga
     3301 tggcggatcc gtcaggggac catgcaggct ccgacccggt cacccttcta ctgaggctcg
     3361 agccgtcggc ccccatgacg tgaatcgcgc cggttctgga gaaggcgatt cggcttccgt
     3421 cgggcgacca cgccggcgcc tcgtcgcgcc agacgtccgc cgtgagacgc acgagaccgg
     3481 ttccgtccgc gtcgacggca tatatgtcgc gggtgccgtc gcgatccgat ac
//
LOCUS       DBNHQZ010000275         4497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2790155, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000275 DBNHQZ010000000
VERSION     DBNHQZ010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2790155"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16YU_07445"
     CDS             complement(<1..418)
                     /locus_tag="AC16YU_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR6065095.1"
                     /translation="MPVRVAVDAMGGDHAPAVVVDGCVEAARALGPDVEILLAGPAGD
                     IEPMLRERDAGGLPIRVLDAPDVIAMAESPSTALKSKPRSSIHVGLAACRSGDADAFV
                     SAGNTGAVLAASVVVLGRLAGVSRPAIIGYYPTVRSF"
     gene            complement(507..698)
                     /gene="rpmF"
                     /locus_tag="AC16YU_07450"
     CDS             complement(507..698)
                     /gene="rpmF"
                     /locus_tag="AC16YU_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR6065096.1"
                     /translation="MANPRRKTSKARSRSRRANYYSRLEAPTTVECPNCGDTHLMHRA
                     CPSCGQYRGRQVVERKDVA"
     gene            complement(735..1247)
                     /locus_tag="AC16YU_07455"
     CDS             complement(735..1247)
                     /locus_tag="AC16YU_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR6065097.1"
                     /translation="MAPVNVFALEIADFKSGTRTVELSPAPEDLDLDPEVFRDIRIVA
                     HLDRTGDRIFAQLEVSGTARLQCDRTLVDYDQDLEGRYGVLFASAEELERLGSDQDEV
                     REFDPASDELDVTEAVRDTLLLSIPLRRIAPGADDVPIRTSFGPAEDEAGDPRWDALR
                     SLRDPSPDES"
     gene            1916..3406
                     /gene="dnaA"
                     /locus_tag="AC16YU_07460"
     CDS             1916..3406
                     /gene="dnaA"
                     /locus_tag="AC16YU_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR6065098.1"
                     /translation="MSQSPEAVWKACLEVIRDNVSRQSFKTWFAPLKGVKLDETEDGK
                     KLTVQLPSRFYYEWLEEHYYGLLRKTVTKVLGPGGRLFYDIVIEKDDAESGYDGASMH
                     LPARRPANEPQPTQATLDAPRPGAAVAPFPSPGPGPVASPFVIPGIQKIHVESRLNPS
                     YTFDRFIEGDCNRLARSASLAIAQHPGGTSFNPFLLYGGVGLGKTHLIQAIGNYIEAN
                     QSGATTLYTSSERFTTEFVQAIQHNRVSDFSLFYRQIDLLIIDDVQFFSGKEKTQEEF
                     FHIFNALHQSGKQIVLSADRPPKDIQGIEERLLSRFQWGLSADLQLPDLETRTAILQR
                     KSEEDGIDVGPDVIEFIAHNVKSNIRELEGALIRLLAHSTLHKRELDLPLARDVLRDL
                     IKESRISLTIEEIQRLVCTYFSIPEDLVRAKTRKREVVQARQIAMFFSKQFTQHSLKT
                     IGLHFGGRDHSTVIHANQSVENQIETDPKFRMMMDEIRHRIDLHSR"
     gene            3530..>4497
                     /gene="dnaN"
                     /locus_tag="AC16YU_07465"
     CDS             3530..>4497
                     /gene="dnaN"
                     /locus_tag="AC16YU_07465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR6065099.1"
                     /translation="MKFTAASSDLLKAFQTVAGAVPTKSTLPILECILFEADGNTLRL
                     SATDLEISIRQKLAVTLKGSNGAPTRVAVPAKRLLDTLRALPEVPVEFEADGDFNVSL
                     RTDRGRYKMVGFDGADFPALPDLSEKTRIETSGALLRRAITKTSFAVSRDALRPAMMG
                     IYFQIGSDEGRAVATDGHRLVKLTLHDMTAPENTHFVVPEKAMSLAARVATDDPCGIT
                     VDGGYVAFDFGRSRVLARLIDEPYPNYQAVIPIDNEKTLVVSRESMLAAVKRVALYSS
                     TMTNQIRLSISANEVRVSAEDIERASEAQETVAGEFQGEDMVIGFNA"
BASE COUNT          760 a         1578 c         1452 g          707 t
ORIGIN      
        1 cgaagctccg gacggtcggg tagtagccga tgatcgccgg ccggctcacg cccgccagcc
       61 ggccgagcac cacgaccgag gccgcgagca cggcgccggt gttgcccgcg ctcacgaagg
      121 cgtcggcgtc acccgagcgg caggcggcga gccccacgtg gatggaggat cgcggcttgg
      181 acttcagcgc cgtcgagggc gactccgcca tggcgatgac gtcgggcgcg tcgagcacgc
      241 ggatcggcag gcccccggcg tcacgctccc ggagcatggg ctcgatgtcg cccgccgggc
      301 cggcgagaag gatctccacg tccgggccga gcgcgcgggc cgcctccacg catccgtcca
      361 ccaccaccgc cggggcgtga tcacccccca tggcatcgac ggctacgcga acgggcatgg
      421 cgggcaggga ttactggtcg aggggatacc gtgtcgcgcc ccaaggtacg acttcgggcg
      481 aatccgcggc tcgccgcgag ggtcgctcag gcgacgtcct tgcgctcgac cacctgcctt
      541 ccgcggtact ggccgcagct cgggcaggca cggtgcatca ggtgcgtatc gccgcagttc
      601 gggcactcca ccgtcgtggg cgcttcgagg cggctgtagt agttggcacg ccggcttctg
      661 gagcgggcct tggatgtctt tcggcgcggg ttcgccatct cggtaccttg acggtgggat
      721 tcgtatcgcc gcgatcagct ctcgtccggg gacgggtccc gcagggaacg cagggcgtcc
      781 caccgcggat ctcccgcctc gtcctccgcg gggccgaacg aagtcctgat ggggacgtcg
      841 tcggctccgg gcgcgatgcg gcgcaggggt atggacagca gtaaagtgtc gcgaacggct
      901 tcggtgacgt cgagttcgtc cgaggccgga tcgaattccc ggacctcgtc ctggtcgctg
      961 ccgagccgct cgagctcctc ggccgacgcg aacagcacgc cgtaccgacc ttcgaggtcc
     1021 tggtcgtaat cgaccagcgt acgatcgcac tgcaggcgtg ccgttccgga gacttcgagc
     1081 tgagcgaaga tgcggtctcc cgtccgatcc aggtgggcga cgatccggat atcccggaac
     1141 acctccggat cgaggtccag gtcctcggga gccggcgaca gctcgaccgt ccgggtaccc
     1201 gacttgaagt ccgcgatctc cagcgcaaac acgtttaccg gagccatact tttcacagag
     1261 ccgaataata tacgacgatg caatcggacg agccaaaccg gcagcctcgc ggccgcttca
     1321 aattctggtt gaggggccgt ccgtcgactc tgtgaaatcc ggctccgctt ccttgctgtt
     1381 cgggggtggg catgttacca tgcgaccacc gacgcacctg cgccggaccc cctgcccacc
     1441 agggaccggc gttccgggtt tcggacacgg cctgagccgc cgacggcgtc aggccgagtc
     1501 tcttgcgggc ccccatgccg tgtcggccgc gtgtgcggca cgggcccgtc cggcccccgg
     1561 atgcgggtgt ggacaacctg tagaaaacct cggtttagcg ggaagtcatg cggtcccgag
     1621 tcgacgccga ggggctttgc gcgtcccggc acgaaggacc gataatcggt caagttcttt
     1681 gttctcaagc aattgcagcc gccgccgctg tggtctggcc gattcgccgg ggaccggatc
     1741 gggtccggac cccgtttttc ggccgatttt cggcgatgtg gaaaagcggt gagaaaaccg
     1801 tcggccgcgt gcgttccgcc tcgcgccatt ccattttcac ggacgggcgc gaagtcccgc
     1861 gcccgcagcc gatcgcccgg gccccttccg ggcatgagcc gacgtcgttc cgtccatgag
     1921 ccagtctccc gaagccgtct ggaaagcttg tctcgaagtc attcgggaca acgtgagccg
     1981 gcagagcttc aagacctggt tcgcccccct caagggggtc aagctggacg agaccgagga
     2041 cggaaagaaa ctgaccgtcc agctgcccag ccgcttctac tacgagtggc tcgaggagca
     2101 ttactacggg ctcctgcgca agaccgtgac caaggtgctc ggtcccggcg gccgcctgtt
     2161 ctacgacatc gtcatcgaga aggacgacgc cgagtccgga tacgacggag cctcgatgca
     2221 ccttccggcg cgccggccgg cgaacgagcc ccagcccacc caggcgacgc tcgacgcgcc
     2281 ccggcccggc gcggcggtcg cccccttccc gtcccccggc ccgggcccgg tggccagccc
     2341 cttcgtgatc cccggcatcc agaagatcca cgtcgagagc cggctgaatc cgagctacac
     2401 gttcgaccgc ttcatcgagg gggactgcaa ccggctggcg cggtcggcct cgctcgccat
     2461 cgcccagcac ccgggtggaa cgagcttcaa cccgttcctg ctctacggcg gcgtgggcct
     2521 cggcaagacc cacctgatcc aggcgatcgg caactacatc gaggccaacc agtcgggggc
     2581 gaccacgctc tacacgtcgt cggagcgctt cacgaccgag ttcgtccagg cgatccagca
     2641 caaccgcgtc agtgatttct cgctcttcta tcgtcagatc gatctcctga tcatcgacga
     2701 cgtgcagttc ttcagcggca aggagaagac gcaggaggag ttcttccaca tcttcaacgc
     2761 cctgcaccag agcggcaagc agatcgtgct ctcggccgac cggccgccca aggacatcca
     2821 gggcatcgag gagcggctcc tctcccgctt ccagtggggc ctctcggccg acctgcagct
     2881 gcccgacctc gagacgcgga ccgccatcct gcagcgcaag tccgaggaag acggcatcga
     2941 cgtcgggccc gacgtcatcg agttcatcgc gcacaacgtg aagtcgaaca tccgcgagct
     3001 cgagggggcg ctgatccggc tgctcgccca ctccacgctc cacaagcggg agctggacct
     3061 gccgctcgcg cgcgatgtgc tgcgcgacct catcaaggag agccggatct cgctcacgat
     3121 cgaggagatc cagcgcctgg tctgcaccta cttctcgatc cccgaggacc tggtccgggc
     3181 caagacccgc aagcgcgagg tcgtgcaggc gaggcagatc gccatgttct tctccaagca
     3241 gttcacgcag cactcgctca agaccatcgg gctgcacttc ggcggacgcg accattccac
     3301 ggtcatccac gcgaaccaga gcgtcgagaa ccagatcgag acggatccca agttccggat
     3361 gatgatggac gagatccggc acaggatcga cctgcacagc cggtagccct cgggcgggat
     3421 tcttcgcggg cccgcggcgg ttcgaaccca cggtccgagt gcttaacttc ccgcgccgcc
     3481 cggcgccttc cgggctcccg catccgacgc acccttccgg ctgtacatca tgaagttcac
     3541 cgcggccagc tccgatctcc tcaaggcctt ccagaccgtc gccggggccg tacccacgaa
     3601 gagcacgctg cccatcctcg agtgcatcct cttcgaggcc gacggaaaca ccctgcggct
     3661 gagcgcgacc gacctggaga tctcgatccg gcagaagctc gccgtgacgc tcaaggggtc
     3721 gaacggtgcg ccgacccgcg tcgccgtgcc ggccaagcgc ctgctcgaca cgctgcgcgc
     3781 cctcccggag gttcccgtgg aattcgaggc cgacggcgac ttcaacgtgt ccctgcgcac
     3841 cgaccgcggc cgctacaaga tggtcggctt cgacggcgcg gacttcccgg cgctgcccga
     3901 cctgtcggag aagaccagga tcgagacctc gggagcgctg ctgcggcgcg cgatcacaaa
     3961 gacgagcttc gcggtgagcc gggacgcgct gcgcccggcg atgatgggca tctacttcca
     4021 gatcggctcc gacgaagggc gggccgtggc caccgacggg caccggctgg tgaagctgac
     4081 gctgcacgac atgaccgctc ccgagaacac ccacttcgtg gtgcccgaga aggccatgag
     4141 cctcgcagcg cgcgtcgcga ccgacgatcc gtgcggcatc acggtcgacg gcggctacgt
     4201 cgccttcgac ttcggccgct cgcgcgtcct tgcccggctc atcgacgagc cgtatccgaa
     4261 ctaccaggcg gtcatcccca tcgacaacga gaagacgctc gtcgtgtcgc gcgagtccat
     4321 gctcgcggcc gtcaagcgcg tggcgctcta ctcgagcacg atgaccaacc agatccgcct
     4381 gtccatctcg gcgaacgagg tgcgggtctc ggccgaggac atcgagcgcg ccagcgaggc
     4441 gcaggagacc gtagcgggcg agttccaggg ggaggacatg gtcatcggct tcaacgc
//
LOCUS       DBNHQZ010000276         2338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_82014, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000276 DBNHQZ010000000
VERSION     DBNHQZ010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_82014"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC16YU_07470"
     CDS             complement(<1..889)
                     /locus_tag="AC16YU_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6065100.1"
                     /translation="MLRITRFLLLPLLAAAVLWTGCDTTSDEESSGIVVLAGQVLNTQ
                     TNNPVAGAFVRVLPMDLLYEADSNGRYSIEVEIDSTMDLQVLASADGYSSTSVTVLAL
                     AGRTIQVPTLRIQQLVDGVTESGRAWSILLEGQSSGTIGVRESGSVEVANLTFTVVDS
                     VGRPVVVDQSAQVHFSFGAQPGGGESHAPLSAWTDNNGVVSVTLSSGTLAGVVQIMAE
                     TTVGTRVIRSQPVAVAIHGGLPDQDHFSVGPARFNFPGLIAYGLTNQVSVIVGDQYSN
                     PVRPGTSVYFSTTHGVIGGS"
     gene            1147..1461
                     /locus_tag="AC16YU_07475"
     CDS             1147..1461
                     /locus_tag="AC16YU_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6065101.1"
                     /translation="MAIRTVDMDMLANLTGNMYETVAILAKRARQVAMHTKAELDEKL
                     AYFEGFEQEIEDPRFQEEQARISLEYEVRPEASETAINEFLEGEIYYRDASSEELGDE
                     VF"
     gene            1551..2309
                     /locus_tag="AC16YU_07480"
     CDS             1551..2309
                     /locus_tag="AC16YU_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6065102.1"
                     /translation="MPRRRAPRVREFLPTIAWVWIAIALVTGGVLLRTAAPYGRHRRS
                     GWGPTVPHRAGWIVMELVSPVSLVAAATVVGGRLSPEASILAACWLGHYAWRAVVYPF
                     RARWGGRRMPVVVAAAAVLFNAVNGTLNGLALSWIAPTPGTLVAGLAVWAAGLAVNLH
                     SDGILRRLRAPGEHGYAVPHGGLFRWVSAPNYLGEIVEWAGFALAAWNPAALSFAVWT
                     AANLVPRALANHRWYRERFPEYPPERKALVPGLL"
BASE COUNT          383 a          761 c          838 g          356 t
ORIGIN      
        1 gcgagccccc gatgacgccg tgggtcgtgg agaagtacac ggacgtgccc ggccgcaccg
       61 gattcgagta ctggtcgccg acgatgacgc tgacctggtt cgtcaggccg taggcgatga
      121 gacccgggaa gttgaaccgg gccggtccca cgctgaagtg atcctggtcg ggaagtccgc
      181 cgtggatggc caccgcgacc ggctgcgagc ggatgacccg cgtgccgacc gtggtctcgg
      241 ccatgatctg gacgacgccg gccagggtgc cggacgagag cgtgacggac acgacgccgt
      301 tgttgtcggt ccaggcggag agcggcgcgt gggactcgcc gccgcccggc tgggcgccga
      361 acgagaagtg cacctgcgcg ctctggtcca ctaccacggg gcgtccgacg gagtcgacga
      421 ccgtgaaggt gagattggcc acctcgaccg agccgctctc ccgcacgccg atcgtgccgg
      481 aagactggcc ctcgaggagg atgctccagg cgcgtccgga ctcggtcacg ccgtcgacga
      541 gctgctggat gcgaagggtc ggcacctgga tggtccggcc cgcgagcgcg agcaccgtaa
      601 cggatgtcga ggagtatccg tcggcggacg ccaggacctg gaggtccatg gtgctgtcga
      661 tctcgacctc gatgctgtag cgaccgttgg agtcggcctc gtacaggagg tccatcggaa
      721 ggacgcgaac gaaggcgccg gcaaccgggt tgttggtctg agtgttcagg acctgcccgg
      781 cgaggacgac gatgccgctc gattcctcgt cggacgtcgt atcacagccc gtccagagga
      841 cggcagcggc gaggaggggc agaagaagga accgggtgat gcggagcatg gcggcaaggg
      901 gttgcgaaat gaggagtccg tgtcggtatc gtcgcgttcc aatcactcta aagcgaatct
      961 ccaacggcga acgccctacg ataggaggac acgcatccaa gcgaaaggcc ccgacatgcg
     1021 ccctgcgatg acgtcgcgtg aacaatgtgt gaagaggaaa ccggcagggg ctaggagcgc
     1081 attatagttc gctccgcaaa gttcaagcgg agcccgaccg ctcccatcac ccttcgcgag
     1141 accccaatgg cgatccgcac cgtcgacatg gacatgctgg ccaatctcac gggcaacatg
     1201 tacgaaaccg tcgcgatcct ggcgaagagg gcacggcagg tcgccatgca taccaaggcc
     1261 gagctcgacg agaagctggc ctacttcgag gggttcgagc aggagatcga ggatccccgg
     1321 ttccaggagg agcaggcccg catctccctc gagtacgaag tgcgccccga ggccagcgag
     1381 acggccatca acgagttcct cgagggcgag atctattacc gggacgcctc gtccgaggag
     1441 ctgggcgacg aggtcttctg accgaatctg caccggactt cgagggacgg acgccgcggc
     1501 gcccgtccct ttttcattga actcggcggg accgaccttg cgggcccgcc atgccccgac
     1561 gccgagcccc gagagtgcgc gaattcctac cgaccatcgc ctgggtctgg atcgcgatcg
     1621 ccctcgtgac gggcggcgtg ctgctcagga ccgcggcgcc gtacgggcgg caccgccgat
     1681 cgggatgggg tcccacggtg ccgcaccgcg ccggctggat cgtgatggaa ctcgtgtccc
     1741 cggtgtcgct ggtcgcggcc gcgacggtcg tcggggggcg gctttcgccg gaggcttcaa
     1801 tcctggccgc ctgctggctc gggcactacg cctggcgcgc cgtagtctac ccgttccgtg
     1861 cccgctgggg agggcgccgg atgccggtgg tggtcgcggc cgcggccgtg ttgttcaacg
     1921 ccgtcaacgg aacgctgaac gggctggcgc tctcgtggat cgccccgacg cccgggaccc
     1981 tggtggcggg cctcgcggtg tgggccgcgg ggctcgccgt gaacctgcat tcggacggaa
     2041 tcctgcgccg cctgcgcgcg cccggcgagc acggctacgc ggtgccgcat ggcggcctgt
     2101 tccgctgggt ctcggccccg aactacctgg gcgagatcgt cgagtgggcc gggttcgcgc
     2161 tggcagcctg gaaccccgcg gccctctcgt tcgccgtctg gacggcggcg aacctggtcc
     2221 ctcgggcgct cgcaaaccat cgttggtacc gggagcggtt ccccgaatac cccccggagc
     2281 ggaaggccct cgtcccgggc ctgctctgag gtcggcgcag acagccgcgc ggccgggt
//
LOCUS       DBNHQZ010000277         3157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3061513, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000277 DBNHQZ010000000
VERSION     DBNHQZ010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3061513"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="AC16YU_07485"
     CDS             complement(<1..549)
                     /locus_tag="AC16YU_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HIR6065103.1"
                     /translation="MIAEPRPGATVAPPDSAALVRVDPATPCAHCGLPVGRFPVGTDP
                     VFCCSACLAVYAALGESGLADTYYRLRDVGSVRPEAPARTDEDSGTLVELSSPGYLER
                     HTTLNEDGTRSAELFVDGVHCAACVWLVERMPIEMDGVHGARLDLARARLKLRWDPEV
                     VDLPSVSGWLSRYGYSAHPRRAD"
     gene            complement(553..1050)
                     /locus_tag="AC16YU_07490"
     CDS             complement(553..1050)
                     /locus_tag="AC16YU_07490"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR6065104.1"
                     /translation="MKDPILRPEIAWPAAIILLLLAGAGSAFAVLLFARSDGGAEVVA
                     DYYSRGLAWDSTAAVERSVERFGWTVSLEPAAGADSTGMRAVRIGIADSSGIPVGGLA
                     IDVEASRPHLATPAGRVAASPHLQPGVYVAWLPVREPGLWDFTVHGLRDGDPFALRLR
                     REILR"
     gene            complement(1055..2542)
                     /gene="ccoG"
                     /locus_tag="AC16YU_07495"
     CDS             complement(1055..2542)
                     /gene="ccoG"
                     /locus_tag="AC16YU_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR6065105.1"
                     /translation="MERDRDPQPAARRAAARVAESPEDVLATLTREGKRRWIYPTPSP
                     GRFLRRRTVVAWALVILYLALPLVPIGGRPAVLLDFVHREFALFGLVFYPTDTVLLLV
                     LLVGVLLSVILFTALLGRVWCGWACPQTVYLEFVFRPIERLIEGREHVRKRRDDGPLT
                     WAKAWRKGLKLSLYTAVSLVLAHTFVAYFVGWSALVDMMTGPPTENGLFFVMMAITTG
                     LVLFDFAYFREQMCTIACPYARMQSVLLDPDSLIVAYDTARGEPRGSRSRAALDREAA
                     MGSSVLGDCIDCTACVRTCPTGIDIRDGLQMECVACTQCIDACDVIMDRIGKPRGLIR
                     YTSEREAEGLPTRLVRPRTVIYGLLLALALAAFTTVLASREAYDVNVGRAVGEPFTVL
                     PDGRIANRIRFRVRNQTGSDTAFTVSARSDEDEHEDEDEARIEIRAVGLMPVPLGSGE
                     MERLETWVIADPDAFEHGVHEAEFEIAFDDGSTREVSFTLLGPKG"
     gene            complement(2578..>3157)
                     /locus_tag="AC16YU_07500"
     CDS             complement(2578..>3157)
                     /locus_tag="AC16YU_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6065106.1"
                     /translation="GPEQDVVIEGHNYDGIREYDNPMPGWWLGIFWASVVFAPIYMLG
                     VHVFGFIDTYEEDLAQGQAELQEIRSAHEAANPTFLADDATLAAYVGDQVHVEAGARL
                     FATNCAACHGNAGQGLIGPNLTDRYWIHGGRNADLFGVITNGVIEKGMTPWGSILSPE
                     QRAQLVAFIRSIEGTEPAGAREPQGELFEEGA"
BASE COUNT          505 a         1106 c         1108 g          438 t
ORIGIN      
        1 atcggcgcgc ctgggatggg ccgagtagcc ataccgggag agccatcccg acacggacgg
       61 caggtccacg acctccgggt cccatcgcag tttgaggcgc gcccgggcga gatccagcct
      121 ggcgccgtgc accccgtcca tttcgatcgg catgcgttcc acgagccaga cgcaggcggc
      181 gcagtgcacg ccgtccacga agagctcggc cgagcgggtg ccgtcctcgt tcagggtcgt
      241 gtgccgttcg aggtagccgg ggctcgacag ctcgacgagc gtcccggagt cttcatccgt
      301 gcgcgcgggt gcctccgggc ggacggatcc gacgtcacga aggcggtaat aggtgtccgc
      361 gaggccggac tcgccgagcg cggcgtacac cgccaggcac gcgctgcagc agaacacggg
      421 atccgtgccg accgggaagc ggccgaccgg caggccgcag tgcgcgcagg gcgtcgccgg
      481 atcgacacgg acaagtgccg cggaatcggg cggcgcgacg gtcgcgccgg gacgcggctc
      541 cgcgatcatg gctcagcgca ggatctcccg ccgcagccgg agcgcgaacg ggtccccgtc
      601 ccgaaggcca tgcaccgtga agtcccacag tcccggctcg cgcaccggga gccaggccac
      661 gtacacgccg ggctgcaggt gcgggctcgc cgccacgcgg cccgcggggg tcgcgagatg
      721 cggcctcgac gcctcgacgt cgatcgccag gccgccgacc ggaatccccg aggagtccgc
      781 gattccgatc cggacggcgc gcatcccggt gctgtccgcc ccggcggccg gctcgaggct
      841 cacggtccag ccgaagcgct ccaccgagcg ctccacggca gccgtcgagt cccacgcgag
      901 gccgcggctg taatagtccg caaccacctc ggcgccaccg tccgaccggg cgaagaggag
      961 caccgcgaaa gcggatccgg cccccgcgag aagcagcagg atgatcgcgg ccggccaggc
     1021 gatctcgggc cggaggatcg ggtctttcat gtgctcagcc cttgggtccg agaagggtga
     1081 aggatacctc ccgggtcgat ccgtcgtcga acgcgatctc gaattcggcc tcgtgaacgc
     1141 cgtgctcgaa ggcatccgga tcggcgatca cccaggtctc caggcgctcc atctccccgc
     1201 tgccaagcgg cacgggcatg aggccgaccg cgcggatctc gatcctggcc tcgtcctcat
     1261 cttcgtgctc gtcttcgtcc gacctggccg atacggtgaa ggccgtgtcg gatcccgtct
     1321 ggttccgcac gcggaagcgg atccggttcg cgatgcggcc atccggaagc acggtgaatg
     1381 gctcgccaac ggcccggccc acgttcacgt cgtacgcctc gcggctggcg aggaccgtgg
     1441 tgaaggccgc gagcgccagg gcgagcagca gcccgtagat cacggtgcgg gggcgcacca
     1501 gtcgggtggg caggccctcc gcctcgcgct ccgacgtgta gcggatcagc ccccggggct
     1561 tgccgatccg gtccatgatg acgtcgcagg cgtcgatgca ctgcgtgcag gccacgcact
     1621 ccatctgcag gccgtcccgg atgtcgatgc cggtcgggca cgtccgcacg caggccgtgc
     1681 agtcgatgca gtcgcccagg acggacgatc ccatggccgc ctcccgatcc agcgccgcgc
     1741 gcgatcggct tccccgcggc tcgcccctgg ccgtgtcgta ggccacgatc agggaatcgg
     1801 gatcgagcag caccgactgc atgcgcgcgt acgggcaggc gatcgtgcac atctgctcgc
     1861 ggaaatacgc gaagtcgaag aggacgagac cggtcgtgat ggccatcatc acgaaaaaga
     1921 gaccgttctc ggtgggcggc ccggtcatca tgtcgacgag cgcggaccag cccacgaaat
     1981 aggccacgaa cgtgtgcgcc aggaccagcg agacggccgt gtacagggag agcttgagcc
     2041 ccttgcgcca ggccttggcc cacgtcagcg gtccgtcgtc acgccgcttc cggacgtgtt
     2101 ccctgccctc gatgaggcgc tcgatggggc ggaacacgaa ctcgaggtac acggtctgcg
     2161 ggcaggccca gccgcaccag acccggccga gcagcgccgt gaacaggatg accgaaagca
     2221 gcacgccgac caggaggacc aggagcagca ccgtgtcggt gggatagaag acgagcccga
     2281 acagcgcgaa ttcccggtgc acgaaatcga gcaggacggc gggccggccc ccgatcggga
     2341 ccagggggag cgcgaggtac aggatgacca gggcccaggc gaccacggtc cgccgccgca
     2401 ggaatcgccc cgggctcggc gtcgggtaga tccagcggcg cttgccttcg cgcgtgagcg
     2461 tggccaggac gtcttccggc gattccgcta cccgggcggc cgcccggcgc gccgcaggct
     2521 gcggatcacg gtctctttcc atggggttcg gtgcgaccgg ggtgtgcgcc cggccggtca
     2581 ggctccttcc tcgaacagct cgccctgcgg ctcgcgtgcg cccgcgggct cggtgccctc
     2641 gatcgaccgg atgaacgcga cgagctgggc gcgctgctcc ggcgacagga tgcttcccca
     2701 cggggtcatg cccttctcga tgacgccgtt cgtgatgacg ccgaagagat ccgcgttccg
     2761 gccgccgtga atccagtagc ggtcggtcag gttcggaccg atcaggccct gcccggcgtt
     2821 gccgtggcag gcggcgcagt tcgtggcgaa gaggcgggca cccgcctcga cgtgcacctg
     2881 gtctccgacg taggcggcga gggtcgcatc gtccgccagg aacgtggggt tggccgcctc
     2941 gtgggccgac cggatctcct gcagctccgc ctggccctgg gccaggtcct cctcgtacgt
     3001 gtcgatgaag ccgaacacgt ggacacccag catgtagatc ggggcgaaca ccacggacgc
     3061 ccagaaaatg cccagccacc agcccggcat cgggttgtcg tattcgcgga tcccgtcgta
     3121 gttgtgtccc tcgatgacga cgtcctgctc cgggccg
//
LOCUS       DBNHQZ010000278         8576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_624358, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000278 DBNHQZ010000000
VERSION     DBNHQZ010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_624358"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC16YU_07505"
     CDS             complement(<1..357)
                     /locus_tag="AC16YU_07505"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065107.1"
                     /translation="MSEDTGRGPLIAARGLTRVYEDRNSVPVHALRGVDLDIHAGEFT
                     AVAGPSGSGKTTLLNLLSGLDRPTAGRVTIAGKDITELSRGELADFRLHNVGFVFQAF
                     NLIPVLSAAENAEFTLL"
     gene            complement(350..1570)
                     /locus_tag="AC16YU_07510"
     CDS             complement(350..1570)
                     /locus_tag="AC16YU_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065108.1"
                     /translation="MLLTIAWRNVGRNRRRSAVLVGAVAFGVLAYIGSTAFMDGLGIE
                     LVAGAVDLQGGHVQVAASGYHGNPTIESRIADPDAVLAAVARVPDASAAPEVVTPGMA
                     NSTEQSGGIVIHGVDPVLEPGVTAIAERVVEGSWLDAFGAADQVVVGHGLAERLRVGL
                     GEKIVLMASDLDNEVSAGAYRISGLYRSGSTPFDKTNVYILLGEAQRMLGFDGEVSTV
                     TVRLALPDEAVPVARSLSDELSGRDLEALSWEERNPMIEMAKEAYDYSAVITALILFA
                     AVGFTLVNSFLMVIFERIHELGIMLAQGVKPAYIRRMLFLEAVIVAAIGAVVGVVLAT
                     AVLAYWIHAGMDLSSFAEGLGALSVRTVIYPYLDWGHLGMGFGVIAVMVALSVLYPAI
                     KASRFEAVEAINHV"
     gene            complement(1581..2819)
                     /locus_tag="AC16YU_07515"
     CDS             complement(1581..2819)
                     /locus_tag="AC16YU_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065109.1"
                     /translation="MRENLILAWRNVWRNRRRSLITISAVVFSVLIITLTRSLQYGSY
                     DAMEASAVQMYTGEIQIQRPGYREEQTLEYALDGDEEAWRVALEGAPWLEATARRLTG
                     FGLVSSDESSAGAMIVGVEPEAERRVSKFSDRVTLGLPLGGDSDHEALLGQILARNLR
                     VEVGDTVVVLTQGWHNQMGADLYSVRGLLRTGSVEMDRAIMILPLHDAQFLFSMGDRF
                     TEVVLRTDDFRLARSYAESIRGKLPDDRFSVYDWDALMPELRQMIVLDNVSGAIYLLF
                     LLVLVGFEIFNTTTMSVMERIREFGVLQALGMRPQRIARLVMAELAVKVAVALAVSIG
                     ASGLLLWYLEGNPIPMPAEFVEMYEEFGFSVESLVFSTRPAVFLEPLLAVAVIAVLAT
                     IYPVLRVLNLTTIDALRRGT"
     gene            complement(2831..3589)
                     /locus_tag="AC16YU_07520"
     CDS             complement(2831..3589)
                     /locus_tag="AC16YU_07520"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6065110.1"
                     /translation="MRAAFPYLLCLSVLPLVARPAGGQPTAREIVSRADSLTRGDTQQ
                     GRYEMRIVRPDWERSTTFEFWSEGTEKAFIRVVEPARDRGVAFLKVGREMWNYIPRVN
                     RVIKIPPSMMLQSWMGSDFTNDDLVKESSVVDDYEHTLIGSDTLRGADVWHVELVPKD
                     GTAVAWDRIQEWIRKEDYVPLKAVYFNERGEITRTMLFTGIRRMGGRLLPTRYDLIEE
                     TRPGRRTVLILEDVVFDRPVDHRVFSQQNLRRGS"
     gene            3742..4563
                     /locus_tag="AC16YU_07525"
     CDS             3742..4563
                     /locus_tag="AC16YU_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6065111.1"
                     /translation="MNHSDRMTRLEHVGIAAVSADAVAAVFEALFGRRPYAREFVGRE
                     GVRTTFIEAGPVKLELLEATGPDSAMARSVERRGPGLHHLAFEVENLDAAHARLEAAG
                     FRLLGDPAPGADRKRIFFIHPKDTAGVLVECCESVPGGATALSGSGPEGAPPCLVIAD
                     EGEWDVVRSLARRMRVWHATGGARAPGAPPESHLVVFGAHSDAVAGAPAGLVLVDPLP
                     EAVTHARPDALVISRDGRDLPQRPGTLVRLPHGLLARLPDPAATLADVIVAHLIA"
     gene            4773..6101
                     /locus_tag="AC16YU_07530"
     CDS             4773..6101
                     /locus_tag="AC16YU_07530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR6065112.1"
                     /translation="MKPSTPLGLSRLAAAALLAVAFSGCADSFTGDAADSTPALVDSE
                     SASSPKLRHAHLLGDAVRPSASADVASKKGQSLSLVIGLDEQRILERYRILERYRILE
                     RYRFTESFLGIAITVDDLTGESDFRALIDALIEDDEVAWIEPDFRISGPVARNAANQP
                     GQVVPWNVAMVGGESSSAVSGDGTGTVPVDVYVIDSGITSDDVRVVDAIDFRGTDDPS
                     DGDGHGTHVAGTIAAVDDEDGVVGIAPGANVHNLRVLDENGSADVSVVIAAMEHVIAA
                     RRAQPTRPMVVNLSIGENIGSADFTALDLAVDQAVSMGIVVVVAAGNQGVDATLVTPA
                     HVSSALTVGAFDAAGGFAPFSNHGPFVDLLAPGVDVVSIQPGDRDRKARMSGTSMASP
                     HVAGAAALYLSSHPEATPDEVRQALIDASKGPVHFVPQGTTSRSVWVGSF"
     gene            complement(6108..6629)
                     /locus_tag="AC16YU_07535"
     CDS             complement(6108..6629)
                     /locus_tag="AC16YU_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6065113.1"
                     /translation="MLRITVTGAECTGKTTLVRGLAERFGAPWVPEAARLFVERARRP
                     VRRTDVEAIARLHLELLDAAVESGAPLVLCDTDLLSTVAYARHYFGSCPRAVTRRAFE
                     RPADLYLLAADDLPWIADPHQRGTEEDRSRVQARLRSLVRRTGRPSVEVGGGVSGRLE
                     AATRAIDRFRQSG"
     gene            6725..7696
                     /locus_tag="AC16YU_07540"
     CDS             6725..7696
                     /locus_tag="AC16YU_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR6065114.1"
                     /translation="MLNYIWAGLIGFSLVFAMVYDVRDLARDTYRNGEPVPVRIEFPE
                     GYLQTARSVPVVVRFTDFAERYGVDAPDPGPLEGTLVQGADGRELRFGRDTALPEPLA
                     TIRQMTSERDNDLRGPVPAFEVTGTAGATATVRFARVRFVKLQAIADAAFGMAETAVT
                     IALGLIGVIALWMGLLKIAETSGLINVVVRLAQPVLRPLFPEIPRDHPALGLIVLNLS
                     ANMLGLGNAATPLGIKAMESLQELNTDKDTATNSMVMLLAMNTASVQLVPPVLLVAIM
                     GLQINKLIFAILIVTAISLVVAIVAAKLLGRMPGYRKTDPNRPTETV"
     gene            7702..8244
                     /locus_tag="AC16YU_07545"
     CDS             7702..8244
                     /locus_tag="AC16YU_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIR6065115.1"
                     /translation="METLRSVIDVLSVFVLPSLIVGFPLYGLYKKVPVYEEFVTGAKE
                     GFGVAVMIIPYLVAILFAIGMFRASGAMEFVVDGMRPVLSLIGVPSEVLPMMIIRPLT
                     GSGSAAVVLDLIGQFGEDSIIVKIAATMFGSTETTFYVIAVYFGAVNVRKTRHAVPAG
                     LIADGVALLLAVYVVQWLFG"
     gene            complement(8307..>8576)
                     /locus_tag="AC16YU_07550"
     CDS             complement(8307..>8576)
                     /locus_tag="AC16YU_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR6065116.1"
                     /translation="KTVRVTFSLPADAATKAVAVVGEFNGWDAGKGIMKLDRKAGAWT
                     KGVSLKPGASYQFRYLVDGKSWRNDEAADAYVPNEFFSENGVLEL"
BASE COUNT         1353 a         3038 c         2830 g         1355 t
ORIGIN      
        1 gagcagggtg aactccgcgt tctcggcggc cgacaggacg gggatcaggt tgaaggcctg
       61 gaatacgaac cccacgttgt gcaggcggaa atcggcaagc tccccccggg aaagctccgt
      121 gatgtccttc ccggctatcg tcacccgccc cgcggtgggg cgatcgagcc cgctcaggag
      181 gttcaggagc gtggtcttgc ccgatcccga gggacccgcg acggcggtga actccccggc
      241 gtggatgtcg agatccacgc cccgcagcgc gtgcacgggc acgctgttgc gatcctcgta
      301 gaccctcgtg aggccgcgcg ccgcgatcag gggtcccctg cccgtgtctt cagacatggt
      361 tgattgcctc caccgcctcg aagcggctcg ccttgatcgc cggatacaga acggacagcg
      421 ccaccatcac ggcgatgacc ccgaatccca ttccgaggtg cccccagtcc aggtacgggt
      481 agatgaccgt gcgcacgctc aacgcgccca ggccctccgc gaagctgctc aggtccatgc
      541 ccgcgtgaat ccagtacgcg aggaccgccg tcgcgagcac gacgcccacc acagcaccga
      601 tcgccgcgac gatcacggcc tccaggaaga gcatccgtcg gatgtaggcc ggcttcacgc
      661 cctgcgcgag catgattccc agctcatgga tcctctcgaa gatgaccatg aggaacgagt
      721 tcaccagcgt gaatcccacg gccgcgaaca ggatgagcgc cgtgatgacc gccgagtaat
      781 cgtaggcctc cttggccatc tcgatcatcg ggttgcgctc ctcccaggag agggcctcga
      841 gatcgcgtcc cgagagctcg tccgaaaggc ttcgggcgac ggggacggcc tcgtccggaa
      901 gcgccagccg gaccgtcacc gtggacacct cgccgtcgaa gcccagcatc cgctgggcct
      961 cgccgagcag gatgtacacg ttggtcttgt cgaagggcgt gctgcctgac cggtacaggc
     1021 cggagatccg gtatgcgccg gcgctcacct cgttgtccag gtcgctggcc atgagcacga
     1081 tcttctcgcc cagccctact cgcagccgct cggcgaggcc gtggcccacg accacctggt
     1141 cggcagcccc gaaggcgtcg agccacgatc cctcgacgac ccgctccgca atggcggtga
     1201 cgccgggctc cagtaccggg tccacgccgt gaatcacgat cccgcccgac tgctcggtgg
     1261 agttcgccat gccgggcgtg accacctccg gggccgcgga ggcgtccggg acgcgggcga
     1321 cggcggcaag aaccgcatcc ggatcggcga tccgtgactc gatggtcggg ttgccgtggt
     1381 accccgaggc cgccacctgg acatgcccgc cctgcaggtc cacagctccc gctacgagtt
     1441 cgatgccgag gccgtccatg aaggcggtcg acccgatgta cgcgagcacc ccgaacgcga
     1501 cggcgccgac caggacggcc gagcggcggc ggttcctgcc cacgtttcgc caggctatgg
     1561 tgaggagcat ccgcatcggg tcaggtgccc cgtcggaggg cgtctatggt ggtcaggttc
     1621 agtaccctga gcacggggta gatcgtggcg agcacggcga tgaccgccac ggcaaggagc
     1681 ggctccagga acaccgccgg acgcgtcgag aagacgaggc tctcgacgga gaatccgaac
     1741 tcctcgtaca tctcgacgaa ctcggcgggc atgggaatgg gattcccctc gagataccag
     1801 agcagcaacc cgctcgcgcc gatcgacacg gcgagggcca ccgccacctt caccgcgagc
     1861 tccgccatca cgaggcgggc gatgcgctgc ggccgcatgc ccagggcctg gagcaccccg
     1921 aactcccgga tcctctccat gacgctcatt gtggtcgtat tgaagatctc gaagcccacg
     1981 aggacgagca gaaagagcag gtagatggct ccgctcacgt tgtcgagcac gatcatctgg
     2041 cgcagctccg gcatgagcgc gtcccagtcg tacacgctga agcggtcgtc cgggagcttc
     2101 ccgcggatcg actcggcgta cgagcgcgcc aggcggaaat cgtcggtcct gagcacgacc
     2161 tcggtgaagc ggtcgcccat cgagaacagg aattgggcgt cgtgcaacgg gaggatcatg
     2221 attgcgcggt ccatctcgac gctgccggtc cgcagcaggc cccggacgct gtacaggtcg
     2281 gcacccatct ggttgtgcca cccctgggtg agaaccacga ccgtgtctcc cacttccacg
     2341 cgcaggttcc gggcgagaat ctgcccgaga agcgcctcgt gatcactgtc acccccgagc
     2401 ggcaggccga gcgtcacgcg gtccgagaac ttgctcaccc gccgctcggc ctccggttcg
     2461 acgcccacga tcatggctcc cgcggaggac tcgtccgagc tgaccaggcc gaagcccgtg
     2521 agacggcgcg cggtggcctc gagccacggc gccccctcca gtgcgaccct ccacgcctcc
     2581 tcgtcgccgt ccagcgcgta ctcaagcgtc tgctcctccc tgtaaccggg gcgctggatc
     2641 tggatctcgc ccgtgtacat ctggacggcc gaggcctcca tggcatcgta cgagccgtac
     2701 tgcagcgagc gggtgagcgt gatgatgagc acgctgaaca ccacggccga gatcgttatc
     2761 aggctccggc gccggttgcg ccacacgttc cgccaggcca ggatcaggtt ctcacgcatg
     2821 gtccgggccg tcaggatccg cggcggagat tctgctgcga aaagacgcga tggtccaccg
     2881 gccggtcgaa gaccacatcc tcgagaatca gaacggtccg gcgcccggga cgcgtctcct
     2941 cgatcaggtc atagcgggtc ggcaggagcc ttccgcccat ccgccggatc cccgtgaaaa
     3001 gcatcgtccg ggtgatctcg ccccgctcgt tgaagtagac cgccttgagc gggacgtagt
     3061 cctccttccg gatccattcc tggatgcgat cccaggccac ggcggtcccg tccttcggca
     3121 cgagctccac gtgccacacg tccgcgccgc ggagcgtgtc cgacccgatg agcgtgtgct
     3181 cgtagtcgtc gaccacgctc gactccttga ccaggtcgtc gttcgtgaag tcggacccca
     3241 tccacgactg cagcatcatc gacgggggaa tcttgataac gcggttgacc ctcgggatgt
     3301 agttccacat ttcccggccc accttcagga aggccacccc cctgtcgcgg gccggctcga
     3361 ccacgcggat gaacgccttc tcggtgccct ccgaccagaa ctcgaacgtc gtggagcgct
     3421 cccagtccgg ccgcacgatc cgcatctcgt aacgtccctg ctgcgtgtcc ccgcgggtga
     3481 ggctgtcggc ccgcgaaacg atctcgcgcg ccgtcggctg gccgccggcc ggccgcgcga
     3541 cgagtggcag gaccgaaagg cacagaaggt acgggaaggc cgcgcgcatg cgccgagctc
     3601 gacgggttgt gccgattgta cgatccacgg ggcaaaggtt ggccccgccg cgtgggggaa
     3661 tcccggaacc tatcgtgggg ctcggtcccc ttgcttccgt gcggcgcatg tcctattcag
     3721 caccgaaggt ccccgttccc catgaaccac agcgaccgca tgaccaggct cgagcacgtc
     3781 ggtatcgccg ccgtcagcgc cgacgcggtg gccgcggtgt tcgaagccct gttcgggaga
     3841 cggccctacg cccgggagtt cgtggggcgg gaaggtgtcc gcacgacgtt catcgaggcg
     3901 ggtccggtga agctggagct gctggaggcc acgggccccg actccgccat ggcccggtcc
     3961 gtggagcggc gcgggcccgg tctgcatcac ctggccttcg aagtggagaa cctcgacgcc
     4021 gcccacgcgc gactggaagc ggcaggtttc cgcctgctcg gagatccggc cccaggggcc
     4081 gaccggaagc ggatcttctt catccatccg aaggacacgg ccggcgtcct ggtggaatgc
     4141 tgcgagtcgg tgccgggtgg ggcgacggcg ctctccggtt cgggacccga gggggctccg
     4201 ccctgcctcg tgatcgcgga cgagggcgaa tgggacgtgg tccgctcgct cgcgcgccgg
     4261 atgcgcgtgt ggcatgcgac gggcggtgcc cgcgcacccg gtgcgccgcc cgagagccat
     4321 ctcgtggttt tcggggcgca ttccgacgcc gtggccggag cgccggccgg cctggtcctg
     4381 gtggatcccc tccctgaagc cgtcacgcac gctcgcccgg acgcgctcgt catctcgagg
     4441 gacgggcggg acctgccaca gcgcccgggc acgctcgtgc gactcccgca cggactcctc
     4501 gcccggctgc ccgatcctgc ggccacgctc gcggatgtca ttgtggcaca tctgatcgcc
     4561 tgagggcggt ccgggcggcg gtcgaagcgt caggccgcca cgaagtggac gggatgcgct
     4621 taaggagcgc ctccgacagc cgataccccg agggcccgaa gtgcgactgc gcagtgcggg
     4681 gtcacaggcc cccgctgcag gccgttacga cgggcggggt cggtccctcc aaacgggacc
     4741 gcccctacca caacggatcc gtcaaggctg tcatgaaacc gtccacgccc ctcggcctgt
     4801 cccgcctggc ggcggccgct ctcctcgcgg tcgccttctc cggctgtgca gactcgttca
     4861 ccggcgacgc cgcagactcc actccggccc tcgtcgattc ggaaagcgcc tcctcgccca
     4921 agctccgtca tgcccacctt ctgggcgacg ccgtgcggcc gtccgcgtcc gccgacgtgg
     4981 catcgaagaa gggccagagc ctctcgctcg tgatcgggct ggacgaacag cggattctcg
     5041 aacgctaccg catcctcgag cgctaccgca tcctcgagcg ctaccgcttc accgagtcct
     5101 tcctgggcat cgccatcacg gtcgacgacc tcacgggcga gtccgacttc cgcgccctga
     5161 tcgatgccct gatcgaggac gacgaggtgg cctggatcga gcccgacttc cggatctcgg
     5221 gccccgtggc ccgcaacgcc gcgaaccagc ccggccaggt ggtcccgtgg aacgtggcga
     5281 tggtgggcgg cgaatcgagc tccgcggtgt cgggtgacgg cacggggacg gtccccgtgg
     5341 acgtatacgt gatcgactcc ggcatcacga gcgacgacgt ccgcgtcgtc gatgccatcg
     5401 acttccgcgg cacggacgat ccgtccgacg gggacgggca cggcacgcac gtggccggca
     5461 ccatcgcagc ggtcgacgat gaggacggcg tcgtgggcat cgcccccggt gccaacgtgc
     5521 acaacctccg ggtgctcgac gagaacggat ccgcggacgt gtccgtggtc atcgccgcca
     5581 tggagcacgt gatcgccgcg cggcgcgccc agcccacgcg gccgatggtc gtcaatctct
     5641 ccatcggcga gaacatcggg tcggccgatt tcacggccct cgacctcgcc gtcgaccagg
     5701 ccgtgtcgat ggggatcgtc gtcgtcgtgg cggccggaaa ccagggcgtg gacgccacgc
     5761 tcgtgacccc tgcgcacgtg tcgtcggccc tgacggtagg tgcattcgac gcggcgggcg
     5821 gctttgcccc gttctcgaac cacggcccct tcgtggacct cctcgccccg ggggtggacg
     5881 tcgtgtcgat ccagccgggc gacagggaca ggaaggcccg catgagcggc acctcgatgg
     5941 cgtctcccca cgtggccggg gccgcggcgc tgtacctgtc aagccatccg gaggccacgc
     6001 ccgacgaggt ccggcaggcc ctcatcgacg cctcgaaagg gccggtccat ttcgtgccgc
     6061 aggggacgac gagccggtcg gtctgggtcg gcagcttctg agccccgtca gccggactgc
     6121 ctgaaccggt cgatcgcccg cgtcgccgcc tccaaccggc ccgagacgcc tccgccgacc
     6181 tcgaccgagg gccgacccgt ccggcggacg agggaacgca gtcgagcctg gacccgggag
     6241 cggtcctctt ccgtgccccg ctggtgcgga tccgcgatcc acgggaggtc gtccgcggcc
     6301 aggaggtaca ggtcggcggg acgctcgaac gcccggcgcg tgaccgcgcg cggacacgac
     6361 ccgaagtagt gcctcgcgta ggctaccgtg gacagcaggt ccgtatcgca caggaccagc
     6421 ggcgcgccgg actccaccgc cgcgtccagg agctcgaggt gcagccgggc gatcgcctcc
     6481 acgtccgtgc gccggacggg gcgccgggcc cgttccacga acaggcgggc cgcttcgggc
     6541 acccacggcg cgccgaagcg ctcggccagc ccgcgcacga gggtcgtctt tcccgtgcac
     6601 tcggctcccg tcaccgtgat tctgagcacc ggtcctcctg ctcggttgcg gctcggactc
     6661 caacgccgat gcgcgggtgc gcgttcattt cctatcatga ccgccccgga agccggacga
     6721 tcccatgctg aactacatct gggcgggcct gatcggattc agcctcgtct ttgccatggt
     6781 gtacgacgtg cgcgacctcg cacgggacac ctaccggaac ggagagcccg tgccggtccg
     6841 gatcgagttc ccggagggat acctccagac ggcccgctcc gtgcccgtcg tcgtccgctt
     6901 cacggacttc gccgagcggt acggcgtcga cgccccggac cccggaccgc tcgaaggaac
     6961 gctcgtgcag ggtgccgacg gacgcgaact gcgcttcgga cgggacaccg cgctccccga
     7021 gccgctcgcc acgatccggc agatgacctc cgagcgtgac aacgacctcc ggggaccggt
     7081 ccccgctttc gaggtgacgg gcaccgccgg ggcgaccgcc accgtgaggt tcgcccgggt
     7141 ccgattcgtg aagctccagg cgatcgccga cgccgcgttc ggcatggccg aaacggccgt
     7201 gacgattgca ctcggcctca tcggggtcat cgccctgtgg atggggctgc tgaagatcgc
     7261 cgagacgtcc gggctcataa acgtcgtggt gcgcctggcc caacccgtgc tgcggccact
     7321 cttccccgag atcccgcgcg atcacccggc cctcggactc atcgtgctga acctgtcggc
     7381 gaacatgctc ggcctgggca acgccgcgac cccgctcggc atcaaggcga tggaatcgct
     7441 ccaggagctg aacaccgaca aggacacggc cacgaactcg atggttatgc tcctggcgat
     7501 gaacacggcg tcggtgcagc tcgtcccgcc ggtgcttctg gtcgcgatca tgggcctgca
     7561 gatcaacaag ctgatcttcg cgatcctgat cgtgacggcg atctcgctcg tcgtggccat
     7621 cgttgcggcg aagctgctcg gtcgcatgcc gggctatcgc aagaccgacc ccaaccgtcc
     7681 gaccgaaacc gtctgaaggc catggaaacc ctgcgctccg tcatcgacgt cctctcggtc
     7741 ttcgtcctac cgtcgctgat cgtcggcttt ccgctctacg gcctctacaa gaaggtcccc
     7801 gtgtacgagg agttcgtcac cggtgccaag gagggattcg gcgtcgcggt gatgatcatt
     7861 ccctacctgg tggccatcct gttcgcgatc ggcatgttcc gggcgtcggg ggcgatggaa
     7921 ttcgtggtcg acggcatgcg gccggtgctg tcgctcatcg gggtgccgtc cgaggtgctg
     7981 ccgatgatga tcatccgccc gctcacgggg tccgggtcgg ccgccgtggt gctggacctc
     8041 atcggccagt tcggggagga ctcgatcatc gtgaagatcg cggcgaccat gttcggctcc
     8101 accgagacga ccttctacgt cattgccgtg tatttcggcg ccgtgaacgt ccgcaagacc
     8161 cggcacgccg tgcccgccgg actcatcgcg gacggggtgg cgctgctgct ggcggtctac
     8221 gtcgtgcagt ggctcttcgg ctgacccgga aacggaaaag ggagacaccg gacggcgcct
     8281 ccccttcgtc tcgacgctcc cggacgtcag agctccagga cgccgttctc gctgaagaac
     8341 tcgttcggga cgtacgcgtc ggcggcctcg tcgttgcgcc agctcttgcc gtccacgagg
     8401 taacggaact ggtaggacgc accgggcttc agcgagacgc ccttggtcca ggcaccggcc
     8461 ttccggtcca gcttcatgat gccctttccg gcatcccatc cgttgaattc gccaacgacg
     8521 gcgaccgcct tggtagcggc atcggccggc agggagaagg tcacccgcac ggtctt
//
LOCUS       DBNHQZ010000279         2510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3062168, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000279 DBNHQZ010000000
VERSION     DBNHQZ010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3062168"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1861
                     /locus_tag="AC16YU_07555"
     CDS             332..1861
                     /locus_tag="AC16YU_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065117.1"
                     /translation="MAGPSRHSLRSARSILRWWISLVVSGLPLAAAGQVRPDPLGGAD
                     HVALGVYGETAWWLEGARLVGLDLRSGEARSRDLSDAFRVFEPWEANVGVDPNPPAWY
                     SSAIDARRGVLWLWDRSVGATVRVGLADADTMVVATARRSSLTQFDHAGGLHPMTGLP
                     LAYGGFGYHRSVEFAVTYDELAPTWLEIAYRGSERPAARLGATMIDGWHPTRIAIVGG
                     FAASDDTPPFTYDRPLADAWTYDFADSTWSEIDLPPAVRCAIESSAPRLGDVAADPVT
                     HGVLLYRPASCSSSGSGAILRWFPDRGDVQLVGELPDSAPEAVPFALYYPPDSASARM
                     VFQTPELGRPRAEWSRLSFDLPLPPQPAPGSGWPLAAVPVALGALLLGTWEWVRRRRR
                     NGREVVVSLNGDDRITLRRNGDALDRRASDNARHVLESLAATGILAGDTVSADQVSQI
                     LDSLYTDPDSLRVARNRAVDEVNALFLEAFGADLLERVRRSDDARRFDWRVRWTVRME
                     G"
     gene            1975..>2510
                     /locus_tag="AC16YU_07560"
     CDS             1975..>2510
                     /locus_tag="AC16YU_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6065118.1"
                     /translation="MTLTSSLRTGLALALGLVLLAGCSPDSGSAQVAATPDMNTPVVA
                     AAASERAQRILENLKYEIPQLRQLPVIMGDVLDGTVAGLDRGTFMVQGQTYHYIVTED
                     DSQLFMLAAPAIDASRSADDLAAAYEEEQAAEARAAEETASALAEASSTLPSLGPADA
                     PVTIVEFSDFQCPFCRRAL"
BASE COUNT          357 a          914 c          838 g          401 t
ORIGIN      
        1 cggccggcac gcgccgaacg ccaaggcggc aacgacgagc cacccggagg cgcggcgagg
       61 cgtttcgcga ccgtccgacg acatctggcc ttcccgtttc gaatcaggtc cgaagcaccg
      121 acgaagctat aaccccgtct ccgacgaagc aggagaagtt tcgattctga ttcgaaacga
      181 gtcctgtaag tccgggtact ccccggcagg tggacaggga cgaaccgggg cgcgcatctt
      241 gccccggtcc attttcattt gtccgaatcg gaccgatttt ccgggagtga gccgggcggc
      301 ggccgaccgg accgtcccgc ccatccgtcc catggccggc ccgtccagac atagcttgcg
      361 ctcggcgcgg tccatcctcc gctggtggat cagcctcgtc gtgtcgggcc tgccgctcgc
      421 cgcggccggc caggtccgtc cagatccact cggcggcgcg gatcacgtgg ccctgggcgt
      481 gtacggggag accgcctggt ggctggaggg cgcgcgtctc gttggcctcg atctgcgatc
      541 gggcgaggcc cggagccgcg acctgtcgga cgccttccgg gtcttcgagc cgtgggaggc
      601 gaacgttggc gtcgatccga atccgcccgc ctggtattcc agcgccatcg acgcgcggcg
      661 cggcgtcctc tggctctggg atcggagcgt gggggcgacg gtacgggtcg gactggcgga
      721 tgccgacacg atggtggttg cgacagcccg gcggagcagt ctcacgcagt tcgaccacgc
      781 gggcggtctc caccccatga ccggccttcc gctcgcgtac gggggcttcg gctaccaccg
      841 gagcgtcgag ttcgcggtaa cctacgatga actcgctccc acgtggctcg agatcgcgta
      901 ccggggctcg gagcgcccgg ccgcccgact cggcgccacg atgattgacg gctggcatcc
      961 cacccgcatc gcgatcgtgg gcggattcgc agcctcggac gacacgccac cctttacgta
     1021 cgaccggccg ctcgccgacg cgtggacata cgacttcgcg gacagcacat ggagcgagat
     1081 cgacctgccg cccgccgtcc ggtgcgcgat cgagtcgtct gcaccgcggc tgggggacgt
     1141 cgctgccgat cccgtcacgc acggcgtgct gctctaccgg ccggcctcgt gctccagcag
     1201 cggttcaggc gccattctcc gatggttccc tgaccggggc gacgttcaac tggtgggcga
     1261 gcttcccgat tcggcgcccg aagccgtgcc gttcgcgctc tattatccac ccgattcggc
     1321 ctcggcccgc atggtgttcc agacgcctga actgggccgt ccgcgtgccg agtggagccg
     1381 attgagcttc gacctgccgc tcccgccgca accagcgccg ggctccgggt ggcccctggc
     1441 cgcggtcccg gtcgctctcg gcgctcttct cctgggaacg tgggaatggg tgcgccggag
     1501 aaggcgaaac ggccgcgaag tcgtggtctc cctgaacggc gacgaccgga tcaccctccg
     1561 caggaatggc gacgccctgg accgaagggc ttcggacaac gcgcgtcacg tgctcgagtc
     1621 cctggccgcc accggcatcc tggcggggga cacggtgtcg gccgaccagg tatcccagat
     1681 tctcgactcc ctctacacgg accccgactc gctccgggtg gcgcgcaaca gggcggtgga
     1741 tgaggtcaac gccctgttcc tcgaggcatt cggcgccgat ctcctcgaac gcgtccggcg
     1801 ctcggacgac gcccgccgct tcgactggcg cgtgcgatgg acggtgcgga tggaggggtg
     1861 agagccgacg atgtcccgga cccttccccc ggctcgggca ttccacgggc tcggatccgg
     1921 gtatgcgcga ccgctttgcc ggatcagtcg tctccaacta cgccttcccc gacaatgacc
     1981 ctgacgtcgt cccttcgcac cggcctcgca ctcgccctgg gcctggtcct gctcgccggc
     2041 tgctcccccg actcgggctc cgcccaggtc gccgcgacgc ccgacatgaa cacgcccgtc
     2101 gtggcggcgg ccgcgtccga gcgcgcgcag cgcatcctcg agaacctgaa gtacgagatc
     2161 ccgcagctgc ggcagctccc ggtgatcatg ggtgacgttc tggacggcac cgtcgccggc
     2221 ctggaccgcg gaacgttcat ggtgcaggga cagacgtacc actacatcgt gaccgaggac
     2281 gactcgcagc tgttcatgct ggccgcgccg gcgatcgacg cgagccgctc ggccgacgac
     2341 ctcgccgccg cctacgagga ggaacaggcc gccgaggcgc gtgcggccga ggagaccgcc
     2401 tccgccctcg ccgaagcctc ctcgacgctg ccgagcctcg gacccgccga cgcgcccgtc
     2461 accatcgtcg agttctcgga cttccagtgc ccgttctgcc ggcgcgccct
//
LOCUS       DBNHQZ010000280         7853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1034482, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000280 DBNHQZ010000000
VERSION     DBNHQZ010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1034482"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1102
                     /locus_tag="AC16YU_07565"
     CDS             323..1102
                     /locus_tag="AC16YU_07565"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6065119.1"
                     /translation="MSELLVATWNINSIRARLDRLLAWLERRAPDVACLQETKCTDDQ
                     FPFDEIRAAGYEATVLGQPTYNGVAVLSRRPAGSVIRGLPGDDDDAQARFVSVDVDGL
                     RVASVYVPNGKEVGSDKYEYKLAWLDRLRAWLEREAAGRSPLVVCGDYNIAPTDDDAA
                     FPDAWAASVLCHADVRAAWDRLTAAGLRDLVREHAEPPGPWTWWDYRSLAFPRGDGLR
                     IDHLLAAPAAADRCVRTEVDRDERKGEKPSDHAPVLAWFST"
     gene            complement(1069..2175)
                     /gene="dinB"
                     /locus_tag="AC16YU_07570"
     CDS             complement(1069..2175)
                     /gene="dinB"
                     /locus_tag="AC16YU_07570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR6065120.1"
                     /translation="MRKIIHVDMDAFFASVEQRDDPSLRGRPVAVGGSTRRGVVAAAS
                     YEARAFGVHSAMPSVTAARRCPGLVFVRPRFDVYREVSGRIRDIFLSFTPLVEPLSLD
                     EAYLDVTEPISGPPSATLIARRIKALIREQTGLTASAGVSFNKFLAKVASAARKPDGL
                     TVITPADADAFLEALPVERFFGVGPATARRMHEAGIRTGADIRARAADEMARLFGKAG
                     RYYHSAAFGIDDRPVRPDRIRKSIGAERTFFEDLVSPDEMLERLRPVCEEVGRRMAGR
                     EIGALTVTLKIKRHDFRVSTRSMTLAEPARSAEVLFRAARKLLLEGSGPPEEPVRLLG
                     VTASRLVFPDDPDAMMQLSLPLGDQVENQASTGA"
     gene            2270..2707
                     /locus_tag="AC16YU_07575"
     CDS             2270..2707
                     /locus_tag="AC16YU_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065121.1"
                     /translation="MPRSRATFVFLGACLLLVPACATVVSPYSHTAYLQATSLKARSL
                     RLMDRATGPWDGSADAVERIQTDLDAALEYAKGRPRNGFSTGQWERLLDPSGHLLGGF
                     FARWAAEGTLGPTFIVEAKRLVGEAFDTISGLESGKVGAPPGP"
     gene            2704..3072
                     /locus_tag="AC16YU_07580"
     CDS             2704..3072
                     /locus_tag="AC16YU_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065122.1"
                     /translation="MIHRLMTETTENLPKRDFDTFIRQVKEALRDRLASDWPAYRSAL
                     FQIGDGFVDRTRSDMARWTGLLSTGALTRDDFEWLVFGRRELAELEALRLAGLSLAQI
                     DRFRAALVETIVGTAFRHFL"
     gene            3200..5143
                     /gene="dnaX"
                     /locus_tag="AC16YU_07585"
     CDS             3200..5143
                     /gene="dnaX"
                     /locus_tag="AC16YU_07585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR6065123.1"
                     /translation="MSEQRFLVTARKYRPQRFDELVAQEHVTATLKNAVRLERLAHAY
                     LFTGPRGVGKTTAARVLAKAINCTAPPEERSDGSEPCRTCESCRSFEEGRNLNIIEID
                     AASNNGVDDIRELRETVRVPPQGNRRKVYIVDEVHMLSKPAFNALLKTLEEPPPHVLF
                     IFATTEPHKVLPTIQSRCQRFDFRRIPVADIVEHLREICRAESIQADEGSLMLIARRG
                     DGALRDALSVFDQAVSVCGERLSQPELVEALGAVDEDLYFQVTDRITAVDTAGMLDVV
                     DTVVRSGYDLQEFLDGLAEHLRNLLVARTMPDTRLIEAAEATRTRYQDSARGLDETTI
                     LRLMSIVDQAMDQVRSSRQPRLRLELALLRMATLPSSVDLRKVLDRLGGAPAMPGPSA
                     RPASVQAAPARSRQAPRTERPAATEPTAAGTPAEPPAQRRAPESPAVSEPAGGGSEPV
                     PERGGSGPAPGPTVSGDEPPPPAGPTLFDAGPGPSVAGIRTPPRFGRPALEVSVSPSL
                     APPVEAAMADLAEPDAPGSAEPAWWPEYVERVKAERIHVGALLRHATATLAEDGALRI
                     EAPDDFHVRVLREETNLLRQALDQGDGPWARDVRIVRSSTPPGETSAADDAPDAAEVV
                     RELCEEYPALRLLMERFGGEIVW"
     gene            5191..5529
                     /locus_tag="AC16YU_07590"
     CDS             5191..5529
                     /locus_tag="AC16YU_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6065124.1"
                     /translation="MTDGMNMADMFGRMMDMQRKMAEAQEALAARTVTAEAGGGMVKV
                     TANGQQRITAITVEPAVIDPSDPEMLEDLIIAGVNKALEEAAEMARAEIQKTAGGFLP
                     PGMDLSGLGL"
     gene            5534..6160
                     /gene="recR"
                     /locus_tag="AC16YU_07595"
     CDS             5534..6160
                     /gene="recR"
                     /locus_tag="AC16YU_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6065125.1"
                     /translation="MSHSSESVENLVEQFSRLPTVGRKTARRLAAYVLKMPKEQVALL
                     AEALVAVKEKVRPCPVCFNVTDDDLCAICRAPRRDRSVICVVEEPNDVEAIERTNEYD
                     GVYHVLGGVISPLDGVGPEDLRIRDLVERIGRPPNGQDEPSVPVTEVILAMNPNVEGD
                     TTAYYIAQLLQPFGVRVSRIARGLPIGGDLEYADEATLSRALEGRGSL"
     gene            6165..>7853
                     /locus_tag="AC16YU_07600"
     CDS             6165..>7853
                     /locus_tag="AC16YU_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065126.1"
                     /translation="MRARGGASRALLLDRRGSLVLLVPVAVILAAWVLYPSLRLFAIG
                     LDPGLLGELFDDWSSANVRALWNSVRLSLLSVLGAGVLGTALATLFYRFDFPLRRLLM
                     ALAALPLALPPLVGVLAFLFLYGESGILPRGLQALLGLAEVPFAFEGFWAVWLVHVYS
                     FYVYFYLFATATLRGVDRSVLEASADLGAGGWTTFRRVLLPILRPPLVSASLLVFMLS
                     MASFTAPLLFASTEPFLTLQIYTYKINGSLDLSAAVSSVLTIICLLFLGLLELERRLG
                     SAPASKGAPAPPRPVRSGAARAAAVAVALVLLAFLVLPVLTIVLISFVEEGSWTFQIL
                     PERYTAANYVSLFSQADVLQPVVNSLTMASMATAANVVFGVATALLLVQGRIRGRAAI
                     RTIAMLPFAIPGTVIAVNLIVTFNRPDPVSFGQVLVGTFWILPLAYFIRHIPLIVRST
                     VSALEGYDERLTEASFDLGAGPVTTFRRVMLPVVGPGILAGTLLTFVAALGEFVSSIL
                     LYTFDSRPISVEILSQLRLYDFGAAAAYSVLLMAMVGASTLVVRRLGATPDRAAA"
BASE COUNT         1115 a         2886 c         2653 g         1199 t
ORIGIN      
        1 aggcagcccg tccagccccc tcgattcccg gacctgcatg ttcgaccata tggttcactc
       61 atggagagtc ggaggcgcat tgcgccctgt tctgcccgga aaggacgatc cccgcgaacg
      121 ggggacgcag gagatccttt acatcgtcgt gtaaacatgc catgcacaga agtatatcat
      181 gaacagatac acatgttcaa tatgtgcatc tggtattcca ctgcatccgg ggccccgccc
      241 cgcgatgcgg cccgcagaag gctccccgcc gaaccctccg gggcggaccg tcgtacccgg
      301 ggccggtcca cccatcgatc ccatgtccga gctgctcgtc gccacctgga acatcaactc
      361 catccgcgcc cgactggacc ggctcctcgc ctggctcgag cggcgcgccc cggacgtcgc
      421 ctgcctccag gagaccaagt gcaccgacga ccagttcccc ttcgacgaga tccgggccgc
      481 gggatacgag gccacggtgc tgggtcagcc cacctacaac ggcgtggccg tcctgagccg
      541 ccggccggcc gggtccgtga tccgcggact gccgggagac gatgacgacg cgcaggcccg
      601 cttcgtgtcg gtcgacgtcg acggcctgcg cgtggcgagc gtctacgtgc ccaacggcaa
      661 ggaggtcggc agcgacaagt acgagtacaa gctggcctgg ctcgaccggc tccgggcctg
      721 gctcgagcgc gaggcggccg gcagatcccc cctcgtcgtc tgcggcgact acaacatcgc
      781 gccgaccgac gacgacgccg cgtttcccga cgcatgggcg gcgtccgtcc tgtgccacgc
      841 ggacgtgcgg gccgcgtggg accgcctgac cgcggcggga ctgcgcgatc tggtgcgcga
      901 gcacgccgag ccgcccgggc cgtggacgtg gtgggattac cggtcgctcg ccttcccgag
      961 aggcgacggg ctgcggatcg accacctgct ggccgcaccc gccgcggcgg accggtgcgt
     1021 ccgcaccgag gtcgatcgcg acgagcgcaa gggtgagaaa ccctccgatc acgcccccgt
     1081 gctggcctgg ttctcgacct gatccccgag gggaaggctc aactgcatca tcgcgtcggg
     1141 atcgtccgga aagaccaggc gcgaggcagt gacgcccaga aggcggacgg gctcctccgg
     1201 agggcccgat ccctcgagaa gcagcttcct cgcggcgcgg aacagaacct cggccgaccg
     1261 ggcgggctcg gcgagggtca tgctgcgggt ggagacgcgg aagtcgtgcc gcttgatctt
     1321 tagcgtcacc gtgagggccc cgatctcccg ccccgccatg cgccggccca cctcctcgca
     1381 gacggggcgg agccgctcga gcatctcgtc gggtgacacg agatcctcga agaaggtcct
     1441 ctccgcgccg atcgacttgc ggatgcggtc gggccgcacg ggtcggtcgt cgatcccgaa
     1501 ggccgccgag tggtagtacc ggccggcctt gccgaagagg cgggccatct cgtccgcggc
     1561 gcgtgcacgg atgtccgcgc ccgtccggat gccggcctcg tgcatgcgcc gcgcggtcgc
     1621 gggacccacg ccgaagaacc gctcgacggg cagcgcctcc aggaacgcgt ccgcgtcggc
     1681 gggcgtgatg acggtcaggc cgtccggctt gcgggcggcg gacgcgacct tcgccaggaa
     1741 cttgttgaac gagacgcccg ccgacgccgt gaggccggtc tgctcccgga tcagggcctt
     1801 gatccgccgc gcgatgagcg tggccgacgg cgggcccgag atcggttccg tgacgtcgag
     1861 gtaggcctcg tcgagggaaa ggggttcgac gagaggcgtg aacgagagga atatgtcgcg
     1921 gatgcggccg gacacctcgc gatacacgtc gaaccgcggc ctgacgaata ccaggcccgg
     1981 gcagcggcgc gccgcggtca ccgaaggcat cgccgaatgc acgccgaacg cgcgggcctc
     2041 gtagctcgcc gcggccacca ctccgcgccg cgtcgagccg ccgacggcca ccggccggcc
     2101 gcgaagcgac gggtcgtccc gctgctcgac ggacgcgaag aacgcgtcca tgtccacgtg
     2161 gatgatcttc cgcatgcccg tccggtccag gcgtgttccg tcggcgacgc ccgccgcgcc
     2221 gatccgacgt ttctaaccgt gacccttcga tccgtttccc ccgacggcgt tgccccgatc
     2281 gcgagcgacc ttcgtcttcc tgggcgcatg cctgctcctc gtgccggcgt gcgcgacggt
     2341 cgtctcgccg tacagccaca cggcctacct gcaggccacg tcgctcaagg cgcgcagtct
     2401 ccggctgatg gaccgggcca cggggccctg ggacggctcg gcggacgcgg tcgagcggat
     2461 ccagaccgac ctggacgccg cgctcgaata cgcgaagggg cgcccgcgca acggcttctc
     2521 cacgggccag tgggagcgcc tgctggatcc gtccggacac ctgcttggcg ggttcttcgc
     2581 gcggtgggcc gcggagggca cgctcgggcc cacgttcatc gtcgaggcca agcggctcgt
     2641 gggagaggct ttcgacacca taagcggcct ggagtcgggc aaggtcggag cgccgccggg
     2701 accatgatcc atcgcctgat gaccgaaacg accgagaatc tcccgaagcg tgacttcgac
     2761 accttcatcc ggcaggtgaa ggaggcgctc cgagaccggc tggccagcga ctggccggcc
     2821 taccggtcgg cgctgttcca gatcggggac ggcttcgtgg accgcacccg cagcgacatg
     2881 gcccggtgga ccggtctcct ctcgaccggg gccctcacgc gggacgattt cgagtggctc
     2941 gtattcggcc ggcgggagct cgccgagctc gaggccctgc gcctcgccgg gctctccctc
     3001 gcccagatcg accggttccg ggccgcgctc gtcgagacca tcgtaggcac ggcgttccgg
     3061 cacttcctgt aggcccgatc ggcggctcga taggcggcgg aaccgccctc ccccggcccg
     3121 gtacgctctc ctccggaaag gcttgcagcc gcgggcccga ccggctatgt tcgcgagatc
     3181 cgtccgcccc cgccgcacca tgtccgagca gcgcttcctg gtcaccgccc gcaagtaccg
     3241 cccgcagcgc ttcgacgagc tggtggcgca ggagcacgtg acggcgacgc tgaagaacgc
     3301 ggtccgcctg gaacggctcg cccacgccta cctcttcacg ggcccgcggg gcgtcggcaa
     3361 gactacggcc gcgcgcgtcc tcgccaaggc gatcaactgc acggccccgc ccgaggagcg
     3421 ttccgacggc tcggagccct gccgcacgtg cgagtcctgc cgctccttcg aggaggggcg
     3481 caacctgaac atcatcgaga tcgacgccgc ctccaacaac ggggtggacg acatccgcga
     3541 acttcgggag acggttcggg tcccgcccca gggcaaccgc cgcaaggtgt acatcgtgga
     3601 cgaggtccac atgctcagca agccggcgtt caatgccctg ctcaagacgc tggaggagcc
     3661 gccgccccac gtcctattca tcttcgcgac gacggagccg cacaaggtgc tccccacgat
     3721 ccagtccagg tgccagaggt tcgatttccg gcgcattccg gtggccgaca tcgtggagca
     3781 cctcagggaa atctgccgtg cggagtcgat ccaggcggac gagggctccc tcatgctcat
     3841 cgcgcggcgg ggcgacggcg ccctcaggga cgcgctttcg gtattcgacc aggccgtctc
     3901 ggtctgcggc gagcggctct cccagccgga gctcgtcgag gcgctcggcg cagtggacga
     3961 ggacctgtac ttccaggtga cagaccgcat tacggccgtc gacaccgcgg gcatgctcga
     4021 cgtcgtggat acggtcgtgc gctccggcta cgacctgcag gaattcctgg acggccttgc
     4081 ggagcacctg cgcaacctcc tggtcgcgcg caccatgccc gatacccgcc tcatcgaggc
     4141 ggcggaggcc acgcggacgc ggtaccagga ctcggcccgc ggtttggacg agacgacgat
     4201 cctgcgcctg atgtcgatcg tcgaccaggc gatggaccag gtccgctcga gccgccagcc
     4261 acgcctccgg ctggaactgg cgctcctgcg gatggcgaca ctcccctcga gcgtcgacct
     4321 gcggaaagtg ctcgaccggc tgggcggcgc accggcgatg cccggcccct ccgcccgccc
     4381 cgcatccgtc caggcggccc cggcccgatc gcgccaggcc ccccgcacgg agcgccccgc
     4441 cgcgaccgag ccgaccgctg ccggaactcc cgcggagcct cccgcgcagc gccgtgcccc
     4501 ggaatcgccc gccgtgagcg agcccgccgg gggcggctcc gagccggtcc ccgagcgcgg
     4561 cggaagcggg cccgctcccg ggccgaccgt atccggggac gagccgcctc cgcccgccgg
     4621 acccacgctg ttcgatgccg gacccgggcc ctctgtggcc gggattcgca cgcccccgcg
     4681 attcgggcgg cctgccctcg aggtctcggt gtcgccctcc ctggccccgc ccgtcgaagc
     4741 cgcgatggcc gatctcgccg aacccgacgc gcccggatcg gccgagcccg cctggtggcc
     4801 cgaatacgtg gagcgggtga aggcggagcg catccacgtg ggcgcgctgc tccggcacgc
     4861 cacggcgacc ctcgccgagg acggcgccct gcgcatcgag gcccccgacg atttccacgt
     4921 ccgcgttctc cgggaggaaa cgaatctgct ccggcaggcc ctcgaccagg gggacgggcc
     4981 ttgggcccgg gacgtccgga tcgtccggag ctcgacgccg cccggcgaga cgtcggcggc
     5041 agacgatgca cccgacgccg ccgaggtcgt ccgggagctc tgtgaagaat accccgccct
     5101 ccggctcctc atggagcggt tcggcggcga gatcgtatgg tagcgggaga ccgtgccccc
     5161 gattccgacc ccgaaagaca ccgacgcacc atgacggacg gtatgaatat ggccgacatg
     5221 ttcggccgga tgatggacat gcagcggaag atggccgagg cgcaggaggc gctggccgcc
     5281 cggacggtca cggccgaggc cgggggcggc atggtcaagg tcaccgccaa cgggcagcag
     5341 cgcataacgg ccatcacggt ggagcccgcg gtgatcgatc cgtcggatcc ggaaatgctc
     5401 gaggatctga tcatcgcggg cgtcaacaag gcgctcgagg aggcggccga gatggcccgc
     5461 gcggaaatcc agaagaccgc cggcggcttc ctcccccccg gcatggacct gtccggcctc
     5521 gggctgtagc gccgtgtccc attcgtccga atccgtcgag aacctcgtcg agcagttctc
     5581 ccggctgccg accgtcggcc gcaagacggc acggcgcctg gccgcgtacg tgctcaagat
     5641 gccgaaggag caggtggcgc tcctggcgga ggcgctcgtg gcggtgaagg agaaagtccg
     5701 gccgtgtccg gtctgcttca acgtgaccga tgacgacctg tgcgcgatct gccgggcgcc
     5761 acggcgcgac cggtccgtca tctgcgtggt cgaggagccg aacgacgtcg aggccatcga
     5821 gcgcacgaac gagtacgacg gcgtctacca cgtgctcgga ggcgtcatct cgccgctcga
     5881 cggcgtcggc cctgaggacc tccggatccg cgacctcgtg gaacgcatcg gccgcccccc
     5941 gaacgggcag gacgaaccct ccgtccccgt cacggaagtt atcctggcga tgaacccgaa
     6001 tgtcgagggc gacaccacgg cctactacat cgcccagctc ctgcagccgt tcggcgtacg
     6061 ggtatcgcgc atcgcgcgcg gcctgccgat cggcggagac ctggagtacg cggacgaggc
     6121 cacgctgagc cgcgccctcg agggccgcgg aagcctgtag ccgcatgcgc gcgcgcggcg
     6181 gagcctcccg ggccctcctg ctggaccggc gcggcagcct ggtgctcctg gtccctgtcg
     6241 ccgtgatcct cgcagcctgg gtgctgtacc ccagcctgcg gctcttcgcg ataggcctcg
     6301 accccggcct cctcggcgag ctcttcgacg actggtcgtc ggcgaacgtc cgcgccctgt
     6361 ggaattccgt gcggctctcg ctgctgagcg tcctcggtgc cggcgtgctg ggtacggcgc
     6421 tcgccaccct cttctaccgg ttcgacttcc cgctcaggcg cctgctcatg gcgctcgccg
     6481 ccctgccgct cgctctgccc ccgctcgtcg gcgtgctcgc gttcctcttc ctgtacggcg
     6541 agagcggcat cctgccgcgc ggccttcagg ccctgctcgg tctcgcggag gtgccgttcg
     6601 ccttcgaggg cttctgggcg gtgtggctcg tccacgtcta ttcgttctac gtctacttct
     6661 atctcttcgc gacggcgacg ctccggggcg tcgaccggtc cgtgctcgag gcgtcggccg
     6721 acctgggggc cggcggctgg acgacgtttc gccgcgtgct cctccccatc ctgcggccgc
     6781 cgctcgtcag cgcctcgctg ctcgtgttca tgctcagcat ggcgtccttc acggctccgc
     6841 tgctcttcgc ctccacggag cccttcctca cgctccagat ctacacctac aagatcaacg
     6901 gaagcctgga cctctcggcg gccgtgtcgt cggtcctgac catcatctgc ctgctctttc
     6961 tcgggctgct cgagctcgag cggcgtctcg gatcggctcc cgcgtcgaag ggcgccccgg
     7021 ccccgccgcg cccggtgcgc tcgggggcgg cccgcgcggc cgccgtcgcc gtggcgctcg
     7081 tgctcctggc cttcctggtc ctgccggtgc tcacgatcgt cctgatctcg ttcgtcgagg
     7141 agggatcctg gaccttccag atccttccgg agcgctacac ggccgcgaac tacgtctcgc
     7201 tcttctcgca ggccgatgtg ctgcagccgg tcgtgaacag cctcacgatg gcctccatgg
     7261 ccacggccgc gaacgtcgtg ttcggcgtgg cgacggcgct gcttctcgtg cagggccgca
     7321 tccgcgggcg ggcggccatc cggaccatcg cgatgctccc gttcgcgatc cccggaacgg
     7381 tgatcgccgt gaacctgatc gtgacgttca accgtccgga tcccgtgtcc ttcggccagg
     7441 tgctggtcgg cacgttctgg atcctgccgc tggcgtactt catccggcac atcccgctca
     7501 tcgtacgctc gaccgtctcc gcgctcgagg ggtacgacga acggctgacc gaggcgtcgt
     7561 tcgacctcgg ggcgggaccg gtcacgacgt tccgccgcgt gatgctgccc gtcgtcggcc
     7621 cgggcatcct cgcgggcacg ctcctcacgt tcgtggcggc gctcggcgaa ttcgtgtcgt
     7681 cgatcctgct ctacacgttc gactcgaggc cgatctcggt cgagatcctg tcccagctcc
     7741 ggctgtacga cttcggcgcg gccgccgcct acagcgttct gctcatggcg atggtgggcg
     7801 catccaccct cgtggtccgc cggctcggcg ccacgcccga ccgggcggcc gcc
//
LOCUS       DBNHQZ010000281         5467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3062280, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000281 DBNHQZ010000000
VERSION     DBNHQZ010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3062280"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3380)
                     /locus_tag="AC16YU_07605"
     CDS             complement(<1..3380)
                     /locus_tag="AC16YU_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065127.1"
                     /translation="MQTCRNRSLRPNPFALAALTVWLAALPALAQTRETGWSYPEVRA
                     WINRTTARAGSPALADCFVDRTSRRDAVLNGNRITTQILNFGSISSPGNTITDIVWNG
                     LGYGYEFGPFVAAEVVDEGHRDPKSVMKRDENGEVVLDDNGDPVWVMHIVSDGLVSNG
                     GEISPDGSEFWGWQPIPCAQPVGAFEGIEVVAEDSDLIPTSDAPDRNLDGRPDSWPPS
                     WYNPTLREYVWPGALQQGASNSDKEALYFMNDYNNREFAYWPIPSDSSLRGLGLEVEV
                     RLYQWANPLAEDAIFLVYKITNKSEKDLDKVAFGMWGDPHVGGPSDWADDLAFFDRDL
                     NMVFAWDNDGRSDIAGRAPGYFGYKFLESPGLGDEIIDGVFFPGDGIDNDGDGMVDES
                     WTDGIDNDGDWDPETDDVGIDGVPGTGDEGEGDGVPTAGDHYDITRPGEPNFEFTDID
                     ESDMIGLTSFASPRFAGSQVRNDEQVWGWIEPGRFDDVPAEPGDYVFLYGSGDFPLRA
                     GETKRFSIALIVGENLNDLRLNAETVQQIFNVGYRFARPPDKPKVRAVPGDRKVTLYW
                     DSAAEQSVDPLSREQDFEGYVIYRSTDHEFSDQQTITDINGSRFLFRPLSTEVGVDAR
                     FDLDNGISGPSPIPYPQRGVAYHLGNDTGLFHTFVDSNNVVNGQTYYYSVVAYDRGWS
                     GAGDADFSDGIAPSESAKTITYNPVFDDYIFDVNTVSVVPGPVSAGYSGPVVGVEGGL
                     VRVGGAGTGDITIRTVDPMAIPEGGQYRIDFDPAGSGVSYSVTDLVTRTVRILAAEGR
                     SASLGVPNVIPESVQIVDDAGGVLEQGTHYTVNEENGSVVLEPGAPVTGDSLTATFRH
                     RPLVGSTLLDNEEANLVFDGMHFFVRNEPLRINSAETGWAMGGQGIPFEVRVATSGPG
                     RVAQPADYEITFFGENVTTGFNNNLPLPFRIVNVTRANEEIQAFVPDINQNGSWDVGE
                     QIIFLETIGDVLTATWQVAFTDVGSRPGAGDVFYVRTDKPFTDEDAFTLTTAGAFADP
                     ARLAADIDDCDDPLGECIYVVPNPYVATNEIEPRNPSSRSERGDRRLYFAGVPAKCTI
                     RIFTLAGELVDTIEHDSPVDDGKVFWDLRTKDNMNIAYG"
     gene            complement(3419..>5467)
                     /locus_tag="AC16YU_07610"
     CDS             complement(3419..>5467)
                     /locus_tag="AC16YU_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6065128.1"
                     /translation="YGGDTVTRNDDVFMTPDSRRLNVLTLEGSLSGPVPFFRKVRFFV
                     SGRRDIDDGHIWGRREHLPSDSANFNSDPARYALIQQFIPGYDGPDWYYELHGKPWYE
                     YGPDEEIPGELVNLNPSRSANVIAKLTTRPIRGSKLEYSVLHDQATRTPFSFADRFNP
                     DGVWSTRDRSWNHSVHWTQSLGERTFYNLRMSYAKNRARSYLYEDPADPRYVSTGAIV
                     GFPGNHFVFGGNDKSYIVEEARSLRVKADVTRQLGLIHEAKVGVDFQRHGLDRQNFAI
                     LYDGDQYTEPTVLPPDESPSHDLYEDQRVTEFSLYAQDKLEFEDFIINVGLRYERFDP
                     HGKYVQNLLDPLLRQSTLADAEVKNLLLPRLGVSFPITETGIIHFSYGHFAQMPSLRT
                     MYVNPEFEFPVGVGPQFGNTNMRPERTVQYEIGLQQQLGDELAFNVTGFFKDIRDYLA
                     SQTVRFSTVSGEDVYSVYLNKDYANVKGVTVSLTRRRPRGGLLSAGIDYTFQMAEGNN
                     NDANAFFFNYLSGRETELELVPLSFDQRHMISGTVGLTRPGNWGLSVIGQFGTGYPYT
                     PLLIDQKIDQLPNQERKPAQIKLDAHLYKDFRLPGETRLRLFGKVFNVLDRLNERFVF
                     NDTGRATYSLSAERGTFAAWEAAVARSLPGVHPLEEYDTRPHWYSAPREIRLGATLSF
                     "
BASE COUNT          860 a         1751 c         1877 g          979 t
ORIGIN      
        1 ccgtaggcga tgttcatgtt gtccttggtc ctgagatccc agaacacctt gccgtcgtcg
       61 accgggctgt cgtgctcgat cgtgtcgacc agctcgccgg cgagtgtgaa gatccggatc
      121 gtgcactttg ccggaacccc cgcgaagtac agccggcggt cgccccgctc cgagcgcgag
      181 ctcgggttgc gcggttcgat ctcgttggtc gcgacgtacg ggttgggcac gacgtagatg
      241 cactcgccca ggggatcgtc gcagtcgtcg atgtcggcgg cgagtcgcgc cggatcggcg
      301 aaggcaccgg cggtcgtgag cgtgaacgcg tcctcgtcgg tgaagggctt gtcggtgcgc
      361 acgtagaaga cgtcgccggc ccccggccgg ctgcccacgt cggtgaaggc gacctgccag
      421 gtcgcggtca gcacatcgcc aatcgtctcg aggaagatga tctgttcacc cacgtcccat
      481 gagccgttct ggttgatgtc cggcacgaag gcctggatct cctcgttcgc ccgcgtgacg
      541 ttcacgatcc ggaaggggag cggcaggttg ttgttgaagc ccgtagtcac gttctcgcca
      601 aagaacgtga tctcgtagtc cgcgggctgc gccacgcgcc caggtccgga cgtcgccacg
      661 cggacctcga agggtatgcc ctgtccgccc atcgcccacc ccgtctccgc gctgttgatg
      721 cgaagcggct cgttccggac gaagaagtgc atgccatcga agacgagatt cgcctcctcg
      781 ttgtccagga gcgtggagcc gaccagcggg cggtggcgga aagtggccgt cagcgagtcc
      841 cccgtgaccg gggcgccggg ctcgaggacg accgagccgt tctcctcgtt gaccgtgtag
      901 tgggtgccct gctccagcac accgcccgcg tcgtccacga tctgcacgga ttccgggatt
      961 acgttcggga ctccgagcga cgcggagcgg ccctccgccg cgaggatccg cacggtgcga
     1021 gtcacgaggt ccgtcaccga gtaggacact ccgctgccgg ccgggtcgaa gtcgatgcgg
     1081 tactgcccgc cctccgggat cgccatcggg tccacggtgc ggatcgtgat gtcgcccgtg
     1141 cctgcccctc ccacccggac caggccgcct tcgacgccga cgacgggacc ggagtacccg
     1201 gccgagaccg gtccgggcac gacggacacc gtgttcacgt cgaagatgta gtcgtcgaag
     1261 acggggttgt acgtgatggt cttggcggac tcgctcggcg cgatgccgtc ggagaagtcg
     1321 gcatccccgg cgcccgacca gccgcggtcg taggccacga ccgagtagta gtaggtctgg
     1381 ccgttcacca cgttgttcga gtccacgaac gtgtggaaga ggcccgtgtc gtttccgagg
     1441 tggtacgcca cgccccgctg cggatagggg atcggggagg ggccgctgat cccgttgtcg
     1501 aggtcgaacc gagcgtctac ccccacctcg gtcgaaagcg gccggaacag gaatcgcgac
     1561 ccgttgatgt cggtgatggt ctgctggtcc gagaactcgt ggtccgtgga ccggtagatg
     1621 acgtacccct cgaagtcctg ctcccgcgag agcgggtcga cggactgctc ggccgcgctg
     1681 tcccagtaga gcgtcacctt gcggtcgccg ggcacggcgc ggactttggg cttgtcggga
     1741 ggccgggcga agcggtaccc cacgttgaag atctgctgga cggtctcggc gttaaggcgc
     1801 aggtcgttca ggttctcgcc cacgatgagg gcgatcgaga agcgcttggt ctcgcccgca
     1861 cgcaggggga agtctcccga gccgtacagg aacacgtaat cgccgggctc ggccggcacg
     1921 tcgtcgaaac gacctggctc gatccacccc cagacctgct cgtcgttgcg cacctggctg
     1981 cccgcgaaac ggggcgacgc gaaggacgtg agtccgatca tgtcggactc gtcgatgtcc
     2041 gtgaactcga agttcggctc gccgggccgg gtgatgtcgt agtggtcgcc cgccgtgggc
     2101 accccgtcgc cctcgccctc gtcgcccgtc ccggggacgc cgtcgatgcc gacgtcgtcc
     2161 gtctccgggt cccagtcgcc gtcgttgtct atcccgtcgg tccacgattc atcgaccatg
     2221 ccgtcaccgt cgttgtcgat cccgtcaccc ggaaagaaga ccccgtcgat gatctcgtcg
     2281 ccgagcccgg ggctctcgag aaacttgtag ccgaagtacc ccggcgcacg gcccgcgatg
     2341 tcggaccggc cgtcgttgtc ccaggcgaag accatgttca ggtccctgtc gaaaaacgcc
     2401 aggtcgtccg cccagtcgct cgggccgccg acgtgggggt caccccacat gccgaacgcc
     2461 accttgtcca ggtctttctc gctcttgttc gtgatcttgt agacgaggaa gatggcatcc
     2521 tcggcaaggg gattggccca ctggtacagg cgcacctcga cctcgagccc caggccgcgg
     2581 agggacgagt cgctcgggat cggccagtac gcgaattccc ggttgttgta gtcgttcatg
     2641 aagtagagcg cttccttgtc cgagttggag gcgccctgct gcagggctcc cggccacacg
     2701 tattcccgca gggtcgggtt gtaccacgag gggggccacg agtcgggccg accgtccagg
     2761 ttccggtcgg gcgcgtcgga cgtcggaatc aggtcggaat cctcggccac gacctcgatg
     2821 ccctcgaacg cgcccacggg ctgggcgcac gggatcggct gccagcccca gaactcgctc
     2881 ccgtccgggg agatctcgcc gccgttcgag acgagcccgt cggaaacgat gtgcatgacc
     2941 cagaccgggt cgccgttgtc gtcgagcacc acctcgccgt tctcgtcgcg cttcatcacg
     3001 ctcttggggt cacggtgccc ctcgtccacg acctcggcgg ccacgaacgg cccgaactcg
     3061 tagccgtaac ccaggccgtt ccagacgatg tccgtgatcg tgttgccggg cgacgagatc
     3121 gagccgaagt tcaggatctg cgtcgtgatc cggttgccgt tgagcacggc gtcccggcgc
     3181 gaggtcctgt ccacgaagca gtctgcgagg gcaggagatc ccgctctcgc cgtggtccgg
     3241 ttgatccagg ccctgacctc gggataggac catcccgtct cgcgggtctg ggccagcgcg
     3301 ggaagggcgg cgagccatac ggtcagcgcc gcgagcgcga acgggttcgg gcggagggac
     3361 cggttacggc acgtctgcat gggtgtcatc aggcggtcga ttgagcgctt gggatcggtc
     3421 agaaggagag cgtggcgccg agacggatct cgcgcggggc ggaataccag tgcgggcgag
     3481 tgtcgtactc ttcgagcggg tggacgcccg gcagcgaccg cgcgacggcc gcctcccatg
     3541 cggcgaacgt gccgcgctcg gccgagagcg agtacgtcgc cctccccgtg tcgttgaaca
     3601 cgaagcgctc gttcaggcgg tcgagcacgt tgaacacctt gccgaacagg cgaagtctcg
     3661 tttctccggg aaggcggaaa tccttgtaga ggtgggcatc gagcttgatc tgcgcgggct
     3721 tccgctcctg gttgggcagc tggtcgatct tctggtcgat gagcagcggc gtgtacggat
     3781 agccggtgcc gaactggccg atgaccgaca gtccccagtt gcccggacgg gtcaggccca
     3841 ccgtgcccga gatcatgtgc cgctggtcga agctgagcgg aacgagctcc agctccgtct
     3901 cgcgcccgga caggtagttg aagaagaagg cgttcgcgtc gttgttgttg ccttcggcca
     3961 tctggaacgt gtagtcgatg ccggcagaca gcagcccgcc ccgcggacgg cggcgcgtaa
     4021 gtgacacggt gacgcccttg acgttggcgt agtccttgtt caggtacacg ctgtagacgt
     4081 cctcgccgga cacggtcgag aagcggaccg tctgcgaggc gaggtagtcc cggatgtcct
     4141 tgaagaaccc ggtgacgttg aaggccagct cgtctccgag ctgctgctgc agcccgatct
     4201 cgtactgcac cgtgcgctcg ggacgcatgt tcgtgttgcc gaactgcggt ccgacgccca
     4261 cagggaattc gaactcgggg ttcacgtaca tcgtgcgcag cgacggcatc tgggcgaaat
     4321 gcccgtacga gaagtggatg atgccggtct cggtgatcgg gaagctcacg cccaggcgcg
     4381 gcaagaggag gttcttgacc tccgcgtcgg cgagggtgga ctggcgcagg agcgggtcca
     4441 gcaggttctg gacgtacttg ccgtgcgggt cgaagcgttc gtaccggagg cccacgttga
     4501 tgatgaaatc ctcgaattcc agcttgtcct gggcatagag gctgaactcg gtcacacgct
     4561 ggtcctcgta caggtcgtgc gagggcgact cgtccggcgg caggacggtc ggctcggtgt
     4621 actgatcgcc gtcgtaaagg atggcgaagt tctggcggtc caggccgtgc cgctggaaat
     4681 cgacgcccac cttcgcctcg tggatgagtc cgagctggcg cgtgacgtcc gccttgacgc
     4741 ggagcgagcg ggcctcctcg acgatgtacg acttgtcgtt gccgccgaag acgaagtggt
     4801 tgcctgggaa gccgacgatc gcgcccgtgg agacgtagcg cgggtcggcc ggatcctcgt
     4861 acaggtacga cctggcccgg tttttcgcgt acgacatgcg aaggttgtag aacgtgcgct
     4921 cgccgagcga ctgggtccag tgcacgctgt ggttccacga gcggtcgcgc gtgctccaca
     4981 ccccgtcggg attgaaccgg tccgcgaagc tgaacggcgt ccgcgtcgcc tggtcgtgca
     5041 ggaccgagta ctccagcttg ctgccgcgga tcggacgcgt cgtgagcttc gcgatcacgt
     5101 tcgcgctgcg cgacgggttc aggttcacga gctcaccggg gatctcttcg tccggtccgt
     5161 actcgtacca gggctttccg tgcagctcgt agtaccagtc gggcccgtcg tacccgggaa
     5221 tgaactgctg gatgagcgcg taccgcgccg ggtccgagtt gaagttcgcc gagtccgacg
     5281 gcaggtgttc gcgccggccc cagatgtgcc cgtcgtcgat gtcccgccgc ccggagacga
     5341 agaaccggac ctttcggaag aagggcaccg gaccgctcag cgatccctcg agggtgagca
     5401 cgttcaggcg ccgcgagtcc ggggtcatga agacgtcgtc gttccgggtg accgtgtcgc
     5461 ccccgta
//
LOCUS       DBNHQZ010000282         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2388087, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000282 DBNHQZ010000000
VERSION     DBNHQZ010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2388087"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC16YU_07615"
     CDS             complement(<1..752)
                     /locus_tag="AC16YU_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6065129.1"
                     /translation="MHATDDQRPAWIYPLLAAGVLSFASSAILIRYAGSAPGLAVAVF
                     RTAIAVLILAPAGLRATWREWRPVVWKDVGLVLLAGALLGLHFVAWISSVYLTTIASS
                     AVLVTTGPIFLAALGFLVLGERLEPRVILSILIAVAGAAVLAIGESAGEAAPGRNPAL
                     GNALALFAAMVWAVYLLIGRVVRQRLGWFAYVFPLYVVAAILAVVAAVVADVRLTGYD
                     LSIYGLCALMAIFPQILGHGSFNFAVKYFPAAL"
     gene            complement(801..1217)
                     /locus_tag="AC16YU_07620"
     CDS             complement(801..1217)
                     /locus_tag="AC16YU_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HIR6065130.1"
                     /translation="MIQRIQTVFLAVGAVLVAVAPFAGELWRVPATEVPWFVPAVGVA
                     LAAAFAASAIAIFLFRMRERQRTFVVVAQLLTLAALVALFFGRRATATLPGAEGATTE
                     DWLLFVMPAVAYVLFLLARRAIDRDIRLLKSVDRLR"
     gene            complement(1252..>1672)
                     /locus_tag="AC16YU_07625"
     CDS             complement(1252..>1672)
                     /locus_tag="AC16YU_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6065131.1"
                     /translation="DRIIDNMCMQLVVRPQEYRCLVTTNLFGDILSDLAAGLVGGLGV
                     VAGANIGDRYAVFEAVHGSAPDIAGRGIANPTALVRSAEMMLRHLGEIRAADALHEGL
                     VDVYTAGDTLTADQGGSASTDEFARHLAERVAVRARA"
BASE COUNT          294 a          569 c          618 g          191 t
ORIGIN      
        1 agcgccgccg ggaaatactt gaccgcgaag ttgaacgatc catggcccag gatctgcgga
       61 aagatggcca tgagcgcgca caggccgtag atcgagaggt cgtagcccgt gagccggacg
      121 tcggccacga ccgcggccac gacggccagg atggccgcga cgacgtacag gggaaagacg
      181 tatgcgaacc agccgagccg ctgcctgacc acgcgtccga tgagcaggta caccgcccag
      241 accatcgcgg cgaagagcgc aagcgcgttg cccagggcgg ggttccgccc cggcgccgcc
      301 tctcccgcgg attcgccgat cgcgaggacc gcggcgccgg ccacggcgat aagaatcgaa
      361 aggatcacgc gcggctccag ccgctcgccg agtaccagga agccgagggc ggcgaggaag
      421 atcgggccgg tcgtgaccag gacggccgag ctcgcgatgg tcgtcaggta gacggacgag
      481 atccacgcca cgaaatgcag gccgaggagt gccccggcga ggagcaccag gccgacgtcc
      541 ttccacacga cgggccgcca ctcgcgccag gtcgcgcgaa ggcccgcggg agcgaggatc
      601 aggaccgcga tggcggtgcg gaagacggcc accgcgaggc cgggggccga cccggcgtac
      661 cggatcagga tggcgctcga ggcgaagctc aggaccccgg ccgcgagcaa cgggtagatc
      721 caggcggggc gctggtcgtc ggtggcgtgc atgggaggtg cgtcgtcgga cctgcgggca
      781 cggtcgatgg cccggcgggc tcagcgcagg cggtccaccg acttcaggag ccggatgtca
      841 cggtcgatgg cccggcgggc caggaggaac agcacgtagg ccacggccgg catcacgaac
      901 aggagccagt cctccgtcgt cgcgccctcg gcgcccggca gcgtggccgt ggcacggcgg
      961 ccgaagaaga gggcgacgag cgccgccagc gtcaggagct gggcgacgac cacgaacgtc
     1021 cgctgcctct cgcgcatgcg gaacaggaag atggcgatcg ccgaggccgc gaaggctgcg
     1081 gcgagcgcga cgccgaccgc gggcacgaac cacggaacct ccgtcgccgg aaccctccac
     1141 agttccccgg cgaacggcgc cacggcgacg agtacggcgc cgacggccag gaagacggtc
     1201 tggatgcgct ggatcatggt cttggaggat tggggtcgag tcgcgccggg atcaggccct
     1261 ggctcggacg gccacgcgct cggccaggtg ccgggcgaac tcgtcggtcg aggccgagcc
     1321 gccctggtcg gccgtgagcg tgtcgccggc cgtgtacacg tcgaccagcc cttcgtgcag
     1381 cgcgtcggcg gcccggattt cgcccaggtg ccgcagcatc atctcggccg accggacgag
     1441 tgcggtggga ttggcgatgc ctcggccggc gatgtcaggg gccgatccgt gcacggcctc
     1501 gaatacggca taccggtccc cgatgttcgc ccccgccacc acgcccagcc cgccgacgag
     1561 ccccgccgcc aggtcactga ggatgtcccc gaacaggttc gtcgtcacca ggcatcggta
     1621 ctcctgcggc cgcaccacga gctgcatgca catgttgtcg atgatgcggt cg
//
LOCUS       DBNHQZ010000283         5602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3337584, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000283 DBNHQZ010000000
VERSION     DBNHQZ010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3337584"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="AC16YU_07630"
     CDS             <1..1882
                     /locus_tag="AC16YU_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6065132.1"
                     /translation="SASIAAAGASGGAFAGQAPVSASSMSAAAAGPPVRRGRVVGPAV
                     LGAVAAATLLVGLAAGWVFRPTPDAPAPTPRKLSVALDGYREVVFPTASADGRYWAFR
                     SIDTTGTSGIVILDARTGSTSIVPESSGYFYSLFSPSGKWILMTGSGRLARALVSGGR
                     PIDMGGVSDVGLDWLDEERIVTASVQGSAHEFHVVSIADNSSVSVPIRGLDSTYVEMA
                     YVRRVPGTDILISSWNRRDLSASLVAIDLTSGAATVLKQGACCASPVRGDHLVYLTGA
                     GEGQLVAQAFDRDGLRLTGAPVDLLDSNIDEVGFGVGLDGSLMIGGSLVEQGGERLTW
                     LDPETRQTEEVGLPVNNYDRVSLSPDGRRAAIERRTDGDNRGDIYVMNLLDGTEQRLT
                     YNHAINRVPVWSRSGRIYFAGGAQGQSDIYVKNADGSGPDELLVRNGDFPHVSEDEEW
                     LAFVRSRAHNHIFALNLRTGEEVVIDTTGLKTSDPTISPDGRYIAFSADPQKGPRVSQ
                     EQRVYVRSFPDGSQLYQLVSDDRGDDPMWSPDGRWLYYRSRGQFFRMAVSTEGTFQKL
                     SLPDPIGSFAGVTVRGSVDRSKGRLLIMHPGDAGSVVNSTRMTLYDAFPTLLHEVAPA
                     SR"
     gene            1896..2705
                     /locus_tag="AC16YU_07635"
     CDS             1896..2705
                     /locus_tag="AC16YU_07635"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6065133.1"
                     /translation="MPDLRILDETYGLTPEQVAHYREHAWIRLKDVLPADVVTHFDRT
                     ITADVLRLNRETRPLEERDTYGRAFLQVMNLWRESDAARTLVFSRRLAGIAAGLMETD
                     GVRLYHDQALYKEAGGGHTPWHADQYYWPLATDRTVTLWLPLQPTPLGMGPLEFSEGS
                     HRMAAGRDLAISDESEEAIERALANGQYRHVVEPFEIGDASFHAGWLFHRAGPNTSGT
                     PRRVMTVIYMDAAMRLKEPDNENQELDRDTWCPGARVGEVIDTPLNPVLYP"
     gene            2826..3866
                     /locus_tag="AC16YU_07640"
     CDS             2826..3866
                     /locus_tag="AC16YU_07640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065134.1"
                     /translation="MPRHLLAAVLATLVVLPCGAQGLGRGTPGAPVLDWPTADPDSLG
                     LDLEALERHRVLCAESEASGCLVAYRGVIVQEWYSPDFPADPLPMQPWIGTRSATKSV
                     IGLLAGMLVGDGAIGSVDDSTARYLPEWAAGAEAGVTLRHLLTMTSGVARHSGEGPHP
                     GVVAAKNTRGFVLGLPLQGVPGERWAYSNEGAQLLSPILERAAGMPLSAYARERLFDP
                     IGMETTHLLVDEYYSTVAIGGMQTRLREFARIGQLVANGGAWNGEQIVPAAWIEASTT
                     PIEQNPYYGFLWWIDRELGAVSAAGTFDQVILVFPELDLVAARLQRDVESGRNGHYWN
                     RETMALLRDVVE"
     gene            complement(3886..>5602)
                     /locus_tag="AC16YU_07645"
     CDS             complement(3886..>5602)
                     /locus_tag="AC16YU_07645"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR6065135.1"
                     /translation="EQMTLDEKIGQMTQPDQEFLRSVDDIRDYFLGSVLSGGGSDPDT
                     NTFDAWRAMAERYQAKALETRLRIPLLYGVDAVHGHNNVIGAVVFPHNVGLGATRNAA
                     LVEEVGRITAKEVRATGINWTFAPCVAVPRDDRWGRTYEGFSESPAIVAELGAAAVRG
                     FQGTDLSDPLRIVACAKHFAGDGGTMPGTGLPRGEELLLDQGDTRLTEEELRRIHMEG
                     YVTAIEAGVATIMPSYNSWNGEKVSGSRRLLTEILKQELGFEGFLISDYRAIDQLPGD
                     YRSDIEQSINAGMDMVMVPDRYQEFIETLRSLVEEGAVPMERIDDAVRRILRVKFAAG
                     LFDEDWSPTADRSLEASFGSDAHREVARRAVRESLVLLRNEGGVLPLVKGVERIHVAG
                     SGADDIGMQSGGWTIEWQGALGPITDGTTIIEGIRAAVAEGTEVTFSGDASGSEGADV
                     VVVVVGERPYAEMLGDDLALRLSEEDHRAVAAAKASGAPVVTVVLSGRPVILGDILEA
                     SDAVVAAWLPGTEGQGVADVLFGDYAPTGKLAFTWPRSADQHPINEGDAAYDPLFPFG
                     FGLTY"
BASE COUNT          835 a         1948 c         1925 g          894 t
ORIGIN      
        1 gtcagcgtcc atcgcggccg caggggcgag cggcggggcg ttcgccggcc aggcgccggt
       61 gtcggcgtct tcgatgtccg ccgccgccgc ggggccgccc gtgcgccgtg gccgggtggt
      121 cggcccagcg gtgttgggcg ccgtggccgc tgccacgctg ctcgtcggac tcgccgcggg
      181 gtgggtgttc cgtcccactc cagacgcccc cgcgccgacc ccgcgcaagc tctcggtggc
      241 cctcgacggc taccgcgaag tggtctttcc gaccgcctcg gccgacgggc ggtactgggc
      301 gttccgctcg atcgacacga cagggacctc gggcatcgtg atcttggacg cccgtacggg
      361 atcgacctcc atcgttcccg agtcgtccgg ctatttctac tccctcttct ccccgtccgg
      421 aaagtggatc ctcatgacgg gaagcggccg cctcgcgagg gctctcgttt cgggcggaag
      481 gcccatcgat atggggggag tttccgacgt cggcctggac tggctggacg aggagcgtat
      541 cgtcaccgcg tccgtccagg ggagcgccca cgagtttcat gtcgtctcga tagcggacaa
      601 ttcgagcgtc tccgtgccca tccgcgggct cgactcgacg tatgtcgaga tggcttacgt
      661 gcgtcgcgtg cccggaaccg atatcctgat cagttcctgg aaccggcgcg acctctcggc
      721 ctcgctcgtc gccatcgacc tgacgtcggg cgcggccacc gtgctgaagc agggggcctg
      781 ctgtgcatcg cccgtccggg gcgaccacct ggtgtacctg accggggcgg gcgaggggca
      841 gcttgtggcc caggcattcg accgtgacgg cctcaggctc accggtgcgc cggtggatct
      901 gctcgattcg aatatcgacg aggtgggctt cggcgttggg ctcgacggat cgctcatgat
      961 cggcggctcc ctggtcgagc agggcgggga acggctcacg tggcttgacc ccgagacgag
     1021 gcagaccgaa gaggtcgggc tgcccgtcaa caactacgac cgggtgagtc tctcgcccga
     1081 cggccgacgg gcggccatcg agcggcgcac ggacggcgac aaccggggcg acatctacgt
     1141 catgaacctg ctcgacggga ccgagcagcg cctgacctac aaccacgcca tcaaccgcgt
     1201 cccggtctgg agccgaagcg gccgcatcta tttcgcgggc ggcgcgcagg ggcagtcgga
     1261 catctacgtc aagaacgctg acgggtccgg tcccgacgag ctcctggtcc ggaacggcga
     1321 cttcccgcac gtttcggagg acgaggagtg gctggcattc gtccggtcac gcgcccacaa
     1381 ccacatcttc gcgctgaatc tgcggaccgg tgaggaggtc gtgatcgaca cgacgggact
     1441 caagacctcc gatcccacga tcagccccga cggtcgctac atcgcgttca gcgccgatcc
     1501 ccagaagggg ccgcgcgtgt cccaggagca gcgggtatac gtgcgctcgt tccccgacgg
     1561 gtcgcagctg taccagctgg tgtcggacga ccggggtgac gacccgatgt ggtcaccgga
     1621 cggcaggtgg ctctactacc ggagccgtgg ccagttcttc cgcatggccg tcagcaccga
     1681 gggcacgttc cagaagctct cgctccccga tccgatcggc tcgttcgcgg gagtgacggt
     1741 gcgcggctcg gtcgatcgct cgaagggccg gctgctcatc atgcacccgg gcgacgcagg
     1801 atccgtcgtc aattcgaccc ggatgaccct gtacgacgct ttcccgacgc tcctgcacga
     1861 ggtggcgccc gcctcgaggt agacaccgaa cgaccgtgcc cgaccttcgc atcctcgacg
     1921 agacctacgg gctcaccccc gaacaggtcg cccattaccg cgaacacgcc tggatcaggc
     1981 tgaaggacgt gctgccggcc gacgtggtca cgcacttcga ccggacgatc acggccgatg
     2041 tcctgcgcct gaatcgggag acccgtcccc tggaggagcg ggacacctac ggccgcgcct
     2101 tcctccaggt catgaacctg tggcgggaaa gcgacgccgc gcggacgctc gtcttcagcc
     2161 gccgcctcgc gggcatcgcg gcaggtctga tggaaacgga cggcgtgcgg ctctaccacg
     2221 accaggcgct gtacaaggag gcggggggag ggcacacgcc ctggcacgcc gaccagtatt
     2281 actggccgct ggcgaccgac cgtacggtca cgctctggct cccgctgcag ccgaccccgc
     2341 tcgggatggg tcccctcgag ttcagcgagg gcagccaccg gatggcggcc ggccgcgacc
     2401 tcgcgatcag cgacgagagc gaggaagcga tcgagcgggc gctggcaaac gggcaatacc
     2461 gccacgtggt cgagccgttc gagatcggcg acgccagctt ccacgccggc tggctgttcc
     2521 atcgcgcggg cccgaacacg tccggcacgc cccgccgcgt catgaccgtg atctacatgg
     2581 acgcggccat gcggctcaag gagccggata acgaaaacca ggagctggac cgggacacat
     2641 ggtgcccggg cgcccgggtg ggggaagtga tcgacacgcc gctgaaccct gtgctgtatc
     2701 cctgaccggc gtccgggtga cgggcacggt gacgatggtc cggaggccgg tgaatgacac
     2761 ggcggtgatt gacctccggt gtcgctacga ctatattagt actaccttgt tagtttaata
     2821 tcgaaatgcc tcgtcatctc ctcgcggcgg tgctcgcgac gctcgtcgtc cttccctgcg
     2881 gtgcccaggg ccttggccgg ggcacgcccg gtgccccggt cctcgattgg cctaccgccg
     2941 accccgattc tctcggactg gatctggagg ccctggagcg tcaccgggtg ctttgcgcgg
     3001 aatcggaggc ctcgggatgt ctggtggcct accggggggt gatcgtgcag gagtggtatt
     3061 cgcccgactt tccggccgat ccgctgccca tgcagccctg gatcggcacg cggtcggcga
     3121 cgaaatcggt catcggcctg ctcgccggca tgctcgtcgg agacggcgcc atcggaagcg
     3181 tggacgattc caccgcgagg tacctgcccg agtgggcggc cggagccgag gccggcgtga
     3241 cgctcaggca cctcctcacg atgacgtccg gcgtggcgcg ccactccggg gaaggtccgc
     3301 atccgggggt cgtggccgcg aagaacacgc gcgggttcgt gctgggcctg cccctgcagg
     3361 gcgtgccggg cgagcgctgg gcctacagca acgagggcgc gcagctcctc tcgccgatcc
     3421 tggagcgcgc ggcgggcatg ccgctgtcgg cgtatgcccg cgagcgcctg ttcgacccga
     3481 tcgggatgga aacgacgcac ctcctcgtgg acgagtacta ctcgaccgtc gcgatcgggg
     3541 gcatgcagac ccgcctgcgc gagttcgcgc gcatcgggca gctcgtggcc aacggcgggg
     3601 cgtggaacgg cgagcagatc gtgcccgccg cgtggatcga ggcgtccacg acacccatcg
     3661 agcagaaccc gtactacgga ttcctgtggt ggattgaccg ggagctcggc gccgtttcgg
     3721 ccgccgggac cttcgaccag gtgatcctcg tgtttccgga gctggatctc gtcgcggccc
     3781 ggctccagcg cgacgtggag tcgggccgca acggccacta ctggaaccgc gagacgatgg
     3841 cgctcctgcg cgacgtcgtc gagtgagccg aagcgcgacg ccgcgtcagt acgtcagtcc
     3901 gaacccgaac gggaagagcg ggtcgtacgc cgcgtcgccc tcgttgatgg ggtgctggtc
     3961 ggccgagcgc ggccacgtga acgcgagctt gccggtcggc gcgtagtccc cgaagaggac
     4021 gtcggccacg ccctggccct cggttcccgg cagccaggcc gcgacgaccg cgtcgctcgc
     4081 ctcgaggatg tcgccaagga tcaccggccg gcccgagagc acgacggtca cgaccggcgc
     4141 cccggatgcc ttcgcggccg ccaccgcgcg atggtcctcc tcgctgagcc gcagcgccag
     4201 gtcgtcgccg agcatttccg cgtacggccg ctcgccgacc acgaccacga cgacgtcggc
     4261 accctccgag ccggaggcgt ccccggagaa cgtcacctcg gtgccctcgg cgacggccgc
     4321 gcggatcccc tcgataatgg tcgtgccgtc cgtgatgggc ccgagagcgc cctgccactc
     4381 gatggtccag ccgccgctct gcatgccgat gtcatcggcc ccggagcccg cgacgtggat
     4441 gcgctcgacg cctttgacga gcggaagcac gcccccctcg ttccggagca ggacgagcga
     4501 ttcgcgcacg gcccggcggg ccacctcccg gtgggcgtcc gatccgaagc tcgcctcgag
     4561 cgagcggtcg gccgtcggcg accagtcctc gtcgaacagg ccggccgcga acttcacccg
     4621 gaggatccgc cgcacggcgt cgtcgatccg ctccatcgga accgctccct cttcgaccag
     4681 cgacctgagg gtctcgatga actcctggta gcggtccggg accatcacca tgtccatgcc
     4741 ggcgttgatc gactgctcga tgtcgctccg gtaatcgccg ggaagctggt cgatggcccg
     4801 gtaatccgag atcaggaaac cctcgaagcc gagctcctgc ttcaggatct cggtgaggag
     4861 ccgccggctg ccagagacct tctctccgtt ccagctgttg taggagggca tgatggtcgc
     4921 gacgcccgcc tcgatggccg tcacgtagcc ctccatgtgg atgcggcgca gctcttcctc
     4981 ggtcagcctc gtgtcgccct ggtccagcag gagctcctct ccgcgcggga gcccggtgcc
     5041 cggcatcgtc ccgccgtccc cggcgaagtg cttggcgcag gccacgatgc gcagcgggtc
     5101 ggacaggtcc gtgccctgga aaccgcgcac ggctgcggcg cccagttcgg cgacgatggc
     5161 ggggctctcg gagaagccct cgtacgtccg gccccaccgg tcgtcccggg ggaccgccac
     5221 gcacggtgcg aaggtccagt tgatgcccgt ggcccggact tccttcgccg tgatgcggcc
     5281 gacctcctcg acgagcgctg cgttgcgcgt cgcgccgagt ccgacgttgt gggggaagac
     5341 cacggcgccg atgacgttgt tgtgcccgtg gacggcgtcc acgccgtaga gcagcggaat
     5401 gcggagccgt gtctccaggg ccttcgcctg gtaccgctcg gccatggcgc gccacgcgtc
     5461 gaacgtgttc gtgtccggat ccgagccgcc gccgctcagc acggagccca ggaagtagtc
     5521 ccggatgtcg tcgacgctgc gcaaaaactc ctggtcgggc tgggtcatct gcccgatctt
     5581 ttcgtcgagg gtcatctgct cg
//
LOCUS       DBNHQZ010000284         5419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2929485, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000284 DBNHQZ010000000
VERSION     DBNHQZ010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2929485"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC16YU_07650"
     CDS             <1..568
                     /locus_tag="AC16YU_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6065136.1"
                     /translation="VTASRAYKDRIRYGATLHLLRSSVDGLAATALAADVGATMHFEE
                     RLLTLGASVHRLGVVMGSIGSESDDLRPDVRLGIAKRLRYLPVLFTVTAYGLTAWDTD
                     EDGIAFLDRAFRHLLLGAEFQFSDAFQVRFGYDQRRHAALKTGSRLDVAGFSLGAGLH
                     VRRVGIDYAWSSWSSLGGLHRFTVTTRI"
     gene            complement(609..1562)
                     /locus_tag="AC16YU_07655"
     CDS             complement(609..1562)
                     /locus_tag="AC16YU_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6065137.1"
                     /translation="MAPRDPAATASSETAPLVLSLAEIRLRIQDFLREYLRNRSPETV
                     GTYRRSLNEFERWFVLRSGGFRFTPDDVAAYKAYLGEERGLHEVSVSTYLTALRRFCE
                     YLVDIGLLPENPARGVKGNRRPAAHSRKVLTQEEVDRVIETLGTGTEIERRDRAVVYL
                     MLFGGLSEIEIVRADVQDLEQTLMGWYLRVQGKGHTAKDQQVPIDPPVMDAIRLYLDS
                     RGRIRPEEPLFVSHGHRSDGERLNTRSIRSRVNAGLREAGVKRPGITPHSLTHTAALI
                     WLGDGMDVDEVRRRMRHGTLDTTMIYYRSQGLLGGNGEEAS"
     gene            1792..4818
                     /locus_tag="AC16YU_07660"
     CDS             1792..4818
                     /locus_tag="AC16YU_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6065138.1"
                     /translation="MSSIPFHSLRAPGFLLSLLLVAVPSGRASAQDPAGPEVIVGSGG
                     PGETAVGPALEILDEPAPGFALQSLDDPEVSGRFRPLGEDRVVLRGDRWLRLRVRSTL
                     DDEQTMILTFDQGRIPHLTIYAPRDDGSFRISDAGSLTPCNRREVCSRFPSFVVEVRP
                     GPTTTIYLRATAERLFSATPHLRTVQNFAHSLAFDRMTYGAQYGIIAALVLFHLALFL
                     FSRMRATLFLALFLAAAGLYGGLVEGTLIRALPLEITGFDREVLKRVLEALAFLFLLR
                     FGSAHLLDDAPGWVRTRQAIRFLTAAAIVAVPVVAVTPMDGIWVNGLVGVLVLSTLLY
                     LSMLTSREASWPGRLHGFGILVLVAGFGLYALSVYAVARSTSDLIIAVTLPVDLYGLP
                     TTVALFAVFGTMAVVRELYRVRRERDVAQASTLQQLLDDKVLREQWNVHLEREIEKRT
                     GELERAKTQLEHQNFELRRHERQLEGQAQQLQELQAHKSRFFAYISHEFRTPITLIRA
                     RVEDIVSDFAEAKPQDTRRRLDTVLQNTYRLEHLVDEVLDLSRVEEGTAVIRASRSDF
                     AAFARRVVEQHVQLAGQRGIDLEYPSQAEGIDAYFDEEMMTKVITNLLSNAIKFSDRG
                     GRIEVWVSESSPEPDAGYVRVDVVDQGPGIHRDDLPHVFERFFQSVRPGTQPRVGHGL
                     GLSLTKHLVELHGGEVRVASDEGEGSTFSIFLPKGRAHLTDAEIVPERPSRAAATVVE
                     PDLELMRGAGGQPRLLIVEDEPELLALLREHFEKEYDVATAIDGQDGLKAAREVRPEL
                     IITDVLMPRMDGLEMLAAIRRDAQLENTPVLILSAKASIEDRLTAYQAAADGYISKPF
                     DMTELRIRVRNIIRSRRALMFSRAEAAISVDGRDLPADSVDATFIASARAAVMAHLDQ
                     DDFDVEAFSGALNMSSRTLHRRMKEIVDMAPAEFVRTIRLQAARHLIEQNAVGTVSEA
                     ATRVGFKSASYFARLYREVFGESPADLLNSETDTPH"
     gene            5139..>5419
                     /locus_tag="AC16YU_07665"
     CDS             5139..>5419
                     /locus_tag="AC16YU_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065139.1"
                     /translation="MGMQQIILLALAVIIVGIATVYAIEAFTLNRTRSNMDALIQDAV
                     TIGHEMQQWKSRPTQFGGQPCEATVPPTGVCDDPNPRTIPEDYSMFTWD"
BASE COUNT          826 a         1929 c         1796 g          868 t
ORIGIN      
        1 cgtcacggcg tcccgcgcgt acaaggaccg catccgctac ggcgccacgc tgcacctctt
       61 gcgctcgtcc gtcgacgggc tcgccgcgac cgcgctcgcg gccgacgtcg gggcgaccat
      121 gcacttcgag gagcggctgc tgacgctcgg cgcgagcgtc caccggcttg gcgtcgtcat
      181 gggatccatc ggctcggagt cggacgacct ccggccggac gtccgcctgg gcatcgcgaa
      241 gcggctgcgc tacctgcccg tgctcttcac cgtgaccgcc tacgggctca ccgcatggga
      301 cacggacgaa gacggaatcg ccttcctgga ccgggcgttc cgccacctgc tcctcggcgc
      361 cgagttccag ttctcggacg ccttccaggt gcgcttcggc tacgaccagc gccggcacgc
      421 ggcgctcaag accgggtcga ggctggatgt cgcgggcttc agcctgggcg cggggctcca
      481 cgtgcggcgc gtgggcatcg actacgcgtg gtcgtcctgg tcgtcgctgg gcggactgca
      541 ccggttcacg gtgacgacgc ggatctgaag gcgcctgcga cgccggacgt cgctcgactc
      601 cgcgccgcct acgacgcctc ctccccattt cccccgagca gcccctggct ccggtagtag
      661 atcatggtcg tgtcgagcgt gccgtgccgc atgcggcggc gcacctcgtc cacgtccatg
      721 ccgtcgccca gccagatgag cgcggccgtg tgggtcaggc tgtgcggcgt gattccgggg
      781 cgcttgacgc ccgcctcgcg aaggcccgcg ttcacccggc tgcggatgga gcgcgtgttc
      841 aggcgctcgc cgtccgagcg gtggccgtgc gagacgaaga gcggctcctc gggccggatg
      901 cgccctcggc tgtccaggta caggcggatg gcgtccatga ccggcgggtc gatgggcacc
      961 tgctggtcct tggccgtgtg ccccttgccc tgcacgcgaa ggtaccagcc catgagcgtc
     1021 tgctccaggt cctgcacgtc cgcccggacg atctcgatct cggagagccc gccgaagagc
     1081 atcaggtaga cgaccgctcg gtcgcgccgc tcgatctcgg tcccggtgcc gagggtctcg
     1141 atcacccggt ccacctcttc ctgcgtgaga accttgcgcg agtgcgcggc cggccgccgg
     1201 ttgcccttca cgccgcgcgc cgggttctcc gggaggagcc cgatgtccac caggtactcg
     1261 cagaagcggc ggagcgccgt caggtacgtg gagacggaca cctcgtgcag gccccgctcc
     1321 tcgcccaggt aggccttgta ggcggcgacg tcgtccggcg tgaagcggaa gccgccgctg
     1381 cgcagcacga accagcgctc gaactcgttc agcgaccggc gataggtgcc cacggtctcc
     1441 gggctccggt tgcgcaggta ctcgcgcagg aaatcctgga tgcggagccg gatttcggcc
     1501 agcgacagga cgagcggcgc ggtctcggag gacgcagtcg cggcggggtc gcgcggggcc
     1561 atgggcggac ggtgcgtgct gacggcatta acggaacgtc gggggcccac gcacgcgcag
     1621 gcggcccgcc gccctcggga atcaaaggaa cggcgcatcc gccgctttgt caataacggc
     1681 attcaagccc gcgccaggat gccgatacct cgtgttcgcc attgcttctt acccccggat
     1741 gacggtactt gcgtggttca gatgcgagtg ctccgtaagt cccccgtttc gttgtcctcc
     1801 atcccgttcc atagcctgcg ggcgcccgga ttcctcctgt cgctgctgct cgtcgcggtc
     1861 ccgtccgggc gcgcctcggc gcaggacccg gccgggccag aggtcatcgt tgggtccggt
     1921 ggaccgggcg agaccgccgt tggacccgcc ctggaaatcc tggacgaacc cgctcccggt
     1981 ttcgccctgc agtccctcga cgaccccgag gtctccgggc gcttccggcc cctgggagag
     2041 gaccgcgtcg tgctccgggg cgaccggtgg ctccggctcc gggtccgctc cacgctggac
     2101 gatgagcaga ccatgatcct gacgttcgac cagggtcgca tcccgcacct cacgatctac
     2161 gcgccgcgcg acgacggctc gttccgcatc agcgacgcgg gatccctgac gccctgcaac
     2221 cggcgcgagg tatgcagccg gttcccgtcc ttcgtggtcg aggtccgccc gggacccacg
     2281 acgacgatct acctgcgggc gacggccgaa cggctgtttt cggccacgcc tcatctgagg
     2341 acggtccaga atttcgcgca ttcgctcgcc ttcgaccgga tgacgtacgg cgcccagtac
     2401 ggcatcatcg ccgcgctggt gctgttccac ctcgcactgt tcctgttcag ccgcatgcgg
     2461 gccacgcttt tcctggccct gttcctcgcg gcggccggcc tgtacggggg cctggtcgag
     2521 ggcacgctga tccgggccct gccgctcgag ataacgggtt tcgaccgcga ggtgctcaag
     2581 cgggtgctcg aagcgctcgc gttcctgttc ctgctgcgct tcggatcggc ccacctgctc
     2641 gacgacgcgc cgggctgggt gcgcacgcgg caggcgatcc gcttcctgac cgccgccgcc
     2701 atcgtcgccg tgcccgtcgt cgcggtcacc ccgatggacg ggatctgggt gaacggcctc
     2761 gtgggcgtcc tggtgctcag caccctcctg tacctctcca tgctgacgtc gcgggaggcg
     2821 tcgtggccgg gccggcttca cggcttcggg atcctggtgc tcgtcgcggg cttcggcctg
     2881 tacgcgctga gcgtatacgc ggtagcccga tccacgtcgg accttatcat cgccgtcacc
     2941 ctcccggtgg acctgtacgg actgcccacc acggtcgccc tcttcgcggt cttcggcacc
     3001 atggcggtgg tccgcgagct gtaccgcgtg cggcgcgagc gcgacgtggc ccaggcgtcc
     3061 accctgcagc agctcctcga cgacaaggtg ctgcgcgagc agtggaacgt gcacctggag
     3121 cgggagatcg agaagcgcac aggcgagctc gagcgcgcga agacgcagct cgagcaccag
     3181 aacttcgagc tccgccgcca cgagcggcag ctcgaggggc aagcgcagca gctgcaggag
     3241 ctccaggcgc acaagagccg cttcttcgcc tacatctcgc acgaattccg gacgcccatc
     3301 acgctcatac gcgcgcgcgt ggaggatatc gtgagcgatt tcgccgaggc gaagccccag
     3361 gacacgcgcc gccgcctgga cacggtcctg cagaacacgt accgcctgga gcacctggtg
     3421 gacgaggtgc tggacctctc gcgcgtcgag gagggcacgg ccgtgatccg ggcgtcccgg
     3481 tccgacttcg ccgcgttcgc acggcgggtc gtggagcagc acgtgcagct cgccggccag
     3541 aggggcatcg acctcgagta cccgtcgcag gccgagggca tcgacgccta cttcgacgag
     3601 gagatgatga ccaaggtgat caccaacctc ctctcgaacg ccatcaaatt ctcggaccgc
     3661 ggcgggcgga tcgaggtatg ggtgagcgaa tcctcgcccg agcccgacgc cgggtacgtc
     3721 cgcgtggacg tcgtggacca gggccccggc atccaccggg acgacctgcc ccacgtgttc
     3781 gagcggttct tccagtcggt gcggccgggc acccagccgc gcgtcgggca cggcctgggg
     3841 ctctcgctca cgaagcacct ggtggagctg cacggcggcg aggtgcgcgt ggcgagcgac
     3901 gaaggcgagg gctccacgtt ctcgatcttc ctgcccaagg gccgggccca cctcaccgac
     3961 gccgagatcg tcccggagcg cccatcccgc gcggcagcga ccgtcgtgga gcccgacctg
     4021 gagctgatgc gcggcgccgg gggccagccc cgcctgctca tcgtggagga cgagcccgag
     4081 ctcctggcgc tcctccggga gcatttcgag aaggaatacg acgtcgcgac cgctatcgac
     4141 gggcaggacg gcctgaaggc ggcgcgcgag gtgcggcccg agctcatcat cacggacgtg
     4201 ctcatgccgc ggatggacgg cctggagatg ctggccgcta tccgccgcga cgcccaactc
     4261 gagaacacgc ccgtcctgat cctctcggcc aaggcgtcca tcgaggaccg gctcacggcc
     4321 taccaggcgg ccgccgacgg ctacatctct aagcccttcg acatgaccga gctgcgcatc
     4381 cgcgtgcgca acatcatccg ctcgcgccgg gcgctcatgt tctcgcgcgc cgaggccgcg
     4441 atctcggtcg acggccgcga cctgccggct gacagcgtgg acgcaacctt catcgcgtcc
     4501 gcccgcgcgg ccgtgatggc gcacctggac caggacgact tcgacgtgga ggcgttctcg
     4561 ggcgcgctga acatgagctc gcgcacgctc caccggcgga tgaaagagat cgtggacatg
     4621 gcgccggccg agttcgtccg cacgatccgc ctgcaggccg cgcgccacct gatcgaacaa
     4681 aacgcggtgg gcacggtaag cgaagccgct acccgcgtgg gcttcaagag cgcctcgtat
     4741 ttcgcccggc tgtaccgcga agtgttcgga gaatcgcccg cggacctcct gaattccgaa
     4801 actgacaccc cccactgaca agtcggcagt cacaaatccg ccgacagggc tcacaaaccc
     4861 gccacagccc aggcccgaga cgaccgccct cggagattta cgttaatgct tttcggacac
     4921 ctgtcgatct ttttggacac gatccgtcac ggacgtgcca atgtggcgca ccgtcgtagg
     4981 atcggctgaa aagcgcccgt aatcttgaca tcgcccgcca ggcccgtcgc ggcccgcttt
     5041 ccgaacgcgt acctctcacc acgcttccgc ggatcgcgac gtgactcgca ggcgggatga
     5101 acgaaacctt acctaactac ctgaagaggt aaacgaacat gggaatgcag caaatcatcc
     5161 tgctcgccct ggcggtcatc atcgtcggca tcgcgacggt gtacgcgatc gaggctttca
     5221 cgctcaaccg cacgcggtcg aacatggacg ccctgatcca ggacgcagtg accatcggtc
     5281 acgagatgca gcagtggaag tcgcggccaa cgcagttcgg cggacagccc tgtgaggcta
     5341 ccgttccccc gacgggcgtc tgtgacgacc cgaacccgcg cacgattccc gaggactaca
     5401 gcatgttcac gtgggatca
//
LOCUS       DBNHQZ010000285        13094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2117930, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000285 DBNHQZ010000000
VERSION     DBNHQZ010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2117930"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16YU_07670"
     CDS             <1..730
                     /locus_tag="AC16YU_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6065140.1"
                     /translation="FEDGRYVWPVYPEYDIYTANQDGSDVQPLITGPGYDAEATVSPD
                     GRWVVFTSTRSGDLELWRYDLKSGEVLQLTDGLGYDGGAFFSHDSQKIVWRADRPGEE
                     EAEVYRSLLERNLVQPSELDIWVANADGSEAVQVTNLEGANWAPYFHPDDRRILFTSN
                     HHTMAEGGREFDIFMVNLDGSDLVRITHSGTFDAFPMFSPDGARLVFASNRNASRTPT
                     RDTNIFVAEWVDQPTDADLGFGEE"
     gene            846..1784
                     /locus_tag="AC16YU_07675"
     CDS             846..1784
                     /locus_tag="AC16YU_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIR6065141.1"
                     /translation="MARFAPWLLLAASGFAAACSPDAGPPADADGRLRVVATTSMIAE
                     LAARVGGDDVVVTGLMGPGVDPHLYKASEGDVSRMAEADLVVYNGLHLEGKMVDIFEQ
                     MESRGMATVAIASGIAADRLISSELFVGSHDPHVWFDVALWREAGRVVADRLAELRPG
                     AADAFETRYREYAAELDSADAYVRDRAGSLPPGSRVLVTSHDAFSYFGRAYGFEVRGL
                     QGLSTAAEAGTADVQELVDFVVDRRVPAIFFETSVSPRGIEAVLAAVRDRGFDVRLGG
                     TLYGDALGSPGSEAGTYAGMMRSNIDTIVNALAPPS"
     gene            1784..2533
                     /locus_tag="AC16YU_07680"
     CDS             1784..2533
                     /locus_tag="AC16YU_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR6065142.1"
                     /translation="MSEAAVDIRDLTVAYRDRPVLWDIDLTVQPGVLMAVVGPNGAGK
                     TTLIKAVLGLVPRAAGDVLIHGLPYAEARSRVAYVPQRGSVDWDFPTHVLDVVTMGLY
                     GRLGWLRRPGRAERALALEALDQVGMADLAGRQISQLSGGQQQRVFLARALVQDADVC
                     FMDEPFQGVDATTERAIVGLLRDLRARGRTVVVVHHDLQTVGEYFDEVFLLNVRRIAA
                     GRVEDVFTEENLRLTYGGRVAFLKSPTGQMA"
     gene            2530..3441
                     /locus_tag="AC16YU_07685"
     CDS             2530..3441
                     /locus_tag="AC16YU_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR6065143.1"
                     /translation="MMDLLGDLFSDYTLRTVALGAAVLGMTSGALGAFAVLRRQSLLG
                     DAISHAALPGIALAFILTGSKAPLVLVLGAALSGWIGTLLILQVTERTRIPYDTALGL
                     MLSVFFGIGLVLLTWIQRQPDANQAGLESYLFGQAAALVERDVVTMATLGAVSLAALA
                     AGWNGFKVLAFDRDFGAAVGIPVRRLDVALTGLLVVSIVIGLQTVGVVLMSAMIIAPA
                     AAARQWTDRLGRMVLLAGAFGAAAGVTGAVLSAVRPALPTGPTIVLCAGLVVIVSLVA
                     APGRGVLWQRLHAWRRDRRNRTDSGAP"
     gene            3438..4550
                     /locus_tag="AC16YU_07690"
     CDS             3438..4550
                     /locus_tag="AC16YU_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR6065144.1"
                     /translation="MSGAQLEVQLVAVVVAVACALPGSFLVLRRMAMMSDAISHSILP
                     GIVVGFLLTGDLGSPWLVGGAAATGLLTVALVEGIQRTGLVREDAAIGIVFPVLFSAG
                     VILIAVYAGDVHLDMDAVLLGEIAFAPFDRFTAGGTDLGPRSLWIMGGLLVIDAAFIA
                     AFFKELKLATFDAGLAAALGFMPGLLHYGLMGLVSLTAVGAFDAVGSILVVALMVGPP
                     AAAWLLTDRLPVMLPLAAGIGALCAVGGYWMARALDASIGGSMATAVGIAFALAALFA
                     PGRGLVAGARRRSRQRWTFAERMLAVHLLSHEGSPEAERECRPEHLQEHLRWDGTFAT
                     TVVRKAIGSGLVRRSGPVLELTPAGREFAGRSITEL"
     gene            complement(4560..7016)
                     /locus_tag="AC16YU_07695"
     CDS             complement(4560..7016)
                     /locus_tag="AC16YU_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR6065145.1"
                     /translation="MIGAIGKLFILVGFVSVILAGIAYFRAAQLETSARDWRRIGRGA
                     WLVSTVSAFIAYGVLFYLSLTHQFQYAYIYSHTSRALGLDYLISACWAGQEGSFMLWI
                     VLNGLVGFALMKWARGYESPTMAVVALCQFFLFSMVAGLKLGDVTIGSSPFMMLAEKF
                     PDAPMLQAGLIPTDGQGLNDLLQNYWMVIHPPTLFVGFAAMIVPFAFAVTALWKKQYT
                     QWVRPALPWALFASMILGLGIAMGGYWAYVTLSFGGYWAWDPVENSSLVPWIVGIAAI
                     HTMIVQKRSGRSHKAALLLNVLAYMLVIYSTFLTRSGILGDISVHSFVDLGLYNQLLL
                     WILTMGVISVGLYVKRYRQLPSPKNEPEFLSREFMIFAGAILLSCVALVVILGTSAPI
                     IGRIFRSSPSTVPIEFYNKWTLPLAVMFTFLAGLGQLFWWNRMSAESVNKVLFRPVVF
                     AVAATVAVLLFTPFVEASAVPQAMAAASMPEGAQSAALLVGIGTFWATYGQGILLLLL
                     LFVAFFALYGNGEVAWRIARGNPRMAGGAIAHVGLAIMILGIISSSGFSNPLGRNAGV
                     RMGDSRDNFVLSRGETRVVSGYTVTYRTQEVNEEGRPQYVLDFTDPDGRAFTVAPVVY
                     KSNKDQWIQHPDVTVFPEKDLFVAVSPNVMFGSSSDSESGTLTLARGDSVLIGEGAYA
                     VKFVEFDMSVDHEQVLPSAEVAVGARIDVFNLATGESRQLRPVYQIAEDRSVHLVSDS
                     VPEWNLTVSFAGMNVDNGAITLQVEGIQVAPEDWLVVQAYEKPMISLVWIGLILLTFG
                     FGVSIVRRAKDEQYGSERQA"
     gene            complement(7219..8919)
                     /gene="rny"
                     /locus_tag="AC16YU_07700"
     CDS             complement(7219..8919)
                     /gene="rny"
                     /locus_tag="AC16YU_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR6065146.1"
                     /translation="MDPLTVGFLVALVAAVALGTGIILDRFILKRFGLNRLAEAREKA
                     HHLLADAEKEIEALHEQRIAKAEEDLRRREQELQEEAEETRRNWRRARQKLEKRNERL
                     SRIAHRIGEQESLLQEASDVIDLLRVEAEEQKAATATVLRRAEVLFGQVRERSEGVDA
                     ARAEIDRQLAALEDRTRRLDETIGEYVGRLEQVSGLPREEARRLLIDQMTDEARLDAA
                     ALVKEVRDEAKLQANREARKVVLSAIQRTAASHTIENTVSVVNLASDDMKGRIIGREG
                     RNIRAFEAATGIEVIVDDTPEAVILSGFNPVRREIARISLNRLIQDGRIHPARIEEIV
                     DRTRTEVEDEILEIGEQTVIELNLSRVHPELVRMVGRMRFRSSYGQNLLAHSIEVAGL
                     ASRMAAELDLDAGLARRAGLLHDIGKVAEEDIEQPHALVGMELCRRFKEHEEVCNAVG
                     AHHDEIPMTSLLSPLVQAADAISGSRPGARREALESYIRRLENLEALAASFPGVDRVY
                     AIQAGREIRVLVNHDRISDALAEQLAVDISKRIQAEMQYPGQIKVTVIREVRSVSYAK
                     "
     gene            complement(9389..9682)
                     /locus_tag="AC16YU_07705"
     CDS             complement(9389..9682)
                     /locus_tag="AC16YU_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR6065147.1"
                     /translation="MQKSIRINIGGRDYSLRVREENEVLTRELAHYVDAKLRAFKDAH
                     PDQSDTTAAVISAMAIAEELFMARSGSEDRGDSIAVALDELERELATALLSKA"
     gene            complement(9682..10083)
                     /locus_tag="AC16YU_07710"
     CDS             complement(9682..10083)
                     /locus_tag="AC16YU_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065148.1"
                     /translation="MSETPERDMPEAGGPSQGDMFSDASDPDSIDASRTGRRPLSMKT
                     ERALERLRDRISLTARELRRLREENVRLHRQVEELQTRGLGEVEGTPVVFTEDAAALR
                     SKVERFIESIDLHLAGRSVTESGDGSGSPAP"
     gene            complement(10152..12629)
                     /gene="pheT"
                     /locus_tag="AC16YU_07715"
     CDS             complement(10152..12629)
                     /gene="pheT"
                     /locus_tag="AC16YU_07715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6065149.1"
                     /translation="MKISTRWLRTYVDHDWSAEELADRLTLCGLEVESVEAVGPTLDG
                     VVVGRVLGVRAHPEADRLTLCDVDTGNGEAVQIVCGAPNVAAGQVVPVATVGTTLHLP
                     DRDDPGVRVPVTLRSARIRGEVSHGMICAEDELGLSDDHSGIMVLEEHGRPGESFAAY
                     LERSGYPATDTVLDVAVTPNRPDATSHLGVARDVAALADRPLRRPDVALPREGGEAAL
                     AFSVDIEAPDGCNRYVGLLVRGVTIAESPAWLRQRLAAIGLRPRNNVVDITNFVMHEC
                     GQPLHAFDFDQLAGHRIVVRRTAAEGPFTTLDGKVRTLPKGTLMIADGEREVAVAGVM
                     GGENSEVTQATTDVLIESAYFDPSDIRRTAKALQLPSDASYRFERGVDPAGQVWAAAR
                     AAALMVQIAGGTLVDGMVDAHPVPAERRTLHVRSARIAAVLGVDVPAADAERLLRAIG
                     FEPRRRDDGLSVTVPTWRPDVEREIDVIEEIARLHGLDRIPEPTHSRLPNVLPTSPAE
                     QPVRDRAAQILSGAGFREICTNSMLPPAVATLFAHPVLRGGVAVGEAVQTLNPISTEM
                     AALRPSLLPGMLQVLAHNLNHGQAGLRMFEIGVVMDRFDAPGAPVAGYRERTSLLVAV
                     TGRISEPGWDTKGRSADVFDLRGHVEALFEGLGVSGYRLDPVREPTPLTRYHLVIRLG
                     DRPIGSLAAVGRDVLDRFDLRGEVFFAEFDLGAVADGLAGRGWTRYRDVTRFPVVDRD
                     IAVVVDAGVAVGPLLDAIREAGAPLLRRTGVFDLYRDERLGPGRKSVAFSMRFASDRT
                     LRDAEVDEVVERIVRVLGERYGAVLRT"
     gene            complement(12654..>13094)
                     /locus_tag="AC16YU_07720"
     CDS             complement(12654..>13094)
                     /locus_tag="AC16YU_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA ligase subunit PheS family protein"
                     /protein_id="HIR6065150.1"
                     /translation="VPGRVYRNEALSYKSYCLFHQVEGLYVDEGVSMADLKEVLHTFA
                     RALFGDDVRMRFRPSFFPFTEPSAEVDIWWRDESRPEGGRWLEILGCGMVDPAVLEAV
                     DVDPERYTGYAFGMGVERIAMLRYDISDIRILYENDVRFLEQFT"
BASE COUNT         1910 a         4500 c         4614 g         2070 t
ORIGIN      
        1 gttcgaggac ggccgctacg tgtggccggt ctacccggag tacgacatct acacggcgaa
       61 ccaggacggc agcgacgtgc agccgctgat caccggtccg ggctacgacg ccgaggccac
      121 cgtgtcgccg gacggccgct gggtggtgtt cacgtcgacg cgctcggggg acctggaact
      181 gtggcgctac gacctgaaga gcggcgaggt gctccagctc acggacggtc tcggatacga
      241 cggcggggcg ttcttctcgc acgactcgca gaagatcgtc tggcgcgccg accgcccggg
      301 ggaagaggag gccgaagtct accggtcgct cctcgagcgg aacctggtcc agccgagcga
      361 gctcgacatc tgggtggcga acgcggacgg ctcggaggcc gtgcaggtca cgaacctgga
      421 aggcgcgaac tgggcgccgt acttccatcc ggacgaccgg aggatcctct tcacgtccaa
      481 tcaccacacg atggcggagg ggggacgcga gttcgacatc ttcatggtga atctggacgg
      541 ttcggacctg gtccgcatca cgcattcggg cacgttcgac gcgttcccga tgttctcgcc
      601 cgacggcgcg cgcctggtct tcgcctccaa ccgcaacgcg tcccggacgc ccacgcggga
      661 tacgaacatc ttcgtggcgg aatgggtgga ccagccgacg gacgcggacc tgggcttcgg
      721 cgaggagtga tcggctcgag atgcgcgctg cggcgggcgc ggccgacccg gtgcgatttt
      781 agtcgcaact aaactgtgaa gatcggatat aacgggctgt accgattccg agaggtcccc
      841 cggacatggc ccgtttcgct ccctggctgc tcctggcagc ctccggcttc gccgcagcct
      901 gcagcccgga cgccggcccg cccgccgacg ccgacggccg gctccgcgta gtcgccacga
      961 ccagcatgat cgcggagctc gccgccaggg tcggaggcga cgacgtggtc gtgaccgggc
     1021 tgatggggcc cggggtggat ccgcatctct acaaggcatc cgaaggggac gtctcccgga
     1081 tggcggaggc ggacctggtc gtatacaacg ggctgcacct ggaggggaag atggtggaca
     1141 tcttcgagca gatggagtcc cgcggcatgg ccacggtggc gatcgcctcc ggcatcgcgg
     1201 cggatcggct catctcgtcc gagctcttcg tcggcagcca cgatccccac gtctggttcg
     1261 acgtcgccct gtggagggag gcgggccgcg tggtggcgga ccggctcgcg gaactcaggc
     1321 ccggggcggc cgatgcgttc gagacgcgct accgggagta cgcggccgag ctggactcgg
     1381 cggacgccta cgtccgggac cgggccgggt cgctcccgcc ggggagccgc gtgctggtca
     1441 cctcgcacga cgcattctcg tatttcggga gggcctacgg attcgaggtc cgcggcctgc
     1501 agggcctctc gacggcggcc gaggcgggta cggccgacgt gcaggaactg gtcgatttcg
     1561 tcgtcgaccg gcgtgtcccg gcgatcttct tcgagacgtc cgtgtcgccc cggggcatcg
     1621 aggcagtcct cgcggcggtc cgggaccggg gcttcgatgt ccgcctcggc gggacgctct
     1681 acggggacgc cctcggctcc ccgggatccg aggcgggcac gtacgcgggc atgatgcggt
     1741 cgaacatcga cacgatcgta aacgcgctcg cgcctccctc gtgatgtccg aagcggccgt
     1801 cgatatccgc gacctcacgg tcgcgtaccg ggaccgtccc gtgctgtggg acatcgacct
     1861 cacggttcag ccgggggtgc tcatggcggt cgtcggcccg aacggcgcgg gaaagaccac
     1921 cctcatcaag gccgtgctcg gcctggtccc gcgcgcggca ggcgacgtcc tgatccatgg
     1981 cctcccgtac gcggaggcgc gcagccgcgt ggcctacgtt ccgcagcggg ggagcgtgga
     2041 ctgggacttt cccacgcacg tgctcgacgt cgtcaccatg gggctttacg gccggctggg
     2101 ctggttgcgg cgtcccgggc gggcggagcg ggcgctcgcc ctcgaggcgc tcgaccaggt
     2161 gggcatggcc gacctggcgg ggcgccagat ctcgcagctc tcgggcgggc agcagcagcg
     2221 cgtgttcctc gcgagggccc tcgtgcagga cgcggacgtc tgtttcatgg acgaaccctt
     2281 ccagggcgtg gatgccacca cggagcgggc catcgtgggc ctgctgcgcg acctgcgcgc
     2341 ccgcgggcgc acggtcgtcg tcgtccacca cgacctgcaa accgtgggcg agtacttcga
     2401 cgaggtgttc ctgctcaacg ttcgacgcat cgcggcgggc cgggtcgagg acgtgttcac
     2461 cgaggagaac ctgcgcctga cctacggtgg gcgcgtggcg ttcctgaagt cgccgacggg
     2521 ccagatggca tgatggatct cctcggcgac ctgttctcgg actacacgct gcggacggtg
     2581 gccctgggcg cggcggtgct gggcatgacc agcggggcac tcggggcctt cgccgtgctg
     2641 cgccggcaga gcctgctcgg cgacgccatc tcgcacgcgg ccctgcccgg catcgcgctg
     2701 gccttcatcc tgacgggcag caaggcgccc ctggtcctgg tgctcggggc agccctctcg
     2761 gggtggatcg gcacgctgct catcctgcag gtcaccgaac gcacccggat tccgtacgac
     2821 acggccctgg ggctcatgct gtcggtcttc ttcggcatcg ggctcgtgct cctcacgtgg
     2881 atccagcggc agccggacgc caaccaggcg ggcctcgagt cgtacctgtt cggccaggcg
     2941 gccgccctcg tcgagcgcga cgtcgtgact atggccacgc tcggcgcggt ctcgctggcc
     3001 gccctcgccg cgggctggaa cgggttcaag gtgctcgctt tcgaccggga tttcggggcg
     3061 gccgtgggca ttccggtccg ccggctggac gtcgccctga cggggctcct ggtggtttcc
     3121 atagtgatcg gccttcagac ggtcggggtc gtcctcatga gcgcgatgat catcgcgccc
     3181 gcggcggctg cgcgccagtg gacggaccgg ctcggccgga tggtcctgct ggcgggcgcc
     3241 ttcggggcgg ccgccggcgt cacgggcgcc gtcctgagcg cggttcggcc cgccctgccg
     3301 acgggaccga ccatcgtgct ctgcgcgggt ctcgtcgtca tcgtgtcgct cgtcgccgcg
     3361 ccgggccgcg gcgttctctg gcagcggctg catgcctggc ggcgggatcg gagaaaccgc
     3421 acggacagcg gtgccccatg agcggcgcgc agctcgaagt ccagctggtg gccgttgtcg
     3481 tggcggtggc ctgcgcgctg ccgggatcgt tcctggtgct gcgccgcatg gccatgatga
     3541 gcgatgccat cagccactcg atcctgccgg gcatcgtcgt cggcttcctg ttgaccgggg
     3601 acctgggctc tccgtggctg gtcgggggcg ccgccgctac ggggctcctg accgtggccc
     3661 tggtggaggg catccagcgc acgggcctgg tgcgcgagga tgcggccatc gggatcgtgt
     3721 ttccggtcct cttcagcgcg ggcgtgatcc tcatagccgt ctacgcgggc gacgtgcacc
     3781 tggacatgga cgccgtgctg ctcggcgaga tcgccttcgc tcccttcgac cgcttcacgg
     3841 caggcggcac ggatctcggt ccccggtcgc tgtggatcat gggtggtctg ctggtcatcg
     3901 acgcggcctt cattgcggcc ttcttcaagg agctgaagct cgcgaccttc gacgccggcc
     3961 tcgcggcggc cctgggcttc atgcccgggc ttctgcacta cggcctgatg ggcctggtct
     4021 cgctgaccgc cgtgggcgcc ttcgacgcgg tgggatcgat tctggtggtc gcgctcatgg
     4081 tcggtccgcc ggccgcggca tggctcctca ccgaccggct gcccgtgatg ctgccgctcg
     4141 ccgccggcat cggcgcgctg tgcgccgtgg gcgggtactg gatggcccgt gccctcgacg
     4201 catcgatcgg gggctcgatg gccacggcgg tgggcattgc cttcgcgctc gcggccctgt
     4261 tcgcccccgg ccgcggtctc gtcgccggcg cacgtcgccg gagccgccag cggtggacgt
     4321 tcgcggagcg gatgctggcc gtgcaccttc tctcgcacga gggctctccc gaggcggaac
     4381 gcgaatgccg cccggagcat ctccaggagc acctgcgctg ggacgggacg ttcgccacga
     4441 ccgtcgtgcg gaaagcgatc gggtccggcc tggtccgccg gtccggcccg gtgctcgaac
     4501 tcacgccggc cgggcgcgag ttcgccggcc ggtccatcac cgagctctga cgcgcccgct
     4561 caggcctgcc gctccgatcc gtactgctcg tccttcgcgc ggcgcacgat cgagacgccg
     4621 aacccgaacg tcaggaggat caggccgatc cagacaaggc tgatcatcgg cttctcgtag
     4681 gcctgcacca cgagccagtc ctcgggcgcg acctggatgc cctcgacctg gagcgtgatg
     4741 gcgccgttgt ccacgttcat gcccgcgaac gagacggtca ggttccactc gggcaccgag
     4801 tcgctgacca ggtgcacgga gcggtcctcc gcgatctggt agaccgggcg cagctgccgg
     4861 ctctcgcccg tcgccaggtt gaagacgtcg atgcgggcgc cgaccgccac ctcggcggac
     4921 ggcaggacct gctcgtggtc gaccgacatg tcgaattcca cgaacttcac ggcgtacgcc
     4981 ccctcgccga tgagcaccga gtcgccgcga gcgagcgtca gcgtcccact ctccgagtcc
     5041 gacgaggagc cgaacatcac gttcggggac acggcgacga agaggtcctt ctccgggaag
     5101 acggtcacgt ccggatgctg gatccactgg tccttgttgc tcttgtacac gaccggtgcg
     5161 accgtgaagg cgcggccgtc cgggtccgtg aagtcgagca cgtactgcgg tcggccttcc
     5221 tcgttcactt cctgcgtccg gtacgtgacc gtgtagccgc tcacgacccg cgtctcgccg
     5281 cgcgagagta cgaagttgtc ccggctgtcg cccatccgga cgccggcgtt gcgcccgagc
     5341 ggattgctga acccggacga cgaaatgatg cccaggatca tgatggcgag gccgacgtgg
     5401 gcgatcgcgc cgccggccat gcgcgggttg ccacgggcga tccgccaggc gacctcgccg
     5461 ttgccgtaca gggcgaagaa ggcgacgaag agcaggagca gcagcaggat gccctgcccg
     5521 tacgtggccc agaacgtgcc gatccccacc agcagggccg ccgactgcgc gccctcgggc
     5581 atcgaggcgg ccgccatggc ctggggcacc gccgacgcct cgacgaacgg tgtgaacagc
     5641 agcacggcga cggtcgccgc cacggcgaag accaccgggc ggaacagcac cttgttcacc
     5701 gactcggccg acatgcgatt ccaccagaac agctggccga gaccggccag gaaggtgaac
     5761 atcacggcga gcggcagcgt ccacttgttg tagaactcga tcgggaccgt ggacgggctc
     5821 gagcggaaga tccggccgat gatgggcgcg ctggtgccca ggatcacgac gagcgcgacg
     5881 cagctcagca ggatggcgcc cgcgaagatc atgaactcgc gcgagaggaa ttcaggctca
     5941 ttcttcgggc tgggcagctg ccggtaccgc ttcacgtaca gtccgacgct gatgacgccc
     6001 atcgtcagga tccacagcag cagctggtta tacagcccga ggtccacgaa ggagtgcacc
     6061 gagatgtcgc ccaggatgcc cgaccgcgtc aggaacgtcg agtagatgac cagcatgtag
     6121 gccaggacgt tcagcaggag cgccgccttg tggcttcgtc cgctgcgctt ctgcacgatc
     6181 atcgtgtgga tggcggcgat gccgacgatc caggggacca gggacgagtt ctcgacggga
     6241 tcccaggccc agtagccgcc gaacgacagc gtgacgtagg cccagtagcc gcccatcgcg
     6301 atgccgaggc ccaggatcat gctggcgaag agcgcccagg gcagcgcggg gcgcacccac
     6361 tgcgtgtact gcttcttcca gagcgccgtc accgcgaagg cgaacgggac gatcatggcg
     6421 gcgaagccca cgaagagcgt cggcgggtgg atgaccatcc agtagttctg caggaggtcg
     6481 ttcagcccct gtccgtccgt ggggatgagg cccgcctgca gcatcggcgc atccgggaat
     6541 ttctcggcca gcatcatgaa gggcgacgac ccgatggtca cgtctccgag cttgagcccg
     6601 gcgaccatcg aaaagaggaa gaactggcag agcgcgacga cggccatcgt gggcgactcg
     6661 tagccgcggg cccacttcat gagggcgaat ccgaccaggc cgttcagcac gatccagagc
     6721 atgaacgagc cttcctgccc cgcccagcac gcggaaatga ggtagtcgag gcccagcgcc
     6781 cgcgaggtgt gcgagtagat gtaggcgtac tggaattggt gcgtcaggct caggtagaac
     6841 agcaccccgt acgcgatgaa cgccgacacg gtggacacca gccacgcccc ccggccgatg
     6901 cggcgccagt cccgggcgct ggtctcgagc tgcgccgccc ggaagtaggc gatgccggcc
     6961 aggatgaccg agacgaatcc gacaaggatg aatagcttgc ctatggcgcc gatcatgtcg
     7021 tcttcaggtt gggtcgcggt gagcgaatgc cgccgggggc agcgggttcg caagataggt
     7081 gcccggtgcc gcgggccgca ccatacttta ggacccctaa cccccggatt caccgaatct
     7141 tgtacgcctc atcacggcac attccgattc gcggcgcccg ttcgtgcgga ccgcccgtcg
     7201 atcgtgcgga tgcaccgtct acttcgcgta cgaaacggac cggacctcgc ggatcacggt
     7261 gaccttgatc tgcccggggt actgcatctc ggcctggatc cttttcgaga tgtcgacggc
     7321 gagctgctcg gccagggcat ccgatatccg gtcgtgattg acgaggaccc ggatctcgcg
     7381 gcccgcctgg atggcgtaca cccggtcgac gcccgggaac gaggcggcca gggcctccag
     7441 gttctcgagc cttcggatgt acgactccag cgcctcgcgc cgggcgcccg gccgagagcc
     7501 cgagatggcg tccgcggcct gcaccagggg cgaaaggagg gatgtcatgg ggatttcgtc
     7561 gtggtgcgcg cccacggcgt tgcagacctc ctcgtgctcc ttgaaccggc ggcacagctc
     7621 catgcccacg agcgcgtggg gctgctcgat gtcctcttcc gcgaccttcc cgatgtcgtg
     7681 cagcagcccg gcgcgccggg cgagcccggc atccaggtcg agctcggccg ccatgcgcga
     7741 cgcgaggcca gccacctcga tcgagtgggc cagcaggttc tgcccgtagc tcgaccggaa
     7801 ccgcatccgg ccaaccatgc ggacgagctc cggatgcacg cggctcagat tcagttcgat
     7861 gaccgtctgc tctccgatct ccaggatctc gtcctcgacc tccgtgcgcg tccggtccac
     7921 tatctcctcg atccgggccg gatggatccg gccgtcctgg atcaggcggt tcagggagat
     7981 gcgggcgatc tcgcgacgca cggggttgaa gccggaaaga atcactgcct cgggcgtgtc
     8041 gtcgacgatc acctcgatgc cggtcgccgc ctcgaaggcc cggatgttgc gcccctcgcg
     8101 gccgatgatg cggcccttca tgtcgtcgga cgcgaggttc acgaccgaga cggtgttctc
     8161 gatcgtgtgg ctcgccgccg tgcgctggat ggccgacagg accaccttgc gcgcctcccg
     8221 gttcgcctgg agcttcgcct cgtcccggac ctccttgacg agtgcggcgg cgtcgagacg
     8281 ggcctcgtcc gtcatctggt cgatgagcag gcgacgcgcc tcctcgcggg gcaggccgga
     8341 gacctgctcc aggcggccca cgtactcgcc gatggtctcg tcgagacgcc tggtacggtc
     8401 ctcgagcgct gccagctggc gatcgatctc cgcccgtgcg gcgtcgacac cctccgagcg
     8461 ctcccgcacc tgtccgaaca gcacttccgc gcggcgcaac acggtggccg tcgccgcctt
     8521 ctgctcctcg gcctcgacgc ggagcaggtc gattacgtcg gaggcctcct ggagaaggct
     8581 ctcctgctcg ccgatccggt gggcaatccg gctcagcctt tcgttacgtt tctcaagctt
     8641 ctggcgcgcg cgtcgccagt tgcgtcgggt ctcctcggct tcctcttgga gttcctgttc
     8701 ccgtcgtcgt aggtcttcct cggctttggc aatccgctgc tcgtgcagcg cttctatttc
     8761 cttctcggcg tcggcgagaa ggtggtgagc cttctcacgt gcctcggcca gacggttcag
     8821 gccgaagcgc ttcagtatga atctgtccag gattatgccg gtcccgagcg caactgctgc
     8881 gaccagcgcc accaggaagc ccacggtgag tgggtccatg gcacgtgttc ggttcgctgc
     8941 agggtgtggg cgcgggagtc ccacagaccc ggagagccct ccgcggatcc tggatgccgg
     9001 agcgccgcgc gccccggcgt ccgtccggcc ggaaggccgc gcaccgggtt cgcctgccgg
     9061 attatacgaa agaccccggc aggttccgat gccgtcggac gtcctgccgg ggtttccgca
     9121 gaatgggagc accggcccgc cgcgatgata tgaagaacct cctcatatca tggttgggta
     9181 ccgctcgagc ccttccgcct tccccggcgc ggcccctggg ggccgacaac gcccgaaggc
     9241 gcctggggcc tgatgtccgg actcaaagga tggtacccgc gagcgtagtc gttaggttct
     9301 tacaatgctc tcgaggcggg acagtgccga cgacaggata cggcgggcgg ccgagggcag
     9361 tcaagccgat ccggcgcgcc caggcggctc aggccttcga aaggagggcc gtcgccagct
     9421 cgcgttcgag ctcgtcgagg gcgacggcga tcgagtcccc gcggtcctcg cttcccgagc
     9481 gggccatgaa cagctcctcg gcgattgcca tcgccgagat caccgcggcc gtcgtgtcgg
     9541 actggtcggg atgcgcgtcc ttgaaggccc ggagcttcgc gtcgacgtag tgggcgagct
     9601 cgcgcgtgag gacctcgttc tcctcgcgaa cccggagcga gtaatcgcgg ccgccgatgt
     9661 tgatgcggat cgacttctgc atcacggggc ggggctcccc gagccgtctc ccgactcggt
     9721 cacggagcgg ccggcgaggt gcaggtcgat cgactcgatg aagcgctcga ccttcgagcg
     9781 cagcgcggcg gcgtcctcgg tgaagacgac cggcgtgccc tcgacctcgc cgagaccgcg
     9841 ggtctgaagc tcttcgacct ggcggtgcag gcggacgttc tcctcgcgca gccgcctgag
     9901 ctcgcgcgcg gtgaggctga tgcggtcgcg caggcgctcg agcgcccgct ccgtcttcat
     9961 ggacagcggg cgacggcctg tccgggaggc gtcgatggag tccggatcgg aggcatccga
    10021 gaacatgtcg ccctgggatg ggcctccagc ttcgggcatg tcacgctcgg gcgtctcgga
    10081 catggcacgg cggggatcgg gtcgcggaag ctgaatatac gtccggcggc gtccggcacc
    10141 atccccggcc gtcaggtacg caggacggct ccgtaccgct ccccgagcac ccggacgatc
    10201 cgctccacga cctcgtccac ctccgcgtcg cggagcgtcc ggtcggaggc gaagcgcatg
    10261 gagaaggcga cgctcttccg gccgggcccc aggcgctcgt cgcggtacag gtcgaagacg
    10321 cccgtccgcc ggagcaaggg tgccccggcc tcgcggatcg cgtcgagcag cgggccgacg
    10381 gcgacgcccg cgtcgacgac caccgcgatg tcgcggtcca cgacgggaaa tcgcgtgacg
    10441 tcccggtagc gcgtccatcc ccgcccggcg aggccgtcgg cgacggctcc gaggtcgaac
    10501 tcggcgaaga acacctcgcc cctcaggtcg aagcggtcca gcacgtcgcg gcccacggcg
    10561 gcgagggagc caatcggccg gtcgccgagc cggatgacca ggtggtagcg cgtgagcggg
    10621 gtcggctcgc gcacgggatc gagcctgtac ccggacacgc cgagcccctc gaagagcgcc
    10681 tccacgtggc cgcgcaggtc gaagacgtcg gccgaacgtc ccttggtgtc ccagcccggc
    10741 tccgagatcc ggcccgtgac ggcgacgagc agggacgtgc gctcccggta cccggcgacc
    10801 ggcgcgccgg gcgcgtcgaa ccggtccatg acgaccccga tctcgaacat cctcagtccg
    10861 gcctggccgt gattcaggtt gtgcgcgagc acctgcagca tcccggggag gaggctcggg
    10921 cgaagcgccg ccatctcggt cgagatgggg ttcagcgtct ggacggcctc gccgaccgcg
    10981 actccgcccc gcagcaccgg gtgcgcgaag agcgtggcca cggccggggg cagcatcgag
    11041 ttcgtacaga tctcgcggaa gcccgcgccc gagaggatct gcgccgcccg gtcccgaacg
    11101 ggctgctcgg cggggctggt aggcagcacg ttggggaggc ggctgtgcgt gggctccggg
    11161 atgcggtcca agccatggag gcgcgcgatc tcctcgatga cgtcgatctc gcgctccacg
    11221 tcgggtcgcc acgtggggac ggtcaccgac aggccgtcgt ccctgcgccg cggctcgaag
    11281 ccgatggccc gcagcaggcg ctcggcgtcc gccgccggca cgtccacgcc gagtacggcc
    11341 gcgattctcg cggagcgcac gtgcagggtg cggcgctcgg ccggcaccgg gtgtgcatcg
    11401 accatgccgt ccaccagggt gccgcccgcg atctggacca tgagggccgc cgcccgcgcg
    11461 gccgcccaca cctggccggc cgggtccacc ccgcgctcga accggtacga ggcgtccgac
    11521 ggcagctgga gggccttcgc cgtgcggcgg atgtcggacg gatcgaagta cgcgctctcg
    11581 atcaggacgt cggtcgtcgc ctgggtcacc tcggaattct cgccgcccat gacgccggcg
    11641 acggccactt cgcgctcgcc gtccgcgatc atgagcgttc ccttcgggag cgtgcggacc
    11701 ttgccgtcga gcgtcgtgaa cggcccctcg gccgccgtcc ggcggacgac gatgcggtgg
    11761 ccggcgagct ggtcgaagtc gaacgcgtgc agcggctgcc cgcactcgtg catcacgaag
    11821 ttggtgatgt cgaccacgtt gttgcggggg cgcaggccga tcgccgccag gcgctgcctg
    11881 agccacgccg gagactcggc gatcgtgacg ccgcgcacca ggagccccac gtaccggtta
    11941 cagccgtccg gcgcctcgat gtccaccgag aatgcgaggg cggcctcgcc tccctctcgc
    12001 ggaagtgcga cgtcggggcg gcggaggggc cggtccgcga gggccgccac gtcccgggcg
    12061 acgcccaggt ggctcgtcgc gtccggccgg ttcggggtca cggccacgtc gagcaccgta
    12121 tccgtggccg ggtagccaga gcgctcgagg tacgccgcga acgactcgcc gggacgcccg
    12181 tgctcctcga gcaccatgat gccggagtgg tcgtcggaaa gtccgagctc gtcctccgcg
    12241 cagatcatcc cgtgcgacac ctcgccgcgg attctcgcgc tgcggagcgt gacgggcacg
    12301 cggacgcccg ggtcgtcgcg gtccgggaga tgcagcgtcg tgccgaccgt cgcgacgggc
    12361 accacctggc ccgcggcgac gttcggcgcc ccgcagacga tctggacggc ctcgccgttg
    12421 cccgtgtcga cgtcgcagag ggtgaggcgg tcggcctcgg ggtgcgcgcg gacgcccagg
    12481 acccggccca cgaccacgcc gtcgagggtc gggccgacgg cttccaccga ttcgacctcc
    12541 agtccgcaga gcgtgagccg gtcggcgagc tcttcggccg accagtcgtg gtcaacgtac
    12601 gtgcgaagcc agcgggtact gattttcatg gctcgagctg ggagaggggc ggctcaggtg
    12661 aactgttcca ggaaccgcac gtcgttctcg tacaggatgc ggatgtcgga gatgtcgtag
    12721 cgcagcatgg cgatgcgctc cacgcccatc ccgaacgcat agccggtgta ccgctccggg
    12781 tccacgtcga cggcctccag gaccgccggg tcgaccattc cgcagcccag gatctcgagc
    12841 caccggcctc cctcggggcg gctctcgtcg cgccaccaga tgtcgacttc ggcgctgggc
    12901 tcggtgaacg ggaagaagct cgggcggaag cgcatgcgca cgtcgtcgcc gaagagggcg
    12961 cgcgcgaacg tgtgcaggac ctccttcagg tccgccatcg acacgccctc gtccacgtac
    13021 agtccctcga cctggtggaa caggcagtag gacttgtagg agagggcctc gttgcggtac
    13081 acgcggcccg ggac
//
LOCUS       DBNHQZ010000286         4131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1576468, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000286 DBNHQZ010000000
VERSION     DBNHQZ010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1576468"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16YU_07725"
     CDS             complement(<1..644)
                     /locus_tag="AC16YU_07725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR6065151.1"
                     /translation="MSASSAELLSARLASREAEIGVVGLGYVGLPLAVEFAVRGFRVH
                     GIDLSADRVRRIAEGRNYIDDVADADFGEATRSGRLTAASDFDAAARCDAVFICVPTP
                     VTLHKDPDVSYIRSAALALAPHLHAGQAVILKSTTYPDTTEGIVQPILEEAAAVKGLE
                     LGRDWFLAFSPERIDPGNRQFTTANTPVVVGGVTDACTEVAAGIMGQVVSTVHRV"
     gene            complement(853..1518)
                     /locus_tag="AC16YU_07730"
     CDS             complement(853..1518)
                     /locus_tag="AC16YU_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6065152.1"
                     /translation="MALRKTEEADIRTRYPLYVEIGMVLTLALLIVAFRLDLRSDGEF
                     DVTMQEQEVVQMEEILQTQQVEKPPPPPRPPVPVEVPNDEILEDEDLDLDASLDLDEP
                     LADLPPPPPPPPSDEPEPEPEIFVIVEQMPELIGGLSELQKKISYPEIAKKAGVEGRV
                     IVQFVVNEEGNVTDPVVVRGIGAGCDEEAIRAVRTAKFTPGKQRGKPVRVKMSLPITF
                     KLK"
     gene            complement(1653..2063)
                     /locus_tag="AC16YU_07735"
     CDS             complement(1653..2063)
                     /locus_tag="AC16YU_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6065153.1"
                     /translation="MSGSDSTGRRRPWPAYAWSALILVAMLIPGAYVPGSMWSLDKLA
                     HFVLFAGFAWLWLRAAPGAWLRVGIVGLLYGVLTEVGQSVFPGERTGDPMDVLANVLG
                     LAAGILLPLAAARRKRRRLRDGRPHTDRSNRGGG"
     gene            complement(2047..3615)
                     /locus_tag="AC16YU_07740"
     CDS             complement(2047..3615)
                     /locus_tag="AC16YU_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR6065154.1"
                     /translation="MAPTDFAAFALTAAAMREADRRTIEDLGIPGHTLMETAARGAVA
                     AVEARQGGLAGRRVAILCGRGNNGGDGLAMARVLHARGAEVDVYLFASADALAGDAAL
                     NYRVLEAVARAESDRLRMHGHEELPEAGAFDLVVDALLGTGQASPPRPPIDAGVAWLN
                     ARRARVVSVDLPTGVHTDTGAVLGEAVRAELTVTMGAPKTGLLLGEGRSHAGTVVPVE
                     IGIPAHVIREVAQAEGCARITTDAWVGSLLRGVERGGTKFSSGPTLVAGGSRRYAGAP
                     VMAATAAARAGSGYVVCAAPEAARAAIASHLVEIPFVGLPSDPEGFVDGESAASVLDE
                     AWPRVRAFLVGPGIGRTDGARRFVRAVLAGRDGTVVVDADGLNAIDAEFVAAHSGGRW
                     ILTPHEAEFERLGGPVGDRSPVVRAAEAARAWGTAVVLKGLPSVVAAPDGRVAIAGTG
                     NPALATAGTGDVLAGLCAGLAARGLEPFEAAVAGLHLGGAAADRFAARSGRSSMMATD
                     LIRQIGPVLHERFR"
     gene            complement(3627..>4131)
                     /locus_tag="AC16YU_07745"
     CDS             complement(3627..>4131)
                     /locus_tag="AC16YU_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6065155.1"
                     /translation="IAFSLHPDAGAAFRVSPDPAPGLPFDISVRPLAPGESDTTWTAR
                     FALAGPARLGELSGVVLGGAPVVVELHPATARSEPVRRALADTDGRFRFDRLPGGRRY
                     RIRAFVDEDGDGAWSPGSPAPYASPEPLAWSDTAPPVRARWETEIPDTLRIGAPAVRV
                     PPGDDGP"
BASE COUNT          581 a         1461 c         1550 g          539 t
ORIGIN      
        1 acccggtgca cggtcgacac gacctgcccc atgattccgg ccgcgacctc ggtgcaggcg
       61 tccgtgaccc cgccgaccac gaccggggtg ttcgccgtcg tgaactggcg gttgcccggg
      121 tcgatgcgtt cggggctgaa tgcgaggaac cagtcccgcc ccagttcgag tcccttcacg
      181 gctgcggcct cctccaggat cggctggacg attccctctg tggtgtccgg gtacgtcgta
      241 ctcttcagga tcacggcctg gccggcgtgc aggtggggcg cgagcgccag cgccgcactc
      301 cggatgtacg acacgtcggg atccttgtgc agggtgaccg gcgtggggac gcagatgaag
      361 acggcgtcgc agcgggcggc ggcgtcgaag tccgaggccg cggtgagcct gccggatcgg
      421 gtcgcttccc cgaaatcggc atcggccacg tcgtcgatgt agttccggcc ctcggcgatc
      481 cggcggacgc ggtcggccga caggtcgatg ccgtgcacgc ggaagcccct gacggcgaat
      541 tccacggcga ggggaaggcc cacgtagccc aggccgacga ctccgatctc ggcctcccgg
      601 gatgcgagtc gggccgagag cagttcggcg gaagatgcgg acatcggacg ggaccggaac
      661 gattggggac agggaacgga ggccggcggc tccggccgtc tgcaacgccg cccggaagcc
      721 ttcgatccgt gcagttccgg aggagaaccg cgtggctccg gaggaggcct gcgcgggacg
      781 cgggcgggcc atgaatggaa aaaggtcccg ccggtgctcc gacgggacct ctttccgcag
      841 atccggggcc gactacttca gcttgaacgt gatcggcagt gacatcttga cgcgcacggg
      901 cttgccacgc tgcttgcccg gcgtgaattt ggcggtccgg accgcgcgga tggcttcctc
      961 gtcgcacccg gcgccgatgc cgcggacgac gaccgggtcc gtgacgttac cctcttcgtt
     1021 gaccacgaac tggacgatca cgcgcccctc gacgccggcc ttcttggcga tctcgggata
     1081 gctgatcttc ttctggagct cggagaggcc gccgatcagc tcgggcatct gctccacgat
     1141 cacgaagatc tcgggctcgg gctcgggctc gtcggagggc ggcggaggcg gaggcggagg
     1201 caggtcggcg agcggctcgt cgagatccag ggaggcgtcg agatccaggt cctcgtcctc
     1261 gaggatctcg tcattgggca cctcgacggg gaccggcggg cgcggcggcg gcggcggctt
     1321 ctccacctgc tgcgtctgca ggatttcctc catctggacg acctcctgct cctgcatcgt
     1381 cacatcgaac tcgccgtccg aacgcaggtc caggcggaag gcgacgatga gcagggcgag
     1441 cgtgaggacc attccgatct ccacgtacag gggataccgc gtgcggatat cggcctcttc
     1501 ggtttttcgc agggccattt cttctggaac cgggagagtg gtgggagagg gcacgcaagg
     1561 tacggccaag ggccgttccg gcacagcgcg cgcgaatttc atgttaacaa cggtccgcgc
     1621 ggcgcgcggg ttccggcccg ttccggtgca tttcagccgc ccccgcgatt cgagcggtcc
     1681 gtgtgggggc ggccgtctcg gagtctccgg cgcttccggc gggccgcggc gagcggcagc
     1741 aggatgcccg ccgcgaggcc gagcacgttc gcgagcacgt ccatcgggtc gccggtgcgc
     1801 tcacccggga atacggactg gcccacttcc gtgagcacgc cgtacaggag gcccacgatg
     1861 ccgacccgga gccaggcgcc gggcgcggcc cggagccaga gccatgcgaa cccggcgaag
     1921 agcacgaagt gggcgagctt gtccaggctc cacatcgagc ccggcacgta cgccccgggg
     1981 atgagcatgg ccaccaggat gagcgcggac caggcatagg ccggccaggg gcggcgcctg
     2041 ccggtgctat cggaaccgct catggagcac gggtccgatc tggcggatga ggtcggtggc
     2101 catcatgctg ctcctgcccg aacgcgcggc gaaccggtcg gcggccgctc ctccgaggtg
     2161 caggccggcg acggccgcct cgaagggctc gaggccgcgc gccgcgaggc cggcgcagag
     2221 gccggcgagc acgtcgcccg ttccggccgt cgcgagggct ggattcccgg tgccggcgat
     2281 cgcgacgcgg ccgtcgggcg cggcgacgac cgagggcagg cccttgagca cgacggccgt
     2341 cccccacgcg cgggccgcct cggccgcgcg gaccaccggc gagcggtcgc cgaccggacc
     2401 gcccagccgc tcgaactcgg cctcgtgcgg cgtgaggatc cagcggccgc ccgagtgggc
     2461 ggcgacgaat tccgcgtcga tcgcgttcag cccgtcggca tccacgacga ccgtgccgtc
     2521 gcggcccgcg aggaccgccc gcacgaagcg gcgggcgccg tccgtccgcc cgatgccggg
     2581 gccgaccagg aaagcgcgga cccgcggcca ggcctcgtcc agtacggacg ccgccgactc
     2641 gccgtccacg aagccctcgg gatcggaggg caggccgacg aacgggatct ccacgaggtg
     2701 cgaggcgatc gccgcccgcg cggcctcggg ggcggcgcag accacgtacc cgctgccggc
     2761 ccgtgcggcg gcggtcgcgg ccatgaccgg ggccccggcg taacggcggg atcctcccgc
     2821 gacgagcgtc ggcccgctcg agaacttcgt cccgccccgc tcgacgccgc ggagcagcga
     2881 tccgacccag gcgtcggtgg tgatgcgcgc gcagccttcg gcctgcgcca cctcccggat
     2941 gacgtgggcg ggaatgccga tctccaccgg cacgaccgtt ccggcgtgcg agcgtccctc
     3001 gcccaggagc agtcccgtct tgggcgcgcc catggtcacg gtgagttccg cccggacggc
     3061 ctcgcccagc acggcgccgg tgtccgtgtg gacgcccgtc ggcagatcca cgctcacgac
     3121 gcgggcgcgc cgggcgttca gccacgcgac gcccgcgtcg atcgggggcc ggggcgggct
     3181 cgcctgcccc gtgcccagga gggcatcgac gaccaggtcg aaggcacccg cttcgggcag
     3241 ctcctcgtgg ccgtgcatcc gcaggcggtc ggactccgcg cgggcaacgg cctcgagcac
     3301 ccggtagttg agagccgcgt ctcccgcgag ggcgtcggcg gacgcgaaga ggtacacgtc
     3361 gacctcggcg ccccgtgcgt gcaggacgcg cgccatcgcg agcccgtccc cgccgttgtt
     3421 gccgcgcccg cagaggatgg cgacgcggcg ccccgccaag ccgccctggc gggcctcgac
     3481 cgccgcgacc gcaccgcggg cggcggtctc catcagcgtg tggcccggga tgcccaggtc
     3541 ctcgatcgtc cgccggtcgg cctcccgcat ggcggcggcc gtgagcgcga aggcagcgaa
     3601 gtccgtggga gccatggggt cgttcgtcag gggccgtcgt cgccgggtgg tacgcgaacg
     3661 gccggggctc cgatccggag ggtgtcgggg atctcggtct cccagcgcgc gcggacgggc
     3721 ggggccgtgt ccgaccaggc caggggctcc ggactcgcgt acggagccgg actgccgggc
     3781 gaccaggcgc cgtcgccgtc ctcgtcgacg aacgcccgga tccggtaccg ccggccgccg
     3841 ggaagccggt cgaagcggaa gcgcccgtca gtatctgcga gcgcccggcg cacgggctcg
     3901 gaccgggccg tcgcgggatg cagctcgacc acgacaggcg cgccaccgag gacgacgccc
     3961 gagagctcgc ccaggcgcgc cggccccgcg agcgcgaacc gggccgtcca cgtggtgtcg
     4021 gactcgccgg gcgccagggg gcggaccgag atgtcgaacg gaagtcccgg ggccggatcg
     4081 ggcgagacgc ggaatgccgc gccggcgtcg gggtgaagcg agaaggcgat c
//
LOCUS       DBNHQZ010000287        12293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1169767, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000287 DBNHQZ010000000
VERSION     DBNHQZ010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1169767"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="AC16YU_07750"
     CDS             <1..1383
                     /locus_tag="AC16YU_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIR6065156.1"
                     /translation="LLILYLTDEIVNGGRGMTVLHAGALYGLYTSMVYVTNLPGGWIA
                     DKFLGARNAVFYGGILIAVGNLCLAAPYDATFYVGLGFIALGTGLLKPNVSTMVGSLY
                     EKGDVRRDSGFTIFYMGINIGAGIAPFIIGYMGQNIAWRLGFAGVAVGMVLGLIQYRM
                     GAKYLGDAGVHRPAGTPEGRARERRSLYYGLLAVTAVIVIPILLNASGIYTITIDWVR
                     NLVGVVLIALPLVYFGFLFARGGFTKEERGRIIAIIIFYIAAALFWGSFEQAGSTLNL
                     FADRFTDNRILGWEFPSTWWQSVNAIYIVLLAPVFAWLWIKLASIKREPSTPVKFALG
                     LLFVGLGFLLLVPAAKQLIASGEGAKVGVQWLLGVYLIHTIGELCLSPVGLSAMTKLA
                     PERIVGQMMGIWFLGAATGNFIGGNVGGLFETFPLDKIFMAVFATSAVAALLMFVLVP
                     WMRRRMGGIH"
     gene            complement(1523..2767)
                     /locus_tag="AC16YU_07755"
     CDS             complement(1523..2767)
                     /locus_tag="AC16YU_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065157.1"
                     /translation="MIRILLQEFWNDLRTQKTRAFLTMFAIAWGTISVVLLLAFGEGL
                     GKTMRDGMLNAGDRIFMVYGGQTSKAFEGLGEGRRIPLVRADLDLLMRSLPGIDMGSV
                     SYGRWGVALRVGDNRTTTYMEGVDPSFEDLRRMFPAAGGRFLSDRDNEMKRRVVFLGD
                     EIAARLFGEDEPVGRDVMIDGLPFTVVGVMQSKLQTAMNNGPDAERAIIPSSTFETIY
                     GHRYINHMLIRPRSSAVAPTVKAGIFEVLGRKYKFHADDRRALGLWDMLEAIETGAKV
                     FFGIQVFLGLVGGLTLLLAGVGVANIMYVTVKERTPEFGVKLAMGARKRHIKAQVVFE
                     ALLIALTGGAIGMLFSVAVVTAVRSIPNKEGAMEFLANPEISVPIALVTAIILTAIGL
                     LAGYFPARKAARLDPVESLRYE"
     gene            complement(2777..4006)
                     /locus_tag="AC16YU_07760"
     CDS             complement(2777..4006)
                     /locus_tag="AC16YU_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065158.1"
                     /translation="MAILDSIGQFVQDLRTQRLRTTLTILGIAWGTIAVVVLLAFGVG
                     LEKQSRKNMHGMGNGVVVVFPGRTTIPFEGFPDGRRIRLVEADVATLRAQIPDIGQIS
                     AEYTRRVPMRRGRSTTTPTVTGIYPEYGDMRNIIPETGGRFLNPTDEAQRRRVVVLGD
                     EIDRLLFGEEDAIGRQVMIGDTPFTVVGVMQKKTQNSSYNTRDQDRVFIPSSTFKSIF
                     GDRYINNLIYRPVDPDRSPAIKASMFQILGRKHRFDSSDNDALGVWDTNQMDKMMKYF
                     FIGFNVFMGIIGSFTLTVGGIGVANIMYVVVRERTREIGIKRSVGATRRSILAQFFAE
                     TLTIVGTGALIGFVAAVGLVELLGMLPVEEYVGTPRISGMVAATTVLLLIGIAILAGY
                     FPARRASRLDPVDCLRG"
     gene            complement(4025..5149)
                     /locus_tag="AC16YU_07765"
     CDS             complement(4025..5149)
                     /locus_tag="AC16YU_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6065159.1"
                     /translation="MKRLLFIAGALVVAAAVTAFVIRRNGHSDEEARPHVEVTRGSIV
                     DRALAVGTIEPRVEIEVKSQVSGVVKRQFAEAGEFVRAGDPLLELQPNPTPRELMEAE
                     RQIEQRELELQNLKDEYDRTKGLYDKQLIAVHEYERVKRTYDEALLAVRTAKEQLTLL
                     REGKVNTRSGSVETVVRAPVSGFVLDKMVEIGDPVVPLTSYQEGTVLMTMADMDDLIF
                     RGTVDEIDVGRLKEGMTAAIKVGALPGAEVSGELARIWLKARKEENSTVFPVEVEIRS
                     AVERDPDDTEAEPVPVVLRAGYSANTEIVIESREDVLLVPERVVHFNGDSTLVEILLD
                     DNTTEERLIRTGLSDAINIEIVSGLDEGMKIAEKPPKVIE"
     gene            complement(5236..6318)
                     /locus_tag="AC16YU_07770"
     CDS             complement(5236..6318)
                     /locus_tag="AC16YU_07770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6065160.1"
                     /translation="MATERLAELLETTYQVVRFNTEPVDEGFVAAETRWRTGRVLHYL
                     RQRFILTRALSQYPAAPVVWTSISPTLLGHVRDVVSVLPSFGRKRPVIAVSHRATLGD
                     RFEDGRTRATMGRIERRSSVFVFLTNAISESVSTWIPDGKRAVIPNTIAEALLATTGD
                     LEAKRARRHADERLELLFVSNMLPEKGWMDILRAVPLVRREGHRVRIRFAGRWVRAED
                     EERFAQVVAENALDDCVEHLGGVTEAGEIRRLYQSSDALLLPTYHPTEGMPLAILEAF
                     AWSMPVVGTRQGGLADLIEDGENGIVVPPRDPAALARAISALTDRTRLLRLSDGARRT
                     FVARFDPDAVQTAWIQLIDSVCPGRA"
     gene            6524..7699
                     /locus_tag="AC16YU_07775"
     CDS             6524..7699
                     /locus_tag="AC16YU_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065161.1"
                     /translation="MTQQDFEVLRELAPWINSLGRVGALSPLREWEGRLFPRAVRIPR
                     WKWDLNRPSRRRFDVLLAANVFMYSPDPARWFRNVLASCRWFLLVDLVRRRRSEVDEF
                     GTDRDGMRFAVGSERPRIDGFFDLGSFGDRLLAWRTYYGGANPLDDAPLHVVALIRGD
                     LGEPILRIDDYPTGVRPILDDMSPLHDILERVEAYELSYHLGIVPALVQDEMASFLRS
                     LQHMVPVMHGFDHCYPKYSRLLTEVGDPYNEKTVGVFNEFGGKSYAEIVALLSDGRAR
                     LAEDVGMEPRWYIPPCNWGDRRTGRALQEAGFDGYLSEKRIPGCSLPWHASDFYGRSS
                     GYDLSREPDVVTLHLTWEWDLLRSRSQSRLDELLTSLVSRRLQLDGQIRPLRDRIDG"
     gene            complement(7848..8600)
                     /locus_tag="AC16YU_07780"
     CDS             complement(7848..8600)
                     /locus_tag="AC16YU_07780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6065162.1"
                     /translation="MDRWHPLDPYVVPELRKLAPRVESVALPDAFQRVSRGRTADEIR
                     REWGVEPHRRIGLFFGSIGARKGIHAVLRSLSLLPDRLQDRFCLVIAGPHWESESAEI
                     ARSIEAARSLAGPQIVVRNQYVGDDDIQDVIRASDVVLVAYQRHIGSSGVLIRAAAEG
                     VPVVASDWGLVGHYVRDRKLGVVADAEEPSAIAEALARVLNRDLPDRIFDPGEARRFA
                     AEHTLEEFGRVLFADGPSGSRPPGHPDCEPFR"
     gene            complement(8560..9111)
                     /locus_tag="AC16YU_07785"
     CDS             complement(8560..9111)
                     /locus_tag="AC16YU_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065163.1"
                     /translation="MPPYRILFDLYASGHHPSYLLQTARVWLDRGEKGRLGVAAPGAM
                     FDAHPEITGWLADHERDGIRMVPLPLLENPARWLGLMGSLVRSDVEHGRILRRLVESE
                     KPDRVILMFADHAQLSLGLGLRFDYPLRFSGIYFGPSFHYRSLFGSPVSRLSAVRKRT
                     ILRLALSNPPRGSMAPSRSVRRA"
     gene            9369..10268
                     /locus_tag="AC16YU_07790"
     CDS             9369..10268
                     /locus_tag="AC16YU_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065164.1"
                     /translation="MAQERPPVFLEHRSNVHSQFGEDGIVEWILEQLDLSNGFFVEFG
                     AWDGLYLSNCRRLYERGWSGCFIEGDARRFRDLERTYAGSDRAVLVQAFVSPEGDTRL
                     DALLRRSEVERVDLLSIDIDSDDLAIWQSVHEYRAKCVVIEYNPTIPFDTRFQNPRGR
                     NVGNSALSIVEHAHEIGYDLVAGTYTNLVFVDSSARPESIPTVTLQQIKDYAEGAFRY
                     FFAYDGTLMRESFNTIRETELFRIPWSGAVGIQPLPRILRRFDRLNGVKKVLNGLVAI
                     VLRPVGSVRMLMSIRGDRREPMS"
     gene            10330..11826
                     /locus_tag="AC16YU_07795"
     CDS             10330..11826
                     /locus_tag="AC16YU_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6065165.1"
                     /translation="MNVRRLLLNAGSASFQVILSAIVLFVVYGYIVRELGIDLFGVWA
                     TVAASTSVAAIGGLGLAGGVVKFVAKYRALGDLRRVSIVLSTSMVAAAIALGIMAALG
                     LLLLPSVLPWLMDRPDELEVGIDLVPYVVVSFWLTSVSAIGVSGLDGFNRIDIRAAVY
                     VGAMIVYAMLVFILLPRHGIIGLVEAQIVQGVLLLVVPWIVLGVLTRHRGMLPLRWSN
                     DVFREVVGYGVSFQVIGIAQILFEPTTKALLTKFGGAGATGIFEMANRLAFQARSVID
                     ASHRSIVPSLAELHELDAPVLPAIYTRSAKLLVMVSACLLPTLAVLMPVVSVAWLGTL
                     DITFVVVSVLLSTAWMLNLLATPAYYANLGTGALRWNVIGTVVTAIMNISLGFTLGRW
                     WGPGGVVAVFVLSLLVGNALMVWDYHRRHPGFAEAFVEPGDMPLVVGSIIIALAGALL
                     GPMALAATTPIAYLIAAIVLLGASLLPLAMRHSGYRLLRGLTRALDRR"
     gene            complement(11801..>12293)
                     /locus_tag="AC16YU_07800"
     CDS             complement(11801..>12293)
                     /locus_tag="AC16YU_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6065166.1"
                     /translation="TATTEDMSLVNRWRESAAVLEKVVNNPVLGHGMGVPFRFYDVQF
                     IPPKTLVKSFLHNGYLALWYKFGLWGLGMMLYFWGTMITRGIAAYRSSGRFVYRLSAL
                     TSAAFLVSLTLAAITSNPFNIDDPVFMFAILSGLAAGAFDRTRAADRPGTTPEASDGR
                     GPG"
BASE COUNT         2036 a         3997 c         3964 g         2296 t
ORIGIN      
        1 ctcctcatcc tgtacctgac cgacgagatc gtgaacgggg gccgcggcat gaccgtgctg
       61 cacgccggcg cgctctacgg cctgtacacg tcgatggtct acgtgaccaa cctccccggc
      121 ggatggatcg ccgacaagtt cctgggcgcg cgcaacgcgg tgttctacgg cggcatcctg
      181 atcgccgtcg gcaacctgtg cctcgcggcg ccctacgacg cgacgttcta cgtgggcctc
      241 gggttcatcg cgctcgggac cgggctcctg aagccgaacg tttcgaccat ggtcggctcg
      301 ctctatgaaa agggcgacgt ccggcgcgac tccgggttca ccatcttcta catgggaatc
      361 aacatcgggg ccggcatcgc gcctttcatc atcgggtaca tgggccagaa catcgcctgg
      421 cgcctgggct tcgcgggagt tgccgtgggc atggtcctgg gactcatcca gtaccggatg
      481 ggcgcgaagt acctcggcga cgcgggcgtg cacaggccgg ccggcacccc ggagggccgg
      541 gccagggagc gccgctcgct ctactacggc ctgctcgccg tgaccgcggt catcgtcatc
      601 ccgatcctgc tcaacgcgtc cgggatctac acgatcacga tcgactgggt gcgcaacctg
      661 gtcggtgtcg tgctcatcgc gctgccgctg gtatacttcg gattcctgtt cgcgaggggg
      721 ggcttcacga aggaagagcg tggccggatc atcgccatca tcatcttcta catcgcggcc
      781 gccctcttct ggggttcgtt cgagcaggcc ggctccacgc tgaacctgtt cgccgaccgc
      841 ttcaccgaca accggatcct gggctgggaa ttcccgtcca cctggtggca gtcggtcaac
      901 gcgatctaca tcgtgctcct ggcaccggtg ttcgcctggc tctggatcaa gcttgccagc
      961 atcaagcgcg agccctcgac gcccgtcaag ttcgcgctcg gactgctgtt cgtgggcctc
     1021 ggctttctgc tcctcgtgcc cgcggccaag cagctcatcg cgagcggcga gggcgcgaag
     1081 gtcggcgtgc agtggctcct gggcgtctac ctgatccaca cgatcggcga gctctgcctc
     1141 tcgccggtcg gtctctccgc aatgaccaag ctcgcccccg agcgcatcgt cggccagatg
     1201 atgggcatct ggttccttgg cgccgccacc ggcaacttca tcggcggcaa cgtgggcggc
     1261 ctgttcgaga ccttcccgct cgacaagatc ttcatggccg tcttcgcgac cagcgcggtc
     1321 gcggcgctgc tcatgttcgt gctggtgccg tggatgcgtc gccgcatggg gggaattcac
     1381 taggccgccc cggggatctc ggacgccctc gtctggcggt cacgctggat gctcgaagcc
     1441 acctcgtccg ctcgtcgcga tggcttggcg tcccgaatga gagtacggtc aggcagaccc
     1501 gcccgagcga gcctgccccg tcctactcgt accgcagcga ttccaccggg tcgagccggg
     1561 ccgccttccg cgccgggaag tacccggcga gcaggccgat cgccgtcagg atgatcgccg
     1621 tgaccagggc gatcggcacc gagatctcgg ggttggccag gaattccatc gccccttcct
     1681 tgttcgggat gctccgaacg gcagtgacca cagccaccga gaagagcatg cctatggccc
     1741 cgcccgtcag ggcgatcagc agcgcctcga acacgacctg ggccttgatg tgccgcttgc
     1801 gggctcccat ggccagcttc accccgaact ccggggtgcg ctccttcacg gtcacgtaca
     1861 tgatgttggc cacgccgacg ccggccagga gcagggtcag gcccccgacc agccccagga
     1921 agacctggat cccaaagaac accttcgcgc cggtttcgat ggcctcgagc atgtcccaga
     1981 ggccgagcgc ccgccggtcg tccgcgtgga acttgtactt gcgccccagc acctcgaaga
     2041 tgcccgcctt caccgtggga gccacggccg agctcctcgg gcggatgagc atgtggttga
     2101 tgtaccggtg gccgtagatc gtctcgaagg tcgaggaggg gatgatggcc cgttcggcgt
     2161 ccggcccgtt gttcatggcg gtctgcagct tggactgcat gacgccgacg accgtgaagg
     2221 gcagtccgtc gatcatgacg tcacggccga cgggctcgtc ctcgccgaag agccgcgccg
     2281 cgatctcgtc gccgaggaac accacgcgcc gcttcatctc gttgtcgcga tcgctaagga
     2341 agcgcccgcc cgccgcgggg aacatgcgcc gcagatcttc gaaggacgga tccacgccct
     2401 ccatgtaggt tgtcgtgcgg ttgtcaccca cccgcagggc cacgccccag cgcccgtacg
     2461 acacgctgcc catgtcgatg cccggcagcg accgcatcaa gaggtccagg tcggcacgca
     2521 ccagcggaat gcggcgcccc tcgcccagac cctcgaacgc cttggacgtc tgcccgccgt
     2581 agaccatgaa gatccggtcg ccggcgttga gcatgccgtc gcgcatggtc ttgcccaggc
     2641 cctcgccgaa cgcgagcagg agcacgaccg agatcgtccc ccaggcgatg gcgaacatgg
     2701 tcaggaaggc ccgcgtcttc tgcgtgcgca ggtcgttcca gaattcctgc aggaggatgc
     2761 ggatcatggc gttggatcag ccgcggaggc agtccacggg gtcgagccgg gacgcccggc
     2821 gcgcgggaaa gtatccggcg aggatggcga tgccgatgag cagcaggacg gtggtcgcgg
     2881 caaccatgcc cgagatgcgc ggcgtgccca cgtactcctc cacgggcagc atgcccagta
     2941 gctccaccag gccgacggcg gctacgaatc cgatcagcgc gccggtgccc acgatggtca
     3001 gggtttccgc gaagaactgg gcgaggatgg agcgacgcgt agcgccgacc gaccgcttga
     3061 tgccgatctc gcgcgtgcgc tcgcgcacca ccacgtacat gatgttggcg actccgattc
     3121 cgccgaccgt cagcgtgaag ctcccgatga tgcccatgaa cacgttgaac ccgatgaaga
     3181 agtacttcat catcttgtcc atctggttcg tgtcccagac gccgagcgca tcgttgtccg
     3241 acgaatcgaa ccggtgcttc cgccccagga tctggaacat cgatgccttg atggcgggag
     3301 accggtcggg gtcgacgggc ctgtagatca ggttgttgat gtaccggtcc ccgaagatcg
     3361 acttgaacgt ggaagagggg atgaacaccc ggtcctggtc gcgcgtgttg taggacgagt
     3421 tctgggtctt cttctgcatc acgccgacga ccgtgaacgg cgtgtcgccg atcatgacct
     3481 gccggccgat cgcatcctcc tcaccgaaga gcaggcggtc gatctcgtcg ccgagcacca
     3541 ccacgcgccg gcgctgcgcc tcgtcggtcg gattcaggaa gcggccgccc gtttccggga
     3601 tgatgttgcg catgtcgccg tactccgggt agatcccggt cacggtcggg gtcgtggtgc
     3661 tgcgcccccg acgcatcggg acccggcgcg tgtactcggc gctgatctgc ccgatgtcgg
     3721 ggatctgcgc ccggagtgtg gccacgtccg cctccacgag gcggatccgg cgtccgtccg
     3781 ggaagccctc gaacggaatc gtcgtgcggc cgggaaaaac gacgaccacg ccgttcccca
     3841 tgccgtgcat gttcttgcgc gactgcttct ccaggcccac cccgaacgcg agcagcacga
     3901 cgacggcgat cgtgccccag gcgatgccca ggatggtcag cgtcgtgcgc agccgctgcg
     3961 tgcgcaggtc ctggacgaac tgcccgatgg agtccagaat ggccatggtg cccggtagtc
     4021 cgggttattc gatgaccttc ggcggcttct cggcgatctt catgccctcg tcgagaccgg
     4081 agacgatctc gatgttgatg gcatccgaga gaccggtcct gatcaggcgc tcctcggtgg
     4141 tgttgtcgtc gagcaggatc tcgacgagcg tcgagtcgcc gttgaagtgg accacgcgct
     4201 cggggacgag cagcacgtcc tcccggctct cgatgacgat ctcggtgttc gccgagtacc
     4261 cggcgcgcag gaccacgggc acgggctcgg cctcggtgtc gtccggatca cgctcgacgg
     4321 cgctccggat ctccacctcg acggggaaga ccgtcgaatt ctcttccttg cgcgccttga
     4381 gccagatgcg cgcgagctcg cccgagacct cggcgccggg cagcgcaccg accttgatcg
     4441 ccgcggtcat gccctccttc aggcgcccca cgtcgatctc gtcgaccgtg ccgcggaaga
     4501 tcaggtcatc catgtcggcc atcgtcatga gaacggtgcc ctcctggtag gaggtgagcg
     4561 gcacgacggg atcgccgatc tcgaccatct tgtccaggac gaagccgctg actggcgcgc
     4621 ggaccacggt ctcgacggag ccgctgcgcg tgttcacctt gccctccctg aggagcgtga
     4681 gctgctcctt cgcggtgcgg acggcgagaa gcgcctcgtc ataggtccgc ttcacgcgct
     4741 cgtattcgtg gacggcgatg agctgtttgt cgtagagccc cttggtgcgg tcgtactcgt
     4801 ccttcaggtt ctgcagctcg agctcgcgct gttcgatctg ccgctcggcc tccatgagct
     4861 cgcgcggcgt gggattcggc tggagctcga gcagcggatc gcccgcccgc acgaactccc
     4921 cggcctccgc gaactggcgc ttgaccacgc ccgagacctg cgacttgacc tcgatctcca
     4981 ccctcggctc gatggtgccc acggccaggg cacggtccac gatggagccc cgcgtcacct
     5041 cgacgtgcgg cctcgcctct tcgtccgagt gcccgttgcg cctgatgaca aatgcggtga
     5101 cggcggcggc gactacgagc gcgccggcaa tgaacaggag tctcttcatg ggaggacggt
     5161 tcgggacgct gtgaatcagc atcagcgtac ggacgcccgg atgccgtgtt gcgctccgtc
     5221 gggccgagcg cctcctcaag ctcggcccgg acaaaccgaa tcgatcaact ggatccaggc
     5281 cgtctgtacg gcgtccgggt cgaatcgtgc gacgaaggtg cgccgcgcac catcggaaag
     5341 acgaagcagc cgcgttcggt ccgtaagggc gctgatcgcg cgggcgaggg ccgcaggatc
     5401 acgcggagga acgacaatgc cgttctcacc atcctcgatc aggtcggcaa gtccaccctg
     5461 tcgggtcccg acgactggca tactccacgc aaacgcctcg aggatggcga gtggcatgcc
     5521 ctctgtggga tggtaggtcg gcagaaggag cgcatcggac gactggtaga gcctccggat
     5581 ctcccctgcc tccgtgacac cacccaggtg ttcgacgcaa tcgtcgagcg cattctccgc
     5641 cacgacctga gcgaatcgct cttcgtcttc cgcccgaacc caccgtcctg cgaatcgaat
     5701 ccgaacgcga tgtccttccc tccgcaccag ggggacagcc cggaggatat ccatccatcc
     5761 cttttcgggc agcatattgc tgacgaacag gagctccagc ctctcgtcag cgtgccgcct
     5821 tgcccgcttc gcctccaggt caccggtcgt ggcgagaagt gcctccgcga tcgtgttcgg
     5881 aatcaccgct cgcttgccgt ccgggatcca cgtagaaacc gactcggaaa tggcgttggt
     5941 cagaaagacg aacactgaag atcgccgttc gatacgcccc atcgtcgctc gcgtgcgccc
     6001 atcctcgaac ctgtcgccga gcgtcgcccg atggctgact gcaatgacgg ggcgcttcct
     6061 tccaaatgac ggcagcacgg agacgacatc gcggacgtgt ccgagcagtg tcggcgaaat
     6121 ggacgtccag acgaccggag ccgccggata ctggctcagc gcccgggtga ggatgaatcg
     6181 ctgtcgcagg taatggagca cgcggccggt acgccaccgc gtctcggccg ccacgaatcc
     6241 ctcgtcgacg ggctcggtgt tgaatcggac tacctggtaa gtcgtctcca gaagttccgc
     6301 gagacgctcg gtcgccacag tctggccatc cttcggggga gggaagcgac cgacaacgat
     6361 tatcgtcgac cgtggatcgg aatgcgctgc tgcgggcatg gactcgcgga caatccgtgg
     6421 agtttaccaa aggaggaccc ggtttggaac cgtttcctcc gggctcgtac tttcgagccc
     6481 cgacgcgaac ggcgaacagg gcggaagggc ggagctcccc accatgaccc agcaggattt
     6541 cgaggtgctg cgggagctcg ctccctggat caactccctg ggccgggtcg gggcgttgtc
     6601 gcccctgcgg gaatgggaag ggcgcctgtt tccccgtgcc gtccgcattc ctcgctggaa
     6661 gtgggatttg aaccggccgt cacgtcgccg tttcgatgtc ctcttggcgg cgaacgtgtt
     6721 catgtactca ccggaccctg cccgttggtt tcgcaacgtg ctcgcgtcgt gtcggtggtt
     6781 tctgctcgtg gacctggttc gccgccggcg gtccgaggtc gacgagttcg gaacggacag
     6841 agacggcatg cgcttcgcgg taggctcgga gcgtccgcgg atcgacggat tcttcgatct
     6901 cgggtccttc ggcgatcgcc ttctcgcctg gcgcacctac tacggaggcg cgaacccgct
     6961 cgacgacgcg ccgcttcacg tcgtcgcgct catccggggc gatctcggcg aaccgattct
     7021 ccgaatcgac gactacccga cgggcgtccg cccgatcctc gacgatatgt cgcccttgca
     7081 cgacatcctg gagcgcgtcg aggcatacga gctttcctac cacctgggca tcgtcccggc
     7141 gctcgtgcag gacgagatgg cctcattcct gcgaagcctg cagcacatgg ttcccgtgat
     7201 gcacggcttc gaccactgtt acccgaaata cagtcgtctg ctcacggagg tcggtgatcc
     7261 gtacaacgag aagaccgtgg gcgtcttcaa cgagttcggg gggaagtcct acgccgagat
     7321 cgtcgcgctg ctttctgatg gacgtgcacg gctcgcggaa gacgtcggga tggagcctcg
     7381 atggtacata cccccgtgca actggggtga ccgcaggaca gggagggcgc ttcaggaggc
     7441 gggattcgat ggctatctct cggagaagcg aatcccggga tgctcacttc catggcacgc
     7501 gagtgatttc tacggtcgat catccggtta cgacctgtcc cgtgagccgg atgtcgtgac
     7561 gctgcatctg acgtgggaat gggacctgct gcgatcgcgg agtcagtcgc ggctggacga
     7621 actgctcacc tcccttgtga gccgccgtct tcaactcgat gggcagatac ggccactgcg
     7681 tgaccgaatc gacgggtgag acgctccttg tccgacgacg gagggggcga tcgatttcag
     7741 ggtctgcctg gtccgccgtg agggcgtgac ctccgaggat cagttcttga gcctccgcgg
     7801 acgcgtggtc catggccacg acgcgttcgg taatgaatcc gcttggctca tcgaaagggt
     7861 tcacaatcgg gatgcccagg cggacgcgac cccgacgggc cgtctgcgaa gaggacgcgg
     7921 ccgaactctt ccagcgtgtg ctcggccgcg aaccggcggg cctcgccggg gtcaaatatc
     7981 ctgtccggaa ggtcccgatt caggacacgg gctagcgcct ccgcgatcgc tgatggctct
     8041 tctgcatctg ccacgacccc gagtttgcgg tcccgcacgt aatgcccgac caggccccag
     8101 tcggaggcca ctaccggcac tccctccgcg gccgcgcgga tgagcacgcc gctggatccg
     8161 atatgccgct ggtacgccac cagtacgacg tcggacgcac ggatcacgtc ctggatgtcg
     8221 tcgtcgccga cgtactggtt gcgtaccacg atctgcgggc cggccagcga tctcgctgcc
     8281 tcgatggaac gggcgatctc ggcagactcg ctctcccagt gtgggcccgc aatgaccagg
     8341 cagaaccggt cttgaagccg gtcgggaagt aggctcaatg accgcagaac tgcatggata
     8401 ccttttcggg ctccgatact cccgaagaag agtccgatcc gtcgatgcgg ttccacgccc
     8461 cattcacgtc gtatctcgtc cgcggtgcgg ccacgggata cccgctggaa tgcgtccggc
     8521 agcgcgacgg actcgactcg cggcgccaac ttccgcagtt caggcacgac gtacggatcg
     8581 agagggtgcc atcgatccac gtggggggtt gctcaacgcc agacggagaa tagtgcgctt
     8641 ccgaaccgcc gacaggcgtg agacagggga gccgaagagg gatcgataat gaaaagaggg
     8701 cccgaagtag attcccgaga agcgcaaggg atagtcgaat cgcaatccga gtccgagcga
     8761 gagctgcgcg tggtccgcaa acatgagtat cacgcggtcg ggcttttccg attctacgag
     8821 ccggcgcagg attcgtccgt gctcgacgtc cgagcggacg agcgaaccca tgagccccag
     8881 ccaccgcgcc ggattctcca gcagcgggag cggaaccatc cgaatgccgt cacgctcatg
     8941 atctgcaagc cagcccgtga tctccgggtg ggcatcgaac atcgccccgg gcgctgctac
     9001 gccgagccgc cccttctcac ctcgatcgag ccagacacgg gcggtttgca gcaggtacga
     9061 cggatggtgc ccgctggcat acaggtcgaa caggatgcga tacggaggca cgtttgcagg
     9121 atccgttcag gcccgtgatg atcgcgagct cgttcgcgct cgaaggatgg cgagcagtac
     9181 gcctccggtc atccccgggt ccgcgacagg gttctgatgc gaggaatcca tgaggtagcc
     9241 agggggtaca tcccgagttt gccccttctt cgacgtggct gtatgatcgg ggccgcgcgc
     9301 cggtcgatcc cgatccacgc ggagcgtgcc caccctatct tccctccatg cactcggcaa
     9361 ttcgatccat ggcacaagaa cgcccacccg tcttcctgga gcaccggagc aacgtccatt
     9421 cgcagttcgg cgaggatggg atcgtcgaat ggatactgga gcaactcgat ctttcgaacg
     9481 gcttctttgt cgagttcgga gcctgggacg ggctgtatct gagcaattgc cgaaggcttt
     9541 acgaacgagg ctggtcggga tgcttcatcg agggcgatgc gcgccggttt cgggacctcg
     9601 agcggacata tgccggaagc gatcgcgccg tgctcgtgca ggcatttgtt tctccggaag
     9661 gcgatacccg actcgacgcc ctgctccgcc ggtcggaggt cgagcgggtg gacctgctct
     9721 cgatcgacat cgacagcgac gatcttgcga tctggcagag tgtccacgag tatcgagcca
     9781 agtgcgtcgt catcgagtac aacccgacga ttccgttcga cacgcgcttt cagaatccgc
     9841 ggggtcggaa cgtgggcaat tccgctctgt cgatcgtgga gcacgcccac gaaatcggct
     9901 acgacctggt tgccggcacc tatacgaatc tcgtgttcgt ggactcgtcg gccaggccgg
     9961 agtccattcc caccgtgacg ctgcagcaga tcaaggatta tgcagagggt gcctttcggt
    10021 atttctttgc ctatgacggc accctgatgc gggaatcatt caacacgatt cgcgagacgg
    10081 agctgtttcg gattccgtgg tcaggggcgg tgggtatcca gccgttgccc cgcatactcc
    10141 gccggttcga tcgactgaac ggcgtgaaga aggtgctgaa cggactcgtg gccatcgtcc
    10201 ttcgacctgt cgggtcggtc cggatgctca tgtcgattcg cggcgatcga cgcgaaccga
    10261 tgtcatgagg cattttgacc agcgagcgtg gtacggggcg gaatccggcc gggccggggg
    10321 tggaaggcag tgaacgtacg ccgcctcctg ctgaatgccg gatccgcctc gttccaggtg
    10381 atcctctcgg caatcgtgct gttcgtggtc tacgggtaca ttgtccgcga gctgggaatc
    10441 gacctctttg gcgtctgggc gacggtggcg gcctccacgt ccgtcgcggc gatcggcggc
    10501 ctcggactcg ccggtggcgt cgtgaagttc gtcgccaagt accgggccct gggcgatctt
    10561 cgtcgcgtgt cgatcgtgct atcgacctcc atggtggcgg ccgcgatcgc cctcggcatc
    10621 atggccgcgc tgggactcct cctgttgccg agcgtactgc catggctcat ggatcggccg
    10681 gatgagctcg aggtgggcat cgacctcgtg ccgtacgtgg tcgtctcctt ctggttgacc
    10741 tcggtatcgg cgattggcgt ttccggcctt gacgggttca accgtatcga cattcgtgca
    10801 gcggtatatg tgggcgccat gatcgtctac gcgatgctgg tgttcatcct gcttcccagg
    10861 cacggaatca tcggccttgt ggaggcgcag atcgtccagg gagtgctgct cctcgtggtg
    10921 ccgtggatcg tgctgggggt gctgacacgg caccggggca tgcttccgct aaggtggtcg
    10981 aacgacgtgt tccgcgaggt cgtcggttac ggcgtcagct tccaggtcat cggaatcgcc
    11041 cagatcctct tcgagcccac cacgaaggcc ctgctgacca agttcggcgg ggcgggggcg
    11101 accggcattt ttgagatggc gaatcgactc gcttttcagg cgcggtccgt aatcgatgcg
    11161 tcacaccgct ccatcgtgcc atcactcgcg gagttgcacg agttggacgc gccggtcctg
    11221 ccggcgattt acacgcgctc ggccaagctg ctcgtaatgg tttcggcctg cctgttgccg
    11281 acgctcgcgg tcctcatgcc ggtcgtgtct gtcgcctggc tcgggacact ggacatcacc
    11341 ttcgtggtcg tctccgtgct gctgtcgact gcgtggatgc tgaacctcct ggcgaccccg
    11401 gcctattacg cgaacctggg tacaggcgcc ctgcgatgga acgtgatcgg cacggttgtg
    11461 acggcgatca tgaacatctc tctaggcttc acgctcgggc gatggtgggg tcccggcggg
    11521 gtcgtcgcgg tcttcgtgct gagcctgctc gtcgggaatg cgttgatggt gtgggattat
    11581 caccgaaggc atccgggctt cgcggaggcg ttcgtcgagc cgggggacat gccgctcgtc
    11641 gtgggctcga tcatcattgc actggcgggg gcgctgctcg gaccgatggc ccttgccgcc
    11701 acgacaccga tcgcatatct catcgcggcg atcgtcttgc tcggcgcctc tttgctgccg
    11761 cttgcgatgc gccactccgg ctaccgcctg cttcgcggcc tcacccgggc cctcgaccgt
    11821 cgctagcttc tggcgtggtt ccaggccggt ccgccgctcg tgtccgatcg aacgccccgg
    11881 cggccagccc cgagaggatg gcaaacatga acaccggatc atcgatgttg aacggattcg
    11941 aggtgatggc cgccagggtc agtgaaacca ggaaggcggc gctggtcagg gcgctcagcc
    12001 tgtaaacgaa ccgtccggat gatcgatagg ccgcgatgcc ccgggtgatc attgtgcccc
    12061 agaaatacag catcatgccg aggccccaga ggccgaactt gtaccacagc gccaggtaac
    12121 cattgtgcag gaatgacttc accaaggtct tgggcggaat gaactggaca tcgtagaagc
    12181 ggaacgggac acccatcccg tgtcccaaca ccgggttgtt cacgaccttt tccagcaccg
    12241 ctgccgactc gcgccagcgg ttcaccaggg acatgtcctc ggttgtggcc gtt
//
LOCUS       DBNHQZ010000288         3924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1169996, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000288 DBNHQZ010000000
VERSION     DBNHQZ010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1169996"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="AC16YU_07805"
     CDS             complement(<1..1458)
                     /locus_tag="AC16YU_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6065167.1"
                     /translation="MIRFLTIADQLRGYDRNTFRSDLTAGLTVGVMLIPQGMAYALIA
                     GLPPIYGLYAALAPLVVYALLGTSRQLAVGPVAMVSLLVLAGVTPLAGDDPGRYLALA
                     LSLALMVGAIQFVLGLARFGFLTNFLSHPVLSGFTSAAALIIGFSQLKHLLGVPLPSS
                     RYAHEILLEALASLGNVHAATLAIGILSILVLVALRRWKKTFPGALVVVALGTATVWI
                     SGLHDSGVRIVGAIPGGLPVPAFPGIGLSDLSALLPTALAVSLVGFMESIAVAKAFAA
                     KHRYRVDANRELVALGASNMVGALFQAFPTTGGFSRTAVNDQAGARTTVASLISAGVI
                     ALTLLFLTPLFRFLPQAVLAAIIVVAVAGLFDVREMRYLWRVKRTDFALLVVTFLATL
                     ALGIEEGILVGVVASLVLVIHETSRPHTAILGRLPGTDIYRNVARNPHAVTRPDVAVV
                     RVDAALYFGNVEYVRDRVLSAVGGSDAVHTVIFDAY"
     gene            complement(1532..2983)
                     /locus_tag="AC16YU_07810"
     CDS             complement(1532..2983)
                     /locus_tag="AC16YU_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6065168.1"
                     /translation="MLFRQIWDDKLAQYAYLIGCQATKEALVIDPERDIDQYIRLAEK
                     EGLEIVAVADTHIHADYVSGMREFAERGIRVYASDEGDADWKYEWLIGSRYDHRLLKD
                     GDTFMVGNIEIKALHTPGHTPEHLSFLVTDKGGGADRPIGIATGDFVFVGDLGRPDLL
                     ESAAGIQGMMEPSARTLYGSVQRFLDLEDHIQVWPAHGAGSACGKSLGAVPQSTVGYE
                     RQFNGAVQSALRGEDEFVSAILAGQPEPPMYFARMKRDNKIGPKVLHSLPNPYRLSIG
                     ELGKLSGRTDIVVIDARVDRESFMAGHLAGSICAPFNKAFNTVVGSFVEENTPIFLII
                     QEAHVEAAVRDLVRIGLDDIVAYATPEMIDRYRDAGGELAHIPEMSIEDVATLSKRDD
                     VLVLDVRNATEHEAARVPGSFNIAHTRLWVRRDEVPTGKTLAVHCMTGGRSAASASLL
                     KRLGRDVVFLTGDFSVWASRNEVASGDPALANA"
     gene            complement(3025..3471)
                     /locus_tag="AC16YU_07815"
     CDS             complement(3025..3471)
                     /locus_tag="AC16YU_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR6065169.1"
                     /translation="MTTASTASPSRRLGAYLLLGAVLGLVFTKSEVISWFRIQEMFRF
                     QSFHMYGIIFSAIIVGMISVHLIKRRQTRDLDGNPIEIPPKEMGRGTRYWLGGTLFGL
                     GWALTGACPGPMFALVGNGLLVILVALFAAIAGTWVYGWLRPSLPH"
     gene            complement(3480..>3924)
                     /locus_tag="AC16YU_07820"
     CDS             complement(3480..>3924)
                     /locus_tag="AC16YU_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR6065170.1"
                     /translation="RHICAMSAPAGVPFFDYDWKSSGLWNLTFVVGVTMGAFLTAYLL
                     PGDGTVAVSEATRSDLAALGIVDFDGLAPRDIFSVESLLSLKGFVSVVVGGFLVGFGA
                     RYAGGCTSGHAISGLSNLQLPSMLAVAGFFVGGLITTWVLLPLLF"
BASE COUNT          703 a         1242 c         1421 g          558 t
ORIGIN      
        1 ataggcgtcg aatatgacgg tatggaccgc gtcggagccg ccgacggcgg agaggacgcg
       61 gtcccgcacg tactcgacgt tgccgaagta gagcgcggcg tccacgcgga cgacggccac
      121 gtcgggccgc gtcaccgcgt gcgggttgcg cgccacgttc cggtagatgt cggtgcccgg
      181 aaggcgtccg aggatcgccg tatgcggccg cgaggtttcg tggatcacca ggacgagcga
      241 cgccacgacg ccgacgagga tgccctcttc gatgccgagc gcgagcgtgg cgaggaacgt
      301 gacgaccagg agcgcgaaat ccgtgcgctt cacccgccac aggtaccgca tctcccggac
      361 gtcgaagagc ccggccaccg ccacgacgat gatggccgcc agcacggcct ggggcaggaa
      421 ccggaagagg ggcgtgagga agagcagggt cagcgcgatc acgccggcgc tgatgagtga
      481 cgctacggtc gtccgggcgc cggcctggtc gttgacggcg gtccgcgaga agccgccggt
      541 cgtcgggaag gcctggaaga gcgcgcccac catgttggac gcgccgaggg ccaccagttc
      601 gcggttcgcg tccacgcggt agcggtgctt ggccgcgaat gccttggcga cggcgatcga
      661 ctccatgaag ccgacgagcg agacggcgag ggcggtcgga agaagggccg acaggtcgga
      721 gaggccgatg cccgggaatg ccgggaccgg aaggccgccc ggaattgcgc ccacgatgcg
      781 cacgccgctg tcgtgcagcc ccgagatcca gaccgtggcg gtgcccaggg ccacgacgac
      841 gagcgctccg ggaaacgtct tcttccagcg gcggagcgcg accagaacca ggatggacag
      901 gatcccgatg gcgagggtgg cggcgtgcac gtttccgagg gacgccagcg cctcgaggag
      961 gatctcgtgg gcgtacctgg acgagggcag cggcaccccc aggaggtgct tgagctggct
     1021 gaagccgatg atgagggcgg cggcgctcgt gaagccgctc agcaccgggt gcgaaaggaa
     1081 attggtcagg aagccgaagc gggccagtcc gagcacgaac tggatggcgc ccaccatcag
     1141 cgcgagcgac agggcgaggg ccaggtaccg gccggggtcg tccccggcga ggggcgtcac
     1201 gccggcgaga accagcaggg agaccatcgc gaccggtccg accgccagct gcctggacgt
     1261 tccgagcagc gcgtagacga cgagcggcgc gagcgccgcg tacagtccgt agatcggcgg
     1321 aagcccggca atcagggcgt acgccatccc ctgcgggatg agcatgacgc cgaccgtcag
     1381 gcccgccgtg agatccgacc ggaacgtgtt gcggtcgtat ccccggagct ggtcggcgat
     1441 ggtcaggaag cggatcacgg tgtcagggtt cgggtcgccg ggagcggggc gcggccgccg
     1501 gacggaccgg cggccgcagc accccggttc gtcaggcatt cgcgagggcg gggtctcccg
     1561 aggcgacctc gttgcgggaa gcccacacgc tgaagtcgcc cgtcaggaac acgacgtcgc
     1621 ggcccaggcg cttcaggagc gaggctgacg ccgccgaccg accgccggtc atgcagtgca
     1681 cggccagcgt cttcccggtg ggaacctcgt cgcgacgcac ccacaggcgc gtgtgcgcga
     1741 tgttgaacga tccgggcacg cgggcggcct cgtgctcggt cgcattccgc acgtcgagga
     1801 ccagcacgtc gtcccgcttc gagagggtcg cgacgtcctc gatgctcatc tccgggatgt
     1861 gggcgagctc ccctccggcg tcgcggtagc ggtcgatcat ctcgggcgtg gcgtaggcca
     1921 cgatgtcgtc cagcccgatg cgcacgagat cccgcacggc ggcctcgacg tgcgcctcct
     1981 ggatgatcag gaagatgggc gtgttctcct cgacgaacga gcccaccacc gtgttgaacg
     2041 ccttgttgaa gggcgcgcag atcgagccgg cgaggtgacc ggccatgaag gactcgcggt
     2101 ccacgcgggc gtcgatcacg acgatgtccg tgcgtcccga gagcttgccc agctccccga
     2161 tcgagagtcg gtacgggttg ggcaggctgt gcaggacctt cggcccgatc ttgttgtcgc
     2221 gcttcatgcg cgcgaagtac atcggcggtt cgggctggcc ggcgaggatg gccgagacga
     2281 actcgtcctc gccccggagg gccgactgca ccgcgccgtt gaactgccgc tcgtatccga
     2341 ccgtcgactg cggaaccgcc ccgagggact tcccgcacgc cgagcccgcg ccgtgggcgg
     2401 gccacacctg gatgtggtcc tccaggtcca ggaaccgctg caccgaaccg tagagggtgc
     2461 gtgcggacgg ttccatcatt ccctggatgc cggcggcgga ttccagcagg tcgggacggc
     2521 ccaggtcccc gacgaatacg aagtcgccgg tcgcgattcc gatcgggcga tccgcccctc
     2581 cccccttgtc cgtcacgagg aagctcaggt gctccggcgt gtgccccggc gtgtgaaggg
     2641 ccttgatctc gatgttgccc accatgaacg tgtccccgtc cttcagcagg cgatgatcgt
     2701 accggcttcc gatcagccac tcgtatttcc agtcggcatc tccctcgtcg gatgcgtaca
     2761 ccctgattcc gcgctcggcg aactcgcgca tcccggagac atagtccgca tggatgtggg
     2821 tgtcggcgac ggccacgatc tcgaggccct ccttctccgc gaggcggatg tactgatcga
     2881 tgtcccgttc gggatcgatc acgagggctt ccttggtggc ctgacagccg atgaggtagg
     2941 cgtactgggc gagcttgtcg tcccagatct ggcggaacag catggcagta gtgggttggt
     3001 gcgggtgaag gggtgaggcg ggaatcagtg cgggaggctg ggacggagcc agccatacac
     3061 ccaggtgccg gcgatggcgg cgaacagggc gacgaggatg accaggaggc cgttgcccac
     3121 gagagcgaac atgggtccgg ggcaggcgcc ggtaagcgcc catcccagcc cgaaaagtgt
     3181 tccgccgagc cagtagcggg taccgcgtcc catctccttg ggcggaatct cgatcggatt
     3241 gccgtccaga tccctggtct gtcgacgctt gatgaggtga acggagatca tgcccactat
     3301 gatggcggaa aagatgatcc cgtacatgtg gaaggactgg aaccggaaca tctcctggat
     3361 gcggaaccag gaaatgacct cgctcttcgt gaagacgagg cccagcacgg cgcccaggag
     3421 caggtaggcc ccgagtcggc gagagggtga tgcggtcgaa gcggtcgtca tgtgcgtgat
     3481 cagaaaagga ggggcagcaa cacccacgtg gtgatcagcc cgccgacgaa gaagccggcg
     3541 acggccagca tcgagggaag ctgcaggttc gagaggccgg aaatcgcgtg cccggaggtg
     3601 cagcccccgg cgtagcgcgc gccgaagccc acgaggaagc ctccgacgac gacgctgacg
     3661 aagcccttca gcgagaggag gctctcgacc gagaagatgt cgcgcggtgc gagcccgtcg
     3721 aagtccacga tgccgagcgc ggcgagatcg gaccgcgtgg cctccgagac ggccacggtt
     3781 ccgtcacccg gaagcagata ggcggtgagg aaagcgccca tcgtgacgcc cacgacgaag
     3841 gtcagattcc acaggccgct cgatttccag tcgtaatcga aaaacgggac gccggcggga
     3901 gcgctcatcg cacagatgtg gcgg
//
LOCUS       DBNHQZ010000289         2706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2660686, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000289 DBNHQZ010000000
VERSION     DBNHQZ010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2660686"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="AC16YU_07825"
     CDS             <1..379
                     /locus_tag="AC16YU_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HIR6065171.1"
                     /translation="DQDGVFVWSFSLPNALYGDPYDEGDTATHEVGHWLGLYHTFQAS
                     GTGRPQNRTGCAPPGDRVDDTPAEASAAFGCPVGRDTCPAQGLDPIYNFMDYTDDACM
                     YEFTPGQGDRMTGTWAAFRAPAT"
     gene            complement(445..517)
                     /locus_tag="AC16YU_07830"
     tRNA            complement(445..517)
                     /locus_tag="AC16YU_07830"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Phe,seq:gaa)
     gene            complement(613..1488)
                     /locus_tag="AC16YU_07835"
     CDS             complement(613..1488)
                     /locus_tag="AC16YU_07835"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6065172.1"
                     /translation="MVFGITGNTLKANLWAPVEQVIGELRQLGHPFRLHAAVAAGLLD
                     RGFLSPDEARELRSDGLSESCELLLSFGGDGTLLNTAAEVGDRGTPILGVNIGRLGFL
                     AHMEAGHLSAALHRLAHGEYVTEPRMVLEADAGDTSLPNRWALNECVIQRTGAAGLLS
                     IEVRIDGRELNTYWADGIIVATPTGSTAYNLAVGGPIVSPGSGTIVLTPLAPHTLTVR
                     PVVVPDDVVIEARVVDPDAPYVFTLDGRGTTIPGSRTILTIRRAAHTVNLVAMPEHEF
                     FSTLRSKLMWGARKT"
     gene            1611..>2706
                     /locus_tag="AC16YU_07840"
     CDS             1611..>2706
                     /locus_tag="AC16YU_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6065173.1"
                     /translation="MTDAPASHRIARAWSATTIGIEAIPVEVEVHVRTGLPSYTVVGL
                     PQATVRESRDRVLSALRASGLPIPRGAVTVSLAPADVRKEGALFDLPLAVALLAAQGV
                     VPAAGRASKVPVIGELALDGRVRPVRGVLAVAMSARERGTREVVVPRGNAAEAAAVDG
                     LQVYGVSSLAEAVSVLRGFGSPWVGSVPPERDHGPEPDMADVAGQAVARRALEVAAAG
                     RHNVLLVGPPGVGKSMLARRLPGLMPELSGSEALEASRVHSVAGLLEPGTGLLRRPPF
                     RAPHHTVTVAGMIGGGDPARPGELSLAHHGVLFLDELPEFRRRVLEALRQPVEDGLVS
                     VSRARSRVTLPADFLLIASMNPCPCGRGGTP"
BASE COUNT          378 a          917 c          993 g          418 t
ORIGIN      
        1 ggaccaggac ggcgtcttcg tctggtcgtt ctcccttccg aacgccctgt acggcgatcc
       61 gtacgatgag ggcgacacgg cgacccacga ggtggggcac tggcttggac tctaccacac
      121 cttccaggcg agcgggaccg ggcggcctca gaaccgcacc ggctgcgcgc caccgggaga
      181 ccgggtcgac gatacgccgg cggaggcttc cgcggccttc gggtgcccgg tggggaggga
      241 tacgtgcccc gcgcaggggc tggatcccat ctacaacttc atggactaca cggacgacgc
      301 gtgcatgtac gagttcacgc ccggccaggg cgaccgcatg accggcacgt gggcggcgtt
      361 cagggcgccg gccacctgac cgggagcacg ccggagaatg aagaagaccc tgccggcctc
      421 gtgccggcag ggtctttcga cgggtggtca gggccggaat cgaaccggcg acaccaggat
      481 tttcagtcct gtgctctacc tactgagcta cctgacctca aagggcgtcg aatatactga
      541 ccccgattcc gggcagtcaa tcgaacgcgc ggcccgtccg ggtgattcac gcgatgttgg
      601 gggctccgcc ggtcaggtct tccgcgcgcc ccacatcagc ttggagcgga gcgtggagaa
      661 gaactcgtgc tcgggcatgg ccacgaggtt caccgtgtgg gccgcccggc ggatggtcag
      721 gatcgtccgg ctgcccggta tggtcgtacc gcgtccgtcc agcgtgaaga cgtacggggc
      781 gtccggatcc accacgcgtg cctcgattac gacgtcgtcc gggaccacga cgggtcggac
      841 ggtgagcgta tgcggggcga gcggcgtgag cacgatcgtc ccgctaccgg ggctcacgat
      901 cggcccgccc acggccaggt tgtacgccgt ggaaccggtc ggcgtggcga cgatgatgcc
      961 gtcggcccaa taggtgttca gctcccggcc gtcgatccgg acctcgatgg acaggagtcc
     1021 cgccgccccg gtccgctgga tcacgcactc gttgagggcc caccggttcg gcagcgacgt
     1081 atctcccgcg tcggcctcga gcaccatgcg gggctcggtc acgtactcgc cgtgggccag
     1141 gcgatgcagc gccgccgaca ggtgcccggc ttccatgtgc gccaggaatc cgaggcggcc
     1201 gatgttgact ccaaggatgg gcgttccgcg atcaccgacc tcggccgccg tgttcagaag
     1261 cgtgccgtcc cctccgaacg agagcaggag ttcacacgat tccgaaagcc cgtccgagcg
     1321 cagttcgcgc gcctcgtctg gcgagagaaa gccccgatcc aggagtccgg ccgcgaccgc
     1381 cgcgtgaagg cggaacgggt gcccgagctg tcgcagctcg ccgatcacct gctcgaccgg
     1441 ggcccagagg ttggccttga gggtgtttcc ggtgatcccg aataccatgg cgggcgcagg
     1501 tgacgggccg caaggtacga ccggggcggc cgcgggtcca cgagcacgtc ccgtccatgc
     1561 ggcggacctg cggcgggtta tggccggccc gccggcgggg tcggaggagt gtgaccgacg
     1621 ctccggcttc ccatcgcatc gcgcgcgcat ggagcgcgac caccatcgga atcgaggcga
     1681 ttcccgtgga ggtggaggtc cacgtacgga ccggacttcc atcgtacacg gtggtcgggc
     1741 tgcctcaggc cacggtgcgc gaaagccgcg accgggtgct ttcggccctc cgcgcttcgg
     1801 ggctgccgat cccccgcgga gcagtgaccg tcagcctcgc gccggcggac gtgcgcaagg
     1861 agggcgcgct gttcgatctg ccgctggccg tggcgctcct ggccgcgcag ggtgtcgttc
     1921 cggccgcggg gcgcgcctcg aaggtcccgg tcatcgggga gctggcgctc gacggccggg
     1981 tccgcccggt ccggggcgta ctcgccgtgg cgatgtcggc gcgggagagg gggacgcgtg
     2041 aggtggtcgt tccgcggggg aacgcggcgg aggcagcggc cgtcgacggt ctgcaggtgt
     2101 acggcgtgtc cagcctggca gaagccgtct ccgttctccg gggattcggc tcgccctggg
     2161 tcgggtccgt cccgcccgag cgcgaccacg gtccggagcc cgacatggcg gacgtggccg
     2221 gccaggccgt agccaggcgg gccctcgaag tggccgccgc cggtcggcac aacgtgctcc
     2281 tcgtgggccc gccgggcgtc gggaagagca tgctggcccg acgccttccg ggcctgatgc
     2341 cggagctttc gggcagcgag gctctcgagg cttcccgggt gcattcggtg gccggactcc
     2401 tcgagccggg cacgggcctg ttgcgcaggc cgccgttccg tgccccgcat catacggtga
     2461 ccgtggcggg gatgatcggc ggcggagatc cggcaaggcc cggcgagctg tcgcttgccc
     2521 atcacggtgt tctcttcctg gacgagcttc ccgagttccg ccgccgggtg ctcgaggccc
     2581 tgcgtcagcc tgtcgaggac ggcctcgtct ccgtgtcgag agcccgttcg cgggtaacgc
     2641 tgccggcgga tttcctcctg atcgcgagca tgaacccgtg cccgtgcggc cggggcggca
     2701 cgcccg
//
LOCUS       DBNHQZ010000290         2041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_358240, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000290 DBNHQZ010000000
VERSION     DBNHQZ010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_358240"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..848
                     /locus_tag="AC16YU_07845"
     CDS             159..848
                     /locus_tag="AC16YU_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065174.1"
                     /translation="MKTFIPLAAMAVVLTWAAANPNQAIDGARAAGRYVKSLPHKQVD
                     VSEWKRFDDPFARHDWAFERYERYQAEIRDLLKRAQGGQDADAMYTVGTWHLGTQQYP
                     NFGARDPEMGRQMLRQAASLGHSMAAFMIWEMDDRTTPTLRRISGEWTGEFDNRAAIE
                     LAMRARNACDLRLMEEALDALTPYAGKLPFTHPVGPGAATARANLWKHDLIVSLQDLA
                     MFRRYYMQRCT"
     gene            913..1785
                     /locus_tag="AC16YU_07850"
     CDS             913..1785
                     /locus_tag="AC16YU_07850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6065175.1"
                     /translation="MDAFIAFFEDIPTSYRTAILVGGFMLFWLLEGAVPLFRFEFRRG
                     THALVNLLFWSFTLAINFGFAFLIVGSADFTTAHRAGLLYLVDLPAWARVLAGLLLLD
                     LVSAWLIHWIEHRVRWMWKFHLVHHSDRQVDVTTGLRHHPGEALFRAAFTVLAILVAG
                     VPVAVVMLYQTLSALFAQLTHANVRVPDRLDRVLSWVFVTPNMHKVHHHFAQPLTDTN
                     YGNIFAVWDRLFGTFAYAPADRLTYGIDTHMGPGEDSRVGGLLAIPFRPYRPPTGAKF
                     ADAREGAADPPEAG"
     gene            complement(1816..>2041)
                     /locus_tag="AC16YU_07855"
     CDS             complement(1816..>2041)
                     /locus_tag="AC16YU_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065176.1"
                     /translation="WIFDRDTRLGLEDRLSYRIINQWVISEHKSQGTMQMYAGFGRYE
                     LYWLYAMNTPGALGRADSLLEAAGLARADR"
BASE COUNT          308 a          709 c          673 g          351 t
ORIGIN      
        1 cttcttaagc ggcctgcggt cctttttttg ggggatcggg gaggtggctc catgcctgca
       61 cgcgtttaag cgcggcggcg ccgcgacgat tatccgagaa gagtcacacc ctcgcggcgg
      121 cgtgacggtg tccgttccgc cgaagcccaa cgacgcccgt gaaaaccttt atccccctgg
      181 ccgccatggc agtcgtcctg acctgggcgg cggcgaatcc caaccaggcg atcgacggcg
      241 cccgcgccgc ggggcggtac gtcaagagcc tgccgcacaa gcaggtggac gtttccgaat
      301 ggaagcgttt cgacgatccg ttcgcccgcc acgactgggc attcgagcgg tatgagcggt
      361 accaggcgga gattcgcgac ctgctcaagc gggcgcaggg tggccaggat gcggacgcca
      421 tgtacaccgt aggcacgtgg cacctgggaa cgcagcagta tccgaatttc ggcgcgaggg
      481 acccggagat gggcaggcag atgctccggc aggcggcctc gctcgggcac agcatggcgg
      541 ccttcatgat ctgggaaatg gacgaccgga cgacgcccac gctgcgccgc atttctggcg
      601 agtggaccgg ggagttcgac aaccgggccg ccatcgagct ggccatgcgc gcacgcaacg
      661 cgtgcgacct ccgcctgatg gaagaggccc tggacgcgct cacgccgtac gcgggcaagc
      721 tgcccttcac gcatcccgtg gggcccggcg cggcgaccgc ccgcgccaat ctctggaagc
      781 acgacctgat cgtctcgctg caggacctcg cgatgttccg gcgctactac atgcagcgct
      841 gcacgtgagc cggaccgggg aactccgcgt ccacgcgtga catcgaagcg gcccggccct
      901 tccgcccgcc cgatggacgc cttcatcgcc ttcttcgagg acatccccac gtcatacagg
      961 acggcgatcc tcgtgggggg cttcatgctg ttctggctcc tggaaggggc cgttccgctc
     1021 ttccggttcg agttccgccg cggcacccat gcgctggtga acctcctgtt ctggagcttc
     1081 accctcgcga tcaacttcgg cttcgccttc ctcatcgtcg gctcggccga cttcaccacg
     1141 gcgcatcgcg cggggctgct gtatctcgtc gatctcccgg cctgggcgcg cgtgctcgcc
     1201 gggcttctgc tcctcgacct cgtgagcgcg tggctcatcc actggatcga acaccgggtc
     1261 cggtggatgt ggaagttcca cctggtccat cactcggacc ggcaggtgga cgtgacgacg
     1321 gggctccgtc atcatccggg tgaggcgctg ttccgggcgg ccttcacggt gctggccatc
     1381 ctcgtggcag gcgtcccggt cgcggtcgtg atgctgtacc agacgctctc ggcgctcttc
     1441 gcccagctca cgcacgccaa cgtccgggtg cccgaccgcc tggaccgggt gctgtcgtgg
     1501 gtcttcgtca cccccaacat gcacaaggtg caccaccact tcgcgcagcc gcttacggac
     1561 acgaattacg gaaacatctt cgccgtgtgg gaccggctct tcgggacgtt cgcctacgcc
     1621 ccggccgacc gcctcacgta cgggatcgac acgcacatgg gccccgggga ggattcacgc
     1681 gtcggaggcc tgctggcgat cccgttccgg ccctaccggc ctccgacggg ggcgaagttc
     1741 gcggatgccc gcgagggagc ggccgacccg cccgaagccg ggtgagttcg gggccgcact
     1801 cccgtcgggc ggggttcacc ggtccgcgcg cgcgaggccg gctgcctcca ggagcgaatc
     1861 tgcccggccg agcgccccgg gcgtgttcat cgcgtagagc cagtaaagtt cgtaccggcc
     1921 gaagccggcg tacatctgca tcgtgccctg cgacttgtgt tcggaaatga cccactggtt
     1981 gatgatccgg tagctcaggc ggtcctccag cccgagccgc gtatcccggt cgaagatcca
     2041 g
//
LOCUS       DBNHQZ010000291         4365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_222743, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000291 DBNHQZ010000000
VERSION     DBNHQZ010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_222743"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="AC16YU_07860"
     CDS             complement(<1..399)
                     /locus_tag="AC16YU_07860"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6065177.1"
                     /translation="MTDPFLHEGPDGEPRCWWVRFAPEFVLYHDREWGYPVADDRRLF
                     EKMSLESFQSGLSWRTILNKREAFRAAFDGFEFGRVAGYDDRDVERLLGDAGIVRHRG
                     KIEATIHNAAVARDLVDRYGSLAAFVWRFEP"
     gene            465..1088
                     /locus_tag="AC16YU_07865"
     CDS             465..1088
                     /locus_tag="AC16YU_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065178.1"
                     /translation="MFQRLPVRYLPRILLALAVAAAVVVWSIPPEAKLGGAIRMVLLH
                     GALARTGIVMLGLAAMAGAVMLILRRDPLAALNRTMQASALGTWVVYAVSSVAATWMT
                     WGVPVAWDEPRTQASARILAMVVVFFFVTRRIGQPVLTAFANLVAGLAAFLLIRSAGA
                     VQHPVDPVMGADAAIFPVLFVTLWGILVAMALTAARWLHVRTVPGQG"
     gene            complement(1151..2605)
                     /locus_tag="AC16YU_07870"
     CDS             complement(1151..2605)
                     /locus_tag="AC16YU_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6065179.1"
                     /translation="MDTVFYKRFERHDGAAGLKAHATHYCAGCGHGLAHRFVAEAIES
                     LGIQDRTIAISPVGCSVFLYYYLDVGNTQAAHGRAPAVAIGHKLANPDSIVISYQGDG
                     DLASIGLAEIMHAAQIGLPITVIFVNNAIYGMTGGQMAPTTLVDQRTATSPEGRTRFM
                     GAPLRMAETIAQMDGPIYVERVALFNNKQRFRARKAIEKGIRMQVEGRGFSFVEVLSE
                     CPTHLKLTPEEAEAWVEEHMVPVFPLGVKKDLNPEPWWNPAAPSFEPDDLIGVLGASD
                     VPVPRHAKGFPAHLDAADVGIKLAGAGGDGAQTVAKLLCVAAIEEGFDGTYIPSYGPE
                     SRGGTSYADVHVASDEVLSPAVPDPHVLVAFNAPSLDKFGPTIRENGLALYDSTVIRS
                     VPRLPKGVRAVGVPFTEIAHDIGTIRVKNIVALGALQAATDVFPQETLRGVMARLLHA
                     VPEMRAVNERAFRAGAEAYRKALAGGMLDGVSRN"
     gene            complement(2586..4010)
                     /gene="vorB"
                     /locus_tag="AC16YU_07875"
     CDS             complement(2586..4010)
                     /gene="vorB"
                     /locus_tag="AC16YU_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HIR6065180.1"
                     /translation="MSSATPKTERPRPFLYPQFCKACGRCIEACPNDAIHMGTEIDPR
                     SGLTPVVIDLDKCNGCGLCFEACPEPFGLMPKPDPAFDPMITDPETLLGPKASTAPKP
                     VPIPDRRLPLPAMETMVLKGTHASAVGALLAGCRHFYGYPITPSTEGAELLAKLLPRL
                     EGHFVQAVSEVAAVNMMYGTGAAGLRTMTFTSSPGFSLMLEGISYMIGAEVPAVFVNI
                     MRGGPGLGNIAPEQADIKLVCRGLGHGNTQAISLAPSTPQEMLDLTMLAFELAFKYRN
                     PVIVAGDGYLGQITGQVRLPDHMIRPGIPEWAVYGDAMHRGNLVNSIRLSEAELEHHN
                     EMLVAKYDRMRAEEQRADTFLCDDPEWLVIAANTPARMAKGAVRALRDRGVRAGLFRP
                     VTLWPFPIDRLKPLLGSVRGFVVVEAGSGQLEDEVRLAMSHAGLAMPQVIRVRRMGGV
                     LPSQHEIMDRILTEDEVLHGHGVL"
BASE COUNT          626 a         1516 c         1518 g          705 t
ORIGIN      
        1 cggttcgaag cgccacacga aggcggccag cgagccgtac cggtcgacga gatcgcgcgc
       61 aacggccgcg ttgtggatcg tggcctcgat cttgccccgg tgccggacga tgccggcgtc
      121 gccgagcaga cgctccacgt cccggtcgtc gtacccggcc acgcgcccga attcgaatcc
      181 gtcgaaggcg gcccggaacg cctcgcgctt gttcaggatc gtgcgccagg acagcccgga
      241 ctggaacgac tcgagcgaca tcttctcgaa gagccgccgg tcgtcggcga cggggtatcc
      301 ccactcccgg tcatggtaca ggacgaactc gggagcgaac cggacccacc agcagcgcgg
      361 ctcgccgtcg gggccctcgt gcagaaacgg atcggtcatg ggcgtcgggg aacggtcggg
      421 gggcacgaag gtaaggcgcc ccgcatccga cttcgcccct cgacatgttc cagcgcctgc
      481 cggtccggta cctcccgcgc attctcctgg ccctcgccgt ggcggcggcc gtcgtggtct
      541 ggagcattcc gcccgaggcg aagctcggcg gcgccatccg catggtcctc ctgcacggcg
      601 cgctcgcgcg cacgggcatc gtcatgctcg gacttgccgc gatggcgggg gccgtgatgc
      661 tgatcctgcg ccgcgacccc ctggccgccc tgaaccggac gatgcaggcc agcgcgctcg
      721 gcacctgggt ggtctacgcc gtctcgagcg tggcggccac ctggatgaca tggggtgtcc
      781 cggtcgcgtg ggacgagccg cggacgcagg cgagcgcccg gatcctcgcg atggtcgtgg
      841 tcttcttctt cgtgacccgg cgcatcggtc agccggtcct cacggccttc gccaacctgg
      901 tggcagggct cgccgcgttc ctcctgatcc gttccgccgg cgcggtgcag catcccgtcg
      961 acccggtgat gggtgccgac gccgcaatct tccccgtcct ctttgtcacg ctttggggca
     1021 tcctcgtggc catggcgctc acggcggccc gatggctgca cgtgcggacg gtcccggggc
     1081 agggctgacc ggcgcacctg cccacgggcg agcatccgcg gccgggccgc cccatcagcc
     1141 ggcagcccgg tcagttccgg gatacgccgt cgagcatgcc cccggcgagg gccttgcggt
     1201 aggcttccgc cccggcgcgg aaggcgcgct cgttcacggc ccgcatctcg ggcaccgcgt
     1261 ggagcaggcg ggccatcacg ccccggaggg tttcttgcgg gaagacgtcc gtcgcggcct
     1321 gcagcgcccc gagtgccacg atgttcttca cgcggatcgt cccgatgtcg tgggcgatct
     1381 ccgtgaaggg gacgccgacc gcgcggacgc ccttcggcag gcggggcacc gaacggatca
     1441 cggtggaatc gtacagggcg aggccgttct ccctgatcgt cggcccgaat ttgtccaggc
     1501 tcggggcgtt gaacgcgacc aggacgtggg gatcgggaac ggccggcgag agcacctcgt
     1561 cggaggccac gtgaacgtcc gcgtaggagg ttccgccgcg cgactcgggc ccgtaactgg
     1621 ggatgtaggt cccgtcgaag ccctcctcga tcgcggccac gcacaggagc tttgcgacgg
     1681 tctgggcgcc gtctccgccg gctcccgcca gcttgattcc cacgtcggcc gcatcgaggt
     1741 gagccgggaa gcccttcgcg tggcgcggga cgggcacgtc ggaggcgccc agcacgccga
     1801 tgaggtcgtc gggctcgaac gacggcgccg ccggattcca ccacggctcg ggattgaggt
     1861 ccttcttcac tccgagcggg aagaccggga ccatgtgctc ctcgacccag gcctcggcct
     1921 cctcgggggt cagtttcagg tgggtcgggc actccgagag cacctccacg aacgagaatc
     1981 cgcggccctc cacctgcatc cggatgccct tctcgatggc cttgcgcgcg cggaaacgct
     2041 gcttgttgtt gaagagcgcg acccgctcga cgtagatcgg gccgtccatc tgggcgatcg
     2101 tctccgccat ccggagcggc gcccccatga accgggtgcg tccctcgggc gacgtcgcgg
     2161 tgcgctggtc caccagcgtc gtgggcgcca tctgcccacc cgtcatgccg tagatggcgt
     2221 tgttcacgaa aatgaccgtg atcggcaggc cgatctgggc cgcatgcatg atctcggcca
     2281 gcccgatcga cgccaggtcg ccgtcgccct ggtagctgat gacgatggaa tcggggttgg
     2341 ccagcttgtg tccgatcgcc acggccggcg cccggccatg cgcggcctgg gtgttgccca
     2401 cgtccaggta gtagtacagg aagacggagc atcctaccgg cgagatcgcg atggtccggt
     2461 cctggatgcc caggctctcg atggcctcgg ccacgaacct gtgcgcgagg ccgtgcccgc
     2521 agccggcgca gtagtgcgtc gcgtgggcct tgaggccggc cgcgccgtcg tgccgctcga
     2581 agcgcttata gaacaccgtg tccatgcaga acctcgtcct cggttagaat cctgtccatg
     2641 atctcgtgct gcgacgggag cacgcctccc atcctccgca cgcggatcac ctgcggcatg
     2701 gcgagccccg catgactcat cgcgagccgc acctcgtcct cgagctgccc gcttcccgcc
     2761 tcgacgacga cgaagccgcg gacgctgccc agcagcggct tcaggcggtc gatcgggaac
     2821 ggccacaggg tgaccggccg gaacagcccg gcccggacgc cccggtcccg cagcgcccgc
     2881 accgcgccct tggccatgcg ggccggcgtg ttggcggcga tgaccagcca ctccggatcg
     2941 tcgcacagga aggtgtccgc ccgctgttct tcggcgcgca tccggtcgta cttggcgacg
     3001 agcatctcgt tgtgatgctc cagctcggcc tcggacagcc ggatggagtt gaccaggttg
     3061 ccgcgatgca tggcgtcccc gtagaccgcc cactcgggga tgccaggcct gatcatgtgg
     3121 tcgggcaacc gcacctggcc ggtgatctgt cccaggtatc cgtcccccgc gacgatgacg
     3181 ggattgcggt acttgaatgc gagctcgaag gcgagcatcg tgaggtcgag catctcctgc
     3241 ggcgtcgacg gggccagcga gatggcctgc gtgttgccgt ggcccaggcc gcggcagacg
     3301 agcttgatgt ccgcctgctc cggggcgatg ttgccgaggc ccggtccccc gcgcatgatg
     3361 ttgacgaaca cggccggcac ctcggcgccg atcatgtagg agatgccctc gagcatcagg
     3421 ctgaagccgg gggaagaggt gaacgtcatc gtcctgagac ccgcggcgcc cgtgccgtac
     3481 atcatgttga cggccgccac ctcgctgacg gcctgcacga agtggccctc gagccgcggc
     3541 aggagcttcg cgaggagttc cgcgccctcg gtcgacggcg tgatgggata gccgtagaag
     3601 tgccggcagc cggcgagcag cgccccgacc gccgaggcgt gggtgccctt gagcaccatc
     3661 gtctccatcg ccggaagcgg cagcctgcga tcgggaatcg gtacgggctt cggcgcggtg
     3721 gacgccttcg ggccgaggag cgtctccgga tccgtgatca tgggatcgaa ggccggatcc
     3781 ggcttgggca tgaggccgaa cggctccggg cacgcctcga agcacaggcc gcagccgttg
     3841 catttgtcca ggtcgatcac gaccggagtc agcccggagc gcggatcgat ctcggtgccc
     3901 atgtggatcg cgtcgttcgg acaggcctcg atgcagcgac cgcaggcctt gcagaactgg
     3961 ggatacagga agggcctggg ccgctcggtc ttcggagtgg ctgagctcat ggcgtgcggg
     4021 ggttgcagtc cagggaacgc tcacccatgg tatcggaagc gcttccgccc tgctgcgatg
     4081 cttcgcactt ttcggcctga ggggtccgcc cgacgcgggg tgccggggtt ttccctacgg
     4141 cagtacgggc aatggccgcc cggccgcccg gccgcgggtt ctgcccggcg gcgattactg
     4201 ccaggcctcc aggaatcccg tcgaatcgcc ccgctcctcg tcgatggccg cgtcgaacat
     4261 cgcgcgcagg atctcgtcgg gaaacgtccc gatgaccatc cgcccgttga tgagcatcgt
     4321 cggcgtggac cggatgccgg attcgtactc ggccgacgtc cgcgc
//
LOCUS       DBNHQZ010000292         2650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3340589, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000292 DBNHQZ010000000
VERSION     DBNHQZ010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3340589"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="AC16YU_07880"
     CDS             <1..286
                     /locus_tag="AC16YU_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065181.1"
                     /translation="SLLVWSFFRNDYRLGTQVSLFWLAHNCINISVYAADARLRRLPL
                     LGGDRSEHDWWNLLGMTGLLAYDQVFGWLFFALAIVLFGLMLLAPWWLLT"
     gene            346..720
                     /locus_tag="AC16YU_07885"
     CDS             346..720
                     /locus_tag="AC16YU_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006085487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6065182.1"
                     /translation="MARVLGVGGVFFKSRDPRALGQWYREWLGIPVDPEWGGFGFPPG
                     DVPEHGLTVWSPFDSTTSYFDPSVQPFMVNLMVDDLDGALAQVARGGAEIVGNVEEFE
                     YGRFGWFLDPDGNKVELWQPAG"
     gene            801..2477
                     /gene="ettA"
                     /locus_tag="AC16YU_07890"
     CDS             801..2477
                     /gene="ettA"
                     /locus_tag="AC16YU_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6065183.1"
                     /translation="MSDQKIIFSMLGVGKIWKSTNRQVLRDIYLSFFHGAKIGVLGLN
                     GSGKSTLLRIIAGVDTDYLGKIESSKGVTFGYLPQEPELDPDRTVMEVVSEGAAEAVG
                     LLAEYNAVSEAFADPDADFDALMAKQAKLQDRLDHLGAWDIESKLELAMDALRCPPGD
                     MKIGPLSGGEKRRVALCRLLLQEPDVLLLDEPTNHLDAESVAWLEQHLARYTGTVIAV
                     THDRYFLDNVAGWILELDRGYGIPFEGNYSSWLEQKQARLAVEEKQASRRQKALEREL
                     EWVRLSPKGRHAKSKARISAYERMLEEQQERRDESVEIPIPPGPRLGRTVIECEGVSK
                     GYGDRLLFEDMTFSLPPGGIIGVIGANGAGKTTLFRMITGQETEDAGTIRIGDTVKLG
                     YVDQNRPLNPAKSVWEEISGGADTMKVGGRDVNSRAYVGLFNFGGSDQQKKTTELSGG
                     ERNRVHLAKVLKEGANVLLLDEPTNDLDVNTIRALEDALERFAGCAVVISHDRWFLDR
                     VATHILAFEGESRVAWFDGNYSEYEENRRERLGADADQPHRIRYKPLKRG"
BASE COUNT          438 a          887 c          925 g          400 t
ORIGIN      
        1 gtcgctcctg gtctggagtt tcttccggaa cgactaccgg ctgggcacgc aggtctcgct
       61 gttctggctc gcgcacaact gcatcaacat cagcgtgtac gcggcggacg cgcgcctgcg
      121 gcgcctcccg ctcctcgggg gagaccgcag cgagcacgac tggtggaacc tgctgggcat
      181 gaccgggctg ctcgcgtacg accaggtgtt cgggtggctg ttcttcgccc tggcgatcgt
      241 gctcttcggc ctcatgctcc tcgccccgtg gtggttgctc acgtgacccc ttgatttccc
      301 gacccggctg gccgatcgtt ttcccgaacc cagaccggac acatcatggc acgggtactc
      361 ggcgtcggcg gcgtgttctt caagtccagg gatcccaggg ccctcggcca gtggtaccgg
      421 gaatggctgg ggatcccggt cgatccggag tggggaggct tcggattccc gcccggggac
      481 gtgcccgagc acggcctgac cgtctggagc ccattcgact ccaccacctc gtacttcgac
      541 ccctcggtgc agccgttcat ggtcaacctg atggtggacg acctggacgg ggcgctggcg
      601 caggtcgccc gcggcggcgc ggaaatcgtg ggcaacgtcg aggagttcga gtacggccgc
      661 ttcgggtggt tcctggaccc ggacggcaac aaggtggagc tctggcagcc tgccggctga
      721 ggacgattcg gggcggtcgc agaaccttcg gtcgccgggg cgattcatcc gccgatccgc
      781 ccgcgtcatc cctcagcccc atgagcgacc agaagatcat cttttccatg ctcggggtcg
      841 gcaagatctg gaagtcgacc aaccggcagg tgctccgtga catctatctc tcgttcttcc
      901 acggcgcgaa gatcggcgtg ctgggcctga acggatccgg caagtcgacg ctcctgcgca
      961 tcatcgccgg ggtggacacg gactacctcg ggaagatcga atcgtcgaag ggcgtgacct
     1021 tcggctacct gccgcaggag cccgaactcg atccggaccg gacggtcatg gaggtcgtgt
     1081 cggagggcgc ggccgaggcg gtgggcctgc tcgccgaata caacgccgtc tcggaggcct
     1141 tcgccgatcc cgacgccgac ttcgacgccc tcatggcgaa gcaggcgaag ctgcaggatc
     1201 gcctggacca cctgggcgcc tgggacatcg agtcgaagct cgagctggcg atggacgcgc
     1261 tccgctgccc gcccggcgac atgaagatcg ggccgctgtc gggtggagag aagcgccgcg
     1321 tggccctgtg ccggctgctg ctccaggagc ccgacgtgct gctgctcgac gagccgacca
     1381 accatctcga cgccgagagc gtggcctggc tcgagcagca cctggcccgc tacacgggca
     1441 ccgtgatcgc cgtcacccac gaccggtact tcctggacaa cgtggcaggc tggatcctgg
     1501 agctggaccg gggttacggc atccccttcg agggcaacta ctcgtcctgg ctggagcaga
     1561 agcaggcccg cctggccgtg gaagagaagc aggcctccag gcggcagaag gccctcgaaa
     1621 gggagctcga gtgggtccgc ctgtcgccga agggccgcca cgcgaagagc aaggcccgca
     1681 tcagcgccta cgagcggatg ctcgaggagc agcaggagcg ccgggacgag tcggtcgaga
     1741 tcccgattcc cccgggcccg cgcctgggcc gcacggtcat cgagtgcgag ggcgtctcga
     1801 aggggtacgg tgaccgcctg ctcttcgagg acatgacgtt ttcgctgccg cccggcggga
     1861 tcatcggcgt gatcggggcc aacggcgccg gcaagacgac gctcttccgc atgatcacgg
     1921 gacaggagac cgaggacgcg ggcacgatcc ggatcgggga cacggtcaag ttgggctacg
     1981 tggaccagaa ccggccgctg aaccccgcga aaagcgtgtg ggaggagatc tcgggcggcg
     2041 cggacaccat gaaggtcggc ggccgcgacg tgaactcgcg cgcatacgtg ggtctcttca
     2101 acttcggcgg cagcgaccag cagaagaaga cgaccgagct gtcgggcggc gagcgcaacc
     2161 gcgtgcacct tgccaaggtg ctcaaggagg gagccaacgt gctgctcctc gacgagccca
     2221 cgaacgacct ggacgtgaac accatccggg cgctcgagga cgcgctggag cggttcgccg
     2281 ggtgcgccgt ggtcatctct cacgaccggt ggttcctgga ccgcgtggcg acccacatcc
     2341 tggcgttcga gggagagagc cgcgtcgcgt ggttcgacgg caactactcc gagtacgagg
     2401 agaaccgccg cgagcggctc ggcgcggacg cggaccagcc ccaccggatc cgctacaagc
     2461 cgctcaagcg cggctgacgc cgggcggcgc atcgcaggcc ccggggccgc gggcggccgg
     2521 acccggagcg ctccggcggc gacccgtccc gcggccgacg ctcgcagatc cggccggcgc
     2581 ccgatatctt cggccgccgg cgaaccatcc aatgcagggc accccgatgg acaggaaacc
     2641 gcgtctcgcc
//
LOCUS       DBNHQZ010000293         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2796544, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000293 DBNHQZ010000000
VERSION     DBNHQZ010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2796544"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="AC16YU_07895"
     CDS             complement(<1..647)
                     /locus_tag="AC16YU_07895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000225 -
                     N-acetylglucosaminylphosphatidylinositol deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR6065184.1"
                     /translation="MTRHLLAAALLAALSTVPAGAQDRGLSVLLVTAHPDDDATFSAT
                     VWRIVRERGGSADLAVVTDGAGGYRFATLAEPLYGLKLTDPEVAGTAIPAIRRREVQA
                     GGAIVGIRNYHFIDQPDTGKFLDVDSVFTHLWDTDLVVRRLAEIIRAGDYDLVFTMLP
                     TPATHAHHKGSALLTLRAVALLPADRRPLVLASTVGEEPGGLDFEVLDGHPDSRIL"
     gene            772..2025
                     /locus_tag="AC16YU_07900"
     CDS             772..2025
                     /locus_tag="AC16YU_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6065185.1"
                     /translation="MTPSSDMVEAGQRLASDLRRIRELRGVSIETFLEKTKASPDIIT
                     AFERNALVDHPLYNRVYLRSFVRMYAEAVDIDPDAALAAAEQLLEGRYDGALATRYLE
                     GEPAAKGPDEAASEAEPSAPAALPVEPSAPDAPAEPPSPEAQAEPPPPAQPDDRAPDE
                     PLPPSVAAPDQPIPPKPVDAPPPVEPMPGEAETPAASPAAEAPETDAEPPGDEPPSPA
                     DELPAPAATVPKPVQAPSAAGPPPPPTSGPLPTPAPKGAWTGSLKWIIPGIIALVIAA
                     VLLFGRSEPEVRETPVVPAEPTPIEPLPPAPVDTTDSIQVRMPPRVALPDSISVWVVA
                     ERDTLNPIRIKTDDDIRRPYWIEIGDSTEFRVRDRIALERETGVIALSVAGYRLPLDR
                     FVPGEPIELDRERIQGMLDSLRMVQ"
     gene            2034..4136
                     /gene="ligA"
                     /locus_tag="AC16YU_07905"
     CDS             2034..4136
                     /gene="ligA"
                     /locus_tag="AC16YU_07905"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6065186.1"
                     /translation="MTDPARLLARTRALRSELAASDLARGDAERFAPELRSVLSALAH
                     RYYVLDDPIVSDAEYDELIGVLRRIESVHPDLRTDDSPTLRVGGEPLPGFVKVAHPEP
                     LLSLANAFDEEDLRAWYARCRRGLGLDESTAVALVCELKIDGLAVALTYRDGVLVRAA
                     TRGDGAVGEDITENVRTIGSVPLRIPATGADVHVPASVEVRGEVYLPLAAFAALNERL
                     ARAGEKTFANPRNAAAGGLRQLDSRITAQRPLRFFGYSLGPVDGSRPGSQHAALDWMR
                     SVGIPVNPNATRVEGVDEAIEFCRRWAEERDALDYEIDGVVVKVDDLEQQRRLGAVTN
                     APRWAVAFKFPAREATTRLLDIIVNVGRTGVIKPEAVLEPVGIGGVTVSQATLHNEDY
                     IAGRDIRIGDTVVVKRAGDVIPQVVRPVTAARTGAERTWAMPTTCPACGNPLERLEGE
                     ADWYCVASDCPAQFIRLVEHFTSRGAMDIEGFGSRLAVQLAEEGLVRRLDDIYRLDAA
                     RLETLEGFAGKKARNLVDAIDSSRGRPLGRLLVGLGIRHVGKTIAEILARHHESVDAL
                     ASAAVEDLASIEGIGPVIAQSVVDWFAIEDNRNLVEGLRSVGVNLLRLAAEAPPATGG
                     ALEGRTVVVTGTLEGMSRKEAEERIKRAGGKVAGSVSNRTDYVVVGESPGSKADRARE
                     LGIPVLDQAAFMALVPEG"
     gene            4139..>4723
                     /locus_tag="AC16YU_07910"
     CDS             4139..>4723
                     /locus_tag="AC16YU_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR6065187.1"
                     /translation="MLTPLDYGVIAVYLVGVATLGIRLGGRQASATDYFLGGGRLPWW
                     AVSLSVVATETSTLTVIGIPAVAYGGSLTFFQLTIGYLLGRAVVAWLLLPRYFTGDLE
                     TAYGFLGARYGDGLRSLASVTFLATRLLADGVRLFATAIPIRVIMASAGFDVGYPAII
                     LAIALATIAYTYVGGLRAVVWMDVIQLALYVAGAI"
BASE COUNT          693 a         1615 c         1709 g          706 t
ORIGIN      
        1 aggatgcgcg agtccggatg gccgtccagg acctcgaaat cgagtccgcc aggttcctcg
       61 cccacggtgg aagccaggac gagcggcctg cggtcggccg gaagtagggc gaccgcccgg
      121 agcgtgagca gcgccgagcc cttgtggtgg gcgtgcgtgg ccggagtcgg cagcatggtg
      181 aataccaggt cgtagtcgcc cgcccggatg atctcggcca gtcgcctcac gaccaggtcg
      241 gtgtcccaca ggtgggtgaa cacggagtcg acatcgagga acttgcccgt gtcgggctgg
      301 tcgatgaagt ggtagttccg gatgccgacg atcgccccgc ccgcctggac ctcccggcgg
      361 cggatggccg ggatggccgt ccctgccacc tcgggatcgg tgagtttcag cccgtacagg
      421 ggttcggcga gcgtggcgaa gcggtagccc ccggcgccgt ccgtcaccac ggcgaggtcc
      481 gccgagccgc cccgctcgcg cacgatgcgc cacacggtgg ccgagaaggt ggcgtcgtcg
      541 tccggatggg ccgtgacgag gaggacggaa aggccgcggt cctgcgcgcc tgccggaacg
      601 gtggaaaggg ctgcaaggag cgcggccgca aggagatgcc gggtcatggt ggcggggtgc
      661 tgtcgttccg cccgatgggc gtccgtgatt cgctatacgg gccgcaccga tgcacggttt
      721 ggctcgcgcg ggtccgacgc cgtagtttcg tcccggtttc gagcacacga catgacccca
      781 tcgtccgaca tggtcgaagc cggccaacgc ctcgcttccg acctgcgcag gattcgtgag
      841 ttgaggggcg tctcgatcga gacttttctc gagaagacga aggcctcgcc cgacatcatc
      901 acggcattcg agcggaatgc cctggtggac cacccgctgt acaaccgggt gtacctgcgg
      961 tcgttcgttc ggatgtacgc ggaggcggtg gacatcgatc cggacgcggc cctcgcggcc
     1021 gccgagcagc ttctggaggg gcgatacgac ggcgccctgg cgacgcggta cctggagggc
     1081 gagcctgcgg cgaagggacc ggatgaggcc gcctcggaag cagagccttc ggcgccggcg
     1141 gcgctgccgg tggagccttc cgcgcccgat gcccccgccg aaccgccatc gcccgaagcc
     1201 caggccgagc caccgccgcc ggcgcaaccg gacgaccgcg cgcccgatga gccgctgccg
     1261 ccgtccgttg ccgcgccgga ccagccgatt ccgccgaaac cggtcgatgc tcctcctccc
     1321 gtcgaaccca tgccgggcga ggccgagacg ccggcggctt cgccggcagc cgaggcgccg
     1381 gagaccgatg ccgagccacc gggggatgag cccccgtcgc cggccgacga gctgcccgcg
     1441 cccgccgcga cggtgccgaa gcccgtacag gctcccagcg ctgccggccc accaccaccg
     1501 ccgacgtccg ggcccctgcc cacgccggcg ccgaagggcg cttggacggg gagcctgaag
     1561 tggatcatcc ccggcatcat cgcgctagtc atcgcggcgg tcctgctctt cgggcgatcg
     1621 gagcccgaag tgcgcgaaac cccggtcgtt ccggcggagc cgaccccgat cgagccgctt
     1681 ccacccgcgc cggtagacac gacggacagc atccaggtcc ggatgccccc ccgcgtggcg
     1741 ctccccgatt cgatctcggt ctgggtcgtg gcggaacggg acacgctcaa tcccatccgc
     1801 atcaagaccg atgacgacat ccggcggccg tactggatcg aaatcgggga ttcgaccgaa
     1861 ttccgggtaa gggaccggat cgcgctcgag cgcgagaccg gggtcattgc cctgagcgtg
     1921 gccggctacc gcctgccgct ggaccggttc gtaccgggag agccgatcga gctggaccgc
     1981 gagcggatcc agggcatgct ggattcgctg cgcatggtgc agtagctccg cccatgacgg
     2041 accccgcgcg tctcctcgcc cgcacgaggg cgctccgctc ggagctcgcg gcgtcggacc
     2101 tggcacgggg agatgcggag cggttcgccc ccgagctccg ctccgtcctc tcggcgcttg
     2161 cacacaggta ctacgtactg gacgatccga tcgtctccga cgcagaatac gacgagctga
     2221 tcggcgtgct tcggcgcatc gagtccgttc atcccgacct gcgcaccgac gactcgccca
     2281 ccctgcgcgt ggggggggag ccgcttccgg gattcgtgaa ggtggcgcac ccggagccgc
     2341 tgctctccct ggcgaacgcg ttcgacgagg aggacctccg ggcgtggtac gcccgctgcc
     2401 gccgcggcct gggcctggac gaatcgacag ccgtggcgct cgtctgcgag ctcaagatcg
     2461 acggcctggc cgtggcgctc acgtaccggg acggcgttct cgtgcgggcg gccacgcggg
     2521 gcgacggagc ggtcggggag gacatcactg agaatgtccg caccatcgga tcggtgccgc
     2581 tgcgcatacc ggcgacgggt gccgacgtcc acgtcccggc ctccgtcgag gttcggggag
     2641 aagtgtacct gccgctcgcc gccttcgctg cgctgaacga gcggctggcg cgggcgggcg
     2701 aaaagacgtt cgcgaatccg cgcaacgcgg ccgccggcgg cctgaggcag ctggactccc
     2761 gaatcacggc gcagcgcccc ctgcgcttct tcggctacag cctgggcccg gtggacgggt
     2821 cgaggcccgg ctcgcagcac gccgcactcg actggatgcg cagtgtcgga atcccggtca
     2881 acccgaatgc gacgcgcgtc gagggggtcg atgaggcgat cgagttctgc cgccgctggg
     2941 cggaagagcg cgacgcgctc gactacgaga tcgacggagt ggtcgtgaag gtggacgacc
     3001 tcgagcagca gcgcaggctc ggcgccgtga cgaacgcccc gcgctgggcc gtcgcgttca
     3061 agtttccggc ccgggaggcg accacgcggc tgctcgacat catcgtgaac gtggggcgga
     3121 cgggcgtgat caagccggag gccgttctcg agcccgtggg catcggcggc gtgaccgtgt
     3181 cgcaggccac cctgcacaac gaggactaca ttgccggccg ggacatccgg atcggcgaca
     3241 ccgtcgtggt gaagcgggcg ggcgacgtga tcccgcaggt ggtgcggccc gtcacggccg
     3301 ctcgcacggg tgccgagcgg acatgggcca tgccgacgac gtgtccggcc tgcggcaacc
     3361 cgctcgagcg gctcgaaggc gaggccgact ggtactgcgt ggcgtccgac tgcccggccc
     3421 agttcatccg cctcgtggag cacttcacct cgcggggggc gatggacatc gagggcttcg
     3481 ggtcccgact tgccgtccag ctcgccgagg aaggcctcgt gcgccggctg gacgacatct
     3541 accgtctgga tgccgcgagg ctcgagacgc tggagggctt cgccggaaag aaggcccgca
     3601 acctggtgga cgcgatcgac tcgtcgcgcg gccgcccgct cggccgactg ctcgtcggcc
     3661 tcggcatacg gcacgtgggg aagaccatcg ccgagatcct ggcccggcac cacgaatcgg
     3721 tcgacgcact ggcctctgcc gccgtggagg acctggcgtc catcgagggc atcggacccg
     3781 tgatcgcgca gagcgtcgtg gactggttcg cgatcgagga caaccggaat ctcgtcgagg
     3841 gtcttcgctc ggtcggcgtg aacctcctcc ggctcgccgc ggaggcgcct ccggccacgg
     3901 gcggcgcgct cgagggcagg acggtcgtcg tcacgggaac gctcgagggg atgtcgcgca
     3961 aggaggcgga ggagcggatc aagcgggcag gaggcaaggt ggccggttcg gtctcgaata
     4021 ggacggatta cgtggtcgtg ggcgagagcc cgggctccaa ggcggaccgg gcgagggagc
     4081 tcggcattcc cgtcctggac caggcggcct tcatggcact ggtcccggag ggttgagcat
     4141 gctgaccccg ctcgattacg gcgtcatcgc ggtgtacctg gtgggcgtgg ccacgctcgg
     4201 catacgcctg ggcggccggc aggcctcggc caccgactac ttcctgggcg gagggcgcct
     4261 accctggtgg gccgtttcgc tgtccgtggt tgccacggag acctccacgc tgaccgtcat
     4321 cggcatcccg gcggtcgcgt acggcgggtc gctcaccttc ttccagctca cgatcggcta
     4381 cctgctcggc cgcgcagtcg tggcgtggct tctcctgccc cgatacttca cgggcgacct
     4441 cgagacggcg tacggatttc tgggcgcgcg ctacggcgac ggactccggt cgctcgcgtc
     4501 ggtcacgttc ctggccacgc ggctgctggc ggacggggtt cggcttttcg cgaccgcgat
     4561 tcccatccgc gtgatcatgg catccgccgg gttcgacgtc gggtacccgg cgatcattct
     4621 cgcgatcgcg ctcgcgacga tcgcgtacac ctacgtcggg ggccttcgag ctgtggtctg
     4681 gatggacgtg atccagcttg cgctgtacgt cgcgggggca atc
//
LOCUS       DBNHQZ010000294         2397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3340996, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000294 DBNHQZ010000000
VERSION     DBNHQZ010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3340996"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..700
                     /locus_tag="AC16YU_07915"
     CDS             149..700
                     /locus_tag="AC16YU_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065188.1"
                     /translation="MRISVFIVCLVLLTLYATPAEAQLRQDAAVANAPVRLYDQGGPG
                     FSLNRFFSPEHFRMSHSVELSSSSFGGYGGTLAMYTNSMMWQFSSKLAARVDLAMAYS
                     PTSSYPGMSATGQGDTRVFLRNAELAFRPKENMLLHLSVRQSPYGMYASPYGWAGGYG
                     PSYGFDAWDQGTRGDLFWNSRLR"
     gene            779..1219
                     /locus_tag="AC16YU_07920"
     CDS             779..1219
                     /locus_tag="AC16YU_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR6065189.1"
                     /translation="MVTDRFGNLGLNAAILLLGLVMVLLGWSLVGGWFGGDPRAADDA
                     ASHEIVQLEVRNGCGTSGLAARAAEWLRGEGVDVLEAGDWTRFDVRETYVIDRIGDPV
                     PAGEVLRLLELDPDRIREDVRSDYYLDATLVIGCDYESLPPFRE"
     gene            1265..1729
                     /gene="rsfS"
                     /locus_tag="AC16YU_07925"
     CDS             1265..1729
                     /gene="rsfS"
                     /locus_tag="AC16YU_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6065190.1"
                     /translation="MKIIEEALKREGRAKAKRTTGIPARVLAREAVDALLGKKVHDIL
                     VMDMRGVSGVADLFVLATGDSDLQIKAAVDAVRESLKERFGERPWHVEGYEHRQWVLV
                     DYVDLVVHVFTDERRAFYGLERLWGDAPREAVPDEGSGADVRILADPTEKGT"
     gene            1726..2229
                     /locus_tag="AC16YU_07930"
     CDS             1726..2229
                     /locus_tag="AC16YU_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065191.1"
                     /translation="MTRLRETSILLAVLALGAGCQAARQGSRPPDEQFGHRVEGTDQE
                     GRETVVVVPPSGDQEYLTYPAVYESVTVRPGTAEPGRTDLAVEVLVKGAFPDACTELH
                     EVSQQRSGNLVDVSLSMRRPQGAICATVLRPYRFYVLLDGRFEPGPYNLTLNGDSHPF
                     ELREPGS"
BASE COUNT          366 a          799 c          844 g          388 t
ORIGIN      
        1 ccgtcggccc ttcgccgtcg gatggcgcgg aaaatgccct cttcaccatg attccccgta
       61 gaacaaacct cggggggcct cgttcttgac acaggaccga ccccccgtct ataatacaac
      121 atcgcccgtg caagccggag ggaagcgtat gcgcatatcc gtgttcatcg tctgcctcgt
      181 actgctgacc ctgtacgcca cgcccgcgga ggcccagctg cgccaggatg cggcggtcgc
      241 gaacgcgccg gtgcggctct acgaccaggg cggcccgggg ttttcgctca accgcttctt
      301 cagcccggag catttccgga tgagccactc ggtggaactg tcctcgagct cgttcggggg
      361 atacggcggg acgctggcga tgtacacgaa ctccatgatg tggcagttct cgagcaagct
      421 ggcggcccgg gtggacctgg cgatggccta ttcgcccacc tcctcctatc cgggcatgag
      481 cgcgacgggg cagggtgaca cgcgggtctt cctgcgcaac gccgaactcg cattccggcc
      541 gaaggagaac atgctcctcc acctctcggt gcgccagagc ccttacggga tgtacgcctc
      601 gccgtacggc tgggccgggg gctacggccc gtcctacggg ttcgacgcct gggaccaggg
      661 aacgaggggc gaccttttct ggaactcccg ccttcgctga cgggagcggt ccgggccgtc
      721 gttcatggcg gcatgtcctg gcagaccccg cgagacaccg tccacctcct gataccggat
      781 ggtcacggat cgcttcggaa acctgggtct gaatgcggca atcctgctgc tggggctggt
      841 catggtgctg ctgggctggt cgctcgtggg cggctggttc ggcggggacc ctcgcgcggc
      901 ggacgacgcg gcgagccacg agatcgtcca gctcgaggtg cgcaacggct gcggcacgtc
      961 cggccttgcc gcccgcgccg cggaatggct gcggggggag ggcgtggacg tgctggaagc
     1021 gggcgactgg acgcgcttcg acgtgcggga gacctacgtc atagaccgca tcggcgaccc
     1081 cgttccggcc ggggaggtgc tccggctgct cgagctcgat ccggaccgca ttcgcgaaga
     1141 cgttcgatcg gactactatc ttgacgccac gctggtcatc ggctgcgatt acgagtcgct
     1201 ccccccgttc cgggagtgat ccggggcacc cggcgccggc catctctcat tcaaccacag
     1261 ctagatgaag atcatcgagg aagcgctcaa gcgcgagggc agggcgaagg cgaagcgcac
     1321 gaccggcatc cccgcacgcg tgctggcacg cgaggcggtc gacgcgctgc tgggcaaaaa
     1381 ggtgcacgac atcctcgtca tggacatgcg gggcgtgagc ggggtggccg acctcttcgt
     1441 tctcgccacg ggggattccg atctgcagat caaggccgcc gtggacgcgg tccgcgagag
     1501 cctgaaggag cggttcgggg agcgaccctg gcacgtggag gggtacgagc accggcagtg
     1561 ggtcctggtc gactacgtcg atctcgtggt ccatgtgttc accgacgagc gccgcgcctt
     1621 ctacggtctg gagcgcctgt ggggcgacgc cccgcgggag gccgtgccgg acgagggcag
     1681 cggcgcagat gtgcggatcc tcgcggatcc cacggagaag ggcacatgac gaggctccgc
     1741 gagacatcga tcctcctggc cgttctcgcg cttggcgcgg gatgccaggc cgcccggcag
     1801 ggctcgcgcc cgccggacga gcagttcggc caccgcgtcg agggcacgga ccaggagggt
     1861 cgtgagacgg tcgtggtcgt tccgccgtcc ggggaccagg agtacctcac ctacccggcg
     1921 gtctacgagt ccgtcaccgt gcggccgggg acggccgagc ccggccgtac ggacctggcc
     1981 gtcgaggtcc tggtcaaggg agcgtttccc gatgcctgca ccgagctgca cgaagtgtcc
     2041 cagcagcgct ccggaaacct ggtcgacgtg agcctgtcga tgcgccgccc gcagggtgcg
     2101 atctgcgcca ccgtgctgcg gccctaccgc ttctacgtgc tgctcgacgg ccgcttcgag
     2161 ccgggtccgt acaacctgac gctgaacggc gacagccatc cgttcgagtt gcgcgagccc
     2221 ggttcctgag ggccccgacg tcaggggagc agggtgtcgg gcgcgacgag accggcctcg
     2281 acgtcccgca tgacggcctc gtactgggcg cgcagctcct cggcgctctg ggcgtagctc
     2341 gagtccccga gcacgtccgc gatgcgctcc gtgaaggcgg ccgcgaggtc gaactcg
//
LOCUS       DBNHQZ010000295         3962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3067453, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000295 DBNHQZ010000000
VERSION     DBNHQZ010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3067453"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3926)
                     /locus_tag="AC16YU_07935"
     CDS             complement(<1..3926)
                     /locus_tag="AC16YU_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065192.1"
                     /translation="MQGGRIDLDRPVWRARYGGSPGALRVPAGLAASGLADRVGDVFG
                     GPIPASELHVEHDLADGPVRHLRYAQVVGGVPVLDRTVSVALDRDGRVLFGLNGFDYD
                     VSARPPETRPAIGAGEARQRARQELDGRGGKIGPATLVVVPGEHPRLAWRLYAWPEGP
                     PSEWRVLVDAQTGDVIGLQDETVRKSGTAPEASTDHGLPEPGLPEIATSSPADVTQDG
                     RGAVFDPDPLATSGEAYIFPYTDQDDQDIAELNQERRPITLRDITLGNDGLYRLDGPY
                     VTIVGVNSAGTRVYDPPKEPTSDGFSYTRAKDGFEAVSAYHHLDLSQRHIQALGFTDL
                     QANPLPVNPAGTTADESGWIASLRYLFFGTGGVDDGEDAFVLWHEYAHAILEAGAPGL
                     RATAEGRALHEGWSDYWAASYQRHLVETGKMTRTDWEVAFRWDAGDGTIWAGRTLDHP
                     GTYPASTCSDDPNPGACSPHNDGRLWATVLMEIHQELGRDLTDRLNLLSHRYLSAPVT
                     FRDAAEAVVQADIDYYGGEHLDVIVGRFAPRGLVDMADFWPTARHEPLPSTEETGSAI
                     TIDVEAIGYSAPIEEVAVVYRLGNGAAETLALQPVGGNVWRGAFTGPATPDSVFYWIR
                     VTDAVDRTVLLPDEAPAAEFSFYVGPDERSPAIEHEPIDLASIAAWPPVVSARITDGL
                     GVAAASVEYSIVGPDGLPVDSGTFDLAPGEDDVWTAPFPVAVGSVRHGTLVRYALRAT
                     DASAAGNETRSPASGYHEFSVAGTGVLRRFAADSGQAGVTLSGSWEAGEPRYGVFASP
                     LGEAVWATASEGATPATATLSRLRLHALDLRGQEAVYLVFRHWYDTEHDGSAEPGKDA
                     GAVLWDGGNVKLSTDGGSTWTPAAPEGGWSGSVRSGEDNPLAGESAFGGYSYAWRQEI
                     VPLPTVAGVEIAFDFGTDATNTAESIGFAGWYLDDVRITTERPSDATAPFVTDAPAGR
                     TVWPAGTAKPLVSIRVADDVGVADVRVEYAWTRGSEVHRDTLRVPMSPYDVALFETTL
                     DVGLDPRPDDHIELRFHIEDVSGNRIEWPEGAGDWIVIDVMSLDVADMMATARASGGW
                     TSGIEGWTVQDSTGSLVLAPHWLAGNDRSAVLDIVHHFAFGPGRGGRAEVSRDDGATW
                     SPLVPEAGYPSTLDGKGVWDGQPIGHRTDRFDLGNLAGAAVQVRFSVVAVGSWSPMSW
                     TIESAELVQQTGDDTFDLPDAPILHANFPDPFAGSTNISVTLPESGPVSLAVYDVLGR
                     RVAVLMDGVPAAGTHNLTFDASALTPGVYLLRLDAGGVILTERMVRA"
BASE COUNT          587 a         1451 c         1365 g          559 t
ORIGIN      
        1 gccctgacca tgcgctcggt gaggatcact cccccggcgt cgaggcgcag aaggtacacg
       61 cccggcgtca aagccgacgc atcgaacgtg aggttgtgag ttccggcagc gggcaccccg
      121 tccatcagga cggcgacgcg acgcccgagc acgtcgtaga cggccagcga caccgggccg
      181 gactcgggca gggtgacgct gatattcgtc gatccggcaa agggatccgg aaagttcgcg
      241 tgaaggatcg gggcgtcggg caggtcgaac gtgtcgtccc cggtctgctg cacgagctcc
      301 gcgctctcga tggtccacga catcggcgac cacgagccga ccgcgacgac ggagaaccgg
      361 acctgcacgg ccgctcccgc gaggttcccc aggtcgaagc ggtccgtgcg gtgaccgatc
      421 ggctggccgt cccagacgcc cttgccgtcg agcgtggacg gatacccagc ctcggggacc
      481 aggggactcc aggtggcgcc gtcgtcccgc gaaacctcgg cacgcccccc tcggcccgga
      541 ccgaaagcga aatgatgcac gatgtcgagg acggcgctcc ggtcgtttcc ggccagccag
      601 tgcggggcca ggaccaggga tcccgtcgaa tcctgcacgg tccagccctc gataccgctc
      661 gtccaacccc cgctggcgcg ggcggtggcc atcatgtcgg ccacatccag gctcattacg
      721 tcgatcacga tccagtcccc ggcgccttcc ggccactcga tccggttgcc cgacacgtcc
      781 tcgatgtgga agcggagctc tatgtggtcg tccggacggg gatcgaggcc cacgtccagc
      841 gtcgtctcga aaagcgccac gtcgtagggc gacatcggca cgcggagggt atcccggtgg
      901 acttcggacc cccgggtcca ggcgtattcg acccgtacgt cggcgacgcc cacgtcgtcc
      961 gcgacccgga tcgacacgag cggcttcgcg gtcccggccg gccacacggt ccggccggcc
     1021 ggggcgtccg tcacgaacgg tgccgtggca tccgagggcc gctccgtcgt gatccggacg
     1081 tcgtcgaggt accaccccgc gaaaccgatc gactcggcgg tgttcgtagc gtccgtgccg
     1141 aaatcgaacg cgatctccac gcccgccacc gtgggcagcg gcacgatctc ctgccgccag
     1201 gcatagctgt atccgccgaa cgcggactcg ccggccaggg gattgtcctc gcccgaccgg
     1261 acgcttccgg accacccccc ttcgggcgcg gccggcgtcc acgtcgatcc gccgtcggtg
     1321 gacagcttga cgtttccgcc gtcccagagc accgcccccg cgtcctttcc gggctcggcg
     1381 ctgccgtcat gctccgtgtc gtaccagtgg cggaagacga ggtacacggc ctcctggccc
     1441 cgcaggtcca gcgcgtgcag gcgcagccgg gaaagcgtcg cggtcgcggg cgtcgcgccc
     1501 tcggacgccg tggcccagac cgcctccccg agcggcgagg cgaacacgcc gtaccggggc
     1561 tctcccgcct cccaggagcc cgaaagcgtc actccggcct ggccggaatc ggccgcgaac
     1621 cggcgcagca cgcccgtccc cgccacgctg aattcgtgat accccgacgc ggggctgcgc
     1681 gtctcgttgc cagccgcgga cgcatccgtc gcgcgcaggg cgtaacgcac aagggtgccg
     1741 tgccgcaccg aacccaccgc gaccgggaat ggggccgtcc acacgtcatc ctcgcccggc
     1801 gcgaggtcga acgttcccga gtcgaccgga aggccgtccg gaccgacgat ggaatactcg
     1861 acgctcgccg ccgccactcc gagcccgtcc gtgatccgtg ccgagacgac gggaggccaa
     1921 gccgcgatgg aggcaaggtc gatcggctcg tgctcgatgg ccggcgagcg ctcgtcaggg
     1981 cccacgtaga acgagaactc ggcagccggc gcctcgtccg ggagaaggac cgtgcgatcc
     2041 acggcatccg tcacccggat ccagtagaac acggaatcgg gcgtcgccgg acccgtgaac
     2101 gcgccgcgcc atacgttgcc gccgaccggt tgcagggcga gcgtctcggc cgccccgttt
     2161 ccgagccggt acacgaccgc gacctcctcg atgggagccg agtacccgat cgcctcgacg
     2221 tcgatcgtga tggccgagcc cgtctcctct gtcgaaggca gcggctcgtg gcgcgccgtc
     2281 ggccagaaat cggccatgtc caccaggccc cggggggcga accggccgac gatcacgtcc
     2341 aggtgctcgc caccgtagta gtcgatgtcg gcctggacca cggcctccgc ggcatcccgg
     2401 aaggtgaccg gggccgagag gtagcgatgc gagagcaggt tcaggcggtc ggtgaggtcg
     2461 cgtccgagct cctggtggat ctccatgagg acggtcgccc acagccggcc gtcgttgtgc
     2521 gggctgcacg cgccggggtt gggatcgtcg gagcacgtcg acgcggggta ggtgcccggg
     2581 tggtccaggg tccggccggc ccagatggtg ccgtctcccg cgtcccagcg gaacgcgacc
     2641 tcccagtccg tccgggtcat cttgcccgtc tcgaccaggt gccgctggta ggatgccgcc
     2701 cagtagtcgg accagccctc gtgcagcgcg cgcccctccg cggtcgcccg aaggccgggt
     2761 gccccggcct ccaggatggc gtgcgcgtac tcgtgccaca ggacgaaggc gtcctctccg
     2821 tcgtccacgc cgcccgtgcc gaaaaagaga tagcgcaggc tcgcgatcca gccactctcg
     2881 tccgccgtcg tcccggcggg gttcacggga agggggttgg cctggaggtc cgtgaagccg
     2941 agcgcctgga tatggcgctg ggaaagatcc aggtgatggt aggcgctcac ggcctcgaat
     3001 ccgtctttgg cccgcgtgta ggagaacccg tccgaggtgg gctccttcgg cgggtcgtat
     3061 accctggtcc cggccgaatt cactccgacg atcgtcacgt agggaccgtc gaggcggtac
     3121 agcccgtcgt tccccagggt gatgtcccgg agcgtgatcg ggcggcgctc ctggttgagc
     3181 tcggcgatgt cctggtcgtc ctggtccgtg tagggaaaga tgtaggcctc cccggaggtg
     3241 gcgagcgggt ccggatcgaa cacggccccg cgaccatcct gggtcacgtc cgccggggac
     3301 gacgtcgcaa tctccggcag gcccggctcg ggcaacccgt ggtccgtcga ggcctccggc
     3361 gcggtccccg acttccggac cgtctcgtcc tgcaggccga tcacgtcgcc cgtctgcgcg
     3421 tccacgagca ccctccactc ggagggcggg ccctcgggcc aggcgtacag gcgccaggcc
     3481 agccgcggat gctcgcccgg cacgacgacc agcgtcgcgg gtccgatctt tccgccccgt
     3541 ccgtcgagtt cctgccgtgc ccgctgccgg gcttcccctg ctccgatcgc cggcctcgtc
     3601 tccggcggac gcgccgacac gtcgtagtcg aatccgttca ggccgaaaag cacccggccg
     3661 tcccggtcga gcgccacgga caccgtccgg tccaggaccg gcacgccgcc gacgacctgg
     3721 gcatagcgga ggtgcctcac gggcccgtcc gcgaggtcat gctccacgtg gagctcggac
     3781 gccggaatcg gtccgccgaa cacgtcgccc acgcggtcgg ccaggcccga cgccgccagg
     3841 ccggcgggaa cgcgaagcgc cccgggcgag ccgccgtagc gggcgcgcca caccggccgg
     3901 tccaggtcga tgcgtccgcc ttgcacccgc gcggtgcgga accgtgggcg ggcggcggaa
     3961 tc
//
LOCUS       DBNHQZ010000296         2900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_360489, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000296 DBNHQZ010000000
VERSION     DBNHQZ010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_360489"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..883)
                     /locus_tag="AC16YU_07940"
     CDS             complement(149..883)
                     /locus_tag="AC16YU_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR6065193.1"
                     /translation="MTRKLLTAFVAILLAAPAQAQEWPPELTEVWQPEPPVVTPAFGV
                     RPPSDAEILFDGTSLDAWERAADGGEAMWSLADGAMTVVSGSGDIRTRASFGDAQLHI
                     EWRTPAEVVGEGQGRGNSGVFLQDRYEVQVLDSFGNRTYSNGQAASVYKQHIPMVNAS
                     RGPGEWQTYDVFYRAPVFTAAGEVARPALVTVVHNGVLVLHAAEIRGNTEYRGLPAYE
                     PHGDLPIRLQDHGNPVSYRNIWIRRL"
     gene            complement(910..2250)
                     /locus_tag="AC16YU_07945"
     CDS             complement(910..2250)
                     /locus_tag="AC16YU_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065194.1"
                     /translation="MPLSRRRFVRTAAMSAVAFSIPGMPVRAARRRRASAPSDALRFG
                     VIGVAGMGFSNLRSFLRVDGVTCAALCDVDANVLERRAAETADLQGSRPDTFADHRAL
                     LERADLDFVVIATPDHWHPILTLDAVGAGKHVYVEKPLANSIGEADVMHAGIRRSDRL
                     VQVGQWQRSGPHWRDAVAFVRSGALGNIRTVKTWAYQGWMEPVPVRPDGPVPDGVDYD
                     RWLGPASSRPFNPNRFHFNFRWFWDYAGGLMTDWGVHILDFALFGMDAGWPRSVMASG
                     GKFAYPDDASETPDTLQAIYEFDGFTLLWDHATGIDLGPYGRTHGVAFVGNNGTLIVD
                     RGGWEVIAETEDDNRGGRRPKVEIPQPERYDGSDLDRHTLNFTAAIRTGEALNADISV
                     GHRVAAIAHMGNVAFRAGRKLHWDAEAHAFPGDDEAARLSAGNPYRAPWSLPTF"
     gene            2373..>2900
                     /locus_tag="AC16YU_07950"
     CDS             2373..>2900
                     /locus_tag="AC16YU_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6065195.1"
                     /translation="MHIASIELFRAEIPFHEPFRYALTESASARNLFVRITADTGQYG
                     WGEASPTRPITGDTQATVFEASVELGRLLLGADPRDIVGAVRAMRSLLAGNTTARSAF
                     DMALHDLVGKASGLPLHALLGGGARRFRTDNTIGIADPDVMASKARRFLEEGFTAIKV
                     KLGTGFEEDVARVVAI"
BASE COUNT          433 a          989 c         1053 g          425 t
ORIGIN      
        1 cttcccgggc agcgccacgg cttcggcaag atgaccgcgt acgccgaccg actgacgtgg
       61 tactacttcg ccgagcacct gctcggcgat taccgcacga acgtcgactt ccacatcccg
      121 gaggaggacg cggagtgagc cgcgggcctc agagcctgcg gatccagatg ttgcggtagc
      181 tcaccgggtt gccgtgatcc tgcagccgga tgggcaggtc gccgtgcggc tcgtaggccg
      241 gaagcccccg gtactcggtg ttgccccgga tctcggcggc gtggagcacc aggacgccgt
      301 tgtgcaccac cgtcacgagg gcgggacgcg ccacctcgcc ggcggccgtg aagacggggg
      361 cacggtagaa gacgtcgtac gtctgccatt cgcccgggcc gcgtgacgcg ttgaccatgg
      421 ggatgtgctg cttgtagacg gatgccgcct gcccgttcga atacgtccgg ttcccgaagg
      481 agtcgagcac ctgcacctcg tagcggtcct ggaggaagac cccggagttg ccgcgaccct
      541 ggccctcgcc gaccacctcg gcgggggtgc gccactcgat gtgcagctgc gcgtcgccaa
      601 aggaagcccg tgtccgtata tcgcccgaac ccgacacgac ggtcatggcc ccgtcggcga
      661 ggctccacat cgcctcgccc ccgtcggccg cccgctccca ggcgtcgagc gaggtgccgt
      721 cgaacaggat ctcggcatcc gacggcggcc ggactccgaa ggccggcgtg acgacgggcg
      781 gctccggctg ccacacctcc gtgagctcgg gaggccactc ctgtgcttgg gcgggcgcgg
      841 cgaggaggat cgcgacgaat gcggtaagca gcttgcgggt catggccgga aggctggaag
      901 gtctgggagt cagaaggtgg gaagggacca cggagcccgg tacggattcc cggccgagag
      961 ccgggcggcc tcgtcgtcgc cggggaacgc gtgggcctcg gcgtcccagt ggagctttcg
     1021 acccgcgcgg aatgccacgt ttcccatgtg ggcgatggcc gcgacccggt ggccgaccga
     1081 gatgtccgcg ttcagggcct ccccggtccg gatcgccgcg gtgaagttca gcgtgtggcg
     1141 gtccaggtcg cttccgtcgt agcgctctgg ctgcgggatc tccaccttgg gtcgacggcc
     1201 gccacggttg tcgtcctcgg tctcggcgat gacctcccag ccgccgcgat ccacgatgag
     1261 cgtgccgttg ttgccgacga acgccacgcc gtgggtgcgt ccgtacgggc ccaggtcgat
     1321 gccggtcgcg tggtcccaca gcagggtgaa cccgtcgaac tcgtagatgg cctgcagggt
     1381 gtcgggcgtc tcggaggcgt cgtcggggta ggcgaacttg ccgcccgacg ccatgaccga
     1441 ccgcggccag ccggcgtcca tgccgaagag cgcgaagtcc aggatgtgca cgccccaatc
     1501 ggtcatcagg cccccggcgt agtcccagaa ccagcggaag ttgaagtgga agcggttcgg
     1561 gttgaagggt ctcgatgagg ccggcccgag ccagcggtcg tagtcgaccc cgtcgggcac
     1621 ggggccgtcg ggccggacgg ggacgggctc catccatccc tggtaggccc aggtcttcac
     1681 ggtgcggatg ttgcccagcg cgcccgagcg cacgaacgcg acggcgtccc gccagtgcgg
     1741 tcccgagcgc tgccactggc ccacctggac cagccggtcg gaccgccgga tgcccgcgtg
     1801 catcacgtcg gcctcgccga tcgagttcgc gagcggcttc tccacgtaca catgcttccc
     1861 ggcacctacg gcatccagcg tgaggatggg gtgccagtgg tccggggtcg cgatgacgac
     1921 gaaatccagg tcggcgcgct cgaggagagc ccggtgatcg gcgaacgtgt cgggccgcga
     1981 tccctgcagg tcggccgtct cggcggcgcg gcgctccaga acgttcgcgt ccacgtcgca
     2041 gagcgcggcg caggtcacgc cgtcgacccg gagaaacgaa cggaggttgc tgaatcccat
     2101 gccggcgacg ccgatcacgc cgaagcgcag ggcgtccgac ggagcggagg cccggcggcg
     2161 ccgggcggcc cggacgggca tgccggggat gctgaaggcg acggcggaca tcgcggccgt
     2221 ccggacgaat cggcggcggg agaggggcat ggctcgcagg ggtcggtgct gtgtcgaacg
     2281 tacccctcgg ggcccgcagg ggcaagggcc ggggtagggc acgatccctc cgcttcatac
     2341 ctttcggccc cagggagcac ggggcatccg ccatgcacat cgcaagcatt gaactcttcc
     2401 gggccgagat tccgttccac gagccgttcc gctacgccct gaccgagtct gcctcggcgc
     2461 ggaacctctt cgtccgcatc acggcggata ccgggcagta cgggtggggc gaggccagcc
     2521 ccacgcggcc gatcacgggg gatacccagg ccaccgtttt cgaggcgtcg gtcgagctgg
     2581 gccggctgct cctcggggcc gatccgcggg acatcgtcgg ggccgtccgc gcaatgcgct
     2641 ccctcctggc gggcaacacg acggcccgca gcgccttcga catggcgctg cacgacctgg
     2701 tcggcaaggc gtcggggctt ccgctccacg ccctgctcgg cggcggagcc cgccggttcc
     2761 ggaccgacaa caccatcggc atcgcggacc ccgacgtgat ggcctcgaag gcccgccgct
     2821 tcctggagga gggattcacg gccatcaagg tcaagctcgg gaccggtttc gaggaggacg
     2881 tggcgcgggt cgtcgcgatc
//
LOCUS       DBNHQZ010000297         1940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1040541, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000297 DBNHQZ010000000
VERSION     DBNHQZ010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1040541"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC16YU_07955"
     CDS             <1..321
                     /locus_tag="AC16YU_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065196.1"
                     /translation="EARLRPGLGELYRWYRSSQDMLGNVLRDAALVPAVGEVLDARWW
                     PYVERLADVFEGAPSDGRDATDRRAAIRLVVDFRTWHALAGSGLDDVRAADLAARMVT
                     CAVG"
     gene            573..872
                     /locus_tag="AC16YU_07960"
     CDS             573..872
                     /locus_tag="AC16YU_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065197.1"
                     /translation="MYKLREVLHCRPGKVGELVKRFKELGKVMRDMNYEPFRLFTDVS
                     GEQFWTLVLESESATLADFQTMEDEVMSDERARAIMAGYHDFVLDGRREIFKVEA"
     gene            1001..1525
                     /locus_tag="AC16YU_07965"
     CDS             1001..1525
                     /locus_tag="AC16YU_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065198.1"
                     /translation="MCRFGLVVLAVLALGLPTARAQDTRPLEADPGSRYYDFWPGTWF
                     QVVNGAVDTTRTAFRVEVDVHRAAFVERWRLVIPPADTLHATALRAWDVTEDRWMYTW
                     ISDGGLHQVWDGRRVGNDWYLYREFVTPDGPVLSRQAWIPRGPGRLERISEHSVDGGE
                     TWQLRFREEYRRRN"
     gene            1550..>1940
                     /locus_tag="AC16YU_07970"
     CDS             1550..>1940
                     /locus_tag="AC16YU_07970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6065199.1"
                     /translation="MRLKLAILVLVSAGCGPQAPRPVSIPVPDGGTIHADEYGAGDRA
                     VVLAHGGRFDRSSWGAQARVLEKAGWRVVAIDFRASVLTRSGEETDCLYDASCLAVDV
                     LAAVRYVRESGAETVAVIGASLGGGAAA"
BASE COUNT          288 a          655 c          685 g          312 t
ORIGIN      
        1 gaagcgcggc tgcggccggg cctcggggaa ctctatcgct ggtaccgctc ctcgcaggac
       61 atgctgggga acgtgctgcg tgacgccgcc ctcgtgcccg ccgtcgggga ggtcctcgac
      121 gcccggtggt ggccgtatgt cgagcgactc gcggacgtgt tcgagggggc cccgtcggac
      181 ggtcgggatg ccaccgatcg acgggcggcg atccggctcg tcgtcgactt ccgcacgtgg
      241 catgcgctgg cggggtcggg cctcgacgac gttcgagcgg ccgacctcgc cgcccgcatg
      301 gtgacctgtg ccgtgggctg accggtcggc ggatcgactg ccgacacgcc tcccggggca
      361 ttctcggccc agcgcggcgg ggcggcggac agtcttcgcc cttgacgcgg cacgtcgatt
      421 cccctaccct atagagccgt tcgggaccgt ccctccgaaa ccccctcggc gttcggcccc
      481 gtcacctcct cgtggcgacc ggtgacgagt atcgcgcgcg gcgtcgcgag gcgaccagtc
      541 acaagacggc ggccctgccg cccccgagag ccatgtacaa gctgcgtgaa gtcctccatt
      601 gccggcccgg caaggtcggc gagctcgtca agcgtttcaa ggagttgggg aaagtgatgc
      661 gcgacatgaa ctacgagccg tttcgcctat tcaccgacgt cagcggcgag cagttctgga
      721 ccctggtcct ggaatccgaa tccgccacgc tggccgactt ccagaccatg gaagacgagg
      781 tcatgtcgga cgaacgggcc cgggcgatca tggcggggta ccacgatttc gtgctggatg
      841 gacgccgcga gatcttcaag gtggaagcgt gacgggcgtc gcccattggc cgatgcgatt
      901 cccggtcgcg tatcggcgcg tttgactccc gacctgaagg cacggcgccc ggaaggccgg
      961 gctcgccgcg ccggtcagcc acgtaacccc agtcgaacac atgtgccgat tcggccttgt
     1021 cgtcctcgcc gtccttgcgc tcggcctgcc cacagcccgc gcacaggaca cgcgcccact
     1081 cgaagctgat cccgggtcgc gctattacga tttctggcca ggcacgtggt tccaggtggt
     1141 gaacggtgcg gtcgacacga cccgcacggc gttccgcgtc gaggtggacg tgcaccgggc
     1201 cgcattcgtg gagcgatggc gcctcgtaat ccctcccgcc gacaccctgc acgccacggc
     1261 cctgcgcgcg tgggacgtga cggaagaccg ctggatgtac acgtggatca gtgacggcgg
     1321 actccaccag gtctgggacg ggcgccgcgt cggaaacgac tggtacctgt atcgcgagtt
     1381 cgtcacgccc gacggcccgg ttctttcccg ccaggcctgg attcctcgcg gacccggccg
     1441 cctggagcgg atcagcgagc actcggtgga cggcggcgag acctggcagc tccgattccg
     1501 ggaagagtac cggcgacgga actgatccga gaacaggagg cgaatatcat tgcgtctcaa
     1561 gctggcgatc ctggtgctgg tttccgccgg ctgcgggccg caggcgccgc gcccggtctc
     1621 catccccgtc cccgatgggg gcacgatcca tgccgacgag tacggcgccg gggaccgagc
     1681 cgtggtgctt gcgcacggag ggcgattcga ccggtcgagc tggggagccc aggcacgggt
     1741 gctagagaaa gccgggtggc gggtggtggc catcgacttc cgggcgtccg tcctgacgcg
     1801 atccggggaa gagaccgact gcctgtacga cgcgtcgtgc ctcgccgtcg acgtcctcgc
     1861 cgccgtccgg tatgtccgcg aatcgggcgc tgagacggtt gcggtaatcg gggccagcct
     1921 gggtggaggc gccgcggcgc
//
LOCUS       DBNHQZ010000298         7179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1716095, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000298 DBNHQZ010000000
VERSION     DBNHQZ010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1716095"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="AC16YU_07975"
     CDS             <1..1205
                     /locus_tag="AC16YU_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6065200.1"
                     /translation="SWVPVPSVNRYGRDTLQTRPYELAAYIQDKMEFRNLIVNAGIRF
                     DYFEPDRQIPVQWEEAGSIWVPVYDDNDVATGDSLYNREDGPARWQISPRIGIAFPIS
                     ATGVIRFSAGLFFQIPQLSILFTNPNYEVNPASANIQFGNPALDPERTLHFEIGLQQQ
                     LTDEMGLELTLFSKDIRNLTGVEIRRDVETTSTTVRYINRDVGTSRGLTLSLFQRPIG
                     GISWDLDYTLQFADGTSSNPTEAFGRFQSGQEEILTLVRLDWDRRHVLTNSITVTPAQ
                     AFSVTLINRYQTGAPYTTERQFVTSYIRNNADRPAGFTSDLRLFYRPWFVSGLSLTAQ
                     VDNLFDAMIHYGVYNDSGRGDETVSMELYRRSGTPIGGVNSLDEYFFNQGWFSVPRRV
                     TVGLRYDF"
     gene            1216..4713
                     /locus_tag="AC16YU_07980"
     CDS             1216..4713
                     /locus_tag="AC16YU_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065201.1"
                     /translation="MHTTILNESGHPEQSLRPAPTTMMPSKGILRSLGLPALVATVLV
                     GLAFATPATAQEGTTIPPEYRGTDVSIARGILDGNLIETNFRNHGELARWNDLPWGIW
                     PRSIGGRHIDGIAVLVVGAVLGERAKWTAPPYEFWQPGTPDTLIHPVSVKYRDAGTFV
                     GPDGGIWGWLPLPGFFNELRRNPVTKQREPTPALSDDPTSWPEFWPDKLQEEDSGWPG
                     FWNGMGGKSVFSADLESFYVMDDYNDANYHIRRSTGRPYSQFGVFYPDPSDSTKGGMA
                     LQMNVRILQWANILAEDTMFLIYRITNTGGTDYGALVNTSQGVEKTGLWFAQFIDYGL
                     GNEEGDESAEFNPQLDIAFGFDHDGIGQHQAGGTYELGYTGFAFLESPRRDDDALDND
                     EDGITDEQRFGGPGIRVEGQEQIRAMVEASYRMDLFEEHYGPLEDRPAFRRGIWWTGD
                     DDLDWVGYTDINENGQYDAGELVNDDYGRDGLGPFDLAYDGPDDGEADGIPTIREPNF
                     DELDVDESDQIGLTGFHLGTRPYYESGDNLRSDTWMWARIAESQFALGTDPETIRAGV
                     EPFLNFSSGPVDLRNQETDFFSTAWLFGENRADFFKNREIVQRIYDADYRFAQPPIVP
                     KLSAEAGDEFVILSWDTLSVASYDRFTQEFDFEGYKLFKGTDPLLSDARLVTDAYGIK
                     KYNKPLAQWDLANGIRGTVPVLENTAFYDMGSDTGLQFSYVDRDVINGRTYYYALVAY
                     DHGFTPSPDDLNPDAAPVDPQENTFNVSVDLGGEVRGFTQNVAVVTPRSQAAGYVRAS
                     TNEDLSHVTAGVGTGSAEVEVVTEASIRPDIVYRISFFDTTRGTADIYETTGFELVNV
                     TDNKVLIPRSPIVDQTPAVDGFVVRLQNDPPGVDNSMTGWEGLNDDGDVAWSALPGLL
                     QGYNTNWFVSIGPDDSNRYVESPYEYTVHFADSIYTSPSRRYTGYLVSQPLPMWCTNV
                     TLGTPCDIFPRDLNANGTLEWTEDAFVVADPAIAVWRFRHVIRFTAFGQSVEPEPGNL
                     LRISSTRPFSRDDYFQFSLAPGRVDEALAADEMEQIAVVPNPYIVAASWEPRTQIQGR
                     GPRMVQFIHLPQTCTIRIYTIRGELVRTLRHAGVGGDGAEWWDLQTENGQDVAYGIYL
                     FHVEAEGIGEKVGKFAIIK"
     gene            4764..5828
                     /locus_tag="AC16YU_07985"
     CDS             4764..5828
                     /locus_tag="AC16YU_07985"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6065202.1"
                     /translation="MIQSSLPYSRGLRMLGLAVLIAGLAHPASAQSRVSTSAATFLTI
                     GTGARGSALGHAYTATATGADALAWNPAGAARLGGMAQRGGVFFSHADWLVDIDYNAF
                     GIVIPITRSGVMGLNLVQMDYGRMDVRTVELPEGTGETFGAADLMIGMSYAQPLTDRF
                     YIGGTAKYVSQKIYDMSASTFAFDIGFVLESDYFNGLRLAASIMNFGGLMQMEGVNNR
                     VFTDIDETNSGSNDAIPATLETTKWNLPLSFKFGAALPVVKASYASLELLADAHQTND
                     NDLNVDLGSELRFRFDPVVLDLRVGHKDAGLGSQVLSHWTFGGGVDLSLGDLRFGADY
                     GYVPFDALGNVSVLDFRVYF"
     gene            5903..>7179
                     /locus_tag="AC16YU_07990"
     CDS             5903..>7179
                     /locus_tag="AC16YU_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065203.1"
                     /translation="MKRWFTTALFTLIAIAVAGALPAAAQDVYLRGGGVNTSYGAPNY
                     PLISGFESIEEDTTLWGQYGFWDEGSDCRYQGPPCWDFYEGQRDYAILFDYKDAGYND
                     APQGESAVLIDWGVFSDNSWGGSSAFQHQLPDSVITAGTFYDFSDFTEIGIDYNNLAP
                     TGEGALFRIKINEASEGDRVTAGGFEDWYSESNVMLEAQPGWGEVFIPLEMVGGCCGT
                     GGFNRPGWSGVTGNDFFDTDKIAGFQLEFVAPSSATAGDSAIAGTVVWDNFRVRGERY
                     GVNEAFEGAADGSWKNSTAGSFTITASDDHVEGSQSIQVDFNVVGDQGWGGNVEIQGN
                     AEGGYYPVAASNTHISLFYRVLTPSALPEAILTMKIFDESNGEKEQWNYETKGVPNDD
                     SGQWRRLLIPLKDVFHPSWEPLRDGVFDQTKIS"
BASE COUNT         1294 a         2392 c         2348 g         1145 t
ORIGIN      
        1 ccagctgggt gcccgtgccg tcggtgaacc ggtacggccg cgacacgctc cagacgcggc
       61 cctacgaact ggccgcgtac atccaggaca agatggaatt ccggaacctg atcgtgaacg
      121 ccggtatccg cttcgactac ttcgagcccg accgccagat tccggtgcag tgggaggagg
      181 cggggagcat ctgggtaccg gtgtacgacg acaacgacgt cgcgaccgga gattcgctct
      241 acaaccgcga ggacggtccg gcacggtggc agatcagccc gcgcatcggc atcgccttcc
      301 cgatctccgc gacgggcgtg atccgcttct cggcgggcct cttcttccag atcccgcagc
      361 tgtccatcct cttcacgaac ccgaactacg aggtcaaccc ggcgtccgcg aacatccagt
      421 tcggcaaccc ggcgctggat ccggagcgca cgctccactt cgagatcggt ctgcagcagc
      481 agctcaccga cgagatgggg ctcgagctca ccctcttttc caaggacatc cggaacctga
      541 cgggcgtcga aatacggagg gacgtcgaga cgacgagtac cacggtccgg tacatcaacc
      601 gggacgtagg cacttcgcgt ggcctcacgc tctccttgtt ccagcgcccg atcggcggca
      661 tttcctggga cctggactac acgctgcagt tcgcggacgg cacctcgtcg aatccgaccg
      721 aggcgttcgg gcggttccag agcggacagg aagagatcct cacgctcgtg cggctcgact
      781 gggacaggcg gcacgtgctg accaacagca tcaccgtgac ccccgcccag gcgttctccg
      841 tcaccctgat caatcggtat cagaccggcg cgccctacac gacggaacgc cagttcgtca
      901 cgtcgtacat caggaacaac gcggaccggc ccgcgggctt cacctcggac ctgcggctgt
      961 tctaccgccc gtggttcgtg tcggggctgt cgctgaccgc gcaggtcgac aacctgttcg
     1021 acgcgatgat ccactacgga gtctacaacg actccggacg gggcgacgag acggtctcga
     1081 tggagctcta ccgccggtcg ggcaccccca tcggcggcgt caactcgctc gacgagtatt
     1141 tcttcaacca gggctggttc tcggtgccgc gccgtgtaac ggtcggcctg cgctacgact
     1201 tctgacccgg accccatgca cacgaccatc ctgaacgaat cgggccatcc ggagcagtcg
     1261 ctccgtcccg caccgaccac catgatgcca tcgaaaggga ttctgcgatc gctcgggctg
     1321 ccggcgctgg ttgcgaccgt cctggtcggc ctggcgttcg ccacgccggc aaccgctcag
     1381 gagggcacca cgattccgcc cgagtatcgc ggcaccgacg tctcgatcgc gcgcggcatc
     1441 ctcgacggca acctgatcga gacgaatttc cgcaaccacg gcgagctggc ccggtggaac
     1501 gacctcccct ggggaatctg gccgcggtcg atcggcggcc gccacatcga cggcatcgcc
     1561 gtgctggtgg tcggcgccgt gctcggcgag cgggccaagt ggacagcgcc gccctacgag
     1621 ttctggcaac cgggcacgcc ggacaccctc atccatcccg tctcggtgaa gtaccgggac
     1681 gccggcacct tcgtcggtcc ggacggcggc atctggggct ggctcccgct tccgggcttc
     1741 ttcaacgagc tccggcgcaa cccggtgacc aagcagcggg agccgacgcc ggcgctcagc
     1801 gacgacccga cctcctggcc ggagttctgg cccgacaagc tgcaggagga ggattccggc
     1861 tggccgggat tctggaacgg catgggaggc aagtcggtgt tcagcgccga cctggagtcg
     1921 ttctacgtga tggacgacta caacgacgcc aactaccaca tccgcaggtc gaccggacgt
     1981 ccgtacagcc agttcggcgt cttctacccg gatccgtccg attccacgaa gggcggcatg
     2041 gcgcttcaga tgaacgtccg catcctgcag tgggcgaaca tcctggccga ggatacgatg
     2101 ttcctgatct accggatcac gaacaccggc ggcacggact acggcgcgct cgtgaacacg
     2161 tcgcagggcg tggagaagac cggcctgtgg ttcgcccagt tcatcgacta cgggctcggc
     2221 aacgaggagg gagacgagtc cgccgagttc aacccgcagc tcgacatcgc cttcgggttc
     2281 gaccacgacg gcatcgggca gcaccaggcg ggcggcacct acgagctcgg ctacaccggt
     2341 ttcgccttcc tcgagagccc caggcgggat gacgacgccc tggacaacga cgaggacggc
     2401 atcaccgacg agcagcgttt cggcgggccc ggcatccggg tcgagggcca ggaacagatc
     2461 cgcgccatgg tggaggcgag ctaccggatg gacctgttcg aggagcacta cggcccgctc
     2521 gaggaccgcc cggccttccg gcgcggaata tggtggaccg gggacgacga cctggactgg
     2581 gtcgggtata ccgacatcaa cgagaacggg cagtacgacg ctggagagct cgtcaacgac
     2641 gactacggac gggacggcct cggacccttc gacctggcct acgacggtcc ggacgatggc
     2701 gaggccgacg gcatcccgac gatccgggag ccgaacttcg acgagctcga cgtggacgag
     2761 tcggaccaga tcggtctgac cggcttccac ctgggcacgc ggccctacta cgagagcggc
     2821 gacaacctgc gctccgacac gtggatgtgg gcccggatcg ccgagtcgca gttcgcgctc
     2881 ggtacggacc ccgagaccat ccgcgcaggc gtcgagccct tcctgaactt cagttccggc
     2941 ccggtcgacc tccgcaacca ggagaccgat ttcttctcga cggcctggct gttcggcgag
     3001 aaccgggctg acttcttcaa gaaccgcgag atcgtccagc ggatctacga cgcggactac
     3061 cgcttcgcgc agccgccgat cgtgccgaag ctcagcgccg aggcaggcga cgagttcgtg
     3121 atcctctcgt gggacacgct gtccgtcgcg agctatgacc ggttcacgca ggagttcgac
     3181 ttcgaggggt acaagctctt caaggggacg gacccgctgc tttcggacgc ccggctggtc
     3241 accgacgcat acggcatcaa gaagtacaac aagccgctcg cccagtggga cctcgcgaac
     3301 gggatccgcg gcaccgtccc cgtgctcgag aacaccgcgt tctacgacat gggctccgac
     3361 acggggctgc agttctcgta cgtggaccgg gacgtgatca acgggcgcac ctactactac
     3421 gcgctcgtag catacgacca cggcttcacg cccagcccgg acgacctgaa cccggacgcg
     3481 gccccggtcg acccgcagga gaacacgttc aacgtgagcg tggacctcgg tggcgaggtg
     3541 cgcggcttca cccagaacgt ggccgtcgtg acgccgcgct cgcaggcggc gggctacgtc
     3601 cgggcatcga cgaacgagga cctctcgcac gtcacggcgg gcgtcggcac gggttcggcc
     3661 gaggtcgagg tggtgaccga ggcgagcatc cgcccggaca tcgtgtaccg catttcgttc
     3721 ttcgacacca cgcgcggcac ggccgacatc tacgagacga cgggcttcga gctggtcaac
     3781 gtgacggaca acaaggtgct gattccgcgg tcgccgatcg tggaccagac gcccgcggtg
     3841 gacgggttcg tggtccggct gcagaacgac ccgcccggcg tggacaacag catgaccgga
     3901 tgggaggggc tcaacgacga cggagacgtc gcctggagcg cgctgcccgg cctgctgcag
     3961 ggctacaaca cgaactggtt cgtcagcatc ggaccggacg actcgaaccg gtacgtggag
     4021 tcgccctacg agtacaccgt ccacttcgcg gactcgatct acacgagtcc cagccggcgg
     4081 tacacggggt acctggtgtc gcagccgctg ccgatgtggt gcacgaacgt gacgctgggt
     4141 acgccctgcg acatcttccc gcgcgacttg aacgccaacg ggacgctcga gtggaccgag
     4201 gatgcgttcg tggtcgcgga tccggcgatc gccgtgtgga ggttccgcca cgtgatccgg
     4261 ttcacggcat tcgggcagag cgtcgagccc gagccgggca acctgctccg catctcttcc
     4321 acccgcccgt tctcccgcga cgactatttc cagttctcgc tggcgccggg ccgggtggac
     4381 gaggcgctgg ccgccgacga gatggagcag atcgccgtcg taccgaaccc ctacatcgtg
     4441 gcggcgtcgt gggaaccgcg cacgcagatc caggggcgcg gcccgcgcat ggtccagttc
     4501 atccacctgc cgcagacgtg caccatccgg atctacacga tccggggcga actcgtccgg
     4561 accctgcgtc acgccggcgt gggaggtgac ggcgcggagt ggtgggacct gcagaccgag
     4621 aacgggcagg acgtagcgta cggcatctac ctcttccacg tcgaggcgga aggcatcggc
     4681 gaaaaggtgg gcaagttcgc catcatcaag tgagcggtca cgtggaacct accaatcgtt
     4741 cggatcgagg caaggaacgc atcatgatcc aatcatcgct cccatactcc cgaggcctcc
     4801 ggatgctggg cctggcggtg ctcatcgcag gactcgcgca tccggcgagt gctcagagcc
     4861 gcgtcagcac gtcggccgcc acgttcctga ccatcggcac gggcgcgcgg ggcagtgcgc
     4921 tcgggcacgc ctacaccgcc accgccaccg gggccgacgc gctcgcgtgg aatccggccg
     4981 gcgccgcgcg gctcgggggc atggcgcagc ggggcggggt cttcttctcg cacgccgact
     5041 ggctcgtgga catcgactac aacgccttcg gcatcgtcat cccgatcact cgctcgggcg
     5101 tgatggggct gaacctggtc cagatggact acgggcggat ggacgtgagg acggtcgagc
     5161 tgcccgaggg cacgggcgag acgttcgggg cggcggatct catgatcggc atgagctacg
     5221 cgcagccgct gaccgaccgc ttctacatcg gcggcacggc caagtacgtg agccagaaga
     5281 tctacgacat gtcggcgtcg actttcgcgt tcgacatcgg cttcgtgctc gagtccgact
     5341 acttcaacgg gctgcggctg gccgcgtcga tcatgaactt cggcgggctc atgcagatgg
     5401 agggcgtcaa caaccgggtg ttcacggaca tcgacgagac gaactcggga agcaacgacg
     5461 ccattccggc cacgctcgag accacgaagt ggaatctgcc cctgtcgttc aagttcggcg
     5521 cggcccttcc cgtggtgaag gcgtcctacg cgagcctgga gctcctggcc gatgcccacc
     5581 agaccaacga caatgacctg aacgtggacc tgggcagcga gcttcgtttc aggttcgacc
     5641 ccgtcgtgct cgacctcagg gtaggacaca aggacgcggg gctcggcagc caggtcctgt
     5701 ctcactggac gttcggcgga ggcgtcgatc tctcgctcgg cgatctccgc ttcggggcgg
     5761 attacggcta cgtgccgttc gacgccctgg gtaacgtgag cgtgctcgat ttccgggtat
     5821 acttctgaca ccggcacccg cccccttcgg ggagtgcccg caacagttcg aaaggacttt
     5881 caccatcaca cggaggcaca ccatgaaacg atggtttacg acggccctgt tcaccctgat
     5941 cgcgatcgcc gtcgcgggcg cccttccggc ggccgcgcag gacgtatacc tgcggggtgg
     6001 cggggtcaac acgagctacg gcgcgcccaa ctacccgctc atctcgggat tcgagtcgat
     6061 cgaggaggac acgacgctct ggggccagta cggcttctgg gacgagggga gcgactgccg
     6121 ctaccagggt cccccgtgct gggacttcta cgagggccag cgtgactacg cgatcctctt
     6181 cgattacaag gatgccgggt acaacgacgc accccagggc gagtcggctg tcctgatcga
     6241 ctggggcgtg ttctcggaca acagctgggg tggctcgagc gcgttccagc accagctgcc
     6301 ggactccgtc atcacggcgg gcacgttcta cgacttctcg gacttcaccg agatcggcat
     6361 cgactacaac aacctcgcgc cgaccggcga gggtgccctc ttccgcatca agatcaacga
     6421 ggcgagcgag ggtgaccgcg tgactgccgg gggcttcgag gactggtact ccgaatccaa
     6481 cgtgatgctc gaggcccagc ccggctgggg cgaggtcttc attccgctgg aaatggttgg
     6541 tggatgctgc ggcaccggcg gcttcaaccg tccggggtgg agcggcgtca ccggcaacga
     6601 ctttttcgac acggacaaga tcgcgggctt ccagctcgag ttcgtggctc cgagctcggc
     6661 cacggcgggc gactcggcga tcgccggcac ggtggtctgg gacaacttcc gcgtgcgcgg
     6721 cgagcgctac ggcgtgaacg aggccttcga gggcgcggcc gacggctcgt ggaagaactc
     6781 caccgcaggc tcgtttacga tcacggccag cgacgaccat gtcgagggat cgcagtccat
     6841 ccaggtggat ttcaacgtcg tgggcgacca aggctggggc ggcaacgtgg agatccaggg
     6901 gaatgccgaa ggcggttact accccgtcgc ggcctccaac acgcacatca gcctgttcta
     6961 cagggtgctg acgccgtccg cgcttcccga agccatcctg acgatgaaga tcttcgacga
     7021 gtcgaacggc gagaaggagc agtggaacta cgagaccaag ggcgtaccga acgacgacag
     7081 cggccagtgg cgtcggctcc tcatcccgct caaagacgtg ttccatccga gctgggagcc
     7141 gctccgggac ggcgtcttcg accagacgaa gatctccga
//
LOCUS       DBNHQZ010000299         7060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1716285, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000299 DBNHQZ010000000
VERSION     DBNHQZ010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1716285"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="AC16YU_07995"
     CDS             <1..483
                     /locus_tag="AC16YU_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR6065204.1"
                     /translation="DFAEIFAAAQIEAGCDIIGIGDAVCSQVDPGTYETFVKHRHRRL
                     IDHIHARGGLVKFHVCGDITHLLPAVAELGVDLIDLDWPVDIAGARAVLGPEVVITGN
                     LDPSLVLSRDRETVHRMARDLVRAHAGTRYILAGGCEIGVDTPPENLLAMGRAAREGA
                     "
     gene            488..1858
                     /locus_tag="AC16YU_08000"
     CDS             488..1858
                     /locus_tag="AC16YU_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HIR6065205.1"
                     /translation="MRYSELAIPDPDDLVFGRAPHPVRSRRGLVFGGGTVYPELNFTL
                     PPTRITRDGLPGLEAQYRGIVDGALNRAVELGSRGVVVEFETLVEMTRDPDIGVALVR
                     VMDEVCERYAREHGLPTALRLTPNDLREFERPPRMRTGGLLAPMMELFERGAEAGGDL
                     LSIESTGGKEVSDEALMQCDMGLFVFSQAVLGARDVRMLWTRIVDIAARTGRIAGGDT
                     ACAFGNTAMVLAERRFIPRTVAAVARVATVVRTLAAVEAGALGPDKDCGYEGPYLKAI
                     AGIPISMEGRTAACAHGSPVGNVAAACADLWSNESVQNVMLLADYAPTVSMEQLEYDT
                     RLMNVATARGEAHVLRDLLVASDRGLDPQALVLDPDVVVPVAREIVRASNPVEAARNA
                     CLRALDLIEDASRDGTLRLDAIERPWLGTLRDDLESIPDTEDAFVERMQPRLDPSKLI
                     LAEYGL"
     gene            1861..2535
                     /locus_tag="AC16YU_08005"
     CDS             1861..2535
                     /locus_tag="AC16YU_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860106.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR6065206.1"
                     /translation="MDDLLARIAHCIEFGKIDRQSPYPPDLRGQDGADELTREALAQG
                     TTPERLLEEAFVVGMDRVGRKFAERRIFVPQMLMAARAMSAAMHHLKPYFASGSVQRR
                     GTFVIGTVTGDLHDIGKNLVAMMVEGSGWEVVDLGVDVGAERFAEEVARRDGRLAVGL
                     SAMLTTTMLSMRDIVADLRARYPELPILVGGAPVNDGFRAEIGADFYSRDPQGAVDYL
                     NGLAAA"
     gene            2532..3260
                     /locus_tag="AC16YU_08010"
     CDS             2532..3260
                     /locus_tag="AC16YU_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR6065207.1"
                     /translation="MRRTEIIAGREYATYAFDLADLDLQASYVERVMHHDPQDPSGPW
                     TELIEETLREAAGRCAIEGGYTIVDGPEFDESVRTLRLGGELFHLEKIVFGQIRKAER
                     VALFLCTAGPIGEWSRELMRAGDPLRAYVVDVVGSEIVEFAVDRIHHDLGRRFADEGL
                     RITNRFSPGYCNWDVREQQRLFRSFPPDYAGIRLSESSVMHPTKSVSGIVGIGHRVKL
                     NPYLCNVCSEEMCIYRDKRLLDDR"
     gene            3389..4675
                     /locus_tag="AC16YU_08015"
     CDS             3389..4675
                     /locus_tag="AC16YU_08015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6065208.1"
                     /translation="MSDSAYNPFVAAQRQFDHAAEILGLDRGTRDLLREPLREYAISI
                     PVRMDDGHVQVFRGFRVQHNDARGPSKGGIRFHPQETLDTVRALAMWMTWKCALVDIP
                     LGGGKGGVVCDPHNLSEREQERLCRGWVRQLARDMGPEQDVPAPDVMTSAQHMLWMLD
                     EFETIKGARKPGFITGKPVGMGGSLGRTEATGFGLVMTLREALKELGIRAADTTASVQ
                     GFGNVARYAIQLYQELGGTVTAVATWNQKDSAAYTFRRAEGINLDLLLSITDHFGEVD
                     RARAVEAGLEVLQGDAWLEQDVDILMPCALENQINDDTVGRISKRVKVVAEGANGPTT
                     ASAGRELDKRGVFVIPDFLANAGGVTCSYFEQVQSNMNYYWERDEVLQKLDLKMTAAF
                     AAVSNLARTRKLSTREAAYVIAVDRVARACRDRGWV"
     gene            4767..>7060
                     /locus_tag="AC16YU_08020"
     CDS             4767..>7060
                     /locus_tag="AC16YU_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6065209.1"
                     /translation="MTGRRESMEDVRQPLSEDGKERGGPSEWAIVLDFLRRADPPLFA
                     TITRKMINYLCWSGVEDAIDLLREYLSADLTEEEVASEPNRPAARSEPHEIAPQSERA
                     FGIASEHCSDEEIVGLIRSWIEEDKAAGLVETLENPQHGFPEVAASLERFLAADADEP
                     AISRPLKRSLGASLVRRFFSDDLDLLGILKDRVRVRDFASLVRRVVYTSTSRGRLGGK
                     STGLFMADVILRDSASDKPELARIRIPKTWYVTSDGVLEFIRHNNLDDVHSHKYQDID
                     RVRRDYPHLVSVFKNSPFPTELINGIAAALDDLGDGPLIVRSSSLLEDQIGAAFSGKY
                     KSLFLANQGTKSERLAALLDAVAEVYASIFAPDPIEYRAERGLLDVNEEMAVMIQEVV
                     GVRVGRYFLPAFSGVAFSRNDFRWSPRIRREDGLIRLVPGLGTRAVDRIGDDYPVLVA
                     PGQPGLRVNVTTDEIVRYSPRKADVIDLEGDGFETVDVESLLREVRDEYPMVRQVVSI
                     VTDNHVRRPVGLEPDWDRDRIAVTFAGLVEDESFTRTIRNLLDHLCEEMGHPVDIEFA
                     CDGTDLYLLQCRAQSASVESDPTPIPRHLSPDDVVFTADRYVSNGRVPDITHVVYVDP
                     QAYAELESLSDLKEVARAVGRLNKLLPHRQFMLIGPGRWGSRGDIKLGVGVTYADINS
                     TAALLEVAWTRGNYVPELSFGTHFFQDLVEADIRYLPLYPDEPGVVFNRGFFTDTPSI
                     LTTLLPEYAHLERVVRVVDVPATTG"
BASE COUNT         1119 a         2402 c         2489 g         1050 t
ORIGIN      
        1 gacttcgccg agatcttcgc ggcggcacag atcgaggccg gttgcgacat cataggcatc
       61 ggcgacgcgg tctgctcgca ggtcgatccc gggacctacg agaccttcgt aaaacatcgt
      121 catcgccggc tcatcgacca catccacgct cggggaggcc tcgtcaagtt ccacgtctgc
      181 ggggacatca cgcacctgct tccggccgtc gcggagctcg gcgtcgatct catcgacctc
      241 gactggcccg tggacatcgc cggcgcgcgc gccgtactcg gcccggaggt cgtcatcacc
      301 ggcaacctgg atccgtccct ggtgctgtcg cgggaccggg aaacggtcca ccgcatggcc
      361 cgggacctgg tccgggccca cgccgggacg cggtacatcc tcgcgggcgg gtgcgagatc
      421 ggcgtcgaca cgccgcccga gaacctgctc gcgatgggcc gggccgcccg ggagggcgcc
      481 tgacgccgtg cggtacagcg agctcgccat ccccgatccg gacgacctcg tgttcggacg
      541 cgctccccac ccggtccgga gccggcgcgg gctcgtgttc ggcggcggga ccgtgtatcc
      601 cgagctcaat ttcacgctgc ccccgacgcg gatcacccgc gacggcctgc ccgggctcga
      661 ggcgcagtac cgcggcatcg tggacggcgc cctgaaccgg gccgtcgaac tcggcagccg
      721 gggcgtggtc gtcgagttcg aaacgctcgt cgagatgacc cgtgatccgg acatcggggt
      781 ggcgctcgtc cgcgtgatgg acgaggtgtg cgagcggtat gctcgggagc acgggcttcc
      841 gacggcgctc cgcctgacgc ccaacgacct gcgcgaattc gagcggcccc ctcgcatgcg
      901 gacgggcggg cttctcgccc cgatgatgga gctgttcgag cgcggggccg aggcgggggg
      961 cgacctcttg tccatcgaat ccaccggagg caaggaggtg agcgacgagg cgctcatgca
     1021 gtgtgacatg gggctcttcg tgttctcgca ggccgtgctg ggggcgcgcg acgtccggat
     1081 gctctggacg cggatcgtgg acatcgcggc gcgcaccggc cgcatcgcgg gcggcgacac
     1141 ggcctgcgcg ttcggcaaca cggccatggt gctcgccgag cggcgcttca ttccgaggac
     1201 ggtggcggcc gtcgcacgtg tcgcgaccgt cgtccggacc ctcgcggccg tggaggccgg
     1261 ggccctcggg cccgacaagg actgcggcta cgaggggccg tacctgaagg ccattgccgg
     1321 tatcccgatc tcgatggagg ggcggacggc cgcgtgcgcg cacgggagcc cggtgggcaa
     1381 cgtcgccgcg gcctgcgcgg atctctggag caacgaatcc gtccagaacg tgatgctgct
     1441 cgcggactat gcccccacgg tctccatgga acagctcgag tacgacacgc gcctcatgaa
     1501 cgtcgccacc gcccgtggcg aagcccacgt gctgcgcgac ctgctggtgg cgtcggaccg
     1561 ggggctcgat ccgcaggccc tggtgctcga ccccgacgtc gtggtcccgg tcgcccgcga
     1621 gatcgtccgg gcctcgaatc ccgtggaggc ggcgcgcaac gcctgcctga gggcgctcga
     1681 cctcatcgag gacgcctcgc gcgacggcac gctgcggctg gacgccatcg aacggccctg
     1741 gctcgggacc ctgcgggacg atctcgagtc catacccgac acggaagacg cgttcgtcga
     1801 gcggatgcag ccccgcctcg atccctccaa actgatcctg gccgaatacg gcctgtgacg
     1861 atggacgacc tgctcgcgcg catcgcgcac tgcatcgagt tcggcaagat cgaccggcag
     1921 tcgccctacc cgccggacct gcgcggacag gacggcgccg acgagctgac ccgcgaggcg
     1981 ctcgcgcagg ggactacgcc ggagcggctg ctcgaggagg ctttcgtggt cgggatggac
     2041 cgcgtagggc ggaagttcgc cgagcggcgg atcttcgtgc cccagatgct catggcggcc
     2101 cgggccatga gcgccgccat gcatcacctg aagccgtatt tcgcctcggg cagcgtgcag
     2161 cggaggggga ccttcgtgat cgggaccgtg accggcgacc tgcacgacat cggcaagaac
     2221 ctggtggcca tgatggtgga ggggtcaggc tgggaggtcg tggacctggg cgttgacgtg
     2281 ggcgcggagc ggttcgcgga ggaggtcgcc cggcgggatg gccgcctggc cgtcgggctc
     2341 tccgcgatgc tgacgacgac gatgctcagc atgcgcgaca tcgtggccga cctgcgggcc
     2401 cggtaccccg aactgcccat cctggtgggc ggagcgcccg tcaacgacgg gttccgcgcg
     2461 gagatcggag ccgacttcta ttcccgcgat ccgcagggcg ccgtcgatta cctgaatgga
     2521 ctcgccgcgg cgtgaggcgc accgagatca tagccgggcg cgagtacgcc acgtacgcct
     2581 tcgatctcgc ggacctggac ctgcaggcgt cgtacgtgga gcgcgtcatg caccacgacc
     2641 cgcaggatcc gtccggcccc tggaccgagc tcatcgagga gacgcttcgc gaggccgccg
     2701 gccgctgcgc gatcgagggg ggatacacga tcgtcgacgg accggaattc gacgagtcgg
     2761 tgcggacgct ccgcctcggg ggcgagctat tccatctcga gaagatcgta ttcgggcaga
     2821 tccgcaaggc cgagcgcgtc gccctgttcc tgtgcaccgc cgggccgatc ggggagtggt
     2881 cccgcgaact gatgcgggcg ggggacccgc tcagggccta cgtggtggac gtcgtcggct
     2941 ccgagatcgt cgaattcgcc gtcgaccgca tccaccacga tctcgggcga cggttcgcgg
     3001 acgaggggct ccggatcacg aaccgcttca gccccgggta ctgcaactgg gacgtgcgcg
     3061 agcagcagcg actgttccgc tcctttccgc cggactacgc gggaatccgc ctgtccgaga
     3121 gctccgtgat gcatcccacg aagtcggtca gcggcatcgt gggcatcggc caccgggtga
     3181 aactgaaccc ctacctgtgc aacgtctgtt cggaggaaat gtgtatctac cgggacaagc
     3241 ggctgctcga tgaccggtga gcccgttgtc ctcgcgcgcc ctacggccgg gcgaggggga
     3301 atcctacacc ctgttccgcc ctttatctgc tgacccggga aagcgcttcc ggtaacatgg
     3361 gcgcgcactg gaaaccacgt ccaagaccat gtcagactcg gcatacaatc ccttcgtcgc
     3421 cgcccagcgg cagttcgacc acgccgcaga aatcctcggc ctggaccggg gcacccgcga
     3481 cctgctgcgg gagcccctgc gcgagtacgc aatctcgatc ccggtccgga tggacgacgg
     3541 gcacgtgcag gttttccgtg gcttccgcgt gcagcacaac gacgcccgcg gcccctcgaa
     3601 gggcggcatc cgtttccacc cccaggagac gctggacacg gtgcgagcgc tcgccatgtg
     3661 gatgacctgg aagtgcgccc tcgtggacat tccgctcggc ggcggcaagg gcggggtggt
     3721 gtgcgacccg cacaacctga gcgagcggga gcaggagcgg ctttgccgcg ggtgggtgcg
     3781 ccagctcgca cgcgacatgg gacccgagca ggacgtgccg gcgccggacg tcatgacttc
     3841 ggcccagcac atgctctgga tgctggacga gttcgagacg atcaagggcg cgcgcaagcc
     3901 gggcttcatc accggcaagc cggtcggcat gggcggctcg ctcgggcgca cggaggccac
     3961 ggggttcggc ctcgtgatga cgctgcgcga ggcattgaag gagctcggga tccgggcggc
     4021 cgacacgacg gccagtgtcc agggattcgg caacgtggcc cgctacgcca tccagctcta
     4081 ccaggagctc ggcggcacgg tgacggcggt ggccacctgg aaccagaagg actcggcggc
     4141 ctacacgttc cgccgcgcgg agggcatcaa cctggacctg ctgctctcga tcacggacca
     4201 cttcggcgag gtggaccggg ccagggcggt cgaggccgga ctcgaggtgc ttcagggcga
     4261 cgcctggctg gagcaggacg tggacatcct gatgccgtgc gccctcgaga accagatcaa
     4321 cgacgacacc gtggggcgga tcagcaagcg ggtgaaggtc gtcgccgagg gcgccaacgg
     4381 cccgaccacg gcctcagcgg gtcgcgagct ggacaagcgg ggcgtgttcg tgatcccgga
     4441 cttcctggcg aacgcggggg gcgtgacgtg cagctatttc gagcaggtcc agagcaacat
     4501 gaactactac tgggagcgcg acgaggtgct gcagaagctc gacctcaaga tgaccgcggc
     4561 gttcgcggcc gtctcgaacc tggcgcggac gcggaagctg tccacgcgcg aggccgcgta
     4621 cgtgatcgcc gtggaccgcg tggcccgggc gtgccgcgac cgcggatggg tctgaccggc
     4681 tgcaggggag gggccccgac cgcggccccg ggggccggag cgggaacccg accggtgatg
     4741 ccgggtgtcg gaccgtgcaa aacgacatga ccggcaggcg cgagtccatg gaagacgtcc
     4801 ggcaaccgct gtccgaggac gggaaggagc ggggagggcc cagcgagtgg gccatcgtcc
     4861 tcgactttct ccgccgggcc gatcctccgc tcttcgcgac gatcacgcgg aagatgatca
     4921 actacctgtg ctggagcggc gtcgaggacg cgatcgacct cctgcgcgag tacctgtccg
     4981 cggacctgac cgaggaggag gtcgcctcgg agcccaatcg cccggcggcg cggagcgaac
     5041 cccatgaaat cgccccgcag tccgagcgcg ccttcggcat cgcgagcgag cactgcagcg
     5101 acgaggagat cgtcggcctg atccgctcct ggatcgagga ggacaaggcg gccggcctgg
     5161 tcgagaccct ggagaatccg caacacggct tcccggaggt cgcggcgtcg ctggagcgct
     5221 tcctggccgc cgacgcggac gagccggcga tctccaggcc gctgaagcgc agcctcggag
     5281 catcgctcgt ccgccggttc ttcagcgacg acctggacct cctgggcatc ctcaaggacc
     5341 gggtccgcgt ccgggacttc gccagtctcg tccggcgggt cgtgtatacc tcgacgagcc
     5401 gcggccgcct cggcggcaag agcacgggcc tgttcatggc cgacgtgatc ctgcgcgact
     5461 ccgcatccga caagcccgag ctcgcccgga tccggatccc gaagacctgg tacgtgacct
     5521 ccgacggggt gctggaattc atccggcaca acaacctgga cgacgtgcac tcgcacaagt
     5581 accaggacat cgaccgggtg cgccgggatt acccgcatct ggtcagcgtc ttcaagaact
     5641 cgcccttccc gacggagctc atcaacggca tcgccgccgc gctcgacgac ctgggcgacg
     5701 ggccgctcat cgtgcggagc tcgagcctgc tcgaggacca gatcggggcc gcgttctcgg
     5761 ggaagtacaa gagcctgttc ctggcgaacc agggcaccaa gagcgagcgc ctggccgcgc
     5821 ttctcgacgc ggtcgccgag gtgtacgcgt cgatcttcgc tcccgacccg atcgagtacc
     5881 gggccgagcg cggactcctg gacgtgaacg aggagatggc ggtcatgata caggaggtcg
     5941 tgggtgtgcg cgtcggccgc tacttcctgc ccgcgttctc gggtgtcgcc ttctcgcgca
     6001 acgacttccg gtggtctccc cgcatccgca gggaagacgg gctgatccgc ctggtgcccg
     6061 gcctcgggac gcgcgccgtg gaccggatcg gcgacgacta cccggtcctc gtagcgcccg
     6121 gccagcccgg gctgcgcgtc aacgtcacga ccgacgaaat cgtgcgctac tcgccgcgga
     6181 aggccgacgt gatcgatctc gagggggacg gcttcgagac ggtggacgtg gagagcctgc
     6241 tgcgcgaggt gcgcgacgag tacccgatgg tgcgccaggt ggtatcgatc gtcacggaca
     6301 accacgtgcg ccgtcccgtc gggctggagc cggactggga cagggaccgc atcgccgtca
     6361 cgttcgccgg cctggtcgag gacgagtcgt tcacgcggac gatccgcaac ctgctcgacc
     6421 acctgtgcga ggagatgggg cacccggtgg acatcgaatt cgcctgcgac gggacggacc
     6481 tgtacctcct ccagtgccgg gcgcagagcg cgtccgtcga gtccgacccg acgccgatcc
     6541 ctcggcacct ttcgcccgac gacgtggtct tcacggccga ccgttacgtc tcgaacggtc
     6601 gcgtgcccga catcacgcac gtcgtgtacg tggatccgca ggcgtacgcg gagctcgagt
     6661 ccctgtccga cctgaaggag gtcgcgcgcg ccgtcggacg cctgaacaag ctgctgccgc
     6721 accggcagtt catgctgatc ggtcccgggc ggtggggcag ccgcggagac atcaagctgg
     6781 gcgtgggcgt cacgtacgcc gacatcaaca gcacggccgc cctgctcgag gtggcctgga
     6841 cgcgcggcaa ctacgtgccg gagctctcct tcggcacgca cttcttccag gacctggtcg
     6901 aggccgacat ccggtacctg ccgctgtacc ccgacgagcc gggggtggtc ttcaaccgcg
     6961 ggttcttcac cgacaccccg tcgatcctga ccacgctcct gcccgaatac gcccacctgg
     7021 aacgggtggt ccgcgtggtc gacgtgcccg ccacgacggg
//
LOCUS       DBNHQZ010000300         9417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1173049, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000300 DBNHQZ010000000
VERSION     DBNHQZ010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1173049"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC16YU_08025"
     CDS             <1..462
                     /locus_tag="AC16YU_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065210.1"
                     /translation="VLSISASRAQVPLGAHVQAGPAGLPGMGLQAGYVSAKTFYTREI
                     NLVADLSTFRASGDVQVAAAIGGSVRLLGIGRTIGNATYRGWDLDLGMRIGPGLLFEF
                     RESRAEKNRRFSLFLEPFVRTVARVRRAALFAEVGMKRPVLRAGFWVAVGR"
     gene            complement(463..1125)
                     /locus_tag="AC16YU_08030"
     CDS             complement(463..1125)
                     /locus_tag="AC16YU_08030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6065211.1"
                     /translation="MGLIAIGDIHGCARTLEALLERIGPTADDHIVFVGDYIDRGPDS
                     KGVIDLLLGLRTRQRCTFLRGNHEALFLNYLDEGEFDIWSYNGGTSTLDSYAVPGEGF
                     VIPEEHIAFIRETVMYLDTEDFFFVHAGLRPDRSIADNVRDADEMVYLWERGHLRMED
                     HELPWEKPVICGHTPVPEAVNRPKLINIDTGCVYFMRPTLGYLTAVRLPGREFVSVPF
                     MG"
     gene            complement(1125..1613)
                     /gene="nusB"
                     /locus_tag="AC16YU_08035"
     CDS             complement(1125..1613)
                     /gene="nusB"
                     /locus_tag="AC16YU_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6065212.1"
                     /translation="MSSRREAREAAMQALYAFELGGGEAQHVISTVIRERFADEPETR
                     EFATRLFLRTIDHAGDTDLLIAEHAQNWELSRIALVDRLVLRIAITELTSFEDIPPKV
                     TINEAIEVVKRFSTERSGQFVNGILDAVLVHLRERGGLNKRGRGLVGLDPATPGVAEA
                     KD"
     gene            1822..4032
                     /locus_tag="AC16YU_08040"
     CDS             1822..4032
                     /locus_tag="AC16YU_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR6065213.1"
                     /translation="MSLRPLPLVAAAFALGILAADRAWPVPLVPAVVAVGAAAILAGA
                     RPRLVGCRPLGRAAAAALLAATAGHFALASRLPPSGEALRAGEVECSGMLSSVRALGG
                     GRSELRLRRAACRRDGGLAVLGILSYADSAAVEAGAVVSLRGLLRRPGGFRNPGDPDR
                     RRIEERRGYGAVLALSRGSDVVVLRGPPPRSRRVARLRASVRAAVSVRFPDPAVRPLM
                     LALLTGDTSGIAEPVRDDFRRAGLAHVLAVSGLHVGLLAGAVLVVATSLLARLRVPLA
                     PRAVLTAALVVALLATLVLVIGPRPSVARAALMGSILVLRRLLQRPGQGLRDALALAM
                     VLQLAADPSVLFDPGFQLSHAAVFGLSFVPRGGGALRSGLRASAAATVATAPFVAWHF
                     GPVPLGGLVTNVAGLPLTAVVVTGGMLGLALSPVAGAADAAARALLFVAEVGADALGW
                     ARIVVPVGLVGTVLLATALSCTLARSARGRRVAAAAALVVAVLGPRPGTDLLRVVFLD
                     VGQGDAAVLHLPDGGSALVDTGTPAGSAPSRYLSRMGIDRVEHVVITHDHADHAGGLA
                     RTGETVSFGRVIGPGPVGGRPVHEVSAADTLDFGPSLRVYVLAPFGRGEGNEASLALA
                     VDFGRTRLLLMGDAEEAAERRIVEAWPDLLRSDVVKVGHHGSRTSSTPLLVRRAAARG
                     TLAVVSVGARNRYGHPAPGVLRRWAEAGAVVLMTSGRGAVCIESDGRGVREACP"
     gene            complement(4029..4868)
                     /locus_tag="AC16YU_08045"
     CDS             complement(4029..4868)
                     /locus_tag="AC16YU_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6065214.1"
                     /translation="MVKKILVALDPDSDTPVATRFALDIARRHDAEITGLAVVDMGSI
                     ESSSRGGGIGSMYYVEKLRDQLTADARSKAQELLDEFERLVRDSGVRFTELVQEGVPF
                     ERIVEDMKVHDLLIVGREPHFFYSHPKESTATLARIVRLTVGPTLIVHQDVRDVKKVL
                     IAYDGSTAAARAMRSFVLADPFGHEVNVRLVAIYRDTPTEAEFFLQQADDYLKAHGYD
                     AHRVTVLESDIDAALLAQIEEFGADLVVAGAHSKSTFRAMAFGSTTKNLVDHCPVPLF
                     LDS"
     gene            complement(5341..6618)
                     /locus_tag="AC16YU_08050"
     CDS             complement(5341..6618)
                     /locus_tag="AC16YU_08050"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR6065215.1"
                     /translation="MIHLDRSGVEPFLDDGAFRALHPRVLRAHETLLSGSGAGSDFLG
                     WRALLGEPNDALLGDIDDLAAEIRSRADVFLCIGIGGSYLGARAAISALTPYFPTPGH
                     GGPEILFAGHHMSGAYLRELLAHLEGRSVYVNVVSKSGTTLEPAIAFRVVREWMGRRF
                     EDAARRIVVTTDPEKGALNRLRASAGYRRYVIPPDVGGRFSVLSPVGLLPIAVAGVDI
                     RALFYGAVDARRSLASPDANPALDYAATRFLLHEAGFTTELLAVFEPRLSGLGAWWQQ
                     LFGESEGKEHKGLFPVPVQYSTDLHSVGQYVQDGRRNVAETFLLPDEDGGGLVIPGSP
                     DADDGLDFVAGRTLSLVNRKAAEGTIRAHVAGGVPVQVIRLPSIDPTSVGGLLYFFEH
                     AVSVSGYLLGVNPFDQPGVEAYKREMFALLGRP"
     gene            6676..7887
                     /locus_tag="AC16YU_08055"
     CDS             6676..7887
                     /locus_tag="AC16YU_08055"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR6065216.1"
                     /translation="MRTFVEVGEAREIVLSTAALLPVTHVPLAEAVGRTLAGDVVADM
                     DVPPFDNSAMDGYAVRAADAAAPGSVLRLSGGVRAGEWPSGAVDPGMCWGITTGAPLP
                     AGADAVVPVEQAEALGDGRIRLAAATEAGRHVRRAGRDVAAGQVVVTDGAAITPAVAG
                     ILAMVGMTRVPVRRPPVVAVVATGDELVPPDRRPGPGQIRNSNGPALAAQAVSAGAVV
                     LAPMHARDDAEATREVLRRALEADVLVFSGGVSMGSRDIVRDVLGEGGTEWLFWKVRQ
                     RPGKPLACGRLGARPVFGLPGNPVSSSVCFDQYVRPALLRMQGREPVFRPRYRAVLEE
                     ELPTAPGLHTFARGVAFAGEGPELRVRPTGPQGSNLFTSVLRANCLVHLPASRDVAPA
                     GSTVEIEWLAW"
     gene            complement(7891..8094)
                     /locus_tag="AC16YU_08060"
     CDS             complement(7891..8094)
                     /locus_tag="AC16YU_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR6065217.1"
                     /translation="MKKNMGAVDRAVRTVAAVVIALLVFLGVVQGTLATILVILAVVL
                     ILTSIVGTCPAYLPFGISTCRPA"
     gene            8287..9099
                     /locus_tag="AC16YU_08065"
     CDS             8287..9099
                     /locus_tag="AC16YU_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6065218.1"
                     /translation="MNTENIPLRSGRPRHEQVSTWLRDRIETGRYGPDDQLPSESRLV
                     DRFGVSRITVRRALQTLESEGLIYRRQGLGSFVRGERVRHGLVRLTDFAEDMELAGLH
                     ASSRVENRAIEPATARIAAALGVEEGAPVLRLDRVRLGNGDPIAFDRTWLPLFYAQLL
                     DGRDLGSETIYQILERDGGITMERGRFRFEAVNAPSDVGRALGVPWGRALLLVERVSY
                     AERGRCVYFQQRFYRCDRVAYELDLERESGATGPGLPIREFEPVFKATEDAG"
     gene            9171..>9417
                     /locus_tag="AC16YU_08070"
     CDS             9171..>9417
                     /locus_tag="AC16YU_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065219.1"
                     /translation="MRRALLVLVVSTLALPASGQDLNSPSGFLGYELGTRFTPHHRVL
                     EYVDHVAAASDRVRVERYGESVEGRPLALAFVASPENL"
BASE COUNT         1271 a         3244 c         3427 g         1475 t
ORIGIN      
        1 gtgctgtcaa tctccgcatc gcgggcccag gtgccgctcg gcgctcacgt gcaggcaggc
       61 cctgcgggcc tgcccggcat ggggcttcag gccggctacg tgtccgcgaa gacgttctac
      121 acgcgggaga tcaacctggt cgcggacctt tcgaccttcc gcgcgtccgg cgacgtgcag
      181 gtggcggcgg ccatcggcgg gtccgtccgc ctgctgggca tcggccggac catcgggaac
      241 gccacgtacc gcggatggga cctggacctg ggcatgcgca tcggaccggg cctcctcttc
      301 gagttccggg agagccgcgc cgagaagaac cgccgcttca gcctgttcct ggagccgttc
      361 gtccgtacgg tcgcgcgcgt gcggcgcgcc gcgttgttcg ccgaagtcgg gatgaagcgg
      421 cccgtgctgc gggccggatt ctgggtcgcg gtgggccgct gatcagccca tgaacggcac
      481 gctcacgaac tcgcgaccgg gcaggcgcac cgcggtcagg tagccgagcg tggggcgcat
      541 gaagtagacg cacccggtgt cgatgttgat gagcttcggc cggttcaccg cctcggggac
      601 gggcgtgtgc ccgcagatca cgggcttctc ccagggcagc tcgtggtcct ccatgcgcag
      661 gtgcccccgc tcccagaggt acaccatctc gtcggcgtcc cgcacgttgt ccgcgatgga
      721 gcggtcgggg cgcagcccgg cgtggacgaa gaagaagtcc tccgtgtcca ggtacatcac
      781 cgtctcccgg atgaacgcga tgtgctcttc cgggatcacg aagccctcgc ccggaacggc
      841 gtagctgtcg agcgtcgagg tccccccgtt gtagctccag atgtcgaatt cgccctcgtc
      901 caggtagttc aggaacaggg cctcgtggtt cccgcggaga aacgtgcagc gctgcctggt
      961 ccggagcccg agcagaaggt cgatcacgcc cttcgaatcc ggcccccggt cgatgtagtc
     1021 gccgacgaac acgatgtggt cgtccgccgt cggtccgatg cgctcgagca gcgcctcgag
     1081 cgtgcgcgcg caaccgtgga tgtcgccgat cgcgataagg cccatcagtc cttcgcttcc
     1141 gcgacgccgg gggtggcagg atcgaggccg acgagcccgc ggccccgctt gttcagtccg
     1201 ccccgttcac gcagatggac caggacggca tccaggatgc cgttgacgaa ctgcccggac
     1261 cgctcggtgc tgaaccgctt cacgacttct atggcctcgt tgatcgtgac cttgggtgga
     1321 atgtcttcga acgaggtcag ctcggtgatg gcgatgcgca gcaccaggcg gtcgaccagg
     1381 gcgatgcggg aaagctccca gttctgggcg tgctcggcga tgagcaggtc ggtgtccccg
     1441 gcgtggtcga tggtgcggag gaaaaggcgg gtggcgaact cccgcgtctc cggctcgtcc
     1501 gcgaaccgct cgcggatgac ggtggaaatc acgtgctgcg cctcgccgcc tccgagctcg
     1561 aacgcataca gcgcctgcat cgccgcttcc cgtgcttccc gtctggagct catcccgtca
     1621 ctctgtcaaa ttcggcggcg ggccgccggc gcttccctgg tttctggttc ctccgacagg
     1681 ataccccggc ccggtgcatc cggtcaacgg ctttcgtccg gtccgcgggc ggcggcggtc
     1741 gcgaggccag gagacgcgcc gcgtgacgtg gcggcgagac ccgaagcgtg acgcccggtg
     1801 cccgccgcgc ctccatccgg catgtcgctc cgccccctgc cgcttgtcgc cgccgcgttc
     1861 gccctcggca tcctggccgc ggaccgtgcc tggccggttc ccctcgtccc cgccgtggtc
     1921 gcggtcgggg cggccgccat tctggccgga gcgcgcccgc gcctcgtcgg gtgtcggccg
     1981 ctcggacgcg cggcagcggc ggccctcctc gccgcaacgg ccgggcactt cgcgctcgct
     2041 tcgcgccttc ctccgtccgg cgaggccctg cgcgcgggcg aggtggaatg ctcgggcatg
     2101 ctctcgtccg tgcgcgcgct cggcgggggt cgctcggaac tccgtcttcg ccgggccgcc
     2161 tgtcgaaggg acggcggcct ggccgtcctg ggaatcctgt cctacgccga ttcggcggcg
     2221 gtcgaggcgg gggccgtggt gtcccttcgc ggcctccttc gccgcccggg cggcttccgc
     2281 aatccgggcg atcccgaccg gcggcgcatc gaggagcggc gcgggtacgg cgccgtcctc
     2341 gcactctcgc gcgggtcgga cgtggtcgtc ctccggggac cgccgcctcg gtcgcgtcgc
     2401 gtggcacgcc ttcgggcctc ggtgcgggcc gccgtctcgg tacgcttccc ggaccccgcc
     2461 gtgcggccgt tgatgctcgc gctgctgacg ggcgacacct cgggcattgc cgaacccgtt
     2521 cgggacgact tccggcgggc cgggctcgcc cacgtcctgg ccgtttcggg cctgcacgtg
     2581 ggacttctcg ccggcgccgt gctcgtcgtc gccacgtcgc ttctcgcgcg ccttcgggtt
     2641 ccgctcgctc cacgcgccgt cctgaccgct gcgctcgtgg tcgcactcct ggccacgctg
     2701 gtcctcgtga tcggaccccg gccgtcggtc gcgagggcgg ccctcatggg cagcatcctc
     2761 gtgctccggc gcctgctgca gcgcccaggg cagggcctgc gcgacgcgct cgccctggcg
     2821 atggtgcttc agctcgccgc cgatccgtcc gtcctcttcg atcccgggtt ccagctctcg
     2881 catgcggccg tattcgggct ttcgttcgtt ccgcggggtg ggggcgccct gcggtcgggg
     2941 ctccgggcct ccgcggccgc gaccgtcgcc acggccccgt tcgtggcctg gcatttcggc
     3001 cccgtgccgc tcggggggct cgtgacgaac gtggccgggc tgccgctgac ggccgtcgtc
     3061 gtaacgggcg gcatgctggg cctggcgctt tcgcccgtcg cgggcgccgc cgacgccgcc
     3121 gcgcgggcgc tgctcttcgt ggccgaggta ggtgcggacg ccctgggctg ggctcggatc
     3181 gtcgtaccgg tgggcctggt cggcaccgtc cttctggcca cggccctctc ctgcacgctg
     3241 gcccggtcgg cccgcggccg gcgagtggcg gcggcggcgg ccctggtcgt cgcggtactc
     3301 gggccccgcc cgggaacgga ccttctccgc gtcgtcttcc tggacgtcgg ccagggggac
     3361 gcggccgttc tgcaccttcc ggacggggga agcgcgctcg tcgatacggg aacgccggcg
     3421 ggctcggccc cgtcgcgcta cctgtcgcgg atggggatcg accgcgtcga gcacgtcgtc
     3481 atcacgcacg accacgccga ccacgcggga gggctcgcca ggacagggga gacggtttcg
     3541 ttcggccgcg tcatcgggcc cgggcccgtc ggcgggcgac cggtgcacga ggtttcggcg
     3601 gcggacacgc tcgacttcgg gccgtcgctg cgcgtgtacg tcctcgcccc gttcggccgg
     3661 ggcgagggga acgaggcctc cctcgcgctc gccgtcgact tcggccgaac gaggctcctg
     3721 ttgatgggcg atgcggaaga ggcggcggag cgaaggatcg tcgaggcctg gcccgatctg
     3781 ctccggagcg acgtcgtgaa ggtcgggcac cacggctcgc gcacctcgag cacgcccctt
     3841 ctcgtgcgac gggccgcggc gcggggcacc ctggccgtcg tatccgtggg cgcgaggaac
     3901 cgctacgggc atccggcacc cggagtgctc cggcggtggg cggaggcagg ggccgtcgtc
     3961 ctgatgacgt ccggccgggg cgcggtctgc atcgagagtg acggccgcgg cgtccgggag
     4021 gcctgcccct aggagtcgag gaagagcggc acggggcagt ggtccacgag gttcttcgtc
     4081 gtcgacccga aggccattgc ccggaacgtg gatttcgagt gtgccccggc gaccacgaga
     4141 tcggcaccga actcttcgat ctgcgccagg agcgccgcgt cgatgtccga ttcgaggacg
     4201 gtcacccggt gcgcgtcgta gccgtgcgcc ttcaggtagt cgtcggcctg ctggagaaag
     4261 aactccgcct ccgtcggcgt gtcgcggtag atggcgacca gccggacatt cacctcgtga
     4321 ccgaagggat ccgcaagaac gaaggaccgc atggcccgcg ctgccgccgt gctcccgtcg
     4381 tacgcgatga gcaccttctt cacgtccctc acgtcctggt gtacgatcag cgtcgggcct
     4441 acggtgagcc gcacgatgcg cgccagcgtt gccgtggact ccttcggatg gctgtagaag
     4501 aaatgtggtt ctcggcccac gatcagcagg tcgtgaacct tcatgtcctc cacgatgcgc
     4561 tcgaaaggca ctccctcctg gacgagctcg gtgaaccgca cgcccgaatc gcggaccagg
     4621 cgttcgaact catccaggag ttcctgcgcc ttcgaccgcg cgtccgccgt cagctggtcg
     4681 cggagcttct cgacgtagta catgctcccg atgccgccac cacgcgacga cgattcgatg
     4741 ctccccatgt ccaccaccgc gaggccggtg atctccgcgt catggcggcg ggcgatgtcc
     4801 agcgcgaagc gcgtcgcgac cggcgtatcc gaatcgggat cgagcgcgac aagaatcttc
     4861 tttaccatcg tcttccggcg gctgcactgc tctcgaaagg taaccgtgac cacgtccggt
     4921 cgccaccgtc cggcatgcaa ccgcgtgcac ttctccacca atccacaccg ctcttatctc
     4981 ctcgaagaga agaatgaatt ggaatcggag tagtagtcag tcgcgtgcat atgtggacga
     5041 ggaaggaatc cacgccggct gcgtcggagc gtggattctt ccggggattc cgtggcgcgc
     5101 gcgacgggcc atcgcggggc cgaggagcgg gatggaagga gggtttccac atctccacga
     5161 gttatgcccc ggcgcgcacc ggccgggcgc ctccttttcc acggaaccgt ttcgtgtgga
     5221 tcgtccgtgg gaacgcgccc gaaaaaaacc ggtctttccg cgacgctccg gaacggcgtg
     5281 gataacggag ccgtcatcca cagtcggcgc ggccttccaa caaccggtcc acgaccggtt
     5341 ctacggacgg ccgaggagcg cgaacatctc gcgcttgtag gcctcgacgc ccggctggtc
     5401 gaacggattc acgcccagga gatagcccga caccgacacg gcgtgctcga agaagtacag
     5461 gagtccaccg accgacgtgg gatcgatcga cgggaggcgg atcacctgga ccggcacgcc
     5521 gcccgcgacg tgggcgcgga tcgttccctc ggcggccttc cggttcacga gggacagcgt
     5581 gcggcccgcg acgaagtcca ggccgtcgtc cgcgtcggga gaaccgggga tcacaaggcc
     5641 cccgccgtcc tcgtccggca gcaggaaggt ctccgcgacg ttgcgccggc cgtcctgaac
     5701 gtactgtccc accgagtgca ggtccgtcga gtactggacc gggacgggaa acagcccctt
     5761 gtgctccttg ccctcgctct cgccgaagag ctgctgccac caggcgccga ggcccgaaag
     5821 gcggggctcg aagacggcca gaagctcggt cgtgaagccg gcttcgtgca ggaggaaccg
     5881 cgtggcggcg taatccagcg ccgggttggc gtcgggcgaa gcgagggagc gccgggcgtc
     5941 gacggccccg tagaagagcg cgcggatgtc gacgccggcg acggcgatcg gcagcagtcc
     6001 gacgggcgaa agcacggaga atcggccccc gacgtcgggc ggaatgacgt accggcggta
     6061 tccggccgag gcgcgaaggc ggttcagggc gcccttctcc ggatccgtgg tcacgacgat
     6121 gcgccgcgcg gcgtcctcga accgccggcc catccattcg cggacgaccc ggaaagcgat
     6181 ggccggctcg agcgtggttc cgctcttgga caccacgttc acgtacacgg agcggccctc
     6241 gagatgcgcg aggagctcgc gcaggtaggc cccgctcatg tggtgcccgg cgaagaggat
     6301 ctcgggcccg ccgtgtccgg gagtcgggaa gtagggcgtg agggcggaga tggccgcgcg
     6361 ggccccgagg taggagccgc cgatgccgat gcacaggaac acgtcggcgc gggaacggat
     6421 ctcggcggcc aggtcgtcga tgtcgccgag gagcgcgtcg ttcggttcgc cgaggagcgc
     6481 gcgccacccc aggaagtcgg agcccgcgcc cgagccggag agcagcgtct cgtgcgcccg
     6541 gaggacgcgg ggatgcaggg cccggaaggc gccgtcgtcc agaaacggct cgacgccgga
     6601 gcggtcaagg tgtatcatgg ctcggtggcg cgtggcgccg tgggacggtc acaaactact
     6661 gcccggggga agcgcatgcg aacgttcgtg gaggtcgggg aagcccgcga aatcgtcctg
     6721 tcgacggccg cccttctgcc cgtgacgcac gtcccgctcg cggaggccgt cgggcggacc
     6781 ctggcgggcg acgtggtggc ggacatggac gtgccgccgt tcgacaactc ggcgatggac
     6841 gggtatgccg tccgtgccgc ggacgcagcc gcgcccgggt cggttctgcg cctttcgggc
     6901 ggcgtccggg ccggcgagtg gccatccgga gcggtcgatc ccggaatgtg ctggggcatc
     6961 acgacgggcg ctccgctgcc ggccggcgcg gacgcggtcg tgccggtcga gcaggcggag
     7021 gcgctcggcg acggcaggat ccggctcgcc gccgcgacgg aggccggacg ccacgtccgg
     7081 cgcgcgggtc gcgacgtcgc ggcggggcag gtggtggtca ccgacggggc cgcgatcacg
     7141 cccgccgtgg ccggtatact ggccatggtg ggcatgactc gggtgccggt ccgccgcccc
     7201 cccgtggtgg ccgtcgtggc gaccggggac gagctggtgc cgccggaccg tcggccgggg
     7261 ccgggacaga tccggaactc gaacggcccc gccctcgcgg cccaggccgt gtccgccgga
     7321 gccgtagtcc tcgccccgat gcacgcccgc gacgacgccg aggccacccg ggaggtcctg
     7381 cggcgggcgc tggaggcgga cgtcctggtg ttctcggggg gcgtatcgat ggggtctcgg
     7441 gacatcgtgc gcgacgtcct cggggagggc gggacggagt ggctcttctg gaaggtgcgg
     7501 cagcgccccg ggaagccgct cgcctgcggg cggctcggcg cccgtccggt gttcggactg
     7561 ccgggcaacc ccgtatcctc gtccgtgtgc ttcgaccagt acgtccggcc ggcccttctc
     7621 cggatgcagg gacgggagcc ggtgttccgg ccccggtacc gggccgttct cgaggaggag
     7681 ctgccgacgg cgccgggtct gcacacgttc gcccgcggcg tggcgttcgc cggggaaggc
     7741 ccggagctcc gcgtccgccc cacgggcccc cagggctcga acctgttcac gagcgtactg
     7801 cgcgcgaact gcctggttca cctgccggct tcccgggacg tcgcgcccgc ggggtccacg
     7861 gtcgagatcg agtggctcgc gtggtgagcg tcaggccggc ctgcaggtgg agatgccgaa
     7921 cgggagatac gccgggcagg tgcccacgat gctggtgagg atcaggacga cggccaggat
     7981 gacgagtatg gtggcgagcg tcccctgcac gacgccgagg aagaccagga gggcgatgac
     8041 gacggcagcg acggtgcgta cggcgcggtc gaccgcgccc atgttctttt tcatgggttc
     8101 ctgctcgagt agtgcgggcg cggcagtata gtcaacgatc gtccgtggag ccacttccgc
     8161 gagagagcgt ccggccccgc tgcggggccg tccgtccgac cggaatataa tgcgccgatc
     8221 ccggctcgcg tgaatccata caggtgtcat cccgtaatga caggtgttcc gcatggagtc
     8281 agcacgatga ataccgaaaa tatcccgttg cggagcggaa ggccgcgtca cgagcaggtc
     8341 agcacgtggc tgagggatcg gatcgagacg ggccgctacg gcccggacga ccagctcccc
     8401 tcggagagcc ggctcgtgga ccggttcggc gtgagccgga tcaccgtgcg cagggcgttg
     8461 cagacgctcg agagcgaggg tctgatctac cgtcggcagg gcctaggctc gttcgtgcgc
     8521 ggggagcgag tgcgccacgg gctggtccgg ctgaccgatt tcgccgagga catggaattg
     8581 gccggactcc acgcgtcgtc ccgggtcgag aaccgggcga tcgagccggc cacggcccgg
     8641 atcgcggcgg ccctgggcgt cgaggaagga gcgccggtcc ttcggctgga ccgggttcgc
     8701 ctggggaacg gagaccccat cgccttcgac cggacctggc tgcccctctt ctatgcgcag
     8761 ctgctcgacg gccgcgacct gggcagcgag accatctacc agattctgga gcgggacggc
     8821 ggcatcacga tggaacgggg ccgcttccgg ttcgaggccg ttaacgcgcc gtccgacgtg
     8881 gggcgcgccc tgggcgtgcc ctgggggcgg gcgctgctgc tggtcgaacg cgtgagctac
     8941 gcggagcggg gtcggtgcgt ctatttccag cagcgcttct accggtgcga ccgggtggcc
     9001 tacgagctcg acctcgagcg cgaatccggc gcgacgggcc ccgggctgcc gatccgggag
     9061 ttcgagcccg tgttcaaggc gacggaggac gcggggtgac ccggcggcgg gaatgacgtc
     9121 ccggcggggc gtaacttccg ggatccaccg gaaaacgaca cccgcactcc atgcgtcgcg
     9181 ccctgctcgt cctggtcgtc tcgaccctcg cccttccggc ctccgggcag gatctcaact
     9241 cgccttccgg cttcctgggc tacgaactgg gcacccgctt cacgccccac caccgggtcc
     9301 tggaatacgt ggaccacgtg gcggccgcat cggaccgggt gcgcgtcgag cggtacggcg
     9361 aatcggtcga aggtcgtccc ctggccctcg ctttcgtggc gagcccggag aacctcg
//
LOCUS       DBNHQZ010000301         7844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3477031, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000301 DBNHQZ010000000
VERSION     DBNHQZ010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3477031"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="AC16YU_08075"
     CDS             <1..817
                     /locus_tag="AC16YU_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR6065220.1"
                     /translation="MQRNRTRFHAAADGVRVGKISGAVGTFAHVPTDVERITCERLGL
                     EPAPISTQVLQRDRHAHYLGVLALVGATLEKIAVEIRGLQKSEVREVEEAFRKGQKGS
                     SSMPHKRNPVGSENVTGCARLLRGYMVAAYENVALWHERDISHSSVERVILPDATTVL
                     HYALDRMARILDDLVVYPDAMRRNLDRTFGLYNSQRLLNALIDRGLSREAAYDLVQPR
                     AMQAWEEGRSFLEIVLAAPEVTEHLSEGEIRDVFDPEFHLRRVDEIFGRVGLD"
     gene            complement(818..1324)
                     /locus_tag="AC16YU_08080"
     CDS             complement(818..1324)
                     /locus_tag="AC16YU_08080"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6065221.1"
                     /translation="MKPEIVIIAAVGARDRVIGRGLRLPWHIPEDLKRFKRLTSGHPL
                     VMGRRTFESLVVQFGRPLPNRRNIVVSGSGRFDAPDGVEVYESLDEALAAAADADRVF
                     IGGGASLYEHFLPIADRMELTLVDGEHRGDVFFPPYEHLVGPVYEVRDVEEHDGFRFV
                     TYVRRAGA"
     gene            complement(1321..2115)
                     /locus_tag="AC16YU_08085"
     CDS             complement(1321..2115)
                     /locus_tag="AC16YU_08085"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6065222.1"
                     /translation="MRPYLDLLRLVRETGLRKEDRTGTGTLSVFGYQMRFDLQAGFPL
                     VTTKKVFFKGLAVEMLWFLRGGTNVRWMQERGVHIWDPWADEEGNLGPVYGKQWVAWE
                     SRDGTPINQIARVVEQIRTRPDSRRHVVNAWNVADLERMALPPCHMFFQFYVGDGRLS
                     CHLYVRSNDLFLGAPFNIAEYALLTHMMAQQCDLEPGELVYTIGDAHVYLNHLDQVDE
                     QLARDPLPLPRLELVRRPGSIFEYEYEDFALLDYRHHPAIKAPVAV"
     gene            2222..2512
                     /locus_tag="AC16YU_08090"
     CDS             2222..2512
                     /locus_tag="AC16YU_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065223.1"
                     /translation="MLQPGVGSKKMEPNLHQKQGINHLNRVLAYAKFVTENGRATVHL
                     TSEDWHVVADTLFHMETPAEMLPEAIESFRMTGNGGAIELRTPGIVIEMLVI"
     gene            2552..3622
                     /locus_tag="AC16YU_08095"
     CDS             2552..3622
                     /locus_tag="AC16YU_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR6065224.1"
                     /translation="MTLLIIYVVVALGVSFMCSIMEAVLLSVSPSFAAQAASDGAPYG
                     RRLKALKDEIDRPLAAILSLNTIAHTVGAAGAGAQAAIVFGSAYLGLASAVLTLLILV
                     LSEIIPKTVGALYWRPLIPIVVRTVGPVIRMMWPLVKMAEWITRLIARNRGHVTLRRD
                     EFAALTRVGATEGVLRDDESRMMRSLLRFGELRARDVMTPRTVVSHVEAGTTVGDLFR
                     QRPEMRFSRLPVTVNGMDQTIGFVLRSDLLQAAAMGRGDAAVDEFLRPLPAVPDGTSL
                     RDLFDRLLAGNTHMALVVDEFGGAEGLVTVEDVIETLLGLEIVDEADTVRDMQVLARE
                     RWAARAREMGLLDADSDRPEPG"
     gene            complement(3633..4016)
                     /gene="apaG"
                     /locus_tag="AC16YU_08100"
     CDS             complement(3633..4016)
                     /gene="apaG"
                     /locus_tag="AC16YU_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR6065225.1"
                     /translation="MIPYVAITDDIAVTVRPVYLDGQSNVLGRRFVFGYFVRIENRGA
                     DEVQLLRRHWTISDSEGRVQEVEGEGVVGHTPVIAPGEAHEYNSFCTIETFEGAMEGT
                     YLMERARGVRFRVAIPRFDLRAAAN"
     gene            complement(4073..4915)
                     /locus_tag="AC16YU_08105"
     CDS             complement(4073..4915)
                     /locus_tag="AC16YU_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein"
                     /protein_id="HIR6065226.1"
                     /translation="MQRTFEDLFPDPRPVIGVIHTGPSPGVPGFICVESCVERAVAEA
                     EAYVQGGVDGIIVENMHDFPCVHEREMGPEIAAFMTRIGRATKRRAGRLPVGLHVLFQ
                     ANRTALAAALASGCDFVRAEGWTHAHISDKGVAHASAGDTIRYRHMIGASHLPIFTDI
                     RKKHASHSWTSDLGIDDIAFGMALHRSDAVIVTGSHTGTAPSVDDLIAVRHATDLPVL
                     VGSGITVENLDEIAPLADGFIVGSAMKENGHWDAPVSEARVQAIVEAVRRLRAEPEEA
                     ASRS"
     gene            5247..7109
                     /locus_tag="AC16YU_08110"
     CDS             5247..7109
                     /locus_tag="AC16YU_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6065227.1"
                     /translation="MAQKTVESLPEATILFAGDSGDGMQVTGSQFTLATAYAMNDLAT
                     LPDFPAEIRAPAGTTYGVSGFQLHFGSVDIRTPGDEVDLLVAMNPAALAVNLDRVRRG
                     GTIIVNVNAFDRRSLDLAGLAENPLENGSLEGYQVIPVELTRLTREALSDSGLNQKEI
                     DRSKNMFSLGLALWLYSRPIEPAERWISRKFARKEAILEANLHLLRKGYHYGETTEQF
                     AVRYEVTPAKLPPGQYRAIQGVTAMALGLVAASDRSGLPLFYGSYPITPASDLLHALS
                     RYKNHGVMTFQAEDEIAAVGSAIGAAFGGSLAVTGTSGPGIALKTEAIGLAVIMELPL
                     IIVNMQRGGPSTGLPTKTEQSDLLQAMYGRNGDTPLPIVAASTPGDCFESAYEAARIA
                     VRHMTPVMLLADGYLANGSEPWLIPDPSSLPAIDVEFATEPNRTVDGEAVFLPYVRDP
                     ETLARPWATPGTAGLEHRIGGLEKQHETGNVSYDADNHELMTRLRAEKVESIARSLPP
                     LPVNGDAEGDLLIVGWGSTRGAIEAAVERLRARGRRVSSVHLRHVNPLPSDLGDIVSR
                     FRHVLVPELNRGQLVRILRDRYLLPFIAFNKVQGRPFRAGELAEHVESILDGSK"
     gene            7115..>7844
                     /locus_tag="AC16YU_08115"
     CDS             7115..>7844
                     /locus_tag="AC16YU_08115"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6065228.1"
                     /translation="MSEQSESPRSGGRKPTLPPGPGRKPAAPPGARPPAAAPPRRPGS
                     AAGDGAAVLTRADFTSDQDVRWCPGCGDYAILATVQRLMPELGVPREKTVFISGIGCS
                     SRFPYYMNTYGMHSIHGRAPTIATGLKASRPDLDVWIVTGDGDGLSIGGNHLIHLMRR
                     NVNVQLLLFNNQIYGLTKGQYSPTSEKGKITKSTPFGSIDHPFNPIALVLGADASFVG
                     RTMDRDPKHMKDMLQRAHDHRGTAF"
BASE COUNT         1236 a         2714 c         2706 g         1188 t
ORIGIN      
        1 gatgcagcgc aaccgcaccc ggttccacgc cgcggccgat ggcgtccgcg tgggcaagat
       61 ctcgggagcc gtcgggacct tcgcgcacgt tccgaccgac gtggagcgga tcacgtgcga
      121 gcgcctcggg ctcgagccgg cgccgatctc gacccaggtg ctgcagcgcg accggcacgc
      181 ccactacctg ggagtcctgg cgctcgtcgg tgccacgctc gagaagatcg cggtcgagat
      241 ccgagggctg cagaagagcg aggtgcgcga ggtggaggag gccttccgca aggggcagaa
      301 gggctcgtcc tccatgccac acaagcgcaa cccggtgggc tcggagaatg tcaccggctg
      361 cgcgcggctg ctgcgcggct acatggtagc ggcctacgag aacgtggcgc tgtggcacga
      421 gcgggacatc tcgcactcct ccgtggagcg ggtcatcctg ccggatgcga cgaccgtgct
      481 ccactatgcg ctggaccgga tggcccgcat cctcgacgac ctcgtggtct atcccgacgc
      541 gatgcggcgc aacctggacc ggaccttcgg cctgtacaac agccagcggc tcctgaacgc
      601 gctgatcgat cgcggcctga gccgcgaggc cgcctacgat ctcgtgcagc cgcgcgccat
      661 gcaggcgtgg gaggagggac gctcgttcct cgagatcgtc ctcgccgccc ccgaggtgac
      721 cgagcacctc tccgagggcg agatccgcga cgtgttcgac ccggaattcc acctccggcg
      781 ggtggacgag atcttcgggc gcgtcgggct cgactgatta cgcgccggcc cgccgcacgt
      841 acgtcacgaa gcggaagccg tcgtgctcct cgacatcgcg gacctcgtag acgggtccga
      901 ccaggtgctc gtacggagga aagaacacgt cgccccggtg ctcgccgtcc accagcgtga
      961 gctccatgcg gtcggcgatc ggcaggaagt gctcgtacag gctggccccg cccccgatga
     1021 agacgcggtc ggcgtcggcg gcggccgcga gcgcctcgtc gagcgactcg tacacctcga
     1081 ccccgtccgg cgcgtcgaac cgcccggagc ccgacacgac gatgttgcgg cggttcggga
     1141 gcggccgccc gaactggacg acgagcgact cgaaagtgcg cctccccatg actagtggat
     1201 gaccgctcgt cagccgcttg aaccgcttca ggtcctcggg aatgtgccag ggaagccgca
     1261 gcccgcggcc gatgacgcgg tcgcgcgcgc cgaccgccgc gatgatcacg atctcgggct
     1321 tcataccgcc acgggtgcct tgatggcggg atggtggcgg tagtcaagaa gcgcgaagtc
     1381 ctcgtactcg tactcgaaga tcgatccggg acggcgcacg agctccaggc gcgggagcgg
     1441 cagggggtcg cgcgcgagct gctcgtccac ctggtccagg tggttcaggt aaacgtgcgc
     1501 gtcgccgatc gtgtagacga gctccccggg ctccaggtcg cactgctgcg ccatcatgtg
     1561 cgtgagcagc gcgtattcgg cgatgttgaa cggtgcgccc aggaaaaggt cgttcgagcg
     1621 cacgtacagg tggcacgaga gccggccgtc cccgacgtag aactggaaga acatgtggca
     1681 cggcggcagc gccatgcgct ccaggtccgc cacgttccac gcgttcacga cgtggcgcct
     1741 cgagtcgggc cgtgtccgga tctgctccac gacgcgggcg atctggttga tgggggtccc
     1801 gtcccgcgac tcccaggcca cccattgctt gccgtacacg ggccccaggt tgccctcctc
     1861 gtccgcccac gggtcccaga tgtgcacgcc gcgctcctgc atccaccgga cgttggtccc
     1921 gccgcgaagg aaccagagca tttcgaccgc aaggcccttg aagaacacct tcttggtcgt
     1981 cacgagcgga aacccggcct gcaggtcgaa gcgcatctgg tagccgaata cgctcaacgt
     2041 gccggtgccg gtgcggtcct ccttgcggag gccggtctcg cgaacgaggc ggagcagatc
     2101 gagatacggg cgcatggtcg gatcctgcgg cttcctggcg gattaacgcc tttctccccc
     2161 ggggcgggcg cgaccgacgc ggatcggcgg cccgccgatt cgacaatcaa ccgatggacc
     2221 aatgctgcaa cccggcgtcg gatcgaagaa gatggagccg aacctccacc aaaagcaagg
     2281 gatcaaccac ctgaaccgcg tgctcgcgta cgcgaagttc gtgaccgaga acggccgggc
     2341 gacggtgcac ctgacgtcgg aggactggca cgtggtcgcc gataccctgt tccacatgga
     2401 aacgcccgcg gagatgcttc ccgaggcgat agagtccttc cggatgaccg gaaacggcgg
     2461 ggcgatcgag ctccggacgc cgggcatcgt catcgagatg ctggtgatct agtcggctca
     2521 cccaaccgtc gagtaccggc ggcacgtctt catgaccctg ctgatcatct atgtagtcgt
     2581 ggcacttggc gtctcgttca tgtgctcgat catggaggcg gtcctgctca gcgtatctcc
     2641 gtcgttcgcg gcccaggcgg cctcggacgg cgccccgtac gggcgacggc tgaaggccct
     2701 caaggacgag atcgaccgac cgctggccgc gatcctgagc ctgaacacca tcgcccacac
     2761 ggtgggggcc gcgggtgccg gagcgcaggc ggccattgtg ttcgggagcg cgtacctggg
     2821 cctggcgtcg gccgtgctga cgcttctcat cctggtactt tcggagatca tcccgaaaac
     2881 agtgggcgcg ctctactgga ggccgctcat cccgatcgtg gtccggaccg tcggccccgt
     2941 catccggatg atgtggccgc tggtcaagat ggcggagtgg atcacgcggc tgatcgcccg
     3001 caaccgcgga catgtgacgc ttcgccgcga cgagttcgcc gcgctgacga gggtcggtgc
     3061 gacggagggg gtcctgcgcg acgacgagtc caggatgatg cgcagcctgc tccggttcgg
     3121 ggaactgagg gcccgggacg tcatgacccc gagaaccgtg gtttcccacg tggaggccgg
     3181 gaccaccgtg ggagacctgt tccggcagcg tccggaaatg cggttctcgc ggctgcccgt
     3241 taccgtgaac gggatggatc agacgatcgg cttcgtgcta cggtccgacc tcctgcaggc
     3301 agccgcgatg gggcggggcg atgccgcggt cgacgaattc ctgcggccgc ttcccgcggt
     3361 ccccgacggt acgtcgttgc gggacctgtt cgaccggctt cttgccggca acacccacat
     3421 ggcactggtc gtggacgaat tcgggggtgc ggaggggctc gtcaccgtgg aggacgtcat
     3481 cgagacgctg ctgggcctcg aaatcgtgga cgaagcggac accgtgcggg acatgcaggt
     3541 gctggcccgc gaacgatggg ccgccagggc acgggagatg ggcctgctgg acgcggattc
     3601 ggaccgcccg gagccggggt gaagccggcc gctcagttgg ctgccgcgcg caggtcgaac
     3661 cgggggatgg cgacgcggaa ccgaacgccg cgggcccgct ccatcaggta ggtgccctcc
     3721 attgcgccct cgaaggtctc gatggtgcag aaggaattgt actcgtgcgc ctcgcccggc
     3781 gcgatgacgg gcgtatggcc caccacgccc tcgccctcga cttcctggac gcgtccctcg
     3841 gaatcgctga tcgtccagtg ccgcctgagg agctgcacct cgtccgcgcc ccggttctcg
     3901 atccggacga agtacccgaa cacgaacctg cgcccgagca cgttcgactg cccgtccagg
     3961 tacaccgggc ggaccgtgac ggcgatgtcg tcggtgatgg ctacgtaggg gatcatcgtt
     4021 cagcgcgtgt ggatgaccta acgtcgcgcg ccgcggcggg atccggacgg actcagctcc
     4081 gggaggcggc ctcctcgggc tccgcgcgca gccgccggac ggcctcgacg atcgcctgga
     4141 cccgcgcctc gctcaccggc gcgtcccagt gtccattctc cttcatggcg ctgccgacga
     4201 tgaaaccgtc ggcgagtggc gcgatctcgt cgaggttctc gaccgtgatc ccgcttccga
     4261 ccaggacggg gaggtcggtc gcgtggcgca ccgcgatcag gtcgtcgacg ctcggcgccg
     4321 tgcccgtgtg cgatccggtc acgatcacgg cgtcggaccg gtgcagggcc atgccgaagg
     4381 cgatgtcgtc gatcccgagg tccgacgtcc aggaatgcga tgcgtgcttc ttgcggatgt
     4441 ccgtgaatat gggaaggtgc gaggcgccga tcatgtgccg gtagcggatc gtgtcgccag
     4501 cggacgcgtg ggccacgccc ttgtccgaga tgtgggcatg ggtccagccc tcggcccgca
     4561 cgaaatcgca gcccgaggcc agtgccgctg cgagggctgt ccggttcgcc tggaacagca
     4621 cgtgcaggcc cacgggaagg cgtccggccc tgcgtttggt cgcgcggccg atccgcgtca
     4681 tgaaggcggc gatttccggc cccatctcgc gctcgtgcac gcaggggaaa tcgtgcatgt
     4741 tctcgacgat gatgccgtcc acaccgccct gcacgtacgc ttccgcctcg gcgacagccc
     4801 gctccacgca gctctccacg cagatgaagc cgggcacccc ggggctgggt cccgtgtgga
     4861 tgacgccgat gacgggacgc gggtcgggaa acaggtcctc gaaggtgcgc tgcatcggtg
     4921 gtcctggtcg gtggctgcgt cgcctaaacc gatgcggcga gcaagttcct tgtccgcccg
     4981 cgggggcccg cgccgacgcc gggcgcggcg tcccgggcag gcccgccggt gccgtgtgcg
     5041 gcgccccggg cagaccggcc gcgcgcacgg aatggcgccg ccatgctgcc cgtccgggcg
     5101 aggcttcgcg tggcgtcgta acatggacgg aatccggccg acgtcccgcg cgttcgcggg
     5161 gggtttccct acacgatcac ggattcgcgc gcagtcgcga tctccatata gcgcggcggc
     5221 gccctgcgcg cccccgcccg ctcatcatgg cgcagaaaac cgtcgagtcg ctgcccgagg
     5281 cgacgatcct cttcgcgggc gactcgggcg acggcatgca ggtcacggga tcccagttca
     5341 cgctggcgac cgcctacgcg atgaacgacc tcgccaccct gccggacttt ccggccgaga
     5401 tccgggctcc cgccggcacg acgtacggcg tcagcggctt ccagctccac ttcgggtcgg
     5461 tggacatccg gacgccgggc gacgaggtcg acctcctggt cgcgatgaac ccggccgcgc
     5521 tcgcggtgaa cctggatcgg gtgcgccgcg gcgggacgat catcgtgaac gtgaacgcct
     5581 tcgaccgccg gagcctggac ctggcgggcc tcgcggagaa tccgctcgag aacggctcgc
     5641 tggaaggcta ccaggtcatt cccgtggagc tcacgcggct cacgcgcgag gcactctcgg
     5701 actccggact gaaccagaag gagatcgacc gctccaagaa catgttctcg ctcgggctgg
     5761 ccctctggct gtactcccgc ccgatcgagc cggccgagcg ctggatctcc cgcaagttcg
     5821 cccggaagga ggccatcctg gaggccaacc tgcacctcct gcgcaagggc taccattacg
     5881 gggagacgac agagcagttc gccgtgcgct acgaggtgac cccggcgaag ctgccgcccg
     5941 ggcagtaccg ggcgatccag ggggtgacgg ccatggcgct gggcctcgtc gcggcgtcgg
     6001 accggtccgg actgccgctc ttctacggct cgtacccgat cacgccggcc tcggacctgc
     6061 tgcatgccct cagccgctac aagaaccacg gcgtcatgac cttccaggcc gaggacgaga
     6121 tcgcggccgt cgggtccgcg atcggtgcgg cgttcggagg aagcctcgcg gtgaccggca
     6181 cgagcggtcc gggcatcgcg ctcaagaccg aggccatcgg cctggcggtc atcatggagc
     6241 tgccgctcat catcgtgaac atgcagcgcg gcggcccgtc caccgggctc ccgaccaaga
     6301 ccgagcagag cgacctgctc caggccatgt acggccggaa cggcgacacg cccctgccca
     6361 tcgtcgcggc gagcacgccg ggcgactgct tcgagtccgc gtacgaggcg gcgcgcatcg
     6421 cggtgcggca catgacgccg gtcatgctgc tcgccgacgg ctacctcgcg aacggcagcg
     6481 agccgtggct catcccggac ccgtcatcgc tgcccgcgat cgacgtcgag ttcgcgacgg
     6541 agccgaaccg gacggtggac ggcgaggccg tcttccttcc gtacgtgcgg gatccggaaa
     6601 ccctggcgcg cccgtgggcc acgccgggaa cggccgggct cgagcaccgg atcggcgggc
     6661 tcgagaagca gcacgagacg ggcaacgtct cgtacgacgc ggacaaccac gagctcatga
     6721 cgcgcctccg ggcggagaag gtggagagca tcgcccgctc gctgccgccc cttccggtga
     6781 acggggatgc cgagggcgac ctcctgatcg tgggctgggg ctctacccgg ggcgcgatcg
     6841 aggccgccgt ggagcgcctg cgcgcccgcg gcaggcgcgt ctcgtcggtg cacctccgcc
     6901 acgtgaatcc cctgccgagc gacctcgggg acatcgtgtc gcgcttccgg cacgtcctcg
     6961 tgcccgagct caaccggggg cagctggtgc gcatcctgcg cgaccgctac ctgctgccct
     7021 tcatcgcatt caacaaggtc cagggccggc ccttccgtgc gggcgagctg gccgagcacg
     7081 tcgagtcgat cctcgacggg agcaagtgag cgccatgtcc gagcagagcg aatcccccag
     7141 gtccggaggc cgcaagccga cccttccacc gggtccgggc cgcaagccgg ccgcgccgcc
     7201 cggagcgcgc ccgccggctg ccgcgccgcc gcgccgcccc gggtcggcgg cgggcgacgg
     7261 ggccgcggtg ctgacgcgcg ccgacttcac ctccgaccag gacgtccgct ggtgtccggg
     7321 gtgcggcgat tacgccatcc tcgccaccgt gcagcgcctg atgccggagc tcggcgtgcc
     7381 ccgcgagaag accgtcttca tcagcggcat cgggtgctcg agccgcttcc cgtactacat
     7441 gaacacgtac gggatgcact ccatccacgg gcgtgccccg acgatcgcca cggggctcaa
     7501 ggccagccgc ccggacctgg acgtatggat cgttaccggc gacggcgacg ggctgtcgat
     7561 cggcggcaac cacctgatcc acctgatgcg gcgcaacgtg aacgtgcagc tgctgctctt
     7621 caacaaccag atctacggcc tgaccaaggg gcagtacagc cccacctccg agaagggtaa
     7681 gatcacgaag tccacgccct tcgggtcgat cgaccatccg ttcaacccga tcgctctcgt
     7741 gctgggcgcc gacgcctcgt tcgtgggccg caccatggac cgcgacccga agcacatgaa
     7801 ggacatgctg cagcgggccc acgaccatcg gggcacggcc ttcc
//
LOCUS       DBNHQZ010000302         4051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3069513, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000302 DBNHQZ010000000
VERSION     DBNHQZ010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3069513"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="AC16YU_08120"
     CDS             <1..1422
                     /locus_tag="AC16YU_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065229.1"
                     /translation="SEIRLELYVAEGNTSTGTILFDRLSPYGFQETDFEAPEAPTGLA
                     ADPSVFSNIVSWTDVPGESGETYTLYASQAPITDVAAPGVMLVKTGIAEGTEIFTHKL
                     YHPVVDYDLSLYYALSATDAVGNVGPAAALATPVTTTAKGMSTISIGTPPSFAADGDL
                     SEWAGLTPFHMEFGTEFGFDGMATGLTPSAADLSMDFWLAMDEENLYFAAEVTDDVVD
                     SWTGTGNHYQYDEIQLYIGLFDRNTTYSSGRRGWPVPDYEISMVETRGGFSSLTWPAG
                     SDPAPGDGSGFYSWVETAGGYNVEFKIPFTVMQRDGQPSPFVPVEGMRLPLDISLHDD
                     DTDDGQATQDFILTFSSTNENLAYQDVSRWLYTWIGEKYSVVGVERVDTDVPQVFNLH
                     PNYPNPFNPTTVFRYDLPEAGPVTLRVFNMLGQEVRTLIDGMQAAGTYEVQFDAQNLA
                     SGMYLYQLRSGRYTQTRKMMLVR"
     gene            1547..3637
                     /locus_tag="AC16YU_08125"
     CDS             1547..3637
                     /locus_tag="AC16YU_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HIR6065230.1"
                     /translation="MSPRASALHWLATVLAAAVLLAAPAERAMGQGVTESIRANQVGY
                     WTDGDKRAVVVDSPTLTFEIRSPDLATLAYTGTLGGRQRWIAASDQEYARVAEFSDFE
                     TEGEWVVHVPGLGSSHPFRVGRRVHEGLTRAVLKAFWFARASTALPAAWAGPWAREAG
                     HPDTNVLVHPSAASASRPAGSMIAAPRGWYDAGDYNKYVVNSGITTATLLMLYERFPT
                     YFDALRLDIPESDGGLPDLLDEVLWNLRWMMAMQDPEDGGVYHKLTTPQFEAAVMPRD
                     AVQPRYVVQKSSSATLNFAAVAAIGARVFAGFPPMRALSDSLLEAASRAWVWAHYRES
                     DLLYDQGAMNAAFDPDIQTGQYGDGDLYDEYRWASTELFITTGADSLLVMYNPIPASM
                     PSIPSWNDVTALGLWSLARHRAQVAGSVDTALLRSRILERAEDLVRRDSLSAYGFVFG
                     GTSSDYVWGSNSVAANQANMLLVAWDLKRDPRYLRAATGNLDYLLGRNAIGTSFVTGF
                     GERSPMHPHHRPSQADGVVPPVPGMLVGGVNPGRQDGCTYPFPANQPARSWVDAWCSY
                     ASNEVAINWQASLAFLAAGLEAILSPDGLPASAAAEPLPAVRPRGFEVEAWPNPARGA
                     ATVSFTLARDTTVKGGVYDLLGRRVLLIEDGGRLPAGRHERQVQAGSLAAGVYLVRFE
                     ADGAFIARPLIVTR"
     gene            3752..>4051
                     /locus_tag="AC16YU_08130"
     CDS             3752..>4051
                     /locus_tag="AC16YU_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR6065231.1"
                     /translation="MSATRVWRLTLWIVLVGAVIAASALILTRDGEDPDDAPVAVVDG
                     VPITRARFETWYIERLIATGRADTPSRRYSELDNLVQIRLLADEARRRGLDTGGEF"
BASE COUNT          627 a         1388 c         1379 g          657 t
ORIGIN      
        1 tccgagatcc ggctggagct ctacgtggcc gagggcaaca cctcgacggg caccatcctg
       61 ttcgaccgcc tctcgccata cggcttccag gagacggact tcgaggctcc cgaggcgccg
      121 accggcctgg ccgcggatcc gtccgtcttc tcgaacatcg tttcctggac cgacgtcccg
      181 ggcgaaagtg gcgagaccta cacgctgtac gcgagccagg cgccgatcac cgacgtcgcc
      241 gcgccgggcg tgatgctggt caagaccggg atcgccgagg gtacggagat cttcacgcac
      301 aagctgtacc acccggtcgt cgactacgac ctgagcctgt actacgcgct ctcggcaacg
      361 gatgccgtgg gcaacgtggg gccggcagcc gcgctcgcaa cgccggtcac gacgacggcc
      421 aaggggatgt cgaccatctc gatcggcacg ccgccaagct tcgccgccga cggtgacctg
      481 tccgagtggg ccggcctgac tcccttccac atggaattcg ggaccgagtt cggcttcgac
      541 ggcatggcca cggggctgac gccttcggcc gccgacctgt cgatggactt ctggctggcg
      601 atggacgagg agaacctcta cttcgcggcc gaagtgacag acgacgtggt ggattcgtgg
      661 accggcaccg gcaaccacta ccagtacgac gagatccagc tgtacatcgg tctcttcgat
      721 cggaacacga cgtactcgag cggccgcagg ggctggcccg tcccggacta cgagatcagc
      781 atggtggaga cccggggcgg cttctcgtcg ctcacctggc cggccggctc ggatccggcg
      841 ccgggtgacg gctcgggctt ctactcgtgg gtggagacgg cgggcggcta caatgtggag
      901 ttcaagattc cgttcaccgt catgcagcgg gacggccagc cgtctccgtt cgtgccggtc
      961 gaggggatgc ggctcccgct cgacatctcg ctccacgacg acgacaccga cgacggccag
     1021 gcgacacagg acttcatcct gacgttctcg tcgaccaacg agaacctggc gtaccaggac
     1081 gtttcccggt ggctgtacac gtggatcggc gagaagtact cggtggtggg cgtggagcgt
     1141 gtcgacacgg acgttccgca ggtcttcaac ctgcacccga actacccgaa cccgttcaac
     1201 ccgaccaccg ttttccggta tgacctgccg gaggcgggac cggtgaccct ccgggtgttc
     1261 aacatgctcg ggcaggaagt ccgtacgctc atcgacggca tgcaggccgc cggtacgtac
     1321 gaggtgcagt tcgacgcgca gaacctggcg tccggcatgt acctctacca gctgcgctcg
     1381 ggccggtaca cccagacccg gaagatgatg ctcgtccgct aggccgcgcg gcctaaccgg
     1441 gatccatcgt cccggcaccg tgacggtgcg gcagaggggg cgggtgccgc tgtgcccgcc
     1501 ccctctgtcg tgctgcgccc tttccgagcc ctgaaccccc acggccatgt caccacgcgc
     1561 ctcggcattg cattggctcg cgaccgtcct ggccgcggcc gtccttctcg ccgctccggc
     1621 ggaacgggcc atgggccagg gggtcacgga gtcgatccgt gcgaaccagg tcggctactg
     1681 gacggacggc gacaagcggg ccgtggtcgt ggactcgccc acgctcacct tcgaaatccg
     1741 atctcccgac ctcgccacgc tggcctatac cggcaccctc ggggggcggc agcgctggat
     1801 cgcggcgtcc gaccaggagt atgcccgcgt cgcggaattc tccgatttcg agaccgaggg
     1861 ggagtgggtc gtccacgtgc ccggcctggg cagttcgcac cccttccgag tcggtcggcg
     1921 agtccatgaa gggctgaccc gggccgtgct caaggcgttc tggttcgccc gggcctccac
     1981 cgcccttccg gcggcgtggg ccgggccatg ggcgcgggag gcaggacatc cggatacgaa
     2041 cgtcctggtc cacccttcgg ccgcatcggc gagccggccc gccggcagca tgatcgccgc
     2101 accgcgcggg tggtacgacg ccggagacta caacaagtac gtggtcaact ccgggatcac
     2161 gacggcgacg ctgctcatgc tgtacgagcg cttcccgacc tacttcgacg cgcttcgcct
     2221 ggacatcccc gaatcggacg gcgggcttcc cgacctgctc gacgaggtgc tctggaacct
     2281 gcggtggatg atggccatgc aggatccgga ggacggcggc gtgtaccaca agctgaccac
     2341 gccgcagttc gaggcggccg tgatgccgcg ggatgccgtg cagccacggt acgtggtcca
     2401 gaaatcctcg tcggcgacgc tcaacttcgc ggcggtggcg gctatcgggg cccgggtgtt
     2461 cgccggcttt ccgcctatga gggcgctttc ggattccctg ctcgaggcgg ccagccgggc
     2521 gtgggtctgg gcacattacc gggagtccga cctgctctac gaccagggcg caatgaacgc
     2581 ggccttcgac ccggacatcc agaccgggca gtacggtgac ggagacctgt acgacgagta
     2641 ccgctgggcg tcgaccgaac tgttcatcac gaccggtgcc gacagcctgc tggtcatgta
     2701 caatccgatt cccgcgtcga tgccgtcgat tccgagctgg aacgacgtga ctgcactcgg
     2761 gctctggagc ctggcccggc accgagccca ggtcgcaggc tcggtagaca ccgccctgct
     2821 ccggagccgg atcctggagc gcgccgagga cctggtccgc agggacagcc tttcggcgta
     2881 cggattcgtg ttcggcggca catcgtcgga ctacgtgtgg ggcagcaatt cggtcgccgc
     2941 gaaccaggcg aacatgctcc tggtggcctg ggacctcaag cgcgatccgc gctacctccg
     3001 ggcggccacc gggaacctgg actaccttct cggccgcaac gccatcggaa cgtcgttcgt
     3061 cacggggttc ggcgaacggt cgcccatgca cccgcaccac cgtccctcac aggcggacgg
     3121 cgtcgtgccc cccgtacccg gcatgctcgt cggaggcgtg aatcccggcc ggcaggacgg
     3181 gtgcacgtat ccgttcccgg cgaaccagcc ggcgcgttcc tgggtcgatg cctggtgcag
     3241 ctacgcttcg aacgaggtcg cgatcaactg gcaggcctcg ctcgctttcc tggcggccgg
     3301 cctggaggcg atcctgtcgc ccgacgggct gccggcctcg gctgcggcgg agccattgcc
     3361 cgccgtgcgg ccacggggct tcgaggtgga ggcctggccc aatccggcac ggggcgccgc
     3421 caccgtgtcc ttcacgctgg cgagggacac cacggtgaag ggcggcgtgt acgatctgct
     3481 cgggcggcgt gtcctcctga tcgaggatgg aggccggctg ccggcgggac gccacgaacg
     3541 ccaggtgcag gccggatccc tggcggccgg cgtctacctg gtccggttcg aggcggacgg
     3601 ggcgttcatc gcccggccct tgatcgtgac ccgatgagcg ggagcgtccg gtgatccgtc
     3661 gcggaagacg accgtcccgg tcgccgaggc gtggaggagc cgatccctgt cccgcgcgta
     3721 cgtcgaactc ggattcatcc atctcctgga catgtctgca acacgcgtct ggcgtctgac
     3781 cctttggatc gtgctcgtgg gggcggtcat cgccgcaagc gcgctgatcc tgacccgaga
     3841 cggggaggat ccggacgacg cgcccgtggc ggtcgtcgac ggggtgccga tcacccgggc
     3901 ccggttcgag acctggtaca tcgagcggct gatcgctacc ggccgcgcag atacgccatc
     3961 ccgtcggtac agtgagctcg acaacctggt ccagatccgg ctcctggccg acgaggcgcg
     4021 gcggcggggc ctcgacaccg gcggggaatt c
//
LOCUS       DBNHQZ010000303         6843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2934935, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000303 DBNHQZ010000000
VERSION     DBNHQZ010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2934935"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC16YU_08135"
     CDS             <1..822
                     /locus_tag="AC16YU_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065232.1"
                     /translation="FATLTVDEILAPRTTDDALDLVSGWDLLTLAAIEERKKLYVNQF
                     GCSEMDMVLSDDTGDLAAYYATGETAEFSGGVRVAVGDVNGDGTCTVALLVPAVQKIR
                     ASTQGHGDTEWRFEAYLPLENGRMDAAVSPPVALSLGSKATYDDDAGGGIGTSVLLDA
                     DGDGEVDITFPLGRTTVGTDPRREVPEDHALDQNYPNPFNPTTTIPVRLDRAGEARLD
                     VFDLTGRRVRTLHAGLLPAGPLEFRFDAGSLPSGIYLYRLQTTSGTATRSMALIR"
     gene            901..1758
                     /locus_tag="AC16YU_08140"
     CDS             901..1758
                     /locus_tag="AC16YU_08140"
                     /inference="COORDINATES: protein motif:HMM:NF047050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6920 family protein"
                     /protein_id="HIR6065233.1"
                     /translation="MLALLARIVLALVLVTVGVWGAGILRWRALTLRRRNALQAHARA
                     GGGAAPGGAPDPELPAPVARYLSLALGGEPRLVRTARVRHAGQFRMSDSPTGWRPFQS
                     EQWVTVVPPGMLWNARIRMNPALTTRVHDAYHGGEGLLHATLLGLVTVVRRSGSRPLA
                     EAELMRYLAEAVWYPTALLPVCGVRWEAVDASTARATLSDGSVTASLEFVFGADGLVE
                     SVRSAARYRGERNGEAQFRPWRGRFSSYGEWGGMRIPARGEVAWELDGREVAYWRGRI
                     RSIGFGYGD"
     gene            1862..4003
                     /locus_tag="AC16YU_08145"
     CDS             1862..4003
                     /locus_tag="AC16YU_08145"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6065234.1"
                     /translation="MRRTRFTGTVLGLALLASACTPDSPMPIDSDNPVMAEWTGPYGG
                     VPAFDQVRVEHFVPALETAMASMTADIDAITANPESPTFENTLAALERAGRVLDRAET
                     VFSVWGSSMSTDEFQAVEREMAPRMAAFYDGITQNQALFDRIRAVYESPVHASLSSEQ
                     KRLAWLVYTNFVRSGAALDAEGKERMAAVNQRLAELYTAFSQNVLADENDYTLELATE
                     DELEGLPASLRAAAAAAGEAAGRPGTWIIRNTRSSVEPFLTFSTRRDLRERAWRMWVM
                     RGDMGDEEDNNALITEIMALRAERATLLGYETHAHWRLERAMARTPERAMELMEAMWT
                     PAVAAVRTEVRDMLAIAGGRDGLTALEPWDYRYYAEILRKERYDLDENEVKPYLQLDS
                     LREGMFWVAGELYGLEFEPADDVPVFHPDVRVWEVRSGGRHVGLFYFDPFAREGKSSG
                     AWMNAYRRQSRFDGRRTVIVSNNANFVRGAEGEPVLVSWSDATTLFHEFGHALHGLLS
                     DVTYPSLSGTAVARDFVELPSQLSEHWLSTPEVLGRFATHVETGEPIPDDLVERIRRA
                     STFRQGFDTVEFLASAIVDMEMHLAGDTPIDAKVFEREALAELGMPKEIVMRHRLPHF
                     AHVFAGDGYSAGYYSYLWADVLVADAWEAFLEAGGPWDLEVAGRLRETILSRGNTSDP
                     AEAWNAFRGRDADIAPLMRKRGFAAPLEPSS"
     gene            4058..4822
                     /locus_tag="AC16YU_08150"
     CDS             4058..4822
                     /locus_tag="AC16YU_08150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6065235.1"
                     /translation="MTIDFGGQGVLVTGASRGIGRAVAGAFAEAGARVALNYRSDDAG
                     AARALAGLPGRGHVPVRADVATPEGAEAAVLGAIEALGRLDVVVNNAAVRGHHPPATT
                     AYGDWVADWDRVLACNLTGAANVCFCAARYMIARGGGRIVNISSRGAFRGEPDMPAYG
                     ASKAGLNALSQSLAVALAPHGIFVGVVAPGFVQTEGTAGRLAGPEGDAIRAQSPLGRV
                     ARADEVARAVLLLASDGMEFATGSILDVNGASYLRS"
     gene            4914..6386
                     /locus_tag="AC16YU_08155"
     CDS             4914..6386
                     /locus_tag="AC16YU_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065236.1"
                     /translation="MTRTLALLLTVLVPSTVQAQTCMDGLGCIDFPNSGAPGAQEAFH
                     RGVLLLHSFEFGPAAEAFREARAIDPGFALAYWGEAMTHNHPLWREVNVDAARRVLSE
                     LAPTPGERAARAGTDREAGYIRAVDALYGEGAKTERDRAYMDAMEELHAAHPGDREAE
                     AFYALSILGSRNGERDFATYMRAAAVALRVFQDNPRHPGAAHYIIHSFDDPVHAPLGL
                     PAADAYSGIAPDAAHAQHMTSHIFVAMGLWDRVVEANINASRVQDAERAESGMGPNVC
                     GHYSSWLHYGRLMKGELEEAETLMDACHARVAGGSASGGEWSYFVTMRARHVVDTADW
                     ALSGRWTADPPAWSDAYADEYVGVYGGPAFTYGLTDAVAALRRGDPAPARAMLETDWA
                     PSPGRSLQLDQLRGLLAIADGRTDDGLDLLAAAAEAEDALPFEYGPPEVVVPTFELLG
                     QELERAGRPADARRAYRRAADRTPGRAAAAGGSGLPHPNH"
     gene            6503..>6843
                     /locus_tag="AC16YU_08160"
     CDS             6503..>6843
                     /locus_tag="AC16YU_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065237.1"
                     /translation="MGSVAKGFIGLTGILLGAVIIVFLVPRYLEEPEPNQVVSGTVAE
                     VRMPNRADIVLVLRGDERTYLVDDGITNGRPFGEWGDALVERPVDLHIARFRWSSDRP
                     REQNVPVLAVYL"
BASE COUNT          960 a         2439 c         2433 g         1011 t
ORIGIN      
        1 ttcgccacgc tcacggtcga cgagatcctg gcgccgcgca ccaccgacga tgcgctcgac
       61 ctcgtctccg ggtgggacct gctgacgctc gccgccatcg aggagcggaa gaaactctac
      121 gtcaaccagt tcggctgctc cgagatggac atggtcctga gcgacgacac gggcgatctg
      181 gcggcgtact acgcgaccgg ggagaccgcc gagttcagcg gcggcgtgcg ggtggccgtg
      241 ggcgacgtga acggggacgg tacctgtacg gtggcgctcc tcgtcccggc cgtccagaaa
      301 atccgtgctt cgacccaggg ccacggcgac accgagtggc ggttcgaggc gtacctgcct
      361 ctcgagaacg gccggatgga cgcggccgtc tctccgcccg tcgccctgtc cctcggatcg
      421 aaggcgacct acgacgacga tgcgggcggc ggcatcggca cgagcgtgct cctcgacgcg
      481 gacggcgacg gcgaggtgga catcacgttc cccctcggcc ggacgaccgt cggcacggac
      541 ccgcggcggg aagtgcccga ggaccacgcc ctggaccaga actacccgaa tcccttcaac
      601 ccgacgacca cgatccccgt tcggctcgac cgggccgggg aggcacgcct ggacgtgttc
      661 gacctcaccg ggcgccgcgt ccgcaccctt cacgccgggc tgcttccggc cggaccgctc
      721 gagttccgct tcgatgcggg ctcgctcccg agcggtatct acctgtaccg gctgcagacc
      781 acgtcgggca cggccacgcg gtccatggcg ctgatccgct gattcgaacg gcgggcgcgc
      841 cggagccttg cgggtgagcc gcgtgaccgg ttccttggac cgcacggcct cccgcaatcg
      901 atgctcgctc tgctcgcccg gatcgtcctc gccctcgtcc tcgtgacggt gggcgtctgg
      961 ggggccggca tcctccgctg gcgcgccctg accctccggc ggcggaacgc gctccaggcg
     1021 cacgcccggg ccgggggcgg cgcggcgccg ggaggcgccc cggatccgga gcttcccgcc
     1081 ccggtggcgc ggtatctgtc gctcgccctg ggcggagagc cgcgcctcgt gcgcacggcg
     1141 cgcgtccggc acgccgggca gttccgcatg agcgattcgc cgaccggctg gaggccgttc
     1201 cagtccgagc agtgggtgac ggtggttccg cccggcatgc tctggaacgc ccggatccgg
     1261 atgaatcccg cgctcacgac ccgtgtccac gatgcctacc acggcggaga gggactgctg
     1321 cacgccacgc tgctcggcct cgtgaccgtg gtccggaggt cgggctcgcg accgctcgcc
     1381 gaggccgagc tcatgcgcta cctggccgag gccgtatggt atcccacagc cctcctgccc
     1441 gtgtgcgggg tgcgctggga ggccgtggac gcgtcaaccg cgcgcgccac gctatcggac
     1501 ggatcggtca ccgcctcgct ggagttcgtg ttcggggcgg acggcctcgt cgagtcggtg
     1561 cgctcggcgg ctcgatatcg gggcgagagg aacggtgagg cacagttcag gccgtggcgc
     1621 ggccgcttct cctcgtacgg agagtggggc ggcatgagga ttcccgcccg gggagaggtc
     1681 gcgtgggaac tagacggtcg ggaggtggct tactggcgtg gacggatccg gtccatcggg
     1741 ttcggatacg gcgactgacc ggggattgcc cgacacggcg cgccgccatc cgccggattc
     1801 ccgtggaccg ggccgcggct agcttgacgg aattccgttt cttccgcccg ccgttacgac
     1861 catgcgacgc actcgcttca cgggcaccgt tctcggactc gccctcctcg cttccgcctg
     1921 cacccccgac tcccccatgc cgatcgattc cgacaatccc gtcatggccg agtggacggg
     1981 accgtacggc ggcgtgccgg ccttcgacca ggtccgggtc gaacacttcg tgcccgccct
     2041 cgaaacggcc atggcctcga tgaccgcgga tatcgacgcc atcacggcca accccgagag
     2101 ccccacgttc gagaacaccc tcgccgcgct cgagcgcgcc ggccgcgtgc tggaccgggc
     2161 cgagaccgtg ttctccgtct ggggctcgag catgagcacc gacgagttcc aggcggtcga
     2221 gagggaaatg gcaccgcgga tggccgcctt ctacgacggc atcacgcaga accaggcgct
     2281 gttcgatcgc atccgcgcgg tctacgagtc gccggtgcat gcgtcgctct cgtccgagca
     2341 gaagcgcctg gcctggctcg tctacacgaa tttcgtccgg tcgggcgccg cgctcgatgc
     2401 cgaaggaaag gagcgcatgg cggccgtcaa ccagcgcctc gccgagctct acacggcctt
     2461 ctcgcagaac gtgctggccg acgagaacga ctacacgctc gaactcgcga cggaggacga
     2521 gctcgagggc cttcccgcat cgctgcgagc ggcggccgcg gcggcaggcg aggccgcggg
     2581 caggccgggc acgtggatca tccggaacac ccgatcatcg gtcgagccct tcctcacgtt
     2641 ctcgacccgg cgcgacctgc gcgagcgggc ctggcgcatg tgggtcatgc gcggggacat
     2701 gggcgacgaa gaggacaaca acgcgctcat caccgagatc atggcgctgc gcgccgagcg
     2761 ggcgacgctc ctcggatacg agacccatgc ccactggcgc ctggaacggg cgatggcccg
     2821 gacacccgag cgggccatgg agctgatgga ggccatgtgg acgcccgccg tggccgccgt
     2881 ccggaccgag gtccgggaca tgctggccat cgcgggcggg cgggacggcc tcacggccct
     2941 ggagccctgg gactaccgct actacgccga gatcttgcgc aaggagcgct acgacctgga
     3001 cgagaacgag gtcaagccgt acctgcagct cgactcgctg cgcgagggca tgttctgggt
     3061 ggccggcgag ctgtacgggc tcgagttcga gccggcggac gacgtgcccg tcttccatcc
     3121 cgacgtccgc gtgtgggagg tgcgctcggg cggtcgccac gtcggcctct tctacttcga
     3181 tcccttcgcc cgcgagggca agtcgtccgg cgcctggatg aacgcgtatc gcaggcagtc
     3241 ccggttcgac gggcgccgca ccgtgatcgt gtcgaacaac gcgaacttcg tccggggcgc
     3301 cgagggagag ccggtcctcg tgtcgtggtc ggacgcgacg actctcttcc acgagttcgg
     3361 gcacgcgctg cacgggctgc tttcggacgt gacctacccg tcgctctccg gcacggccgt
     3421 cgcccgggac ttcgtggagc tcccgtcaca gctctccgag cactggctgt cgacccccga
     3481 ggtcctcgga cggttcgcga cgcacgtcga gaccggtgag ccgatcccgg acgacctggt
     3541 cgagcgcatc cggcgggcct cgaccttccg gcagggcttc gacacggtcg agttcctggc
     3601 gagcgcgatc gtcgacatgg agatgcacct cgccggcgac acgccgatcg acgcgaaggt
     3661 gttcgaacgc gaggcgctcg cggagctcgg catgccgaag gaaatcgtga tgcggcatcg
     3721 ccttccccac ttcgcccacg tcttcgcggg tgacgggtac tcggccgggt actacagcta
     3781 cctgtgggcc gacgtcctgg tggccgacgc ctgggaagcg ttcctggaag cgggcgggcc
     3841 ctgggacctc gaggtggcgg gccggctccg tgagaccatc ctctcgcgcg gcaacacctc
     3901 cgacccggcc gaggcctgga acgcgttccg cgggcgggac gcggacattg cgccgctgat
     3961 gcgcaagcgc ggcttcgcgg caccgctgga gccctcgtcg tgacgcgccg ggcggcgcgt
     4021 cgtacgttcc cgccgtgacc cttcgcctgg gagagccatg accatcgact tcgggggaca
     4081 gggcgtcctc gtgacgggcg cctcgcgcgg aattggccgt gccgtggccg gcgcgttcgc
     4141 cgaggcgggc gcacgggtcg ccctgaatta ccgctccgac gacgccgggg ccgcccgcgc
     4201 gctggccggg ctcccgggcc gcggacacgt cccggtccgg gcggacgtcg cgaccccgga
     4261 aggggccgag gccgccgtgc tcggggccat cgaggcgctc ggccgcctgg acgtcgtggt
     4321 gaacaacgcg gcggtgcgcg ggcaccaccc tccggccacg accgcctacg gggactgggt
     4381 cgccgactgg gaccgagttc tggcctgcaa cctgacaggt gccgcgaatg tgtgtttctg
     4441 cgccgcgagg tacatgatcg cccgcggcgg aggccggatc gtgaacatat cctcccgggg
     4501 cgccttccgg ggagagcccg acatgccggc ctacggtgcg tcgaaagccg gcctcaacgc
     4561 cctgagccag tcgctcgccg tcgcgctcgc cccccacggc atcttcgtgg gagtcgtggc
     4621 cccgggattc gtccagacgg agggcacggc cggccggctc gccgggccgg agggggacgc
     4681 catccgggcg cagagtccgc tcggccgcgt cgcgcgcgcc gacgaggtgg cccgggccgt
     4741 cctgctcctg gcgtccgacg gcatggagtt cgcgacgggg tcgatcctgg acgtgaacgg
     4801 cgcgtcctac ctgcgatcgt aggccttgcg ccgggcagga tgaatcgtcg ttattggcgg
     4861 ggccggccct tgcccccgga ccggccccgt gccatcacct atcgcccgga gccatgaccc
     4921 gcacccttgc gctgcttctg accgttctcg ttccgtccac cgtccaggcc cagacgtgca
     4981 tggacgggct cggatgcatc gactttccca actccggcgc gcccggggcg caggaggcct
     5041 tccaccgggg cgtgctgctg ctccactcgt tcgagttcgg gccggcggcc gaggccttcc
     5101 gcgaggcccg cgcgatcgat cccggcttcg ccctcgccta ctggggcgag gcgatgactc
     5161 acaaccaccc gctctggcgc gaggtgaacg tcgacgccgc ccggcgcgtg ctctccgagc
     5221 tggcgccgac ccccggcgag cgcgccgcaa gggcgggcac cgatcgcgaa gcggggtaca
     5281 tccgggcggt cgacgcgctc tacggcgagg gcgccaagac cgagcgcgac cgcgcctaca
     5341 tggacgcgat ggaggagctg cacgccgcgc atccgggcga ccgcgaggcg gaagccttct
     5401 acgccctctc gatcctgggc agccggaacg gcgagcgtga tttcgcgacc tacatgcggg
     5461 ccgccgccgt cgcgctgcgg gtgttccagg acaacccgcg gcacccgggc gccgcgcact
     5521 acatcatcca ctcattcgac gacccggtcc acgcgccgct cggcctgccc gccgccgacg
     5581 cgtactcggg catcgctccc gacgccgccc acgcgcagca catgacctcg cacatcttcg
     5641 tcgcgatggg cctgtgggac cgcgtggtcg aggcgaacat caacgcatcg cgcgtacagg
     5701 acgccgagcg cgccgaaagc ggcatggggc ccaacgtctg cgggcactac tcgtcctggc
     5761 tgcactacgg acgtctcatg aagggtgagc tcgaggaagc cgagacgctc atggatgcat
     5821 gccacgcccg tgtcgcgggc ggctcggcct cgggcggcga gtggtcgtac ttcgtgacga
     5881 tgcgggcgcg ccacgtcgtg gacacggcgg actgggcact ctccgggcgc tggaccgccg
     5941 atccgcccgc ctggtccgac gcatacgcgg acgaatacgt gggcgtgtac ggcggcccgg
     6001 ccttcacgta cgggctgacc gatgcagtgg ctgcgctcag gcgcggcgat cccgcgccgg
     6061 cacgggcgat gctcgagacc gactgggcgc cgagcccggg ccgctcgctg cagctcgacc
     6121 agctccgggg tctcctcgcg atcgcggacg ggcgcaccga cgacgggctc gacctcctgg
     6181 ctgcggccgc cgaggccgag gacgcgcttc cgttcgagta cgggccgccg gaggtggtcg
     6241 tcccgacctt cgagctgctc ggacaggagc tcgagcgcgc ggggcgtccg gcggatgcaa
     6301 ggcgtgcgta ccgccgggcc gcagaccgga ctccggggcg ggccgctgcc gcgggcgggt
     6361 cgggtttgcc ccatccgaac cactgaatcc ggcaatgccc ggggcacttc gagggccccg
     6421 atgcgtccgg gccccggttg gacttgaacg ggagtgcgcc cgatccggta tcttgggtca
     6481 atcaccggga gggggaaacg cgatgggatc ggtagcgaag ggcttcatcg ggctgacggg
     6541 catcctgctg ggtgcggtca tcatcgtgtt cctcgttccg cgttatctcg aggagcccga
     6601 gccgaaccag gtcgtatccg gaaccgtggc cgaggtgcgg atgcccaacc gtgcggacat
     6661 cgtcctcgtc ctgcgcggtg acgagcggac gtacctggtc gacgacggca tcacgaacgg
     6721 ccgccccttc ggcgagtggg gcgacgcgct ggtcgaacgt ccggtggacc tccacatcgc
     6781 ccggttcagg tggtcatccg accgcccgag agagcagaac gtgccggtcc tggccgtgta
     6841 cct
//
LOCUS       DBNHQZ010000304         3026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_769509, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000304 DBNHQZ010000000
VERSION     DBNHQZ010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_769509"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1218
                     /locus_tag="AC16YU_08165"
     CDS             61..1218
                     /locus_tag="AC16YU_08165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6065238.1"
                     /translation="MRSVLTAGVLLGLALPAGARQIQEVGTATTFDVATWNIEWFGST
                     GGGPTDEARQLQNAADVIEQAGIDLWAVQEIASSGAFADLLSLLGPAWEGELATSSGD
                     GSQRIGFLYRTDVVRKRSSGHILVQFSDEFAGRPPLRLEVDVTLPDTALTVVLVTVHM
                     KAFDDLASYEKRVAAAGRLKNHFDISERTANLVWLGDLNDELLSSITAGKPSPYAAFV
                     GDSEDYRFLSMPLEIAGECTFCGGSVASTIDHVLVSDELFRAADAGTTDRLDAVIAGF
                     PGFLSTTSDHVPVYARLLPEASSIPVESVPAAGRLAVYPVPARGYVTVTLGRPASGPD
                     RIRIVDLLGRERAGAVIEPGHRTATIDVSALAPGPYLLVTPEGSRLVPVLR"
     gene            complement(1274..1465)
                     /locus_tag="AC16YU_08170"
     CDS             complement(1274..1465)
                     /locus_tag="AC16YU_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065239.1"
                     /translation="MKRDLGPILQGSALRGGSPQMRQFISNFCRNVLEDYRRAGYPFG
                     TSMDGLLVWLEFGQKTTDS"
     gene            complement(1535..2047)
                     /locus_tag="AC16YU_08175"
     CDS             complement(1535..2047)
                     /locus_tag="AC16YU_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065240.1"
                     /translation="MKHLSAILLIPLLVLGTSGCIVDTDVDPEDLPAGTGSVYTFDLI
                     FSMDDASFNGPVASVAFDVPEIDAWTTAEGAVLVFFRDQGTWTALPYTFGVESPDLPA
                     VDYTIAMGYGYEVGVLEAFYEASTEAVALEDLPDRQLRVVIIEAFPYGKRAVDLTDWE
                     AVKAHFGLRD"
     gene            2314..>3026
                     /locus_tag="AC16YU_08180"
     CDS             2314..>3026
                     /locus_tag="AC16YU_08180"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR6065241.1"
                     /translation="MTYAVIMAGGIGSRFWPRSRRARPKQFLSVFGDATLLQNTVARL
                     EGLIPPERCFVVTNARYVEQTREQLPGLPPENILGEPISRNTAPCIAFAAHRLAAIDP
                     EATMVVLPADHVITDVASFHAVLRAAIDQSAEQGALVTIGIEPTYPATGYGYVQFEDG
                     GSGPPSSPRPVRTFAEKPDVATAERFIDSGDFLWNSGMFVWRVDTILGALERYLPDTF
                     EAFRPLSEALGTTGEADAVV"
BASE COUNT          479 a         1071 c          987 g          489 t
ORIGIN      
        1 gcggataccg gaaacgggcg catctcccgc ttccgcctga acaccgactt cgagtagcgc
       61 atgcgttcag tcctgacggc gggagtgctc ctgggcctgg cgctccccgc cggggcgcgg
      121 cagatccagg aggtcggaac ggctacgacc ttcgatgtcg cgacgtggaa catcgagtgg
      181 ttcgggagca cgggtggcgg cccgacggac gaggccaggc agctccagaa cgccgccgac
      241 gtcatcgaac aggccggcat cgacctgtgg gccgtgcagg aaatcgcgag ctcgggcgcc
      301 ttcgccgacc ttctctccct cctcggcccg gcctgggagg gcgagctcgc cacgagctcg
      361 ggcgacggga gccagcggat cgggttcctg taccggacgg acgtcgtgcg gaagcgcagt
      421 tcggggcaca tcctggtcca gttctccgac gagttcgccg gccgcccgcc gctccggctc
      481 gaggtggacg tcacccttcc cgacacggcg ttgacggtcg tgctcgtgac ggtccacatg
      541 aaggcgttcg acgatctcgc ctcgtacgag aagcgggtcg ccgcggccgg ccggctcaag
      601 aaccacttcg acatcagcga gcgaacagcg aacctcgtgt ggctcggcga cctgaacgac
      661 gagctcctct cctccatcac ggccggcaag ccctcgccct acgcggcgtt cgtgggcgac
      721 tccgaggact accgcttcct gtcgatgccg ctcgaaatcg ccggggagtg cacgttctgc
      781 ggaggctcgg tcgcgtcgac catcgaccac gtcctcgtct cggacgagct gttccgggcg
      841 gccgacgccg ggacgaccga ccggctcgac gccgtgatcg ccggcttccc gggattcctg
      901 tcgaccacct ccgaccacgt gcccgtctat gccaggctgc tccccgaggc ctcgtccatc
      961 ccggtggagt ccgtgcccgc ggccgggcga ctcgcggtct accccgttcc ggcccgcgga
     1021 tatgtcacgg tgacgctcgg ccggccggcc tccgggccgg accggatccg gatcgtggat
     1081 ctcctgggca gggagcgggc gggcgccgtg atcgagcccg gccaccggac cgccacgatc
     1141 gacgtgtcgg ccctcgcccc gggaccctac ctcctcgtga cgcccgaggg cagccggctg
     1201 gttccggtcc ttcggtaaaa aggaacggcc cgaccgcggg agcggccggg ccatctatca
     1261 agaacggaca cgctcagctg tccgtggtct tctgaccgaa ttcgagccac acgagcagcc
     1321 cgtccatcga ggtgccgaac ggataccccg cgcggcgata gtcctcgagc acgttgcggc
     1381 agaaattcga gatgaactgc cgcatctgtg ggcttcctcc gcgcagggcg gagccctgga
     1441 ggatcgggcc gagatcgcgt ttcaaggtgg ttaccttctg aacaggacta cacgcgatat
     1501 cgacccgaac ccccatggct aaagtgccca caggtcagtc gcggaggccg aaatgggcct
     1561 tcacggcctc ccagtcggtc aggtccacgg ccctcttgcc gtacggaaag gcctcgatga
     1621 tgaccacgcg gagctgccgg tccggcaggt cctcgagggc caccgcctcg gtcgacgcct
     1681 cgtagaaggc ctccagcacg ccgacctcgt acccgtatcc catcgcgatc gtgtagtcga
     1741 cggcgggcag gtccggggac tcgacgccga acgtgtacgg gagcgccgtc caggtcccct
     1801 ggtcacggaa gaacacgagc acggctccct cggcggtcgt ccatgcgtcg atctccggca
     1861 cgtcgaacgc caccgacgcc accggcccgt tgaacgaagc gtcgtccatc gagaagatca
     1921 ggtcgaaggt gtagaccgat cccgtcccgg ccgggagatc ttccgggtca acgtccgtat
     1981 cgacgatgca gccggacgtg cccaggacga gcaatgggat gagcaggatc gctgacaggt
     2041 gtttcatgac gtgcctcttg gtcaggttgc ggtggattcg ggtttcgtta cggatcggat
     2101 gccattcaaa ccccgggccg gagcgctcct acgtcgcccg gcgcgaaatg ggtgcctctc
     2161 cggtcacttt ttctctgcat ccgtgtccat catgatggac aggggccgtc ccggagccgc
     2221 gccggccggg cggcggccgg gcggcggacc cggggtcaat cctcacctgt cccgtccgta
     2281 ccttgctcgc cctttccatc agcgcatcgc ttcatgacct atgccgtgat catggccggg
     2341 gggatcggga gccgcttctg gccccgcagt cgccgggccc ggccgaagca gttcctgagc
     2401 gtattcgggg acgccacgct gctccagaac acggtggccc gcctcgaggg cctcattccg
     2461 ccggagcgct gcttcgtggt cacgaacgcc cggtacgtgg agcagacccg cgagcagctg
     2521 cccggcctcc cccccgaaaa catcctcgga gaaccgatca gccgaaacac ggcgccctgc
     2581 atcgccttcg ccgcgcatcg cctggcagcg atcgatcccg aagccaccat ggtcgtgctt
     2641 ccggccgatc acgtgatcac ggacgtcgct tccttccacg cggtgctccg tgccgccatc
     2701 gaccagtcgg ccgaacaggg tgccctggta acgataggca tcgagcccac gtacccggcc
     2761 acgggatacg ggtacgtcca gttcgaggac ggcggatcgg gccccccgtc ctccccgagg
     2821 ccggtccgca ccttcgccga gaagccggac gtcgcaaccg ccgagcggtt cattgattcc
     2881 ggggacttcc tgtggaactc cggcatgttc gtctggcggg tggacacgat cctcggcgcg
     2941 ctcgagcgat acctgcccga caccttcgag gcgttccgcc ctctgtcgga ggccctcgga
     3001 acgacgggcg aagccgacgc cgtcgt
//
LOCUS       DBNHQZ010000305         3508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2935253, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000305 DBNHQZ010000000
VERSION     DBNHQZ010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2935253"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="AC16YU_08185"
     CDS             <1..502
                     /locus_tag="AC16YU_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065242.1"
                     /translation="SGTTSLVGSTREWSTETERDASASFSLRERDGHTELEIFWTESD
                     ILPIPFITTPLVLLMLSIPLLSEGMGLGGAPFAVGMALMATILFFASRGVFRTLTSGH
                     RRGVRQFADQLVDIAHQERGRAPSAARQSATAVDAPAPRIALDEPPDAESEDRAPTDR
                     PRTRTP"
     gene            complement(507..1925)
                     /locus_tag="AC16YU_08190"
     CDS             complement(507..1925)
                     /locus_tag="AC16YU_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR6065243.1"
                     /translation="MRLHLLILFLVLASCGGGGRTASDGRTVRFEIAMTGPSAEGWFD
                     GAADSVGIRGSAPPLSWDESYPMTDEDGDDVWEAEVTFAEDAPTIIQYKLFVEGEGNP
                     NGGWERGDNRWLNRETVRTVRRPYEDSPEPLPATYTGTIVEHTGFGSGDSVRARDLIV
                     WLPPGYEADAGHRYPVLYLHDGQNMFDRRAVGAEWGMDETATRLIEEGAIEPVILVGV
                     ANTADRMLDYTPVPGGFAMRFERTGEGRGDSPLAGTFRMVGDEAIQVEIRGNARLEVR
                     WAGESDWVPGFRTDDRWYVPSMETYVTADETADGRVQRFTAKRDMGGGGGRAYARFLV
                     EEVKPFIDATYRTLPDRASTSVGGSSLGGLITMSIGTWHPDVFGGLLVVSPSVWWRDT
                     AILDMVREAPFAERPRIWLDMGTAEGPLALQGARELRDLLIGKGWTEGEDLAYLEDVG
                     APHNEDAWAGRAEGMLRFLYGR"
     gene            complement(2110..>3508)
                     /locus_tag="AC16YU_08195"
     CDS             complement(2110..>3508)
                     /locus_tag="AC16YU_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6065244.1"
                     /translation="AVCAVMLLAPAPAGLGDPGWRVAAVAVLMAVWWITEAIPIPVTS
                     LVPVAMLPLIGAGSIREATTPYADPVIYLFLGGFMIALAMERWSLHRRIALHVIRRVG
                     TSPRALVAGFMLAAALLSMWVSNTATALMMLPVGISVVELAERTGGPGTRERFAVCLL
                     LGIAYGCNIGGMGTLVGTPPNVLMAGFFEQEYGRSIGFAEWLKVGLPVVAVGLPLAFL
                     VLTRVTNPIRIRELPGARETVETELAELGPFSRGEAVVAVVFGLTATLWITRPLLEDL
                     IPGLSDAGIAIAAALALFVIPVRLSEGEFAMDWRTARRLPWGTLLLFGGGLSLAGAFT
                     RTGLSLWVGESLAGVGSLPLVVVVLVVTAVVVFLTELTSNTATAATFLPVIAALALGI
                     GQDPMVLALPAVLGASCAFMLPVATPPNAIVYGSGRVSIPQMARAGFVLNLVFIAVIT
                     ALAYSVFGWVMGGAG"
BASE COUNT          571 a         1298 c         1162 g          477 t
ORIGIN      
        1 gtcgggcacg acgagcctgg tgggctccac gcgggaatgg tcgacggaga ccgagcgcga
       61 cgcttccgcg agcttcagcc tgcgcgaacg cgacgggcac acggagctgg agatcttctg
      121 gaccgagagc gacatcctgc ccatcccgtt catcacgact ccccttgttc tgctcatgct
      181 gtccatcccg ctcctctcgg agggcatggg cctcggcgga gcgccgttcg ccgtcgggat
      241 ggccctgatg gccaccatcc tgttcttcgc atcccggggt gtattccgca ctctcacgtc
      301 gggccatcgc cgcggggtcc gccagttcgc ggaccagctg gtggacatcg cccaccagga
      361 gcgaggacgg gcgccctccg cggcacgaca gtccgcgacg gccgtcgatg cgccggcacc
      421 gcggatcgcg ctcgacgagc cgcccgacgc cgagtccgaa gatcgcgctc cgacggatcg
      481 cccgcggaca cgcacgccct gacgcgttat ctcccgtaca ggaagcgcag catgccctcg
      541 gcccgtccgg cccaggcgtc ctcgttgtgt ggcgccccga cgtcctcgag ataggccagg
      601 tcctcgccct ccgtccagcc cttcccgatc agcaggtcgc gcagttcacg cgctccctgc
      661 agggccagcg ggccctcggc cgtgcccatg tcgagccaga tgcggggccg ctcggcgaac
      721 ggtgcctcgc gcaccatgtc caggatcgcc gtgtcccgcc accagaccga cggcgagacc
      781 accagcagcc cgccgaacac gtccggatgc caggtgccga tcgacatcgt gatcaggccc
      841 ccgagcgacg atccgccgac cgaagtgctc gcgcggtccg gcagcgtgcg gtacgtcgcg
      901 tcgatgaacg gcttgacctc ctcgaccagg aatcgggcgt atgcccttcc tccaccgccg
      961 cccatgtccc gcttcgcggt gaagcgctgc acgcggccgt cggcggtttc atcggccgtg
     1021 acgtacgtct ccatcgacgg aacgtaccac cggtcgtccg tccggaagcc gggaacccag
     1081 tcgctctctc ccgcccagcg cacctccagc cgggcattgc cccggatctc gacctggatc
     1141 gcctcgtcgc cgaccatccg gaacgtcccg gcaagcgggc tgtcgccacg cccctcgccc
     1201 gtgcgctcga accgcatggc gaagcctccg ggaacgggcg tgtagtcgag catccggtcg
     1261 gccgtgttcg cgacgcccac caggatgacc ggctcgatcg ccccctcctc gatgagccgg
     1321 gtggccgtct cgtccatgcc ccactccgcg cccacggccc ggcggtcgaa catgttctgc
     1381 ccgtcatgca ggtagagcac ggggtagcgg tgcccggcgt ccgcctcgta tcccggggga
     1441 agccacacga tgaggtcgcg cgccctgacc gaatcgccgc tcccgaaccc cgtgtgttcc
     1501 acgatcgtgc ccgtatacgt cgcgggaagg ggctcggggg agtcctcgta cgggcgccgt
     1561 accgtgcgca cggtctcccg gttcagccat cggttgtcgc cgcgttccca tccgccgttc
     1621 ggattgcctt cgccctccac gaaaagcttg tactggatga tggtcggggc gtcctcggcg
     1681 aaggtgactt ccgcctccca gacgtcgtcc ccgtcctcgt ccgtcatcgg gtaggattcg
     1741 tcccacgaga gcggcggggc cgacccgcgg atgccgaccg agtccgcggc gccgtcgaac
     1801 cagccctccg ccgatggacc cgtcatcgcg atctcgaaac gaacggtgcg gccgtcggac
     1861 gcggtccttc cgccgccgcc gcacgaggcc aggaccagaa agaggatcag gagatgcaat
     1921 cgcatggcgg gtgtcgccgc tcgcgggcgg acagggattc tagcggtgaa ggtatcgacg
     1981 gcgccgcgga atcgcagggg gcagtaccgc ctcggggcgt cgcggcgagg cgtaggccct
     2041 ccgtatcggc gtatccgcgc acgaggcgcg aggttgcagg tgtctctgcc acaacgcgta
     2101 tgccgggcgt caccccgcgc cgcccatgac ccatccaaag accgagtacg ccagcgcggt
     2161 gatgaccgcg atgaaaacga ggttcagcac gaaacccgcg cgggccatct gcgggatgct
     2221 gacgcgcccc gacccgtata cgatggcatt cggcggcgtc gcgaccggca gcatgaaggc
     2281 gcagctcgcg cccagcaccg cgggaagcgc cagaaccatg gggtcttgcc cgatgcccag
     2341 cgccagcgcc gcgatgaccg gcaggaacgt ggccgccgta gcggtattcg aggtcagctc
     2401 ggtcaggaag acgacgacag ccgtcaccac caggaccacg accacgagcg gcagcgaacc
     2461 gacgcccgca agcgactccc cgacccagag cgaaagcccg gtccgcgtga acgcgccggc
     2521 gaggctcagc ccgccgccga agagcagcag ggtgccccac ggcaggcggc gcgccgtccg
     2581 ccagtccatc gcgaattcgc cctcggacag gcgcaccggg atcacgaaga gcgcgagcgc
     2641 cgcggctatc gcgatgccgg cgtccgagag tccgggaatc aagtcctcga ggagcggccg
     2701 ggtgatccac agcgtcgccg tcagcccgaa aacgaccgcc acgaccgctt caccccggct
     2761 gaacggcccg agctccgcga gctccgtctc gaccgtttct cgggccccgg gcagctcgcg
     2821 gatgcggatc gggttcgtga cgcgcgtcag caccaggaac gcgagcggca ggcccacggc
     2881 cacgaccggg aggcccacct tgagccactc ggcgaatcct atggagcgcc cgtactcctg
     2941 ctcgaagaac cccgccataa ggacgttggg cggcgtcccg acgagggttc ccatcccccc
     3001 gatgttgcac ccgtaggcga ttcccagcag caggcacacg gcgaagcgct cgcgcgtgcc
     3061 ggggccgccg gtccgctcgg cgagctccac gaccgagatg ccgaccggga gcatcatgag
     3121 cgccgtcgcc gtgttgctca cccacatcga gaggagcgcg gcggcgagca tgaatccggc
     3181 gaccagggcc ctcgggctgg tgccgacccg ccggatcacg tgcagcgcga tgcggcggtg
     3241 cagcgaccag cgctccatcg cgagcgcgat catgaatccg cccaggaaca ggtaaatgac
     3301 gggatccgcg tacggcgtcg tcgcctcgcg gatggacccg gcgccgatga gcggcagcat
     3361 tgcgacggga acgagcgacg tcacgggaat cgggatcgcc tccgtgatcc accacacggc
     3421 catgaggacg gccacggcgg ccacgcgcca tcccggatcg ccgagaccgg ccggggccgg
     3481 cgcgaggagc atcacggcac agaccgca
//
LOCUS       DBNHQZ010000306         5718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2395144, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000306 DBNHQZ010000000
VERSION     DBNHQZ010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2395144"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="AC16YU_08200"
     CDS             <1..446
                     /locus_tag="AC16YU_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6065245.1"
                     /translation="PRLAEMNVRRNVVEIAALMWNHGPQMRAYMQRKGIEWPRFEGGE
                     MADVIAYLYWLGFQDPTGSAAAGRRVFEEKGCATCHESGDANLGPVLPAIAHLQRPER
                     LAQLMWNHAGEMEDLMLARNRPWPQLTEQDMRDLLAFLKSERSRE"
     gene            480..1703
                     /locus_tag="AC16YU_08205"
     CDS             480..1703
                     /locus_tag="AC16YU_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6065246.1"
                     /translation="MKMTRTTPHPATGSRMLTAFVVVLIPVLCLAVMSAGRAEQPVPD
                     DLGRAVFISKGCAGCHAVYGYGIGGSNPSGGPDLGKRMVFGTRLELAAQLWNHLPDML
                     RQMDRRGVEPPQFTTEEVDQVIQYLSFIRYMDQPGNERTGRRLLNGKKCTRCHGMGSE
                     ESKEAPNLAQSGTYTSPLDLATALWNHGPEMEEMFAERDIDRPSFSRLDIVDLAAGIR
                     SFIRTNTVPPEAYEPGDPEVGRVLALEKGCLDCHAVRGMGGDLAPDFIEVNLRKTVTE
                     IAGDMWNHGPKMWELIRDRGMTFPTLERAEMASLIAYLYSLGLDDEPGDAESGASRVN
                     DKRCTACHSVGGAGGDLAGDLTSAGGFESPTAMIAAMWNHASDMADHGRELRIRWPRL
                     SGDDIADIYAFVRSH"
     gene            2232..2642
                     /locus_tag="AC16YU_08210"
     CDS             2232..2642
                     /locus_tag="AC16YU_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065247.1"
                     /translation="MNGVPRHSLLLRVTLSVLLAAWSGYGAVVRGDVFEHQAAPRIDR
                     GAMPDALVPPGGRVPPDGDTASETGLGEAALTYALFVVDASTHEVPVGPAGHGVHAPP
                     VMAGRAGPDALASRQRPGTETRRTRRHLLCVYRL"
     gene            2727..2879
                     /locus_tag="AC16YU_08215"
     CDS             2727..2879
                     /locus_tag="AC16YU_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065248.1"
                     /translation="MATVKQPSAVRRPEASEPTGPNDRVTRVAFNVFMALLVLSLIAM
                     IWIALV"
     gene            3057..3833
                     /locus_tag="AC16YU_08220"
     CDS             3057..3833
                     /locus_tag="AC16YU_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065249.1"
                     /translation="MKRFLAIATLLAGVTSACDTAITEPAGGDGLSLRAASGTAIDIQ
                     AGPAPVVAGKAGKALVCHVGSETGPAGEVYDPACVPGDANGWFCPDAGKVDLIETAAA
                     SRHLGNTAHAYGGISDYAPGAVGASGVGTDDADGDGIDDGCEIPAAAECPCWDEADLE
                     LVTAENLSPDACSGYVGTGGSARAIFSEGEFFQVGFVADPFGEGTGPWCGVLYPVATN
                     QPVSAAEAEACVAQIEARCAEIEGDGPIVVGGAPTARTRR"
     gene            complement(3811..4680)
                     /locus_tag="AC16YU_08225"
     CDS             complement(3811..4680)
                     /locus_tag="AC16YU_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HIR6065250.1"
                     /translation="MYKILTVAAVALTIAGPAHAQTADLVLGARPQGLGGAFTAVADD
                     ANAVYWNPAGLGRLEHREVTFMHWMFADISQVTVDHAAFAQPLGSGALGLSWTRQGAE
                     LEQGLDRTKSTMAVNSFRVAFGMPLGEQVALGVTFNRTLVTSNAPTNGEVAFDGGLLV
                     RPLVDEPWTVGVTARNVGGDQVLLPYYALGTAYRFQWPTRHLLLSVDVHSQEDIQGTE
                     DLVVKYGGGLEFGQTFDDFGLALRGGINSRTFAAGLGLGWRMLAVDYAFVIMREDTIG
                     DSHKVGVTYVFGR"
     gene            complement(4736..>5718)
                     /locus_tag="AC16YU_08230"
     CDS             complement(4736..>5718)
                     /locus_tag="AC16YU_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065251.1"
                     /translation="IYVPEVIVAGSYADVPDAESVHSVRIQVRNAEGHHVNDSPFVLQ
                     PTPDRRFSRVLRLVVGVNDVEVSVRDRAGNETTVVFTLVRIPGSETELVGETGGTVTS
                     PDGTSVVVPEGALLGPQEITIRRFPVSELPVPLSDEVTLVGAGHEFGPSGLVFHEPIT
                     IRLAYADADLDVDQDGAADFDESLLDVFYWDGFRWIKTDASGRSAEENFVSFTTNHFS
                     TYALGVDAQVDEFRMYWSRNPFVASEGTTAVLELPNSGRISLKIYDMEGDLVRTIADD
                     EAVAGSASRNWDGKGDFDGYVGSGIYIYVFEYDDDSGPTRVIRKPIGVVQ"
BASE COUNT          940 a         1950 c         1925 g          903 t
ORIGIN      
        1 gtcccaggct cgccgagatg aacgtgcgcc gaaacgtcgt tgagatcgct gcactcatgt
       61 ggaaccacgg cccgcagatg cgggcgtaca tgcagcggaa ggggatcgag tggccgcgat
      121 tcgagggtgg cgagatggcc gacgtgatag cgtacctgta ctggctcggg tttcaggatc
      181 cgacgggaag tgcagccgcg gggcgccggg tattcgagga aaaggggtgc gccacatgtc
      241 acgagtccgg cgacgcaaac ctcggcccgg tcctcccggc catagcgcat ctgcagcgtc
      301 ccgaacgact ggcccagctc atgtggaacc atgcgggcga gatggaagac ctgatgctgg
      361 caagaaaccg accatggcca caattgacgg aacaggacat gcgtgacctc cttgctttcc
      421 tcaagagcga gcggagtcgg gaatgaccgg gaggcaacgc cagacaaagg taaagcacca
      481 tgaagatgac acgtacgacg ccccacccgg cgaccggctc caggatgttg accgcgttcg
      541 tcgttgttct catcccggtc ctttgtttgg ccgtgatgtc cgcgggccgg gcggagcaac
      601 ccgtgccgga cgacctggga cgagccgtgt tcatatccaa gggttgtgcc ggatgccatg
      661 ccgtgtacgg atacgggatc ggcggcagca acccgtccgg ggggcctgac ctcggaaaac
      721 gcatggtctt cggcacgcgc ctggaactgg ccgcccagct ctggaaccac ctgcccgaca
      781 tgctgagaca gatggatcgt cgaggcgtcg agccacccca gttcacaacc gaggaagtcg
      841 accaggtcat ccagtacctg tcgttcatcc gatacatgga tcagcctgga aacgagcgga
      901 cggggagacg gcttctcaac ggaaagaagt gcacgcgctg ccacggcatg ggtagcgagg
      961 agagcaagga ggcgccgaac ctcgcgcagt ccggtaccta cacttctccg ctggatctcg
     1021 cgacggctct gtggaaccat ggtcctgaaa tggaggagat gttcgcggaa agggacatcg
     1081 atcggccgag cttcagccgc ctcgacatcg tcgatctcgc ggcaggcatc cgctccttca
     1141 taagaaccaa caccgttccg cccgaggcct acgaaccagg ggatcccgag gtgggcaggg
     1201 tgctggcact ggagaagggg tgcctcgact gccacgctgt acggggtatg ggcggtgatc
     1261 tcgcaccgga tttcatcgaa gtgaacctcc ggaagaccgt gacggagatc gcgggagaca
     1321 tgtggaatca cggaccgaag atgtgggaac tcatccgtga ccggggaatg accttcccca
     1381 cactggagcg cgccgaaatg gcatcactga ttgcctacct gtactctctc ggtctggacg
     1441 acgagccggg agacgccgag tcaggggcct cccgcgtgaa cgacaagcgg tgtaccgcct
     1501 gccattcggt cggaggcgct ggtggcgacc tggcagggga tctgacatcg gctggaggct
     1561 tcgagtctcc caccgccatg atcgcagcca tgtggaatca cgcttcggat atggccgacc
     1621 acggacgcga gctgcgaatc cgctggccca ggctgtccgg agacgacatc gccgatatct
     1681 acgcgttcgt tcggagccac tgaccgcgac ccgcccccgg tgactcgccc ggcgagaagt
     1741 ggacaatctg gtgtctgtgg gcccggcgac ctggctgcag cagcgcgaac cggttcagac
     1801 tgtcacatgg atgaagcagc cgctccgagg ccgggccctg gacgagcctg caggtcgggg
     1861 aggccgatcc ggctctaacg gctctcgacc gctgtgagac gacggaccgc ccgccggacg
     1921 gcgcaacccg ggcaagctcg gccgtcgcat ccagccgtgg gtcggtcgta gcgactccca
     1981 cgaaccacga cgtgtccgag atggccccgg gtggaatcat gagcgcggtg caggtggccc
     2041 agagtatagc cacggctccg atcccgggct gccccggtct tcgcctggat cggtcccggc
     2101 ggaaccggat tcgaccctct gcagcggaca gtctccgatc caggaagcgc tccaaccgct
     2161 gcagcgaccg ccgaggaacc gccccgatcc gccaaggttg agcaccccaa tcggccggcg
     2221 catcgccctc gatgaacgga gtcccccgtc atagcctcct gcttcgggtc accctgtcgg
     2281 tgctcctcgc cgcgtggagc gggtacgggg ccgtggtccg gggggacgtg ttcgagcatc
     2341 aggcggcgcc ccggatcgac cgcggtgcca tgcccgacgc cctcgtcccg ccgggcggcc
     2401 gtgtgccgcc ggatggagac accgcgtcgg agacggggct tggcgaggcg gcattgacgt
     2461 acgcgctctt cgtcgtcgat gcttccaccc acgaagtgcc ggtcggaccc gccggccacg
     2521 gtgtccacgc tccgcccgtc atggcgggaa gagcggggcc cgatgccctg gcaagccggc
     2581 agcggcccgg tacggagacc aggcggacgc gccgtcatct cctgtgcgtc tatcggttgt
     2641 agtcgcggcg acggccccgg agcgagggcg atgtccctgg cccggggcga ttcgtttccg
     2701 ccttctcacc gatcgacgca ctacccatgg ctaccgtcaa gcagccctcc gccgttcgcc
     2761 ggccggaggc ctccgaaccc actggaccga acgaccgcgt tacgcgcgtg gcattcaacg
     2821 tcttcatggc cctcctcgtc ctctcgctca tcgcgatgat ctggatcgcc ctcgtctgaa
     2881 agcgagtccg cccgacggca gactcgtggc cattgcgggg cccgggcagg aggtccgggc
     2941 cccgccctat tgatacccgg ccccgtacat cctactctga ccgggtccgg ccccggttcc
     3001 gcatgccccg cgggctcgac cggcggactc ccccctcacc catcggtcag cacgtcatga
     3061 aacgattcct tgcgatcgcc acgctccttg ccggcgtgac ctcggcctgc gacacggcca
     3121 ttaccgagcc cgcaggcggc gacggcctct cgctccgcgc cgcctccggc acggcgatcg
     3181 acatccaggc ggggccggct cccgtcgtcg cggggaaggc cggcaaggcg ctcgtctgcc
     3241 acgtgggcag cgagactggc cccgccggtg aggtctacga cccggcgtgc gtgcccggtg
     3301 acgccaacgg gtggttctgc cctgacgccg gcaaggtcga cctgatcgag accgccgcag
     3361 cctccaggca cctcggcaat accgcgcatg cctacggggg catctcggac tacgcgcccg
     3421 gagcggtcgg agcgagcggc gtcggcacgg acgacgccga cggcgacggc atcgacgacg
     3481 gctgcgagat tccggccgcc gccgaatgcc cctgctggga cgaggccgac ctcgagctgg
     3541 tgaccgcgga gaacctctcg ccggacgcct gctcgggcta cgtgggcacg ggtggctcgg
     3601 cgcgggccat cttcagcgag ggcgagttct tccaggtcgg attcgtcgcc gatccgttcg
     3661 gcgagggtac cgggccgtgg tgcggcgtgc tctatcccgt cgcgacgaac cagcccgtct
     3721 ccgccgccga ggccgaggct tgcgtcgccc agatcgaggc gcgctgcgcg gagatcgagg
     3781 gggatggtcc gatcgtggtc ggcggcgccc ctaccgcccg aacacgtagg tgacgccgac
     3841 cttgtgcgag tcgccgatgg tgtcctcgcg catgatcacg aaggcgtagt ccaccgcgag
     3901 catcctccag ccgagcccca ggcccgcggc gaaggttctc gaattgatgc cgccgcgcag
     3961 cgcgaggccg aagtcgtcga acgtctgtcc gaattccagg ccgccgccgt acttgaccac
     4021 gaggtcctcg gtgccctgga tgtcctcctg cgagtgcacg tccaccgaca ggagcaggtg
     4081 ccgggtcggc cactggaacc ggtacgccgt cccgagcgcg tagtagggca ggagcacctg
     4141 gtcacccccc acgttcctgg ccgtcacccc gacggtccac ggctcgtcga cgagcggccg
     4201 gacgagcagg ccgccgtcga aggccacctc tccgttggtc ggcgcgttcg aggtcacgag
     4261 ggtccggttg aacgtgacgc cgagtgccac ctgctcgccg agcggcatcc cgaaggccac
     4321 ccggaagctg ttcacggcca tggtgctctt cgtccggtcc aggccctgct cgagctccgc
     4381 gccctggcgg gtccacgaga ggccgagggc tcccgagccc aggggctgcg cgaaggcggc
     4441 gtggtccacg gtcacctggc tgatgtccgc gaacatccag tgcatgaagg tcacctcccg
     4501 gtgttccagc cggcccaggc cggccgggtt ccagtagacc gcgttcgcgt cgtcggcgac
     4561 agccgtgaag gccccgccca gtccctgcgg ccgggccccc aggaccaggt cggccgtctg
     4621 cgcgtgcgcg ggcccggcga tcgtcagggc gacggccgcg actgtcagaa tcttgtacat
     4681 cgctttcatt gcgctgtggt ctttggtctt gacagggatt gcgagtccgc gtgaatcact
     4741 ggacgacgcc gatcggcttg cggatcaccc gcgtcgggcc ggaatcgtcg tcgtactcga
     4801 acacgtagat gtagatgccg ctgccgacgt agccgtcgaa atcacccttc ccgtcccagt
     4861 tccggctcgc gcttccggcg acggcctcgt cgtcggcgat cgtccgcacc aggtccccct
     4921 ccatgtcgta gatcttcagg ctgatgcgcc cgctgttcgg cagctccagg accgccgtcg
     4981 tgccctcgga cgcgacgaac gggttgcggg accagtacat ccggaactcg tccacctgcg
     5041 cgtccacgcc cagggcgtag gtcgagaagt ggttggtcgt gaagctcacg aagttctcct
     5101 cggccgagcg gcctgaagcg tccgtcttga tccaccggaa gccgtcccag tagaagacgt
     5161 cgagcaggct ctcgtcgaag tcggccgcgc cgtcctggtc cacgtccagg tcggcgtcgg
     5221 cgtaggcgag ccggatggtg atcggctcgt ggaagaccag gccggacggc ccgaactcgt
     5281 ggcccgcgcc gacgagcgtc acttcgtcgc tgagcgggac gggcagctcc gacacgggga
     5341 atctccggat cgtgatttcc tgcgggccga ggagcgcgcc ttccgggacc acgaccgacg
     5401 tgccgtcggg tgatgtcacg gttccgccgg tctcacccac gagttcggtc tcgctcccgg
     5461 ggatgcgcac caacgtgaat acgaccgtcg tctcgttgcc ggcgcggtcc cggacggaaa
     5521 cctcgacgtc gttcacgccg acgaccaggc gcagcacgcg cgagaaccgc cggtcaggcg
     5581 tgggctggag cacgaacggc gagtcgttca cgtggtgccc ctccgcgttg cgcacctgga
     5641 tgcggaccga gtgcaccgac tccgcgtcgg ggacgtcggc gtacgagccg gcgacgatca
     5701 cctcgggcac gtagatct
//
LOCUS       DBNHQZ010000307         1634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3344510, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000307 DBNHQZ010000000
VERSION     DBNHQZ010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3344510"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC16YU_08235"
     CDS             <1..255
                     /locus_tag="AC16YU_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6065252.1"
                     /translation="CSSDLVTISVPPALVPVVGVLSEWAGRLVGSYPPLNREKAEEIR
                     HATIMCSSARAAADFGYSERIPLAEGIAGTLAWYRSRGWL"
     gene            344..>1634
                     /locus_tag="AC16YU_08240"
     CDS             344..>1634
                     /locus_tag="AC16YU_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR6065253.1"
                     /translation="MSKLGFNTGYIEDLYRRFLADPESVGEAWREFFADFRPDAAFLA
                     AAEARPVATAVEEAPARAPEGDGAAPSAPTPLPEGSEVKPIRGPAAKIVENMEASLGI
                     PTATSVRTVPVKLMAENRRLINDYQKDVGGAKVSFTHMVSWAVVRAIEAFPGMNTTYR
                     NEEGTPGHVFPPAVNLGLAIDIEKRGRRSLVVPNIKGADRLRFSELLGVYNDIVQRAR
                     DSQLELADFEGTTATITNPGMIGTSLSVPRLMPGQGLIVGVGAIGYPTEYFAYPPDLI
                     ARTGISQVMTITSTYDHRVIQGAESGAFLQYIEELFLGKHEFYEGIFSDLGVPYQPFK
                     LAPDTTPQVGRDEAGELGMIRKQARVLQLIRSYRVRGHLHADVNPLGYEWTYHAELDP
                     TQYGLTIWDLDREFVTGGLGAKDILPLRDILDIVRQTY"
BASE COUNT          266 a          584 c          534 g          250 t
ORIGIN      
        1 tgctcttccg atcttgtcac gatttccgtg ccgcccgcac tggtccccgt ggtcggcgtg
       61 ctctccgagt gggccgggcg cctggtcggc tcgtatccgc ccctgaaccg ggagaaggcc
      121 gaggaaatcc ggcacgccac catcatgtgc tccagcgcca gggcggccgc cgacttcggg
      181 tactccgaac ggattccgct ggccgagggc atcgcaggaa ccctcgcctg gtaccggtca
      241 cggggctggc tgtaggcggg cgcggggaat ccttccaccg gccctttcgt tagcacgaat
      301 cccgtttccg cagccaaatc gtcccccgac gggacccctg gacttgagca agctcggatt
      361 caacaccggt tacatcgaag acctgtatcg tcgcttcctg gccgacccgg aaagcgtcgg
      421 cgaggcctgg cgcgagttct tcgccgactt ccgccccgac gccgctttcc tcgcggccgc
      481 cgaggccagg ccggtcgcca cggccgtgga ggaggccccc gcgcgcgccc ccgagggcga
      541 cggcgccgcc ccctccgccc cgacgccgct cccggaggga tccgaggtga agccgatccg
      601 gggcccggcc gcgaagatcg tcgagaacat ggaggccagc ctgggcatcc ccacggccac
      661 ctccgtgcgg acggtcccgg tgaagctcat ggccgagaac cgccggctga tcaacgacta
      721 ccagaaggac gtcggcggcg ccaaggtgtc gttcacgcac atggtctcgt gggcggtcgt
      781 ccgggcgatc gaggcgttcc cgggcatgaa caccacgtac cggaacgagg agggcacgcc
      841 cggccacgtt tttcctccgg ccgtgaacct gggcctggcg atcgacatcg aaaagcgcgg
      901 caggcgatcg ctggtcgtgc cgaacatcaa gggcgccgac cggctccgct tctccgagct
      961 cctgggcgtc tacaacgaca tcgtccagcg ggcgcgggac agccagctcg agctcgccga
     1021 cttcgaaggc accaccgcga ccatcaccaa tcccggcatg atcggcacga gcctgtccgt
     1081 gccgcgcctc atgccggggc agggcctgat cgtgggcgtc ggggccatcg ggtacccgac
     1141 cgagtacttc gcttacccgc ccgacctcat cgcgcggacc ggcatctcgc aggtgatgac
     1201 gatcacgtcc acctacgatc accgggtgat ccagggggcc gagagcggtg ccttcctgca
     1261 gtacatcgag gagctgttcc tcgggaagca cgagttctac gaggggatct tctccgacct
     1321 cggcgtcccc taccagccgt tcaagcttgc gcccgacacg acgccgcagg tgggccggga
     1381 cgaagcgggc gagctgggca tgatccgcaa gcaggcgcgg gtcctgcagc tcatccggtc
     1441 gtaccgggtt cgcggacacc tgcacgccga cgtgaacccg ctcggttacg agtggacgta
     1501 ccacgccgag ctcgatccga cgcagtacgg cctgaccatc tgggacctgg accgcgagtt
     1561 cgtgacgggc ggcctgggcg cgaaggacat cctgccgctt cgcgacatac tggacatcgt
     1621 ccggcagacc taca
//
LOCUS       DBNHQZ010000308        10417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_905773, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000308 DBNHQZ010000000
VERSION     DBNHQZ010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_905773"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16YU_08245"
     CDS             <1..370
                     /locus_tag="AC16YU_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065254.1"
                     /translation="EAARVLAGLAVVGQGPDRTVLFALFAGSRQDDSGARAWLANPTW
                     PADATREVVLVGAPDPGPFPGRAYPVRTIGSDAVAPGSGSLVDRASAQAAALSREVLL
                     AASGMIWHRDGASRVPDDGP"
     gene            392..1141
                     /locus_tag="AC16YU_08250"
     CDS             392..1141
                     /locus_tag="AC16YU_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6065255.1"
                     /translation="MIPSWLPDTVTSAPDRMVERALLWGLEGLELRTVGGPDERVPYV
                     NEARIRRLLTESELAATAVVPGLFEADVDDRASWLNDLALLDETLAFCRRIGSRIVVV
                     SSFRRGAGEDRVAEALRRAGDQAAGRNMALAVLNEPGMGCETGAELGAVLGAAAHPAV
                     RAAWDPACASAAGEEPAEGVRLVARHVALVRHAVGGSSAVDWDAQFAALTERAFDGPV
                     SLVLPPGAVRAEGLAAATGMIGRMRAVDVRR"
     gene            complement(1157..1807)
                     /locus_tag="AC16YU_08255"
     CDS             complement(1157..1807)
                     /locus_tag="AC16YU_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR6065256.1"
                     /translation="MNRIPPLRVRAVLAGTAFALAVVAARPAAGQGDARLTLGVTAFT
                     LSESGPDPEHFDPIARLSAGVGVSFPFRSGFSVRPELHYLTRGAAFDGVVADQEVDVV
                     LELTTLEFGIMAGLDVPLGGTVQPRVFAGPVFGWTAESTLRYRPLAGGSWFSDSYSGV
                     RRTDVAAGFGVEVTIPAWGTRLIAGVRGVVGLTGILEEEPPGSPVPEWRHRGAMST"
     gene            1938..2726
                     /locus_tag="AC16YU_08260"
     CDS             1938..2726
                     /locus_tag="AC16YU_08260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6065257.1"
                     /translation="MNPHPTDESGLQVPITPWTRLHFAWSVAWVAILTAVFAVGYLVT
                     RLARPTAAHFAWWSRHWGRSMMRMMGIRVETSGLHHIEPGTPYVFVANHQNGLDIPVV
                     ADCLPVPFGFVAKAELERVPFLGAALRYSPSVFVDRTDGRRTLESMRSAGEEIRSGSS
                     VLVFPEGARSYDGRMGPFQKGAFLLAVEAGVPIVPLTIVDGPGVFNEKRRVSRPGVIH
                     VVVGQAIALEGVSRRDIPSLMERTARAIAEPLPEDWRNPRTGLP"
     gene            2809..5328
                     /locus_tag="AC16YU_08265"
     CDS             2809..5328
                     /locus_tag="AC16YU_08265"
                     /inference="COORDINATES: protein motif:HMM:NF021126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR6065258.1"
                     /translation="MVARKSPRRAATDAPEIKPSWFEAWDRISPGRRHGVCAAALLVI
                     GVTFMAPVLFGDGRLVGGDIVHWSGMSRALLETEEATGHRPLWAVRPFAGMPAYMISY
                     DLVVPQIDLVAGLLRRLMWPLSHLLILFAGTYLLTFLLTRDRLAGVLAAVAFGLTTYL
                     PVLLIAGHNSKFIALSFLPWLLLAFVHALRRPGLVSGLLFAVAVGLNLRAGHVQITYY
                     GAFIIGVWWLVEAVSALRQGRLRELGRSTLWLAVGGLLGVMMVADPYLVQAEYREFTI
                     RGASSGGGEGGMAWDYAMAWSQGVGELLTLVIADAFGGGGATYWGPKTFTAGPHYSGG
                     IVIMLAAIALARSRQRTVAALAVAGVLMIGFALGENLAVLNRLMFEHFPLFAAFRVPE
                     TWMSAVALVLAVLAGLGLAESMRRDRDAKDDARRSRLVYRVAGVAVVVTAVLALWGDS
                     FLSFERAGEYDGFVAQLQQARPDLSADDPQVQRAITQEIARYKAARVDAFVSDARRTA
                     LFVLVAGLLLVLARRRRLPGWVAALGLVVLVAFDLVGVGRRYVNGDVLVDAQEPGDLV
                     ATYAFDRYIMDRRDEAGGDGSFRVLSLEGDPTVTSRPAAHYESLGGYHGAKLRRYQDF
                     LESMLFDERGRLSMTALRMANVRYVAASRPPAAMESEYVDDQTGFSVFRVPEALPRAW
                     LVDTVTVIPDPDAQWARMAEPEFDPATEALLAADVGLAPAPRDSASIVSVRMESYEAE
                     SIRWTVETDRPRLLVVSEVFYPAGWTARIDGVAAEIHPANHAFRGVVVPGGRHEVTMT
                     FEPRTRRLGVAVAGASTAAVYGGLVLLLVLAWLKRRKGMQA"
     gene            5325..6629
                     /locus_tag="AC16YU_08270"
     CDS             5325..6629
                     /locus_tag="AC16YU_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6065259.1"
                     /translation="MKRVLIISYYFPPAGGSAVQRILAMARHLPAYGWESVILTADPD
                     SASWPTTDPGLVRRLPEDLEVHRVAGPDPYVLYRRWTGRSPSIGFADGRAPGLRERVA
                     RWIRANLFLPDARIGWSRRAGRMAQDLVRSRGIDVVLTSGPPHSVHLAGLAASRATGC
                     PQVVDLRDSWPDPTYRHHLPTTRVARRRDTRMRGDVLRHASEIVVVSAPMADELPPLG
                     DVPVRVIGNGYEDDDFREAERMRHEGFELLHAGNLPAERNPEALWTAIAGLRDSGGIS
                     GLRVRLLGRVDRRVLESVRSCGLETIVTWEESLPHDEAIDRMVSADALLLSVNRVPGA
                     EGIVTGKVYEYVASGRPVVGIGPKGGRAEHVLARSGAGRLFDFDDHEGIRAHLLTLYD
                     AWAAGEPVRGASAESAAPFSRRARTRELAGVLDRAAAGGGRS"
     gene            6656..7714
                     /gene="wecB"
                     /locus_tag="AC16YU_08275"
     CDS             6656..7714
                     /gene="wecB"
                     /locus_tag="AC16YU_08275"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006092605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR6065260.1"
                     /translation="MYVVSIVGARPQFVKASMVSRELMLRGAREELVHTGQHYDHGMS
                     AVFFEELGIPEPSRNLGVGSGTHAAQTGGMMVGIERLLLDDRPDAVLVYGDTNSTLAG
                     ALAAAKLRVPVAHVEAGLRSFNRAMPEEINRILTDHVSDLLFCPTETAARNLAVEGVT
                     SGVVRTGDVMLEATRHFADRAAERAPLSGLTGHGAGEYAVATVHRAGNVDSPAVLRSI
                     LDGLGRTGLPVILPLHPRTAGRLESMKIPENVEIRDPVGYLAMLTLVRHARCVLTDSG
                     GLQKEALWLSVPCITLRDETEWVETLQGGWNRLVGADPDRIRDAARERPVGPPPAFGS
                     MPEAPASVLIADALVRAI"
     gene            7731..8921
                     /locus_tag="AC16YU_08280"
     CDS             7731..8921
                     /locus_tag="AC16YU_08280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6065261.1"
                     /translation="MRILMVLDKPFPPDLRVENEARSLVGAGHEVRVLSVGPDDRARE
                     EEVFPGVFVRREVRSQSLVNRIRALAATWPRTDRFYAGIIEEAHRDRPFDAVHVHDLY
                     LAGGGIRAARRLGIPCIVDLHEHWTRVLDDYEWSSGFPRRWIVGRRRWRRYERRRLER
                     ADAIAVVGEDMAAHYAYVGRPVIPVPNYVSLEGYGALHPGSIVPGLAVPSILYAGGLV
                     PNRGLDTAIRAMPRILRAVGPVQLDIVGEGVDRSALERLAAGLGMGDVVRFAGWVPLE
                     QIPGRVLQARVCINLLRRTGQTEVALSHKLFQYMLAERPVVVTDCVAMARVVRDAGAG
                     IVVPDRDVEAYAAAVIRLLTHPEEAARFGRQGRQAVLDRYNWDEAVKPLLAWYASRDP
                     GATR"
     gene            9106..9390
                     /gene="eutM"
                     /locus_tag="AC16YU_08285"
     CDS             9106..9390
                     /gene="eutM"
                     /locus_tag="AC16YU_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HIR6065262.1"
                     /translation="MSDDYGIALGMIETRGLVGAIEAADAMVKAARVHLVGKETIGGG
                     YVTVMVRGDVGAVKAATDAGAAAAERVGELVSVHVIPRPHGDVETILPKG"
     gene            9421..9891
                     /locus_tag="AC16YU_08290"
     CDS             9421..9891
                     /locus_tag="AC16YU_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR6065263.1"
                     /translation="MNGLDQDALGIVETRGLVAAIEAADAMLKAARVRLVRVEKTVPA
                     LMSVQILGETAAVRAAVDAGRLAAEKVGTVVSVHVIPRPADDTVRMQRLAPPPVAPAS
                     RKTASRAKGVDPESMTVRELRALARTTPGLPIQGRAIARASKAELVDAFSRLSG"
     gene            9948..>10417
                     /locus_tag="AC16YU_08295"
     CDS             9948..>10417
                     /locus_tag="AC16YU_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065264.1"
                     /translation="MATSESGRISTWLLKRARAIVLHAQRRDDGVERPPRRRGLVITV
                     CILASVLLWFTFAMRETYTRVLEFPTEVRNLPADEALIELPPRLVRAQVEGEGIQLIR
                     LYYDPPLIPIDAASESVDLTVLAPDVVKNVRLDAVMPRTVVVYKEPRIQRRVPVR"
BASE COUNT         1425 a         3542 c         3802 g         1648 t
ORIGIN      
        1 ggaggcggcc cgcgtgctgg ccggtctcgc ggttgtcggg caggggccgg accggaccgt
       61 gctcttcgcg ctcttcgcgg ggtcccggca ggacgacagc ggagcccggg cgtggctcgc
      121 gaatcccacg tggccggcag acgcgacccg cgaggtggtc ctcgtcggcg cgccggaccc
      181 gggtccgttt ccgggacgtg cgtatccggt acgaacgatc gggtcggacg ccgtcgcacc
      241 cggatccggg agcctcgtcg accgagcgtc cgcccaggcc gctgcgctct cccgcgaggt
      301 gctgctcgcc gcctcgggca tgatctggca ccgggacggg gcgtcgcgcg ttccggacga
      361 cgggccgtga gtccttccac ccggacgacg aatgattcct tcctggcttc ccgatacggt
      421 gacctccgct cccgaccgca tggtcgagcg tgcgctgctc tggggcctgg agggtctgga
      481 actccgtacg gtcggtgggc cggatgagcg cgttccgtac gtgaacgagg cccggattcg
      541 ccgtctgctc acggaatcgg agctcgccgc gacggccgtc gtgcccggcc tgttcgaggc
      601 ggacgtcgac gaccgggcat cgtggctgaa cgacctcgcc ctgctggacg agacgctcgc
      661 gttctgcagg aggatcgggt cccggatcgt ggtcgtctcg tcgttccgcc gaggggcggg
      721 ggaggaccgg gtcgcggaag cgctgcgccg cgccggggac caggccgccg ggcgaaacat
      781 ggcgcttgcc gtgctgaacg agcccggtat ggggtgcgag accggagcgg aactcggggc
      841 cgttctcggg gctgccgccc atcccgccgt gcgcgcggcg tgggaccccg cctgcgcgtc
      901 ggccgccggc gaggaaccgg ccgaaggcgt ccgactcgtc gcgcggcacg tggcgctggt
      961 ccgccatgcc gtgggcggat cctccgccgt cgactgggac gcacagttcg cggccctcac
     1021 ggagcgggcc ttcgacggac cggtgagcct ggtgcttccc cccggcgcgg tccgcgccga
     1081 gggtctcgcg gcggcgaccg gaatgatcgg acgcatgcgg gcggtggacg tgcggcgatg
     1141 actcagagcc cgaggcctac gtagacatgg ctccccggtg gcgccactcc ggcaccgggg
     1201 agccgggcgg ctcctcctcc aggattccgg tgagtccaac gacgccacgg acgccggcaa
     1261 taagccgcgt cccccacgcc ggaatcgtga cctcgactcc gaagccggcg gcgacgtcgg
     1321 tgcgccggac cccggagtac gagtccgaga accacgatcc gccggcaagg gggcgatacc
     1381 ggagggtgga ttccgccgtc catccgaaca cgggacccgc gaacacccgg ggctgcaccg
     1441 tgcctccgag cgggacgtcc aggccggcca tgatcccgaa ttcgagggtc gtcagctcga
     1501 gcaccacgtc gacctcctgg tcggccacga ccccgtcgaa ggccgcgcca cgggtcaggt
     1561 agtggagctc cggccgaacg gagaagcccg accgaaaagg aaacgagacg cccacgccgg
     1621 ccgagagccg ggcgatcgga tcgaagtgct ccggatcggg gccggattcg gacagcgtga
     1681 acgccgtgac gcccagcgtc aaccgggcat cgccctggcc ggccgcaggc cgggctgcga
     1741 ccaccgccag cgcgaaggcc gtgccggcga gcacggcgcg aacccggagc gggggtatac
     1801 ggttcatggc cattccagcg gatggagccg taaaggtagc ggcgccgggc gtgtcgtacc
     1861 gacgggattc cctggccggt agcggcggaa ccccgaaccg tgccgccggc cgcccccggt
     1921 tgcccctgaa ccctgccatg aacccacacc cgaccgacga atccggtctc caggtgccga
     1981 tcacgccctg gacgcggctg cacttcgcgt ggtccgtcgc gtgggtcgcg atcctgacgg
     2041 cggttttcgc cgtgggctac ctcgtgaccc ggctggcccg ccccacggcg gcgcacttcg
     2101 cgtggtggtc ccggcactgg ggccggtcga tgatgcggat gatgggcatc cgggtggaga
     2161 cctcggggct gcaccatatc gagccgggga ctccgtacgt cttcgtggcc aaccaccaga
     2221 acggcctgga cattccggtc gtggccgact gcctgcccgt gccgttcgga ttcgtggcca
     2281 aggccgagct ggagcgcgtc ccgttcctgg gcgccgccct gcgctactcg ccgtccgtgt
     2341 tcgtggaccg gacggacggg cgtcgcacgc tggagagcat gcgttccgcg ggagaggaga
     2401 tccggtccgg cagctcggtg ctcgtctttc cggaaggggc gcgctcgtat gacgggcgca
     2461 tgggcccgtt ccagaagggg gccttcctgc tggccgtcga ggcgggggtg ccgatcgtcc
     2521 ccctgacgat cgtggacggc cccggcgtct tcaacgagaa gcggcgcgtg tcgcgacccg
     2581 gcgtcatcca cgtcgtggtc gggcaggcca tagcgctgga gggggtatcg cggcgggaca
     2641 tcccgtcgct catggagcgc acggcccgtg cgatcgcgga accgctgccg gaggattgga
     2701 ggaacccccg aaccggactc ccgtaacggc ggcccgattc gtactctgct gcagccacac
     2761 ctccgctgcg cccgggcccg gaccgggagc gagaccgact gaacacgcat ggtcgcccgc
     2821 aaatcccccc gccgagcggc aacggacgct cccgagatca agccctcctg gttcgaggcg
     2881 tgggaccgga tatcgccggg acggcggcac ggcgtatgcg ccgccgcact cctcgtcatc
     2941 ggcgtcacgt tcatggcgcc ggtccttttc ggcgacggcc gcctcgtggg gggtgacatc
     3001 gtccactggt cgggaatgtc ccgggcgctc ctcgaaacgg aggaggccac cgggcaccgg
     3061 ccgctctggg cggtccggcc gttcgcgggc atgccggcct acatgatctc gtacgacctc
     3121 gtcgttccgc agatcgacct cgtggccggc cttctcaggc gcctgatgtg gccgctgtcc
     3181 catctcctga tcctcttcgc gggaacgtac ctgctcacgt tcctgctgac tcgcgaccgg
     3241 ttggccggcg tgctggccgc cgtcgctttc ggactcacga cgtatctgcc agtccttctc
     3301 atcgccggac acaattcgaa gttcatcgcg ctgtcgttcc tgccgtggct gctcctggcg
     3361 ttcgtacacg cgctgcggcg tccgggcctg gtttcgggcc tgctcttcgc cgtcgcggtc
     3421 gggctgaacc tgagggcggg gcacgtgcag atcacgtact acggcgcctt catcatcggc
     3481 gtatggtggc tggtcgaggc ggtgtcggcc ctccggcagg gccggctgcg cgagctggga
     3541 aggtcgactc tctggctggc cgtgggcggg ctgctcggcg tgatgatggt ggccgacccc
     3601 tacctggtcc aggcggagta cagggagttc acgatccggg gcgcgtcctc gggcggcggc
     3661 gagggcggaa tggcctggga ctacgcgatg gcctggagcc agggcgtggg cgagctgctc
     3721 acgctcgtca tcgcggacgc gttcggcggt ggaggggcca cgtactgggg ccccaagacc
     3781 ttcacggcgg ggccgcacta ttcggggggg atagtcatca tgctggccgc gatcgccctc
     3841 gcgcggagcc gccagaggac cgtggcggcg ctggcggtcg ccggggtcct gatgatcggt
     3901 tttgcgctcg gcgagaacct ggccgtcctc aatcgcctga tgttcgagca tttcccgctg
     3961 ttcgcggcgt tccgcgttcc agagacgtgg atgtcggcgg ttgccctcgt gctcgccgtc
     4021 ctggcgggac tggggctggc cgagtccatg aggcgggacc gcgacgcgaa ggatgacgcc
     4081 agacgctcgc gcctggtcta ccgtgttgcc ggggtggcgg tggtcgtcac ggcagtgctg
     4141 gccctgtggg gggactcctt cctttcgttc gagcgggccg gcgagtacga cgggttcgtg
     4201 gcgcagctgc agcaggcgcg ccccgacctg tcggccgatg atccccaggt gcagcgcgca
     4261 atcacgcagg aaatcgcgcg gtacaaggcg gcccgggtgg acgcattcgt ctcggacgcc
     4321 cggcgcacgg ctcttttcgt gctggtggcg gggctgctgc tggtccttgc ccggcggcgc
     4381 aggcttcccg ggtgggtcgc ggccctcggc ctggtggtac tcgtcgcctt cgacctcgtc
     4441 ggcgtcggga ggcgatacgt gaacggggat gtgctcgtcg acgcgcagga gccgggggac
     4501 ctcgtcgcga cgtacgcctt cgaccgctac atcatggacc ggcgggacga agcgggcggc
     4561 gacggcagct tcagggtgct ttccctggag ggcgatccca ccgtgacttc ccgcccggcg
     4621 gcgcactacg agagcctggg cggctatcac ggtgcgaagc tccgtcgata ccaggacttc
     4681 ctcgagtcga tgctgttcga cgagcggggg aggctcagca tgaccgcgct gcgcatggcg
     4741 aacgtacgct acgtggcggc ctcccggccg cccgcggcca tggaatcgga gtacgtggac
     4801 gaccagaccg gcttctcggt gttccgggtt ccggaggccc tgccccgggc ctggctcgtc
     4861 gataccgtga cggtgatccc cgatccggat gcccagtggg cgaggatggc cgagccggaa
     4921 ttcgaccccg cgacggaggc cctgctcgcc gccgatgtgg gactcgcgcc cgctccccgc
     4981 gactcggctt ccatcgtgtc ggtccgcatg gaatcgtacg aggcggaatc gatccggtgg
     5041 accgtcgaga cggatcgacc gcgcctcctc gtggtcagcg aggtctttta cccggcagga
     5101 tggaccgccc ggatcgacgg agtggcggcg gagatccatc ccgcgaacca tgccttccgc
     5161 ggcgtggtcg tacccggagg gcgccacgag gtgaccatga cgttcgagcc ccggacgcgt
     5221 cggctgggcg tcgcggtggc cggagcatcc acggcggccg tgtacggagg ccttgtcctg
     5281 ctcctcgtac tcgcctggct caagcggagg aaggggatgc aggcgtgaag cgcgtcctga
     5341 tcatctccta ctattttccg ccggcggggg ggtcggcggt gcagcggatc ctcgccatgg
     5401 cgcgccacct tcccgcgtac ggttgggaat ccgtgatcct gacggcggac ccggacagcg
     5461 cctcctggcc caccacggat cccgggctgg tgcgtcggct tccggaagac ctggaggttc
     5521 accgggtcgc cggccccgat ccgtatgtcc tgtacaggcg ttggaccgga cgcagcccgt
     5581 cgatcgggtt cgccgacggc agggcgcccg gcctccggga gcgggtagcc cggtggattc
     5641 gggcgaacct ctttctgccc gacgcccgga tcggctggtc caggcgcgcc ggccggatgg
     5701 cacaggatct cgtgcgctcg cgcggaatcg acgtcgtgct cacgtccggc cccccgcact
     5761 cggtgcacct cgccgggctc gccgcctcca gggccacggg gtgcccacag gtggtcgacc
     5821 tgcgggactc gtggccggac cccacgtatc ggcatcatct cccgacgacc cgtgtggcga
     5881 ggcggaggga cacgcgcatg cggggggacg tgcttcggca tgcatcggag atcgtcgtcg
     5941 tgtccgctcc catggccgac gagctgccgc cgctcgggga cgtgccggtc cgggtcatcg
     6001 ggaacgggta cgaggacgac gatttccggg aggcggaacg gatgcggcat gaaggcttcg
     6061 agctgctcca tgccgggaat ctcccggccg agcggaatcc ggaagccctg tggacggcga
     6121 tcgccggact ccgcgattcg gggggtattt ccggcctgcg agtgcggctg ctcgggcggg
     6181 tcgaccgccg ggtgctcgaa tcggtacggt cgtgcggcct ggagaccatc gtgacctggg
     6241 aagagagcct tccgcacgac gaggcgatcg accggatggt ctcggccgat gcgcttctcc
     6301 tgtcggtgaa ccgcgtgccc ggcgccgaag gcatcgtcac cggcaaggtg tacgagtacg
     6361 tcgcctcggg acggccggtg gtcggaatcg gtccgaaggg cggcagggcc gagcacgtgc
     6421 tcgcccggag cggcgccggc cggctgttcg acttcgacga tcacgagggc atccgggcgc
     6481 acctgcttac cttgtacgac gcctgggcgg ccggcgagcc ggttcgaggc gcgtccgcgg
     6541 aatcggccgc ccccttcagt cggcgcgcga ggacacgcga actcgcgggc gtcctggacc
     6601 gggcggcggc gggcggcgga cgaagctgac ggagaccttt cccgggagac tgccgatgta
     6661 tgtcgtatcg atcgtaggag ccagaccgca attcgtgaag gcgtcgatgg tcagccgcga
     6721 gctcatgctc cgcggcgccc gcgaggagct cgtccacacg ggtcagcact acgaccacgg
     6781 catgagcgct gttttcttcg aggaactcgg catcccggag ccgtcacgga acctgggggt
     6841 gggttcgggc acccatgccg cgcagaccgg cggcatgatg gtcggaatcg agcgacttct
     6901 cctcgacgac cgaccggatg ccgtcctggt ctatggggac accaactcga cgctcgcagg
     6961 cgccctcgcg gcagccaagc tgcgcgtgcc ggtcgcgcac gtcgaggcag ggctgcgctc
     7021 cttcaaccgg gcgatgcccg aggagatcaa ccggatcctc acggaccacg tttccgacct
     7081 cctgttctgt ccgacggaga cggccgccag gaacctcgcg gtcgaaggcg tcacgagcgg
     7141 cgtcgtccga accggcgacg tcatgctcga ggccacgcgc cacttcgccg accgggccgc
     7201 ggagcgggcg ccgctgtccg gcctgaccgg gcacggcgcc ggggagtacg ccgtcgcgac
     7261 ggttcaccgg gcggggaacg tggactcccc cgccgtgctt cgatccatcc tggacggcct
     7321 gggccggacg ggactccccg tcatcctgcc cctgcatccc cgtaccgccg gtcgtctgga
     7381 gagcatgaag attccggaga acgtggagat ccgcgatccg gtgggctatc tggccatgct
     7441 caccctcgtg aggcacgccc gctgcgtgct cacggactcc ggtgggctgc agaaagaggc
     7501 gctctggctc tcggtgccgt gcatcacgct ccgcgacgag accgagtggg tcgaaacgct
     7561 gcagggcggg tggaaccgcc tggtcggggc cgatcccgac cggatacggg acgcggcgcg
     7621 cgagcggccg gtgggtccgc cgccggcgtt cggctccatg cccgaagcgc cggcctcggt
     7681 gctgatcgcc gatgccctgg tccgggcgat ctgaagccgc gaggtcctgc atgcgcatcc
     7741 tgatggtcct ggacaagcca tttccccccg atcttcgggt ggagaacgag gcccgttcgc
     7801 ttgtgggtgc gggccacgag gtgcgcgtgc tgtccgtggg gccagacgat cgggcgaggg
     7861 aggaggaggt gtttcccggc gtcttcgttc ggagggaggt ccggtcgcag tccctcgtga
     7921 accggattcg ggcgctggcc gcgacctggc cccggaccga tcggttctac gcggggatca
     7981 tcgaggaagc gcaccgtgac cgcccgttcg atgcggtgca tgtgcacgac ctgtacctgg
     8041 ccgggggcgg gatccgcgcg gcccggcgcc tgggcattcc gtgcatcgtc gaccttcacg
     8101 agcactggac gcgggtgctg gacgattacg aatggagcag cggttttccg cgccgatgga
     8161 tcgtgggccg tcgacgctgg cggcgatacg agcggcgtcg cctggagcga gcggacgcaa
     8221 tcgcggtcgt gggggaggac atggccgccc actacgctta cgtggggcgg cccgtgattc
     8281 cggtgcccaa ctatgtcagt ctggaggggt atggtgctct gcacccgggt tcgatcgtgc
     8341 ctggccttgc cgtgccgtcg atcctgtacg ctggcggtct cgttccgaac cgggggctgg
     8401 atacggcaat ccgggccatg cccaggatcc tgcgggccgt ggggcccgtg cagttggaca
     8461 tcgttggaga gggcgtggac cgatccgcgt tggagcgtct cgccgccggg ctcgggatgg
     8521 gggatgtcgt gcgcttcgcg ggctgggtgc ccctcgagca gattcctggt cgggtgctgc
     8581 aggcccgggt gtgcatcaac ctgctccgcc ggaccggcca gacggaggtc gccctttcgc
     8641 acaagctgtt ccagtacatg ctcgcggagc gccccgtcgt ggtcacggac tgcgtggcca
     8701 tggcccgggt ggtgcgtgac gcgggtgccg gaatcgtggt gccggatcgc gacgtggagg
     8761 cctacgccgc tgcagtcatc cggctgttga cccatcccga ggaggcggcc aggttcggcc
     8821 gccagggccg gcaggccgtt ctcgaccgct acaactggga cgaggccgtg aagcccctgc
     8881 tggcctggta cgcatcccgc gatccgggag ccacgcggta acccggctcc cggaaccgcc
     8941 gggtcgcgct cccgcctccg ccggccggtt tgacaacccg tccgggcggc gtctatattc
     9001 gcccgaccgt ctccgtgctc cggctccgtg ctccgcgatc ccgggaccgg gggacagccc
     9061 ttccggcggc gcgatcaggc cgcccgcact caatgattct ccgccatgtc tgacgactac
     9121 ggaatagccc tcgggatgat cgagactcgc ggtctcgtcg gcgcgatcga agcggccgac
     9181 gccatggtca aggccgcgcg cgttcacctc gtgggcaagg agaccatcgg cggcggctat
     9241 gtcaccgtca tggtccgtgg ggacgtgggg gccgtcaagg ccgccaccga tgccggggcg
     9301 gccgccgccg agcgcgtcgg tgagctggtc tccgtccatg tcattccgcg gccgcacggc
     9361 gacgtcgaga cgattcttcc gaagggctga tccggacggc ctcgccaagc ggagagcgcc
     9421 atgaacggac tcgaccagga cgccctcggg atcgtcgaaa cccggggact cgtcgcggcg
     9481 atcgaagccg cggacgcgat gctcaaggcc gcgcgcgtcc gcctggttcg ggtggagaag
     9541 accgtgccgg ccctgatgtc cgtgcagatc ctgggcgaga cggccgccgt ccgggccgcc
     9601 gtcgacgcgg gacgcctggc cgccgaaaag gtcggaaccg tggtatcggt gcacgtcatc
     9661 ccgcgtccgg ccgacgacac ggtgcgcatg cagcgccttg cgccgccgcc tgtggcgccg
     9721 gcctcccgga agacggcatc acgcgcgaag ggagtcgatc cggagtccat gaccgtccgc
     9781 gagctccgcg cgctggcccg cacgacgccc gggcttccga tccagggacg ggcgatcgcc
     9841 cgtgcgagca aggcggagct cgtcgacgca ttcagccgtc tttccggatg acggggatcg
     9901 cccttggcgc ccgggcgtaa cgttccgcga cccaccgagc ccgtccagtg gcgacttcag
     9961 aatccggcag aatctcgacc tggctgctca agcgggcgag agccatcgtc ctgcacgccc
    10021 agcgccggga cgacggcgtc gagcgacctc ctcgccgcag gggcctggtg atcaccgtct
    10081 gcatcctcgc gtcggtgctc ctgtggttca cgttcgccat gcgcgagacg tacacccgcg
    10141 tcctggaatt cccgaccgag gtccggaacc tgccggccga cgaggcgctg atcgagcttc
    10201 cgccgcgcct ggtgcgcgcc caggtcgagg gcgagggaat ccaattgatc cgcctgtact
    10261 acgatccgcc cttgatcccg atcgatgcgg cgagcgagtc ggtggacctc acggtgctag
    10321 cgcccgacgt ggtcaagaac gtccgcctgg acgcggtgat gccccggacg gtcgtcgtat
    10381 acaaggagcc gcggatccag cggcgggtgc ccgtccg
//
LOCUS       DBNHQZ010000309         4330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_363527, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000309 DBNHQZ010000000
VERSION     DBNHQZ010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_363527"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="AC16YU_08300"
     CDS             <1..181
                     /locus_tag="AC16YU_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065265.1"
                     /translation="FPAALLGLLSLLEPIGASVFAAVLFGEVPGRLAIGGMAMILVAV
                     GLTVVRRPRAVPVTD"
     gene            complement(257..1288)
                     /locus_tag="AC16YU_08305"
     CDS             complement(257..1288)
                     /locus_tag="AC16YU_08305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6065266.1"
                     /translation="MTDSPTVSVIIVSWNALPLLRKCLPSVAATDWPGIEVVLADNGS
                     DDGSAEWVEAQLPGVRIVRHPENWGFCRGNNEAIRQTTSEFVVLLNNDVEVTAGWLAP
                     LVERMQGDPTIGAVQPKLLAYDDRGRFEYAGAGGGYLDRWGYPFARGRIFFTLEEDRG
                     QYDRSIDVFWGSGAALMLRRDALGQVGLLDERFGFHMEEIDLCWRLHRAGWRVVAEPA
                     SVVYHIGGGSLPRGHPRKTYLNFRNSLLMLYKNLSPRAWRRVLPVRAALDLVAALRSL
                     ATGAPRDAWAIVRAYADAHRMKRAYREKRAGEGEPFPAYRGSIVVDYFLRGRRRFSDL
                     PADRFDMQG"
     gene            complement(1285..1941)
                     /locus_tag="AC16YU_08310"
     CDS             complement(1285..1941)
                     /locus_tag="AC16YU_08310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6065267.1"
                     /translation="MPLESGRPGRGGPDFGIESVLWRGGHAAVAGVDEAGRGCLAGPV
                     VAAAVILDPDRDVDGLDDSKRLSPAARTRLAALIRERAVAVSVGRSSPAEIDRRNILQ
                     ASLEAMRRAVDGLGVAASFVLVDGDRMWPRPTVPWRTVVKGDARSRTIAAASIIAKTE
                     RDLEMERLAERHPGYGWERNRGYPTPDHFEALRLLGPSPEHRRSFRLRTGELQLFPGP
                     "
     gene            complement(1945..3555)
                     /locus_tag="AC16YU_08315"
     CDS             complement(1945..3555)
                     /locus_tag="AC16YU_08315"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR6065268.1"
                     /translation="MTGVLLAGAVALLFALWRAGRRLRFFLHVLQLEGYRAGALLAWT
                     VRRPTDVLFRLSHALGVVLVAAALLWTGPGAQVILLLLWAAAFASSRRYRRDRPKKPL
                     AATPRLRRLAAVAALPPLGLAAWGLVAALAPGGPPAVAPFVAGLWLADLGAPLWAALA
                     GVLLSPVERRIHEGFKARARARLAERPDLVVVAVTGSYGKTSVKFMIAEMLARRYNVL
                     ATPGSFNTPMGICRVINDQLRPEHQVMVLEMGIRHPGDIAELCAIARPHVAVITGVGV
                     AHLESMGSEQAIADEKGSLLDYLLPGGTAVLNADDAWYGEHRARATGPVVGVSASGLE
                     TADLRATGVRFGPDGTACTVTGDGRPLEVTLRLLGRHNVGNMLLGLAVARSLGVRPRQ
                     AALAAAGLEPVPHRLALRKEGALLVLDDAFNSNPVGARSALEVLGQFPGRRFVVTPGM
                     IELGEREEAENEAFGAAMQGRVDEVYLVGPGRTRAIARGLRESGFPEERVHVVHTLFE
                     ARDAVRRSATDGDVVLYENDLPDQYTEA"
     gene            complement(3552..>4330)
                     /locus_tag="AC16YU_08320"
     CDS             complement(3552..>4330)
                     /locus_tag="AC16YU_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6065269.1"
                     /translation="RHGPLSVTASGPPDGKPVVLLHGWGSSAALMEPVARGLADRWRI
                     YNVDLPGHGTAPVPAEAMDLDDHAEAVAALVREVAPGGAALVGHSNGGRIAMRLATRP
                     ATAALAKRLVLVSPSGIRRKRTLRYAIRRSLASALKAPFSLLPGRLRELGLDWLRHSL
                     VWRMLGSSDYRNVEGVMRQIFVSLVNTYVEDDLPRLAVPVLVFRGTRDEAVTADQVRR
                     LVSLVPDAGLVQLPGAGHYGFLDAPEPFLAGTRHFLEAWA"
BASE COUNT          618 a         1562 c         1610 g          540 t
ORIGIN      
        1 tttcccggcg gcgctcctgg ggctgctgag cctgctcgag ccgatcgggg cgtccgtgtt
       61 cgccgccgtg ctcttcggtg aggttccggg ccgcctcgcg atcgggggca tggccatgat
      121 tctcgtggcc gtcggcctga ccgtggtccg gagaccgaga gcggtgccgg tcaccgactg
      181 atcgcgccgg gtgggccggc cgcgggccgc ggatcgctgg tcgggagccg accgccgacg
      241 ggcagccggg cggggctcac ccctgcatgt cgaagcggtc tgccggaagg tccgagaagc
      301 gccgtcgtcc acgtaggaaa tagtcgacga cgatcgagcc ccggtaggcc gggaacggct
      361 cgccctcgcc agcgcgcttc tcccggtacg cccgcttcat gcgatgcgca tcggcgtagg
      421 cccgtacgat cgcccaggcg tcccggggtg cgcccgtcgc gagcgaacgc agggcggcca
      481 cgaggtcgag cgcggcccgc acggggagca cgcgccgcca cgcgcgcggc gagaggttct
      541 tgtacagcat caggagcgag ttgcggaagt tcaggtaggt cttccgcgga tgcccccggg
      601 gcagcgaccc cccgccgatg tggtagacga cggacgccgg ctccgccacc acccgccagc
      661 ccgcccggtg caggcgccag cagaggtcga tctcctccat gtggaagccg aaccgctcgt
      721 ccagcagccc cacctggccg agcgcgtccc gccgcagcat gagggccgcg ccggagcccc
      781 agaagacgtc gatcgagcgg tcgtactgtc cccggtcctc ttcgagggtg aagaagatcc
      841 gtccgcgcgc gaacggatat ccccaccggt ccaggtaccc gccgccggcg cccgcgtact
      901 cgaaccggcc gcgatcgtcg tacgcgagga gcttgggctg cacggcgccg atcgtcggat
      961 cgccctgcat gcgctcgacg agcggcgcga gccatccggc cgtgacctcg acgtcgttgt
     1021 tcaggaggac gacgaattcg ctcgtcgtct gccggatcgc ctcgttgttg ccccggcaga
     1081 aaccccagtt ctccgggtgg cgcacgatgc gcacgcccgg gagctgcgcc tcgacccatt
     1141 ccgccgatcc gtcgtccgat ccgttgtcgg cgagcaccac ctcgatgccg ggccaatcgg
     1201 tcgcggcgac gctcggaagg cacttcctga ggagcggaag ggcgttccag gacacgatga
     1261 tgacgctgac ggtggggctg tcggtcacgg gccgggaaag agctggagct ctcccgtccg
     1321 gaggcggaag ctccggcggt gctcgggtga aggccccagc aggcgcaggg cctcgaagtg
     1381 gtcgggcgtg ggatagccgc ggttgcgctc ccagccgtag ccggggtgac gctcggcgag
     1441 gcgctccatc tcgaggtcgc gttccgtctt cgcgatgatg gacgccgccg cgatcgtgcg
     1501 gctcctggcg tcgcccttca ccaccgtcct ccacgggacg gtcgggcgcg gccacatgcg
     1561 gtcgccgtcc acgagcacga acgatgccgc cacgcccagg ccgtccaccg cgcgccgcat
     1621 cgcctcgagc gaggcctgca ggatgttgcg ccggtcgatc tcggcgggcg atgagcggcc
     1681 gaccgagacc gcgacggccc gctcgcggat gagggcggcc agccgggtgc gggcggcggg
     1741 ggagaggcgc ttgctgtcgt cgaggccgtc cacgtcacgg tccgggtcca ggatgacggc
     1801 cgccgccacc accgggccgg ccaggcagcc gcgcccggcc tcgtccacgc ccgcgacggc
     1861 cgcgtggccg ccgcgccaga gcacggattc gatcccgaag tcggggccgc cccgcccggg
     1921 ccggccggat tcgaggggca tgtgtcaggc ctccgtgtac tggtcgggca ggtcattctc
     1981 gtagaggacc acgtcgccgt ccgtggcgct gcggcgcacc gcgtcgcggg cctcgaacag
     2041 ggtgtggacc acgtgcacgc gctcctccgg gaagcccgac tcgcgcagcc ctcgggcgat
     2101 ggcgcgcgtg cgcccggggc cgacgaggta cacctcgtcc acccgtccct gcatcgcggc
     2161 gccgaaggcc tcgttctcgg cctcctcacg ctcgccgagc tcgatcatcc ccggggtcac
     2221 cacgaaccgt cggccgggga actgtccgag cacctcgagg gcgcttcgcg cgcccacggg
     2281 attcgagttg aaggcgtcgt ccagcacgag gagggcgccc tccttcctca gggcgaggcg
     2341 gtgcgggacg ggctcgaggc ccgccgcggc aagcgccgcc tgccgcgggc gtacgcccag
     2401 ggaccgggcg acggccagac ccaggagcat gttgcccacg ttgtggcgtc ccaggagccg
     2461 tagcgtgacc tcgagcgggc ggccgtcgcc ggtcacggtg caggccgtgc catcggggcc
     2521 gaagcggacg ccggtggccc gcaggtccgc ggtctccagg cccgaggccg aaacgccgac
     2581 gacggggccc gtggcccggg cccggtgctc cccgtaccat gcgtcgtccg cgttcagcac
     2641 ggccgttccc ccgggaagca ggtagtcgag gaggctgccc ttctcgtcgg cgatcgcctg
     2701 ctccgatccc atggactcga ggtgcgcgac ccccacgccc gtgatgacgg ccacgtgcgg
     2761 ccgggcgatg gcgcacagct cggcaatgtc gcccggatgg cggatgccca tttcgagcac
     2821 catcacctgg tgctccgggc ggagctggtc gttgatcacg cggcagatgc ccatcggcgt
     2881 gttgaacgag cccggcgtcg ccagcacgtt gtaccggcgc gcgagcatct cggcgatcat
     2941 gaatttgacg ctggtcttgc cgtaggagcc ggtgaccgcc acgaccacga ggtcgggccg
     3001 ctccgcgagc cgggcccggg cgcgggcctt gaagccctcg tggatccgcc gctcgacggg
     3061 cgagagcagc acgccggcga gggccgccca gagcggcgcg cccaggtcag cgagccacag
     3121 gccggccacg aacggggcca cggccggcgg cccgcctggc gcgagggcgg cgacgaggcc
     3181 ccacgcggcg aggccgaggg gcgggagcgc ggccaccgcg gcgaggcgcc tgaggcgcgg
     3241 cgtggccgcg agcggcttct tgggccggtc ccggcgatag cggcgcgacg acgcgaacgc
     3301 ggcggcccac agaaggagaa ggatgacctg cgctccgggg cccgtccaca ggagcgccgc
     3361 ggccacgagc accacgccga gcgcgtgcga aaggcggaag aggacgtccg tcggacggcg
     3421 gacggtccac gcgagcaggg cgccggcacg gtagccctcc agctgcagca cgtgcaggaa
     3481 gaagcgcagt cgccgtccgg cgcgccacag ggcgaagagg agtgcgaccg ccccggcgag
     3541 gagaacgccc gtcatgccca ggcctccagg aagtgccggg tgccggccag gaagggctca
     3601 ggggcgtcca ggaagccata gtgcccggcg ccaggaagct gcacgagccc ggcatccggg
     3661 acgagcgata ccaggcgccg cacctggtcg gcggtcacgg cctcgtcgcg cgtgccgcgg
     3721 aacacgagaa ccggcaccgc gagacgcggg aggtcgtcct cgacgtacgt gttcacgaga
     3781 ctcacgaaga tctgccgcat taccccttcc acgttacggt aatcggacga cccgagcatc
     3841 cgccacacga gcgagtgccg gagccagtcc agcccgagct cccgcagcct ccccggaagc
     3901 agcgagaagg gcgccttgag agccgacgcg agcgacctgc ggatcgcgta gcgcagcgtg
     3961 cgcttcctgc ggatccccga cgggctcacc agcaccaggc gcttcgccag ggcggccgtc
     4021 gccggccggg tggccaggcg catggcgatg cgcccgccgt tcgagtggcc gacgagggcc
     4081 gcgccgcccg gggccacctc gcggacgagc gcggcgacgg cctcggcatg gtcgtccagg
     4141 tccatggcct ctgccgggac gggggcggtg ccgtgccccg gcaggtccac gttgtagatg
     4201 cgccaccggt ccgcgagtcc gcgggccacg ggctccatga gcgcggcgct cgatccccag
     4261 ccgtgcagga ggacgacggg cttgccgtcc ggcgggccgg acgccgtgac gctcagggga
     4321 ccgtgacgaa
//
LOCUS       DBNHQZ010000310         6413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3207169, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000310 DBNHQZ010000000
VERSION     DBNHQZ010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3207169"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1317)
                     /gene="mnmA"
                     /locus_tag="AC16YU_08325"
     CDS             complement(208..1317)
                     /gene="mnmA"
                     /locus_tag="AC16YU_08325"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6065270.1"
                     /translation="MSTHGRVLVAMSGGVDSSVAAVLLREEGYDVVGITMKTWDYAFS
                     GGRADRQIGCCTLESMNDARAVGIRHGFPHFIVDIREEFGDWVIDRFTSEYMAGRTPN
                     PCVLCNTHIKWAALLRRADALDCAWIATGHYARVWKDEASGRSVLRRGLDGNKDQSYA
                     LWGVDQEHLARSLFPLGDREKPEIRRMAAEFGLDHVADKPDSYEICFVPDNDYRRFLR
                     DRVPGLEERVGPGHLVLSDGRTVGMHEGYPFYTIGQRHGLGLALGWPAYVTAIDPDTN
                     TVTVGPRDELMRQTLVADGLNLVRYPDLREERPAVGKIRYRDEGAPCLVRQTGDDSLE
                     VSFAVPRSAITPGQAVVLYEGDDVLGGAWIRHVAY"
     gene            complement(1357..2010)
                     /locus_tag="AC16YU_08330"
     CDS             complement(1357..2010)
                     /locus_tag="AC16YU_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6065271.1"
                     /translation="MPKPYPDHEPLLTLKDAAERLGVHPATLRRWADNGDVQVRLTPG
                     GHRRFPLSEIERLGRTPEETRVGETARAEDMATRAITVTRTGIHEHHDERWMTDMDEK
                     EREEKRLLGRRLVGLMMQYLSREDGDGEELLAEAQAIGRLYARTSIQKGLELSEALRA
                     TMFFRDQIVESAVLLPESSRGRPDASTRVYRRINTFLNSVQLAIADVYDAQRGSADE"
     gene            2204..4261
                     /locus_tag="AC16YU_08335"
     CDS             2204..4261
                     /locus_tag="AC16YU_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6065272.1"
                     /translation="MSAGGQRADARQVGADSLWQVEMREVVVTATRGARTIRSVPIPT
                     QVLSARTVVEQGATRHSDLLAEHPGLVVTHDQFGSGIQLQGFDAAYTLILIDGEPVIG
                     REGGVVDLERLPVGDIERVEIVRGPSSSLYGSEALAGVINIITRTPRHGFSAEAGVRY
                     ETHGTSDLNASVEARSERVAGRLMVNRYGSDGYDLVPEVPGLTAPSFSDYTVSGHLDI
                     EPADGTTIDLHGRYARMRQISTLGFAEQGQEFTFEEDADRDDWSFGPLWEQRIRPGLK
                     LTTRLYAAGSGNSYTLLNRDLMEESSRTEFRQWYRKAETQLDAVLGTRHVVTTGGGYV
                     GETVRADRVQGGERSNSNGYVFGQHQWFLADGIQVHSSARFDWHTDYASRLSPKLAVM
                     VSPSSVLRFRASVGTGFKAPTFQQLYMDFTNPVAGYSVVGSSDVARALAEFERLGQID
                     YFLTDPSAIGSIRPENSVAFNLGTEWDVTRTLNLQMNLFRNNVEDLIEHQPVAAKPNG
                     QYVFTYVNLNRIYTQGLELEAAWDPTSRWGVAVGYQFLDARDRDVLDRIDEGSVYRRV
                     NGRDERLTRSDYGGLFNRSRHSGSLRLTFRSPGHGLTSTIRAIYRSRYGYGDLNGNLI
                     LDDDREYVPGFALVHLTVSKDLTPWITAQAGIKNLLDTTYPTLVPSLPGRLLFAGVNV
                     TIR"
     gene            4281..4901
                     /locus_tag="AC16YU_08340"
     CDS             4281..4901
                     /locus_tag="AC16YU_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HIR6065273.1"
                     /translation="MRTAPRLTLLLALVLTLGFAACDSDPNDGEGLPLNATVVENLPA
                     DPAVNIGPMGPVGNGHFTFFSLRENEIVTDSASTDWDLGFRATEIIVNGGVSGPGEGR
                     AQVREGLFEEITEAPESGWRIDNEEGHAIRTGSGNGWYNYNPQAMTVSPIPGRVLLVQ
                     TADGKYAKLRIISYYKDAPETPDIDSESRYYTFEYVFQPDGSRILE"
     gene            4993..5724
                     /locus_tag="AC16YU_08345"
     CDS             4993..5724
                     /locus_tag="AC16YU_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065274.1"
                     /translation="MPSPIHYLPILTTLVSIPFAIVLFRRWRAKPDALYLMWWMIGVL
                     TYMAGTVTESLTTLLGWQLPVFKAWYITGALLGGAPLAQGTIHLLFSRRTANILSAIL
                     VTFVAIAAVPVLLSPVDLTLVETYRLSGAVLEWQWVRAFSPFVNTYAVIFLVGGAAWS
                     AVRYAKQGETMRARMWGNIWIAVGAILPGIGGGMARAGIVEGLYVGELVGLILIWTGY
                     HVIAGDRSASIHENQQMAAEAARAA"
     gene            5780..6214
                     /gene="aroQ"
                     /locus_tag="AC16YU_08350"
     CDS             5780..6214
                     /gene="aroQ"
                     /locus_tag="AC16YU_08350"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6065275.1"
                     /translation="MKLLVLNGPNLNLLGTRETDVYGTGTLAELERALREEFAGESLE
                     FAQSNHEGELVDALQRAAREGFDGVVFNPAGYSHTSVALRDAVAAVDVPVVEVHISNV
                     YARERFRHVSRTAGAAVGVIAGLGLAGYSLAVRYLLLHASKS"
     gene            6231..>6413
                     /locus_tag="AC16YU_08355"
     CDS             6231..>6413
                     /locus_tag="AC16YU_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6065276.1"
                     /translation="MAGRIRQLASETAVYGVSSIFARAVNFLLFPFYSNVFAPDVYGP
                     IIVVYAAFVFLNILYQN"
BASE COUNT         1002 a         2282 c         2102 g         1027 t
ORIGIN      
        1 gccgaccagg ccgaagccgc gagggcgagc aggaagaagt agcgtgccgc ccccgagccg
       61 cggcggcgcc aggccatgac ggcgacggtc cccgtcgaga acgacgcgac ggcgtacgga
      121 aggaccaggt agagcgactg gtcggtcatg aggggtccgg cgacatcggt aagggcgata
      181 tcgggcagta tcggccgggc gcaggggtca gtaagccacg tgccggatcc acgcgcctcc
      241 gagcacgtcg tccccctcgt acagcacgac cgcctgtccg ggcgtgatcg cactccgcgg
      301 cacggcgaac gacacctcga ggctgtcgtc gcccgtctgc cggaccaggc agggcgcccc
      361 ctcgtcccgg taccggatct tccccaccgc gggccgctcc tcccgcaggt ccggataccg
      421 gaccaggttc agcccgtcgg ccaccagggt ctgccgcatc agctcgtccc gcggaccgac
      481 cgtgaccgtg ttggtgtccg ggtcgatggc cgtgacgtag gccggccagc ccagggcgag
      541 tcccaggccg tgccgctgcc cgatcgtgta gaagggatac ccctcgtgca tgcccacggt
      601 ccggccgtcc gacagcacga gatgacccgg gcccacgcgc tcctccagcc cgggcacccg
      661 gtcgcgcagg aaccgccggt agtcgttgtc cggcacgaag cagatctcgt acgaatccgg
      721 cttgtcggcc acgtggtcaa ggccgaactc cgcggccatg cggcggattt ccggcttttc
      781 ccgatccccg agcgggaaca gcgaccgggc caggtgctcc tggtcgaccc cccagagcgc
      841 gtagctctgg tccttgtttc cgtccagccc ccgccgaagg acgctcctgc cgctcgcctc
      901 gtccttccag acgcgcgcgt agtgccccgt cgcgatccac gcgcagtcga gcgcatccgc
      961 gcggcgcagc agggcggccc acttgatgtg ggtgttgcac agcacgcagg ggttcggcgt
     1021 gcggcccgcc atgtactcgg acgtgaaccg gtcgatcacc caatcgccga actcctcacg
     1081 gatgtccacg atgaagtgcg ggaacccgtg gcggatgccc acggcccggg cgtcgttcat
     1141 ggattcgagc gtgcagcatc cgatctgccg gtccgcccgg ccgccgctga aggcgtaatc
     1201 ccacgtcttc atcgtgatcc cgacgacgtc gtacccctct tccctgagca ggaccgccgc
     1261 gacggacgaa tccacgccgc cgctcatcgc caccaggacg cgtccgtggg tactcatggc
     1321 tccggtctcc gatcgcgaag cgcgccggtg cgcgacctat tcgtccgcgc tgccacgctg
     1381 ggcgtcgtag acgtccgcga tcgccagctg cacgctgttc agaaaggtat tgattcgccg
     1441 gtacacccgg gtgctcgcgt cgggccgtcc gcgggaggac tcgggaagca gaaccgccga
     1501 ttccacgatc tggtcccgga agaacatcgt cgcccggagc gcctccgaga gttccagccc
     1561 cttctggatg gacgtacggg cgtacaggcg cccgatcgcc tgggcctcgg ccaggagctc
     1621 ctccccgtcc ccgtcctccc gcgagaggta ctgcatcatc aggccgacga gccggcggcc
     1681 caggagacgc ttctcctctc gctccttctc gtccatgtcc gtcatccagc gctcgtcatg
     1741 gtgctcgtgt atgcccgtcc gcgtgacggt gatggcccgg gtcgccatgt cctccgccct
     1801 ggccgtttct ccgacgcggg tctcctccgg ggtgcgcccg aggcgctcga tctccgacag
     1861 cggaaaacga cggtgacccc cgggagtgag gcgcacctgc acgtccccgt tgtctgccca
     1921 ccggcggagc gtcgccggat ggacgcccag gcgctcggcc gcgtccttga gcgtcaggag
     1981 gggttcgtga tcggggtacg gcttgggcat gagaagaacg tgcgcggtct tttcaaaact
     2041 tgtgcaaacc tgaacaatcc tatacgaccc cttgtatatt cggcccagct tcaaacggca
     2101 gggcggccag tccatgcaag ctttctgctt caatatagca tccggtacgc cgaggccggc
     2161 aattcgtgcc ggcatgtgga cggtcttcgc aatcgcatcc tggatgtccg cgggggggca
     2221 acgcgcggac gcccggcagg tcggtgccga ttcgctgtgg caggtcgaga tgcgcgaagt
     2281 ggtggtgacc gccacccggg gggcgcgtac gatccgcagc gtacccatac cgacccaggt
     2341 gctctcggcc aggacggtcg tggagcaggg ggcaacccgg cactccgacc tcctggccga
     2401 gcaccccggg ctcgtcgtga cgcacgacca gttcggatcg ggaatccagc tccagggctt
     2461 cgacgcggcc tacacgctca tcctgatcga cggcgagccg gtgatcggcc gcgagggcgg
     2521 cgtcgtcgac ctggagcggc tgccggtcgg ggacatcgag cgggtcgaga tcgtccgggg
     2581 cccctcgtcg tcgctctacg gctcggaagc actggccggg gtgatcaaca tcatcacgag
     2641 aaccccgcgg cacggcttca gcgcggaggc gggcgtgcgg tacgagacgc acggcacgtc
     2701 ggacctcaac gcctcggtcg aggcgcgctc cgagcgcgtg gccggccgcc tgatggtcaa
     2761 ccggtacggc tccgacgggt acgacctggt gcccgaggtc ccgggcctga cagccccgtc
     2821 tttctcggac tacacggtat cggggcacct ggacatcgag ccggcggacg ggacgacgat
     2881 cgaccttcac gggcggtacg cccggatgcg ccagatcagc accctgggat tcgccgaaca
     2941 gggccaggag ttcaccttcg aggaggatgc ggaccgggac gactggagct tcggcccgct
     3001 gtgggagcag cgcatccggc ccgggctcaa gctcacgacc cggctatacg ccgccggctc
     3061 cggaaacagc tacacactgc tcaaccggga cctgatggag gaatccagcc gtaccgagtt
     3121 ccggcagtgg taccgcaagg ccgagaccca actcgacgcc gtgctcggaa cgcgccacgt
     3181 cgtcacgacg ggcggagggt atgtgggtga gacggtccgc gcggaccgcg tgcagggagg
     3241 ggagcgctcg aactccaacg gatacgtctt cggtcagcac cagtggttcc tggccgacgg
     3301 gatccaggtg cactccagcg cccgcttcga ctggcacacc gactatgcct cgcggctcag
     3361 ccccaagctc gccgtgatgg tctcgccctc ctccgtgctt cgcttccggg cctcggtggg
     3421 cacggggttc aaggcaccga ccttccagca gctgtacatg gacttcacga acccggtcgc
     3481 gggatacagc gtggtcgggt catcggatgt cgcgcgcgcc ctcgccgagt tcgagcgtct
     3541 gggccagatc gactacttcc tgacggatcc ctccgcgatc gggtcgatcc gacccgagaa
     3601 ctcggtcgcc ttcaacctgg gcaccgagtg ggacgtgacg cggacgctga acctccagat
     3661 gaacctcttc cgcaacaacg tcgaggacct gatcgagcac cagccggtcg cggccaagcc
     3721 gaacgggcag tacgtgttca cctacgtgaa cctgaaccgc atctacacgc agggcctcga
     3781 actcgaggcg gcgtgggacc cgacgtcccg atggggggtc gccgtcggat accagttctt
     3841 ggacgcgcgg gaccgtgacg tgctggaccg gatcgacgag gggtcggtgt accgccgtgt
     3901 gaacgggcgg gacgagcggc tgacgcggtc ggactacggc ggcctcttca accggtcccg
     3961 ccactcgggc tcgctgcggc tgactttccg gtcgcccggc catggcctga cctcgacgat
     4021 ccgcgcgatc taccgcagcc ggtacggcta cggcgacctg aacggcaacc tgatcctgga
     4081 cgacgaccgc gagtacgtgc ccggcttcgc gctggtgcac ctcacggtca gcaaggacct
     4141 gacgccctgg atcaccgccc aggccggcat caagaacctg ctggacacga cgtaccccac
     4201 cctggtgccg tcgctgcccg gccgcctgct cttcgcgggc gtgaacgtga cgatccgata
     4261 accatcgctt ttcatagacc atgcgcactg caccccgcct caccctgctc ctggccctcg
     4321 tcctcaccct ggggttcgcc gcctgcgact ccgacccgaa cgacggcgaa ggcctgccgc
     4381 tgaacgccac ggtcgtggag aacctgcctg ccgacccggc cgtgaacatc ggcccgatgg
     4441 gtccggtcgg caacgggcac ttcaccttct tcagcctgcg cgagaacgag atcgtgaccg
     4501 actcggcatc caccgactgg gacctcggct tccgggcgac cgagatcatc gtcaacggcg
     4561 gcgtgagcgg gcccggcgag ggacgcgcgc aggtcaggga aggcctgttc gaggaaatca
     4621 ccgaggcccc ggagagtggt tggcgaatcg acaacgagga gggccacgcc atccggacgg
     4681 ggtcgggcaa cggctggtac aactacaacc cgcaggcgat gaccgtctcg ccgatccccg
     4741 gccgcgtcct cctggtgcag acggccgacg gcaagtacgc caagctccgg atcatcagct
     4801 actacaagga cgcccccgag actcccgaca tcgacagcga gtctcgctac tacacgttcg
     4861 agtacgtctt ccagcccgac ggatcgagga tcctcgagta gcggcgcccg aacgacgagt
     4921 tgctcccggc ggggcccggt ccggaatggc acgccggatc gggcccttcc attcccccag
     4981 ccccccggtg cgatgcccag tcccatccac tacctcccga tcctgacgac cctcgtctcg
     5041 atcccgttcg ccatcgtcct cttccgccgg tggcgggcca agcccgacgc cctgtacctc
     5101 atgtggtgga tgatcggggt cctgacgtac atggccggca cggtcaccga atcgctgacc
     5161 acgctcctcg gatggcagct tcccgtgttc aaggcgtggt acatcacggg cgccctgctc
     5221 ggtggcgcac cgctcgcgca gggaacgatc catctgctct tctcccggcg cacggcaaac
     5281 atcctgtcgg ccatcctggt cacgttcgtc gcgatcgccg ccgtgccggt gctcctctcg
     5341 ccggtggacc tcaccctcgt ggagacctac cgcctctcgg gcgcggtcct ggagtggcag
     5401 tgggtccggg cgttctcgcc gttcgtgaac acgtacgcgg tcatctttct cgtgggcgga
     5461 gcggcgtggt ccgcggtgcg ctacgcgaag cagggcgaga cgatgcgggc ccggatgtgg
     5521 ggcaacatct ggatcgccgt cggtgccatt ttgcccggaa tcggcggcgg gatggcccgg
     5581 gccggcatcg tggaggggct ctatgtcggg gagctcgtcg ggctcatcct gatctggacc
     5641 ggctaccacg tgatcgccgg cgaccggagc gcctcgatcc acgagaacca gcagatggcc
     5701 gccgaggccg cccgcgccgc ctgattgcgc cgttcggggc ggttcggcta tactgcggga
     5761 ccacctgccg gccgcgccga tgaaactgct ggtcctgaac ggcccgaacc tgaacctgct
     5821 cggcacccgg gaaacggacg tctacggcac cgggacgctc gccgaactcg agcgcgcgct
     5881 gcgcgaggag ttcgccggcg aatcgctcga gttcgcgcag tccaaccacg agggcgagct
     5941 cgtcgacgcg ctgcagcggg ccgctcggga gggcttcgac ggcgtggtgt tcaacccggc
     6001 cggctattcc cacacgtccg tcgccctgcg cgacgccgtc gcggccgtcg acgttcccgt
     6061 ggtcgaggtg cacatctcca acgtgtacgc gcgcgagcgc ttccggcacg tctcccggac
     6121 cgccggcgcc gccgtcggcg tgatcgcggg gctcggcctc gccggctact cgctcgccgt
     6181 ccgctacctg ctgctccacg cctcgaaatc ctgacccggg gacgggcgcc atggccggcc
     6241 gcatccgcca gctcgcttcg gagaccgccg tatacggcgt gtcgtccatc ttcgcgcggg
     6301 cggtcaactt cctgctcttt ccgttctact cgaacgtgtt cgcgcccgac gtctacgggc
     6361 cgatcatcgt cgtgtacgcg gccttcgtct tcctgaacat cctgtaccag aac
//
LOCUS       DBNHQZ010000311         3019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1718753, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000311 DBNHQZ010000000
VERSION     DBNHQZ010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1718753"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..712)
                     /locus_tag="AC16YU_08360"
     CDS             complement(35..712)
                     /locus_tag="AC16YU_08360"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6065277.1"
                     /translation="MYQSTYQPNFRLGVFPPVIKNLLIVNGLVFLAQLVPTTNAFLTD
                     WFALWPLDTPDLPGFGSFMPWQLVTYGFLHGDLMHLLFNMFALWMFGIQIENTWGSQR
                     FAIFYFVSVIGAGLVQLAVATMGPGAYATIGASGGVFGILLAFGMMFPDQPIYVYFLL
                     PIKAKWFVIIYGALELYAGVGSYGSPIGQNAVAHFAHLGGMLFGFLLIQYWRGKLPVK
                     PDIRMHW"
     gene            872..1633
                     /locus_tag="AC16YU_08365"
     CDS             872..1633
                     /locus_tag="AC16YU_08365"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR6065278.1"
                     /translation="MILLIGDLHFVAGPSRSRTRAAVGGPADEAALVACLDHLADRLT
                     HVVLLGDVYDAYVEYRHLVPRGPVRLLGQLARLGDSGVEVTYVVGNHDPWHRTFMEQE
                     LGFRLVREAATLPMDGVRVHVAHGDLVGRSRKPLAMRIVRHPLSLALYTSLLPGDSGQ
                     ALARKMSRLAGHREDDPGLPAVLRAYAEHVLGGGSADVVAMGHSHEPAQHHGPGGSYL
                     NPGFWPRHRIVGIVEGGSATLRTWRAGALSAVDPV"
     gene            1691..>3019
                     /locus_tag="AC16YU_08370"
     CDS             1691..>3019
                     /locus_tag="AC16YU_08370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6065279.1"
                     /translation="MADWSYTEKELEDYFADPRRREPGREAKKGWFHRRFADPEKATA
                     FRALAWIAGIALLVTLSVLGYLVSLRDEMPPLTEIENPNFQFATIAYTADGVELARYA
                     RQNRAWVSYADISPHVINALIATEDHRFYDHWGIDLFRTASIPFHVLLGDPQGGSTIS
                     QQLARNLYNKRIGYEVTLARKLKEWVTAVQLERRYTKEEIIEMYLNTVEFGYNAWGLE
                     AAARTFFGKAPIDLDELESATLVGMLNGITRYNPVRNPENSRRRRNVVLGQMVKNGYL
                     DRQFLETHREDSVRTSYQSSELTASLAPYFAEHVRGWLNRWAVDSGYDVYEDGLVVYT
                     TLDSRMQKVAEDALQAQMVCLQAVVDFEWSRTSSGVWSTDACEYTKETGYEPFGHFWS
                     SQPGLLDQLIRESDRYRSMRRSGMAPDEATSALRANAPFVDSLKAAKTRLE"
BASE COUNT          552 a          982 c          993 g          492 t
ORIGIN      
        1 tcggctgcga cgtattcgga gggtcggagc agcatcacca gtgcatgcgt atgtcaggct
       61 tgacgggcag cttcccccgc cagtactgga tcaggagaaa gccgaaaagc atgccaccga
      121 gatgcgcgaa atgggcgacg gcgttctgcc cgatcgggct tccataagat cctacaccgg
      181 catagagctc gagcgctcca taaatgatca cgaaccactt cgccttgatc ggaaggagga
      241 agtaaacgta gatgggttga tcgggaaaca tcatgccgaa ggccagcaga atcccgaaga
      301 ctccacccga cgccccgatg gtcgcgtaag ctcccggccc catcgttgcc accgcaagct
      361 gcaccaggcc ggcgccgatc acgctcacga agtagaagat cgcaaagcgc tgcgagcccc
      421 acgtgttctc gatctggatg ccgaacatcc agagggcgaa catattgaag agcaggtgca
      481 tgaggtcccc gtgcaggaac ccgtacgtca cgagttgcca gggcatgaag ctgccgaagc
      541 cgggcaggtc cggtgtgtcg agcggccaca gcgcaaacca gtcggtcagg aaggcgttcg
      601 tcgtcggtac cagctgcgcc aggaatacca ggccgttcac gatcaacagg ttcttgatca
      661 ccggggggaa cacccctaac ctgaagttcg gctgatacgt cgactggtac atggacatcc
      721 gtcgggtggg cggtctggcg gcgttcggga ccgtagcaat tctaaggtac tcgaaacgtt
      781 cctccgtccg gcacggtatc ctgaggccgg tttctgcgcg gttcgccgcg aaggaacggt
      841 cttcccttgc acgcaccctc cctgtcggac cgtgattctg ctgatcggtg acctgcactt
      901 cgtggccggc cccagccgta gccggacccg ggccgcggtc ggtggcccag cggacgaggc
      961 ggcgctcgtc gcatgcctgg atcacctggc ggaccgcctg acccacgtgg tgctcctcgg
     1021 cgacgtgtac gacgcctatg tcgagtaccg gcacctggtg ccgcgcggcc ccgtgcggct
     1081 cctcgggcag ctcgcccgcc tgggtgattc cggggtggaa gtcacgtacg tggtcggcaa
     1141 ccacgatccg tggcaccgga cattcatgga acaggagctg ggattccgcc tcgtcaggga
     1201 ggccgcgacc ctcccgatgg acggcgtccg cgtgcacgtg gcgcacggcg acctggtcgg
     1261 acggtctcgg aagccccttg cgatgcgaat cgtccggcac ccgctctcgc tcgcgctcta
     1321 cacgagcctg cttcccggcg attccgggca agccctcgcc cggaagatga gccgcctggc
     1381 gggccacagg gaggacgatc cgggcctccc tgccgttctc cgcgcgtacg ccgagcacgt
     1441 gctcgggggc ggctcggcgg acgtcgtggc catggggcac agccacgagc cggcgcagca
     1501 ccatggtccc ggcggctcgt acttgaatcc gggcttctgg ccgcgacacc ggatcgtcgg
     1561 gatcgtggag ggcggctcgg cgacgctgcg gacctggagg gcgggcgcac tgagcgccgt
     1621 cgaccccgtg tgaagatccg agaaaccccg gcgcccgctc gatcgttgcc tgactcgaat
     1681 cgggggaatc atggcagact ggtcctacac cgagaaagag ctcgaggatt acttcgcgga
     1741 ccccaggcgt cgcgagccgg gcagggaggc gaagaagggg tggttccacc ggcgattcgc
     1801 cgatcccgag aaggcgacgg cattccgggc gctcgcgtgg atcgcgggga tcgcgctcct
     1861 ggtcacgctg tcagtcctgg gttacctcgt ctcgctccgt gacgagatgc cgccgctgac
     1921 ggagatcgag aacccgaact tccagttcgc gacgatcgca tacacggccg acggcgtcga
     1981 gctcgcgcgg tacgcccgtc agaaccgggc ctgggtttcg tacgccgaca tctcgccgca
     2041 tgtcatcaat gcgctgatcg ccaccgagga tcaccgcttc tacgaccact ggggcatcga
     2101 cctcttccgg acggcgtcca tcccgttcca cgtgctgctg ggcgatcccc agggcggctc
     2161 gaccatctcg cagcagctgg cccgcaacct ctacaacaag cgcatcggtt acgaggtcac
     2221 cctcgcgcgg aaactgaagg agtgggtgac ggcagtccag ctcgagcgcc gctacacgaa
     2281 ggaagaaatc atcgagatgt acctgaatac ggtagagttc gggtacaatg cctggggtct
     2341 cgaagccgcc gcccgcacgt tcttcggcaa ggcgcccatc gacctggacg aactcgagag
     2401 cgcgacgctc gtcggcatgc tcaacgggat cacccggtac aacccggtcc gcaatcccga
     2461 gaactcccgg cgccggcgga acgtggtcct tggacagatg gtcaagaacg ggtacctcga
     2521 ccggcaattc ctcgagacgc acagggagga ttcggtccgc acgtcgtacc agtcgtccga
     2581 gttgacggcc agcctcgccc cctacttcgc cgagcatgtc cgcggatggc tgaaccggtg
     2641 ggcggtcgac agcggatacg acgtgtacga ggacgggctg gtcgtctaca cgacgctcga
     2701 ctcgcgcatg cagaaggtcg cggaggacgc tctccaggcc cagatggtgt gtctgcaggc
     2761 ggtcgtggac ttcgagtgga gcaggacgtc gtccggggtc tggagcacgg acgcctgcga
     2821 gtacactaag gaaacggggt acgagccctt cgggcatttc tggagctctc agccgggtct
     2881 cctcgaccag ttgatccgcg aatccgaccg gtaccgctcc atgcgccgga gcggcatggc
     2941 gcccgacgag gcgacgtccg cgctccgggc gaacgcaccg ttcgtggatt cgctgaaggc
     3001 cgcgaagacc cgcctcgag
//
LOCUS       DBNHQZ010000312         6532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3345219, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000312 DBNHQZ010000000
VERSION     DBNHQZ010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3345219"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="AC16YU_08375"
     CDS             <1..760
                     /locus_tag="AC16YU_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR6065280.1"
                     /translation="RFRDDAEEAVDLLEVDNRAVLARQVERLGRLRATRDAEAVRRAL
                     DALSRAARSGEGNLLELSVVAARARATLGEISGALERAFGRYEADIPTVSGVYASEMQ
                     NDPAFERARDLARRFAERHGRRPRILVAKLGQDGHDRGARVVATAFADLGFDVDVGPL
                     FQMPEEAARQAVENDVHFVGISSLAGGHKTLVPALVDALARLGRPDILVVAGGVIPPA
                     DYEMLYAAGVAAVFGPGTVIPEAAGRLLGLLVDA"
     gene            757..1860
                     /gene="meaB"
                     /locus_tag="AC16YU_08380"
     CDS             757..1860
                     /gene="meaB"
                     /locus_tag="AC16YU_08380"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR6065281.1"
                     /translation="MNPPQARSTGSTPEDDGLSVRPGTTGAPSVNPGLRVGAPDLPDA
                     ADLFRGMREGDRVALARAITLVESVLERDRARSLELLDLCQPHAGGAIRVGFSGAPGV
                     GKSTLIDALGVDLVEAGRRVAVLAVDPSSERSGGSILGDKTRMGRLSALPGAYVRPTP
                     SGGSLGGVARFTRPAITLVEAAGFDVVIVETVGVGQSETAVRHMVDTLVLLSMSGAGD
                     ELQGIKRGIVEAADIVAVTKADGDGRDAALAARGVLRQALHLLPPDDTDWRVPVLAVS
                     ALDRSSATGLWEAVEAHHGHLVRTGTLEARRKIQGVRWLRDELTDRLLERFRAGAGLA
                     DALARAEEAVASGELHPYRAADDLVRRFLPEGT"
     gene            complement(1857..3350)
                     /gene="gltX"
                     /locus_tag="AC16YU_08385"
     CDS             complement(1857..3350)
                     /gene="gltX"
                     /locus_tag="AC16YU_08385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR6065282.1"
                     /translation="MPAQVSLPPRVRFAPSPTGYLHIGGLRTALYNYLVARRHGGTFI
                     LRIEDTDRSRFVPDAEEDILSALRWAGLEFDEGPGRPGPYGPYRQSERRALYAEHAAR
                     LVEEGHAYLAFDTPAEIESMRERMATDAEPNPRYDASTRLSMRNSLSLAEGETGQLLE
                     QSVPHVVRLRVDPGAEVRFVDLVRGEVSFATDGIDDQVLVKSDGMPTYHLANVVDDHH
                     MRITHVIRGEEWLPSTPKHILLYRAFGWEPPAMAHLPLILSPNGGKLSKRNADQLGIP
                     VFASRYREAGYEPSALVNYLAFLGWNPGDEREVLTLDELVEAFSIDRVGSAGVQFDAA
                     KLAWYNQQHLRRVPPEEIARRAAPALEAAGIVSGAGYPERVVEVLGERLSFPADLASE
                     WRFFFSDPDAYDQDGVRKRWKEDSAALVSAYAESLPPPDDFTPEALESALRTLAETSG
                     VGAGRIIHPVRLAVSGVTAGPGLFEMLSVLGREACVRRLTRAAERLG"
     gene            3493..5550
                     /locus_tag="AC16YU_08390"
     CDS             3493..5550
                     /locus_tag="AC16YU_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6065283.1"
                     /translation="MKIPVPYAVLLVIAAVLLSACSGSRSTTGQQASASDVLAEIDGQ
                     PVTVAEFESQYLRAISDTAQAIAATRDEYEDFLNRYVDFRLKVLEAERLGYGNLSDLN
                     AELETYRGQLARPYLLEREVTEPLVRQLYERRAEMVDVSHILIRVAENAPPEDTLRAY
                     EKLSAVADSIAHGADFGEMALRHSEDPSARAAQGSIGYQGRLGFFTAGRMVAPFEDRM
                     YTTPVGGISPVFRTQFGYHVLEVHGRRPTVPDRRISHIMIQPRGPAEADSADAVARLD
                     AALAALGSGQSFAAVAAEYSNDVQTAQRGGDLDYIAYDGWLPVEMRDVAFAADSVGAV
                     LGPVRTRFGWHLMQVTDVRRRPSFDESYDALQTEVARMPRSRQAEKAFARSLRQRYDA
                     RVDSSLFGRLRGTFEPDSLLGSLRAGTVDSTLLAMPFATVGDSTWPLAQFAGFLGTGG
                     ARLTAETPGGRLREAIDAWLDDRAIAYEITALQERDADFARTMREFRDGLMLFRLMED
                     SVWSAAATDSLALVAYHAAHVDSFRFGERHRILSIASPSDSLLQAIAAELQGGSSYAD
                     VLSRFASDPASEVRFDTTYVEGSTDSVFDRAIALEPGQTTGVVGFGSGRIILVNDGIE
                     PPRTKTFEEARAEVVGRYQAIIEERLMERLRTEHGVRLHPERLDRVFAGARARTALGV
                     PSS"
     gene            5547..6416
                     /locus_tag="AC16YU_08395"
     CDS             5547..6416
                     /locus_tag="AC16YU_08395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6065284.1"
                     /translation="MSASPSARPAGRRALVLAAALLLAGCSAEPDADEFVARVGDRLL
                     TKEDLGQAIQVDRLGLDSVETARRYIDQWITSELLFAEARRRGLRADPGVKRRLEESE
                     RSILVSALVDQLYRNPTAEASLEDVITYFESHRDQLGLLEPFVRIRYLLLANPDSAAV
                     AARALASVDTSDAEGWLAVADRFALDPEAVRDLAGTYAAETRLMAHLPRVRQAIGALA
                     PGRTSPVIEDGGQYHVVQLVDRVPAGTVPRIEWIEPALRQRLQIEARKQLYARLVQDL
                     RNEAVARDLIETH"
BASE COUNT          924 a         2344 c         2268 g          996 t
ORIGIN      
        1 ccgcttccgg gacgacgcgg aggaggccgt ggacctgctg gaggtggaca accgggcggt
       61 gctcgcgcgc caagtcgagc ggctcgggcg gctgcgggcc acgcgggacg cggaggccgt
      121 gcgtcgggca ctcgacgccc tctcgcgcgc ggcccggtcc ggcgagggca acctgctcga
      181 gctctccgtc gtggccgcac gcgcccgggc gaccctcggc gagatttccg gagcgctcga
      241 gcgtgcgttc ggacgctacg aggcggatat tcccacggtc tccggcgtct acgcctccga
      301 aatgcagaac gatccggcct tcgagagggc ccgcgacctg gcgcgccgct tcgcggagcg
      361 tcacgggcgg cggccgcgca tcctggtcgc caagctgggc caggacgggc atgaccgagg
      421 cgcccgcgtg gtcgcgacgg ccttcgccga cctgggattc gacgtggacg tgggccctct
      481 cttccagatg ccggaagagg ccgcccggca ggccgtcgag aacgatgtcc acttcgtggg
      541 catttccagc ctggccggcg ggcacaagac cctggtcccg gccctcgtcg acgccctcgc
      601 acggctgggc cgtcccgaca tcctcgtggt cgccgggggc gtcattccgc cggccgatta
      661 cgagatgctg tacgccgccg gcgtcgccgc cgtcttcggc ccgggcaccg tgatcccgga
      721 ggccgccggc cgactgcttg gcctcctcgt ggacgcctga accctccgca agcccgttcc
      781 accgggtcca cccccgaaga cgacggcctc tccgtccggc ccggcacgac cggggcgccc
      841 agcgtcaatc ccggcctccg ggtaggagcc ccggacctcc cggacgccgc cgacctcttc
      901 cggggcatgc gcgagggcga tcgtgtggcc ctggcccgcg ccatcacgct cgtcgagagc
      961 gtgctcgagc gggaccgtgc ccgatccctc gagctgctgg acctgtgtca gccccacgcc
     1021 gggggcgcga tccgggtcgg cttctccggc gctccgggcg tcgggaagag cacgctcatc
     1081 gacgccctcg gagtcgacct ggtcgaagcg ggccggcgcg tcgcggtgct cgccgtcgat
     1141 ccgtcgtcgg agcgctcagg cggcagcatc ctcggcgaca agacccggat gggacggctc
     1201 tcggcgcttc cgggcgccta cgtgcggccg actccgtccg gtggttcgct cggaggcgtc
     1261 gcccgcttta cccgccccgc catcacgctc gtggaagcgg ccggctttga cgtcgtgatc
     1321 gtcgagacgg tgggcgtcgg ccagtccgag acggccgtgc gccacatggt cgataccctg
     1381 gtactgctga gcatgtccgg cgccggcgac gaactgcagg gcatcaagcg gggcatcgtc
     1441 gaggccgccg acatcgtcgc cgtgaccaag gccgacggcg acggccgcga cgcggcgctt
     1501 gccgcgcggg gcgtgctccg ccaggccctg cacctgcttc cccctgacga tacggactgg
     1561 cgggttccgg tcctggccgt ctcggcgctc gaccgctcct cggccacggg cctctgggag
     1621 gccgtagaag cccaccacgg ccacctggtc cggaccggca ccctcgaggc gcggcgaaag
     1681 atccaggggg tgcggtggct gcgcgatgag ctcacggaca ggctcctgga gcggttccgg
     1741 gcgggagcgg gccttgcgga tgcgctcgcc cgggcggaag aggccgtggc gtcaggagaa
     1801 ctgcatccgt accgggccgc ggacgacctg gttcgtcgct ttctgcccga ggggacctaa
     1861 ccgagccgtt cggcggcccg tgtcaggcgc cgcacgcacg cctcgcgtcc caggaccgac
     1921 agcatctcga agagcccggg acctgccgtg acgccgctga cggccagtcg caccgggtga
     1981 atgatcctcc cggcccccac accgctcgtc tcggcgaggg tgcgcagggc ggactccagg
     2041 gcctccgggg tgaagtcgtc cgggggcggg agcgattccg cgtaggcgga caccagtgcg
     2101 gcggagtcct ccttccaccg cttgcggacc ccgtcctggt cgtacgcatc cgggtcggag
     2161 aagaagaacc gccactccga cgccaggtcg gccggaaacg agaggcgctc ccccagaacc
     2221 tcgactactc gctccgggta tccggcgccg gacacgatcc cggccgcctc gagcgccggc
     2281 gcggcccggc gggcgatctc ctccggcggc acgcgccgga ggtgctgctg gttgtaccag
     2341 gcgagcttcg cggcgtcgaa ctgcacgccg gccgagccga cgcgatcgat cgagaacgcc
     2401 tcgacgagct cgtcgagcgt gagcacctcg cgctcgtccc cggggttcca gcccaggaag
     2461 gcgaggtagt tcaccagcgc cgaaggttcg tacccggcct cccggtaccg ggaggcgaag
     2521 accggaatgc ccaactggtc cgcgttgcgt ttcgacagct tgccgccgtt cgggctcagg
     2581 atcagcggca ggtgggccat tgcgggcggc tcccagccga aggcacggta cagcaggatg
     2641 tgcttgggcg tcgacgggag ccactcctct ccacggatca cgtgcgtgat gcgcatgtgg
     2701 tggtcatcga ccacgttcgc caggtggtac gtgggcatgc cgtccgattt caccagcacc
     2761 tggtcgtcga tcccgtccgt ggcgaaggac acctcgccgc gcaccaggtc gacgaatcgc
     2821 acctcggcgc ccgggtccac ccggagccgc acgacgtgcg gcacggactg ctccagcagt
     2881 tgcccggtct cgccctctgc cagtgacagg ctgttccgca tcgagagccg ggtcgaggcg
     2941 tcgtagcgtg gattcggctc ggcatcggtg gccatgcgct cccgcatgga ctcgatctct
     3001 gcgggcgtgt cgaacgcgag gtaggcgtgg ccctcctcga ccagccgggc ggcatgctcc
     3061 gcgtacagcg cccgccgctc ggactgtcgg tacgggccgt acggaccggg ccgccccggt
     3121 ccttcgtcga actcgagtcc ggcccagcgg agcgccgaaa ggatgtcctc ctcggcatcc
     3181 ggcacgaacc tggaccggtc cgtgtcctcg atgcgcagga tgaacgtccc tccgtgccga
     3241 cgtgcgacga ggtagttgta cagtgccgtg cggagtcccc cgatgtgcag gtagccggtc
     3301 ggcgaaggcg cgaaacggac tcggggtggc agggatacct gggcgggcat ggagcacgga
     3361 cggattgcga aacgcctcaa actacaagtg cccgaaatag gaaccatccg cgacgcctcc
     3421 gcgtgtgtgg ggcgcttttc cgagatcggc acttccggtg ccggccgtca tgaacacaca
     3481 aggcccttga ccgtgaagat cccagtccct tacgccgtcc ttctcgttat cgccgcagtc
     3541 ctcctgtccg cctgctcggg ctcccgcagt acgaccggac agcaggcgtc cgcctccgac
     3601 gtgctcgccg aaatcgacgg gcagcccgtc acggtcgcgg agttcgaaag ccagtacctg
     3661 cgcgccatct cggacacggc ccaggccatc gcggccacgc gcgacgaata cgaggacttc
     3721 ctgaaccggt acgtcgactt ccgcctcaag gtgctcgagg ccgagcggct cgggtacgga
     3781 aacctctccg acctgaatgc cgagctcgag acgtaccggg ggcagctcgc acgtccgtac
     3841 ctgctggaac gcgaggtaac cgagccgctg gtccgccagc tgtacgagcg cagggccgag
     3901 atggtcgacg tcagccacat cctgatccgc gtcgcggaga acgcgccccc ggaggacacc
     3961 ctgcgcgcat acgaaaagct ctccgcagtg gccgactcga tcgcccatgg tgccgacttc
     4021 ggcgagatgg cgctgcgcca ttcggaggac ccctcggccc gcgcggccca gggatccatc
     4081 ggttaccagg ggcgcctggg gttcttcacg gccgggcgca tggtcgcgcc gttcgaggac
     4141 aggatgtaca cgacccccgt cggcgggatt tcccccgtct tcaggaccca gttcggatac
     4201 cacgtcctcg aggttcacgg tcgccggccg accgttccgg accgccggat ctcgcacatc
     4261 atgatccagc cccgtgggcc ggccgaggcg gattcggccg acgcggtggc ccggctcgat
     4321 gcggcactcg cggcgctcgg gtcggggcag tcgttcgcgg cggtcgcggc cgaatattcg
     4381 aacgacgtgc agaccgcgca gaggggcggc gacctggact acatcgccta tgacggctgg
     4441 cttcccgtcg agatgcgcga cgtggccttc gcggcggaca gtgtcggcgc ggtgctcggc
     4501 cccgtgcgca cccgcttcgg gtggcacctc atgcaggtga ccgacgtgcg caggaggccc
     4561 tcgttcgacg agtcctatga cgcgctccag accgaggtgg cgcggatgcc ccgatcccgg
     4621 caggcggaga aggccttcgc gcgctcgctc aggcagcgct acgacgcccg ggtggattcc
     4681 tcgctcttcg gccggctccg cgggacgttc gagcccgaca gcctgctggg gtcgctcagg
     4741 gcaggtacgg tcgactccac gctcctggcc atgccgttcg ccaccgtcgg cgattccacc
     4801 tggcccctcg cgcagttcgc cggattcctg ggcacgggcg gagcccgcct cacggccgag
     4861 acgccaggcg gccgactccg ggaggccatc gatgcgtggc tcgacgaccg ggcgatcgcc
     4921 tacgagatca cggcgctcca ggagcgcgac gcggacttcg cccgcacgat gcgcgagttc
     4981 cgcgacggtc tcatgctgtt ccggctcatg gaggattccg tatggagcgc ggccgcgacg
     5041 gacagcctgg cgctggtggc ctaccatgcg gcgcacgtgg attccttccg cttcggggag
     5101 cgtcaccgca tcctgagcat cgcatcccct tcggattcgc tcctccaggc catcgccgcg
     5161 gagctccagg gcgggtcctc ctatgccgac gtcctttccc gcttcgcctc cgatccggcg
     5221 tcggaggtgc gcttcgacac gacgtacgtc gagggctcca cggattccgt cttcgaccgg
     5281 gccatcgcgc tcgagccggg ccagacgacc ggggtggtgg gcttcgggtc gggtcgcatc
     5341 atcctcgtca acgacggcat cgagccgccg cgcacgaaga ccttcgagga ggccagggcc
     5401 gaggtcgtag gccgctacca ggcgatcatc gaggagcgcc tgatggagcg actgcgcacg
     5461 gagcacgggg tgcggctgca tccggagcga ctggaccgcg tcttcgccgg tgcccgcgca
     5521 cgaacggcac tgggggtccc ctcctcatga gcgcgagtcc gagtgcgcgg cccgcgggcc
     5581 gacgggccct ggtcctggcg gcggccctgc tgctggcagg ctgctccgcg gagcccgatg
     5641 ccgacgagtt cgtggcccgc gtcggggatc ggctgctgac gaaggaagac ctgggccagg
     5701 ccatccaggt ggaccgcctc gggctcgatt ccgtcgaaac cgcccgccga tacatcgacc
     5761 agtggattac ttccgagctg ctcttcgccg aggcgaggcg gcgcggcctg cgtgccgatc
     5821 ccggggtcaa gcggcggctc gaggagagtg agcgttcgat cctcgtctcg gcgctcgtgg
     5881 accagctgta ccgcaatccg acggcggagg cgtctctgga ggacgtgatc acgtacttcg
     5941 aatcccatcg cgaccagctg gggctcctgg agccgttcgt gcgtatccgc tacctgctgc
     6001 tcgcgaaccc tgattccgcg gccgtcgccg cgcgtgcgct cgcgtcggtg gacacttccg
     6061 acgccgaagg ctggctggcc gtggcggatc ggttcgcact cgatcccgag gcggtccggg
     6121 acctggccgg tacctacgcg gccgagaccc gcctcatggc ccacctgccc cgcgtgcgcc
     6181 aggccatcgg ggccctcgcc ccgggacgga cctcgcccgt catagaggac ggcggacagt
     6241 accacgtcgt gcagctggtg gaccgtgtcc cggccggcac ggtgccgcgg atcgagtgga
     6301 tcgagcccgc gctcaggcag cggctccaga tcgaggccag gaaacagttg tatgcccgtc
     6361 ttgttcaaga tttgcgcaac gaggccgtcg cccgcgatct catcgagacg cactgacccg
     6421 cgccccgttc aggcaggccc acacacgatt ctgtgcgggc ccttcgtttg tggggcatgc
     6481 ccgcccattc gtacctgcca cccacctctt ccgcccgcgt gacccgaccc ct
//
LOCUS       DBNHQZ010000313         4176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_364165, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000313 DBNHQZ010000000
VERSION     DBNHQZ010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_364165"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /gene="purN"
                     /locus_tag="AC16YU_08400"
     CDS             <1..224
                     /gene="purN"
                     /locus_tag="AC16YU_08400"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR6065285.1"
                     /translation="SGATVHLVDDEYDTGPIVLQNEVPVLEDDTPESLAARVLAVEHR
                     LYPEALDLFARDRIRVEGQRVLVLPDPDS"
     gene            252..1844
                     /gene="purH"
                     /locus_tag="AC16YU_08405"
     CDS             252..1844
                     /gene="purH"
                     /locus_tag="AC16YU_08405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6065286.1"
                     /translation="MIQVKDLPDPDDRYPVRRALLSVFDKTGLVDLARALHGLGVELV
                     STGGSARTLAEAGLPVTAVSDLTGYPEILDGRVKTLHPAVHGGLLARRTDPGDLEQLR
                     DLGIGPIDLVVVNLYPFGQATSGADTPDAVAVENIDIGGPTMLRSAAKNFFFVGVVTD
                     PADYAAVAEELESNGGTLGLATRRELARKVFAHTAAYDAAVTAFFDRTRTAPSPAPVV
                     GFAAPLALPLRYGENPHQQAGLYGDPGRFFEKLHGKELSFNNLLDLSAAIHLIDEFQG
                     ADPTVAILKHTNPCGVATAPSLEEAYAAAFATDRQSPFGGIVVVNRPLDRAAAEVIDA
                     VFTELVIAPGFEDGVLEFLEQKKNRRLIRLLAPARADRGPDIRSVVGGLLVQDRDPAL
                     PDARALRDRLRVVTRRSPTEAEWLDLDFAWRVAKHVKSNAIVYARGRATLGIGAGQMS
                     RIDASEIAVRKGAKSDLDFSSSVVASDAFFPFADGLVEAARAGAVAAIQPGGSVRDEE
                     VIAAADENDMAMVFTGFRHFRH"
     gene            1872..2684
                     /gene="mreC"
                     /locus_tag="AC16YU_08410"
     CDS             1872..2684
                     /gene="mreC"
                     /locus_tag="AC16YU_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6065287.1"
                     /translation="MLRLWERFGDWILFAALIIASVVIILSWNEPMIKGFRARSLSLT
                     GTVESTFAVAGGYLRALEENESLRAENIRLSGEVARLREASLENERLRDMLAYADSLP
                     PERLPARIVYKDILRERNTLTIDVGRRHGVEVDMAVIDPRGIVGKVVLVGERFSQVMS
                     YLNTEFFVPAKIQPHQSDGIVRWSGERRDLLEMDHVMKTDPVEPGQPVVTSGYSSIFR
                     PGLPIGVVERVEELSGTPSWRVFVRPLAQLGDAEHVFVLLERPHPERIPPPS"
     gene            2695..>4176
                     /gene="dacB"
                     /locus_tag="AC16YU_08415"
     CDS             2695..>4176
                     /gene="dacB"
                     /locus_tag="AC16YU_08415"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR6065288.1"
                     /translation="MTRRRNPGRAPWRLAVLLPLALVAASPAHAQPATGSEAELRRAL
                     DRILSEAGLENALWGVAVHDLTRDRSVYAHNDRVNHVPASNTKLYTTAAALHLLGPEF
                     RYRTDLFAAGPVVDGVLRGPLVVRGSGDPVLGPRFSGDATLVFRQWADSLRVHGIRVV
                     DGDIIGDDDIFDDQALGYGWSWDDEPFWYSAEVSGLSYHDNTVDFAAVGTSAGAPARL
                     SWEPASTSYMQVLNATVTTAAGRVVESWERGRSDNRLRVASSVPEGEADNESISVSNP
                     TVYFVHVLRETLLGAGIAVRGAPRDVDELPIRPQYDAPGLVRVAGHESEPLSDVVYVL
                     NKRSQNLYAERVLKSIGAYAGERAAIGSSAARSWRAARPMFAEAGIDTLSLQLVDGSG
                     LSRQNLVSARATLALLRFMWNHPSEAVRDAYLFSLPVGGIDGTLASRYPDGRARGNVR
                     AKTGTVSNASALSGYVRTDGGSSFAFSIMANHFTVPASRIREIQ"
BASE COUNT          610 a         1534 c         1389 g          643 t
ORIGIN      
        1 tgtccggcgc caccgtgcac ctggtcgacg acgagtacga caccggaccc atcgtgctgc
       61 agaacgaggt ccccgtgctc gaggacgaca cgccggagag cctcgccgcc cgggtactcg
      121 ccgtggagca ccgcctgtat cccgaggcgc tggatctttt cgcgcgcgac cggatccgcg
      181 tcgaaggcca gcgcgtgctc gtccttcccg atcccgattc ctgatccaga ccaccaagcc
      241 agccgatttc catgatccag gtcaaggacc tcccggatcc cgacgaccgg tatccggtcc
      301 gacgggccct cctttccgtc ttcgacaaga cgggactcgt cgacctggcc cgcgcgctgc
      361 acgggctggg cgtggagctc gtctcgaccg ggggctcggc gcggaccctc gcggaggcgg
      421 gcctgcccgt caccgcggta tcggacctga cgggataccc ggaaatcctc gacgggcggg
      481 tcaagaccct gcaccccgcc gtgcacgggg gcctgctcgc ccggcggacc gaccccggcg
      541 acctcgagca gctgcgcgac ctggggatcg gcccgatcga cctcgtcgtc gtcaacctgt
      601 accccttcgg ccaggccacc tccggcgcgg acacccccga tgccgtcgcc gtcgagaaca
      661 tcgacatagg cggcccgacg atgctccgct ccgcggccaa gaacttcttc ttcgtggggg
      721 tcgtgacgga ccccgcggac tacgcggccg tcgcggagga gctcgaatcg aacggcggca
      781 cgctcggcct cgcgacccgg cgggagctcg cccggaaggt gttcgcgcac acggccgcat
      841 acgacgcggc cgtcacggcc ttcttcgacc ggacccggac cgccccttcc ccggcgcccg
      901 tggtcgggtt cgcggccccg ctcgcactcc ccctgcgata tggcgagaat ccgcaccagc
      961 aggccggcct gtacggggat ccggggcgct ttttcgagaa actgcacggc aaggagctct
     1021 ccttcaacaa tctgctggat ctctccgccg cgattcacct catcgacgag ttccagggcg
     1081 ccgatccgac cgtcgccatc ctcaagcaca cgaatccgtg cggcgtggcc accgccccct
     1141 cgctcgaaga ggcgtacgcg gcagccttcg cgacggaccg ccagtcaccc ttcggcggga
     1201 tcgtcgtggt gaaccgcccg ctggaccgcg cggccgccga ggtgatcgac gccgtgttca
     1261 ccgaactcgt gatcgccccg gggttcgagg acggggtgct ggaattcctg gagcagaaga
     1321 agaaccgccg gctgatccgg ctcctcgccc cggcgcgggc cgaccgcggg ccggacatcc
     1381 gctccgtggt cggtggcctc ctggtccagg atcgggatcc cgcgctcccg gacgcccgtg
     1441 ccctgcgcga tcgcctgcgc gtcgtgacgc ggcgctcgcc gacggaggcc gagtggctgg
     1501 acctggactt cgcctggcgc gtggccaagc acgtcaagag caacgcgatc gtgtatgccc
     1561 gcggccgggc cacgctcggg atcggagccg gccagatgag ccggatcgac gcgtccgaga
     1621 tcgccgtgcg caagggcgcc aagtccgacc tcgatttctc gagctccgtc gtggcctcgg
     1681 acgcgttctt tccgttcgcg gacgggctgg tcgaggcggc ccgggcgggc gccgtcgccg
     1741 cgatccagcc ggggggctcg gtccgcgacg aggaggtcat cgccgccgca gacgaaaacg
     1801 acatggccat ggtgttcacg ggtttccgac atttccgaca ttagaccccg ccacaaccca
     1861 ccggacaaag catgctccgc ctctgggaac gattcggcga ctggatcctg ttcgcggccc
     1921 tgatcatcgc atcggtcgtg atcatcctgt cgtggaacga gcccatgatc aagggcttcc
     1981 gggcgcgctc gctgagcctg accgggacgg tcgagagcac gttcgcggtc gccggcggct
     2041 acctgcgggc cctggaggag aacgaatcgc tccgggcgga gaacatccgc ctctcgggcg
     2101 aggtggcccg gctccgggaa gcgtcgctcg aaaacgagcg cctgcgggac atgcttgcct
     2161 acgccgattc cctcccgccg gagcggctcc cggcacgcat cgtctacaag gacatacttc
     2221 gcgagcgaaa cacgctcacg atcgacgtgg gccggcggca cggcgtcgag gtggacatgg
     2281 cggtcatcga tccacggggc atcgttggca aggtggtcct cgtcggagag cgcttctcgc
     2341 aggtgatgtc ctacctgaac accgagttct tcgtgcccgc gaagatccag ccacaccagt
     2401 cggacggcat cgtccggtgg agcggcgagc gcagggacct gctcgaaatg gatcacgtca
     2461 tgaagaccga tccggtggag cccggccagc cggtcgtcac gtcgggctac tcgtccatct
     2521 tccgtcccgg cctgcccatc ggggtggtgg aacgggtcga ggagctttcg gggacgccgt
     2581 cgtggcgcgt cttcgtgcgg ccgctcgcgc agctcggcga cgcggagcac gtcttcgtcc
     2641 tcctggagcg cccccatccg gagcgcatcc ctcccccctc ctgatcctcc cgtcatgacc
     2701 cgacgccgga atccgggccg cgccccgtgg cgcctggccg tcctgctgcc gctcgccctc
     2761 gtcgccgcct cgcccgccca cgcccagccg gccacgggga gcgaagccga gctgcgtcgc
     2821 gcgctcgacc ggatcctgtc cgaggccggg ctcgaaaacg ccctctgggg cgttgccgtg
     2881 cacgacctga cccgcgaccg gtccgtatac gcgcacaacg accgtgtgaa ccacgtcccg
     2941 gcgtcgaaca cgaagttgta cacgacggcg gccgccctgc acctgctcgg ccccgagttc
     3001 cggtaccgca ccgacctctt cgcggccggc ccggtggtcg acggggtcct ccgcggcccc
     3061 ctcgtagtgc gcggcagcgg cgaccccgtg ctcgggccgc gattcagcgg ggacgcgacc
     3121 ctggtcttcc gccagtgggc cgacagtctc cgggtccacg gcatccgcgt cgtcgacggg
     3181 gacatcatcg gggatgacga catcttcgac gaccaggcgc tcggctacgg ctggagttgg
     3241 gatgacgagc cgttctggta ctcggccgag gtgagcgggc tgtcctacca cgacaacacc
     3301 gtggacttcg cggccgtcgg gacgagcgcc ggtgctccgg cccgcctgtc gtgggagcct
     3361 gcctccactt catacatgca ggtcctgaac gcgaccgtga ccacggcggc cggccgcgtg
     3421 gtcgaatcgt gggagcgcgg ccgctccgac aaccgcctcc gggtcgcgtc gagcgtgccc
     3481 gagggagagg ccgacaacga gtcgatctcg gtgtcgaacc cgaccgtgta tttcgtgcac
     3541 gtgctgcgcg agacgctcct cggggccggc atcgccgtgc gcggcgcgcc gcgtgacgtg
     3601 gacgagctcc cgatccgccc ccaatacgac gcccccggcc tcgttcgcgt ggccgggcac
     3661 gagtccgagc cgctttcgga cgtcgtgtac gtgctcaaca agcgaagcca gaacctctat
     3721 gccgagcggg tgctcaagtc gatcggggcg tacgcgggcg agcgcgccgc catcggctcg
     3781 tcggccgccc gctcgtggag agcggcccgg cccatgttcg ccgaggcggg tatcgatacc
     3841 ctgtccctgc agctcgtcga cggatcgggc ctgtccaggc agaacctcgt gtcggcccgc
     3901 gcgacgctgg cgctcctccg cttcatgtgg aaccatccgt ccgaagcggt ccgcgacgcg
     3961 tacctcttct ccctccccgt cggcggcatc gacggcacgc tagcgtcccg ctatccggac
     4021 ggacgggccc gcgggaacgt gcgcgccaag accggtacgg tatcgaacgc cagcgcgctg
     4081 tccggctatg tccggacgga cggaggctcc agtttcgcct tctcgatcat ggccaaccac
     4141 ttcacggtgc cggcctcccg gatccgcgag atccag
//
LOCUS       DBNHQZ010000314         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2124382, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000314 DBNHQZ010000000
VERSION     DBNHQZ010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2124382"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16YU_08420"
     CDS             <1..326
                     /locus_tag="AC16YU_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065289.1"
                     /translation="SDGFREAVASDGFREALTSDGFREALTSDGFREALTSDGFREAL
                     ASDGFREALVSDGFREALASDGFREALASDGFRELLGSDAFRTLALRESLSQSFLREA
                     HRLEQ"
     gene            439..>2466
                     /locus_tag="AC16YU_08425"
     CDS             439..>2466
                     /locus_tag="AC16YU_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065290.1"
                     /translation="MRLLRFATGTRRAAGLRSHPPRPVLGRVAAFVVCVGTAAPGALA
                     GQFRTLETGNLQLVYSSPLQSHLVPQVARSFENSLQFYHDLFQYTPRGKVGLLMHDLW
                     HYGNAGARATPENHITVGIAPNQHVYESSPAPERMVSALNHEMAHIVTIDKAASTDRF
                     FRSIFFGKVRANAEVPLSMLYGYLTAPRWYSPRWYLEGIAVYLETWMNGGLGRVIGPY
                     DEMAFRTLVRDSATIYDVVGLESEGTTIDFQTGVNSYLYGTRFVSYLALQYGNDSLLA
                     WFDRTPGSRAYFASQFRHVYGTSLDDEWSRWIAWERDWQRANLAAIRRHPTTVARPVT
                     DRALGSVSRAYYDTTNRSIYVAIQYPGQVSHIAAIDIESGRLEKIREVLGASGFYVTA
                     LAFDPASRTLFYTTNNADWRHLVSLELETGRFEVLLRDARVGDLVFNPADRSLWGVRH
                     DNGFSTLVRIPPPYDEWNQVHTLPYGRDLFDLDISPDGTILTGSMSEISGRQQLVKMA
                     TGALLQGEVTHEVLADFDEWSPSNFVFSPDGRYLFGSSYYSGVSNIFRYDFTTGETEP
                     LSNAETGFFKPVPLSADSVLVFQYTGRGFVPSMIPNEVPDSVSAIRFLGNEIAERQPD
                     VQSWEVPPVSRINLDSLTTASGSYRAVRHMKLDNLYPVVEGYRDSAGTYAVA"
BASE COUNT          393 a          825 c          816 g          432 t
ORIGIN      
        1 ccagcgacgg gttccgggag gcggtggcga gcgacgggtt ccgcgaggcc ctgacgagcg
       61 acgggttccg cgaggccctg acgagcgacg ggttccgcga ggccctgacg agcgacgggt
      121 tccgtgaggc gttggcgagc gacgggttcc gcgaggccct ggtgagcgac gggttccgcg
      181 aggccctggc gagcgacggg ttccgcgagg ccttggcgag cgacgggttc cgcgagctgc
      241 tcggcagcga tgcgttccgg accttggcgc tccgcgagtc gctgtcgcag tcgttcctgc
      301 gagaggcgca ccgcctagag cagtgatctg acgcgccgga ctcagccacc cgccggcctg
      361 gggtgggttt caacggtgat cccggcccgc tctaacgggt cccgggatca ccggttattt
      421 tcgcccccgg ccgcttccat gcgacttctc cgattcgcca cgggaaccag gcgcgcagcg
      481 gggctgcgca gccatccgcc ccgccctgtc ctgggcaggg tcgcggcctt tgtcgtgtgc
      541 gtggggacgg ctgcgcctgg cgcactcgcc gggcagttcc ggacgctcga gaccggcaac
      601 ctgcagctgg tgtattcctc tccgctccag tcccatctgg taccccaggt ggccaggagc
      661 ttcgagaact cgctacagtt ctaccacgac ctgttccagt acacgccacg tggcaaggtc
      721 ggcctgctga tgcacgacct gtggcactat ggcaacgccg gcgcccgggc gacgccggag
      781 aaccacatca ccgtcgggat cgcgccgaac cagcacgtgt acgagtcgtc gcccgccccc
      841 gaacggatgg tctccgcgct caatcacgag atggcccaca tcgtcacgat cgacaaggcc
      901 gcctcgaccg accgcttctt ccgatcgatc ttcttcggca aggtcagagc caacgccgaa
      961 gtgccgctgt ccatgcttta cggctacctg accgcgccgc ggtggtattc gcctcggtgg
     1021 tacctggagg gcatcgccgt ctatctcgaa acgtggatga acgggggtct ggggcgcgtc
     1081 atcggtccct acgacgagat ggcgttccgg accctggtgc gggacagcgc caccatctat
     1141 gacgtggtcg ggctggagtc ggaagggacg accatcgact tccagacggg ggtgaactcc
     1201 tacctctacg gcacgcgttt cgtcagctat ctggcgctgc aatacgggaa cgacagcttg
     1261 ctggcgtggt tcgaccggac cccgggcagc cgtgcctact tcgcgtcgca gttccggcac
     1321 gtgtatggca catcgctcga cgacgagtgg tcgcgctgga tcgcctggga gcgtgactgg
     1381 cagcgagcca acctcgcggc gatcaggcgt catccgacca cggtggcgcg cccggtcacg
     1441 gaccgggcgc tgggatcggt atcccgggcg tactacgaca cgacgaatcg cagcatctac
     1501 gtcgcgattc agtatccggg gcaggtctcc catatcgcgg ccatcgacat cgaatccgga
     1561 cggctcgaga agatcagaga ggtgctcgga gcgtcgggat tctacgtgac cgcgctggcg
     1621 ttcgatccgg cgtcgcggac gctgttctac acgaccaaca acgccgactg gcgtcacctc
     1681 gtctccctgg agctggaaac cggccggttc gaggtcctgc ttcgggacgc gcgggttggc
     1741 gatctcgtct tcaacccggc cgaccgttcc ctctggggcg tccggcacga caacgggttc
     1801 tccacgctgg tgcggatccc ccctccgtat gacgagtgga atcaggtcca caccctgccg
     1861 tacggcaggg atctgttcga cctggacatc tcgccggacg gcacgatcct gaccgggtcg
     1921 atgtccgaga tcagtgggcg ccagcagctg gtgaagatgg ccaccggcgc gctcctgcag
     1981 ggagaggtca cgcacgaggt cctggccgac ttcgacgaat ggtcgccgtc gaacttcgtg
     2041 ttttcgccgg acgggcggta cctgttcggc tccagctatt actcgggcgt ctccaacatc
     2101 ttccgctatg acttcacgac gggggaaacg gaacccctga gcaacgccga gacggggttc
     2161 ttcaagccgg tcccgctatc cgcggactcc gtcctcgtct tccagtatac cggccgggga
     2221 ttcgtgccct cgatgattcc gaacgaggtg ccggacagcg tgagcgcgat ccgcttcctg
     2281 ggaaacgaga tcgccgagcg acagcccgac gtgcagtcat gggaggtgcc cccggtttcg
     2341 cgcatcaacc tcgactccct cacgaccgcc agcgggtcct accgcgcggt gcgccacatg
     2401 aagctggaca acctgtatcc cgtcgtggag gggtaccggg attcggccgg cacctacgcc
     2461 gtggcc
//
LOCUS       DBNHQZ010000315         3535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_906716, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000315 DBNHQZ010000000
VERSION     DBNHQZ010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_906716"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="AC16YU_08430"
     CDS             <1..1947
                     /locus_tag="AC16YU_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6065291.1"
                     /translation="RGDVWVTSFRDLVRISGADGSVERILDRDDVPEVAAVSYARFMT
                     EDLEGRIWWVLTSPGIPTSVLRYDPDTDRFSQYVHDISDRTTWSGGSPSGITIHPEDP
                     DRIFISTYGAGLSELSVSTGRFRHYTAADGLTNQSTYEAIFDNEGMLWVPSNSGLFEF
                     DPSTGRFRRFGLEYGLQSLEFNGSSVAKSPSGELFFGGVQGFNAFFPSQLRSNDVPPD
                     VAITDFLLFNERVMPGPDSPLATTVADTKEIRLRHDQNAPSFRFAALHFKRPEANQVR
                     YRLEPQETDWVDAGERTEASYTNLKPGEYTFRVIAANSDGVWNAEGASLRLIIEPPWW
                     TRWWAYLVYALALGAAVYGIDRYQRSRIARRQRDEVRESELRHAKEIEAAYVQLQQTK
                     EQLVQQEKLASLGSLTAGIAHEIKNPLNFVNNFAEVNAELAQELDEAVERGDLDEARQ
                     LLADLRGNAEQITKHGKRADSIVRSMMQHARGESSERETVSVNDYVGEYVGLAWHGMR
                     ARDHGFSAEVIRDFAEDTGSVSVQPQEIGRVLLNLLNNAFHAVQEKAEFAGDSYAPQV
                     RVSTRRSGDVVEIRVSDNGSGIPEGLREKIFEPFFTTKATGEGTGLGLSLSHEIVTQG
                     HGGSLTVGSSNGGGAEFVVRLPAR"
     gene            complement(1953..>3535)
                     /locus_tag="AC16YU_08435"
     CDS             complement(1953..>3535)
                     /locus_tag="AC16YU_08435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6065292.1"
                     /translation="TLESASVTQDGAVLGTAAYMAPEQALGSDSDHRVDVWAVGAILY
                     ELLSGRRPFEGAYPQAVIYGILNADPEPLSGLRPGLPLQLVQAVERALAKDPNRRHAT
                     AGDFRDALRAVSDRKAARSQARDLIEETEAHVPAPAVPDADTGVLHLLCVDDEPELEL
                     LMQQRFRKRIRAGEWSFTFATDGRDALRKLESHPEIGVVLTDLNMPGMDGLALLGELA
                     GLDRVIRTVVVSAYGDMEKIRTAMNRGAFDFVTKPVDFGDLDATIQKAARDLEIYRRA
                     LRSQQQLVSIRQELDIASRIQDAILPVTFPQVDGLEVYGFSAPAQEVSGTFYDAFPVE
                     GGRLGLIAGDAKSRGVTAALLMAMGQTFVKSFLQQGQEPHACLMQLNRMLFADGLPSV
                     SLQVVAAVADPARGTLTYANAGHTDPMLRRADGSVEILKPRHAAIWSSGDSRLESTVT
                     KLGPGDAVLLPTRGLTEAVNSAGSPFTVERLAATLREAEDARPTQLIRHVVRAIHDYA
                     EDEEPKEDLTLLAVRRNP"
BASE COUNT          579 a         1175 c         1235 g          546 t
ORIGIN      
        1 cgcggcgacg tctgggtgac gtcgttccgg gacctggtcc gcatttccgg cgccgacgga
       61 tcggtcgaac ggatactgga ccgcgacgac gtgcccgagg tggcggccgt ctcgtacgcc
      121 aggttcatga ccgaggacct ggaaggacgc atatggtggg tgctcacgtc gcccggcatc
      181 ccgacctcgg tgctccgcta cgatccggac acggaccggt tcagccagta cgtccacgac
      241 atctcggatc ggaccacgtg gagcggaggc tcgccgtcgg gcatcacgat ccatcccgaa
      301 gacccggacc ggatcttcat ctccacgtac ggagccggac tatccgagct cagcgtgagc
      361 acgggccgct tccggcacta cacggcggcc gacgggctga ccaaccagtc gacgtacgag
      421 gcaatcttcg acaatgaggg gatgctgtgg gtgccgtcga actcgggact cttcgaattc
      481 gacccctcga cgggcaggtt caggcggttc ggactcgaat acggactgca gtccctggaa
      541 ttcaacggaa gctcggtggc gaaatccccg tccggagagc tcttcttcgg tggggtccag
      601 ggattcaatg cgttcttccc gtcccagctt cggtccaacg acgttccgcc cgacgtggcg
      661 atcaccgatt tcctgctgtt caacgagcgg gtaatgccgg gaccggattc cccgcttgcg
      721 acgaccgtgg ccgacacgaa ggagatccgt cttcggcacg accagaacgc tccgtccttc
      781 cggttcgccg ccctgcactt caagcgtccc gaggccaacc aggtccggta ccgtctggag
      841 ccgcaggaaa ccgactgggt ggatgcaggc gaacggaccg aggcgtccta cacgaacctg
      901 aagcccggcg agtacacgtt tcgtgtcatc gcggcgaaca gcgacggcgt atggaacgcg
      961 gagggggcgt cgctgagact catcatcgaa cccccctggt ggacccgatg gtgggcatac
     1021 ctggtgtatg ccctcgcgct gggcgctgcc gtgtacggca tcgatcgcta ccagcgctcg
     1081 cgcatcgccc ggcggcagcg tgacgaagtg cgcgagagcg agctccggca cgcgaaagag
     1141 atcgaggccg cctacgtgca gctgcagcag acaaaggagc agctcgtgca gcaggagaag
     1201 ctcgcgtcgc tcggctcgct cacggccggt atcgcccacg agatcaagaa cccgctcaat
     1261 ttcgtcaaca acttcgccga ggtgaacgcg gagctcgccc aggagctgga cgaggcggtg
     1321 gagcgcggcg acctggacga ggcgcgccag ctcctggccg acctccgggg caacgccgag
     1381 cagatcacga agcacggcaa acgggccgac tcgatcgtcc ggtccatgat gcagcacgcg
     1441 cgcggcgaat cgtccgagcg cgagacggtg tccgtgaacg attacgtcgg agagtacgtc
     1501 ggactggcct ggcacggcat gcgggcccgc gaccacggct tctcggccga ggtgatccgg
     1561 gacttcgcgg aagacaccgg atcggtctcc gtgcagccgc aggaaatcgg gagggtgctc
     1621 ctgaacctcc tgaacaacgc cttccacgcc gtccaggaga aggccgagtt cgccggcgac
     1681 tcgtacgcgc cgcaggtccg ggtttcgacc cggcgtagcg gcgacgtcgt cgagatccgt
     1741 gtgtccgaca acggatcagg cattccggag gggctccggg agaagatctt cgagcccttc
     1801 ttcacgacga aggccacggg cgagggcacg gggctggggc tgtccctgag ccacgagatc
     1861 gtgacccagg gccacggagg ctcgctgacc gtgggatcgt cgaacggcgg cggcgccgaa
     1921 ttcgtggtgc ggcttccggc ccgctgacgc cgctacgggt tccggcggac ggccaggagg
     1981 gtcaggtcct ccttcggctc ctcgtcctcc gcgtagtcgt ggattgcgcg caccacgtgc
     2041 cggatgagct gggtgggccg cgcgtcctcg gcctcgcgga gggtcgcggc gagccgctcc
     2101 acggtgaacg gcgaccccgc ggaattgacg gcttcggtca ggccgcgcgt gggcaggagc
     2161 accgcgtcgc ccgggccgag cttcgtcacg gtcgactcga gtcgcgagtc gcccgatgac
     2221 cagatcgcgg cgtgccgggg cttgaggatt tccacgctgc cgtccgcccg gcgcagcatc
     2281 gggtcggtgt ggccggcatt ggcatacgtg agcgtgcctc tcgccgggtc ggccaccgcc
     2341 gccacgacct ggagcgagac cgacggcagg ccgtccgcga acagcatgcg gttcaactgc
     2401 atgaggcagg cgtggggctc ctggccctgc tggaggaagc tcttcacgaa ggtctgaccc
     2461 atggccatca acagcgccgc ggtcaccccc cgggacttcg cgtcgccggc aatgagcccg
     2521 agccggccac cctcgacggg gaatgcgtcg taaaacgtgc cgctcacctc ctgggcgggc
     2581 gccgagaatc cgtagacctc gaggccgtcc acctgcggaa acgtgacggg caggatcgcg
     2641 tcctggatcc ggctcgcgat gtcgagctcc tgccggatcg aaaccagctg ctgctgcgag
     2701 cgcagggcgc gccggtagat ctcgaggtcc cgggccgcct tctggatcgt ggcgtcgagg
     2761 tccccgaagt cgacgggctt cgtgacgaag tcgaacgccc cgcggttcat cgccgtccgg
     2821 atcttctcca tgtcgccgta cgcggagacg acgacggtcc ggatcacccg gtccaggccc
     2881 gcgagctcgc cgagaagcgc gagcccgtcc atgccgggca tgttcaggtc ggtcaggacc
     2941 acgccgatct ccggatggct ttcgagcttc cgcagggcgt cccggccgtc ggtcgcaaag
     3001 gtgaagctcc actccccggc gcggatccgc ttgcggaagc gctgctgcat gaggagttcg
     3061 agctcgggct cgtcgtccac gcacaggagg tggagcacgc cggtgtcggc gtccggcacg
     3121 gcgggcgctg ggacgtgcgc ttcggtctcc tcgatcaggt cgcgggcctg ggaacgcgcc
     3181 gccttgcggt cggacacggc ccgcagcgcg tcccggaagt cccctgccgt ggcatggcgc
     3241 ctgttcggat ccttggcgag ggcgcgctcg accgcctgaa ccagctggag cgggaggcct
     3301 ggacggagcc cggacagcgg ctcggggtcg gcgttcagga tgccgtagat gacggcctgg
     3361 gggtaggcgc cctcgaaagg acggcgcccg gagagcagct cgtacaggat cgccccgacc
     3421 gcccagacgt cgacccggtg gtcggagtcc gagccgagcg cctgttcggg agccatgtag
     3481 gcggccgtgc ccaggacggc gccgtcctga gtcacgctgg ccgattcaag ggtct
//
LOCUS       DBNHQZ010000316         8900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_364653, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000316 DBNHQZ010000000
VERSION     DBNHQZ010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_364653"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..711
                     /gene="hslV"
                     /locus_tag="AC16YU_08440"
     CDS             145..711
                     /gene="hslV"
                     /locus_tag="AC16YU_08440"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR6065293.1"
                     /translation="MNTRDTGRMRATTVVGVHHSGRVALGSDGQATLGNTVMKHRAQK
                     VRQLHNGKILAGFAGATADAFTLFERFEEKLQQFGGNLTRSAVELAKDWRTDRYLRRL
                     EALLAVAAPDRLLLISGTGDVIEPDDNIVAIGSGGPYALAAARALVAHAPSLSAREIV
                     EESLGIAADICIYTNHHLTVLELDASGD"
     gene            752..1762
                     /locus_tag="AC16YU_08445"
     CDS             752..1762
                     /locus_tag="AC16YU_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065294.1"
                     /translation="MTARRHRGPAASDTPQGIILVDYDNLVDALAERTSRSDQPHRFL
                     LAMLGELRSYLESQMGLRSARTIAFGDFASHPVDAGTILTDLGTAGVEARHVSGAVQR
                     SDGACTLVLTAAGVLHARADLVAVVVLTGDNWYVPVVQAVQEAGRFALVAALDLPPNL
                     ATLHPSIGDAYLNARFLLDPRAREALSDDDAGGSARSAREPSGASVQERAPTETSPID
                     DPGAVRALEVLEAHFGQYEEVYLTPLLRKLTESLDEEDGEPKDLVNMLQEAGAVWLEK
                     RRGFPYDYTVLLVNRSHPDVVRTMESVASDERPGDASDDYDEGNYDDDAFDDQYDVES
                     RS"
     gene            1856..3244
                     /gene="hslU"
                     /locus_tag="AC16YU_08450"
     CDS             1856..3244
                     /gene="hslU"
                     /locus_tag="AC16YU_08450"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR6065295.1"
                     /translation="MQRELTPHQIVAELDRYIVGQTEAKKSVAIALRNRWRRLNASED
                     MRDEIMPNNIIMIGPTGVGKTEIARRLARLAGAPFIKVEATKFTEVGYVGRDVDSMIR
                     DLTDIAINMVREEYADGVQDRARELAEDRILEILIPPPPVPKAGFGIAEHSVVADAEP
                     GEAEMRSRTRERFRDMLRRSELDSREIEVEVSADGSSSMLQVFGPMGLEEMGLNLQDL
                     LGGAGGKRRKKRRMRIEEARTVLAQEEAQKLIDMDKVQREALERVQQAGIVFIDEIDK
                     VSIRSAAGRGGPDVSREGVQRDLLPIVEGSGVMTKYGLVKTDHILFIASGAFHVSKPS
                     DLIPELQGRFPIRVELHSLTVDDFYKILTIPRNALLKQYQALLASEGVDVTFTDGAVH
                     AIARIAAQVNEDVENIGARRLHTILTTLLEELLFDVPDGYDGGAVSVDEAMVEARLSG
                     IAMNRDLSQYIL"
     gene            3261..4184
                     /locus_tag="AC16YU_08455"
     CDS             3261..4184
                     /locus_tag="AC16YU_08455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6065296.1"
                     /translation="MTVRPASDADAWARWDRVRDRDDPFGSAAYARALGLAGAPLLVC
                     GDDGGPAALVAARSTGPFRRAFVPPLSPYTPVALGVGDATTALGRLASGLADAFDDVV
                     LLLPPAVADVRALQWAGWTVEPRHTYRLDPSVAADPGTWSENPRRTLRQHGSAFRIEE
                     GERFVPDVASLCAASYGRHDRSGPLPEASMHTMAAHMAEAGLVRVLAALDPDGAVRAA
                     VALLCGPTACYYWMAGSEPGPAMTVLLASVFRMLADDGVPVLDLVGANTPSIAEFKRR
                     FGPDLVRYHVARRTPSRILRLAVAFRSAFRA"
     gene            4188..5774
                     /locus_tag="AC16YU_08460"
     CDS             4188..5774
                     /locus_tag="AC16YU_08460"
                     /inference="COORDINATES: protein motif:HMM:NF042614.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HIR6065297.1"
                     /translation="MESDVRVSWRPALAAALLAPALHLARFGYSVGYSDQDEFLPWLL
                     HRLDPSLLASDWLVGVQESGFNVRSGMLALLEPFAAWLGVEPALAVLYGVAWLILGLA
                     VYDFMHGVSGSAAAALAGTVVTLAAIPRWTLGGSAMTATMLVPSMAAWSLGVAALALH
                     FRGRSLLPGFLLGVAAWLQPVDAALVALPLAIAGIARGGPRGSWASGLVRDTLRLAVP
                     AGLLVLPLAVLYILGRPAPPSPGFEPGFLEILTRFRAPHHFLPAAFPAGDWIQVLVLT
                     VAGAAGFALWRRTAERGPTMYVFALLVTAAAVVALGFVSLAIPAVALLQPFRITVLSG
                     LVLAALAGAGAAALVRGRSPDAIGRLDRAVLRPAVPAVAALALALPLVLGSPRTAADA
                     DVAAAADWLRTRSESRSTVAVPPSTTGFRYRSGRSVVVTFKAIPFGPADLAAWYRRLS
                     DLAPGAVRPSGREVAAVLSELDAAYELLSERDLDEIALRYGVSWAVRRTAIDPEPVHW
                     ALESRHGSVLVYGRRPGDAR"
     gene            5771..7222
                     /locus_tag="AC16YU_08465"
     CDS             5771..7222
                     /locus_tag="AC16YU_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6065298.1"
                     /translation="MKRTGKPADGRRQGLRGPVLTLLSGSAVAFLVGYVAKLVLLRLY
                     DPADFGLYEFIIAIIGVILPVSSLRYEDAVVLPESDDEARNVLGAAALVGAASVLMSL
                     ALIPLSGVVARLVGEPGVAAWIWTVPAILAAMRLSRLGELWLMRRERFGRVSVANVTQ
                     SVTMTAVRIAAGVRAAGAGGLLAGFGIGHGIAALVNGSAALLDVVRIPGRASLAGIRR
                     AMHRYRRFAVYSAPATLVAALATRLPFLLLLYYFDREVLGHFGQAFNVLYVPLALVAA
                     AVGQVFFVRAADAVKRETLPALAAAVHRRLVLIAFLPVVSVAAAGPDVFEALFGPVWR
                     EAGEYAGWIGAWILFTSIASPLTRLFDVLERQRADLVVNAVMLAVVTAALVVGGASGS
                     VRTALVTVGAAGAIVRIGQIVVSLRLAGLRFADILAPYLRHGRLAIAAGAVLLLVRLA
                     GLPLLTTGAFFLVLAAFGVHTARREGLIPWALP"
     gene            7219..8094
                     /locus_tag="AC16YU_08470"
     CDS             7219..8094
                     /locus_tag="AC16YU_08470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6065299.1"
                     /translation="MTRHSVRVLAALLLLAASGCARDTGTPAGAAVEPLRVVTFNIRL
                     NLASDGQDAWPLRRDWVASILDSLDADVIGLQEALVGQVRDLDSALVSHDRVGVGRDD
                     GHEAGEFSPLFYRSDRLEMLDSGTFWLSETPDSAGSVGWDAALPRIASWARMRMTRGG
                     REILVVNTHFDHMGVEARRRSAALLHERFVAAANPAPLVLMGDFNVTDTTAAYRVLTV
                     PDHGLVDAWKAAGAAGPTDTFTGFEASGQPGPRIDFVFVSRDWDVVSARAEHLERNGR
                     HPSDHRPLVAELRLR"
     gene            complement(8155..8226)
                     /locus_tag="AC16YU_08475"
     tRNA            complement(8155..8226)
                     /locus_tag="AC16YU_08475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8192..8194),aa:Gly,seq:gcc)
     gene            8460..>8900
                     /locus_tag="AC16YU_08480"
     CDS             8460..>8900
                     /locus_tag="AC16YU_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HIR6065300.1"
                     /translation="MAQIIDGKAVAATIRLEVADRVRTHVNAGNRPPSLRVVLVGDDP
                     ASASYVRGKSRACEETGIDSETLTFAGDLAERELLRTIHLLNHDDSVDGILVQLPLPR
                     HVDEQKVLRAISPRKDVDCFHPENVGRLVLGLPGPRPATPQGVLM"
BASE COUNT         1207 a         3181 c         3041 g         1471 t
ORIGIN      
        1 tcgagacgct ggcgcttcac aggcgctgaa atgaccattg cgggaggccc cgcgacatcc
       61 gtaccttgcg gggtctattc ggcgtggcgt ctgtattgcg tcgtcatgcg ttcactgtct
      121 catcgacgtc atccttctat tgtcatgaac acgcgggata ccggccgcat gcgcgccacc
      181 acggtcgtgg gagtgcatca cagcgggcgt gtggccctgg gatcggatgg acaggccacc
      241 ctgggcaaca cggtcatgaa gcaccgcgcg cagaaggtcc gccagctgca caacggcaag
      301 atcctggccg gattcgccgg cgcgacggcc gacgcgttta ccctgttcga gcgcttcgag
      361 gagaagctgc agcagttcgg gggcaacctg acgcgaagcg ccgtcgagct ggccaaggac
      421 tggcgcaccg accggtacct gaggcgcctg gaggccctgc tcgccgtcgc ggccccggac
      481 cgcctgctgc tcatttcggg cacgggtgac gtcatcgagc ccgacgacaa catcgtggcg
      541 atcggatcgg gcggaccgta cgccctcgcg gctgcgaggg cgcttgtcgc gcatgccccc
      601 tcgctgtccg cgcgggagat cgtcgaagag tcgctgggaa tcgcagccga catctgcata
      661 tacaccaatc atcacctcac cgtgctggaa ctcgatgcct caggggattg acccctgttc
      721 cgggtccgtt cctgacacgg gaatcaccga aatgaccgct cgccgtcatc gcggccccgc
      781 cgcttccgat accccccagg gcatcatcct ggtggactac gacaacctcg tcgacgcgct
      841 cgccgaacgc accagccgca gtgaccaacc gcaccgtttt ctgctggcca tgctcggcga
      901 gctccggagc tacctcgagt cccagatggg ccttcgctcc gcccggacca tcgctttcgg
      961 cgatttcgca tcgcatccgg tagacgcggg tacgattctg acggacctgg gcaccgccgg
     1021 tgtcgaggca cggcacgtgt cgggcgccgt gcagcgaagc gacggggcct gcaccctggt
     1081 gctgacggcg gcaggcgtgc tccacgcgcg ggccgacctg gtggccgtcg tcgtgctgac
     1141 gggggacaac tggtacgttc ccgtcgtgca ggccgtccag gaggccggcc gcttcgcgct
     1201 ggtcgcggcg ctcgacctgc cgccgaacct cgccaccctg cacccctcca tcggtgacgc
     1261 ctaccttaac gcccgcttcc tgctggatcc ccgtgctcgc gaggccctct cggacgacga
     1321 tgccggggga tcggcccgga gcgcgcgcga gccgtcgggc gcctcggttc aggagcgcgc
     1381 cccgaccgaa acctcgccta tcgacgatcc gggagccgtg cgtgcgctcg aggtcctgga
     1441 ggcccatttc ggccagtacg aggaagtgta tctcactccc ctcctccgga agctgaccga
     1501 atcgctggac gaggaggacg gcgagccgaa ggatctcgtg aacatgctcc aggaggccgg
     1561 ggcggtctgg ctcgagaaga ggcggggctt tccgtacgac tacaccgtgc tgctcgtcaa
     1621 ccgctcccac ccggacgtcg tccggaccat ggagagcgtc gcgtcggatg agcggccggg
     1681 cgacgcgtcg gacgactacg acgagggcaa ctatgacgac gacgccttcg acgaccagta
     1741 tgacgtcgag tcgcggtcct gaccgacggc cctgtcaccc ggccggtccg cgccgcccgg
     1801 gcactcgccc gccctgcacg cgtttccgcg gtgtcaccgt acccgattcc tgcccatgca
     1861 gcgtgaactg accccccacc agatcgtcgc ggagctcgac cggtacatcg tcggacagac
     1921 cgaggcgaag aagagcgtgg ccatcgcgct tcgcaaccgg tggcgtcgcc tgaacgcctc
     1981 ggaggacatg cgggacgaga tcatgccgaa caacatcatc atgatcggtc ccacgggggt
     2041 cggcaagacg gagatcgccc ggcggctggc ccggctcgcc ggggccccgt tcatcaaggt
     2101 cgaggcgacg aagttcaccg aggtcggcta cgtgggccgg gacgtggaca gcatgatccg
     2161 cgacctgacc gacatcgcca tcaacatggt ccgcgaggaa tacgcggacg gcgtacagga
     2221 ccgtgcccgc gagctcgcgg aggaccgcat cctggaaatc ctcatcccgc ccccgcccgt
     2281 tccgaaggcg ggattcggca tcgccgagca ttccgtggtc gccgatgcgg agccgggcga
     2341 ggccgagatg cgcagccgca cgcgcgagcg gttccgggac atgctgcgcc gctccgagct
     2401 cgattcccgc gagatcgagg tcgaggtgtc ggcggacgga tcgtcctcga tgctgcaggt
     2461 attcggtccg atgggcctcg aggagatggg actgaacctg caggacctgc tgggcggcgc
     2521 gggcggaaaa cgccggaaga agcgccggat gcgcattgag gaggcccgca cggtcctcgc
     2581 ccaggaagag gcgcagaaac tcatcgacat ggacaaggtc cagcgggagg cactcgaacg
     2641 cgtccagcag gcgggcatcg tgttcatcga cgaaatcgac aaggtgtcca tccgctcggc
     2701 cgccggccgc ggcggaccgg acgtatcgcg cgaaggcgtc cagcgcgacc tgctcccgat
     2761 cgtcgagggc tccggcgtca tgaccaagta cgggctggtc aagaccgacc atatcctgtt
     2821 catcgcgagc ggggcctttc acgtctccaa gccgagcgac ctgatccctg aactgcaggg
     2881 gcggttccca attcgcgttg aactgcatag ccttacagtt gacgacttct acaagattct
     2941 gacgatcccg cggaacgccc tgctcaagca gtaccaggcg ctgctggcgt cggagggcgt
     3001 ggatgtcacc ttcacggacg gcgcggtgca cgcgattgcg cggatcgccg cccaggtgaa
     3061 cgaagacgtc gagaacatcg gcgcccggcg gctgcacacg atcctgacca cgctgctcga
     3121 ggagctgctc ttcgacgtcc cggacgggta cgacgggggg gccgtgtccg ttgacgaggc
     3181 catggtggaa gcccgtctga gcggaatcgc catgaacagg gacctgagcc agtacatcct
     3241 ctgagagccc cgatcgcccg atgaccgtcc ggcccgccag cgatgcagac gcctgggccc
     3301 ggtgggaccg ggtgcgggat cgcgacgatc ccttcggaag cgcggcgtac gcgcgcgctc
     3361 tcgggctcgc gggcgctccg ctcctggtgt gcggggacga cggaggtccg gccgcgctgg
     3421 tggccgcccg ctccacgggg ccgttccgac gggcattcgt tccgccgctg tccccgtaca
     3481 cccctgtcgc cctcggcgtg ggcgacgcca cgacggcgct cggccggctc gcctccggcc
     3541 tggcggacgc gttcgacgac gtcgtgctgc tgctcccccc cgccgtcgcg gacgtccggg
     3601 cgctccagtg ggccggttgg accgtcgagc cgaggcatac gtaccggctg gatccctcgg
     3661 tcgcggccga tcccggcaca tggtcggaga acccaaggcg cactctcagg cagcacggct
     3721 cggccttccg gatcgaagag ggggagaggt tcgttccgga cgtcgcgtcg ctgtgcgcag
     3781 cctcgtacgg gcgtcacgac cggagcggcc cgctcccgga ggcgtccatg cacacgatgg
     3841 cagcccacat ggccgaggcc gggctggtcc gggtgcttgc cgccctggac ccggacggcg
     3901 ccgtgcgggc cgccgtcgcc cttctctgcg gacccacagc ctgctactac tggatggccg
     3961 gaagcgagcc cggcccggcg atgacggtcc tcctggcctc cgtcttccgg atgctcgcgg
     4021 acgatggcgt cccggtcctg gatctcgtcg gcgccaacac gccttcgatc gccgagttca
     4081 agcgccggtt cggtcccgac ctcgttcgct atcatgtggc gagacggacg ccgagccgaa
     4141 tcctgcgcct ggcggtcgcc ttccgctccg ctttccgagc ctgagccatg gagtctgacg
     4201 tacgggtatc ctggagaccg gcgctggccg cggccctgct ggcgcctgcg ctccacctgg
     4261 cccggttcgg atactcggtc gggtacagcg accaggacga gtttcttccg tggctgctgc
     4321 accgcctgga cccctcgctg cttgcctcgg actggctcgt gggggtgcag gagtcggggt
     4381 tcaacgtgcg atccggtatg ctcgcgctcc tcgagccgtt cgccgcgtgg ctcggcgtgg
     4441 agccggcgct ggccgtgctc tacggcgtgg cctggctgat cctcggcctc gcggtctacg
     4501 atttcatgca cggcgtgtcg gggtccgccg ccgccgcgct tgcgggcacc gtcgtcacgc
     4561 tcgccgccat accgcgctgg accctgggcg gcagtgcgat gacggccacg atgctcgtgc
     4621 ccagcatggc ggcctggagc ctgggcgttg ccgcgctggc cctgcacttc aggggtcgca
     4681 gcctgctgcc gggatttctc ctgggtgtgg cggcgtggct tcagcccgtc gatgccgcgt
     4741 tggtcgcact cccgctggcc atcgccggca tcgcccgggg agggccgagg ggctcgtggg
     4801 cgtcgggcct cgtccgggac acgctgcgcc ttgccgtacc ggccggactg ctcgtactgc
     4861 cgctcgccgt gctctacatc ctggggcgac ccgcaccgcc gtccccgggc ttcgagccgg
     4921 gcttcctgga gatcctgacc cgcttcaggg ctccacatca cttcctgccg gccgcgtttc
     4981 ccgccgggga ctggatccag gtccttgtcc tcacggtcgc gggagcggcg ggcttcgccc
     5041 tatggcgacg gacggccgaa cgcggaccca cgatgtatgt cttcgccctg ctcgtcacgg
     5101 ccgccgccgt cgtggcgctg ggtttcgtga gtctggcgat tcctgcggtc gccctgctgc
     5161 agccgttccg gatcacggtg ctgtcgggcc tcgtgcttgc cgccctggcc ggcgccggag
     5221 cggccgccct cgtgcgcggc cggagcccgg acgcaatcgg gaggctggac cgggccgtcc
     5281 tccgtcccgc cgtccccgcg gtcgccgccc ttgcgctcgc cctgcccctg gttctgggtt
     5341 cgccccggac cgcggcggac gccgacgtcg ctgccgcggc cgactggctc cgcacccggt
     5401 cggagtcccg gtccacggtc gcggtgcccc cgtccacgac gggattccgg taccggtcgg
     5461 ggcgctccgt cgtggtgacg ttcaaggcga ttccgttcgg gccggcggac ctggccgcgt
     5521 ggtaccggcg actctccgac ctggcgccgg gcgccgtcag gccgtcgggg cgcgaggtgg
     5581 ccgccgtcct ctcggagctc gacgccgcct acgagctgct gtccgagcgg gatctcgacg
     5641 agatcgccct tcgctacggc gtgtcgtggg cggtccgccg gaccgcgatc gatcccgaac
     5701 ccgtgcactg ggcgctggag agccgacacg gctccgtcct cgtgtacggc cggcgaccgg
     5761 gagacgctcg atgaagcgca cgggaaaacc ggccgacggg cgaaggcagg gtctccgggg
     5821 acccgtgctc acgctcctgt ccgggtccgc ggtcgcgttc ctggtcgggt acgtggccaa
     5881 gctcgtcctg ctccgcctgt acgatccggc ggatttcggc ctgtacgagt tcatcatcgc
     5941 gatcatcggc gtgatcctcc cggtgtcctc gctccggtac gaggatgcgg tcgtgctccc
     6001 ggagtccgac gacgaagccc ggaacgtgct cggcgcggcc gccctcgtcg gcgctgcttc
     6061 cgttctcatg agcctggcgc tcataccgct ctcgggcgtc gtcgcgcgcc tggtgggcga
     6121 gcccggagtg gccgcgtgga tctggacggt tccggccatc ctggcggcca tgcggctgtc
     6181 ccgactcggc gagctctggc tgatgcgccg ggagcggttc ggccgcgtct cggtcgccaa
     6241 cgtgacgcag tcggtcacca tgaccgccgt ccgcatcgcg gccggcgtcc gggcggccgg
     6301 ggccggcggc ctcctggccg gcttcggtat cgggcacggc atcgccgcgc tcgtcaacgg
     6361 ttcggccgcg ctcctggacg tcgtgcgcat tcccggccgc gcctcgctcg cgggcatccg
     6421 gcgcgcgatg caccgatacc gccggttcgc ggtgtattcc gccccggcca ccctcgtcgc
     6481 ggccctcgcc acccggctcc ccttcctgct cctgctgtac tacttcgacc gggaggtcct
     6541 cggccacttc gggcaggcgt tcaacgtgct gtacgtaccg ctcgcgctgg tggccgcggc
     6601 cgtcggacag gtgtttttcg tccgagcggc ggatgccgtg aagcgggaga ctctgcccgc
     6661 cctcgcagcc gccgtgcacc gccggctggt cctcatcgcc tttcttccgg tcgtgtccgt
     6721 tgcggcggcg ggccccgacg tcttcgaggc cctgttcggt cccgtatggc gcgaggcggg
     6781 cgagtacgcc gggtggatcg gggcctggat cctgttcacg agcatcgcct ctccgctcac
     6841 ccggctgttc gacgtcctgg agaggcagcg cgccgacctc gtcgtcaacg cggtcatgct
     6901 cgccgtcgtg accgccgcgc tcgtggtcgg aggagcgtcc ggctccgtga ggacggccct
     6961 cgtcacggtc ggagcggccg gtgcgatcgt ccggatcggt cagatcgtgg tgtcccttcg
     7021 ccttgcaggc cttcggttcg ccgacatcct ggccccttac ctgcggcacg gccgtcttgc
     7081 gatcgccgcg ggcgccgtgc tgctcctcgt gcgcctggcc gggctgcccc tcctcacgac
     7141 cggcgcgttt ttccttgtcc tcgcggcctt cggcgtgcat acggcgcgcc gggaaggcct
     7201 catcccctgg gccttgccat gaccaggcac tccgtccgcg ttctcgccgc cctcctgctc
     7261 ctcgccgcat ccgggtgcgc ccgggatacc gggacaccgg cgggggcggc cgtcgagccg
     7321 ctgcgggtgg tcactttcaa catccggctc aaccttgcct cggacggtca ggacgcctgg
     7381 cccctgcgca gggactgggt cgcgtccatc ctggacagcc tggacgcgga cgtcatcggc
     7441 ctccaggagg cgctggtcgg ccaggtgcgc gacctggatt cagcgctcgt ctcccacgac
     7501 cgggtcggtg tcgggcggga cgacgggcat gaggcagggg agttctcgcc gctcttctac
     7561 cggtccgacc ggctcgagat gctggactcg ggcacgttct ggctctcgga gacgccggat
     7621 tcggcgggca gcgtcgggtg ggacgccgcc cttccccgca tcgcctcgtg ggcacggatg
     7681 cgcatgaccc ggggcggccg cgagatcctg gtcgtgaaca cccacttcga ccacatgggc
     7741 gtggaagctc ggcgacgcag cgctgcgctg cttcacgagc ggttcgtggc cgccgcgaac
     7801 cctgcgccgc tcgtcctcat gggtgatttc aacgtcaccg acacgaccgc agcctaccgc
     7861 gtgctcaccg ttccggacca cgggctcgtc gatgcctgga aggccgcggg cgcggcggga
     7921 ccgacggaca cgttcaccgg gttcgaagcg tccgggcagc cggggccgcg catcgacttc
     7981 gtcttcgtgt cccgggactg ggacgttgtc tccgcacggg cggagcacct ggagcggaac
     8041 ggccgccatc cgtcggatca ccgtccgctc gtcgccgagc tgcgcctccg ctgagcggca
     8101 cgccggaagg cgcccggaaa agaaaggccc cgaatcatgg gatccggggc cctgagcggg
     8161 tgatgagatt tgaactcacg accctcacgt tggcaacgtg atgctctacc actgagctac
     8221 acccgcgtcg tagagaaaag aattatactg ccgtttgcat acccctgtca agccttttcg
     8281 gcctattcac gcgacccgga ccggcatgtg aacgcccgga gaaagccgcc gggtgaccgc
     8341 gcgcaaggcc ccatgcggac tgttgcccga gggccggatg ccctagcttg tcgggacctg
     8401 ccgcaccgcc cgatccgcgg ccacccgccc tgccgtacaa cactctccga ggattccccg
     8461 tggcccagat catcgacgga aaggctgtgg ccgccacgat ccgcctggaa gtcgcggacc
     8521 gcgtccgtac ccacgtgaat gccggcaacc gcccgccctc gctccgggtg gtgctcgtgg
     8581 gcgacgaccc cgcgtcggcg tcgtacgtcc gcggaaagag ccgggcctgc gaggagaccg
     8641 ggatcgacag cgagaccctg accttcgccg gcgaccttgc ggagcgagag ctgctccgga
     8701 ccatacacct cctcaaccac gacgattcgg tcgacggcat cctcgtgcag ctgccgctcc
     8761 cgcgtcacgt cgacgagcag aaggtgctcc gcgccatctc gccccgaaag gacgtggact
     8821 gcttccatcc ggagaacgtc gggcgcctgg tgctcggcct gccggggccc aggcccgcca
     8881 cgccgcaggg cgtgttgatg
//
LOCUS       DBNHQZ010000317         4481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2667513, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000317 DBNHQZ010000000
VERSION     DBNHQZ010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2667513"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="AC16YU_08485"
     CDS             <1..361
                     /locus_tag="AC16YU_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6065301.1"
                     /translation="NAEAGGTIWADVQAQVPGAAVHSSGRGGTVHEVRIAEGSHLRTI
                     LGRDTAQVNTHHIQAVKEIGDGFRVSAVSPDGVVEGIETADASVIGVQFHPERQMDDL
                     LPLFVDLVRRAAQGSAT"
     gene            501..926
                     /locus_tag="AC16YU_08490"
     CDS             501..926
                     /locus_tag="AC16YU_08490"
                     /inference="COORDINATES: protein motif:HMM:NF039992.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HIR6065302.1"
                     /translation="MFAELRSLDTGPRTLRRFGLAVGGVLFAAGAWFAWRSGTPAAPV
                     PAILLAAGSLLVLSGSVAPTALRWPFLAWMGLALVLGAVMTRVILTLVYFLVVTPVGL
                     AMRALGRDPMHRRPDPGADTYWIRRDPDDDPRERLTRYW"
     gene            complement(933..3158)
                     /gene="ppk1"
                     /locus_tag="AC16YU_08495"
     CDS             complement(933..3158)
                     /gene="ppk1"
                     /locus_tag="AC16YU_08495"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6065303.1"
                     /translation="MSTPTTRPDVGIQPEATNGEPAPAAVAGAVRVVVPRAVSPLPVP
                     KRASLSDPRLYFNRELSWLDFNWRVLYQALDERNPVLERVRFLAITASNLDEFFRKRM
                     GGLKRQAAAGVARLSPDGRTPAEQIALCQQAVQPMVRRVSEVWLRVLRPALAANGIRI
                     LDHADLDDQQKAYLHEQFIAHIFPILTPLAVDPGHPFPFISNLSLSLAVTMRHPKRGT
                     EHFARLKVPTARGRWIRLPGGLDFVPVEQVIANNIDDLFRGMKVTSVHAFRITRNADI
                     RRDEEEADDLIEMIADELRERRFAPIVRLEVEAGMPPDVRTLLMRELGLKEHDVYEAP
                     GEIDLSDLHAIADLDRPDLRYEPWEPVVPERLRHVGEAADREDIFTSIRAGDLLVHHP
                     YESFAASVLRFVEEAAEDPAVIAIKLTLYRTSDESPIVDALIRAAERGKQVAVLVEVK
                     ARFDEMNNIEWGQMLEKAGVHVTYGLVGLKTHTKTTLVIREEADGLRAYCHIGTGNYN
                     PKTARLYTDLGLFTSARDVGFDIINLFHYLTGYAPEQHYRRLIVAPRDMRDRFVEMLH
                     AEIGHQKATGNGRVIAKMNALDDPVMIRELYRASEAGVQIDLIVRGHSRLRPGLPRFS
                     ENIRVISILGRFLEHSRIYYFRNNDRPRVLIGSGDWQRRNLDDRVEATVEIDDPALRA
                     RLIRSLEIALRDRRSAWELDPNGVYGLRWPGEGDDQRGVQEILMDLATAKRGERGWDI
                     A"
     gene            complement(3155..>4481)
                     /locus_tag="AC16YU_08500"
     CDS             complement(3155..>4481)
                     /locus_tag="AC16YU_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6065304.1"
                     /translation="EGGGWYDRYRARLIFPILSHVGKVLGFGGRILVEDPAQPKYVNS
                     PETAVYHKSRVLYGLYQAKQAIRAKEEAILVEGYTDVIALHQAGVGHVVASSGTALTP
                     DQVRLVSRYARRVLLLYDADDAGAKATARGIDLCLEGGLVPYVVTLPDGEDPDTFVRN
                     QGGDAFEAYLAGHRQEFVAYLHERARLEGTLDAPESRAAAQHAVVRTIGRIADPLLRE
                     SYMQQAASVLDVPDVYLRRALERERRQGERRPAASDRGPERPRDSAPAGAGASAETGR
                     PAPGEPLPEEKTLLRLMFENGSEMVAFVLSRTSLDEFTEGPSRETVSHLVRLYQEGRL
                     DRRPFLDGTFGDGVRSLASEVLTDAVEPSANWSRRRIDVPRLNEDPHAAAAGAMTLLK
                     LDRLDAMIAEARHALFVTEQEGGDIRALQEELLSLQQLRRQIEDRQFLT"
BASE COUNT          609 a         1606 c         1567 g          699 t
ORIGIN      
        1 gaacgccgag gcgggcggga cgatctgggc cgacgtgcag gcgcaggtcc cgggcgcggc
       61 cgtccactcg agcggccgcg gcggcaccgt gcacgaggtc cggatcgcgg agggctcgca
      121 cctgcgcacc atcctgggcc gcgacacggc gcaggtgaac acgcaccaca tccaggccgt
      181 gaaggagatc ggcgacggct tccgcgtgag cgccgtcagc ccggacggcg tcgtcgaggg
      241 catcgaaacc gcggacgcga gcgtgatcgg cgtgcagttc cacccggagc ggcagatgga
      301 cgacctgctg ccgctcttcg tcgacctcgt gcggcgggcc gcgcagggca gcgcgacgta
      361 gtccgcgcag gggtcacggc acgtccgtcg cgacctcggg ctcggcccgc tgccaggcgc
      421 tgccgtgggg cgtcccggcg cggacagcgg gcaccgaaag cccccggccg cgttcgccgc
      481 accgtaccct cgagacgccc gtgttcgccg agcttcgatc actcgatacc ggaccccgca
      541 cgctccgccg gttcggcctc gccgtgggcg gggtgctgtt cgccgcggga gcctggttcg
      601 cgtggcgatc cggcacgccc gcggcgccgg ttccggccat cctcctcgcg gccggatccc
      661 tgctcgtcct ttcggggagc gtggctccga ccgcgctccg gtggccgttc ctcgcctgga
      721 tgggtctcgc gctcgtgctc ggagccgtga tgacccgcgt gatcctgacg ctggtctact
      781 tcctcgtcgt gaccccggtc gggctggcga tgcgggccct ggggcgggat cccatgcacc
      841 ggcgaccgga cccgggtgcg gatacgtact ggatccgccg cgaccctgac gacgatcccc
      901 gcgagcggct cacccgctac tggtgaggcc gctcaggcga tgtcccagcc gcgctcgccg
      961 cgcttcgccg tggcgaggtc catcaggatc tcctggacgc cgcgctgatc gtcgccctct
     1021 cccggccagc gcaggccgta caccccgttc gggtccagct cccacgccga gcgccggtcc
     1081 cggagcgcga tctcgagcga ccggatcagg cgggcgcgca gggcgggatc gtcgatctcg
     1141 acggtggctt cgacacggtc gtccaggttt ctccgctgcc agtctccgct cccgatcagc
     1201 acccgcggac ggtcgttgtt gcggaagtag tagatgcggg agtgctccag gaaacgcccc
     1261 aggatcgaga tcacgcggat gttctcgctg aagcgcggca gccccgggcg cagtcgcgag
     1321 tggccgcgga cgatgagatc gatctggaca cccgcctccg aggcgcggta cagctcgcgg
     1381 atcatcacgg gatcgtcgag ggcgttcatc ttggcgatca cccgcccgtt tccggtcgcc
     1441 ttctggtggc cgatctcggc gtgcagcatc tcgacgaagc ggtcccgcat gtcgcgaggc
     1501 gccacgatca ggcgccggta gtgctgctcc ggcgcatagc cggtcaggta gtggaacagg
     1561 ttgatgatgt cgaagccgac gtcgcgcgcg ctggtgaaga gccccaggtc cgtgtacagc
     1621 cgcgccgtct tcgggttgta gttcccggtc ccgatgtggc agtaggccct gaggccgtcc
     1681 gcctcctcgc ggatgaccag ggtggtcttc gtgtgggttt tcaggcccac cagaccgtac
     1741 gtcacgtgca cgccggcctt ttcgagcatc tgcccccact cgatgttgtt catctcgtcg
     1801 aaccgggcct tgacctccac gagcacggcc acctgctttc cccgctccgc cgcccggatc
     1861 agcgcatcca cgatcggaga ctcgtccgac gtccggtaca gcgtcagctt gatggcgatg
     1921 acggccgggt cctcggccgc ctcctcgacg aatcggagca cgctcgccgc gaacgactcg
     1981 tacggatgat gcaccaggag gtcgccggcg cggatcgacg tgaagatgtc ctcccggtcg
     2041 gccgcctcgc cgacgtgccg gagccgctcg ggcacgacgg gctcccacgg ctcgtagcgc
     2101 aggtccgggc gatccaggtc cgcgatcgcg tgcaggtcgc tcaggtcgat ctcgcccggg
     2161 gcctcgtaca catcgtgctc cttcagtccc agctcgcgca tgagcagcgt ccgcacgtcc
     2221 ggcggcatgc cggcctccac ctcgagccgg acgatcgggg cgaaccggcg ctcgcgcagc
     2281 tcgtcggcga tcatctcgat caggtcgtcg gcctcctcct cgtcccgccg gatgtcggcg
     2341 ttgcgcgtga tgcggaaggc gtgcacgctc gtcaccttca tgcccctgaa caggtcgtcg
     2401 atgttgttcg cgatcacctg ctcgaccggg acgaagtcga gccctcccgg caggcggatc
     2461 catcgcccgc gggccgtcgg caccttgaga cgggcgaagt gctcggtacc ccgcttcggg
     2521 tgccgcatcg tgaccgcgag cgagaggctc aggttcgaaa tgaacgggaa cgggtgtccc
     2581 gggtccaccg ccagcggcgt caggatcggg aagatgtgcg cgatgaactg ctcgtgcagg
     2641 taggccttct gctggtcgtc cagatcggca tggtccagga tccggatgcc gttcgcggcc
     2701 agcgcgggac ggagcacgcg cagccacacc tccgagaccc tgcggaccat cggctggacg
     2761 gcctgctggc acagggcgat ctgctcggcc ggcgtccggc cgtccgggct gaggcgagcg
     2821 acgccggccg cggcctggcg cttcaggccg cccatgcgct tgcggaagaa ttcgtccagg
     2881 ttcgacgccg tgatggccag gaaccggacc cgctcgagca cggggttgcg ctcgtcgagg
     2941 gcctggtaca ggacccgcca gttgaaatcg agccaggaaa gctcccggtt gaagtacagc
     3001 cgcggatccg agaggctcgc ccgcttcggc accggcagcg gcgagaccgc gcgcggcacc
     3061 acgacccgca ccgctccggc gacggccgca ggagcgggct cgccgttggt ggcttcgggc
     3121 tggatcccga cgtccggtcg ggtagtgggc gtgctcatgt caggaactgg cggtcttcga
     3181 tctgccgtcg cagctgctga agcgagagca gctcctcctg cagcgcacga atgtcccccc
     3241 cctcctgttc cgtcacgaag agcgcgtgcc gggcctccgc gatcatcgcg tcgagccggt
     3301 cgagcttcag gagcgtcatg gccccggccg cggcggcgtg cggatcctcg ttcaggcgcg
     3361 gcacgtcgat gcgccggcgg ctccagttgg ccgacggctc cacggcgtcg gtcaggacct
     3421 cggaggcgag ggaccggacc ccgtctccga acgtgccgtc caggaacgga cggcggtcga
     3481 gccgcccctc ctggtacagg cgcaccaggt ggcttaccgt ctcgcgcgag ggcccctccg
     3541 tgaactcgtc cagcgaggtc ctggaaagca cgaaagcaac catttcggac ccgttttcga
     3601 acatcagtcg caggagggtc ttctcctccg gcagcggctc tcccggcgcg ggtcgcccgg
     3661 tctcggcgga cgctcccgcg cccgccggcg cggaatcgcg ggggcgctcg ggcccgcggt
     3721 ccgaggcggc cggtcgcctc tcgccctgcc tgcgctcccg ctccagggcc cgccggaggt
     3781 acacgtccgg cacgtccagg acggacgcgg cctgctgcat gtaggactcg cgcagcagcg
     3841 ggtccgcgat gcggccgatg gtccggacca ccgcatgctg ggcggccgcg cgcgattccg
     3901 gggcgtcgag ggtcccttcg agccgggccc gctcgtgcag gtaggccacg aactcctgcc
     3961 ggtgcccggc caggtaggcc tcgaacgcgt cgcccccctg gttccggacg aaggtgtccg
     4021 ggtcctcccc gtccggaagc gtcacgacgt agggaacgag accgccctcc aggcacaggt
     4081 ctatgccccg ggccgtggcc ttcgccccgg cgtcgtcggc gtcgtacagc aggaggacgc
     4141 ggcgggcgta gcgcgagacg agccgcacct ggtcgggcgt gagcgccgtg ccgctcgagg
     4201 cgacgacgtg cccgaccccc gcctggtgca gggcgatcac gtcggtgtaa ccctcgacga
     4261 ggatcgcctc ttccttcgcg cggatcgcct gcttggcctg gtacaggccg tagagcacgc
     4321 ggctcttgtg gtagacggcc gtctcgggcg agttcacgta cttcggctgc gccgggtctt
     4381 ccaccaggat ccgtccgccg aatccgagga ccttgcccac gtgcgacagg atcgggaaga
     4441 tcagccgggc ccggtagcgg tcgtaccacc cgccgccttc c
//
LOCUS       DBNHQZ010000318         3728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2124923, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000318 DBNHQZ010000000
VERSION     DBNHQZ010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2124923"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="AC16YU_08505"
     CDS             <1..846
                     /locus_tag="AC16YU_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065305.1"
                     /translation="ERIVEGDAVVGQDGFAQYTNKAGWVGTLQVMEPGEAYALYLTGG
                     GELLYPEAPDEVAPLPAEAYETTIRGPEWRVRPERFDASMILVGEIVHSGEPLDHSAL
                     KVAVMAGDEVRGTGEIRWVEALGRFLTFVQVYGDEEPMDDLRVHVYDGGSDVLYEDVG
                     SVRYAPLTMLGTAASPVALDLANAGGAPELLDVPMEFALYPNYPNPFNPVTVIAYDLP
                     LQGKVSLTVYDVIGRRVAMLVNQEQTAGRHKVLFDGRQLASGLYLYRMEVGDFVKVGK
                     MMLVK"
     gene            complement(1000..>3728)
                     /locus_tag="AC16YU_08510"
     CDS             complement(1000..>3728)
                     /locus_tag="AC16YU_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR6065306.1"
                     /translation="VSIDAASAADFDADGWQLVRLPHDWAIAGPFDEAADGSQGKLPW
                     QGEGWYRKSIDLEAADEGKRVYLDFDGVMAFPKVYVNGALAGEWDYGYTPFRVDATDH
                     VRWGGTNVIVVHVDTGRWGSRWYPGAGIYRKVTLTLAEPVHLAEWGIRVWTNGDELAG
                     TPANTAFVETTLENHLGEPQFVTLETRLDAPDGSLLSSLPRVVRVPARSRTTVRQEFR
                     VENPLLWDVEHPHQYTLQTIVSGNDGPLDERSTRFGFRTFAFTADDGFHLNGRRVQMH
                     GVNLHHDLGPLGGAFNRAAAERQLRIMKEMGVNTLRTSHNPPAREVVELCDEMGILVW
                     DEVFDKWDWTAGRPDLEPPLPGFGHRHIRNTVLRDRNSPSVMLWSIGNELGGNEDEDG
                     IEPVRMAMMSGFVRAYDLTRPVTMAQHVPGLVDGRTFVSLDVNGWNYAHRYDRNREVL
                     PVQPLIYSESASALSTRGYYEPELPMRPTDYPPTHQVSSYDLSAASWSDIVDHEFRLM
                     ERDSFIAGEIVWTGFDYLGEPTPFTREARSSYFGIVDLCGFPKDRYFLYRSHWLPEEA
                     TVHVLPHWNWPERVGRNVPVFVYTNGDAAELFLNGRSLGLRRKGEVPERSPDLAAGAG
                     GVTASSGSESAVLAVDSDAGTGWRAAPGDSSAWWQVDLGRTETIGFVMLDLPRRENVY
                     AYVLSASADGERWTELVTKPTARFPMWGGPSRAYHEVATQARFLRVSFTEAGERSPIG
                     LDAFSVHREKVENDYYDVTYDYRLRWNEVAYEPGELRAVAFRGGEPIGEARVETVGEP
                     VALRLEAERMDLRADGDDLAFVTVMAVDAEGREHPLADDLVRFTVSGPGSIAGTCSGN
                     PLSYEPFQADHRHLFYGRALLVVRPDEGQGGEIAIVAEAEGLAPARLTIGAR"
BASE COUNT          562 a         1301 c         1297 g          568 t
ORIGIN      
        1 gagaggatcg tcgaggggga cgcggtcgtc ggtcaggacg gcttcgccca gtacacgaac
       61 aaggcgggct gggtcgggac actccaggtc atggagcccg gcgaggcgta cgccctgtac
      121 ctcacgggcg gcggagagct gctctacccg gaggcgccgg acgaggtcgc gccgctgcct
      181 gccgaggcct acgagacgac gatccggggg ccggaatgga gggtgcgacc cgagcggttc
      241 gacgcctcca tgatcctggt cggcgagatc gtgcattcgg gcgagccgct ggaccattcg
      301 gccctgaagg tcgcggtcat ggcgggtgac gaggtgcgcg gcaccggcga gatccgctgg
      361 gtcgaggcgc tgggccgctt cctgaccttc gtccaggtct acggcgacga ggagccgatg
      421 gacgacctgc gggtccacgt ctacgatggc gggtcggacg tcctgtacga ggacgtcggc
      481 tcggtccgct acgccccgct cacgatgctc ggcacggcgg cttcgcccgt cgcgctggac
      541 ctcgcgaacg cgggcggcgc acccgagctc ctggacgttc cgatggaatt cgccctctat
      601 ccgaactacc cgaacccgtt caacccggtc acggtcatag cgtacgacct gcccctgcag
      661 ggcaaggtgt cgctgaccgt gtacgacgtg atcggaaggc gggtggcgat gctcgtgaac
      721 caggagcaga cggccggacg gcacaaggtg ctctttgacg ggcgccagct cgcgagcggc
      781 ctgtacctgt accggatgga ggtcggcgac ttcgtgaagg tcgggaagat gatgctggtc
      841 aagtaggacc ggccgattcg gacccggacg gtatacggaa gcggaggtcc cggcagcggg
      901 gcctccgctt tcgcttgcct ggaagggcca tccggccacg ggagttgacg gactcccccg
      961 cccggcgcca ggcggccgct ggagtgctga cctgaacggc taccgtgccc cgatcgtgag
     1021 ccgggccggc gcgagtccct ccgcctcggc gacgatggcg atctcgccgc cctggccttc
     1081 gtcgggccgg acgacgagaa gcgcccttcc gtagaacagg tgccggtgat cggcctggaa
     1141 gggctcgtac gacaggggat ttccgctgca cgtgccggcg atcgagcccg ggccgctcac
     1201 ggtgaaccgc acgaggtcgt ccgccagcgg atgctcccgg ccctccgcgt ccaccgccat
     1261 tacggtgacg aaggccaggt cgtccccgtc ggcccggaga tccatgcgct cggcctcgag
     1321 gcgcagggcg accggctcgc cgaccgtttc cacgcgcgcc tccccgatcg gctctccgcc
     1381 ccggaacgcc accgcccgca gctcgcccgg ctcgtacgcg acctcgttcc agcgcaggcg
     1441 gtagtcgtac gtgacgtcgt agtagtcgtt ctcgaccttc tcgcgatgga ccgagaacgc
     1501 gtcgagcccg atcggcgacc gctcgcccgc ctccgtgaag gagacgcgca ggaaccgggc
     1561 ctgcgtggcg acctcgtggt acgcgcggct cgggccgccc cacatcggga acctggccgt
     1621 gggcttcgtg actagctcgg tccaccgctc cccgtcggcg gaggcgctca gcacgtacgc
     1681 gtagacattt tcccggcgcg gcaggtccag catgacgaag ccgatcgtct ccgtgcggcc
     1741 caggtccacc tgccaccacg ccgacgaatc acccggcgcg gcccgccatc ccgtcccggc
     1801 gtcggagtcg accgccagga cggcggactc cgatccgctc gatgccgtga cgcccccggc
     1861 gcccgcggcc aggtcgggcg agcgctccgg cacctcgccc ttgcgccgga ggcccaacga
     1921 gcggccgttc aggaagagct ccgccgcgtc cccgttcgtg tacacgaaga ccggaacgtt
     1981 ccggccgacg cgctccggcc agttccagtg cggcagaacg tgcacggtgg cctcctccgg
     2041 gagccagtgg ctccggtaca ggaagtagcg gtccttgggg aagccgcaca ggtccacgat
     2101 gccgaagtag gagctccggg cctcgcgcgt gaagggcgtc ggctcgccca ggtagtcgaa
     2161 gccggtccac acgatctcgc ccgcgatgaa cgagtctcgc tccataagcc ggaactcgtg
     2221 gtccacgatg tccgaccagg aggcggccga gaggtcgtag ctcgagacct ggtgcgtggg
     2281 cggataatcg gtggggcgca tcggcagctc gggctcgtag tagccgcgcg tgctcagcgc
     2341 cgacgccgac tcgctgtaga tgagcggctg gacgggcaga acctcccggt tccggtcgta
     2401 gcggtgggcg tagttccatc cgttcacgtc caggctgacg aaggtgcgtc cgtcgaccag
     2461 cccgggaacg tgctgggcca tggtcaccgg ccgggtcagg tcgtacgcgc ggacgaagcc
     2521 cgacatcatc gccatccgga cgggctcgat cccgtcctcg tcctcgttgc ctccgagctc
     2581 gttcccgata gaccacagca tgacgctcgg ggagttgcgg tcccggagca cggtgttccg
     2641 gatgtgacga tggccaaagc cgggaagggg cggctccagg tcggggcggc cggcggtcca
     2701 gtcccacttg tcgaacacct cgtcccagac caggatgccc atctcgtcgc acagctcgac
     2761 cacctcccgg gccgggggat tgtgcgacgt gcgcagggtg ttcacgccca tctccttcat
     2821 gatgcgcagc tgccgctcgg cggccgcccg gttgaaggcg ccgcccagcg ggcccagatc
     2881 gtggtgcagg ttcacgccgt gcatctgcac ccggcggccg ttcaggtgga agccgtcgtc
     2941 ggccgtgaag gcgaacgtcc ggaatccgaa gcgcgtggac cgctcgtcga gggggccgtc
     3001 attgcccgag acgatcgttt gcagcgtgta ctggtgcggg tgctcgacgt cccagagcag
     3061 cgggttctcg acccggaact cctggcgcac ggtggtccgg ctccgggcgg gaacacgtac
     3121 cacgcgcggc agggacgaaa gcagggatcc gtcgggcgcg tccaggcgcg tctccagggt
     3181 cacgaactgc ggctcgccga ggtgattctc gagcgtcgtc tcgacgaacg cggtgttcgc
     3241 cggagtcccg gcgagctcgt cgccgttcgt ccacacccgg attccccatt cggcgaggtg
     3301 cacgggctcg gccagggtga gggtgacctt ccggtagatg ccggcacccg gataccatcg
     3361 cgatccccac cgccccgtgt ccacgtgcac cacgatcacg ttcgtcccgc cccatcgcac
     3421 gtggtccgtg gcgtcgaccc ggaagggcgt gtagccgtaa tcccactcgc cggccagcgc
     3481 gccgttcacg tagaccttcg gaaaggccat caccccgtcg aagtccaggt acacccgctt
     3541 gccctcgtcc gcggcctcga ggtcgatcga cttccggtac cagccctccc cctgccacgg
     3601 caacttgccc tgggaaccgt cggcggcctc gtcgaagggc cccgcgatgg cccagtcgtg
     3661 aggtaggcgg accagctgcc agccatcggc gtcgaagtcc gcggcgctcg cggcgtcgat
     3721 cgagaccg
//
LOCUS       DBNHQZ010000319         6479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_365551, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000319 DBNHQZ010000000
VERSION     DBNHQZ010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_365551"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..2081
                     /locus_tag="AC16YU_08515"
     CDS             201..2081
                     /locus_tag="AC16YU_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR6065307.1"
                     /translation="MRLLLLTALVLAACADPRPDDPPAVPSWAADAVWYQVFPERFRN
                     GDPSNDPMRGSLPAPDRVPEGWAVTPWGSDWYARADWERAMSDDHYRTVRLRRYGGDL
                     QGVVDRLDYLERLGINALYFNPLFRARSEHKYDGATYHHVDPHFGPDPAGDLARVAGE
                     TADPATWVWTAADSLFLVMLREAHARGIRVIIDGVFNHTGRDFFAFEDLRRNQEGSRY
                     ADWYVVGAFDDPSTPGDEFDYRGWWGAASLPELADTPDGTDLHPGPKAYVFDATARWM
                     DPDADGDPSDGIDGWRLDVVPDVPIGFWADWNAHVRSLNPEAYTVAEIWEDAARSIER
                     GGFSATMNYFGFAFPVKGFLVDGRMAAGAFAAALDRSRGEHSLEVALALQNLVDSHDT
                     ERQASMIVNAGRGLPYVHEEWDVRFDYDWGARSSPAQDSLYLVRAPAEDERAVQRLVA
                     LFQMTYVGAPMVYYGTEAGMWGADDPDDRQPMVWPDIEYEDQAADPLGRERRPDPVRF
                     DSSLYDFYREAIALRRAHASLRRGSFDLVEVDEDGQGLAFSRTLEDERLVVVLNRSGR
                     EADLRLPADRLDLAPGKGLYPVFSTAGDPAAIRIAPDDEAGVTVRLPALTGAVLVRTG
                     IE"
     gene            2122..3597
                     /locus_tag="AC16YU_08520"
     CDS             2122..3597
                     /locus_tag="AC16YU_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6065308.1"
                     /translation="MLAIQRNLTKSFFALLSLPATAMGFALSVQISALSWILSTQYGL
                     DIHDVGLVWAAGPIAGILGQVIVGVISDRVWFWNGRRRPFILIGGVLATLMLLALPNI
                     DVISSAMGAGGILGVAIAVALTLDLAINVSFNPTRSVIADVTPEGVSRTKGYTWMQTI
                     SGTFGVLAYAVGAVWGNYVLIYAGAGLVLAFSIIPPFFIEEPRTLKGSSDDPGGRDEA
                     SAWAMLQAIRPLWGFLLYAVYGMTARLGNWEIEGYWVEALCLVLTVALMAESLFRSES
                     GLGREAAGALGFRKVLAAHAFTWVGVQTMFIYLFAFVQQRMPELGDVDMGRVVSISFL
                     VLNAVGALVPVLVLEPITERIGRVKTHYTSLAIMALAYAGVLFLGRTPMSIYVLMGIA
                     GIGWGAIVSLPFAIMSQKVDQGRMGLYMGLFNLSVVLPQLVASLGVGEAVSRTADKGL
                     IFVICAATLAVSALAWTLVREGGRGEVPPFSPEPLPSPPAA"
     gene            3603..5078
                     /locus_tag="AC16YU_08525"
     CDS             3603..5078
                     /locus_tag="AC16YU_08525"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIR6065309.1"
                     /translation="MRTALLLVVSLAASCAPDPVRPDLSGSPSGRGVVVHLFEWRWAD
                     VAEECETVLGPLGYSAVQVSPPSENHVVEGRPWWERYQPVSYRLETRSGSREAFADMV
                     RRCDEAGVDVIADVILNHMTDADLEHPAAGMTGTGTAGTRFEPYEYPGLYGYDDFHHC
                     GLTPNDDIEDWEDRTQLQECELVDLADLDTGSPRVRAALAAYLRDLAGLGVAGIRIDA
                     ARHVPPGDLAAILDSAGWAGYVVQEVANPRDTRLYTPLGDVTDFAYGSLVSRAVREGT
                     LDRLHGEDGIWTADPVPGDSALVFIDNHDNQRGHGVGQGVLMHRDGAAYEMSVAFMLA
                     YGRGRPRVMSSFAFETSSQGPPTSGGGETLPVHGPGGAGCGAEWVCEHRRPLVAGLVG
                     FHAAAAGAPVTLWRADAPDLVSFGRGDRAFAAFNASDTLHVFVVDTGLAPGEYCDVAQ
                     GPPAPDGRCAGRTVTVGARGEAEIPVPPMGAAALHVAAARR"
     gene            5135..5743
                     /locus_tag="AC16YU_08530"
     CDS             5135..5743
                     /locus_tag="AC16YU_08530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6065310.1"
                     /translation="MTEGRKTSNWPEPGRFWTAPNLLSMVRLVLVLPVVWLILVDGRL
                     LWIMILVVLAVATDYFDGRVARWSKTESAWGKVLDPLADKLATGLVVLALVIRGMLPT
                     WFVVLILARDLAIFGGGVLISRRTGEVPASMWVGKVAVTATAITILAALLKADPEVLR
                     FCIYATTVLLAWSFLRYMGRYLRIMARGPRRNLAVGEASDGG"
     gene            5767..>6479
                     /locus_tag="AC16YU_08535"
     CDS             5767..>6479
                     /locus_tag="AC16YU_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6065311.1"
                     /translation="MGVFDRFKAEDRREAEQASLEAGLEKTRTSLFGKLNKLVRGKDQ
                     VDEDILDELEEALVTSDVGVRTTVDIIRRVEERVARDKYVSTAGLQSLIRSEIAALLL
                     ESRPDRAADFDAPLPNRPHVIMVVGVNGVGKTTTIGKIAWHYRQAGRSVLLGAADTFR
                     AAATEQLDIWAERAGVPIIKQGHGADPAAVAFDTLAAARSRDAEVVLIDTAGRLHTKG
                     GLMDELSKVKRVMDRQVPGA"
BASE COUNT          898 a         2241 c         2349 g          991 t
ORIGIN      
        1 gtatcgcgtg acgttcgacg cgtcgcacct cgcgagcggt gcatacctgt accgcctcga
       61 gacgccaagc cgggtgatca cccgccagat gatcctggtc aagtgattcc cgggtaagat
      121 ccgatccgga acgccggggc cggtgcgcga cacgccaagc gcgccggccc cgggccgttt
      181 cggaggcacc gagcacgcgc atgcgactcc tcttactgac cgcgctcgtc ctcgcggcct
      241 gcgcggaccc gcggccggat gatccgcccg ccgtaccctc gtgggcggcc gacgcggtct
      301 ggtaccaggt gtttccggag cgcttccgga acggcgatcc gtcgaacgat cccatgcgcg
      361 ggagcctgcc cgcgcccgat cgcgtgccgg agggctgggc cgtgacgccc tggggatccg
      421 actggtacgc ccgggccgac tgggagcggg cgatgtccga cgaccattac cggaccgttc
      481 gcctccggcg ctacgggggc gacctgcagg gggtcgtcga ccgcctggac tacctggagc
      541 gcttgggcat caacgcgctc tacttcaacc cgctcttccg cgctcgctcg gagcacaagt
      601 acgacggcgc gacctatcac cacgtcgatc cgcacttcgg gccggacccg gccggggatc
      661 tcgcgcgggt cgccggcgag acggcggacc ccgcgacgtg ggtgtggacg gccgcggact
      721 cgctgttcct ggtgatgctg cgcgaggcgc acgcccgcgg catccgggtg atcatcgacg
      781 gggtcttcaa ccacacgggg cgcgacttct tcgccttcga ggacctcagg cggaaccagg
      841 agggctcccg gtatgccgac tggtacgtcg tcggcgcctt cgacgacccc tcgacgcccg
      901 gggacgagtt cgattaccgg ggctggtggg gtgccgcgag cctgcccgag ctggccgaca
      961 cgccggacgg aacggacctt cacccggggc cgaaggccta cgtgttcgac gcgacggcgc
     1021 gctggatgga ccccgacgcg gacggcgatc cctccgacgg catcgacggc tggcgcctgg
     1081 acgtggtgcc ggacgtgccg atcggcttct gggcggactg gaacgcgcac gtccgctcgc
     1141 tgaaccccga agcctacacg gtggcggaga tctgggagga cgcggcacgg tccatcgagc
     1201 ggggaggctt ctccgcgacc atgaactact tcgggttcgc cttccccgtc aagggcttcc
     1261 tcgtggacgg ccggatggcg gcaggcgcgt tcgccgcggc gctcgaccgg agccgcgggg
     1321 agcactcgct ggaagtcgcg ctcgcccttc agaacctggt cgattcccac gacaccgagc
     1381 gccaggcgtc gatgatcgtg aacgccggcc gcggcctgcc gtacgtgcac gaggagtggg
     1441 acgtccggtt cgactacgac tggggcgccc ggtcgtcgcc cgcgcaggac tcgctctacc
     1501 tcgtgcgcgc gcccgccgag gacgagcggg ccgtccagcg gctggtcgcg ctcttccaga
     1561 tgacctacgt cggggcgccc atggtgtact acggcaccga ggccgggatg tggggcgcgg
     1621 acgaccccga cgaccggcag cccatggtct ggccggacat cgagtacgag gaccaggccg
     1681 ccgacccgct gggccgggag cgccggccgg acccggtcag gttcgactcg tcgctctacg
     1741 acttctaccg ggaggcgatc gctctgcgcc gcgcgcacgc atcgctccgg cgcggctcgt
     1801 tcgacctcgt cgaggtggac gaagacgggc agggcctcgc cttctcgcgc accctcgagg
     1861 acgagcgcct ggtcgtcgtc ctgaaccgct ccggccgcga ggccgacctg cgcctgcccg
     1921 cggaccggct ggacctcgca cccggcaaag gactctaccc ggtcttctcc acggccggcg
     1981 accccgccgc gatccggatc gccccggacg acgaagcagg ggtcaccgtg cgccttccgg
     2041 ccctcaccgg tgcggtgctc gtccgcaccg gcatcgaatg atccaccctg acgggccccg
     2101 tggcccagga caccactcct catgcttgcc atccagcgca acctgacgaa gtcctttttc
     2161 gcgctcctga gccttccggc gaccgcgatg ggcttcgccc tgtcggtaca gatctcggcc
     2221 ctgagctgga tcctgtcgac gcagtacggg ctcgacatcc acgacgtggg cctcgtctgg
     2281 gcggccggcc cgatcgccgg catcctgggc caggtgatcg tgggcgtgat ctccgacagg
     2341 gtctggttct ggaacggccg gcgccggccg ttcatcctga tcggcggcgt gctcgcgacg
     2401 ctcatgctgc tggccctgcc gaacatcgac gtcatctcgt cggccatggg cgcgggcggc
     2461 atcctgggcg tggccatcgc ggtcgcgctc acgctggacc tcgcgatcaa cgtcagcttc
     2521 aaccccaccc gctcggtcat cgcggacgtg acgcccgagg gcgtgagccg cacgaagggc
     2581 tacacgtgga tgcagacgat ctcgggtacg ttcggagtgc tggcctacgc ggtcggggcg
     2641 gtctggggca actacgtgct gatctacgcc ggcgcggggc tcgtgctcgc cttctcgatc
     2701 atcccgccct tcttcatcga ggagccccgc acgctgaagg gctcgtcgga cgatccgggc
     2761 ggccgcgacg aagcttccgc gtgggccatg ctccaggcga tccgcccgct ctggggattc
     2821 ctgctctacg cggtctacgg catgacggcc cgcctgggca actgggagat cgaggggtac
     2881 tgggtcgagg cgctgtgcct ggtcctcacc gtcgccctga tggcggaatc gctgttccgg
     2941 tcggagagcg gcctcgggcg cgaggcggcc ggggcgctcg gcttccggaa ggtgctcgct
     3001 gcccacgcgt tcacgtgggt cggcgtgcag acgatgttca tctacctctt cgccttcgtg
     3061 cagcagcgga tgccggaact gggcgacgtc gacatgggcc gggtagtctc gataagcttc
     3121 ctggtcctga acgccgtcgg ggcgctcgtg cccgtactgg tgctcgagcc gatcaccgag
     3181 cggatcggca gggtcaagac gcactacacg agcctcgcga tcatggcgct ggcctacgcg
     3241 ggcgtgctgt tcctgggccg gacgccgatg tcgatctacg tgctgatggg gatcgccgga
     3301 atcgggtggg gcgccatcgt gagcctcccg ttcgcgatca tgtcgcagaa ggtggaccag
     3361 gggcgcatgg gactctacat ggggctcttc aacctgtcgg tcgtcctgcc gcagctcgtc
     3421 gcgagcctcg gcgtggggga ggccgtgagc cggaccgcgg acaaggggct catcttcgtg
     3481 atctgcgccg ccacgctcgc cgtctcggca ctcgcgtgga ccctggtgcg cgagggcggc
     3541 cggggagagg tgccgccctt cagccccgag cccctgccct cgccacccgc cgcctgaccg
     3601 ccatgcgcac cgctctgctt ctcgtcgtct cgctggccgc ttcctgcgca ccggatcccg
     3661 tccgtccgga cctgtcgggg tccccgtccg ggcggggcgt cgtggttcac ctgttcgaat
     3721 ggcggtgggc ggacgtcgcc gaggagtgcg agacggtgct cgggccgctg ggctactcgg
     3781 ccgtgcaggt gtcaccgccg tccgaaaacc acgtcgtcga gggacgtccc tggtgggaac
     3841 gctaccagcc ggtcagctac cggctcgaga cgcggagcgg cagccgggag gccttcgccg
     3901 acatggtccg gcgctgcgac gaggccggcg tcgacgtcat cgcggacgtc atcctgaacc
     3961 acatgaccga cgcggacctc gaacatccgg cggccgggat gacgggcacg gggacggccg
     4021 gcacgcggtt cgagccgtac gagtaccccg ggctgtacgg gtacgacgac ttccaccact
     4081 gcgggctgac cccgaatgac gacatcgagg actgggagga ccgcacgcaa ctgcaggagt
     4141 gcgagctggt ggacctcgcg gacctcgaca ccgggtcgcc gcgcgtgcgg gccgcgctcg
     4201 ccgcgtacct gcgggatctc gcggggctcg gcgtggccgg catccggatc gacgccgcgc
     4261 ggcatgtgcc gcccggcgac ctcgcggcga tcctggactc cgccggttgg gccgggtacg
     4321 tggtacagga ggtggccaat ccccgggata cgcgtcttta cacgcccctc ggtgacgtga
     4381 ccgacttcgc gtacggctcg ctcgtgagcc gcgcggtccg cgaaggcacg ctcgaccggc
     4441 ttcacggcga ggacggcatc tggacggccg atccggtgcc tggggacagc gcgctcgtct
     4501 tcatcgacaa ccacgacaac cagcgcggac acggcgtggg gcagggcgtg ctcatgcaca
     4561 gggacggggc cgcgtacgag atgagcgtcg cgttcatgct cgcgtacggc cggggacgcc
     4621 cgcgcgtcat gtcgagcttc gccttcgaga cctcgagcca ggggccgccg acgtcgggcg
     4681 ggggggagac gcttcccgtg cacggaccgg ggggtgcggg ctgcggcgcg gagtgggtct
     4741 gcgagcaccg gcgcccgctg gtcgccggcc tcgtgggctt ccacgcggcg gccgccgggg
     4801 cgcccgtaac gctgtggcgt gcggacgcgc ccgacctcgt gagcttcggg cgcggcgacc
     4861 gggcgttcgc ggccttcaac gcgtcggaca cgctgcacgt gttcgtcgtg gacaccgggc
     4921 tcgcgcccgg cgagtactgc gacgtggccc aggggccgcc ggccccggac ggacgctgcg
     4981 cggggcggac cgtgacggtc ggcgcccgcg gcgaggcgga gatcccggtg ccgccgatgg
     5041 gcgcggcggc cctgcacgtg gccgccgctc gcaggtgatc ccgctttcgg ccgcggcggg
     5101 cgccgtatct tctcaccacc caccccccga ggcgatgacc gaaggcagga agacgtccaa
     5161 ctggccggag ccgggtcgtt tctggacggc accaaacctg ctgtccatgg tccggctcgt
     5221 gctggtttta cccgtggtgt ggctgatcct ggtggacggg aggctgctct ggatcatgat
     5281 cctcgtggtg ctcgccgtcg ccacggacta cttcgacggc cgggtcgccc gatggtcgaa
     5341 gaccgagtcc gcctggggaa aggtgctcga cccgctcgcg gacaagctgg ctacgggcct
     5401 cgtggtgctc gcgctggtca tccgggggat gctcccgacg tggttcgtgg ttctgatcct
     5461 cgcccgcgac ctggccatct tcggcggagg cgtcctgatc agccggcgca cgggcgaggt
     5521 cccggcgagc atgtgggtgg gaaaggtggc cgtgacggcc acggccatca cgatcctggc
     5581 cgcgctgctg aaggccgatc cggaggtcct gaggttctgc atctacgcga ccacggtgct
     5641 gctcgcctgg tccttcctgc gatacatggg ccgctacctg cggatcatgg cgcggggccc
     5701 gcgcagaaac ctggcggtcg gcgaagcgtc ggacggcggc tgacccgata gcgtgccgag
     5761 gggacgatgg gcgtattcga tcgattcaag gcggaggacc gccgcgaagc ggagcaggct
     5821 tcgctcgagg ccgggctcga aaagacccgg acgagcctgt tcggcaagct gaacaagctg
     5881 gttcgcggca aggaccaggt ggacgaggac atcctggacg agctcgagga agcgctcgtc
     5941 acgagcgacg tcggcgtcag gaccacggtg gacatcatcc ggcgcgtgga ggagcgcgtg
     6001 gcgcgcgaca agtacgtgtc cacggccggc ctgcagtcgc tgatccggag cgagatcgcg
     6061 gccctcctcc tggagagccg gcccgaccgg gcggcggact tcgacgcgcc gctcccgaac
     6121 cgcccccacg tgatcatggt cgtcggcgtg aacggggtgg gcaagacgac gacgatcggc
     6181 aagatcgcct ggcactaccg gcaggcgggg cgctcggtgc tcctcggcgc ggccgacacg
     6241 ttccgcgcgg ccgccacgga gcagctggac atctgggccg agcgggcggg cgtgccgatc
     6301 atcaagcagg ggcacggggc cgatcccgcg gccgtcgcct tcgacacgct cgcggcggcc
     6361 aggagccggg acgccgaggt cgtcctcatc gatacggcgg gacgcctgca cacgaaaggc
     6421 gggctcatgg acgagctgtc caaggtgaag cgggtcatgg accggcaggt gcctggagc
//
LOCUS       DBNHQZ010000320         9733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3072769, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000320 DBNHQZ010000000
VERSION     DBNHQZ010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3072769"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="AC16YU_08540"
     CDS             <1..935
                     /locus_tag="AC16YU_08540"
                     /inference="COORDINATES: protein motif:HMM:NF037850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HIR6065312.1"
                     /translation="GTRGEYSLGDDPDRPAPFYTRSMVGAEQLWKFVSLNDWFAGDFM
                     WTGVDYLGESRWPRKSSTSGVIDLVGFPDDGWWFYRSRWTTEPMLHLFPHWNWEGREG
                     QFIPVIAYTNVDAVELYLNGRLVGEKVIAFPRQGTSGCWNCYASPPVFPTTADLHMTW
                     DVPYEAGTLRAVGKRAGEVVVTAEIHTAGPAAALRLSADPESILSNVRDVSHVRVEVV
                     DAAGRVVPTADVPVTFEISGAGRLLAVGNGNPADHDSFQSNRRTTFNGLALAIVQSGP
                     DDGTIRFSARADGLEGASIEIRVVPGLAPRTVPH"
     gene            1097..3604
                     /locus_tag="AC16YU_08545"
     CDS             1097..3604
                     /locus_tag="AC16YU_08545"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR6065313.1"
                     /translation="MLRGLLILAAASVVASCGQAPRPAVLDKEEILARQSFWDNRDFD
                     WYAANVPFFESPEPLIDRTWWYRWEVVTKHLTYGSPETGYTFTEFIDRPFWSGTYGGI
                     SCPLGHQHYEVRWLKDRRIVEDFARYWFETPGAEPRSYSNWYADAMWATYLVTGDRDF
                     LRTVFPHMEAQYAGWIEERWDEGHRMFRWDGMHDGMETNINSRLTADEFSGAEGYRPT
                     LNSYLVADARALSKAAALLGDDAKAADYAARASDLRQRILDELWDPGREFFFHQYARD
                     EKDGIRAKSLTYETGPHAGSPYGREEIGFVPWQFHVPTEGYEGAWRFLADTARFAAPF
                     GPTTVERNDPQFFVSPTCCVWSGNQWPYATTQTLAALANLLIDYEQDVVTKDDYVDLL
                     LTYARLHDKDGSPYVAEAADPFTGSWEGHDTFYHSEHYFHSGFTDLVVTGLVGLRPRE
                     DDVISVRPLAPDAWDWFALDDVRYRGRTLSILWDRDGTRYGRGAGLSILVDGERLASR
                     PDLGPLDVPFEPGPPDRAERPANFAVNNDGSHFPFASASWSGPATPPFWAQDGSRWYH
                     DSPPNHWSSAGSPNSVDWFEVDFGAARPLDRLVLYFMENRTLKPPARFDVQVRGDSGW
                     VPVDGPRTPDVPTGRRANTVELGGIVTAGVRIVFEASPGAAVGLTEVEAWGPDDPALA
                     EHVIPAPNLALRARASASFTSPNDAVEQVNDGRIALSRYSRNRWTAWNSPTRSDWVML
                     EFDAPETVSAAELFLYDDGRGVRTPEAYRIEARSDGRWVPVSETGRLPADPLGFAVNT
                     VRFEPVTTDAVRITFVHPVPAWSGASEIRLWSDLPTN"
     gene            3610..5169
                     /locus_tag="AC16YU_08550"
     CDS             3610..5169
                     /locus_tag="AC16YU_08550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6065314.1"
                     /translation="MIPGMRGREHQLVLLVVAWTAVKVALHLLVPEGWDLHRDEYLYL
                     ALGRHLAWGYWSTGPFVGAISWLVQAYSDAGPIDVRLPAIVASALTLAVTAWMTHRMG
                     GRTVAISLASLAMLGSPAFLRSGSMFQPVVFDVLWWTVAAACVVGYLTSGRRGWLVAL
                     GPVVGLGLLTKYSMGLFAVSLLVAALLTRHRRAVFTRWTASAAVIAALVVLPNLLWQA
                     RYGYPVFTHLAGLATTHLVHVRATGFLLDQVLMFLPAIVVWVGGLVWLFGSGGRHFRI
                     LGWLWLACLLLVLGMSGKSYYVMGAYPALMAAGGVRFERFRSRWPARVAVVFVIATSV
                     ALAPYSVPVIAPAPMERYADTVAQWTGIDEARRWEDGTYHALPQDYADMQGWRTLADR
                     VDLAVEAAPDSGSTVVYAENYGQAGAIEYYGRSTERVVSFSDSYRLWAPDRLPPDLRA
                     FVYVNSELGADIDSLFGIVVPMGGITESTARERGTSVWLCLNPHPGFYDHYAHRVAPV
                     KQGLPRLPGSP"
     gene            complement(5166..5435)
                     /locus_tag="AC16YU_08555"
     CDS             complement(5166..5435)
                     /locus_tag="AC16YU_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065315.1"
                     /translation="MYDLIGKRVRVHLYGRDGMIVGAVTGRVADFAPRVQVSDGMVKD
                     LVYVVDIATGEPEVPYRNSAGGENESWFAVQDVEVIDEGPRLFAN"
     gene            complement(5440..5820)
                     /locus_tag="AC16YU_08560"
     CDS             complement(5440..5820)
                     /locus_tag="AC16YU_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065316.1"
                     /translation="MDPGHSGIRPGRPRRPLAFDDLVADSAVVKGVARDDVQPGDRVV
                     VYTHNSVYVLRRIDEHRFEVSGGWFDRQGPDPAVLSVTGCTWGGSAVRRDLVAGCGMR
                     IEFGNRVLTSTVRRVVHYPGVMLN"
     gene            complement(5869..6132)
                     /locus_tag="AC16YU_08565"
     CDS             complement(5869..6132)
                     /locus_tag="AC16YU_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065317.1"
                     /translation="MGLVEWILLFLAALILPPTFQAIFARREDVPRTRRPPSSRGPAM
                     RMADDRQPGSHERTAPGENSHAADAGAGARPGPSPLHGKEPSA"
     gene            complement(6231..6812)
                     /gene="hisH"
                     /locus_tag="AC16YU_08570"
     CDS             complement(6231..6812)
                     /gene="hisH"
                     /locus_tag="AC16YU_08570"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR6065318.1"
                     /translation="MNVAVVRYNAGNIQSVRYALGRLGIEPDVTDDPEMLRAADRVIF
                     PGVGEASSAMRYLAGRGLDAVISGLTQPVLGICLGLQLLCTHSEEADTRCLGIFPETV
                     RRFPPGAKIPHIGWNTITGLRGPLFDRVADGSHVYFVHGFHADLGDDTTARSDYIVPF
                     SAGLSRGNFHAVQFHPEKSGETGARILANFLAL"
     gene            complement(6809..7408)
                     /gene="hisB"
                     /locus_tag="AC16YU_08575"
     CDS             complement(6809..7408)
                     /gene="hisB"
                     /locus_tag="AC16YU_08575"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR6065319.1"
                     /translation="MIGREVERPHRAAEVTRRTNETDIRVRVDLDGTGRTRIRTGLGF
                     FDHMLEQIGRHGGIDLDVTVRGDLHVDEHHTVEDTALALGACLRQALGDKRGIERYGF
                     LLPMDEALAQVALDLSGRAYLVWDVPLSRERVGDVPTELFEHFFRSFCDAAACTLNVR
                     AAGENEHHVIESVFKGVARALGTAVRRGAGGVPSTKGSL"
     gene            complement(7394..8680)
                     /gene="hisD"
                     /locus_tag="AC16YU_08580"
                     /pseudo
     CDS             complement(7394..8680)
                     /gene="hisD"
                     /locus_tag="AC16YU_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
     gene            complement(8686..9537)
                     /gene="hisG"
                     /locus_tag="AC16YU_08585"
     CDS             complement(8686..9537)
                     /gene="hisG"
                     /locus_tag="AC16YU_08585"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6065320.1"
                     /translation="MLRLALQKSGRLAEQSVELVRDCGIRFSRADGKLRAVADNFPVE
                     LLYLRDDDIPGYVADGVAHAGIVGQNVVAESGRSVDVAERLGFGRCRLSIAVPRAGSI
                     EDLSSLQGAHVATSYPRVLAGFLESKGIQATIHEISGSVEIAPGIGLADAICDLVSTG
                     STLISNGLRELATVMPSEAVLIANPSLDAEASALLDALRFRVGSVLRARGFRYILLNV
                     PDRALEEVKAILPGMKSPTVMPLAETGWSSVHSVIRDDEFWEIIDRLKAVGAEGMLVV
                     PIEKMVQ"
BASE COUNT         1418 a         3410 c         3414 g         1491 t
ORIGIN      
        1 gcggcacccg cggcgagtat tccctcggcg acgatcccga tcgcccggct ccgttctata
       61 cccgcagcat ggtgggcgcc gagcagctct ggaagttcgt ctcgctgaac gactggttcg
      121 ccggggactt catgtggacg ggcgtcgact atctcggcga gtcacgctgg ccgcggaaga
      181 gctcgacctc gggcgtcatc gacctggtcg gcttcccgga cgacggctgg tggttctacc
      241 ggagccggtg gaccacggag ccgatgctgc acctgtttcc gcactggaac tgggaggggc
      301 gcgaggggca gttcatcccg gtcatcgcct acacgaacgt cgacgccgtc gaactgtacc
      361 tgaacggccg gctcgtcggc gagaaggtga tcgcgtttcc ccggcagggc acgtcgggct
      421 gctggaactg ctacgcctcg ccgccggtct ttcccacgac ggccgacctg cacatgacct
      481 gggacgtgcc gtacgaggcc ggcacgctcc gggcggtcgg aaagagggcc ggcgaggtcg
      541 tcgtgacggc ggagatccac accgccggtc cggcggcggc gctgcgcctg tccgcggacc
      601 ccgaatcgat cctgtcgaac gtgcgcgacg tctcgcacgt tcgggtggag gtcgtcgacg
      661 ccgcggggcg cgtcgtgccg accgcggacg tgcccgtgac gttcgagatc tccggagcag
      721 gccgtctcct cgccgtcgga aacggcaatc cggccgacca cgactccttc cagtcgaacc
      781 gccgtaccac cttcaacggt ctcgccctcg cgatcgtcca gtccggtccg gacgacggga
      841 cgatccggtt ctcggcacgc gccgacggac tggagggtgc ttcgatcgag atccgcgtcg
      901 tgcctgggct cgccccgcgg acggtgcccc attgaggacc cggcgtgggc ctgcctccgt
      961 gcccggcggg acgattgctc cggacacggg cgggcgcgcg gattccgaat aatcggaacg
     1021 gatacgttgt atgcagatga gaggtacgca ccctgtggtg taggttatgg ctattggcgt
     1081 acctcccgaa ccgatcatgc tccgaggcct cctgattctg gcggccgcgt ccgtcgtggc
     1141 atcctgcgga caggcgcccc gccccgccgt cctggacaag gaggagatcc tcgcgcgcca
     1201 gtccttctgg gacaaccggg acttcgactg gtacgcggcg aacgtgccct tcttcgagtc
     1261 gccggagccg ctgatcgatc gcacgtggtg gtaccggtgg gaagtcgtga cgaagcacct
     1321 cacgtacggc tcgccggaga cggggtacac gttcacggag ttcatcgacc gcccgttctg
     1381 gtccggcacg tacggaggaa tcagctgccc gctcgggcac cagcactacg aggtccgctg
     1441 gctcaaggac cggcgcatcg tcgaggactt cgcccggtac tggttcgaga cgccgggcgc
     1501 cgagccgcgc agctactcga actggtacgc ggacgcgatg tgggccacgt acctggtcac
     1561 gggggaccgg gatttcctgc ggaccgtctt cccgcacatg gaggcgcagt acgcgggctg
     1621 gatcgaggag cgctgggatg aggggcaccg catgttccgg tgggacggca tgcacgacgg
     1681 gatggagacg aacatcaaca gccgtctcac ggccgacgag ttctcggggg cggagggcta
     1741 ccggcccacg ctcaactcct acctggtggc ggacgcccgc gccctgtcga aggcggcggc
     1801 gctgctgggc gacgacgcga aggccgccga ttacgccgcg cgcgcgtccg acctccggca
     1861 gcggatcctg gacgagctct gggaccccgg gcgggagttc ttcttccacc agtacgcccg
     1921 cgacgagaag gacggcatcc gggcgaagag cctgacgtac gagaccgggc cgcacgccgg
     1981 gagtccgtac ggccgcgagg agatcggctt cgtgccgtgg cagttccacg tgccgaccga
     2041 aggctacgaa ggggcctggc gcttcctggc ggacacggcc cgtttcgcgg cgcccttcgg
     2101 tccgacgacc gtcgagcgga acgacccgca gttcttcgtt tccccgacgt gttgcgtctg
     2161 gagcggcaac cagtggccgt acgcgaccac gcagacgctc gcggcgctcg cgaacctgct
     2221 gatcgactac gaacaggacg tggtcacgaa ggacgactac gtggacctcc tcctcaccta
     2281 cgcacggctg cacgacaagg acggcagccc ctacgtcgcc gaggcggcgg accccttcac
     2341 cgggtcgtgg gagggacacg acaccttcta ccacagcgag cattacttcc actcggggtt
     2401 cacggacctc gtcgtcacgg ggctcgtggg cctccggccg cgggaggacg acgtgatctc
     2461 ggtgcgtccg ctcgctcccg acgcctggga ctggttcgcg ctcgacgacg tgcgctaccg
     2521 gggccgcacg ttatccatcc tgtgggaccg ggacggcacg cgctacgggc gcggcgcggg
     2581 tctctcgatc ctggtggacg gggagcgcct cgcgtcgcgg ccggacctgg ggccgctgga
     2641 tgtgccgttc gagccgggtc ccccggaccg ggcggagcgt ccggcgaact ttgccgtgaa
     2701 caacgacgga tcccacttcc ccttcgcgag cgcctcctgg agcggcccgg ccacgccgcc
     2761 cttctgggcg caggacggct cccgctggta ccacgattcc ccgccgaacc actggagctc
     2821 ggcgggctcc ccgaactccg tcgactggtt cgaggtcgat ttcggcgctg cgcgtcccct
     2881 ggaccggctc gtgctctact tcatggagaa ccgcaccctg aagccgcccg cccggttcga
     2941 cgtccaggtc cggggcgact cgggctgggt gcctgtcgac ggaccgcgca cgccggatgt
     3001 cccgaccggg cgccgggcga acacggtgga gctcggcggc atcgtgaccg cgggcgtgcg
     3061 gatcgtattc gaagcctcgc cgggcgcagc cgtcggcctt acggaagtgg aagcctgggg
     3121 tccggatgac ccggcgctcg cggagcacgt gattccggcg ccgaacctgg ccctgcgggc
     3181 gcgggcgtcc gcatccttca cctcgccgaa cgatgcggtc gaacaggtga acgatggccg
     3241 gatcgccctc tcgcgctact cccgcaaccg gtggaccgcg tggaattcgc cgacccggtc
     3301 ggactgggtg atgctcgagt tcgacgcccc cgagacggtc tccgccgccg aactcttcct
     3361 gtacgacgac gggcgcggcg tccgcacgcc ggaggcctac cgcatcgaag cccggtcgga
     3421 tggccgctgg gtgcccgtct cggagaccgg ccgcctcccc gcggaccccc ttgggttcgc
     3481 cgtgaacacg gtccggttcg agccggtcac gaccgacgcg gtccgcatca cgttcgttca
     3541 ccccgtgccg gcctggtccg gcgcaagcga aatcaggctg tggtccgacc tgccgacgaa
     3601 ctgagatccg tgatcccggg catgcggggg agggagcacc agctcgtgct cctcgtcgtc
     3661 gcgtggacgg ccgtgaaggt cgccctgcac ctgctcgtgc ccgagggctg ggacctgcac
     3721 cgggacgagt acctgtacct ggcgctcggc cggcacctgg cctggggcta ctggtccacc
     3781 ggcccgttcg tcggcgcaat ttcctggctc gtgcaggcat attccgacgc cggtccgatc
     3841 gacgtccggc tgcccgcgat cgtcgcgagc gccctgacgc tcgccgtaac ggcctggatg
     3901 acgcaccgca tgggcggcag gacggtcgcc atctcactcg catccctcgc catgctgggg
     3961 tcgccggcct tcctgcgctc cggatcgatg ttccagcccg tggtgttcga cgtgctgtgg
     4021 tggacggtcg cggcggcctg cgtcgtgggc tacctcacgt ccggtcgccg cggctggctc
     4081 gtggccctcg gcccggtcgt gggtctgggc ctgctgacca agtattcgat ggggctgttc
     4141 gcggtgtcgc tcctcgtcgc ggcgctgctc acgcgccacc gtcgcgcggt gttcacccgc
     4201 tggaccgcgt cggccgcggt gatcgctgcg ctcgtcgtgc tgcccaacct gctgtggcag
     4261 gcccggtacg gctacccggt cttcacgcat ctcgcggggc ttgccacgac ccatctggtc
     4321 cacgtgcgcg caaccgggtt cctcctggac caggtgctca tgttccttcc ggccatcgtc
     4381 gtgtgggtgg gcgggcttgt ctggctgttc ggttcgggcg gccggcactt caggattctc
     4441 ggatggctgt ggctggcgtg cctgctgctc gtgctgggca tgagcggcaa gtcgtactac
     4501 gtgatgggag cgtacccggc cctcatggcg gcgggcggcg tccggttcga gcggttccgc
     4561 agtcgctggc cggcccgtgt agcggtggtg ttcgtcatcg cgacaagcgt ggcgctcgcg
     4621 ccgtactcgg tccccgtgat cgcgccggcg cccatggagc ggtatgccga tacggtcgcc
     4681 cagtggaccg ggatcgacga ggcccggcgc tgggaggacg gcacgtacca tgcgctgccc
     4741 caggattatg ccgacatgca gggctggcgg acactggccg accgggtgga tctggccgta
     4801 gaggcggcgc ccgacagcgg atcgacggtc gtctacgccg agaactacgg gcaggccggt
     4861 gcgatcgagt actacggccg gtcgaccgag cgcgtcgtaa gcttcagcga cagctaccgc
     4921 ctgtgggcgc ccgaccgcct tccgcctgac cttcgcgcgt tcgtgtacgt gaactcggag
     4981 ctgggtgcgg acatcgacag cctcttcggc atcgttgtac cgatgggcgg aatcaccgag
     5041 tcgaccgcgc gggagcgggg cacgagcgtg tggctgtgtc tgaacccgca tccgggattc
     5101 tacgatcact acgcccaccg cgtggctccc gtgaagcagg gccttccgag actcccaggc
     5161 agcccctagt tcgcgaacag ccggggcccc tcgtcgatca cttcgacgtc ctgcaccgcg
     5221 aaccaggact cgttctctcc gccggccgaa ttccggtacg gcacctcggg ctcgcccgtg
     5281 gcgatgtcca cgacgtagac caggtccttg accatcccgt ccgaaacctg caccctcgga
     5341 gcgaaatccg cgacccgccc cgtcacggcg cccacgatca tgccgtcgcg gccgtacagg
     5401 tggaccctca cgcgctttcc gatcagatcg tacatcggat cagttcagca tgacgcccgg
     5461 atagtggacc acccgacgca cggtggacgt cagtacccgg ttgccgaact cgatccgcat
     5521 gccgcatccg gcgacgagat cccgccggac ggccgagccg ccccacgtgc atccggtgac
     5581 cgacaggacg gcggggtccg gtccctgccg gtcgaaccag ccccccgaga cctcgaacct
     5641 gtgctcgtcg atgcgacgca gcacatagac cgagttgtgc gtgtacacca ccacgcggtc
     5701 tcccggctgg acgtcgtccc gcgccacgcc cttcacgacc gccgagtcgg cgacgagatc
     5761 gtcgaaggcg agcggccgac gagggcggcc ggggcgaatg cctgaatgtc cgggatccac
     5821 gcgaagtcgc ttcgttggtg agggtggggt gcccgacggc ccccgacgtt acgcactcgg
     5881 ctccttccca tgcaacgggg aaggcccggg ccgcgcgccg gccccggcgt ccgccgcatg
     5941 cgaattctcg cctggtgcgg tccgctcatg ggacccgggc tgccggtcgt cggccatccg
     6001 catggccggg ccgcgcgagg agggcggcct gcgggtgcgc gggacgtcct ccctccgggc
     6061 gaaaatcgcc tggaaggtgg gcggcaggat gagggccgcc aggaagagca ggatccactc
     6121 gaccaggccc atggacgtcg gggtgctacg gacaggagaa gcatcggcgc aatgccgagc
     6181 cgactcaaac ggggcactcc gggatgcccg gaatgcggtt cattaaacgc ctacagagcc
     6241 agaaagttgg cgagtatccg ggcgccggtc tcgccgctct tctcgggatg gaactgcacg
     6301 gcatggaagt ttccacggct cagaccggcc gaaaacggca cgatgtaatc ggacctcgcg
     6361 gtggtatcgt cgccgaggtc ggcatggaag ccgtgcacga aatacacgtg agatccgtcg
     6421 gccaccctat cgaacagcgg cccacgaagt ccggtgatcg tgttccaccc gatgtgcgga
     6481 atcttcgcgc ccggcggaaa tcggcgcacg gtctccggga atattccgag gcagcgcgtg
     6541 tccgcctctt ccgagtgggt gcacagcagc tgcagtccca ggcagatgcc cagcacgggc
     6601 tgcgtgagcc ccgagatgac cgcatccagc ccccgcccgg ccaggtatcg catcgcggag
     6661 gaggcctcgc cgacccccgg gaagatgacg cggtcggccg cccggagcat ctccgggtcg
     6721 tccgtgacgt ccggttcgat ccccaggcgg cccagcgcgt agcgcaccga ctggatgttg
     6781 cccgcgttgt atcggacgac cgcgacgttc acagggaccc cttggtgctc gggacgcccc
     6841 cggctccccg ccgcacggcc gttccgaggg cgcgcgcgac gcccttgaat acggactcga
     6901 tcacgtggtg ctcgttctcg cccgcggcgc gcacgttcag cgtgcatgcg gccgcgtcgc
     6961 agaacgagcg gaagaagtgc tcgaagagct ccgtcggcac gtctcccacg cgctcccgcg
     7021 agagcggaac gtcccacacc aggtaggccc ggcccgagag gtcgagggcc acctgggcga
     7081 gcgcctcgtc catcgggagc aggaagccgt agcgctcgat gccccgcttg tcgccgaggg
     7141 cctgccgcag gcacgcgccg agcgccaggg ccgtgtcctc gaccgtgtgg tgctcgtcca
     7201 cgtgcaggtc gccccggacc gtcacgtcca ggtcgatgcc gccgtgacgg ccgatctgct
     7261 cgagcatgtg gtcgaagaag ccgagacccg tccggatccg ggtgcggccc gtgccgtcca
     7321 ggtccacgcg cacgcggatg tcggtctcgt tcgtgcggcg cgtgacctcg gccgcccggt
     7381 gcgggcgttc gacctcccgt ccgatcacgt cgaggcgcgc ggagaccgaa cgggcgtgcc
     7441 cctcgagcga ttccgcccgc gccatggtct cgacggccgg acccaggcgg cgcaggccgt
     7501 ccgcggtgag cgcctggaac gtgatcttcc ggacgaagga atccagcgac acccccgacc
     7561 agccgcgtgc cgcgccgccc gtaggcagcg tgtggttggt gccggacgcg tagtctcccg
     7621 cggcctcggg cgcgaggggg ccgacgaaga ccgatcccgc gtgctccacc cgctcggccg
     7681 ccgcctccgc gtcgtccacg gcgaggacca ggtgttccgg agcgtaggcg ttcacgaggg
     7741 cgatcgcctc gtccatgtcc ccgacgacga cgcagcggct gccctcgagc gcacgccgcg
     7801 cgatgtccga gcgggggagt tccgcgagct gccgccttag ttcgctctcg acgcgcccgg
     7861 gaagagaggc atccgtggtg acgaggagga cctggctgtc cgggccgtgt tcggcctgcg
     7921 ccaggaggtc ggcggcgacg aagcgcgccg gggccgtcgc gtcggcgagc accgcgagct
     7981 ccgtcggacc cgccggcagg tccacggcca cgccggcgag cgaggcgagc tgcttcgcgg
     8041 cggtcacgta ctggttgccg gggccgaaca gcttgtctac ccgcggcacg gactcggtgc
     8101 cccacgccat ggccgcgatc gcctgggcgc cgccgatgcg gaacacgcgg tcgatgccga
     8161 gctcggacgc ggcgaagagt atggccgggt gcagccgccc gtcgggcccc ggcggggagc
     8221 agagcacgat ttcggggcaa ccggcgagcc tggccgggac gccgagcatc ggcacggtcg
     8281 agaagagcgg agccgtgccc cccggcacgt acaggcccac gctccggatc ggaacgctcc
     8341 gacgccagca cgtgacgccc ggcgtcgtct cgacgcgggc gcctgcgtcg cgctgcgtgg
     8401 cgtggaaccg ctccaggttg cggatcgcga tccggatcgc gtcctgcagc tcgggtgcga
     8461 cgtggtcggc ggccgcccgg atctcgtccg cggggacggc cggatcgtcg acgaggacgc
     8521 cgtccagctc ctgcgtgaag cggcgcacgg ccgcgtctcc gccggtccgg acggcctcca
     8581 cgatcgggcg gacctgctcc acggtcgcct cgagcgaccg cgtcgggcga accacgagcc
     8641 cgggccagtc ggcgcgcggc ggacggtcga tgacgcgcag caggctcatt gcaccatctt
     8701 ctcgatgggt acgacgagca tgccctcggc gcccacggcc ttcaggcggt cgatgatctc
     8761 ccagaactcg tcatcgcgga tcaccgagtg caccgagctc cagccagtct ctgcgagcgg
     8821 catgaccgtg gggctcttca ttccgggcag gatcgccttg acctcttcga gcgcccggtc
     8881 gggcacgttc aggaggatgt agcggaagcc gcgggcccgg agcaccgatc ccacgcggaa
     8941 gcggagggcg tcgagcagcg ccgaggcctc ggcatccagg gaggggttcg cgatgagcac
     9001 cgcctcggac ggcatcacgg tggcgagctc gcgcagcccg ttcgagatga gcgtcgagcc
     9061 cgtcgagacc aggtcgcaga tggcgtcggc gagcccgatg cccggcgcga tctcgaccga
     9121 accgctgatt tcgtggatgg tcgcctggat gcccttcgat tccaggaaac ccgccaggac
     9181 gcgcgggtag gaggtggcga cgtgggcgcc ctggagcgag gacaggtcct cgatcgagcc
     9241 cgcccggggc acggcgatcg acagccggca ccggccgaaa cccagtcgtt ccgcgacgtc
     9301 gaccgaccgg ccggactcgg ccacgacatt ctgccccacg atgccggcgt gcgccacgcc
     9361 gtcggccacg tacccgggga tgtcgtcgtc gcgcaggtac agaagctcga ccgggaaatt
     9421 gtccgcgacc gcgcgcagct tcccgtcggc cctggaaaag cggatcccgc agtcgcggac
     9481 cagctccacg gactgctcgg cgaggcggcc ggacttctgg agggcgaggc ggagcatggg
     9541 ctcgtcgggt ggcgaatgtg catgtactcg cggccgtacg ggcggttcta tgcgggtccc
     9601 gttgatcccg gccttccggg cggctaacct gcacgcgaac cgccgcgctc ccatggacac
     9661 ccgattcgca acacacctgg cctggacgag cgacgcgccg ctcggcctgt ccgtggaccg
     9721 ggcggacggc ccg
//
LOCUS       DBNHQZ010000321         6778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1584276, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000321 DBNHQZ010000000
VERSION     DBNHQZ010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1584276"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC16YU_08590"
     CDS             <1..473
                     /locus_tag="AC16YU_08590"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065321.1"
                     /translation="AGLRYLEERPGDDEGAAETAGAGRRFAAALGGQDASGTPGEAIP
                     GAVPDAQALGALTHRDARSALDLADAAYAADSEGPLAAVALAGALARAGREDEALSLL
                     AAGLESDPVTPLVLMTAGDLLYSLGRREEARAAWQRGLDASPESPVFRSRLAGR"
     gene            473..1237
                     /locus_tag="AC16YU_08595"
     CDS             473..1237
                     /locus_tag="AC16YU_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025490.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HIR6065322.1"
                     /translation="MTRTIRLGLLLVAVGSLASCRATRPPTAPLPAGFPHHSAPEVLA
                     LLPSGDSLWSTFRIEAALAFATPDGSGSFSAHILVRRPDSVLVRIRAPLGIEVARALV
                     TPDSVLLYDRVERTLYAGPSDADLLPAGLHASDVSAAFFGFDRPPGGDWTIRPDSLLY
                     RLERGDDSETLLVDPRWWRITARDTRDATGTVTERRRFTEFTRYGNLVVPRRIVTLRP
                     VEGTRASFFVRKVEPYPTDTDMDLGVRSDARRIAIR"
     gene            1242..2432
                     /locus_tag="AC16YU_08600"
     CDS             1242..2432
                     /locus_tag="AC16YU_08600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6065323.1"
                     /translation="MFRRTSILVLVFLALGSVRALAQTEDMKRTEEQLNALKAQIQSD
                     EQQLERTRSAERATLRQLEQINRDIRLREELVATYARRVGELDVEVDSVFRQIEATSE
                     HVESLRQDYAERVRAAYKHGRMNTVALILAANSVNQMLVRVNYLRRYRDVRVRKRDQL
                     EEATGQLERRRERLTEILAQTQEMRAEAISENQRLADLRRDRTRVVTDLRNRSTEIEQ
                     ELTAKRDAERNLQGRLTELREAAARSRAAQGDGAVDAAFRALTGAFADNRGRLPWPAD
                     GTLIQRFGWTADPVYGTRVNNRMIFVATPAGVDIRAVFEGVVQDVDIMATYGRYVMVA
                     HGDYWTVYGNLSSTSMQPGTVVSAGDVLGQTGSTATDTGSGYFFGLFRENDFTDPLPW
                     LMPR"
     gene            2532..3164
                     /gene="rpiB"
                     /locus_tag="AC16YU_08605"
     CDS             2532..3164
                     /gene="rpiB"
                     /locus_tag="AC16YU_08605"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR6065324.1"
                     /translation="MSRRTLIRETDVLAAARAGSDTLVVPPGAVVTPLARDTARERGV
                     RLVAPDPGSGRPDAPAPRVIAVGSDHGGFALKSELIPLLRELGWAVADVGTGSTAPCD
                     YPDFAYAVARQVADGSAAFGLMIDGAGIGSAIVCNKVPGVRAAAAYNEFTAWNARAHN
                     DANVLTLGSRTLGIEVCRSLVRVFLSTAFEGGRHAARVRKIDDVDAAAGR"
     gene            3289..4710
                     /gene="lpdA"
                     /locus_tag="AC16YU_08610"
     CDS             3289..4710
                     /gene="lpdA"
                     /locus_tag="AC16YU_08610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6065325.1"
                     /translation="MASSPYDVIVIGSGPGGYETAIRASQLGMKTAIVEKNKLGGVCL
                     NIGCIPTKALLKSAELMEYAGHLDAYGLTLDGTVAPDFPKVIARSRGVADKMSKGVQF
                     LMKKNKIEVVLGTARLAGGGKVTVEPSVDMDGNEIGEKTTIAGKHIIVATGARARSLP
                     SLPVDGKHIVSYREAMLQTTPPRRLVVVGAGAIGVEFAYFYHHMGTDVTIVEIMDRVV
                     PIEDEDVSKELARAFKKAGIKVMTSSAVEEVDTSGKSLKVTVKTSKGTETIEADQVLS
                     AVGVVGNIEDLGLEALGVKTEKGAIVVDGFGSTGVEGIYAIGDVAGPPWLAHKASHEG
                     ILCVEKIAGLDVHPMDKRSVPGCTYCQPQIASVGYTEKAAKEAGYELKVGKFPFTASG
                     KASAMGHTEGFVKIIYDAKYGELLGCHIIGYDATELIAEIVAARALETTGHEIMEAIH
                     PHPTLSEAVMEASKAAYGRPMNI"
     gene            4830..5465
                     /locus_tag="AC16YU_08615"
     CDS             4830..5465
                     /locus_tag="AC16YU_08615"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6065326.1"
                     /translation="MTLRLGDTAPDFRARTTEGDISFHDWLGDGWGVLFSHPADFTPV
                     CTTELGAVARIKDEFEKRNVKVIAISVDPLESHLEWIGDINETQACTMNFPIIADSDR
                     TVATLYDMIHPNADDKATVRSVFVIGPDKKIKLTITYPASTGRNFDEILRVIDSLQLT
                     AEHRVATPANWLEGEDCIVVPALSDEDASKLFPMGFRKVKPYLRYTPQPNR"
     gene            5576..6139
                     /locus_tag="AC16YU_08620"
     CDS             5576..6139
                     /locus_tag="AC16YU_08620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6065327.1"
                     /translation="MQKPQRWNSLGAADGITLRRATVDDAGALSEAAARLFFQAYDGA
                     IAAAEMEHYVRSNFVPERQRAEIADPGCVALLALDGERPAGYAQVRRADAPDGTGCPA
                     RAQLWRIYVDRAWHGTGLGAGLLAEAAAAAREFSGTGMWLAVWEQNPRAIAFYLKHGF
                     TPVGRQAFRVGSEVQSDLVLCSPGLAG"
BASE COUNT         1065 a         2420 c         2348 g          945 t
ORIGIN      
        1 aggccggact gcgctatctc gaggaacggc cgggcgatga cgagggcgcg gcggagaccg
       61 ccggcgccgg acgccgtttc gcggccgccc tgggcgggca ggacgcatcc gggacgcccg
      121 gcgaggccat ccccggtgcc gtcccggacg cgcaggccct cggggcgctc acgcaccggg
      181 acgcccgcag cgcgctcgac ctggccgacg cagcgtacgc cgccgattcc gaggggccgc
      241 ttgccgcggt ggccctcgcc ggggcgctcg cccgcgcggg ccgcgaggac gaggcccttt
      301 ccctcctcgc cgccggcctg gaatccgacc cggtgacccc gctcgtactg atgaccgccg
      361 gggacctgct ctattccctc ggccggaggg aagaggcgcg cgccgcgtgg cagcggggcc
      421 tcgacgcctc gccggagagc cccgtttttc gcagccgcct ggcgggtcga tgatgacgcg
      481 cacgatccgt ctcggcctcc tgctcgtcgc cgtcgggagc ctcgcatcct gccgcgcgac
      541 gcgcccgccg accgcgccgc ttccggccgg tttcccgcat cactcggccc ccgaggtgct
      601 cgccctgctc ccctcgggag actcgctctg gtccacgttc cggatcgagg cggcccttgc
      661 gttcgcgacg ccggacggca gcggatcgtt ctccgcgcac atcctggtcc ggcggcccga
      721 ttccgtgctg gtccggatcc gcgcaccgct cggcatcgag gtcgcgcggg cgctcgtcac
      781 gccggattcc gtcctgctgt acgaccgggt ggaacggacc ctgtacgcgg gcccctccga
      841 cgccgatctc ctcccggccg gcctgcacgc gtccgacgtt tcggcggcct tcttcggctt
      901 cgaccgcccc cctggcggcg actggaccat ccgcccggac agcctgctgt accggctgga
      961 acgcggggac gattcggaga cgctgctcgt cgatccgcgc tggtggcgga tcacggcccg
     1021 cgacacgcgc gacgcgaccg gcaccgtgac cgagcggcgc cggttcaccg agttcacgcg
     1081 ctacggcaac ctcgtcgtac cgcgccgcat cgtcaccctg cgccccgtcg agggaacaag
     1141 ggccagcttc ttcgtacgaa aggtcgagcc gtatccgacc gataccgaca tggatctcgg
     1201 ggtccgttcc gacgcccgcc gcatcgccat ccgctgaccg catgttccgc cgcacgtcca
     1261 tcctggtcct ggtcttcctc gcgctcggct ccgtccgggc gctggcccag accgaggaca
     1321 tgaagcgcac cgaggagcag ctcaacgccc tgaaggcaca gatccagagc gacgagcagc
     1381 agctcgagcg gacgcggtcc gccgagcgcg ccaccttgcg ccagctcgag cagatcaacc
     1441 gcgacatccg cctgcgcgag gagctcgtgg cgacgtacgc ccggcgcgtg ggcgagctgg
     1501 atgtcgaggt cgacagcgtg ttccggcaga tcgaggcgac gtccgagcat gtcgaatcgc
     1561 tcaggcagga ctatgcggag cgggtgcgcg ccgcctacaa gcacgggcgc atgaacaccg
     1621 tcgcgctgat cctcgccgcg aattccgtga accagatgct ggtgcgcgtg aactacctgc
     1681 ggcgataccg ggacgtccgg gtccggaagc gggaccagct cgaggaagcc accgggcagc
     1741 tggaacggcg gcgggagcgg ctgaccgaga tcctcgccca gacgcaggag atgcgcgcgg
     1801 aggccatctc cgagaaccag cggctggccg acctgcgccg ggaccggacg cgcgtcgtca
     1861 ccgacctcag gaaccgcagc acggagatcg agcaggagct caccgcgaag cgcgatgccg
     1921 agcgcaacct gcagggccgg ctcacggagc ttcgcgaggc cgccgcccga agccgggccg
     1981 cccagggcga cggcgcggtg gacgcggcct tccgcgcgct gacgggcgcc ttcgcggaca
     2041 accgcggacg gctcccctgg cctgccgacg gcaccctcat ccagcggttc ggatggaccg
     2101 cggacccggt gtacggcacg cgggtcaaca accggatgat cttcgtcgcc actccggcgg
     2161 gcgtcgacat ccgggctgtg ttcgagggcg tggtccagga cgtggacatc atggccacgt
     2221 acggacgcta cgtgatggtc gcgcacgggg attactggac ggtgtacggc aacctttcct
     2281 cgacctccat gcagcccggc accgtggtct cggccggcga cgtgctgggg cagaccggga
     2341 gcacggcgac cgacaccggc agcgggtact tcttcggcct gttccgcgaa aacgacttca
     2401 cggatcccct gccgtggctg atgccgcggt gatggccgga tccgcgaccg ggtgagcagg
     2461 ctcgccggct gccgccgtca cggcgccgcg cgccccgtgg cgcccgacga cacccggccg
     2521 tagcttgcgg catgtcgcgc cgaacgctca tacgggagac ggacgtcctc gccgcggccc
     2581 gggcgggctc ggacacgctg gtcgtgccgc cgggcgccgt ggtgacgccc ctcgccaggg
     2641 acacggcgcg tgagcgaggc gtccgcctcg tcgcgccgga tcccggatcc ggtcgcccgg
     2701 acgcccctgc tccccgggtg atcgccgtcg gaagcgatca cggagggttc gccctcaagt
     2761 cggagctcat tcccctgctg cgtgagctcg gctgggcggt cgcggacgtc gggacgggct
     2821 cgacggcccc ctgcgactac cccgatttcg cgtacgccgt cgcgcggcag gtggccgacg
     2881 gatctgcggc cttcggactc atgatcgacg gcgcgggcat cggatcggcg atcgtgtgca
     2941 acaaggtgcc cggcgtgcgc gccgccgccg cgtacaacga gttcacggcg tggaacgccc
     3001 gggcgcacaa cgacgcgaac gtgctcacgc tcggcagccg gacgctcggc atcgaggtgt
     3061 gccggagcct ggtccgcgtg ttcctgtcga ccgcgttcga gggcggcagg catgccgccc
     3121 gcgtgcgcaa gatcgacgac gtggacgccg cggccggccg ctgaacgggc gaagtccgcg
     3181 cgtacggccc gttccacccg gtattcaccg ccgttcgctt tccagccccg ccggggacgg
     3241 gtatcttttc cgccctcccg tgcaccccga cgaccaatac ccagcgtcat ggcttcctcc
     3301 ccgtacgacg tcatcgtgat cggaagcggc cccggcggct acgagaccgc catccgcgcc
     3361 tcccagctcg gcatgaagac ggccatcgtc gagaagaaca agctcggcgg cgtctgcctg
     3421 aacatcggct gcatcccgac caaggcgctg ctcaagagcg cagagctcat ggaatacgcg
     3481 ggccacctcg acgcgtacgg cctgacgctc gacggcacgg tcgcgccgga cttcccgaag
     3541 gtgatcgcgc ggagccgggg cgtcgccgac aagatgagca agggcgtcca gttcctcatg
     3601 aagaagaaca agatcgaggt cgtcctgggc acggcccggc tggccggcgg agggaaggtc
     3661 accgtggagc cgtccgtgga catggacggc aacgagatcg gcgagaagac cacgatcgcc
     3721 ggcaagcaca tcatcgtcgc gaccggggcg cgcgcccgct cgctgccgtc gctgcccgtc
     3781 gacggaaagc acatcgtctc gtaccgggag gccatgctcc agaccacgcc gcccaggcgg
     3841 ctggtcgtgg tcggcgcggg cgccatcggg gtcgagttcg cctatttcta ccatcacatg
     3901 ggcaccgacg tcaccatcgt cgagatcatg gaccgcgtgg tcccgatcga ggacgaggac
     3961 gtctcgaagg agctcgcacg cgccttcaag aaggccggta tcaaggtcat gaccagctcg
     4021 gccgtcgaag aggtcgacac ctcgggcaag tcgctgaagg tgaccgtcaa gacgtcgaag
     4081 gggacggaga cgatcgaggc cgaccaggtg ctctcggccg tcggcgtcgt ggggaacatc
     4141 gaggacctgg ggctcgaggc gctgggcgtc aagacggaga aaggcgccat cgtcgtggac
     4201 ggcttcggaa gcaccggcgt cgagggcatc tacgcgatcg gcgacgtcgc cgggccgccg
     4261 tggctggcgc acaaggcgag ccacgagggc atcctgtgcg tcgagaagat cgccggcctc
     4321 gacgtgcacc cgatggacaa gaggagcgtc cccggctgca cctactgcca gccacagatc
     4381 gcgtcggtcg gatacaccga gaaggcggcg aaggaggccg gatacgagct gaaggtgggc
     4441 aaattcccgt tcaccgcatc gggcaaggcg tcggcgatgg gccacaccga gggattcgtg
     4501 aagatcatct acgacgcgaa gtacggcgag ctgctcggct gccacatcat cggttacgac
     4561 gcgaccgagc tcatcgccga gatcgtcgcc gcgcgggcgc tcgagaccac cggccacgag
     4621 atcatggagg cgatccaccc gcacccgacg ctctcggagg cggtcatgga ggcgtccaag
     4681 gcggcgtacg gccggccgat gaacatctga tcggccaggg gcggtgcttg accgcgaggt
     4741 gcttgctgcc cccgcgaacc ccgggcggcg tcggtacgta ctccatccga ccgtaccacc
     4801 cacgaccggc tgaacggagg aagctgccca tgaccctcag gctcggcgac acggcgcccg
     4861 atttccgtgc caggaccacc gaaggcgaca tctccttcca cgactggctc ggcgacggat
     4921 ggggcgtcct gttctcccat cccgccgatt tcacgccggt ctgcacgacc gagctcggcg
     4981 cggtggcccg gatcaaggac gagttcgaga agcgcaacgt gaaggtcatc gcgatcagcg
     5041 tcgacccgct cgaatcccac ttggagtgga tcggcgacat caacgagacc caggcgtgca
     5101 cgatgaactt cccgatcatc gccgactccg accgtacggt ggccacgctc tacgacatga
     5161 tccacccgaa cgccgacgac aaggcgaccg tgcgatctgt cttcgtgatc ggaccggaca
     5221 agaagatcaa gctgacgatc acctaccctg cgagcacggg caggaatttc gacgagatcc
     5281 tgcgcgtgat cgattcgctc cagctcacgg ccgagcacag ggtggcgacg ccggccaact
     5341 ggctcgaggg cgaggactgc atcgtcgtgc cggcgctctc ggacgaggac gcctcgaagc
     5401 tcttcccgat gggcttccgg aaggtgaagc cgtacctgcg ctacacgccc cagccgaacc
     5461 gttagagccg ccggccgatc agccggcagc gatcggtgcc gggacgtcgc cccgcggcga
     5521 gtccccgccg tcccgccgga ccagatgccg acggcgagtg gaaccgcggc caccgatgca
     5581 gaaaccgcaa cgctggaatt cgctgggcgc ggcggacggg atcaccctgc gccgggccac
     5641 cgtcgacgac gccggagcgc tgtccgaggc ggccgcgcgc ctgttcttcc aggcgtacga
     5701 cggggcgatc gcggccgccg agatggagca ctacgtccgc tcgaacttcg tgcccgaacg
     5761 gcagcgcgcg gagatcgccg accccgggtg cgtggcgctg ctggccctgg atggagaacg
     5821 ccccgcaggg tacgcccagg tccggcgggc ggacgccccg gacggcaccg gctgcccggc
     5881 ccgcgcccag ctctggcgca tctacgtgga ccgggcatgg cacgggaccg gtctcggtgc
     5941 cgggctcctc gccgaggcgg ccgccgccgc ccgtgagttc tccgggaccg gcatgtggct
     6001 cgccgtatgg gagcagaacc cgcgcgccat cgccttctac ctcaagcacg gcttcacgcc
     6061 ggtcgggcgg caagccttcc gggtgggttc cgaggtccag agtgacctgg tcctctgctc
     6121 gccaggtctc gcgggctgaa ccgctgccgc cccctctcgc cggcccggac cacgaggacc
     6181 cgcccccggc atgcgtccgg atcgacgatg gggatccggg tgattccgcg gccgcgggcg
     6241 gtgagcgtgg gcgccggccg gacacgtgcg cgcgaccgga gcgggagacc gactcatggt
     6301 gcggtcccgg cgcacctccc cgatccgtcc gacgaatcag cactctgcct acgggagaat
     6361 gtggaatggc ctgccacggc cccggagtgg accggcgtac gttcctgtac acccacagtg
     6421 cgccacgcca tgacgaaacc gagccgttga gccgtacgtc tcgccgtccc accgcagatc
     6481 agggccgatc ggattggccg tggcagcgcc cgtggtacgg acagtcacgg tgaaaccctt
     6541 tccggcgctc ggcgaaacgc gccttccgga caccgccacc cgacaggacg tcgttctcgg
     6601 aagtgctgca cgccatggac atatcggaat cgggccgcgc gggaagtgac gcggcaagtg
     6661 cccggccggc tctccgggcc ggcctgcggc gtggtcgtta ccggcgatgg acggtggtca
     6721 tcctgctgct gctgatgctg gtgctgctgg gggcgttggc gtccttcgag atccggac
//
LOCUS       DBNHQZ010000322         3664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1314906, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000322 DBNHQZ010000000
VERSION     DBNHQZ010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1314906"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="AC16YU_08625"
     CDS             <1..1038
                     /locus_tag="AC16YU_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065328.1"
                     /translation="TPEILENLEFVINASSHHPGANHFYIHAVEAVDPNRAVEQAERL
                     AGLMPGAGHIVHMPGHIYVRVGRYRDAILANEHAVHADETFIQDHNPAQGVYVGGYYP
                     HNYDFLAFAASMIGREEQSVAAGEKLRTLAPVELIHVPGMTFLQAHRTRHLQLKVRFG
                     LWQEILSVPEPEAGLLHERAIWHYARGRALDALGRAVEAAIESARLDAIATNPEVAGL
                     RLEFNSSAAILGIASAVLKAKIAHSAGDTRSAAGHLDEAARLEDALTYGEPPEWTVPV
                     RQEAGRMLLSAGMPAEAERAFLVDLERFPANGWSLFGLSEALAAQGRDAEADAARARF
                     REMWEGGGAPW"
     gene            complement(1094..2422)
                     /locus_tag="AC16YU_08630"
     CDS             complement(1094..2422)
                     /locus_tag="AC16YU_08630"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6065329.1"
                     /translation="MGLVLALVASLAAGSGCDSGGTDQRDLVDEAALDDSLVAVLTRH
                     GERRLENFMLPDDGDLASIPQDPLNPITPEKVSLGALLFHDPALATSPRHPAGAGTYS
                     CSTCHHAGAGFQAGRIQAIAEGGSGWGAGGEGRRADPSYPVEDIDAQGIRSPSVVNSA
                     YQQAMMWDGRMGSRGPNAPTSARWIPGTLEGVNLLGYEGVESQAIAALTDHRSDSVSA
                     WPVPSHPTYAALWEAAFPGQAVSRELAGLAIAAYERTLLASKAPFQRWLRGDTRAMTV
                     DEKRGALVFFAKAGCDECHSGPALNSMAFYALAMPDMPSSAMVRPLPPSLGRGGFNGV
                     DDDLFKFKVPQLYNLADSPFYGHGGTFPTLRSVVEYYNDGEPAVVLPPGRLEPRFVPL
                     ELTDEEVDQLTAFLEGALNDPDLERFVPASVPSGGCFPANEPAARSDLGC"
     gene            complement(2457..3158)
                     /locus_tag="AC16YU_08635"
     CDS             complement(2457..3158)
                     /locus_tag="AC16YU_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR6065330.1"
                     /translation="MSSRRFRDPSAWFAALILPAVLLGCDSQPGLTEEADPPSVPVLA
                     SPADGADDVADDLALEWSPADDGTVFHVIVATDRDLTEVRAEVPNHPSPLFVLTNLQI
                     GKTYFWRVRAVNPAGQSAWSPVRSFTPARAGVPPPSPDLVAPVHDAITPLETVLYWNA
                     APGAVSYDVQVAQEDVFVRMDVDREGILTTYRNIGNLITGYEYFWRVRARNYSGVSAW
                     SPSRRFHTSFQTPGS"
     gene            complement(3413..>3664)
                     /locus_tag="AC16YU_08640"
     CDS             complement(3413..>3664)
                     /locus_tag="AC16YU_08640"
                     /inference="COORDINATES: protein motif:HMM:NF023367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6065331.1"
                     /translation="LRRSRADLQRGGFVMLGTGNPAVLAFARSLDDRETAVILNLSDH
                     AATLDLASIGVSGHDVVLGTHPDGRSLALEPWAFSILVR"
BASE COUNT          566 a         1251 c         1350 g          497 t
ORIGIN      
        1 acgcctgaga ttctggagaa cctcgagttc gtcattaacg ccagctcgca ccacccggga
       61 gcgaaccatt tttacatcca tgccgtcgag gccgtcgatc cgaaccgggc ggtcgagcag
      121 gccgagcgcc tggcgggcct gatgccgggt gccgggcata tcgtccacat gcccggacac
      181 atctacgtcc gggtggggcg gtaccgggac gcgatcctcg cgaacgagca cgccgtgcac
      241 gcggacgaga ccttcatcca ggaccacaac ccggcccagg gggtctacgt ggggggatac
      301 tacccgcaca actacgactt cctggccttc gcggccagca tgatcgggcg cgaggagcag
      361 tcggtggctg cgggggagaa gctgcgcacc ctcgctcccg tcgagctcat ccacgtgccg
      421 ggcatgacct tcctccaggc gcaccggacg cgccaccttc agctgaaggt ccgattcggc
      481 ctgtggcagg agatcctgtc cgttccggag cccgaggcgg gcctcctgca cgaacgggcg
      541 atctggcact acgcgagggg ccgggcgctc gacgccctgg gccgggccgt cgaagcggcc
      601 atcgaatccg cgaggctcga cgccatcgcc acgaacccgg aggtggccgg gctccgcctg
      661 gagttcaact cgtcggccgc gatcctcggc atcgcgtccg cggtgctgaa ggcgaagatc
      721 gcgcactcgg ccggtgacac ccggtccgcg gcgggccacc tcgacgaggc cgcccggctc
      781 gaggatgccc tgacctatgg cgagccgccc gagtggaccg tgcccgtccg ccaggaggcc
      841 ggtcggatgc tgctctcggc cggcatgccg gccgaggcgg agcgggcctt cctggtggac
      901 ctggagcgct tccccgcgaa cggctggtcg ctcttcgggc tgtccgaggc gctcgcggcg
      961 cagggcaggg acgccgaggc ggacgccgcg cgcgcgcgct tccgggagat gtgggaaggg
     1021 ggcggcgcgc cctggtgatc attcggggag cccggttccg cgctgcgcca ccaggggcgc
     1081 agcggggccg ggctcagcac ccgaggtccg agcgggcggc gggctcgttg gccgggaagc
     1141 atccccccga gggaaccgat gccggcacga aacgctccag gtccggatcg ttgagcgcgc
     1201 cctcgaggaa ggccgtgagc tggtccactt cctcgtcggt gagttccagg ggcacgaaac
     1261 ggggctcgag gcggccgggc ggcaggacga cggccggctc gccgtcgttg tagtattcga
     1321 ccacggagcg aagcgtcggg aacgtgccgc cgtggccgta gaagggcgag tccgccaggt
     1381 tgtagagctg cggtaccttg aacttgaaaa ggtcgtcgtc cactccgttg aaccccccgc
     1441 gcccgagaga ggggggcagg ggccgcacca tggcggagga cggcatgtcc ggcatggcca
     1501 gggcgtagaa ggccatcgag ttcagcgccg gccccgagtg gcactcgtcg cagccggcct
     1561 ttgcgaagaa cacgagcgcg ccgcgcttct cgtctaccgt catggcccgc gtgtcgcccc
     1621 gcagccaccg ctggaaaggg gccttcgatg cgaggagtgt ccgctcgtag gcggcgatgg
     1681 cgaggcccgc gagctcgcgc gagaccgcct ggcccgggaa ggccgcctcc cagagggcgg
     1741 cgtacgtggg atgagagggg acgggccagg cggagaccga gtccgagcgg tggtccgtga
     1801 gcgcggcgat ggcctgcgac tcgacgccct cgtagccgag caggtttacg ccctcgagcg
     1861 ttccgggaat ccaccgggcc gacgtcgggg cgttgggtcc cctggacccc atgcgcccgt
     1921 cccacatcat cgcctgctgg tacgccgagt tcacgaccga cggcgaccgg atgccctggg
     1981 cgtcgatgtc ctcgaccggg tacgacggat cggcacggcg cccctcgccg cccgctcccc
     2041 agcccgatcc gccctcggca atcgcctgga tccggccggc ctggaacccg gctcccgcgt
     2101 ggtggcacgt ggagcacgag taggtcccgg cacccgcggg atgccgcggc gacgtcgcga
     2161 gcgccgggtc gtggaagagg agcgcgccga gcgacacctt ctccggcgtg atcgggttga
     2221 gcggatcctg ggggatcgag gccaggtccc cgtcgtccgg gagcatgaag ttctcgagac
     2281 ggcgctcgcc gtggcgggtc agcacggcga cgagggagtc gtcgagggcg gcctcgtcca
     2341 cgaggtcacg ctgatccgtg ccgcccgagt cgcatccgga tccggccgcg agggacgcga
     2401 cgagcgcgag gaccaggccc acgcgcctgg cgaggcctcc ggccgcggcg gcgcccctag
     2461 cttccgggag tctggaaact ggtgtggaac cggcgcgaag gcgaccaggc cgacacgccg
     2521 gagtagttgc gagcccggac gcgccagaaa tattcatacc cggtgatcag gttgccgatg
     2581 tttcgatacg tggtcaggat gccctcccgg tccacgtcca tccgcacgaa gacgtcttcc
     2641 tgcgccacct gcacgtcgta cgaaacggcc cccggggccg cgttccagta caggacggtc
     2701 tcgagcgggg tgatggcgtc gtggaccggg gcgaccaggt ccggcgaggg gggcgggacg
     2761 cctgcccggg ccggcgtgaa cgagcgcacg ggcgaccagg cgctctgccc ggccgggttc
     2821 accgcgcgga cgcgccagaa gtaggtcttc ccgatctgca ggttcgtcag cacgaagagc
     2881 ggcgagggat ggttcggaac ctccgcccgc acctccgtga gatcgcggtc cgtggccacg
     2941 atgacgtgga atacggtccc gtcatccgcg ggagaccatt ccagcgccag gtcgtcggcg
     3001 acgtcgtcgg cgccgtccgc gggcgaggcg agcaccggca ccgacggagg gtccgcttcc
     3061 tcggtgaggc cgggctgcga atcgcagccc aggaggaccg ccggcaggat cagcgcggca
     3121 aaccaggccg acgggtcgcg gaacctgcgg gacgacatga gggggattcc tccacgaagc
     3181 atgcagacgg acacggaaaa tggcatgttc cgtccaacct cggcgttcgg cccaggattc
     3241 gtgtctcccg gattttgccc gacgaatggc gtccagtctc gtagggaaat gcccggcacg
     3301 gggtaatcgg gaggcccgcc cggggtgccc ggcttcgccc ggagggcggc gcccgacgac
     3361 gtggaccgcg cacccacgcg ctcgccggcg atccccgcca ggccttgacg gatcaccgca
     3421 cgaggatgga gaaggcccag ggctcgagcg ccaggcttcg tccgtcggga tgcgttccga
     3481 gcaccacgtc gtgccccgag acgccgatgg acgccaggtc cagggtcgcc gcatggtccg
     3541 acaggttcag gataaccgcg gtctcgcggt cgtcgaggct ccgggcaaat gccaggacgg
     3601 ccggattgcc cgtgccgagc atgacgaagc cgccccgctg caggtcggcg cggctccgac
     3661 ggag
//
LOCUS       DBNHQZ010000323         6188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3480710, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000323 DBNHQZ010000000
VERSION     DBNHQZ010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3480710"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..945
                     /locus_tag="AC16YU_08645"
     CDS             91..945
                     /locus_tag="AC16YU_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR6065332.1"
                     /translation="MRIALVQQHAGPDPDANRQRGLDALERAARAGAELVVWPELAFT
                     RFFPQHRLSGDRFAHSETVPGPTTDAFSEAARRLGVVVVLNLFERAGAEAYDASPVID
                     ADGSLLGVTRMMHITQYEGFWEQDYYTPGDTGAPVYRTAAGRIGVAICYDRHYPETMR
                     AFALQEADLVVVPQAGAVGEWPEGLLEAEMRVAAFQNGYFTALANRVGREDVLTFAGG
                     SFVTDPFGRVTVRAPEGEEALLVADLDLSCLADAPARKLFLEHRRPQEYREGAVLIGD
                     RRGADVRA"
     gene            complement(1000..1545)
                     /locus_tag="AC16YU_08650"
     CDS             complement(1000..1545)
                     /locus_tag="AC16YU_08650"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6065333.1"
                     /translation="MRPSFRTYRRLALVLVVAGLGFVPASAQPAAGDPAPTAPTWIPF
                     EEALLAADSSGKMVLVDVWSARCGWCARMQREVYSRPDLVAYLNEHFETGRLDIDVRT
                     DTIAYMGYELSSAELSMALGASGTPTTVFLGPDGTYVTRLPGFHPYERFLKVLRFIGS
                     DAYREMTFEEYEESPGSSGGG"
     gene            complement(1554..2738)
                     /locus_tag="AC16YU_08655"
     CDS             complement(1554..2738)
                     /locus_tag="AC16YU_08655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6065334.1"
                     /translation="MNLESTVTARHPSDVAFAMTGDVHRNTRALRQLDALCGAGLTVT
                     LLAFPPTTLRTTLPHGVDPVYVPEPGRRGPRRFHSIHRAFTRALRGVDAAVFHASDLY
                     VLRAVRSAAARTRAPFTYDARELYAHVAATAGRPLVSRYWRAFEATPARQAAVVWTVS
                     DAIADHLARTYGITRPTVLLNAPVAGPVRPDPYLRSATGLEADTPLFVHLGQVRPGRG
                     GEALVRAFARVPGVALAFVGYGPGMPALERLASDLGTAGRIFFLDPVPPGRIRPVIAA
                     ADVGVTLLEDSCLNHRYALPNKLFDYLAAGLPVLASRLPELGRVVEGHDVGLTVDPGD
                     PAALADAVRRMGTGATQRERWARNALAAAETFDWSKASERFTSPIRSLVTAASGGQRS
                     VP"
     gene            2849..3886
                     /locus_tag="AC16YU_08660"
     CDS             2849..3886
                     /locus_tag="AC16YU_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6065335.1"
                     /translation="MGVTIYDIAREAGVSIATVSRVVNGHERVAEATREQVLAVALEL
                     GYQPHASARSLARRRSNVIAAVIPMLTNYFFIEVLRGVQDRMAESDFDLLVFSARTLD
                     EVGDQLVRALAKGRSAGVLLFSSPMDPERTRQIIASGRPVTLVDETHEAFDAVSTDNL
                     LGGRLAVRHLLDRGARRIALIMARPESIPAADRHEGYRIALGEYGIECDPALVVVSED
                     GLYHGYSEAAGYEAMQRLLRARGPAPDAVFATSDVQALGAMRALQEAGIRVPEDMLVV
                     GFDDIMISRHLGLSTVRQPMYEMGRLGADKLFARIENPDHEVSHTILAPRLVVRRSSG
                     VPSTAPTRVAS"
     gene            3883..5550
                     /locus_tag="AC16YU_08665"
     CDS             3883..5550
                     /locus_tag="AC16YU_08665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6065336.1"
                     /translation="MSRPASIASLLVLGAVLAACGGSGERILAEVGDRVVTQDGFRDA
                     YVDYLVSTGLPDDPARRTSFLERLVSMELVRLDALEHGLGEGDAWRDAEERARRRLLL
                     DAWIAEVAFDTLTVDQAELEDLFVRVNTEITARHLYAPTREEAERLADRLAAGETFED
                     LAREVFADPALAASGGSLGTFGFDEMDPAFEEVAFTLEPGSVSGPVRTSQGWSILRVD
                     DRFTRPLLTETEFAARMPQLRAYVLERERKLARREVLEEMLISTAPEIEPSVADRVLG
                     LLGPAVVGAEDVNADAVLIRYTDDGESRAWTVGQFLAAARYTDLEQRRAVRDRSTFEA
                     FATGLLVRDVIMERSRQAGLDRSARFREALDSAMDEWLYERGYERIAESEPVPEDTLR
                     AFFDRHAAELVLPERVAVREIVTTGRAEADSIRAALSGASFAELARRHSVRSATSGAG
                     GDLGLLSRADLGPLADRVFAAPRGAVIGPIPVPGQYLLLEVGETSESRPATFDEAREQ
                     IEAIVRREQRDRLVRERAALLRDRFAVRYADVDLAALPLRSEPVVSS"
     gene            5564..>6188
                     /locus_tag="AC16YU_08670"
     CDS             5564..>6188
                     /locus_tag="AC16YU_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6065337.1"
                     /translation="MRKIWSATLLLAVLTAGSAAAQTGKISGRVLDAATGESLPGVNV
                     VIEGTMQGTATNLDGEYVIIGVRPGTYDVLASFIGYSTERKEGVRVNLDLTTTVDFDL
                     QEEVIMGEEIVVTAEAVGVRKDVTSSEARVTSETLDRLPVTELAQVLEVQAGITNRGG
                     LHIRGGRSSEVMFMVDGVPVTDSYDGSTSVQLENDGIEELQVISGTFN"
BASE COUNT          900 a         2139 c         2253 g          896 t
ORIGIN      
        1 ccgatcttcc tcctgaaacg ccggccctgc cggccattac gcgccggccc tgaaacgccg
       61 gcccccgaag taccagacct gccgagcgcc atgcgcatcg cactcgtaca gcagcacgcg
      121 ggccccgacc cggacgcgaa tcggcagagg ggtctcgacg ccctggagag ggctgcccgg
      181 gccggcgccg agctggtggt ctggccggag cttgcgttca cgcggttctt cccgcagcac
      241 cgcctgtcgg gcgatcggtt cgcacattcg gagaccgtgc cgggtccgac gacggatgcc
      301 ttctcggagg ccgcccgccg cctgggcgtg gtcgtggtgc tgaacctgtt cgagcgcgcg
      361 ggcgcggagg cgtacgacgc ctctcccgtg atcgacgccg acgggagcct gctcggcgtc
      421 acccggatga tgcacatcac ccagtacgag ggcttctggg agcaggacta ctacacgccc
      481 ggggacacgg gggcgccggt ctaccgtacg gcggcgggcc ggatcggggt cgcgatctgc
      541 tacgaccggc actacccgga aacgatgcgc gcgttcgccc tgcaggaggc ggacctcgtg
      601 gtcgttcccc aggccggcgc cgtgggtgag tggccggagg ggctcctgga ggccgaaatg
      661 cgtgtcgccg cgttccagaa cggctatttc accgccctgg ccaaccgggt gggccgggag
      721 gacgtgctga ccttcgccgg cggctccttc gtgacggatc cgttcggacg cgtgaccgtc
      781 cgggccccgg agggcgagga ggcgctgctg gtcgccgacc tggacctgtc gtgcctcgcg
      841 gacgccccgg cgcgaaagct cttcctggag caccgaaggc cgcaggagta ccgcgaggga
      901 gcggtcctca tcggggaccg gcgaggtgcg gacgtgcgcg cctgacgatc gggtcggccg
      961 cgcccaggga ggaggaagcc gcgcgccggg gatgccgcct cagccgcccc cggaggatcc
     1021 cgggctctcc tcgtactcct cgaacgtcat ctcccggtag gcgtccgagc cgatgaaccg
     1081 gaggaccttc aggaagcgct cgtacgggtg gaatcccgga agccgggtga cgtacgtgcc
     1141 gtcggggcca aggaagaccg tcgtgggcgt tcccgacgcg ccgagcgcca tcgagagctc
     1201 ggccgacgac agctcgtatc ccatgtaggc gatcgtgtcc gttcggacgt cgatgtccag
     1261 gcggcccgtc tcgaagtgct cattcaggta ggccacgaga tccggccggg agtagacctc
     1321 ccgctgcatg cgggcgcacc atccgcaccg tgccgaccag acgtcgacga gcaccatctt
     1381 tccggaggaa tcggccgcca ggagcgcctc ctcgaacggt atccaggtcg gcgcggtcgg
     1441 cgccggatcc cccgccgccg gctgggcgga ggccggcacg aagccgaggc cggccaccac
     1501 gaggacgagc gcaagacgtc ggtatgtacg gaaggacggg cgcattcggt gcttcagggc
     1561 acgcttcgct ggccgccgga cgcggccgtg accaggctcc ggatcgggga cgtgaagcgc
     1621 tcagatgcct ttgaccagtc gaatgtttct gccgcggcca gggcattccg ggcccagcgt
     1681 tcccgttgcg tggcgcccgt gcccatccgg cggacggcgt cggcgagggc cgcggggtcg
     1741 cccgggtcca ccgtgaggcc gacgtcgtgg ccctcgacga cgcggccgag ctccggcagc
     1801 cggctggcca gcacgggcag gccggcggcc aggtagtcga agagcttgtt cggcagggcg
     1861 tagcggtgat tcaggcagga atcctcgagc agcgtcacgc cgacgtccgc cgccgcgatg
     1921 accggccgga tccgtccggg cgggacggga tccaggaaga agatccgccc ggcggtgccg
     1981 agatccgagg cgagccgctc gagggccggc atcccgggcc catagccgac gaaggccagg
     2041 gcgacgcccg gaacccgcgc gaacgccctt accagcgctt cgccgccgcg acccggccgc
     2101 acctggccca ggtgcacgaa gagcggcgtg tcggcttcca gcccggtggc gctccgcagg
     2161 tacgggtcgg gccggacggg acccgcgacc ggggcgttca ggagcaccgt cggccgcgtg
     2221 atgccgtacg tccgggccag gtgatcggcg atggcgtccg agacggtcca gactaccgcg
     2281 gcctgccggg cgggggtggc ctcgaaggcg cgccagtacc ggctgacgag cggccggccc
     2341 gccgtggcgg cgacatgggc gtagagctcg cgggcgtcgt aagtgaacgg cgcccgggtg
     2401 cgggcggccg ccgagcgcac ggcccggagc acgtacaggt cgctcgcgtg gaagacggcc
     2461 gcatccacgc cgcggagcgc gcgcgtgaag gcgcggtgga tcgagtggaa gcggcgcggc
     2521 cccctgcgcc cgggttcggg cacgtacacc ggatccacgc cgtgcgggag cgtcgtgcgg
     2581 agcgtagtgg gggggaaggc gagaagcgtc accgtgaggc cggccccgca cagggcgtcg
     2641 agctgacgca gggcgcgcgt gttgcggtgg acgtcccccg tcatggcgaa cgccacgtcg
     2701 ctcggatgtc ttgcggtcac ggtggattcg aggttcatct acccttcgat tatagcactt
     2761 gaccgagaat cttcggacgc gtacactccc ggcagtgatg taagcgctta cacactatcc
     2821 ctcgtcggtc ccaccattcc ggcacaggat gggcgtcacc atctacgaca tcgcccgcga
     2881 ggcaggggtt tcgatcgcca cggtttcccg cgtcgtgaac ggacacgagc gagtggccga
     2941 agccacccgg gagcaggtgc tcgcggtggc gctcgagctc ggctaccagc cgcacgcgtc
     3001 cgcacgcagc ctggcgcgac gccgctcgaa cgtgatcgcg gccgtgatcc cgatgctcac
     3061 gaactacttc ttcatcgagg tgcttcgcgg cgtgcaggac cggatggcgg agagcgattt
     3121 cgacctgctc gtgttctcgg cccggacgct cgacgaggtg ggcgaccagc tggttcgagc
     3181 cctggccaag ggccgaagcg ccggggtgct gctcttctcg agcccgatgg acccggaacg
     3241 cacccgccag atcatcgctt cgggccggcc ggtcaccctc gtggacgaaa cccacgaggc
     3301 tttcgacgcg gtctcgaccg acaacctgct cggcggacgg ctcgcggtac gccacctgct
     3361 cgatcgcggc gcgcgccgca tcgcgctcat catggcgcgg ccggagtcca tcccggcggc
     3421 cgatcgccac gagggttacc ggatcgcgct cggcgagtac ggcatcgagt gcgatccggc
     3481 actcgtggtc gtctccgagg acggtctcta tcacggctat tccgaggccg cgggatacga
     3541 agcgatgcag cggctgctcc gcgcgcgcgg acccgcgccc gacgcggtct tcgcgacgag
     3601 cgacgtgcag gcgctcggcg ccatgcgggc gctgcaggag gcgggcatcc gggtcccgga
     3661 ggacatgctc gtcgtcgggt tcgacgacat catgatcagc cgccacctcg gactctcgac
     3721 cgtgcgccag cccatgtacg agatgggacg gctgggcgcc gacaagctgt tcgcccggat
     3781 cgagaatccc gaccacgagg tgtcccacac cattctcgcg ccgcggctcg tggttcggcg
     3841 ctcatcgggc gtgccgtcca ccgctccgac ccgggtcgcc tcgtgagccg tccggcatcc
     3901 atagcgtccc tgctggttct cggcgcggtc ctcgcggcct gcgggggcag cggagagcgg
     3961 attctcgccg aggtcggcga ccgggtcgtg acgcaggacg ggttccgcga cgcctacgtc
     4021 gattacctgg tgtccaccgg cctcccggac gatccggcgc gccgcacgag cttcctggag
     4081 aggctcgtct cgatggagct ggtgcggctc gacgcgctgg agcacggcct gggcgagggc
     4141 gacgcctggc gcgacgcgga ggaacgcgcg aggcggcggc tcctgctcga cgcctggatc
     4201 gccgaggtcg ccttcgacac gctgaccgtg gaccaggccg aactggagga cctgttcgtc
     4261 cgcgtcaaca ccgagatcac cgcccggcac ctgtacgcac cgacgcgcga ggaggccgag
     4321 cggctggccg accggctggc ggccggcgag acgttcgagg acctggcccg cgaggtgttc
     4381 gccgacccgg ccctggccgc cagcgggggg tcgctcggga ccttcggctt cgacgagatg
     4441 gacccggcct tcgaggaagt cgccttcacg ctggagccgg gaagcgtttc cgggcctgtc
     4501 aggacctcgc agggctggtc gatcctccgg gtcgacgacc ggttcacccg gcctcttctc
     4561 accgagaccg agttcgccgc ccggatgccg cagcttcgtg cgtacgtcct cgaacgcgaa
     4621 cggaagctgg cccggcggga ggtactcgag gagatgctga tctccacggc cccggagatc
     4681 gagccttccg tggccgaccg ggtgctgggc ctgctcggac ccgccgtcgt gggcgccgag
     4741 gacgtgaacg cggacgcggt gctcatccgg tacacggacg acggcgagtc cagggcgtgg
     4801 acggtcggcc agttcctcgc ggccgcccgg tacacggacc tggagcagcg ccgcgccgtc
     4861 cgcgaccggt ccaccttcga ggccttcgcg acgggtctcc tggtccgcga cgtgatcatg
     4921 gagcgctccc ggcaggccgg gctggaccgg tctgcccggt tccgggaggc gctcgattcg
     4981 gccatggacg aatggctcta cgagcgcgga tacgagcgga tcgcggaatc cgaacctgtt
     5041 ccagaggaca cccttcgtgc gttcttcgac cggcacgccg cggagctcgt cctgcccgag
     5101 cgcgtggccg tgcgcgagat cgtgactacg ggccgggcgg aggccgattc catccgcgcg
     5161 gccctctccg gcgcgtcgtt cgcggagctg gcccgccgcc actccgtgcg ctccgcgacg
     5221 tcaggggcgg gcggcgacct ggggctgctg tcgagggcgg atctcgggcc gcttgccgac
     5281 cgggtcttcg ccgcaccccg aggagcggtg atcggcccca tccccgttcc cgggcagtac
     5341 ctgctgctcg aggtcgggga gacctccgag tcccgccccg ccaccttcga cgaggcccgc
     5401 gagcagatcg aggccatcgt ccgccgcgaa cagcgcgacc gcctggtccg cgagcgagcg
     5461 gcgctcctgc gcgaccggtt cgcggtccgt tacgcggacg tggacctggc cgcgctcccg
     5521 ctccggtcgg agcccgttgt ttcatcctga tcgccatcac cccatgcgca agatttggtc
     5581 cgcaaccctc ctgcttgccg tgctcacggc cggaagcgcc gcggctcaga cgggcaagat
     5641 atcgggtcgc gtgctggacg cggccaccgg cgaatccctg ccgggcgtca acgttgtcat
     5701 agaggggacc atgcagggca ccgccacaaa cctggacggc gagtacgtga tcatcggcgt
     5761 gcggccgggg acgtacgacg tgctggcgtc cttcatcggg tactcgaccg agcgcaagga
     5821 gggcgtgcgc gtgaacctgg atctcacgac cacggtcgat ttcgacctgc aggaggaggt
     5881 catcatgggc gaggagatcg tggtcacggc cgaggcggtg ggcgtccgca aggacgtgac
     5941 ctcgtccgag gcccgcgtca cgtccgagac cctggaccgg ctgccggtga cggagctcgc
     6001 gcaggtgctg gaggtccagg ccggcatcac gaaccgggga gggcttcaca tccgcggcgg
     6061 acgctcgtcc gaggtgatgt tcatggtgga cggcgtgccg gtgaccgaca gctacgacgg
     6121 ttcgacctcg gtgcagctcg agaacgacgg catcgaggag ctgcaggtga tctccggcac
     6181 gttcaacg
//
LOCUS       DBNHQZ010000324         6203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2398619, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000324 DBNHQZ010000000
VERSION     DBNHQZ010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2398619"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="AC16YU_08675"
     CDS             complement(<1..927)
                     /locus_tag="AC16YU_08675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6065338.1"
                     /translation="MSTHATTETATGTLADPSTWMSGGAALPDGKAWTRHRPGAGPDH
                     MPLEDGSRVAVIGGGPAGSMFSYFLLRTLALTDTRIELDLYEPRDFNLTGPAGCNHCG
                     GIISESLVQILATEGIAIPSSVIQRGIDAYDLHMDIGTVRIRTPLNEKRIAAIHRSRG
                     PRNAVNGTHVGFDRHLLDLAVDRGAHVVRKLVSRIDWSGDRPEVECPDGHRAIYDLVV
                     VATGVNSAVVHMIEDLGLGYRAPGTVKTFISEFRLGREMIDRDFGNSMHVFLLDLPRL
                     EFAAAIPKGDYVTVAMLGNGVDEGLVESFLNTA"
     gene            complement(1060..2544)
                     /locus_tag="AC16YU_08680"
     CDS             complement(1060..2544)
                     /locus_tag="AC16YU_08680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6065339.1"
                     /translation="MTQSILVTGGTGYIGGRLVRRLLDRGHRVRCLVRDEARLASRSW
                     ADQVEVVRGDVLDPDSLRVAMDGIDAAYYLVHFHDVDTPDPAERNRRAAANFGQAAAA
                     AGLKLVIYLGSLPPVAGTPSRWVRQRIEAGVMLRRADVPVVEFRAGVIIGSGSLAFEM
                     IRYTVERVPLLVAPLWVRTKVQPIALRNVLQYLVRALDQPDCAGRIMQIGGPDILSYA
                     DMFRQYAEIRGLRRLMLTVPVPSVHLSALWLGLVTPIAGPVARRFLEGLTGDLVLTDD
                     LARRLFDVRLIGYRATVRAALRHFAEDQVDTSWTDALHAEPGGAEAVEKLASSEGVFV
                     ERIEVRTDVSARALFATICSIGGDSGWPYANALWKIRGMIDFLMGGVGMRSARRSVRA
                     LRVGDTLDFWRVEAIEPDRHLRLRAEMKVPGRAWLEYRLDAGEAGRTCVRQTALFEPR
                     GLAGILYWYLFYIPHRFIFPGMVRELIRRAAQNGVETREPGVEV"
     gene            complement(2623..5901)
                     /locus_tag="AC16YU_08685"
     CDS             complement(2623..5901)
                     /locus_tag="AC16YU_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6065340.1"
                     /translation="MSRIALLLALGILSIGESAAQTRLLRMPTISRDHVAFVYAGDVW
                     IADRSGGDARRLTTFDGAETWPALSPDGRTVAFSGQYDGNTDVYVVPVEGGEPTRLTW
                     HPAGDVVRGWTPDGRSILFESGRDNAPIPIGRFFTVPASGGFPEALPLDRAHRGSFSP
                     DGTRLAYQMIQPWEEEFRNYRGGQNGPIRILDLESLDVRTIPWDPDERPNDQQPAWLG
                     ETVYFLSDRDYAMNVWAFDGGTGEVRQLTHFADFDSKNLSAGGGALVFENGGWLYTLA
                     PGAGEPVRLDITVRGDFPWARPHWEDVAGDIRSGRVSPSGKRALFEARGDIFTVPAEK
                     GDVRNLTGTPGVADRAPAWSPDGQHIAWFSDESGEYALVVADQYGRDRRVIGLESPTF
                     FYAPVWSPDSKKIAFTDADRVLWVVDVAAGKKTRVDTEGRAHPERNIEPEWSSDSRWI
                     AYTRSLPNLYNAVFLYDVESGGQPVQLTDGLSNSLQPVFDAGGEYLYFLASTDYGLST
                     GWLDMTSYPFRASYAVYMAVLSAEAEHPFAPESDDEPEEGGEDEGEDDEGDDDDEEEE
                     EEEEEDEPEAVRIDLEGIGSRVIALDMPARRYVDLEPGKGGTLFAMANEEGKPELTLY
                     RFSVEDREAKEVMGGISGFDVSADGSKLIYVAPGDAWGLVDAAGEAKVGDGKLDTGSM
                     RARVDPTAEWRQIFREAVRFQRDYFYVDNVHGLDLAWAERTYGAWLEHVRHRDDLNYL
                     LDILGGETAIGHSFVGGGDTPDVESVPMGLLGADFAVEDGRYRIARIYSGEAWNPSLR
                     GPLSGPGIDAREGDWIVAVDGEPLAAGENIYGRFERTAGRQVKLTLNDRPTADGAREV
                     TVVPVPSESGLRQQAWVEGNRRRVDELSGGRLGYVWLPNTGDGGYANFNRYYFAQQDR
                     QGAVIDERFNGGGSAADYMVELMDRELMGYFSNPVGDRRPWTTPIAGIWGPKVMIIND
                     AAGSGGDLLPYMFRLRGIGPLVGTRTWGGLVGIWDVPRFIDGGGMTAPRGGFFDLEGE
                     WAVENEGVPPDIEVQMDPKAVAAGRDPQLEEAVRVALELLESDSFTLPAQPPDPVRVR
                     RPLR"
     gene            6051..>6203
                     /locus_tag="AC16YU_08690"
     CDS             6051..>6203
                     /locus_tag="AC16YU_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR6065341.1"
                     /translation="MSTDRRSFLRTAALAGAAVGLSPSAALTSPRRAPRRHRPSSSPL
                     RALVLGG"
BASE COUNT          934 a         2268 c         2022 g          979 t
ORIGIN      
        1 ggccgtgttc aggaaggact cgaccaggcc ctcgtccacc ccgttcccca gcatggcgac
       61 cgtcacgtaa tcgcccttcg ggatggccgc ggcgaactcc agccgcggca ggtccaggag
      121 gaatacgtgc atcgagttgc cgaagtcgcg atcgatcatc tcgcggccca gccggaactc
      181 ggagatgaac gtcttgacgg tccccggagc gcggtagccg aggccgagat cctcgatcat
      241 gtgcacgacg gcactgttca ccccggtcgc aacgaccacc aggtcgtaga tcgcccggtg
      301 accgtccggg cactcgacct ccggccggtc tccggaccag tcgatgcggc tcacgagctt
      361 gcgcacgacg tgagcgccgc ggtccaccgc gaggtccagc aggtggcggt caaatcccac
      421 atgcgtgccg ttcaccgcgt ttctcggccc ccggctgcgg tggatagccg cgatccgctt
      481 ctcgttgagc ggcgtgcgga tccgtaccgt accgatgtcc atgtgcagat cgtaggcatc
      541 gattcccctc tggatcacgc tggacgggat ggcgatgccc tcggtcgcca ggatctggac
      601 cagtgattcg gaaatgatgc cgccgcagtg gttgcatccc gccggccccg tcaggttgaa
      661 atcgcggggc tcgtacaggt ccagttcgat ccgggtatcg gtgagcgcca gggtgcgcag
      721 caggaaatag ctgaacatcg aacccgccgg gccgcccccg atgacggcga cccgggagcc
      781 gtcttccagc ggcatgtggt ccggtcccgc cccgggacga tgccgggtcc acgccttccc
      841 gtcgggaagc gcggcaccgc ccgacatcca tgtagacgga tccgccaggg tgccggtcgc
      901 cgtttcggtt gtcgcgtgcg tgctcatgat ccgcccgtgc gtgcgcggcg actcctgtcc
      961 cggacggctt gtcgcggtcg atgcgcctgc agcgcaaacg taattcgagc cgtcctctcc
     1021 gtcgtaaggc caggccggat cgccgggtag gaccatcccc tacacctcga ctcccggttc
     1081 gcgggtttcc acgccgttct gcgccgctct ccggatcagc tcgcgcacca tgccgggaaa
     1141 aatgaaccgg tgcgggatgt agaacaggta ccagtacagt atgccggcga ggccccgcgg
     1201 ctcgaacagc gccgtctgcc ggacgcatgt ccgaccggcc tcgcccgcgt ccagcctgta
     1261 ttcgagccac gctcggcccg gcaccttcat ttcggcccga agccgcagat gccggtcggg
     1321 ctcgatggcc tccacgcgcc agaaatccag cgtgtcgccg acgcggagcg cccgcaccga
     1381 gcgccgggca gagcgcatgc ccacgcctcc catcaggaag tcgatcatgc ccctgatctt
     1441 ccacagcgca ttcgcgtagg gccagcccga gtcgccgccg atggagcaga tggtggcgaa
     1501 cagggcccgc gccgacacgt cggtccgcac ttcgatgcgc tcgacgaaga ccccctcgct
     1561 cgatgccagc ttctccaccg cttccgcccc gccgggctcg gcgtggaggg cgtcggtcca
     1621 cgaggtgtcg acctggtcct ccgcgaagtg gcgcagggcg gcccgcacgg tagcccggta
     1681 gccgatgagc ctcacgtcga acagccggcg ggccaggtcg tccgtcagca ccaagtctcc
     1741 cgtcagccct tccaggaagc gccgggcgac cggccccgcg atcggcgtca ccagtcccag
     1801 ccacagggcg gagaggtgca cgctcggcac gggtacggtg agcatcagtc ggcgcaggcc
     1861 ccgtatctcc gcgtactgac ggaacatgtc ggcgtagctc aggatgtcgg gtcccccgat
     1921 ctgcatgatg cgacccgcgc agtccggctg gtccagggcc cggaccaggt actgcaggac
     1981 attgcgcagg gcgatgggct gcaccttggt ccgcacccac agcggcgcca cgagcagggg
     2041 cacgcgctcg accgtgtagc ggatcatctc gaacgcgagg ctccccgacc cgatgatcac
     2101 acctgcgcgg aactccacca cggggacgtc ggcgcgccgc agcatgaccc cggcctcgat
     2161 gcgctgacgc acccagcgcg acggcgtccc ggcgacggga gggaggctcc cgaggtagat
     2221 caccagcttc agacccgcgg ctgccgcggc ctgtccgaag tttgcggcgg ccctgcggtt
     2281 gcgctctgcc gggtcagggg tgtccacatc gtggaaatgc accaggtagt acgccgcatc
     2341 gatgccgtcc atcgcgactc ggagcgagtc cggatccagg acgtcccccc gcacgacctc
     2401 gacctggtcg gcccacgagc ggctcgcgag tcgcgcctcg tcgcggacga ggcaccgcac
     2461 ccggtgcccc cggtccagga gccgccgtac gagccttcct ccgatatacc cggttccccc
     2521 ggtgactaga atcgactgtg tcatcgtccg cgcggaaagc cggatgatag cgatccggat
     2581 ggggggtcca ccgaggggga atcccttaca ggaccgttat cctcatctca ggggcctccg
     2641 cacccgcacc ggatccggcg gctgcgccgg gagggtgaac gagtcgctct ccaggagctc
     2701 cagggctacg cgcacggctt cctccaactg ggggtctcgt cccgcggcca cggccttagg
     2761 gtccatctgc acctcgatgt cggggggcac gccctcgttc tcgacagccc actcgccctc
     2821 caggtcgaag aatccgccgc ggggcgcggt catgccgccc ccgtcgatga atctcgggac
     2881 gtcccagatg cctaccaggc cgccccaggt gcgggtgccc accaggggcc cgatgccgcg
     2941 cagccggaac atgtagggca acaggtcccc gcctgatccc gccgcgtcgt tgatgatcat
     3001 gaccttgggg ccccagatcc cggcgatcgg ggtcgtccag ggacgtcggt ccccgaccgg
     3061 gttgctgaag tagcccatga gctcgcggtc catgagctcg accatgtagt ccgcggccga
     3121 gccccctccg ttgaagcgct cgtcgatgac cgcgccctgg cggtcctgct gggcgaagta
     3181 gtagcggttg aagttcgcgt agccgccgtc gcccgtattc gggagccaga cgtaccccag
     3241 gcgaccgccc gagagctcgt cgacccgccg gcgattgccc tcgacccacg cctgctggcg
     3301 caggcccgac tcgctcggga ccggcaccac ggtgacctcg cgcgcgccgt ccgccgtcgg
     3361 acggtcgttc agggtcagct tcacctgccg gcccgccgtc cgctcgaacc ggccgtagat
     3421 gttctccccg gcggcgagcg gctccccgtc gacggcgacg atccagtctc cttccctcgc
     3481 gtcgatcccc gggcccgaga gcggtccgcg caggctcgga ttccaggcct ctcccgaata
     3541 gatccgggca atccggtaac ggccgtcctc gacggcgaag tcggccccca ggagacccat
     3601 cgggacgctc tccacgtcgg gagtgtcgcc gccgcccacg aaagagtgcc cgatcgcggt
     3661 ctcgccgccc agaatgtcca ggagatagtt caggtcgtcc cggtggcgaa cgtgctccag
     3721 ccaggccccg tacgtccgct cggcccaggc gagatcgagg ccgtggacat tatccacgta
     3781 gaagtagtcg cgctggaagc ggaccgcctc gcggaaaatc tggcgccatt cggccgtcgg
     3841 atccacccgg gcgcgcatgc tgcccgtgtc cagcttgccg tcgccgacct tcgcttcgcc
     3901 cgcggcgtcg accaggcccc acgcgtcgcc cggggccacg tagatcagct tgctgccgtc
     3961 ggcggacacg tcgaacccgg agatgcctcc catgacctcc ttcgcctcgc gatcctcgac
     4021 cgagaagcgg tacagggtga gctccggctt gccctcctcg ttcgccatgg cgaacagggt
     4081 gccccccttc ccgggctcca ggtccacgta gcgacgggcc ggcatgtcca gggcgatcac
     4141 ccggcttccg atgccttcga gatcgatgcg gacggcttcg ggctcgtcct cctcttcctc
     4201 ctcttcttcc tcttcgtcgt catcgtcccc ttcgtcgtcc tctccctcgt cctcaccgcc
     4261 ctcctccggc tcgtcatcac tctcgggcgc gaacggatgc tccgcctcgg ccgacagcac
     4321 ggccatgtag accgcgtagc tcgccctgaa cgggtaggac gtcatgtcca gccacccggt
     4381 cgagaggccg tagtcggtgg acgcgaggaa gtacaggtac tctccacccg cgtcgaacac
     4441 cggctgcagg ctgttcgaca ggccgtcggt gagctgcacc ggctggcctc ccgactcgac
     4501 atcgtacagg aagaccgcgt tgtacaggtt cgggagactg cgcgtgtacg cgatccagcg
     4561 cgagtcggac gaccactccg gctcgatgtt gcgctccggg tgcgccctgc cctcggtatc
     4621 gaccctcgtc ttcttgccgg ccgccacgtc cacgacccac agcacgcggt cggcgtcggt
     4681 gaaggcgatc ttcttcgagt cgggcgacca gacgggcgca tagaagaagg tgggcgattc
     4741 gagcccgatc acccgccggt cgcggccgta ctggtccgcg accacgagcg cgtattcacc
     4801 ggactcatcc gagaaccagg caatgtgctg cccgtcgggc gaccatgccg gcgcccggtc
     4861 cgccacgccc ggcgttcctg tcaggttgcg gacgtctccc ttctcggccg gcaccgtgaa
     4921 aatgtcgccc cgggcctcga agagcgcccg tttccccgac ggcgagacgc ggcccgaccg
     4981 gatgtcgccg gccacgtcct cccagtgtgg cctggcccac gggaagtcgc cccgtacggt
     5041 gatgtcgagc cgcaccggct ctccggcccc cggggccagc gtgtacagcc acccgccgtt
     5101 ctcgaagacg agcgcgccgc cgccggccga caggttcttg ctgtcgaaat cggcgaaatg
     5161 cgtgagctgc cggacctcgc ccgtccctcc gtcgaaggcc cagacgttca tggcgtagtc
     5221 acggtccgaa aggaaataga ccgtctcgcc gagccacgcg ggctgctggt cgttggggcg
     5281 ctcgtccgga tcccacggga tggtccgcac gtccagagac tccaggtcca ggatccggat
     5341 cgggccattc tggccgcccc ggtagttacg gaattcctct tcccagggct ggatcatctg
     5401 gtacgccagg cgggtcccgt ccgggctgaa ggagccgcgg tgcgcgcggt ccagcggcag
     5461 ggcctccggg aagccgcccg aggccggcac ggtgaagaag cggccgatcg gaatcggggc
     5521 attgtcgcgg ccggactcga agaggatgct ccgtccgtcc ggcgtccagc cacgcacgac
     5581 gtcgccggcc gggtgccagg tgagccgcgt gggctcgccc ccctcgaccg gcaccacgta
     5641 cacgtccgtg ttgccgtcgt actgtcccga gaaggccacg gtgcgaccgt ccggtgacag
     5701 ggcgggccac gtctccgcgc cgtcgaaggt cgtcagccgc cgggcgtccc cgccggagcg
     5761 gtccgctatc cagacgtctc ctgcgtacac gaaggcgacg tggtcgcggc tgatcgtggg
     5821 catgcggaga agtcgggtct gggcggcgga ctcgccgatc gacaggatac ccagggcgag
     5881 aagcagcgct atgcgggaca tggcattcac cgtgcggcgg tcagttttgc gggtgcagga
     5941 tacgcaccgg agaggagtcg ggcctccggg gattccccgg tgccgagtgc cgtgcggacg
     6001 gaatcggcgt gccgtaccat gtccggccga tccagcgacc cgcacggacc atgtccaccg
     6061 accgacgctc cttcctccgc accgcggcgc tcgccggcgc cgcggtgggc ctgtcaccct
     6121 ccgctgccct cacctcgccc cgtcgcgcgc cgcgccggca cagaccgtca tcttctcccc
     6181 tgcgcgcgct cgtgctcggc ggc
//
LOCUS       DBNHQZ010000325         5192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_366503, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000325 DBNHQZ010000000
VERSION     DBNHQZ010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_366503"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..4950
                     /locus_tag="AC16YU_08695"
     CDS             91..4950
                     /locus_tag="AC16YU_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR6065342.1"
                     /translation="MEGTWTDDDGDGAQDATDGDGIVQAGELVSYAFTVRNTGNVTLT
                     NVAVASSDVAVSGAALTSLAPGSEDATTFTGSHALTQADVDSGSFDASASATSNESDP
                     APGGAATPITQVPQLGLVNTATYDSGVITYTYVATNTGNLTLFDVVVDEALFTGTGPL
                     PDPVYVSGGADLDGEGDALDLSPGASITFVVAYTVTQDDIDAGGVTNQAEATSSYTDA
                     LGTPQTVTDLSDDTSPAGDSPTATPLVQNPALELVKSGVFQDEDGDGIASVGETIAYT
                     FTVTNAGNVTLTNVTVTDPMVAVSGGPIALAPGASDLATFSASYPLTAADLAAGAVVN
                     TATADSDESGPDESSVTTDLPIGIPFVFNEDGLPTATDGSDFDDLEAAFAAWESVPGT
                     TVRFRLGGTTSAQHADATDGVNLVTFRDDTFPFPPFVLAIASKTIVAGEVVDADIVFN
                     PAYVDNPLLKFRTSTGPADGNVDVQSTMTHEVGHVVNMLHSGVPDATMFFQLQPGNEA
                     RTLEPDDAAWITRRYPATGALEGLGFIDGRVLDGDQASDARGGVAGALLLARETTSGD
                     LFHTYSDADGFYAFLALPAGSYLVSVQPLDGDVGPLDETARYPMSAANVSAELRAITV
                     NTTFIPEYWNDGAESDDPETDLPGESVPVAVGAGSGASGIDIVTNADRTPPAVVGFIP
                     ADGSTAPATTDIIVSFSEPVDGSTVLFTFEDVGAGLFLTGTLTLHNDNTLGIFVPDSP
                     LVAGATYRMTVAGGMADQRGTVQSESFTSTFQTGAGTGSGFVVTSVGPADVPGATYAI
                     STPVYADFSDPVNRATVDLATFSVSSGGIPVPGIFDFVLGDTRAVFRAPLGLADDTEY
                     TLNIDGVENLGGESVTPWTSAFLTEGDFGPVIQTAAPSAAVVGTLVTLGGKGFDPDPA
                     MNTVTIAGVPAAVVHATLTGLTFEVPASVPEGDVDIVVENLTTAASGSGVPSTSSPFP
                     YTIVTGILLDDAAVARIVSESGSRDTDVSPDGALAYVTNTDNDWVTVIDLISLQVIGL
                     PIAVGDAPLKIVVHPGGSTAYVTNFLSHTVSVIDLTATPPVVVDEIPVGLNPIGIAAA
                     PDGSEIYVAEFTSQGVSVISADPTGGAYNQVVGRITTESSNRDVDVSPDGALAFVTGD
                     EGLAIVNLDPSQPDYNAVVARVSSETSTRDVDVSPDGALAFVTTEAGGIFVVDINPEN
                     ATYSVVARTSSETGAREVEVSPDGAMLFVTNYDGNSLDIYTISVGGSGSSGSGVTAPI
                     TTVPPVVSLTPAVPPSFVLGDGTGENPEGLVFSPKSSILVVANSTSGTVSIFRFGDDP
                     DRDDDGLLDRREALVGVFYEIIAGYGVNVDEELNKWRNGVYPGKSKDPRERILNQLLR
                     ALDSLVKSLDSSLWIDPFHLDPAQGGKLFDNDRQVVQSVVAVLAETIDDETRAQLQFI
                     IDEIIQIEIQLVTVLLDETVCSGDATCQRGYDTAVSELARAQDQYATGQYENAVRTLK
                     SAWGSAVSVAPSLARAALQLSLQASGLALSAGDSGALPTEFALRQNFPNPFNPVTTIR
                     YDVPEQAAVRVEVFDLLGRRVRLLVDGSLPAGYHEVRFDASTLSSGIYLARMTAGRFT
                     AVRRMVLVK"
BASE COUNT          811 a         1795 c         1792 g          794 t
ORIGIN      
        1 aacacggcca ccgtcgacac ggaccggacc gatccgcagc aggcctcggc gacgacgacc
       61 atcgcgcagg cgccctcgct cgagctcgtc atggagggca cgtggacgga cgacgacggc
      121 gacggcgcgc aggatgccac ggacggcgac ggcatcgtgc aggcgggtga gctcgtctcg
      181 tacgccttca cggtccggaa cacgggcaac gtcacgctta cgaacgtggc cgtcgcgagc
      241 agcgacgtcg cggtctcggg cgccgcgctc acgagcctcg cgccgggctc ggaggacgcg
      301 acgacgttca cgggcagcca tgcgctgacg caggccgacg tcgactcggg ctcgttcgac
      361 gcgtccgcga gcgcgacctc gaacgagtcg gatccggcgc cgggcggagc ggccacgccc
      421 atcacccagg tgccgcagct cggcctcgtg aacaccgcca cgtacgattc gggcgtgatc
      481 acgtacacct acgtcgccac caacacgggc aacctcacgc tcttcgacgt ggtggtcgac
      541 gaggcgctct tcactggcac gggaccgctt cccgatccgg tctacgtgtc cggcggcgcc
      601 gacctggacg gagagggcga tgcgctcgac ctgtcgccgg gcgcgagcat cacgttcgtg
      661 gtcgcctaca cggtgacgca ggacgacatc gacgcgggtg gagtgacgaa ccaggccgag
      721 gccacgagca gctacacgga tgcgctcggc acaccccaga cggtcaccga cctttctgac
      781 gacacctctc cggccggcga cagcccgacc gccacgccgc tcgtgcagaa tccggcgctc
      841 gagctggtca agtcgggtgt cttccaggac gaagacggcg acggaatcgc gagcgtcggc
      901 gagaccatcg cgtacacctt cacggtcacg aatgcgggca acgtgaccct gaccaacgtg
      961 acggtgaccg atccgatggt ggcagtgtcg ggcggcccta tcgcgctcgc accgggcgca
     1021 tccgacctgg cgacgttttc cgcgagctat ccgctcacgg ccgccgacct cgccgccggg
     1081 gccgtggtca acaccgcgac ggcggactcg gacgagtcgg gacccgacga atcctcggtc
     1141 acgaccgacc tgccgatcgg gattcccttc gtgttcaacg aggacggcct gcccacggcc
     1201 acggacggct cggatttcga cgatctcgag gcggccttcg cggcctggga aagcgtccct
     1261 ggaacgacgg tgcgattccg tctcgggggg accacgtcgg cgcagcatgc cgacgccacg
     1321 gacggcgtga acctcgtgac cttcagggac gacacgttcc cgttcccgcc cttcgtgctc
     1381 gcgatcgcct ccaagacgat cgtggcgggc gaggtcgtgg atgcggacat cgtcttcaat
     1441 ccggcatacg tcgacaaccc cctgctcaag ttccgcacgt cgacgggtcc cgccgacgga
     1501 aacgtggacg ttcagagcac gatgacccac gaggtaggtc acgtggtcaa catgctgcac
     1561 agcggcgtgc ccgacgccac gatgttcttc cagctgcagc ccggcaacga ggccaggacg
     1621 ctcgagccgg atgacgcggc gtggatcacg cggcgctacc cggcgaccgg tgcgctcgag
     1681 gggctcggct tcatcgacgg ccgggttctg gacggcgacc aggcctccga cgcgcgcggc
     1741 ggcgtggcgg gcgccctgct gctcgcacgc gagacgacct cgggcgacct cttccacacg
     1801 tactcggacg cggacggctt ctacgccttc ctggcgctgc cggcgggctc gtacctcgtg
     1861 tcggtccagc cgctcgacgg tgacgtcggg ccgctcgacg agacggccag gtaccccatg
     1921 tcggcggcca acgtaagcgc cgagctccgg gcgatcacgg tgaacaccac cttcatcccc
     1981 gaatactgga acgacggggc cgaatccgac gacccggaga cagatctgcc gggcgagtcc
     2041 gttcccgtcg cggtcggagc gggcagcggc gcctccggca tcgacatcgt cacgaatgcc
     2101 gaccggacgc ccccggcggt cgtcgggttc atcccggcgg acggctcgac cgcgccggcg
     2161 accaccgaca tcatcgtctc gttcagcgag ccggtcgacg ggagcacggt cctcttcacc
     2221 ttcgaggacg tgggggcagg gttgtttctc acgggaacgc tcacgctcca caacgacaat
     2281 accctgggca tcttcgtgcc cgactcgccc ctcgtcgccg gcgccacgta ccggatgacc
     2341 gtcgccggcg ggatggccga ccagcggggc acggttcagt ccgaatcctt cacgtcgacc
     2401 ttccagaccg gggcgggcac gggctcggga ttcgtggtca cctcggtcgg ccccgcggac
     2461 gttcccggag ccacctacgc catctccacg ccggtctatg cagacttctc ggatccggtg
     2521 aaccgggcca cggtggacct cgccacgttc tcggtttcgt ccggcgggat ccccgtaccg
     2581 ggcatcttcg acttcgtgct gggcgacacg cgcgccgtgt tcagggcgcc tctggggctg
     2641 gcggatgaca cggagtacac gttgaacatc gacggcgtcg agaacctcgg cggagagtcg
     2701 gtcaccccgt ggaccagcgc gttcctgacg gagggcgact tcggccccgt catccagact
     2761 gccgccccgt cggcggcggt ggtcgggacg ctggtgacgc tcggcgggaa gggattcgat
     2821 cccgatccgg ccatgaacac ggtgacgatc gccggcgtgc cggcggcggt cgtgcacgcg
     2881 acgctcacgg gcctgacgtt cgaggttccg gcctccgtcc ccgagggaga cgtggacatc
     2941 gtcgtggaga acctcacgac cgcggcctcc ggttccggtg tgccgagcac gagcagcccg
     3001 ttcccctaca cgatcgtcac cgggatcctg ctcgatgacg cggccgtggc gcggatcgtg
     3061 tcggagtccg ggtcgcggga cacggacgtg tcgccggacg gagccctggc ctacgtcacg
     3121 aataccgaca acgactgggt cacggtcatc gacctgatca gcctgcaggt gatcgggttg
     3181 ccgatcgcgg tgggggatgc gccgctcaag atcgtcgtgc acccgggcgg gtccacggcc
     3241 tacgtgacga acttcctctc gcacaccgtt tcggtgatcg acctcacggc caccccgccg
     3301 gtcgtcgtcg acgagatccc ggtgggcctg aatccgatcg gcatagcggc ggcgccggac
     3361 gggtccgaga tctacgtggc cgagttcacg tcgcagggcg tatcggtcat ctccgccgat
     3421 ccgacgggcg gggcgtacaa ccaggtcgtg ggccggatca cgacggagtc gtcgaaccgc
     3481 gacgtcgacg tgagccccga cggggcgctc gcgttcgtca cgggcgacga gggcctcgcc
     3541 atcgtgaacc tggatccctc gcagccggac tacaacgccg tcgtggcccg cgtgtccagc
     3601 gagacgtcga cccgggacgt ggacgtatcg ccggacgggg cgctcgcgtt cgtgacgacc
     3661 gaggcgggcg ggatcttcgt cgtggacata aacccggaaa acgcgacgta ctcggtcgtg
     3721 gcccggacct cctccgagac gggcgcccgc gaggtggagg tgtcgccgga cggcgcgatg
     3781 ctgttcgtga ccaactacga cgggaattcc ctcgacatct acacgatcag cgtcggcggc
     3841 tcgggctcgt cggggtccgg cgtcacggcg ccgatcacga cggtgccgcc ggtcgtttcg
     3901 ctgacgccgg ccgtgccgcc ctccttcgtc ctgggtgacg ggaccggcga gaaccccgag
     3961 ggcctggtct tcagtccgaa gtcctcgatc ctggtcgtgg cgaactcgac gtccggcacg
     4021 gtctcgatct tccgcttcgg cgacgatccc gaccgggacg acgacggcct gctggatcgg
     4081 agggaggcgc tggtcggcgt gttctacgag atcatcgcgg gctacggcgt caacgtcgac
     4141 gaggagttga acaagtggcg caacggcgtc tacccgggca agtccaagga ccctcgtgag
     4201 cgcatcctga accagctcct gcgcgcgctg gattcgctgg tgaagtcgct cgactcgtcg
     4261 ctgtggatcg atccgttcca cctggatccg gcgcagggcg gcaagctctt cgacaacgac
     4321 cggcaggtgg tgcagagcgt ggtggctgtc ctcgccgaga cgatcgacga cgagacgcgt
     4381 gcgcagctgc agttcatcat cgacgagatc atccagatcg agatccagct cgtcaccgtg
     4441 ctgctcgacg agacggtctg ctccggcgac gcgacctgcc agcggggata cgatacggcg
     4501 gtctcggagc tcgcccgggc ccaggaccag tacgccaccg gccagtacga gaacgcggtg
     4561 aggacgctca agtccgcctg gggctcggcc gtctcggtcg cgccttcgtt ggcccgagcc
     4621 gcgttgcagc tctcgctcca ggcgtcgggt ctcgccctgt ccgcgggcga ttccggtgcg
     4681 ctgccgaccg agttcgcgct ccggcagaac ttcccgaacc cgttcaatcc cgtgaccacg
     4741 atccggtacg acgtgcctga gcaggccgcc gtgcgggtgg aggtgttcga cctcctgggc
     4801 cgccgggtcc ggctgctggt cgacggcagc ctgcccgcgg gatatcacga ggtgcggttc
     4861 gacgcttcga cgctctcgag cggcatctac ctcgcgagga tgacggccgg ccgattcacg
     4921 gccgtgaggc ggatggtgct ggtcaagtag gcgcccctgc cggggacgcc gcggcgcgtc
     4981 cgggtcctga atgagagaag cccccggctc ttcgcggagt cgggggcttc gcgtttccgg
     5041 gaatcagagg ccgatcgcgg cgaagatccg gtcggcctcg gcccgacgca ccgggtcgcg
     5101 ctccacctcg aggaagcggt cacggaacgg ccgggcgagg gcgggattgc ccagggcggc
     5161 atgcgcgcgg gccatgagga ggagcgtctc gg
//
LOCUS       DBNHQZ010000326         8237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_95862, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000326 DBNHQZ010000000
VERSION     DBNHQZ010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_95862"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1424
                     /locus_tag="AC16YU_08700"
     CDS             414..1424
                     /locus_tag="AC16YU_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6065343.1"
                     /translation="MGFLNFASDVAIDLGTANTLIYIKGRGIVLNEPSIVAIHRVTRK
                     VIAVGHEAQQMHERTHRDIETIRPLKDGVIADFEVAEQLIRHLIRKVQTSWVTAIRKM
                     VVCVPSGITEVEKRAVRDSAEHAGARQVYLIDEPMAAAIGIGLNVAEAVGNMIVDVGG
                     GTTEIAVIALSGIVIDESIRVGGDELDLAIVQYFKRNHNLLIGQRTAERIKMEVGSAV
                     ELDPELELSVKGRDLVSGIPKIRTISSEDVREALRDPVSQIAAAVIRCLERTPPELGS
                     DILERGIMLTGGGSLLKGLDILIRGRVDLPVFVAEDPLTAVVRGTGTVLEDIPGYDKV
                     LT"
     gene            complement(1403..2020)
                     /locus_tag="AC16YU_08705"
     CDS             complement(1403..2020)
                     /locus_tag="AC16YU_08705"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR6065344.1"
                     /translation="MPDPFTILDLPGATVALHERFLSESEAGALFDTLRTGVRWEQHT
                     VRLFGREVPSPRLSAWYGDAEAVYRYSGVTHRPHPWIPELEELRSRVERETGSSFNSV
                     LLNRYRDGSDAMGWHADDEPELGPEPTIASVSLGAARRMRFRHRTRKETTASVTLSHG
                     SLLVMAGATQRHWLHSVPRTRTVHSERINLTFRRILAARQVRTLS"
     gene            complement(2316..3278)
                     /locus_tag="AC16YU_08710"
     CDS             complement(2316..3278)
                     /locus_tag="AC16YU_08710"
                     /inference="COORDINATES: protein motif:HMM:NF020448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HIR6065345.1"
                     /translation="MAHRLIVTNGAAAAEAIRATGVAGEVCAWNDVLTDGPVPGSLDD
                     SALARVRAQFIADRGWGAVEEIHRWFSERDAQLEAAPNFPEVVLWFEHDLYDQLQLIQ
                     ILARLANGRSNVSLIQHRRFIGRSTAEELKADFKSRTPATQAQFGSATMAWAAFRSST
                     PAAVEALLASGRTEPLPFLSPALRRWCSTFPDPERGLSRTERRTLELVAAGTDSPAVL
                     FRKTSDEEEAAFRGDASYHVLLQELSSGPRPLLRTTSGLPLDPNAVSDARLAVTDDGM
                     GVIAGSLDRIEIGGIDAWRGGIHLTTASHWRWSSATGFELRVAG"
     gene            3548..6196
                     /locus_tag="AC16YU_08715"
     CDS             3548..6196
                     /locus_tag="AC16YU_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065346.1"
                     /translation="MPNRFRLTASLLALLLVPGSLTAQDLPPHVNQHALNRPLPSLHA
                     SAQRARGAGAPFTGMLIEIPNKAFKGDTRTQTGTGTQTGIVQTSVTSGSPVSPLRSFD
                     GADNNDNARLLGYRMAPPDTDGDVGPNHYVQMINVVTAVYDKFGNELGGGPFPSNAFW
                     AGMGGACETYNRGDPIVLYDEAADRWLVSQFAFSSVSGPPYYQCVAVSQTGDPLGAYN
                     RYGFDFSDIGFNDYPKLGFTSDAVTLMANIFAPPTLAFAGAFLGAIDKAAMYEGRDAA
                     MVGAVVSGWGFASADLDDPTGTAAHVPPLFATAMMRGGYLDIWQVDADFAAGTGSVFR
                     AAEVPIAPFGSDLCAASREACITQPAPGNSLEALSGRLMHRLAVRDFGTHLSMVATHT
                     VDAGGGRAGIRWYEIRNYGSGWELHQQSTFAPNDGLHRWMPSIAMNAAGDIGLGYLVA
                     STTQFMSIGVVGQTAAASGSGQMDADEVLCVQGTGSQTNSNRAGDYSATKVDPQTDTF
                     WHTNEYVRDTGSFTWVTRVCEFSVSGTVEPLPEPIPEPTPEPTPEPTPEPTPEPEHTE
                     QYVWSIVTGTSAGSKGRKYGTATVTILDDQGAPVGGAWVTGRFSGDFVESGGGRTAPD
                     GTLAFQTSSSSKIRGEGSIRFCVADVAHPELQYNPERNPVASYQCPDAATDGSADVHV
                     DAVVAAVVNGSKGTKLGSAGVTIRDSNGNVAAGYTVTGTFYGSLTEGPVEAVTDGAGV
                     AAFRTSGTLDASGTVGFCVGAVSGPLAYDPNDNLDPGFDCSSGKGDTYGFTETRPAGF
                     TLDQNYPNPFNPTTIIPFMVTESLPVRLAVYDLLGREVAVLVDEVVSGGLHRAAFDAS
                     RLPAGLYLYVLDAGGEREVRRMTLLK"
     gene            6383..6988
                     /locus_tag="AC16YU_08720"
     CDS             6383..6988
                     /locus_tag="AC16YU_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065347.1"
                     /translation="MSLRIPAAALALLLSGACSSSRPAGRSVFHFQVDSTTYEVISLS
                     LASGEGVNYLLRREGDEVVFRARDDDQDGRIDQVLIGDESLRTADSLYALGIEEAKRR
                     GQFAKREASRVFVVSREDGVYSIRSVMLGPSNWYNAFTVSDPVGSREVFLLDSDADGV
                     LDPTELDDASMRHWQSRYREMLEEGIRSMRVERTLSGYVVL"
     gene            complement(7088..>8237)
                     /locus_tag="AC16YU_08725"
     CDS             complement(7088..>8237)
                     /locus_tag="AC16YU_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR6065348.1"
                     /translation="KVIFDHVNNHIGIRHPWVDDPPRASWLNGTRGDHLRGQHYKLSL
                     WDAWGDPATVDRLTRFWFSDSMPDLDQRDPFVARYLVQNTIWWIETAGLDGIREDTYP
                     YPDQAFMRDWVRAVLDEYPGFNIVGEIWEPHSAYLAAWQAGSPVPGALDTGLPTVMDF
                     ALAEAMRSYMAGEAGLSGIYAVLAHDILYADPSRLLTFFDNHDMSRGYLVTRDDPRRA
                     HQMLALLLTTRGIPQLLYGTELGMVAGESHVELRADFPGGFPGDGRSAFTREGRTMGE
                     NKAFDLTRALLHLRSRYSALRTGELVHWPPTWGDDVYRFVRRDADATILVLVSGDDGD
                     REVSLDLLAHRLPAGAAFRDLLTGELVDPSTGDGIPVPALGFRILLVE"
BASE COUNT         1340 a         2825 c         2768 g         1304 t
ORIGIN      
        1 acgcccaacg aagacctgcg ggatttatcg ccggccggaa gaagtttccg ttcgtggccg
       61 cattattcct gctcaaaccc cctttccaac caccgaatcc gcctttcggg tgccattccc
      121 gtttggcccg cagtttgatt gaggctggct gccagaacac cgcgtcgtgt cggaccggcc
      181 ccgtaaggcc atttcggctc gacggaggct acccggcccc gtaaggccgg gtagtcgtgt
      241 ttcgggcccc ggccgatcgc ggtcgcggat cccgtcccgt ccttcgggaa agggcgagac
      301 tcccgatttg cgcgttggat gcgaggcctt ccctgtttat tttcggcgac cccgaaccgg
      361 ccctcgaacg cacctcctcc gggtcgcggg ttcctgattg acagcgggac gagatgggct
      421 tcctcaactt tgcgtcggac gtagcaatcg accttggcac ggccaatacc ctgatctaca
      481 tcaagggaag ggggatcgtg cttaacgagc cgagcatcgt ggccatccac cgggtcaccc
      541 gcaaggtgat cgcggtgggt cacgaggccc agcagatgca cgagcggacg caccgtgaca
      601 tcgagaccat ccggccgctc aaggacggtg tcatcgcgga tttcgaggtg gccgagcagc
      661 tgatccgcca cctgatccgc aaggtccaga ccagctgggt cacggcgatc cgcaagatgg
      721 tggtgtgcgt gcccagcggc atcacggagg tggagaagcg tgccgtgcgc gactcggccg
      781 agcacgcggg cgcccgccag gtgtatctca tcgacgagcc gatggcggcg gcgatcggga
      841 tcggcctgaa cgtggccgag gcggtgggca acatgatcgt ggacgtcggc ggcggcacga
      901 ccgagatcgc ggtcatcgcg ctctcgggca tcgtgatcga cgagtcgatc cgcgtggggg
      961 gcgacgagct ggacctcgcc atcgtccagt atttcaagcg caaccacaac ctcctgatcg
     1021 gacagcgcac ggccgagcgg atcaaaatgg aggtgggcag cgccgtcgag ctcgatccgg
     1081 agctcgagct ctcggtcaag ggccgggacc tggtctccgg catcccgaag atccggacca
     1141 tatcgtcgga ggacgtgcgc gaggcgctcc gcgatccggt cagccagatc gccgccgcgg
     1201 tcatccgctg cctggagcgc acgcccccgg aactgggctc ggacatcctc gagcgcggca
     1261 tcatgctcac cggtggaggg tcgctcctga aggggctcga catcctgatc cgcggccggg
     1321 tggacctgcc ggtcttcgtt gccgaggacc ccctgacggc cgtcgtccgt ggcaccggga
     1381 cggtgctcga ggacattccg ggctacgaca aggtcctcac ctgacgcgcg gccaggatcc
     1441 ggcggaaggt caggttgatc cgttccgagt ggaccgtccg cgtgcggggc accgagtgaa
     1501 gccagtggcg ctgcgtggcc ccggccatga cgagcagcga gccgtgcgag agcgtcaccg
     1561 acgcggtcgt ctccttccgg gtccggtgac ggaaccgcat cctccgcgcc gcacccagcg
     1621 aaaccgaagc gatcgtcggc tcgggaccca gttcgggctc gtcgtcggca tgccagccca
     1681 tggcgtccga cccgtcgcga taccggttga gcagaaccga gttgaagctc gacccggtct
     1741 cgcgctccac cctcgagcgg agctcctcca gttcgggaat ccacgggtgt ggccgatgcg
     1801 tgacgcccga gtaccggtag accgcctcgg catctccgta ccaggccgag agcctcggac
     1861 tcggcacctc gcgtccgaac agccggaccg tgtgctgctc ccaccggacg cccgtgcgaa
     1921 gcgtgtcgaa cagcgctccg gcctccgatt cggacaggaa tcgctcgtgg agcgccacgg
     1981 tcgcacccgg caggtcgagt atggtgaatg gatcgggcat tcggtggaac ggtgcggcga
     2041 gtactcccct cttcggcacc ggttgtccac actgcccggg cgggcagatc gacgcccttc
     2101 ggcggggcag atcgcaggac aagcgattgg cctggcccac aaatcgtgaa attacaagat
     2161 cttttgccgc gaatggattg tcaattccct gttacgccag cgatttcgtc cagctctccg
     2221 acttatccac acttcgtcca caaaccgctg gcgaattttc gcgcgtgccg tgaatcgtcc
     2281 cgcgcaccac cgtggaacct tccccggcgc agggttcagc cggccacgcg aagctcgaaa
     2341 cccgtggccg acgaccagcg ccagtgactg gcggtcgtga ggtgaatccc tccccgccag
     2401 gcgtcgattc cgccgatctc gatccggtcc aggcttccgg caatcacacc catcccatcg
     2461 tccgtgacgg ccagtcgggc atccgagacc gcgttcggat ccagcggcag acccgaggtg
     2521 gtccgcagaa gcggacgcgg cccggacgac agttcctgca ggagaacatg gtacgatgcg
     2581 tctccgcgga aggccgcctc ctcctcgtcg ctggtcttcc tgaacaggac tgccggcgag
     2641 tcggtccccg cggccacgag ctccagggtc ctgcgctcgg tgcgcgacag cccgcgctcc
     2701 gggtccggga aggtcgagca ccagcgtcgg agggccggtg agaggaaggg cagcggctcc
     2761 gttcgtccgg aggccaggag cgcctccacg gcggccggcg tcgaggagcg gaaggcggcc
     2821 cacgccatgg tcgccgaccc gaactgggcc tgggtcgcgg gggtgcggga cttgaaatcg
     2881 gccttgagct cctcggccgt cgagcgcccg atgaaccggc ggtgctggat cagcgagacg
     2941 ttcgaacgcc cgttcgcgag gcgcgccagg atctggatga gctgcagctg gtcgtacagg
     3001 tcgtgctcga accagagcac gacctccggg aagttcggag cggcctcgag ctgcgcgtcg
     3061 cgctcggaga accaccggtg gatctcctcg accgcacccc atccgcggtc ggcgatgaac
     3121 tgcgcgcgga cgcgggccag ggccgagtcg tccaggctgc cgggcaccgg tccgtcggtg
     3181 agcacgtcgt tccaggcgca cacctcgccc gcgacgccgg ttgcccggat ggcctcggcg
     3241 gcggcggcgc cgttcgtcac gatgagtcga tgagccacgg gcatccttcc tgttgaccga
     3301 cggcccgtcc aacgtcgggg cgcgcggccc cggttcccga tcgggcccac ttcggcacga
     3361 tcccggttaa aagcacctcc cgcctgccga taccggggac agatgggcga ttgaagcggc
     3421 cgactccctc agacgccgcg acaccgcctg gtatgctgac atctggcacg gcatacgcag
     3481 tcgggctgtc tggggaccag gcagaccggg cgctccgtgc ctccaccaag agtacggacc
     3541 tcccgagatg ccgaaccgct tccgactgac cgcatccctt ctcgccctgc ttctcgtccc
     3601 cggctcgctg acggcacagg atctcccccc gcacgtcaat cagcatgcac tgaaccgccc
     3661 gctcccgtcg ctgcatgcgt cggcccagcg ggcccgcgga gccggcgcgc cgttcacggg
     3721 catgctgatc gagatcccga acaaggcctt caaaggagat acccgtacgc agactggtac
     3781 ggggacccag acgggcatcg tccagacgag cgtaacgtcg ggctccccag tcagcccgct
     3841 ccggagcttc gacggtgcgg acaacaacga caacgcacgg ctgctcggct accggatggc
     3901 cccgcccgat acggacggag acgtgggccc gaatcactac gtgcagatga tcaacgtggt
     3961 gacggccgtc tacgacaagt tcggcaacga gctcgggggc ggtcccttcc cgagcaatgc
     4021 cttctgggcg ggcatgggcg gagcctgcga gacctacaat cgcggcgacc ccatcgtgct
     4081 ctacgacgag gcggcggacc gctggctcgt gagccagttc gcgttctcga gcgtatctgg
     4141 tcccccgtac taccagtgcg tcgcggtgtc ccagacgggc gatccgctcg gcgcctacaa
     4201 ccggtacggc ttcgacttct cggacatcgg attcaacgac tatccgaagc tcggattcac
     4261 gtccgacgcg gtcacgctca tggcgaacat cttcgccccg ccgacgctcg cgttcgccgg
     4321 ggcgttcctg ggcgcgatcg acaaggcggc gatgtacgaa gggcgtgacg cagccatggt
     4381 cggagccgtg gtcagcggct ggggcttcgc ctcggccgac ctggacgatc cgaccggcac
     4441 ggccgcccac gttccgccgc tcttcgcgac agccatgatg cggggcggat acctggacat
     4501 atggcaggtg gacgcggact tcgccgcggg cacgggcagc gtgttccgcg ccgccgaggt
     4561 cccgatcgct ccgttcggca gcgacctgtg cgcggcctcg cgggaggcct gcatcaccca
     4621 gcccgcgccg ggcaactcgc tcgaggccct ctccggacgt ctcatgcacc ggctggccgt
     4681 ccgcgacttc gggacccacc tgtccatggt cgcgactcat accgtcgacg cgggcggcgg
     4741 acgtgccggc atccgctggt acgaaatcag gaactacgga tccggatggg agctgcacca
     4801 gcagagcacc tttgcgccca acgacgggct gcatcggtgg atgccgtcca tcgccatgaa
     4861 cgccgccggg gacatcggcc tcggctacct ggtggcttcc accacccagt tcatgtcgat
     4921 cggagtggtc ggccagaccg cggcggcctc cggatccgga cagatggatg cggacgaggt
     4981 gctgtgcgtc cagggaacgg gctcgcagac gaactcgaac cgcgcgggcg actactcggc
     5041 cacaaaggtc gatccccaga ccgacacgtt ctggcacacg aacgagtacg tgagggacac
     5101 ggggagcttc acgtgggtga cccgggtctg cgagttcagc gtgtcgggaa cggtggagcc
     5161 tctccccgag ccgatacccg agcccacgcc ggaaccgacc cccgagccca cccccgaacc
     5221 cacgccggag ccggaacaca ccgagcagta cgtctggtcg atcgtgacag gtacctcggc
     5281 cggcagcaag ggccgcaagt acgggacggc gacggtcacg atcctggacg accagggcgc
     5341 accggtgggc ggagcgtggg tgaccggacg cttctcgggt gacttcgtcg agtcgggtgg
     5401 gggccggacg gctccggacg gcacgctggc cttccagacc tcctcttcga gcaagatcag
     5461 gggcgagggc tcgatccgct tctgcgtcgc ggacgtggcc catcccgagc tccagtacaa
     5521 tcccgagcgg aacccggtcg cgtcgtacca gtgccccgac gccgccaccg acggcagcgc
     5581 ggacgtccac gtggacgccg tggtcgccgc cgtcgtgaac ggttcgaagg gcaccaagct
     5641 gggcagcgcg ggcgtcacca tccgtgacag caacggcaac gtcgccgcgg gatacaccgt
     5701 gacgggcacg ttctacggct cccttaccga agggccggtc gaggccgtca ccgatggcgc
     5761 cggagtggcg gcgttccgga cgtccggcac cctggacgcc tccggcacgg tcggattctg
     5821 cgtcggcgcc gtgagcggcc ccctcgcgta cgatccgaac gacaatctgg acccgggctt
     5881 cgactgctcg tctggcaagg gagacacgta cggattcacg gagactcgcc cggcaggatt
     5941 cacgctggac cagaactacc cgaacccgtt caatccgacc accatcattc cgttcatggt
     6001 caccgagtcc cttcccgtgc gactggccgt gtacgacctg ctcggccgtg aagtagccgt
     6061 gctggtggac gaggtggttt ccggcggtct gcaccgggcg gcgttcgacg catctcggct
     6121 cccggccggg ctgtacctgt acgtcctgga tgcgggcgga gagcgcgagg tacgccggat
     6181 gacgctcctg aaataggatt ccggtcggcc gacggaccgc ggtccggttc ctcctcgggg
     6241 ccggaccgcg gtcctttcct acagcttcgt ccggctttgc cctatgggcc ggatggggat
     6301 gaccacggca accggcggtg cggcctcctc gtaggtatag ggtgaggtcg tgtatcgcgg
     6361 cctcttcatc cgcgaggacg ccatgtcgct ccgcataccg gccgccgcgc tcgccctgct
     6421 cctgtccggg gcctgcagtt cgtcgcgtcc agccggccgc agcgtgttcc acttccaggt
     6481 cgactcgacc acctacgagg tgatcagcct gagcctcgcc tcgggcgagg gggtcaatta
     6541 cctgctccgg agggaaggcg acgaggtcgt cttccgcgcg agggacgacg accaggacgg
     6601 tcgcatcgac caggtcctga tcggggacga gtcgcttcgc acggccgatt cgctgtacgc
     6661 cctcggcatc gaagaggcca agcggcgcgg gcagttcgcc aagcgcgagg catcgcgcgt
     6721 gttcgtcgtc tcgcgagagg acggagtgta ctcgatccgc agcgtgatgc tcggcccgtc
     6781 gaactggtac aacgccttca ccgtcagcga ccccgtgggg tcacgcgagg tattcctgct
     6841 ggactcggac gccgacgggg tcctggatcc gaccgagctc gacgacgcgt cgatgcgtca
     6901 ctggcagtcc cggtaccgcg agatgctcga ggaaggcatc cggtcgatgc gagtcgagcg
     6961 gacgctctcc gggtacgtgg tcctgtagcc cgccccgtcg ccgcggctga tcgacgaccg
     7021 gtcgttcacg cccaccgcac aggtccgctt cttccggcgg aaaatgcagg agatgggccg
     7081 ctgagtctca ctcgacgagc aggatccgga agccgagggc cgggaccggg atgccgtcgc
     7141 ccgtggacgg atcgacgagc tccccggtga gcaggtcgcg gaacgctgcg cccgcgggca
     7201 gccggtgcgc gaggagatcc agcgacacct cccggtcccc gtcatcgccg ctgacgagta
     7261 ccaggatcgt cgcgtcggcg tcccttcgta cgaaccggta cacgtcgtcg ccccaggtcg
     7321 gcggccagtg cacgagctct cccgtgcgga gcgcggagta ccggctccgg aggtgcagca
     7381 gtgcccgcgt gaggtcgaat gccttgttct cccccatcgt gcgcccttcc cgggtaaacg
     7441 cgcttcgccc gtcacccggg aagcctccgg ggaagtccgc ccgcagctcc acgtgcgatt
     7501 cgcccgccac catgccgagc tccgtgccgt agagcagctg cgggatgccc ctcgtggtca
     7561 ggaggagcgc cagcatctga tgcgcccggc gcgggtcgtc cctcgtgacc aggtacccgc
     7621 ggctcatgtc gtggttgtcg aaaaaggtca ggaggcgcga cggatccgcg tacaggatgt
     7681 cgtgggcgag caccgcgtag atgccggaca gacccgcctc gccggccatg tacgagcgca
     7741 tcgcctcggc gagcgcgaag tccatcaccg tgggcagtcc cgtgtccagt gcgccaggca
     7801 ccgggctgcc cgcctgccag gccgccaggt aggccgaatg gggctcccag atctcaccca
     7861 cgatgttgaa accggggtat tcgtcgagca cggcccggac ccagtcgcgc atgaaggcct
     7921 ggtcggggta cggatacgta tcctcgcgga tgccgtccag ccccgcggtc tcgatccacc
     7981 atatggtgtt ctggaccagg taacgcgcga cgaacgggtc gcgctggtcc aggtccggca
     8041 tcgaatccga gaaccagaac cgggtgagcc ggtcgacggt cgcggggtcg ccccaggcat
     8101 cccacaggga gagcttgtag tgctgcccgc gcaggtggtc gccgcgcgtc ccgttcagcc
     8161 acgaggcgcg cggtggatcg tccacccacg ggtgccggat gccgatgtgg ttgttgacgt
     8221 ggtcgaagat gaccttc
//
LOCUS       DBNHQZ010000327         4877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1722477, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000327 DBNHQZ010000000
VERSION     DBNHQZ010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1722477"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC16YU_08730"
     CDS             <1..358
                     /locus_tag="AC16YU_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl phosphate translocase family
                     protein"
                     /protein_id="HIR6065349.1"
                     /translation="SAPTRGALDALDLRYIAVFAAGAGVGLAAFAILMNWLLARHHDA
                     TMAVLVGLMAGSLRALWPWQSAGAPRLADGAEPVLPVVAWAVVALGLSAALAWAEIRR
                     QRRSREFSAESVTAGA"
     gene            complement(342..1937)
                     /locus_tag="AC16YU_08735"
     CDS             complement(342..1937)
                     /locus_tag="AC16YU_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR6065350.1"
                     /translation="MKGRSDAPEYTYAPASPAVRLAALLLVAAAAAPAAGQVEPGAEC
                     IAGSAGPYACDGIDLRSRVSVAGLGGSDGVRLNDLWGWKDPDTGRLYALVGRADGTAF
                     VDVTDPDAPVYLGSLPSSGEWGASAWRDVKVYGHHAYVVADGVGEHGLQVFDLFDLRG
                     VEAPRTFQEAGNYAGFASAHNLFVNEETGFAYVCGASESAVPGGNACQGGLVMLDLSD
                     PVSPTHAGCFNHEGTGRAGTGYVHDVQCVVYHGPDTEHAGREICFGANETALSISDVT
                     DKAAPVALGLGTYPNTGYTHQGWLSEDHRWFFLGDELDELNGGAETTRTLVFDVSDLD
                     DPLPPIEYFSSRTSVDHNMYVTGGRLWQANYTDGVILLDVRNPAAPRELGRFDTTPGV
                     SSSVWNGAWSVYPFFGEDRVLASSRDEGLFVLDAGDAVLAATEGAPADLPGAIELLSA
                     FPNPFSASTVVEVRFASAGAARIDLVDVLGRVRSVIHEGPVVVGQAVSLRIEGDGLEA
                     GTYYVRIAGPEGGFRSIPVTRLP"
     gene            1953..2729
                     /locus_tag="AC16YU_08740"
     CDS             1953..2729
                     /locus_tag="AC16YU_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6065351.1"
                     /translation="MVLEDRTVLVTGASSGIGEACAHAFARAGARLLLCARREERLRP
                     VAFTLHSRYGVPVHHFELDVRDPRAVEQAFDTLPGGWRAVDVLVNNAGKAKGRAPVHE
                     ALEEHLEEMIDTNVKGLLYVTRAVLPGMLERGSGHVINIGSSAGRWIYPGGTVYCATK
                     AAVYAISEGLKMDLHGTGIRVSTVDPGLVETEFSLVRFDGDEAAAGEVYADTTPLTAE
                     DVAESVLFCATRPGHVNVSQVLMLPTDQSSSSMVHRRPSQ"
     gene            2917..4584
                     /locus_tag="AC16YU_08745"
     CDS             2917..4584
                     /locus_tag="AC16YU_08745"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6065352.1"
                     /translation="MRPTIILLAAWLAACSPPPQFDAIIAGGTVYDGLGGPGIRADVG
                     IVGDSVAAVGDLSERSARERVDAAGLAVAPGFVNMLSWADESLIQDPRGLSDLLQGVT
                     LEVFGEGSSMGPMNPRMVQDLSASQNRIRYDVSWTTLGGYLSFLEERGVSPNVASFVG
                     AGTVREYVLGDLAREPTDDELSRMRDLVREAMEEGAVGVGSSLIYTPGLYASTDELVA
                     LAAVAAEYDGLYISHIRNESDLLEEAVEEHLRITREAGIRSEIYHLKAAGRENWPRLE
                     RVIARIEEARAEGLDVGADIYTYTAGATGLDAAMPPWVREGGFGAWRDRLQDPALRAR
                     AARDMRAEPDGWENLYRAAGAGGTLLVGFRQDSLRHLIGRTLADLALERGVSPEEAAM
                     DLVVADSSRVSTIYFLMSEENVARKIATPWVTFGSDAAALAPEKPFTLSSAHPRAYGN
                     FARLLGRYVREEGLVPLGEAVHRLTDLPSRNLRIARRGRLAPGYFADVVVFDPVTIGD
                     VATYEEPHRLSTGVRDVWVNGVATVRDGAHTGATGGRFARRSTPRKP"
     gene            4599..>4877
                     /locus_tag="AC16YU_08750"
     CDS             4599..>4877
                     /locus_tag="AC16YU_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HIR6065353.1"
                     /translation="MNADCFRPIVLAAALVAAMVPHPASAQAGYSMIIEGGLVADGTG
                     RALFRADVGFRGDRIEAVGDLAGEPAAVRVDATGLVVAPGFIDIHSHAD"
BASE COUNT          676 a         1769 c         1713 g          719 t
ORIGIN      
        1 gtccgcgccg acgcgggggg cgctcgacgc gctcgacctc cgttacatcg ccgtgttcgc
       61 ggccggcgcc ggggtagggc tcgccgcgtt cgcgatcctg atgaactggc tcctggcccg
      121 ccaccacgac gccacgatgg ccgtcctcgt gggcctcatg gccggctctc tgcgggccct
      181 ctggccgtgg cagtcggcgg gagcgccgcg gctggccgac ggcgcggagc cggtcctgcc
      241 ggtcgtcgcc tgggcggtgg tcgcgctcgg cctgagcgcc gccctcgcgt gggcggagat
      301 ccggcgtcag cgacggagcc gggagttttc agccgaatcg gtcacggcag gcgcgtgacc
      361 ggaatgctcc ggaagccgcc ttccgggccg gcgatccgga cgtagtacgt tccggcctcg
      421 agtccgtcgc cctcgatccg cagcgacacc gcctgcccga cgacgaccgg gccctcgtgg
      481 atcacggacc ggacgcgacc cagcacgtcc accaggtcga tgcgcgccgc tccggccgac
      541 gcgaagcgga cctcgaccac cgtcgaggcc gagaacgggt tcggaaaggc cgaaaggagc
      601 tcgatcgcgc cgggcaggtc ggccggtgca ccctccgtag ccgccaggac ggcatcgccc
      661 gcgtcgagga cgaagaggcc ctcgtcccgc gaggaggcga gcacccggtc ctcgccgaag
      721 aacgggtaca ccgaccacgc gccgttccag acgctggagc tcacgcccgg cgtcgtgtcg
      781 aagcgcccca gctcccgcgg cgcagccgga ttccggacgt ccagcagaat cactccgtcc
      841 gtgtagttcg cctgccacag ccggccgccg gtcacgtaca tgttgtgatc gacgctcgtc
      901 cggctggaga agtactcgat cggcggcagg gggtcgtcga gatccgagac gtcgaacacc
      961 agcgtccgcg tcgtctcggc gccgccgttg agctcgtcca gctcgtcgcc caggaagaac
     1021 cagcggtggt cctcggacag ccacccctgg tgcgtgtacc ccgtgttcgg gtaggtgccg
     1081 agcccgaggg ccacgggagc cgccttgtcg gtcacgtccg agatgctcag ggccgtctcg
     1141 ttggcgccga agcagatctc gcgcccggcg tgctcggtgt ccgggccgtg gtagaccacg
     1201 cactgcacgt cgtgcacgta cccggtgccg gcgcggccgg tgccctcatg gttgaagcat
     1261 cctgcatgcg tggggctcac cggatccgac aggtccagca tgaccaggcc cccctggcac
     1321 gcgttgccac cgggcaccgc gctttccgac gccccgcaca cgtaggcgaa gcccgtctcc
     1381 tcgttcacga aaaggttgtg cgcgcttgcg aaacccgcgt agtttcccgc ctcctggaac
     1441 gtgcggggcg cctccacgcc gcgcaggtcg aagaggtcga atacctgcag gccgtgctcg
     1501 cccaccccgt ccgcaacgac gtacgcgtgg tgcccgtaca ccttgacgtc ccgccaggcg
     1561 ctcgcccccc actcgccgga gcttggcagg ctgccgaggt agacgggagc gtcgggatcg
     1621 gtcacgtcca cgaaggccgt gccgtccgcc cgcccgacca gcgcgtaaag ccggccggtg
     1681 tccgggtcct tccagcccca gagatcgttc aggcggacgc cgtccgagcc gccgagcccg
     1741 gccacgctca cgcgcgagcg gaggtcgatg ccgtcgcagg catacggccc cgccgacccg
     1801 gcaatgcact cggcccccgg ctcgacctgc ccggccgccg gcgccgcggc cgccgcgacg
     1861 agcagcaggg ccgccagccg cacggccggc gatgccgggg cgtacgtata ctccggggcg
     1921 tcggatcgtc ccttcacctg ccctcgaccg tcatggttct ggaagaccgg accgttctcg
     1981 tgaccggagc ctcgtccgga atcggagagg cctgcgccca cgcctttgcg cgggccggcg
     2041 cccgcctgct gctttgcgcc cggcgggagg aacggctccg gccggtcgcc ttcacactgc
     2101 attcacgata cggggttccc gtccaccact ttgagctcga cgtacgggat ccgcgcgccg
     2161 tggaacaggc cttcgacacg ctgcccggcg gctggcgcgc cgtcgacgtc ctggtcaaca
     2221 atgccggaaa ggccaagggg cgagcaccgg tccacgaggc gctcgaggag cacctcgagg
     2281 agatgatcga cacgaacgtc aaggggctgc tgtacgtgac gcgggccgtg ctccccggca
     2341 tgctcgaacg cggttccggg cacgtgatca acatcggttc ttcggccggg cggtggatct
     2401 acccgggcgg cacggtctac tgcgccacca aggccgccgt gtacgctatt tcggagggac
     2461 tcaagatgga cctgcacgga accggaatcc gcgtgagcac ggtggacccc gggctcgtcg
     2521 agaccgagtt cagcctggtc cggttcgacg gcgacgaggc ggccgcgggc gaggtctacg
     2581 cggacacgac gccgctcacc gcggaagacg tggccgagag cgtgctcttc tgcgcgacgc
     2641 gtcccgggca tgtgaacgtg agccaggtgc tgatgctgcc gaccgaccag tcgtcgtcct
     2701 cgatggtcca ccgccgcccg tcgcagtgag gcacccgttg cgtcctccgg cggcgaccgc
     2761 gatattgggc ccgtaaatcg gctgaacctc cctctcagcc cgttttcact gagcccggcc
     2821 gcgttcgctc gcgccgggct cgtcctttcc ggcggtcccg ggtgcgggct tgggcgacgt
     2881 gccgtaggtt gccggctcca tccgggaatc ccacccatgc gacccacgat catcctcctc
     2941 gcggcgtggc tggcagcctg ctccccccca ccgcagttcg acgcgatcat cgccggaggt
     3001 acggtgtacg acggtctggg gggaccgggc atccgcgccg atgtcggcat cgtgggcgac
     3061 tcggtggcgg cggtcgggga tctctcggag cggagcgcgc gcgagcgcgt ggacgcggcg
     3121 ggcctcgccg tcgctccggg cttcgtgaac atgctcagct gggccgacga atccctgatc
     3181 caggacccgc gcggcctgag cgacctgctc cagggcgtga cgctcgaggt cttcggcgag
     3241 ggctcgtcga tgggtcccat gaatccgcgg atggtccagg acctctccgc ctcgcagaac
     3301 cgcatccggt acgacgtgtc gtggacgacg ctcggcggat acctttcctt cctggaggag
     3361 cggggcgtct cgccgaacgt cgcctcgttc gtgggtgctg gcaccgtccg ggagtacgtc
     3421 ctgggcgacc tggcccgcga gcccaccgac gatgagctct cccggatgcg ggacctggtc
     3481 cgagaggcca tggaggaggg tgcggtcggc gttggatcgt ccctcatcta taccccgggc
     3541 ctgtacgcct cgaccgacga gctcgtcgcg ctcgccgccg tagccgccga atacgacggc
     3601 ctgtacatct cgcacatccg gaacgagagc gacctcctgg aggaggccgt cgaggagcac
     3661 ctccgcataa cgcgggaggc cggaatccgg tcggagatct accacctcaa ggcggcgggc
     3721 cgggagaact ggccacggct cgaacgggtc atcgcccgga tcgaggaggc gcgcgcggag
     3781 ggcctggacg ttggggccga catctatacg tatacggccg gggcgaccgg actcgacgcc
     3841 gcgatgcctc cgtgggtgcg cgagggcggt ttcggcgcct ggcgcgaccg gctccaggac
     3901 cccgctctga gggcccgcgc ggcccgcgac atgagggcgg agccggacgg gtgggagaac
     3961 ctgtaccggg cggccggagc cgggggcacg ctcctcgtcg gcttccggca ggactcgctc
     4021 cggcacctca tcggccggac gctcgccgac ctcgccctgg agcggggcgt ctcgcccgaa
     4081 gaggcggcca tggatctggt tgtagccgac tcgagccggg tctccacgat ctatttcctg
     4141 atgtcggaag agaacgtggc ccgcaagatc gccaccccct gggtgacgtt cggctcggat
     4201 gcggcggcgc tcgcgcccga gaagccgttc accctgtcga gcgcccaccc gcgcgcctac
     4261 ggcaacttcg cccggctcct ggggcgctac gtccgcgagg aaggcctcgt tccgctcggc
     4321 gaggccgtcc accgcctgac cgatctcccg tcccgcaacc tccggatcgc caggcggggc
     4381 cgcctcgcgc ccggctactt cgcggacgtc gtggtcttcg accccgtgac gatcggcgac
     4441 gtggccacgt acgaggagcc gcaccgcctg tcgacgggcg tgcgcgacgt gtgggtcaac
     4501 ggcgtggcga cggtccgcga cggcgcccac acgggtgcga cgggcgggcg gttcgcccgg
     4561 cgctccaccc cccggaaacc ctgacccgag gtccccggat gaacgccgat tgcttccgcc
     4621 cgatcgtcct ggcggccgcg cttgtcgccg ccatggtgcc gcaccccgcg tcggcccagg
     4681 ccggctattc catgatcatc gagggcggcc tcgtggcaga cgggacgggg cgggcgctgt
     4741 tccgggcgga cgtcggcttt cgcggcgacc gcatcgaggc ggtcggtgac ctggccggcg
     4801 agccggccgc agtgcgggtg gacgcgacgg ggctcgtcgt cgccccgggc ttcatcgaca
     4861 tccactccca tgcggac
//
LOCUS       DBNHQZ010000328         2985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1047347, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000328 DBNHQZ010000000
VERSION     DBNHQZ010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1047347"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16YU_08755"
     CDS             complement(<1..274)
                     /locus_tag="AC16YU_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065354.1"
                     /translation="MRRHGLIPLFLALTFLGLAGCGGVGSIIEKGDKAAARGDYWTAA
                     SAYLQALAKDSDNDEALAGLTSVAEPAYRQKADLAEGYEANDDLDHA"
     gene            496..>2985
                     /locus_tag="AC16YU_08760"
     CDS             496..>2985
                     /locus_tag="AC16YU_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR6065355.1"
                     /translation="MSFPESPSPAVDALPAVDEALPPPTPADHGSDDGLDYRLVCELA
                     TDTIFVHDPETGTILHANERACQALGYTLEEFRRDGVALVSDGNPPYDLAHAAEYLRM
                     AAAGQPQRFEWLAKNKSGERVWVEVRLHHVRMLGEPRVLAIVTVIEERKRAEAALRAS
                     EESYRSLFDSLSDLVYIQDLEGRFVNVNEAVVRAYGYTREEIIGRTPDFLAAPGRVDL
                     EETARRFRRAVEGERQQFQWWGLRRNGTIFPKEVVLSRSTYFGRDAVIAVARDISERV
                     EAENALRRSEEHFRRMIENASDLIAIMDPEGIIRYQSPSSRRMLGYEPEELVGTNAFS
                     YVSPEDVGPTADRWRQAMLTPEVPAMAEFRFRHRDGSYRVVETVAAVLSADRPEDGVV
                     VNSRDITARKEAERALLLQNALLEAAWESSIDGILVVGADGRIQSFNTRFAELWNLPD
                     DILDERSDEKALHAVLDGVVDPEGFLRRVNYLYDHPDVRSRDTITLRDGRVLDRYTSP
                     IVVASGEYQGRIWFFRDVTAEKRYAHDLDLARQEAELARAEEERARVDAEWARFEAET
                     AREEADRYAETLERELEYGRRVQQSLLPAVLPQPEGWDLAACFCPALQVAGDFYDAFE
                     LPGGHVGLMIADVSGKGVGAALFMALFQSQLRAAAERANGAMAPTGKAGDLGDIGDED
                     VLAQAVAETNDYVTRIRRNSRMFASVFFGVLDPESGILQYVNAGHEPPVVLRASGNRD
                     RLAPTGPATGLMPGAVFSVSRVDLGPGDRLVAFTDGISEARNMEGDFFGEDRLLDLLS
                     TDASTSSALLDSVERAVENFALGAEQSDDLTM"
BASE COUNT          463 a         1023 c         1025 g          474 t
ORIGIN      
        1 gggcgtgatc caggtcgtcg ttcgcctcgt aaccttcggc caggtcggcc ttctgccggt
       61 aggccggctc cgcgaccgag gtgagccccg cgagcgcctc atcgttgtcg gagtccttcg
      121 ccagcgcctg caggtaggcc gatgccgcgg tccagtagtc gccgcgcgcc gcggccttgt
      181 cccccttctc gatgatcgac cccacgccgc cgcagcccgc gagccccagg aacgtgagag
      241 caaggaacag gggtatcagt ccgtgtcgtc gcatggccgt gtccggctcg tcgatgagcg
      301 tatcacaggg gtaatcggac tcaaccggca tccataaagg ccgcgcgagt gacggagccg
      361 aaggggtcgc ccacggccgt tctccggtca gcctctcctc catttctcca cgattccgtt
      421 ccgaatcttc ccctgggtgc gtacgtattt cccgcacggc ggacaagggg ttcccccaca
      481 gcgtccgcga cccgcatgtc cttcccggaa tccccgagtc cggcggtgga tgcgctcccc
      541 gccgtggacg aggccctccc cccgccgacc cctgcagacc acggttccga cgacggcctc
      601 gactaccgtc tcgtctgcga gctcgcgacc gacaccatct tcgtgcacga cccggagacc
      661 ggcacgatcc tccacgcgaa tgaacgggcc tgccaggccc tcgggtacac gctcgaggag
      721 ttccgccggg acggcgtcgc gctcgtcagc gacggaaatc caccgtacga cctggctcac
      781 gcggcggaat acctgcgcat ggccgccgcg gggcagccgc agcgcttcga gtggctggcg
      841 aagaataagt cgggcgagcg cgtgtgggtg gaagtacggc tgcaccacgt caggatgctc
      901 ggcgaaccgc gcgtcctcgc gatcgtgacc gtcatcgagg aacggaagcg ggccgaagcg
      961 gccctgcgtg ccagcgaaga gagctatcgc agcctgttcg acagcctgtc ggacctggtg
     1021 tacatccagg acctcgaggg ccggttcgtc aacgtgaacg aggcggtggt ccgggcctat
     1081 gggtacacgc gggaagagat catcgggcgg actcccgact tcctggcggc gccgggtcgc
     1141 gtggacctcg aggagacggc tcgcagattc cgtcgggccg tggagggtga gcggcagcag
     1201 ttccagtggt ggggcctccg ccggaacggg accatcttcc cgaaggaagt cgtgctcagc
     1261 cggtccacgt acttcggccg ggacgcggtg atcgccgtcg cgcgcgacat ttcggagcgg
     1321 gtcgaggcgg agaacgccct gcgacgcagc gaggagcatt tccggcgcat gatcgagaac
     1381 gcgtcggacc tcattgcgat catggacccg gaaggcatca tccggtacca gagcccgtcg
     1441 tcccgccgca tgctggggta cgagccggaa gagctggtcg gcacgaacgc cttctcctac
     1501 gtctcgccgg aggatgtggg accgacggcc gaccggtggc gccaggcgat gctgacgccc
     1561 gaggtcccgg ccatggccga gttccgtttt cgccaccggg acgggtcgta ccgcgtcgtc
     1621 gagacggtgg ccgccgtgct gtcggcggat cggcccgagg acggcgtggt cgtgaactcg
     1681 cgcgacatca ccgcgcgcaa ggaggccgag cgtgccctgc tcctgcagaa cgcgctgctc
     1741 gaggcggcct gggagtcctc catcgacggg attctcgtcg tcggcgcgga cggccgcatc
     1801 cagtcgttca acacacggtt cgcggaactg tggaacctcc ctgacgacat cctcgacgag
     1861 cggtcggacg agaaggctct ccacgccgtc ctggacgggg tcgtcgatcc ggaagggttc
     1921 ctcagacggg tgaactatct ctacgaccat ccggacgtgc gctcccgtga cacgatcacg
     1981 ctcagggacg gccgggtgct ggatcgctac acgtcgccca tcgtcgtcgc gtccggtgag
     2041 taccaggggc gcatctggtt cttccgggac gtgacggccg agaagcgcta cgcgcacgac
     2101 ctggacctgg cccgacagga ggcggagctg gcccgggccg aggaggagcg ggcgcgggtc
     2161 gacgcggagt gggcccgatt cgaagccgag accgctcgtg aggaggccga ccgttatgcc
     2221 gagaccctgg agcgggagct cgagtacggc cggcgcgtgc agcagagcct cctcccggcc
     2281 gttctgccac agcccgaggg ctgggacctg gccgcctgct tctgccccgc cctccaggtg
     2341 gccggcgact tctacgacgc cttcgagctg cccggcgggc acgtgggcct gatgatcgcg
     2401 gacgtctcgg gaaagggcgt gggcgccgcg ctcttcatgg cgctcttcca gagccagctg
     2461 cgcgccgcgg ccgagcgcgc caacggggcc atggccccga ccggcaaggc cggcgactta
     2521 ggcgacatcg gcgacgagga cgtcctcgcg caggcggtgg ccgaaaccaa tgactacgtc
     2581 acgcgaatcc gccgcaattc gcggatgttc gcatccgtgt tcttcggcgt tctggatccg
     2641 gaatcgggca tccttcagta cgtgaacgcc ggccacgagc cccccgtcgt gctccgcgca
     2701 tccggcaatc gcgatcgact cgcccctacg ggccctgcga cgggcctgat gccgggcgcc
     2761 gtgttctcgg tctcacgggt cgatctcgga ccgggcgacc ggctggtcgc gttcaccgac
     2821 ggcatttccg aggcccggaa catggagggt gacttcttcg gtgaggaccg gctgcttgac
     2881 ctgctgtcca cggacgcttc cacctcgtcc gccctgctcg attccgtgga gcgggccgtc
     2941 gagaacttcg ccctgggagc cgagcagtcc gacgacctca cgatg
//
LOCUS       DBNHQZ010000329        11688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_637476, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000329 DBNHQZ010000000
VERSION     DBNHQZ010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_637476"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="AC16YU_08765"
     CDS             complement(<1..1852)
                     /locus_tag="AC16YU_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR6065356.1"
                     /translation="MKVYPPQVDEKLGFDAVRARLARTVRTGAAAERARALSPLPDAA
                     SVRTELGRVDALQGILAMGDPAPDIHFEPTREALARSAPAGAWLEPEELSAVRSLLAC
                     VRRVHAWLARTGSESEPVAALAAMLEPLPALEREIDTIVDAEGRVRDDASDTLVELRR
                     DEARTRDRLRGALQDALRRASSEGWAVEEQPTIRGGRMVIPLRAEARRKLDGIVHDVS
                     ATGQTVFVEPAACVELNNDLRTLEAEARREIVRLLTRLTNAVRAERARIGSNEEAAVY
                     MDLVISKARLANDLDARVPEDRGGGAIVLRAARNPELLLRIAATDVVPLDLELGTDCR
                     LLVISGPNAGGKSVALKTIGLFAMMLASGLPVPCAEGSRFDRFERLFVQMGDDQSVED
                     DLSTFSSQLRHVRAMLEGADGGTLVLLDEAGSGTDPEEGSALAQAALERLLDAGALIV
                     ATTHHANLKRFAHERERACNGMMAFDRERLTPTFRFEAGIPGASYAFEIADRIGVDRA
                     VTDRARELLGADRASLEQLLTDLQARNEALEERIREASAVAALAEERRHAMDERLARL
                     KEATSDIRERALEEAEAVIRDANAEVERAVREIREADADRTVTRAARERLE"
     gene            complement(1869..2879)
                     /locus_tag="AC16YU_08770"
     CDS             complement(1869..2879)
                     /locus_tag="AC16YU_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6065357.1"
                     /translation="MKLIIPMAGRGTRVRPHSHVTPKPLLPVRGKSMVERIVDTFNGV
                     LPRALDEGVFILAPDFGAGIRDELRAICDRHGMTAHFAVQDPPLGTAHAVDCAGEHLR
                     GEGIVVFADTLFYMEPGVELGDSDVVAWVKEVDDPSRFGVAVREGDRVVALVEKPQTL
                     ISKETLIGIYYVRDLARLAKAIRHLYDVDRKGPGGEYFLTDAFDVMLKDGIVFRTTTV
                     TDWLDCGTIPALQETTGIVLEREDDRPGGGSVADSVILPPVYVGPGATVVRSVVGPYV
                     SIEAGASVTDSVVRRSILFGEARVERSVLDGSLIGRHAEVTDHSGPVNVGDHSTVGRP
                     SK"
     gene            3018..3737
                     /locus_tag="AC16YU_08775"
     CDS             3018..3737
                     /locus_tag="AC16YU_08775"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR6065358.1"
                     /translation="MDPIERSEFLRGMSPSDIDAWHETRIRDLWRVFRIMGEFVEGFE
                     TLSRVGPCVSVFGSARIRPGSDDYELGRRVAARLVERGFGVITGGGPGIMEAANRGAR
                     EAGGVSVGLNINIPHEQSANPYIDGDALISFDFFFVRKVMFVKYAQGFIVLPGGFGTM
                     DELFEALTLIQTQKATRFPVVLMGTSHWKGLVSWIREVMLEGGKISEGDLDLFTLTDD
                     PHEAVDVVERFYRERAMTPNF"
     gene            3958..4809
                     /locus_tag="AC16YU_08780"
     CDS             3958..4809
                     /locus_tag="AC16YU_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065359.1"
                     /translation="MTGSRSHRMLSVIGAFAIALFLAMPAFGQEYKEAYNAALAAAKA
                     NNLTEALQKFTLAANGAKASGDTDVERRSNKVIAQIEYKLGLNATRDENYAVAMQHFE
                     NGIARYPSYSKNYLGRGLALKKLDRIDDAIAAFQQAISAGNAESDLQTARAAETAISE
                     HFVYLASSLLSRNGGKPSAADADQALAHLAKLSGLVEPDADVMYYTAEAFKAKGDFAQ
                     SVASAEQALALHRGSRTDKAKIYFVMGEALVGLGDTAGAKDAFQNAAVGTYRASAQHY
                     LETLGSR"
     gene            4889..5599
                     /locus_tag="AC16YU_08785"
     CDS             4889..5599
                     /locus_tag="AC16YU_08785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6065360.1"
                     /translation="MHAISIDFWNTLVVASANGRERQARRLDRLSAACESMEHPRSRD
                     RILAAYRLAQDRFNGTWLGERRTPPADRFVQWIWESLDVSVDSEEHDRTVDCFRNVLL
                     EHPPELAPGAEDTLRLLVPRFPIVIISDTMLSPGSTIRLLLDRLGLLDHFAGFVFSDE
                     SGFAKPDPRAFEAAALATGTTTRHLLHVGDLRPTDVAGALATGATAVLFTGIHLDQSE
                     GPEPDMELHAWADLPALV"
     gene            5605..6363
                     /locus_tag="AC16YU_08790"
     CDS             5605..6363
                     /locus_tag="AC16YU_08790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR6065361.1"
                     /translation="MSEPYIHESARVGEGTRVGRFSVIGQDVVIGSGCQIGHHVVIHD
                     GVSIGDGVRIDDHAVIGKQPMRAPNSAVTSGAEQPPSRIGDSCLLGAGVVVYAGCTLG
                     RKVLVADLATIREDVRVGDFTIVGRGAAIENKCTIGRYCKLETNSYVTAYSELEDRVF
                     VAPGVLTSNDNYIGRTEQRFHHFKGVTVRRGGRLGVGSVILPGREVGADAVVGAGAVL
                     TVDAEAETVYTGVPARPRREVAEEQKLANQGWID"
     gene            6411..7571
                     /locus_tag="AC16YU_08795"
     CDS             6411..7571
                     /locus_tag="AC16YU_08795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6065362.1"
                     /translation="MNLQMVDLRGQYLRIRDDVDAAMQAVIDTTAFIRGPAVGEFESA
                     LADYLGARYVHGVANGTDALQIALMALGVGPGDEVITPSFTFVATAEAAALLGAVPVF
                     ADIDPKTFNIDPAAVEALVTPRTRAIVPVHLFGQPAAMDAILDIGRRHGIPVIEDNAQ
                     GVGSTWRGRMTGTLGTIGTLSFFPSKNLGCYGDGGAVITMDEELYERMKLIANHGSKR
                     KYRNERVGVNSRLDTLQAAVLQAKLPHLDRFTADRREAADRYDAALGGLPGIEIPWRD
                     PDGMHVFHQYTIRVTSGGGSDGGAARDALAARLKEARIPHAVYYPVPLHRLPVFAPGG
                     EAECRHDDMVHTDRAADEVLSLPMHTELTREQIAYITEAVAAFAGERAEAGA"
     gene            7592..8635
                     /locus_tag="AC16YU_08800"
     CDS             7592..8635
                     /locus_tag="AC16YU_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065363.1"
                     /translation="MTAPGTAPHAEDRIGLAQVGIGYWGRNLLRNFASLPGAAMRVAC
                     DAREDVLAGIARQNPGIRTTTRFEEALSSDDVAAVVVATETPLHYAMARQALVAGKHV
                     FVEKPMAQTVEEAEELVSLADERGLVLMVGHLLLYHPAFRHVEDLIRAGELGEVYYLY
                     SQRVNLGIIRQKENAFESLAPHDLSVALQFLDARPESVAASGRDYLQPGIEDVVFATV
                     HFEGGRVAHLHTSWLDPHKIRKVTVVGSRRMAVIDDVESTEKVRLYDKGVDIGEAGYA
                     DFARAMTLRSGDIHIPRIPSTEPLGLECAHFVDCVRTGRTPRTDGRNGLSVVRMLEAA
                     RRSMAAGGIRVDV"
     gene            complement(8668..11259)
                     /locus_tag="AC16YU_08805"
     CDS             complement(8668..11259)
                     /locus_tag="AC16YU_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6065364.1"
                     /translation="MSTSDPISNRARRAPSPGRRPTAGTAGEPDQELDGFLDEAGREL
                     RKKGVDAQPAPPRRGGARKAAAPDADPAPPRKLPKKLLRSVKDDVVLTLLETGDVSPA
                     QVSRAQDRAKSNPGVPLWRGLLTARGVDRTVVLETAAQILDHPRVTVIDEQATLNLFD
                     SLKESHSAYWMGAIATRGALPVDSDLDASTGRLRVTFATSDPSDPELEGMAGKLGVTP
                     EFVYAPASVMDPLLETLVGDRPPPEEDPDEPEPGDEPTDAEEDDAGPLDPGAEHDRVT
                     TDLLRDGLVTRAQVAEALSRLDGGSARGGVWRMLASMDGVDGEKVYAHVAAAHSFRSE
                     KIKAGRPDTDFSHLILETMTPEQRRRMIELNLLPFDYEIDDRSGDPTLVFVTHDPTRA
                     EAHHLLGRLNAGRFELRYAPEKSVARVLSRLGAGATEPAVPRPAAVRTYDIAPTLEDA
                     PKPVAERRRTSAEPDKAIDALFEQLLVEAVRAGASDIHMYPDAGRHLEIHFRVDGRLR
                     PFKREGQLSADRFMASVREKAGIQADPERVGPMEGHLQRRIEDAPVRFRVSMLPMTTG
                     NGGPRTESIVIRIMDSRSVPTDLRRLGHGQDTRKKIERAVAKSQGMVITTGPAGSGRS
                     TTMAAALHTVLSPEVSTLSIEDPVDYVRDGVRHVQLDRRTDLEGALRAVLRHDPDVVL
                     VGEIRDRVSAGLAVKLANTGHLVFSILQAPDAISAVSALLRLGVEPFQVAYAVNLIVA
                     QRLVREVCAECSVTDDRRDPVLMRRLGFSDKEILSEAIRRASPGSTCSQCAGRGYRGR
                     RVVSEALYFSTPVRHLVTEAGSALDEETLRSVAAEEGMTTLAESYRALVLEGATTIEE
                     VMRVMTV"
     gene            complement(11279..>11688)
                     /locus_tag="AC16YU_08810"
     CDS             complement(11279..>11688)
                     /locus_tag="AC16YU_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6065365.1"
                     /translation="RVFSVLYSGSGDNINVIKIAAEACGNTYMEHQIKTVTVPMMLAQ
                     GTDLVKSMEASGVFTSMALARFRSGNETGNVRNAAQQMADYYENETTLKLRATVEGIQ
                     TVVAIVITLAIMALTVISSEIALISPQATDFMK"
BASE COUNT         1758 a         4182 c         3954 g         1794 t
ORIGIN      
        1 cctcgagccg ctcgcgcgcc gcccgggtca ccgtccggtc cgcgtcggcc tcccggatct
       61 cgcgcacggc ccgctccacc tcggcattcg cgtcccggat cacggcctcc gcctcctcga
      121 gggcccgttc ccggatgtcg gacgtcgcct ccttcagccg ggcgagccgc tcgtccatgg
      181 cgtgccggcg ctcctcggca agcgcggcca ctgccgaggc ctcgcggatc cgctcttcca
      241 gggcctcgtt gcgggcctgc aggtccgtga gaagctgttc gagcgaggcc cggtccgcgc
      301 ccaggagctc acgcgcccgg tcggtgaccg cgcggtccac gccgatgcgg tcggcgatct
      361 cgaacgcgta cgacgcgccc ggaatgcccg cctcgaaccg gaacgtcggc gtgagccgct
      421 cccggtcgaa ggccatcatg ccgttgcagg cccgctcccg ctcgtgcgcg aaccgcttga
      481 ggttcgcgtg gtgggtggtc gccacgatca gcgcgcccgc gtcgagcagc cgctccaggg
      541 cggcctgcgc gagcgccgat ccctcttcgg gatccgtgcc ggaacccgcc tcgtcaagga
      601 ggaccagggt ccctccgtcc gccccctcga gcatcgcccg cacgtggcgc agttgggagc
      661 tgaaggtcga caggtcgtcc tcgaccgact ggtcatcgcc catctgcacg aagagccgct
      721 cgaaccggtc gaagcggctg ccctcggcgc atggcaccgg aaggccactc gcgagcatca
      781 tggcgaacag cccgatcgtc ttgagcgcca ccgacttgcc gcctgcgttg ggaccgctta
      841 tcacgagcag ccggcagtca gtgccgagtt ccaggtccag gggcaccacg tcggtggccg
      901 cgatccggag caggagctcg ggattccggg ccgcgcgcag cacgatggcc cctccccccc
      961 ggtcctccgg cacccgcgcg tcgagatcgt tcgcgaggcg cgccttcgag atgacgaggt
     1021 ccatgtacac ggccgcctcc tcgttcgaac cgatgcgcgc ccgctcggcg cgaacggcgt
     1081 tcgtgaggcg tgtgagcagg cggacgatct cgcggcgggc ctcggcctcg agcgtccgga
     1141 ggtcgttgtt cagctccacg cacgcggccg gctccacgaa gacggtctgg ccggtggccg
     1201 acacgtcgtg gacgatccca tcgagcttgc gccgggcctc cgcgcgaagc gggatgacca
     1261 tgcggccgcc gcggatggtc ggctgctcct ccaccgccca gccctcggag ctcgcgcggc
     1321 gcagcgcgtc ctgcagtgcg ccgcgcaggc ggtcccgcgt gcgcgcctcg tcccgccgga
     1381 gctccacgag cgtgtccgag gcgtcgtcgc gaacccggcc ctccgcgtca acgatcgtgt
     1441 cgatctcccg ctccagggcg ggcagcggtt cgagcatcgc cgcgagcgcc gcaacgggct
     1501 ccgattcgga tcccgtccgc gccagccacg cgtgcacgcg ccggacgcac gccaggaggc
     1561 tccgcacggc cgaaagctcc tcgggctcga gccacgcccc ggccggcgcc gagcgggcca
     1621 gggcctctcg ggtgggctcg aagtggatgt ccggcgccgg gtcgcccatc gccaggatcc
     1681 cctgcagggc atccacgcgc ccgagctccg tccgcacgct tgcggcgtcc gggagcggcg
     1741 agagcgccct cgcccgctcg gccgcggccc cggtccgaac ggtgcgggcg aggcgagcgc
     1801 gcaccgcgtc gaagcccagc ttctcgtcga cctgcggggg gtagaccttc atgggcccgg
     1861 acgacgtctc atttcgacgg ccgaccgacc gtcgagtggt ccccgacgtt caccggcccc
     1921 gagtgatccg tgacctccgc gtggcgcccg atcagcgatc cgtccaggac ggaccgctcg
     1981 acgcgggcct ctccgaacag gatggagcgg cgcaccacgg agtccgtgac ggacgcaccc
     2041 gcctcgatcg agacgtacgg cccgacgacc gacctgacga ccgtcgcgcc cggccccacg
     2101 tacactggag gcaggatcac ggagtccgcc acggagccgc cgcccggccg gtcgtcctcg
     2161 cgctcgagca cgatgccggt cgtctcctgc agggccggaa tggtgccgca gtccagccag
     2221 tcggtcaccg tcgtggtccg aaacacgatg ccgtccttga gcatgacgtc gaaagcgtcg
     2281 gtcaggaagt actcgccccc gggacccttg cggtcgacgt catacaggtg ccggatcgcc
     2341 ttcgccaggc gggccaggtc gcgcacgtag tagatgccga tcagggtctc cttcgagatc
     2401 agcgtctgcg gcttttccac gagcgcgacg acccggtccc cctcgcgcac ggccacgccg
     2461 aaccggctcg ggtcgtccac ctccttgacc caggcgacga cgtccgagtc gccgagctcc
     2521 acgcccggct ccatgtagaa cagcgtgtcg gcaaagacca cgatgccctc gccacggagg
     2581 tgctcgccgg cgcagtcgac ggcgtgcgcc gttcccagcg ggggatcctg cacggcgaag
     2641 tgggccgtca tcccgtggcg gtcgcagatc gcgcgcagct cgtcgcgtat tccggcgccg
     2701 aagtcggggg ccaggatgaa cacgccctcg tcgagcgcgc gcggcagcac gccattgaac
     2761 gtgtccacga tgcgctcgac catgctcttc ccgcggacgg gcaggagcgg cttgggcgtg
     2821 acgtgcgaat ggggccgtac gcgggtcccg cgtccggcca tcgggatgat cagcttcatg
     2881 gagtcgtggg cggagtctcc cgcaagatag accgcgccag ccggccttgg gagcgccctt
     2941 gcgccaagac cgccccggcc gggcgggcga cgaagccggg cgtttcgtat cgtcacggcc
     3001 gaaccgaccg tgaaaccatg gatccgatcg aacgctccga attcctgcgc gggatgagcc
     3061 cgtccgatat cgacgcctgg cacgagaccc gcatccgcga cctgtggcgg gtcttcagga
     3121 tcatgggcga attcgtcgag gggttcgaga cgctctcgcg cgtgggccct tgtgtctccg
     3181 tgttcggctc agcgcggatc cgtccgggct cggacgacta cgagctggga cggcgcgtgg
     3241 cggcccggct cgtggagcgg ggcttcgggg tcatcaccgg gggaggcccg gggatcatgg
     3301 aagcggcgaa ccgcggagcg cgggaggccg ggggcgtgtc ggtgggcctg aacatcaaca
     3361 tcccgcacga gcagtcggcc aatccgtaca tcgacgggga cgccctgatc agcttcgact
     3421 tcttcttcgt ccgcaaggtg atgttcgtca agtatgccca ggggttcatc gtgctgccgg
     3481 gcggcttcgg cacgatggac gagctcttcg aggccctcac gctcatccag acccagaagg
     3541 ccacgcgctt cccggtcgtg ctcatgggca cctcccactg gaagggcctg gtgtcctgga
     3601 tccgggaggt catgctcgag ggcgggaaga tctccgaggg tgacctggac ctgttcacgc
     3661 tgacggacga tccgcacgag gcggtcgacg tggtcgagcg cttctaccgc gagcgcgcga
     3721 tgacgcccaa cttctgacgc gcagcggaaa ccggctcccc gggccggtac gtaaagacag
     3781 tccccacacg gttttacccc gcagccagga aacctcgacc ccgggtgccg ggttgttatg
     3841 aatcatgtcc ggcccggcct gcatgcgtgt cgtcgggccg acacggcggc ggagaaccgg
     3901 ttcacgccgt cttcgtattg cccccttcgc attatccaac cctctccctc aaccgacatg
     3961 accggctcac gctcacaccg gatgctctcc gtcatcggcg cattcgcgat cgcgctcttc
     4021 ctggccatgc cggccttcgg tcaggagtac aaggaggcgt acaacgctgc ccttgccgct
     4081 gccaaggcca acaacctgac cgaagccctg cagaagttca cgctcgccgc caacggcgcc
     4141 aaggcctccg gcgacacgga cgtggagcgt cgctccaaca aggtgatcgc ccagatcgag
     4201 tacaagctgg gtctgaatgc gacgcgtgac gagaattacg cggttgcgat gcagcacttc
     4261 gagaacggaa tcgcgcgcta tccctcctac tccaagaact acctcggacg cggcctcgcg
     4321 ctcaagaagc tcgatcgcat cgatgacgcg atcgcggcct tccagcaggc gatatcggcc
     4381 ggcaacgccg agagcgacct gcagacggcc cgcgccgccg agacggcgat cagcgagcac
     4441 ttcgtgtacc tggcgtccag cctgctcagc cggaacggcg gcaagccctc ggcggcggac
     4501 gcggaccagg ccctcgcgca cctggcgaag ctctccgggc tcgtggagcc cgatgccgac
     4561 gtgatgtact acacggcgga ggcgttcaag gccaaggggg acttcgccca atccgtcgcc
     4621 tcggccgaac aggccctggc gctgcatcgc ggcagccgga ccgacaaggc gaagatctac
     4681 ttcgtcatgg gcgaggcgct cgtcgggctc ggcgacacgg ccggcgcgaa ggatgccttc
     4741 cagaacgcgg cggtggggac ctaccgggcc tccgcccagc attacctgga gacgctcgga
     4801 tcccgctgat ccggccccga tttcaccgaa aagcccgcgg ctcgacctga tccgcgggct
     4861 tttcgttttt gtatcctgcg tcatgcccgt gcacgcgatc tcaatcgatt tctggaacac
     4921 cctcgtcgtc gcatcggcca acggccggga gcggcaggcg aggcgtctgg accgtctttc
     4981 ggccgcgtgc gagagcatgg agcatccccg gtcccgggac cggatcctgg cggcctatcg
     5041 cctggcccag gaccggttca acggcacctg gctcggcgag cgccggacgc ctccggccga
     5101 ccgtttcgta cagtggatct gggagtcgct ggacgtgtcg gtggacagcg aggagcacga
     5161 ccggaccgtg gactgcttcc gtaacgtgct gctcgagcat ccgcccgagc tcgctcccgg
     5221 tgcggaggac acgctccggc tcctcgtccc ccggtttccg atcgtgatca tctcggacac
     5281 gatgctgtcc ccgggctcga cgatccggct gcttctcgac aggctgggac tgctggacca
     5341 cttcgccggc ttcgtattct ccgacgagtc cggattcgcc aagccggatc cccgggcgtt
     5401 cgaggcggcg gccctggcga caggcacgac gacccggcat ctcctgcacg tcggggatct
     5461 gcgaccgacc gatgtcgccg gggcgctcgc caccggcgct acggccgtcc tcttcaccgg
     5521 catccacctc gaccaatccg aaggtcccga gcccgacatg gaactccacg cctgggccga
     5581 ccttcccgca ctcgtttgac ccccatgtcg gaaccctata tccacgagag cgcccgagtc
     5641 ggcgaaggca cccgggtcgg gcgcttctcg gtcatcggac aggacgtcgt gatcggctcg
     5701 ggctgccaga tcggccacca cgtggtcatc cacgacggtg tgtcgatcgg cgacggagtc
     5761 cgcatcgacg accacgcggt gatcggcaag cagccgatgc gcgcgccgaa ctccgccgtg
     5821 acgagcggcg ccgaacagcc cccctcgcgc ataggcgact cgtgcctgct cggtgccggc
     5881 gtcgtggtct acgccggctg caccctggga cggaaagtcc tcgtggccga cctcgccacg
     5941 atccgcgagg acgtgcgggt gggcgatttc acgatcgtcg ggcgaggcgc cgcgatcgag
     6001 aacaagtgca cgatcgggcg atactgcaag ctcgagacca acagttacgt caccgcgtac
     6061 tcggagctcg aggaccgcgt cttcgtggcg ccgggcgtgc tgacctcgaa cgacaactac
     6121 atcggccgga ccgagcagcg gttccatcac ttcaagggag tgacggtgcg tcgcggcggc
     6181 cgcctgggcg tcggcagcgt gatcctcccc ggccgcgagg tcggggcgga cgcggtcgtg
     6241 ggcgccggcg cggtactgac ggtcgacgcc gaagccgaga cggtgtacac cggcgtcccg
     6301 gctcgccccc gcagggaggt cgcggaagag cagaagctcg ccaaccaggg ctggatcgac
     6361 tgacgccccc gaatcccttt ccacagcacg cccgggcaga ccggagtccc atgaatctcc
     6421 agatggttga ccttcgcgga cagtatctcc gcatcaggga cgatgtagat gcggccatgc
     6481 aggccgtcat cgacaccacc gcgttcatcc gcggccccgc ggtgggcgag ttcgagtcgg
     6541 cgctcgcgga ctacctcgga gcgcgctacg tgcacggcgt ggcgaacggc acggacgccc
     6601 tgcagatcgc gctgatggcg ctcggggtgg gcccggggga cgaggtgatc acgccgagct
     6661 tcaccttcgt ggccaccgcc gaggcggccg ccctgctggg cgccgtgccc gtcttcgcgg
     6721 acatcgatcc gaagacgttc aacatcgatc cggccgccgt cgaggctctc gtgacgccgc
     6781 gcacacgggc catcgttccg gttcacctct ttggacagcc ggccgcgatg gacgcgatcc
     6841 ttgacatcgg ccggcgccac ggcatcccgg tgatcgagga caacgcccag ggcgtcgggt
     6901 cgacctggcg cggccgcatg accggcacgc tcggcacgat cggcaccctg tcgttcttcc
     6961 cgtcgaagaa cctcggctgc tacggggacg gcggcgccgt gatcaccatg gacgaggagc
     7021 tgtacgagcg catgaagctc atcgccaacc acggctccaa gcgcaagtac cgcaacgaga
     7081 gggtgggcgt gaactcgcgc ctcgataccc tgcaggcggc cgtcctccag gccaagctgc
     7141 cccacctgga ccgcttcacc gcggaccggc gcgaggccgc cgatcggtac gatgccgccc
     7201 tcggcggact gccgggcatc gagatcccgt ggcgcgaccc cgacgggatg cacgtcttcc
     7261 accagtacac gatacgggtc acctccggcg gcgggtcgga tggaggagcc gcccgtgacg
     7321 ccctggcggc ccgcctgaag gaggcacgga ttccgcacgc cgtctactac ccggtcccgc
     7381 tgcaccgcct cccggttttc gcacctggcg gcgaggccga gtgccggcac gacgacatgg
     7441 tccacaccga ccgggcggcg gacgaggtgc tgtcgctccc catgcacacg gagctgacac
     7501 gggagcagat cgcctacatc accgaggccg tcgcggcgtt cgccggcgag cgggcggagg
     7561 cgggcgcgtg atgggcactt ccgaacgagc cgtgacggcg cccgggacgg cgccgcacgc
     7621 cgaagaccgc attgggctcg cccaggtcgg catcggctac tgggggcgca acctgctccg
     7681 caacttcgcc tcgctcccag gggccgcgat gcgcgtggcc tgcgacgccc gcgaggatgt
     7741 cctggccggc atcgcgaggc aaaatccggg catccggacc acgacccggt tcgaggaggc
     7801 tctgtcctcg gacgacgtcg ctgccgtggt cgtcgcgacc gagaccccgc tgcattacgc
     7861 gatggcccgg caggcgctcg tggcgggcaa gcacgtcttc gtggagaagc cgatggcgca
     7921 gaccgtcgag gaggccgagg agctcgtatc cctcgcggat gagcggggcc tggtcctcat
     7981 ggtgggtcac cttctgctct accaccccgc attccgccac gtcgaggacc tgatccgggc
     8041 cggcgaactg ggtgaggtgt actacctcta cagccagaga gtcaacctcg gcatcatccg
     8101 gcagaaggag aacgcgttcg agtcgctggc cccgcacgac ctgtccgtcg cgctgcagtt
     8161 cctggacgcc cgccccgagt ccgttgccgc gtcgggccgc gactacctgc agcccggcat
     8221 cgaggacgtg gtgttcgcga cggtgcattt cgagggcgga cgcgtcgccc acctgcacac
     8281 gtcgtggctg gacccgcaca agatccgcaa ggtgacggtc gtcggcagcc gccgcatggc
     8341 cgtcatcgac gacgtggagt ccaccgagaa ggtccggctc tatgacaagg gcgtggacat
     8401 cggagaggcg gggtacgcgg acttcgcccg ggccatgacc ctccggtccg gtgacatcca
     8461 cattccgagg atcccgtcga ccgaacccct cgggctcgag tgcgcccatt tcgtcgactg
     8521 cgtgcgcacc ggtcgcacgc cgcggaccga cggacggaac gggctctcgg tcgtccggat
     8581 gctcgaggcc gcgcggcgct ccatggccgc cggcgggatc cgcgtggacg tctgaccgcc
     8641 cggaaggccc cgcctcggcc cggcacctca gacggtcatg acgcgcatga cctcctcgat
     8701 ggtcgtcgcg ccctcgagca cgagcgcccg gtaggattcg gccaaggtcg tcatgccctc
     8761 ctcggccgcg acactccgga gggtctcctc gtcgagcgcg ctgccggcct cggtgaccag
     8821 gtgacggacc ggggtcgaga agtacagggc ttccgagacc acgcgccggc cgcggtatcc
     8881 cctgcccgcg cactggctgc acgtggaccc ggggctcgcc ctgcggatgg cctcggagag
     8941 aatctccttg tccgagaagc ccaggcgccg catgagcacg ggatcgcgac gatcgtccgt
     9001 gacgctgcac tcggcgcaca cctcgcggac cagccgctgg gcgacgatca ggttcacggc
     9061 gtaggcgacc tggaacggct ccacgcccag gcgcagcagg gcactcacgg ccgagatcgc
     9121 gtccggcgcc tgcaggatcg agaagacgag gtgcccggta ttcgccagct tgaccgcaag
     9181 cccggccgac acccggtccc tgatctcgcc caccaggacg acatccggat cgtggcgtag
     9241 cacggcccgc agcgcgccct cgaggtcggt ccgccggtcg agctgcacgt ggcgaacgcc
     9301 gtcccgcacg tagtccaccg ggtcctcgat gctcagggtg ctcacctcgg ggctgagcac
     9361 ggtatgcagg gccgccgcca tggttgtcga ccggcccgat ccggccgggc cggtcgtgat
     9421 gaccatgccc tgcgatttcg ctaccgcccg ctcgattttc ttcctcgtgt cctggccgtg
     9481 tccgaggcgc cgcaggtccg tcggcacgct cctgctgtcc atgatccgga tcacgatgct
     9541 ctcggtgcgc ggccctccgt ttccggtggt catcggaagc atgctcaccc ggaaccgcac
     9601 gggggcatcc tcgatcctcc gctgcaggtg cccctccatg ggaccgaccc gttccgggtc
     9661 cgcctggatg cccgccttct cgcggaccga cgccatgaag cgatcggcgg agagctggcc
     9721 ctcacgcttg aacggccgca gcctcccgtc caccctgaag tggatctcga ggtgcctgcc
     9781 ggcatccggg tacatgtgga tgtcggaggc gccggccctc acggcctcga ccagcagctg
     9841 ctcgaagagc gcgtcgatcg ccttgtccgg ttccgccgac gtcctgcggc gctccgcgac
     9901 aggcttcgga gcgtcttcga gggtgggtgc aatgtcgtag gtccgcacgg cggcggggcg
     9961 cggcacggcc ggctcggtgg ccccggcgcc gagccgcgag agcacgcggg ccacgctctt
    10021 ctcgggcgcg tagcgaagct cgaaacgccc ggcgttcagc cgcccgagca ggtggtgggc
    10081 ctcggcccgc gtagggtcgt gggtcacgaa aacgagggtc ggatcgcccg accggtcgtc
    10141 gatctcgtaa tcgaaaggca gcaggttcag ctcgatcatc cggcgacgct gctcgggagt
    10201 catcgtctcc aggatgaggt gggagaaatc ggtgtcggga cgccccgcct tgatcttctc
    10261 cgaacggaac gagtgggcgg cggcgacatg ggcgtagacc ttctcgccgt cgacaccgtc
    10321 catggaggcc agcatcctcc atacgccgcc ccgtgcggat ccgccgtcca ggcgggagag
    10381 cgcttccgcc acctgcgcac gggtcaccag tccgtcccga agcaggtccg tcgtcacccg
    10441 gtcgtgctcc gcgcccggat cgagcggccc tgcgtcgtcc tcctcggcgt cggtcggttc
    10501 gtcgcccggt tccggttcgt ccggatcctc ctccggcggc gggcggtcgc ccaccagcgt
    10561 ctcgaggagc gggtccatca cggatgcagg ggcgtacacg aattccggag tcacgcccag
    10621 cttcccggcc atgccttcga gttcgggatc ggacgggtcg gatgtggcga acgtcacccg
    10681 caggcgcccc gtcgaggcgt cgagatccga atccacgggc agtgcgccgc gcgtagcgat
    10741 cgcgcccatc cagtaggcgg agtgcgactc cttcagggaa tcgaagaggt tcagcgtcgc
    10801 ctgctcgtcg atgacggtca cgcgcgggtg atccaggatc tgggccgccg tctcgagcac
    10861 cacggtgcgg tccactcccc tcgcggtcag caggccacgc cagagcggaa cgccgggatt
    10921 cgacttcgcg cggtcctgcg cccgggagac ctgggccggc gagacgtcgc ccgtctccag
    10981 gagggtgagg acgacatcgt ccttgaccga ccggaggagc tttttcggga gcttccgcgg
    11041 cggggcggga tcggcatcgg gtgccgccgc cttccgggcg ccgccccgtc ggggcggcgc
    11101 cggctgcgca tccactccct ttttcctcag ctcacgcccc gcctcgtcca gaaaaccgtc
    11161 cagctcctgg tcgggctcac cggccgtgcc ggcggtgggc cggcggccgg gcgaaggagc
    11221 ccgccgtgcc cgattcgata tcggatcgga ggtggacacc gatggaccag ggtgttgcct
    11281 acttcatgaa gtccgtagcc tgcggcgaga tcagggcgat ctcggacgag atgacggtca
    11341 gcgccatgat cgcgagcgtg atcacgatcg ccaccacggt ctggatgccc tcgaccgtcg
    11401 cgcggagctt gagcgtcgtc tcgttctcgt agtagtcggc catctgctgg gccgcgttgc
    11461 gcacgttgcc cgtctcgttg ccgctgcgga agcgggcgag cgccatggac gtgaacacgc
    11521 cgctcgcttc catcgacttc acgaggtcgg taccctgcgc gagcatcatg ggcaccgtca
    11581 cggtcttgat ctggtgctcc atgtacgtgt tgccgcaggc ctcggccgcg atcttgatta
    11641 cgttgatgtt gtcgccggag cccgagtaca gcacggagaa cacgcggc
//
LOCUS       DBNHQZ010000330         3170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2806217, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000330 DBNHQZ010000000
VERSION     DBNHQZ010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2806217"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC16YU_08815"
     CDS             complement(<1..475)
                     /locus_tag="AC16YU_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HIR6065366.1"
                     /translation="MATTVRKRQSGRVKSGDPLVSRQRKREVLGLILMVIAVLVALAV
                     LTYHADDDVIASRFSLDSVLDPADEDVANALGPTGAVLAGALVPGFLGYCVVLLPVIL
                     FGWGWVWFRNRTPVYLPLVSGLVTLAALFLATFFGWFAVVGDSDLLVWSGAWGLGL"
     gene            complement(560..1300)
                     /locus_tag="AC16YU_08820"
     CDS             complement(560..1300)
                     /locus_tag="AC16YU_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR6065367.1"
                     /translation="MKVEVFLTGASVGPEDGRGTTAVVIDVLRTSTTVTAALANGARA
                     VVPVADMAEAARVAENLDPSTMLLGGERGGVTIDGYQLGNSPREYDAATVRGRTVVMV
                     TTNGTGAVVRCAAASEVVIGGLVNARAVVNHVLDRARDVTIVCAGWKNRVSLEDTLCA
                     GLLIDLMFPGHVPEPETDTAHIALAQYRYDRDGLAARIATCNHARRLVALGHGADIPI
                     CSAVDSIPVVPAYRDGRLVLAGVAESAR"
     gene            complement(1312..2430)
                     /gene="gcvT"
                     /locus_tag="AC16YU_08825"
     CDS             complement(1312..2430)
                     /gene="gcvT"
                     /locus_tag="AC16YU_08825"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6065368.1"
                     /translation="MSDTAVKHTPLHAEHVRLGARMVPFAGFDMPVYYGGIIEEHMAV
                     RGAAGIFDVSHMGEVMATGPHAEEFVQRLVSNDVTRLTDGRAMYTVMCRPDGGIVDDL
                     LVYRMGPSEYMLVVNAANIEGDVAWMRAHNPMGADLEDISDGTALVAVQGPRAFEIVG
                     RIAGRPLDDLPFYHFTVPAPGSFLGCRRAVLSHTGYTGEPGLEIYCEEEKALDVWTAV
                     MDAGADLGLVPAGLGARDTLRLESGFCLYGNDITTATNPLEAGLGWLTKLDKGDFVGR
                     DALVRAKEAGIGRRLVAFVLSERGIPRAGMEIVDASGGVIGAVTSGTQSPILGQGIGM
                     GYVPNDPAHTAPGSPIRIQVRGRVIEGTVRKPPLHKPS"
     gene            complement(2496..>3170)
                     /locus_tag="AC16YU_08830"
     CDS             complement(2496..>3170)
                     /locus_tag="AC16YU_08830"
                     /inference="COORDINATES: protein motif:HMM:NF042869.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR6065369.1"
                     /translation="YVVCPPERGAEDDPRFRALVALLEKTGARVLTMSAERHDEAAAA
                     VSHLPQLLAVALVGQLGDLADDGTSRHLAAGGFRDMTRIASSPYGVWRDILQANHGPI
                     LDAMARFAARFERLRGRLADDDLEALGDAFAEAADTRATVPARSKGFLRPLADVFVYT
                     PDEPGALHRITGALADGGVNIKDIELLKIREGTGGTFRLGVDGDRDAERAVEVLGAAG
                     FQAYRL"
BASE COUNT          485 a         1116 c         1133 g          436 t
ORIGIN      
        1 cgaggccgag accccaggca ccgctccaga ccaggaggtc cgagtcgccg actacggcga
       61 accatccgaa gaacgtggcc aggaacaggg cggcgagcgt gacgaggccg ctcacgagcg
      121 gcaggtacac cggcgtccgg ttacggaacc acacccagcc ccagccgaag aggatgaccg
      181 gcagcagcac cacgcaatac cccaggaatc ccggcaccag ggcgccggcc aggactgcgc
      241 cggtcggacc gagcgcgttc gcgacatcct cgtcggcggg atcgaggacg gagtccagcg
      301 agaagcggct ggcgatgacg tcgtcgtccg cgtggtaggt gagcacggcg agcgcgacca
      361 gcacggcgat caccatgagg atcagcccca ggacctcgcg cttccgctgc cgggagacga
      421 ggggatcgcc ggatttcacg cggccggact gccgcttgcg cacggttgta gccatgggcg
      481 aaaactacgg cagcggagag gggcgtcaaa aggacgcggg cgtcccgctc gcgcggatcg
      541 gtccggcccg tccggccggc taccgggcgc tctcggccac gcccgcgagc acgagtctgc
      601 cgtccctgta agccgggacg acggggatgg agtcgaccgc ggagcagata gggatgtccg
      661 caccgtggcc gagggccacg agccggcgcg cgtggttgca cgtggcgatg cgcgcggcga
      721 gtccgtcccg gtcgtagcgg tactgggcga gggcgatatg cgcggtgtcg gtttccggct
      781 ccggaacgtg gcccggaaac atgaggtcga tcaggagacc cgcgcacagc gtgtcctcga
      841 gcgagacccg gttcttccag cccgcgcaca cgatcgtcac gtcgcgtgcc cggtcgagca
      901 cgtggttcac gacggcgcgg gcgttcacca ggccgccgat caccacctcc gaggccgcgg
      961 cgcagcgcac gacggcgcca gtgccgttgg tcgtgaccat cacgacggtc cggccgcgaa
     1021 ccgtcgccgc gtcgtactcg cgcggagaat tgccaagctg gtatccgtcg atggtgacgc
     1081 ctccccgctc gccgccgagc agcatcgtcg acggatccag gttctcggcc acccgggccg
     1141 cctcggccat gtcggccacc gggaccacgg cccgggcccc gttcgcgagc gcggccgtca
     1201 ccgtggtgct ggtccggagc acgtcgatca ccacggccgt cgtgccgcgg ccatcctccg
     1261 ggccgacgga cgctccggta aggaaaacct cgaccttcat ggcgggagcg gtcagctcgg
     1321 cttgtggagc ggcggcttgc gcacggtacc ctcgatcacg cggccccgca cctggatccg
     1381 gatcgggctg cccggcgccg tgtgggccgg gtcgttgggg acgtacccca tgccgatgcc
     1441 ctggcccagg atcggggact gcgttccgct cgtcaccgcg ccgatcacgc cgccggacgc
     1501 gtcgacgatt tccattcccg cgcgcgggat gccgcgctcg gagagcacga acgccacgag
     1561 acgccggccg attccggcct ccttcgcacg gacaagggcg tcgcggccca cgaaatcgcc
     1621 cttgtcgagc ttcgtgagcc agccgagccc ggcctccagc ggattggtcg ccgtcgtgat
     1681 gtcgttgccg tacaggcaga agcccgactc gaggcgcagg gtgtcccgtg cgccgagacc
     1741 cgcgggcact aggcccaggt ccgcgccggc gtccatgacg gccgtccaca cgtcgagggc
     1801 cttctcctct tcgcagtata tctcgaggcc gggctcgccc gtgtagccgg tgtgcgagag
     1861 aacggcccgc cggcacccca ggaaggaccc cggggcgggc acggtgaagt gatagaacgg
     1921 caggtcgtcg agggggcggc cggcgatccg ccccacgatc tcgaacgccc ggggaccctg
     1981 cacggcgacc agggcggtcc cgtcggagat gtcctcgagg tcggcgccca tcgggttgtg
     2041 ggcgcgcatc cacgcgacgt ccccctcgat gttcgccgcg ttgacgacga gcatgtactc
     2101 ggacggcccc atccggtaga ccagcaggtc gtcgacgatg cccccgtcgg ggcggcacat
     2161 caccgtgtac atggcccgtc cgtccgtgag cctggtgacg tcgttcgaga ccaggcgctg
     2221 cacgaactcc tccgcgtgcg ggcccgtcgc catcacttct cccatgtggc tcacgtcgaa
     2281 gatcccggcg gcgccgcgga cggccatgtg ctcttcgatg atgccgccgt agtagaccgg
     2341 catgtcgaag ccggcgaacg gaaccatgcg ggcgcccagc cggacgtgct cggcgtggag
     2401 cggggtgtgc ttgaccgcgg tgtcggacat ggctgtgggc ggatggtgca ggtggaatga
     2461 taccgacggc cccgaaccgt cggcatgaat ttgcgctaca gacggtaggc ctggaagccg
     2521 gcggcaccca ggacctccac ggcccgctcc gcgtcgcggt ctccgtccac gcccaggcgg
     2581 aacgtgccgc cggtgccttc ccggatcttc aggagctcga tgtccttgat gttgacgccg
     2641 ccgtcggcca gggcccccgt gatgcggtgc agggcgcccg gctcgtcggg cgtgtacacg
     2701 aagacgtcgg ccagcgggcg gaggaagccc ttggaacgcg cggggacggt ggcccgtgtg
     2761 tccgccgcct ccgcgaacgc atcgcccagc gcctccaggt cgtcgtcggc cagcctaccc
     2821 cgcaaccgct cgaagcgggc ggcaaaccgg gccatggcgt cgaggatcgg gccgtgattc
     2881 gcctggagga tgtcccgcca cacgccgtac ggcgacgacg cgatccgggt catgtcgcga
     2941 aagccgccgg cggcgaggtg gcgcgacgtg ccgtcgtccg cgagatcgcc cagctggcca
     3001 acgagcgcga cggcgagcag ctggggcagg tggctcactg cggcggccgc ttcgtcgtgc
     3061 cgttcggccg acatggtcag gacccgtgcc cccgtcttct cgaggagcgc cacgagcgcg
     3121 cggaacctcg gatcgtcttc ggcaccgcgc tccggggggc acacgacgta
//
LOCUS       DBNHQZ010000331        13104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1318793, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000331 DBNHQZ010000000
VERSION     DBNHQZ010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1318793"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1514
                     /locus_tag="AC16YU_08835"
     CDS             171..1514
                     /locus_tag="AC16YU_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6065370.1"
                     /translation="MNRPRPQEYRIDLELTRRLSPERTTAYRTVSRVLPGFRFVDLYR
                     AVDTVLEDRTGVFRFATSQIEALQSILHSLPPAWSNRVMTWAPSVSWPVGPGEETLLP
                     EDQFWVAPSSSGWYRVVVRLKYDAGNRSSLLEVAALEPEEGDALVEAVLAVSGRDSIY
                     RNRTIDIAFRTSATGDYGDVEKLETLHVGFKPVETVADEDIVFDEDIRRILHRNVLDL
                     HERRELLAELGVPARRGVLLYGPPGTGKTFACRYLAGKLVNVTRIVVTGSALMQVGQV
                     FELARLLQPSVLFLEDVDLVFMDRQVNSFRSLLGDLLDQMDGLRPFEDIGFVLTTNAI
                     ENVEEAIRDRPGRISQCVRFGPPSPELRDRFLRRYLERYDWSRVDLDRLVGESEGVTQ
                     AFLKEWVHRSVQIASERIEAPGGELDLRTGDFDTALREMKAFSTGSTARIVGFRD"
     gene            1532..2296
                     /locus_tag="AC16YU_08840"
     CDS             1532..2296
                     /locus_tag="AC16YU_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065371.1"
                     /translation="MKSADRKPEQPTVHGPLFAPSGSDDLSSHRHRLLIGSMGFVLPL
                     SLWLLAAWRPTPGLPRWGILDSVSAYYHTGAVAAFVGVLTSLAMFLFTYQGYGNEHNL
                     RDRIAAVTSGIAALGVALFPADTVAGLPRPPWFEPWMPVVHYLSAAVLFGAFFVFSFF
                     LFPMSKPHRVKQLPTDKQVRNVVYRVCGVIILACMLWILVMEFGPQRSIFWPESVALG
                     AFSLSWLVKGRADWTVVSASKKAWHYGPGQLGGPAE"
     gene            2466..2825
                     /locus_tag="AC16YU_08845"
     CDS             2466..2825
                     /locus_tag="AC16YU_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR6065372.1"
                     /translation="MSSPGTPTRTSPPAPEVEVGVAEEEEVRAIHVPRYHVVLFDDDD
                     HSYEYVIEMLMALFGHPLRTAYDMASTVDRAGRVIVETTTKERAELKRDQIHAYGPDW
                     RIPHCVGPMAADVEPAE"
     gene            complement(2906..3466)
                     /locus_tag="AC16YU_08850"
     CDS             complement(2906..3466)
                     /locus_tag="AC16YU_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6065373.1"
                     /translation="MRILAAILLLAAACGAPVPPEAPAPAPEATLDPDFDPDARLAEL
                     GITLPAPGAPIASYVNAVRSGDLLFLAGKGPTRPDGTSVTGRLGDGLSVEEGYEAARL
                     TAIQHLAVLQAELGDLRRVRRVVKVTGMVNATADFTQHSAVINGYSDLMAAVFGDRGL
                     HARAAVGMASLPTGIAVEVEAVVEVE"
     gene            3633..5234
                     /locus_tag="AC16YU_08855"
     CDS             3633..5234
                     /locus_tag="AC16YU_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6065374.1"
                     /translation="MKRFTTSALFLLVAMSAAAQPWIRRPTGPDYPTGDKVIEAIWDE
                     GMNRSQAADLAQVLADVIGPRLTAAPEHAKGVEWAVGTLEAWGIPAEAEPYGTWRAWR
                     RGTSHVDLLEPRVRSLEAVMLGWSPGTGGPRQGGTVLLPEAEGPEAFRAWLPDARGKF
                     VLLTYAEPSCRPASSWTEHGLPGAADAFNEARTAAREAWTARIDAIGMERQEIIDALE
                     GAGALGFLTNYWTGGWGTDRIFPLTYSFQAMNRRAAAFNLSCEDYGLVFRLTENGQGA
                     VLRADADAEDLGEQPVHNVIGRIEGTELPGEVVLLSAHFDSWDGASGMTDNGTGSVVM
                     LETMRILKKLYPNPKRTIMIGLWGAEEQGLVGSRAFVADHPEIVDHLQVVFNQDNGTG
                     RIRSVSTQGLTRAGEFFARWFSQMPEILVGDIDLNVPGSPGGGGSDYAAFICAGAPAF
                     SLSSTSFDYGTATWHTNRDTFDKLSIEDVKRNATVTAMLTYLASEDETMPRDRRELGN
                     DPETGEPLEWPTCEDGARETTGRFR"
     gene            complement(5284..5919)
                     /locus_tag="AC16YU_08860"
     CDS             complement(5284..5919)
                     /locus_tag="AC16YU_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065375.1"
                     /translation="MCSHRRRLLAALVLAVLVPGPARAQFCFRDGAPPDCGVVGIVEF
                     GGLWAFENDLRQDNVNTGFWEVGAMRNRADGRTALGASLYASYDDTEYRNMLWAVKAR
                     GRYRLTRHLKLDVGAGPIVAARDLDEWVDVRLPGATFRATLNVHDWVGLTAGFDAYRM
                     RVSRELPPVARNRYLDEQWSWFVGAQANGYLGVLGGVAGAAMLVIVNKIRD"
     gene            complement(6042..7421)
                     /locus_tag="AC16YU_08865"
     CDS             complement(6042..7421)
                     /locus_tag="AC16YU_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065376.1"
                     /translation="MNRRKFLAAGTLAGVGYTLVPKAAARSAAGPAPALVRRGPSDAV
                     RLGFIGTGLRGRSHVDLALRRDDVAVTALCDIDPAALASAEKQLADAGRPKAAVYGDS
                     EYAFLDLLARSDVDAVVIATPWLWHTRMAVAAMRAGKYAGLEVSAANTLEECWDLVDT
                     HEETGVPMMILENVCYRRDVMAVLNMVRQGLFGELVHMECGYQHDLREVKFNDGTRPY
                     GGGVEFGEKAFSEARWRTWHSVHRNADVYPTHGLGPVGVMLDVNRGNRFVSLTSTASK
                     ARGLHDYIVKQAGEDHPNARVEFRLGDVITTVIRTQNGESIIVSHDTNLPRPYSLGFR
                     VQGTEGIWMDVNRSIYLEGKSPAHQWEDAQPYLDRYDHPLWKRHAERATGAGHGGMDF
                     FVLHAFIESVKAQGPTPLDAYDAAAWSAVTPLSEMSIASGSAPQLFPDFTRGLWMKRR
                     PVFALDDSY"
     gene            7592..9355
                     /locus_tag="AC16YU_08870"
     CDS             7592..9355
                     /locus_tag="AC16YU_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6065377.1"
                     /translation="MQLSTLDWAIMAAFFAVSLIIGVAVARRSGRDFSAFFLAGRSMP
                     WWFLGISMVATTFSTDTPNLVTDIVRSNGTYGNWTWWAFLLTGMLTVFLYARLWRRSG
                     VLTDVEFYELRYSGRIAAFLRGFRAAYLGIVFNVLIMASVTLAAIKIGSVMFGWSPLR
                     TVTVAALVTMTYSAIGGLRGVVLTDLLQFALAMIGSIGAAVYVLGLPEVGGLDGLLAN
                     GRVREAMDFLPDFATLGWEQVLPVFIVPLAVQWWAAYYPGSEPGGGGYVVQRMLSARN
                     ERQAAGATLLFNTLHYALRPWPWILVALASLVVFPDLASIAARFPHVPEQVVRNDLAY
                     PAMLTFLPAGLLGIVVTSLAAAYMSTVSTQVNWGSSIIVNDLYHRFLNREASERQQVW
                     AGRVATVVLMVTACGLALLLENALQVFNIILQIGAGTGLLYILRWFWWRINAQAELVA
                     MVASFGVALWFAFVSDHGLEGWAQLVIGVAVTTAAWVTVAFVTKPTDPRVLDAFYRKI
                     HPGGPGWKPVEARVGKPEGSSDLPYALVAALCGAVAIYGALFATGFYLYGRAGAGIGM
                     TVVSLAAAAGLAAVWRRLAFR"
     gene            complement(9399..10736)
                     /locus_tag="AC16YU_08875"
     CDS             complement(9399..10736)
                     /locus_tag="AC16YU_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HIR6065378.1"
                     /translation="MTTPDDILAASGMDPATAARHLEALGELDAAAGDDARERHRLLV
                     PGRIEILGKHTDYAGGSSLTCASARCIRVLAVPRDDGLVRIHEPDLGRYAALEAGVGA
                     SAAPGHWSNYAAAVIRRVAMNAPGSLQGADLWLSSDLPRAAGMSSSSAFIVAVFLGLD
                     AAGGLRRGGPLAAALPDADALAGYLASVENGSGFGPLAGERGVGTSGGSEDHVAILLS
                     RSGHIGRYRYAPFRREGSVEIPRHLGLVVGASGVTANKTGSARARYNRASDLAREAVA
                     AWTASTGEEAGHLGDLVERDPDAARRLAEALPAGEGTDALLRRVRHFGVEHAEVVPAA
                     WDALSRGDIWAFGAAVDRSQHAAEELLGNQVPETMHLARSARELGAVASSSFGAGFGG
                     AVWAMVPRADSDRFLEAWRNEYVAAFPRRASRARFFPEPTGPGALRLDGRPVW"
     gene            11139..11648
                     /locus_tag="AC16YU_08880"
     CDS             11139..11648
                     /locus_tag="AC16YU_08880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR6065379.1"
                     /translation="MIGPILFFVFAITAIAGALGMLLSRNPVSSALWLILTLFCIAGL
                     YLTLNATFISVVQVLVYAGAIMVLFVFVIMLLNLADLPRLKTVGWRRVIAFALGLGVL
                     AQLAYVTAAALDVLPAPVSLEAAAESGSAAVLARVLFTQYAFPLEVIGILLLVATIGA
                     VMLAKRRFV"
     gene            11675..11980
                     /gene="nuoK"
                     /locus_tag="AC16YU_08885"
     CDS             11675..11980
                     /gene="nuoK"
                     /locus_tag="AC16YU_08885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR6065380.1"
                     /translation="MEVTINWYLTLSAVLFVIGVLGVLLRRNAIVILMSVELMLNAVN
                     LTLVTFAQSMGDTAGQILVFFVMSVAAAEAAVGLAIVIAIFRNRLTVDVSQINLFRY"
     gene            12018..>13104
                     /locus_tag="AC16YU_08890"
     CDS             12018..>13104
                     /locus_tag="AC16YU_08890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR6065381.1"
                     /translation="MDIDPLVLLILLLPLAGAVLNGLVPLFVPSLRGRETLIGTLGTL
                     VVAVPFALTVALFLGFEGTTHITQVAPWISAGDFSVSFAYRIDALSLTMAMIVSGVGG
                     IIHLYSIGYMHGDPGFFRFFSYLNLFIFMMLNLVLGDNLLVLFLGWEGVGLCSYLLIG
                     FWYTDLKNSAAGNKAFIMNRVGDFAFLLAMFIFVRELGTLDFGTLLAEGPSMDPATVN
                     WAVLLLFIGATGKSAQIPLFTWLPDAMAGPTPVSALIHAATMVTSGLYMLARMSPVVL
                     AAPTIMAVIAIVGALTAIVAATIAITQNDIKKVLAYSTVSQLGYMCLAVGMGAWVSAM
                     FHVMSHAFFKACLFLGSGAVIEACHHNQ"
BASE COUNT         1815 a         4666 c         4511 g         2112 t
ORIGIN      
        1 cctccccggc cattccaccc cggcagatgg gtcaccctgt tgactcggcc ggccacggcg
       61 cgtaataacc ggaaatatcc gcccgctgcg acgccccgat ccttgtttcc cggggcgtgc
      121 cgctcgcggg ccgcacgctg aacctgattc ccggaggcgt catgttcgga atgaatcgcc
      181 ccagacccca ggaatacaga atcgacctgg aactcacccg gcgtctctcg cccgagcgga
      241 cgacggccta tcgcaccgtg tcacgtgtcc ttccggggtt ccggttcgtg gacctgtacc
      301 gtgccgtgga caccgttctg gaggacagga ccggcgtttt ccgctttgcc acctcgcaga
      361 tcgaggccct tcaaagcatc ctccacagcc tgcctccggc gtggtccaac cgggtcatga
      421 cctgggcacc cagcgtctcc tggcccgtgg gtcccggcga ggagacgctg ctgccggagg
      481 accagttctg ggtcgcaccc tcgagctccg ggtggtaccg cgtggtcgtg cggctcaagt
      541 acgacgcggg caatcggagc tcgctcctcg aggtggccgc cctggagccc gaggagggcg
      601 acgccctggt cgaagccgtc ctcgccgtta gcggccgcga ctcgatctac cgcaaccgga
      661 ccatcgacat cgccttccgg acgagcgcga ccggcgacta cggggacgtg gagaagctcg
      721 agaccctcca cgtcgggttc aagcccgtgg agacggtggc cgacgaggac atcgtgttcg
      781 acgaggacat ccggcgcatc cttcaccgga acgtcctgga ccttcacgag cggcgggagc
      841 tgctcgcgga actcggcgtg cccgcacggc gcggcgtgct gctttacggg ccgccgggca
      901 ccggcaagac cttcgcctgc cgctacctgg ccggcaagct cgtgaacgtc actcggatcg
      961 tggtcacggg cagcgccctc atgcaggtgg gccaggtctt cgagctcgcc cggctgctcc
     1021 aaccgtcggt cctcttcctg gaggacgtgg acctcgtgtt catggaccgg caggtgaact
     1081 cgttccgctc gctgctcggc gacctgctgg accagatgga cgggctgcgg cctttcgagg
     1141 acatcgggtt cgtgctcacg accaacgcca tcgagaacgt ggaggaggcc atccgcgacc
     1201 gccccggccg catcagccag tgcgtgcgct tcggcccgcc gagcccggaa ctcagggacc
     1261 gattcctgcg ccgctacctg gaacgctacg attggagtcg ggtcgacctg gaccgcctgg
     1321 tgggcgagtc ggaaggggtg acccaggcct tcctcaagga gtgggtgcac cggtcggtgc
     1381 agatcgcgtc ggagcgcatc gaggcgccgg gcggcgaact ggacctgcgg accggggact
     1441 tcgacactgc gctgcgcgag atgaaggcct tctcgacggg atccacggcg cgcatcgtcg
     1501 gattccgcga ctgaccaggc cctatccgcg catgaaatcg gccgaccgga agcccgaaca
     1561 gcccaccgtc cacggaccgc tcttcgctcc gtccggatcg gacgaccttt cgagccacag
     1621 gcatcggctg ctgatcggtt cgatgggctt cgtgctgccc ctgagcctgt ggcttctcgc
     1681 cgcctggagg cccacccctg gcctcccgcg ctggggcatc ctggactcgg tcagcgccta
     1741 ctaccacacg ggcgccgtgg cggctttcgt cggcgtgctg acctccctcg ccatgttcct
     1801 gttcacgtac caggggtacg gcaacgaaca caaccttcgg gatcggatcg ccgccgtcac
     1861 ctccggaatc gcggctctcg gcgttgcact gtttccggcc gacacggtcg ccgggcttcc
     1921 tcggcccccg tggttcgagc cctggatgcc cgtggtccac tacctgagcg ccgcggtgct
     1981 cttcggcgcc ttcttcgtct tctcgttctt cctcttcccg atgtcgaagc cccatcgggt
     2041 caagcagctg cccacggaca agcaggtccg caacgtcgtg taccgggtct gcggcgtgat
     2101 catcctggcc tgcatgctgt ggatactcgt catggaattt ggccctcagc ggtcgatctt
     2161 ctggcccgag agcgtggccc tcggggcctt ctccctgtcc tggctcgtga aggggcgcgc
     2221 cgactggacc gtggtatcgg cgtcgaagaa ggcctggcac tacgggcccg ggcaactggg
     2281 cgggccggcg gagtgaggat cttcggcggc cggcgcatga tgcaaagcgg tggcgtgtcg
     2341 ctccagtgcg ccaggggtag cctcgaggga tgcgtcgtcg ttacgttcct cccggaagca
     2401 gtgacaccct gttgcgcgcg caaacgatcg gacacgcctt ccatacgact ccggggcaac
     2461 gcatcatgtc ctctcccggc actccgaccc gaacgtcccc tcccgcgccc gaggtcgaag
     2521 tgggcgtggc cgaggaggag gaagtacggg ccatccacgt gccgcgctac cacgtggtgc
     2581 tcttcgacga cgacgaccat tcgtacgagt acgtcatcga gatgctgatg gcgctgttcg
     2641 gccacccgct gcgcacggcc tacgacatgg cgtcgaccgt ggaccgggcg gggcgcgtga
     2701 tcgtggagac gaccacgaag gaacgcgccg agctcaagcg cgaccagatt cacgcatatg
     2761 gcccggactg gcgcattccg cactgcgtcg gcccgatggc ggcggacgtg gagccggccg
     2821 agtagccgcc gcgactgcgt ccgacgagaa cgccccgacc gcggcggccc tcccggagcg
     2881 accgcgcatt ccgccgcgac ccgccctact ccacctccac cacggcctcg acctcgacgg
     2941 cgatgcccgt gggcagggac gccatcccga ccgccgcgcg ggcgtgcagg ccccgatccc
     3001 cgaaaacggc cgccatcagg tccgaatagc cgttgatgac ggccgagtgc tgcgtgaagt
     3061 ccgccgtcgc gttcaccatt cccgtcacct tcaccacgcg gcgcacgcgg cgcaggtcgc
     3121 cgagctcggc ctggagcacg gccaggtgct ggatcgccgt gagccgggcg gcctcgtagc
     3181 cctcctcgac cgagaggcca tcgccgagcc ggccggtgac ggacgtgccg tcgggtcgcg
     3241 tcggcccctt tccggccaga aagagcaggt cacccgagcg gacggcgttc acgtacgacg
     3301 cgatgggcgc gcccggcgcg ggcagcgtga tcccgagctc ggcgagccgc gcatccgggt
     3361 cgaagtccgg gtcgagcgtg gcctcggggg cgggcgccgg cgcctcgggc ggaacgggcg
     3421 cgccgcacgc ggcggcgagg agaagaatcg cggcgaggat gcgcatcgag gggcagtctg
     3481 gatattcgac cggacaagaa taggctgccg tggcggaaac cgcatccagt cggtccgtca
     3541 aaaacgcccc tttcaccccc cgggattccc cgacgccgcc gatcgcccgc gggctgtaca
     3601 ttcgcggctc gacgaaaccg ccggccaccg ccatgaagcg cttcacgacc tccgcactgt
     3661 tcctgctcgt cgcgatgtcc gcggccgcac agccgtggat ccggcgcccg accggcccgg
     3721 actacccgac cggcgacaag gtcatcgagg ccatctggga cgagggcatg aaccgctcgc
     3781 aggccgcgga cctcgcgcag gtgctggccg acgtcatcgg cccgcgcctc acggccgcgc
     3841 ccgagcacgc gaagggagtg gaatgggccg tcgggacgct cgaggcgtgg ggcattccgg
     3901 ccgaggcgga gccctacggc acgtggcgcg cctggcgccg cgggacgagc cacgtggacc
     3961 tcctcgagcc gcgcgtgcgc tcgctcgagg ccgtgatgct cggctggagc cccggcacgg
     4021 gcggcccgcg gcagggcggg accgtgctgc tgcccgaggc cgaaggcccg gaggccttcc
     4081 gcgcctggct gccggacgcc cgcggcaaat tcgtcctcct gacctacgcc gagccgagct
     4141 gccgcccggc ctcgtcctgg accgagcacg ggctcccggg cgcggccgac gccttcaacg
     4201 aggcccggac ggccgcgcgc gaggcctgga ccgcccggat cgacgcgatc ggcatggagc
     4261 gccaggagat catcgacgcg ctcgaggggg ccggcgccct ggggttcctc acgaactact
     4321 ggacgggcgg gtggggcacc gaccgcatct tcccgctgac ctactcgttc caggcgatga
     4381 accgccgggc ggcggccttc aacctgtcgt gcgaggacta cgggctggtc ttccgcctca
     4441 ccgagaacgg ccagggggcc gtgctccgcg cggatgccga tgccgaggac ctgggcgagc
     4501 agcccgtcca caacgtcatc ggacgcatcg aggggacgga gcttccgggc gaggtcgtgc
     4561 tgctttccgc gcacttcgac tcgtgggacg gggcctcggg catgaccgac aacggcacgg
     4621 gctcggtggt catgctcgag accatgcgga tcctgaagaa gctctatccg aacccgaagc
     4681 ggaccatcat gatcggcctc tggggcgcgg aagagcaggg actcgtgggt tcgcgcgcgt
     4741 tcgtcgcgga ccaccccgag atcgtggatc acctgcaggt cgtcttcaac caggacaatg
     4801 gcaccgggcg catccggtcc gtctccacgc aggggctcac gcgcgccggc gagttcttcg
     4861 cccgctggtt ttcgcagatg cccgagatcc tggtcgggga catcgacctg aacgtgccgg
     4921 gaagcccggg cggcggcggc tcggactacg cggccttcat ctgcgccggc gcgcccgcgt
     4981 tctcgctctc ctcgacgtcg ttcgactacg gcacggccac gtggcacacg aaccgcgaca
     5041 ccttcgacaa gctctccatc gaggacgtga agcggaacgc gaccgtgacg gcgatgctca
     5101 catacctggc ctccgaggac gagacgatgc cgcgcgaccg gcgcgagctc ggcaacgatc
     5161 cggagacggg cgagccgctc gagtggccca cgtgcgagga cggggcgcgc gagacgacgg
     5221 ggcgcttccg gtagcgcgcc cgtgtccgcc gggccgactt ggcgggcgcc tcgccccgaa
     5281 gcctcagtcg cggatcttgt tcacgatgac cagcatcgcg gcgccggcca cgccccccag
     5341 gacgcccagg tacccgttcg cctgcgcgcc cacgaaccac gaccactgct cgtcgaggta
     5401 gcggttgcgc gcgacgggcg gcagctcccg gctgacccgc atgcggtagg cgtcgaaccc
     5461 ggccgtgagg cccacccagt cgtggacgtt cagcgtggcc cggaaggtgg cgccgggaag
     5521 gcggacgtcc acccactcgt ccaggtcgcg ggcggcgacg atcggtcccg cccccacgtc
     5581 cagcttcagg tgccgggtca ggcggtaccg gccccgcgcc ttcacggccc agagcatgtt
     5641 gcggtactcg gtgtcgtcgt acgacgcgta gagcgatgcc ccgagcgccg tgcgtccgtc
     5701 ggcccggttc ctcatcgcgc cgacctccca gaacccggtg ttgacgttgt cctggcggag
     5761 gtcgttctcg aaggcccaga ggccgccgaa ctccacgatg ccgacgacgc cgcagtcggg
     5821 cggcgcgccg tcgcggaagc agaactgggc ccgggccggg ccagggacga ggacggcgag
     5881 aacgagggca gccaggagcc ggcgccgatg actacacacg tggatggccc gtggggcggt
     5941 cgatttgccg cacggtacga tgcgccgccc gccggggtgc accgggccgg gccgaaattt
     6001 cggggcgggt gcggcggggc ggcaggcccg gcccggcagg ctcagtacga atcgtcgagc
     6061 gcgaacacgg gacgccgctt catccagagc ccgcgcgtga agtccgggaa gagctgcggc
     6121 gcgctgcccg acgcgatgga catttccgag agcggggtca cggcgctcca ggcggccgcg
     6181 tcgtaggcgt cgagcggggt cggtccctgc gccttgaccg attcgatgaa ggcgtgcagg
     6241 acgaagaagt ccatgccgcc gtgtcccgcc ccggtcgcgc gctccgcgtg ccgcttccac
     6301 agcgggtggt cgtagcggtc caggtacggc tgcgcgtctt cccactggtg cgcggggctc
     6361 ttcccctcca ggtagatgga ccggtttacg tccatccaga tcccctccgt cccctgcacc
     6421 cggaagccca gcgagtacgg gcgcggcagg ttggtgtcgt gggagacgat gatcgactcg
     6481 ccgttctgcg ttcggatcac ggtcgtaatc acgtcgccca gccggaattc gaccctggcg
     6541 ttggggtgat cctcgcccgc ctgcttcacg atgtagtcgt gcagcccccg cgccttggac
     6601 gcggtggacg tcagggacac gaagcggttg ccgcggttga cgtcgagcat gacgccgacc
     6661 ggtccgaggc cgtgcgtcgg gtagacgtcc gcgttgcggt gcaccgaatg ccacgtccgc
     6721 caccgggcct ccgagaacgc cttctccccg aattccacac ccccgccgta ggggcgcgtt
     6781 ccgtcgttga acttgacctc gcgcaggtcg tgctggtagc cgcattccat gtggacgagc
     6841 tcgccgaaga gcccctgccg gaccatgttc aggacggcca tgacgtcgcg ccggtagcag
     6901 acgttctcga ggatcatcat ggggacgccg gtttcctcgt gcgtgtcaac caggtcccag
     6961 cactcctcga gcgtattggc ggccgacacc tcgagacccg cgtacttccc ggcccgcatt
     7021 gcggccacgg ccatgcgcgt gtgccacagc cagggcgtcg cgatcaccac ggcgtcgaca
     7081 tcgctgcgcg cgaggaggtc caggaaggcg tactccgagt cgccgtagac ggcggccttc
     7141 ggtcggcccg catccgcgag ctgcttctcg gcgctcgcca gggccgccgg gtcgatgtcg
     7201 cagagcgcgg tgaccgcgac gtcgtcgcgc cggagggcga ggtcgacgtg cgagcgcccg
     7261 cgaaggcccg tgccgatgaa gccgaggcgc acggcgtccg agggtccgcg gcggacgagg
     7321 gccggggcgg ggcccgccgc ggaacgcgcg gccgccttcg gaacgagggt gtaaccgacg
     7381 ccggccagcg tgcccgcggc gaggaatttc ctgcggttca tggagtgtcc ggatgcgttc
     7441 gcggggaata tcgcgcccga tggcttggcg cgcaccgggc aacggcggtg tccggcgacc
     7501 tgccgcgccc cgcgtagagg ccggcgccgc cccttgcccg cattgttgcc ccgatcccgt
     7561 accttccgcc cttcatccga ccggccggcg catgcagctc tcgacgctcg actgggccat
     7621 catggccgcg ttcttcgcgg tctccctgat catcggcgtg gccgtggccc ggcggtcggg
     7681 tcgcgacttc tcggccttct tcctggccgg ccgatcgatg ccctggtggt tcctgggcat
     7741 ctcgatggtc gcgaccacgt tttcgaccga cacgccgaac ctggtcacgg acatcgtccg
     7801 ctcgaacggg acctacggca actggacatg gtgggccttc ctgctcaccg gcatgctcac
     7861 cgtgttcctg tacgcccgcc tttggcggcg ttcgggcgtg ctcacggacg tcgagtttta
     7921 cgagctgcgc tactcgggcc ggatcgccgc cttcctgcgc gggttccgcg cggcgtacct
     7981 gggcatcgtg ttcaacgtcc tgatcatggc gtctgtgacg ctggcggcca tcaagatcgg
     8041 ctcggtgatg ttcggctggt cgccgctccg cacggtcacc gtcgcggcgc tcgtcaccat
     8101 gacctactcc gcgatcgggg gcctgcgtgg cgtggtcctc acggacctcc tgcagttcgc
     8161 gctggccatg atcggctcga tcggcgccgc ggtctacgtg ctgggactgc ccgaggtggg
     8221 cggactcgac gggctcctcg cgaacgggcg cgtgcgcgag gccatggact tcctgcccga
     8281 cttcgcgacc ctcggctggg agcaggtgct gcccgtgttc atcgtgccgc tggcggtcca
     8341 gtggtgggcg gcctactacc cgggctcgga gccgggcggc ggcggttacg tggtacagcg
     8401 catgctctcg gcccggaacg agcggcaggc ggccggggcc acgcttctct tcaacacgct
     8461 gcactacgcg ctccgcccct ggccgtggat cctggtggcg ctcgcctcgc tcgtggtgtt
     8521 ccccgacctg gcctcgatcg ccgcgcggtt cccgcacgtc cccgagcagg tggtccgcaa
     8581 cgacctggcc tacccggcca tgctcacctt ccttccggcc ggcctcctgg gcatcgtggt
     8641 gacgtcgctg gccgccgcct acatgtcgac cgtgtcgacg caggtgaact ggggttcgtc
     8701 gatcatcgtg aacgacctct accaccgctt cctgaaccgc gaggcgagcg agcggcagca
     8761 ggtgtgggcc ggccgggtcg ccacggtggt gctcatggtg acggcctgcg gcctggccct
     8821 gctcctggag aacgccctgc aggtcttcaa catcatcctg cagatcgggg cgggaacggg
     8881 cctcctgtac atcctccggt ggttctggtg gcgcatcaac gcccaggccg agctcgtggc
     8941 catggtcgcg tcgttcggcg tggccctgtg gttcgcgttc gtgtcggacc atggcctgga
     9001 ggggtgggcc cagctcgtga ttggggtggc cgtcacgacg gcggcctggg tcaccgtggc
     9061 gttcgtcacg aagccgaccg atccacgggt gctcgacgcg ttctaccgga agatccatcc
     9121 gggcgggccc ggctggaagc cggtcgaggc gcgcgtcggc aagccggaag gctcctcgga
     9181 cctgccgtac gcgctcgtcg ccgctctctg cggcgccgtg gccatctacg gggcgctctt
     9241 cgcgaccggc ttctacctgt acggccgggc gggcgccggg atcgggatga ccgtggtgtc
     9301 cctggcagcg gccgcggggc tcgcggccgt ctggaggcgg ctggcgttcc ggtagccact
     9361 gcggcgccgg cgcggcggag ccgaaggccc gcgggccgtc accacaccgg acgtccgtcc
     9421 agccggagcg caccgggacc cgtgggctcc gggaagaagc gggcgcggga cgcccgccgg
     9481 ggaaacgcgg cgacgtattc gttgcgccag gcctccagaa aacggtccga gtcagcccgg
     9541 gggaccatcg cccacaccgc cccgccgaag cccgccccga acgacgacga ggcgacggcg
     9601 ccgagctccc gcgccgacct cgcgaggtgc atcgtctcgg ggacctggtt gccgagcagc
     9661 tcctcggccg cgtgctggct tcggtcgacg gccgcaccga acgcccagat gtcgccacgc
     9721 gagagcgcat cccaggcggc gggaaccacc tcggcatgtt ccacaccgaa atgccggacg
     9781 cgtcgcagga gggcgtccgt cccctcgccc gcggggagag cctcggcgag ccgccgcgcg
     9841 gcatcggggt cgcgttccac gagatctccg agatggccgg cctcctcccc cgtcgatgcg
     9901 gtccacgccg cgaccgcctc gcgcgccagg tcggacgccc gattgtacct ggccctcgcg
     9961 gaccccgtct tgttcgccgt gaccccgctc gctccgacga ccagcccgag atgccgcgga
    10021 atctcgacgc ttccctccct gcggaaaggc gcgtaccggt atcgcccgat gtgacccgac
    10081 cgggagagca ggatggccac gtggtcctcc gatccgcccg aggtgcccac gccgcgttcc
    10141 cccgcgagcg ggccgaaccc gctcccgttc tcgaccgagg cgaggtagcc ggccagggcg
    10201 tccgcgtccg gaagcgcggc cgccagcggt ccgccgcgtc ggaggccgcc cgccgcgtcg
    10261 aggcccagga acacggcgac gatgaacgca ctggagctgc tcatgccggc tgcccgcggc
    10321 aggtcgctcg agagccacag gtccgcgccc tgcaggctgc cgggcgcatt catcgccacg
    10381 cggcggatca ccgcggcggc gtagttcgac cagtggccgg gggcggcgga ggcgccgacc
    10441 cccgcctcga gcgccgcgta gcgcccgaga tccggctcat ggatgcgcac caggccgtcg
    10501 tcgcgcggca cggccaggac ccggatgcat cgggcggacg cacaggtcag gctcgatccc
    10561 cccgcatagt cggtgtgctt gcccaggatc tcgatccgcc cggggaccag gagccggtgc
    10621 cgctcgcgcg cgtcgtcgcc ggcggccgcg tcgagctcgc cgagcgcctc gaggtgacgc
    10681 gcggcggtgg ccgggtccat gcccgaggcc gccaggatat cgtccggtgt ggtcacgggg
    10741 gcattcgtcg gcgggcagga tacggcggga cggggcgacc ggccagacgg cacggcagcg
    10801 ggctcccgcg cccggcgcgg agggtcggtg tcgccagggc ccggggccgc ggcaggcgca
    10861 ctacgacgga tcccgctcca ggttgtggcg gggtctccgg ggcgctcacg gcccggcggc
    10921 tgcgggcccc ggacccccac gaggaacgcc gcgaggcggg ggcaaaaacg ggctttaacg
    10981 gcctccaatc gccgattcgg ggcccgtctc tttatgcgat cttttcttgc cgcctatgga
    11041 gggggggact aacttgcgcc ccgattcagc gcaccggtga cgctcttcgg accggaacgg
    11101 cgacccgatc gcgcgcgcac ctccagccca tccccatcat gatcgggccg atcctgttct
    11161 tcgtcttcgc catcacggcg atcgccggcg cgctgggcat gttgctgtcg cgcaacccgg
    11221 tgtcgagcgc cctctggctg atcctcaccc tcttctgcat cgcggggctg tacctgacgc
    11281 tcaacgcgac cttcatttcg gtggtccagg tgctcgtgta tgccggggcc atcatggtgc
    11341 tgttcgtgtt cgtgatcatg ctgctgaacc tcgccgacct gccgaggctg aagaccgtcg
    11401 gctggcgacg cgtcatcgcg ttcgcgctgg ggctgggcgt gctcgcgcag ctcgcctacg
    11461 tgaccgccgc ggcgctcgac gtcctgccgg cgccggtctc gctcgaggcc gcggccgaga
    11521 gcggctcggc cgccgtgctc gcccgcgtgc tcttcacgca gtacgccttc ccgctcgagg
    11581 tcatcggaat cctgctgctc gtcgccacga tcggcgccgt catgctcgcc aagcgccgct
    11641 tcgtgtagcc ggcccactcc cagcccgtct ccccatggaa gtcaccatca actggtacct
    11701 gaccctgagc gcggtgctct tcgtgatcgg ggtcctgggc gtgctgctgc ggcgcaacgc
    11761 catcgtgatc ctgatgtccg tggagctcat gctcaacgcg gtcaatctca cgctcgtgac
    11821 cttcgcccag tcgatgggcg acaccgccgg ccagatcctc gtgtttttcg tgatgtcggt
    11881 ggcggcggcc gaggcggccg tcggcctggc catcgtgatt gccatcttcc gcaatcgcct
    11941 gaccgtggac gtctcgcaga tcaacctctt ccggtactga cggagcgccg cgcgcggtca
    12001 ttccgaacct ccctctcatg gatatcgacc ctctcgtcct gctgatcctg ctcctgccgc
    12061 tggccggagc ggtcctgaac ggtctcgtcc cgctgttcgt gccgtcgctt cgcggccggg
    12121 agacgctcat cggcaccctc ggcacgctcg tagtggcggt gccgttcgcc ctcacggtgg
    12181 ccctgttcct ggggttcgag ggcacgaccc acatcacgca ggtcgcgccg tggatctcgg
    12241 cgggcgactt ctccgtctcc ttcgcctacc ggatcgacgc gctgtcgctg accatggcga
    12301 tgatcgtgtc gggcgtcggc ggcatcatcc acctgtactc gatcgggtac atgcacgggg
    12361 acccggggtt cttccggttc ttctcgtacc tgaacctgtt catcttcatg atgctgaacc
    12421 tggtcctcgg agacaacctg ctggtgttgt tcctgggctg ggagggcgtc gggctctgct
    12481 cgtacctgct catcgggttc tggtacacgg acctgaagaa ctcggcggcc ggcaacaagg
    12541 ccttcatcat gaaccgggtg ggggacttcg ccttcctttt ggcgatgttc atcttcgtgc
    12601 gcgagctcgg cacgctggac ttcggcacgc tgctcgccga gggcccgtcg atggatccgg
    12661 cgacggtgaa ctgggccgtg ctgctgctct tcatcggagc gaccggcaag agcgcacaga
    12721 ttccgctctt cacgtggctc cccgacgcca tggccggccc gacgccggtg tccgcgctga
    12781 tccacgcggc gaccatggtc acgagcggcc tgtacatgct ggcccgcatg tcgccggtgg
    12841 tgctcgccgc cccgaccatc atggcggtga tcgcgatcgt gggcgcgctc acggcgatcg
    12901 tcgcggcgac catcgccatc acgcagaacg acatcaagaa ggtcttggcc tactccacgg
    12961 tgtcgcagct gggctacatg tgcctcgccg tggggatggg cgcgtgggtc tcggcgatgt
    13021 tccacgtgat gtcccacgcc ttcttcaagg cctgcttgtt cttgggatcg ggcgcggtca
    13081 tcgaggcgtg tcaccacaac cagg
//
LOCUS       DBNHQZ010000332         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_912326, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000332 DBNHQZ010000000
VERSION     DBNHQZ010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_912326"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC16YU_08895"
     CDS             complement(<1..563)
                     /locus_tag="AC16YU_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6065382.1"
                     /translation="MTPEAGSNGTGVERKVQTIKQHVVEIVSDSGEGAQKAGQAFGTI
                     SAKMGNGIWTVEIIPAEIQPPPRNPAGASGIRVRLGSRPVTNMGDEVDLIVAFNEQAL
                     LGRLDLTRFRPGATILLEDKWRGHADPEIAAAYGSTYARLVEEGYSVIEVPMEEICLR
                     YVNNPQLGKNMFVLGMLCAVYNRNMDFA"
     gene            915..1385
                     /locus_tag="AC16YU_08900"
     CDS             915..1385
                     /locus_tag="AC16YU_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065383.1"
                     /translation="MRTRRPAFLLLAAALLAVPACDSNDEKTDIEGLWLQSGIDVIYY
                     HVERGTITIYDYMGDEYDEGPDCYIISELDIVRRDGTKVTVSSPDLPGIQIVFDLERD
                     GETLLMTLSGETATLEKSSLNVSTFEEKECTGADLLSGKDAAGERAPKPGLPIG"
     gene            1397..>2532
                     /locus_tag="AC16YU_08905"
     CDS             1397..>2532
                     /locus_tag="AC16YU_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR6065384.1"
                     /translation="MRPETGNPREEKARQGGLSPLAYAELPPGTEYPPYVPPSSSPAE
                     FTLKALLAGIVFGVLFGSANAYLGLRVGLTISTSIPVAVMTIALFRSLGVFGVRSTIL
                     ETNFSQTVGSASSSLASGVIFTLPALFMWGFSPGLLQMTFLALCGGLLGVLFMIPLRR
                     SLIVGEHGRLPYPEGTACAEVLVASESGGGRARNIFLGLGTGMLFKLVFGLLKVLPGD
                     FTVRLPGLKKGEVASEASSALVGVGFILGPRIAAVMVGGGLLSWVVIIPVIAEIGDGL
                     ASPVFPETVRLIRDMSAHDIWTRYVRYIGAGAVAMAGLMTLVRSLPTMVRSFRIGARQ
                     IAERIGERSATGSGAGGPWAGILRTEQDLPLKVVGLGALAVAAVL"
BASE COUNT          373 a          815 c          891 g          453 t
ORIGIN      
        1 gcgaagtcca tgttccggtt gtagaccgcg cagagcatcc cgagaacgaa catgttcttc
       61 ccgagctgcg ggttgttcac gtaccggagg cagatctcct ccatcgggac ctcgatcacg
      121 ctgtaccctt cctcgaccag gcgggcgtag gtgctcccgt acgccgcggc gatctccggg
      181 tcggcatgac cgcgccactt gtcctcgagg aggatcgtgg ctcccggcct gaaccgggtc
      241 aggtccaggc gaccgagcag ggcctgctcg ttgaaggcca cgatgaggtc cacctcgtcg
      301 cccatgttgg tgacgggcct ggatccgagg cggacgcgga tgccgctcgc gccggccgga
      361 ttgcgcggcg gcggctggat ctcggcggga atgatctcca cggtccagat cccgttgccc
      421 atcttggccg agatcgtgcc gaaggcctgg ccggccttct gggctccctc accggagtcc
      481 gacacgattt cgaccacgtg ctgcttgatg gtctggacct ttcgttcgac gccggttccg
      541 ttcgagccgg cttctggtgt catggcagag gacatgcgcg cggggtgcgg ttccgtggtg
      601 tttcgtggtc tgggcggccg ggggaaagcc gtccgaaaat cccacgcaac agtaccgaat
      661 tgtggctcgt gagcgtgtag gctccccggt acagggggtg tagggggccg tcgcccacct
      721 ccgtggggga ttccccggcc ccggttgtgg ggtcgagagc gtgagggccg ggaggcctgc
      781 agcgaagcgt ccggggcggt gcttccgctg ccgggcgata tcgctgctgc cctcgcagat
      841 catcgaaata tgtgctattg tcgggacagt cgcgcgacct ggcgccccaa tccataaaca
      901 ttccgagagc cttcatgcgc acccgccgtc cggcctttct gctgctcgcc gcggcccttc
      961 tggccgtccc tgcctgcgac tcaaacgatg agaagacgga catcgagggc ctgtggctcc
     1021 agtcgggcat cgatgtcatc tactaccacg tcgagcgcgg cacgatcacc atctacgatt
     1081 acatgggaga cgaatacgac gagggccccg actgctacat catctcggag ctggacatcg
     1141 tccggcgtga cgggacgaag gtcacggtat cctcgccgga cctcccgggt atccagatcg
     1201 tcttcgacct ggagcgcgac ggggagacgc tcttgatgac cctctcgggc gagaccgcga
     1261 cgttggagaa gagcagcctc aacgtcagca ccttcgaaga gaaggagtgc acgggagcgg
     1321 acctgctgag cgggaaggat gcggccggag agcgcgcgcc caagcccgga cttccgatcg
     1381 gatagccgcg ggcgaaatgc gaccggagac cgggaatccg cgagaggaga aggcacgcca
     1441 gggcggcctg agcccgctcg cctacgcgga gctgccgccg gggacggagt acccgccgta
     1501 cgtcccgccc tcgtcctccc cggccgagtt cacgctgaag gcccttttgg ccggcatcgt
     1561 gttcggcgtg ctcttcggat cggcgaacgc gtacctgggg ctcagggtcg ggctcacgat
     1621 ctcgacgtcg atcccggtcg cggtgatgac catcgcgctc ttccggtcgc tcggcgtctt
     1681 cggggtccgg tcgaccatcc tcgaaaccaa tttctcgcag acggtcggct cggcgtcgag
     1741 ctcgctggcc agcggagtca tcttcacgct gccggcgctc ttcatgtggg gcttcagccc
     1801 gggcctgctc cagatgacgt tcctggcgct gtgcggggga ctcctgggcg tgctcttcat
     1861 gatcccgctc cggcgctccc tgatcgtggg cgagcacgga aggctgccct atcccgaggg
     1921 aacggcgtgc gccgaggtgc tggtcgcaag cgaatcgggc ggtggaaggg cgcgcaacat
     1981 cttcctgggc ctcggcacgg gcatgctctt caagctcgtg ttcggcctgc tgaaggtgct
     2041 gcccggcgac ttcaccgtac gactcccggg cctgaaaaag ggcgaggtcg cgtccgaggc
     2101 gtcgtctgcg ctcgtgggcg tcggcttcat cctgggcccc cgcatcgcgg cggtgatggt
     2161 gggcgggggc ctgctctcgt gggtggtgat catccccgtg atcgccgaga tcggggatgg
     2221 cctcgcgtcg cccgtgtttc cggagaccgt gcgcctgatc cgggacatga gcgcgcacga
     2281 catctggacg cgctacgtcc gatacatcgg ggcgggcgcc gtggcgatgg cgggcctgat
     2341 gaccctggtc aggagcctgc cgaccatggt gcggagcttc cggatcgggg cgcgccagat
     2401 cgcggagcgg atcggcgagc ggtcggcgac gggcagcggg gcgggcggac cgtgggcggg
     2461 gatcctgcgc accgagcagg accttcccct gaaggttgtc ggtctcgggg ccctggccgt
     2521 ggcagccgtc ct
//
LOCUS       DBNHQZ010000333         7059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2942541, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000333 DBNHQZ010000000
VERSION     DBNHQZ010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2942541"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16YU_08910"
     CDS             complement(<1..307)
                     /locus_tag="AC16YU_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065385.1"
                     /translation="MRNRLDLLAVATLVLSARPARGQYYDSQVLQKSFETTEFFFRPV
                     WMNPFGLGSFGRSAAGFLRDPLVDLQLGPSRIAADSSGRDLVYIDFRSTSETRSQSGG
                     "
     gene            complement(337..1104)
                     /locus_tag="AC16YU_08915"
     CDS             complement(337..1104)
                     /locus_tag="AC16YU_08915"
                     /inference="COORDINATES: protein motif:HMM:NF040341.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HIR6065386.1"
                     /translation="MRTLTLTLALLASGCGLFGSSGQDPDLAAARLRWNALGWDDYDM
                     TLMRGCFCPGAGTHEVWVRDESVVAVWPAGEYPVEFAPVWWEHMPTVEELFDLIDRAD
                     QEADHLEVEYDDAGWPSRIDIDWWAEAVDDEISYRVESVAEAAASHTVTLAPGDTFTG
                     GGTTIRFDAVTADSRCPLQVECIWAGEATASFTATLPDGSARTFDLTDPPGSFGEDRS
                     LEVGSLRVTLITVTPYPEEPGTIDADAYRVRLLIESL"
     gene            complement(1113..2333)
                     /locus_tag="AC16YU_08920"
     CDS             complement(1113..2333)
                     /locus_tag="AC16YU_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR6065387.1"
                     /translation="MKTLRLALFAAALLVAACDTAPTADGPEPFQARDLTAAEKAVVA
                     ADNRFGFALLEKLADAAPGDNVFISPISVSMALGMTVNGAAGDTYDAMVRTLGKDHLD
                     PDEVNRSYRYLKDLLPGVDPEVTVEIANSIWYRMGFPVLASFLEAGETWFDAESREDD
                     FSDPAAVDRVNGWAEEKTHGMVQDVITEIDPAVVMLLMNAVYFKGTWQYQFDPDDTTE
                     GPFTNADGTVSQVPMMRLEATLPTLFTDRFSAVDLPYGDSLWSMSILVPSEGTTAHEL
                     VADLDSETWNAWASQFQLRTLTLSMPRFEADYKESLVPALTALGMGVAFDPQRADFSR
                     IATGGGLYIGDVIHQTAVIVNEEGTEAAAVTVVVIDVTSVGPRISVDRPFVFLIRERS
                     SGTILFIGIINTLA"
     gene            complement(2330..3298)
                     /locus_tag="AC16YU_08925"
     CDS             complement(2330..3298)
                     /locus_tag="AC16YU_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6065388.1"
                     /translation="MERRMHPESHESTGGIDPVLARLAEASRAGSFDPGFEGRVMARI
                     RAERSTAAGAPPGRPARIRPLRWAAVAAVFLALATTALVLDGARWRTLTAAPGETLTR
                     TLPDGSLVTLHAGSTLRHRPFRFRGERLVRLEGEAFLDVVGSGRRFVVETFNAEVIVT
                     GTRFNVRAWPGDEASETAVALVEGSVVVRSRRMVEPSMTFQRSDPTEVAIVAGETARV
                     ASGAAMRADDAPAPGDAANWTGGGLSFRDRPLADVLRALERRYAVRIHADPSVTDGRT
                     ATYLNPESPALPDVLDALSFALGLRYGRTADGWILTPASPEADGTP"
     gene            complement(3276..3905)
                     /locus_tag="AC16YU_08930"
     CDS             complement(3276..3905)
                     /locus_tag="AC16YU_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6065389.1"
                     /translation="MNANRNRGMHPSDRASADRSAEPSEAELVDRARAGDDAAFRALV
                     VRYEPLVAATATGMLGRGPEAEDVGQETFVRFWRSLDRFRGDSTVGTYLTRIAMNLSL
                     NVLKRRTRMRRWFPGGDLPDRPDASAGASREVERSETARLVDDAIRTLAPPHRAVVVL
                     RLVQGLSTAETAEALGIPTGTVLSRLSRAQRQLRDKLAPIVAHGTPDAS"
     gene            complement(4019..5338)
                     /gene="mtaB"
                     /locus_tag="AC16YU_08935"
     CDS             complement(4019..5338)
                     /gene="mtaB"
                     /locus_tag="AC16YU_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR6065390.1"
                     /translation="MPRVALHTLGCKLNYAETGAIGRGFRERGFDVVEAGTPADVAVI
                     NTCTVTDEAERKCRQAIRRVLRARPDTFVVVTGCYAQLRPDELAAIPGVDLVLGSQEK
                     GHLFRYVDSFRKVERTQVHVSCIDQVRDFSPAHAAGERTRAFLKVQDGCDYSCSFCTI
                     PRARGRSRSQSVAGAVAQARSIAGEGFREIVLSGVNIGLFGEDTGESLLDLLRGLDAV
                     EGVTRYRISSIEPNLLTDDIIDFVAGSRAFQPHFHVPLQSGDDEVLGRMRRRYRRGAY
                     ADRVERIARAMPDASIGADVIVGFPAETADRFLGTVSFLESLPVAYLHAFTYSERPDT
                     PAVRELGRTLAPVPKEERSRRNRALRMLSERKHAEFVRRHLGTSRSVLWEEPGQGRTV
                     EGYTDNYIRVRADADGRRAGSVEAVTLVDLSEDGAALAGGPTLPVLG"
     gene            5430..6128
                     /locus_tag="AC16YU_08940"
     CDS             5430..6128
                     /locus_tag="AC16YU_08940"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIR6065391.1"
                     /translation="MFAREGYVLIASAVAVSVVLAVLGWQAGTLLRSALWVLAVLVTV
                     FTLWFFRDPRRTPPAGAGALLLAPADGRVVEIVEEEEPLFLEGPARRISIFLSPLDVH
                     VNRIPADGVVAFDRYVPGEYLVAWNPKASELNERSQLGLVHPSGVRVLFKQIAGAVAR
                     RVVYHVQVGDTVRAGDRFGIVKFGSRMDVLVPPHVPVFAEVGDRVTGGVTILGHLFAA
                     LDTEGGPDAGIQAP"
     gene            6106..6957
                     /gene="pssA"
                     /locus_tag="AC16YU_08945"
     CDS             6106..6957
                     /gene="pssA"
                     /locus_tag="AC16YU_08945"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR6065392.1"
                     /translation="MPGFRRRNPDPGQKQRRIRRRLEAYRAARAGRVRRRLSPVAVPS
                     FFTLMNLFSGFLSLIQTLEGNLQGAVWLIVLAGFFDVLDGMMARLTNGSSLFGVELDS
                     LADIVSFGVAPAFAVYVFGLRDAGGMGLIVSSLPAVCGAVRLARFNIQFDGEKKDYFR
                     GLPIPAQAAALMVLMLTMHDNEWFARFGPSGMSILVPAVIVLSALMISTIRFDTIPRP
                     SARYLRQHPRKAMLFGISLVLLLVLHQIGLLVVMAGYLAIGIGRAAVNLVRAVYAADP
                     DAASPDS"
BASE COUNT          983 a         2433 c         2591 g         1052 t
ORIGIN      
        1 tcccgccgct ctggctgcgg gtttcgctgg tcgagcggaa gtcgatgtac acgaggtccc
       61 ggccgctcga gtcggccgcg atccgcgacg gcccgagctg caggtccacg agcgggtcgc
      121 gcaggaagcc cgccgccgag cggccgaagc tcccgagccc gaacgggttc atccacacgg
      181 gccggaagaa gaactccgtc gtctcgaagg acttctggag gacctggctg tcgtagtact
      241 gtccccgggc cggccgggct gagagcacga gcgtggccac ggcgagaagg tccagtcggt
      301 tgcgcatgga aggggcgcct cgcggcgcgc gttggatcag agggattcga tgagaaggcg
      361 cacccggtag gcgtcggcgt cgatcgtgcc gggctcttcg gggtagggcg tgacggtgat
      421 gagggtgacg cgcagcgatc cgacctcgag cgaccggtcc tcgccgaacg atcccggcgg
      481 atccgtgagg tcgaacgtcc tcgcggagcc gtccggaagc gtcgcggtga acgaggccgt
      541 cgcctcaccc gcccagatgc actcgacctg gagggggcag cggctgtcgg ccgtgaccgc
      601 gtcgaagcgg atcgtggtgc cgccgccggt gaacgtgtcg cccggcgcga gcgtgaccgt
      661 gtgcgaggcc gcggcctccg cgaccgactc cacccggtac gagatctcgt catcgacggc
      721 ctccgcccac cagtcgatgt cgatgcggga cggccagccg gcgtcgtcgt actccacctc
      781 gaggtggtcc gcctcctggt cggcccggtc gatgaggtcg aagagctcct ccacggtggg
      841 catgtgctcc caccagacgg gcgcgaattc gaccgggtac tcgccggcgg gccagacggc
      901 cacgaccgac tcgtcgcgca cccacacctc gtgcgtgccg gcgcccgggc agaagcagcc
      961 ccgcatcagg gtcatgtcgt agtcatccca gccgagagcg ttccagcgca ggcgcgcggc
     1021 ggcgagatcc gggtcctgtc cagacgagcc gaagaggccg catccggacg cgagcagggc
     1081 gagggtgagg gtgagcgtgc gcatggcttg gatcaggcga gggtgttgat gatgccgatg
     1141 aagaggatgg tgccggacga ccgctcgcgg atgaggaaca cgaagggccg gtcgacgctg
     1201 atccggggcc cgacggacgt cacgtcgatg acgaccaccg tgacggccgc cgcctcggtg
     1261 ccctcctcgt tcacgatgac ggcggtctgg tggatgacgt cgccgatgta gagcccgccg
     1321 ccggtggcga tgcgcgagaa gtcggcccgc tgcggatcga aggccacgcc catgccgagc
     1381 gcggtcagcg cggggacgag cgattccttg tagtcggcct cgaagcgcgg catggacagg
     1441 gtcagggtcc gaagctggaa ctggctcgcc cacgcgttcc acgtctccga gtccagatcc
     1501 gcgacgagct cgtgcgcggt cgtgccctcc gacgggacga ggatcgacat ggaccagagc
     1561 gagtcgccgt agggcaggtc cacggcggag aagcggtcgg tgaaaagggt gggcagcgtg
     1621 gcctcgagcc gcatcatcgg tacctgggac accgtcccgt cggcgttcgt gaatgggccc
     1681 tccgtggtgt cgtccggatc gaattggtac tgccaggtgc ccttgaagta gaccgcgttc
     1741 atgagcagca tgaccaccgc ggggtcgatc tccgtgatga cgtcctgcac catgccgtgc
     1801 gtcttctcct cggcccatcc gttcacgcgg tccacggcgg ccgggtccga gaagtcgtcc
     1861 tcgcgcgact ccgcgtcgaa ccaggtctcg cccgcctcca ggaaggacgc gaggaccggg
     1921 aagcccatcc ggtaccagat cgagttggcg atctcgacgg tgacctcggg gtcgacgccc
     1981 ggaaggaggt ccttcaggta ccggtaggac cggttcacct cgtccggatc caggtggtcc
     2041 ttgccgagcg tccggaccat ggcgtcgtac gtgtcgcccg cggccccgtt gacggtcatg
     2101 ccgagcgcca tcgagaccga gatcggcgaa atgaagacgt tgtcccccgg ggcggcgtcg
     2161 gcgagcttct cgaggagcgc gaaaccgaag cggttgtccg cggcgacgac ggccttctcg
     2221 gcggccgtga gatcgcgcgc ctggaagggt tcgggcccgt cggcggtcgg cgcggtgtcg
     2281 caggcggcga ccagtagcgc ggcggcgaag agggcaaggc ggagcgtctt catggcgtgc
     2341 catcggcctc gggcgaggcc ggagtcagga tccacccgtc ggccgtccgg ccgtatcgca
     2401 ggccgagtgc gaaggacagg gcgtccagca cgtcgggcag ggcgggggat tcggggttca
     2461 ggtaggtggc ggtgcgcccg tcggtcaccg acggatcggc gtggatccgc acggcgtagc
     2521 ggcgctcgag ggcccgcaga acgtcggcga gcgggcggtc gcggaacgag aggccgccgc
     2581 ccgtccagtt ggcggcatcg cccggtgcgg gggcgtcatc ggcccgcatc gcggcgcccg
     2641 acgccacgcg cgcggtctcg ccggccacga tcgcgacctc ggtggggtcg gaccgctgga
     2701 acgtcatcga gggctcgacc atccggcggg aacggacgac gaccgaccct tcgacgaggg
     2761 ccacggccgt ctcggatgcc tcgtcgccgg gccaggcccg gacgttgaag cgcgtgccgg
     2821 taacgatcac ctcggcgttg aaggtctcga ccacgaagcg ccggccggac cccaccacgt
     2881 ccaggaaggc ctcgccctcc aggcgaacga ggcgctcgcc gcggaaccgg aacggccggt
     2941 ggcgcagggt cgatccggcg tggagcgtga ccaggctgcc gtcgggcagc gtgcgcgtga
     3001 gcgtctcgcc aggcgccgcg gtgagcgtgc gccagcgggc gccgtccagg acgagcgcgg
     3061 tggtcgcgag cgccaggaag acggcggcga cggcggccca ccgcagcggg cggatccgcg
     3121 ccgggcggcc cgggggcgca cccgccgcgg tggaccgctc cgcgcggatc cgggccatca
     3181 cgcggccttc gaatcccggg tcgaacgatc ccgcccggct cgcctccgcg aggcgggcca
     3241 ggaccgggtc gatcccgccg gtcgattcgt gggattcagg atgcatccgg cgttccatgg
     3301 gcaacgatgg gtgcgagttt gtcgcgcagc tgtcgctgcg cacgggagag acgggaaagc
     3361 acggttcccg tgggaatgcc cagggcctcg gcggtctcgg cggtggagag cccctggacg
     3421 agccgcagca cgaccaccgc ccggtggggc ggcgcgagcg tccggatggc gtcgtccacc
     3481 aggcgcgcgg tctcgctgcg ctcgacctcg cgcgaggcgc ccgcggacgc atccgggcgg
     3541 tccgggaggt cgcccccggg gaaccagcgg cgcatccggg tccgtcgttt cagcacgttc
     3601 agcgagagat tcatcgcgat gcgtgtcagg taggtgccga ccgtcgagtc gccccggaac
     3661 cggtcaagcg agcgccagaa ccggacgaac gtctcctggc cgacgtcctc cgcctccgga
     3721 ccccgcccga gcatgcccgt cgcggtggcc gccacgagcg gctcgtagcg gaccacgagc
     3781 gcccggaagg ccgcgtcatc ccccgcccgg gcccggtcga ccagctccgc ttccgacggc
     3841 tcggccgatc gatccgctga ggcccggtcc gatgggtgca tgccccggtt tctgttggcg
     3901 ttcacgggtt ggacaaccga tagcggctcc gtattcctcc gaccggcaag aaacgaaagc
     3961 ggcgcgcggc ggactgacgg gggacgccgg tccgtcggcg tggggcgccg gccggtggtc
     4021 agcccaggac ggggagcgtc gggccgcccg ccagggccgc tccgtcctcc gagaggtcga
     4081 cgagggtcac ggcctcgacc gagcccgccc ggcggccgtc ggcgtccgcg cggacccgga
     4141 tgtagttgtc ggtgtagccc tcgaccgtgc ggccctgccc cggctcctcc cagagcacgc
     4201 tccggctcgt ccccaggtgc cggcggacga attcggcgtg cttgcgctcc gagagcatgc
     4261 gcagcgcccg gttgcggcgc gaccgctcct ccttcggaac gggggcgagg gtccggccga
     4321 gctctcggac ggccggcgtg tccgggcgct cggagtacgt gaacgcgtgc aggtaggcca
     4381 cgggcaggct ctcgaggaac gagaccgtgc ccaggaaccg gtccgcggtc tcggcgggaa
     4441 accccacgat gacgtccgcg ccgatcgagg cgtcgggcat ggcgcgggcg atgcgctcca
     4501 cccgatccgc gtacgcgccg cggcggtacc gccgccgcat ccggccgagc acttcgtcgt
     4561 ccccggactg aagcggcacg tggaaatgcg gctggaacgc gcgcgatccg gccacgaaat
     4621 cgatgatgtc gtccgtgagg aggttcggct cgatcgacga gatccggtac ctcgtcaccc
     4681 cctcgacagc gtccaggccc ctgaggagat ccaggagcga ctccccggtg tcttccccga
     4741 acagccctat gttcacgccg ctcagcacga tctcgcggaa cccctctccg gcgatcgagc
     4801 gggcctgggc cacggcgccg gccaccgatt gcgaccggct gcggccgcgg gcccgcggaa
     4861 tcgtgcagaa ggagcacgag tagtcgcatc cgtcctgcac cttgaggaac gcccgggtgc
     4921 gctcgcccgc cgcgtgcgcg gggctgaagt cccgcacctg gtcgatgcac gacacgtgca
     4981 cctgggtccg ttcgaccttg cggaaggagt ccacgtagcg gaacaggtgg cccttctctt
     5041 gcgatcccag cacgaggtcc acgcccggga tggccgcgag ctcgtcgggc cggagctgcg
     5101 cgtagcaccc ggtcacgacg acgaaggtgt ccggcctcgc ccggagcacc cggcggatcg
     5161 cctggcggca cttgcgctcg gcctcgtcgg tcaccgtgca cgtgttgatc acggcgacgt
     5221 ccgccggcgt gccggcctcg acgacgtcga agccgcgctc gcggaagccg cggccgatcg
     5281 cgccggtctc ggcgtagttg agcttgcagc ccagcgtatg gagggcgacc cggggcatgg
     5341 agggaatccg gtgcgcgcct gcggcgtgac aggtatcttg aagcttgttc ttcggacgcg
     5401 gagacgtcgg aatcggagga agatagccca tgttcgctcg ggagggatac gtgctgatcg
     5461 cgtccgcggt ggccgtctcg gtcgtgctcg cggtgctggg gtggcaggcg ggcacgctgc
     5521 tgcggtccgc cctgtgggtc ctggcggtgc tggtcaccgt gttcaccctc tggttcttcc
     5581 gtgacccgcg gcgcacgccg ccggccgggg ccggggcgct gctcctcgcg cccgcggacg
     5641 gccgcgtggt cgagatcgtc gaggaggagg agcccctctt tctcgagggc ccggcccggc
     5701 gcatctcgat cttcctctcg ccgctggacg tgcacgtgaa ccgcattccg gcggacggcg
     5761 tggtcgcgtt cgaccggtac gtgccgggcg agtacctggt ggcctggaat cccaaggcga
     5821 gcgagctgaa cgagcgctcg cagctgggcc tcgtgcatcc gtccggcgtg cgggtgctct
     5881 tcaagcagat cgcgggcgcc gtcgcccggc gcgtggtgta ccacgtgcag gtgggggaca
     5941 cggtccgggc cggcgaccgg ttcggtatcg tgaagttcgg atcgcgcatg gacgtcctgg
     6001 tgccacccca cgtgccggtt ttcgccgagg tgggcgaccg ggtgacgggc ggcgtgacga
     6061 tcctggggca cctgttcgcg gcgctcgaca ccgagggagg gcccgatgcc gggattcagg
     6121 cgccgtaacc ccgatcccgg gcagaagcag cgccgcatcc ggcgccgcct cgaggcctac
     6181 cgcgcggccc gggcggggcg cgtgcgccgg aggctctcgc cggtcgccgt gccgtcgttc
     6241 ttcacgctga tgaacctctt cagcggcttc ctgtcgctga tccagacgct ggagggcaac
     6301 ctgcagggcg ccgtctggct tatcgtgctg gcgggcttct tcgacgtgct cgacggcatg
     6361 atggcccgcc tcacgaacgg ctccagcctg ttcggcgtgg agctggactc gctggccgac
     6421 atcgtctcct tcggcgtcgc gccggccttc gccgtgtacg tcttcggcct gcgggacgcc
     6481 ggcgggatgg ggctcatcgt gtcgtcgctg cccgcggtct gcggggcggt gcgcctggcg
     6541 cggttcaaca tccagttcga cggcgagaag aaggactact tccggggctt gcccattccg
     6601 gcgcaggccg cggccctcat ggtcctcatg ctcacgatgc acgacaacga gtggttcgcc
     6661 cggttcggcc cgtccgggat gtcgatcctc gtgcccgccg tcatcgtgct gtcggccctc
     6721 atgatctcga cgatccgctt cgacaccatt ccgcggccga gcgcccgcta ccttcgccag
     6781 cacccgcgga aggcgatgct cttcggcatc tcgctcgtcc tgctgctcgt tctgcaccag
     6841 atcggcctcc tcgtcgtcat ggccggctac ctggccatcg gcatcgggcg cgcggccgtg
     6901 aacctggtgc gggcggtcta cgcggccgat ccggacgccg cctcgcccga ttcctgaccc
     6961 tgcgccggcc cggtgccggc acggtcccca acccaagccc gatcccatgg agttcgtcgt
     7021 tcagatccgc gtcacccttc gtccgtccat cctggatcc
//
LOCUS       DBNHQZ010000334         8039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3621749, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000334 DBNHQZ010000000
VERSION     DBNHQZ010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3621749"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="AC16YU_08950"
     CDS             complement(<1..345)
                     /locus_tag="AC16YU_08950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6065393.1"
                     /translation="MRFTCPFILASSSPRRRTLLSRLGIPFEVDPSGVDERHDGSREP
                     SDLVEVLSLEKAVDVARRHPEGLVLGADTVVVLDGRILGKPDDAAHAADMLRALNGRT
                     HQVYSGIALVHGP"
     gene            494..1282
                     /locus_tag="AC16YU_08955"
     CDS             494..1282
                     /locus_tag="AC16YU_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6065394.1"
                     /translation="MIPITVCNHKGGTGKTTTSLMLAAALGLSGRRVLAVDLDPQGFL
                     SRLTGTPEPPAERSSHAMFEEGAPFDPGSVVSLPSFDLLPSSSRLSAALRRLNRPVDV
                     LWVREFASRYLAEYDFVLYDTAAAVTVFSLNALVASRLVVIPVLPEYQPVVGAEQTWQ
                     TALTVRKKLNPGLESPVFLLTMVDARKREHHLYRQYLRERYGASVMEPIVRTSASLAG
                     TRQDGRTAFDFDPRGRGAVDYANVADSLLARTASLELVALAAEA"
     gene            1306..3750
                     /locus_tag="AC16YU_08960"
     CDS             1306..3750
                     /locus_tag="AC16YU_08960"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6065395.1"
                     /translation="MAGLAIGVLAILATPYVLGLSAGGMFRIGPMLDPVDGLYRTARL
                     ADADPPSVATVAGLQGPVEVAWDGRGVPHIFAENDLDAVRATGYVTARDRLFQMDFLR
                     RFTSGRLAEVFGPSAVSTDRSMRRTGIEAAVRMNVEAARGDGRPTYELFAAFADGVNA
                     YLAAVEERDLPFEFRLFGYRPEPFTALDPGLIGWNLLYDLTFRGTPRVDDAAVDSLLG
                     PGAGEILFPRDNPLSRPIVPAEEAHWRNGVDRSGARGFAVRLPWTEDERETEPFADLT
                     AGLVEGKGSNNWAVGPRRSATGAPILAGDMHLALWLPSIWYELHVVTPTMDTYGVTVP
                     GVPLPIEAFNRSVGWAFTNTGADQVDHLLLVEDSTGRRYRYEDGWRDFRFEVDTLRVR
                     GRDSVVDTVRWTHWGPVVHRRGRGIAIRWVAHDTLRTFDALLAMNRARGYDEFERATR
                     LWDAPMQNILVADTAGTIAIRSTGHLPIRRDGGGAGYRDGSSPGSDWTGRVPFDDLPH
                     AVDPSRGYLTSTNQQPTDGSYPWYMGRDWRSTYRSIRIDTLLSGRDRHDPGQIASFQA
                     DVHAVQRDWFVPLLGAAGTVSARAERLRAMLEAWSGETGVERPEPLVMDVFLGVLDDL
                     VWDEPGFARIRRPADGVLYRLLTGALEGPWLDRVGTERVETSGDILAEALELAADSIE
                     AAYGPDPDGWAWGRHHRLILRHVSQTPVLQALWRGPVPFPGFESTLSPGAGRTVTHGA
                     SWRVVVDFSGGRPAGRGIYAGGPSGNPFSRRYDAFVDDFMDFRLNELQTPESPDMLPV
                     RTRLHPDPTHPVPPAP"
     gene            3747..5255
                     /locus_tag="AC16YU_08965"
     CDS             3747..5255
                     /locus_tag="AC16YU_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6065396.1"
                     /translation="MTQAHDHRFSSRMGMLLSVLGIAVGTGNIWRFPRIAAQNAGDDG
                     AGAFLLVWLLFLFTWSVPLIIAEYALGRKGRMGVVGTFARVAGARFAWMGGFVGVVAT
                     AIMFYYSVVAGWCIYYFVKTIADPLPRSTEAATAIWESFQGGGWPIAFHALAMGLGAL
                     AVWKGIRSIERVNKILIPTLLLIVLIAVGRALTLDGAFDGIRFLFTPSWDTLARPRVW
                     LEALTQNAWDTGAGWGLILTYGAYMQARHGVVKNAFITGIGNNTVSLLAAIVIFGTVF
                     AVLGADMSKPEVLEVMRTSGPAATGLTFIWMPQLFDRMGFGSTIAVLFFLGLSFAAFS
                     SLISMIELATRAVVDAGVRRAQAVGAVCAIGFVMGIPSAVNLDLLGNQDFVWGVGLMI
                     SGAFVAFAVLRYGLGKFRTEIVDATEGDWKSGRTWEVLIGWAVPLMAVVLLAWWLFLA
                     ATVYAPDTWYDPLDPYSVMTCLVQWGAVIGLLLLVNRTLSRRTLEFAPPEEG"
     gene            5258..5752
                     /locus_tag="AC16YU_08970"
     CDS             5258..5752
                     /locus_tag="AC16YU_08970"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR6065397.1"
                     /translation="MDRPVRILFVCLGNICRSPLAEGVFRHLVREAGLEDRFEIDSAG
                     TGSWHVGEPPDPRTGKTAASHGVELDGRARQLTAGDLERYDLVLAMDRDNLHDILYLD
                     PTGSGSGHVRLFREFDPDPGDLQVPDPYYGGPEGFETVYRIVERTSRALLASLVAHYG
                     LRAP"
     gene            5757..6626
                     /locus_tag="AC16YU_08975"
     CDS             5757..6626
                     /locus_tag="AC16YU_08975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR6065398.1"
                     /translation="MDGDLKRLSRVVGSPVRRVRPVGGGCIAGASMVETDRGRRFLKW
                     GRGEIALALEAEADGLATLRSASGASGLRVPDVVAHAAEDGGEPALLVLEWIDEVPWT
                     DHLRERLGRGLAALHAVARPAYGHRRDNFIGRLPQENGELDDWPAFYLSRRLEPQARR
                     AREEAAWDPAWDRSYSALCRSLDDRLPRRPPPSALHGDLWSGNVMASAGGPVLIDPAV
                     YAGDRETDLAMARLFGGFGARFFAAYEEAWPLEAGWRSREPIYRLYHLLNHLNHFGSG
                     YAPAVERALRGET"
     gene            complement(6643..7335)
                     /locus_tag="AC16YU_08980"
     CDS             complement(6643..7335)
                     /locus_tag="AC16YU_08980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6065399.1"
                     /translation="MVLVLFDIDGTLIDAHGLGRRTFETALGEVAGRPVASGRVPFAG
                     RTDPAIAADMLRAAGISDDELPEVLSRGLAHFTALMLASSESAPVSRLPGVEAVLERL
                     DATHGVSLGLVTGNMEVSAFAKLRAAGLDRFFGFGAFGSDDRDRYALPGIALGRARSL
                     LGVDLDPASAVIVGDTPHDVGCGRGAGMKTVAVCTGPVSRAELVASEPDLLLDDLRDA
                     APFYRFLDLPPA"
     gene            complement(7347..7820)
                     /locus_tag="AC16YU_08985"
     CDS             complement(7347..7820)
                     /locus_tag="AC16YU_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR6065400.1"
                     /translation="MTWTDGAIPDCVVTDLRRFHDDRGWLAEIFRDDERPGGIEPAMS
                     YVSLTRAGVGRGPHAHRDQTDLFAFVDGRFRLFLWDAREGSRTRGTRQVMEFGRERPA
                     TVVVPPGVVHGYRNVGDSDALIVNCPDRLYAGRNRAEPVDEIRYEDDPDSPYRMD"
     gene            complement(7817..>8039)
                     /locus_tag="AC16YU_08990"
     CDS             complement(7817..>8039)
                     /locus_tag="AC16YU_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6065401.1"
                     /translation="FYDSQVFDVIRGLSPSRRGEYEVSDLSNWYASRGMLTHGTLAGW
                     WGDAGTLEGWHEANRLAVDLAYVELEISP"
BASE COUNT         1134 a         2752 c         2868 g         1285 t
ORIGIN      
        1 cgggccatga acgagagcga tgccgctgta tacctggtgg gtgcgtccgt tcagcgcccg
       61 cagcatgtcg gcagcgtgcg ccgcgtcgtc cggcttgccc aggatccggc cgtccaggac
      121 gacgaccgtg tccgcgccca gcacgaggcc ctccgggtgc cgccttgcca cgtcgaccgc
      181 cttttcgaga gagaggacct ccacgagatc ggacggctcc cggctgccgt catgccgttc
      241 gtccacgccc gagggatcga cctcgaaggg aattcccagc cgggacagca gggtacgccg
      301 gcgcggggaa gaggaggcga gaatgaaggg gcaggtgaat cgcatggcgg gcctcctaat
      361 cccgaccggg ggggcggagt tccggccagg gtccgccgcc cggtgggttc ggattcgccc
      421 ggaacggcat ttgacctgcg ggggatggcc ggggtacttt cggccgaccc aattcccagg
      481 cggtcattcc ttcatgatcc ccatcacggt ctgcaaccac aagggcggca ccggcaagac
      541 cacgacgagc ctgatgctgg ccgcggcgct cgggctgagc gggcggcgcg tgctcgccgt
      601 cgacctggat ccgcagggat tcctgtcccg cctcacgggg actccggaac cgccggccga
      661 gcgctcttcg cacgcgatgt tcgaggaagg ggcgccgttc gatccgggca gcgtcgtctc
      721 gctgccctcg ttcgacctcc tgccgtcgtc ttcacgcctg tcggccgcgc tcaggcgcct
      781 gaaccggccc gtcgacgtgc tctgggttcg cgagttcgcg agccggtacc ttgccgaata
      841 cgacttcgtg ctctacgaca ccgccgctgc ggtcacggtt ttcagcctga acgcgctcgt
      901 ggcgagccgg ctcgtggtga ttccggtgct tccggagtac cagccggtcg tcggtgcgga
      961 gcagacctgg caaacggcgt tgaccgtccg caagaagctc aacccgggcc tggagtcgcc
     1021 ggtattcctc ctcacgatgg tcgacgcccg aaagcgggag caccacctgt accggcagta
     1081 tctgcgggaa cgctacggtg catccgtcat ggagcccatc gtccggacca gtgcctcgct
     1141 cgcgggcacg cggcaggacg gccgcacggc cttcgacttc gatccgaggg ggagaggtgc
     1201 ggtcgactac gcgaacgtgg ccgacagcct gctcgcgcgc acggcgagcc tcgagctggt
     1261 cgccctggcc gccgaggcgt gatgcgggaa acgtccccga gatcggtggc gggtctcgcg
     1321 atcggcgtgc tggccatctt ggccacgccg tacgtgctcg gtctgtccgc cggtgggatg
     1381 ttccggatcg gacccatgct cgatccggtc gacggcctgt accggacggc ccggctggcc
     1441 gacgcggatc ctccttccgt cgcgaccgtg gcagggctgc agggtccggt cgaggtcgcc
     1501 tgggacgggc gtggcgttcc gcacatattt gcggagaatg atctcgatgc cgttcgtgcg
     1561 acaggttacg tgacggcccg tgaccgcttg ttccagatgg acttcctgag acggttcacg
     1621 tccggtcgcc tcgccgaggt gttcgggccg tccgcggtgt ccaccgaccg gtccatgcgg
     1681 cgcaccggca tcgaggccgc cgtgcgcatg aacgtggaag ccgcccgtgg cgacggccgg
     1741 cccacgtacg agttgttcgc tgccttcgcc gacggcgtga acgcctacct ggccgccgtc
     1801 gaggagcgcg acctgccttt cgagttccgg cttttcggct accgcccgga gcccttcacg
     1861 gcactcgacc cgggcctcat cggatggaac ctgttgtacg acctgacctt ccgggggacc
     1921 ccgagggtgg acgacgcggc cgtcgactcg ctgctggggc ccggtgcggg ggagatcctc
     1981 tttccgcgcg acaaccctct gagccgcccg atcgtcccgg cggaggaggc gcactggagg
     2041 aacggcgtcg accggtccgg ggcaaggggg ttcgcggtac gcctcccgtg gacggaggac
     2101 gagcgggaga cggagccgtt cgccgacctc acggccgggc tcgtggaggg caagggctcc
     2161 aacaactggg cggtgggtcc ccgccggtcg gctaccgggg cgccgatcct ggccggcgac
     2221 atgcacctgg cgctgtggct tccctcgatc tggtacgagc tgcacgtcgt gacgcccaca
     2281 atggacacgt acggcgtcac ggttcccggc gtgccactcc cgatcgaggc cttcaaccgg
     2341 tcggtggggt gggcgttcac gaacacgggc gcggaccagg tggaccatct ccttctcgtg
     2401 gaagactcca ccgggcggcg ataccggtac gaagacggat ggcgcgactt ccgcttcgag
     2461 gtggacaccc tgcgcgtgcg cggccgggat tccgtggtcg acaccgtgcg gtggacgcac
     2521 tggggacctg tcgtacaccg ccggggccgc gggatcgcca tccggtgggt ggcgcacgac
     2581 acgctccgga cgttcgacgc gctcctcgcc atgaaccgtg cccgcggcta cgatgagttc
     2641 gagcgggcca cccggctctg ggacgctccc atgcagaaca tcctggtggc cgacacggcg
     2701 ggcacgatag ccatccggtc cacgggccac ctgcccatcc gccgggacgg cggcggcgcc
     2761 ggctaccggg acggctcgtc gcccggatcc gactggacgg gccgcgtgcc cttcgacgac
     2821 cttccgcacg cggtcgatcc gtcgcgcggc tacctcacgt ccacgaacca gcagccgacc
     2881 gacggatcgt atccgtggta catggggcgg gactggcgat cgacctaccg atccatacgc
     2941 atcgacacgc tcctctcagg gcgtgaccgg cacgaccccg ggcagatcgc gtccttccag
     3001 gcggacgttc acgccgtgca gcgggactgg ttcgtgccgc tgctgggcgc ggcgggcacg
     3061 gtctcggccc gcgccgaaag gctgcgggcg atgctggagg cctggtcggg cgagaccggg
     3121 gtggagcgcc ccgagccgct cgtcatggac gtattcctgg gcgttctcga tgacctcgtc
     3181 tgggatgagc cgggcttcgc gaggattcgc cggccggccg acggcgtgct gtaccgcctg
     3241 ctgacgggcg cgctcgaggg gccgtggctg gaccgggtcg gcaccgaacg ggtcgaaacg
     3301 tcgggcgaca tcctagccga ggcgctcgaa ctcgcggccg actcgatcga ggccgcatac
     3361 ggtccggatc cggacggctg ggcctggggc cggcaccacc ggctcattct ccgtcatgtc
     3421 tcgcagacgc ccgtgctcca ggccctatgg cgtggccccg tgccgttccc cgggttcgag
     3481 tcgaccctct cgccaggagc cggtcgcaca gtgacccacg gcgcgagctg gcgcgtggtc
     3541 gtggacttct cgggcggccg gccggcgggc cgcggcatct acgccggcgg cccttcgggg
     3601 aatccgttca gccggcgcta cgatgcgttc gtcgacgatt tcatggactt ccgcctgaac
     3661 gaactccaga ccccggaatc tccggacatg ctgcccgtcc ggacccggct tcacccggat
     3721 cccacgcacc ccgtgccccc cgccccatga cccaggccca cgaccatcga ttctcgtccc
     3781 ggatgggcat gctgctgagc gtgctcggca tcgccgtcgg cacgggaaac atctggagat
     3841 tcccgcgcat cgccgcgcag aacgccgggg acgacggggc cggagccttc ctcctggtat
     3901 ggctcctgtt cctgttcacg tggagtgtgc cgctgatcat cgcggaatac gccctcggtc
     3961 ggaagggacg gatgggcgtc gtcgggacat tcgcccgggt ggcgggagcc cgattcgcat
     4021 ggatgggcgg attcgtcggg gtcgtggcca ccgccatcat gttctactac tccgtggttg
     4081 cgggatggtg catttactac ttcgtgaaga cgatcgccga tccgcttccc cggtccacgg
     4141 aagcggccac cgcgatctgg gagtcgttcc agggcggggg ctggccgatc gccttccacg
     4201 cgctggccat gggactgggc gcgctcgccg tgtggaaggg catccggtcg atcgagcgcg
     4261 tgaacaagat cctcataccg acgcttctcc tcatcgtgct gatcgcggtc ggccgggcgc
     4321 tcacgctcga cggggctttc gacgggatcc ggttcctgtt cactccgtct tgggacaccc
     4381 tcgcccggcc gcgcgtgtgg ctcgaggcgc tgacgcagaa tgcgtgggac accggggcgg
     4441 ggtggggcct gatcctgacg tacggtgcct acatgcaggc gcggcacggg gtcgtcaaga
     4501 acgccttcat cacgggcatc ggcaacaaca cggtgtcact gctcgcggcc atcgtgatct
     4561 tcggcaccgt gttcgccgtc ctcggggcgg acatgtccaa gcccgaagtg ctcgaggtca
     4621 tgcgtacgag cggtccggcg gcgaccggcc tcacattcat ctggatgccg cagctcttcg
     4681 accggatggg cttcgggtcc acgatcgccg tgctgttctt cctgggcctg tcgttcgcgg
     4741 ccttctcgtc gctgatctcg atgatcgagc tcgccacgag ggccgtggtg gacgccggcg
     4801 tgcgccgtgc gcaggcggtg ggcgccgtgt gcgcgatcgg cttcgtgatg ggcatcccgt
     4861 cggcggtcaa cctggatctc ctcggcaacc aggacttcgt ctggggggtg ggcctgatga
     4921 tctccggtgc cttcgtcgca ttcgcggtgc tccgctacgg cctgggcaag ttccggacgg
     4981 agatcgtcga cgccacggaa ggggactgga agtccggccg cacgtgggag gtgctcatcg
     5041 gctgggccgt gcccctgatg gccgtggtcc tgctcgcctg gtggctgttc ctggcggcca
     5101 cggtctacgc ccccgacacg tggtacgacc cgctggatcc ctattccgtg atgacgtgcc
     5161 tggttcagtg gggcgccgtg atcggcctgc tgctcctcgt caaccgcacg ctctcgagga
     5221 ggacgctcga gttcgctccc ccggaggagg gctgaccgtg gatcggcccg tccgcatcct
     5281 gttcgtgtgc ctgggcaaca tctgccggag tccactggcg gagggggtct tccgccacct
     5341 cgtccgcgag gcgggcctgg aggaccgctt cgagatcgat tccgccggca cgggcagctg
     5401 gcatgtcggc gagccccccg atccgcgcac cgggaagacg gcggcgtcgc atggtgtcga
     5461 gctcgacggc cgggcgaggc agctcacggc cggagacctg gagcgatatg acctggtcct
     5521 ggccatggat cgcgacaacc tgcacgacat actctacctg gacccgaccg gcagcggtag
     5581 cggacacgtc cggctcttcc gcgagttcga cccggatccg ggagatctgc aggtgcccga
     5641 tccctattac ggcggccctg agggcttcga gaccgtgtat cgcatcgtgg agcgaacgtc
     5701 gcgcgcgctg ctggcctcgc tcgtcgcgca ttacggcctc agggcgccct gaagtcgtgg
     5761 acggcgacct gaagcggctt tcccgtgtcg tggggagccc ggtgcggcgg gtccggccgg
     5821 tcggcggcgg gtgcatcgcg ggcgcgtcga tggtggaaac ggaccggggc cgccgcttcc
     5881 tcaagtgggg acggggagag atcgcccttg cgctggaggc cgaagcggac ggtctggcca
     5941 cgctgcgctc ggcgtcgggg gcgtcggggc tccgggttcc cgatgttgtg gcccatgccg
     6001 cggaggacgg cggcgagccg gccctcctgg tcctggagtg gatcgacgag gtcccgtgga
     6061 cggatcacct ccgggagcgc ctcggccgcg gcctggccgc gctgcacgcg gtcgcccggc
     6121 cggcatacgg tcaccggcgc gacaacttca tagggcgcct tccgcaggag aacggggagc
     6181 tcgacgactg gccggccttc tacctgtcca gacgtctgga gccccaggcg cggcgggcga
     6241 gagaggaagc ggcgtgggac ccggcatggg accggtcata ctccgccctc tgccggtcgc
     6301 tggacgaccg gcttccccgt cgccctccac catcggcgct tcacggcgac ctctggagcg
     6361 gaaacgtaat ggcatcggcc ggcgggccgg tgctcatcga tccggccgtg tacgccggtg
     6421 accgggagac ggacctggcg atggcccgcc tgttcggcgg gttcggcgcc cggttcttcg
     6481 cggcctacga ggaggcgtgg ccgctggagg ccggctggcg gtcgcgcgag ccgatctacc
     6541 ggctctacca cctactcaat cacctgaacc acttcggctc ggggtacgcg ccggccgtcg
     6601 agcgggcgct gcgcggcgaa acgtgaacgg cggccggcag gctcaagccg gcggaaggtc
     6661 caggaatcgg tagaacggcg ccgcgtcccg caggtcgtcg agcagcagat cgggctcgga
     6721 ggccacgagc tccgcgcgcg acaccgggcc cgtgcacacg gcgacggtct tcatgccggc
     6781 tccccggccg cagccgacgt cgtggggcgt gtcccccacg atgacggcgg aggcgggatc
     6841 caggtccacg ccgagcaggg atcgcgcacg gccgagcgcg atccccggaa gggcgtaccg
     6901 gtcacggtcg tcgctcccga acgccccgaa gccgaagaat cggtccaggc cggccgcccg
     6961 cagcttggcg aaggcggaga cctccatgtt gcccgtcacc agtccgagcg agacgccgtg
     7021 cgtggcgtcc aggcgctcga gcaccgcctc gaccccgggc agccgcgaca cgggggccga
     7081 ttcgctcgag gcgagcatga gggccgtgaa gtgggcgagc ccgcgggaaa ggacttcggg
     7141 gagctcgtcg tcggatatcc cggccgcgcg aagcatgtcg gcggcgatcg cgggatccgt
     7201 ccgtccggcg aacgggacgc ggccggaggc gacggggcga cccgcgactt cccccagggc
     7261 ggtctcgaaa gtccggcggc ccagcccgtg cgcatcgatg agcgttccgt cgatatcgaa
     7321 gaggaccagc accacgggcg gcggggtcag tccatccggt acggcgagtc cggatcgtct
     7381 tcgtaccgga tctcgtcgac cggctcggcg cggttccgtc ccgcgtacag acggtccgga
     7441 cagttcacga tgagggcgtc cgagtcgccc acgttgcggt aaccgtgcac cacgcccggc
     7501 ggaaccacga cggtcgcagg gcgctcgcga ccgaactcca tgacctgcct cgtgccgcgg
     7561 gtccgggagc cctccctcgc gtcccacaga aacagccgga accgcccgtc gacgaacgcg
     7621 aacaggtcgg tctggtcgcg gtgggcgtgg gggccccggc cgacgcccgc ccgcgtaagg
     7681 gagacgtacg acatggccgg ctcgatcccc ccggggcgct cgtcgtcgcg aaagatctcg
     7741 gcgagccagc cgcgatcgtc gtggaagcgc cgcaggtcgg tgacgacaca atccggtatg
     7801 gcgccgtcgg tccaggtcac ggagagatct cgagttcgac gtacgcgagg tccacggcca
     7861 gacggttcgc ctcgtgccag ccttcgaggg ttccggcatc tccccaccag ccggcgagtg
     7921 tgccgtgggt gagcatgccg cgcgatgcgt accagttgct cagatcgctc acctcgtact
     7981 cgccgcggcg cgacggagaa aggccccgga tcacgtcgaa gacctggctg tcgtagaag
//
LOCUS       DBNHQZ010000335         6663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2942888, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000335 DBNHQZ010000000
VERSION     DBNHQZ010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2942888"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1414)
                     /locus_tag="AC16YU_08995"
     CDS             complement(317..1414)
                     /locus_tag="AC16YU_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6065402.1"
                     /translation="MAHTRRDFLKTAALAGGVLLSPVRPHRRRALRILVLGGTSFIGP
                     FQVRYALDRGHEVTLFNRGRTNIDLFPGLEKLRGDRSGDLEALRGRQWDVVIDNSATN
                     PEWVRLSARFLRDSVERYFYVSSRSAYEHFRQVPMTASAPTYTHESAGVDPSTPGADL
                     PYGLAKAQSEREATSVFGRRATILRPGLIIGPRDETDRFTYWPVRIHRGGEILAPGDG
                     TDGVQIIDARDLSEWMIRLAEDGRGGTFNAVGPAWGRSFAELLHGIRAVTTAETRFTW
                     VDTDFLVDRGVRPYSDLPVWMPARGDDVGFARFDLSREVAAGLTFRSLAETTSDTLEY
                     HFSRPAARQEALRAGMTAERERELLREWGRR"
     gene            complement(1535..2002)
                     /locus_tag="AC16YU_09000"
     CDS             complement(1535..2002)
                     /locus_tag="AC16YU_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065403.1"
                     /translation="MRSAIWHDVHRLHGEGLSTDSIAKALGKPPYRVLRLLTESGALD
                     RQYEYGIRDWRLLEDYREGLTQREICDRYHVQPSLLQKILRIHGEPIRPRKPALEPEL
                     VRAVVADYLDSDLTREEICAEHSITNAVLERILEEEDIPRRTPPAGGRGRRGF"
     gene            complement(2091..2164)
                     /locus_tag="AC16YU_09005"
     tRNA            complement(2091..2164)
                     /locus_tag="AC16YU_09005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2128..2130),aa:Val,seq:gac)
     gene            2393..2800
                     /locus_tag="AC16YU_09010"
     CDS             2393..2800
                     /locus_tag="AC16YU_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065404.1"
                     /translation="MKLQVTATKTLDTRPPGGDGAATGTVRHAHPVPVARRFPPVIQV
                     LTVDDPSAEQQLAARANESYREGKALRDICTDVHRLARSIGYDATIGVQEVFSTSMPS
                     FPGQTVISALLMLDLRPLWSSGEGARPSLRSAG"
     gene            complement(2925..3599)
                     /locus_tag="AC16YU_09015"
     CDS             complement(2925..3599)
                     /locus_tag="AC16YU_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065405.1"
                     /translation="MAPGPRLALPFAALLLLAAPAAAQPDPASGIRLENPSAGRAFAM
                     SLVLPGWGHRYANGGEWGGSGTFFALADAFLWTGLVGTSWQRGHVIRSYETLAVTGAG
                     ADMDGKDRLFSLRLASFRSSDEFREVALRTRAWEDLSYVDDPAYWWSWTDEADYLRFR
                     DLREDGESLGRRRTAIIATLVANRLISGFLAARSARSRGGADFALALRPTRSGSIPAV
                     DLALRF"
     gene            3971..5101
                     /locus_tag="AC16YU_09020"
     CDS             3971..5101
                     /locus_tag="AC16YU_09020"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR6065406.1"
                     /translation="MVVAMVIISVVAFVVVDLIIRFVLRRVTAARVRKEREEALETGL
                     RLAFSEETPSLKRVTVEEPKARILAVDDEAIILDSFRKILVLEGFAVDTVETGPEALN
                     LVRAGDYDFVFTDLKMPDMDGVEVVKGVKHLRPDVDVVVITGFATIETAVETMKYGAM
                     DYVQKPFTAEELAQFANKSLIRRQDRIERALRPHVHLVTPTAGSSASPHEFNVPSGLF
                     VSEDHIWVGLFANGLLQAGVDDFAQKIFGPVKDLDLPAPGTTVARGEPLVALVRNGHR
                     IQLPAPVSGRVVAVNEDLDHRPEMVNLRPYEAGWVCWIEPSDLPAELPTLRIGANATA
                     WYEDEIERYEALRKGGDAAAATESKTEEEATWDAIERTLVRA"
     gene            5098..5610
                     /locus_tag="AC16YU_09025"
     CDS             5098..5610
                     /locus_tag="AC16YU_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6065407.1"
                     /translation="MRALRAPGWTLMAGLVVVGLVATPGFLRMQDTDDAAEEPVAASV
                     RPGDPGSYGGWGEANFPHDFHIDDLEIACEDCHHETNAARLQMPHDDYFDDFWIDCTI
                     CHKTDGEVSLDPQACSDCHPSNPRNSADETLSAKVVIHQNCWECHDRGTGAEAAENCS
                     FCHDGPSFEE"
     gene            5630..6535
                     /locus_tag="AC16YU_09030"
     CDS             5630..6535
                     /locus_tag="AC16YU_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6065408.1"
                     /translation="MDRRNFLKTAGVACATVAGAKSATAQEHDTDPEEFVGVLVDTTR
                     CIGCRSCEIGCALAHNLPVPDVASDGALEAERKTSETAWTVVNRYETDAGEVFVKKQC
                     MHCWQPACAAACLTNAMKKTESGPVIWESDKCMGCRYCMISCPFEMPKAEYDEWNPRI
                     QKCNFCFERLQEGMKPACVESCPTDALMFGTKRELMEIARTRIYAHPDRYVHQVYGEF
                     EAGGTGWLYLAAAPFEQLGLPTNLGTTPYPEFTRDFLYAVPVVFFGVPALLYGFSLLA
                     DRKDEIKGPHGAILPAPTIESESGD"
BASE COUNT         1068 a         2267 c         2394 g          934 t
ORIGIN      
        1 cgcgcatgga ggagcagggg caccgggtgc tgtactggga gaacaccgag ggcggccacg
       61 gggcagccgt cacgaacgag cagcgggcct acagggaggc actcgtatac gcgtacctct
      121 accgccagct gttcggcgcc ggaggggccg gctgactcgg taacgacggt cgtcgacgcg
      181 aatctggcgg aggggaccgc cgctctccgg taatcctccc tcccggtcta cgacacattg
      241 gcccgtcgac tcagccagcc catggacgag attctgaagg cgggtcgcca cgtgatcgac
      301 ttcgtcacgc ccggcctcac cgccgccccc actccctgag cagctccctc tcccgctccg
      361 ccgtcatccc cgcccggagc gcctcctgcc tcgccgccgg ccgcgagaag tggtactcga
      421 gcgtgtccga ggtcgtctcg gcgagcgacc ggaacgtcag gccggccgcg acctcgcgcg
      481 agaggtcgaa gcgcgcgaag cccacgtcgt cgccgcgggc gggcatccac acgggcaggt
      541 ccgagtacgg acgcacgccc cggtccacca ggaagtccgt gtccacccag gtgaagcggg
      601 tctccgccgt cgtgacggcg cggatgccgt gcaggagctc cgcgaaggag cgtccccagg
      661 ccgggcccac cgcgttgaat gtgccgccgc ggccatcctc ggccaggcgg atcatccact
      721 ccgagaggtc gcgggcgtcg atgatctgga cgccgtccgt gccgtcgccc ggcgcgagga
      781 tctcgccgcc ccggtggatg cggaccggcc agtaggtgaa gcggtcggtc tcgtcgcgcg
      841 ggccgatgat gaggccgggg cggaggatgg tcgcgcgccg gccgaagacg ctggtcgcct
      901 cgcgctcgga ctgggccttc gcgaggccgt agggcaggtc cgcgccgggc gtcgaggggt
      961 ccacgccggc cgactcgtgc gtgtaggtcg gcgccgacgc cgtcatgggc acctgccgga
     1021 agtgctcgta cgccgaacgc gacgacacgt agaaatacct ctccacgctg tcccgcagga
     1081 agcgggccga gagccgcacc cactccgggt tcgtggccga gttgtcgatg acgacgtccc
     1141 actgccgtcc gcgaagggcc tcgaggtccc cgctgcggtc gccgcgcagt ttctcgagcc
     1201 cggggaagag gtcgatgttg gtgcggccgc ggttgaagag ggtcacctcg tgcccgcggt
     1261 ccagcgcgta ccgcacctgg aaggggccga tgaagcttgt gcccccgaga acgaggatcc
     1321 gcagcgctcg gcgccgatgc gggcggaccg gggagaggag cacgccgccg gccagggcgg
     1381 cggtcttcag gaaatcgcgg cgggtgtggg ccatggtggg cgcggcaggg tggcactcgc
     1441 ggccaacata ctgtacgtcg gcgggatcag ggctcctgcc gcacctcccg ccggcccacc
     1501 ggccggaacg ctcggcgcgg gcgcggcaca gcggtcagaa tccccggcga ccgcgacccc
     1561 ccgcgggcgg cgtccggcgc gggatgtcct cctcctcgag gatgcgctcg aggaccgcat
     1621 tcgtgatcga gtgctcggcg cagatctcct cgcgcgtcag gtcggaatcg aggtagtcgg
     1681 cgacgaccgc gcgcacgagc tcgggctcga gcgcgggctt gcgcggccgg atgggttcgc
     1741 cgtggatgcg caggatcttc tgcaaaagac tgggctggac gtggtagcgg tcgcagatct
     1801 cgcgctgggt cagcccctcg cggtagtcct cgagcagccg ccagtcacgg atgccgtact
     1861 cgtactggcg gtccagcgcg cccgactcgg tgagcagccg gagcacgcgg tacgggggct
     1921 tgccgagagc cttggcgatg gagtcggtgg atagaccctc gccgtgcagc cggtggacgt
     1981 cgtgccagat cgcgcttcgc atggggcggg gaaacgcggc aggccctgcg gggattcggg
     2041 gccgcgtgaa atgacaaacc ccgaccggag ggctccgatc ggggcttccg tgggtcctag
     2101 tggactcgaa ccaccgacct ctacgatgtc aacgtagcgc tctaaccaac tgagctaagg
     2161 acccgtccaa gactatcaag ataccgcggc ttcgcgaccc cggtcaagcg cggcgccccg
     2221 attcgggccg cccccggggc gccgcagcag gccgcagcag gccgcggcag gccgcggcag
     2281 ggcgccgcag aagggcacgg cagggcgccg cggcacggcg cgccagccgc ggcagccagg
     2341 acttggcccg cttttggctt ccctcccccg acgcaccctc agcgaggtcg tcatgaagct
     2401 ccaggttacc gcgacgaaaa cgttggatac gcgtcctccg ggaggggacg gggcggccac
     2461 aggcacggtc cggcatgcgc atccggtgcc cgtggcgcgg cgttttccgc cggttatcca
     2521 ggtcctgacg gttgacgacc cgtcggcgga gcaacagctg gccgcccgtg cgaacgagtc
     2581 ctaccgcgag ggaaaggccc tgcgcgacat ctgcacggac gtgcaccggc tcgcgcggtc
     2641 catcggctac gacgcgacga tcggcgtcca ggaggtcttt tcgacgtcca tgccgtcgtt
     2701 tcccggccag acggtgatct cggcgctcct catgctggac ctgcggcccc tctggtcgag
     2761 cggggagggc gcgaggcctt cgctgcgcag cgccgggtag cggcgccggg taggaaaggt
     2821 ctgccgcgcg ataggaaggg ctttccgcgg ccgagccgtg ctccggcggg ccgccgccgg
     2881 ggaagtccgg gcgcggaagc gcgcctcgat tccggaccca caggtcagaa tctgagcgcc
     2941 aggtccacgg ccgggatcga tccgctccgc gtgggacgga gcgccagcgc gaagtcggcg
     3001 ccgccgcggc tgcgggcgga gcgcgccgcg aggaagcccg agatcagccg gttggccacg
     3061 agggtcgcga tgatcgccgt gcgccggcgc ccgagcgatt cgccgtcctc gcgcaggtcg
     3121 cggaatcgca ggtagtccgc ctcgtcggtc cacgaccacc agtaggcggg atcgtccacg
     3181 taggagaggt cctcccacgc gcgggtgcgc agggccacct cgcggaactc gtccgacgag
     3241 cggaagctcg cgagccgcag gctgaagagc cggtccttcc cgtccatgtc ggctccggcg
     3301 cccgtgacgg cgagcgtctc gtagctccgg atcacgtggc cgcgctgcca ggacgtgccg
     3361 acgagcccgg tccagaggaa ggcgtccgcg agcgcgaaga aggtcccgct gccgccccac
     3421 tctccgccgt tcgcgtaccg gtggccccag ccgggcagca cgagcgacat ggcaaatgcc
     3481 cgcccggccg agggattctc gaggcggatg ccggacgccg ggtccggctg ggccgcggcc
     3541 ggcgccgcca ggaggagcag cgcggcgaag ggcagggcaa ggcgcggacc gggagccatc
     3601 gggagcggag aagatgcgtc ctcctcaacg aatcggggcg cccgtggatc ctgcgggcgt
     3661 gcgtgcaggg gaaaccctcg gagggcacgc gccgaacacg ccaacgtgcc ggggcacgcc
     3721 gtcccccgcg gcggtcctcc gcccgatgcc aggacgggca tttcatggcg acccgtgcgc
     3781 accccggcac ccacattccg agccgtcaac ggactacatg ccgcgtttcg ctgcgggcgt
     3841 accatggcgg cggtcggagc atgggaaacc gggcgcttgc ccggcgtgcc cgaccgtgaa
     3901 atactgatca aaccgcggca aatggagcca gtgctccgcg agccgcgcac ataccccggg
     3961 ggtacgcgtc atggttgtcg caatggtcat catatcggtc gtcgccttcg tggtcgtcga
     4021 cctgatcatc cggttcgtcc tgcgccgggt gacggccgcg cgcgtccgga aggagcgcga
     4081 ggaggcgctc gagaccgggc tgcgcctggc tttctcggag gagactccga gcctgaagcg
     4141 ggtgacggtt gaagagccga aggcccggat cctcgcagtg gacgacgagg cgatcatcct
     4201 cgactcgttc cgcaagatcc tcgtgctcga aggctttgcg gtggacacgg tcgagacggg
     4261 cccggaggcc ctgaacctgg tccgcgcggg ggactacgac ttcgtcttca ccgacctgaa
     4321 gatgcccgac atggacgggg tcgaggtcgt gaagggtgtg aagcacctcc gtcccgacgt
     4381 cgacgtcgtc gtgatcacgg gcttcgccac gatcgagacg gccgtcgaga cgatgaagta
     4441 cggggcgatg gactacgtcc agaagccgtt cacggccgag gagctcgccc agttcgccaa
     4501 caagtcgctc atccggcggc aggatcggat cgagcgagca ctccggccgc acgtccacct
     4561 cgtgacgccg accgcgggca gctccgcgtc gccgcacgag ttcaacgtgc cctcgggtct
     4621 cttcgtgtcg gaggaccaca tctgggtcgg cctcttcgcg aacggcctgc tgcaggccgg
     4681 cgtggacgac ttcgcccaga agatcttcgg cccggtcaag gacctggacc tgccggcacc
     4741 cggcacgacc gtggcccggg gcgaacccct ggtggcgctc gtccggaacg gccaccgcat
     4801 ccagctgccg gcgccggtct cgggacgcgt cgtcgcggtg aacgaggatc tggaccaccg
     4861 gcccgagatg gtgaacctca ggccttacga ggcgggctgg gtgtgctgga tcgagccgtc
     4921 cgacctgccg gccgagctgc cgacgctccg catcggcgcg aacgcgacgg cctggtacga
     4981 ggacgagatc gagcgctacg aggcgctgcg caagggtggc gatgccgctg ccgcaacgga
     5041 gtccaagacc gaggaggaag caacatggga cgcgatagag cgcacgctcg tccgcgcatg
     5101 agggcgctgc gtgcaccggg atggacgctc atggccggcc tggtggtcgt gggcctcgtg
     5161 gccacgcccg ggttcctgcg gatgcaggac acggacgatg ccgccgagga gccggtcgcc
     5221 gcgagcgtgc ggccgggtga tcccggcagc tacgggggct ggggcgaggc gaacttcccg
     5281 cacgatttcc acatcgacga cctggagatc gcgtgcgagg actgccacca cgagacgaac
     5341 gccgcgcggc tccagatgcc gcacgacgac tacttcgacg acttctggat cgactgcacg
     5401 atctgccaca agacggacgg cgaggtgtcg ctcgatccgc aggcgtgcag cgactgccac
     5461 ccgtcgaacc cccgaaacag cgccgacgag actctctccg ccaaggtggt cattcaccag
     5521 aactgctggg agtgccacga ccgcgggacc ggggccgagg cggccgagaa ctgctcattc
     5581 tgccacgacg ggccgtcctt cgaggagtga cgcccggacc aggtcaacca tggacagacg
     5641 taattttctc aagaccgcgg gagtggcgtg cgccacggtg gccggcgcga agagcgcgac
     5701 cgcccaggag catgacaccg atcccgagga attcgtcggc gtgctcgtcg acaccacgcg
     5761 gtgcatcgga tgtcgctcgt gcgagatcgg gtgcgccctg gcccacaacc tgcccgttcc
     5821 cgacgtcgcc tcggacggtg cgctcgaggc cgagcgcaag accagcgaga ccgcctggac
     5881 ggtcgtgaac cgctacgaga ccgacgcggg cgaggtgttc gtcaagaagc agtgcatgca
     5941 ctgctggcag cccgcctgcg cggcggcgtg ccttacgaac gcgatgaaga agaccgagtc
     6001 ggggccggtc atctgggagt cggacaagtg catggggtgc cggtactgca tgatctcgtg
     6061 cccgttcgag atgcccaagg ccgagtacga cgagtggaac ccgcgcatcc agaagtgcaa
     6121 cttctgcttt gagcgcctgc aggagggcat gaagccggcc tgcgtcgagt cgtgcccgac
     6181 ggatgccctg atgttcggga ccaagcgcga gctcatggag atcgcgcgca cgcgcatcta
     6241 cgcgcacccg gaccgctacg tgcaccaggt gtacggcgag ttcgaggccg gaggcacggg
     6301 atggctgtac ctcgcggccg cgccgttcga gcagctgggc ctgcccacga acctgggcac
     6361 taccccgtac cccgagttca cgcgggactt cctgtacgcc gtgccggtcg tgttcttcgg
     6421 ggtgccggcg ctgctctacg gcttcagcct gctcgccgac cgcaaggacg agatcaaggg
     6481 accccacggg gcgatcctgc cggccccgac catcgaaagc gaaagtggag actgacatgg
     6541 caaacggatc gacttcggtg ccggcggcgg gacgaattcg cttcttcctg tcggagctca
     6601 agccccgggg gcgcatggcg acccccttca acatgatctc gatcccgatc atcctggtcg
     6661 ggt
//
LOCUS       DBNHQZ010000336         3551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1456066, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000336 DBNHQZ010000000
VERSION     DBNHQZ010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1456066"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="AC16YU_09035"
     CDS             <1..1112
                     /locus_tag="AC16YU_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR6065409.1"
                     /translation="EGMRMTIIKDELLEMKEKYADPRRTEIDYSGGDDIIIEDLIEDD
                     QMVVTVSHQGLVKRTSVSEYRQQGRGGVGKRGLAMRDEDYVEHLFVSRNHDYLLFYTD
                     HGRCYWLRVFEIPEGGRQSKGRSIRNLIQITPEDRIRAILAVSKPDFRDSGFLNGHFV
                     LMATRRGQVKKTPLEAFSRPRVDGIIAISIDEGDELLEAKLTAGDANVILANSRGLSI
                     RFRESNVRPMGRNTRGVRGMALSGEDEVVGMVIIENGEQDLLAISSRGYGKRSAIDDY
                     RVQTRGGKGIITMKATQRTGNLVAIKGVLESDDLMISTVNGIMIRMHVADISTLGRNT
                     QGVRLINLKGDDAIADVTRVVPDDDEDEPVEAGDD"
     gene            1186..1950
                     /locus_tag="AC16YU_09040"
     CDS             1186..1950
                     /locus_tag="AC16YU_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like protein"
                     /protein_id="HIR6065410.1"
                     /translation="MRQILLPALVTLLFAPAAAGQSVTAESVLRAHIEAAGGEEAFRA
                     VTSRYARGEVSLDTPVGPVILDFEQSVAFPGHALVRQTAVTAPPEIPAEMLKQTIFLT
                     PDGGWIEAAQFGGRVAFADLPEAQRGAFEQQRRSLAGVSEELELLGNDTLQVTLGPPD
                     TLNGVPVHVLEIPGDFPSRRMYSAESGLLVGAEATGPMGRSTQYYSDYRTVSGIRTPF
                     AVEMNQGGQAMTVSFSVIAFDRGLTPDSLRAASEEN"
     gene            complement(2011..3357)
                     /gene="lat"
                     /locus_tag="AC16YU_09045"
     CDS             complement(2011..3357)
                     /gene="lat"
                     /locus_tag="AC16YU_09045"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine:2-oxoglutarate
                     6-transaminase activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HIR6065411.1"
                     /translation="MEAVTGHVITPDKVAEIYLKHTNARGMMPMVLDMENSRGVRLVD
                     EASGRTYLDFFGFYASSAVGMNHPKMESDEEFLLRLKTAAMNKITNSDIRTVLMARFL
                     DTFDRVGIPDYLPYAFFVAGGALAVENALKTAFDWKVRKNFEKGYRREVGQQVLHFDQ
                     AFHGRSGYTLSLTNTADPRKTMYFPKFDWPRALNPKMVFPVEEHLEDIEARERRALAQ
                     VKTHFHERRDDIACVIIEPIQGEGGDNHFRREFLQALKDLCLENDTLLVFDEVQTGVG
                     ITGSFWAHQGIGVEPDILAFGKKTQVCGILAGRRLDEVEDHVFKLGSRINSTWGGNLV
                     DMVRFDRILEIIEEDGLVSHAAEAGAYLQQRLRALAGRHPSVSNVRGRGLFCAIDTPS
                     GDYRARLLKRCFEDGLVILGCGASSVRFRTPLNVTTDELDEGLRIVESAIAAIDAE"
BASE COUNT          596 a         1227 c         1156 g          572 t
ORIGIN      
        1 acgaggggat gcggatgacc atcatcaagg acgagctcct cgagatgaag gagaagtacg
       61 ccgacccccg gcgtaccgag atcgactatt cgggtgggga cgacatcatc atcgaggacc
      121 tgatcgagga cgaccagatg gtcgtcacgg tctcgcacca ggggctcgtc aagcgcacca
      181 gcgtgtcgga gtaccggcag cagggccggg gcggcgtcgg caagcgcggc ctcgccatgc
      241 gcgacgagga ctacgtggag cacctgttcg tgtcgcgaaa ccacgactac ctgctcttct
      301 acaccgacca cgggcggtgc tactggctgc gcgtgttcga gatacccgag ggcggccggc
      361 agagcaaggg ccgttcgatc cggaacctga tccagatcac gcccgaggac cgcatccggg
      421 cgatactggc cgtgtccaag cccgatttca gggactccgg gttcctgaac ggccatttcg
      481 tcctgatggc cacgcgtcgc gggcaggtca agaagacccc gctcgaggcc ttctcccgcc
      541 cgcgggtgga cggcatcatc gccatatcga tcgacgaggg cgacgagctg ctcgaggcga
      601 agctgacggc gggcgacgcc aacgtgatcc tcgctaacag ccgcggcctc tcgatccgct
      661 tccgcgagtc gaacgtccgg cccatgggcc ggaacacccg cggagtgcgc ggcatggcgc
      721 tctcgggcga ggacgaggtc gtgggcatgg tgatcatcga gaacggcgag caggacctcc
      781 tggcgatctc ctcgcgcggg tacggcaagc gatcggccat cgacgactac cgcgtgcaga
      841 cgcgcggagg gaagggcatc atcaccatga aggccaccca gcgcaccggc aacctcgtcg
      901 cgatcaaggg agtcctcgag tcggacgacc tcatgatctc gaccgtgaac ggcatcatga
      961 tccggatgca cgtcgcggac atctccacgc tcggccgcaa cacgcagggc gtccgcctca
     1021 tcaaccttaa gggcgacgac gcgatcgccg acgtcacccg cgtcgtgccg gacgacgacg
     1081 aagacgaacc ggtcgaagcg ggcgacgact gagcgaccac tacccattcc ccactcccgg
     1141 gagatcggcc agccgacctc ccgaccaccg aacaggtacc gcaccatgcg ccagattctg
     1201 ctcccggctc tcgtcaccct ccttttcgca cccgccgccg ccggccagtc cgtgaccgct
     1261 gaaagcgtgc ttcgggccca catcgaggcc gcgggcggcg aggaggcctt ccgcgccgtc
     1321 accagccgct atgcccgggg ggaggtgtcg ctcgacaccc cggtcggccc cgtcatcctg
     1381 gacttcgaac agtcggtcgc ctttccgggc cacgcgctcg tccgccagac ggccgtcacc
     1441 gccccgcccg aaattccggc cgagatgctg aagcagacca ttttcctgac ccccgacggc
     1501 ggatggatcg aggcggccca gttcggtgga cgcgtcgcgt tcgccgacct tccggaggcc
     1561 cagcggggcg ccttcgagca gcagcgccgc tcactcgccg gcgtctccga ggagctggag
     1621 cttctcggaa acgacacgct ccaggtgacg ctcggcccgc cggacacgct gaacggcgtt
     1681 cccgtccacg ttctcgagat tccgggcgat tttccgagcc gccgcatgta ctcggccgag
     1741 tcggggctgc tcgtcggggc cgaggccacg ggaccgatgg gccggagcac ccagtactac
     1801 tccgactacc gtaccgtgtc gggcatccgt acgcccttcg cggtggaaat gaaccagggc
     1861 gggcaggcga tgacggtctc cttctcggtg atcgccttcg accgcggcct gacccccgat
     1921 tcgctgcgcg cggcctccga ggagaactga gccggcccgc accggtatgg agcggccggg
     1981 cgagggctgc ccgcatccgc gcggacgccc ctattcggcg tcgatcgccg cgatggcgga
     2041 ctccacgatc cggagcccct cgtcgagctc gtcggtcgtg acgttgagcg gcgtccggaa
     2101 gcggaccgaa ctcgcgccgc agcccaggat caccaggccg tcttcgaagc accgcttgag
     2161 gagcctcgcc cggtagtcgc ccgacggcgt gtcgatcgcg cagaagaggc cgcggccgcg
     2221 gacgttcgag acgctcgggt ggcggccggc cagcgcgcgc aaccgctgct gaaggtaggc
     2281 gccggcttcc gcggcatggg acacgaggcc gtcctcctcg atgatctcca ggatccggtc
     2341 gaaccggacc atgtcgacca ggttgccccc ccacgtcgag ttgatccggc tgcccagctt
     2401 gaacacgtgg tcctcgacct cgtccaggcg ccggcccgcg agaatgccgc agacctgcgt
     2461 cttcttgccg aaggccaaga tgtcgggctc gacgccgatc ccctggtggg cccagaacga
     2521 tccggtgatg cccacgccgg tctgtacctc gtcgaacacg aggagcgtgt cgttctcgag
     2581 gcagaggtcc ttgagcgcct ggaggaactc ccggcggaaa tggttgtcgc caccctcgcc
     2641 ctggatgggc tcgatgatca cgcacgcgat atcgtcgcgg cgctcgtgga agtgggtctt
     2701 gacctgggcc agcgccctgc gctcccgggc ctcgatgtcc tcgagatgct cctcgaccgg
     2761 gaagaccatc ttcgggttga gcgcccgcgg ccagtcgaac ttcgggaagt acatggtctt
     2821 ccgcggatcg gccgtgttcg tgagcgagag cgtgtacccc gatcggccgt ggaacgcctg
     2881 gtcgaaatgc aggacctgct gtccgacctc ccgccggtat cccttctcga agttcttgcg
     2941 caccttccag tcgaaggccg tcttgagggc attctcgacc gcgagcgcgc cgcccgcgac
     3001 gaaaaaggcg tatgggaggt agtcggggat tcccacccgg tcgaacgtgt ccaggaaccg
     3061 ggccatgagc accgtccgga tgtccgagtt cgtgatcttg ttcatcgcgg cggtcttgag
     3121 ccgcagcagg aactcctcgt cggattccat cttcggatgg ttcatcccca ccgcggagga
     3181 ggcgtagaac ccgaagaagt cgaggtacgt gcgacccgac gcctcgtcca cgagccgcac
     3241 tccgcggctg ttctccatgt ccagcaccat gggcatcatg cccctggcat tggtatgctt
     3301 caggtagatc tcggcgacct tgtcgggagt aatcacgtgc ccggtaactg cttccatgtc
     3361 cgtcccattg cgtatgctgt gcctggtcga tccgcgcgca cgcgtgccct cgagggaccg
     3421 cgtgagcagc cttgaagata cgatccgggg cccttccggc cgccggtatc ggcgattctt
     3481 caccccgcga cgggggatca tttgcaccgg gggccggtag gtttcggccc gacacgacca
     3541 cgcgccatgg c
//
LOCUS       DBNHQZ010000337         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_777782, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000337 DBNHQZ010000000
VERSION     DBNHQZ010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_777782"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="AC16YU_09050"
     CDS             complement(<1..288)
                     /locus_tag="AC16YU_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR6065412.1"
                     /translation="MSSAPAFRASAALVLALFLSASAVRAQEAPVPVESGPLDNGKMW
                     LFENPPLDYLEATYGFRPDAAWLERARLSVLRVPGCSASFVSPNGLVVTNHH"
     gene            396..2726
                     /locus_tag="AC16YU_09055"
     CDS             396..2726
                     /locus_tag="AC16YU_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR6065413.1"
                     /translation="MSAETPEAARPAAATNFIRQIIENDLKSGSTGGRVVTRFPPEPN
                     GYPHIGHAKAICLNFDIAADYGGTCNLRFDDTNPETEDMEYVRAMERDIRWLGYDWGE
                     NRFFASDYFEELYDLAVRLIRGGKAYVDSQSTDEIRLTRGTVTEPGRPSPYRDRSVDE
                     NLDLFRRMKEGEFPDGAHVLRARIDMASPNMLMRDPVLYRIKHAEHYRRGNDWCIYPL
                     YDFAHPLSDAIEGITHSLCTLEFDVHRPLYDWLVDALFAPPRPHQYESARLNLDYTVM
                     SKRKLLQLVKEGHVAGWDDPRMPTISGMRRRGYTPESIRRFCALIGVAKADNRVDLSL
                     LEYAVRDDLNHRAPRVMVVLDPLEVELTNWPADRLEWLDAPYWPHDVPKEGSRQVPFG
                     RTLLIERDDFMEDPPKRFHRLSPGEEVRLRYGYIIRCDEVVKDAAGRVQRLRCSVDPD
                     TRSGETGGRRVKGTIHWVEATHAVPVEVRLYDRLFTVPDPEDVPEGESFTDRLNPRSG
                     DLVPAAMAEHSLRAAGPGDRFQFERQGYFFVDPEDSAEGRPVFNRTVTLRDTWAKIAS
                     DGGGRPAAKKTRDAAPPAPPAPSAPPVPAMTSKQEARARELAERYGIAFEDAALLATD
                     PDLRAFFEAAAGRSKHPRPIANWVVHDLQRERKELPLAEIALTPEHLAELVDLVEDGT
                     LSSRLAKEVFADVLSTGRSPRDVVDERGLVQIDDRDHLAAVAREVVAAFPDRADAWRG
                     GKAGLIGFFVGQVMQRTGGKANPKLTRELLEEALAG"
     gene            complement(2736..>3358)
                     /locus_tag="AC16YU_09060"
     CDS             complement(2736..>3358)
                     /locus_tag="AC16YU_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6065414.1"
                     /translation="DCTTRSTVDARRMHHLTASPDGAHVAYVHRELVYERSSGQYVAD
                     STFMIAGPDGSDERPVVGFRYRPERFAWRPDGTELAYDVQNPDRPGQRLVSIFDVASG
                     RSTYLHAPTADGPSEFDPHWSPDGSRILYRQTAAGATVLAVRTFADPYAVVLDGSEGA
                     EPAGWAGPDAVVVRLASGAARVIPLDGRPPFDLPDGAVPLAVAPAG"
BASE COUNT          522 a         1199 c         1184 g          453 t
ORIGIN      
        1 gtggtggttc gtgaccacga gcccgttcgg cgagacgaac gacgccgagc agcccggcac
       61 gcggagcacc gacaggcgcg cccgctcgag ccacgcggcg tcgggccgga agccgtaggt
      121 ggcctccagg tagtcgagcg gcggattctc gaagagccac atcttgccgt tgtccagcgg
      181 tccggactcg accggtacgg gcgcctcctg ggcccggacg gcggatgcgg acaggaagag
      241 ggcgagcacg agcgcggcgg acgcgcggaa tgcgggtgcg gacgacattg gtaaaccccg
      301 ttgcggtggg acgcgggcaa gatcaggcga aggggattct tttgcatgag gatgcgttga
      361 atcgcggttc tcacccacca ccgtccctcg ccgccgtgtc cgccgagact cccgaagccg
      421 cccgccccgc ggccgcaacg aacttcatcc ggcagatcat cgagaacgac ctgaagtcgg
      481 gctcgacggg cgggcgcgtc gtcacccggt ttccgcccga gcccaacggc tacccgcaca
      541 tcggccacgc gaaggccatc tgcctgaact tcgacatcgc ggcggactac ggcgggacgt
      601 gcaacctccg gttcgacgac acgaaccccg agaccgagga catggagtac gtccgcgcca
      661 tggagcgcga catccgctgg ctcgggtatg actggggcga gaaccggttc ttcgcgtcgg
      721 actacttcga ggagctctac gacctggccg tccgcctcat ccggggcggg aaggcctacg
      781 tcgactcgca gtccaccgac gagatccgcc tgacccgcgg aaccgtgacc gagccgggcc
      841 gcccgagccc gtaccgggac cggtccgtgg acgagaacct ggacctcttc cgccggatga
      901 aggagggcga gttccccgac ggcgcgcacg tgcttcgggc ccggatcgac atggcgtcgc
      961 ccaacatgct tatgcgcgat cccgtgctgt accgcatcaa gcacgcggag cactaccgcc
     1021 gcgggaacga ctggtgcatc tacccgctct acgatttcgc gcatccgctc tcggacgcca
     1081 tcgagggtat cacgcattcc ctgtgcacgc tcgagttcga cgtgcaccgg ccgctttacg
     1141 actggctggt cgatgccctc ttcgcaccgc cgcgcccgca ccagtacgag tcggcccggc
     1201 tgaacctgga ctacaccgtc atgagcaagc gcaagctgct gcagctcgtg aaggagggcc
     1261 acgtggccgg gtgggacgat ccgcgcatgc ccacgatctc cggcatgcgc cggcgcggct
     1321 acacgcccga gtcgatccgg cggttctgcg cgctgatcgg ggtggccaag gccgacaacc
     1381 gggtggacct gtcgctgctc gagtacgccg tccgggacga cctgaaccac cgggcgcccc
     1441 gcgtgatggt cgtcctcgat ccgctcgagg tagagctcac gaactggccc gccgaccgcc
     1501 tcgagtggct ggatgcgccg tactggccgc acgacgtgcc gaaggagggc tcgcgccagg
     1561 tgcctttcgg ccgcacgctc ctcatcgagc gggacgactt catggaggac ccgccgaagc
     1621 ggttccaccg cctgtccccc ggcgaagaag tccggctgcg gtacggctac atcatccggt
     1681 gcgacgaggt ggtgaaggac gcggccggaa gggtgcagcg gctgaggtgc tcggtcgatc
     1741 ccgacacgcg gagcggcgag acgggcggac gccgggtgaa gggcacgatc cactgggtgg
     1801 aggccacgca cgcggttccg gtcgaggtgc gcctgtacga ccgcctgttc accgtgccag
     1861 accccgagga cgttccggag ggagagtcct tcaccgaccg gctcaacccg cgctccggcg
     1921 atctggtgcc ggcggcgatg gccgagcact cgctccgcgc ggccggcccc ggcgaccgct
     1981 tccagttcga gcggcagggc tacttcttcg tggatcccga ggactcggcc gaagggcgac
     2041 cggtgttcaa ccggaccgtc acgctgcggg acacctgggc gaagatcgcc tcggacggag
     2101 gcggccggcc tgccgcgaag aagacgcgcg acgcggcccc gcccgctccg cccgccccgt
     2161 ccgcgccgcc cgttcccgcg atgacctcga agcaggaggc ccgcgcgcgc gagctcgccg
     2221 agcgctacgg catcgcgttc gaggacgcgg cgctcctggc cacggacccc gacctgcgcg
     2281 ccttcttcga ggcggcggcc ggccgctcca agcacccgcg cccgatcgcc aactgggtcg
     2341 tgcacgacct gcagcgtgag cggaaggagc tcccgctcgc cgagatcgcg ctcacgccgg
     2401 agcacctggc cgagctcgtg gacctggtcg aggacggcac gctgtcgagc cggctcgcga
     2461 aggaggtgtt cgctgacgtc ctctcgacgg gccgctcgcc ccgcgacgtc gtcgacgaac
     2521 ggggcctcgt gcagatcgac gaccgggacc acctcgccgc cgtcgcgcgg gaggtcgttg
     2581 ccgcgttccc ggaccgggcc gacgcctggc gcggcggcaa ggcggggctg atcggtttct
     2641 tcgtgggcca ggtgatgcag cgcaccggcg gaaaggccaa tccgaagctg acacgcgagc
     2701 tgctcgagga ggcgctggca ggctgagccc tgccctcacc ccgccggcgc gaccgccagg
     2761 ggtacggccc cgtccggaag gtcgaacggc ggcctgccgt cgagcgggat cacgcgcgcg
     2821 gcgccggacg ccaggcggac gaccacggca tccgggccgg cccacccggc gggctcggcc
     2881 ccctcgctgc catcgagcac gaccgcgtac ggatccgcga acgtcctcac ggccagaacc
     2941 gtggcgccgg ccgccgtctg gcggtacagg atgcgtgagc cgtccggcga ccagtgggga
     3001 tcgaactcgg acgggccgtc cgccgtcggc gcgtgcaggt acgtggatcg gcccgacgcc
     3061 acgtcgaaga tcgagacgag ccgctgcccc ggccgatccg ggttctggac gtcgtaggcc
     3121 agctccgtgc cgtccggccg ccaggcgaac cgctcgggtc ggtaccggaa tccgaccacc
     3181 ggtcgctcgt ccgagccgtc cggcccggca atcatgaacg tcgagtccgc gacgtactgc
     3241 cccgaggagc gttcgtacac gagctcgcga tgcacgtacg cgacgtgagc cccgtcgggc
     3301 gaggcggtca gatggtgcat gcgccgggcg tcgaccgtgg agcgcgtcgt gcagtccg
//
LOCUS       DBNHQZ010000338         4909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3078549, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000338 DBNHQZ010000000
VERSION     DBNHQZ010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3078549"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="AC16YU_09065"
     CDS             complement(<1..747)
                     /locus_tag="AC16YU_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR6065415.1"
                     /translation="MKRIASILLLAALLAPTAGAQHWLHDDGSRTNRSMFRPIEDFPD
                     PNQYRNGAGLPGPAYWQQQADYVIEARLDTMEHRIHGTERVTYHNNSPDVLRYLWVQL
                     DQNVRSIEHSRTYQTQNALPERISPMFRRFVGVDLFDGGHDVTRVQLVENGRLVDAEY
                     RINDTVMRIDLLRPLEPGAAQSFEIDWSFVVPDQARGAKERVRDGWLYVIAQWFPRMS
                     VYDDVNGWQTDQFIGRGEFYLNFGDYDVKLT"
     gene            complement(771..1373)
                     /locus_tag="AC16YU_09070"
     CDS             complement(771..1373)
                     /locus_tag="AC16YU_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6065416.1"
                     /translation="MLSSFLVYLRLGFEHIADWQAYDHLLFVAALCVSYPLRAWRPLL
                     VLVTAFTMGHSVTLVLATLNLVSVDPVLVETLIPVTIVLTSLVNVVESSRLSDILAEP
                     ERARRIKYGTALFFGLIHGMGFSSFLRAALGAEERITLPLFAFNVGLEVGQIVIVTGL
                     LLLGAAVVRFRLLDRRDWILVVSGAAGGIALTMILDRIAA"
     gene            complement(1394..1996)
                     /locus_tag="AC16YU_09075"
     CDS             complement(1394..1996)
                     /locus_tag="AC16YU_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065417.1"
                     /translation="MESAPDAMRPRVLPVLALLVVVCLPACRTAGTYSERGEHIELMK
                     GLLAHFADSEWYAYGTAYADSALIFVNSVSPMTVEERLASLQSQRSLFEDVSIADPLF
                     GEVHNADGEVWVLTWGSWTGRVRDSEWQVAVPVHIAARFMDGKVDQEWVYMDQAPLDV
                     AALEAAVLDAIPVSDSAAVAPDAGSAAPSDSSSAAPAGMP"
     gene            2272..2721
                     /locus_tag="AC16YU_09080"
     CDS             2272..2721
                     /locus_tag="AC16YU_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065418.1"
                     /translation="MKASKLLLGIAVVAYVVSLVLPTVNQGQGIIFAVWGWVAFAMTQ
                     AWGIVWLANPAVVASGWLLFADRSPALRRLLAVAALLLALFALTIDEHGGAHQGVALQ
                     VGYWVWLGSIVIVLAAVLLPAPHGEAAPPARTGPEPGLAGIDAVTGA"
     gene            3013..3945
                     /locus_tag="AC16YU_09085"
     CDS             3013..3945
                     /locus_tag="AC16YU_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR6065419.1"
                     /translation="MNARRPAASALPVVRRLLREQLERAVSCEARVGADGDPDAVHDF
                     RVAQRRTRVLLREYGDLLPTESDTLRKDLQWLARRTGPVRDLDMLLEGVRRRAAGAGV
                     RRRPGLATLALALAEKRRLAASRLERRLAAPRYGQLMRTWRSLAGRAGNGPGAAARAA
                     FAAATRRRLRDRYARLVATVRRLPEDAPDPAIHRARIAAKAVRYLLEFLDAVEPSGDV
                     TLAIGGLKALQDRLGSFQDDVVHAAMLEDRTSAGALAAAARPAAALRAARHEAARIAR
                     RRDRSRRSVVRALGRMSGPRFQSRLGRLTGPAEG"
     gene            4073..>4909
                     /locus_tag="AC16YU_09090"
     CDS             4073..>4909
                     /locus_tag="AC16YU_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HIR6065420.1"
                     /translation="MPRIVPALAGLVLIAGTGASAQVPAFSLNEHEYFERGGVNVMAF
                     QDIYPDGHQAGVSVIQHAVRLATNGDIRLETTPGQWSPMPRQLDRVVDRETGTITTTL
                     AYPDPSKDRTGFNPIEYPDVAFTYRVAVLAEGDAVRVRVDLDEPLPDEWIGRVGFNME
                     LFPGAYFGHAWYLGGTSGHFPMQPNGPSRTRALGSRKPVEIAEPVAMATGRMLSIAPG
                     SDDRRMTVESLTGDLSLYDGRIQHNNGWFVVRETVSVGAAAGAVEWILRPHAVEGWMY
                     EPV"
BASE COUNT          749 a         1705 c         1690 g          765 t
ORIGIN      
        1 cgtcagcttc acgtcgtagt cgccgaagtt caggtagaac tcgccccggc cgatgaactg
       61 gtcggtctgc cagccattca cgtcgtcgta gacgctcatc ctcgggaacc actgcgcgat
      121 cacgtagagc cacccgtcgc gcacccgctc cttcgctccc cgcgcctggt ctggcacgac
      181 gaagctccag tcgatctcga acgactgcgc ggcgcccggc tccaggggcc gcaggagatc
      241 gatccgcatc acggtgtcgt tgatgcggta ctcggcgtcg acgagccgtc cgttctccac
      301 gagctggacg cgcgtgacgt cgtggccccc gtcgaagaga tccaccccca cgaagcgccg
      361 gaacatcgga ctgatgcgct cgggcagagc attctgcgtc tggtaggtcc ggctgtgctc
      421 gatcgaccgg acgttctggt ccagctgcac ccacaggtag cgcagcacgt cgggcgagtt
      481 gttgtggtac gtgacgcgct cggtgccgtg gatccggtgc tccatggtgt cgagccgggc
      541 ctcgatgacg taatcggcct gctgctgcca gtacgcggga cccggaagcc cggcgccgtt
      601 gcggtactgg ttcggatcgg ggaaatcctc gatcggacgg aacatggacc ggttggtgcg
      661 cgacccgtcg tcgtgcagcc agtgctgcgc gccggccgtg ggcgccagga gcgcggcgag
      721 aaggagaatc gaggcgatgc gtttcatgac ggaaggtttt ggaagagaac ttacgcggcg
      781 atccggtcca ggatcatggt gagggcgatg ccgcccgccg cgcccgaaac gacgagaatc
      841 cagtcgcggc ggtccagcag gcggaaccgg accacggccg ctcccaggag aaggagcccc
      901 gtgacgatga cgatctgacc cacctccagg cccacgttga aggcgaagag cggcagcgtg
      961 atgcgctctt ccgccccgag ggcggcgcgg aggaagctcg agaatcccat gccgtggatg
     1021 agtccgaaga acagggccgt tccatacttg atacggcgtg cgcgctcggg ctccgccagg
     1081 atgtcggaca gcctcgacga ttcgaccacg ttcaccaggc tcgtgagcac gatggtcacg
     1141 ggaatcaggg tctcgacgag tacgggatcg acgcttacga gattcagcgt ggcgagcacg
     1201 agcgtcacgg agtgccccat cgtgaacgcc gtgacgagga ccaggagtgg cctccaggcc
     1261 cggagcgggt acgagacgca gagcgcggcc acgaacagca ggtggtcgta ggcctgccag
     1321 tccgcgatat gctcgaaacc gagccggagg tagacgagga agctcgacag catggattcc
     1381 cgtgcgacgc gtcctacggc atccctgccg gcgcggcgct cgacgagtcg gacggtgcgg
     1441 cgctccccgc gtccggcgcc acggccgccg aatcggaaac ggggatcgca tcgagcacgg
     1501 ccgcctccag cgcggccacg tcgagcggag cctggtccat gtagacccac tcctggtcca
     1561 ccttcccgtc catgaaccgg gccgcgatgt ggacgggaac ggccacctgc cattccgagt
     1621 cccgaacgcg gccggtccac gacccccacg tcagcaccca gacctcgccg tcggcattgt
     1681 gaacctctcc gaacagcgga tccgcgatgg acacgtcctc gaagagactg cgctgcgact
     1741 gcagggacgc gaggcgctcc tccacggtca tgggcgagac cgagttcacg aatatgaggg
     1801 ctgagtccgc atacgcggtg ccgtacgcgt accactcgga atccgcgaaa tgggcgagca
     1861 ggcccttcat gagctcgatg tgctcgcccc gttccgagta ggtgcccgcg gtccggcagg
     1921 ccgggaggca gaccaccacg agcagggcga gaaccggcag gactcgaggt cgcatggcgt
     1981 cgggtgcgga ttccatggat gatcccccgc caaacggtcc atcggcggac gggatcctgc
     2041 ggcacaggat acgaccgccg ccgccgggtt tctcccggtc cccggcgcag gcatcggccg
     2101 attctcgtca tttcctttcc cggtgcgccg atgccgcaca cggcttgcgg gtgatgtccc
     2161 tacacggaca cccaaatctg ctttctttct gtcgcggaac cgcttcgggg cttgcgcccg
     2221 gcgcccgttt ccttgttatt ggcatccggt tccacacgca aactcgctcg catgaaagcc
     2281 tcgaagctgc tgctcggaat cgcggtggtt gcctacgtgg tgtccctcgt gctcccgacc
     2341 gtcaaccagg gacagggtat catcttcgcc gtgtggggct gggtggcctt cgcgatgacc
     2401 caggcctggg gaatcgtctg gctggccaat cccgccgtcg tcgccagcgg gtggctgctc
     2461 ttcgcggacc ggtcgcccgc cctgcgccgg ctgctcgccg tcgccgccct gctcctcgcc
     2521 ctcttcgctc tcaccatcga cgagcacggc ggcgcgcacc agggggtcgc gctgcaggtg
     2581 gggtactggg tatggctcgg gtcgatcgtg atcgtgctcg ccgcggtgct tctgcccgcc
     2641 ccgcacggcg aagcggcacc gcccgcccgc accggtcctg agcccggcct ggccggcatc
     2701 gacgccgtca cgggggcata gggcgtgaac gcgccctgga tttgcatttt tccgcgggat
     2761 ttacgagaaa tgggtatgcc gtgcagcccg cggcatgtat ttacagtcct acgtacagcg
     2821 ggtgaccagg cctatgccag gtaaatcgaa ttcgatcggt ccggcgcgtg ccccgatgcc
     2881 ggagaagagc tccaccaggg accagttcgg atggtggttc cgctggtgcc actccgacga
     2941 tcccgtatcc tactggaaag agcggcagga gaagcgccgc tgaccgttcc cggggcgcat
     3001 cccggagcgt ccatgaatgc caggcgtcca gcggcttcgg cccttcccgt ggttcggcgc
     3061 ctgctccggg agcagctgga gcgtgccgtg tcgtgcgagg cgcgcgtggg ggcggacggc
     3121 gatccggacg cggtgcatga cttccgggtc gcccagcgcc gcacccgggt cctgcttcgc
     3181 gaatacgggg acctgctgcc cacggagtcc gacacgctcc ggaaggacct gcagtggctc
     3241 gcccgccgca cgggtcccgt gcgggacctg gacatgctgc tcgagggagt ccgccgccgc
     3301 gccgcgggcg caggcgtgcg gcgtcgcccg ggcctcgcga cgctggccct cgccctcgcc
     3361 gagaagcgcc ggctggccgc gtcgcgcctc gagcggcgac tcgccgcgcc gcggtacggg
     3421 cagctgatgc ggacctggag gagcctcgcg ggcagggccg ggaacggccc gggggccgcc
     3481 gcgcgcgcag cctttgccgc cgcgacgcgc cgacggcttc gggaccgcta cgcgcggctc
     3541 gtcgcgaccg tccgacgact cccggaagat gcgcccgacc cggccatcca ccgcgcccgc
     3601 atcgcggcca aggcggtccg gtacctgctc gagttcctgg atgcggtcga gccgtcggga
     3661 gacgtgaccc tggcgatcgg cggcctcaag gcccttcagg accggctcgg ctcgttccag
     3721 gatgacgtgg tgcacgccgc gatgctggag gaccgtacct cggccggggc gcttgccgcg
     3781 gccgcacgtc ccgcggccgc gctgcgcgcc gcgagacacg aggcggcccg gatcgcgcgc
     3841 cggcgggacc ggagccgccg ctccgtggtc cgtgcgctcg ggcgcatgtc cggaccccgc
     3901 ttccagtcgc gcctcggccg cctgaccggt ccggccgagg gctgaggcgc ccggggcgac
     3961 cgcatttcga cgacgattct cgtgcgcccc tcggcacgcg tggcatccgg cgactcggaa
     4021 ccggatcttg accggcagta ccgaaccttc cgccccgagg cggcttcgag ccatgccccg
     4081 catcgtaccg gcgctcgccg gtctcgtcct catcgccggc accggagctt cggcccaggt
     4141 gcccgccttc agcctcaacg agcacgagta tttcgagcga gggggcgtga acgtgatggc
     4201 cttccaggac atctatccgg acggccacca ggcgggggtc tcggtcatcc agcacgccgt
     4261 ccgcctggcg accaacggcg acatccggct ggagacgacg cccggtcagt ggtcccccat
     4321 gccgcgccag ctggaccggg tggtggaccg ggagaccggc acgatcacga cgacgctcgc
     4381 ctaccccgat ccctcgaagg accggaccgg cttcaatccg atcgagtatc cggacgtcgc
     4441 gttcacgtac cgcgtcgccg tgctggcgga gggcgatgcc gtgcgcgtcc gcgtcgatct
     4501 cgacgagccc ctgcccgacg agtggatcgg ccgcgtgggt ttcaacatgg agctgttccc
     4561 cggggcgtat ttcggccatg cgtggtacct cggaggcacg tcggggcatt tcccgatgca
     4621 gccgaacggg ccctcgcgca cgcgagccct cggtagccgt aagcccgtcg agatcgccga
     4681 gcccgtcgcg atggctaccg gccggatgct gagcatcgct cccggctcgg acgaccgccg
     4741 catgacggtc gagagcctta cgggagacct ctcgctgtac gacgggcgca ttcagcacaa
     4801 caacgggtgg ttcgtggtcc gcgagacggt ctccgtgggc gccgcagccg gcgcggtcga
     4861 atggatcctc cggccgcacg ccgtggaggg gtggatgtac gagcccgtc
//
LOCUS       DBNHQZ010000339         3311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2404316, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000339 DBNHQZ010000000
VERSION     DBNHQZ010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2404316"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="AC16YU_09095"
     CDS             complement(<1..1659)
                     /locus_tag="AC16YU_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR6065421.1"
                     /translation="MQESPRNPSAPVVDTPRSAVDDPAALYRALMLPRLIEDKMLRLI
                     RQGRLAKWFSGYGQEAIAVGCAWALEEDDIILPMHRNLGVWTTRGVPLEPLFCQLMGR
                     SGGFTRGRDRTFHFGLPEHGIIGMISQLAAMLPVACGFGLAARLDGSQRVAVAFIGEG
                     ATREGDFHEALSLAAVWRLPVLFVVENNGWGLSTPTEEALPIVHIADAGPGYGIPSRI
                     IEGNELETVIREVRDAADHARAGNGPVLLEMKTYRMRGHEEASGNKYVPKDQLESWAR
                     KDPVLQFEQALGDRLGDEDRESIRRELRQRVEEVAEWAIRQPEVEGTPDDEEADLYRA
                     APAVHVVDSGARTEKRFIDAVSDGLAQALEEDRRVLLLGQDIAGYGGVFKVTAGFVDR
                     FGPERIRNTPIIESGVVGAALGLALSGYRPIVEMQYADFISCGFNQIVNNLATTHYRW
                     GASVPVTIRAPFGGHIGAGPFHSQSMEGWFCHVAGLKVVSPSTPADAKGLLLASIDDP
                     NPVLFFEHKALYRSVKGLVPDGPVRVPIGRAAVAREGTDATIVSW"
     gene            complement(1676..3073)
                     /locus_tag="AC16YU_09100"
     CDS             complement(1676..3073)
                     /locus_tag="AC16YU_09100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6065422.1"
                     /translation="MSRRTELAVLLAVDALALAGANLLWHSARFRWGWLSDPVFSPEP
                     AVLGTVVAILTLYWLVTFLFFGMYRERYAASRFDEIVLLVKVVTVGILVLFFLLFIES
                     LDAYSARVNLLFYWLAVFVLVAAGRIVVRSVQKALILRGRGLHRALVVGWDDRLEPLY
                     EDVARYPEAGLQIVGALRLGRGPGEPGAGENTSRSMAELPRLIDELDVQDVLIALGPD
                     AHDQLLEVLRLCDGKSVSLKLVPDFYSIIGGMARTEHMYGLPLIEVLPEPMPAWEQST
                     KRIIDLVVSTLVLAVGMPAWIVLALLIRITSPGPAIYRQQRVGRHGREFTMYKFRTMR
                     QDAEAQTGPVWAVADDPRYTPVGRWLRRTRLDEIPQFWNVFKGDMSLVGPRPERPFFV
                     EKLAREIPLYNRRHRVKPGITGWAQVKWKYDGSLDDVRQKVKYDLFYIENMSLRRDFQ
                     IIARTITTALRAIGH"
BASE COUNT          534 a         1172 c         1105 g          500 t
ORIGIN      
        1 ccacgagacg atggtggcat ccgtcccttc cctggccacc gccgccctgc cgatcggcac
       61 gcggaccgga ccgtcgggca cgagaccctt caccgagcgg tacagcgcct tgtgctcgaa
      121 gaagagcacc gggttcggat cgtcgatcga ggcgagcagc agccccttcg cgtcggcggg
      181 cgtcgagggg ctgacgacct tgaggcccgc cacgtggcag aaccacccct ccatcgactg
      241 cgaatggaag ggacccgcgc cgatgtgacc gccgaaaggc gcgcggatcg tcaccggaac
      301 ggaagcgccc caccggtaat gcgtggtcgc caggttgttc acgatctggt tgaatccgca
      361 cgagatgaag tccgcgtact gcatctcgac gatcggacgg tacccggaga gcgccaggcc
      421 cagggccgcc ccgacgacgc cgctctcgat gatgggcgtg ttccggatcc gctccggtcc
      481 gaagcggtcc acgaatcccg ccgtcacctt gaacacgcca ccgtaccctg cgatgtcctg
      541 cccgaggagc agcacgcgcc ggtcctcctc cagggcctgc gcaagcccgt cgctcacggc
      601 gtcaatgaat cgcttctccg tccgggcccc ggaatccacg acatgcacgg cgggggcggc
      661 acggtacaga tcggcctcct cgtcgtcggg cgttccctcc acctcgggct gccggatggc
      721 ccactccgcg acctcctcga cccgttggcg aagctcgcgg cggatcgact cccggtcctc
      781 gtctccgagc cggtcaccga gggcctgctc gaactggagg accgggtcct tgcgcgccca
      841 cgactcgagc tggtccttcg gcacgtactt gttgccggac gcctcctcgt gcccccgcat
      901 ccggtaggtc ttcatctcga ggagcacggg gccgttgccg gcgcgcgcgt ggtcggcggc
      961 gtcgcgtacc tcccggatca ccgtctccag ttcgtttccc tcgatgatcc gggagggaat
     1021 gccgtagccc gggccggcgt ccgcgatgtg cacgatcggc agggcctcct cggtgggcgt
     1081 cgacaggccc caaccgttgt tttctaccac gaagagcacg gggaggcgcc acacggcggc
     1141 caggctcagg gcctcgtgaa aatccccttc ccgcgtcgcg ccctccccga tgaaggcaac
     1201 ggcgacacgc tggctcccgt cgagccgggc ggcgagcccg aagccgcacg cgaccggcag
     1261 catggcggcg agctgcgaga tcattccgat gatgccgtgc tcgggaagcc cgaaatggaa
     1321 cgtgcggtcg cggccccggg tgaatccgcc gctccgcccc attaactggc agaagagcgg
     1381 ctcgagcggc accccccggg tagtccagac acccaggttg cggtgcatcg gcaggatgat
     1441 gtcgtcctcc tcgagagccc acgcgcatcc gaccgcgatg gcctcctgcc cgtacccgct
     1501 gaaccacttg gcgaggcgcc cctggcggat gaggcggagc atcttgtcct cgatcagccg
     1561 gggaagcatg agcgcgcggt acagggccgc cggatcgtcc acggcgcttc ggggggtgtc
     1621 cacgaccggc gcggagggat tcctgggtga ttcctgcatg gcgggggcag ccgtcctagt
     1681 ggccaatcgc cctcagggcg gtggtgatgg tccgggcgat gatctggaaa tcccgtcgca
     1741 ggctcatgtt ctcgatgtag aacagatcgt atttcacctt ctgccggacg tcgtccaggc
     1801 ttccgtcgta cttccacttc acctgggccc agcccgtgat gccgggcttg acgcggtgcc
     1861 gccggttgta gaggggtatc tcccgggcca gcttctcgac gaagaacggg cgctccgggc
     1921 gtggccccac gaggctcatg tctcccttga acacgttcca gaactgcggg atttcgtcca
     1981 ggcgggtccg ccggagccac cgtccgacgg gcgtgtagcg cggatcgtcc gcgaccgccc
     2041 acacgggtcc ggtctgcgcc tccgcgtcct gcctcatcgt gcggaacttg tacatcgtga
     2101 actcgcggcc gtgccggccg acccgctgct gccggtagat ggccggcccc ggggacgtga
     2161 tccgtatgag gagcgcgagc acgatccagg cgggcatgcc cacggcgagc acgagcgtcg
     2221 aaaccaccag gtcgatgatg cgcttcgtgc tctgctccca ggccggcatc ggctcgggca
     2281 ggacttcgat gagcgggagc ccgtacatgt gctcggtccg ggccattcca ccgatgatcg
     2341 aatagaagtc cggaaccagt ttgagcgaca ccgacttgcc gtcgcacagc cgcagcacct
     2401 cgagcagctg gtcatgggcg tcgggcccga gggcgatgag cacgtcctgc acgtcgagct
     2461 catcgatgag ccggggcagc tcggccatcg aacgggaggt gttctcgccc gctccaggct
     2521 ccccggggcc gcgtccgagc cgcagcgccc ccacgatctg gaggcccgcc tcggggtacc
     2581 gggcgacgtc ctcgtagagc ggctccagcc ggtcgtccca gcccacgacc agggcgcggt
     2641 gcagccccct gccccgcagg atcagggcct tctgcacgga ccggaccacg atccggccgg
     2701 ccgcgacgag aacgaacacg gcgagccagt agaagagcag gttcacccgg gcggaatagg
     2761 cgtccagact ctcgatgaag agcaggaaga agagcaccag gatgccgacc gtgacgacct
     2821 tcacgaggag cacgatctcg tcgaaccgcg aggccgcgta ccgctcccgg tacatgccga
     2881 aaaacaggaa cgtcacgagc cagtagaggg tcaggatcgc caccaccgtc ccgagaacgg
     2941 ccggttccgg ggagaagacg gggtcgctca gccagcccca ccggaaccgg gcgctgtgcc
     3001 agagcaggtt cgctccggcc agcgcgaggg catcgacggc gagaagtacg gcgagttcgg
     3061 ttcggcggga cacgggggcg ggatgttcgg ccgggctcag cggcgcccga cgacccggag
     3121 tccagtaagg tacccgatgc cctttctacc gtgcaacagg cggccgcccg gtcgcttgtg
     3181 aggccgcatg cgcataactt ccccgcgccc atcggccgtc cggccacgcc atgccccgac
     3241 tacggattcc gcctctcgcg ctggccctcc tcgccgtcct ggccgagccg tcgggcgcgg
     3301 ccgcccagac g
//
LOCUS       DBNHQZ010000340         5814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_642076, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000340 DBNHQZ010000000
VERSION     DBNHQZ010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_642076"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="AC16YU_09105"
     CDS             <1..758
                     /locus_tag="AC16YU_09105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6065423.1"
                     /translation="VDALVADVHEVGADVVAVSGDLTQRALPRQYAAARSFLARFGPP
                     VLVVPGNHDVYAWWRPAGRLMDPLRRYRHYVTDDLTPSLRVGDVSMFGLNSATGWTVK
                     GGHVRPPDRRRMTEFFRSAPPDDFRVLVIHHHLTRVRALGPHDVARNADETLDAAVRC
                     GVDLILCGHLHISHIEPIEIVPAEHRLVIASAGTATSSRGRRKHRRSNFYNVVTIRPD
                     SFLIEERLFDPGTGTFRTEFETSFERRPAPAEA"
     gene            891..2633
                     /locus_tag="AC16YU_09110"
     CDS             891..2633
                     /locus_tag="AC16YU_09110"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6065424.1"
                     /translation="MRRIRYALFLLVLSVPVSRSAAAQSGTSTSANRSPVAVDVVRVL
                     SEDTRASIALPATDPDGDGITVEVVTAPRNGTVTVTGSDVTYVPRRDFSGADRFTYRV
                     SDGSSVSGSATVRLAVTPVNDPPAVTLEPGPARIREGEAYEFRFRAADPEGDAVEWRL
                     VDGPEGATLDPRSGRLRWTPEFDQAGVHRVRVGASDGQAESLSVATLTVLDTEVHSGT
                     FASIHLTSPIISPATGTASVTYDDVAQTLVIDATFSGLSSDRAAAAAYLGPFGEDQDK
                     LVDLVLQDDASNPGAASGRILFPGAFNPSATFQLGLLPFLFPDGSVTAQMVVDSLRSG
                     KVYIVVTSTDHPEGEIRAHLRSDAANSAPEAAVLDAPTTVTIEGDPQATGLEVTWSAA
                     VDPDGDPTRQVFGLWTDASLEDMHLMVVDEGSHAVSFTVDGLAGLFSELTGGSTGSTT
                     VYLAVLTTDGAHYTVSNTVAVSLVRGQVTATDDGGVVPDVFMLRGNFPNPFNPTTTVQ
                     FDLPAPAEVEVYVTDLLGRVAISVPSQQISAGLRRQIPIDASSLASGIYIYRVTARTA
                     ETTHVATGTMTLLK"
     gene            2734..4194
                     /locus_tag="AC16YU_09115"
     CDS             2734..4194
                     /locus_tag="AC16YU_09115"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR6065425.1"
                     /translation="MSRIPLPVSGAYSAGALRRLVLTAALVVVPLQGALSQQPLQMDL
                     DHSSFAFDDASSLVEMYLAFEASTLRFTADSTGFAAELPIDVSVFRASDAVLEGTPGD
                     AVWADTLDLVFVLADTSGIRPGQQFVHQVRATVPPGEYEIVVDVPEDASVGRQSLSLR
                     REILVPDFSDPGVVGLSDVTLASEIRRALGSNEAFYKSGLVVRPNANQLFGLGLPHVY
                     YYTEAYNLEALAPAETGYTVMAYISEVNLPQPLPDLQRRVTRPLRSPDVLVGSFDVSG
                     LPSGSYFLRIALLNSGNESLVERSRRFFVYNPGVERAPVAGVEEDFETSQYATMTAEQ
                     VEQAGRHIDIIASTTERRRLDGIQDLDERRRFLMEFWRKRDPVPSTAVNEFQEEFYQR
                     LQYANDRYTSTFTEGWETDRGRTIIKYGPPSRVDPHLYDRGYAPYEVWEYNNIPGEGQ
                     AVFVFADQGNFGLFELIHSTVTGERRLANWMEELQR"
     gene            4194..5699
                     /locus_tag="AC16YU_09120"
     CDS             4194..5699
                     /locus_tag="AC16YU_09120"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR6065426.1"
                     /translation="MAMDFVYIRDLAAHAGREVTVKGWLYNKRESKGLAFLVVRDGTG
                     LVQCVVPEDAVAGDSWAAASGATQESAVAVTGTVRHDDRQVGGHEIQASAVEMISLAE
                     EYPITPKEHGIEFLMNNRHLWLRSRRQWAVMRIRNRIIYSMHQFFQEQGFVQMDAPIL
                     TGNAVEGTSTLFTIDYFGRPAYLTQSGQLHGEAMAMAMGKIYTFGPTFRAEKSKTRRH
                     LTEFWMIEPEMAFYDLDMNMELAEALLVRLVGDVLSGFEEELAILERDPEPLRRAVGT
                     FPRITYDEAVDILRSDETAAMMDQRIAGLEEEAAALGAELEAGRKAYGQAKKAEKRRF
                     DAREIRINQRLADIEEEQRYLPGWKVSARNFEWGNDFGGSDETVITWHFDRPLIVHRF
                     PHHFKAFYMKRDPADDRLALGMDVLAPEGYGEIVGGGERATDLAFLREQVRVHGLPEE
                     AFDWYFDLRRFGSVPHSGFGIGLERTVAWICGLKHLREAIPFPRLIDRIHP"
BASE COUNT          885 a         1995 c         1986 g          948 t
ORIGIN      
        1 tggtggacgc tctcgtcgcc gacgtgcacg aggtcggtgc ggatgtcgtg gccgtgagcg
       61 gagacctgac gcagcgcgcg ctccccaggc agtatgccgc ggcccgcagc ttcctggccc
      121 ggttcggccc gccggtgctc gtggtgccgg gcaaccacga cgtgtacgcg tggtggcgcc
      181 ccgcgggccg gctgatggac ccgctgcgcc ggtatcggca ttacgtcacg gacgacctca
      241 cgccctcgct ccgggtgggc gatgtctcga tgttcgggct gaattccgcg acggggtgga
      301 ccgtgaaggg tggtcacgtg cgcccgcccg accgccgtcg gatgaccgag ttcttccgtt
      361 ccgcgccgcc tgacgatttc cgggtgctgg tgatccacca tcacctgacg cgcgtgcgcg
      421 cgctcggacc gcacgacgtg gcccgaaacg cggacgagac gctcgacgcg gccgtgcggt
      481 gcggcgtgga cctgatcctg tgcggccacc tgcacatctc gcatatcgag ccgatcgaaa
      541 tcgttccggc ggagcaccgg ctcgtcatcg cgagcgcggg cacggccacg agcagccggg
      601 gccggcgcaa gcaccgccgg tccaatttct acaacgtggt gacgatccgt ccggactcgt
      661 tcctgatcga ggagcgtctc ttcgacccgg gtacgggcac cttccgtacg gaattcgaga
      721 cttcgttcga gaggcggccc gcccccgcgg aggcctgaca gggctcgccg ggcggtcagt
      781 gccgctccgg ggctcgcgca cggccccggg atccggaggc gaaatatctg gaatcgctcc
      841 atttggcggc cgcatgtctt atcttgccgt tcgcacccgg gcgaaacggc atgaggcgaa
      901 tccgctacgc actttttctg ctggtccttt ccgttccggt ctcccgcagc gccgcggcgc
      961 agtccggcac gtcgacgagc gcgaaccggt cgcccgtcgc ggtggacgtc gtgcgggttc
     1021 tttcggagga cacgcgcgcg tcgatcgcgc ttccggccac cgacccggac ggggacggca
     1081 tcaccgtcga ggtcgtcacc gcgccccgca acgggacggt cacggtcacg ggttcggacg
     1141 tcacgtacgt gccacggcgg gatttctcgg gcgcggaccg gtttacgtat cgagtcagcg
     1201 acgggagctc ggtctcgggc tcggctaccg tgcgcctcgc cgttacgccc gtcaacgatc
     1261 cgccggccgt gacgttggag ccgggcccgg cccggatccg ggagggcgag gcgtacgagt
     1321 tccgcttccg cgccgcggac cccgaaggcg atgcggtgga atggcgtctg gtcgacgggc
     1381 ccgaaggggc cacgctcgac ccgcgttccg gcaggctgcg ctggacgccg gagttcgacc
     1441 aggccggcgt gcaccgcgtc cgcgtgggag cgtccgacgg gcaggccgag tcgctttccg
     1501 tggcgaccct caccgtgctg gacacggagg tccactcggg cacgttcgca tcgatccacc
     1561 tgacctcgcc gatcatctcg ccggccaccg ggacggcatc ggtcacgtac gacgacgtcg
     1621 cacagaccct ggtgatcgac gccacgttct ccgggctctc gtccgaccgg gcggcggccg
     1681 cggcgtacct gggcccgttc ggggaggacc aggacaagct cgtcgatctc gtgctccagg
     1741 acgacgcttc gaatcccgga gccgcgagcg ggaggatcct cttcccgggc gcgttcaacc
     1801 cgtcggccac gttccagctg ggtctgcttc ccttcctgtt tccggacggc agcgtgacgg
     1861 cccagatggt cgtcgactcg ctgcgttcgg gcaaggtcta catcgtggtg acgtcgaccg
     1921 accacccgga gggcgagatc cgtgcccacc tgcgctcgga tgccgcgaac tcggcgccag
     1981 aagccgcggt gctcgacgcc ccgacgaccg tgacgatcga gggcgacccc caggcgacgg
     2041 ggttggaggt gacctggtcc gccgcggtcg atccggacgg cgatcccacg cgccaggtgt
     2101 tcggcctctg gaccgacgcc tcgctcgagg acatgcacct catggtggtc gacgagggct
     2161 cgcacgcggt gtcgttcacg gtggacgggc tcgcggggct cttctccgag ttgaccgggg
     2221 gatccacggg ttcgaccacg gtgtacctgg ccgtccttac caccgacggg gctcactaca
     2281 cggtttcgaa cacggtcgcg gtgagcctcg tgcgcggcca ggtcacggcg acggacgacg
     2341 gcggagtggt gcccgacgtg ttcatgctcc ggggcaactt ccccaatccg ttcaatccga
     2401 cgaccacggt acagttcgac ctgcccgcgc cggccgaggt cgaggtctac gtgacggacc
     2461 tgctcggccg cgtcgccatc tcggtgccgt cgcagcagat ttcggccgga ttgcgccgcc
     2521 agatcccgat cgacgcatcg tcccttgcgt ccggcatcta catttaccgg gtaacggccc
     2581 gtacggccga gaccactcac gtggccacgg ggacgatgac cctgctgaaa tgacggttcg
     2641 gaaggtcggg gaccccgttt gatcccggag gcgtatatcg ttgcctgttg cgcccccacg
     2701 agcgttccct cacctatcac agaccgtttc ggcatgtcgc ggatcccgct tcccgtctcc
     2761 ggcgcgtaca gtgcgggcgc cctacggcgg ctcgtgctga cggcggcgct cgtggtcgtt
     2821 cccctgcagg gagcgctttc gcagcagccg ctccagatgg acctggacca ttcgtcgttc
     2881 gccttcgacg atgcgtcttc gctggtggag atgtacctcg cattcgaggc atccaccctg
     2941 cgattcacgg cggacagcac gggcttcgcg gcggagctgc cgatcgacgt ctccgtgttc
     3001 cgggcgtcgg acgccgtgct cgaggggacc cccggggacg cggtgtgggc cgatacgctc
     3061 gacctcgtgt tcgtgctcgc ggacacgtcc ggcatccggc cgggccagca gttcgtgcac
     3121 caggtccgcg ccaccgttcc gccgggcgag tacgagatcg tcgtggacgt gcccgaggac
     3181 gcatccgtcg gccggcagtc gctctcgctc cggcgcgaga tcctcgtgcc cgatttttcg
     3241 gatccgggcg tggtcgggct ctcggacgtg accctcgcgt ccgagatacg tcgggcgctg
     3301 ggcagtaacg aggccttcta caagagcggg ctcgtcgtgc gtccgaacgc caaccagctg
     3361 ttcggcctgg gactcccgca cgtctactac tacacggaag cctacaatct cgaggcgctc
     3421 gctcccgcgg agaccggcta caccgtcatg gcctacattt ccgaggtcaa cctcccgcag
     3481 ccgctgcccg atctccagcg ccgcgtgacc cggccgctac gctcgccgga cgtcctcgtc
     3541 ggctccttcg acgtgagcgg cctcccgagc ggctcgtact tcctgcggat cgccctcctg
     3601 aactccggga acgagtcgct cgtcgagcgg tcccgccggt tcttcgtcta caatccgggc
     3661 gtggagcggg cccccgtggc cggggtggaa gaggacttcg agacgagcca gtacgccacc
     3721 atgacggccg agcaggtcga gcaggccggc cggcacatcg acatcatcgc ctcgacgacc
     3781 gagcggcgcc gcctggacgg catccaggac ctggacgagc gccgccgctt cctcatggag
     3841 ttctggagga agcgggaccc cgtcccgtcg acggccgtga acgagttcca ggaggagttc
     3901 taccagcggc tgcagtacgc caacgaccgg tatacgtcga ccttcaccga ggggtgggag
     3961 accgaccggg gacgcacgat catcaagtac ggtccaccgt cgcgcgtcga cccccacctc
     4021 tacgaccggg gatacgcacc gtacgaggtc tgggagtaca acaacattcc cggcgaagga
     4081 caggccgttt tcgtgttcgc ggaccagggc aatttcggcc tgttcgaact catccactcg
     4141 accgtcacgg gtgagcggag actggcgaac tggatggagg agctgcagcg ctgatggcga
     4201 tggatttcgt ctacatccgg gacctcgcag cccacgccgg tcgcgaggtc accgtgaagg
     4261 ggtggctgta caacaagcgg gaatcgaagg gactggcctt cctggtcgtg cgtgacggga
     4321 ccggcctggt ccagtgcgtg gtgcccgagg atgccgtcgc gggcgattcg tgggcggcgg
     4381 cgtccggggc gacccaggag agcgcggtcg ccgtcacggg cacggtccgg catgacgatc
     4441 gccaggtcgg gggccacgag atccaggcgt cggccgtcga gatgatcagc ctggccgagg
     4501 agtaccccat cacgcccaag gaacacggca tcgagttcct gatgaacaac cggcacctgt
     4561 ggctgcggag ccgccgccag tgggccgtga tgcgcatccg gaaccgcatc atctactcga
     4621 tgcaccagtt cttccaggag cagggcttcg tccagatgga cgcgcctatc cttacgggga
     4681 acgcggtcga gggcacgtcc acgctcttca cgatcgacta cttcggccgt ccggcctacc
     4741 tgacccagtc ggggcagctg cacggcgagg ccatggcgat ggccatgggc aagatctaca
     4801 cgttcggccc cacgttccgg gccgagaagt ccaagacccg ccggcacctg acggaattct
     4861 ggatgatcga gcccgagatg gcgttctacg acctggacat gaacatggag ctcgccgagg
     4921 cgctcctcgt gcggctggtg ggcgacgtgc tgtccgggtt cgaggaggag ctcgcgatcc
     4981 tggagcggga tccggagcct ctgcgccggg cggtcggcac cttcccgcgc atcacgtacg
     5041 acgaggccgt cgacatactg cgcagcgacg agaccgcagc catgatggac cagcggatcg
     5101 ccggcctcga ggaggaggcg gccgccctcg gcgccgagct cgaggcgggg cggaaggcat
     5161 acggccaggc gaagaaggcc gagaagcgcc gcttcgacgc ccgggagatc cgcatcaacc
     5221 agcgcctggc cgacatcgag gaggagcagc ggtacctgcc cggctggaag gtgtccgccc
     5281 gcaatttcga gtggggcaac gacttcgggg gaagcgacga aacggtcatc acctggcact
     5341 tcgaccggcc gctgatcgtg caccgcttcc cgcaccactt caaggccttc tacatgaagc
     5401 gcgatccggc cgacgaccgg ctggcgctcg gcatggacgt gctggcgccc gagggctacg
     5461 gcgagatcgt cggcggcggc gagcgggcca cggacctcgc cttcctacgc gagcaggtgc
     5521 gcgttcacgg cctgcctgag gaggccttcg actggtactt cgacctcagg cggttcgggt
     5581 cggtgccgca ctcgggcttc gggatcgggc tcgagcggac cgtcgcctgg atctgcggac
     5641 tgaagcacct gcgggaggcg atcccgttcc cccgcctcat cgaccgcatc catccctgag
     5701 ccgggcgacg gccagcggcc gtcggcccct atccatccaa ccggagacag gatcatgaga
     5761 accagaacgt gggcctccgt ggtcatcctg gccgcgatgc tggccgttca gccc
//
LOCUS       DBNHQZ010000341         7688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1727425, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000341 DBNHQZ010000000
VERSION     DBNHQZ010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1727425"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="AC16YU_09125"
     CDS             <1..763
                     /locus_tag="AC16YU_09125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6065427.1"
                     /translation="QIARLLAFSLVMVTLAARIAVQIRFVEKGGRARLRARRQTEACR
                     LLVRILGVRVRTVGDPRPGVPRLVVCNHSGILDGFVLASAMPLATVAKAEMAGWPVMG
                     WVCRMVGMIFVDRSRRSDAARLVETVQERMRSGVPVLVFPEGTVGDGRSLLPFRSGAF
                     EAVADASDMEVLPVFLTATRVDAASGRDAHSALIWHHGESLMAHARRLLGAGRIDMEV
                     RLGEPISTAGRDRRNLAEASRRGVARLGETVLDPT"
     gene            883..2466
                     /locus_tag="AC16YU_09130"
     CDS             883..2466
                     /locus_tag="AC16YU_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR6065428.1"
                     /translation="MARLTGRNTAPLLYASLVVIGLLLGVLATVLVVRPASDTSGDRP
                     VVEVVQLTSRVAPLPYESDSLGANGYLDALSLNEAFKAVSSRVTPSVVFIEVESSGGG
                     FFHQFEGGGGFRLSAGSGVIVSDAGYVVTNHHVIEEATAIRVTLSDKREFDAELVGDD
                     PNTDLAVIRLVGADALPSIVLGDARSVEVGEWVIAVGNPFRLTSTVTAGIVSAIGRQV
                     NIINNDLAIENFIQTDAAINPGNSGGALVNLRGELIGIATAIATESGSYEGYGFAVPV
                     DLMQRVISDLVEYGEVRRGYLGVTIEPVNASLADDLGMDRVRGVFLRDVRPGMASWDA
                     GMRSGDVLVAIDGRPVDEPNALQGAIALYRPGDRVRAIVWRDGREIEMDVELFGREDS
                     RASEWFAELDGRSADPGLEPEEDDAPAPGDGSPHGNVQTLDEWGLGVIELSSRLSRAF
                     DVDGGVYIMYVEKDSPADAAGVPRNAVIVSVDGSPLTAIEKLKSALGAAAAAGSAVVL
                     EVVRRDGTVAFFEVEGLGG"
     gene            2610..5045
                     /locus_tag="AC16YU_09135"
     CDS             2610..5045
                     /locus_tag="AC16YU_09135"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR6065429.1"
                     /translation="MRARYSQPVPISVVLRDVMESWKRRPDAAPGSARESGGPPSTSP
                     RRGATEPASPVLPAGAVAPAPGCALPFAGIALVILLLVAAVPGAAHGQTVYVETFDAV
                     VAPDLPGGWTAGAGWATSTSSASTGSGLNNLAHTGMSVSEATLPPVDLSGVSSATLSF
                     LARRTSSYPLEGLRVTASLDGGASWPVEIMPTGAALPGAASAWEAVEASVPIALIGQS
                     GVLVRFEVSGGTTSGANLRLDDIRIAAGGGGSTGGGSSLGFSSGSSSALSPATDFTVA
                     LRLDYDATVGLGALQLRVTWDSDLLTWSGILPGAAVSDPAQWTVSAEPGLSSAMILVV
                     SHAAASLVAGTYDPIVELRFDVGPLSGGVPEDVTLALSDVIGAQAVPTGDDAGIGTST
                     STHLVTVTQAAPVFEAPASVDLGIAAMGQTISGTITVTNAAGGSDLVIGSVASDHPSF
                     GVSPSSATVPAGASFDFMLTYTPDGSVLGAESAQVSFAHDGSNGPVSVVSVLARAGAG
                     RGDANGDAMVDLLDFLIGIDVALGRILPSPGVVSALDLYPFASGDGSVDVRDLTVLAH
                     AIVQDTWPDGEPLPDPGAGGTAGKVGGSAGRANAIVALAETSSGLEVRVSTRVPLRGL
                     QFDLLVPASVPEWEVGDAAAVAPLYVGRSEGRVRFLWARLDGGLIPEGEAILAVLHEV
                     PRQAVSLGRSIVVDQQQVVTSVMLESGPELPDSGRLRIGAPYPNPYSVSVQEALNIPV
                     HAASGSVEVRILDLLGREIRRIEVSAAGPGSVVHAVWKPTDSNGLPVAPGLYFVRATG
                     ADGSDVRPIVVTR"
     gene            complement(5191..>7688)
                     /locus_tag="AC16YU_09140"
     CDS             complement(5191..>7688)
                     /locus_tag="AC16YU_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6065430.1"
                     /translation="VNPITVTGSRRDERLLDAPASVTVLEARQLNARSVLTAAEHLKD
                     VPGLDLVNTGLVSSRVVIRGFNDNLASSLLTLVDHRIARVPSVRLTALQLIPVSGGDI
                     DQIEVVSGPASSMYGPNAANGVVHLLTRGPFDSKGTTISLAGGERSVRLASIRHAGTV
                     GRRLGYKVTLQSYSGTDFEFFDPEELAARDAAVASGALEDTLRVGARDFDVDNLALSA
                     RVEYRLRPDLTVILDAGMTRGTNIEITPTGAAQVVGAQYTYLQSRLRYRNLFVQAYGN
                     FIDSGDSYFLRTGERFLDQSQLWVAQAQHFSRLGPRQRFTYGIDVFRTLPQGSGTVNG
                     RNEHDDESTEVGVFVQSDTDLHRRLSLSLAARLDHHNRLDNLAFSPRAALVFKPAPAH
                     TFRLTYNRAFQTPKTNDLFSDLIGRRDLFQLGGLESVYGFAPTTDLRVQGMKDGFHFR
                     AGPDHGNQFRSPFAPLMGLAPSDWIGFDDAGFTDVMWSVARFATVAGLAGNLAASGAI
                     DPESAASISAALDQVLPADVEGVHQELRVLDLDAQLFVPVDRATDFGRLGVTRTETVE
                     TGYRGLVFGGLLVSVDAYWTQVRDFLGPFIVGTPNVFLEPLSLSASLRPSIAAALEDP
                     ANAEHAAALQQLDTIPFLGNGNGTPVEEIATLVATGVAGAIPFGTVSPEEAYDPTAVL
                     LMRRNFGDLSVYGMDTAITLFLSRRMRIAASYSLLSRTYFHGVDGVSDVSLNAPRNKA
                     GGMIHYETANGRLETQVRWRFVDSFRVRSDIYTGEVERFGVIDASVGWRLPFVDDTRL
                     SLTVQNLTNNRHREFVDVPEIGRLAILRLTHEL"
BASE COUNT         1179 a         2495 c         2735 g         1279 t
ORIGIN      
        1 gcaaatcgct cggctgctcg cgttctccct ggtaatggtg acactggccg cccggatcgc
       61 cgtccagata cggttcgtgg agaagggcgg gcgggctcgt ctccgtgcgc ggcgacagac
      121 cgaggcctgc cggcttctcg tgcggatcct gggggtccgg gtccgaaccg tcggtgatcc
      181 ccggccgggg gtaccacgcc tcgtcgtgtg caatcactcg ggaatcctgg acggcttcgt
      241 tctcgcgtct gcgatgcccc tggcgaccgt cgccaaggcc gagatggccg ggtggcccgt
      301 catgggatgg gtctgccgca tggtcgggat gatcttcgtc gaccggtccc gcagatcgga
      361 tgccgcgcgc ctggtcgaga ccgtccagga gcgcatgcgc agcggcgtgc cggtgctggt
      421 attccccgaa ggcaccgtag gggacggccg cagtctcctg ccgtttcgct cgggcgcgtt
      481 cgaggccgtc gcggacgcgt cggacatgga ggtgctgccc gtcttcctga cggcaacccg
      541 ggtggacgct gcgagcggtc gcgacgccca ttcggcgctc atctggcatc acggggagtc
      601 gttgatggcc cacgcgcgca gactcctcgg ggcggggcgg atcgacatgg aggtgcggct
      661 cggagagcca atttccacgg ccgggcgtga tcgcaggaac ctggccgagg cgagccgtcg
      721 cggcgtggca cgactgggcg agaccgtact ggatcccacg tgacgggaac gcgttgcggc
      781 ggacaaggta cccacgacga accccgcccg ggaagggcgg gaacgccccc gggcgctccg
      841 gcttcccgtc gggcgtttcg caatcacccg atcggcttca aaatggcccg actgaccgga
      901 cgaaacaccg cgcccctgct gtacgccagc ctggtggtca tcggtctcct gctcggagtc
      961 cttgccacgg tcctggtcgt gcgccccgcg tccgacacgt cgggtgatcg gccggtcgtc
     1021 gaggtggtcc agctgaccag ccgagttgca cccctgccct atgagtccga ttcgctcgga
     1081 gcgaatggct atctcgatgc gctgagcctg aacgaggcct tcaaggcggt gtcgtcccgg
     1141 gtcacgcctt cggtggtatt catcgaagtg gaatcatccg gcggcgggtt cttccatcag
     1201 ttcgaggggg gtgggggatt ccgcctgagc gcgggaagcg gggtcatcgt gtcggacgcc
     1261 ggatacgtcg tcacgaatca ccacgtcatc gaggaagcga cggcgatccg ggtcacgctc
     1321 tccgacaaga gggaattcga cgccgagctc gtgggcgacg atccgaacac cgatctcgcg
     1381 gtgatccgcc tggtcggcgc ggacgcgctc ccgtccatcg tgctgggcga cgcccgatcg
     1441 gtcgaggtcg gggaatgggt cattgcggtg ggcaacccct tccggctgac gtcgacggtg
     1501 accgccggga tcgtcagcgc gatcgggcgg caggtgaaca tcatcaacaa tgacctcgcg
     1561 atcgagaatt tcatccagac ggacgcggcc atcaatcccg ggaattccgg gggagcgctg
     1621 gtcaatctcc gtggcgaact gatcggcatc gcgacggcca tcgcgacgga gtcgggctcg
     1681 tacgaagggt acggattcgc cgtcccggtg gacctgatgc agcgggtcat ctcggatctg
     1741 gtcgagtacg gcgaggtgcg tcgagggtac ctgggcgtca ccatcgagcc ggtgaacgcc
     1801 tccctggccg atgacctcgg catggaccgg gttcgcggag tatttctgcg cgacgtgcgt
     1861 ccggggatgg catcgtggga cgccggcatg cggtccggcg acgtgctggt cgcgatcgac
     1921 gggcggcccg tcgacgaacc caatgcgctc cagggtgcga tcgccctcta caggccgggg
     1981 gaccgggtcc gcgccatcgt ctggcgcgac ggacgggaaa tcgaaatgga cgttgagctg
     2041 ttcggccgcg aggattcccg ggcgagcgag tggttcgcgg agctcgacgg ccggtcggcc
     2101 gatccaggac tggagccgga agaggatgat gccccggccc cgggtgacgg gtcgcctcac
     2161 ggcaacgtac agacgctcga cgagtggggc ctgggcgtca tcgagctttc gtccaggctt
     2221 tcccgcgcct tcgatgtcga cgggggcgtg tacatcatgt acgtggagaa ggacagcccg
     2281 gccgacgcgg cgggcgttcc ccgcaacgcc gtgatcgttt cggtggacgg atcgccgctg
     2341 accgccatcg agaagctcaa gtccgcgctc ggagcggcgg ccgcggcggg gtccgccgta
     2401 gtcctggagg tcgttcgtcg cgacgggacc gtggcgtttt tcgaggtcga agggctggga
     2461 ggctgacgct gccatcgcag ggcgctcgca cggaacctga gtgacgtgag atccggcgtg
     2521 cgggcagggt cggacgaatg gcgactttcg gggttatgtc cccccgcccg tacggttcat
     2581 tcgctcggta tcaccatgcg aggatcccga tgcgagcccg ttacagccag cccgtgccca
     2641 tttccgtcgt cctgcgggac gtcatggagt cctggaagcg tcgtccggat gccgctcccg
     2701 gttccgcgag ggaatcgggc ggcccgccct ccacttcccc ccgccggggc gccacggagc
     2761 ctgcgtcccc cgtactgccg gccggggccg tagcgccggc tcccgggtgt gcgctcccgt
     2821 tcgcgggaat cgccctcgtg atcctgctgc tcgttgccgc tgttccgggc gctgcacacg
     2881 ggcagacggt gtatgtcgaa acattcgacg ccgtcgtcgc acccgacctt cccggcggct
     2941 ggaccgcagg agcagggtgg gcgacgagca cgtcgtcagc atcgacggga agcggcctca
     3001 acaacctggc ccatacgggc atgtcggtgt ctgaggcgac gctgccgccg gtggacctgt
     3061 cgggagtttc gtccgccacg ctgagcttcc tggcgcgccg gacgtcatcg taccccctgg
     3121 agggactgcg cgtcacggcc tcgctggacg gcggagcctc ctggccggtc gagatcatgc
     3181 ccacgggcgc cgccctgccc ggtgcggcaa gtgcgtggga ggccgtcgag gcctcggtgc
     3241 cgatcgctct gatcgggcag agcggcgtcc tggtccggtt cgaagtctcg ggcggaacga
     3301 ccagcggggc gaacctgcgg ctcgacgaca tacggatcgc ggccggaggg ggcggctcga
     3361 cgggtggggg ttccagcctc ggattctcgt ccggatcgag cagcgcgctc tcgccggcca
     3421 ccgacttcac cgtcgccctg cgactcgatt acgatgcgac ggtcggcctg ggtgcgctgc
     3481 agcttcgggt gacctgggac agtgacctgc tcacgtggtc cgggatccta ccgggcgctg
     3541 cggtctccga tcctgcacag tggacggtct cggccgagcc cggcctctcc tcggctatga
     3601 tcctcgtcgt ctcccatgcg gccgcgtcgc tcgtggcggg gacgtacgat ccgatcgtgg
     3661 aactgcgatt cgacgtgggc cccctctccg gcggcgtacc cgaggatgtc accctggcac
     3721 tgtccgacgt aatcggcgcc caggcggtcc cgaccggtga cgatgccggg atcgggacgt
     3781 ccacctccac gcacctggtg accgtgacgc aagccgctcc cgtcttcgag gctccggcct
     3841 ccgtggacct gggcatcgcg gcgatggggc agaccatatc gggcacgatc accgtcacga
     3901 acgcggccgg cggctcggat ctggtcatcg gcagcgtggc ctccgaccac ccctcgttcg
     3961 gcgtgagccc ttcgtccgcc accgtgccgg ctggagcatc gttcgacttc atgctgacct
     4021 acacgccgga cggctctgtg ctcggcgcgg aatcggcgca ggtcagcttt gcgcacgacg
     4081 gttcgaacgg tccggtttct gtcgtatccg tactcgcgcg ggccggggca gggcgcggcg
     4141 acgcgaacgg cgatgcgatg gtcgatcttc tcgactttct gatcgggatc gacgtggcgc
     4201 tcggcaggat cctcccctcc ccgggtgtag tgagcgccct cgacctgtat ccgttcgctt
     4261 caggtgacgg atcggtggac gtccgtgacc tgaccgttct cgctcacgcc atcgtccagg
     4321 acacctggcc ggacggcgag ccactgcccg atccgggagc gggcggcact gcggggaagg
     4381 tcggtggctc cgccggccgg gccaacgcga tcgttgcgct ggccgagaca tcgtccggtc
     4441 tcgaggttcg cgtgagcact cgggtgcccc tgaggggact gcagttcgac ctgctcgtgc
     4501 ccgccagcgt gccggagtgg gaggtcggcg atgccgcggc ggtcgcgccg ctatacgtcg
     4561 ggcggagcga gggcagggtc cgcttcctgt gggctcgcct cgacgggggt ctcatccccg
     4621 aaggcgaggc cattctggcc gtgctccacg aagttccccg tcaagccgtg tccctggggc
     4681 gatccatcgt cgtcgatcag cagcaggtgg tgacgtccgt catgctggag tcagggccgg
     4741 aattgccgga ttcgggacgc ctgcggatag gtgctccgta tccgaatccc tattcggtgt
     4801 ccgtgcagga ggcgctcaac atcccggtcc acgcggcaag cggatcggtg gaggtgcgca
     4861 tcctggacct tctcgggcgg gagatccggc ggatcgaggt gtcggcggcc gggccgggat
     4921 cggtcgtcca tgccgtgtgg aagcccaccg attcgaacgg gcttcccgtg gcgcccggac
     4981 tttacttcgt acgagccacc ggggcggacg gctcggacgt ccgaccgatc gtcgtcaccc
     5041 gctgacggcg gtcaccggcg agcgatccga aggatgacgg cctctttcct gagctcgaaa
     5101 agcgcatcgg aggcgatcca ccgggctccg acctgacgcc acacccaagg tccctagtgt
     5161 atgtcctcct cctcccgatc caacgccgcg ttacagctcg tgggtcagtc gcaggatcgc
     5221 caggcgcccg atctcgggta cgtccacgaa ctcccggtga cggttgtttg tcaggttctg
     5281 gacggtcagc gaaagccggg tatcgtccac gaacggcagt cgccagccga cgctggcatc
     5341 gatcacgccg aagcgctcca cctcgccggt gtagatatcg gaccgtaccc ggaagctgtc
     5401 gacgaaacgc cagcgcacct gcgtctccaa gcgcccgttg gccgtctcgt aatggatcat
     5461 tccaccggcc ttgttgcggg gggcgttcag cgagacgtcg gacacgccgt cgaccccgtg
     5521 gaagtaggtc cgggagagca gcgagtacga ggccgcgatc cgcatgcggc ggctcaggaa
     5581 cagggtaatg gccgtgtcca ttccgtacac ggagaggtca ccgaaattgc gccgcatgag
     5641 aaggactgcg gttggatcgt aggcctcctc gggcgagacc gttccgaacg ggatcgcccc
     5701 ggcgacaccg gtggcaacca gcgtcgcgat ctcctcgact ggcgtgccgt tgccgtttcc
     5761 gagaaacggg atcgtgtcaa gctgttggag ggctgcggcg tgctccgcgt tggccggatc
     5821 ctcgagggcg gccgcgatgg aggggcggag gctggcgcta agcgagagcg gctcgaggaa
     5881 cacgttcggc gtgccgacga tgaagggccc gaggaaatca cgcacctggg tccagtacgc
     5941 gtcgaccgac acgaggaggc ccccgaacac caggccgcgg taccccgttt cgaccgtttc
     6001 agttcgcgtg actcccagtc gcccgaagtc ggtcgcgcgg tcgactggca cgaacagctg
     6061 ggcatccagg tccagcacgc gcagctcctg gtgcacccct tcgacgtcgg caggaaggac
     6121 ctggtcgaga gcggccgaga tggacgccgc cgactcggga tctatggctc cggacgccgc
     6181 caggttgccg gcgaggccgg cgaccgtggc gaagcgggcg acgctccaca tgacatcggt
     6241 gaagccggca tcgtcgaagc cgatccagtc ggacggagcg agacccatca ggggggcgaa
     6301 cggcgatcgg aactggttcc cgtggtcggg gccggcccgg aaatgaaagc cgtccttcat
     6361 gccctggacc cgcaggtccg tggtcggggc aaagccgtag acactttcga gaccacccag
     6421 ctggaacagg tcccttcgac cgatcaggtc ggagaaaagg tcgttggtct tgggcgtctg
     6481 gaatgcacga ttgtaggtga ggcgaaacgt gtgtgccggt gccggcttga agaccagcgc
     6541 ggcgcgcggc gagaacgcga gattgtcgag ccggttgtgg tgatccagcc gcgccgccag
     6601 cgagaggctc agccgccgat gcaggtccgt gtcggactgg acaaagaccc cgacctcggt
     6661 cgattcatcg tcgtgctcgt tgcgcccgtt gacggtgccg gacccctgcg gcagcgtgcg
     6721 gaagacgtcg atgccgtacg tgaaccgctg gcgaggaccg agccgtgaga agtgctgtgc
     6781 ctgcgcgacc cagagctggg actggtcgag gaagcgctcg ccggtccgga ggaagtagga
     6841 gtccccggaa tcgatgaagt tgccgtaggc ctgcacgaac aggttccggt accgaaggcg
     6901 cgactgcaag tacgtgtact gcgcaccgac gacctgggcc gcgcccgtcg gggtgatttc
     6961 gatgttcgtg ccacgggtca tcccggcatc cagtatgacc gtcagatcgg gacgcagccg
     7021 gtactcgacg cgggcggaga gggccaggtt gtccacgtcg aagtcgcgcg cccccacgcg
     7081 gagcgtatcc tcgagagcgc ccgaagccac agccgcatcc cgggcggcga gctcttcggg
     7141 gtcgaagaac tcgaagtccg tgcccgaata ggactgcagg gtgaccttgt agcccagccg
     7201 cctgccgacc gtcccggcgt gccggatgga cgcgaggcga acgctccgtt cgccacccgc
     7261 gaggctgatg gtcgtgccct tcgaatcgaa cggtccgcgg gtcagaaggt gcacgacgcc
     7321 gttggccgca ttcggtccgt acatggacga ggccggcccg gagaccacct cgatctggtc
     7381 gatgtcgccc ccggagaccg ggatgagctg cagggcggtc agccgcacgg aagggacccg
     7441 cgcgatccgg tggtcgacga gcgtgagcag cgaactggcc agattgtcgt tgaagccacg
     7501 gatcaccacg cgcgacgaaa cgagccctgt gttcaccagg tcgagaccgg gtacgtcctt
     7561 caggtgctcg gcggccgtga gcaccgagcg cgcgttgagc tggcgggcct cgagcacggt
     7621 gaccgacgcc ggtgcgtcga gcaggcgctc atcgcggcgc gagcccgtga ccgtgatcgg
     7681 gttcacca
//
LOCUS       DBNHQZ010000342         3413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3624480, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000342 DBNHQZ010000000
VERSION     DBNHQZ010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3624480"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="AC16YU_09145"
     CDS             complement(<1..416)
                     /locus_tag="AC16YU_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HIR6065431.1"
                     /translation="MSETTSATDRANRVVEEFSLFDDWMGRYEHLIDQARYLEPLGDE
                     FRRDEYRIRGCQSQVWLVAAYRDGKVEFRADSDAMITRGLIALLVRVYGGLPPDEIAH
                     ADLWWLDQIGMREHLSSTRKNGLGAMHRRMKEYAAS"
     gene            complement(424..1677)
                     /locus_tag="AC16YU_09150"
     CDS             complement(424..1677)
                     /locus_tag="AC16YU_09150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6065432.1"
                     /translation="MFADRALAEPRTSFDPAVVRADFPLLSGEVNGRPLVYLDNAATS
                     QKPTVVIERMRRYYEAENANVHRGVHTLSQTATDAYEGVRGTVARYLGIPNPAEIVFT
                     RGTTESINLVAQSFARDVLRPGDEIVVTGMEHHSNIVPWQMAAGATGARLRVAPVDDR
                     GELRYEAFEQLLGPRTRLVAVVHVSNSLGTVNPVARIVADAHAMGIPVLVDGAQAVPH
                     GLVDVSGMGADFYCFSGHKVFGPTGAGILYGKMEWLERLPPWQGGGDMIAEVSFERTT
                     FAEVPHKFEAGTPNIAGVVGMGAALEYASAIDWDGASAHEADLLRYAQDSLSEIPGLR
                     LVGTAREKVSVVSFLVGDLHPYDVGTILDRFGVAVRTGHHCTQPLMTRFGIPGTVRAS
                     FALYNNREDVDRLVEAVRAAHRMLT"
     gene            complement(1688..3025)
                     /gene="sufD"
                     /locus_tag="AC16YU_09155"
     CDS             complement(1688..3025)
                     /gene="sufD"
                     /locus_tag="AC16YU_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR6065433.1"
                     /translation="MNKAATPISSTRSEEALVAAWQSVSGRWLNGSNETLHARRADAI
                     EAVRGAGLPRERDEAWKYTNIRRLIDEYADWTLEPPGTPPDRATAERLFVPGLDAYRV
                     VVVDGEIVPGLSQTGELEPGLVVTGLAAASAEFPEVFNRHFGTAREYGRDGLAELNTA
                     FTRDGVFVHAARGAEVRRPVEVLFLSSARDRAVRQPRCLYVADEGSSLRIVEHWRTWG
                     SGRAFTNAVAECIVATGAVVDHVRIVDEADTSVHVHHLEAYQHGGSRFDTNTITLGGG
                     TVRNNAYFLPDGEHCETHLLGLYLGRGDAHYDNHTMVDHAKPECFSNEAYKGILDDRS
                     TGVFNGKVLVRQDAQKINAYQSNRSVLLSETARMFSKPELEIYADDVKCSHGATTGQL
                     DDEALFYLRSRGLRPGTARLIMLQAFAGDVIEQIRDESVRAYVSNRIADLLHS"
     gene            complement(3034..>3413)
                     /gene="sufC"
                     /locus_tag="AC16YU_09160"
     CDS             complement(3034..>3413)
                     /gene="sufC"
                     /locus_tag="AC16YU_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407641.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR6065434.1"
                     /translation="KTKDLGISDDMLKRAVNVGFSGGEKKRNEIFQMAMLEPRLAVLD
                     ETDSGLDIDALRIVADGVNSLRAPDRSFVVITHYQRLLNYIVPDFVHVLVGGRIVKSG
                     GKELALHLEEQGYDWIREDAVSA"
BASE COUNT          522 a         1158 c         1168 g          565 t
ORIGIN      
        1 aacgatgccg cgtactcctt catccggcga tgcatcgcgc cgagcccgtt cttgcgggtc
       61 gaggacaggt gttcccgcat cccgatctgg tccagccacc acaggtcggc atgcgcgatc
      121 tcgtcgggag gaagcccgcc gtacacgcgg acgaggagcg cgatgagccc ccgggtgatc
      181 atcgcgtcgc tgtcggcccg gaattccacc ttcccgtccc ggtacgcggc gacgagccag
      241 acctgcgact ggcatccacg gatccggtac tcgtcccgcc ggaactcgtc tcccagcggc
      301 tcgaggtacc tggcctggtc gatgaggtgc tcgtagcgcc ccatccagtc gtcgaagagc
      361 gagaactcct cgaccacccg gttcgcccga tcggttgcgc tcgtggtctc gctcatggcc
      421 gggtcaggta agcatgcgat gcgcggcacg gacggcctcg acgaggcggt ccacgtcttc
      481 tcgattgttg tacagggcga acgacgcccg cacggtgccc gggatcccga agcgcgtcat
      541 gagcggctgc gtgcagtggt gccccgtgcg cacggccacg ccgaaccggt ccaggatcgt
      601 tcccacgtca tacggatgga gatctccgac gaggaacgac acgaccgaca ccttctcccg
      661 ggcggtgccc acgagccgga gccccggaat ttccgagagg ctgtcctggg cgtagcgaag
      721 caggtccgcc tcgtgggccg aagccccgtc ccagtcgatg gcggacgcat actcgagcgc
      781 cgcgcccatg cccaccactc cggcgatgtt cggcgtgccc gcctcgaact tgtgcggcac
      841 ctcggcgaag gttgtccgct cgaacgagac ctctgcgatc atgtcgccgc caccctgcca
      901 cggggggagc cgctcgagcc actccatctt cccgtacagg atgccggcac cggtaggccc
      961 gaagaccttg tggccggaga agcagtagaa atccgcgccc atgccggaga cgtccacgag
     1021 gccgtgcggc acggcctgcg ccccgtccac cagcacagga atgcccatgg catgcgcatc
     1081 ggcgacgatc cgtgcgacgg gattcaccgt gccgagcgag ttcgagacgt ggacgactgc
     1141 cacgaggcgc gtcctgggac cgagcagctg ctcgaacgcc tcgtagcgga gctcgccgcg
     1201 gtcgtcgaca ggcgccacgc ggagccgggc cccggtggcc ccggcggcca tctgccaagg
     1261 gacgatgttc gagtgatgct ccatgccggt gaccacgatc tcgtcgcccg gccgaagcac
     1321 gtcgcgcgcg aaggactggg ccaccaggtt gatcgactcg gtcgtgccgc gcgtgaacac
     1381 gatctcggcc gggttcggga tgcccaggta ccgggccacg gtgccgcgga ccccctcgta
     1441 ggcgtccgtc gcggtctggc tgagcgtgtg gacgccccgg tggacgttcg cgttttccgc
     1501 ctcgtaatac cgccgcatgc gctcgatcac gacggtcggc ttctgcgacg tggcggcgtt
     1561 gtccaggtag accagcggcc tgccgttcac ttcccccgag agcaggggga agtccgcccg
     1621 cacgaccgcc gggtcgaacg aggtccgggg ttcggcaagg gccctgtcgg cgaacatggt
     1681 gacggtctca ggagtggagc aggtctgcga tgcggttcga gacgtacgcg cgtacggatt
     1741 cgtcgcggat ctgttcgatc acgtccccgg cgaaggcctg gagcatgatc agccgggccg
     1801 tgcccggacg caggccgcgc gagcgcaggt agaagagcgc ctcgtcgtcg agctgtcccg
     1861 tcgtggcccc gtgactgcac ttcacgtcgt cggcatagat ctcgagctcg ggcttggaga
     1921 acatccgggc cgtctcggaa agcagaacgc tcctgttgga ctggtaggcg ttgatcttct
     1981 gcgcgtcctg gcgtaccagg accttgccgt tgaagacccc ggtggatcgg tcgtccagga
     2041 tgcccttgta ggcctcgttc gagaagcatt cgggcttcgc gtggtcgacc atcgtgtggt
     2101 tgtcgtagtg cgcgtcgccg cggcccaggt acaggccgag caggtgcgtc tcgcagtgct
     2161 cgccgtcggg aaggaagtac gcgttgttgc gcaccgtccc gccgccaagc gtgatcgtgt
     2221 tcgtgtcgaa gcggcttccg ccgtgctggt aagcctccag gtggtggacg tggaccgagg
     2281 tgtcggcctc gtcaacgatc cgcacgtggt ccaccaccgc gccggtcgcg acaatgcact
     2341 cggcgacggc attcgtgaac gcgcggccgg aaccccatgt gcgccaatgc tcaacgatcc
     2401 ggagcgacga gccttcgtcc gccacgtaca ggcagcgggg ctgccggacg gcccggtccc
     2461 tggcggacga gaggaagagc acctcgacgg gccgtcggac ctctgcgcca cgggctgcgt
     2521 gcacgaagac gccgtcccgc gtgaacgccg tgttcagctc ggcgaggccg tcgcggccgt
     2581 actcccgggc ggtgccgaaa tgccggttga agacctcggg gaactcggcc gaggcggcgg
     2641 cgaggccggt gacgacgagt cccggctcca gctcgcccgt ctgcgagagc ccggggacga
     2701 tctcgccgtc gacgacgacg acgcggtagg catcgaggcc cgggacgaag agccgttcgg
     2761 ccgtggcccg gtcgggcggg gtgcccgggg gctcgagggt ccagtcggcg tactcgtcga
     2821 tcaggcgccg gatgttggtg tacttccacg cctcgtcccg ctctcgcggc agcccggcgc
     2881 cgcgaacggc ttcgatcgcg tccgcgcgcc tcgcatggag cgtctcgttg gagccgttga
     2941 gccacctgcc gctcacggac tgccatgccg caacgagggc ctcctcggac cgggtggacg
     3001 atatgggggt cgctgccttg ttcatggagt ccctcaggcc gagacggcgt cttccctgat
     3061 ccagtcgtat ccctgctcct caaggtgcag ggcgagttcc ttgccgcccg acttcacgat
     3121 ccgtccgccc accagcacgt ggacgaagtc cggcacgatg taattgagca ggcgctggta
     3181 gtgcgtgatg acgacgaacg atcggtcggg cgcccggagg ctgttcacgc cgtccgccac
     3241 gatgcgcagc gcgtcgatgt ccaggcccga gtccgtctcg tcgaggacgg cgaggcgcgg
     3301 ctcgagcatg gccatctgga aaatctcgtt ccgcttcttc tcgccgcccg agaagccgac
     3361 gttgacggcg cgcttcagca tgtcgtcgga gatgcccaga tccttcgtct tct
//
LOCUS       DBNHQZ010000343         8083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_373780, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000343 DBNHQZ010000000
VERSION     DBNHQZ010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_373780"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..780)
                     /locus_tag="AC16YU_09165"
     CDS             complement(181..780)
                     /locus_tag="AC16YU_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/polyprenol kinase family protein"
                     /protein_id="HIR6065435.1"
                     /translation="MTDSATLAFRGEVARKTLHLASLALPGAMLTLPYAAVLVSLVAL
                     SAVAISIEIARDRSAAIHARIDRWLGWMMRPVERRPGSGFCGATWVVIAATLLLAAFP
                     PAIAAASMAIGLVGDAAAALVGRALGRHAWPGTSRTAEGTAGFLVAGMLVAVLFGGFP
                     WSARIAAVVAAAVVEALPVPLNDNLVVPFAAAAALALLG"
     gene            863..1708
                     /locus_tag="AC16YU_09170"
     CDS             863..1708
                     /locus_tag="AC16YU_09170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6065436.1"
                     /translation="MRALSSCLLPLVLLAAAACGGGDDHDRRMEREHAGETPTATDAT
                     RGLLGTAVGEDVVYAAGAGTTATGYLSLPEAGASGIPGVIVIHEWWGLNDNVRRLTDR
                     LAEAGYAALAVDLYGGVVAEDSELASELSQKVRGSMDAARANLVGAYAFLADSLGAPT
                     VGSVGWCFGGMMSLEAAMELPEELDAAVIYYGRTTDDRQRLGPLQLPILGHFGGEDEG
                     IPLDSVRAFEAAMTELGKEIQIHVYEGAGHAFANPSGQAYQPEAAETAWARTTAFLAE
                     HLGSR"
     gene            1785..2858
                     /locus_tag="AC16YU_09175"
     CDS             1785..2858
                     /locus_tag="AC16YU_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6065437.1"
                     /translation="MPDPTPITGLHHITLVSGDAQRTVDFYAGVLGLRLVKQTVNFDD
                     PGSYHLYFGDDAASPGSLVTFFEWPDAPRGRPGIGGTHHFALSVDGDDGLLKWKRRLT
                     DAGRHVTGPFDRRYFRSIYFTDPDGQVVEIATREPGFTVDEASGVLGEGFVEPPDPAS
                     AAPGDATWDEPVPAITDGMRLSTGMHHVSIIVSDIGRAHAWYGDLLGLSRIKQTANFD
                     DRRVAHWYWGLDGGRPGTLVTAFEWKHEGAATARIGRGQTHHLALGVEDDQAQEAWRR
                     RILASGVPVSPVRDRVYFRSIYTQDPDGHIFELATAGPGFAVDEPARALGTSLKLPPW
                     LDSLRTAIEGKLKPLVVPENSHG"
     gene            2851..3492
                     /locus_tag="AC16YU_09180"
     CDS             2851..3492
                     /locus_tag="AC16YU_09180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6065438.1"
                     /translation="MGNATDRILGPEPAEAESVLILLHGRGASARGLQPLAAAVAPAG
                     TSVLAPQADGNTWYPNRFLLPRTTNEPWLSNALARVETLVRRVEQAGVPRDRVVVAGF
                     SQGACLAVEHVARHPARYAGCVAIAGALIGPDPLADDYAGDLAGTPVHLAVGDRDEHV
                     PPRFVEDSAEVLERMGARVTVRIHRGMGHDIHPDEVQALAALLRKAGGPGVGA"
     gene            3489..3836
                     /locus_tag="AC16YU_09185"
     CDS             3489..3836
                     /locus_tag="AC16YU_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR6065439.1"
                     /translation="MTLESLYTYCASLPGAEETFPFGPDTLVMKVGGKMFALVGLESM
                     PMLVNLKCDPERALELRASYEGIEPGWHMSKIHWNSVYLASDVPAELIRELVRHSYDL
                     VVASLPAAKRSVG"
     gene            4131..5624
                     /locus_tag="AC16YU_09190"
     CDS             4131..5624
                     /locus_tag="AC16YU_09190"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HIR6065440.1"
                     /translation="MKRYSSLLLLVTGALILSTGSVLFSPGPTLTPMEQLGKELFFDR
                     ISSPDRISCATCHAPRYGFTGPNDGINRRGSVYRGAIPQRFGNRKPPSAAYATLSPVF
                     HFDEDEGLFVGGNFWDGRATGWHLGNPAADQALGPFLNPVEQNNPSPLAVLEQVAASR
                     YAGLWELAWGEPLSTDPADVALNYERVGLAIAAYEGSPEVNAFTSKYDYYLAGEVEFS
                     EQEAWGLELFEGKAMCSACHPSEPGPQGEPPLFTDFTFDNLGTPRNPLNPFYDMDEVY
                     LDDGSPINPMGDAWIDPGLGGFLATVPEWAHLAAENMGKHKVPTLRNVAKGAQSDQGK
                     AFVHNGVFTSLRDVVHFYNTRDVEDWPEPEVAENVNVDELGNLGLTADEEAAIVAFME
                     TLSDGYVVGTKGKSEMATVARASSSAHPNPFNPTTTITYVLPEAADVRLAVYDMLGRE
                     VRLLADGAASAGTHAARFDASGLPSGVYFYRLQVGDFAESHQLVLTK"
     gene            5706..6401
                     /locus_tag="AC16YU_09195"
     CDS             5706..6401
                     /locus_tag="AC16YU_09195"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6065441.1"
                     /translation="MTLPVLYLDNHLLVVVKPPGMLSQADRTGDADVLSIGKALLKER
                     FDKPGNVFLGLVHRLDRPASGVMVLARTSKAAARLAEQFRARSVGKEYLALVEGTLEG
                     GGAWTDFLVKEDEAVRRAAEGEPGAKEARLRWTAISSGPDTSLVQVALETGRAHQVRV
                     QFAERGHPLVGDFRYGSRRPLDGRNLALHCVRLSIDHPTRGDRMAWSAPPPDSWPPDA
                     REAAARWIGTREG"
     gene            6585..7817
                     /gene="hemH"
                     /locus_tag="AC16YU_09200"
     CDS             6585..7817
                     /gene="hemH"
                     /locus_tag="AC16YU_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6065442.1"
                     /translation="MLTPREYLRSFHRAGRPGGGLPFPDERMDVPAGSTVGVVLLGPG
                     GPQTHAEVPAFLYRQYMDPAVAEVRFGSWIQHVISRVAAKRRAAVARREYEAIGGRAP
                     MLRHVGELAFMLQRELESAGSPRSVRFRVYLAMRYGEPSPQGAIRRMRADGIRHVVLL
                     PLHPQFSEATTGSSLAYWQAAVEREGEEFRTVVVRAYPDEPDFVQAISERIDEGLQRF
                     PRAVRRDVKVVFVAHGYAPRGRRDRCAPYCCHVHRTVDAVMRLRGHDRPFEVAFQSQP
                     GLPVRLTPLLVDAVRRMGDGGQRAVLLVPVSFVTEQVDTAYRLDVTVRREAESCGIAH
                     YEVAMPLNCHPLFVRALADVTEERVATEGVPLNARERSAGEERCPRGVWNGPSALGAG
                     FDERCSRCEFAEGSIGRA"
     gene            complement(7831..>8083)
                     /locus_tag="AC16YU_09205"
     CDS             complement(7831..>8083)
                     /locus_tag="AC16YU_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065443.1"
                     /translation="FEPRLVILDEPFANLDPPSQIRLKLLLRQMHQRTGTTMLISSHD
                     LGHVTELCSRITLIDHGKVVSDAATTPGSLKELEQYFSY"
BASE COUNT         1195 a         2801 c         2929 g         1158 t
ORIGIN      
        1 cggagcgacg cgagcaggcc gaagccgtgc atcagcacga tggggtagcg cgtgcggatg
       61 acgggcggtt ggggaaatgc gtcgaggccg gccaggcggc gcagggtctg cttgccgatc
      121 gagggcatgg ttgctgcggt tgcggccgaa aggtacggca cggacctcgc cccgggtgcg
      181 tcagccgagg agcgcgagcg ccgccgccgc cgcgaagggc acgaccaggt tgtcgttcag
      241 cggaacggga agcgcctcga ccacggccgc ggcgacgacg gccgcgatcc tggcactcca
      301 ggggaaaccc ccgaagagga ccgcgaccag cattccggcc accaggaagc cggccgtccc
      361 ctccgcggtc cgactcgtgc ccggccatgc gtgacgcccg agcgcacggc cgaccagggc
      421 cgccgccgca tcgcccacga gcccgatcgc catcgaggcc gccgcaatgg cgggcgggaa
      481 cgcggccagc agcagcgtgg ccgcgatcac cacccaggtg gccccgcaga aaccgctgcc
      541 ggggcggcgt tccacgggtc gcatcatcca tccgagccac cggtcgatcc gggcgtggat
      601 ggcggcactg cggtcgcgcg cgatctcgat cgagatggcg acggcggaca gggcgacgag
      661 cgacacgagg accgcggcat acggcagggt cagcatcgcc ccgggcagcg cgagggaggc
      721 caggtggagc gtcttccggg cgacttcgcc gcgaaatgcc agggtggcgg agtcggtcat
      781 gggcgggcgg caatgggcac tcgggggcac atgcaggggc gtggcgcgta aacccgatcg
      841 caaactaatc cggatcacgg acatgcgtgc cctttcttcc tgcctgctac ccctggtgct
      901 gctcgcggcc gcggcctgcg gcggcggtga cgaccacgac cggcgcatgg agcgcgagca
      961 cgccggagag acgccgacgg cgaccgacgc cacgcgcggc ctgctcggga ccgccgtggg
     1021 cgaggatgtc gtctacgcgg cgggtgcggg gacgaccgcc accggctacc tgtccctgcc
     1081 ggaagccgga gcttcgggga tcccgggcgt gatcgtgatc cacgagtggt ggggcctcaa
     1141 cgacaacgtg cggcgcctga ccgaccggtt ggccgaggcg ggatacgccg cgctcgccgt
     1201 cgacctttac ggcggcgtgg tggcggagga ctcggaactc gcaagcgagc tttcccagaa
     1261 ggtccggggc agcatggatg cggcgcgcgc caacctggtg ggtgcgtacg ccttcctggc
     1321 cgactcgctg ggcgcgccga ccgtggggtc ggtggggtgg tgcttcggcg gcatgatgtc
     1381 gctcgaggcg gccatggagc tcccggagga actcgacgcg gccgtcatct actacggacg
     1441 gacgaccgac gaccggcagc ggctcggccc gctgcagctt ccgatcctcg gccacttcgg
     1501 cggcgaggac gagggcattc cgctcgactc cgtgcgggcc ttcgaggcgg caatgacgga
     1561 gctcggaaag gagatccaga tccacgtcta cgagggcgcc gggcacgcct tcgcgaaccc
     1621 ctcgggccag gcgtaccagc cggaggcggc cgagacggcc tgggcgcgga ccacggcctt
     1681 tctcgcggag cacctcggca gccggtaatc cgggggcccg gatcgttgga cgggagacct
     1741 cccggcccta tattacttgt tgcaacaaga aactgcgggg gcgtatgccc gatccgacgc
     1801 cgatcaccgg cctgcaccac atcacgctcg taagcggcga tgcccagcgc acggtggatt
     1861 tctacgccgg ggtgctcggt ctccggctgg tgaagcagac cgtgaacttc gacgatccgg
     1921 gcagttacca cctgtatttc ggggacgacg cggccagccc aggctcgctc gtcacgttct
     1981 tcgagtggcc cgacgccccg cgcggccggc ccggcatcgg gggcacgcat cacttcgcac
     2041 tctctgtcga tggtgacgac ggtctgctca agtggaagcg gcgccttacg gacgccggac
     2101 gccacgtgac ggggcccttc gaccggcggt acttccgttc gatctacttc accgaccccg
     2161 acggccaggt ggtcgagatc gcgacgcgcg agccgggatt cacggtggac gaggcgtcgg
     2221 gcgtgctggg cgagggcttc gtcgagcctc cggacccggc ctcggccgcc cccggcgacg
     2281 ccacctggga cgagcccgtc cccgcgatca cggacggcat gcgcctgtcg accgggatgc
     2341 accacgtctc gatcatcgtc tcggacatcg ggcgcgcgca cgcctggtac ggcgacctgc
     2401 tgggcctgtc ccggatcaag cagacggcga acttcgacga ccggcgagtc gcccactggt
     2461 actgggggct ggacggcgga cggccgggga ctctggtcac cgcgttcgag tggaagcacg
     2521 agggcgcggc gaccgcgcgg atagggcggg ggcagaccca ccacctggcg ctcggcgtcg
     2581 aggacgatca ggcccaggag gcctggcgca ggcgcatcct ggcctcgggg gtccccgtgt
     2641 cgccggtgcg cgaccgggtg tacttccgct cgatctacac gcaggacccg gacgggcaca
     2701 tcttcgaact ggcgaccgcg ggccccggat tcgccgtgga cgagcccgcc cgcgcgctcg
     2761 gcacctccct taagctcccg ccctggctcg attccctgcg gacggccatc gaggggaagc
     2821 tgaagccgct cgtggtcccg gagaattccc atgggtaatg cgacggaccg gatactcggc
     2881 ccggagccgg cggaagcgga gagcgtcctg atcctgctgc acgggcgggg cgcgtcggcc
     2941 cgcggccttc agccgctggc ggcggcggtg gcgccggcgg gcacaagcgt tctcgcgccc
     3001 caggccgacg gcaacacctg gtatccgaac cgcttcctgc tgccgcgcac gacgaacgag
     3061 ccctggctgt cgaatgccct ggcgcgcgtc gagaccctgg tgcggcgggt ggaacaggcc
     3121 ggcgtcccgc gcgaccgcgt ggtcgtcgcc ggcttttcgc agggcgcgtg cctcgcggtg
     3181 gagcacgtgg cgcggcaccc cgcccggtat gccggatgcg tcgcgatcgc gggggcgctg
     3241 atcggcccgg accctctcgc cgacgattac gccggcgacc tcgcgggtac gcccgtgcac
     3301 ctggccgtcg gcgaccgcga cgagcacgtg ccgccacgat tcgtcgagga ctcggccgag
     3361 gtcctggagc ggatgggggc ccgcgtcacg gtccgcatcc accggggcat ggggcacgac
     3421 atccatccgg acgaggtgca ggccctggcg gccctgctcc gcaaggcggg cgggccgggg
     3481 gtgggagcat gaccctcgag tccctgtaca cgtactgcgc cagcctcccc ggcgcggaag
     3541 agaccttccc gttcgggccc gacacgctcg tcatgaaggt gggcgggaag atgttcgccc
     3601 tcgtcgggct cgaatcgatg ccgatgctcg tcaatctcaa gtgcgatccg gaacgggcgc
     3661 tggagctcag ggcgtcgtac gagggcatcg agccgggctg gcacatgagc aagattcact
     3721 ggaactcggt gtacctggcg tccgacgtgc ccgcggagct catccgggag ctcgtgcgcc
     3781 actcctacga cctggtggtg gcgagcctgc cggcggccaa gaggtccgtg ggctgacgaa
     3841 cgatcccacg gctccgtggg agggccgccg ccgagtaatg gtccgggtcc ccacgccggg
     3901 cgccgggtcg tacgccatcg gtgccctccc cgtaggaccc gcccctacag cctgccgtga
     3961 agcggccccg tcatccggga gccccgggaa ccgctaaaaa gcctgtaccc gttcggccga
     4021 tgccggggct cgagcgcggg cggcggttcc tcccgcccgc cggaagcaca ggggaggcac
     4081 gctccgaccg gggatgcact gtcccgaccc cctccggagg tactcccacc atgaaacgtt
     4141 acagttccct tctgctgctc gtcaccggcg cactgatctt gagcaccggc tcggtcctct
     4201 tctcgcccgg accgactctt acccccatgg aacagctcgg caaggagctc ttcttcgaca
     4261 ggatctcgtc ccccgaccgg atatcgtgcg ccacgtgcca cgctccgcgc tacgggttca
     4321 ccggtcccaa cgacggcatc aacaggcggg gctccgtgta ccgcggcgcg atcccgcagc
     4381 ggttcggaaa ccggaagccg ccgtccgcgg cctacgcgac gctcagcccg gttttccact
     4441 tcgacgagga cgaaggcctt ttcgtgggcg gcaatttctg ggacggccgg gcgaccggct
     4501 ggcacctggg caatcccgcg gccgaccagg cactgggtcc cttcctgaat ccggtggaac
     4561 agaacaaccc gagcccactg gccgtgctcg agcaggtggc cgcgtcgagg tacgcggggc
     4621 tctgggagct ggcatggggt gagccgctca gcacggatcc cgccgacgtg gcgttgaact
     4681 acgaacgcgt cggactcgcc atcgcggcct acgagggatc gccggaagtc aacgcgttca
     4741 cgtcgaagta cgactactac ctggccggcg aggtggagtt ctcggagcag gaggcctggg
     4801 gcctggagct gttcgagggc aaggccatgt gctcggcctg tcatccgagc gaaccgggtc
     4861 cgcagggcga gcccccgctc ttcaccgact tcacgttcga caacctgggc acgcccagga
     4921 accccctgaa cccgttctac gacatggacg aggtctacct cgacgacggc agcccgatca
     4981 acccgatggg cgacgcatgg atcgacccgg gcctcggggg ctttctcgcg accgtgcccg
     5041 agtgggccca cctggccgcg gagaacatgg gcaagcacaa ggtgccgacg cttcgcaacg
     5101 tggcgaaggg cgcgcagagc gaccagggca aggccttcgt gcacaacggc gtgttcacgt
     5161 cgctccggga cgtggtccat ttctacaata cccgtgacgt ggaggactgg cccgagcccg
     5221 aggtggccga gaacgtgaat gtcgacgagc tcggaaacct gggcctgacg gccgacgagg
     5281 aggcggccat cgtggccttc atggagaccc tctcggacgg ctacgtggtc gggacgaagg
     5341 ggaagagcga gatggcgacc gtggcgcggg cgagcagctc ggcgcatccg aacccgttca
     5401 acccgacgac caccatcacg tacgtgctgc ccgaggccgc cgacgtacgc ctggccgtct
     5461 acgacatgct cggccgcgag gtgcggctgc tcgccgacgg cgccgcgtcg gccggcacgc
     5521 atgcagcccg gttcgacgcg agcggcctgc cttcgggcgt gtacttctac cgcctgcagg
     5581 tcggtgactt cgcggagagc caccagctcg tgctgacgaa gtagcccggg gcacacccgc
     5641 ccggacggat acgccccggc cctcgatttc gcgggtcggg gcgttgccgt gtcatagatt
     5701 cggccatgac gcttcccgtc ctgtacctgg acaaccatct gctggtggtg gtgaagccgc
     5761 ccgggatgct gtcgcaggcg gaccggacgg gcgacgccga cgtcctttcg atcggcaagg
     5821 ccctcttgaa ggagcgattc gacaagccgg gcaacgtgtt cctggggctc gtgcaccggc
     5881 tggaccgccc ggcctccggc gtcatggtgc tcgcgcggac gtcgaaggcg gcggcgcggc
     5941 tcgcggagca gttccgcgcg cggagcgtcg gcaaggagta cctggcgctc gtcgagggga
     6001 cgctcgaagg cggcggcgcg tggacggatt tcctggtcaa ggaggacgag gccgtgcggc
     6061 gcgccgcgga gggcgagccg ggcgcgaagg aggcccgcct gcgctggacg gcgatctcga
     6121 gcggtcccga cacctcgctc gtgcaggtcg cgctcgagac gggccgcgcc caccaggttc
     6181 gcgtgcagtt cgcggagagg ggacacccgc tcgtcgggga tttccggtac ggaagccgcc
     6241 gcccgctcga cggacgcaac ctcgcgctgc actgcgttcg cctctcgatc gaccatccca
     6301 cgcggggcga tcgcatggcc tggtcggcac cgccgccgga ctcctggccg cccgatgccc
     6361 gcgaggcggc ggcccgctgg atcggtaccc gcgagggctg accggcccgg gccccgcgcc
     6421 gccggaccgt gaatccgccg tgcggaaggg gtgaagcttc gcgcaatacc cgtccggcac
     6481 gcgagcgtac ccccgggagg cccctgagtc tcggtacctt cgggtagccg ccgcccttcc
     6541 gagggtgacg tgtctccgtc cgaaaccgcc tgctgcccgg acccgtgctc accccccgcg
     6601 aatacctccg ctccttccat cgtgccggcc ggcccggcgg tggactgccg tttccggacg
     6661 agcggatgga cgttccggcc ggatcgacgg tgggcgtggt gctcctcggg ccgggcggcc
     6721 cgcagacgca cgccgaggtg ccggccttcc tgtaccgcca gtacatggac ccggccgtgg
     6781 cggaggtccg gttcgggtcg tggatccagc acgtcatcag ccgcgtggcg gcgaagcgcc
     6841 gcgccgccgt ggcccgccgg gagtacgagg cgatcggcgg ccgggcccca atgcttcgac
     6901 acgtagggga gttggccttc atgctccagc gcgagctcga gagtgcgggc tcgccgcgct
     6961 cggtcaggtt ccgcgtatac ctggccatgc gctacggcga accgtcgcct cagggcgcga
     7021 tccgcaggat gcgggcggac ggcatccggc acgtggttct gctcccgctg cacccgcagt
     7081 tctcggaggc gacgaccgga tcgtcgctcg cctactggca ggccgccgtc gagcgcgagg
     7141 gcgaggagtt ccggacggtc gtggtccggg cgtatcccga cgagcccgat ttcgtgcagg
     7201 cgatctccga gcggatcgac gagggcctgc agcggtttcc gcgcgcggtc cgtcgcgacg
     7261 tgaaggtcgt cttcgtcgcc cacgggtacg ccccgcgcgg ccgaagggac cgctgtgccc
     7321 cgtactgctg ccacgtgcac cgcaccgtcg acgcggtcat gcggctgcga gggcacgacc
     7381 ggcccttcga ggtggccttc cagtcgcagc ccgggcttcc ggtccggctc acgccgctcc
     7441 tcgtcgacgc ggtccgccgg atgggggacg ggggacagcg ggccgtgctc ctggtcccgg
     7501 tttcattcgt caccgagcag gtcgacacgg cctatcgcct ggacgtcacg gtccgccgcg
     7561 aggcggagag ttgcgggatc gcgcactacg aggtggcgat gccgctcaac tgccatcctc
     7621 tcttcgtccg ggcgctcgcc gacgtcacgg aggagcgcgt cgcgacggaa ggcgtgccgc
     7681 tgaatgcacg cgagcggtcc gccggcgagg aacgctgtcc gcgcggcgtc tggaacgggc
     7741 cctccgccct gggagccggg ttcgacgagc gctgcagccg gtgcgagttc gccgagggct
     7801 cgatcgggcg ggcctgagcg gccggagcgc tcagtacgag aagtactgct ccagctcctt
     7861 gagcgagccg ggcgtcgtcg cggcgtcgga cacgaccttg ccgtggtcga tcagcgtgat
     7921 ccggctgcac agctcggtca cgtggcccag gtcgtggctc gagatgagca tcgtcgtgcc
     7981 ggtgcgctgg tgcatctgcc ggagcaagag cttcagccgg atctgcgacg ggggatcgag
     8041 gttcgcgaac ggctcgtcca ggatcacgag ccgcggctcg aag
//
LOCUS       DBNHQZ010000344         7194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2134425, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000344 DBNHQZ010000000
VERSION     DBNHQZ010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2134425"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="AC16YU_09210"
     CDS             complement(<1..1034)
                     /locus_tag="AC16YU_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6065444.1"
                     /translation="MRSRSSQDPPTGAPGAAGNAPADRHERAGGGLSGVAIRRPVFTG
                     MMMMALVVLGVFSFRRLPIDLFPEIDIPVVAIQTIYPGASPETIEREVTQRLEEAFNP
                     VQGVDRITSTTLEGASQIVIEFELDRDVDQAAQDVRDKIAAVRRELPADIEEPLVLKF
                     DIAAQPIISLTLSSSTMPIADLTALADETVRRRLESVTGVGQAQIAGGQEREIRVNLV
                     PDRMQARGISVTDVMGALQMQNMEVPAGRLERDNTEQLVRVTGRIEDPAGFGAIIVAN
                     RNGTAIRLRDVARIEVGTEEERSVALVNDRRAVAVDVLKVSGANTVAVADGVLEAVAE
                     LDELLPEGTTL"
     gene            complement(1037..2269)
                     /locus_tag="AC16YU_09215"
     CDS             complement(1037..2269)
                     /locus_tag="AC16YU_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6065445.1"
                     /translation="MIRISMNGFTMARTARLRVPRLRPPRLRVLARLPAALLLGFVLA
                     GAAGCGSASDTDSSAAGTEGLEGVVLGERDVATVTRGTLEEGITLTGTLEPYRRVDVK
                     SQVGGTLARLGADRGDAVAQGRVLAVIDAEGILSQAASAQAGVAAARAALALAVRQLE
                     SARTLYEAGALSEIEYEQAKAQHEAAQAQLSAAQAAEAMAAEQAAHSTVRSPIGGVVS
                     DRLVETGEAIDRNQTLFTVVNTSRLELNGQVPVSEAIRLEVGQGVVFTLDALPGETYR
                     GEVARIEPTADPGTRQVGVYLAMANPGGLVGGLFATGRVLSGAAREVLLVPVAALRGP
                     AGEEHVFVVESGQIVRRPVAVNGRDERRGVVGVEGPLREGDIVLVSPGANIEEGMSVR
                     LSSRPAGAAVITDSSEVQ"
     gene            complement(2285..3772)
                     /locus_tag="AC16YU_09220"
     CDS             complement(2285..3772)
                     /locus_tag="AC16YU_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6065446.1"
                     /translation="MRISTLCRALVLVAGVWMPAAATAQTAATQEAARPAARADSLDL
                     SLDGALERALASSEEVMLARSQVDLAAARVRDAWSAALPQVTGSAGYTRTVKSAFDTG
                     GGDFELPDSLKFEPDPTASIEDRIAYLEDKTPNAAFGALAQLFGDLPFGQKNAYTFGI
                     SGSQLLYSGGRTGAGLRIASAVADAARLNLREQTAAIELDVRTAYYRALFARELVGIS
                     GDALERARRFLDEERLRFDAGRASDLDVMRAQVELENLRPQLVEARNAAELAELNLKR
                     LVNVPLEQPLRLVTRLESPPAEGRVDVRLDPEVLGAQRASVQALREQVRIREQQVRIA
                     RSGYLPTVSFNTSYGKLIYPSTLFAFGEQWRTDFTLSLNVQIPIFDGFRRTAQVQGAR
                     VELHQAQLQVRQLEEAIQLEYEQALGEKQRALAQIDAREATVDQARRVYDLTEMRYEQ
                     GLATQLDVFSALLSLNQARVNLARALTDYYVADAGVDRAMGGNLQ"
     gene            complement(3786..4262)
                     /locus_tag="AC16YU_09225"
     CDS             complement(3786..4262)
                     /locus_tag="AC16YU_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR6065447.1"
                     /translation="MHDAVAAFIEQMGLVSEANGLSRIAGRMIGLLLVDGRTHSLDEI
                     VERLRVSKGSVSTNARMLAQHGIIERRSVPGDRRDYYRIGDRPWEQMLEMIRQRMRRT
                     LRAVEKGLDEIPEDLADARRRLETWHGFYSFLLDDFEHTPERWARWLAEHPERATS"
     gene            complement(4522..5265)
                     /locus_tag="AC16YU_09230"
     CDS             complement(4522..5265)
                     /locus_tag="AC16YU_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065448.1"
                     /translation="MNLKRKPLFVSLLLDETGSMQSIKDDTIRAYNKYLEGLKESKSD
                     SDILFSLVTFNSSRTQTRVVAEKVQSVGPLTTEDYRPNAMTPLIDAAVKIIRATDEAV
                     AARGDSPDVVVVIQTDGLENCSVEHDAGDLALLVKEKEQAGWQFVFLGAGLDAFDAAR
                     HAGLSLREDVVMSYGREHSEEAMDILAEKTRAYDELRSPAALAFNVGDRRRAGDRWHK
                     ANPADPDPADPKPTKKSDRRSSAGDIKLT"
     gene            complement(5249..5566)
                     /locus_tag="AC16YU_09235"
     CDS             complement(5249..5566)
                     /locus_tag="AC16YU_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6065449.1"
                     /translation="MPESFGDFLQRLRRQKGLTQRHVANRLGIEPTYLSKVENDVEGY
                     SSLSEEKLVALSRLLEADSDEVLARAGKVPSDVRRMLVDDISLIKEIRSRYNEDPTNE
                     SEA"
     gene            complement(5768..6469)
                     /locus_tag="AC16YU_09240"
     CDS             complement(5768..6469)
                     /locus_tag="AC16YU_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065450.1"
                     /translation="MKSFIYGTAAGAVGALAWAGIAYATDYEVGYVAIAVGALVGLAV
                     AAGSRESRRSPTALGVMAVILAMLSIVAGKIAMVDLFIPDDDEIVAMYVESYQDEEFV
                     MSFIADAVVEEYVDAGRQVDWPVGVDPSTASAATDYPSDVWAETVQRWSEMTEDEQEA
                     FGEARLSEVRANLEASLPAIRASMGREGLFASFSAIDLIFFGLAMLYAWRFGSGTWGT
                     ANEGPRVDPSPDARS"
     gene            complement(6697..>7194)
                     /locus_tag="AC16YU_09245"
     CDS             complement(6697..>7194)
                     /locus_tag="AC16YU_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065451.1"
                     /translation="WRASFGPGAFQVAARALDPALPGPGPAMPLSGIGQAQAPDAAHR
                     ALRDEVLLVWEDGDIFGRRWSTAGGVATEPIPHVGAPVLEPAFPNPFGDRTTIPFRLE
                     KAGSVRLEVVDLLGRTVARLVDAQLPPGAHAAELDAAALPPGAYLVRLESGGAVSTRM
                     VMRVR"
BASE COUNT         1050 a         2546 c         2484 g         1114 t
ORIGIN      
        1 agggtcgtgc cctccgggag cagctcgtcg agctcggcga cggcctcgag cacgccgtcg
       61 gccacggcca ccgtgttcgc gcccgacacc ttcaggacgt cgacggcgac ggcgcggcga
      121 tcgttgacga gggcgaccga gcgctcctcc tcggtgccga cctcgatgcg tgcgacgtcc
      181 cggagccgga tcgccgtgcc gttccggttg gcgacgatga tcgcgccgaa cccggccggg
      241 tcctcgatgc ggcccgtgac ccggaccagc tgctccgtgt tgtcgcgctc gaggcgaccg
      301 gcgggaacct ccatgttctg catctgcagc gcgcccatga cgtccgtcac cgagatcccg
      361 cgcgcctgca tccggtcggg caccaggttc acgcggatct cgcgctcctg tccgccggcg
      421 atctgcgcct ggccgacgcc ggtgacgctc tcgaggcgcc gccggaccgt ctcgtcggcc
      481 agcgccgtga ggtcggcgat cggcatcgtg ctcgacgaga gcgtcagcga gatgatgggc
      541 tgggccgcga tgtcgaactt gagcacgagc ggctcctcga tgtcggccgg aagctcgcgc
      601 cggacggccg cgatcttgtc gcgcacgtcc tgggcggcct ggtccacgtc gcgatccagc
      661 tcgaactcga tgacgatctg cgaggcgccc tcgagcgtgg tcgacgtgat gcggtcgacg
      721 ccctggacgg gattgaacgc ctcctcgagg cgctgcgtca cctcgcgctc gatcgtctcg
      781 gggctggccc cgggatatat ggtctgtatg gcgacgaccg ggatgtcgat ctccgggaac
      841 aggtcgatcg ggaggcgccg gaagctgaag acgcccagca cgaccagcgc catcatcatc
      901 atgcccgtga agacgggcct ccggatggcg acgccgctga gtcccccgcc ggcacgctcg
      961 tgccggtccg cgggcgcatt cccggcggcg ccgggtgcgc ctgtcggcgg gtcctggctg
     1021 ctcctgctgc gcatcctcac tgtacctccg atgaatccgt gatcaccgcg gctccggccg
     1081 ggcgggacga gagccggacc gacatgccct cctcgatgtt cgcacctggc gagaccagga
     1141 cgatgtcgcc ctcccggagc ggcccctcca ccccgacgac gccccgccgc tcgtcgcgcc
     1201 cgttgacggc gacgggccgg cggacgatct gcccgctctc gaccacgaag acgtgctctt
     1261 cgcccgccgg gccgcgcagc gcggccaccg ggaccagcag cacctcccgc gcggcgcccg
     1321 agagcacccg gccggtcgcg aagagccccc cgaccaggcc gccgggattc gccatggcca
     1381 ggtagacgcc gacctggcgt gtgcccggat cggcggtcgg ctcgatgcgc gcgacctcgc
     1441 cgcggtacgt ctcgccgggc agggcgtcga gggtgaagac gaccccctgc cccacctcga
     1501 ggcggatggc ctcggagacg ggcacctgcc cgttcaattc gagccggctc gtgttgacga
     1561 ccgtgaagag cgtctgattc cggtcgatgg cctcgcccgt ctcgaccagg cggtcgctga
     1621 cgacgccgcc gatgggagac cgcaccgtcg agtgggcggc ctgctcggca gccatcgctt
     1681 cggcggcctg cgcggcagaa agctgggcct gcgccgcctc gtgctgcgcc ttcgcctgct
     1741 cgtactcgat ttccgagagg gcgcccgcct cgtagagcgt ccgcgccgac tcgagctggc
     1801 gcacggcgag cgcgagggcg gccctcgccg ccgccacgcc ggcctgcgcg ctcgcggcct
     1861 ggctaaggat gccctcggcg tcgatcacgg ccagcaccct gccctgcgcg acggcgtcgc
     1921 cccggtcggc gcccagacgc gcgagtgtcc cgccgacctg ggacttcacg tcgacgcgcc
     1981 ggtacggctc cagcgtcccc gtcagggtga tgccctcctc gagcgtgccg cgggtcacgg
     2041 tcgccacgtc gcgctctccg agcacgacgc cctccaggcc ctccgtgccc gccgccgagc
     2101 tgtccgtgtc cgacgcgctg ccgcaccccg cggcgcccgc caggacgaag cccaggagga
     2161 gcgccgccgg aagcctggca agcacgcgga ggcggggcgg gcggagccgg ggcactcgga
     2221 gccgcgccgt acgcgccatg gtgaacccgt tcatgctgat cctgatcatg ggatgaagaa
     2281 gcctctactg caggttgccg cccatggccc ggtccactcc ggcgtcggcc acgtagtagt
     2341 cggtgagggc ccgggccagg ttgacccgcg cctggttcag ggaaaggagc gcgctgaaga
     2401 cgtccagctg cgtcgcgagc ccctgttcat agcgcatctc cgtgagatcg tacacgcgcc
     2461 gggcctggtc gaccgtcgcc tcgcgggcgt cgatctgggc aagcgcgcgc tgcttctcgc
     2521 cgagggcctg ctcgtactcg agctggatgg cctcctcgag ctgccgcacc tgcagctggg
     2581 cctggtgcag ctccacccgg gccccctgca cctgggccgt ccggcggaac ccgtcgaaga
     2641 tcgggatctg gacgttcagg ctcagggtga agtccgtcct ccactgctct ccgaacgcaa
     2701 agagcgtgga cgggtagatg agcttgccgt agctcgtgtt gaaggagacg gtcggcaggt
     2761 accctgaacg ggcgatccgc acctgctgct cgcggatgcg gacctgttcc cgcagggcct
     2821 ggacggacgc ccgctgcgcg ccgagcacct ccggatccag ccggacgtcc acccgcccct
     2881 cggccggtgg cgactcgagc cgcgtgacca gacgcagcgg ctgctcgagc gggacgttga
     2941 cgaggcgctt gaggttcagc tccgcgagct cggccgcgtt ccgcgcctcc acgagctggg
     3001 ggcgcaggtt ctcgagctcg acctgggccc gcatcacgtc gaggtcggag gcccgccccg
     3061 cgtcgaagcg cagccgctcc tcgtccagga agcggcgggc ccgctccagg gcatcgccgg
     3121 agatgcccac cagctcccgc gcgaagaggg cccggtagta ggccgtgcgc acatcgagct
     3181 cgatcgcggc ggtctgttca cggaggttca ggcgcgccgc gtcggccacc gcgctcgcga
     3241 tccgcagccc ggcgcccgtc cgccccccgg aatagaggag ctgcgagccc gagatgccga
     3301 aggtgtacgc gttcttctgt ccgaatggca ggtcgccgaa gagctgggcg agggccccga
     3361 acgccgcatt cggcgtcttg tcctccaggt aggcgatgcg gtcctcgatc gaggccgtcg
     3421 gatcgggctc gaacttgagc gagtccggca gctcgaagtc cccgcccccc gtgtcgaacg
     3481 ccgatttcac cgtgcgcgtg tagcccgccg agccggtgac ctgcggcagg gcggccgacc
     3541 aggcgtcccg tacgcgggcc gccgcgaggt cgacctgcga gcgggcgagc atcacctcct
     3601 cgctggacgc gagcgcgcgc tcgagggcac cgtcgaggct cagatccagg ctgtcggcgc
     3661 gggcggcggg gcgggcagcc tcctgcgtgg cggcggtctg ggccgtggcg gcggcgggca
     3721 tccaaacgcc ggcaacgagc accagcgcgc ggcagagcgt ggatattctc atgactgaca
     3781 tctgcttacg aggtcgcgcg ctccgggtgc tccgcgagcc agcgcgccca ccgctcgggg
     3841 gtgtgctcga agtcgtcgag caggaagctg tagaacccgt gccaggtctc gagcctgcga
     3901 cgggcgtccg cgaggtcctc cgggatctcg tcgaggccct tctcgaccgc ccggagcgtg
     3961 cgccgcatac gctgccggat catttccagc atctgctccc agggcctatc gccgatgcgg
     4021 tagtagtccc ggcggtcgcc cggaacgctc cggcgctcga tgatgccgtg ctgcgcgagc
     4081 atccgggcgt tcgtgctgac ggatcccttg ctcacccgca gccgctcgac gatttcatcg
     4141 agcgaatgcg tgcggccgtc gaccaggagc aggccgatca tgcggccggc gatgcgcgac
     4201 aggccgttcg cctccgaaac gagacccatc tgctcgatga aggcggcgac ggcgtcgtgc
     4261 atggctggaa cccggtcgga ggttcatttc attcaatttg aactgaacga gaggaaacgg
     4321 gctccgtttc gggatggcgt ggccttcacg agagattgac tgaacaggcc gggcgcggcg
     4381 gcccgaccgt gcgggagcgg ccgggcgtgg cgtggcggca atgcggagac ggcggttggg
     4441 cgtagaggac cgactatgca gaagcgcccc gcctcccgaa ggagacgggg cgccccagtc
     4501 atcccgcatt ccgactcgat ctcaggtgag cttgatgtcc ccggcgctcg agcggcggtc
     4561 gctcttcttc gtgggcttcg ggtcggcggg gtcgggatcc gcggggttgg ccttgtgcca
     4621 gcggtcgcct gccctccgac gatccccgac gttgaaggcc agcgcagccg ggctccgaag
     4681 ctcgtcatac gcacgcgtct tttcggcgag gatgtccata gcctcctccg aatgctcccg
     4741 gccgtacgac atgacgacat cctctctcag agacaggccc gcgtgcctgg ccgcgtcgaa
     4801 ggcatcgagg ccggcgccca ggaacacgaa ctgccagccg gcctgctcct tctccttgac
     4861 gagcagcgcg agatcgccgg cgtcgtgttc gacggagcag ttctcgagcc cgtcggtctg
     4921 gatgacgacg accacgtcgg gagagtcgcc ccgggccgcg acggcctcat ctgttgccct
     4981 gatgatcttg accgcggcgt cgatgagcgg cgtcattgcg ttcggccggt agtcctcggt
     5041 cgtgagcggc ccgacgctct ggaccttctc ggcgaccacc cgcgtctgcg tgcggctcga
     5101 gttgaaggtg accaggctga acaggatgtc cgaatcggac ttcgactcct tgagcccctc
     5161 caggtacttg ttgtaggcgc gaatggtgtc gtccttgatc gactgcatcg agccggtctc
     5221 gtcgagcaga agggagacga acagtggctt acgcttcaga ttcatttgta gggtcctcat
     5281 tgtaacgtga acgaatctcc ttgatgagcg aaatgtcgtc gaccagcatc cgcctcacgt
     5341 ccgacgggac cttccccgcc cgcgcgagca cctcgtccga gtccgcctcg agcagccggg
     5401 aaagcgcgac gagcttctcc tcggacaagg acgagtagcc ctccacgtcg ttctcgacct
     5461 tgctcaggta cgtgggctcg atccccaggc gattcgccac gtgccgctgg gtcaggccct
     5521 tctggcggcg gaggcgctgg aggaagtctc cgaaggattc gggcatggcg ggtggcggcg
     5581 cggcgtgctg gtctgtggca ttgtgctttg ttgagggggt tgtacttgtt ggtacaagtg
     5641 caagttcccg ggggtggcgg ctcattcgat gcggcccggt cacgtgcctt cgcggtgccc
     5701 gcgggcgagc ggttcctgtc ggcgcagaag cccgtggggc ggagtgccga gcgggtgggc
     5761 accgctgcta agacctcgca tccggactgg gatcgacacg aggaccttcg ttcgccgtgc
     5821 cccatgtccc ggagccgaat cgccaggcgt acagcatggc gagcccgaag aatatcaggt
     5881 caatcgccga gaaactggcg aagaggccct ccctgcccat cgatgcccgg atggcgggca
     5941 ggcttgcctc gaggtttgcg cgaacctcgg acagtcgggc ctcgccgaag gcctcctgct
     6001 cgtcctcggt catctccgac catctttgaa ccgtctccgc ccatacgtcc gacgggtagt
     6061 ccgtggccgc cgaagcagtc gacgggtcca ccccgacggg ccagtccacc tgtcggccgg
     6121 catccacgta ttcctccacg accgcatccg cgatgaacga catcacgaat tcctcgtcct
     6181 ggtaggactc tacgtacatc gcgacgattt cgtcgtcgtc cggaatgaac agatcgacca
     6241 tggcgatctt tcccgcgaca atcgacagca tggcgaggat gactgccatc acgcccaagg
     6301 ccgttggcga ccgccgggat tccctgctgc cggccgccac ggccaggccg acgagggcgc
     6361 cgacagcgat ggcgacgtac ccgacctcat agtcggttgc gtacgctatc ccagcccagg
     6421 ccagagcgcc gactgcgccg gccgccgtcc cgtaaatgaa gctcttcatg acttcctgga
     6481 gcggttggtg aaaggtcgga ggactcgcgc gccacggtgc gagcgtcgcg gagcgtcgaa
     6541 tccgccacgc ggccacggcc tgcagcgccg ctattgccgg gtgcatgcta gaagcagcga
     6601 taccggccaa accggacata agggcgtgac gcttcgggca cggcccgccg gggaagatcg
     6661 agccgggatg cgtcccccag cggtccgtaa ccccgctcac ctcacccgca tcaccatccg
     6721 ggtgctcacc gccccacccg actcgagccg caccaggtac gcccccgggg gcagcgccgc
     6781 cgcgtcgagc tcggccgcgt gcgccccggg cggcagctgg gcgtccacga gccgcgccac
     6841 cgtgcgtccc aggaggtcga cgacctcgag ccggacgctg ccggccttct ccaggcggaa
     6901 cgggatcgtc gtgcggtcgc cgaacggatt ggggaaggcc ggttcgagca ccggcgcacc
     6961 cacatgcgga atcggctcgg tagcgacccc gccggctgtc gaccatcgcc ggccgaagat
     7021 gtcgccgtcc tcccagacga ggagcacctc gtcccggagc gcacgatggg ccgcgtccgg
     7081 ggcctgcgcc tggcctatgc cggagagcgg catggcgggc ccgggccccg gcagcgccgg
     7141 atcgagggca cgtgccgcga cctggaaggc gcccggaccg aacgaggcgc gcca
//
LOCUS       DBNHQZ010000345         7534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3081602, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000345 DBNHQZ010000000
VERSION     DBNHQZ010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3081602"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="AC16YU_09250"
     CDS             <1..1302
                     /locus_tag="AC16YU_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065452.1"
                     /translation="AWTGDRASAVFEVERRMDEWPTGTRLEAASSSWTGTARARFAPS
                     GEWSTSGEVSWRRRTFTDAFAGRADARDTRSLSLSLTGDGRPASRIRLGWMYQARSER
                     SPTLQEVYFRAGPDRGEYVWIDDNGDGVIQVDEFVPETTPDEGLYVRTWLPSDSLASV
                     TVAQARIRLALEPGHGTPGLAGRVSGQTLVEVEERSRTGRRSDVYLLRLDRFRVPGTT
                     LNGRLRFRQDVSLFRGSPAFGIDASWDVSRALSELAAGRESRRLDRRQVAVRGRPHAS
                     LDLAVDLVHERNATDSDAFASRRFDLLTRAVAPSIRYRLGRATTARAELSLAWKRDRR
                     VERSATVVRLPVVLRTGLSDVVDLTVRLEAADARVRGAVAGLAAWEMTEGRGAGRSLL
                     WGTTLQWAASRVLRATVAYDGRAPATGAAIHTGRVELSAIF"
     gene            complement(1306..2610)
                     /gene="murA"
                     /locus_tag="AC16YU_09255"
     CDS             complement(1306..2610)
                     /gene="murA"
                     /locus_tag="AC16YU_09255"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6065453.1"
                     /translation="MDKLVIRGGQPLYGSLEVGGSKNTALALMSGALLADGVTTIDRV
                     PALRDVHTFSRVLDVTGAVTRFDAAGHRLVVDASAVDKPVAPYELVKRMRASFYMLGA
                     LLGRSGRAKVSLPGGCAWGPRPVDLHIEGLKAFGAEIDLEAGYVVANAPGGRLAGGRF
                     TLTPSSVGATVNLLLGAVTARGGSRIGNAAIEPDVVEFSRMLEAMGARIHGIGTRTLE
                     IEGVEALHPVEWINCPDRIEAGTYMIAAAMAGRPGVPITVKGVNTEHLGEVFLDAFAR
                     TGTRITAEGESVHVVPPETLEAVSIETAVFPGFPTDLQAQWTVLLACSAGNAKVHDTV
                     YTDRFKHIPELRRMNVNAIVVGGEVVVEGGRGIQGAHVMSTDLRASVSLVLAGLVAEG
                     ETHVLRVYHLDRGYEDLEGKLARAGADIRRESYDEWADPSVE"
     gene            2714..3862
                     /locus_tag="AC16YU_09260"
     CDS             2714..3862
                     /locus_tag="AC16YU_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR6065454.1"
                     /translation="MDPVYLDHAATTPLDPGVLRVMLPYLSERFGNASSVHARGRVAR
                     HAVEQARSAIAATMDLEPGQVWFTSGGTEANNTAIRGVAARRRGAVVTGAAEHESVLR
                     CVERLAGEGRETVLLPPGSDGSVDPDAVLGALSGEVALVSLMRANNEVGTLTAVREIA
                     AACRERGVPMHADAVQSCAYEGIAPGDLGVDLVTVSAHKIGGPSGVGALLVAPGVEVE
                     PLIVGGGQERGRRAGTENVAAIVGFAEALRLVAESREREAARIAALRDRLARSLAEEL
                     GDRVSITTPLDRSAPHILHCTFPEAGGRRTDGEMLLLGLDLAGVQASAGSACSSGAVE
                     PSHVLLSMGMTRSRAASALRLSLGRTTTADEIDRAVPAIVGTVRQVTA"
     gene            3859..4713
                     /locus_tag="AC16YU_09265"
     CDS             3859..4713
                     /locus_tag="AC16YU_09265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6065455.1"
                     /translation="MTQATKVRVADRIERHAAFGTGRVVPRNVDVRLPAGYDLDPEQR
                     YPVVYMHDGQNLFDPETSFSGVAWGVDRTLDRLSREEGIAEAIVVGIWNSPRRISEYM
                     PQRAMEEFGTAMMRRRFAETYGGAPCSDDYLAFLVHELKPYIDARYRTRPEAEATLVM
                     GSSMGGLVSVYALCEHPSVFGGAGCLSTSWTVAGRIMLQYLQRRLPAAGGHRLYFDYG
                     VEAQIGRYESLQKAVDRIVHRAGYRRGRDWLSERFPGEPHSEAAWRDRFDVPARFFFG
                     QDQPRPSG"
     gene            4811..5863
                     /gene="fbp"
                     /locus_tag="AC16YU_09270"
     CDS             4811..5863
                     /gene="fbp"
                     /locus_tag="AC16YU_09270"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR6065456.1"
                     /translation="MPSTAARQTRDDPGSILTLEQFIIDRQEQFPHSTGAFSRLIRDL
                     SVASKVVNFHMRRAGLIDVLGASGLVNVQGEVQQKMDALAHEEFVRALRRGGECCLIG
                     SEEHAEAIPLRTDDSPEGKYIVLMDPLDGSSNIEVNISVGTVFSIYRLADGETEVTLE
                     AALQPGVQQVGAGYVVYGSSTMLVYSTGFGVNGFTLDPSLGEFLLSHPDIRIPRDGRM
                     YSINEGNYESFEPGLKRYIKWMQREDPATGRPYSTRYIGSFVADFHRNLLKGGLYIYP
                     ATEASPEGKLRLMYEANPMAYIVEQAGGMASDGAARILEKRPRALHQRTPLYIGSRAM
                     VERAEAFLEGADDVTA"
     gene            5860..6819
                     /locus_tag="AC16YU_09275"
     CDS             5860..6819
                     /locus_tag="AC16YU_09275"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - L-threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR6065457.1"
                     /translation="MTADSNRVRAWGPASLSNLGPGFDALGMCLAGLGDTVEVWQVPE
                     PGIRITSEPPTDSVPTDPEYNTAAAAARVVLNRFAPGTGLEMRIHKGILPGSGLGSSA
                     ASAVAGAWATSVLLGGPPNRASVVGAVLEGESLVSGAPHGDNVLPSLFGGLVLVSSGD
                     PLRWRRLPVGGNVALAIVRPDITILTREARAMLPKKVLLSDAVRNAASLAFLVDAFRA
                     GDMETVGRCIEEDRLVEPVRARLVPSFGAIRRAAKEAGALGCALTGSGPAMFAICSDD
                     TSARRVAQAMHEAAGEGDVRSIVTHVDPKGARITDVARPGVVL"
     gene            6840..>7534
                     /locus_tag="AC16YU_09280"
     CDS             6840..>7534
                     /locus_tag="AC16YU_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6065458.1"
                     /translation="MSTRNPDGRPVGFVEAMLRGLAPDGGLYVPTRIPAGDGFAGPDF
                     ADHSAAFLLPWLRAEFGAGEAEKAVRSALDFPVRIVEPSVGTFVVELFHGPTLSFKDF
                     GARTMARLVQAAFRDAPTGRTIFVATSGDTGSAVADGFSGMSGVRVVLLYPEGQVSPV
                     QERQIAAPRPGVLAVRVQGSFDDCQRMVKAVFADPRFDRFAPTSANSINVGRLLPQAT
                     YHAWASSRVPDAVL"
BASE COUNT         1058 a         2584 c         2789 g         1103 t
ORIGIN      
        1 gcgtggacgg gagaccgggc ctcggcggtc ttcgaggtcg agcgtcgcat ggacgagtgg
       61 ccaacgggca cccggctcga ggcggcgtct tcgtcgtgga ccggcaccgc gagggcgcgc
      121 ttcgccccgt cgggagaatg gtcgacgtcc ggagaggtct cgtggcgccg ccggacgttc
      181 acggacgcgt ttgccgggag ggcggacgcc cgggacacgc gcagcctctc gctctcgctc
      241 accggggacg gacggccggc ttcgcggatc cgcctcggct ggatgtacca ggcgcgctcg
      301 gagcgttcgc ccacgctgca ggaggtgtat ttccgcgccg gccccgatcg cggcgagtac
      361 gtctggatcg acgacaacgg ggacggcgtc atccaggtcg acgagttcgt accggagacg
      421 acccccgacg agggactcta cgtccggacc tggcttccgt cggactcgct cgcgtcggtg
      481 acggtggcgc aggcgaggat ccggctggcc ctcgagccgg ggcacggaac gccgggcctg
      541 gcgggccggg tgtccgggca gaccctggta gaggtcgagg agcgcagccg gaccggacgg
      601 cggagcgacg tgtacctgct caggctggac cgattccggg tgcctggcac gacgctgaac
      661 ggccgcttgc gcttccgcca ggacgtgtcg ctcttccgcg ggtcacccgc cttcggcatc
      721 gatgcctcgt gggatgtgag ccgggcgctc tcggagctcg ccgccggccg ggagtcgcgg
      781 cggctggatc gccggcaggt cgcagtccgt ggcaggcccc acgcgagcct cgacctggcc
      841 gtggacctgg tgcacgaacg gaatgccacc gattcggacg cgttcgcttc ccggcggttc
      901 gaccttctga cgcgggccgt cgcgccgtcg atccggtatc gcctgggccg ggccacgacc
      961 gccagggccg agctgtcgct cgcctggaag cgggaccggc gggtggagcg gtcggcgacg
     1021 gtcgtccggc tgcccgtagt gctccgcacc gggctgagcg atgtcgtgga cctgaccgtg
     1081 cgcctcgagg cggccgacgc ccgggtccgc ggggccgtag ccgggctcgc ggcctgggag
     1141 atgaccgagg ggcggggcgc cgggcgttcg ctgctctggg gaacgacgct ccagtgggca
     1201 gcgagccgcg tactgcgcgc cacggtggcc tacgacggcc gggcgccggc gacgggggcg
     1261 gcgatccaca ccggccgggt cgagctcagc gcgatcttct gagcgctact cgacggacgg
     1321 atccgcccac tcgtcgtagc tctcgcgccg aatgtcggct cccgcgcgcg cgagcttgcc
     1381 ctcgaggtcc tcgtaaccgc ggtccaggtg gtagacgcgc agcacgtgcg tctcgccctc
     1441 ggccacgaga ccggcgagaa cgagcgagac ggaggcgcgc aggtccgtgg acatgacgtg
     1501 ggccccctgg atgccccggc caccctcgac gaccacctca ccgcccacca cgatcgcgtt
     1561 gacgttcatg cggcgcaact cggggatgtg cttgaagcgg tccgtgtaca ccgtgtcgtg
     1621 gaccttggcg ttgcccgccg agcaggcgag cagcacggtc cactgggcct ggaggtcggt
     1681 cgggaaaccg gggaagaccg cggtctcgat ggagacggct tcgagcgtct cgggagggac
     1741 gacgtggacg gactcgccct cggccgtaat ccgcgtgccg gtccgggcga aggcgtccag
     1801 gaagacctct ccgaggtgct ccgtgttcac ccccttcacc gtgatcggca cgccgggccg
     1861 gccggccatc gcggccgcga tcatgtacgt gccggcctcg atgcggtccg ggcaattgat
     1921 ccattcgacc ggatgcaggg cttcgacacc ctcgatctcg agtgtccggg tgccgatgcc
     1981 gtggatccgg gcccccatcg cctcgagcat ccgcgagaat tcgacgacgt cgggctcgat
     2041 ggcggcattg ccgatccgcg agcctccacg ggccgtcact gcgccgagga gcaggttcac
     2101 ggtcgcaccc acgctcgacg gcgtgagggt gaaacgtccg cccgccagcc ggccccccgg
     2161 cgcgttcgcg acgacgtagc ccgcttcgag atcgatctcg gcgccgaacg ccttcagtcc
     2221 ctcgatgtgg aggtccacgg ggcgcggccc ccacgcgcag ccaccgggaa gggacacctt
     2281 cgcgcgcccc gaccggccga gcagcgcacc cagcatgtag aaggacgccc gcatgcgctt
     2341 caccagctcg tacggggcca cgggcttgtc cacggccgac gcgtcgacga cgagccgatg
     2401 gccggcggcg tcgaacctgg tcacggcccc ggtcacgtcc agtacccgcg agaacgtgtg
     2461 tacgtcgcgc agcgccggca cgcgatcgat cgtggtgacg ccgtccgcga gcagcgcgcc
     2521 ggacatgagc gcgagtgccg tgttcttgga gccgcccacc tcgagcgacc cgtacagcgg
     2581 ctgaccgccg cgaatgacca gcttgtccat gcagggagtg ggttcagcgg cccgatgata
     2641 cgggcacggt acccgaaaag gaaggatccg tcaagatcgg acggcgtagg gaaagccccc
     2701 tgacccgtgc ccgatggatc ccgtctacct ggaccacgcc gcaaccacgc ccctggatcc
     2761 gggcgtgctc cgcgtcatgc tcccgtacct gtcggaacgg ttcgggaacg cgtcgtcggt
     2821 ccacgcccgc gggcgcgtgg ccaggcacgc ggtggagcag gccaggagcg cgatcgcggc
     2881 gacgatggac ctggaacccg gacaggtctg gttcacgagc gggggcaccg aggccaacaa
     2941 cacggcgatt cgcggcgttg ccgcccgtcg gcgcggcgcc gtcgtcacgg gcgcggccga
     3001 acacgagtcg gtgctgcgct gcgtggagcg cctggcgggg gaaggccggg agaccgtgct
     3061 cctgccgccc ggttccgacg gctcggtgga cccggatgcg gtgctcggtg cgctctccgg
     3121 cgaggtggcc ctggtctcgc tcatgcgggc caacaacgag gtcggaacgc tcaccgccgt
     3181 ccgcgagatt gccgcggcgt gccgtgagcg gggcgtgccg atgcatgcgg atgccgtgca
     3241 gtcgtgcgcc tacgagggca ttgcgcccgg ggacctggga gtggacctcg tgacggtgtc
     3301 ggcgcacaag atcggcggcc cgtcgggcgt cggcgccctg ctcgtcgcgc cgggcgtgga
     3361 ggtcgagccg ctcatcgtcg gaggcggcca ggaacgggga cgccgcgccg ggaccgaaaa
     3421 cgtggcggcc atcgtgggtt tcgccgaggc gctgcgcctg gtggcggagt cgagggaacg
     3481 ggaggcggcg cggatcgcgg cgctccgtga ccggctggcg cgcagcctcg cggaggagct
     3541 tggcgaccgg gtctcgatca cgacgccgct cgaccggtcg gcgccccaca tcctgcactg
     3601 caccttcccc gaggccgggg gccggcggac ggacggggag atgctgctcc tcgggctcga
     3661 cctggcgggc gtgcaggcat cggccggctc ggcctgctcg agcggggccg tcgagccgag
     3721 ccacgtgctg ctctcgatgg gcatgacgcg aagccgcgcg gcctccgcgc tccgcctgtc
     3781 cctgggacgc accacgacgg cggacgagat cgaccgcgcc gtccccgcga tcgtcggcac
     3841 ggtccggcag gtgacggcgt gacccaggcc accaaggtcc gcgtggcgga tcgcatcgag
     3901 cggcacgcgg ccttcggtac cgggcgcgtg gtgccgagga acgtggacgt ccgcctgccg
     3961 gccgggtacg acctggaccc ggaacagcgc tatccggtcg tctacatgca tgacgggcag
     4021 aacctgttcg accccgagac atcgttcagc ggggtggcct ggggggtgga caggaccctg
     4081 gaccggctga gccgcgagga gggcatcgcc gaggccatcg tggtcggcat ctggaactcg
     4141 ccccggcgga tttcggaata catgccgcag cgtgcgatgg aggagttcgg cacggccatg
     4201 atgcggaggc gtttcgccga gacgtacggg ggcgcgccgt gctcggacga ctacctcgcg
     4261 ttcctggtgc acgagctgaa gccgtacatc gacgcccgct accggaccag gccggaggcc
     4321 gaggcgacgc tcgtcatggg ctcgagcatg ggcggactcg tgtcggtgta tgcgctgtgc
     4381 gagcatccgt ccgtgttcgg cggggcgggc tgcctgtcca cgagctggac cgtggcgggc
     4441 aggatcatgc tccagtacct gcagcggcgg ctccccgccg cgggcgggca ccgcctgtac
     4501 ttcgactacg gagtcgaggc gcagatcggg cggtacgaat ccctccagaa ggcggtcgac
     4561 cgcatcgtgc acagggcggg ataccggcgg ggccgcgact ggctgagcga gcgcttcccc
     4621 ggggagcccc actcggaggc ggcctggcgc gaccggttcg acgtgcccgc ccgcttcttc
     4681 ttcggccagg accagccgcg accgtccgga tgaaccgctc ggacccggaa cgaacgggca
     4741 gcggacccgc gccgcgttgg accgatgtgc gggccggtct attttccggc ccccacccgt
     4801 acccatcccg atgccctcga cggccgccag acagacgcgt gacgaccccg gctcgatcct
     4861 gacgctcgag cagttcatca tcgaccgcca ggagcagttc ccgcactcga cgggtgcctt
     4921 ctcccggctg atccgggatc tgagtgtcgc gtcgaaggtc gtgaacttcc acatgcgccg
     4981 ggccggtctc atcgacgtgc tcggagcctc cgggctggtc aacgtgcagg gcgaggtgca
     5041 gcagaagatg gacgctctcg cccacgagga attcgtgcgc gcgctgcgcc ggggcgggga
     5101 gtgctgcctg atcggctcgg aggagcacgc cgaggccatt ccgctccgca cggacgactc
     5161 gcccgagggc aagtacatcg tcctgatgga cccgctcgac ggatcgtcga acatcgaggt
     5221 gaacatctcc gtcggcacgg tcttctcgat ctaccggctc gccgacgggg agaccgaggt
     5281 cacgctcgag gccgcgctcc agccgggcgt ccagcaggtg ggagccgggt acgtggtgta
     5341 cgggtcgtcg acgatgctcg tgtactcgac cggcttcggg gtcaacggct tcacgctgga
     5401 cccgtcgctg ggcgaattcc tgctctcgca ccccgacatc cgcatcccgc gggacgggcg
     5461 gatgtactcg atcaacgagg gcaattacga gtccttcgag ccgggcctga agcggtacat
     5521 caagtggatg cagcgcgagg accccgcgac gggacgaccc tacagcacgc gctacatcgg
     5581 ttccttcgtg gccgacttcc accggaacct gctcaagggc gggctctaca tctatccggc
     5641 gacggaggcc agccccgagg gcaagctgcg cctcatgtac gaggcgaatc ccatggccta
     5701 catcgtggag caggcaggcg gcatggcctc ggacggcgcg gccaggattc tcgagaagcg
     5761 tccgcgcgcg ctccaccaga ggacgccgct ctatatcggg agccgggcga tggtggagcg
     5821 ggccgaggcc tttctcgagg gcgcagacga cgtgaccgca tgaccgccga ttccaatcga
     5881 gtgcgcgcct gggggccggc ttcgctctcg aacctgggcc ctggtttcga cgcgctgggc
     5941 atgtgcctgg cgggcctggg cgatacggtc gaggtctggc aggtcccgga gcctggaatc
     6001 cggatcacgt ccgagccgcc gaccgattcc gtgccgacgg atcccgagta caacacggcc
     6061 gccgccgcgg cccgcgtggt gctgaaccgt ttcgcgcccg gcaccggcct ggaaatgcgg
     6121 atccacaagg gaatcctgcc cggctcgggc ctgggcagct ccgcggccag cgcggtcgcc
     6181 ggggcctggg ccacaagcgt gctgctcggc ggaccgccca accgcgcgag cgtcgtcggt
     6241 gccgtgctcg agggagagag cctggtgtcg ggcgccccgc acggcgacaa cgtgcttccc
     6301 tcgctgttcg ggggactggt gctggtttcg tccggcgatc cgctccggtg gcgccgcctg
     6361 ccggtcgggg gcaacgtggc gctcgccatc gtccgccccg acatcacgat cctcacgcgc
     6421 gaggcccggg cgatgctgcc gaagaaggtg ctgctttccg acgccgtgcg caacgctgcc
     6481 tccctggcct tcctggtcga cgccttccgg gccggggaca tggaaacggt cgggcggtgc
     6541 atcgaggagg accggctcgt ggagcccgtt cgggcgcggc tcgtgccctc gttcggggcg
     6601 atccgccggg cggctaagga ggccggtgcc ctcgggtgcg cgctcacggg ctcgggaccc
     6661 gccatgttcg cgatctgcag cgacgacacg tcggcccggc gggtcgcgca ggcgatgcac
     6721 gaggcggcgg gcgaggggga cgtccggtcc atcgtgaccc acgtcgatcc gaaaggtgcc
     6781 cggatcaccg acgtggcgcg gccgggggtg gtcctgtgac cggagggccg atcgggtggg
     6841 tgagcaccag gaatccggat ggccgtcccg tcggattcgt cgaggcgatg ctgcgcggcc
     6901 tcgcgccgga cggcgggttg tacgtgccga cgcggatacc cgcaggcgac ggcttcgcgg
     6961 gccctgactt cgccgaccac tcggcggcat tcctcctgcc atggctgcgg gcggaattcg
     7021 gagcagggga ggccgagaag gcggtccggt ctgcgctgga ttttccggtc cgcatcgtcg
     7081 agccgtccgt cggcacgttc gtcgtcgagc tcttccacgg ccccactctc tccttcaagg
     7141 acttcggggc gcgcaccatg gcccgcctgg tgcaggcggc gttccgggac gcgccaaccg
     7201 ggaggacgat cttcgtggcg acctccgggg ataccggcag cgcggtggcg gatggattct
     7261 ccgggatgtc cggcgtccgg gtggtcctcc tgtacccgga gggccaggta agccccgtgc
     7321 aggaacgcca gatcgcggcg ccccggccgg gggtcctcgc ggtgcgggtt caggggtcat
     7381 tcgacgactg ccagcggatg gtcaaggcgg tgttcgccga cccgcggttc gaccggtttg
     7441 cgccgaccag cgccaactcg atcaacgtgg gccggctgct gccgcaggcc acgtaccacg
     7501 catgggcgtc ctcccgggtg ccggacgcgg tgct
//
LOCUS       DBNHQZ010000346        10843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3761826, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000346 DBNHQZ010000000
VERSION     DBNHQZ010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3761826"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="AC16YU_09285"
     CDS             complement(<1..549)
                     /locus_tag="AC16YU_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6065459.1"
                     /translation="MVHRRFRLQVLVRAALLAATLGAGAWLAYAGGLVVPAILALLFA
                     AWQVMLLVRYVEKTNRDLARLLRSIRYSDFSQTFGSDGRGGGFTELAGAFRDVMDDFR
                     AARAEKEEGYRYLETVMQHVGVGLLSFDASGEVSLVNGAAKRLLRVKHLRTVQSLRTT
                     SPELVDALLTLPYGGKALVRVTA"
     gene            complement(587..1975)
                     /locus_tag="AC16YU_09290"
     CDS             complement(587..1975)
                     /locus_tag="AC16YU_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6065460.1"
                     /translation="MADLLGRVLIVDDDEDVLQALRLLLKKHARRVQTEKDPERIPAL
                     VAGEDFDLILLDMNFREDVTSGREGFHWLERILEVDPSAVVVLVTAFGDVQTAVEAIK
                     AGATDFVLKPWENERLLATLSTATRLRHARRENERLRSRGRQLSDDLDHRFQDVVGGA
                     AASMQRVFETIDKVAATDANVLILGENGTGKELAARAVHRASPRAGEVFVTVDMAAIP
                     ETLFESELFGHVRGAFTDAREDRAGRFEIASGGTLFLDEIGNLSLPLQAKLLTALQRR
                     EVYRVGSHRAIPIDVRLICATNMPVYEMVRDGRFRQDLLYRINTVEVRLPRLAERQED
                     LPLLVDHFLERYARRYGKAVRGLTPAAMARLARYHWPGNVRELQHMIERAVIMTETPM
                     LQPEDFFFAGHHEGATRRDGFVFDTYNLEEVEKTVIRQSLDACHGNISRAAEQLGLTR
                     TSLYRRLEKYGL"
     gene            complement(2050..4449)
                     /locus_tag="AC16YU_09295"
     CDS             complement(2050..4449)
                     /locus_tag="AC16YU_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065461.1"
                     /translation="MLRSYLKTAFRALRRDGFYSALNVLGLAVGLALFFLIVSWIRFE
                     RSYDAFHDRADRTYRVVKRDAGNEFMGTDRFAVTPAPLETALLDEVPGVEATTRIRRV
                     EALLRAGERTDVSRGLLATGTFLDVFSFRLLRGDAAGALSRPGAVLLTPALAARLFGD
                     EDPLGETIEYEFFGDSYSLEVTGIVGPPPDNSHIGYAYVVSASTEPDFEESLDDWGSS
                     SWHTYAVLAPGTDAEAFARRIDGLADAHLRGESWYRGGPDRKTTLQAQPIRDIHLQSR
                     INFELKANGSATQVAWGLALALLVLLIPCTNYMNLAAARAATRRREVGVRKAIGADRR
                     QLAAQFLGESVLVAAISALAAAGIVAMARPAFSALVLRDIPPSFLLDPPAIALALAAV
                     LVVGVLSGSYPALVLSRPRAAAVLRGDPVLGSRRSRLRDVLVVAQFAIGIGLIFGTMV
                     VHGQIGFAGSTDTGMERDQVLAIRVRDSSVRERWAALTNEMRAVPGVGAVSGASHLPT
                     DIQQSNGTREWDGREEGQEMRVYLARVDYGFVEMLGLDLVRGRSFSPGFAADSDRGLI
                     LNETAARAFGWEDPVGRHVGFGDTPMEVVGVVRDFHFHSFRQEIGPLALSLRPDGLSY
                     VLLRIDGRDTGAVLAAVEQVWKRFSNGFPFDAQFLDDAFDRHFEEDQRLADMLDAFAA
                     VALVIACLGLFGLAAFVAQQRTKEIGVRKVLGAGARDIVFLLSRDFTALVVVAVLVGC
                     PVSFVLMDRWLDGFAYRITPGLGTALAAASLAVGVGWLTVAWQALRAANLDPVRSLRY
                     E"
     gene            4704..6047
                     /locus_tag="AC16YU_09300"
     CDS             4704..6047
                     /locus_tag="AC16YU_09300"
                     /inference="COORDINATES: protein motif:HMM:NF052760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6065462.1"
                     /translation="MAETTPATPPGRPSILTTPIGSGSGKATGEGVDRRLAKRFWTGR
                     RIAMVAGGFVVLALVVWAFTQSLGGRRLRVQAERLTVSTVEIGPFQEYVPVTGSVMPR
                     TTVYLDAVEGGRVEEIFVLEGATVEQGTPLLRLSNNDLQLRLINADAQRLEQINRVQD
                     MQFRMEQNALDLRQQLAQMDYQIQRLERLNTRNEELLAIQGISRQEYDSVRDELDYWT
                     RNRTLTLEGYRQDSLRMSGQLMQMQESVRRMEANYDVIESILGNLTVRAPVAGQVTAL
                     NVELGELHTPGSQFGQIDMLDGFKVRAGVDEFYIARVHRDQPATTLPIGDTEYPMVVT
                     RVYPQVTDGRFQVDLEFEGEPPPGIRRGQSVRFRLEMSEPAEALLLPMGGFFQATGGN
                     WIYVLDGSGQAVRREIRVGRRNPQYLEVVEGLGPGDRVVTSGYDTFGDAERLILE"
     gene            6086..6781
                     /locus_tag="AC16YU_09305"
     CDS             6086..6781
                     /locus_tag="AC16YU_09305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065463.1"
                     /translation="MIQTANLTKAYRTDEVETVALADVTIDIAPGDFVSVMGPSGCGK
                     STLLNILGMLDNPTSGSYRFDGTEVANLSERQRARLRKGNIGFVFQSFNLIDELSVFE
                     NVELPLLYLGVSADERKARVQAALERTAIAHRQDHFPQQLSGGQQQRVAIARAVVANP
                     GLILADEPTGNLDSRNGEEVMKLLVELNEAGTTIVMVTHSPADAEYSHRTIHLFDGHV
                     VTENFMASHVPGV"
     gene            6828..9242
                     /locus_tag="AC16YU_09310"
     CDS             6828..9242
                     /locus_tag="AC16YU_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065464.1"
                     /translation="MIRHYLTVSFRHLARQKAYALINVFGLAVGIAACWLIFLYVQDE
                     LTHDAFHERADRIFRVTEVAYTPDGAIESQSAYLPYPMKPTLENEIPEVEEALRTDPV
                     TVLLRVGDRTFEQEILCADPELFTVFSFPLAAGDPAYALADRNSVVLTENMAANLFPD
                     GDAVGRTVEVRLRDRFEPLTVTAVARDVPGGTSIPFQVVVPFEKLAEASGWIGTRDDR
                     WNSYSFYTWVLLREGATEAAFEAKMPALLEKYRPGIRERFIEQGRWEGEGHPRGFLLQ
                     PLGSVHLDPSIAGGIVPPSDPRYAWILGGIALALLLIACINFMTLAIGRSAGRAREVG
                     VRKASGARRRQLMAQFWGESLVMAALALVLGLALASVALPTFNELAGKSLALGSGSWG
                     LSIGVLAGLCLVVGLVAGSYPALVLSSFRPADTLYRTPRLGGSNVLTRSLVVVQFALA
                     VGLIAGTGVMMRQVRFLQERNLGFDRENLVVVDHQGLEGRPLVAHLRRELESRADVVG
                     VTGASMAFTHGSDRHGWVHEGKEYRSFVYYVQEDYLDVMGIPLLEGRFFDPARPADST
                     ATVVVNEAFVRMWGWTDPVGRRVPGYEGEPVVIGVVADVHNQSLHEEIAPIMLSRSPD
                     SWYGNTLVRVAPGGVAGALAAIEAAWLRASPELPVKTSFLDEDLDRQYASERRWSRIV
                     GWSGALAVLVACLGLFGLAALTVARRTKEIGIRKVLGASGTRVAARLSAEFARPVLVA
                     SALAIPVAWILLQRWLEGYAYAVSLPGAVIVGSAAIVLALAVATVGLQSIRAARANPV
                     HALRYE"
     gene            9252..9968
                     /locus_tag="AC16YU_09315"
     CDS             9252..9968
                     /locus_tag="AC16YU_09315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065465.1"
                     /translation="MNEPLLRIVNVTKSYHRGLQASHVLRRVSFDVARGDFVTIMGPS
                     GSGKSTLLHILGLLDEPSEGEYWFDGQAAHAIGERRRSELHRGHIGFVFQAYHLIDDL
                     TVYENLETPLIYRGLSKADRRGRVAETLDRFNMVAKKDLFPSELSGGQQQLVGVARAL
                     IIEPSVILADEPTGNLHSSQGDEIMEQLRRLNRDQGVTIVQVTHNAAYAGYGHRIIEL
                     LDGRIRDDRPVGAVHSATVP"
     gene            9987..10262
                     /locus_tag="AC16YU_09320"
     CDS             9987..10262
                     /locus_tag="AC16YU_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065466.1"
                     /translation="MSVICIPIPDLQRHRTVGLEISVDGVRRVMNYRVETVPWPPDLD
                     PEARIEQLRSYLRMHDPEWELVQIGPPDGDRVPVTFRQHVGIATAPA"
     gene            10303..>10843
                     /locus_tag="AC16YU_09325"
     CDS             10303..>10843
                     /locus_tag="AC16YU_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065467.1"
                     /translation="MLRNYLTVAFRRFRRGGTHVAVNVLGLALGTATCVLLFLVVYTE
                     WSYDRFHENADSIYRTYFWYEDPDGKPGIQTMMPPDFTPEFKATFPAVVEATRLVQSE
                     RDYRQDETTRRFMLSEVDPDFFRMFSFPAVAGDPVATIADPRGMVITTDVARAAFGGV
                     APDWAAILGRTIVIPLDTTE"
BASE COUNT         1672 a         3819 c         3756 g         1596 t
ORIGIN      
        1 cgcggtcacc cggacgagcg cctttccgcc ataggggagc gtcaggagcg cgtccacgag
       61 ctccgggctc gtcgtgcgaa ggctctggac cgtgcgcagg tgcttcaccc ggagcagccg
      121 cttggcggcg ccgttcacga ggctgacctc gcccgatgcg tcgaacgaca ggagtccgac
      181 gcccacgtgc tgcatgacgg tctccaggta gcggtagccc tcttccttct ccgcccgggc
      241 ggcccggaag tcgtccatca cgtcgcggaa cgccccggcg agctccgtga aaccgcctcc
      301 ccgcccgtcc gacccgaagg tctgcgagaa gtcggaatac cggatcgagc gcaggagccg
      361 cgccaggtcg cggttggtct tctcgacgta gcgcacgagg agcatcacct gccaggcggc
      421 gaagagcagg gcgaggatcg ccggcacgac caggcccccg gcgtacgcga gccacgcccc
      481 cgccccgagc gtcgccgcga gcagcgccgc gcggaccagc acctggaggc ggaatcggcg
      541 atgcaccatg gggaggctcc gtcggggcag gccgcgaggc cggggatcag aggccgtact
      601 tctcgagccg gcggtagagc gacgtccgcg tcaggcccag ctgctccgcc gcgcggctta
      661 tgttgccgtg gcacgcgtcg agcgactgcc ggatcacggt cttctcgacc tcctcgaggt
      721 tgtacgtgtc gaagacgaag ccgtcgcggc gggtggcgcc ctcgtggtgc cccgcgaaga
      781 agaagtcctc gggctgaagc atcggcgtct ccgtcatgat gacggcgcgc tcgatcatgt
      841 gctggagctc gcgcacgttg ccaggccagt ggtaccgggc gaggcgggcc atggccgcgg
      901 gcgttaggcc gcggaccgcc ttcccgtacc ggcgtgcgta tcgttccagg aaatggtcca
      961 ccaggagggg caggtcctcc tgccgctcgg cgagcctcgg taggcgcacc tcgacggtgt
     1021 tgatccggta gagcaggtcc tgccggaacc gcccgtcccg caccatctcg tacacgggca
     1081 tgttggtcgc gcagatcagg cgcacgtcga tcgggatcgc gcggtgcgat ccgacccggt
     1141 agacctcgcg gcgctggagc gccgtcagca gcttggcctg gagcggaagc gacaggttgc
     1201 cgatctcgtc gaggaacagc gtcccgccgg aggcgatctc gaaccgcccg gcccggtcct
     1261 cgcgggcgtc cgtgaaggcg ccccggacgt gcccgaagag ctcgctctca aagagcgtct
     1321 ccggaatggc cgccatgtcg accgtcacga agacctcgcc ggcccggggg gaggcccggt
     1381 gcactgccct ggccgccagt tccttcccgg tcccgttctc gccgaggatc agcacgttcg
     1441 cgtcggtggc ggccaccttg tcgatggtct cgaagacgcg ctgcatggac gcggccgcgc
     1501 cgcccacgac gtcctggaac cggtgatcga ggtcgtcgct cagctgccgc ccgcgcgagc
     1561 gcagccgctc gttctcgcgc cgcgcatggc gcaggcgcgt ggccgtcgag agcgtggcca
     1621 gcaggcgctc gttctcccac ggcttgagga cgaagtccgt cgcgccggcc ttgatggcct
     1681 ctacggcggt ctgcacgtcg ccgaaggccg tgacgagcac gacgacggcc gacgggtcga
     1741 cctcgaggat ccgttcgagc cagtggaagc cctcgcgccc gctcgtcacg tcctcgcgga
     1801 agttcatgtc caggaggatc aggtcgaagt cctcgccggc gacgagcgcg gggatgcgct
     1861 cggggtcctt ttcggtctgc actcgccggg cgtgcttctt cagaagcagc cggagcgcct
     1921 ggagcacgtc ttcgtcgtca tcgacgatga gcacgcgccc gaggagatcg gccatggggt
     1981 gttcggattc ggacaccccg tgtactgtgc cttggcccgg gaggttgcgc gccgggcccg
     2041 gcacgcccct cattcgtagc gaagcgaccg caccgggtcg aggttcgcgg cccggagagc
     2101 ctgccaggcg acggtcagcc atccgacgcc cacggccagg gacgcggccg cgagcgccgt
     2161 ccccaggccg ggcgtgatcc ggtaggcgaa gccgtcgagc cagcggtcca tgagcacgaa
     2221 cgagaccggg catccgacga ggacggccac gaccacgagc gccgtgaagt ccctcgacag
     2281 gagaaacacg atgtcgcgcg caccggcccc gagcaccttg cgcacgccga tttccttcgt
     2341 ccgctgctgc gcgacgaacg cggcgagccc gaagagcccg agacacgcga tgacgagggc
     2401 gaccgccgcg aacgcgtcga gcatgtcggc gagcctctgg tcctcttcga agtgccggtc
     2461 gaacgcgtcg tccaggaact gggcgtcgaa ggggaatccg ttcgagaacc gcttccagac
     2521 ctgctcgacc gcggcgagga cggccccggt gtcgcggccg tcgatccgga gcagcacgta
     2581 cgataggccg tccggccgga gcgagagcgc gagcggtccg atctcctgcc ggaacgagtg
     2641 gaaatggaaa tcccggacca cgccgacgac ttccatcggg gtgtccccga acccgacgtg
     2701 ccgccccacg gggtcctccc agccgaatgc gcgcgccgcg gtctcgttca ggatcaggcc
     2761 gcggtcggag tcggccgcga atccgggcga aaagctgcgc cccctgacca ggtcgagccc
     2821 cagcatctcc acgaacccgt agtcgacacg ggcgaggtac accctcatct cctgcccctc
     2881 ctcgcggccg tcccactccc gggtgccgtt gctctgctgg atgtcggtcg gaagatgcga
     2941 ggcgccggag acggccccga ctcccggcac ggcccgcatc tcgttcgtga gcgccgccca
     3001 gcgctcccgc acggaggaat cccggacccg gatggcgagc acctggtcgc gctccatccc
     3061 ggtatccgtc gacccggcga acccgatctg cccgtggacg accatcgtgc cgaagatgag
     3121 cccgatcccg atcgcaaact gggcgaccac gagcacgtcg cggagacgcg accggcgcga
     3181 tccgagcacc gggtcgcccc gaagaacggc cgcggctctc ggccgcgaga ggaccagcgc
     3241 ggggtagctc cccgacagga ctccgacgac gaggaccgcc gcgagggcca gcgcgatcgc
     3301 cggcgggtcc aggaggaagg acggcggaat gtcgcgcagc accagcgcgc tgaacgcggg
     3361 ccgcgccatc gccacgatcc ccgccgcggc gagcgccgat atcgcggcga ccaggaccga
     3421 ctctcccagg aactgcgcgg ccagctgccg ccggtccgcc ccgatcgcct tccgcacccc
     3481 gacctcgcgc cggcgcgtgg ccgcccgggc cgccgccagg ttcatgtagt tggtgcacgg
     3541 gatgagcagg accaggagcg cgagcgcgag cccccaggcg acctgcgtcg cgcttccgtt
     3601 cgccttcagc tcgaaattga tgcgcgactg caggtggatg tcccggatcg gctgcgcttg
     3661 cagcgtcgtc ttccggtccg gcccgccccg ataccaggat tcgccccgca ggtgggcgtc
     3721 cgcgagccca tcgatccgcc gggcgaaggc ctccgcgtcg gtgcccggcg cgagcacggc
     3781 gtaggtgtgc cacgagctcg agccccagtc atccaggctc tcctcgaagt ccggctccgt
     3841 gctcgccgag acgacgtacg cgtagccgat gtggctgttg tcgggcgggg gcccgacgat
     3901 gcccgtgacc tcgagggagt agctatcgcc gaagaactcg tactcgatcg tctctcccag
     3961 ggggtcttcg tccccgaaca ggcgggcggc cagggcgggt gtgaggagga cggcgccggg
     4021 tcgcgaaagg gcgccggccg cgtcgccgcg caggaggcgg aacgagaaga cgtcgaggaa
     4081 ggtgcccgtc gccaggagac cgcgcgagac gtcggtacgc tcgcccgccc ggagcagcgc
     4141 ctcgacccgc cggatccgcg tcgtcgcctc gaccccgggc acctcgtcga ggagggccgt
     4201 ctccaggggg gcgggcgtga cggcgaaccg gtcggtgccc atgaattcgt tgcccgcgtc
     4261 ccgcttgacc acgcggtacg tccggtcggc ccggtcgtgg aaggcgtcgt agctccgctc
     4321 gaagcggatc catgagacga tgaggaagaa gagcgcgagg ccgacggcga gccccaggac
     4381 gttcagggcg gaatagaatc cgtcccgccg gagggcgcgg aacgcggtct tcaggtagct
     4441 ccggagcatg ggcggcagcg gggggccggg aatggcctcc ggaacgcaaa tcgcgtacca
     4501 cgcccggcag gccgaccctt gcgccccctt ccgcccggcc cccttcggcc gcaggccgcg
     4561 aactgtccgg tatcggacaa cccgcgtccg cggccgggca ctatcgggcg tacgatcggc
     4621 gcgcggcggc gtttttcccc ggcggagcgt ctcggcacgg ggattgagtt gccctgcacg
     4681 acacgcacct acatccacca cgaatggccg agaccacccc agcgacaccc cccgggcgac
     4741 cgagcatcct gaccacgccg atcggctccg gctcggggaa ggcgacggga gagggcgtcg
     4801 atcgccgcct cgcgaagcgg ttctggacgg gccgccgcat cgcgatggtc gcgggcgggt
     4861 tcgtcgtgct ggcgctcgtg gtgtgggcct tcacgcagtc cctcgggggg cggcgcctcc
     4921 gcgtgcaggc cgagcgcctc acggtctcga ccgtcgagat cggcccgttc caggaatacg
     4981 tgcccgtgac gggcagcgtg atgccccgga cgaccgtcta cctggacgcg gtcgaaggcg
     5041 gccgcgtgga ggagatcttc gtcctcgagg gagcgacggt cgagcagggc acgccgctcc
     5101 tgaggctctc gaacaacgac cttcagctgc gcctgatcaa tgccgacgcg cagcggctcg
     5161 agcagatcaa ccgcgtgcag gacatgcaat tccgcatgga gcagaacgcg ctcgatctcc
     5221 ggcagcagct cgcccagatg gactaccaga tccagcgtct cgagcgcctg aacacgcgga
     5281 acgaggagct gctcgcgatc cagggcatct cgcggcagga gtacgactcg gtccgcgacg
     5341 agctcgacta ctggacaagg aaccgcacgc tgacgctcga aggctaccgc caggactcgc
     5401 tccggatgag cggccagctc atgcagatgc aggaatcggt ccgccgaatg gaggcgaact
     5461 acgacgtgat cgagagcatc ctgggcaacc tcacggtccg tgcgccggtc gccgggcagg
     5521 tgacggcgct caacgtcgag ctcggcgagc tgcatacgcc cggctcgcag ttcggccaga
     5581 tcgacatgct cgacggcttc aaggtccggg ccggggtgga cgagttctac atcgcgcgag
     5641 tgcaccgcga ccagccggcg acgaccctgc cgatcggaga cacggagtac ccgatggtcg
     5701 tgacccgcgt ctacccgcag gtaaccgacg gccggttcca ggtggacctc gagttcgagg
     5761 gcgagccccc gcccggcatc cggcgcggcc agtccgtccg gttccggctc gagatgagcg
     5821 agcccgccga ggccctgctc ctgccgatgg gcggcttctt ccaggcgacc ggcggcaact
     5881 ggatctacgt actcgacggc tcgggccagg cggtccggcg cgaaatccgc gtgggccggc
     5941 gcaacccgca gtatctggaa gtcgtcgagg gactcggacc cggcgaccgg gtggtcacct
     6001 ccggctacga cacgttcggc gatgccgagc ggctgattct agaatgacgg cccggccgtc
     6061 cccctttccc gaaagcaacc agacgatgat ccagaccgcc aatctcacca aggcctaccg
     6121 caccgacgaa gtggagacgg tggccctcgc cgacgtcacg atcgacatcg cgcccgggga
     6181 cttcgtctcc gtcatggggc cgtccggatg cggaaagtcc acgctcctga acatcctggg
     6241 catgctcgac aacccgacgt ccggctcgta ccggttcgac ggcacggagg tggccaacct
     6301 ctccgagcgg cagcgcgcgc gcctgcgcaa gggcaacatc ggcttcgtgt tccagtcgtt
     6361 caacctgatc gacgagctct cggtcttcga gaacgtggag ctgccgctcc tgtacctggg
     6421 cgtgtcggcg gacgagcgga aggcccgcgt gcaggcggcg ctcgagcgga ccgccatcgc
     6481 gcaccggcag gaccacttcc cccagcagct ctcgggcgga cagcagcagc gcgtggcgat
     6541 cgcccgggcg gtcgtggcga atcccggcct gatcctggcc gacgagccga ccggcaacct
     6601 ggactcgcgc aacggcgagg aggtgatgaa gctgctcgtg gaactgaacg aggccgggac
     6661 cacgatcgtg atggtgaccc actcgccggc ggacgccgag tacagccacc ggaccatcca
     6721 cctgttcgac ggccacgtcg tgaccgagaa cttcatggcg agccacgtgc cgggcgtgtg
     6781 agcggaggag actccgcacc aggctccgca aagaccgact ccgcagcatg atccggcact
     6841 acctcaccgt atcgttccgg cacctggccc ggcagaaggc ctatgccctc atcaacgtgt
     6901 tcggcctggc ggtcggcatc gcggcgtgct ggctgatctt cctgtacgtg caggacgagc
     6961 tgacccatga cgccttccac gagcgggccg accgcatctt ccgcgtgacc gaggtcgcct
     7021 acacgccgga cggtgccatc gagagccagt cggcctacct gccgtacccg atgaagccca
     7081 cgctggagaa cgagatcccg gaagtcgagg aggcgctccg caccgatccc gttacggtgc
     7141 ttctgcgcgt gggagaccgg acgttcgagc aggagatcct gtgcgcggac ccggagctct
     7201 tcaccgtctt ctcgttcccg ctcgccgcgg gcgaccccgc gtacgccctc gccgatcgca
     7261 acagcgtggt cctgaccgag aacatggccg cgaatctgtt ccccgacggg gatgccgtcg
     7321 ggcgcacggt cgaggtccgg ctgcgcgacc ggttcgagcc gctcacggtg accgccgtgg
     7381 cgcgcgacgt gccgggaggc acgagcatcc cgttccaggt cgtcgtgccc ttcgagaagc
     7441 tcgccgaggc ctcgggctgg atcggaaccc gcgacgaccg ctggaactcg tactcgttct
     7501 acacctgggt gctgcttcgc gagggggcta cggaggctgc gttcgaggcg aagatgccgg
     7561 cgctcctcga gaaataccgg cccgggattc gggagcgttt catcgagcag ggccggtggg
     7621 agggggaggg ccacccgcgc ggcttcctcc tgcagccact cggttccgtg cacctggatc
     7681 cgtcgatcgc gggcggcatc gtgccgccga gcgatccgcg ctatgcctgg atcctcggcg
     7741 gcatcgcgct cgccctgctg ctcatcgcgt gcatcaactt catgacgctc gcgataggcc
     7801 ggtccgccgg gcgggcccgg gaggtcggcg tgcgcaaggc gtccggcgcc cgtcggcggc
     7861 agctcatggc gcagttctgg ggtgagtccc tggtcatggc ggcgctcgcc ctcgtgctcg
     7921 gcctggcact ggcctccgtc gccctgccca cgttcaacga gctcgcgggc aagagcctcg
     7981 cgctcggatc cggatcgtgg ggcctgtcga tcggcgtcct ggccgggctg tgcctcgtgg
     8041 tgggcctggt ggccggctcg tacccggcgc tcgtgctctc gtcgttccgg cccgccgaca
     8101 cgctctaccg cacgccgcgc ctgggcggat cgaacgtcct gacccggtcg ctggtcgtag
     8161 tccagttcgc gctcgccgtg ggcctgatcg cgggaaccgg cgtcatgatg cggcaggtgc
     8221 ggttcctgca ggagcgcaac ctgggcttcg accgggagaa cctggtcgtg gtcgatcacc
     8281 agggcctgga gggtaggccc ctcgtggccc acctgcggcg cgagctggag tcccgggccg
     8341 atgtggtcgg cgtgacgggc gcgagcatgg cattcacgca tggctcggac cgccacggct
     8401 gggtgcacga ggggaaggag taccggtcct tcgtctacta cgtgcaggag gactacctgg
     8461 acgtcatggg catcccgctc ctcgaaggcc gcttcttcga cccggcccgg ccggccgact
     8521 ccacggccac ggtggtcgtg aacgaggcgt tcgtgcgcat gtggggctgg acggatcccg
     8581 tgggccggag ggtgcccggg tacgagggcg agccggtcgt catcggcgtc gtcgccgacg
     8641 tgcacaacca gtcgctgcac gaggagatcg cgcccatcat gctctcgaga agcccggaca
     8701 gctggtacgg caataccctg gtccgagtcg ccccgggcgg cgtggcgggt gcgctggcag
     8761 cgatcgaggc ggcatggctg cgcgcgtcgc cggagctgcc ggtgaagacc tccttcctgg
     8821 acgaggatct ggaccgccag tacgcgagcg agcgccggtg gagccgcata gtgggctggt
     8881 cgggcgccct ggccgtgctc gtggcctgcc tgggcctttt cggtctggcg gcgctgacgg
     8941 tcgcccggcg gaccaaggag atcggcatcc gcaaggtgct gggagcctcg ggcaccaggg
     9001 tcgcggcccg cctctcggcg gagttcgccc ggccggtcct ggtcgccagc gcccttgcga
     9061 ttcccgtggc gtggatcctg ctgcagcgct ggctcgaggg ctacgcctac gcggtgtcac
     9121 tgccgggggc cgtgatcgtg ggctcggccg cgatcgtgct cgctctcgcc gtggctaccg
     9181 tcggactgca gtcgatccgg gcggcccgcg ccaatcccgt ccacgccctc cgatacgaat
     9241 aggcccgacc catgaacgag cccctcctca gaatcgtcaa cgtcacgaag tcctaccacc
     9301 ggggcctcca ggcgtcccac gtgctgcggc gcgtgagctt cgacgtcgcc cgcggcgatt
     9361 tcgtcacgat catgggaccg tccggctcgg gcaagtcgac gctgctgcat atcctgggtc
     9421 tgctcgacga gcccagcgag ggggagtact ggttcgacgg ccaggccgcg cacgcgatcg
     9481 gcgagcgccg gcgcagcgag ttgcaccgcg ggcacatcgg cttcgtcttc caggcctacc
     9541 atctcatcga cgacctgacg gtctacgaga atctcgagac gccgctcatc taccgcggcc
     9601 tgtcgaaggc ggatcggcgc gggcgggtgg ccgagacgct ggaccggttc aacatggtcg
     9661 ccaagaagga cctgtttccg tccgagctgt cgggcgggca gcagcagctc gtcggcgtcg
     9721 cgcgcgcgct gatcatcgag ccgagcgtca ttctcgccga cgagcccacg ggcaatctcc
     9781 attcgagcca gggcgacgag atcatggagc agctccgccg cctgaaccgc gaccagggcg
     9841 tgaccatcgt ccaggtgacc cacaacgcgg cctacgccgg gtacggccac cgcatcatcg
     9901 agctgctcga cggccgcatc cgggacgatc ggcccgtcgg cgccgtgcat tccgctaccg
     9961 tcccgtaacc cagccccatc acctccatga gcgtcatctg catcccgatt ccggacctgc
    10021 agcgccaccg gaccgtaggt ctcgagatca gcgtcgacgg cgttcggcga gtcatgaact
    10081 accgggtgga gaccgtgccg tggcccccgg acctggaccc cgaggcccgc atcgagcagc
    10141 tccgctcgta cctccggatg cacgacccgg agtgggagct cgtgcagatc ggtccgcccg
    10201 acggcgaccg ggtgcccgtc acgttcaggc agcacgtggg catcgccacg gccccggcct
    10261 gatccgatcc gccaaaccgg caaccgaagg tcgtccggac ccatgctgcg aaactacctg
    10321 accgtcgcct tccgccgctt ccgccgcggc ggcacccacg tggccgttaa cgtgctcggc
    10381 ctcgcgctgg gcacggcgac ctgcgtgctg ctgttcctcg tggtctacac cgagtggagc
    10441 tacgaccggt tccacgagaa cgccgactcc atctaccgga cgtacttctg gtacgaggat
    10501 ccggacggga agccgggcat ccagaccatg atgccgccgg atttcacgcc cgagttcaag
    10561 gcgacctttc cggccgtggt cgaggccacg cggctcgtgc aatcggagcg tgactaccgc
    10621 caggacgaaa cgacccgccg gttcatgctc tcggaagtcg acccggactt cttccgcatg
    10681 ttctcgtttc cggccgtagc cggcgatccg gtcgcgacca tcgccgatcc acggggcatg
    10741 gtcatcacga ccgacgtggc ccgggccgcg ttcgggggag ttgcgccgga ctgggccgcg
    10801 atcctgggcc ggacgatcgt gatcccgctc gatacgaccg aga
//
LOCUS       DBNHQZ010000347        20773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2134814, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000347 DBNHQZ010000000
VERSION     DBNHQZ010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 20773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20773
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2134814"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16YU_09330"
     CDS             complement(<1..195)
                     /locus_tag="AC16YU_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6065468.1"
                     /translation="MTRRSATYEQLATAFRHGQFAPLYLLYGEERYFVDRLQALLLEK
                     AVAPHERDFNLDILYGRETDA"
     gene            complement(473..988)
                     /locus_tag="AC16YU_09335"
     CDS             complement(473..988)
                     /locus_tag="AC16YU_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065469.1"
                     /translation="MIRAEDREVLIGQVAYLVDELHAQEAILSFVPERLWSAGSPSGE
                     HSLLELYGLLVALGERSYPGLLEAWSEEGPRSADSVDEYDLRDSEPWNESTPRALVAR
                     AVRAREAVVARLEAAGEEEWAAVKTIDGRPATLGDIAFRITQDDAAVLRRIGELLHDA
                     NLGGSPVTRLS"
     gene            complement(985..1617)
                     /locus_tag="AC16YU_09340"
     CDS             complement(985..1617)
                     /locus_tag="AC16YU_09340"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR6065470.1"
                     /translation="MPGIPLNTARPNERKLNFWERIYLPEVLKGLGYSLGKFMKEPFY
                     TLQYPDEPWYPPDVFRGRPVLVEEDGRPRCVSCNLCARACPPLAISMKSRDIGGVKER
                     EPEWFEINMLRCIYCGFCEEVCPEEAIVMSKEYDLTFQSREEAVFGMERLLMPKERLK
                     DRLDWLEAQRNPQFGKRVDFRHDNNRHSLRDRPFLRHLAEEHAARPAAEA"
     gene            complement(1721..3022)
                     /gene="der"
                     /locus_tag="AC16YU_09345"
     CDS             complement(1721..3022)
                     /gene="der"
                     /locus_tag="AC16YU_09345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6065471.1"
                     /translation="MPLVAIVGRPNVGKSTFFNRVTETRDSIVDDMSGVTRDRVYGHA
                     EWQGRGFAVVDTGGFVPNTADRFEAAIREQVAIALDEADVIVLVVDVTTGVTDLDHEV
                     GQMLRKTEKPVFVLANKADNDERRWQAAEFWQLAVGEVFPVSSINGMGSGDLLDAVVK
                     ALPEAPPGEADERTRIAIIGRPNVGKSSLTNAWLGEERSIVTEISGTTRDSVDAILRY
                     HGRELVLVDTAGLRKRSRILENVEFYALLRTERAIESCDVAVLLIDANGGLEAQDIKV
                     LKMAEQQRKGLVIGINKWDLLEKETNTARDFERSIKERLRTLEYVPVVTISATTKQRV
                     SKLLETALAVEENRRRRIPTSELNAVVLPALEAHRPPSYRGQAVRIKYATQTRTEPPV
                     FVLFCNHPDGIKEPYRRYLENRLREAFDFTGVPLVLSFRQK"
     gene            complement(3084..3899)
                     /locus_tag="AC16YU_09350"
     CDS             complement(3084..3899)
                     /locus_tag="AC16YU_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HIR6065472.1"
                     /translation="MRLNEYERQARREIDAWQHGDASIVAQAMNWAMAPMDWVVRRIV
                     SEDLVEQVDEAVTQFLSMLNDASEWTLDASEVLADARRHGIDVARAEELRDHPLETLD
                     QIARGYTTQNAILAAVEGGGTGLGGAVLIAADIPLLFTINMRLIQQIGASYGFPLRGP
                     AYRPLVLSIFNVAASSSREAKNDALREISVAGSALARGQEYRGRVSGTFREQNRHVPR
                     EIAKNIVGRKLAQAIPIAGAAVGAGINYWFTTEAAEGSYMLFRALYLENKERG"
     gene            complement(3958..5436)
                     /locus_tag="AC16YU_09355"
     CDS             complement(3958..5436)
                     /locus_tag="AC16YU_09355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6065473.1"
                     /translation="MKYRIFVVDDDKHYARMLSYRLEKHPDYDVRVFASGEEVLRAME
                     DTPDLLLLDIMMPGIDGLEVLRRVKARRPTLPVVMVSAQGVIETAVESMKLGAYDYIT
                     KGQDDLVKLDTLVRNVLEKVSMERELATLRGEVSRAYSVDGMVGDSPAMKNVYRIIDK
                     ALRGDLTVAIIGESGTGKELVAKAIHFNSARKRGPFVVVNCAAIPRELMESEFFGHEK
                     GSFTGAHARKIGKFEQADGGTIFLDEIGELNLDLQSKLLRALQEGEISRVGGNDTIRF
                     DARVISATNRDIVAMIRAGKFREDLYYRLFQYPVPLPPLRERGQDILHLANHFLREYT
                     STHPDFRGRKLSVAAKQAISGYAWPGNVRELKSAIERAVLLADGDEISSMDLMLGDQG
                     RLVSWKDRMSLAAATAHAFDEEPQLPVNGNGTLHHDDQESLDGADSGNGADSIVSLDE
                     LKRRAVERAYRLCEQNVDRAAVELGIGRATMYRLLKRYELMP"
     gene            complement(5561..6370)
                     /locus_tag="AC16YU_09360"
     CDS             complement(5561..6370)
                     /locus_tag="AC16YU_09360"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6065474.1"
                     /translation="MGWWEAALLGLVQGLTEFLPVSSSGHLVIGRHVLGLAATDDVVF
                     EVFVHFGTVMSIVAVYREEILRLVKATLGALVAPRAAASRWSGDEDLRIVVFILLTLV
                     PTGIGYVLFRDPIEAAFADPRLASGMLIVTGVLLLLTMLRRAPSGELTAWRSLVIGVA
                     QTFAMIPGISRSGSTICAAIYLDVKPEKAANFSFLMLLPVVIGATLLKTAEVIEQGVA
                     DWVPLVLGTVVAFASGVVAIRVVLGFVRRGRLQVFAWYCFAVGALGLLLIR"
     gene            complement(6419..8077)
                     /locus_tag="AC16YU_09365"
     CDS             complement(6419..8077)
                     /locus_tag="AC16YU_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HIR6065475.1"
                     /translation="MVDGWYVLRALASEWDRSMAGATLEDVYSAARDELSLVFAGGDE
                     EHTLRISVRPGDRFVFRQPGGARPRRNVSTLFGGALRRALVAVRIADRDRVLDLDLAD
                     GHVIRVLLYGPRANVMLGRGGVVEASFREKLAAPGTPMPEPRPAPEVLGARALAGRWP
                     SGSQEMARAVARAYPLFDPLVAAEVVRRAEPAVSDACLAGADDLERIARAAEGVRSEL
                     LAPRPRVYRDGRAPVALSLLPLDPPPAPEEETFESVDAAVRSFVRARLAHGAFRAEFE
                     PIRRTLEAAAVRNRTRADRMLRELAGTSRADRYERWGHLLMAARPETRGHDSVDLPDL
                     FAEGETARIPVEPAIGAVENAERLYDKARQTRRAREEAELRLGAVDSARREAERLLAE
                     LQGIEDVRALRAFRTGHADALARFGRERPGDAPAMPFRRFDLGGGYEAWVGRSAKEND
                     ELTFTHARRHDWWLHARGAAGSHVVLRCPRRAERPPHRVVEAAAAIAAWFSKASGSAM
                     VPVIVAERRFVRKPRGAAPGAVVVDREEVVIVEPRLPAQAPDDL"
     gene            complement(8070..8393)
                     /locus_tag="AC16YU_09370"
     CDS             complement(8070..8393)
                     /locus_tag="AC16YU_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065476.1"
                     /translation="MGWLRHVIIDLAITLCVVLATFFAQGWAAWVVGIYTPAMVLLKI
                     GAIGAKLPQPKDGPPDWFFHAIYAVNVVILLYHGWYWVAAGWVVIWVLSALAAARNRI
                     PTKRG"
     gene            8461..9663
                     /locus_tag="AC16YU_09375"
     CDS             8461..9663
                     /locus_tag="AC16YU_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6065477.1"
                     /translation="MHRLPALLLAGVLAACGLSSDGPRPATDSASVQAAGAPPAAGPD
                     TAAAVRAAAVDSVVGAAASQDVPAADTQSTAALPALVTMSRSELQALLRAAAQGTRDT
                     IVVRMDTASAAAPDTSVAEGVSSIQEGLRFLGARFFFAIVVFFATYWVLRILVWLIES
                     LAERSARRRLLLKRMIPITRITAWSLAVYFVVANVFEVDRNGILAASAALGIGVGLAA
                     QDVLKNILGGLIIILDQPFQVGDKVAVGGTYGEVVSIGLRSTRIVTHEDSLVSVPNAQ
                     VVDSQVANSNSGALDCQVVTHLYLPGWTDVEKAKRIAYNAAANSRYVFLDKPIVVLVK
                     DEFKETFLTHFLVKAYVLDTRYETALSSDITETAKIEFLKAGLLEPVYGLTAPPELMG
                     TGARMDPQ"
     gene            9660..12845
                     /locus_tag="AC16YU_09380"
     CDS             9660..12845
                     /locus_tag="AC16YU_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065478.1"
                     /translation="MNATARPLTDPADAEHVEALARLRAEAAAFVAGAGDAVDAWRSA
                     VEEDVVGALARRCRDLAEFHAGLADGLPGPDDERRLAWDAIRAYAGATAREVWAVVAD
                     ELDAMAVLGGLSDRSDDTLRTLEEMAAGLSGPTVVPLDPALFEPSEGDGALTRLHKGL
                     FRFRRRLRLGRARMLGALGSVFGRDRVTGPAPVRALSLESHALAHLRGTVRNDLLSAE
                     SAARDALSEALSRVQVLASQWARAVLSALGRTDRGAWHVPGEGVPVVAVSPEVDDEAW
                     SEALSVLGLAAESVTAELRSIADEVDAVAVELSVRGGLETVLLRALREGGTVLHEGPE
                     PRPDSSATSRPPVRGSGDRSRLAAEIVTFRVELADAETGFHRAVAEGVLAPLAQSGQE
                     IRDMLSRIGAETLERVDEGEGSDALGQIHAAAVDRLAQMVRNLPQPERAEEVFRTAAE
                     RAQSAVTNRIGTLPDELRLPVGPGRHDRLAPMALRRAAALAFERDLVPRIMAAYRPVQ
                     RELVRTWNDSEHLLQVVDSTFGAAQEELDAAQGDDAAERRESAHGLIEDGLRRAAEIL
                     EDGYESLGAPWTGLAESVHRVLRDDWMHLEGAAQADDFLAKHWQGLRARLKRRAEHAL
                     RTAGEDWERLSGNAIVLFRAARIRIARLVRLGRAAVGVAEPQATERIRTLEAVTGAAE
                     LYAGLPLIYRKLFTLDPLSEKGLAVNRQGDLSAVVDAFERWNGGTGPGLFIANSEPGS
                     GLTSFLNLAARTLADRARIVRVPIPERVDDESVLARHLGRALGLGTEGPETLSDLEEA
                     IQTSDFQTGVVLVDDLEHVMLQTPGGTRLIEDLLLFFARTDASLFWCASIGDLAWRFL
                     ERTVPAATGLATGHALDSWSRKAIQALILERHRRTGMPLRFEAPDDPAPILRQRLRRA
                     RTPEQTQRLLRDEFFDALFRSAGQDPALALYGWVRMGRFDEEGDELTLPPFRPLSFSF
                     LGHMDNDRAFVLRAFLIHHTLSPEELARIFRFRPDQAMANLETLLALGLIEPVTEGAP
                     VSYVVSGMRYRLRRLVLQPVRRYLAERHFVY"
     gene            complement(12879..13718)
                     /locus_tag="AC16YU_09385"
     CDS             complement(12879..13718)
                     /locus_tag="AC16YU_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6065479.1"
                     /translation="MYVPRQQRMLDQYLQEIGQIPLLTPQEEVDLARRIKAGDQDALH
                     RLTRANLRFVVSVAKKYQGQGLTLADLINEGNYGLIKAAQRFDETRGFKFISYAVWWI
                     RQAILQALAEQSRVVRLPLNRIGTISKIRKTSARLAQVHERQPNIEELAQELEVDVEK
                     VREAMQHTGRHLSMDAPFNEDDDNSLLDVLPNDDDLSPDDTLMDESLKIDIERALSLL
                     HPREAEITRLYFGIGREHPLTLEEIGQRFGLTRERVRQIKEKALRKLRQKHRREELQM
                     HIG"
     gene            complement(13862..15439)
                     /locus_tag="AC16YU_09390"
     CDS             complement(13862..15439)
                     /locus_tag="AC16YU_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 family acyl-acid amido synthetase"
                     /protein_id="HIR6065480.1"
                     /translation="MRLAQGYLSMSVASALKQLPMIRALDRALADPAASQERLLRGLL
                     HEARDTEWGRRFGFAEIARTAGVTAAFRDRVPLHDYEAYRDDVRRVREGAKDVFWPGS
                     IRHFAVSSGTVSAGKIIPLSPDMLRRTRDYTLGMALRYAMATGKYGWMGGRLLTVPGR
                     VEPDVGGPGTWVGEVSGLMYLAAPWPIRRFRQAVHRDVLFTDGWDRKLRMIADRTCRM
                     DIRAMAMVPSWAVVLFPLVVERYNELTGGRATCVRDVWPNLEVFFSGAVALSSYEDLM
                     RRQIGGSAPMHFVEGYGASEGPFAFQGHPDERDMLLHLASGVYYEFVRMDDTSDAPLR
                     HTLAEVEPDVRYRICVSTCSGLWTYGVGDVIRFTGTRPHRIVVAGRTNEMLDRYGEAV
                     FGEEARAALESACAGAGAHLRDYHVTSVPPVEGRMPRHRWLVEFAGPAPDTERIATAI
                     DTYLQRVNRHYVIRRECGAFERPEIVALPEGTFLRWLRATRDHVDAQTKVPRMSEDPR
                     TADGVLEIAETITEPSS"
     gene            15562..16500
                     /gene="folP"
                     /locus_tag="AC16YU_09395"
     CDS             15562..16500
                     /gene="folP"
                     /locus_tag="AC16YU_09395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6065481.1"
                     /translation="MPAPHRDPTPDPFVLDCRGRTVDARPGRAVIMGILNVTPDSFWD
                     GGRYVEPDAALRRAVQMLDEGATIVDVGGASSRPAGRTYGEGARAVPPDEETLRVVPV
                     IEAIARERPDAVISVDTVHAAVASAALDAGAHLVNDVTGLRNDPELARVAAGAGAGLV
                     LMHSVGAWGALEQDVMQGDVVARVAGSLERSRDLALEAGVRSLVLDPGFGFGKSPAQN
                     LRLLARTGAFLELGHPVLAAVSRKSTIGIALGSADLPRPVAERLYGSLGATAVAVMRG
                     ASIVRTHDVAATRDFLAVLLGTLAAGDDGAAWSGPS"
     gene            16532..17341
                     /gene="cdaA"
                     /locus_tag="AC16YU_09400"
     CDS             16532..17341
                     /gene="cdaA"
                     /locus_tag="AC16YU_09400"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR6065482.1"
                     /translation="MTIFPWIIPIRVVDLVEIALVAYVLYKLYLWMRGTIAVQIFAGI
                     LALYAIQVIVTAADMTMLRAIFGSVSEVFVLAVIILFQPEIRRLLLILGQNRLVRGFM
                     TVPTRNEAIAEVAGAVTEMSNRRLGALIAIERSTGLRSYVETGERLQAEVSRDLLVTI
                     FYARNPLHDGAVIIRSGRIEAARCILPVSTSPRLSPNLGLRHRAAVGLTEQTDAFVIV
                     VSEETGAISIADGGTLTQGVTSTQLRVRLQEALSSGPPAPSVEPAPEDVDE"
     gene            17338..17991
                     /locus_tag="AC16YU_09405"
     CDS             17338..17991
                     /locus_tag="AC16YU_09405"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6065483.1"
                     /translation="MTVTRPYQRRREELVEILRDKGITDERVLEAVRRVPRHEFVDHA
                     LVRRAYEDEALPIGLGQTISQPFTVAYQTVLLGVRPGDRILEVGTGSGYQAAILCELG
                     ALVFSIERLRPLYVRTRHLLRDLGYQVTCRLGDGSQGWEAKGPFDGIVVTAGAHTVPD
                     PLVQQLREPDEDSPGGRLVIPVGGHAGQTMNRIVRTGAESWERERTDGFRFVPLIPD"
     gene            complement(17996..18298)
                     /locus_tag="AC16YU_09410"
     CDS             complement(17996..18298)
                     /locus_tag="AC16YU_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR6065484.1"
                     /translation="MNLGRVIGSVWATRKVASFEGRRLMVVQPLTFDREPAGDPFVAI
                     DTVDSGVGDYVMVVTSTEATVPFKPLPTPTDAAIVGVVEQVETERGTWSAPGRDEP"
     gene            complement(18295..18570)
                     /locus_tag="AC16YU_09415"
     CDS             complement(18295..18570)
                     /locus_tag="AC16YU_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR6065485.1"
                     /translation="MVLGKVVGTVWATRKDEQLTGLTLQIVREIALDGSSRDRFVVAV
                     DSVGAGIGETVLMAQGSSARQTDLTRNRPVDAVITAIVDRVDAEGAV"
     gene            complement(18677..19858)
                     /locus_tag="AC16YU_09420"
     CDS             complement(18677..19858)
                     /locus_tag="AC16YU_09420"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR6065486.1"
                     /translation="MRHPGVTTGHLFVTIVLDGVGIGAQPDAARYGDAESDTLGHVCA
                     ARRPALPNLAAFGLGCIRPLDGVPCAGAPGASWGRMLETAAGKDSTTGHWELAGLALM
                     EPFPTYPDGFPDDVVRAFCREAGVDAVLANRPASGTTVLDEEGARHLETGRPIVYTSA
                     DSVFQVAAHVDVVPLERLYDWCASARTRVCVGRHAVGRVIARPFEGEPGAFVRLSESR
                     RDLALPPPGPTLPSVLSAAGIRTVSVGKVADLFAGVGFDETVKTKSNADGVEGLHGAI
                     RASRGRPTFVWANLVDFDQDFGHRNDAEGFARALEAFDRALPGLVEALPEGARLVLTA
                     DHGNDPTTPGTDHAREYVPLLSWDGRPGRPLGTRSTFADHAAAAAAYFGVPFECEGRA
                     F"
     gene            complement(19840..>20773)
                     /locus_tag="AC16YU_09425"
     CDS             complement(19840..>20773)
                     /locus_tag="AC16YU_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065487.1"
                     /translation="ARILVEGVSDAFDGEVMLSVLRGLRAAVDATTVCLLRQEEGALS
                     YSVEAVVSRNAFARNGGRFAVTEPLLAGHKPLEPVVLSCVAPGGFEPKRLGYYHETIA
                     IRQVAFVPMSGQDGRAAWLLVADSMGDSGLERTAATRALAEFGRLVHSLVSRGMTVPS
                     RSTPAGVPLRPRREIIADEMEAARTGRAPLALALVYLNRAEKIGESGDETLREVDEVF
                     HERLKGATSDGRVERFGELTYGVFRRVEVDDAARWASDLQARMAGEGGLLEGGVSVGV
                     AMLGDRHDGPDQLRSDATAALRESYESGECVILE"
BASE COUNT         2988 a         7406 c         7280 g         3099 t
ORIGIN      
        1 ggcgtccgtc tcgcgtccgt acaggatgtc caggttgaag tcgcgctcgt gcggcgcgac
       61 cgccttctcg agcaggaggg cctgcaggcg gtccacgaag taccgctcct cgccgtacag
      121 gaggtacagc ggggcgaact gcccgtgtct gaaggccgtc gcgagctgtt cgtatgtggc
      181 gcttcgccgc gtcatctcgc agtagggggt gcggagacag gatagggtcg ggggatgaca
      241 atctcccgtc acgttcccat gaccaaggaa cgcacgaaat gccctgtatc gtatgtgacg
      301 ggttccgggc ggtcgccacg ccaccctgag cggcgctgta cgcgggtccg gcgggtcgcc
      361 acgccaccct gagcggcgct gtacgcgggt ccggccggtc gccacgcgcc ctgaacggcg
      421 cggaacgctg ccccggcggg ccgccacgcg cgccgagcgg caccccggcg gttcaggaca
      481 gccgcgtcac cggcgagccg ccgaggttgg catcgtgaag gagctctccg atgcggcgca
      541 ggacggccgc gtcgtcctgg gtgatgcgga aggcgatgtc gccgagggtc gcgggccggc
      601 cgtcgatcgt cttcacggcc gcccactctt cctcgccggc cgcctccagg cgggccacga
      661 cggcctcccg ggcccggacg gcgcgcgcga cgagcgcacg gggcgtcgac tcgttccagg
      721 gctccgagtc gcggagatcg tattcgtcga ccgagtcggc cgaccgtgga ccctcctcgc
      781 tccacgcctc gaggaggccg ggatacgacc gctcgccgag cgcgacgaga aggccgtaga
      841 gctcgagcag gctgtgctcg cccgacggcg agccggccga ccacaggcgc tcgggcacga
      901 acgacaggat cgcctcctgg gcgtgcaact cgtcgaccag gtatgcgacc tggccgatca
      961 gcacctcgcg gtcctccgcc cggatcacgc ctcggccgcc ggccgcgccg cgtgctcctc
     1021 ggccaggtgc cgcaggaagg gccggtcacg caggctgtgc cggttgttgt cgtgccggaa
     1081 gtccacccgc ttgccgaact gcggattgcg ctgcgcctcg agccagtcca gccgatcctt
     1141 gagccgctcc ttgggcatga gcagccgctc catgccgaac acggcctctt cccggctctg
     1201 gaacgtgagg tcgtactcct tggacatgac gatggcctct tccgggcaca cctcctcgca
     1261 gaagccgcag tagatgcagc ggagcatgtt gatctcgaac cactcgggct cgcgttcctt
     1321 gacgccgccg atgtcgcgcg acttcatcga gatggccaac ggcgggcacg cccgcgcgca
     1381 caggttgcac gacacgcagc ggggccgtcc gtcctcctcc accagcacgg gccgcccgcg
     1441 gaacacgtcc ggcggatacc acggctcgtc ggggtactgc agcgtgtaga acggctcctt
     1501 catgaacttg cccagcgagt agccgagccc cttcaggact tcggggaggt agatgcgctc
     1561 ccagaagttg agcttgcgtt cgttgggccg agcggtattg agcgggatgc cgggcatgac
     1621 gcggggatgc gtgtgggacg cgggaatgta cggtgcccgc cggactgcga tccggccggg
     1681 cgggcattcg acggggtttt caaaatgggg gaagaccccc ctacttctgc ctgaacgaga
     1741 gcacgagcgg cacgcccgtg aagtcgaagg cctcgcgcag gcggttctcg aggtagcgcc
     1801 ggtacggctc cttgatgccg tccgggtggt tgcagaatag gacgaagacg gggggctccg
     1861 tgcgggtctg cgtcgcgtac ttgatgcgca cggcctggcc gcggtaggac ggcgggcggt
     1921 gcgcctcgag cgcggggagc acgacggcgt tgagctcgct cgtcgggatg cgccgccgcc
     1981 tgttctcctc gacggccagc gcggtttcca ggagcttcga gacgcgctgc ttcgtcgtag
     2041 ccgagatcgt cacgaccggc acgtactcga gcgtgcgcag gcgctccttg atcgaccgct
     2101 cgaagtcgcg ggcggtgttc gtctccttct cgaggaggtc ccacttgttg atgccgatca
     2161 ccaggccctt gcgctgctgc tcggccatct tcaggacctt gatgtcctgc gcctccaggc
     2221 cgccgttcgc gtcgatgagc agcacggcga cgtcgcacga ttcgatggcc cgctccgtcc
     2281 gcaggagcgc gtagaactcc acgttctcca ggatgcggct ccgcttgcgc agcccggccg
     2341 tgtccacgag gaccagctcg cggccgtggt accggaggat cgcgtccacg gagtcgcgcg
     2401 tggtgcccga gatctcggtc acgatggagc gctcctcgcc cagccaggcg ttggtgagcg
     2461 acgacttgcc cacgttcggg cggccgatga tggcgatgcg cgtccgctcg tcggcctcgc
     2521 cgggcggcgc ctcgggcagg gccttgacga cggcgtcgag caggtcgccc gagcccatcc
     2581 cgttgatcga gctcacggga aagacctcgc ccacggccag ctgccagaac tcggcggcct
     2641 gccagcggcg ctcgtcgttg tcggccttgt tcgcgagcac gaagacgggc ttctcggtct
     2701 tgcggagcat ctggccgacc tcgtggtcca ggtcggtcac gccggtcgtg acgtccacga
     2761 ccaggacgat cacgtcggcc tcgtccagcg cgatggccac ctgctcgcgg atcgcggcct
     2821 cgaagcggtc ggccgtgttc ggcacgaacc cgccggtgtc caccacggcg aagccccggc
     2881 cctgccactc ggcgtggccg tacacgcggt cgcgcgtgac gccggacatg tcgtcgacga
     2941 tcgagtcacg ggtttccgtg acgcgattga agaaggtcga cttgccgacg ttcggtcgtc
     3001 cgacgatggc gacgaggggc atggctcgct gtggagaagg gcgctcgttc acgcgcccgg
     3061 ctccggatgg atcccccggc ggctcagccg cgctccttgt tctcgaggta cagggcccgg
     3121 aacagcatgt acgagccctc ggccgcctcg gtggtgaacc agtagttgat gccggccccc
     3181 acggccgctc cggcgatcgg gatggcctgc gccagcttcc gccccacgat gttcttcgcg
     3241 atctcgcgcg gcacgtgccg gttctgctcg cggaacgtgc cgctcacccg cccccggtac
     3301 tcctggcccc gggcgagcgc cgagccggcg accgagattt cgcgcagggc gtcgttcttg
     3361 gcctcgcggc tggacgaggc cgcgacgttg aagatcgaca gcaccagcgg ccggtacgcc
     3421 gggccccgca gcgggaagcc gtacgaggcc ccgatctgct ggatgagccg catgttgatc
     3481 gtgaagagca gcgggatgtc ggcggcgatc agcacggcgc cccccaggcc cgttcccccg
     3541 ccctctacgg cggccaggat ggcgttctgc gtcgtgtagc cgcgggcgat ctggtccagg
     3601 gtctcgagcg ggtggtcgcg cagctcctcg gcgcgcgcga cgtcgatgcc gtggcggcgg
     3661 gcgtcggcca ggacctcgga ggcgtcgagc gtccactccg aggcgtcgtt cagcatggac
     3721 aggaactggg tgacggcctc gtccacctgc tccaccaggt cctccgagac gatccgacgg
     3781 accacccagt ccatcggcgc catggcccag ttcatcgcct gggccacgat ggaggcatcg
     3841 ccgtgctgcc aggcgtcgat ctcgcggcgg gcctgccgct cgtattcgtt gagacgcatg
     3901 tcggaattcc ggattcgccg atgcaagata gggcgacccg gaacagcggc atcgccccta
     3961 cggcatgagc tcgtagcgct tgagcaggcg atacatggtc gcccggccga tgccgagctc
     4021 gaccgcggca cggtccacgt tctgctcgca gagccggtac gcgcgctcga ccgcgcggcg
     4081 cttcagctcg tcgagcgaca cgatggagtc cgcgccgttg cccgaatcgg caccgtccag
     4141 ggattcctgg tcgtcgtggt gcagtgtacc gttcccattc acggggagtt gcggctcctc
     4201 gtcgaaggcg tgcgcggtcg cggcggccag cgacatgcgg tccttccacg agaccaggcg
     4261 gccctggtcg ccgagcatca ggtccatgga cgagatctcg tcgccgtcgg cgagcaggac
     4321 ggcccgctcg atcgcgctct tgagctcgcg cacgttgccc ggccacgcgt agcccgagat
     4381 ggcctgcttc gcggcgaccg acagcttgcg cccgcggaaa tcgggatgcg tgctcgtgta
     4441 ttcgcgcagg aagtggtttg ccaggtgcag gatgtcctgg ccccgctcgc gcagcggcgg
     4501 cagaggcacg ggatactgga acagccggta atacaggtcc tcgcggaact tgccggcgcg
     4561 gatcatcgcg acgatgtcgc gattcgtggc cgagatcacc cgggcgtcga agcggatcgt
     4621 gtcgttgccg ccgacccggc tgatctcgcc ctcctgcagc gcgcgcagca gcttggactg
     4681 caggtccagg ttcagctcgc cgatctcgtc caggaagatg gtgccgccgt cggcctgctc
     4741 gaacttgccg atcttgcggg cgtgcgcccc ggtgaaggag cccttctcgt gcccgaagaa
     4801 ttcgctctcc atgagctcgc gcgggatcgc ggcgcagttc accaccacga agggcccgcg
     4861 cttgcgggcg ctgttgaagt ggatggcctt cgcgacgagc tccttccccg tgccggattc
     4921 cccgatgatg gccaccgtca ggtcgccccg cagcgccttg tcgatgatcc ggtacacgtt
     4981 cttcatggcc gggctgtcgc cgaccatgcc gtcgacgctg taggcgcggg agacctcgcc
     5041 gcggagcgtg gcgagctcgc gctccatcga caccttctcg agcacgttgc gcacgagggt
     5101 gtcgagcttc accaagtcgt cctgaccctt ggtgatgtag tcgtaggcgc ccagtttcat
     5161 ggactcgacc gccgtctcga tcacgccctg ggcggagacc atcaccacgg gcagcgtcgg
     5221 tcggcgcgcc ttgacccggc gcagcacctc caggccgtcg atgcccggca tcatgatgtc
     5281 gagcagcaga aggtcgggcg tgtcctccat cgcgcggagc acttcctcgc cgctcgcaaa
     5341 cacgcggacg tcgtagtccg gatgcttttc caggcgatag ctgagcatcc gggcgtagtg
     5401 cttgtcgtca tcgacgacga aaatgcggta cttcatgcga tcggcggctc gttcggagcc
     5461 cgtcggtcga cggacgtcca cgcggaggta tcgaccatcc gtccgaatct taaaatgaga
     5521 caaccgaccg gaatcgcgtc cgtggcagag tccttgcggc tcaacggatg agaaggagcc
     5581 ccagggcgcc gaccgcgaag cagtaccacg cgaagacctg caggcggccg cgccgcacga
     5641 agccgagcac cacccggatc gcgacgaccc ccgacgcgaa ggccaccacg gtgcccagca
     5701 cgaggggcac ccagtccgcg acgccctgct cgatcacctc ggcggtcttg agaagcgtcg
     5761 ccccgatgac gacgggcagg agcatcagga acgagaagtt ggccgccttc tcgggcttca
     5821 cgtccaggta gatggccgcg cagatcgtgg agcccgagcg cgagatgccg gggatcatgg
     5881 cgaaggtctg cgcgacgccg atgaccaggc tccgccaggc cgtgagctcg cccgagggcg
     5941 cccggcgcag catcgtgagg agcagcagca cgcccgtcac gatgagcatg ccgctcgcga
     6001 gccgcggatc cgcgaaggcc gcctcgatgg gatcccggaa taggacgtag ccgatgcccg
     6061 tcggaacgag cgtcagcagg atgaacacga cgatcctcag gtcctcgtcc ccgctccatc
     6121 gcgaggccgc cgcgcgcggg gccacgagcg ccccgagcgt cgccttcacg agccgcagga
     6181 tctcttcccg gtacaccgcg acgatgctca tcaccgtccc gaagtgcacg aagacctcga
     6241 agaccacgtc gtccgtcgcg gcgagcccca ggacgtggcg cccgatcacc aggtgccccg
     6301 acgaggacac cggcaggaat tccgtcagtc cctggaccag gcccagcagg gccgcctccc
     6361 accatcccat gagcggtacc cggttcgatg cggccgaagg tacggccttc gcctccccct
     6421 acaaatcgtc cggcgcctgc gcgggaagcc gcggctccac gatgacgacc tcctcccggt
     6481 ccaccacgac ggcgccgggg gccgcgcccc tcggcttgcg gacgaagcgc cgttcggcca
     6541 cgattaccgg caccatggcc gagccggacg ccttgctgaa ccaggccgcg atcgccgccg
     6601 ccgcctccac gacccggtgc ggcgggcgct ccgctcggcg cgggcagcgc agcaccacgt
     6661 gcgatccggc cgcgccccgg gcatgcagcc accagtcgtg gcggcgggca tgggtgaagg
     6721 tcagctcgtc gttctccttc gcgctgcgtc ccacccaggc ctcgtatccc ccgcccaggt
     6781 cgaatcgccg gaacggcatt gcgggcgcgt cgccgggccg ctcgcggccg aagcgggcga
     6841 gcgcgtccgc gtgcccggtg cggaaggcgc gcagtgcccg cacgtcctcg atgccctgga
     6901 gctcggcgag caggcgctcc gcctcgcgcc gcgccgagtc cacggcaccg agcctcagtt
     6961 cggcctcctc gcgggcgcgc cgggtctggc gcgccttgtc gtacaaccgc tcggcgttct
     7021 cgacggcacc gatcgccggc tccaccggga tccgggcagt ctcgccttcg gcaaagaggt
     7081 ccgggaggtc caccgaatcg tggccgcggg tttccggccg cgcggccatg agcaggtggc
     7141 cccagcgctc gtagcggtcc gcgcgcgagg tcccggcgag ctcgcgcagc atgcggtcgg
     7201 cgcgcgtccg gttgcggacg gccgccgcct cgagcgtgcg gcggatgggt tcgaactcgg
     7261 cccggaacgc cccgtgcgcg aggcgcgcgc gcacgaacga gcgcacggcc gcgtccaccg
     7321 attcgaacgt ctcctcctct ggcgcgggcg gcgggtcgag cggaaggagc gagagcgcca
     7381 ccggcgcccg gccgtcgcgg tacacgcgcg gacgcggcgc gaggagctcg gagcgcaccc
     7441 cctcggccgc acgggcgatg cgctcgaggt cgtccgcccc cgcgaggcat gcgtcggaca
     7501 cggccggctc cgcccgcctg accacctcgg cggcgacgag gggatcgaag agcgggtagg
     7561 cccgcgcgac cgcccgggcc atctcctgcg atccgctcgg ccagcgcccg gcgagcgccc
     7621 gcgcgccgag cacctcgggc gccggccggg gctcgggcat cggggtgccc ggggcggcca
     7681 gcttctcgcg gaacgaggcc tccacgaccc ctccccggcc cagcatgacg ttggcgcgcg
     7741 ggccgtagag cagcacgcgg atcacgtgcc cgtcggccag gtccaggtcc agcacccggt
     7801 cgcggtccgc gatgcggacg gccacgagcg cccggcggag cgcgccgccg aacagcgtcg
     7861 agacgttgcg ccgtggcctc gcccctcccg gctgccggaa cacgaagcgg tcccccgggc
     7921 gcaccgagat gcggagggta tgctcctcgt ctccgcccgc gaaaaccagc gagagctcgt
     7981 ccctcgcggc ggaatacacg tcctcgagcg tcgccccggc catcgagcgg tcccactccg
     8041 aggccagggc acggagcacg taccagccgt caaccacgct tcgtcgggat gcggttgcgg
     8101 gccgctgcca gcgcggacag gacccagatc acgacccagc ccgccgccac ccagtaccag
     8161 ccgtggtaga gcaggatgac cacgttcacc gcgtagatcg cgtggaagaa ccagtccggc
     8221 ggcccgtcct tcggctgcgg cagcttcgcg ccgatcgccc cgatcttcag gaggaccatg
     8281 gccggcgtgt agatccccac cacccacgcg gcccagccct gggcgaagaa cgtggcgagc
     8341 acaacgcaca gcgtgatcgc gaggtcgatg atgacgtgtc tcaaccagcc catggcacgg
     8401 atcccgtgtt tataatgatc tccacgcaag gtatatacga cccggcatat ccccgaatcc
     8461 gtgcatcgac tgcccgccct gctacttgcc ggcgtcctgg ccgcgtgcgg cctttcgtcc
     8521 gacggaccac gacccgcgac cgactcggcg tcggtccagg cggccggcgc gccgcccgcc
     8581 gcgggtcccg acacggcggc cgcagtgcgg gccgccgcgg tcgactcggt ggtcggggcg
     8641 gcggcttcgc aggacgtccc ggcggccgac acgcagtcca cggccgcgct gcccgccctg
     8701 gtgacgatga gccggagcga actgcaggcc ctgctccggg ccgccgccca gggcacgcgc
     8761 gacacgatcg tcgtccggat ggacaccgcc tccgccgcgg cgcccgatac gtcggtcgcg
     8821 gagggagtct ccagcatcca ggaagggctt cgcttcctgg gcgcccggtt cttcttcgcc
     8881 atcgtggtct tcttcgccac gtactgggtg ctccgcatcc tggtctggct catcgagtcc
     8941 ctggccgaac ggtcggcccg gcgccgcctg ctgctgaagc ggatgatccc gatcacgagg
     9001 atcaccgcct ggagcctcgc cgtctatttc gtggtggcca acgtgttcga ggtggaccga
     9061 aacggcatcc tcgccgcttc ggccgcgctg ggcatcggcg tgggtctcgc ggcccaggac
     9121 gtgctcaaga acatcctggg cgggctcatc atcatcctgg accagccgtt ccaggtgggt
     9181 gacaaggtgg cggtcggcgg gacgtacggc gaggtggtct cgatcgggct ccggtccacc
     9241 cgaatcgtca cccacgagga cagcctggtc tccgtcccga acgcccaggt ggtcgacagc
     9301 caggtcgcga actcgaattc gggggccctg gactgccagg tggtcacgca tctgtacctg
     9361 ccgggctgga cagatgtcga gaaggcgaag cggatcgcgt acaacgcggc ggcgaattcg
     9421 cgctacgtgt tcctggacaa gcccatcgtg gtcctggtga aggacgagtt caaggagacc
     9481 ttcctgaccc acttcctggt gaaggcctac gtgctggaca cccgctacga gaccgcgctc
     9541 tcgagcgaca tcaccgagac cgcgaagatc gaattcctga aggccgggct gctcgagccc
     9601 gtctacggac tgaccgcgcc gccggagctc atggggaccg gggcgaggat ggatccgcaa
     9661 tgaacgccac ggcacgaccc ctcacggacc cggcggacgc cgagcacgtc gaggcattgg
     9721 cccgcctccg cgcggaggcg gccgcgttcg tcgcgggtgc gggcgatgcc gtcgacgcgt
     9781 ggcggtcggc cgtcgaggag gacgtcgtgg gggcgctcgc gcggcgatgc cgggacctcg
     9841 cggaattcca cgccggactc gccgacggcc tgccgggccc ggacgacgag cgccggttgg
     9901 cctgggatgc gatccgcgcc tacgccgggg ccacggccag ggaggtctgg gcggtcgtgg
     9961 cggacgaact cgacgccatg gccgtcctgg gcgggctgtc ggaccgttcg gacgacacgc
    10021 tgcgcacgct cgaggaaatg gcggccggcc tgtccgggcc gacggtcgtt cccctcgatc
    10081 ccgcgctctt cgagccgtcg gagggcgacg gcgcgctcac ccggctgcac aagggcctgt
    10141 tccggttccg caggcgcctg cgactcggcc gagcgcggat gcttggcgcg ctcggttccg
    10201 tcttcgggcg ggaccgggtc acgggtcccg cgccggtccg tgcgctctcc ctcgagagcc
    10261 acgcgctggc ccacctgcga ggcaccgtcc ggaacgacct gctctccgcc gaatccgcgg
    10321 cgcgggatgc gctgtcggag gcgctctcgc gggtgcaggt cctggcctcg cagtgggccc
    10381 gggccgtgct gtccgccctg ggccggacgg accggggcgc ctggcatgtc cccggcgaag
    10441 gggtcccggt cgtcgccgta tccccggagg tggacgacga ggcgtggtcg gaagccctgt
    10501 ccgtcctcgg cctggccgcc gagtcggtga ccgcggagct gcgctcgatc gcggatgagg
    10561 tcgatgccgt cgccgtggag ctttccgtgc gaggcggcct ggagacggtc cttctccgcg
    10621 ctctgcgcga ggggggcacc gtgctccacg aggggccgga gcccaggccc gactcgtccg
    10681 caacttcgcg gccgccggtc cgcggatcgg gggaccgctc taggctggcc gccgagatcg
    10741 tcaccttccg ggtcgaactc gcggacgcgg aaaccgggtt ccatcgcgcg gtcgcggagg
    10801 gggtcctcgc accgctcgcc cagtccgggc aggaaatccg ggacatgctc tcgcgcatcg
    10861 gggccgagac gctggagcgc gtcgacgagg gagagggctc ggacgccctg gggcagattc
    10921 acgccgcggc cgtcgaccgg ctcgcgcaga tggtgcgcaa cctcccgcag ccggagcggg
    10981 ccgaggaggt gttccggacc gccgccgagc gcgcgcagtc ggcggtcaca aaccggatcg
    11041 ggacgctgcc ggacgagctc cggcttcccg tcggtcccgg ccggcacgac cgtctcgcgc
    11101 ccatggcatt gcgcagggcg gccgccctgg ccttcgaacg ggacctcgtg ccccggatca
    11161 tggccgccta ccggcccgtc cagcgcgagc tcgtgcggac ctggaacgac agcgagcacc
    11221 tgctccaggt ggtcgattcg accttcggag ccgcgcagga ggaactcgat gccgcgcagg
    11281 gggacgacgc ggcggagcgc cgcgagtccg cccacggcct gatcgaggac ggtctccggc
    11341 gggcggcgga gatcctggag gacggatacg aatcgctggg cgcgccgtgg accggccttg
    11401 ccgagtcggt gcaccgggtg ctccgggacg actggatgca cctggagggt gcggcccagg
    11461 ccgacgactt cctggccaag cactggcagg ggctccgcgc tcggctcaag cgccgggcgg
    11521 agcatgcgct gcggacggcc ggcgaagact gggagagact ctcggggaac gcgatcgtcc
    11581 tgttccgggc ggcccgcatc cggatcgccc gcctcgtccg cctggggcgg gcggcggtcg
    11641 gagtggccga gccacaggcg acggagcgga tccggaccct cgaggcggtg accggcgccg
    11701 cggagcttta cgccggcctt cccctcatct accgcaagct cttcacgctg gacccgctgt
    11761 cggaaaaggg gctcgcggtg aaccggcagg gggatctctc ggccgtcgtc gacgccttcg
    11821 agcgctggaa cggcggaacg ggaccagggc tattcatcgc caattcggag cccgggtcgg
    11881 gcctgacttc cttcctgaac ctggccgcgc gcacactcgc agatcgcgcg cggatcgtgc
    11941 gggtcccgat tccggagcgt gtcgatgacg aatccgtcct cgcccggcat ctcggacgtg
    12001 ccctgggcct gggaacggag ggcccggaga cgctctccga cctggaggag gcgatccaga
    12061 cttccgattt ccagacgggc gtcgtcctgg tggacgacct ggagcacgtg atgctgcaga
    12121 ccccgggcgg aacccgcctc atcgaggacc tgctgctctt cttcgcccga accgatgcca
    12181 gcctcttctg gtgcgcgtcg atcggcgacc tcgcgtggcg attcctggaa cggaccgttc
    12241 ccgcagcgac cggactggcg accggccacg ccctcgattc ctggagccgg aaggcgatcc
    12301 aggcactcat cctggaacgg catcgaagga cgggcatgcc gctccgcttc gaggcgccgg
    12361 acgatcccgc gcccatcctg cgccagcgcc tgcgccgcgc caggacgccc gagcagaccc
    12421 agcgactgct ccgcgacgag ttcttcgacg cgctctttcg ttcggccggc caggaccccg
    12481 cgctcgcgct ctacggctgg gtgcggatgg gacggttcga cgaggaaggc gacgagctca
    12541 ccctgccgcc cttccgcccg ctctccttct cgttcctggg ccacatggac aacgaccgcg
    12601 cgttcgtatt gcgcgcgttc ctgatccacc acaccctctc gcccgaggag ctcgcgcgga
    12661 tcttccggtt ccgaccggac caggcgatgg ccaacctcga gacgctgctc gcgctcgggc
    12721 tcatcgagcc cgtgaccgag ggcgcgcccg tctcgtacgt cgtctccggg atgcggtacc
    12781 ggctccgccg gctggttctg cagcccgtgc gtcgatacct ggccgagcgg cacttcgtgt
    12841 actgagggtc cgggaggtcg cgacggggcc cgcgggcatc acccgatatg catctgcagc
    12901 tcttcgcggc gatgcttctg gcgcagcttc cggagcgcct tctccttgat ctggcgcacg
    12961 cgctcgcgcg tgagcccgaa gcgctggccg atctcctcga gcgtgagcgg atgctcgcgg
    13021 ccgatgccga agtacaggcg ggtgatctcg gcctcgcggg gatgcaggag ggacagcgcg
    13081 cgctcgatgt cgatcttgag ggactcgtcc atgagcgtgt catcgggcga gagatcgtcg
    13141 tcgttcggga gcacgtccag cagcgagttg tcgtcgtcct cgttgaaggg cgcgtccatc
    13201 gacaggtggc ggccggtgtg ctgcatggcc tcgcgcacct tctcgacgtc cacctcgagc
    13261 tcctgggcca gctcctcgat gttcggctgg cgctcgtgca cctgcgccag gcgggcgctg
    13321 gtcttgcgga tcttggagat cgtgccgatc cggttcagcg gaaggcgcac cacgcggctc
    13381 tgctcggcga gagcctgcag gatggcctgg cggatccacc acacggcgta cgagatgaac
    13441 ttgaagccac gcgtctcgtc gaaacgctgc gccgccttga tcaggccgta gttgccctcg
    13501 ttgatgaggt cggccagcgt gagtccctgt ccctggtact tcttcgccac cgacaccacg
    13561 aaccgaagat tggcccgggt cagccgatgc agcgcatcct ggtcgcccgc cttgatgcgg
    13621 cgcgccaggt cgacctcctc ctggggggtc agcagaggaa tctgaccgat ctcctggaga
    13681 tactggtcga gcatccgttg ctggcggggg acgtacataa acctctttcc tcgttgcttg
    13741 gccgaacgcc ggttcagtgg tggacggtga ttctacggtc ccggatctgc tttccggcgc
    13801 gccgcggttc actcgcgcac ggggggtcat gttccccgca aaccgtatga ttcttgtccg
    13861 cttatgatga gggttcggtg attgtttctg caatttcaag caccccgtcc gccgtccgcg
    13921 ggtcctcgct catgcggggc accttggtct gcgcgtcgac gtggtcgcgc gtcgcgcgaa
    13981 gccaccgcag gaacgttcct tcgggcagcg ccacgatctc ggggcgctcg aacgcgccgc
    14041 actcccgccg gattacgtag tgacggttca cccgttgcag gtaggtgtcg atcgccgtcg
    14101 cgatccgttc ggtatccggg gccggaccgg cgaattccac gagccaccgg tggcgcggca
    14161 tccggccctc cacgggaggc acagacgtga cgtggtagtc acggaggtgg gcgccggcgc
    14221 ccgcgcacgc cgattcgagc gccgcgcgcg cctcctctcc gaacacggcc tcgccgtagc
    14281 ggtccagcat ctcgttcgtg cggccggcca cgacgatccg gtgggggcgg gtcccggtga
    14341 accggatcac gtcgccgacg ccgtaggtcc agaggccgga gcaggtcgaa acgcatatcc
    14401 ggtagcggac gtccggctcc acctcggcga gcgtatggcg caggggcgcg tccgacgtgt
    14461 cgtccatccg gacgaactcg tagtagaccc cgctcgccag gtgcagcagc atgtcgcgct
    14521 cgtcgggatg gccctggaac gcgaacgggc cctcggacgc cccgtatccc tccacgaaat
    14581 gcatcggggc cgagccgccg atctgccggc gcatcaggtc ctcgtacgag gagagcgcga
    14641 ccgcgcccga gaagaacacc tcgaggttag gccagacgtc gcgcacgcac gtggcccggc
    14701 cgcccgtcag ctcgttgtac cgctcgacga cgagcggaaa caggaccacg gcccacgacg
    14761 gcaccatcgc catcgcccgg atgtccatgc gacaggtccg gtcggcgatc atccgcagct
    14821 tgcggtccca cccgtcggtg aagagcacgt cccggtgcac ggcctgcctg aaccggcgaa
    14881 tcggccaggg cgccgcgagg tacatgagcc cgctgacctc gcccacccag gtgcccggac
    14941 cgccgacgtc cggctccacg cggcccggca ccgtcagcag gcggccgccc atccagccat
    15001 acttaccggt ggccatcgcg tagcgaaggg ccatgcccag ggtgtagtcg cgtgtccggc
    15061 ggagcatgtc gggcgaaagg gggatgatct tgccggcgct caccgtcccg ctcgacacgg
    15121 cgaaatgccg gatcgaaccg ggccagaaga cgtctttcgc cccctcgcgg actcggcgga
    15181 cgtcgtcccg gtacgcctcg tagtcgtgca gcgggacgcg gtcgcggaac gccgccgtga
    15241 cgcctgccgt gcgggcgatc tccgcgaagc cgaacctccg gccccattcc gtgtcccgcg
    15301 cctcgtggag caggccccgc agcagtcgct cctgcgacgc ggcggggtcc gcgagcgccc
    15361 ggtccagggc gcggatcatg ggcagttgct tcagggccga ggcgacggac atcgagaggt
    15421 atccctgggc gaggcgcatc ctcagacaac gcgaaaaaac cgccggttgt cggccggtgt
    15481 gcgtctcccc gtcgatgaaa cgagaaaccc ggggcgcatc tgacggtgta cgcggttgcc
    15541 gcgagcccgt tctccgtccg catgccggcc ccgcaccgcg atccgacccc cgatcccttc
    15601 gtcctcgact gccgcgggcg gaccgtggac gcgcggccgg gacgggccgt gatcatggga
    15661 atcctcaacg tcacgcccga ttcgttctgg gacggcgggc ggtacgtgga gcccgacgcg
    15721 gcgctccgcc gcgcggtcca gatgctggac gagggggcca cgatcgtgga cgtgggcggc
    15781 gcctcgtcgc gccccgccgg gcgcacgtac ggagagggcg cccgcgcggt gccgccggac
    15841 gaggagacgc tgcgggtggt tccggtcatc gaggccatag cgcgggagcg gcccgacgcc
    15901 gtgatctccg tcgacacggt gcacgcggca gtggcgtcgg ccgcgctgga cgcgggcgcc
    15961 cacctcgtaa acgacgtgac cggcctgcgg aacgaccccg aactggcccg ggtcgcggcg
    16021 ggcgcgggcg cggggctcgt cctcatgcac tccgtcggtg cgtggggcgc gctcgagcag
    16081 gatgtgatgc agggtgacgt cgtggcccgg gtcgccggat cactcgagcg gtcgcgcgac
    16141 ctggcactgg aggccggcgt gcgctcgctg gtgctggatc ccggtttcgg attcgggaaa
    16201 tcgcccgcgc agaaccttcg gctcctcgca cggacgggcg ccttcctcga gctcggccac
    16261 ccggtgctcg ccgccgtgtc gcgcaagagc acgatcggga tcgccctcgg ctcggccgac
    16321 cttccgagac cggtcgccga gcgcctgtac gggtcgctcg gcgccacggc cgtagcggtg
    16381 atgcgcggcg cctcgatcgt ccggacacac gacgttgcgg ccacgcgcga tttcctcgcg
    16441 gtcctcctcg gaactttggc cgccggagac gacggggcgg cgtggagcgg gccttcatga
    16501 tccgtatcat cccggtcaag ccgatgcccg cgtgacgatt ttcccctgga tcataccgat
    16561 tcgcgtcgtc gacctggtcg agatcgccct ggtcgcgtac gtgctgtaca agctgtacct
    16621 gtggatgcgc gggacgatcg cggtgcagat cttcgcgggg atcctggccc tgtacgcgat
    16681 ccaggtgata gtcaccgcgg ccgacatgac catgcttcgg gccatcttcg gatccgtgag
    16741 cgaagtcttc gttctggccg tgatcatcct gttccagccc gagatccggc gcctgctcct
    16801 gatcctcggc cagaaccgcc tcgtacgcgg gttcatgacg gtccccaccc ggaacgaggc
    16861 catcgccgag gtcgcgggcg ccgtcaccga gatgagcaac cgtcgcctgg gcgccctgat
    16921 cgcgatcgag cggtcgaccg ggctccgcag ctacgtcgag acgggcgagc gcctgcaggc
    16981 cgaggtgagc cgcgacctgc tcgtcaccat cttctacgcg cgcaatccgc tgcacgacgg
    17041 ggcggtgatc atccggagcg gacggatcga ggccgcgcgc tgcatcctcc cggtgtccac
    17101 gagcccccgg ctctcgccga acctgggcct gcggcaccgc gccgccgtcg gcctgaccga
    17161 gcagaccgac gccttcgtga tcgtcgtgtc cgaagagacg ggagcgatct cgatcgcgga
    17221 cgggggcacc ctcacccagg gcgttaccag cacccagctc cgggtccgcc tgcaggaggc
    17281 gctctcgtcc ggcccccctg ccccgtccgt cgagcccgcc ccggaggacg tggacgaatg
    17341 acggtcacgc gtccgtacca gcgccgccgc gaggagctcg tcgagatcct gcgcgacaag
    17401 ggcatcaccg acgagcgcgt gctcgaggcc gtccggcgcg tgccgcggca cgaattcgtg
    17461 gaccacgccc tggtgcgccg ggcgtacgag gatgaggccc tgccgatcgg cctcggacag
    17521 accatctcgc agccgttcac ggtcgcgtac cagacggtcc tcctgggcgt gcggcccggc
    17581 gatcgcatcc tggaggtggg tacgggaagc gggtaccagg cggcgatcct gtgcgagctc
    17641 ggcgcgctcg tgttttcgat cgagcgcctg cggccgctct acgtgcggac gcggcacctg
    17701 ctgcgcgacc tgggatacca ggttacgtgc cggctcggcg acgggtcaca ggggtgggag
    17761 gccaagggcc cgttcgacgg gatcgtggtg acggccggcg cgcacaccgt cccggatccc
    17821 ctggtgcagc agctccgcga gccggacgag gactccccgg gcgggcgcct ggtcatcccg
    17881 gtgggcggcc atgcgggcca gacgatgaac cgcatcgtcc ggaccggggc ggagtcgtgg
    17941 gagcgcgagc ggaccgacgg cttccgcttc gtcccgctga tcccggactg agccgctacg
    18001 gctcgtcgcg gccgggggcg gaccacgtgc cgcgctccgt ttccacctgt tccaccacgc
    18061 ccacgatcgc cgcgtccgtc ggcgtcggca gcggtttgaa gggaacggtc gcctcggtcg
    18121 acgtcaccac catcacgtag tcgccgacgc cggagtccac cgtgtcgatg gcgacaaagg
    18181 ggtcgcccgc cggctcgcgg tcgaaggtca ggggctgcac caccatcagt cgccgcccct
    18241 cgaaggacgc caccttgcgg gtcgcccaca cgcttccgat gacgcgcccg aggttcacac
    18301 ggccccctcg gcgtccacgc ggtccacgat ggccgtgatg accgcgtcca cggggcggtt
    18361 gcgggtcagg tcggtctgcc gcgcgctgga gccctgcgcc atgagcacgg tctcgccgat
    18421 gcccgcgccc acgctgtcca cggccacgac gaagcggtcg cgcgacgagc cgtcgagcgc
    18481 aatctcccgg acgatctgga gcgtcaggcc cgtgagctgc tcgtccttgc gcgtcgccca
    18541 gacggtgccg acgacctttc cgaggaccat ggggatcggc cgcgggcggc cggactcgtt
    18601 gcggtggcgc cgccacgtta catcgaacgg cacgcaaatg aaagcgtccg gggccccgga
    18661 acgggtgttc cgggcctcag aacgcgcgcc cttcgcactc gaacggaacg ccgaaatacg
    18721 cggcggccgc ggcggcgtga tcggcgaagg tcgagcgcgt gccgagcggc cgtcccggcc
    18781 ggccgtccca cgagagaagc ggcacgtact cgcgggcgtg gtccgtgccg ggggtcgtcg
    18841 gatcgttgcc gtggtcggcc gtgaggacca gcctcgcgcc ctccgggagg gcctcgacca
    18901 gccccggcag cgcgcggtcg aacgcctcga gcgcccgcgc gaagccctcg gcgtcgttcc
    18961 ggtgcccgaa gtcctggtcg aaatccacga ggttggccca gacgaaggtc gggcggcccc
    19021 ggctcgcacg gatcgctcca tggagtccct cgacgccgtc cgcgttggat ttcgtcttga
    19081 cggtctcgtc gaagccgacc cccgcgaaca ggtcggccac cttgccgacc gacaccgtgc
    19141 ggatcccggc ggccgagagc acggacggca gcgtcggtcc cggcggcggg agcgccaggt
    19201 cgcggcggct ctccgagagc cgcacgaacg ccccgggctc gccctcgaac ggacgggcga
    19261 tcacccggcc gaccgcgtgg cggcccacgc acacccgggt ccgggcggat gcgcaccagt
    19321 cgtagagccg ctcgagcggc acgacgtcca cgtgcgcggc gacctggaag accgagtccg
    19381 cggacgtgta cacgatgggc cggcccgtct cgaggtgcct cgcgccctcc tcgtcgagca
    19441 cggtcgttcc ggaggccggg cggttcgcca gcacggcgtc gacgcccgcc tcgcgacaga
    19501 aggcccggac cacgtcgtcc ggaaagccgt ccgggtaggt cggaaacggc tccatgagcg
    19561 cgagcccggc gagctcccag tggccggtcg tcgaatcctt gccggcggcg gtctcgagca
    19621 tccttcccca ggaggccccg ggcgccccgg cgcacggcac gccgtcgagc gggcggatgc
    19681 atcccaggcc gaacgcggcg aggttcggca gcgccgggcg ccgggcggcg cagacgtgac
    19741 ccagcgtgtc gctctccgcg tcgccgtagc gcgccgcatc gggctgcgcg ccgatcccca
    19801 cgccgtccag gacgatcgtg acgaacaggt gcccggtcgt cactccagga tgacgcactc
    19861 gcccgactcg taggactcgc gcagcgcggc ggtcgcatcc gagcggagct ggtccgggcc
    19921 gtcgtgccgg tcgccgagca tggcgacgcc gacgctcacg ccgccctcga gcagcccgcc
    19981 ctcgccggcc atgcgggcct ggagatcgga cgcccagcgg gccgcgtcat ccacttccac
    20041 gcggcggaac acgccgtagg tgagctcacc gaagcgctcc acgcgcccgt ccgacgtcgc
    20101 gcccttcagc cgctcgtgga agacctcgtc gacctcgcgg agcgtctcgt cgcccgactc
    20161 gccgatcttc tcggcgcggt tcaggtacac gagggcgagg gcgagcggcg cccgtccggt
    20221 gcgggccgcc tccatttcgt cggcgatgat ttcgcgccgc ggtcgcaggg gcacgccggc
    20281 gggggtggaa cgggagggta cggtcatgcc gcgcgacacc agcgaatgca cgagccgacc
    20341 gaattcggcc agcgcgcggg tcgcggccgt ccgttccagg ccggaatcgc ccatcgaatc
    20401 ggcgaccagg agccatgccg cgcggccgtc ctgtccgctc atgggcacga aggccacctg
    20461 gcggatggcg atcgtctcgt ggtagtaccc gagccgtttc ggttcgaagc cgcctggcgc
    20521 cacgcacgac agcacgacgg gctccagggg cttgtgcccg gcgagcaggg gctcggtcac
    20581 ggcgaagcgt cccccgttgc gggcgaaggc gttgcggctg acgaccgctt ccaccgaata
    20641 cgaaagcgct ccctcctcct gccgcaggag gcaaacggtg gtcgcgtcca cggcggcgcg
    20701 caggccccgc aggaccgaga gcatcacctc cccgtcgaac gcgtcggaca cgccctcgac
    20761 caggatccgc gcg
//
LOCUS       DBNHQZ010000348         4783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2947318, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000348 DBNHQZ010000000
VERSION     DBNHQZ010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2947318"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="AC16YU_09430"
     CDS             <1..548
                     /locus_tag="AC16YU_09430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6065488.1"
                     /translation="VDVGRELARIVAEHRGDARRKGLDIWFAEPPETVHARVDPGHFG
                     QAVHQVVDNAVKFTDHGAVAVTLSRMVEGVEVQIRDTGRGIQEEFLPHVFDAFRQESA
                     GTARAYEGTGLGLQIARGLLERMGARIRIESEVGVGTTVLLVLPAADPPARSGPAADR
                     EAEPRRARISPDARSRDRSSA"
     gene            complement(537..2099)
                     /locus_tag="AC16YU_09435"
     CDS             complement(537..2099)
                     /locus_tag="AC16YU_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065489.1"
                     /translation="MDDVPHPQGADPLDPLVVFDRLVVGPVRLERERLVAPYLVESRE
                     GSDATEFVLRFGEPVFEPGDPASENMATMVAAQIALNYGLFAREIVIHGPVDRADRRF
                     IAEMTENTAREIYVNKLLAPNPFLRGTAVGIRPERRGTYSRARLVFPDARSGRARAWP
                     GPAEDRRHVAVLSSGGKDSLLTYGLLRELGYEVHPVFGNESGRHWFTALNAYRHFETT
                     VANTTRVWMNADRVFNWMLRHFPFVRQDFATLRADIYPIRLWTVAVFLFAALPVVRSR
                     GVGRVVIGNEHDTTRRARTHGIPHYDGLFDQSRFFDRALTRYYRQKGMDIAQFSILRP
                     ISELVVQQTLAARYPDLHRHQVSCHAAHADGDRMRPCGRCEKCRRIVGMLSAGGFDPG
                     VCGYTGEQVVHALRSLETRDVHQSGPDAEHLAWLLQERGLIRVDGSSRRRARPHPEVE
                     YLRFDRERSPFDWMPLELRRPLVDLMAEHTRGMVERVSGAWQPLDPSSDRLDLPYPYD
                     AAPPPSGPSSGG"
     gene            complement(2105..3175)
                     /locus_tag="AC16YU_09440"
     CDS             complement(2105..3175)
                     /locus_tag="AC16YU_09440"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN family protein"
                     /protein_id="HIR6065490.1"
                     /translation="MTPLDGGAVVFCDGAFNTPNGKTAHGLVRFTRRYAVLSVIDTRY
                     AGSDAGEVLDRRPRGIPVVADLRAALALPSGREATHFVIGIAPDGGRLTPAMRTAILE
                     AIGMGLDVDCGLHDFLEDDPEISAAAAAAGVRLRDARRTPDRKDLHGFTGKIEEVDAL
                     TIAILGTDSAVGKRTTAWALVLGLEEAGLRAEMVGTGQTAWMQGARFGLLLDSVVSDF
                     MAGEIEHAVWSAWKEARPDVIVIEGQGSLLNPAYPGGNEILAAGRPDLVIMQHAPARV
                     EYDGFPGYRIHPLAEQIEVVERISRKPVIAVTVNHENLDPATLPLVCRTIEQIVGRPT
                     FDVLEHGASGLVEVVRSHLPGR"
     gene            complement(3172..4260)
                     /locus_tag="AC16YU_09445"
     CDS             complement(3172..4260)
                     /locus_tag="AC16YU_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6065491.1"
                     /translation="METEPIPMFDPLLDGATGEPEEMLEVHAPDLRRATDLLLPIRER
                     LEAYEDITESQVGDTSLVRARNLERESGFRQLFLKFEGGNPSGTQKDRIAFAQAADAL
                     RRGFDTVTMATCGNYGVATALACSLAGVRCLVFIPERYRTRRVDEMQRFGAEIVRIEG
                     DYEHAVEASRERAARDELYDANPGGANTTLQLAAYGEIAYEIYDELRDAPAVVAVSVS
                     NGTTLAGIHRGFVSLYRRGRTSRIPRMVAGSAFRKNPIVEAFVRGYDRCVDLVPERIR
                     ETEINEPLVNWHSIDGDPALRSIRQTNGWAHHVSDRSMTSLSRRLRELEGMSVLPAST
                     AGLHALLVAQRQSPLPGDRYVAVLTGKR"
     gene            complement(4260..>4783)
                     /locus_tag="AC16YU_09450"
     CDS             complement(4260..>4783)
                     /locus_tag="AC16YU_09450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6065492.1"
                     /translation="GIANPVALKVGPDMQPSALRALLRVLDPKQEPGRLTLITRFGVN
                     RIEECLPPLVEAVRAEGRTPLWSCDPMHGNTETLGGGVKTRRFENILGELQLAFDIHD
                     ASGSTLGGVHFELTGENVTECLGGASGLAETDLGRAYISQVDPRLNVEQALEMAFSIV
                     KRRRRYGRPRAGP"
BASE COUNT          709 a         1631 c         1719 g          724 t
ORIGIN      
        1 ccgtggatgt gggacgggag ctcgcccgga tcgtggccga acaccgcgga gacgcccggc
       61 gaaaggggct ggacatctgg ttcgccgaac cgcccgagac ggtccatgcg cgcgtcgatc
      121 ccgggcattt cggacaggcc gtgcaccagg tcgtggacaa tgccgtcaag ttcaccgatc
      181 acggggcggt tgcggtcacc ctgtcccgga tggtcgaggg cgtggaggta cagatccggg
      241 acacggggcg gggcatccag gaggaattcc ttccgcacgt gttcgatgcc ttccgtcagg
      301 agtcggccgg cacggcgcgg gcatacgagg gcacgggcct cggcttgcag atcgcccggg
      361 ggctcctgga gcggatgggg gcccgcatcc ggatcgagag cgaagtgggg gtagggacca
      421 cggtcctgct cgtgctgccg gcggcggatc cgcccgcacg ttccggcccg gccgcggacc
      481 gggaggcgga gccgcgccgc gcccggatct cgcccgacgc gcggtcccgc gatcggtcat
      541 ccgcctgacg aaggtccgga gggtgggggg gcggcatcgt acggatacgg gagatccagc
      601 cggtcgctcg acggatccag gggctgccac gcgccgctta cccgctccac catgccccgg
      661 gtgtgctccg ccatgaggtc gacgagcggc cgcctgagct ccagcggcat ccagtcgaag
      721 ggcgaccgct cgcgatcgaa acgcaggtac tcgacctcgg gatggggccg cgccctccgg
      781 cgggacgacc cgtccacgcg aatcagcccg cgctcctgca gcagccaggc caggtgttcc
      841 gcgtcggggc cggactggtg cacgtcgcgg gtctcgaggc tcctcagcgc gtggacgacc
      901 tgctcgccgg tgtaaccgca gacgccggga tcgaacccgc ccgccgacag catcccgacg
      961 attcgacggc acttctcgca ccggccgcac ggccgcatgc gatcaccgtc ggcatgggcc
     1021 gcgtggcagg agacctggtg acggtggagg tcgggatagc gggccgcgag ggtctgctgc
     1081 accacgagct cggaaatggg ccgcaggatc gagaactggg cgatatccat gcccttctgc
     1141 cggtagtacc gcgtcagggc acggtcgaag aaccggctct ggtcgaagag cccgtcgtag
     1201 tgcgggatgc cgtgcgttct cgccctgcgc gtggtgtcgt gctcgttgcc tatcacgacg
     1261 cggcccacgc cccggctgcg gaccacggga agcgcggcga acaggaagac ggcgaccgtc
     1321 cagagccgga tcgggtagat gtctgcccgg agggtggcga agtcctgccg gacgaacggg
     1381 aaatgccgca gcatccagtt gaacacgcgg tcggcgttca tccagacccg cgtcgtgttc
     1441 gcgacggtgg tctcgaagtg ccggtacgcg ttgagcgcgg tgaaccagtg ccggcccgac
     1501 tcgttgccga agaccggatg cacttcgtag cccagctcgc gcagcaggcc gtacgtcagc
     1561 aggctgtcct tcccgcccga cgacaggacg gcgacgtggc ggcgatcctc cgcgggcccg
     1621 ggccacgccc ttgcccggcc gctgcgcgcg tcgggaaaaa cgaggcgtgc ccggctgtag
     1681 gtcccgcggc gctccggccg gatcccgacc gcagttccgc ggaggaaggg gtttggcgcg
     1741 aggagcttgt tcacgtagat ctcgcgcgcc gtgttctcgg tcatctcggc gatgaaccgg
     1801 cggtcggccc tgtccacggg gccgtggatc acgatctccc gcgcgaacag gccgtagttc
     1861 agggcgatct gggcggccac catcgtcgcc atgttctctg acgccgggtc gcccggctcg
     1921 aagaccggct cgccgaaccg taggacgaat tcggtcgcgt ccgaaccctc gcgggactcg
     1981 accaggtacg gtgccacgag ccgctcgcgc tccaggcgga ccggtcccac caccagccgg
     2041 tcaaacacga ccagcggatc gagaggatcg gcgccttggg ggtgcgggac gtcgtccatg
     2101 acggtcatcg gccggggagg tgggaccgca cgacctcgac gagtccggac gcgccatgct
     2161 cgagcacgtc gaacgtgggc cggccgacga tctgctcgat ggtccggcag accagcggaa
     2221 gggtggccgg gtccaggttc tcgtgattga cggtcacagc gatgaccggc ttgcgcgaga
     2281 tccgctcgac gacttcgatc tgctcggcca gcggatggat ccggtacccc ggaaacccgt
     2341 cgtattcgac gcgggccggc gcgtgctgca tgatgacgag gtccggcctg ccggccgcga
     2401 gaatctcgtt gcccccggga tacgccgggt tcagcaggct gccctgtccc tcgatcacga
     2461 tgacgtcggg ccgcgcctcc ttccaggccg accacacggc gtgctcgatc tcgcccgcca
     2521 tgaaatcgct gaccaccgag tcgagcagca gcccgaaccg agcgccctgc atccatgcgg
     2581 tctggcccgt cccgaccatt tccgcgcgca gtccggcctc ctcgaggccg agcaccaggg
     2641 cccaggccgt cgtccgcttg ccgacggcgg agtccgtgcc gaggatcgcg atcgtgagcg
     2701 cgtcgacctc ctcgatcttg cccgtgaatc cgtgcaggtc cttccggtcg ggggtgcgcc
     2761 gggcgtcgcg cagccgtacg ccggcggcgg ccgcggcggc cgagatctcc ggatcgtcct
     2821 ccaggaaatc gtgcaggccg cagtccacgt ccaggcccat gccgatggct tccaggatgg
     2881 cggtccgcat ggccggcgtg agccgtcccc cgtcgggagc gatgccgatc acgaaatgcg
     2941 tggcctcgcg gccggacggc agggccagcg ccgcgcgcag gtcggccacg accgggatgc
     3001 ccctgggccg acggtccagc acctcgccgg cgtcggagcc ggcgtaccgg gtgtcgatca
     3061 cggaaagcac ggcgtagcgc ctggtgaagc gcacgaggcc gtgggcggtc ttgccgttcg
     3121 gggtgttgaa ggcgccgtcg cagaagacca cggcgccgcc gtcgagtgga gtcatcgctt
     3181 tccggtcagc acggcgacat accggtcgcc gggaagtggg ctctgccgct gcgcgaccag
     3241 gagggcgtgc aggccggccg tcgaggccgg gagcacgctc atgccctcga gctcgcgcag
     3301 cctccgggag agcgacgtca tggaccggtc cgacacgtgg tgcgcccagc cgttcgtctg
     3361 ccggatgctc cggagcgcgg ggtccccgtc gatcgagtgc cagttgacga gcggctcgtt
     3421 gatctcggtc tcgcggatgc gctcggggac caggtccacg cagcggtcgt atccgcggac
     3481 gaaggcctcg acgatcgggt tcttccggaa cgcggaaccg gcgaccatgc gggggatgcg
     3541 ggacgttcga ccgcgccggt acagggacac gaagccgcgg tggatgccgg ccagcgtcgt
     3601 tccgttcgac acggacaccg cgacgaccgc gggcgcgtcc cgcagctcgt cgtagatttc
     3661 gtaggcgatc tcgccgtagg cggccagctg cagggtggtg ttcgcgccgc ccggattggc
     3721 gtcgtagagc tcgtcgcggg cggcccgttc cctggaggcc tccaccgcgt gctcgtaatc
     3781 cccctcgatg cggacgatct ccgccccgaa ccgctgcatc tcgtccacgc ggcgcgtccg
     3841 gtaccgctcg gggatgaata ccaggcaccg gacgccggcc aggctgcagg cgagcgccgt
     3901 ggccacgccg tagttgccgc aggtcgccat cgtgacggtg tcgaagccgc gccgaagggc
     3961 gtcggccgcc tgcgcgaacg cgatgcgatc cttctgcgtg ccgctcggat tgccgccctc
     4021 gaacttcagg aagagctgcc ggaatccgct ctcccgctcc aggttgcggg cgcgaacaag
     4081 gctcgtgtcg ccgacctggg actccgtgat gtcctcgtag gcctcgagcc gctcacggat
     4141 cggcagcagg aggtcggtcg ctcgacggag gtcgggggcg tggacctcca gcatttcttc
     4201 gggttcaccg gtggccccgt ctagcagcgg gtcgaacatg gggattggtt ctgtctccat
     4261 cagggtccgg cgcggggccg gccgtatcga cgtcgtcgtt tgacaatgga aaaggccatt
     4321 tccagcgcct gctccacgtt caggcgcgga tcgacctggc tgatgtaggc ccggcccagg
     4381 tcggtctcgg cgaggccgct cgccccgccg aggcattcgg tcacgttctc gccggtaagc
     4441 tcgaaatgca cgccgccgag cgtggacccg gaggcatcgt gaatgtcgaa tgccagctgc
     4501 agctctccca ggatgttctc gaaacgccgg gtcttgaccc cgccgccgag cgtctccgtg
     4561 tttccgtgca tgggatcgca gctccacagc ggcgtgcgac cctccgcacg gaccgcctcg
     4621 acgaggggcg gaaggcactc ctcgatccgg ttgacgccga accgggtgat gagggtcaga
     4681 cggcccggtt cctgcttcgg gtccagcacg cgcaagaggg cacgcagcgc cgagggctgc
     4741 atgtcgggcc cgaccttgag ggcgacggga ttcgcgatgc cgc
//
LOCUS       DBNHQZ010000349         8028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2407238, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000349 DBNHQZ010000000
VERSION     DBNHQZ010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2407238"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="AC16YU_09455"
     CDS             <1..1591
                     /locus_tag="AC16YU_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065493.1"
                     /translation="VWELLPLAQIDPLHVRTVSLGRPDVVRADLRRSFPARPLTEMRY
                     RSAGGGLQAVDVTHAQRRTLGRGTRAGVVFGYGGAGARGEYDGSRLRRKRRLFIGLEL
                     SRADWSVAVTNLHNRNRVGAHGGVLPIPGLEYDSIYNRLLADVEDPSAIRIRIRNDLA
                     LALSGPVGGSPLTVRAYRTNATLQYRGPADTTTARMERIGAVAEWPVRAVRIPVVVRA
                     SAFTESVRSGDAFATPPGRTFTADASIAATPALGDWTVQALAGAGSLAGDAGASGHLA
                     ASGPLGPVRVHGSASLAHVPRSAAERHGFGPAFLGAPGAGASRTVRFEGGLEVRSGVF
                     RVGAAAFARSVADPVHLLLDASGDTVTTVVGGDDLRTRGVILSLGLRDRRPRGLYLEG
                     SGTWNPRSDTGGELAARASESVPELHGRIRLGARALLFGGDLDLDLAVQARAWSRTAG
                     LRLHAPTGLLALPDPAARELPGSAIVDVTVDAGVRSATVFLAFENLLSGTTLVPGNQL
                     IPDYPYPERRFRFGVYWPIFD"
     gene            1660..2505
                     /locus_tag="AC16YU_09460"
     CDS             1660..2505
                     /locus_tag="AC16YU_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HIR6065494.1"
                     /translation="MTNTPLQPSDYEFRALDGIDAWRNPPASWYRSMRGQLDRAWDSA
                     TDWAKRVPGVEWAIENIVTGLLELTNEISQDSVWRDGVLRDFVRAGSTVRSLEEIRTL
                     DLSVVDRALDGLELKYKSLAGAEGAASGLAGAVGIVPDIVALVAINLRAAGEYATYCG
                     FDVTSREERLYALQILDLVAGPGDHPKHIAFEPAVRMAKRVAQKQGIQAVEQMGFNEA
                     VERLARAIGIHLTGRKLGQVVPVAGAAVGAGLNVLYTGKVCRAAFHLYRERFLVGKYG
                     LEPAI"
     gene            complement(2555..3517)
                     /locus_tag="AC16YU_09465"
     CDS             complement(2555..3517)
                     /locus_tag="AC16YU_09465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR6065495.1"
                     /translation="MATPPAIPELFWERLDAIVPADRLDGVRASFSAPRHGSGRVNRL
                     RAEAEEMLGELRAAGLHPRALDWYPDGFTVDPDEREALGRTAAAGDGRIWLQNPASMV
                     PPLALGPEPGERVLDLAAAPGSKTLQIAALMRGEGELAAVEVVRDRFFRLQRNLEQGG
                     ASFARTFLKDGSRVWRHRPAYFDRVMVDAPCTTEGRFRVDEPGTFAYWSPRKIREMVR
                     RQVPLLQGALRAVRPGGIVVYSTCSFAPEENELVVDRVLAWAGDAVQTEALPLELPRM
                     APALEGWEGRAVDPGVRAARRILPDGIFEGFFVCRFRRLGPLAD"
     gene            3710..4984
                     /gene="clpX"
                     /locus_tag="AC16YU_09470"
     CDS             3710..4984
                     /gene="clpX"
                     /locus_tag="AC16YU_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR6065496.1"
                     /translation="MGNRRGEDVIRCSFCGRTAHEVTSMVAGPDVYICDRCINDAAGI
                     VRTDLTTHRPAEAAPQPRRATPRRRLTPVEIKQALDEYVIGQDQAKKALSVAVYNHYK
                     RIDGTGYVPDFEEVELEKSNILLLGATGTGKTLLARTLARILDVPFSISDATALTEAG
                     YVGEDVESILAHLLHAADFNLERAERGIIYIDEIDKIARKSDNASITRDVSGEGVQQA
                     LLKILEGTIAGVPPKGGRKHPEQSLINIDTSNILFICGGAFDGLGDIISHRLSTNSIG
                     FTADTHRRFDKSDPTILQRVEPDDLLRFGLIPELIGRLPVMSALDALSADAMLDILLR
                     PRNALVKQYQKLFAMDGIDLVFDDDALQAVVDRASALGTGARGLRSVLESVMLEIMFG
                     VHGMSDVSSVRITADTVVHGLEPVYELRKASA"
     gene            5142..6164
                     /locus_tag="AC16YU_09475"
     CDS             5142..6164
                     /locus_tag="AC16YU_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR6065497.1"
                     /translation="MLSSLRYYLRGLFRQIYEKDVFLWAQAIAFKVLITLVPIVILGT
                     GLFGRVLRQERPFASVERVIRDFVPAYGADRLIGFLGQLQGASTALTIIGVATLVVTV
                     MTLFTTLRIVLANIFREDWHTSRSLFRGYLFDIRMALQAGLFFVLSIGLTVLVQMMGP
                     AGLSALSGIGAGTGWLQALWDTAVHGLGVLLPFLMSVGMFFQLLWFTPVPRPPKRSAL
                     AGAVVGALLWEAAKFAFTAYATHMGGFDEGFESMLGDTFLLVLMLLFWAYYSGLVLCV
                     GAIVTLLYEARQRLRLWAAETATAADTAAPGSAASGPGPDLPGAAESASRPVSGSVPQ
                     HSESHD"
     gene            6157..>8028
                     /locus_tag="AC16YU_09480"
     CDS             6157..>8028
                     /locus_tag="AC16YU_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR6065498.1"
                     /translation="MTERAATLLIVGLVTLVAAGPARAQDPLVLVNDSTQVRSVRFRF
                     VDGHLFDAERLANEMVTRGPGFFGRLRRRLAFLPLISAPDLPFDPIELQRDMERLRRF
                     YRRNGFLQPSIDYPVSQFDASSNRIGVIVTVREGPPLTVRNSEVRLVDPLPSAMVEGW
                     DRMVGRLGIREGERYTEFERLAAEGRVLNWMSRRGYAFVNVVGSVAVDSVRFLADVEL
                     RVTPGPRATIDSVLVEGAESVSDRVILRQVPFEPGKRYDAGDLEDGQRALFGLNLFRV
                     ALVDVPPQPADSTVTVRIRVREAKARLLSTETGFSLENGLDMDATLRHRNFLGDARQF
                     SFSVAYRPGWFARPPEGRSAVRTFSLASSLQQPYIGIRKMSGSATVFYRWIDDPNQDT
                     RYREIGVTPSLIYEFLPYRTAIVEFGASRAIPIRTAGPLSAIGVYDRSTFSSSVRLGK
                     LNDYLHPRRGWLANPRVEVGGLLFGQLAYTKGTLDATGLIPITLHSSVVVTATVGRLV
                     PRGASRNQADPENEYRFDAIRYYAGGSSDVRGWDLQTLGPKFARADSIVTEADGERRA
                     VGAAYESVGGLGKISFSLELRVPAPGLGSSWQLGAFLDGGAIPARIRTDDAGRTLFDA
                     "
BASE COUNT         1126 a         2904 c         2761 g         1237 t
ORIGIN      
        1 cgtctgggag ctcctgccgc tcgcccagat cgatcccctc cacgtccgga cggtgtctct
       61 gggccggccg gacgtggtcc gcgccgacct gcggcgctcc ttcccggccc gtcccctcac
      121 ggagatgcgg taccgctcgg cggggggcgg cctgcaggcc gtggacgtca cccacgcgca
      181 gcgccggacg ctcggccgcg ggacgcgggc cggtgtcgtc ttcggctacg gcggcgccgg
      241 cgcccgcggc gagtacgacg gcagccggct tcggcggaag cggaggctct tcattggcct
      301 cgagctctcg cgcgccgact ggtcggtcgc agtcacgaac ctgcataacc ggaaccgcgt
      361 gggcgcccac ggcggcgtgc ttccgatccc gggcctggag tacgactcca tctacaaccg
      421 gctgcttgcg gacgtcgagg atccctcggc catacggatc cgcatccgca acgacctggc
      481 gctcgccctc tcgggcccgg tcggagggtc gccgctcacg gtccgggcct accggacgaa
      541 cgccacgctg cagtaccgcg gcccggccga taccacgacc gcccggatgg agcggatcgg
      601 cgccgtcgcc gaatggccgg tccgagccgt ccggattccg gtggtcgtgc gcgcctccgc
      661 ctttaccgag tccgtccgct ccggcgacgc cttcgccacg ccgccggggc ggacgttcac
      721 ggccgacgcg tcgatcgccg ccacgccggc gctcggcgac tggacggtcc aggcgctcgc
      781 cggagcgggc agcctcgcgg gcgacgccgg agcgtccggt cacctggctg cgtccggccc
      841 gctgggcccg gtacgcgtgc acggatcggc ctccctcgcg cacgtgccgc ggtcggcggc
      901 ggagcgccac ggattcggtc ccgccttcct cggggcgccc ggggccggcg cgtcgcgaac
      961 cgtacgcttc gagggcggtc tcgaggtcag gtcgggcgtg ttcagggtgg gtgccgcggc
     1021 cttcgcgcgc tccgtggccg acccggtgca cctgctgctc gatgcgtccg gagacaccgt
     1081 cacgaccgtg gtgggcgggg acgacctccg gacgcggggc gtgatcctct ccctcggcct
     1141 gcgcgaccgg cgcccgcgcg gcctgtacct ggaggggtcg gggacctgga atccccgctc
     1201 cgacacgggc ggagagctcg ccgcgcgggc ctccgagtcc gtccccgagc tgcacggccg
     1261 gatacgcctc ggcgcccggg ccctcctctt cggaggcgac ctggacctgg acctcgccgt
     1321 gcaggcccgc gcctggtccc ggacggccgg gctccggctc cacgctccga ccgggctcct
     1381 cgccctgccc gaccccgcgg cgcgcgagct ccccggatcg gcgatcgtgg acgtcacggt
     1441 ggatgccggc gtccggtcgg ccacggtatt cctcgcgttc gagaacctct tgagcgggac
     1501 gacgctcgtg cccggcaacc agctcattcc ggactacccg tatcccgagc gccgattccg
     1561 attcggcgtg tactggccca ttttcgactg agggccgccc gtggagccgc gacgaacctt
     1621 ccggcgttcg tccgtatacg ggatcgatcc gaaatcgccg tgacgaacac cccgctccag
     1681 ccatccgact acgagttccg cgcgctcgac ggcatcgacg cgtggaggaa tccgcccgcg
     1741 tcgtggtacc ggtctatgcg cggccagctc gatcgggcct gggactcggc gacggactgg
     1801 gcaaagaggg ttccgggcgt cgaatgggcc atcgagaaca tcgtcacagg actcctggag
     1861 ctcacgaacg agatcagcca ggactcggtg tggcgggacg gcgtcctgcg cgacttcgtg
     1921 cgcgcgggca gcacggtgcg gtcgctcgaa gagatccgca cgctcgacct gtccgtcgtg
     1981 gaccgcgccc tcgacggcct ggagctcaag tacaagtcgc tggccggcgc ggagggcgcg
     2041 gcctcgggcc tggccggggc ggtcggcatc gtgccggaca tcgtggcgct cgtcgccatc
     2101 aacctgcggg cggcgggcga gtacgcgacc tactgcggct tcgacgtgac gagccgggaa
     2161 gagcgcctct acgccctcca gatcctggac ctcgtggccg gtccgggcga ccacccgaag
     2221 cacatcgcgt tcgagcccgc ggtccgcatg gcgaagcggg tcgcgcagaa acagggcatc
     2281 caggccgtgg agcagatggg gttcaacgag gcggtcgagc ggctggcccg ggcgatcggc
     2341 atccacctca cggggcgcaa gctgggccag gtcgtgcctg tggccggggc ggccgtcgga
     2401 gccggcctga acgtcctcta cacgggcaag gtgtgccggg cggccttcca cctgtaccgt
     2461 gaacggttcc tcgtgggcaa gtacgggctg gaaccggcga tctgagcggt ccgggtatcc
     2521 cgaaccgcac gaaaaggccg ggcctcggat cgtctcagtc cgccagcggc ccgagccgac
     2581 ggaaccggca cacgaagaac ccctcgaaga tgccgtcggg cagtatccgc cgggccgcgc
     2641 ggaccccagg gtcgaccgcc cgaccctccc agccctcgag ggcgggagcc atccggggca
     2701 gctcgagcgg aagcgcctcc gtctggaccg cgtcgccggc ccacgcgagg acgcgatcca
     2761 ccaccagctc gttctcctcc ggcgcgaacg agcacgtgga gtacaccacg atcccgcccg
     2821 gccggaccgc gcgcagggcg ccctgcagca gcggcacctg ccgccggacc atctctcgga
     2881 tcttgcgcgg gctccagtac gcgaacgtcc ccggctcgtc cacacggaac ctgccttccg
     2941 tggtgcacgg cgcgtccacc atcacgcggt cgaaatacgc cggccggtgg cgccatacgc
     3001 gcgagccgtc cttcaggaag gtgcgcgcga acgaggcgcc gccctgctcc aggttccgct
     3061 gcaggcggaa gaagcggtcc cgaacgacct ccacggccgc cagttcgccc tcgccccgca
     3121 tcagcgcggc gatctgcagg gtcttgctgc ccggagctgc cgccaggtcg agcacccgtt
     3181 cgcccggctc cgggccgagc gcgagagggg gcaccatgga cgccgggttc tgcagccaga
     3241 tccgcccgtc gcccgcggcc gcggttcgcc cgagcgcctc ccgctcgtcc gggtcgaccg
     3301 tgaatccgtc ggggtaccag tcgagggcgc ggggatggag gccggcggcc cggagttcgc
     3361 cgagcatctc ttcggcctcg gcgcgcagcc gattcacccg cccggacccg tggcgagggg
     3421 ccgagaagct ggcccgtacg ccgtcgagcc ggtccgccgg gacgatggcg tcgaggcgct
     3481 cccagaagag ctcgggtatc gcggggggcg tggccatgcg tgccgaatcc ccgcgcccgg
     3541 acgcggttcc gcccgcccgg cggacggcca tcggaaaccg ccgtttctga cgccggaacg
     3601 gcccgttcgc cgtttaagca gttgccggct ttgcgcgata tatgtaccat gatcgagccc
     3661 ggcacgaggc cgggcccggg catccgaatc tcgagaccga gcgggcgaca tggggaaccg
     3721 acgcggcgag gacgtcatcc gctgcagctt ctgcggcagg acggcccacg aggtgacctc
     3781 catggtcgcc ggcccagacg tctatatctg cgaccgctgc atcaacgacg cggccgggat
     3841 cgtccggacc gatctcacga cgcaccggcc cgcggaagcc gctccgcagc cccggcgcgc
     3901 cacgcccagg cgccggctca cgccggtcga gatcaagcag gcgctggacg agtacgtgat
     3961 cggccaggac caggccaaga aggcgctctc ggttgccgtc tacaaccact acaagcggat
     4021 cgacggcacg gggtacgttc ccgacttcga ggaggtggag ctcgagaaat cgaacatcct
     4081 ccttctcggc gcgacgggca ccgggaagac gctcctcgcg aggaccctcg cccgcatcct
     4141 ggacgtgccc ttctcgatct cggacgccac ggcgctgacc gaggcgggct acgtgggcga
     4201 ggacgtcgag agcatcctgg cgcacctgct ccacgcggcc gacttcaacc tggagcgggc
     4261 cgagcgcggg atcatctaca tcgacgagat cgacaagatc gcccgcaaga gcgacaacgc
     4321 gtccatcacg cgcgacgtgt cgggcgaggg cgtacagcag gcgctgctca agattctcga
     4381 gggtacgatc gccggcgttc cgccgaaggg cggtcgcaag cacccggagc agagcctgat
     4441 caacatcgac acgtcgaaca tcctcttcat ctgcggcggc gcgttcgacg ggctggggga
     4501 catcatctcc caccgcctgt ccacgaactc catcggcttt acggccgaca cgcaccgccg
     4561 cttcgacaag agcgatccga cgatcctgca gcgcgtggaa ccggacgacc tgctccggtt
     4621 cggcctgatc ccggaactca tcgggcgcct tcccgtgatg tccgccctgg acgcgctttc
     4681 cgccgatgcc atgctggaca tcctgctgcg ccccaggaac gcgctcgtga agcagtacca
     4741 gaagctcttc gcgatggacg gcatcgacct cgtcttcgat gacgacgcgc tccaggccgt
     4801 cgtcgaccgg gcgagcgccc tgggcacggg ggcgcgcggc ctgcgctccg tgctcgagag
     4861 cgtgatgctc gaaatcatgt tcggcgtgca cggaatgtcc gacgtctcgt cggtccgcat
     4921 cacggccgat acggtggtgc acggcctcga gccggtgtac gaactccgga aggcctccgc
     4981 ctgaggccgg acgctccccg cggagtcgac cctcgccgga atccggcggg ggttttccca
     5041 tttcgccccc ccgggatccc gcaggtcgcg ctattcaccc ggaaatgcgt gcaacttacc
     5101 gggatcggcg tgtaccatgc agggaattgg ctccggtgcc gatgctctcg tcccttcgtt
     5161 attacctacg cggccttttc cggcagatct acgagaagga cgtcttcctg tgggcccagg
     5221 cgatcgcatt caaggtgctg atcacgctgg tcccgatcgt cattctcggg accggcctgt
     5281 tcgggcgggt cctgcggcag gaacgcccgt tcgcctccgt ggagcgggtg atccgcgatt
     5341 tcgtgcccgc gtacggagcg gatcgcctga tcggattcct cggacagctc cagggggcca
     5401 gcacggcgct gaccatcatc ggcgtggcca cgctcgtcgt gaccgtcatg acgctcttca
     5461 ccacgctgcg gatcgtcctc gcgaacatct tccgcgagga ctggcatacg tcgcgcagcc
     5521 tgttccgcgg gtacctattc gacattcgca tggcgctcca ggcggggctc ttcttcgtgc
     5581 tgtcgatcgg cctgaccgta ctggtccaga tgatggggcc cgccggactg tccgcgctgt
     5641 ccggcatcgg tgccggaacg ggctggctcc aggcgctgtg ggataccgcc gtgcacggcc
     5701 tgggagtcct gctgccgttc ctgatgtcgg tcgggatgtt tttccagctc ctgtggttca
     5761 cgcccgtccc gaggcctccc aaacgaagcg ccctggccgg agcggtggtc ggcgccctgc
     5821 tctgggaggc ggccaaattc gccttcaccg cgtacgccac ccacatggga ggcttcgacg
     5881 agggcttcga gtcgatgctc ggggacacct tcctgctcgt gctcatgctg ctcttctggg
     5941 catactactc gggcctggtc ctctgcgtcg gggcgatcgt cactctgctc tacgaggcca
     6001 ggcagaggct ccggctctgg gcggccgaga cggcgacggc ggcggacacc gcggcgcccg
     6061 gttccgcggc ctccggaccc ggtccggacc ttcccggcgc agccgaaagc gcgtcgcggc
     6121 cggtcagcgg gtccgttcca cagcattcgg agagccatga ctgagcgcgc ggcaaccctg
     6181 ctgatcgtgg ggctcgtcac gctcgtcgcg gccgggccgg cgagggcaca ggacccgctc
     6241 gtcctcgtga acgactccac gcaggtccgt tccgtccgct tccgcttcgt ggacgggcac
     6301 ctgttcgatg ccgagcgtct cgcgaacgag atggtcactc ggggcccggg tttcttcggc
     6361 aggctgcgcc gacggctcgc cttcctgccg ctgatctccg cgcccgacct gccgttcgac
     6421 ccgatcgaac tgcagcgcga catggagcgg ctgcgccggt tctaccgcag gaacggcttc
     6481 cttcagccgt cgatcgacta cccggtgagc cagttcgatg cctcgtccaa ccggatcggc
     6541 gtcatcgtca ccgtccggga ggggccgccg ctgacggtcc ggaacagcga ggtccggctc
     6601 gtggacccgc tgccttcggc catggtcgag ggatgggacc gcatggtggg gcgcctcggc
     6661 atccgcgagg gcgaacgcta cacggagttc gagcggctcg ccgccgaggg gcgagtcctg
     6721 aactggatga gcaggcgggg ctatgcgttc gtgaacgtgg tgggttcggt cgcggtcgac
     6781 tcggtccgtt tcctggcgga cgtcgagctg cgcgtcacgc ccggcccgcg ggcgacgatc
     6841 gattcggtcc tcgtcgaggg ggccgagtcc gtgtccgacc gggtgatcct gcggcaggtg
     6901 cccttcgaac ccgggaagag gtacgatgcg ggcgacctgg aggacgggca gcgcgccctc
     6961 ttcggcctga atctcttccg cgtcgcgctc gtggacgtgc ctccccagcc ggccgacagc
     7021 acggtgacgg tccgcatccg cgtgcgcgag gcgaaggccc gcctgctctc gaccgagacc
     7081 ggcttctcgc tcgagaacgg cctggacatg gatgccaccc tccggcaccg caacttcctc
     7141 ggcgacgccc ggcagttctc gttctcggtc gcgtaccggc ccggctggtt cgcccggccg
     7201 ccggaggggc gttcggccgt gcgcacgttt tcgctggcgt cgtccctgca gcagccctac
     7261 atcgggatcc ggaagatgtc gggctcggcc actgtcttct accggtggat cgacgacccc
     7321 aaccaggaca cccggtaccg cgagatcggc gtgaccccgt cgctcatcta cgagttcctc
     7381 ccctaccgga cggcgatcgt ggagttcggg gcgagccgcg ccatcccgat ccggaccgcc
     7441 ggcccgctgt cggccatcgg ggtatacgac cgttccacct tttcgtcgtc ggtgcggctc
     7501 ggcaagctga acgactacct gcaccctcgg cgcggctggc tggcgaaccc gcgcgtggag
     7561 gtgggcggac tcctcttcgg tcagctcgcc tacaccaagg gcaccctgga cgccacgggc
     7621 ctcatcccga tcacgctcca ctccagcgtc gtggtcacgg cgaccgtggg ccgcctcgtc
     7681 ccgcgcgggg ccagccggaa ccaggccgat cccgagaacg agtaccggtt cgacgccatc
     7741 cgctactacg cgggcggatc gagcgacgtc cggggatggg acctgcagac gctcggtccg
     7801 aaattcgccc gcgcggactc gatcgtgacc gaagccgacg gagagagacg ggccgtcggc
     7861 gccgcgtacg agtccgtcgg cgggctcggc aagatctcct tcagcctgga actgcgcgtg
     7921 ccggcccccg gtctcggctc gagctggcag ctcggcgcct tcctggacgg cggcgccatt
     7981 ccggcccgga tccgcacgga cgacgcgggg cgcacgctct tcgacgcg
//
LOCUS       DBNHQZ010000350         3133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2269890, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000350 DBNHQZ010000000
VERSION     DBNHQZ010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2269890"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1306)
                     /locus_tag="AC16YU_09485"
     CDS             complement(170..1306)
                     /locus_tag="AC16YU_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6065499.1"
                     /translation="MTLSDDTIRLLLDDGPWTAGRLAELERAAGSASEARRLVGRWRS
                     IEAAFADRVSDRIPDPGDLVLLGIERAGRGQDLSDSERARLDAARGRLSDAEAAIPAL
                     EDILSSIGRDATAFDAAWRSAFSDRPARLDRPARRPVPLRRVVSRFAMAASVLAFAAV
                     SVLLLQRDRQTVTMTADDAVRLVTLDDGSTVRLLRGASLSWHGGDFDRRLTLEGRAYF
                     NATPDEVPFQVLTPSATVTVLGTSFGVDSGPDETRVVLVSGRVAVASAGDPGAMIVLD
                     PGQRTRVVRGSPPTEPESVDAEAALSWTGLLFFRSTPLSEVASRLSAAYGVRIVVDPA
                     LTAESVTGTFERDRASGEVIGIVATAVGARVERDADGTLRLLPR"
     gene            complement(1404..1964)
                     /locus_tag="AC16YU_09490"
     CDS             complement(1404..1964)
                     /locus_tag="AC16YU_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6065500.1"
                     /translation="MSAAGNAIETLVLRAREGDRSARSTLLERLQPMLRGYFVRRIGL
                     RDEVDDLVQNTLLRVDRGLNDLRDPAKLRPFTLKAALFELQDLYRGRYSVREVPLSDD
                     LPPPSTDPAPTGLAIDLDRALGALTPRARRIMELKLMGYRYEEIAGMIDSTEAAVKMQ
                     VKRAVERLRDLLAVILWLTLQGGLRP"
     gene            complement(1968..2840)
                     /gene="pheA"
                     /locus_tag="AC16YU_09495"
     CDS             complement(1968..2840)
                     /gene="pheA"
                     /locus_tag="AC16YU_09495"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR6065501.1"
                     /translation="MTVTFQGEIGAYSEEAALAFVPGADLRPLPSFEAVFEALVDGTA
                     DLAVAPIENSLHGSVHTNYDLLREHDVRIVGEVQVRVRHHLMAVAGTTVESVREVHSH
                     PQALGQCRDFLRTRLPRALVVPSYDTAGAAKMVAERAEPGVAAIASDRAAVEYGLEVI
                     ADGIESNRLNFTRFLALVRSDAPRALPAPTRPGAGMKTSVLYVPHENIPGVLFRSLAV
                     FALRDIDLFKIESRPLVGSPGQYIFYLDLAGSVDQEPVTKALDHLREIAAYVKVLGSY
                     PRDHPAAGTAPETD"
     gene            complement(2845..>3133)
                     /locus_tag="AC16YU_09500"
     CDS             complement(2845..>3133)
                     /locus_tag="AC16YU_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR6065502.1"
                     /translation="EADLSLLDHETELALVKVLLGFPEAIVRAAEAREPHRVAVYLRD
                     VAVAFTQFYGASRIIGQPPDIASARMALALAVRIVLRNGLAVLGISAPDRM"
BASE COUNT          478 a         1091 c         1167 g          397 t
ORIGIN      
        1 gaggagggcg acgaagaggg ggagggacgg tcggcgcacc gggtccttgg agcgggacat
       61 ccctcgaaga tagacgggag gggccgcggc tcacaaccgc gcgggcggtc gcgctccgca
      121 ccgcgccggg gtccggcacc gcgcacctgc cgccggccga tgcgtccgtt cagcggggaa
      181 ggagacggag cgttccgtcc gcgtcccgtt cgacccgcgc gccgacggcg gtcgcgacga
      241 tgccgatcac ttcgcccgac gcacggtcac gctcgaacgt gccggtcacc gactcggcgg
      301 tgagcgccgg gtcgaccacg atccggacgc cgtacgcggc ggagagccgc gaggcgacct
      361 cggacagcgg ggtggagcgg aagaacagga gccccgtcca ggacagggcg gcctcggcat
      421 ccaccgactc gggctccgtg ggaggggagc cgcggacgac acgggtgcgc tggcccggat
      481 ccaggacgat catggccccg ggatcgccgg cggacgccac cgcgacccgg ccggagacca
      541 ggacgacccg ggtctcgtcg ggaccggaat ccacgccgaa ggacgtgccg agcaccgtga
      601 cggtcgcgct cggcgtgagc acctggaagg gcacctcgtc cggcgtcgca ttgaagtagg
      661 cgcggccctc gagggtgagc cgacggtcga agtcgccgcc atgccacgac aggctggcgc
      721 cgcggagcag ccggaccgtc gatccgtcgt caagggtcac gaggcggacg gcgtcgtccg
      781 cggtcatcgt cacggtctgc cggtcccgct gcaggagcag gaccgaaacc gccgcgaacg
      841 ccagcaccga ggccgccatg gcgaagcggg acacgacgcg gcgaagaggc accgggcgac
      901 gggccggacg gtccagccgg gccggacggt cgctgaacgc ggagcgccag gccgcgtcga
      961 acgcggtcgc gtcgcggccg atcgacgaga ggatatcctc gagggcagga atcgccgctt
     1021 cggcgtccga gagcctgccg cgcgccgcat cgagtcgggc gcgctccgag tccgacaggt
     1081 cctgaccgcg gccggcgcgc tcgattccca ggagcaccag gtcgcccggg tcggggatcc
     1141 gatccgagac ccggtccgcg aaggcggcct cgatcgaccg ccagcgcccg acgagccgcc
     1201 tcgcctccga ggcggagccg gccgcccgct cgagttcggc gagccgcccg gcggtccagg
     1261 gaccgtcgtc cagcaggagt cggatggtgt cgtcagatag ggtcatcggc gggtcatccg
     1321 gaacggatgc acgggggcgc acgcgcgggt cgtggtcccg tgtacaggta gccgccgcga
     1381 ccggcgacaa ggtaacgtcg ccctcacggc cggagccccc cctgcagcgt cagccacagg
     1441 atgacggcca gcaggtcccg gaggcgctcc acggcccgct tcacctgcat tttcacggcc
     1501 gcttcggtcg aatcgatcat gccggcgatc tcctcgtagc ggtatcccat gagcttgagc
     1561 tccatgatcc gccgcgcgcg gggggtcagg gccccgagcg cgcggtccag gtcgatcgcg
     1621 agccccgtcg gtgccgggtc ggtggacggc ggcggcaggt cgtccgagag cgggacctcg
     1681 cgcacggaat accggccgcg gtacaggtcc tggagctcga agagcgctgc cttcagcgtg
     1741 aacggccgga gcttcgcggg atcccgcaga tcgttcaggc cccggtccac gcggaggagc
     1801 gtgttctgga ccaggtcgtc cacctcgtcc cgcaggccga tccggcgaac gaaatacccg
     1861 cggagcatcg gctgcagccg ctcgagcagg gtcgaccgcg ccgagcgatc cccctccctc
     1921 gcgcggagga ccagcgtctc gattgcgttc ccggcagccg acatcggtca gtccgtttcc
     1981 ggagccgttc cggcggcggg atggtcgcgg ggatacgagc cgagcacctt cacgtacgcg
     2041 gcgatctcgc gcaggtgatc cagggccttc gtcaccggct cctggtcgac ggagccggcc
     2101 aggtccaggt agaagatgta ctgccccggg cttccgacca gggggcggct ctcgatcttg
     2161 aacaggtcga tgtcgcggag ggcgaacacc gccaggctcc tgaacagtac cccggggatg
     2221 ttctcgtggg gcacgtacag gacgctcgtc ttcatgccgg cgccgggccg cgtgggcgcc
     2281 gggagggcac gcggggcgtc cgaccggacg agcgcgagga atcgcgtgaa attgagccgg
     2341 ttgctctcga tgccgtcggc gatcacctcg agcccgtact cgacggccgc gcgatcgctc
     2401 gcgatggcgg ccacacccgg ctccgcccgt tcggccacca tcttcgcggc cccggccgtg
     2461 tcgtaggacg gcaccacgag cgcccgcgga agccgggtcc gcaggaagtc ccggcactgc
     2521 cccagcgcct gcgggtgcga gtggacctcg cggacggatt cgaccgtagt cccggcgacc
     2581 gccatcaagt gatggcggac gcggacctgc acctcgccca cgatccgcac gtcgtgttcg
     2641 cgcagcaggt cgtagttcgt atgcacgctg ccgtggagcg agttctcgat cggcgccact
     2701 gccaggtccg ccgttccgtc gacgagcgcc tcgaagacgg cctcgaagga ggggaggggc
     2761 cggaggtcgg cccccggaac gaaggcgagc gccgcttcct cgctgtaggc gccgatctct
     2821 ccctggaacg tgacggtcat ggggctacat ccggtcgggg gcggagatgc ccagcacggc
     2881 cagtccgttg cggagcacga tgcggacggc gagggcgagt gccatgcgcg cgctcgcgat
     2941 gtcgggcggc tggccgatga tccggctcgc gccgtagaac tgcgtgaacg ccacggccac
     3001 gtcgcgcagg tagaccgcca cgcggtgcgg ctcgcgggcc tcggccgccc ggacgatggc
     3061 ttcggggaag ccgagcagca ccttgacgag ggccagctcc gtctcgtggt cgagcagcga
     3121 caggtcggcc tcg
//
LOCUS       DBNHQZ010000351         4660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2407476, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000351 DBNHQZ010000000
VERSION     DBNHQZ010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2407476"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="AC16YU_09505"
     CDS             complement(<1..2259)
                     /locus_tag="AC16YU_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6065503.1"
                     /translation="MRLRGAIGPVLLALAWVLAASPAAGQRWGSVEGRVTDGASGDPI
                     AGVSVVVTGTNFGTASDDTGRYVLRMPDGRYGVTFSAVGYAAHRDSAVVRFGAVARLD
                     VALATSTTELDEVTIEETRQVAEAGVFEISPRDALDIPAPVRDIARMLKMLPGVATNN
                     ELSNQVSVRGGGFNENLFFLNGFEIFLPFRPRQGEQEGLSLLNPDLAERIVFYSGGFP
                     ARHGGKLSSAVEVEYRRPERGSGAPTGTIHASLLEAGLAASGRAGGGRVGWLVGARTA
                     RARRFFATQELKGDYDPEYTDVQASLGWDLGAGTRVEAVGVWARHAFRLSPDARRTFF
                     GTYSQDPDLAPSNLQSLWIRYDADNSERDGYETGFGGLRLVNRFTGGRRMEHDVAVFS
                     TAETERFDLSGTAVLYLVDPSDPDPEPGSGLFPAGTSRQEDAADNRVDVRSVTAQGRY
                     AAPLGGHLVEIGWQARREHFSDRLDEKSTVSGQSTDGTPVRIVVDSLRDSAELDAWRT
                     AVFAQDMVDLFPGRPGTATLTAGMRADWFDFNDEWTLSPRVSVRYRVSDQVQLVGAWG
                     IYYQSPSYRELRGRPQPGETILGALNRSLRSQRSNQVVAGVEYFVPRLRLSLRAEAYA
                     KWLDRLVSYDIDNVRVRYSGRNDARGYARGLDLQLRGEFVPGMESFLNYSFLVAREEF
                     LPAYVDEYTTGLSPRPTDQRHTVSLLVRDYIPTDPDWRLHLRMLFGSGLPYTPPVPGR
                     ELGSGLVVQEPGE"
     gene            complement(2429..3901)
                     /gene="proS"
                     /locus_tag="AC16YU_09510"
     CDS             complement(2429..3901)
                     /gene="proS"
                     /locus_tag="AC16YU_09510"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR6065504.1"
                     /translation="MADAITPRSQDYSQWYHDVVRHARLADYSPVRGCMIVRPNGYAL
                     WENMQAALDAMFKATGHRNAYFPIFIPQSFLAREAAHVEGFAKECAVVTHSRLRVGAD
                     GNLDVDPDSKLEENLIVRPTSETIVWDTYAKWIQSWRDLPILLNQWNNVVRWEMRTRL
                     FLRTMEFLWQEGHTAHATREEAMEETLRILDIYATFAEEWMAVPVIKGVKSASERFAG
                     ADETFCIEALMQDGKALQAGTSHFLGQNFARAFNCQFQNRDNELEYVWATSWGVSTRL
                     IGALVMVHSDDRGLVLPPRLAPTQAVIVPIWRSDEDRAAVLEASAGIEARLRGAGVRV
                     ELDAREGYRPGWKFTEHEVQGVPVRLAVGPRDVANGTVELARRDGAEKQSVPADTIAR
                     SVEEALASMQRGLYDRALALREANTTSVDSYDDLRAVLDGPGGFARMHWDGTPETEAR
                     IKDETKATIRCIPLEGYEEDGVDPVSGRPSRGRVLYARAY"
     gene            4177..>4660
                     /locus_tag="AC16YU_09515"
     CDS             4177..>4660
                     /locus_tag="AC16YU_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065505.1"
                     /translation="MTHETLRPNPLRWFTGVVAPLLVLIGLSGCYTKLAVVDHEWDEG
                     PDYVETEVVDENGEVVVTREYYDDGYYDGGYYDDNWYEPYRYRRYFSRYYDPFYDPFW
                     SSSWYDPYWYGGAWGPYHSGFSLSIGWGWNSWYGYNDWGWYNPYRYSYGGWYSPYRYG
                     G"
BASE COUNT          720 a         1594 c         1630 g          716 t
ORIGIN      
        1 ctcgcccggt tcctgcacga cgagcccgct gcccagctcg cgcccgggga cgggcggcgt
       61 gtacggcagg cccgagccga agagcatgcg caggtgcagg cgccagtccg ggtcggtcgg
      121 gatgtagtcg cgcaccagga gcgagaccgt gtgcctctgg tcggtggggc gggggctcaa
      181 gccggtcgtg tactcgtcga cgtacgcggg caggaattcc tcccgggcca cgaggaagct
      241 gtagttgagg aagctctcca tccccggcac gaactccccg cggagttgca ggtccaggcc
      301 gcgggcatac ccccgggcgt cgttgcgccc cgagtaccgc acacgcacgt tgtcgatgtc
      361 gtacgagacg aggcggtcca gccacttggc gtaggcctcc gcccggagcg acaggcggag
      421 gcgcggcacg aagtactcga cgcccgcgac cacctggttc gagcgctggg aacggagcga
      481 gcggttcagc gcacccagga tcgtttcgcc cggctgcggg cgtccgcgca gctcgcggta
      541 ggaggggctc tggtagtaga tgccccacgc gccgacgagc tgcacctggt cgctcacccg
      601 gtaccggacg gacacgcgcg gcgagagcgt ccattcgtcg ttgaaatcga accagtcggc
      661 gcgcatgccg gccgtgagcg tggccgtgcc cggccggccg gggaagaggt cgaccatgtc
      721 ctgcgcgaag acggcggtgc gccacgcgtc gagctcggcg ctgtcacgca ggctgtcgac
      781 cacgatccgc accggcgtgc cgtcggtcga ctggccggag acggtggact tctcgtcgag
      841 gcggtcggag aaatgctcgc ggcgggcctg ccagccgatc tcgaccaggt ggcctccgag
      901 cggggcggcg tagcgcccct gggcggtcac ggagcgcacg tcgacccggt tgtccgcggc
      961 gtcctcctgg cgcgacgtgc cggccgggaa caggcccgag cccggctccg ggtcgggatc
     1021 ggacggatcc accaggtaca ggacggccgt gcccgagaga tcgaagcgct cggtctccgc
     1081 cgtcgagaag accgccacgt cgtgctccat gcgccgtccg cccgtgaacc ggttcacgag
     1141 gcgcaggccg ccgaaacccg tctcgtagcc gtcgcgctcc gagttgtccg cgtcgtagcg
     1201 gatccagagc gactgcaggt tgctgggcgc gaggtccggg tcctggctgt acgtcccgaa
     1261 gaacgtgcgc cgcgcgtccg ggctcagccg gaacgcgtgc cgcgcccaca cgccgaccgc
     1321 ctccacgcgc gtgccggccc cgaggtccca tccgagactc gcctggacat cggtgtactc
     1381 cgggtcgtag tcacccttga gctcctgggt cgcgaagaag cgccgggccc gcgccgtgcg
     1441 cgcgccgacg agccagccga ccctcccgcc gcctgcccgg cccgaggccg cgagccccgc
     1501 ttcgagcagc gaggcgtgga tcgtgccggt cggcgccccc gacccgcgct ccggcctgcg
     1561 gtactccacc tcgaccgcgg acgagagctt gcccccgtgc cgcgccggga agcctcccga
     1621 gtagaacacg atgcgctcgg ccaggtccgg attgagcagg ctcagtcctt cctgctcgcc
     1681 ctggcgcggc cggaacggga ggaagatctc gaatccgttc aggaagaaca ggttctcgtt
     1741 gaatccgccg ccgcgcaccg acacctggtt cgacagttcg ttgttcgtcg cgacgcccgg
     1801 gagcatcttg agcatgcggg cgatgtcgcg gacgggagcc ggaatgtcga gcgcgtcccg
     1861 gggcgagatc tcgaagacgc cggcctcggc gacctggcgc gtttcctcga tggtcacctc
     1921 gtcgagctcg gtcgtcgagg tcgcgagagc gacgtcgagc cgcgccacgg cgccgaaccg
     1981 gaccacggcc gagtcccggt gcgccgcgta accgacggcc gaaaaggtca ccccgtaccg
     2041 cccgtccggc atgcggagca cgtagcgtcc cgtgtcgtcg gacgcggtcc cgaagttggt
     2101 ccccgtgacc acgacgctca cgccggcgat cggatcgccg ctcgcgccgt ccgtgacccg
     2161 gccctctacg gagccccacc gctgtcccgc ggccggggag gccgcaagaa cccatgcaag
     2221 ggcaagaagg accggaccga tggcgccgcg cagccgcatg ttcggacgga gagaacccgg
     2281 acggaaaaag agactcgtag cggtgggcgc gcggacggaa ctcccggaat cgtggtggtc
     2341 aggctccgaa cggcagcccg ggtccttcgt tccgggcggc cggaccggga agacggaggg
     2401 cgctggggcg cccggcgcaa cgcccgggtc agtaggcgcg ggcgtacagc acgcggccgc
     2461 gggacgggcg tccggagacg ggatccacgc cgtcctcctc gtagccctcg agcgggatgc
     2521 accggatcgt ggccttcgtc tcgtccttga tgcgggcctc ggtctcgggc gtgccgtccc
     2581 agtgcatgcg cgcgaagccg cccgggccgt ccaggacggc gcgcaggtcg tcgtaggaat
     2641 ccacggacgt cgtgttcgcc tcgcggagcg cgagggcccg gtcgtagagc ccgcgctgca
     2701 tggaggccag cgcctcctcc acggatctgg cgatcgtgtc ggccgggacg ctctgcttct
     2761 cggctccgtc gcgtcgcgcg agctcgaccg tgccgttcgc gacgtcgcgc gggccgacgg
     2821 cgagcctcac ggggacgccc tgcacctcgt gctcggtgaa cttccacccc gggcgatagc
     2881 cttcccgggc gtcgagctcc acccgcacgc cggcgccccg cagacgggcc tcgatgccgg
     2941 cggaggcctc gagcacggcc gcgcgatcct cgtccgagcg ccagatcggg acgatcaccg
     3001 cctgcgtggg cgcgagcctg ggcggcagca cgagcccgcg gtcgtccgaa tggaccatga
     3061 ccagcgcgcc gatgagccgc gtcgatacgc cccacgaggt ggcccagacg tattcgagct
     3121 cattgtcgcg gttctggaac tggcagttga acgccctggc gaaattctgc cccaggaaat
     3181 ggctcgtgcc cgcctggagc gccttgccgt cctgcatcag ggcctcgatg cagaaagtct
     3241 cgtcggcgcc ggcgaaccgc tcgctcgcgc tcttgacacc cttgatgacc ggcacggcca
     3301 tccattcctc ggcgaacgtc gcgtagatgt ccaggatgcg gagggtctcc tccatggcct
     3361 cctcgcgcgt ggcgtgggcc gtgtggcctt cctgccacag gaattccatg gtcctcagga
     3421 agaggcgcgt gcgcatctcc cagcgcacca cgttgttcca ctggttcagc aggatcggga
     3481 ggtcgcgcca ggactggatc cacttggcgt acgtgtccca gacgatcgtc tcgctggtcg
     3541 ggcggacgat caggttctcc tcgagcttcg aatccggatc gacatcgagg tttccgtcgg
     3601 cgccgacgcg caggcgggag tgcgtgacga ccgcgcactc cttcgcgaag ccctcgacgt
     3661 gcgccgcctc gcgggcgaga aaggactgcg ggatgaagat ggggaagtac gcgttccggt
     3721 ggccggtggc cttgaacatt gcgtcgagcg ccgcctgcat gttctcccac agggcatacc
     3781 cgttcggtcg gacgatcatg cacccgcgca cgggcgagta gtccgccagc cgcgcatggc
     3841 gaacgacgtc gtggtaccac tgcgaatagt cctgcgagcg gggggtgatt gcgtcagcca
     3901 tcgtgtacag tgggtggacg gtatcgtcta caggaagggg cgaaagggcg cctatatacg
     3961 ggcctccggg aggcgtcagc cgatttggac cggtctttgc cgtaatttca aggtagcctg
     4021 aacggcccgg aaaccccaat gcgcggcgaa tgcacacccc aacggaaccc gtccgcgggg
     4081 ggtgcccgat ttgccccggt ttccggtcga tttcggtaac attttcgcac ggtaccggta
     4141 gcaacataga cagacacccc tcaggaggac attcccatga cgcacgagac actccgcccg
     4201 aacccgcttc gatggttcac gggagtggta gccccgctcc tggtgctcat cgggctgtcc
     4261 gggtgttaca ccaagctggc ggtcgtcgac cacgagtggg acgagggtcc cgactacgtc
     4321 gagacggaag tcgtggacga gaacggtgaa gtggtcgtga cccgcgagta ttacgacgac
     4381 gggtactacg atggtgggta ctacgacgac aactggtacg agccgtaccg gtatcgccgg
     4441 tacttctccc ggtactacga tccgttctac gatcccttct ggtcctcgag ctggtacgat
     4501 ccgtactggt acggcggggc ctggggcccg taccacagcg gcttctcgct gagcatcggc
     4561 tggggctgga acagctggta cggctacaat gactggggct ggtacaaccc gtaccggtac
     4621 tcctacggtg gctggtattc gccctaccgc tacgggggct
//
LOCUS       DBNHQZ010000352         7025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3490767, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000352 DBNHQZ010000000
VERSION     DBNHQZ010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3490767"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1208
                     /locus_tag="AC16YU_09520"
     CDS             189..1208
                     /locus_tag="AC16YU_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HIR6065506.1"
                     /translation="MRRISLLLPLVLALACAQPETTPVPESAPTDAAEGVRMIPVDTP
                     SGTFHVWTRRVGDNPRIKVLLLHGGPGSTSEYFEVFDRHFPAAGVEYYYYDQLGSHRS
                     DQPENDSLWVTGRFVEEVEQVRQALGLGPDNFFLLGHSWGGILATEYALKYQENLKGL
                     IISNMMSSCPAYDRYAEEVLGPALPPDALAEILALEAAGDFANPRYQELLMTHYYPKH
                     VLRRPVEEWPEAAVRGFEHMNPDIYVLMQGPSEFGISGRLETWDRSADLGRIAVPTLV
                     IGAAHDTMDPSHMEWMAGQVQNGRYLHCPNGGHMAMWDDEETYVTGVLDFLGDVDSGT
                     FGSDS"
     gene            1221..1754
                     /locus_tag="AC16YU_09525"
     CDS             1221..1754
                     /locus_tag="AC16YU_09525"
                     /inference="COORDINATES: protein motif:HMM:NF022152.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HIR6065507.1"
                     /translation="MDFETGLWPNVLGLTGVAFNIGWPFFRGRSAMLWAQAAGCFAFA
                     LHFMLLDADTGSLMSMLAGAQAVLAIPLGRDPRFRVSYLAMLPVIAVAMALTWMGTPS
                     AFAAVAMAVTSIGRYQVSEVRFRALMLACVPFWSGHNILVGSIPGLLSDALSFSAGAW
                     MLAVTLRRLRPAASPMA"
     gene            1839..2339
                     /locus_tag="AC16YU_09530"
     CDS             1839..2339
                     /locus_tag="AC16YU_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010140023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6065508.1"
                     /translation="MSHPIDPRVSVGHVHLKVSDLERSIAFYRDVLGFRLVAQIGPQA
                     AFLSAGDYHHHIGLNTWESLGGPAPPRGTTGLYHVAFLYPDRATLADAVRRVTGAGIR
                     FDGASDHGVSEALYLRDPDGNGVELYRDRPRKEWPRDPDGNLVLVNRPFDVAALLAES
                     PPAAKA"
     gene            2368..3522
                     /locus_tag="AC16YU_09535"
     CDS             2368..3522
                     /locus_tag="AC16YU_09535"
                     /inference="COORDINATES: protein motif:HMM:NF017495.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR6065509.1"
                     /translation="MIEHPLAVAAVLLGAILFAVSMTRRFTWAARLSTVLWVIFTGAL
                     VSNVGLVPKDAPLYGSLVDFTVPFAVCVILFTVNLSDVRKAGKAVLAAFGLATIGTVF
                     GVMVSGLVLEPYLADILGEEAWKLAGPYTGTYVGGSLNFFALWEGLELGRPDLFAAAN
                     AVDNLTLFPLYAAWMVIPTWLVGRYATRRQWQVDRSLHARGPDDRAPSLVPAHVVALA
                     FLALLVMALSEWIKTAWIDPVAPAIPTILVVTTLALVVGQFPAVRRLEGAWEIGDLAF
                     YVFFVSVGAMIDFYQAVVLSPILFAYVLVIMAGHFVFVFGVGRLLGMDPAVLTIASVA
                     TKAGPPLVPAVAHAKGWEHLVLPGIVAGMLGYAIGNYVGFAAAYLMRALL"
     gene            3539..4828
                     /locus_tag="AC16YU_09540"
     CDS             3539..4828
                     /locus_tag="AC16YU_09540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065510.1"
                     /translation="MRPLVLAVLLALFIAPDSRAHQAPGPIPLPPDLEVELAPSALPP
                     HLQDGATVPPSDVPWPTERWTRSDPEAEGMDPGPLARLDSLVRSGSTGLVDRLVVVRN
                     ARLVVDERYARDYFEASRGRESAIGCGWQGCAGDPSRIEDPYNYLHPSSHPYWHGSDL
                     HSVQSVTKSVAATVLGAALHRGDLPGLDVPLLTYFRDRDLSRVDPRLSTATLEDLLTM
                     RTGIEWHETDRPLDETNTTLRLERSEDWIRFTLEQPMDAEPGVKWAYSSGGSHLMSGV
                     IRSATGRFMDDYAREVLFGPLGIRSFHWKKTPSGYPDTEGGLYISATDLAKIGLLYLH
                     DGTWDGTRILPEGWARTATAVHVPNTLSAAGYGYQWWRLDRDGVEIWAGQGFGENYLL
                     VLPEYDVVAVVQSWNLFGAPSTSIRNEAIAALLGSVR"
     gene            4962..5738
                     /locus_tag="AC16YU_09545"
     CDS             4962..5738
                     /locus_tag="AC16YU_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6065511.1"
                     /translation="MKVRSAIRPLALPLIPVVIAVLVLTAGRPAQDRGKEIFDTVCSA
                     CHSLTPPPLKAPPMLMVAGHYMQGTATQDAAREAMIRWIPAPDTARMILPAHAVTRFG
                     HMAPLPLPEDDLKAVVDYIFREWAAAEGPEPRVPMCEGTQCGQGMQHRHGMQPDSSGP
                     GMQCAQCGQGMPHRHGMQPDSMRPGMPMRHGMQRDSSRQGMPMRQGMQPGLGMQMRHG
                     MAPDTSSLGGAMMCRRVEGQAGCAGGACAGGMCRKMQQGQ"
     gene            complement(5732..>7025)
                     /locus_tag="AC16YU_09550"
     CDS             complement(5732..>7025)
                     /locus_tag="AC16YU_09550"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR6065512.1"
                     /translation="PVASLFYTYYERSDVADRSGRPLMISFNGGPGSASLWMHLGYTS
                     PKQLLIDDEGYPVQPYGVRDNPHSIIDVADIVYVNPVNTGFSRIVGDAEREQFFGVNE
                     DVAYLAGWIDTFVSRRGRWTSPKFLIGESYGTTRVSGLAARLQDAHWMFLNGVILVSP
                     TGLGIDRDGPVRDAIALPYFTATAWYHGRLGPDLQARDLEELLAESERFTIEEYLPAL
                     AHGGRIDDERRRWIADQVSRLAGVSAGFVRDHNLRIPTSHFWKELLREEGYTVGRLDS
                     RYRGLDRQDFGETPDYNQELTSWNHAFAPAINHYLRDVLGVETDLRYWVFGPVSPWNR
                     EGDETGENLRSAMAKNPKLHVMVQSGYFDGATDYFSAKYTLWHMDPSGRLADRMRFEG
                     YRSGHMMYLRAEDLATSNEHIREFIRDALQTASVPARY"
BASE COUNT         1025 a         2539 c         2344 g         1117 t
ORIGIN      
        1 aacgcgaccg tggcggccat gctggcctac ctggcgtccg aagacccgga gacgaccggg
       61 cgcgagaaag ccctcctccc cgtggacccg cggacgggcg agccgcgcgg gtggcgcgac
      121 tgccgcccac cccgccgctc ggacacgcgc tgaacgcctg ccgacccacc atcccgaccg
      181 tccagaccat gcgccgcatc agcctgctgc ttcccctcgt cctcgccctc gcctgcgcgc
      241 agcccgagac cacgcccgtc cccgaatcgg ccccgaccga cgcggccgag ggcgtccgca
      301 tgatccccgt cgacacgccc tccggcacgt tccacgtgtg gacccgccgg gtcggcgaca
      361 atccacggat caaggtcctc ctgctgcacg gcgggccggg gtcgacgagc gagtacttcg
      421 aggtcttcga ccggcatttc ccggcggccg gcgtcgagta ctactactac gaccagctgg
      481 gctcccaccg cagcgaccag cccgagaacg attccctgtg ggtcaccggg cggttcgtcg
      541 aggaggtcga gcaggtgcgg caggcgctgg gcctcggccc ggacaacttc ttcctgctgg
      601 ggcactcgtg gggcggcatc ctggcgacgg agtatgccct caagtaccag gagaacctga
      661 agggactcat catctcgaac atgatgtcca gctgcccggc ctacgaccgg tacgccgagg
      721 aggtgctcgg accggccctg ccgccggacg ccctcgcgga gatcctcgcg ctcgaggccg
      781 cgggcgattt cgccaacccg cgctaccagg agctcctgat gacgcactac tacccgaagc
      841 acgtcctgcg caggccggtc gaggagtggc ccgaggccgc cgttcgcggg ttcgagcaca
      901 tgaaccccga catctacgtc ctgatgcagg gaccgagcga attcggcatc agcggccggc
      961 tcgagacctg ggaccggtcg gcggacctgg gacggatcgc cgtgcccacg ctggtcatcg
     1021 gagccgcgca cgacacgatg gacccgtcgc acatggagtg gatggccggc caggtgcaga
     1081 acggccgcta cctccactgc ccgaacggcg gccacatggc gatgtgggac gacgaggaga
     1141 cctacgtgac cggcgtcctg gatttcctcg gggacgtgga ttcgggcacg ttcgggtcgg
     1201 attcctgacc cgtaaagcgc atggacttcg aaacggggct ctggcccaac gtcctggggc
     1261 tcacgggcgt cgccttcaac atcggctggc cgttcttccg gggccgctcg gccatgctct
     1321 gggcgcaggc cgccgggtgc ttcgccttcg ccctgcactt catgctgctc gacgccgaca
     1381 cggggtccct catgtcgatg ctggccgggg cgcaggccgt gctcgcgatc cctctcggcc
     1441 gcgatccccg gttccgcgtg agctacctgg ccatgctgcc cgtgatcgcc gtcgcgatgg
     1501 cgctgacctg gatgggaacg ccgtcggcct tcgcggccgt ggccatggcc gtcacgagca
     1561 tcggccgcta ccaggtgagc gaggtccggt tccgcgcgct gatgctcgcc tgcgtaccgt
     1621 tctggagcgg ccacaacatc ctggtcggct caatccccgg gctgctttcg gacgccctct
     1681 ccttctcggc cggagcctgg atgctcgcgg tgacgctgcg ccggctgcgg ccggccgcct
     1741 cgcccatggc ctgagggcgc agcgccccgc ttcgtgaaac ttccgccaag gacgcgggat
     1801 aggatgtgat ctccagtgcc acggcccctg ccccagccat gagccacccc atcgatcccc
     1861 gagtgtccgt cggacacgtg cacctgaagg tatccgatct cgagcgctcc atcgccttct
     1921 accgggacgt actcggcttc cgtctcgtag cccagatcgg tccccaggcc gcgttcctgt
     1981 cggccggcga ctaccaccac cacatcggcc tgaacacctg ggagagcctc ggcggcccgg
     2041 caccgccgcg cgggaccacc ggcctgtacc acgtcgcctt cctgtacccc gaccgggcga
     2101 cgctcgccga cgcggtccgg cgcgtcaccg gggcgggcat ccggttcgac ggggcgtcgg
     2161 accacggtgt cagcgaggcg ctctatctcc gggacccgga cggcaacggc gtcgaactgt
     2221 accgcgaccg ccccaggaag gaatggccgc gggacccgga cggcaacctg gtcctggtca
     2281 accggccctt cgacgtggcc gcgctcctgg ctgaatctcc cccggcggcg aaggcctgac
     2341 ccccgtcacg cagcagagag gatcccgttg atcgagcacc ccctggccgt cgccgccgtc
     2401 ctcctcggcg ccatcctctt cgccgtgtcg atgacgcgac ggttcacctg ggcggctcgg
     2461 ctctccaccg tgctctgggt cattttcacg ggcgccctcg tctcgaacgt cgggctcgtg
     2521 cccaaggacg cgccgctgta cggatcgctc gtcgacttca ccgtgccgtt cgcggtatgc
     2581 gtgatcctgt tcaccgtgaa cctctccgac gtccgcaagg ccggcaaggc cgtgctcgcg
     2641 gcgttcgggc tcgccacgat cggcaccgtg ttcggggtga tggtctccgg gctcgtcctt
     2701 gaaccgtacc tggccgacat cctgggcgag gaggcatgga agctcgccgg gccttacacg
     2761 ggcacgtacg tcggggggag cctgaacttc ttcgccctgt gggaggggct cgagctcggg
     2821 cgaccggacc tcttcgccgc cgccaacgcc gtggacaacc tgacgctctt tcccctgtac
     2881 gcggcatgga tggtcattcc gacctggctc gtcggccggt acgcgacgcg gcggcagtgg
     2941 caggtggacc gaagcctcca cgccagggga cccgatgacc gcgcgccgag cctggtcccc
     3001 gcgcacgtcg tcgcactggc gttcctggcc ctcctggtca tggccctgtc ggagtggatc
     3061 aagacagcct ggatcgatcc ggtggccccg gcgatcccca cgatcctggt cgtcactacc
     3121 ctggcgctgg tcgtcgggca gtttccggcc gtgcggcggc tggaaggagc gtgggagatc
     3181 ggcgacctgg cgttctacgt gttcttcgtg tcggtcggcg cgatgatcga cttctaccag
     3241 gccgtggtcc tctcgcccat cctgttcgcg tacgtgctcg tcatcatggc gggccatttc
     3301 gtgttcgtgt tcggcgtcgg gcggctgctc ggcatggacc cggccgttct cacgatcgcc
     3361 tcggtcgcca ccaaggccgg cccgccgctc gtccccgccg tcgcccacgc gaagggctgg
     3421 gagcacctgg tgctgccggg catcgtggcc ggcatgctcg ggtacgcgat cggcaattac
     3481 gtcggattcg cggcggccta cctcatgcgc gcccttctct agacccggaa ccgattccat
     3541 gcgacctctc gtcctggccg tcctgctcgc gctcttcatc gccccggact cccgggccca
     3601 ccaggctcca gggccgatcc cgcttccgcc ggacctggag gtggagctcg ccccgagcgc
     3661 cctgcctccc cacctgcagg acggagcgac cgtgcccccg tccgacgtgc cctggcccac
     3721 cgagcgctgg acccggtccg atccggaggc ggaaggcatg gaccccgggc cgctcgcccg
     3781 gctcgactcg ctcgtccgct cgggctcgac cgggctcgtc gaccgcctgg tcgtggtccg
     3841 caatgcccgg ctggtcgtgg atgagcggta cgcgcgggac tacttcgagg cgtcccgggg
     3901 acgggagtcg gcgataggct gcgggtggca gggctgcgcg ggtgatcctt cgcggatcga
     3961 ggacccctac aattacctgc acccctcgtc ccatccgtac tggcacggat cggacctgca
     4021 cagtgtccag tcggtgacga agtcggtggc cgcgaccgtg ctcggagccg cgctgcaccg
     4081 gggagacctg cccggcctgg atgtcccgct cctgacgtac ttccgagacc gggacctgtc
     4141 gcgggtcgat ccccggttgt cgacggccac cctggaggac ctcctgacga tgcgcacggg
     4201 gatcgagtgg cacgagaccg atcgcccgct cgacgagacc aacacgacgc ttcggctgga
     4261 gcggagcgag gactggatcc ggttcacgct ggagcagccc atggacgccg agccgggggt
     4321 gaagtgggca tacagctccg gcggcagcca cctgatgtcg ggcgtgatcc ggtccgcgac
     4381 cggccggttc atggacgatt acgcgcgcga ggtcctgttc gggcccctcg gcatccggtc
     4441 gttccactgg aagaaaacgc cctcgggata cccggacacg gaggggggac tctacatctc
     4501 ggccacggac ctggccaaga tcgggctgct ctacctgcac gacggcacct gggacggaac
     4561 ccggatcctg cccgagggat gggcccgcac cgccacggcc gtgcacgtgc ccaacacgct
     4621 gtccgccgcc gggtacggat accagtggtg gcgcctggac cgggacggcg tcgagatctg
     4681 ggcagggcag ggtttcggcg agaactacct gctcgtcctg cccgaatacg acgtcgtggc
     4741 ggtcgtgcag agctggaacc tcttcggcgc cccgtcgact tccatccgga acgaggcgat
     4801 cgctgcgctt ctcgggtccg tccgctgatc gagacgacag gggactcccc tacacgagcc
     4861 gtctctatcg cctgccgcgg cgccttgcgc gggcggcgta ccgtttcgcg gttggattct
     4921 ccgaatgacc ccgcggaccc cttggtccgc ccgatgccgc catgaaagtc cgctccgcga
     4981 tccgtcccct cgccctcccg ctgattcccg tcgtcatcgc cgtgctggtc ctgacggccg
     5041 gccggccggc gcaggacagg gggaaggaga tcttcgacac ggtctgctcc gcgtgccact
     5101 cactcactcc tccgccgctc aaggcgcccc cgatgctgat ggtggccggg cactacatgc
     5161 aggggaccgc gacacaggac gccgcccgcg aggccatgat ccggtggatc ccggcgcccg
     5221 acaccgcgcg catgatcctg ccggcccacg cggtcacccg cttcgggcac atggcgcctc
     5281 tccccctgcc ggaggacgac ctgaaggcgg tcgtggacta catcttccgt gaatgggccg
     5341 ccgcggaggg accggagccg cgcgtgccca tgtgcgaagg cacccagtgt ggccagggca
     5401 tgcagcaccg gcacggcatg cagcccgact cgagcggccc gggcatgcaa tgcgcccagt
     5461 gcggccaggg catgccgcac cggcacggca tgcagcccga ctcgatgcgg ccaggcatgc
     5521 ccatgcgcca cggcatgcag cgcgattcga gccgtcaggg catgccgatg cgccagggca
     5581 tgcagcccgg cctcggcatg cagatgcgcc acggcatggc gcccgacacg agcagcctgg
     5641 gcggcgccat gatgtgccga cgggtcgaag ggcaggccgg gtgcgccggg ggcgcgtgcg
     5701 ccggcggcat gtgccggaag atgcagcagg gtcagtagcg ggcgggcacg gaagccgtct
     5761 gcagcgcgtc ccggatgaac tcccggatgt gctcgttcga cgtcgcgaga tcctcggcgc
     5821 gcaggtacat catgtggccg ctgcggtagc cctcgaaccg catccggtcc gcgagcctgc
     5881 ccgacggatc catgtgccag agggtgtact tcgccgagaa gtagtcggtc gccccgtcga
     5941 agtagcccga ctgcaccatc acgtgcagct tcgggttctt cgccatcgcc gaccggaggt
     6001 tctcgccggt ctcgtcgccc tcccggttcc aggggctgac cggcccgaat acccagtagc
     6061 gcaggtccgt ctccacgccc agcacgtcgc gcaggtagtg attgatcgcg ggcgcgaaag
     6121 cgtgattcca ggacgtcagc tcctggttgt agtcgggcgt ctccccgaaa tcctgccgat
     6181 ccaggccgcg gtagcgtgaa tcgaggcggc ccaccgtgta accctcctcg cgaagcagtt
     6241 ccttccagaa gtgagaggtc ggaatccgga gattgtgatc ccggacgaag ccggcggaca
     6301 cccctgccag gcgcgacacc tggtcggcga tccacctccg gcgctcgtcg tcgatccggc
     6361 ccccgtgcgc gagcgccggc aggtactcct cgatcgtgaa ccgctccgac tcggcgagca
     6421 gctcctcgag gtcacgcgcc tgcaggtcgg gcccgagcct gccgtgatac caggcggtcg
     6481 ccgtgaagta gggcagcgcg atggcatcac gcactgggcc gtcgcgatcg atgccaaggc
     6541 ccgtggggga gacgagtatg accccgttca ggaacatcca gtgcgcgtcc tgcagcctcg
     6601 cggccaggcc ggagacgcgc gtcgtgccgt agctctcccc gatcaggaac ttcggcgacg
     6661 tccagcgccc ccggcgcgag acgaacgtgt cgatccaccc cgcgaggtac gcgacgtcct
     6721 cgttcacccc gaagaactgc tcccgctccg catcgcccac gatgcgcgag aagcccgtgt
     6781 tcaccggatt cacgtagacg atgtccgcca cgtcgatgat cgagtgcggg ttgtcgcgca
     6841 cgccgtacgg ctgcaccggg tagccctcgt cgtcgatgag gagctgcttc gggctggtat
     6901 atccgagatg catccacagc gacgcgctgc ccggcccccc gttgaacgag atcatgagcg
     6961 ggcggccgga ccggtcggcc acgtccgagc gctcgtagta cgtgtagaag agggaagcga
     7021 cgggc
//
LOCUS       DBNHQZ010000353         4373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2407846, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000353 DBNHQZ010000000
VERSION     DBNHQZ010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2407846"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(599..670)
                     /locus_tag="AC16YU_09555"
     tRNA            complement(599..670)
                     /locus_tag="AC16YU_09555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(636..638),aa:Asn,seq:gtt)
     gene            complement(776..1048)
                     /locus_tag="AC16YU_09560"
     CDS             complement(776..1048)
                     /locus_tag="AC16YU_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065513.1"
                     /translation="MPCLFAAIALITPRLLMAYLWFFTNWFYGVFDTVMWPILGFLFA
                     PTSLLWYSVVHNAYDGQWGTLQIVVMVIAVLFDLSPGSGRRKGKRD"
     gene            1182..2327
                     /locus_tag="AC16YU_09565"
     CDS             1182..2327
                     /locus_tag="AC16YU_09565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR6065514.1"
                     /translation="MSVVSVSDAHRITVPQLDMPPRLLLGPGPSNAHPRVLQALSTPQ
                     VGHLDPTFIALMSEVQELLRYAWQTDNALTIPVSGTGSAAMEATLANSVEPGDVVVVG
                     VNGYFGERLCDMASRYGADVRRLEKPWGEVFDLDEIGDALDTHRPAILALVHAETSTG
                     ARQPIEGVGELCREMDTLLLVDTVTSLGGVPLFVDAWGVDMAYSGTQKCLSCPPGLGP
                     LTFGPRAMEKLERRRTRVPNWYLDLTAVGKYWGAERTYHHTAPINMNYGLREALRLVA
                     EEGLEARWERHRRNAQLLWDGLEALRLRCHVSAENRLPSLTTVRVPDGVDAKRVVQTL
                     LREYNIEIAGGLGQLAGKVWRVGLMGYNSRPENVARLLDALDRSLLH"
     gene            complement(2373..2978)
                     /locus_tag="AC16YU_09570"
     CDS             complement(2373..2978)
                     /locus_tag="AC16YU_09570"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR6065515.1"
                     /translation="MSSLSDLFERNRLWAARMRRTDPDMFDRLSEAQNPRYLWIGCAD
                     SRVPANQVIDLPPGTVFVHRNIANVVHVSDLSVLSVLQFAVEKLHVRDIIVCGHYGCG
                     GVAAAIRGTARGPLQHWLRGLRDLYREHREELDALDDLKRANRLSELNVLQQVRSIAE
                     NPIVEVAWNDGRPLTVHGLIYDLDDGHLHDLGLTVSRPVHA"
     gene            complement(3199..3759)
                     /locus_tag="AC16YU_09575"
     CDS             complement(3199..3759)
                     /locus_tag="AC16YU_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065516.1"
                     /translation="MADLIGAVWLPTLVAAVLVFLASWLMHTVLPHHKSDYAKVPDQE
                     GLLATLRAAGVKPGQYHFPYMDSPAAMKDPAFNETRTRGPVGFLTIGRSGPVTMGREL
                     SLHFVYCVVVSFLAGYIAFAALGVGADYLSVFRITGTAAWMAYSVAHASASIWFFQPW
                     SITWKYVFDGLVYALLTAGAFGWLMA"
     gene            3894..>4373
                     /locus_tag="AC16YU_09580"
     CDS             3894..>4373
                     /locus_tag="AC16YU_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR6065517.1"
                     /translation="MDAFRSLDPVLQALVAGVFTWFVTALGAGLVVFMRKMSQLLLDI
                     MLGFAAGVMTAAAVWSLLLPSIEVAGQQGLVPWVPAAIGFVAGGVFLRGADALLPHLH
                     PGLPRTQAEGVKTSWQRSTLLVLAITLHNIPEGLAVGVAFGAAGLGVEGASLAGALAL
                     "
BASE COUNT          730 a         1428 c         1550 g          665 t
ORIGIN      
        1 aaggacggcg gatcggagca tgggggacga acggcgcggc gttcggatcg tgcacgggga
       61 agataggaga ggggtgccgc gttcgcgtga tttcgcacgg tggggactcc ttgcgcgtcg
      121 gcggagaaaa cggatggggc gtaacgacga cctcgtgcat cagtggccgc caaccatcgg
      181 gagaatgaag tcgtgtcttc atcgtcagca gcgcgggatt cgtcggatcg gtaggccgga
      241 tccctgcata tcggtagcgt agccccctgc accgcagaag cgtaggccgt cggacgaagg
      301 ggcatcctgt cacgatccct tcgcgccggg caggcatcag gtgacgggtg gagcagggtc
      361 gccccgtcat ccgggatcag tcgagccgac gttcgatcca tacctcggcc acgacgacat
      421 ccgctggtcc aaggcggggc acgaggcatc cgcgaccaga atcacgaagg ccacttggag
      481 cactacggga cggatctgtg ctagcctcaa tggtccggac agtgcgactg ctccgctcga
      541 ccctgcctca cacaacggga aaaggccaga ccccggacgg gatcgggcct tcttcatgct
      601 ccccgggtag gattcgaacc tacaaccctg cggttaacag ccgcatgctc taccgttgag
      661 ctaccgagga atcgagcacc gtaaaatacg ggaccggatc gtgtcgatcc aaacgtaccc
      721 gattcgaagc gcccgtcaag aactggatcg tcgcttccgc aatcccggct ccccctcagt
      781 cccttttccc cttcctccgt ccgcttcccg gcgagaggtc gaacagcacc gcgatcacca
      841 tgacgacgat ctgcagcgtg ccccactggc cgtcgtaggc gttgtgcaca acggaatacc
      901 agagcagcga cgtcggggcg aacaggaagc ccaggatcgg ccacatgacc gtatcgaaga
      961 ccccgtagaa ccagttggtg aagaaccaca ggtacgccat gagcaggcgc ggcgtaatga
     1021 gcgcgatggc ggcgaatagg cagggcatgt tcgctcgggt ccggttcgga tgaaggaatg
     1081 tacgcgcccc ggagggaggg gttacggccg gccgccggtg gctatcttgg cgcccggcag
     1141 cgccacaaga agtccgatcg cggcagcgcc cgcgccaaac gatgtccgtc gtctccgtct
     1201 ccgacgccca ccggatcacc gtcccgcagc tcgacatgcc cccgcggctg ctgctgggcc
     1261 ctggaccctc gaatgcccat cccagagtgc tccaggccct gtccacgccc caggtgggcc
     1321 acctggaccc gacgttcatc gcgctcatgt ccgaggtgca ggagctcctc cgctacgcct
     1381 ggcagaccga caatgcgctc acgatcccgg tttccggcac gggcagcgcg gcgatggagg
     1441 ccacgctcgc gaactcggtc gagccgggtg acgtggtggt cgtcggggtg aacggctact
     1501 tcggcgagcg gctctgtgac atggcgtcgc gttacggcgc ggacgtgcgg cggctcgaga
     1561 agccgtgggg cgaagtattc gacctggacg agatcgggga cgcgctcgac acgcaccggc
     1621 cggccatcct cgcgctcgtg catgcggaga cgtcgaccgg ggccaggcag ccgatcgagg
     1681 gggtcggcga gctctgccgc gagatggaca cgctgcttct cgtggacacg gtgacgagcc
     1741 tgggcggcgt gcccctgttc gtggacgcct ggggcgtgga catggcctac agcggcaccc
     1801 agaagtgcct ctcctgtccg cccggcctcg gcccgctgac cttcggcccg cgcgcgatgg
     1861 agaagctgga gcgtcggcgg acgcgcgtgc cgaactggta cctggacctg acggccgtcg
     1921 ggaagtactg gggggccgag cgcacgtatc accacacggc gcccatcaac atgaactacg
     1981 ggctgcgcga ggcgctgcgc ctggtggcgg aggagggcct cgaggcgcgg tgggaacggc
     2041 atcgccgcaa cgcgcagctc ctgtgggacg ggctggaggc actccggctc cggtgtcacg
     2101 tttcggccga gaaccggctt ccgagcctga ccacggtgcg cgtgccggac ggagtagatg
     2161 cgaagcgggt cgtccagacg ctgctccgcg agtacaacat cgagatcgcc ggcgggctcg
     2221 ggcagcttgc ggggaaggtg tggcgggtgg gcctcatggg ttacaacagc cggcccgaga
     2281 acgtggcgcg gctgctcgac gccctcgatc gcagtctgct gcactgagcg ccgcagccgg
     2341 cccgccggaa ccgccgcggc cctcgtggag cgttacgcgt gcacgggccg ggagacggtc
     2401 agcccgaggt cgtggaggtg gccgtcgtcc aggtcgtaga tgagtccgtg gacggtgagc
     2461 ggtctcccgt cgttccaggc cacctcgacg atcgggttct cggcgatcga gcgcacctgc
     2521 tgcaggacgt tgagctctga caggcggttg gcccgtttca ggtcgtccag ggcatcgagc
     2581 tcctcgcgat gttcccggta caggtccctg aggccgcgaa gccagtgctg cagcggcccc
     2641 cgggccgtac cccggatggc ggcggccacg cccccgcagc cgtagtggcc gcagacgatg
     2701 atgtcccgta cgtgcagctt ctcgacggcg aactgcagga cggagagcac gctcaggtcg
     2761 gagacatgca ccacgttggc aatgttccgg tgcacgaaga ccgtgcccgg cggaaggtcg
     2821 atgacctggt tggccggcac gcgggagtcg gcgcagccga tccacaggta gcgcgggttc
     2881 tgcgcttccg acagccggtc gaacatgtcc gggtccgtcc ggcgcatgcg ggcggcccag
     2941 aggcggtttc gctcgaagag atcggacagg gaagacatgc cggcgcctcg gatggttcgc
     3001 gcgggaacgg ggcgggtcgg ccgccgcccc gcacggaaag ctaacgcgtg ccacgcagcg
     3061 aaacccgtcc ggcggccccg gatgcacgcg acggcgccga tccgccaggg gccgccggac
     3121 ggggggcgtt cctcctcccc aggaaagggg gccggccccg cacgggaccg gcccccgaat
     3181 cgtgcactga gacgcaggtc aggccatgag ccacccgaag gcgcccgccg tgagcagcgc
     3241 gtagacaagg ccgtcgaaca cgtacttcca ggtgatggac cagggctgga agaaccagat
     3301 cgacgccgaa gcatgggcca ccgagtaggc catccaggcc gcggtgcccg tgatgcggaa
     3361 cactgacagg tagtcggcgc ccaccccgag ggccgcgaac gcgatgtagc cggccaggaa
     3421 cgagacgacc acgcagtaca cgaaatgcag ggagagctcc ctgcccatcg tgacggggcc
     3481 ggaccgtccg atggtcagga atccgaccgg tcccctcgtc ctcgtctcgt tgaacgccgg
     3541 gtccttcatg gccgccgggc tgtccatgta ggggaagtga tactgtccgg gcttgacgcc
     3601 ggccgcgcgc agggtggcga ggaggccctc ctggtcgggg accttcgcgt agtccgactt
     3661 gtggtgcggc aggaccgtgt gcatgagcca gctcgcgagg aagacgagca cggcggcaac
     3721 cagcgtcgga agccagacgg caccgatgag atcagccatg ggggaccctc cgtgatgctt
     3781 ggatgaagga cgtttgaaag gtacatgaag gctccggcac gcgccatatg tgtatggaag
     3841 ggtgtcggga atccccgtac cgtaggcgcc gtccgacctc cgtccgcccc gtgatcgacg
     3901 ccttccgctc gctcgacccc gtgctccagg cgctcgttgc cggcgtgttc acgtggttcg
     3961 tcacggccct cggggcggga ctcgtcgtct tcatgcggaa gatgtcccag ctgctgctgg
     4021 acatcatgct tggcttcgcc gcgggcgtca tgacggccgc cgccgtctgg tccctgcttc
     4081 tgccctcgat cgaggtggcc ggccagcagg gcctggttcc gtgggttccg gcggcgatag
     4141 ggttcgtggc ggggggcgtc ttcctccggg gcgcggatgc gctgttgccg cacctgcacc
     4201 ccgggctccc ccggacgcag gccgagggcg tgaagacgtc gtggcagcga agcacgctcc
     4261 tcgtgctcgc gatcaccctg cacaacatcc cggaaggtct cgccgtcggg gtggccttcg
     4321 gtgccgcggg cctgggcgtg gaaggggcga gcctggccgg agccctggcg ctg
//
LOCUS       DBNHQZ010000354         3133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3763178, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000354 DBNHQZ010000000
VERSION     DBNHQZ010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3763178"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="AC16YU_09585"
     CDS             <1..935
                     /locus_tag="AC16YU_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6065518.1"
                     /translation="KNDFFLPMVHLQLNPTDWLTLRGAYTETLTRPDYIQYAPITSIN
                     NYRTYVRAANSLLKPAHSKNWDAAVSVYEPKLGLMTVAGFEKSIDDLIMQVRYVLHPD
                     VDLLPGMNVPEEWIADNPQADTYINNPYPATYRGFEVDWQTNFWWAPSIFKGIVLNAN
                     YTRIFSETDYQAYYLVDSDSIKQIRPRVYYKTIRDDSLRTGRMPDQPKHIANLTLGYD
                     YRGFSARLSFLYQTNTSTWINPTNPLFDNYSGDYSRWDLSVSQKLQSGLEIFANFNNL
                     NNRPDQNFRGSATENPTYIEYYGFTMDIGARFRY"
     gene            1033..>3133
                     /locus_tag="AC16YU_09590"
     CDS             1033..>3133
                     /locus_tag="AC16YU_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR6065519.1"
                     /translation="MRIRYALIAAIFAVAFAWSPPDAAAQSELIVEWETAPGSQLPLK
                     NALRDAIANDAGRPADRVYVLRRGGFYWLEDPVSWAGFHLHLRGQTAAEARPEDNVCG
                     AAFNEDCGYAIIQRVARADQSLTGTMLSNSGSGSHLTAENIWFMGKADTGARTAYEQI
                     ALNASDSRFVFDHVVFDQNDWHLLGPNASNCDMFVTNSSFRNLWGPTQQWEGLGVRFE
                     VGADSVVFENNTFLNIGFTPFQSEGAPMRYFLANHNTFVNIGRSFQAGNMWMEAYITN
                     NVFVNPFFHGEGSADYDNPDRVDPFAGFFSIGALPAIYGTNFDREIVLANNSYWRDPA
                     FAVYYADTIRAQPIVNDTTMGWFDQFSNMVFQDNFIDVDPGLAVYPDNIDGMIGNITD
                     LRAGITPARGYDWGIDEDCDVCSVWPLPEDFSYSNVALQTAGTDGLPLGNLNYWPSAK
                     DTYDANRDAYVGAIEGMATGIEITPISLVEGEDGVLSGDASVFVAPGFTHFFMQGSGY
                     IEWEFELASDMTIGLDVHTNMRSETQRGQRIKIDGGNTLRNHTGFGEYYFCTAAQAGC
                     AVPLPNNEWTTVEIRQGILIGDTGPLLNLTAGTHTVRIEPSWGWQGFSGIDITDNAGT
                     VLASFVATDADYQGVQAICDDSDYCPQFFKAATLNPGGQVEFNFDAPWSGQYLLRIFF
                     QTPSGAAVGEMLIDDVTE"
BASE COUNT          597 a         1055 c          977 g          504 t
ORIGIN      
        1 ggaaaaacga cttcttcctt ccgatggtgc acctgcagct gaatcccacc gactggctca
       61 cgctgcgcgg cgcgtacacc gagaccctga cacggccgga ctacatccaa tatgccccga
      121 tcacgtccat caacaactac aggacgtacg tgcgcgcggc gaactctctc ctgaagccgg
      181 cgcactcgaa gaactgggac gccgcggtct cagtgtacga gccgaagctc gggctgatga
      241 cggtggcggg attcgagaag tcgatcgacg acctgatcat gcaggtccgc tacgtgctgc
      301 acccggacgt ggacctgctg cccggcatga acgtgcccga ggagtggatc gccgacaacc
      361 cgcaggccga cacctacatc aacaacccgt acccggccac gtaccgcggt ttcgaggtcg
      421 actggcagac caacttctgg tgggcgcctt ccatcttcaa gggaatcgtc ctgaacgcga
      481 actacacgcg gatcttctcc gagacggact accaggccta ctacctggtc gactcggaca
      541 gcatcaagca gattcgcccg cgtgtctact acaagacgat cagggacgac tccctgcgca
      601 cgggccgcat gccggaccag ccgaagcaca tcgccaacct gacgctcggc tacgactacc
      661 ggggcttctc cgcgcggctg tcgttcctct accagacgaa cacgtccacg tggatcaacc
      721 ccaccaaccc gctcttcgac aactactcgg gcgactattc gcgctgggac ctgtccgtga
      781 gccagaagct gcagagcggg ctcgagatct tcgccaactt caacaacctg aacaaccggc
      841 ccgaccagaa cttccgcgga tcggcgaccg agaaccccac ctacatcgag tactacgggt
      901 tcaccatgga catcggggcc cgcttccgct actgaaaccc ggcgcaggtc ggcgcggccg
      961 cccgggccgt gccggcgacc gccctacgag cccattccct cgctcagcaa cccttccaac
     1021 ggaggaacga caatgagaat ccgatacgcc ctgatcgcgg ccatcttcgc ggtggccttc
     1081 gcctggtcgc cgcccgacgc ggccgcccag agcgagctca tcgtggagtg ggagaccgcg
     1141 ccgggatccc agctgccgct caagaacgcg ctccgcgatg cgatcgccaa cgacgccggg
     1201 cggcccgccg accgggtcta cgtgctgcgg cgcggcggtt tctactggct ggaagatccg
     1261 gtctcgtggg cgggcttcca cctgcacctc aggggccaga cggccgccga ggcgcggcca
     1321 gaggacaacg tctgcggcgc cgccttcaac gaggactgcg ggtacgccat catccagcgg
     1381 gtggcccggg cggaccagtc gctgaccggc accatgctca gcaactcggg ctccggcagc
     1441 cacctgaccg cggagaacat ctggttcatg ggcaaggccg acacgggcgc gcggacggcc
     1501 tacgagcaga tcgcgctcaa cgcgtcggac tcgcggttcg tgttcgacca cgtggtcttc
     1561 gaccagaacg actggcacct gctcggcccg aatgcctcga actgcgacat gttcgtcacg
     1621 aactcgtcgt tccggaacct gtggggcccg acccagcagt gggagggcct gggcgtacgg
     1681 ttcgaggtgg gcgccgactc ggtcgtgttc gagaacaaca cgttcctgaa catcggcttc
     1741 acgcccttcc agtcggaggg cgcgccgatg cggtacttcc tcgcgaacca caacacgttc
     1801 gtgaacatcg gacgctcctt ccaggccggc aacatgtgga tggaggcgta catcaccaac
     1861 aacgtgttcg tgaacccctt cttccacggc gagggcagcg ccgactacga caatcccgac
     1921 cgggtggacc cgttcgcggg attcttctcg atcggggctc ttccggcgat ctacggcacc
     1981 aacttcgacc gggagatcgt gctggctaac aactcgtact ggcgtgaccc cgcgttcgcg
     2041 gtctactacg cagacacgat ccgggcccag ccgatcgtca atgacacgac gatgggctgg
     2101 ttcgaccagt tcagcaacat ggtcttccag gacaacttca tcgacgtgga tcccgggctc
     2161 gccgtttacc cggacaacat cgacgggatg atcggaaaca tcacggacct gcgcgccggg
     2221 atcaccccgg cccgggggta cgactggggc atcgacgagg actgcgacgt gtgcagcgtc
     2281 tggccgctgc ccgaggactt ctcgtactcg aacgtggcgc tgcagacggc cgggaccgac
     2341 gggctgccgc tcggcaacct gaactactgg ccctcggcca aggacacgta cgacgcgaac
     2401 cgcgacgcgt acgtcggcgc gatcgaaggg atggcgaccg gcatcgagat cacgccgatc
     2461 tcgctcgtgg agggcgagga cggtgtgctt tcgggcgatg cttcggtctt cgtggcgccg
     2521 ggcttcaccc acttcttcat gcagggaagc gggtacatcg agtgggagtt cgagctcgcg
     2581 agcgacatga cgatcgggct cgacgtccac acgaacatgc gctcggagac gcagcgcggc
     2641 cagcggatca agatcgacgg cggcaacacg ctgcggaatc ataccggatt cggcgagtac
     2701 tacttctgta cggcggcgca ggccgggtgc gccgtgccgc tgccgaacaa cgagtggacc
     2761 acggtcgaaa tccggcaggg catcctgatc ggggacaccg gaccgctgct gaatctcacc
     2821 gccgggaccc acacggtgcg gatcgagccg tcgtggggct ggcagggctt cagcggcatc
     2881 gacatcacgg acaacgcggg gaccgtcctg gcgtcgttcg tggccacgga tgccgactac
     2941 cagggcgtgc aggcgatctg tgacgattcg gattactgcc cgcagttctt caaggcggcc
     3001 acgctgaatc cgggcgggca ggtcgagttc aatttcgacg cgccgtggag cggacagtac
     3061 ctgctgcgca tctttttcca gacgccatcc ggcgccgcgg tcggagagat gctcatcgat
     3121 gacgtgaccg agg
//
LOCUS       DBNHQZ010000355         2585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2001863, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000355 DBNHQZ010000000
VERSION     DBNHQZ010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2001863"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1999
                     /locus_tag="AC16YU_09595"
     CDS             251..1999
                     /locus_tag="AC16YU_09595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6065520.1"
                     /translation="MHPLRAFWILPLLALPLTGSSDRYPRDYFRSPVDIPLSLSGNFA
                     EMRSNHFHTGLDIRTNGQAGYRIYAAADGWIARVAVSPGGYGNALYVNHPNGYQTVYA
                     HLDRFEGPVVDYVESAQYARRSFAVDLYPSEGRFPVKKGEVIAISGNSGSSGGPHLHF
                     EIRESATAHPVNPLLFGMPVVDTTRPRLYRVKLYPEGRTSRVRVLGSGGRVLDRASDG
                     ESVSVTVEAAGAGRYALRPGTRIEAVGRVGFGIQTTDFHDLSSGRLGVYRVRLTAGER
                     PVFESEMEKLDFALQRYLNAHVDYEEHSRNGRWIQRSHLLPGNRLRIYRTERSGYVEF
                     EPGDTLTMRYDVEDAHGNAVSLTFPVRGMEEAGPAEPDTSLVPVAWNRSTAIERPGIV
                     IRIPDGALYASTGLRYAVERRGPGTFSPVHRVHDEHIPIHAAVSVSIEADSLPEALRS
                     RALVARRTRSGSVVSHGGSWQDGSVTAQVRTLGDFFVAVDTTAPAIRPMNVQGGANLS
                     GSRSIRFRIRDELSGIESYEGLVDGEWVLFAYDAKSNLIEYTFDGRVARGSHVLEVRV
                     TDGVGNRSVWRGPFTR"
     gene            complement(1989..>2585)
                     /locus_tag="AC16YU_09600"
     CDS             complement(1989..>2585)
                     /locus_tag="AC16YU_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6065521.1"
                     /translation="AVLVVPSGNLGNLTAAVLARLSGAPVRALVAAHNANRGFPGYLE
                     GGPRPAAPSVRTLSNAMDVGAPSNFERLEALLGAEGMRRGIRGLSVSDDDTIGAMRRT
                     FEETGYVCDPHTAVGLEAVRRLAKEGLRGPFLVMATAHPAKFPETVERAVGRRVPEPD
                     SLAALGIPDMRAPRCEASADDLLELLESTSPAGPALSG"
BASE COUNT          408 a          884 c          905 g          388 t
ORIGIN      
        1 acggcggtcg gcaggtgccg ctgcctgagg tgctggacga tgggcttgaa gatgatggac
       61 aggaggagtg ccaccacgaa cggaatcagt accgatcgca gctccagcag gacgacgccg
      121 acgaagaaga cggcgatcac tcccagcacg acaccgatca gattatagtt cttcatcgga
      181 cggatgcgtc aaaacgttcc ggggcctcgg gcgtacgcgg cggggaagaa acccgcgaat
      241 tgccgatttg atgcaccccc tacgcgcctt ctggattctg ccccttctcg cgctgccgct
      301 caccgggtcg tccgaccgct atccccggga ctacttccgg tctcccgtgg acattccgct
      361 ctcgctctcg ggcaatttcg ccgagatgcg gtcgaaccat ttccatacag gactcgacat
      421 ccggacgaac ggtcaggccg ggtaccgcat atacgcggcg gcggacggct ggattgcgcg
      481 ggtcgcggtc tcgccaggcg ggtacggcaa tgcgctgtac gtgaaccacc cgaacgggta
      541 ccagacggtg tacgcccacc tggaccgctt cgaggggccg gtggtggact acgtggagtc
      601 ggcgcagtac gcgcgccgct cgttcgcggt ggacctgtac ccgagcgagg gacgattccc
      661 ggtgaagaag ggtgaggtga tcgcgatctc gggcaattca ggcagctcgg gaggcccgca
      721 tctccacttc gaaatccgcg agtcggcgac ggcccatccc gtcaaccccc tgctcttcgg
      781 catgcccgtc gtggacacga cgcggcccag gctgtaccgc gtcaagctgt atcccgaggg
      841 acggaccagc cgggtgcgcg tgctcggaag cggcgggcgg gtcctggacc gggcgtccga
      901 cggcgagtcg gtgtccgtga ccgtggaggc ggccggcgcg gggcggtacg ccctgcggcc
      961 ggggacgcgc atcgaggcgg tcggacgcgt cggtttcggc atccagacca cggactttca
     1021 cgacctgtcc tcgggccgcc tgggcgtgta ccgggtccgg ctcaccgcgg gagagcgtcc
     1081 ggtcttcgag tcggagatgg agaagctgga cttcgcgctg cagcggtacc tgaacgctca
     1141 cgtcgactac gaggagcact cgcgcaacgg ccgctggatc cagaggagcc acctgcttcc
     1201 gggcaaccgg ctccggatat accggaccga gcgcagcggg tacgtggagt tcgagcccgg
     1261 cgatacgctg accatgcggt acgacgtcga ggatgcgcac gggaacgcgg tatcgctgac
     1321 cttccccgtg cgcggcatgg aggaggccgg tcccgcggag cccgacacgt cactcgtgcc
     1381 cgtcgcctgg aaccgctcca cggcgatcga gcggcccggc atcgtgatcc gcattccgga
     1441 cggcgccctg tacgccagca cgggactccg gtacgcggtc gagaggcgcg gtccgggcac
     1501 gttctcgccc gtgcaccggg tgcatgacga acacattccc atacacgccg cggtctcggt
     1561 ctcgatcgag gcggactcgc tgccggaagc ccttcggagc cgcgccctcg tggcccgtcg
     1621 cacgcgcagc ggatccgtcg tctcgcacgg cgggtcctgg caggacggca gcgtcacggc
     1681 gcaggtgcgc acgctcgggg acttcttcgt ggcggtcgac acgacggcgc ccgcaatccg
     1741 cccaatgaac gtccagggcg gtgcgaacct gtcgggatcc cggtccatcc ggttccggat
     1801 ccgggacgag ctctcgggaa tagagtcgta cgagggcctt gtcgacggcg agtgggtgct
     1861 cttcgcctac gacgcgaaat cgaacctcat cgagtacacg ttcgacgggc gcgtggcgcg
     1921 cggctcccac gtgctcgagg tccgcgtcac ggacggcgtt ggcaaccggt cggtgtggcg
     1981 cgggccgttc acccgctgag cgccggcccg gcaggggaag tgctctcgag cagctcgagc
     2041 aggtcgtccg ccgacgcctc gcagcgtggg gcacgcatgt cgggaatccc gagggcggcc
     2101 agcgagtcgg gctccggaac ccggcgcccc acggcgcgct cgaccgtctc cgggaacttg
     2161 gccggatgcg cggtggccat gaccaggaac ggaccgcgca ggccttcctt cgcgagccgg
     2221 cgaacggctt ccaggccgac ggccgtgtgg ggatcgcaca cgtagcccgt ctcctcgaac
     2281 gtgcgccgca tggccccgat cgtgtcgtcg tccgataccg acaggccccg gatcccgcgt
     2341 cgcatgccct cggcgccgag cagcgcctcc aggcgttcga aattcgaggg cgcgcccaca
     2401 tccatcgcgt tcgaaagcgt ccggaccgac ggggccgccg gcctcggtcc gccctccagg
     2461 taacccggga aaccgcggtt cgcgttgtgg gcggcgacga gcgcacgcac cggcgccccg
     2521 gacaggcgtg ccagcacggc cgccgtcagg ttgcccaggt tgccgctcgg cacgacgagc
     2581 accgc
//
LOCUS       DBNHQZ010000356        11052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1461505, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000356 DBNHQZ010000000
VERSION     DBNHQZ010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1461505"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16YU_09605"
     CDS             <1..311
                     /locus_tag="AC16YU_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065522.1"
                     /translation="AGLVTVDAETGTELPEAVELSANWPNPFNPSTTIAYSLPRAETV
                     RLEVFDAAGRLVARLVDGLRPPGGHEATFDARHLASGVYLYRLRAGSTTRTRTMLLLK
                     "
     gene            386..3400
                     /locus_tag="AC16YU_09610"
     CDS             386..3400
                     /locus_tag="AC16YU_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR6065523.1"
                     /translation="MRRPTALALLTAVLLAVPFSASRAQDAASGAIEGTVHGVDADLL
                     QRVMVMAFDPDSSSGGMGHYLALPEPDGRYRIDGVDPGSYFVSAHSPEYGQQYFDGAA
                     YLSDAAPVTVAAGDLVGGIDFHLSLPGVGAGSISGVVLAAETGLPVAARVRVTSAATW
                     DLGFETTTDAEGKFRLDSLATGSYLVQAHAEGFVSEYFDDAIDYPTATPVLVQEPAET
                     AGIDFALDRAGSIAGMVLGLDGQPFEGAGVTAWSGSPWDSTGVVPPDSAWGDGGIGAD
                     GSWIGSWRGAFSAGTDADGRYRIVGLPPGSYVVSADGWTGWRSATVWYDGTQDPTLAT
                     PVDVQPGSAVEDIDFVLPLATGEGTIEGRVVDGSGDPVVGAYVNAVGNSNPAGMECCP
                     GWGGATTGQDGSYVLAGLPPGEYLVYAALYGNWTYLERWYPDAETPAEAEPVPVVRDQ
                     VTSGIDIVLPVDAYGASVSGIVSETGGEPLAWAQVLVQSLDDLTMPLDRVPIQAWAWT
                     DSSGAYTAHGLPAGTYVVSASWWDGDWYAHGWYDGAATLEEATPVALAEDEERTGVDI
                     ALDPEYWYGGIGGQVLDADGAAIERALVQVQPTSAVEGFAPFRFRDPHAVTDAEGRFV
                     IGRLAVGEYLLRAFADGAFAWYDAVPVADWATPVSVTGGDTTAVELELERRFDGPGLV
                     EGRVTVDGSVDWVMPAGAGSEDVPPIAVVTARPMVTILSWPESERFYPTVTREDGTYS
                     LGGLPVGEYVMRAFAPDHVEEFYDDAFDPSDADPVKVDGHLPATGIDFALIPMYWFGA
                     EDGAERAGMVGVHGAVVDESGEAVPGATVFLMNPDGVAVASTLTAADGTYALSGMMPG
                     SYRLMVAKEGFAAAFNGGAASLDGALPLDLSVGTVVVDLTLRVGGATDTEVGGDLPRT
                     YRLRGNYPNPFNPVTTVLVDLPETADVDVQVFDLLGRRVLDVSAGPTAAGDARPIQVD
                     ASGLASGMYLYRVRATGLASAWSAVGRMMLVK"
     gene            3474..3782
                     /locus_tag="AC16YU_09615"
     CDS             3474..3782
                     /locus_tag="AC16YU_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065524.1"
                     /translation="MRPVILPLLLAAVTGGFLSAPGPGRSPDRRGAPPHAPVRLAALE
                     EDTSCKIEFAGPGQQCGNYGTCPDTAHRSFFWGLIEYDTDVVLCFCLTECDIDITFWK
                     "
     gene            3779..4201
                     /locus_tag="AC16YU_09620"
     CDS             3779..4201
                     /locus_tag="AC16YU_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065525.1"
                     /translation="MTHSTTMPSRMADPALRYALALLLVLLGAESTVSMVRGWLTGSG
                     PSIATGFLMVVGGAGMLAGGRTLLGIAAWALRIQVVVTLLVGVMAAFGAPPGDGALPF
                     GPRWQPLLAGGLQVVVLVAALWWIARARSRNRGSTGSR"
     gene            4377..5138
                     /locus_tag="AC16YU_09625"
     CDS             4377..5138
                     /locus_tag="AC16YU_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR6065526.1"
                     /translation="MTRVRILAGLPLLALTLFGCAPSEPAMDARALVRAMHDRYADVR
                     PSSLTFVQQTILHRKPDAPPDTATWYEVAEPGRLRIDFAPVEEGNGALYRDGTLFAMH
                     GGRVAATRAEVNPLALLLMDVFQFEPERTVEMLDTLGFDLSALRETEWEGRPVWVVGA
                     PEGDETSAQFWVDRERLVAVRIIQPVGGGTRSWDTRVTGFREMAGYPQEERLVMYLDG
                     KLFQEESYRDIRAGIAVDPALFDTTDWIVEEPYWE"
     gene            complement(5250..5735)
                     /locus_tag="AC16YU_09630"
     CDS             complement(5250..5735)
                     /locus_tag="AC16YU_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR6065527.1"
                     /translation="MSQYTRIRTWCTALAAAAVLFTASPAVARDHSDDTASRLEAARV
                     LLESSAGALSSSQLTALTHTLSDALESTHFGFQTSALRLVIAYGDQVEFSHRTVIDVV
                     RLYRDHPDDRVRRMAVVALGTMDDDWGMDFLQRSLRFEKAGPVKHTIAAVLADDRAKV
                     G"
     gene            6002..7417
                     /gene="pyk"
                     /locus_tag="AC16YU_09635"
     CDS             6002..7417
                     /gene="pyk"
                     /locus_tag="AC16YU_09635"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6065528.1"
                     /translation="MNRRTKIVCTLGPASADAGTVRDLAVAGMDVARLNFSHGTHEDH
                     RRAIDLVREASAATGRTVAVMQDLQGPKIRVGRMPAKGIPLEVGQRLVLTSRAVPRSE
                     ASLVHIDYPTLADDVTEGSVILLDDGKLELRILEVRERDVVTEVVVGGTLHSRKGVNL
                     PNIRTSTPALTPKDLEDLSLGLELGVDFVALSFVRDESDVANLVSRVRESGHRTGIIA
                     KIEKPEAVERIDAIIEQSDGIMVARGDLGIEMRLSKVPGTQKRIIRKCQVAARPVITA
                     TQMLESMIENARPTRAEVSDVANAVLDGSDAVMLSGETAMGRHPVRVVEVMATIIEEA
                     ERHYREIGVIAPEDRSASDLTESVSFTAVQLATRVDAVAIACLTATGATARAIARHRP
                     RVPLYAFTDDRAVVGRLALTWGTKGFAVPFEYDTDKGIERVHKVLLEHGLARVGDRIV
                     ITAGMPLPTRGRTNMVHVSQL"
     gene            7545..>11052
                     /locus_tag="AC16YU_09640"
     CDS             7545..>11052
                     /locus_tag="AC16YU_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065529.1"
                     /translation="MALLAAALLQGCSAPGFVRRPVDNFRAYYNTFYNAKKAYTRGVE
                     GQPGAATTEAKVERLRYVSIFAAQREGRASTELEAAILKSADVLRDHPNSKWVDDALL
                     LIGKSYFHQRNLVGAEQKFREVIELESDLEDEARFWLARTLIAAERYGEAEAAIDEAL
                     SREDVAGRWESMLRLAKGDLRVQEQAWEDAALQLESGLEDVPEAEFGARAQYLLGQVL
                     ETLGRYPEAVRAYRDVGRFRPTYEWSYAAQFSAVRVEGLHVDARRALGRLRGMERDDK
                     NYAYRAELLYLRGLIAKSMGRADDAWRIFDDLLYDEAYVSLVGDLRGRVHYALGELYR
                     DLDRDFVLAAAHFDTAAAQLRSQLTRIGGAGSTTRGGTNRPDEGWAPEATLDTEEMKE
                     AFGAFAEVRGRIARMDSLLVLGALDDEAFERRILEIRQQIAREMEEERRRQEALAVQQ
                     AFARGASGTPDPSTGSRGNRPSGQNQQANVAGFLFHRDAVQAQEGLLSFQRTWGDRPL
                     VRNWRRIDAVSRLPEQELVDEFSDGAEREEERPEDVLPEVDVGAVPRDSTSLARMHAQ
                     RAAARYELANSLFLGIGRPDSAAHWYRLVLDESGEEDLSRRALFALAEVQQALGDSAS
                     ARAVYDRIVRDYPDSEFADRGRLRMGLPSERIEVPDSVAVAEEAYARQYEAWGRGEHR
                     WALDGMIHVAAEHAVLPVRPRSLLAAGLIFTEWAGIDSLDLLGPIPLRTSDSLLIDLG
                     LITPPVVDSVAADAGKPAVPEARRTGDAPGDAREAADDPERAAVDELDPEARRRRRID
                     DRAAPGTTRDRVADPDAMPPGVAANSAGVTVDSVGLAVDSTAAGLLGKDPDADRNSER
                     DGGVGADSLLAGADSLAGLGAIAMADSVRAGGIEAPEPPVDPDAWRVLTLEKLLAHIR
                     DRYGETPYAGRAGEMLAGLEEKKAQIARIADSLEALAALRAAAVSGQDPVAVAGDSLA
                     VQVDSLGLEGLPAAERPSAGAVYPDSIAAEADLPGVAMPPVVEPPPVEVREEEPAPDT
                     LAVDIDVPGVAEPAAPEPPPAEVEEQAPARDTLAVGVPAAISPVPGEAPAVDTTAAPG
                     ADEAAPPEGPATWRAVETSTARPLYDVESTLDTWLVVLEEVASPTMAPPVFRRFRETL
                     SGTGTIEMVTGTLDGEPVVYVVL"
BASE COUNT         1615 a         3720 c         4037 g         1680 t
ORIGIN      
        1 aggcggggct ggtgacggtg gacgcggaga ccgggacgga gcttcccgag gccgtggagc
       61 tctcggccaa ctggcccaac ccgttcaacc cgtcgacgac gatcgcatac tcgctcccgc
      121 gggccgaaac ggtgcggctc gaggtgttcg atgccgcggg acggctggtc gcgcggctcg
      181 tggacgggct caggccgccc ggggggcacg aggcgacctt cgacgcgcga cacctggcga
      241 gcggggtcta cctgtaccgc ctgcgggcgg gaagcacgac ccggacgcgg acgatgcttc
      301 tcctgaagtg accgcgggcc cggaatgaac gggcacatcg ggcggtctaa cgagtatccc
      361 gaaagaccgg cttacggaga gtgccatgcg tcgccccacc gccctcgccc tgctcactgc
      421 cgtcctgctc gccgttccgt tctccgcgtc ccgtgcccag gacgccgcat ccggagcgat
      481 cgagggcacc gtccacggcg tggacgcgga cctcctgcag cgcgtcatgg taatggcgtt
      541 cgatcccgat tcctcgtccg gcgggatggg ccactacctc gcccttccgg aacccgacgg
      601 caggtaccgc atcgacgggg tcgacccggg gagctacttc gtctccgccc attcgcccga
      661 atacgggcag cagtacttcg acggggcggc gtacttatcc gatgccgcgc ccgtgacggt
      721 cgccgccggc gacctcgtcg gaggcatcga ttttcacctg tccctcccgg gcgtcggcgc
      781 cggctccatc tccggggtgg tccttgcggc ggagaccggg cttccggtcg ccgcccgcgt
      841 gcgcgtcacc tcggcggcca cgtgggacct cggattcgag acgacgacgg acgcggaagg
      901 gaaattccgc ctcgacagcc tggccaccgg atcctacctg gtccaggccc acgcggaggg
      961 cttcgtatcg gagtatttcg acgacgcgat cgattacccg acggcgacgc ccgtcctggt
     1021 ccaggagcct gccgagacgg cgggcatcga tttcgcgctg gaccgggccg gatcgatcgc
     1081 cggaatggtc ctgggactcg acgggcagcc tttcgagggg gccggcgtga ccgcctggtc
     1141 gggctcgccc tgggactcca ccggggtcgt tccgcccgac tcggcgtggg gtgacggcgg
     1201 gatcggggct gacggatcct ggatcggttc gtggcgcggc gccttcagcg ccggcacgga
     1261 cgcggacgga cgctacagga tcgtcggcct gccgcccggc tcctacgtgg tgtccgccga
     1321 cggttggacc ggctggcggt cggcgaccgt ctggtacgac gggacccagg atccgacgct
     1381 cgcgacccct gtggacgtgc agcccgggtc cgcggtggag gacatcgact tcgtgctgcc
     1441 gctcgcgacc ggcgagggca ccatcgaggg ccgggtggtg gacggttcgg gcgatcccgt
     1501 cgtaggtgcg tacgtgaatg cggtcgggaa ctcgaacccg gccggcatgg aatgctgccc
     1561 gggctggggc ggcgctacga ccggccagga cgggagctac gttctcgccg gcctcccgcc
     1621 gggcgagtac ctggtatatg cggccctgta tggcaactgg acgtacctgg agcggtggta
     1681 tccggacgcg gaaacgccgg ccgaggcgga gccggtgccg gtcgtgcggg accaggtgac
     1741 atcgggcatc gacatcgtcc ttcccgtgga cgcctacggc gcctcggtct ccggcatcgt
     1801 gtcggagacc ggcggcgaac ccctggcgtg ggcgcaggtc ctggtccaga gtctcgacga
     1861 tctcaccatg cctctcgacc gcgttccgat ccaggcgtgg gcatggacgg actcctcggg
     1921 cgcgtacacg gcgcacggtc tgccggccgg cacgtacgtg gtctcggcct cctggtggga
     1981 cggcgactgg tatgcgcacg gctggtacga cggggcggcc acgctggagg aggcgacccc
     2041 cgtggcactc gcggaagacg aggagcgcac gggcgtcgac atcgcgctcg accctgagta
     2101 ctggtacggg ggcatcggcg gccaggtgct cgatgcggac ggcgctgcga tcgagcgggc
     2161 cctggtgcag gtccagccga cgagcgcggt cgagggattc gcgccgttcc ggttccgtga
     2221 tccgcacgcg gtcacggacg cggaggggcg gttcgtgatc ggacgcctcg ccgtcggcga
     2281 gtaccttctg cgtgcgttcg cggacggtgc tttcgcgtgg tacgacgcgg tgccggtggc
     2341 cgactgggcc accccggtgt cggtgacagg cggcgacacg acggccgtcg agctcgagct
     2401 cgagcgccgc ttcgacggac cgggcctcgt cgagggacgg gtcaccgtag acgggtcggt
     2461 cgactgggtc atgccggcgg gtgccggatc ggaagacgtg ccgccgatcg cggtcgtgac
     2521 cgcgcgaccg atggtcacga tcctcagctg gccggaatcc gagcgcttct acccgacggt
     2581 gacgcgcgag gacggcacgt attcgctcgg cgggcttccg gtgggcgagt acgtgatgcg
     2641 cgccttcgct cccgaccacg tggaggagtt ctacgacgac gctttcgatc cgtcggacgc
     2701 cgatcccgtc aaggtcgacg ggcacctgcc cgcgacgggc atcgacttcg cgctgattcc
     2761 gatgtactgg ttcggcgccg aggacggcgc ggagcgcgcc ggaatggtgg gcgtgcacgg
     2821 ggcggtcgtc gacgagtccg gcgaagcggt tccgggggca accgtgttcc tgatgaatcc
     2881 ggacggcgtc gcggtcgcct ccacgctcac ggccgccgac ggcacgtacg ccctgagcgg
     2941 tatgatgccg ggctcgtatc ggctgatggt ggcaaaggag ggcttcgcgg cagccttcaa
     3001 cgggggagcg gccagcctgg acggcgcgct tccgctcgac ctctccgtgg gaacggtagt
     3061 cgtcgacctg acgctccgcg tgggtggagc gacggacacc gaggtcggcg gggacctgcc
     3121 gcggacgtac cggctgcggg gcaactaccc gaatcccttc aacccggtga cgaccgtgct
     3181 cgtcgacctc cctgagacgg cggacgtcga cgtgcaggtc ttcgacctcc tcggaaggcg
     3241 cgtgctggac gtctcggccg gtccgacggc ggccggcgat gcgcgtccga tccaggtcga
     3301 tgcctcgggg ctcgcctcgg ggatgtacct gtaccgcgtc cgcgccaccg gactcgcctc
     3361 ggcctggagc gcggtcggcc ggatgatgct cgtgaagtga ccgggttatg acgggtcggt
     3421 ccgtcgggtc gcatggaacg agacgattcg ttccatccag acccccgacc cgcatgagac
     3481 ctgtcatcct tcccctgctg ctggccgccg tcaccggtgg cttcctgtcc gcccccgggc
     3541 cgggccgctc tccggaccgc cgcggcgccc cgccccatgc cccggtccgg ctcgccgccc
     3601 tggaggagga cacctcctgc aagatcgagt tcgccgggcc cggccagcag tgcggcaact
     3661 acggcacttg tccggacacc gctcaccgga gtttcttctg ggggctgatc gaatacgata
     3721 ccgacgtcgt gctctgcttc tgcctgacgg agtgcgacat cgacatcact ttctggaaat
     3781 gacgcacagc acaaccatgc cgtcccggat ggcggatcct gcgctacggt acgccctcgc
     3841 gctcctgctg gtactgctcg gcgccgaatc caccgtatcc atggtccgcg gctggctaac
     3901 gggctccggc ccttcgatcg ccacgggatt cctgatggtg gtcggcggcg ccggcatgct
     3961 cgccggcggc cgcactctgc tcgggatcgc cgcctgggcg ctccggatcc aggtcgtcgt
     4021 cacgctgctc gtcggcgtga tggcggcctt cggagcgcca ccgggcgacg gagcccttcc
     4081 gttcggcccc cgctggcaac cgctgctggc gggtggcctc caggtcgtcg ttctcgtggc
     4141 cgcgctgtgg tggatagcca gggccaggtc acggaatcgc ggatcgacgg gcagccggtg
     4201 agcgggcctg tggctcaccg ggaggggaag ctccggctcc gtggctcgag gacgtcgagg
     4261 gcgcgtaggc gctccggtta ccgtttcgct cccgtccgcc cggcgtcccg tgcatcccgg
     4321 ccccggatgg ggcgtatgcg gggcatcgat cccctgaacg gcaattgccg acccccatga
     4381 ctcgagtccg gatcctcgcg ggccttccgc tcctggccct gaccctgttc ggatgtgcac
     4441 cgtcggagcc ggccatggat gcccgggccc tcgtccgggc catgcacgac cgctacgccg
     4501 acgttcggcc gagctcgctc accttcgtgc agcagaccat cctgcaccgc aagccggacg
     4561 caccgcccga caccgccacg tggtacgagg tggccgagcc gggccggctc aggattgatt
     4621 tcgccccggt agaggagggg aacggcgccc tgtaccgtga cggcaccctg ttcgcgatgc
     4681 acggagggcg cgtggcggcc acgcgggcgg aggtgaaccc gctcgcgttg ctgctcatgg
     4741 acgtgttcca gttcgagccg gagcggacgg tggagatgct cgatacgctc ggattcgatc
     4801 tgtccgccct gcgcgagacc gaatgggaag gccgcccggt gtgggtggtc ggagcgccgg
     4861 aaggcgatga aacgtcggcc cagttctggg tggatcgcga gcgcctcgtg gcggtccgga
     4921 tcatccagcc cgtgggcggc ggaacgcgct cctgggacac acgggtgacg ggcttccggg
     4981 agatggccgg gtacccgcag gaggaacggc tggtgatgta cctcgacgga aagctcttcc
     5041 aggaggagag ctaccgggac atccgggccg gaatcgcggt ggatccggct ctcttcgaca
     5101 cgaccgactg gatcgtggag gagccgtact gggaatgacg ccacggacgg ctcccacgct
     5161 tccgcggccc gaagaaaggg aaaagccccg gccggagaac cggtcggggc ctttcacatg
     5221 gctccgggac cgtcaggccg ggtgagccgt cacccgacct tcgcccgatc gtcggcgagc
     5281 acggccgcga tcgtgtgctt aacggggccc gccttctcga accggagcga gcgctgcagg
     5341 aaatccatgc cccagtcgtc atccatcgtc ccgagcgcca cgacggccat gcgccgcacg
     5401 cggtcgtccg ggtggtcccg gtacaggcga acgacgtcga ttacggtccg gtggctgaac
     5461 tcgacctggt caccgtaggc gattaccagc cggagggccg acgtctggaa tccgaagtgg
     5521 gtggactcga gggcgtcgga gagcgtgtgc gtcagggcgg taagctgcga cgaggacagg
     5581 gcgccggcgc tgctttcgag caggacgcgg gcggcctcga ggcgcgacgc ggtgtcgtcg
     5641 gaatggtcgc gggcgacggc cggcgacgcg gtgaacagga cggcggcggc ggcgagcgcc
     5701 gtgcaccagg ttcggatgcg tgtgtactgt gacatgcctg gaccctccct cttggtttgt
     5761 ggcatggagg ggacggcgtg agatccgtcg aatccggcac ctgccggccc ggcgtgacgc
     5821 cgtgcccatg gactgattct ggtttgcggt acggtgcgtc cgatcgccgg atgcggatcc
     5881 gggccgaatc gtcgggatgg gcgcgtgagc ggtgcggcac gtcgcccgag tggcgcggtg
     5941 gccctggtca ttgccgcgcc gaccctcccc gcgtagtttg cgcccccgtt cacccgaggt
     6001 catgaaccga cgcacgaaga tcgtctgcac gctgggaccg gcctccgccg atgccggcac
     6061 cgtacgcgac ctggcggtgg cgggaatgga cgtcgcgcgc ctcaacttct cgcacggtac
     6121 gcacgaggac catcgccggg ccatcgatct cgtgcgggag gcgtcggcgg caacggggcg
     6181 gaccgtggcc gtgatgcagg acctccaggg tccgaagatc cgtgtggggc gcatgccggc
     6241 caagggcatt ccgctcgagg tcggccagag gctcgtgctg acgtcccggg ccgtcccacg
     6301 ctcggaggct tccctggtgc acatcgatta cccgacgctg gcggacgacg tgaccgaagg
     6361 ctcggtgatc ctcctggatg acgggaagct cgagctgcgc attctggaag tacgggagcg
     6421 ggacgtcgtg accgaggtgg tcgtgggcgg aacgctccac tcgcgaaagg gcgtgaacct
     6481 gccgaacatc cggacctcga cgcccgccct cacgcccaag gatctcgagg acctctcgct
     6541 cgggctcgag ctcggggtgg acttcgtggc cctctcgttc gtccgcgacg agtccgacgt
     6601 cgcgaacctg gtctcccgtg tccgggaatc gggccaccgg acgggcatca tcgcgaagat
     6661 cgagaagccc gaggccgtgg agcggatcga cgcgatcatc gagcagtccg acgggatcat
     6721 ggtggcccgc ggggacctgg gcatcgagat gcggctgtcc aaggttccgg gcacgcagaa
     6781 gcgcatcatc cgcaagtgcc aggtggcggc gcgtccggtc atcaccgcca cccagatgct
     6841 ggagagcatg atcgagaacg cccgccccac gcgcgccgag gtcagcgacg tggccaacgc
     6901 ggtactggac ggatccgacg ccgtgatgct ctccggcgag accgcgatgg gacgtcaccc
     6961 cgtccgggtc gtcgaggtca tggcgacgat catcgaggag gccgagcggc actaccggga
     7021 aatcggtgtg atcgctcccg aggaccggtc ggcatccgat ctcacggagt ccgtcagctt
     7081 cacggccgtg cagctcgcca cgcgcgtgga cgccgtcgcg attgcctgcc tcacggccac
     7141 cggtgccacg gcccgcgcga tagcccggca ccgtccgcgc gtcccgctgt acgcgttcac
     7201 cgacgatcgg gcggtggtcg gacgcctcgc cctcacgtgg ggaacaaaag gatttgcggt
     7261 tccttttgaa tacgacacgg acaagggcat cgagcgcgtg cacaaggtgc tgctcgagca
     7321 cgggcttgcc cgtgtgggtg accgcatcgt gatcacggcg ggcatgcccc tgcccacgcg
     7381 tggtcgtacc aacatggtgc acgtgagtca gctctgatcc accgaaacaa gagacacgga
     7441 tcgggattcc gctacggccc aacactccga acccagtacg ctccgtacgc cccgtggccg
     7501 accggtatcc acgttcgccc gccgcacgct tcggcatcct tccggtggcg ctgcttgcag
     7561 cggcgctcct ccaggggtgc agcgctccgg gattcgtcag gcggcccgtc gacaacttcc
     7621 gggcgtatta caacaccttt tacaacgcga agaaggcgta cacgcgcggg gtggaagggc
     7681 aaccgggcgc cgcgaccacg gaggccaagg tcgagcggct caggtacgtg agcatattcg
     7741 ccgcccagag ggaaggacgg gccagcaccg agctcgaggc cgcgatcctc aagagcgccg
     7801 acgtcctgcg cgatcaccca aactcgaagt gggtcgatga cgcgctcctc ctgatcggga
     7861 agtcgtactt ccaccagcgg aacctggtgg gggccgagca gaaattcagg gaggtcatcg
     7921 agctcgaaag tgacctcgag gacgaggcca ggttctggct ggcgcgcaca ctcatcgctg
     7981 ccgagcgcta cggcgaggcg gaggccgcga tcgacgaagc gctctcgcgc gaggatgtcg
     8041 ccggccgttg ggagtcgatg ctgcgcctgg ccaagggcga tctgcgggtg caggagcagg
     8101 cctgggagga cgccgcgctg cagctcgagt cgggactcga ggacgtgccc gaggcagaat
     8161 tcggagctcg tgcccagtac ctgctcggcc aggtgctcga gaccctcggg cggtatcccg
     8221 aggcggtccg tgcctaccgg gacgtcggcc gcttccgccc cacgtacgag tggtcgtacg
     8281 cggcacagtt cagcgcggtc cgcgtcgagg ggctgcacgt ggacgcccgg cgcgcactgg
     8341 ggaggcttcg gggcatggag cgggacgaca agaactatgc gtaccgggcc gagctcctgt
     8401 acctgcgcgg gctcatcgcg aagtccatgg gccgggccga cgatgcctgg cgcatcttcg
     8461 acgacctcct ctacgacgag gcctacgtgt cgctcgtggg cgacctgcga ggccgggtcc
     8521 actacgcgct cggcgaactg taccgtgacc tggaccggga cttcgtcctc gcggcggccc
     8581 atttcgacac ggcggcggcc cagcttcgca gccagttgac ccggatcggg ggagcaggct
     8641 ccacgactcg gggaggcacg aaccggccgg acgagggctg ggctcccgag gccacgctcg
     8701 acaccgagga aatgaaggaa gcgttcggag ccttcgcgga ggtgcggggc cggatcgctc
     8761 ggatggactc gctgctcgtg ctcggggccc tcgacgacga ggcgttcgag aggcggatcc
     8821 tggaaatccg ccagcagatc gcccgcgaaa tggaggagga gcggcggcgc caggaagctc
     8881 tggccgtcca gcaggctttc gcgaggggcg cttcgggcac cccggatccg tcgaccggat
     8941 cccgcggcaa ccgtccgtcg gggcagaacc agcaggcgaa cgtggccggt ttcctgttcc
     9001 accgggatgc cgtgcaggcc caggaggggc tgctcagctt ccagcggacg tggggtgacc
     9061 gccccctggt gcgcaactgg cggaggatcg acgcggtctc gcggctgccc gagcaggaac
     9121 tggtcgacga gttttccgac ggtgccgagc gcgaggagga gcggccggag gacgtgcttc
     9181 cggaggtgga cgtgggcgcc gttccgcgag actcgacctc cctcgcccgc atgcacgcgc
     9241 agcgcgccgc ggcccgatac gagctggcga actcgctctt cctgggcatc ggacgccccg
     9301 actcggccgc gcactggtac cggctggtcc tggacgaatc cggggaggag gacctgtccc
     9361 gccgcgcact cttcgccctg gccgaggtgc agcaggcgct gggagactcg gcctcggccc
     9421 gcgccgtcta cgaccgcatc gtacgcgact accccgactc cgaattcgcc gaccgcggac
     9481 gcctgcggat gggtctcccg tcggaacgga tcgaggtacc ggactccgtg gcggtcgccg
     9541 aggaggccta cgcccggcag tacgaggcgt gggggcgcgg ggagcaccgc tgggctctcg
     9601 acggcatgat ccacgtggcc gccgagcacg cggtgctccc ggtcaggccc cggtccctgc
     9661 tcgcggccgg cctgatcttc accgagtggg cgggcatcga ttcgctggac ctgctgggac
     9721 cgatcccgct gcgaacgagc gactccctgc tcatcgacct gggactcata acgcctcccg
     9781 tggtcgattc cgtcgcggcc gatgccggga agccggctgt cccggaggcc aggcggaccg
     9841 gggatgcgcc gggcgatgcc cgggaggccg cggacgaccc cgagcgcgcg gccgtggatg
     9901 agctggatcc ggaagcccgc aggcggcggc gcatcgacga ccgggcggca cccggtacga
     9961 cccgggaccg ggtggcggac ccggacgcca tgccgcccgg cgtcgcagcg aattccgctg
    10021 gcgtgaccgt cgattccgtc gggttggccg tcgattccac ggccgccgga ctgcttggga
    10081 aggaccccga tgcggaccgg aattccgaac gggatggcgg cgtcggcgcg gattctctgc
    10141 tcgccggtgc cgattccctg gcgggcctcg gggccattgc gatggcggac tccgtacgtg
    10201 cgggcgggat cgaggcgccg gagcccccgg tcgatccgga tgcgtggaga gtactgaccc
    10261 tggaaaagct cctggcgcac atccgcgacc ggtacggcga gacgccctac gccgggcgtg
    10321 cgggcgagat gctggccggc ctggaagaga agaaggcgca gatcgcacgg atcgcggatt
    10381 cgctcgaggc gctcgcggcc ctgcgtgccg cggccgtttc gggacaggat ccggtggccg
    10441 tcgccggcga ttctctcgcg gtacaggtcg attcgctcgg gctggaaggg ctccccgccg
    10501 cggagcggcc gtcggccggg gcagtatacc cggactcgat cgccgcggaa gccgacctgc
    10561 cgggcgtggc catgccgccg gtcgtggagc cgcccccggt cgaagtccgg gaggaggagc
    10621 ctgcgccgga tacgctggcc gtggacatcg atgtgccggg cgtggccgag cccgccgccc
    10681 cggagccgcc gcccgccgag gtggaggagc aggcgccggc gcgggacacg ctggcggtcg
    10741 gggttccggc ggcgatttcc cctgtgcccg gcgaggctcc ggcagtggac acgaccgcgg
    10801 cgcccggtgc ggatgaggcc gccccgcccg agggtcctgc cacgtggcgc gcggtcgaga
    10861 cctcgacggc ccggccgctc tacgacgtgg agagtacgct cgacacgtgg ctcgtggtgc
    10921 tcgaggaggt ggccagtccc accatggcgc cacccgtctt ccgccgcttc cgggagacac
    10981 tttccggcac gggaacgatc gagatggtga ccgggacgct cgacggcgag cccgtcgtgt
    11041 acgtcgtgct cg
//
LOCUS       DBNHQZ010000357         5061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1461656, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000357 DBNHQZ010000000
VERSION     DBNHQZ010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1461656"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16YU_09645"
     CDS             <1..231
                     /locus_tag="AC16YU_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065530.1"
                     /translation="YPRYFRFDLGVTKQVSVFERGAGAPVTLALTAELLNLFDMTNTV
                     AYSWIPNQSGIWTRVPTRLTPRTLNVGARVDF"
     gene            complement(203..1189)
                     /locus_tag="AC16YU_09650"
     CDS             complement(203..1189)
                     /locus_tag="AC16YU_09650"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065531.1"
                     /translation="MRLAALVPVLVVLAACAEPAPPVEPHVPDAEAISLFGTPLLRQP
                     LPEEARLRLEADLAAAEATLREDSTSVDAIVWVGRRQAYLGRYRDAVATFTRGLELHP
                     DEPHLLRHRGHRRITLRQFGAAIADLSRAAELTMDRPDEVEPDGQPNAAGIPTSTLQT
                     NIWYHLGLAHYLLGRFEAAETAFGRCLELAGNDDMRVAATDWVYMALRRQGRDAEAAA
                     LAAGIRPDLELLENHAYHRRILFYRGLLGVDSLLSAPADGDSALTIATQGYGVGNWYL
                     VEGDTLRAAELLRRVIETRSWAAFGYIAAEADLVQLGDAAAVPVRNPPEPPR"
     gene            1513..3810
                     /locus_tag="AC16YU_09655"
     CDS             1513..3810
                     /locus_tag="AC16YU_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6065532.1"
                     /translation="MKRNSLLFLLLIASAASVRAQSGTVTGLVTDAQTKQPLIGANVL
                     LVGTTQGAATDLDGNYVITGVPAGTFALRFSYLGFDTRTETDVVVRPDRVTRVEVGLS
                     MSAIESAAVVVNAGYFPRSDAQPVSLARFTREEVRRAPGSAGDVSRILFALPSVAKGN
                     DTMNSLVVRGGTSFENAFYLDNIEIPNINHFPEQGATGGPIGMVNVEFIDDVTFNAGG
                     FSALYGNRLSSVTDLSLREGNREEVDLQLDLNMAGIGGLAEGPLGRKGSWMLSARRSF
                     LDLIVGAIGEMDAIPQYGDVQGKVTVDLSPDHRLTAVGLLGLDAIGMDRDKAIENGTN
                     VYANADISQGTLGVNWRWLWSGSGFSNTSVSVSNVDYDRVARDTREYMETGEEHVLLD
                     LVGFERSWKLRNVNHLRLGGAARIEFGVDARIDQVDFDHRYGRYDDVLGQELEPLRAT
                     DRSSNARVAGFASLTLRPVGSVAVTPGVRVAYADLTGRTEVDPRLSARVDLSARTAVT
                     GAVGLYHQELPLFLLAQAPGNRDLATPRAVHVVLGVSHLLAPETRLTIEAYDKQYHDM
                     PADPTQPQLYLLDEVARYGTLIHHPPLVDGARARARGVEVLVQKRLAERLYGHASVGM
                     SRAEYRGLDGEWRARSVENRFMAHVEGGYKPNRNWEFSARWIVASGAPFTPFDEEASA
                     AARRAVLDASRINDSRMPTYQSLNIRFDRRHYFGWGNMVTYLSIWNAYAHENVAAYVW
                     NEVDNAPEGMSGWGMLPVFGVEVEF"
     gene            3899..5032
                     /locus_tag="AC16YU_09660"
     CDS             3899..5032
                     /locus_tag="AC16YU_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6065533.1"
                     /translation="MPIPTGLFSSILVLGVGQGVLLALLLLRRSRSAAHTLLAALMLA
                     YAVYLAQELLIAAGLDLQYPRWIALLAATPLLFGPLHYLFARYLTAPDRPFRAVTWLN
                     FLPFVLLRISMLPVILGDRATLVEFMERERSGALEPAGIVIGGLVAVHGLTYMILTVR
                     LLDRVRNDRSQDADRLKWLRQMTWLALFVWVVVGIEMALRPFGIDISQTAPSPVGLLT
                     GIVVYATGYLGLRRAGNFLVDVRRFAGDSPSAPPYSRSGLSPQAARRYLAELVALMER
                     EKPFRDSTLTLQELADRLDVSRHHLSEVLNAERGESFFDFVNGYRVDEVKRMLADRSY
                     ENLTLMAIAEEAGFSSKSTFNDSFKRITGMTPSEYLERPDGRR"
BASE COUNT          756 a         1785 c         1738 g          782 t
ORIGIN      
        1 tacccccgct acttccggtt cgacctcggg gtcaccaagc aggtctcggt cttcgaacgg
       61 ggggccggcg ccccggtgac cctcgcgctc acggcggagc tgctgaacct cttcgacatg
      121 acgaacacgg tcgcgtactc gtggattccg aaccagtcgg gcatctggac ccgcgtcccg
      181 acccgcctga ctccgaggac gctcaacgtg ggggctcggg tggatttctg acgggcacgg
      241 ccgccgcgtc ccccagctgc acgaggtccg cctcggccgc gatgtatccg aacgccgccc
      301 acgaccgggt ctcgatcacg cggcggagaa gctcggccgc acgaagcgtg tcgccctcga
      361 ccaggtacca gttgcccacg ccgtatccct gggtggcgat cgtgagggcc gaatccccgt
      421 cggccggggc ggagagcagc gagtcgaccc cgaggagtcc ccggtagaac aggatgcgcc
      481 ggtggtaggc gtggttctcg agcagttcca ggtcgggccg gatcccggcc gcgagggcgg
      541 cggcctccgc gtcccgcccc tggcgccgga gcgccatgta cacccagtcc gtcgcggcga
      601 cgcgcatgtc gtcattgccg gcgagctcca ggcagcgccc gaacgcggtc tcggccgcct
      661 cgaagcgccc caatagatag tgcgcgagcc ccaggtgata ccagatgttg gtctgcagcg
      721 tgctcgtcgg gatgccggcg gcgttgggct gcccgtccgg ctcgacctcg tccggccggt
      781 ccatcgtgag ctccgccgcc cgggacaggt cggcgatggc cgcgccgaac tgccgcagcg
      841 tgatcctccg gtgaccccgg tggcgcagca ggtgcggctc gtcggggtgc agctccaggc
      901 cgcgcgtgaa cgtcgcgacg gcatcccggt agcgccccag gtacgcctgc cgccgtccga
      961 cccacacgat cgcgtcgacc gacgtggagt cctcgcgcag ggtggcctcg gcggccgcga
     1021 ggtccgcctc caggcgcagg cgcgcctcct ccgggagcgg ctggcgaagc aggggcgtgc
     1081 cgaagagcga gatggcctcc gcgtcgggca cgtggggctc caccggcgga gcgggctcgg
     1141 cgcacgcggc gaggacgacg aggaccggga cgagggcggc gaggcgcatg gcgtggctcg
     1201 gcaggtggcc cccgaatgta tggggacgga caataaggcg cgacggtgca ctgagaaggt
     1261 ccggatgctt cggggacgac cggcccggtc ggcccggacg ccctccgggc ggagtccacg
     1321 taggcctgtg gcagataacg gaccctccgc atccgccgca atgaactgga ccggcatcag
     1381 actctggctc gccgtggggg cgctcgcagc agcgacgcgc agcctcgcct ggctcagcca
     1441 acggttcgag gcctggaccg ggcggctggt gcggatctcc gttcgactcc tcgaacgaac
     1501 ccggaacctg ccatgaaacg caacagcctg ctcttcctgc ttctcatcgc ctccgcggcc
     1561 tccgtccgcg cccagtccgg gaccgtgacg ggactcgtga cggacgccca gaccaaacag
     1621 ccgctcatcg gcgccaacgt gcttctcgtg ggaacgaccc agggagccgc caccgatctc
     1681 gacggcaact acgtgatcac gggcgtgccc gccggcacgt tcgccttgcg cttctcctat
     1741 ctgggcttcg acacacggac cgagacggac gtggtggtcc ggccggaccg ggtgacgcgc
     1801 gtcgaggtcg ggctctcgat gtccgcgatc gagtcggccg ccgtggtcgt gaacgcgggg
     1861 tatttcccgc ggagcgacgc acagccggtc agcctggcgc ggttcacccg tgaggaggtc
     1921 cgccgcgccc cgggctcggc cggcgacgtg agccgcatcc tcttcgccct gcccagcgtg
     1981 gccaagggca acgacacgat gaacagcctc gtggtcaggg gcgggacgtc cttcgagaac
     2041 gcgttctacc tggacaacat cgagatcccg aacatcaacc acttcccgga gcagggcgcg
     2101 accggggggc cgatcgggat ggtcaacgtc gagttcatcg acgacgtcac cttcaacgcc
     2161 gggggcttct ccgccctcta cggcaaccgc ctctcctcgg tcacggacct gtccctgcgc
     2221 gagggcaacc gggaagaggt cgacctgcag ctcgacctga acatggccgg catcggcggg
     2281 ctcgccgagg gccccttggg ccggaagggc tcctggatgc tgtcggcgcg ccgcagcttc
     2341 ctggacctga tcgtgggcgc gatcggcgag atggatgcga tcccgcagta cggcgatgtc
     2401 cagggcaagg tcaccgtgga cctgtctccc gaccaccggc tgaccgccgt gggcctcctc
     2461 gggctcgacg cgatcgggat ggaccgcgac aaggccatcg agaacggcac gaacgtctac
     2521 gctaacgccg acatcagcca gggaaccctc ggcgtgaact ggaggtggct ctggagcggg
     2581 agcgggttct cgaacacgtc ggtctccgtg tccaacgtgg actacgaccg cgtggcgcgg
     2641 gacacccggg agtacatgga gaccggggaa gagcacgtcc ttctcgacct cgtcgggttc
     2701 gagcgctcgt ggaagctgcg caacgtgaac cacctgcggc tcggcggtgc ggcccggatc
     2761 gagttcggag tcgacgcgcg gatcgaccag gtggacttcg atcaccggta cggccgctac
     2821 gatgacgtcc tcgggcagga gctcgagccg ctccgggcga ccgatcgatc ctcgaacgca
     2881 cgcgtggccg gattcgcaag cctgacgctc cgcccggtcg gctccgtcgc ggtgaccccg
     2941 ggcgtccggg tcgcgtacgc ggacctgacc ggaaggacgg aggtcgatcc ccggctctcg
     3001 gcccgcgtgg acctgtccgc ccggaccgcc gtcaccggcg ccgtgggcct gtatcaccag
     3061 gagcttccgc tcttcctgct cgcgcaggca cccgggaacc gcgacctcgc gacgccccgc
     3121 gccgtgcacg tggtcctggg agtgagccac ctcctggccc cggagacccg cctcaccatc
     3181 gaggcctacg acaagcagta ccacgacatg cccgcggacc cgacgcagcc gcagctctac
     3241 ctgctcgacg aggtcgcgcg ctacggcacc ctgatccacc acccgcccct cgtcgacggg
     3301 gcgcgtgcca gggcacgcgg cgtcgaggtc ctggtgcaga agaggcttgc cgagaggctg
     3361 tacgggcacg cgagcgtggg gatgagccgc gcggagtacc ggggcctcga cggcgagtgg
     3421 cgtgcccgca gcgtcgagaa ccggttcatg gcccacgtcg agggcggcta caagccgaac
     3481 aggaactggg agttcagcgc acggtggatc gtggcgtcgg gggccccctt cacgccgttc
     3541 gacgaggagg cctcggccgc cgctcggcgc gcggtgctcg acgcctcgcg catcaacgac
     3601 tcacgcatgc ccacgtacca gtccctgaac atccgcttcg accgcaggca ctacttcggg
     3661 tggggcaaca tggtcaccta tctatcgata tggaacgcct acgcccacga gaacgtggcg
     3721 gcctacgtgt ggaacgaggt cgacaacgcg cctgaaggca tgagcggatg gggcatgctg
     3781 cccgtgttcg gcgtggaagt cgaattctga cgggcgtcgt ccccggacgt gaagcctccg
     3841 gcgaaagacg gcgtacaagg gcgatcccgc ttgcggggtc gcccttttcg cgtctccgat
     3901 gcccatcccg accggcctct tctcctcgat cctcgtgctg ggcgtcggcc agggcgtgct
     3961 gctggccctg cttctgctgc gccggtcccg ctcggcggcc catacgctgc tggccgcgct
     4021 gatgctcgcc tacgccgtgt acctggcgca ggagctcctg atcgcggcgg gcctcgacct
     4081 gcagtatccc cgctggatcg ccctcctcgc cgcgaccccg ctcctgttcg gccccctgca
     4141 ctacctcttc gcgcggtatc tgaccgcgcc cgatcggccg ttccgcgcgg tcacctggct
     4201 gaactttctt ccgttcgtcc tcctgcggat cagcatgctg ccggtgatcc tgggcgaccg
     4261 ggccaccctc gtcgaattca tggagcgcga gcgctcgggc gcgctcgagc cggccgggat
     4321 cgtcatcggg ggcctcgtcg cggtgcacgg cttgacgtac atgatcctca cggtgcgcct
     4381 gctcgaccgc gtccggaacg atcgcagcca ggacgccgac cgtctgaaat ggctgcggca
     4441 gatgacctgg ctggcgctgt tcgtttgggt ggtcgtcggc atcgagatgg ccctcaggcc
     4501 cttcggcatc gacatatccc aaaccgcgcc gagccccgtc ggcctcctga ccgggatcgt
     4561 ggtctatgcg accggctacc tggggctgcg ccgggcgggc aacttcctcg tcgacgtccg
     4621 ccgctttgca ggcgattcgc cgtccgcgcc cccgtattcc cgctcggggc tctccccgca
     4681 ggccgctcgc aggtatctcg cggagctcgt cgcgctcatg gagcgggaga agcccttccg
     4741 ggacagcacc ctcacccttc aggagctcgc cgatcggctc gacgtgtcgc gccatcacct
     4801 gagcgaagtg ctgaacgcgg agcggggcga gagcttcttc gacttcgtga acgggtaccg
     4861 ggtggacgag gtcaagcgga tgctcgcgga ccgttcgtac gagaacctca cgctcatggc
     4921 catcgcggaa gaggcgggtt tctcgtcgaa gtcgaccttc aacgacagct tcaagcggat
     4981 tacgggcatg acgccgtctg aatacctgga gcggccggat ggcaggcgtt gaggcccttc
     5041 cgtccggccc ggcagacggt g
//
LOCUS       DBNHQZ010000358         5555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3628841, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000358 DBNHQZ010000000
VERSION     DBNHQZ010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3628841"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16YU_09665"
     CDS             <1..195
                     /locus_tag="AC16YU_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6065534.1"
                     /translation="RSMVSTGDRSAKIRTYNWPQGRVTDHRLEGEDKNHSLERVMEGN
                     LDGIIRALRTAENADRLAKL"
     gene            347..712
                     /gene="rpsF"
                     /locus_tag="AC16YU_09670"
     CDS             347..712
                     /gene="rpsF"
                     /locus_tag="AC16YU_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6065535.1"
                     /translation="MGIDKNTYELTYIVNAVLSDDQIKDIVQRVTQYIAEGGGEIAEV
                     DEWGSRRLAYPLQKKRNGYYVNMYFRAPGAFIPRLERALEIDDNILRYLTLRLDSKMQ
                     RHYEERSKQRASEKAAAAN"
     gene            724..945
                     /gene="rpsR"
                     /locus_tag="AC16YU_09675"
     CDS             724..945
                     /gene="rpsR"
                     /locus_tag="AC16YU_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6065536.1"
                     /translation="MRDDSVSTEEKFLEPAYVDYKDVELLKRFLNEQGKLLPRRITNV
                     SARFQRQLTTAVKRARHLALIPFVADAVR"
     gene            993..1562
                     /gene="rplI"
                     /locus_tag="AC16YU_09680"
     CDS             993..1562
                     /gene="rplI"
                     /locus_tag="AC16YU_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR6065537.1"
                     /translation="MKVILLQELDNLGDEGDIVTVKDGYGRNFLIPRGLAKVATTGAV
                     KAWQEERRQASRKLAQRKEDAERLAAELSRIELVVTAKVGAENRIFGSITSQQIVEAL
                     AHEGVTVDRRKVEMTEDVRHIGVYTAEVKLHPEVVAQVKVRVEPEGGVAAAPEVPAPE
                     SPAPETPVEEPAPADDAGPDFDLFEDDEE"
     gene            1646..3637
                     /locus_tag="AC16YU_09685"
     CDS             1646..3637
                     /locus_tag="AC16YU_09685"
                     /EC_number="1.8.-.-"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HIR6065538.1"
                     /translation="MAIARALLFLLVVALPNAEALAQFEPVGPATDYVSWTARIEPSR
                     VAPGDTAYLVLDATVAQPWKVYALDASLPPLSDGPRPYGVDVVWDEWPQGIAAAGAPE
                     QSAPEIGHDPNFDKTLGWFHGTARFAWPLAVSVDAPAGEVTVTGSVRFQICNDDRGIC
                     LPPTPVPFAAVVVADPECSDGTTGEDAGCELDDAAFGEMMGSGPEPGESPGGQRPAPV
                     SRSMLGFLLLALGAGLASLLTPCVFPMVPLTVSYFTKHADNRGEAVRMAAVFGGAIVV
                     TFTLLGILMAALVGAAGAQTIAANPWVNLFIALVLIGFALSLLGLYELRVPNALLNFT
                     NRQSTQRGGAVGVLFMGLTLTLVSFSCTAPFVGGLLAAAAGGTWAWPMLGMVVYSAAF
                     ALPFVLLALFPRALSTLPKSGAWMNVLKVVLGFVELAASLKFLSNADLVWGWGILSRP
                     LAIAITVVILFVAGVYLLGKLRLSHEEPAPAVGVGRLLAGVGFIAVSLYMLPGLFGAP
                     LNSLDAFLPPRQGTDVSLLAVLGPGSVPRDDEEWIVDDIDAAVDFSGVSNRPLFVDFT
                     GYTCTNCREMEANVFPAAPVRERLGRDFVLLRLYTDGLERGTEFQRYQLQLTGTVALP
                     TYAVLAADGRTLLAVESGIMSRDAFVRFLDGASSAFSRL"
     gene            complement(3649..4113)
                     /locus_tag="AC16YU_09690"
     CDS             complement(3649..4113)
                     /locus_tag="AC16YU_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6065539.1"
                     /translation="MTILFAVDLSEPADVTSSVEEIALRQGADLCVLHVYQPSPASPM
                     PVDPMTGFGDMAYAAYDPTVQASMEQAEQHEFDAFVRERFRRVVRPALLQGDPARTIL
                     QEAERLDADLIVVGKRHHGMLERFLLGSVSQAVVNQAHRPVLVVPVSGPRGD"
     gene            4230..4871
                     /locus_tag="AC16YU_09695"
     CDS             4230..4871
                     /locus_tag="AC16YU_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065540.1"
                     /translation="MPRSTPSRLHWPGPATGEPVPAVLLQRAQHGDREACHRLLGWAY
                     RSACDYYVGKVGVEVLLSPTDAEELAGMFLLEFERAWPRIRSLGHYTRRMLRNNLRRY
                     LRRKRELRRREAPYASHEMDRIPDAALHVSESHPIERWGDDEAAQFAAVRETLSRTDP
                     LTRDLMALRSGDPPLSYAVIAVRLNTTETALRMRAARFYQAVRDTHRAMAGPG"
     gene            4907..5305
                     /locus_tag="AC16YU_09700"
     CDS             4907..5305
                     /locus_tag="AC16YU_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065541.1"
                     /translation="MPIDSGYEDGTEPLSVECIHRWVRLAAVTRASEDAGSAGARLAM
                     VAACIVCLTLGATAREITHWALGDTSFGPFSARQTPTDPWFRCGGGFAPGRAEEAWRE
                     PSGWAPSDDAFALPIPGFRTGPVNQEGGTP"
     gene            5347..>5555
                     /locus_tag="AC16YU_09705"
     CDS             5347..>5555
                     /locus_tag="AC16YU_09705"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6065542.1"
                     /translation="MPHFLDTPVTLLLLVVNVLVSGYALYGDEGLLDRLSFRPRQIIR
                     DREYYRLVTAGFVHGGFGHLAVNMLT"
BASE COUNT          874 a         1861 c         1910 g          910 t
ORIGIN      
        1 cgcagcatgg tctcgaccgg cgaccgctcc gccaagatcc ggacctacaa ctggccccag
       61 ggtcgcgtca ccgaccaccg tctggagggc gaggacaaga accactcgct cgagcgggtg
      121 atggagggaa acctggacgg gatcatcagg gcgctccgga ccgcggagaa cgccgaccgc
      181 ctggcgaagc tgtgaggatg gccaccgggc ggtgcagatg ccgcaaagaa ggggacgcat
      241 atggacaatc gtccctcttc gacgtattat tataggctcg cgattaatcc ctgctccgcg
      301 tcgcacgacc cggggcataa gccaatccga ttccacaggg agagcgatgg gtatcgacaa
      361 gaacacgtac gagttgacgt acatcgtcaa cgccgtcctc agcgacgacc agatcaagga
      421 catcgtccag cgcgtcacgc agtacatcgc cgagggcggt ggcgagattg cggaagtcga
      481 tgagtggggc agccggcggc tggcctaccc gctgcagaag aagcgcaacg ggtactacgt
      541 caacatgtat ttccgcgcgc ccggggcgtt catcccccgg ctcgagcgcg cgttggagat
      601 cgacgacaac atccttcgct acctcacgtt gcgcctggat tccaagatgc agcgtcacta
      661 cgaggagcgt tcgaagcagc gggcgtccga gaaggccgcc gcggcgaact gagggccacg
      721 accatgagag atgattccgt ttcgaccgaa gagaagttcc tggagccggc ctacgtcgat
      781 tacaaggacg tggaactgct caagcgtttc ctgaacgaac agggcaagct gctgccccgc
      841 cggatcacga acgtgtcggc ccgcttccag cggcagctga ccaccgcggt caagcgcgcg
      901 cggcacctgg cccttattcc cttcgtcgcg gacgccgtcc gctgagctgg cgatgaatct
      961 ccggccgtgg ccggacaggc ataccaagcg tcatgaaagt catactgctc caggaactcg
     1021 acaacctcgg cgacgagggg gacatcgtca ccgtcaagga cgggtacggt cgcaattttc
     1081 tcattccgcg cggcctggcc aaggtggcca cgacgggcgc cgtgaaggca tggcaggagg
     1141 agcgccggca ggcctcgcgc aagctcgcgc agcgcaagga ggacgccgag cgcctcgccg
     1201 cggagctctc ccggatcgag ctggtcgtca cggcgaaggt cggcgcggag aaccgcatct
     1261 tcggatcgat cacgtcccag cagatcgtgg aagcgctggc ccacgaaggg gtgaccgtcg
     1321 atcgccgcaa ggtcgagatg acggaagatg tccgtcacat cggcgtgtac acggccgagg
     1381 tcaagctgca tcccgaagtc gtggcgcagg tcaaggtgcg ggtcgagccc gagggcggcg
     1441 ttgcggcggc gcccgaggtt ccggcgcccg agtcgcccgc gccagagaca cccgtggagg
     1501 agccggctcc ggcggacgac gccggcccgg atttcgatct gttcgaggac gacgaggagt
     1561 aatccgggcc gtcgtcgccg ggcacaattc ggaagggtct tcgttagccg ggcgctgacg
     1621 gagacccttt cgcattcggg caaccatggc gatcgcacgc gccctgctat ttctcctggt
     1681 cgtcgcgctt ccgaacgcgg aggcgctggc ccagttcgag ccggtcggcc cggcgacgga
     1741 ctacgtgtcg tggacggccc ggatagagcc ctcacgggtc gcgcccggcg ataccgcgta
     1801 tctcgtgctc gacgcgaccg tcgcgcagcc gtggaaggtg tacgccctcg acgcgtccct
     1861 tccgcccctg tccgacggac cccggcccta cggggtggac gtggtatggg acgaatggcc
     1921 ccagggcatt gccgcggccg gagcgccgga gcagtccgcc ccggaaatcg ggcacgatcc
     1981 caacttcgac aagaccctgg gctggtttca cgggacggcg cggttcgcgt ggcccctcgc
     2041 cgtgagcgtg gatgcgccgg caggcgaggt caccgtgacc ggctccgtgc ggttccagat
     2101 ctgcaacgac gaccgcggga tctgtctccc gccgacgccc gtgccgttcg ccgccgtcgt
     2161 cgtggcggat ccggagtgtt ccgacgggac gaccggcgag gacgcgggct gcgagctgga
     2221 cgatgcggcc ttcggcgaga tgatgggttc cggcccggag cccggggagt ccccgggcgg
     2281 gcagcgcccg gctcccgtct cacgcagcat gctcggcttc ctgctcctgg cgctcggcgc
     2341 gggcctggca tcgctcctca caccgtgcgt cttcccgatg gtcccgctca cggtctcgta
     2401 cttcacgaag catgcggaca accgggggga ggccgtccgc atggccgccg tcttcggggg
     2461 cgcgatcgtc gtcacgttca cgctcctcgg catcctgatg gcggccctcg tgggtgccgc
     2521 cggagcccag acgatcgcgg ccaacccctg ggtgaacctg ttcatcgccc tcgtgctgat
     2581 cggattcgcc ctgtcgctcc tggggctgta cgagctccgt gtgccgaacg ccctgctcaa
     2641 cttcacgaac cggcagtcga cgcagcgggg cggagcggtc ggagtcctgt tcatggggct
     2701 cacgctcacc ctcgtgtcgt tctcgtgcac ggcgcccttc gttggggggc tgctggccgc
     2761 tgcggccggg ggaacgtggg cctggcccat gctgggcatg gtcgtgtact cggccgcatt
     2821 cgccctgccc ttcgtgctgc tcgccctgtt cccgcgcgcg ctctcgaccc tgccgaaatc
     2881 cggagcctgg atgaacgtcc tcaaggtcgt gctcggcttc gtggagctgg cggcctcgct
     2941 caagttcctc tccaacgccg acctggtctg gggttggggc atcctctccc ggccgctggc
     3001 catcgcgatc accgtcgtga tcctgttcgt cgccggggtc tacctcctgg ggaagctgcg
     3061 cctttcgcac gaggagccgg cgccggccgt gggtgtgggg cggctgcttg ccggggtcgg
     3121 gttcatcgcc gtgagcctgt acatgctccc ggggctcttc ggagcgcccc tcaattcgct
     3181 cgacgcgttc ctgccgccgc ggcagggaac cgacgtcagt cttctcgccg tcctggggcc
     3241 gggttccgtt ccgcgtgacg acgaagagtg gatcgtcgac gacatcgacg ccgccgtgga
     3301 cttctcgggc gtatcgaatc ggccgctctt cgtcgacttc accgggtaca cgtgcacgaa
     3361 ctgccgcgag atggaggcga atgtcttccc ggcggccccg gtgcgcgagc ggctcggccg
     3421 cgacttcgtg ctgcttcggc tctataccga cggactcgag cgcggaacgg agttccagcg
     3481 ctaccaactg cagctgacgg gcacggtcgc gcttcccacg tacgccgtgc tcgcggccga
     3541 cgggaggaca ctccttgcgg tggagagcgg catcatgagc cgcgacgcgt tcgtccgctt
     3601 cctggacggg gcctcgtcgg ccttctcgcg tctatagggc ctgccgcttc agtcgccgcg
     3661 cggcccggaa accggaacga ctagcacggg ccgatgcgcc tggttgacga cggcctggga
     3721 cacgctgccg agcaggaagc gttcgagcat gccgtgatgc cgctttccga cgacgatcag
     3781 gtcggcgtcg agccgctcgg cctcctggag aatggtgcgg gcgggatctc cctggagcag
     3841 ggcaggccgg acgacccgtc tgaagcgttc ccggacgaag gcgtcgaact catgctgctc
     3901 ggcctgctcc atggacgctt ggaccgtggg gtcgtaggcg gcgtaggcca tgtcgccgaa
     3961 gccggtcatc gggtcgacgg gcatgggaga ggccggcgac ggctggtaca cgtggagcac
     4021 gcacaggtcg gccccctgtc gaagggcgat ctcctccacg gacgacgtga cgtcggcggg
     4081 ttcgctcagg tcgacggcaa aaagaatcgt catggctccc tccgctgcat accggatgat
     4141 aggcccgtcg aaaggggagc gcaaacggcg gttgcggagg ttatgcggca cggcgtgagc
     4201 gggtcatcgg ggagaatccg aagcccccga tgccacgcag caccccgtcc cgtctccact
     4261 ggcccggccc ggccacgggc gagcccgtgc ccgccgtgct gctgcagcgg gcgcagcacg
     4321 gggaccggga ggcgtgccat cggctcctgg gctgggcata ccggtcggcg tgtgactact
     4381 acgtgggcaa ggtcggcgtg gaagtcctcc tttcgccgac cgacgccgaa gagctggccg
     4441 ggatgttcct gctcgagttc gagcgcgcgt ggccgcggat tcggagtctg gggcactaca
     4501 cgcggcggat gctccgcaac aatctccgcc gctacctgcg gaggaagcgt gaactcaggc
     4561 gtcgcgaggc gccttacgcc tcgcacgaga tggaccggat tccggatgcc gcgctccacg
     4621 tttccgaatc ccatcccatc gagcgatggg gagacgacga ggccgcccag ttcgccgcgg
     4681 tccgggagac cctttcgcgc acggaccccc tcacccggga cctcatggca ctgcgaagcg
     4741 gtgatccgcc gctttcctat gccgtcatcg ccgtccggct gaacacgacc gagacggcgc
     4801 tgcgcatgcg tgcggcccga ttctaccagg ccgtacggga cacccacagg gccatggccg
     4861 gtcccggctg atgaggccgg tcacggacga ttcgaggagg aatgccatgc cgatcgattc
     4921 cggatacgaa gacggcaccg aacccctgag cgtcgagtgc atccatcgat gggtccgtct
     4981 ggctgccgtt acgcgggcct ccgaagacgc gggctccgcc ggagccaggc tggcgatggt
     5041 ggccgcctgc atcgtgtgcc tgaccctggg ggcgaccgcc cgtgagatca cccattgggc
     5101 gctcggtgat acctcgttcg gcccgttctc ggcacgccag accccgacgg atccctggtt
     5161 ccggtgcggc ggcggattcg cgccggggcg agcggaggaa gcgtggcgcg aaccgagcgg
     5221 atgggcgccc tcggacgacg ccttcgccct tccgataccc ggattccgga cgggcccggt
     5281 gaaccaggaa ggagggacgc cgtagaccgg gcgaaatccg aacgccccgg ccacggtccg
     5341 ccccccatgc cccactttct cgatacgccc gttaccctcc tcctgctcgt cgtcaatgtg
     5401 ctggtcagcg gctacgcgct gtacggcgat gaggggcttc tggaccggct gtcctttcga
     5461 cccaggcaga tcatccggga tcgggagtat taccgcctgg tgacggcggg attcgtacac
     5521 ggtgggttcg ggcacctggc cgtgaacatg ctcac
//
LOCUS       DBNHQZ010000359         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3221071, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000359 DBNHQZ010000000
VERSION     DBNHQZ010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3221071"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="AC16YU_09710"
     CDS             <1..1274
                     /locus_tag="AC16YU_09710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065543.1"
                     /translation="VSPMSLFRIASMSKSFTALAILQLRDAGKLGLDDPASEYIPEME
                     QLAYPTSDSPGITIRHLLTHGAGFPEDNPWGDRQLADSDEELLQLVADGISFSNAPGV
                     AYEYSNLGFALLGQIVQRVSGMEFQAYTREHIFGPLGMDATVWEYTDAPPDRLAPGYD
                     WIDDAYVRIPLEHHGSFGAMGGLITSIEDFTRYVALHLSAWPPRDGEDSGPLKRSSLR
                     EMHHPWRFASLGARSGGRDCASVRAYAYGLSWSRDCDGKVTVGHSGGLPGFGSNWTIL
                     PDYGLGVMSFDNRTYGGTSSINAAVLDSIVAMAGLGPRTLPASDILETRKNEIVAILP
                     GWDGAEDSGLFAENFFLDHRLTDVVQRTKELFAEAGEITGVGPLEPENQLRGTFRIEG
                     RMKDLEVFFTLTPETVPLVQQLTIRAVERGR"
     gene            1406..>1938
                     /locus_tag="AC16YU_09715"
     CDS             1406..>1938
                     /locus_tag="AC16YU_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR6065544.1"
                     /translation="MAAAILLVVPVASAQTLPFPLAIPLEGVESFDASVPTPEQVIGH
                     RIGTRHTRPDQVVDYARAVDEASDRVTLGEHGRTWEGRPLIHALITAPTNQGRLEEIR
                     RQNLRLTHDPASVSDAEIEAMPVVLYQGFSIHGNEASGTDAAVLYLYWLAAGRGPAVD
                     AALANAVVVWDPMLNPDG"
BASE COUNT          314 a          663 c          651 g          310 t
ORIGIN      
        1 ccgtgagccc gatgtcgctc ttccgcatcg cctcgatgag caagagcttc acggcgctgg
       61 ccatcctcca gctgcgcgat gccgggaaac tgggcctgga cgaccccgcg tcggaatata
      121 tcccggagat ggagcagctc gcctatccca cgtccgactc ccccggaatc actatccgcc
      181 acctgctcac ccacggggcc gggttcccgg aggacaaccc gtggggtgac cggcagctgg
      241 cggactcgga cgaggagctg cttcagctcg tcgcggacgg catctcgttc tccaatgcgc
      301 cgggcgtggc gtacgaatac agcaacctcg gtttcgcgct cctcggacag atcgtccagc
      361 gggtctcggg aatggagttc caggcataca cccgggaaca cattttcggg cccctcggca
      421 tggacgctac ggtgtgggag tacacggacg caccgccgga tcgcctggcg cccggctacg
      481 actggatcga cgacgcgtac gtccgcatac ccctggagca ccatggatcg ttcggcgcga
      541 tgggcgggct catcacgtcc atcgaggact ttacgaggta cgtggccctg cacctgtccg
      601 cctggcctcc ccgcgacggc gaggattcgg gaccgctgaa gcggagctcg ctgcgcgaga
      661 tgcaccatcc ctggcggttc gcctcgctcg gcgcccgctc cggcggcagg gattgcgcct
      721 cggtccgggc ctacgcctat ggtctgagct ggagccggga ctgcgacggc aaggtgaccg
      781 tcggccacag cggcgggctg cccggattcg gaagcaactg gaccattctt ccggactacg
      841 gcctcggcgt gatgtccttc gacaaccgga cgtacggggg cacgagctcc atcaacgccg
      901 cggtcctcga ctcgatcgtc gctatggccg gcctcgggcc gcgcacgctt cccgcctccg
      961 acatcctcga gacgcggaag aacgaaatcg tggcgatcct gcccggctgg gacggcgccg
     1021 aggactcggg gctgttcgcc gagaacttct tcctggacca tcgtctgacc gatgtcgtgc
     1081 agcggacgaa ggagctcttc gcggaggcgg gggagatcac cggcgtcggg ccgctggagc
     1141 ccgagaacca gctccgggga acgttccgga tcgagggtcg gatgaaagac ctcgaagtgt
     1201 tcttcaccct gaccccggag acggtgcccc tggttcagca gctgacgata agggcggtgg
     1261 agagaggtcg gtgaccgcag ggtattcccc ggttgcgcat tcgctcttga tggggtattc
     1321 agcgaccggt ccctgaacga acgtccttcc cgttcccatg atcgctcgaa gccctcgcag
     1381 cccgtcagcg ccgactgtcg caacgttggc ggccgcaatc ctcctggtcg tacccgtggc
     1441 cagcgcccag accctcccct tcccgctcgc gattccgctg gagggcgtcg agtcgttcga
     1501 cgcctcggtg ccgacgcccg agcaggtgat cggccaccgg atcgggaccc ggcacacgag
     1561 gccggaccag gtggtggact acgcccgggc cgtcgacgag gcgagcgatc gcgtcacgct
     1621 gggcgagcac gggcgcacat gggaagggcg gcctctgatc cacgccctca tcacggcgcc
     1681 gaccaaccag ggccggctcg aggagatcag gaggcagaac ctgaggctga cgcacgaccc
     1741 ggcctcggta tcggacgccg agatcgaagc catgcccgtc gtcctgtacc aggggttctc
     1801 gatccacggc aacgaggcct cggggaccga cgcggcggtg ctgtacctgt actggctcgc
     1861 cgcggggcgg ggaccggcgg tcgacgccgc gctcgcgaat gccgtcgtcg tctgggaccc
     1921 gatgctgaat cccgacgg
//
LOCUS       DBNHQZ010000360         6234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2544779, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000360 DBNHQZ010000000
VERSION     DBNHQZ010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2544779"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="AC16YU_09720"
     CDS             <1..666
                     /locus_tag="AC16YU_09720"
                     /inference="COORDINATES: protein motif:HMM:NF018129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HIR6065545.1"
                     /translation="VSDVPSGLYGSRKESAGGDGADWTRSRLRRHARVLGQIGHRITV
                     LPLSFGTVFASEAEVKEMLTLKAREYAATLERLNGRVEWTVRMVRDDYRMLERMVDLL
                     DADGMDLSSLPDDVAGELDAALAECEDPGKMMSVVTSQCVAHARAHLELYADSSVDRR
                     SGSDAREEASGLNRAYLVPRDGATAFRKALESLAVQWSGLGLGFEVSGPWPPYHFAEG
                     SFE"
     gene            734..1978
                     /locus_tag="AC16YU_09725"
     CDS             734..1978
                     /locus_tag="AC16YU_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065546.1"
                     /translation="MASVPFQTERFLARRYLAGAEGRPEGRRFLRFVTYVAVGGVAVG
                     VAALLLAFSIVRGFSREIENKIIGFGAHVQVESLRDAPLGDADALALRLAGRDGVDAV
                     SPVVQEFVLLRRSSARIEGISIWGTDRVPDFVGRSLVAGTGDLSDDSDGARRIVVGAR
                     LARQLDVAPGDRVTAFSVGGGQATPGRVRVKPFVVAGVFESDLADFDDVFAFVAIDHA
                     RDLLSYGPEQVTRFDLTLRDPSRADAVAAAVGEDEGFPVMARSIYEVYSSLFAWVRLQ
                     QQIIPMVIAVIILVAAFNIIGTLLMMMLEKTREIGILMAMGATPTLLRRTFLLLGALI
                     GVTGIVIGEGLALALALLQQRYGIIPLPAEAYFMKTAPIELAALDFALVAVLTVLLCA
                     LAAYLPARFAGRIQPIRAIHFR"
     gene            2025..2279
                     /locus_tag="AC16YU_09730"
     CDS             2025..2279
                     /locus_tag="AC16YU_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6065547.1"
                     /translation="MPRPTVTIRLFSVLRERAGSDRLELAADGFRTVGDAAEALYERF
                     PALAPYRPVVRLARNQVYADASEPLQAGDEIALITPTSGG"
     gene            2284..2730
                     /locus_tag="AC16YU_09735"
     CDS             2284..2730
                     /locus_tag="AC16YU_09735"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR6065548.1"
                     /translation="MEEAVDSILLGADPLDLAAAWEVSADDRAGAVDLFVGTTRRFTA
                     GRETVRLEYEAYGSMALSEMRRIVTDARSRWEILRCVVHHRIGTVAVGEPSVIIAVST
                     RHRAEAFEACRFLIDTLKREVPIWKREWFTDGTSEWVGGEAPAGPA"
     gene            complement(2735..3706)
                     /locus_tag="AC16YU_09740"
     CDS             complement(2735..3706)
                     /locus_tag="AC16YU_09740"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6065549.1"
                     /translation="MIDRSVIEAQLPATIRETRFESLGARYGGKVRDTYVVDDRLVLV
                     ATDRISAFDHVLRQTIPFKGQVLNRLAAYFFAETGDVMPNHILSVPDPNVTVAVRCEP
                     LPIEFVVRGYLAGHAWRTYRGGGRVLCGQPLPEGLVQNDRLPRPILTPSTKAQEGHDE
                     DITHAEILERGILSAAELEELEALALALFARGTDIAARRGLILVDTKYEFGRTPDGRF
                     VVIDEIHTPDSSRYFYAEGYEDLLAQGLPQRQLSKEFVREWLMEHGFRGLEGQALPDL
                     PDAFRVEVSARYIELFEQVTGLAFKPDTRPDPVDRIRENLDSVGIPV"
     gene            complement(3717..4757)
                     /locus_tag="AC16YU_09745"
     CDS             complement(3717..4757)
                     /locus_tag="AC16YU_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6065550.1"
                     /translation="MPTGHSKVTIYDVAARAGVAISTVSRVLNDSPDVSDRTRARVMK
                     AVEELRYRPDRTAKKLAQQRTLTLAIALPTFITPFHNELLKGDRMCLLDMEIDLLLCD
                     RGWRAPFRTLQNFLRRGTVDGLLLAGVSMDERVASELLSVRAPVVLIGSAWPGFDSFE
                     WDDTAGVLDGVNHLVGQGHRRIGMIRSFTDSEIQLNRVAGYRSALEAAGIPFDERLVV
                     SGDTTKHAGFSEEAGYEAMQRLLAIEEEPVTAVFASSDVQALGAWAAIIDSGRRVPDH
                     VAIVGYDDIKSSQFIGLSSVDQSIQQIGHSATERLLARLAGELAEAPTARRITPKLRV
                     RRSSVHRINQES"
     gene            4917..>6234
                     /locus_tag="AC16YU_09750"
     CDS             4917..>6234
                     /locus_tag="AC16YU_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6065551.1"
                     /translation="MPDQTQDKELLEIALPIEGMTCAACATRIERRLSRTDGVRRAAV
                     SYATEEALVGVEAEGQDFGRLVQAVEQAGYRVRTVSAEARVRPGAERAAGDVVRDTQR
                     VPGVLRVETAEDGDGVVVRATYVRGATDLDVLARALGAEPDRPVGTASGEADALEAEH
                     LKRYRDLRKRFVVAVVLSLPVVVVSMAHDVLDFPGSRWLLWALSTPVVAWSGAEFFVL
                     AWRAARHRTTDMNTLVAVGVGTAYVASAAAVLAPSFFEATGGHADVYFEAAAVIVTLI
                     LLGRLLEERAKGRTGSAIRALMKLQPEEARVRRGGVETVVPVRDVRIGDEVVIRPGER
                     VPVDGEILTGGSAVDESMVTGEPIPAEKGPGDPVVAGTVNTTGALVCLVTRVGRDTLL
                     RQIVEMVRHAQADKPPIQRLADRIAAVFVPAVIGIALAAAATWWFLG"
BASE COUNT          900 a         2069 c         2275 g          990 t
ORIGIN      
        1 gtcagtgacg tgccgagcgg cctgtacggc tcccggaagg aatcggcggg gggtgacggt
       61 gcggactgga cgcgctcccg gctgcggcgg cacgcccgcg tcctgggaca gatcggccat
      121 cgcatcaccg tcctgcctct ttcgttcgga acggtgttcg cgtccgaggc cgaggtcaag
      181 gagatgctca cgctgaaggc gagggagtac gccgccacgc tcgaacggct gaacggccgc
      241 gtggaatgga ccgtgcgcat ggtgcgggac gactaccgca tgctcgagcg catggtggac
      301 ctcctggatg ccgacgggat ggatctttcg tcgctgcccg acgacgtggc gggcgagctg
      361 gacgccgcgc tcgcagagtg cgaggatccg ggaaagatga tgtctgtggt cacgtcgcag
      421 tgcgtggccc acgcgcgggc ccacctggag ctctacgcgg acagctcggt cgaccgccgc
      481 tcgggctcgg atgcccgcga ggaggcctcc gggctgaacc gcgcctatct cgtaccgcgt
      541 gacggggcca cggcgttccg caaggcgctc gagtcgctcg ccgtgcagtg gtccggactc
      601 gggctgggct tcgaggtgag cggcccctgg ccgccgtacc acttcgccga aggctcgttc
      661 gagtagggga tacgccccgg caggcgggat caaagcgcgg gccgtgacgt gagcggggca
      721 ccgtccctcc gccatggcat ccgtcccgtt ccagaccgag cgcttcctgg cccgacgcta
      781 cctggcgggt gccgaggggc gtcccgaggg tcgccggttc ctccggttcg tgacctacgt
      841 cgcggtcggg ggcgtggcag tcggggtggc ggcgctgctc ctcgcgtttt cgatcgttcg
      901 gggcttcagc cgggagatcg aaaacaagat catcgggttc ggcgcccacg tgcaggtcga
      961 gagtctgcgc gatgcgcccc tgggagacgc cgatgcgctc gcacttcgcc ttgccggccg
     1021 cgacggcgtg gacgcggtgt cgcccgtggt gcaggaattc gtgctgctcc ggcgctcgtc
     1081 ggcccggatc gagggcatct cgatctgggg cacggaccgc gtgccggact ttgtcggacg
     1141 gagcctggtg gccggcacgg gcgacctgtc ggacgattcc gacggagcgc ggcgaatcgt
     1201 cgtcggcgcg cgcctggccc ggcagctgga cgtcgccccg ggcgaccggg tgaccgcctt
     1261 ctcggtagga ggtggccagg cgaccccagg ccgcgtgcgc gtcaagccct tcgtcgtggc
     1321 gggcgtgttc gagtccgacc tggccgactt cgacgatgta ttcgccttcg tggcgatcga
     1381 tcacgcccgt gacctgctct cgtacgggcc ggaacaggtc acccggttcg atctcacgct
     1441 gcgcgacccc tcgcgggccg acgccgtcgc agcggccgtc ggcgaggacg agggattccc
     1501 cgtgatggcc cggtccatct acgaggtcta ctccagcctc ttcgcctggg tgcggctgca
     1561 gcaacagatc atcccgatgg tgatcgcggt catcatcctg gtcgccgcct tcaacatcat
     1621 cggaacgctg ctcatgatga tgctcgagaa gacccgcgag atcggaatcc ttatggccat
     1681 gggagccacg ccgacgctgc tgcggcggac cttcctgctc ctcggcgcgc tgatcggggt
     1741 gacgggcatc gtgatcgggg aggggctcgc cctggccctg gccctgctgc agcagcggta
     1801 cggaatcatt cccctgcccg ccgaggccta cttcatgaag acggcgccga tcgagctggc
     1861 ggcgctcgac ttcgcgctcg tcgcggtgct gaccgtgctt ctctgtgccc tggccgcgta
     1921 tcttccggcc cggttcgccg ggcggatcca gcccatccgc gccatccact tccggtgatc
     1981 cggacgcccg ccgaccgtcc ctgccgtgag cgccgccccg atccatgccc cggcccaccg
     2041 tcacgatccg actgttcagt gtgctgcgcg agcgcgccgg aagcgaccgc ctggagctcg
     2101 ccgccgacgg cttccggacc gtaggcgacg ccgccgaggc gctatatgaa cggtttccgg
     2161 cgctggcgcc ctaccgaccg gtggtgcgcc tcgcgcgcaa ccaggtctat gcggatgcgt
     2221 ccgagcctct tcaggcgggc gacgagatcg ccctcatcac gccgacgagc gggggctgac
     2281 gccatggagg aggccgtgga ttcgatcctg ctcggcgccg atccgctgga tctcgccgcc
     2341 gcctgggagg tgtccgcgga cgaccgcgcc ggggcagtgg atctcttcgt cggcacgacg
     2401 cgccgcttca cggcgggacg cgagaccgtt cggctcgaat acgaggccta cggatcgatg
     2461 gcgctctccg agatgcggcg catcgtgacg gatgcgcgct cgcgctggga gatcctccgc
     2521 tgcgtggtcc accaccggat cggcacggtg gccgtcggcg agccgagcgt gatcatcgcc
     2581 gtctcgacca ggcatcgcgc cgaggcattc gaggcctgcc gcttcctgat cgacacgctc
     2641 aagcgcgagg tcccgatctg gaagcgggaa tggttcaccg acggcacctc cgagtgggtc
     2701 ggaggggaag cgccggcggg cccggcctga tggttcagac cggaatccct accgagtcca
     2761 ggttttcccg gatccggtcg acgggatcgg gccgggtgtc gggcttgaac gcgaggccgg
     2821 tgacctgctc gaagagttcg atgtagcggg ccgaaacctc cacccggaag gcatcgggga
     2881 gatcgggaag cgcctggcct tcgaggccgc ggaatccgtg ctccatgagc cattcgcgca
     2941 cgaattcctt cgaaagctga cgctgcggaa gaccttgcgc cagaaggtct tcgtaccctt
     3001 cggcatagaa gtagcgcgaa gagtcgggcg tatgtatctc gtcgatcacc acgaaccgcc
     3061 cgtccggcgt ccggccgaac tcgtacttgg tgtccacgag gatcagaccg cggcgggccg
     3121 cgatgtccgt gcctcgggcg aagagagcga gggccagggc ctcgagttcc tcgagctccg
     3181 cggccgaaag gataccgcgc tccaggatct ccgcgtgggt gatgtcctcg tcgtgccctt
     3241 cctgggcctt ggtcgagggc gtcaggatgg gcctcgggag gcggtcgttc tgaacgagcc
     3301 cctcggggag cggctgcccg cagagcacgc ggccgccgcc ccggtacgtg cgccaggcat
     3361 gcccggccag gtagccgcgc accacgaact cgatgggcag cggctcgcac cggacggcga
     3421 cggtgacgtt cggatccggt acggaaagga tgtgattggg catcacgtcc ccggtttcgg
     3481 cgaagaagta ggccgcgagg cgattcagga cctgtccctt gaagggaatg gtctgacgca
     3541 ggacgtggtc gaaggccgag atgcggtcgg tcgccacgag cacgagccgg tcgtcgacca
     3601 cgtaggtgtc gcggaccttc ccgccgtagc gggctccgag gctctcgaag cgggtctcac
     3661 ggatggtggc gggaagctgg gcctcgatga cggagcgatc gatcatgggg cggaaatcag
     3721 gattcctggt tgatgcggtg cacgctggat cggcgcacgc ggagtttcgg tgtgatgcgg
     3781 cgggccgtgg gcgcttcggc gagctcgccg gcgaggcgtg cgagcagccg ctcggtggcc
     3841 gagtgcccga tctgctggat gctctggtcc acgctcgaga gtccgatgaa ctggctcgac
     3901 ttgatgtcgt cgtaccccac gatggccacg tggtccggca cacgcctccc ggagtcgatg
     3961 atcgcggccc aggcgccgag cgcctgcacg tcgctcgagg cgaagaccgc cgtgaccggc
     4021 tcctcttcga ttgcgagcag gcgctgcatc gcctcgtagc ccgcctcctc gctgaatccc
     4081 gcgtgcttcg tggtgtcgcc ggagaccacg aggcgctcgt cgaacgggat gccggccgcc
     4141 tcgagcgccg agcggtaccc cgcgacccgg ttcagctgga tctcggaatc ggtgaaggag
     4201 cggatcatgc cgatccggcg gtgcccctgg ccgaccaggt ggttcacgcc gtcgagaacc
     4261 cccgcggtat cgtcccactc gaaggaatcg aagcccggcc aggccgagcc gatgagcacg
     4321 acgggagccc ggacggagag cagctccgac gccacgcgct cgtccatgct gacaccggcc
     4381 agcaggagtc cgtcgaccgt gccgcgccgc aggaagttct ggagcgtgcg gaagggcgcc
     4441 cgccagcccc ggtcgcagag caacaggtcg atctccatgt ccagcaggca catcctgtcg
     4501 cctttcaaaa gctcgttgtg aaacggcgtg atgaaggtgg ggagcgcgat cgcgagcgtc
     4561 agcgtccgct gctgggccag cttcttggcc gtgcggtccg ggcggtaccg aagctcctcg
     4621 acggccttca tgacccgcgc ccgtgtccgg tccgacacgt cgggcgagtc gttgagcacg
     4681 cgcgagaccg tggaaatggc cacgcctgcc cgggcggcga cgtcgtagat agtaaccttg
     4741 gaatggccgg tcggcatgcg ggctccctgc gccggaaagg acgcgcgaaa ggtagcgatc
     4801 gggcgtcata cgcgcgaacg ggaacccggg atcggtacgg ttcggcgtga cccgccgcgc
     4861 gcggggagga aaccgcgggc cggatgccgc gtgcaggaat cccttccgcg ttcgccatgc
     4921 ccgaccagac ccaggataag gagcttctcg agatcgccct tccgatcgag ggcatgacgt
     4981 gcgcggcctg cgcgacgcgc atcgagcgtc gactctcccg gaccgacggg gtgcgacggg
     5041 ccgcggtaag ctacgcgacc gaggaggcgc tcgtcggcgt ggaggcggag ggacaggatt
     5101 tcggtcggct ggtccaggcg gtggagcagg ccggctaccg ggtccgcacg gtctcggccg
     5161 aggcccgggt ccgtccgggc gcggaacgtg cggccggcga cgtggtgcgc gacacgcagc
     5221 gcgttccggg cgtcctgcgc gtcgagaccg cagaggacgg cgacggggtc gtcgtgcggg
     5281 ccacctacgt gcgtggcgcc acggacctgg acgtcctggc gcgggcgctc ggcgccgagc
     5341 ccgaccggcc ggtcggcacg gcatcgggcg aggccgatgc cctcgaggcg gagcatctga
     5401 agcggtacag ggacctgagg aagcgattcg tcgtggccgt cgtcctgagt cttccggtcg
     5461 tggtcgtgag catggcgcac gatgtgctcg attttccggg ctcgcgctgg ctgctgtggg
     5521 cgctttcgac gccggtcgtc gcatggtcgg gtgccgagtt cttcgtcctg gcgtggcggg
     5581 ccgcccgtca ccggacgacc gacatgaaca cgctcgtcgc ggtgggagta gggacggcct
     5641 acgtcgcgag cgccgccgcc gtcctcgcgc cgtcgttctt cgaggcgacc ggcggccacg
     5701 ccgacgtcta tttcgaggcg gcggccgtca tcgtgacgct gatcctgctc ggccgcctgc
     5761 tcgaggagcg cgcgaagggc cggacgggca gtgcgatccg cgcgctcatg aagctgcagc
     5821 ccgaggaggc gcgggtccga cgcgggggcg tcgagacggt cgtgcccgtg cgcgacgtcc
     5881 gcatcgggga cgaggtcgtg atccggccgg gcgagcgcgt tccggtcgac ggggagatcc
     5941 tgaccggagg ttcggccgtg gacgagagca tggtcacggg cgagccgatt ccggcggaaa
     6001 agggccccgg agacccggtc gtagccggaa cggtgaatac caccggcgcc ctggtctgcc
     6061 tggtcacgcg cgtcggccgc gacaccctgc tcaggcagat cgtcgagatg gtccggcacg
     6121 cccaggccga caagcccccg atccagaggc tcgccgaccg cattgcggcc gtcttcgttc
     6181 cggccgtgat cgggattgcc ctcgcagcgg ccgccacgtg gtggttcctg ggcc
//
LOCUS       DBNHQZ010000361         4242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1058891, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000361 DBNHQZ010000000
VERSION     DBNHQZ010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1058891"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..520)
                     /gene="rpsK"
                     /locus_tag="AC16YU_09755"
     CDS             complement(122..520)
                     /gene="rpsK"
                     /locus_tag="AC16YU_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR6065552.1"
                     /translation="MAKRQRTSGRTRKKNVVVESNGQVHIKATFNNVLVTVTDQYGNT
                     ISWASSGKMGFKGSRKNTPYAAQVASSEAAKEAYDLGLRRVEVYVKGPGSGRESAIRA
                     LSAVGLEIATIRDVTPMPHHGCRPPKRRRV"
     gene            complement(568..948)
                     /gene="rpsM"
                     /locus_tag="AC16YU_09760"
     CDS             complement(568..948)
                     /gene="rpsM"
                     /locus_tag="AC16YU_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR6065553.1"
                     /translation="MPRISGVDVPPTKRGEIALTSIFGIGRARAIEILLKAGIDPDHK
                     PESWTDEQTRTVRRMIEDDYQVEGQLRTDTQLNIKRLMDIGCYRGLRHRRGLPVRGQR
                     TQTNARTRKGKRKTVAGKKQAPRK"
     gene            complement(985..1101)
                     /gene="rpmJ"
                     /locus_tag="AC16YU_09765"
     CDS             complement(985..1101)
                     /gene="rpmJ"
                     /locus_tag="AC16YU_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR6065554.1"
                     /translation="MKVRASVKKRSPEDKIVRRKGRVYIINKKNPKHKQRQG"
     gene            complement(1222..2049)
                     /gene="map"
                     /locus_tag="AC16YU_09770"
     CDS             complement(1222..2049)
                     /gene="map"
                     /locus_tag="AC16YU_09770"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6065555.1"
                     /translation="MIHLKNEREIAGLRAAADLVGRTLGEVARYVVPGATTRALDEVA
                     EAFIRDHGAVPAFKGYGPSTNPFPSTLCASVNDVVVHGIPDDRPLEEGDLLSVDCGVV
                     LDGFVGDSAYTFAVGEADPDRLRLCRTTYDALMLGVAAARVGSRIGDIGHAIQARCES
                     AGYGVVRDLVGHGIGRSLHEEPSVPNFGRRGSGRRLKDGLTVCIEPMVNAGTSEVTTD
                     PDGWTVRSADRSASAHYEHMVAVRRSGTEVLTTFRYVEDVLGVVPYAPIASEVEADG"
     gene            complement(2068..3384)
                     /gene="secY"
                     /locus_tag="AC16YU_09775"
     CDS             complement(2068..3384)
                     /gene="secY"
                     /locus_tag="AC16YU_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6065556.1"
                     /translation="MAGFTESLRNIWKIEELRKRITYTVGLLIVYRLGAFVTLPGVDA
                     SVLADINQTADPNNLFGFLDLFVGGAFSAAGIFALGIMPYITASIIIQLMGAVVPYFQ
                     KLQREGEEGRRKITQLTRYGTIAITALQSVGFAIQLQYGATGQAIVINPTFFMVTTVI
                     VLTAGTMFVMWLGERITESGIGNGISLIIMIGIIAYLPSSMYNELQLTGNLFIFLLEI
                     GVFALVTAGVVLVTQGMRRIPVQYAKRVVGRKVYGGTTQYLPLRVNAAGVMPIIFAQS
                     IMFVPATVASFFPDSEFMQRFGVFFTDIGSFGYSFVFFIICVFFTYFYTAIAVNPREM
                     ADTMKRQGGFIPGIRPGKQTSEFIDNILTRITLPGSVFLGLVAILPAFAIQAGVTMGF
                     AQFFGGTSLLILVGVTLDTLQQVESHLLMRHYDGFMKTGKIRGRRF"
     gene            complement(3408..3869)
                     /gene="rplO"
                     /locus_tag="AC16YU_09780"
     CDS             complement(3408..3869)
                     /gene="rplO"
                     /locus_tag="AC16YU_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR6065557.1"
                     /translation="MDLSNLKPAEGSTHNEKRIGRGQGSGYGGHSSTKGNKGQKSRSG
                     AKFPVWFEGGQMPLQRRVPKFGFKNRFRVEYRPVNVGRLQQLVDAGALDAAAPVTPDV
                     LRAAGVIGKADLVKILGSGDITASLQVTAHAFSASARDKIEKAGGSSTVAD"
     gene            complement(3928..4110)
                     /gene="rpmD"
                     /locus_tag="AC16YU_09785"
     CDS             complement(3928..4110)
                     /gene="rpmD"
                     /locus_tag="AC16YU_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR6065558.1"
                     /translation="MAQLKITQVRSTIRRPATQKRTLEALGLRRMNQTVIHEESPQLR
                     GMLDKVNHLVRVEPAE"
BASE COUNT          668 a         1380 c         1425 g          769 t
ORIGIN      
        1 ctctgtctcg ggccagttcg gttctttctc gtttgccggt tcaacaaacg tacgggaacg
       61 caccgtgcgc cccgtagccg gactcatgcc tggatcgcgc ggggcgatcc gcgacgccgc
      121 gtcagacgcg acgacgcttc ggcggccggc agccgtggtg aggcattgga gtgacgtccc
      181 ggatggttgc tatttcgaga ccgacggccg acagcgcgcg gattgccgac tcccggccgg
      241 agccgggacc cttcacgtac acctcgaccc ggcggagacc caggtcgtag gcctccttgg
      301 ccgcctcgct cgacgcgacc tgggccgcgt agggcgtgtt cttgcggctt cccttgaagc
      361 ccatcttgcc cgagctggcc caggagatgg tgttgccgta ctggtcggtg accgtgacga
      421 gcacgttgtt gaaggtcgcc ttgatgtgaa cctggccgtt cgattcgacg accacgttct
      481 tcttgcgggt acgaccggag gtacgctgtc ttttcgccat gtctctggag aggatgaaag
      541 ggcggccctg gcggacgccc cgtcacatta cttgcggggg gcctgcttct ttccggcgac
      601 cgtcttgcgc ttgcccttgc gggtccgcgc attcgtctgc gtccgctggc cgcggaccgg
      661 caggcctcga cggtgacgaa gtccccggta gcagccgatg tccatcagcc gcttgatgtt
      721 gagctgcgta tcggtccgca gctgaccctc gacctggtag tcgtcctcga tcatgcgacg
      781 aacggtccgt gtctgctcgt cggtccagga ctccggcttg tggtcggggt cgatgcctgc
      841 cttcagcagg atctcgatcg cgcgcgcgcg gccgatcccg aagatggacg tgagcgcgat
      901 ctcgccgcgc tttgtcgggg gtacgtctac gcctgaaata cgtggcatat cgcttctcga
      961 gccgtggtgt ggtcccggcg gggatcagcc ctggcgctgc ttgtgcttcg gattcttctt
     1021 gttgatgatg tacacgcgtc cctttcggcg gacgatcttg tcctcgggac tgcgcttctt
     1081 cacgctggca cggaccttca tggcatcagc gcggccagcc tggccgcatt tggtgcagaa
     1141 cagatagaac gcatcgggtt ccggttggtt cggatccgcc ggccgccggc cggcgagagg
     1201 atccgtgccg ggttcagggg gtcaaccatc ggcttccacc tccgaggcta tcggggcgta
     1261 cgggacgacg cccagcacgt cctcgacgta gcggaacgtg gtgaggacct ccgttccact
     1321 ccgccgcacg gccaccatgt gctcgtagtg cgccgaggcc gagcgatcgg ccgagcggac
     1381 ggtccagccg tccgggtccg tggtgacttc gctcgttccg gcattcacca tgggctcgat
     1441 gcagaccgta aggccgtcct tcagccggcg gccgctgccg cggcgcccga agttggggac
     1501 ggacggctcc tcgtgaaggc tccgcccgat gccgtggccg accaggtcgc ggaccacgcc
     1561 gtacccggcc gactcgcaac gcgcctggat ggcatgcccg atgtcgccga tccggctgcc
     1621 gacccgggcc gccgccactc cgagcatcag ggcatcgtac gtcgtgcggc acagccgcaa
     1681 ccggtccgga tccgcctccc cgaccgcgaa cgtgtaggcg ctgtcgccca cgaagccgtc
     1741 gagcaccacg ccgcagtcca ccgagagcag gtcgccctcc tcgagcgggc ggtcgtccgg
     1801 aatgccgtgc accacgacgt cgttcaccga cgcgcagagc gtggacggga aggggttcgt
     1861 cgaagggccg tatcccttga aggccggcac cgcgccgtgg tcccggatga acgcctccgc
     1921 cacctcgtcc agggcacgcg tcgtcgcgcc cggcacgacg taccgcgcga cctcgcccag
     1981 cgtgcgcccc accaggtcgg cggcggcacg cagccctgcg atttcccttt cgttcttgag
     2041 gtggatcatg gatgggtgcg gacgggccta gaaccggcga ccgcggatct tcccggtctt
     2101 catgaacccg tcgtagtggc gcatcagcag gtggctctcg acctgctgca gggtgtcgag
     2161 cgtgacaccg acgagaatga gcaggctcgt accgccgaag aactgcgcga agcccatcgt
     2221 gacgcccgcc tggatcgcga acgcgggcag gatcgcgaca aggcccagga agacggagcc
     2281 gggaagcgtg atccgggtca ggatgttgtc gatgaactcg ctcgtctgct tgccggggcg
     2341 gataccggga atgaacccgc cctgccgctt catcgtgtcg gccatctccc tcgggttcac
     2401 ggcgatggcc gtgtagaagt aggtgaaaaa aacacagatg atgaagaaca cgaacgagta
     2461 gccgaacgag ccgatgtccg tgaagaacac cccgaaccgc tgcatgaatt cgctgtccgg
     2521 gaagaacgac gcgacggtgg ccggcacgaa catgatcgac tgggcgaaga tgatcggcat
     2581 gacgccggcc gcgttgacgc ggagcggcag gtactgtgtc gtgcccccgt acaccttgcg
     2641 gcccacgacg cgcttcgcat actgaaccgg gatgcggcgc atcccctgcg tcacgagcac
     2701 cacgcccgcc gtcaccagtg cgaagacgcc gatctcgagc aggaagatga acaggttgcc
     2761 cgtgagctgg agctcgttgt acatggagct cggcaggtag gcgatgatgc cgatcatgat
     2821 gatcagcgag atgccgttgc cgataccgct ctcggtgatc cgctcgccaa gccacatcac
     2881 gaacatggtc cccgcggtca gcacgatgac cgtggtgacc atgaagaacg tcgggttgat
     2941 gacgatggcc tgcccggtgg ccccgtactg gagctggatg gcgaagccga ccgactgcag
     3001 cgccgtgatg gcgatggttc cgtagcgggt cagctgggtg atcttccggc gcccctcctc
     3061 gccctcgcgc tgcagcttct ggaagtacgg gacgaccgcc cccatcagct ggatgatgat
     3121 ggacgcggtg atgtacggca tgatgccgag cgcgaagatc cccgcggcgc tgaatgcgcc
     3181 gccaacgaac aggtccagga agccgaacag gttgttcgga tccgcggtct ggttgatgtc
     3241 ggcgagcacg ctcgcgtcca cgcccggaag cgtcacgaac gcgcccagtc ggtacacgat
     3301 gaggagtccg accgtgtagg tgatccgctt ccggagctcc tcgatcttcc agatgttgcg
     3361 gagactttcg gtaaagccgg ccatgtcgga tagctgggtt tcgggagtca gtcagccacg
     3421 gtggacgatc cgccggcctt ctcgatcttg tcgcgcgcac ttgcgctgaa ggcatgggcg
     3481 gtcacctgca gcgatgcggt gatgtcgccg gagcccagga tcttgaccag gtcggccttt
     3541 ccgatcacgc cggcggcgcg caggacgtcg ggcgtcacgg gagcggcggc gtcgagggcc
     3601 cccgcgtcca cgagctgctg caggcggccc acgttcacgg gccggtattc gacgcggaaa
     3661 cggttcttga agccgaactt cggcacccgg cgctgaaggg gcatctgccc gccctcgaac
     3721 cagacgggga acttggctcc ggagcggctc ttctggcctt tgtttccctt cgtggagctg
     3781 tggccaccgt aaccggagcc ctggccccgg ccgatgcgct tctcgttgtg ggtcgagccc
     3841 tcggcgggct tcaggttgct gagatccatg tcgctgtatg cttgcgcccg ggcaggcccg
     3901 gactcgctgg cggtgcgtcg ggggcggcta ctcggccggc tccacgcgca ccaggtgatt
     3961 gaccttgtcg agcatcccgc gaagctgcgg gctctcctcg tggatgacgg tctggttcat
     4021 tcgacgaagg cccaacgcct cgagcgtgcg cttctgcgtc gcgggacgtc gaatggtgct
     4081 cctgacctgc gtaattttca actgagccat gattgggata cctggattcc gggttcgtcg
     4141 gtgcggatca gccctcgaac acccggcgga gcggaacgcc gcgccgcttg gcgacctggg
     4201 cggggtcctc gaggagcccc agggcgttca tcgtcgcgcc ca
//
LOCUS       DBNHQZ010000362         2928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2004909, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000362 DBNHQZ010000000
VERSION     DBNHQZ010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2004909"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="AC16YU_09790"
     CDS             <1..1020
                     /locus_tag="AC16YU_09790"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6065559.1"
                     /translation="SHPDARIVGALLAIALAAAFAGDAGAQDRLFRAERPAMGTTFAV
                     SLYAADPATAEATFEAAFEEIERVEQLLSDYRPTSELSRVNRLAGLEPVTVEPELQAL
                     LARVRAISEATGGAFDPTVGALVDAWGFRAGAPAVPEPHALATAMAATGMRHVRLDSA
                     ARTVRFDDARVRLDPGAFGKGYAADRAARALAASGVTRALVNAGASTFRALAPPPGET
                     GWTVTVAHGDVADTLRLVHAGLSVSGQGQRRFVSGDTTFGHILDPLVGRPAVAGRLAA
                     VRAPDALEADALSTASYVLGPGQAGLAARGRRDLSILVLPAASDPPSAYRWRWPPGPS
                     TAFLP"
     gene            1028..2245
                     /locus_tag="AC16YU_09795"
     CDS             1028..2245
                     /locus_tag="AC16YU_09795"
                     /inference="COORDINATES: protein motif:HMM:NF046806.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065560.1"
                     /translation="MQRRSFLRTMATAAAAAPLHRVAGSAFAPSDTLNVGVIGVGSRG
                     QYLMRQFLQHPGVRISALCDIYAPRLDQGRGITGEPTPGFEDYREMLAKAPDLDAVIV
                     ATPLGLHVEHDVAALEAGYHVFAEKSLALKPEGCHAIRAAAHASDRIFQVGHQYRYAG
                     WYHEAVRRIRNGDLGEVTHIYAYWHRNYNWRRPLPDPSLEHLINWRLYREWSGGLLAE
                     LGSHHIETANWIFGATPESVMGTGGIAFYRDGRETYDNVQVIYRYPGDRTLFFSSVIG
                     NQITGFQILVYGTGGTIRLTLQDGEFFYEPDRGNSAVPTGLRPPGEAASASLEAWGDM
                     PYFGPGEMLYPVEGMEASPDHLASGAFIRSVRDGTRPVANVDVGWGSGFAVAIGNEAV
                     YSGQPVRAADRMP"
     gene            complement(2262..>2928)
                     /locus_tag="AC16YU_09800"
     CDS             complement(2262..>2928)
                     /locus_tag="AC16YU_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6065561.1"
                     /translation="DAESHALHYNDMIHSFENTQEVLEVAARPTYLLESVTGAASDHD
                     APTDPFAVETEGLGRGFGRAVHRLFQAVIEHRKNPRDRAELEALADRLVREENGARSA
                     SLAERAVRAADAFRAGSIWAELEVSERVLCEFPFVHTVEDGEAPLLVSGVIDLLYRVE
                     GGWVIVDFKTEALGPRALADRYAAQLGAYRTHWTKLSGEAVEKTVIWPAVPGATSPIV
                     LKA"
BASE COUNT          415 a         1077 c          995 g          441 t
ORIGIN      
        1 tcgcatcccg acgcccggat cgtcggagcg ctcctggcga tcgcgctcgc cgccgccttc
       61 gcaggagacg cgggagcgca ggaccggctg ttccgcgcgg agcggcccgc catggggacg
      121 accttcgccg tttcgctcta cgccgccgat cctgcgaccg ccgaggccac tttcgaggcc
      181 gcgttcgagg aaatcgagcg cgtggagcag ctcctctccg actaccgtcc cacgagcgag
      241 ctctcccggg tcaaccgcct ggccggcctc gagcctgtga ccgtggagcc ggagctccag
      301 gcgctgctcg cccgcgtccg cgccatctcc gaggccacgg gcggcgcttt cgatcccacc
      361 gtcggggcac tcgtcgatgc ctggggattc cgcgccggag cgccggccgt cccggaaccg
      421 cacgccctgg cgacagccat ggccgcgacg ggcatgcggc acgtccggct cgactccgcc
      481 gcgcggacgg tccgtttcga cgacgcccgc gtgcgtctcg atcccggcgc gttcggcaag
      541 ggatacgcgg ccgaccgcgc cgcgcgggcc ctcgcggcgt cgggcgtcac gcgcgccctg
      601 gtgaatgccg gcgcgagcac gttccgggcc ctcgcgcctc cgcccggcga gacgggctgg
      661 acggtgaccg tggcgcacgg tgacgtcgcc gacacgctgc ggctcgtcca cgcgggactc
      721 tccgtgtcgg gccaggggca gcgccgcttc gtctcgggcg acaccacgtt cggccatatc
      781 ctcgacccgc tcgtcggacg tccggccgtc gcgggccgcc tggccgccgt ccgggcgccc
      841 gacgccctcg aggcggacgc actgtccacc gcatcgtatg ttctgggacc ggggcaggcc
      901 ggcctggcgg cccggggccg gcgggacctg tcgatcctgg tgctgccggc cgcgtccgac
      961 cctccgtccg cctaccggtg gcgctggcca cccggcccgt cgaccgcctt ccttccctga
     1021 ccgcagcatg caacgccgat ccttcctccg cacgatggcc accgcagcgg cggccgcacc
     1081 cctccatcgc gtggccggca gcgccttcgc accgtcggac acgctcaacg tgggcgtcat
     1141 cggggtcgga tcccgcggac agtacctgat gcgccagttc ctccagcacc ccggggtgcg
     1201 gatctcggcg ctctgcgaca tctatgcgcc caggctggac cagggccggg gaatcacggg
     1261 cgagccgact ccgggcttcg aggactatcg cgagatgctc gccaaggcgc cggacctgga
     1321 cgccgtgatc gtggcgactc cgctgggcct gcacgtggag catgacgtgg ccgcgcttga
     1381 agccggatat cacgtgttcg ctgagaagtc gttggcgctc aagcccgaag ggtgccacgc
     1441 catcagggcg gcagcgcacg cgagcgaccg catcttccag gtcgggcacc agtaccgcta
     1501 cgcgggatgg taccacgagg ccgtgcggcg catccgcaac ggggacctgg gcgaagtcac
     1561 ccatatctac gcgtactggc accggaacta caactggcgg cggcccctgc ccgacccgtc
     1621 gctcgagcac ctcatcaact ggcgcctgta ccgggaatgg tcgggaggac tgctggccga
     1681 gctcggatcg caccacatcg agacggccaa ctggatcttc ggggccactc cggagagcgt
     1741 gatggggact ggcggcatcg cgttctaccg cgacgggcgg gagacgtacg acaacgtcca
     1801 ggtgatctac cggtatccgg gcgatcgcac gctcttcttc agttcggtca tcggaaacca
     1861 gatcacggga ttccagatcc tggtctacgg cacgggcggc acgatccggt tgacgctcca
     1921 ggacggggag ttcttctacg agccagaccg cggtaattcg gccgttccga cggggctccg
     1981 tccgccgggc gaggccgcaa gcgcctcgct ggaagcctgg ggtgatatgc cgtacttcgg
     2041 accgggcgag atgctctacc ccgtcgaggg catggaggcg agccccgatc accttgcttc
     2101 gggggcgttc atacgctcgg tgcgcgacgg cacgcgcccc gttgccaacg tggacgtggg
     2161 atggggttcc gggttcgcgg tcgcgatcgg caacgaggcc gtctattcgg ggcagcccgt
     2221 gcgagccgcg gaccgcatgc cttgattggc cccggccccg cctaggcctt cagcacgatc
     2281 gggctcgtcg cgccggggac ggccggccag atgacggtct tttcgaccgc ctccccgctc
     2341 agcttcgtcc agtgggtgcg gtaggcgccc agctgggcgg cgtaccgatc cgcgagggcc
     2401 cgcggcccga gcgcctccgt cttgaagtcc acgatcaccc agccaccctc cacccggtac
     2461 agcaggtcga tcacgcccga gacgagcagc ggcgcctccc catcttcgac ggtgtggacg
     2521 aacgggaact cgcagagcac ccgttccgac acttcgagct ccgcccatat cgaaccggcg
     2581 cgaaaggcgt ccgccgcccg cacggcgcgc tcggccagtg acgcagagcg cgctccgttc
     2641 tcctcccgta ccaggcggtc ggcgagcgcc tcgagctccg cccggtcccg cggattcttc
     2701 ctgtgctcga tgacggcctg gaacaggcgg tgcaccgcgc ggccgaagcc gcggccgaga
     2761 ccctccgtct cgaccgcgaa cgggtcggtc ggggcgtcat gatccgacgc ggcccccgtg
     2821 accgattcca gcaggtaagt aggccgagcg gccacctcga ggacctcctg cgtattctcg
     2881 aaggaatgta tcatgtcgtt gtagtgcaac gcatgagact ccgcatcg
//
LOCUS       DBNHQZ010000363         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1869816, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000363 DBNHQZ010000000
VERSION     DBNHQZ010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1869816"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="AC16YU_09805"
     CDS             complement(<1..1403)
                     /locus_tag="AC16YU_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6065562.1"
                     /translation="MKASASAILLLVVVVSGACRSGPPERTTPVFRNVSGEAEYVGNG
                     ACVACHEAQFAGFAEHGMGRSMYALTPERRVETFPGPSVVDATTGLRYRALLEDGVPV
                     QEETLEDDAGRIVHRLVRPMEFVMGSGNAARTYFFSDGGWMMQLPLTWYTQAGRWDFS
                     PGYDVANGRFGRTLPERCMACHNGVPEAVPQTNGKYASFPLGITCERCHGPGSVHVDE
                     RLATPEPDGPVDVTIVNPRHLAFDRRMDVCQQCHLNGTVSVLREDRAPYGYLPGEPLA
                     AHIAVFSEPASGSGIPVISHVERLEASACFRGAGGSLECTTCHDPHAASPGPATFGGV
                     CLDCHDGALPQNPPHDDPASCTTCHMPVVDADEAPHSSFTDHRIRVVRAAGDEAVTAT
                     SVGRLAPYFEAEDDEARGMALVVLGRQRGERSLVEAGRDVLAGLFPDLGGEAKLLLGL
                     AWLDTGDADSAISPLEAA"
     gene            complement(1527..>2279)
                     /locus_tag="AC16YU_09810"
     CDS             complement(1527..>2279)
                     /locus_tag="AC16YU_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6065563.1"
                     /translation="NYFGLAQRFGSSNLALTLSSWDFGDIPLQTEDSPDISDVTWTAR
                     YTTFALAFAREFTDRISAGVNVKMVSETIDDVSASGVAFDAGMTYAVGESGLRFGVSL
                     KNFGPDRNYSGTGMVRFVQLSEQIPDAQPQAVQLEGARYELPSLLNFGVAYTRPLGDA
                     MAVTVLGNFRSNSFSQDQYAGALELGFKEILFVRGGYEFQSDMDQTFWKGTNIGAGLV
                     VPLGSNGLEIDYAYRDTDFFDAVHMWTVGITL"
BASE COUNT          387 a          782 c          768 g          342 t
ORIGIN      
        1 gctgcctcga gcggcgagat cgcagaatcc gcgtctccgg tgtccagcca cgccagaccg
       61 agcaggagtt tcgcctcccc gccgaggtcc gggaacagcc cggcgagcac gtcccggcct
      121 gcctcgacga ggctcctctc gccccgctgc cggcccagga ccacgagggc cattccgcgc
      181 gcctcgtcgt cctcggcctc gaagtacggg gcgagccgcc cgaccgacgt cgccgtcacg
      241 gcttcgtcgc ccgcggcgcg cacgacgcgg atccggtggt ccgtgaagga cgagtggggc
      301 gcttcgtccg cgtccactac cggcatgtgg caggtggtgc aggaggcggg gtcgtcgtgg
      361 ggtggattct ggggaagcgc cccgtcgtgg cagtccaggc agaccccgcc gaacgtggcc
      421 gggccggggc tggccgcgtg gggatcgtgg caggtcgtgc attcaagcga tccgccggcc
      481 ccgcggaagc acgcgctcgc ctcgagccgc tccacgtgcg atatgaccgg gatgccgcta
      541 ccggaggccg gctcggaaaa gaccgcaata tgtgcggcca gcggctcccc gggcaggtag
      601 ccgtagggcg cccggtcctc ccgaagcacc gataccgtgc cgttcagatg gcactgttga
      661 caaacgtcca tgcgccgatc gaacgccagg tggcgcggat tcacgatggt gacgtccacc
      721 gggccgtccg gctcgggagt cgccaggcgc tcgtcgacgt gaacgctgcc ggggccgtgg
      781 caccgttcgc acgttatgcc gaggggaaac gacgcgtact tcccgttggt ctgcgggacg
      841 gcctcgggca ccccgttgtg gcaggccatg cagcgttccg gcagggtccg tccgaagcgc
      901 ccgttggcga cgtcatatcc cggggaaaag tcccacctgc ccgcctgggt gtaccaggtc
      961 aggggcagct gcatcatcca cccgccgtcc gagaagaagt acgtccgggc cgcgttgccg
     1021 gagcccatca cgaactccat cggacggacc aggcggtgca cgatccgccc ggcgtcgtcc
     1081 tcgagggtct cctcctgcac cgggactccg tcctcgagca acgcccggta ccggagcccg
     1141 gtcgtcgcgt cgaccactga cggccccgga aacgtctcta cccggcgttc cggggtgagc
     1201 gcgtacatgg atcggcccat cccgtgctcc gcaaacccgg cgaactgcgc ttcgtggcag
     1261 gccacgcagg ccccgttgcc aacgtactcg gcctctcccg acacattcct gaagacgggc
     1321 gtcgtgcgtt cgggcggtcc ggagcggcag gcgccggaga ccacgaccac gaggaggagt
     1381 atcgccgaag cggacgcttt caccgcatcg aaagaggcac tgtggtcacc tggggggcac
     1441 ggccaaacag aacggggtga gcctcgtcga ggcccacccc gtcccgcccc ggcccaaggc
     1501 cggagccgat aaagctcgcc gtgctcctaa agggtaatcc cgaccgtcca catgtggacc
     1561 gcatcgaaga aatccgtgtc gcggtaggca tagtcgatct ccagcccgtt ggagccgagc
     1621 ggcacgacca ggccggcacc gatgttcgtc cctttccaga acgtctggtc catgtcggac
     1681 tggaactcgt agccgccccg gacgaagagg atctccttga agccgagttc cagggccccg
     1741 gcgtactgat cctgcgagaa cgagttggag cggaagttgc cgagcaccgt cacggccatc
     1801 gcgtcaccca gtggccgggt gtacgcgacg ccgaaattca gcaacgacgg aagctcgtac
     1861 cgggccccct cgagctgaac ggcctggggc tgggcatccg gaatctgctc gctgagctgc
     1921 acgaagcgga ccatgccggt gccggagtag ttgcggtccg gaccgaagtt cttcaggctg
     1981 acgccgaagc gcagtcccga ctcgccgacg gcatacgtca tgccggcgtc gaacgcgacg
     2041 cccgacgcgc tcacgtcgtc gatggtctcg ctgaccatct tgacattgac accggcagag
     2101 atcctgtcgg tgaactcgcg ggcgaacgcc agggcaaacg tcgtgtacct ggcggtccag
     2161 gtgacgtcgc tgatgtccgg cgaatcctcg gtctgcaacg gaatgtcacc gaaatcccag
     2221 gaactgagcg tcagcgcaag gttcgaagag ccgaagcgct gggccaggcc gaagtagtt
//
LOCUS       DBNHQZ010000364         6957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3224119, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000364 DBNHQZ010000000
VERSION     DBNHQZ010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3224119"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="AC16YU_09815"
     CDS             complement(<1..243)
                     /locus_tag="AC16YU_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065564.1"
                     /translation="MPGRLAVAPLLLVLFLTACDSPTGVGSDLVDEQGGNPVEFAFDP
                     VEMRIADEADVTGGSASIQRMLSGVTADPYLGLIQAT"
     gene            complement(406..1167)
                     /locus_tag="AC16YU_09820"
     CDS             complement(406..1167)
                     /locus_tag="AC16YU_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR6065565.1"
                     /translation="MRILFVSGEVAPFTTKSEIASLSRHLPDQLHETGRFETRIMMPR
                     YGIVSERRNRLHEVIRLSDNDIPMGAQSESLKVKVASIPGIRLQVYFMDNARLFKRKG
                     VFADKSGKVFEDNQERAAFFGRAVIETIRNLGWDPDLVHTFGWMSGLVPFMLRSVYAD
                     DPLFANSRIVHTPYPVEFEAPYTREMLEGFGLPAHKDAVDVDANAIGRAYADAVILPG
                     GVVSENGLDQFGEDHEGNREIASAVYERVLASVAA"
     gene            1474..1947
                     /locus_tag="AC16YU_09825"
     CDS             1474..1947
                     /locus_tag="AC16YU_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIR6065566.1"
                     /translation="MITLEPRPVILFDGFCNLCSASIDFVIGRDPRGHFLLAPLQSEA
                     GNNLLRRHGRSAGAPDTVILIERGRLYERSTAALRIARRLRGAWPILYAFILVPRFLR
                     DPVYDWIARNRTRWFGRRETCRVATPDVAARFLGSPVQPPVESGGTAPPEPAASC"
     gene            complement(1890..5441)
                     /locus_tag="AC16YU_09830"
     CDS             complement(1890..5441)
                     /locus_tag="AC16YU_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HIR6065567.1"
                     /translation="MTPLPSDPIGRIRRHLADARRRLALGDILSGALWFAGVAAAAWV
                     TAAALEAVLWLPSGVRAAMLAVVAAGSLAFLLRMVVWPALRWSGLVRGISDDTVAGQV
                     GRRLPEVRDRLLNLLQLLDGRHSDAPEPLVRSAADHLGIRLQDVDLASVEDFAGARRA
                     GRLASLPLVGLLAFVAIAPGAFFGASSRLLAPARDFSRPAPYAVEVGPGDIELVKGAD
                     LAVTTRISGSGIPGTAVLETRYPDETVTERLELPLAGGEARHTLVNVRRTTEYRVSAG
                     SVASPWFTARVVDRPVVGNLQVVLRYPAYTRMPPLALAPNEGDITALAGTEVLAEAVV
                     GGPPIREARLVFGRAAVRPLELDGRMAAGSFRVTGDDTWHVEAVSDDDVANLDPVVHD
                     IRRVEDARPSVAVTSPESEFDLDESASVPLAWRISDDFGFSALRLWYRLESSRFGTPA
                     DAYEAVDLPLERPAPREQDGGMNWLLRERTSIDPAPGDVIVYYLEVTDNDAVSGPKSS
                     RTEEYRLRLPSLVERYEALDETEDTVEDDLEEVKDEATRMQEDFDELQEQLRGKPEAD
                     WQDERAVERLQQRQESMEESVREIADRIESMTEEMERNDLVGEETLEMFRELQEVAEE
                     INSPELMEALQELQEAMQQMDLQRMQEAMEDFRFNEEQYRQRLERTLELFRNLRVQQD
                     LDEAARRAEELARTEEELARRTEELEREKSPDEEKPKAEESGDGDEEKPKAEESGDGD
                     KQEGDSAGEDTPPENEQLAREQERAAEEMEKLEEMLRELQERMEGLRQAPSESMQNLS
                     KQTESQQMPQQMRQNAGQLQNQQLAPAKQSQQEMSEQLRQLQQQLQQMLSGMQGSQME
                     VNLSGLRRALDDVLALSEDQEALRAEVQVLADESPGLRESARRQVQLGEGLNMVSDSL
                     QSLARQIPQMGRDIQTHAGEALREMADATQSMTDRTGRRAVGHQKGAMTQLNELALLL
                     ADLMNQMMGGGGGSGSMSMQQMLQQLQNMAGQQQMLNQQMQQLLNDIRGNRLSSNAQE
                     RLRQLGEQQQRIREQLRDLNRNRELRNQALGDLERIAEQMQETIEELTLRQVTPRTIE
                     RQRQILTRLLEASRSLQERGRDRRRESRTGEDILRQGPPELTAPERADQLRRDLIRAL
                     ESGYAPDFEELIRRYFELLQQDAAGSGGAVPPDSTGG"
     gene            5615..6883
                     /locus_tag="AC16YU_09835"
     CDS             5615..6883
                     /locus_tag="AC16YU_09835"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR6065568.1"
                     /translation="MNATTAIILGGGAGTRLFPLTQHRSKPAVPLAGKYRLIDIPISN
                     CINSGISRIFVLTQFNSASLNRHISQTYLFDRFRHGFVNILAAEQTPQSKDWFQGTAD
                     AVRQSMRHVESYRFENTLILSGDQLYTMDYKLMVEHHRDTGADLTIATIPVHANDAPG
                     FGILKTDEDGVISEFHEKPSRQDLAGKESRVNAEMQAQGRVYLASMGIYVFSKGILQE
                     LLEESPDAHDFGKQIIPRSMGMKRVVSYAFDGYWSDIGTIRSFFDANLMLTQKQPQFD
                     LYNPRMPLYTNARMLPPAKIESSYVVDSIIAEASLVVNSQISNSVIGIRSVIGNNTTI
                     KNTVIMGSDYFPWHDPELRESVEGPLRPGIGEESYVEGAIIDRNVSVGKRCIIKNREN
                     IDRFDGDNYYIRDGIVVIPKNAHIPDDTII"
BASE COUNT         1094 a         2368 c         2308 g         1187 t
ORIGIN      
        1 cgttgcctgg atgaggccga gatacgggtc ggccgtgacg ccggaaagca tgcgctggat
       61 cgaggcggaa ccgcccgtca cgtcagcctc gtccgcgatg cgcatctcga ccggatcgaa
      121 tgcgaactcc accgggttgc cgccctgttc gtccacgagg tcggacccca caccggtggg
      181 actgtcgcag gcagtgagga agagaaccag aagcagcggt gcgaccgcga ggcgtcccgg
      241 catggcgtca ggagagaggg tgatgcgagc caaacggccg gacggggtga cttcgtatgg
      301 gagtcggaca aacgaaaacc ccatccgacg gcgtcgctgg ggtttcgcgt tcagtcccgg
      361 gctatatgca ccggcccgtg ggaggtttcc gacgggccgg gcggatcagg cggccacgga
      421 cgccaggacc cgttcgtaca cggcggatgc gatttccctg ttgccctcgt ggtcctcgcc
      481 gaactggtcg aggccgttct ccgagaccac gccgcccggc aggatcaccg cgtccgcgta
      541 cgcccggccg atggcattgg catcgacgtc cacggcgtcc ttgtgggccg ggaggccgaa
      601 gccctcgagc atctcccggg tatagggcgc ctcgaattcc acggggtacg gcgtgtggac
      661 gatccggctg ttcgcgaaga gcgggtcgtc ggcgtagacg ctccgcagca tgaacggcac
      721 gagaccgctc atccagccga acgtgtggac caggtcggga tcccagccca ggttccggat
      781 ggtctcgatg acggcccggc cgaagaacgc cgcgcgctcc tggttgtcct cgaagacctt
      841 gccggacttg tccgcgaaga cgcccttgcg cttgaacagc ctcgcgttgt ccatgaaata
      901 gacctggagc cggatgcccg gaatggaggc caccttgacc ttgagcgatt cggactgcgc
      961 ccccatgggg atgtcgttgt ccgacagccg gatcacctcg tgcagccgat tcctgcgctc
     1021 ggagacgatc ccgtagcggg gcatcatgat ccgggtctcg aagcggcccg tctcgtgcaa
     1081 ctggtccgga aggtgccgcg agagggacgc gatctcggac ttggtggtga acggggcgac
     1141 ctcgcctgaa acgaacagga ttctcattag gtgtcgacgc ttatggaacg ggaccgggag
     1201 gcccgcaggg cccgggaacg attttgctct cggccgctca tgccgagccg tgaaggtcca
     1261 cgctgcggaa aggacacggc gtcgccggcg tcgcaggccc tctcctcacc gcggtgtcac
     1321 gaagttctct gcgtatcctg ctgtcctgct gcacaggatc agtacccaaa tttacaaatt
     1381 atgtggctgt tttttcaagc ttaatcgaac gggatgcgaa cgacggccga cggcggggga
     1441 gccgcgacgt tgcgccggcg taaccagatg atcatgatca ccctcgaacc tcgccccgtc
     1501 atcctgttcg acggcttctg caatctgtgc agtgcgagca tcgatttcgt gatcggacgc
     1561 gacccgcgag ggcacttcct tttggccccc ctgcagtcgg aggcggggaa caacctgctc
     1621 cggcggcacg gccgttcggc tggggccccg gacaccgtga tcctcatcga gcggggccgt
     1681 ctgtacgaac gatccaccgc cgcgctccgc atcgcgcgac gcctccgtgg agcgtggccg
     1741 atcctttatg ctttcatcct ggttccccga ttcctgcgcg atccggtcta tgactggatc
     1801 gcccgtaacc gcacccgctg gttcggccgg cgggagacct gccgggtcgc gacgcccgac
     1861 gtggccgccc ggtttctcgg gagcccggtt cagccgcccg tggaatcggg cgggacagcc
     1921 ccgcccgaac ccgccgcgtc ttgctgaagc agctcgaagt accggcggat gagttcctcg
     1981 aagtcagggg catagccgct ctcgagcgcc cggatcaggt ctcggcgaag ctggtccgcc
     2041 cgctccggcg ccgtgagctc gggaggcccc tgccgaagga tgtcttcgcc ggtccggctc
     2101 tcgcggcgac ggtcccggcc ccgctcctgc agggatcggc tggcctcgag cagtcgggtc
     2161 aggatctggc gctgccgctc gatggtgcgc ggggtgacct gtcgcagggt gagctcctcg
     2221 atggtctcct gcatctgctc cgcgatgcgc tccaggtccc cgagtgcctg gttccgcagc
     2281 tcccggttcc ggttcaggtc gcgcagctgc tcgcggatgc gctgctgctg ctcgcccagc
     2341 tgccgcagcc gctcctgggc attcgagctc agccggttgc cgcggatgtc attgagaagc
     2401 tgctgcatct gctggttgag catctgctgc tggccggcca tgttctgcag ctgctggagc
     2461 atctgctgca tggacatgga tccgctgccg ccacccccgc ccatcatctg gttcatgagg
     2521 tccgccagga gaagggcgag ctcgttcagc tgcgtcattg cgcccttctg gtggccgacg
     2581 gccctgcgcc cggtccggtc ggtcatcgac tgcgtagcgt cggccatctc gcggagcgcc
     2641 tcaccggcat gggtctgaat gtcacggccc atctgcggaa tctgccgggc gagggactgg
     2701 agggaatcgg ataccatgtt caggccctcg ccgagctgca cctggcgccg ggcggactca
     2761 cgcaggcccg gcgattcgtc ggccagcacc tggacctcgg cgcgcagcgc ttcctggtcc
     2821 tcggacaggg cgagcacgtc gtccagggcg cgtctcagcc cggacaggtt cacctccatc
     2881 tgtgatccct gcatgcccga gagcatctgc tgcagctgtt gctggagctg ccgcagctgc
     2941 tcgctcatct cctgctgcga ctgcttggcc ggcgcgagct gctggttctg cagctgcccc
     3001 gcgttctgcc gcatctgctg cggcatctgc tgggactcgg tctgcttcga gagattctgc
     3061 atcgattcgg agggggcctg cctgaggccc tccatccgct cctggagctc gcggagcatt
     3121 tcctcgagct tctccatttc ctcggccgcc cgctcctgct cgcgggcgag ctgctcgttc
     3181 tctggcggcg tgtcctcgcc ggcggagtcg ccctcctgct tgtcgccgtc cccggattct
     3241 tcggctttcg gcttctcctc gtcgccgtcc ccggattctt cggccttcgg cttctcctcg
     3301 tccggtgact tctcccgctc gagctcctcg gtgcgcctgg cgagttcctc ttcggtccgg
     3361 gcaagttcct cggcacgccg cgccgcctcg tccaggtcct gctgcacccg caggttgcgg
     3421 aagagctcca gcgtccgctc gaggcgctgc cggtactgct cctcgttgaa gcggaaatcc
     3481 tccatggctt cctgcatccg ctgcaggtcc atctgctgca tggcctcctg gagctcctgc
     3541 agggcctcca tcagttccgg cgaattgatc tcctcggcca cctcctggag ctcacggaac
     3601 atctccaggg tctcctcgcc cacgagatcg ttccgctcca tctcctcggt catggactcg
     3661 atccggtcgg cgatctcccg gaccgactcc tccatggatt cctggcgctg ctgaagccgc
     3721 tcgaccgccc gctcgtcctg ccagtcggcc tccggcttgc cgcggagctg ctcctggagt
     3781 tcgtcgaagt cctcctgcat gcgcgtggcc tcgtccttca cctcctcgag gtcgtcctcc
     3841 acggtgtcct ccgtctcgtc cagcgcctcg tagcgctcaa cgagcgaggg caggcgcagg
     3901 cggtactcct ccgttctcga cgacttgggg ccggaaaccg cgtcgttgtc cgtcacctcg
     3961 aggtagtaca cgatgacgtc gccgggcgcg ggatcgatgg atgtgcgctc ccgcagcagc
     4021 cagttcatcc ccccgtcctg ctcacgggga gcgggccgtt cgaggggcag gtcgaccgcc
     4081 tcgtacgcgt ccgcgggcgt gccgaatcgc gaggattcca ggcgatacca caggcggagc
     4141 gccgagaagc cgaagtcgtc ggagatccgc caggcgagcg gcaccgaggc ggattcgtcc
     4201 aggtcgaatt cggactccgg agacgtgacg gccaccgacg gacgggcatc ctcgacgcgg
     4261 cggatgtcgt ggacgacggg gtcgaggttg gccacgtcgt cgtctgatac ggcctcgaca
     4321 tgccaggtgt cgtctccggt gacgcggaac gagcctgctg ccatgcggcc gtcgagctcg
     4381 agcggacgaa ccgccgcgcg gccgaagacg agccgggcct cccggatcgg ggggccgccc
     4441 acgaccgcct cggcgagcac ttcggtgccg gcgagggcgg tgatgtcacc ctcgttgggc
     4501 gccagtgcca gcggaggcat gcgcgtgtaa gccgggtagc ggagcaccac ctgcaggttg
     4561 ccgacgacgg gcctgtccac cacgcgcgcc gtgaaccagg gcgacgcgac ggagccggcc
     4621 gatacgcggt actcggtggt gcggcggacg ttgaccagcg tgtggcgcgc ctcgccgccc
     4681 gcgagcggga gctcgaggcg ctcggtcacg gtctcgtccg ggtagcgggt ctcgaggacc
     4741 gcggtcccgg gaatgcccga cccgctgatc cgggtcgtga cggcgaggtc cgcgcccttg
     4801 acgagttcta tgtctcccgg acctacctcc accgcgtacg gggcgggtcg gctgaagtca
     4861 cgggccgggg cgaggagcct ggacgacgcc ccgaagaacg cgcccggcgc gatggctacg
     4921 aacgccagaa ggccgacgag cggaagcgag gccagccgcc cggctcggcg cgccccggcg
     4981 aaatcctcga cggaagcgag gtcgacgtcc tgcagcctga ttcccaggtg atccgcggcc
     5041 gagcggacca gcggctcggg cgcgtcggag tgccgtccgt ccaggagctg caggaggttc
     5101 agaagccggt cccgtacctc cggcagcctg cgtcccacct gtccggcgac cgtatcgtca
     5161 ctgatgccgc gcacgagtcc ggaccaccgg agggcgggcc aaaccaccat gcggagcagg
     5221 aacgccaggg agcctgccgc cacgacggcg agcattgccg cgcggacccc ggaaggcagc
     5281 cacagcacgg cctccagcgc ggcggccgtc acccacgcgg cggcggcgac gcccgcgaac
     5341 cacagcgcgc ccgagaggat gtcccccagg gccagccgtc gccgcgcgtc ggccaggtgt
     5401 cggcggatgc gtccgatcgg atcggatgga aggggggtca tgcgggtcca ggccggggga
     5461 gcagaggggt gtacacggcg cacggacgcg ggtttctccc gtcagcagga ggtcacgccg
     5521 cccgcccata cttgaacggc ccggggagcc accgtattgt tcccgcggat ccggaagggg
     5581 ccttccggga ccgggacgca cacgccagca tccgatgaac gccacgactg ccatcatcct
     5641 gggcggtggg gccggaaccc gcctgtttcc gcttacccag cacagatcca agcctgccgt
     5701 cccgctggcc ggcaagtacc ggctgatcga catccccata tcgaactgca tcaactcggg
     5761 catcagccgc atcttcgtgc tgacgcagtt caactcggcg agcctgaacc gccatatttc
     5821 ccagacctac ctgttcgacc ggttccgtca cggattcgtg aacatcctcg ccgcggagca
     5881 gaccccgcag tcgaaggact ggttccaggg cacggccgac gcggtccggc agagcatgcg
     5941 tcacgtcgag tcgtaccggt tcgagaacac gctgatcctg tccggcgacc agctctacac
     6001 catggactac aagctcatgg tggagcatca ccgcgacacg ggggccgacc tgaccatcgc
     6061 gacgattccg gtccacgcga acgacgcccc ggggttcggc atcctgaaga cggacgagga
     6121 cggagtcatc agcgagttcc acgaaaagcc ctcgcggcag gacctcgcgg gcaaggagag
     6181 ccgcgtgaac gccgagatgc aggcccaggg ccgcgtctac ctcgcgtcga tgggcatcta
     6241 cgttttctcc aagggcatcc tgcaggagct gctggaggag tcgccggatg cgcacgactt
     6301 cggcaagcag atcatccccc gttccatggg gatgaagcgg gtggtgagct acgcgttcga
     6361 cgggtactgg agcgacatcg ggaccatccg ctcgtttttc gacgcgaacc tcatgctcac
     6421 gcagaagcag ccccagttcg acctgtacaa tccgcggatg ccgctgtaca cgaacgcgcg
     6481 catgctgccg ccggctaaga tcgagagctc gtatgtagtg gactcgatca tcgcggaggc
     6541 gagcctcgtg gtcaacagcc agatatcgaa ctcggtcatc gggatacgct cggtcatcgg
     6601 caacaacacg acgatcaaga ataccgtgat catggggtcg gactacttcc cgtggcacga
     6661 cccggagctc cgcgaatcgg tggaggggcc gctccggccg gggatcgggg aggagtccta
     6721 cgtggagggc gcgatcatcg accgcaacgt atcggtcggc aagcggtgca tcatcaagaa
     6781 ccgcgagaac atcgatcgct tcgacgggga caactactac atccgggacg gcatcgtggt
     6841 gattccgaag aacgcgcaca ttccggacga cacgatcatc tgacctcttc ccgatggccg
     6901 cgggtggaaa cccggacggt tccgcggcgt gcgacgccgg accaaccgaa atccgag
//
LOCUS       DBNHQZ010000365         4219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1871188, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000365 DBNHQZ010000000
VERSION     DBNHQZ010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1871188"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="AC16YU_09840"
     CDS             <1..310
                     /locus_tag="AC16YU_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065569.1"
                     /translation="LKIEYQITPAIFVRFIGQYTDRERDRLLDRGGSPILVDGAPDPA
                     QEEGIFQTDWLFSYRPIPGTLLYVGYSSVLEQLENARPGVLQREVDGFFAKISYTIRT
                     "
     gene            complement(313..2004)
                     /locus_tag="AC16YU_09845"
     CDS             complement(313..2004)
                     /locus_tag="AC16YU_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6065570.1"
                     /translation="MTPTPRSPVFEPKLISVWKEGYTWKLFQHDAVAGLVVGVVALPL
                     AIAFAIASGVGPERGLYTAIVAGALISILGGSRVQIGGPTGAFVVLVYAIVQSHGFDG
                     LVVATILAGLLLVAMGLARLGQVISFIPYPVTVGFTSGIAIIIGLGQVRDFLGLDMEA
                     VPAEFFSRLGAYADAVGTVSPVTVAIAVGSVLVMRLWPHVTHRVPGSLVALLAATVAV
                     WALDLEVQTIGSRFGAVPSHLPVPSLPDIDLSIVPDLLPAALSIALLGGIESLLSAVV
                     ADGMTGRRHRSNTELIAQGIANVVTPLFGGIPATGAIARTATNIKSGGRTPVAGIVHA
                     VVLLVILLLLAPLAAEIPMATLAGILMVVAWNMGEWHVFRRLLRSPRSDVLVLLSTFV
                     LTVVIDLTIAIQVGVVLAALLFMHRMAQASEFGFVRADDEREPDEVELPPLPAGVVVF
                     EVNGPFFFGAADRFRHAMSQIEEPPRVIILRLRQVPAIDATAMTALADVVERTKNAGG
                     AIVLSGARSGPLEHLRRAGVVARVGKANVVPDFEAAVRRAHAIVERTNGAGAADA"
     gene            2219..3100
                     /locus_tag="AC16YU_09850"
     CDS             2219..3100
                     /locus_tag="AC16YU_09850"
                     /inference="COORDINATES: protein motif:HMM:NF016816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR6065571.1"
                     /translation="MRLRILLLPILLVPLVVRAQDRPLRVYISVDMEGIGGIGTSAMT
                     SASGKDYALGRELMTAEVNTVVEAVFGHGEAEVLVNDSHGDMQNLLHERLDPRAQYIQ
                     GNIKPLGMVQGLDRAFDAAVFLGYHARAGTPDAFLAHTGSGSVKGVWIDGVEVGEGGM
                     NALFAASHGVPVIVASGDRAFAAQFAERVPGAMTVVTKEAVGSMVARLDHPEVVRARL
                     REATSRALAGLAALPEPQDPGQVTVRMRFATTTRPDIAMAVPGMRRIDGYTVEFDAPD
                     MSAAYPIVRLLYKYVSW"
     gene            3197..>4219
                     /locus_tag="AC16YU_09855"
     CDS             3197..>4219
                     /locus_tag="AC16YU_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR6065572.1"
                     /translation="MRDLRPVPAFVAGAGMIAGSLLTIRHYFEANFPATIFEGAFCDI
                     SAFINCDSSAYSGIAAVQGVPIGTFGMMLGALVVLASLFASWRLAKTVRFLAALNVAG
                     VVALLAWSVVGLGSLCLLCAIYYAFSFLALWAAWPGPPGANRFAPNPRVLAVAGAATL
                     AVALGMSAYHEARVQARSGGNAARVVAQFRSLPVVDPPSVLSPNWIVRSTERFEDAPL
                     RIVAWEDFLCSDCLYLARQIERLKEEYGARMNLVFQHFPLDAACNGVVEKDKHPGACE
                     LSLMAAYAPERFDEIYEAVYADQRAAKDPGWRRALAARLGVEAAAGDSAARAVVAASI
                     ATGREYE"
BASE COUNT          619 a         1477 c         1507 g          616 t
ORIGIN      
        1 gctcaagatc gagtaccaga tcacgccggc gatcttcgtc cggttcatcg gccagtacac
       61 cgaccgcgag cgcgaccggc tcctggaccg cggcgggagc ccgatcctcg tggacggcgc
      121 acccgacccg gcgcaggagg agggcatctt ccagacggac tggctcttca gctaccgacc
      181 gatcccgggt acgctgctct acgtcggata cagctccgtg ctggagcagc tcgagaacgc
      241 gcgcccgggc gtgctgcagc gcgaggtgga cggcttcttc gccaagatca gctacaccat
      301 ccgcacctga cgttacgcgt ccgcggcccc ggctccgttg gtcctctcga cgatggcgtg
      361 ggcgcgccgg acggcggcct cgaaatccgg gaccacgttg gccttgccga cgcgggccac
      421 gacccccgcc cggcgcaggt gctcgagcgg gccgctgcgg gctcccgaaa gcacgatcgc
      481 cccgcccgcg ttcttcgtcc gctcgaccac gtcggcgagc gcggtcatcg ccgtggcgtc
      541 gatcgccggg acctgccgca ggcgcaggat gatgacgcgg ggcggctcct cgatctgact
      601 catcgcgtgc cggaagcggt ccgcggcccc gaagaagaac ggcccgttga cctcgaagac
      661 gacgacgccc gcgggcagcg ggggcagttc gacctcgtcc ggttcccgct cgtcgtcggc
      721 ccggacgaag ccgaactcgc tcgcctgcgc catgcggtgc atgaagagca gggccgcgag
      781 caccacgccc acctggatgg cgatcgtgag gtcgatcacg acggtcagca cgaaggtcga
      841 cagcagcact agcacgtcgc tgcgcgggct ccggagcagt ctccggaaga cgtgccattc
      901 gcccatgttc caggccacca ccatgaggat tcccgccagc gtggccatcg gaatctcggc
      961 cgccaggggc gcgagcagga gcaggatgac caggagcacg acggcatgga cgatgcccgc
     1021 caccggcgtg cgccccccgc tcttgatgtt cgtggccgtc cgcgcgatcg ccccggtggc
     1081 cgggatgccc ccgaagagcg gcgtgacgac gttggcgatg ccctgcgcga tgagctcggt
     1141 gttggagcgg tgccggcggc cggtcatgcc gtcggcgacc acggccgaga gcagcgactc
     1201 gatgccgccg agcagcgcga tcgagagggc cgccgggagc aggtccggca cgatggacag
     1261 gtcgatgtcc gggaggctgg gcacggggag gtgcgagggc accgccccga agcgcgaccc
     1321 gatcgtctgg acttcgaggt cgagcgccca tacggccacg gtcgcggcca ggagggcgac
     1381 gagggacccc ggcacgcggt gcgtcacgtg gggccagagc cgcatcacga gtaccgagcc
     1441 gaccgcgatc gccacggtga ccggcgacac ggttccgact gcgtccgcgt acgccccgag
     1501 ccggctgaag aactccgccg gcaccgcctc catgtccagg cccaggaagt cccggacctg
     1561 ccccaggccg atgatgatcg cgatgcccga cgtgaagcct acggtcaccg ggtaggggat
     1621 gaacgagatg acctgcccga ggcgcgcgag ccccatcgcc acgagcagga ggccggcgag
     1681 gatggtcgcg acgacgagcc cgtcgaagcc gtggctctgg acgatcgcgt agacgaggac
     1741 cacgaaggcg cccgtcggcc cgccgatctg cacgcggctg cccccgagga tcgagatcag
     1801 ggctcccgcg acgatcgccg tgtacagccc ccgctcgggg cccacgccgg aggcgatcgc
     1861 gaatgcgatg gcgagcggga gcgcgaccac gccgaccacg agccccgcca cggcgtcgtg
     1921 ctggaacagc ttccaggtgt agccctcctt ccagaccgag atgagcttgg gttcgaagac
     1981 cggggagcgg ggcgtcgggg tcaaggccgg gcgaagggat ggaagcgggc gccggggcgg
     2041 ccgtctgccg tcggcgggcg gttgaaccac cgggccgtgc cgtgttccgt cgcccatgcg
     2101 cggattcccg cggtgctccg cgaactcgtt ccgacggcca gtgcggcccg gatcggcggt
     2161 ggccgttgac ggcgagcacg ccaacggcgt acttgcggga aaccagccga gccaggccat
     2221 gcgtctccgg atccttcttc tcccgatcct cctcgtcccc ctggtggtcc gtgcccagga
     2281 ccgcccgctc cgcgtctaca tctccgtgga catggaaggg atcggcggca tcggcacctc
     2341 tgcgatgacg tcggcctcgg gcaaggacta cgcgctcggg cgggagctca tgaccgccga
     2401 ggtgaacacg gtcgtcgagg cggttttcgg gcacggcgag gccgaggtcc tggtcaacga
     2461 ctcccacggc gacatgcaga acctgctgca cgagcggctc gacccgcgcg cgcagtacat
     2521 ccagggcaac atcaagccgc tgggcatggt gcaggggctg gaccgtgcgt tcgacgcggc
     2581 ggtcttcctc gggtaccacg cccgggcggg cactcccgac gccttcctgg cgcacacggg
     2641 ttccggatcg gtgaagggcg tctggatcga cggggtcgag gtcggggagg gcggcatgaa
     2701 cgctctcttc gccgcgtcgc acggggttcc ggtgatcgtt gcgagcggcg accgggcgtt
     2761 cgcggcgcag ttcgcggagc gcgtgccggg ggccatgacg gtcgtgacga aggaggccgt
     2821 cggttcgatg gtcgcgaggc tggaccatcc cgaggtggtc cgggcccgcc tccgggaggc
     2881 cacctcccgc gcgctggcgg ggctcgccgc cctgccggag ccgcaggatc ccggacaggt
     2941 gaccgtgcgc atgcgcttcg ccacgaccac ccgacccgac atcgcgatgg cggtgccggg
     3001 catgcggcgc atcgacggct acaccgtgga attcgacgcg ccggacatgt cggccgccta
     3061 cccgatcgtc cgccttctgt acaagtacgt ttcctggtga gcgggtaggc gattcactca
     3121 caggacgacg cggcccccgc acgacgcgga cctgcatcgg atgcgctacg ttttcgcatc
     3181 cgaacccccg gcccgcatgc gcgacctgag acccgttccc gccttcgtgg cgggcgccgg
     3241 catgatcgcc ggctcgctgc tcacgatccg gcactacttc gaggccaact tcccggcgac
     3301 gatcttcgag ggtgccttct gcgacatcag cgccttcatc aactgcgatt cgtcggccta
     3361 ctccgggatc gcggccgtac agggcgttcc catcggaacg ttcgggatga tgctcggcgc
     3421 gctggtcgtc ctggcctccc tgttcgcgtc ctggcgcctc gcgaagacag tccggttcct
     3481 cgcggccctg aacgtcgcgg gcgtcgtggc gctgctcgcc tggtcggtcg tcgggctggg
     3541 cagcctgtgc ctgctctgcg cgatctacta cgccttctcg ttcctcgcgc tgtgggccgc
     3601 atggccgggg ccgcccggcg cgaaccgctt cgcgccgaat ccaagggtcc tcgccgtcgc
     3661 cggggcggcg acgctcgccg tcgccctggg aatgtcggcc taccacgagg cgcgggtcca
     3721 ggcccggtcg ggcggaaacg cggcgagggt cgtcgcccag ttccggagcc ttcccgtcgt
     3781 ggatccgccg tcggtgctgt cgccgaactg gatcgtgcgc tccacggaac gcttcgagga
     3841 cgccccgctc cgcatcgtgg cctgggagga tttcctgtgc tcggactgcc tgtacctggc
     3901 ccgtcagatc gagcgtctga aggaggagta cggggcgcgc atgaatctcg tgttccagca
     3961 cttcccgctg gacgccgcct gcaacggagt ggtcgagaag gacaagcatc ccggcgcctg
     4021 cgagctgtcc ctcatggcgg cctacgcgcc ggagcgtttc gacgagatct acgaggcggt
     4081 ctatgcggac cagcgcgcgg caaaggatcc cgggtggagg cgcgctctcg cggcgcgcct
     4141 gggcgtggag gccgccgccg gcgactcggc ggcgcgggcg gtcgtggcgg cttccatcgc
     4201 gacgggccgc gagtacgag
//
LOCUS       DBNHQZ010000366         5070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2006942, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000366 DBNHQZ010000000
VERSION     DBNHQZ010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2006942"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..4097)
                     /locus_tag="AC16YU_09860"
     CDS             complement(75..4097)
                     /locus_tag="AC16YU_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HIR6065573.1"
                     /translation="MPENRKPVAASRGLRLALPLLLAAAAPASAQPDVRSSLPSVRGS
                     DAPSVRFESYGLTDGLAQGSVYRMIQDRHGFIWFGTQGGLHRYDGQEFKVYSPVAFDT
                     TSIAEDWIWNITEAGDGAIWTAGQAGSVSRLDPATGRFDNYLPDPADSTKLPGGSAFD
                     VLEARDGTIWVATGRGLARTDPSEPGVFTSMIHVHGDSTTIPGSVHFLHEDADGMIWG
                     GTNNGIIRVDPASGGFTSFWKEGDTHGGTADPQTYFEMLPDADDERVLWIGTGRGLAR
                     FDTRTGSLERYLAFPQDPPGLRRNAHFSVSADPADPEILWVASQGAGLLRFDRAARTF
                     TQYTSDPEDPNSLPDDLVETVFADRSGMMWVGTNRMGLAKFNPGSVRIGHVGHDADDP
                     DALAMGGNLWALMSDSRGRLWAGQSDALGRNLVAVYDPGTGRVDHLRPAPEDPSSIFP
                     GTIASILEDAEGRVWVGSSGLASCDPATLRCRQYPPSVEDSTRLGPGTVQALYQSPAD
                     PGSIWVGSARSGLQKLDIASGRVTRFSVGDPDDGKGPASVAHIIQDAEGTYWLGSWGR
                     GLVRFDEGTGAFRAFSYDPRDTTTVAFDQIEAVVQRPGEPGVLWLGTQAGIDRFDIAT
                     GTARHFGTDKGIADGKVYGMLLDDEGTLWVSTNKGISSFDPETGKYRNYGLDDGLRAL
                     EFQQNGFARGRDGMLYFADVAGITAFHPSQLSTNPVAPELAFTALRVAGHAVAPEPGG
                     ILEKTIAVTESITLSYAQNEFAVDFVALHFSDPARNRFSYRLAGLSDEWVDAGFQRTA
                     AYGNLPPGEYTLQVRAANPDGVWNEEGISLGVTVLPPWWRTTWAYVLFAALLAGLVFG
                     VDRLQRYRLLRTERERAAIQEAELRAETAEAEAKALQAENDRKKNIERLSEIGAEITS
                     SLDVETIFGRLYEHVNELTDAPIFGVGIYHEDRQVIDYRMAIENGKRYAPYTRDTSEK
                     DQFPVWCIEHRKPVFINDVESEYSKYIERYEHETGTLEDGTTSQSPLSLIYLPLVTQD
                     RVLGVITVQSFRKNAYSEDDLNILRTMAAYASVAIDNANAYRQLNGTVAELQQTQQQL
                     VQQEKMASLGQLTAGIAHEIKNPLNFVNNFADLNAEMATELRELLAGADSAELASKRA
                     ELDDLVATLQTNARQIAKHGRRADEIVKGMMQHSRPGDAERYPVAVNDFVDEYLNVAW
                     HGHKAQHPDLDVQVVREYAPDVGNATLVPQDMGRVVVNLVGNAFDILRGREDARLRVS
                     TARRGALVEIRVADNGPGVPDSIRSRIFEPFFTTKPSGQGTGLGLSMAHDIVTQGHGG
                     TMRVENNAEGGATFVVSLPA"
     gene            complement(4293..>5070)
                     /locus_tag="AC16YU_09865"
     CDS             complement(4293..>5070)
                     /locus_tag="AC16YU_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR6065574.1"
                     /translation="DENELFRSGSLGPAELAEIRERHRQWLDGASGGEDGRPVLRQAG
                     LSGPDQDIIVLATPEMAGLARWIIALVAAGGLAAALSTASGLLLVISSSVAHDLYFRI
                     VNPAASEGQRLLVGRTVIGFAVVVAGLLGIYPPGFVSQVVAFAFGLAAASFFPAIVLG
                     IFSRRVGTVPAMLGMIVGLGFTATYIVGSVYGGWTSWAFGIGPQGIGVVGMALNFAVT
                     LGLTPFFPAPDRSVREMILSIREPEGAGPGLDIDHSVEAD"
BASE COUNT          778 a         1757 c         1744 g          791 t
ORIGIN      
        1 cacggacgag gagtgggagg cggcccggta gccgggcgcc ccgcgcgtgt cggctacgac
       61 cggcaaggct cccctcacgc cggcagcgac acgacgaacg tggctccgcc ctccgcgttg
      121 ttctccaccc gcatcgtgcc gccgtgtccc tgcgtcacga tgtcgtgggc catgctcagg
      181 cccaggccgg tgccctgccc ggacggcttc gtcgtgaaga acggctcgaa gatccgggac
      241 cggatcgaat cgggtacgcc gggtccgttg tcagccacgc ggatctcgac cagggcgccg
      301 cgacgcgccg tggacacgcg gaggcgcgca tcctccctcc cgcggaggat gtcgaacgcg
      361 ttgccgacca ggttcacgac cacgcgcccc atgtcctgcg ggacgagggt cgcgttgccc
      421 acgtcgggcg cgtactcccg caccacctgg acgtcgagat ccgggtgctg ggccttgtgc
      481 ccgtgccagg cgacgttcag gtactcgtcc acgaagtcgt tgacggcgac cgggtagcgc
      541 tcggcgtcgc ccgggcgcga gtgctgcatc atgcccttca cgatctcgtc cgcgcgccgg
      601 ccgtgcttcg cgatctggcg ggcgttggtc tgcagcgtgg cgaccaggtc gtcgagctcc
      661 gcgcgctttg aggcgagctc ggccgagtcg gctccggcca ggagctcgcg cagctcggtg
      721 gccatctcgg cgttcaggtc ggcgaaattg ttcacgaagt tgagcgggtt cttgatctcg
      781 tgcgcgatgc ccgccgtgag ctgccccagc gacgccatct tctcctgctg cacgagctgc
      841 tgctgggtct gctggagctc ggcgaccgtc ccgttgagct ggcggtacgc gttcgcgttg
      901 tcgatcgcca cggacgcgta ggccgccatc gtccgcagga tgttcaggtc gtcttccgag
      961 taggcgttct tccggaagct ctgcacggtg atgacgccca ggacgcggtc ctgggtgacg
     1021 agcggcaggt agatgagcga gagcggcgac tgcgatgtcg tgccgtcctc gagcgtgccc
     1081 gtctcgtgct cgtaacgctc gatgtacttc gagtactccg actcgacgtc gttgatgaag
     1141 acgggcttcc ggtgctcgat gcaccacacg gggaactggt ccttctccga cgtgtcgcgc
     1201 gtgtacgggg cgtaccgctt gccgttctcg atcgccatcc ggtagtcgat cacctgccga
     1261 tcctcgtggt agatgccgac gccgaagatc ggcgcgtccg tgagctcgtt cacgtgctcg
     1321 tagagcctgc cgaagatggt ctccacgtcg agcgacgacg tgatctcggc gccgatctcg
     1381 gacagccgct cgatgttctt cttgcggtcg ttctcggcct ggagcgcctt ggcctcggcc
     1441 tcggcggtct cggcacgcag ctcggcctcc tggatggccg cgcgctcccg ctcggtacgc
     1501 aggaggcggt agcgctgcag gcggtccacc ccgaagacga ggccggcgag aagcgcggcg
     1561 aacagcacgt acgcccacgt ggtgcgccac cacgggggca ggaccgtaac gccgagcgag
     1621 atgccttcct cgttccacac gccgtccggg ttggcggcgc gcacctggag ggtgtactcg
     1681 ccgggcggca ggttgccgta ggccgccgtg cgctggaatc ccgcatcgac ccactcgtcc
     1741 gagaggccgg ccagccggta cgagaagcgg tttctggcag ggtcggagaa gtgcagcgcc
     1801 acgaaatcga ccgcgaactc gttctgggcg taggacagcg tgatcgactc cgtcacggcg
     1861 atcgtcttct ccagtatgcc gccgggctcc ggggcgaccg cgtgtcccgc gacgcgcagc
     1921 gccgtgaacg ccagctccgg cgcgacgggg ttcgtgctca gctgcgacgg atggaaggcc
     1981 gtgatgccgg ccacgtcggc gaagtagagc atgccgtcgc ggcccctggc aaagccgttc
     2041 tgctggaact cgagcgcccg gagtccgtcg tccaggccgt agttgcggta cttgccggtc
     2101 tcgggatcga acgacgagat ccccttgttc gtgctcaccc acagcgtgcc ctcgtcgtcg
     2161 aggagcatgc cgtagacctt tccgtcggcg attcccttgt cggtcccgaa atgccgggcc
     2221 gtgccggtcg cgatgtcgaa ccggtcgatg ccggcctggg tgccgagcca gagcacgccg
     2281 ggttcaccgg gccgctggac gaccgcctcg atctggtcga aggcgacggt ggtcgtgtcg
     2341 cgcgggtcgt aggaaaaggc ccggaaggcc ccggttcctt cgtcgaagcg gacgaggccc
     2401 ctgccccagc ttcccaacca gtaggtgccc tccgcgtcct ggatgatgtg cgcgacggag
     2461 gcggggcctt ttccgtcgtc cgggtcgccc acggagaacc gcgtcaccct tccgctcgca
     2521 atgtccagct tctggaggcc gctcctggca cttcccaccc agatcgagcc cgggtcggcg
     2581 ggagactggt acagcgcctg gacagtgccc ggccccagcc gggtcgaatc ctcgaccgac
     2641 ggcgggtact gccggcaccg gagcgtggcg gggtcacacg aggcgagtcc gctcgatccg
     2701 acccacacgc gcccctcggc atcctcgagg atggacgcga tcgtgcccgg gaagatcgac
     2761 gaaggatcct ccggagccgg tcgcaggtgg tcgacgcggc cggtccccgg gtcgtagacc
     2821 gccacgagat tgcgcccgag cgcgtcggac tgcccggccc agagtcggcc cctcgagtcg
     2881 gacatgagcg cccacaggtt gccccccatc gccagggcgt cgggatcgtc cgcgtcatgt
     2941 cccacatggc cgatgcggac cgagcccgga ttgaatttcg cgagccccat cctgttcgtg
     3001 cccacccaca tcatcccgct ccggtccgcg aaaaccgtct cgaccaggtc gtcgggaagg
     3061 gagttcggat cctcggggtc ggaagtgtac tgcgtgaacg tgcgtgcggc gcgatcgaag
     3121 cggagcagcc cggcgccctg cgaggccacc cacaggattt cggggtccgc aggatccgcc
     3181 gacaccgaaa aatgcgcgtt ccgcctgagc ccgggtggat cctgggggaa tgccaggtac
     3241 cgctcgagtg agcccgttcg cgtgtcgaac cgggcgagac cccgtccggt gccgatccac
     3301 agcacccgct cgtcgtccgc gtccggaagc atctcgaagt acgtttgcgg gtcggccgtc
     3361 ccgccgtggg tgtcgccttc cttccagaac gaggtgaacc cgcccgacgc agggtcgacc
     3421 cggatgatgc cgttgttcgt gccgccccag atcatgccgt ccgcgtcctc gtgcaggaaa
     3481 tgcaccgaac ccggaatcgt ggtggagtct ccgtgcacgt gaatcatcga ggtgaacacg
     3541 ccgggctcgg acggatccgt ccgcgcgagg ccacggcccg tcgccaccca gatcgtgccg
     3601 tcccgcgcct cgagcacgtc gaaggcggac ccgcccggaa gcttcgtcga gtcggctgga
     3661 tccgggaggt aattgtcgaa acgcccggtc gccgggtcca gccggctcac cgagcccgcc
     3721 tggccggcgg tccagatggc gccgtcaccc gcttcggtga tgttccagat ccagtcctcg
     3781 gcgatggacg tggtatcgaa ggccacaggg gaatagacct tgaattcctg cccgtcgtac
     3841 cggtgcaggc cgccctgcgt cccgaaccag atgaatccgt ggcggtcctg gatcatacgg
     3901 tagacggagc cctgcgccag cccgtcggtc aggccgtagc tctcgaaacg gaccgacggg
     3961 gcgtcgctgc cccgcactga cggcagcgag gagcgcacgt cgggctgggc agaagccggg
     4021 gcggcggcgg ccagcagcag cggaagggcg aggcgcaatc cacgggaagc cgcgaccggc
     4081 ttgcggttct caggcatgca cgacaacaag ggtgacctgg gggaagtcgg gatcggcacg
     4141 cggcgcccgg cggtgctctg ggggcaccgg gactccgtcc gaaacggtga aaataccaag
     4201 aaagtaggtt aggtgcccgg gagcggccag tcaatagcga cggccgacaa tccgggaccg
     4261 cgcgcgtggc cgcaccgagc cacgccggcc gctcagtccg cttccacgct gtggtcgata
     4321 tccagccccg gtcccgcgcc ctcgggctca cggatcgaga ggatcatctc ccggaccgac
     4381 cggtccggcg ccgggaagaa cggcgtgaga cccagcgtga ccgcgaagtt cagggccatc
     4441 ccgacgaccc cgatgccctg cggtccgatg ccgaacgccc acgaggtcca tccgccgtac
     4501 acgctgccca cgatgtaggt ggcggtgaat ccgaggccca cgatcatccc gagcattgcc
     4561 ggcaccgttc cgacgcgccg cgagaagatg cccagcacga tggccgggaa gaagctcgcg
     4621 gccgccaggc cgaacgcgaa cgcgaccacc tggctcacga agcccggcgg gtagatgccc
     4681 aggaggccgg ccaccacgac cgcgaacccg atgaccgtgc ggcccaccag caggcgctgt
     4741 ccctcggacg cggccggatt cacgatgcgg aaatacaggt cgtgggcgac gctcgacgag
     4801 atcaccagca ggagtccgct cgccgtggac agggccgcgg ccaggcctcc cgccgccacc
     4861 agggcgatga tccaccgggc caggcccgcc atctcggggg tggcgagcac gatgatgtcc
     4921 tggtccgggc ccgagagccc ggcctggcgg agcaccgggc gcccgtcctc cccgccggaa
     4981 gccccgtcga gccactgcct gtgccgctcg cgaatttccg cgagttcggc gggacccagg
     5041 ctgccgctgc ggaacagctc gttttcgtcc
//
LOCUS       DBNHQZ010000367         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1600382, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000367 DBNHQZ010000000
VERSION     DBNHQZ010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1600382"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1233
                     /locus_tag="AC16YU_09870"
     CDS             250..1233
                     /locus_tag="AC16YU_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6065575.1"
                     /translation="MGNRYEPEDSTTEARVAEALRFGLLQGGASPAGPDEARLFEAVR
                     MLRDDYVASAAVPDADASSRMLAGIRAGMERTRRAPSPRARLADVARWILLRPVGVAV
                     AAVVVVAIAVLVLRLPGGRIVAESGSQTLVYDAPDGSVVTLRPHSRLIATGDDDVRSY
                     TLVGEAHFAVARDVARTFTVEAGEGRVQVLGTVFTVGSWGRTVRVYVDEGSVRFEGLR
                     TGSSLDLAAGVGSELVDGNPVVTTRLDRAAALDWMEGALVLDSQALGDVIAEIRQHFD
                     VRIDFPQELESERLTGRILLPDVERALDDLGIVVGGEFVRTGKDAFRFERD"
     gene            1268..>2452
                     /locus_tag="AC16YU_09875"
     CDS             1268..>2452
                     /locus_tag="AC16YU_09875"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6065576.1"
                     /translation="MPDRDVHPRVMPREAAPARWPAGWTVVVAMLLGLLVHAPARAQG
                     VFVFEQAPLSDVIREIEERTSYRFLYRDALISGRRVTLDSDQADVFVEFSKAVDALGL
                     DVMIDDSRGQVLLFARRTRRAPAAVVTGSVFDAESGVRLPFATVSWHSATGLRGAVAD
                     DAGNFTLTLPPGTGPVDLTASFLGYRAHVVRIDSEKPPLELSIRLRPEPILASEVLVR
                     GSMLHTDLDTAWHHLMQPGSLAPLGESGVLRSLQTLPSVAITSAVSEGLTVRGSRSDG
                     FQVLLDGMPVYGESHLFGMFDAFNDDAIQAVGFFYGITPASYQAPPGGTLSFLTRSGS
                     RSRFGGSASVSNTSVRGTAEGPLFGGSGSWLVSGRHSIISAVNWMGNENLVAHGLDVD
                     REH"
BASE COUNT          333 a          818 c          883 g          418 t
ORIGIN      
        1 ctgatccggg cccacaacct gggcggcttc ggtccgggct tccaccatgg gatgggcatg
       61 gggccgggcg gcttcatgaa cggagacggc acgcagacgc agacccagca gcagggaggc
      121 tacggcatgc ccggaggctc cggcagcggc aacggaatga atggtgggta aacggcaagg
      181 gatccggcgg gaccggccga gtgccggtcc tgccggctgg cccttacctt ccaagccaat
      241 cgacgtcaca tgggcaaccg ttacgaacct gaagacagca ctacggaggc gcgggtcgca
      301 gaggccctgc gcttcggtct gttgcagggg ggcgcttcgc cggccgggcc cgacgaggcg
      361 cgccttttcg aggccgtgcg gatgctcagg gacgattacg tggcgtcggc cgccgtgccc
      421 gacgcggacg ccagttcccg gatgctggcc gggattcgcg cgggcatgga acggacgcgg
      481 cgggcgccgt cgccgcgtgc ccgccttgcg gacgtggcga ggtggatcct gcttcgcccg
      541 gtcggggtgg ccgtggccgc cgtcgtggtt gtcgcgatcg ccgtactcgt tctgcggctg
      601 cccggcggga ggatcgtggc cgagagcggt tcgcaaacgc tcgtctacga cgctccggac
      661 ggctcggtcg tcacgctgcg cccgcattcg cggctgatcg cgacgggcga tgacgatgtg
      721 cggagctaca cgctcgtcgg cgaggcgcac tttgcggtgg cgagggacgt ggcccgtacg
      781 ttcaccgtcg aggcgggcga aggacgcgtg caggtcctgg gcacggtgtt cacggtgggg
      841 tcctggggcc ggacggtgag ggtatacgta gacgagggca gcgtccgctt cgagggtctc
      901 cgaacgggat cgtcgctgga cctcgcggcg ggcgtcggat cggagctcgt ggacggaaat
      961 ccggtcgtca ccacccgcct cgaccgtgcc gcggccctcg actggatgga gggcgccctg
     1021 gtgctcgact cgcaggccct cggggacgtg atcgcggaga tcaggcagca tttcgacgtg
     1081 cggatcgatt tcccgcagga actcgagtcc gagcgactca cgggccggat cctcttgccc
     1141 gatgtggagc gggcgctcga cgatctcggg atcgttgtcg gcggtgaatt cgttcggacc
     1201 ggtaaggacg ccttccgctt cgagcgcgac taggcggatc gcctgcccgt tacccgaatc
     1261 agccgccatg cccgacaggg acgttcaccc gcgcgtgatg ccgcgtgagg cagcccctgc
     1321 gcgatggccc gcaggctgga ccgtcgtcgt cgcgatgctt ctgggactcc tggtgcacgc
     1381 ccccgcgcgg gctcagggag tcttcgtgtt cgagcaggcg cccctctcgg acgtgatccg
     1441 cgagatcgag gagcggacgt cctaccggtt cctgtaccgt gacgcgctca tctccggccg
     1501 tcgcgtgacg ctcgattcgg accaggcgga cgtcttcgtc gagttcagta aggccgtgga
     1561 cgcgctgggc ctggacgtga tgatcgacga ctcgagaggc caggtcctgc ttttcgcgcg
     1621 gcgtacccgg cgcgcccccg ccgcggtcgt gacgggctcc gtgttcgacg cggaatccgg
     1681 cgtccgcctt ccgttcgcga cggtctcgtg gcattccgct accgggctcc gcggggcggt
     1741 cgcggacgac gccggcaatt tcaccctgac gctgcccccc gggacggggc cggtcgacct
     1801 gaccgcctcg ttcctcgggt accgtgcgca cgtggtccgg atcgactcgg agaagccgcc
     1861 cctcgagctc tcgatacgcc tgcggcccga gccgatcctg gcttccgagg tgctcgtccg
     1921 cggaagcatg ctgcacacgg acctggacac cgcgtggcac cacctgatgc agccgggctc
     1981 actcgcgccg cttggagaga gcggggtact tcgctccctg cagaccctgc cgtccgtggc
     2041 catcacctcg gccgtcagcg aggggctcac cgtgcgcggg agccgatccg acggtttcca
     2101 ggtgctcctg gacggcatgc ctgtctacgg agagtcgcat ctcttcggca tgttcgacgc
     2161 gttcaacgac gatgccatcc aggccgtggg gtttttctac gggatcaccc cggcctcgta
     2221 ccaggcgcct ccgggaggca ccctgtcctt cctcacgcgc tcgggctccc gctcccggtt
     2281 cggcggttcg gcttcggtct ccaacacgtc ggtccggggc acggccgaag gacccctgtt
     2341 cggcggctcg gggagctggc tggtctcggg tcggcactcg atcatctcgg ccgtgaactg
     2401 gatgggcaac gagaacctcg tcgctcatgg cctggacgtg gaccgggagc at
//
LOCUS       DBNHQZ010000368        20673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1600754, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000368 DBNHQZ010000000
VERSION     DBNHQZ010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 20673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20673
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1600754"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC16YU_09880"
     CDS             complement(<1..467)
                     /locus_tag="AC16YU_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065577.1"
                     /translation="MRHARLSVIVALAFLTAPAALAQGIYTERGFGVELGYQSGDNAS
                     SFGGAVGYAVNRIFEAGLGIERESYDGSDGNRTYITPRVRGYFARQSEAMPVTIFGEG
                     HYHFVSVSEAPDDWSESGWAVGLGAAHSLAASEALAITPIVSVMYSGYTIEFMG"
     gene            762..1133
                     /locus_tag="AC16YU_09885"
     CDS             762..1133
                     /locus_tag="AC16YU_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR6065578.1"
                     /translation="MLSDFLPLFMMLVIAGGVALMLLKSAELVGPKRPNIIKQKAYES
                     GMDPIGTARDRYSVKFYLVAMIFIVFDVEVVFLYPWAASFSEFLDSGAALGVMGVVVL
                     FIVILAIGLLYDIRKGGLEFD"
     gene            1176..1769
                     /locus_tag="AC16YU_09890"
     CDS             1176..1769
                     /locus_tag="AC16YU_09890"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR6065579.1"
                     /translation="MAPDPVTGGEGFLTTRVDAVLNWARSNSLMPMPMGLACCAIEMM
                     AMAAPKYDVARFGSEAFRFSPRQADLMIVAGWVSYKMAHTIRRIWDQMADPKWCIAMG
                     ACASSGGMHRCYGVVQGCDNFLPVDVYISGCPPRPEAVIHALMDIQEKVRNQYSVAAD
                     SASGEVQPEPAALRPRVLTPGGDRVISPVALYDQENL"
     gene            1824..2495
                     /locus_tag="AC16YU_09895"
     CDS             1824..2495
                     /locus_tag="AC16YU_09895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR6065580.1"
                     /translation="MTQGRTLAFHFTPVDGPRGTGDDKNPHAKDTTFVPEVVDALRQR
                     FGDAVQEVILHANEHTVYVDRASIVEICRYLKEDQQFEYLADIGGIDLFTDENRFEVF
                     YNLVSMARGKRLRLKIRVPEEEPVVASLVPVYPAANWNERECWDMFGIRFDGHPDLRR
                     MFMPEDFEYHPLRKEFPLLGVPGSLPLPPQAPEAGLTLDPFAAAHGSRPVKSFQEAPS
                     DLAKK"
     gene            2509..3828
                     /gene="nuoD"
                     /locus_tag="AC16YU_09900"
     CDS             2509..3828
                     /gene="nuoD"
                     /locus_tag="AC16YU_09900"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR6065581.1"
                     /translation="MSKISTENFGETLKFWPRHHEALYRRLESKHSWLEERHGPRQKG
                     DSDPLEHEMVLNIGPQHPATHGVLRCAVKLDGETIERCVIDIGYLHRGLEKVAENKTY
                     QEFMPYTDRMDYLAPYANNVGWCLAVEKAAGIEVPERAQWIRMMMSELSRISSHLIWM
                     GVGLMDAGAVSVFLWTFKYREDLYSIFDHVAGARLTVSHSRIGGVASDVSDEAIAMIQ
                     AFVDDFPAAVLGWEKLLNRNRIWIDRNVGIGVLTREDALALGLTGPNLRGSGIPHDIR
                     RFEPYLKYDEVDFNIPIREEGDAFSRYYVRLDEMKESLRIVQQCLEKLETVRGPLRTD
                     DAKRSYPSKDEVYYSMEGLIHDFMYTDTGVCPPKGTWVYHAIEAPKGELGFYIQSDGT
                     GHPFRARINAPSFTNIQGLEALLEGSMMGDMVILIGTVDPVLGEADK"
     gene            3836..4510
                     /locus_tag="AC16YU_09905"
     CDS             3836..4510
                     /locus_tag="AC16YU_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR6065582.1"
                     /translation="MADFIKHPVVPLPDRDPEPVIPADRLLFTDDEKRSIAEWISRYP
                     SADGAIMRALWLAQEKFGFLPPEVIRLTADQVGIPYAQAYGVATFYTQYYKKEMGRHV
                     LDVCTCFSCQLCGGYDMLHHLERKLGIGKGETTPDGAFTIQEVECLGACGSAPVLQVT
                     NGPYVFNLTEERLDALVDALREDRIPEFTSVTLPQDQDELGGNRRSDVPDTVGSLTPP
                     VSSTIR"
     gene            4542..5873
                     /gene="nuoF"
                     /locus_tag="AC16YU_09910"
     CDS             4542..5873
                     /gene="nuoF"
                     /locus_tag="AC16YU_09910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR6065583.1"
                     /translation="MEASNGSKAGDWRSYRRVLLPPVRDLHRLDVYEANGGYGALRSV
                     LTGAKWDPKSVTEEVKKSGLKGRGGAGFPTGLKWTFMPPVDPAIPRFLCCNGDESEPG
                     TFKDRQLMEYNPHLVFEGMLIAAYAMSVKTSYLYVRGEYADWISHMERELEKLYAKGY
                     VGKNVMGSDFSTDIVIHKGAGAYICGEESSLMESLEGKRAYPRIKPPFPAQRGIWNYP
                     TTINNVETLACVPLIIERGGDWYAKIGAPTHTGPVLYGVSGHVNRPGVYEYPTGMLIT
                     DLIQEVAGGMRGGKKLKAVVPGGSSTPVLRADQIDGVTMDPDSLRTVGSMMGTAGMLV
                     MDQDTDMVSFLRRITHFYHHESCGQCTPCREGTGWLENLVTRIDEGEGKLGDLDLLVD
                     ICSQMEGRTVCALADAAAWPVRYTIERFRSEFEAKCRPSLVPASGIPVHQD"
     gene            5905..6444
                     /locus_tag="AC16YU_09915"
     CDS             5905..6444
                     /locus_tag="AC16YU_09915"
                     /inference="COORDINATES: protein motif:HMM:NF027592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HIR6065584.1"
                     /translation="MTRLLYALFALAVLLGGCRADAPGTDAGTAEEAAETSPGWDAVG
                     EALVAEGAVLTQDLAADPEPFVGKEVTIAGVVAEVCQSSGCWLTLAAGPDRSPIRVDV
                     PRDSAGVYVYTFPKDISGRTVVIAGMLRAGDEAEHEHGMTEDAHATEVEGHDEAEARA
                     LALDLAIVARGALIERIGP"
     gene            6470..8188
                     /locus_tag="AC16YU_09920"
     CDS             6470..8188
                     /locus_tag="AC16YU_09920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR6065585.1"
                     /translation="MPTVTIDGAAYEFEGRPRLLQFCLDHGLELPHFCYHPAMSAPAN
                     CRQCLVEVGMPARNRETGQPQLDDAGNPVISWMPKLQTSCSLDMADGMVVRSHRSSDL
                     VARAQKDNLELLLINHPLDCPICDQAGHCPLQIQAYKYGPEGSRFEFEKTHKTKQLEL
                     GPRVVLDGERCINCTRCVRFTDEISKSHQLTIIERGVRNYPMTPPGTIFDEPYSMNVI
                     DICPVGALTSVDWRFQARIWEMSKTASITTGNAKGSNCWYWVKDNRVMKVTPRDNESV
                     NEYWLPDEDRLDTARFNEDRPAGPSVDGSPATWDDAYRAAAALLRDVDPSKVAFLGSA
                     YATVEDNYLLARLASAVGADAPRYIPHVEPGHGDGWLRTDDRTPNAQGCERLGIHPVD
                     AATLKARLAGGAIDVLYVLEDDPVGSGLCDEADLSGVSVVLHPYNTTNRTMGAARVTL
                     PAATVVETVGTFVSEDGHAQRVRPAKAIRGINRTLMMEVGKSRHDLHGTPFDRWFSEA
                     NLVDCQPGWVSLPGVAAAAGHPMDYHGPRAIMDEIAGSIDAFASATYEAMGLAGVRLQ
                     EAPTPA"
     gene            8262..9293
                     /gene="nuoH"
                     /locus_tag="AC16YU_09925"
     CDS             8262..9293
                     /gene="nuoH"
                     /locus_tag="AC16YU_09925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR6065586.1"
                     /translation="MDLAWYWTALIAFLVANFFLVSAAVLVYAERKVSGYMQNRPGPN
                     RVGPFGLLQPFADVLKLILKENIVPTSATAFVHSLAPSIMILIAFTGGALIPFARGVV
                     IADMEVSVLILLALTSISVYGVTLAGWSSNSKYSLLGGLRSSAQMISYELSMGLAVVS
                     VILLAGSLNLSNIVESQAGGYGILGWNIFRNPIGAVIFIVTAFAECNRTPFDLPEAEQ
                     ELVGGYHTEYSGMKFGMFFLAEYVNFFMASFVISTLFFGGYLLPFESLILGAFPALEG
                     SVLLGVLQVLSLLVKAGFFAFTFIWVRWTLPRFKYQQLMKLGWKVLLPLALVNAILVA
                     TGIAIVRGF"
     gene            9427..10101
                     /gene="rpe"
                     /locus_tag="AC16YU_09930"
     CDS             9427..10101
                     /gene="rpe"
                     /locus_tag="AC16YU_09930"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6065587.1"
                     /translation="MPILAPSILAADFANLESHCREAFEAGAEWIHVDVMDGRFVPNI
                     TIGPPVVHSLRPFADDTGALLDVHLMIEEPGRYVEAFAAAGADRITVHVEACTHLHRT
                     LQSIRERGVRAGVTLNPGTPVQSVEPVLGLVDLALVMTVNPGFGGQAFIPESLGRIRA
                     VRSMLDALGSRAHVQVDGGVTPENVATVIEAGADVVVAGSAVFRGRGTVAENVQSFRR
                     AWSLST"
     gene            10083..10949
                     /locus_tag="AC16YU_09935"
     CDS             10083..10949
                     /locus_tag="AC16YU_09935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6065588.1"
                     /translation="MVSFDMNEVEGMTCVVTGATSGIGRVAAAEFARCGARVVIVGRS
                     RERGRHTLVEIMRDTGNTQVDLILADLSSQADVRRLAADITARYPQIDVLVNNAGAIF
                     RRREESVDGIEMTLALNHLGYFLLTNLLLDTLRSSAPCRIVNVASSAHRRARLDLDDL
                     QSRRRYRAWRAYANSKLCNLLFTYELARRLEGTGITVNALHPGTVATRFGSNNSGPMR
                     RMMVFGRPFLRTPERGAETIAHLALAPELASSTGGYFVDERPRRSSRASHDAAVAARL
                     WDLSAELTGIGR"
     gene            complement(10953..12098)
                     /locus_tag="AC16YU_09940"
     CDS             complement(10953..12098)
                     /locus_tag="AC16YU_09940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6065589.1"
                     /translation="MRRPSFHILALLLLVGCNRNVQSQEQALPPQRVPAEVLLGQDDI
                     GRSRQNAITTAVQVASPAVVSVNAIKRTVYSDPFNDPIFDFFFGGQRSRMRERRVQSM
                     GSGFVISPDGYIVTNDHVAAGADEITVAFTNGRTHTASLVGTDKASDLTLLKVESDEP
                     LPYLAFSTDPSPIVGEWCIAMGNPFGLFEASDPTVTVGVVSAIGRDLSPKEGHMYQDM
                     IQTDAAINSGNSGGPLLNAAGEVIGVNTAIVTPAGGSVGIGFAVPAERALKVVGELRD
                     KGFVDRSYYTGLRVREVTARIAQALRLPEARGVFIEDVEYGSPAEDAGFGPYDVIVAV
                     EGEAVANRNDVVQRLYDFRPGDEVRFTVIRGGEQVELSMTLGSGNSR"
     gene            12205..13446
                     /locus_tag="AC16YU_09945"
     CDS             12205..13446
                     /locus_tag="AC16YU_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HIR6065590.1"
                     /translation="MTCSAVPEPRIDAARLVRDLERLARVGARTDGGVTRLAFSAEDR
                     AGRDVVQKLLAELGVASRIDRVGNLVGIRAGTEADAPVVLTGSHTDTVEAGGRFDGAL
                     GVLAGVAVLRALDAAGALTRRPVGVVSFVNEEGVRFMPDMMGSLFVRGDLSLADIHAA
                     VGTDGATIGDELARHGLSGDDDFSALPVSAFLELHIEQGPVLDAAEEPVGVVTGVQGL
                     RWLRVRVSGASNHAGTTPMRGRRDAGLVAARIAAGVRELAMTMAPLRATCGRLVLEPN
                     VVNVIPASAELTVDLRHPDADGLSAAEAAVRALVAREAAGETVHAAVEPLAHAPPVTF
                     DSRIVSAVERAAAALGLPCRRLLSGAGHDAQILSRAWPSAMIFVRSRNGISHSPAEWS
                     SPDDLVAGAELLLGSVLELAG"
     gene            complement(13472..14305)
                     /locus_tag="AC16YU_09950"
     CDS             complement(13472..14305)
                     /locus_tag="AC16YU_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6065591.1"
                     /translation="MEWMDVAVLATAGLAGGFVGGLVGVGGGVIFAPVLFFYLQAQGV
                     TDPTLTPLTIGSSLFGTLVTSGVSARAQHLRAAVDWRIATLTGLSSAVVVLAMTWFVT
                     TRPWYDATVFQVVFSAVLVWVGLRMLLARPERGGAAEGRSARGGGGLLLAGMGSVAGA
                     VSAAVGVGGGVVLVPAYHELLHLSMHRSVGTSSASIVFIALIGVLAYVATGWTAPVPP
                     TALGYVDVGAALVLSVAAAGAARLGVTTAHHINTRWLERAFAVLVFLVAARLVLRALG
                     T"
     gene            complement(14311..15954)
                     /locus_tag="AC16YU_09955"
     CDS             complement(14311..15954)
                     /locus_tag="AC16YU_09955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6065592.1"
                     /translation="MRPLYASSTLLLLLVGGSVLWTGSRPPDDDFYAMRKNFELFGSV
                     YEELVGTYVTRIDPERFMRTGMDAMLGTLDPWTDFLDEADNVQIDLRGRRLLGDVGLN
                     LGRRGGRITVLVPDEAASAYRQGIRTGDVVRSVGGQSVDSLSVPDVQELLRGDPGSVV
                     DVEIEREGSGVLVFRLRREAPTARNVSLAERLPAPNDDIAYLKLDRFGQAAGAELAAA
                     YRGIEAAGPVRAVILDLRNNPGGLLSQAIAVSQLFLPLGTLVVSTRGRAPESNRAYHT
                     QGDPVIPDVPVAVLVNGYSASASEIVAGALQDHDRAVVVGSTSFGKGLVQVFRGLPYN
                     TSLKLTMARYYTPSGRGIRKDALENAEAPSREEFFTAAGRTVREGYGVEPEVLVPDAA
                     EGPLEEALEREAAFFLFAGVIVAEQGAELEAALEAGRPLPIDDGTLERFRRWLAERDF
                     EFRTPFERSLSELSADDRTPPVRQAWDALQEAAARERDGAFERERERLVSRLSDEVAG
                     RLMGSTDQVRWSLSDDPVLGGALEVVGSRERYDAALGSR"
     gene            complement(16019..16912)
                     /gene="tatC"
                     /locus_tag="AC16YU_09960"
     CDS             complement(16019..16912)
                     /gene="tatC"
                     /locus_tag="AC16YU_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR6065593.1"
                     /translation="MSPFVRIHDMLMGLRRRKEARAAQLKAAREQGSLVDPEEGMLEM
                     GFLDHLEDLRWSLIKGFSGILVFTIGAIFFRRWIIENLLLAPKSPDFFMYRLFGVEAT
                     EFVLQNRTITGQFFADMGTIIAVGVILGSPVFVYQMWRFVEPGLYPSEKKGMRFAAVF
                     ATFFFAFGISFGYLIVTPLALQFFAGYSISAEISNEFDISRYLSMITTWSFGAGVLFE
                     LPVVIYFLAKLGIATPGRLRGFRKWNLIICLTLGAIFTPPDPISQVIVATPLVLLYEL
                     GIWISSVVTRRRERELAQALA"
     gene            complement(16914..17528)
                     /locus_tag="AC16YU_09965"
     CDS             complement(16914..17528)
                     /locus_tag="AC16YU_09965"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046132 - pyrimidine ribonucleoside
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6065594.1"
                     /translation="MTEDLTDIPTGSAGYAELLDLGRRLYDRALVRREQELITDPRGQ
                     AIGWLLDTRMAMLDGVLFREVGDVLADRVRSRGVNQVAGFGFGSYALVCSVLSSGTGP
                     SLSGGFVREHRKPHGRRRLVEGPIDRARPLVVLDDILNSGRSAARAVGLLRQDGFQVA
                     GLLTLFNFTWSGGRARVEELGLWVDSLLDLNLRDHRPGSSDSSW"
     gene            complement(17605..18912)
                     /locus_tag="AC16YU_09970"
     CDS             complement(17605..18912)
                     /locus_tag="AC16YU_09970"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6065595.1"
                     /translation="MSELQSHDPEVYASIAREVGRQNDGLELIASENFVSRSVLEAMG
                     SPLTNKYAEGLPGKRYYGGCQFVDEVEDLARDRARQLFGAEWVNVQPHSGASANASVY
                     LAFMNPGETFLGLDLAHGGHLTHGSPVNFSGILYRAEHYGVERDGPMAGRIDMDRVRD
                     KAKAVQPRMISIGASAYSRDFDYAAFRSVADEVGAFLWMDMAHTAGLIAADVLNDPLP
                     HCHVVSTTTHKTLRGPRAGMILLGRDYENPFGKVAPKSGRVKMMSELLDAAVFPGMQG
                     GPLMHVIAAKAVSFAEALDPSFRTYAREVVRNAQVMATAFMERGYDLVSGGTDNHLVL
                     IDLRSKGITGKDAEAVLERADITVNKNMVPFDTESPFVTSGIRVGTPAMTTRGFGPDE
                     FSRVVELVDRVLSAPGDESVIASVREEVRMMCRDFQLYDFVVV"
     gene            complement(19009..19347)
                     /locus_tag="AC16YU_09975"
     CDS             complement(19009..19347)
                     /locus_tag="AC16YU_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HIR6065596.1"
                     /translation="MRFLIVVVLALAVGGFLARYNPGESEFREFVRERVEKRLSSDLG
                     TERVGRIVTAFGSDVAGAIAARAAERDDYLLFSIFRVDLGADGREEDEWRFVGVAGSF
                     FELSRPEILQ"
     gene            19644..>20673
                     /gene="argS"
                     /locus_tag="AC16YU_09980"
     CDS             19644..>20673
                     /gene="argS"
                     /locus_tag="AC16YU_09980"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR6065597.1"
                     /translation="MRDYLASRILAALETLGGAPDGFVPEFQQPSNPEHGDLASNAAL
                     QLARHFRKAPRQVAEDLSGALDLDPRRITAVEIAGPGFINFRFGTAWLTDELSRIVEA
                     GERYGRTEGGRGKSAIVEFVSANPTGPLTVGHGRNAVLGDTVANLFDWTGYAVTREYY
                     FNDAGRQMRILGESVRARYLALVDPSRGTREIDGPSGPVTVPADFPEDGYLGEYIGDI
                     ARGLFDAHGADLAGADAEGPFKEAAEKAIFREIEATLVRLGIRMDTYFNERTLYESGR
                     VWDIIAALKERDLAYEKDGATWFRTTAFGKEADTVLVKSTGEPTYRLPDIGYHVDKLE
                     RGFDRIIDI"
BASE COUNT         3302 a         7297 c         6953 g         3121 t
ORIGIN      
        1 cccatgaact cgatcgtgta gccgctgtac atgaccgaga cgatgggcgt gatggccagc
       61 gcctccgagg ccgcgagcga gtgggctgcc ccgaggccca cggcccaccc cgactcgctc
      121 cagtcgtccg gtgcttcgga aacggagacg aaatggtaat ggccctctcc gaaaatcgtg
      181 accggcatcg cctcactctg gcgcgcgaag tacccacgga cgcgtggcgt gatgtaggtc
      241 cggtttccgt cggatccgtc gtagctctcc ctctcgatgc cgagccctgc ctcgaagatc
      301 cggttgaccg cgtagccgac ggctccccca aagctcgacg cgttgtcgcc gctctggtag
      361 cccagctcga ccccgaaccc tctctcggtg tagattccct gtgccaacgc cgccggcgcg
      421 gtgaggaatg cgagcgcgac gatgacggac agtctggcat ggcgcatggt gttcacccgt
      481 ggattgttga agtgacctcg gaaggccgcg ctcgacaggg atggggtccg cactccggga
      541 gacgctcctc acacgtcccc gggcggcaga tgtacgaatg cagcgcgacg gactttacgc
      601 aatagcggtc aatccgtcaa atgccgcccg attggtccaa tcttcaatgc ggcccgcgtt
      661 gactcgccga acggggggcg gtagcttcga ttccgccaaa atgagcccga ttgcgggctc
      721 tgaacgggtc ttttcgccac cttcaaccct gccggcgacc catgctctcg gatttcctgc
      781 cgctcttcat gatgctcgtg atcgcgggcg gcgtggccct gatgctgctc aagtcggccg
      841 agctcgtcgg cccgaagcgg cccaacatca tcaagcagaa ggcctacgag agcggaatgg
      901 acccgatcgg cacggccagg gaccgctact cggtcaagtt ctatctcgtc gcgatgatct
      961 tcatcgtgtt cgacgtggaa gtcgtgttcc tgtacccgtg ggcggcgtcg ttcagcgaat
     1021 tcctggattc gggcgccgcg ctcggcgtga tgggggtcgt ggtgctgttc atcgtgatcc
     1081 tcgcgatcgg gctgctctac gacatccgga aagggggcct ggagttcgac tgacccttcc
     1141 cgcccgactc ggcaccttct aacagcctcc ctaccatggc acccgatccc gtaaccggag
     1201 gagagggctt tctcacgacg cgcgtcgacg ccgtcctgaa ctgggcgcgc tcgaattcgc
     1261 tcatgcccat gcccatgggc ctcgcctgct gcgccatcga gatgatggcc atggcggcgc
     1321 ccaagtacga cgtggcgcgg ttcggcagcg aggccttccg cttttcgccc cgccaggccg
     1381 acctgatgat cgtggcgggc tgggtcagct acaagatggc gcacaccatc cggcgcatct
     1441 gggaccagat ggccgatccg aaatggtgca tcgcgatggg cgcttgcgcc tcgagcggcg
     1501 gaatgcaccg ctgctacggc gtggtgcagg gctgtgacaa cttcctgccg gtcgacgtct
     1561 acatctcggg ctgcccgcct cgccccgagg ccgtcatcca cgcgttgatg gacatccagg
     1621 agaaggtgcg caaccagtac tccgtggccg cggacagcgc ctccggcgag gtgcagccgg
     1681 agccggccgc gctccggccg cgcgtgctca ccccgggcgg cgatcgggtg atttcgcccg
     1741 tggcactcta cgaccaggaa aacctgtagc aggccgatga cggacccggc acgccggcgt
     1801 tcgacttcgc atccgtcacc accatgaccc aaggccgcac gctcgcgttc cacttcacgc
     1861 cggtcgatgg gccgcgcggg accggggacg acaagaaccc gcacgccaag gacacgacgt
     1921 tcgtgccgga ggtcgtcgac gccctccggc agcgcttcgg agacgccgtc caggaggtca
     1981 tcctgcacgc caacgagcac acggtgtacg tggaccgggc ctccatcgtc gagatctgcc
     2041 gctacctcaa ggaggaccag cagttcgagt acctggcgga catcggcggc atcgacctct
     2101 tcaccgacga gaaccggttc gaggtcttct acaacctggt ctctatggcc cggggcaagc
     2161 gcctccggct gaagatccgc gtgcccgagg aagagcccgt cgtggcgagc ctcgtgccgg
     2221 tgtatccggc cgcgaattgg aacgagcgcg aatgctggga catgttcggc atccgcttcg
     2281 acggccaccc ggacctgcgc cgcatgttca tgccggagga tttcgagtac cacccgctcc
     2341 gcaaggaatt cccgctcctg ggcgttcccg gatcgctgcc cctgccgccg caggcgcccg
     2401 aggcgggcct caccctggac ccgttcgccg ccgcgcacgg ttcccggccc gtgaagagtt
     2461 tccaggaggc cccttcggac ctcgcgaaga agtagacgcg gtgcttccat gagcaagatc
     2521 tcgacagaga acttcggcga gacactgaaa ttctggccgc gccaccacga ggccctgtac
     2581 cgtcgcctgg agtcgaagca ctcctggctc gaggagcgcc atggcccgcg gcagaagggc
     2641 gactccgatc cgctcgagca cgagatggtg ctgaacatcg gcccccagca tccggcgacc
     2701 cacggagtgc tgcggtgcgc ggtcaagctc gacggcgaga ccatcgagcg gtgcgtgatc
     2761 gacatcgggt acctgcaccg cggcctcgag aaggtggccg agaacaagac ctaccaggag
     2821 ttcatgccct acacggaccg catggactac ctggcgccct acgccaacaa cgtgggctgg
     2881 tgcctggcgg tcgagaaggc ggccggcatc gaggtgcccg agcgggcgca gtggatccgc
     2941 atgatgatgt cggagctgtc gcgcatctcg tcccacctga tctggatggg cgtaggcctg
     3001 atggacgccg gggccgtttc ggtcttcctc tggacgttca agtaccggga ggacctctac
     3061 tcgatcttcg accacgtggc cggcgcccgg ctcacggtgt cccacagccg gatcggcgga
     3121 gtggccagcg acgtctcgga cgaggctatc gcgatgatcc aggccttcgt cgatgacttc
     3181 ccggcggcgg tcctgggctg ggagaagctg ctgaaccgga accgcatctg gatcgaccgc
     3241 aacgtcggga tcggcgtgct gacgcgcgag gacgcgctcg ccctcgggct cacgggtccg
     3301 aacctgcgcg gctcgggcat cccgcatgac atccggcggt tcgagccgta cctgaagtac
     3361 gacgaggtcg atttcaacat ccccatccgc gaggagggcg acgctttctc gcggtactac
     3421 gtccgcctgg acgagatgaa agagtcgctc aggatcgtcc agcagtgcct cgagaagctc
     3481 gagaccgtcc gcggcccgct ccgcaccgac gacgccaagc ggtcgtaccc gtccaaggac
     3541 gaggtgtact actcgatgga gggcctcatc cacgacttca tgtacaccga cacgggggtg
     3601 tgtccgccga agggcacgtg ggtgtaccac gcgatcgagg cgcccaaggg agagctcggt
     3661 ttctacatcc agtccgacgg gaccgggcat ccgttccggg cccgcatcaa cgcgcccagc
     3721 ttcaccaaca tccaggggct cgaggccctg ctggagggat cgatgatggg cgacatggtc
     3781 attctcatcg gcaccgtcga ccccgtgctc ggggaggccg acaagtaggt agcccatggc
     3841 tgatttcatc aagcatccgg tcgttccact ccccgaccgg gatcccgagc cggtgatccc
     3901 ggccgaccgg ctcctgttca ccgacgacga gaagcggagc atcgccgaat ggatctcgcg
     3961 gtacccctcc gcggacggcg ccatcatgcg cgcgctgtgg ctcgcgcagg agaagttcgg
     4021 cttcctgcca cccgaggtca tccggctcac ggcggaccag gtcgggatcc cgtacgccca
     4081 ggcttacggc gtggccacct tctacacgca gtactacaag aaggagatgg gccgacacgt
     4141 gctcgacgtg tgcacgtgct tctcgtgcca gctctgcggc ggatacgaca tgctccatca
     4201 cctggagagg aagctcggca tcggcaaggg cgagacgacc ccggacggcg cgttcacgat
     4261 ccaggaggtg gaatgcctgg gcgcctgcgg ctccgccccg gtgctccagg tcacgaacgg
     4321 tccctacgtg ttcaacctga ccgaggagcg gctcgacgcg ctcgtcgacg cgctccggga
     4381 ggaccggatt ccggagttca cgtccgtgac ccttccgcag gaccaggacg agctcggcgg
     4441 caaccgccgg tccgacgttc cggacacggt gggcagcctc acgccgcccg tctcctccac
     4501 gatccgctag cccgcgcgcg aaccgcaacc aggccatctc catggaagca tccaacggca
     4561 gcaaggcagg cgactggcgc tcgtaccggc gcgtgctcct tccaccggtc agggacctgc
     4621 accggctcga cgtgtacgag gcgaacgggg gttacggagc cctgcgcagc gtcctgaccg
     4681 gggcgaagtg ggatccgaag agcgtgaccg aggaggtcaa gaagtcggga ctcaagggcc
     4741 gcggcggggc cggattcccc accgggctga agtggacctt catgccgccc gtggatcccg
     4801 cgatcccgcg cttcctgtgc tgcaacggcg acgaatccga gcccggtact ttcaaggacc
     4861 ggcagctcat ggagtacaat ccgcacctcg tcttcgaggg catgctcatc gccgcgtacg
     4921 cgatgtcggt gaagacgtcg tacctgtacg tgcgcgggga gtacgcggac tggatcagcc
     4981 acatggagcg ggagctcgag aagctctacg cgaagggtta cgtcggaaag aacgtgatgg
     5041 ggtcggactt ctcgaccgac atcgtcatcc acaagggagc gggcgcctac atctgcgggg
     5101 aggaatcctc gctcatggaa tcgctcgagg gcaagcgcgc ctatccgcgc atcaagcccc
     5161 cgttcccggc ccagcgcggc atctggaatt acccgacgac gatcaacaac gtcgagacgc
     5221 tcgcgtgcgt cccgctgatc atcgagcgcg gcggggactg gtacgcgaag atcggcgcgc
     5281 ccacgcacac cggccccgtg ctgtacggcg tctccggcca cgtcaaccgg ccgggcgtgt
     5341 acgagtaccc gacgggcatg ctcatcaccg acctcatcca ggaggtggcg ggcggcatgc
     5401 gcggcggaaa gaagctcaag gccgtggttc cgggcgggag ctccacgccg gtgctccggg
     5461 ccgaccagat cgacggagtc accatggatc cggattcgct ccgcaccgtg ggctccatga
     5521 tgggcacggc cggaatgctg gtcatggacc aggacaccga catggtctcg ttcctgcgcc
     5581 ggatcacgca cttctaccac cacgagagct gcggccagtg cacgccctgc cgcgagggca
     5641 cgggctggct cgagaacctc gtgacccgga tcgacgaggg cgagggaaag ctcggcgacc
     5701 tggacctgct agtcgacatc tgctcgcaga tggagggccg caccgtgtgc gcgctcgccg
     5761 acgcggccgc ctggccggtg cgttacacga tcgagcgctt ccggagcgag ttcgaggcga
     5821 aatgccgccc gtcgctcgtt ccggcctccg gcattcccgt tcaccaggac tgaccccccg
     5881 cagatgacct gtacctcctg ctccatgacc cgactgctct acgcgctctt cgccctggcc
     5941 gtgctgctcg gcggctgccg ggccgacgcg cccgggaccg atgcgggcac cgccgaagag
     6001 gctgccgaga cgtccccggg atgggacgcc gtcggcgagg ccctggtcgc cgaaggcgcc
     6061 gtcctgacgc aggaccttgc cgcggatccc gagcccttcg tcggaaagga agtgacgatc
     6121 gcgggggtcg tggccgaagt ctgccagtcc tccggctgct ggctcacgct cgcggccggc
     6181 ccggaccgct cgccgatccg cgtcgacgtc ccccgcgatt ccgccggcgt ctacgtgtac
     6241 accttcccga aggacatctc ggggcggacc gtcgtcatcg ccggcatgct ccgggcggga
     6301 gacgaggcag agcacgagca cggcatgacg gaggacgccc acgcaacgga ggtggagggg
     6361 cacgacgaag ccgaggcccg ggccctcgcg ctggacctgg ccatcgtggc ccggggcgcg
     6421 ctcatcgagc ggatcggtcc ctgaccccgc atttcgaccc tgacctgaca tgcccacggt
     6481 aacgatagac ggcgctgcct acgagttcga gggtcgcccc cgactgctgc agttctgcct
     6541 ggaccacgga ctggagctgc cccatttctg ctatcacccg gccatgtcgg cgccggcgaa
     6601 ctgcaggcag tgcctggtcg aggtcggcat gcctgcccgc aaccgcgaga cgggacagcc
     6661 gcagctggac gatgccggca acccggtgat cagctggatg ccgaagctgc agaccagctg
     6721 ctcgctggac atggccgacg gcatggtcgt gcggtcccac cgctcgagtg acctcgtggc
     6781 ccgggcgcag aaggacaacc tcgagctcct gctcatcaac cacccgctcg actgcccgat
     6841 ctgcgaccag gccggccact gcccgctgca gatccaggcc tacaagtatg gccccgaggg
     6901 ctctcgcttc gagttcgaga agacgcacaa gaccaagcag ctggagctcg gcccgcgggt
     6961 ggtgctcgac ggcgagcgct gcatcaactg cacgcgctgc gtccgcttca ccgacgaaat
     7021 ctcgaagagc caccagctca cgatcatcga gcgcggcgtc cggaactacc cgatgacgcc
     7081 gcccggcacc atcttcgacg agccgtactc gatgaacgtc atcgacatct gcccggtggg
     7141 cgcgctcacg tcggtcgact ggcgattcca ggcgcgcatc tgggagatgt cgaagaccgc
     7201 ctccatcacg accggcaacg ccaagggctc caactgctgg tactgggtga aggacaaccg
     7261 cgtcatgaag gtcactccgc gtgacaacga gagcgtgaac gagtactggc tgcccgacga
     7321 ggaccgcctg gacaccgccc gtttcaacga ggaccggccg gcggggccgt ccgtggacgg
     7381 gtcgccggcg acctgggacg acgcataccg tgccgcggcc gcgctgcttc gcgacgtgga
     7441 tccgtcgaag gtggccttcc tcggctcggc gtacgcgacg gtcgaggaca actacctcct
     7501 cgcccgcctc gcgtcggccg tcggcgccga tgcgccccga tacatccccc acgtggagcc
     7561 cgggcacggc gacggctggc tgcgcaccga cgaccgcacg ccgaatgcgc agggctgcga
     7621 gcggctgggc atccatccgg tcgatgccgc gacgctgaag gcccgcctgg cgggtggcgc
     7681 gatcgacgtg ctgtacgtgc tcgaggacga tcccgtcggc tccggactct gcgacgaggc
     7741 cgacctttcc ggcgtgtccg tggtgctgca cccgtacaac acgacgaacc gcacgatggg
     7801 cgccgcccgc gtgacgcttc cggcggccac ggtcgtcgag accgtcggca cgttcgtcag
     7861 cgaggacggg cacgcccagc gcgtgcgacc ggccaaggcc atccggggca tcaaccggac
     7921 cctgatgatg gaggtgggca agagccgtca cgacctgcac ggcacgccgt tcgaccgctg
     7981 gttcagcgag gcgaacctcg tggactgcca gcccggatgg gtcagcctgc cgggcgtggc
     8041 cgcggccgcc ggccatccga tggactatca cgggccgcgc gccatcatgg acgagatcgc
     8101 cggcagcatc gatgccttcg cgtcggccac ctacgaggcc atgggcctgg cgggcgtccg
     8161 cctgcaggag gccccgaccc cggcatgagc gaacccctag ccatcccacc gctccgaccg
     8221 gccggacgtc gcgtatccgg cccacaacga acctgaccgt catggatcta gcctggtact
     8281 ggaccgccct catcgcgttt ctggtcgcga acttcttcct ggtctccgcc gcggtcctcg
     8341 tctacgccga gcgcaaggtc tccggctaca tgcagaaccg gcccggaccg aaccgcgtgg
     8401 gtccgttcgg cctgctccag ccgttcgcgg acgttctgaa gctgatcctg aaggagaaca
     8461 tcgtcccgac gagcgccacc gcgttcgtcc actcgctggc gccttcgatc atgatcctga
     8521 tcgccttcac cggcggcgcg ctgatcccgt tcgcccgcgg agtggtgatc gcggacatgg
     8581 aggtctcggt gctgatcctg ctggcgctga cgtcgatttc cgtgtacgga gtgacgctcg
     8641 cgggctggag ctcgaacagc aagtactccc tgctcggcgg cctccggtcg tcggcccaga
     8701 tgatctcgta cgagctgtcg atggggctcg cggtcgtctc cgtgatcctg ctcgcgggct
     8761 cgctgaacct ctcgaacatc gtcgagagcc aggccggcgg ttacggcatc ctggggtgga
     8821 acatcttccg caacccgatc ggggcggtca tcttcatcgt gaccgcgttc gccgagtgca
     8881 accggacgcc cttcgacctt cccgaggccg agcaggagct cgtcggcggc taccacaccg
     8941 agtactcggg catgaagttc ggcatgttct tcctggccga gtacgtgaat ttcttcatgg
     9001 cgtcgttcgt gatctcgacg ctcttcttcg gcggctacct gctcccgttc gagtccctga
     9061 tcctcggcgc cttccccgcg ctcgagggca gcgtgctcct gggcgtgctg caggtgctct
     9121 cgctgctggt caaggccggg ttcttcgcct tcacgttcat ctgggtgcgc tggacgctgc
     9181 cgcgcttcaa gtaccagcag ctgatgaagc tcggctggaa ggtccttctt ccgctcgcgc
     9241 tggtgaacgc gatcctcgtg gcgacgggca tcgcgatcgt ccgcgggttc tgatcgggcg
     9301 ccggttcgtt ccgattcagg gatcgtcgca gtaccttcgc tccgcgtccg ggcgggcccg
     9361 gtgcccgcgc ccccgcggcg gaccgtgcag ccgcccggac ggcccaccgc aaccgacctt
     9421 ccgcccgtgc cgattctcgc accctcgatc ctcgccgccg atttcgcgaa cctggagtca
     9481 cactgccggg aggcgttcga ggccggcgcc gagtggatcc acgtggacgt gatggatggc
     9541 cggttcgtgc cgaacatcac gatcggcccg cccgtggtcc acagcctgcg gccgttcgcc
     9601 gacgacaccg gcgcgctgct cgacgtgcac ctgatgatcg aggagccggg acgctacgtc
     9661 gaggccttcg cggcggccgg cgccgaccgg atcaccgtgc acgtcgaggc ctgcacccac
     9721 ctgcaccgta cgctgcagtc catccgcgag cgcggcgtcc gcgcgggcgt aacgctcaac
     9781 ccgggtacgc cggtccagag cgtggagccc gtgctcggtc tcgtggacct ggcgctcgtg
     9841 atgaccgtga acccgggctt cggagggcag gcgttcatcc cggagagcct gggccgcatc
     9901 cgcgcggtcc gatcgatgct cgacgccctg ggctcccgcg cccacgtgca ggtggacggc
     9961 ggcgtcacgc ccgaaaacgt ggcgaccgtc atcgaggccg gtgcggacgt ggtcgtggcc
    10021 ggcagcgccg tgttccgcgg caggggcacc gtcgccgaga acgtgcaatc ctttcgccgc
    10081 gcatggtctc tttcgacatg aacgaggtcg agggaatgac gtgcgtcgtg acgggcgcga
    10141 cgtcgggcat cggccgcgtg gcggccgccg agttcgcgcg gtgcggcgcc cgggtcgtga
    10201 tcgtgggccg ttcgcgggag cgtggccggc atacgctcgt ggaaatcatg cgcgacacgg
    10261 gaaacaccca ggtggacctg atccttgccg acctctcgtc ccaggccgac gtgcgccggc
    10321 tcgccgcgga catcacggcc cgctacccgc agatcgacgt cctggtcaac aacgcggggg
    10381 ccatcttccg acgccgggag gagtcggtgg acgggatcga gatgaccctg gccctgaacc
    10441 acctggggta cttcctgctc acgaacctgc tcctcgacac gctccgcagc agtgcaccct
    10501 gccggatcgt gaacgtggcg tccagcgccc accgccgcgc ccggctggac ctcgacgatc
    10561 tgcagtcgcg gcgccgctac cgcgcgtggc gggcctacgc gaactcgaag ctctgcaacc
    10621 tgctcttcac gtacgagctc gcccggaggc tggagggcac cgggatcaca gtgaacgcgc
    10681 tgcacccggg gaccgtcgcg acgcgcttcg ggagcaacaa ttccgggccc atgcggcgga
    10741 tgatggtgtt cgggcggccg tttctgcgga cgccggagcg cggggcggag accatcgccc
    10801 atctcgcgct cgccccggag ctggcctcgt cgaccggcgg ctacttcgtg gacgaacggc
    10861 cgcgccggtc ctcccgggcg tcccacgatg cggccgtggc cgcgcggctg tgggacctct
    10921 cggccgagct cacgggcatc ggccggtgag ggtcagcggg aattcccgct cccgagcgtc
    10981 atagagagct cgacctgctc gccgccccgg atcacggtga accgcacctc gtctcccggc
    11041 cggaagtcgt agagccgctg cacgacgtcg ttccggttcg ccaccgcctc cccctcgacg
    11101 gccacgatca cgtcgtacgg gccgaacccc gcatcctcgg ccggagagcc gtactcgacg
    11161 tcctcgatga acacgccgcg cgcctccgga agccggagcg cctgcgcgat acgcgccgtc
    11221 acctcgcgga cccgcagccc ggtgtagtac gagcggtcca cgaagccctt gtcacgaagc
    11281 tcccccacca ccttcagggc acgctcggcg ggcaccgcga acccgatgcc cacggacccg
    11341 cccgccggcg tgacgatcgc cgtgttcacg ccgatcacct cgcccgccgc gttgagcagc
    11401 ggcccgcccg agttgcccga attgatcgcg gcgtcggtct ggatcatgtc ctggtacatg
    11461 tgcccttcct tgggcgagag gtcccgcccg atcgcgctca cgacgcccac ggtgacggtc
    11521 gggtccgacg cctcgaagag cccgaacgga ttgcccatag cgatgcacca ctcgcccacg
    11581 atgggcgacg ggtccgtcga aaacgccagg tacggcaggg gctcgtcgga ctccaccttc
    11641 agcagggtca ggtccgaggc cttgtccgtg cccacgagcg acgccgtgtg ggttcgaccg
    11701 ttcgtaaacg cgacggtgat ttcgtccgcc ccggccgcga cgtggtcgtt cgtcacgatg
    11761 tagccgtcgg gcgagatcac gaagcctgac cccatgctct ggacgcgccg ctcccgcatc
    11821 ctggagcgct ggcccccgaa gaagaagtcg aagatcggat cgttgaacgg gtcgctgtag
    11881 acggtccgct tgatggcgtt caccgaaacc acggcgggcg aggccacctg gacggcggtc
    11941 gtgatcgcgt tctggcggct ccggccgatg tcgtcctgtc cgaggagaac ttcggcgggg
    12001 acacgctgcg gcggcagggc ctgctcctgc gactgcacgt tccggttgca gccgacaagg
    12061 agcagcagcg cgagaatgtg gaacgacggg cggcgcatcg aggcgggagt gggttcgcga
    12121 ccgctgttac ggagcccccc tgcgcgggtt tctggtcgtg cgtggcgcca tcggaatggc
    12181 ccgctccgta tcatcctccc gtccatgacc tgttctgcag tgcccgaacc acggatcgac
    12241 gccgcccgac tcgtccgcga cctggagcgg ctggcccgcg tcggcgcccg gaccgacggc
    12301 ggtgtgaccc gcctcgcctt ctcggccgag gaccgggcgg ggcgggacgt cgtgcagaag
    12361 ctgctcgcgg agctcggcgt cgcgtcccgt atcgaccggg tcggcaacct ggtggggatc
    12421 cgcgcgggga ccgaggccga tgcgcccgtc gtcctgaccg gctcgcacac ggacaccgtc
    12481 gaggcggggg gacgattcga cggcgccctg ggcgtgcttg ccggcgtggc ggtgctgcgc
    12541 gcgctcgatg cggcgggcgc cctgacccgg cggccggtcg gggtggtctc gttcgtgaac
    12601 gaggaagggg tgcgcttcat gccggacatg atgggcagcc tgttcgtgcg cggggacctg
    12661 tccctcgccg acatccacgc cgccgtgggc accgacgggg ccacgatcgg ggacgagctc
    12721 gcgcggcacg gcctgtcggg ggacgacgac ttctcggccc ttcccgtttc ggcgttcctc
    12781 gagttgcaca tcgagcaggg cccagtgctc gacgccgccg aggagccggt cggggtcgtc
    12841 acgggcgtgc agggcctccg ctggctccgg gtccgcgtct cgggcgcgtc gaaccacgcg
    12901 ggcacgacgc ccatgcgcgg gcggcgggac gccgggctcg tggcggcccg catcgcggcc
    12961 ggcgtccggg agctggcgat gacgatggcc cccctccggg cgacgtgtgg ccgcctggtg
    13021 ctcgagccga acgtcgtgaa cgtcattccg gcctcggccg agctcaccgt ggacctgcgc
    13081 caccccgacg cggacgggct ctcggccgcg gaggccgccg tgcgggccct cgtggcgcgg
    13141 gaggcggcag gcgagaccgt gcacgccgcc gtcgagcccc tcgcccacgc gccgcccgtc
    13201 accttcgatt cccggatcgt ttcggcggtc gagcgggccg cggcagcgct cggcctgccg
    13261 tgccggcgcc tcctgagcgg ggccggccac gacgcccaga tcctgagccg agcctggccc
    13321 tcggccatga tcttcgtccg gagccggaac ggcatcagcc acagcccggc agaatggtcg
    13381 tcgccggacg acctggtcgc cggggcggag ctgctgctcg gaagcgtgct ggaactcgcg
    13441 ggctagtccg ccggggcgtc gcgatgcgcc ctcacgtccc cagcgcgcgc aggacgagcc
    13501 gcgcggcgac caggaagacg agcaccgcga acgcccgctc gagccaccgg gtattgatgt
    13561 gatgcgccgt ggtcacgccc aggcgcgcgg cgcccgcggc cgcgaccgac aggacgagcg
    13621 ccgcccccac gtcgacgtac ccgagcgccg tcggcggaac cggcgcggtc caccccgtcg
    13681 cgacgtatgc caggacgccg atgagcgcga tgaacacgat cgatgcgctg gacgtgccga
    13741 cggagcggtg catggacagg tggagcagct cgtggtaggc cgggacgagc accaccccgc
    13801 cccccacccc gaccgccgcg gagaccgctc ccgcgaccga ccccatgccg gccaggagaa
    13861 gcccgcctcc tcccctcgcg gatcgcccct cggccgcgcc cccgcgctcc ggccgggcga
    13921 ggagcatgcg cagcccgacc cacacgagga ccgccgagaa gaccacctgg aacaccgtgg
    13981 cgtcgtacca cggccgcgtg gtcacgaacc acgtcatggc cagcactacg accgcgctcg
    14041 agagccccgt cagggtggca attcgccagt ccaccgccgc gcggaggtgc tgggctctcg
    14101 cgctcacgcc ggaggtcacg agcgtcccga aaaggctcga gccgatggtc agcggggtca
    14161 gcgtcggatc ggtcacgccc tgggcctgca ggtagaagaa gagcaccggg gcgaagatca
    14221 cgcccccgcc cacccccacg agtcccccca cgaatccgcc ggcgaggccg gccgtcgcca
    14281 gcacggcaac atccatccat tccatgccgg tcacctcgat ccgagagcgg cgtcgtaccg
    14341 ctcccggctg ccgaccacct ccagggcccc gccgagcacg gggtcgtcgg agagcgacca
    14401 ccggacctgg tcggtggatc ccatcaggcg accggcgact tcatcggaaa gccgcgagac
    14461 gagccgctcg cgctccctct cgaacgcccc gtcacgctcg cgggcggcag cctcctgcag
    14521 cgcgtcccac gcctggcgga cgggcggcgt ccgatcgtcg gccgacagtt cggaaagcga
    14581 gcgctcgaag ggggtccgga actcgaagtc gcgctccgcg agccagcgcc ggaagcgctc
    14641 cagggtcccg tcgtcgatcg gcaggggacg gccggcctcg agggcggcct cgagctcggc
    14701 gccctgctcg gccacgatca cgcctgcgaa caggaaaaac gcggcctcgc gctccagggc
    14761 ttcctcgagc gggccctccg cggcgtctgg cacgagcacc tcgggctcga cgccgtaacc
    14821 ctcgcgcacc gtccggccgg ccgccgtgaa gaactcctcc cgggacggcg cctccgcgtt
    14881 ctcgagcgcg tccttgcgga tgccacggcc gctcggcgtg tagtagcggg ccatcgtcag
    14941 cttgagcgac gtgttgtacg gaagtccgcg gaagacctgc acgaggccct tcccgaagct
    15001 ggttgatccg accacgaccg cccggtcgtg atcctgcagg gccccggcga cgatctcgct
    15061 cgccgaggcg ctgtacccgt tcacgaggac ggcgaccggc acgtccggaa tgaccggatc
    15121 gccctgggtg tggtaggccc ggttcgactc gggcgcgcgg ccgcgcgtgg acacgacgag
    15181 cgtgcccagc gggaggaaga gctgcgagac ggcgatcgcc tggctcagga gaccgcccgg
    15241 gttgttccgc aggtccagga tgacggcccg gaccgggccc gcggcctcga tcccgcggta
    15301 cgcggcggcg agctcggcgc ccgcggcctg gccgaagcgg tcgagcttca ggtaggcgat
    15361 gtcgtcgttc ggcgcgggca gtcgctcggc gagggacacg ttgcgcgcgg tcggggcctc
    15421 gcgccgcaga cggaagacca ggacgcccga accctcgcgc tcgatctcca cgtccaccac
    15481 ggagcccgga tccccccgca agagctcctg gacgtccggc accgagaggc tgtccacgct
    15541 ctgccctccc acgctccgca cgacgtcccc ggtccggatg ccctggcggt acgcgctcgc
    15601 cgcctcgtcc ggcaccagga ccgtgatccg gcctccgcga cgtcccaggt tcaggccgac
    15661 gtcgccgagc agccgccgcc ccctgaggtc gatctggacg ttgtccgcct cgtccaggaa
    15721 gtcggtccag ggatcgagcg tgccgagcat cgcgtccatc cccgtgcgca tgaaccgctc
    15781 cgggtcgatg cgcgtcacgt aggtgcccac gagctcctcg tacaccgagc cgaagagctc
    15841 gaaattcttg cgcatcgcgt agaaatcgtc gtccggcggg cgggatccgg tccacaggac
    15901 ggagccgccg acgaggagca gcaacagggt ggacgaagcg tacagcggtc gcatgcggca
    15961 ttcccggtga gtcagcccgt ctgacgccgc tccccggaag gagttcgacc cgcccggctc
    16021 aggcgagcgc ctgggccagc tcgcgctcgc ggcgacgggt gacgaccgac gagatccaga
    16081 tccccagttc gtacagcagg acgagcggcg tcgcgacgat cacctgcgag atcggatccg
    16141 gaggcgtgaa gatcgccccg agcgtgaggc agatgatcag gttccacttg cggaaccccc
    16201 gcaggcgccc gggggtcgcg atgccgagct tggccaggaa atagatcacc acgggcagct
    16261 cgaacaggac gccggccccg aacgaccacg tggtgatcat cgacaagtat ctcgaaatgt
    16321 cgaactcgtt cgagatctcc gcagagatcg agtagcccgc gaagaactga agggccagcg
    16381 gagtcacgat caggtagccg aacgagatgc cgaaggcgaa gaagaacgtg gcgaacaccg
    16441 cggcgaagcg catccccttc ttctcgctcg ggtacagtcc cggctcgacg aagcgccaca
    16501 tctggtagac gaacacggga gagcccagga tgaccccgac cgcgatgatc gtgcccatgt
    16561 cggcgaagaa ctgcccggtg atggtgcggt tctggagcac gaactccgtc gcctcgaccc
    16621 cgaagagccg gtacatgaag aagtccggcg acttgggggc gagcagcagg ttctcgatga
    16681 tccaccgccg gaagaagatg gcgccgatcg tgaagaccag gatgccggaa aagcccttga
    16741 tcaggctcca ccggaggtcc tccaggtgat ccaggaagcc catctcgagc atcccctctt
    16801 ccggatccac gagcgagccc tgctcccggg cagccttcag ctgggccgca cgcgcctcct
    16861 tccgtctccg aagtcccatg agcatgtcgt ggatgcggac aaacggactc accctaccag
    16921 gacgagtcgg aggagccggg ccggtgatcc cggaggttca ggtcgagcag cgagtccacc
    16981 cacaggccga gctcctcgac ccgtgcgcga cctccgctcc acgtgaagtt gaagagcgtc
    17041 aggaggcctg cgacctggaa gccgtcctgc cggagcagac ccacggcccg ggcggcgctg
    17101 cggccgctgt tcagaatgtc gtcgaggacg acgaggggac gggcgcggtc gatcggtccc
    17161 tccacgagtc tgcggcggcc gtgcggcttg cgatgctcgc ggacgaaccc gcccgagagc
    17221 gaagggcccg tgccggacga gagcaccgag cacacgagtg cgtacgagcc gaatccgaag
    17281 cccgctacct ggttcacacc gcgcgaccgg acccgatccg caagcacgtc cccgacctcc
    17341 cggaagagca ccccgtccag catggccatg cgggtgtcca ggagccagcc gatcgcctgc
    17401 ccgcggggat cggtgatgag ctcctgctca cggcggacca gggcgcggtc gtagagtcgg
    17461 cgcccgagat ccaggagttc ggcgtatccg gcactgcccg tcgggatgtc ggtgagatct
    17521 tcggtcacgg gagtggagga ttggggcatt cggccggaat cgaaggatag tacctctggc
    17581 ggcgagcgct tgatccggaa tcggtcagac caccacgaaa tcgtacagct gaaagtcccg
    17641 gcacatcatg cggacctcct cgcgcacgga cgcgatgacg gactcgtcgc ccggggcgga
    17701 cagcacgcga tccacgagct cgaccaccct ggagaattcg tccggaccga agccgcgcgt
    17761 ggtcatggcc ggcgtgccca cccggatgcc gctcgtgacg aacgggctct ccgtgtcgaa
    17821 gggcaccatg ttcttgttga ccgtgatgtc cgcgcgctcc agcacggctt ccgcgtcctt
    17881 cccggtgatg cccttgcttc gcaggtcgat cagcaccagg tggttgtccg tgccgcccga
    17941 caccaggtcg tatccccgct ccatgaaggc ggtggccatc acctgggcat tccgcacgac
    18001 ctcccgggca taggtccgga acgaggggtc gagcgcctcg gcgaacgaca cggccttggc
    18061 cgcgatcacg tgcatgagcg ggccgccctg catgcccggg aatacggccg cgtccagcag
    18121 ctcgctcatc atcttcacgc gcccggactt cggtgcgacc ttgccgaacg ggttctcgta
    18181 gtcacgcccg agcaggatca tgcccgccct gggcccgcgc agggtcttgt gggtggtggt
    18241 cgacaccacg tggcagtgcg gcagcggatc gttcaggacg tcggcggcga tgagtcccgc
    18301 ggtgtgcgcc atgtccatcc acaggaacgc tcccacctcg tcggccacgg agcggaacgc
    18361 cgcgtaatcg aagtcgcggc tgtaggcgct cgcgccgatc gagatcatgc gcggctgcac
    18421 cgccttcgcc ttgtcccgga cccggtccat gtcgatccgg cccgccatcg gcccgtcccg
    18481 ctccacgccg tagtgctcgg cgcggtacag gatcccggag aaattgaccg ggctgccgtg
    18541 ggtcaggtgc ccgccgtgcg ccaggtccag gcccaggaag gtctcgcccg ggttcatgaa
    18601 ggccaggtag acggacgcgt tggccgacgc gccgctatgg ggctgaacgt tcacccattc
    18661 ggccccgaac agctgccggg cgcggtcgcg ggccaggtcc tccacctcgt ccacgaactg
    18721 gcatccgccg tagtaccgct tgccggggag cccttccgcg tacttgttcg tgagcgggga
    18781 tcccatggcc tcgagcacgc ttcgcgacac gaagttctcg gacgcgatga gctcgagccc
    18841 gtcgttctgc cgcccgacct cccgggcgat cgaagcatac acctcggggt cgtgggactg
    18901 cagctcggac atggacgcgg gagtcgggtg aacgaatgcg ggccttccgc gcgctgcgcg
    18961 gcacggccgg gaagctacgg cacgggcatc aaaccccgcc cggggccgtc actgcagaat
    19021 ctcggggcgg ctcagctcga aaaaggagcc ggcgaccccc acgaaacgcc actcgtcctc
    19081 ctcgcggccg tcggccccga ggtccacccg gaagatgctg aagagcaggt agtcgtcccg
    19141 ctcggcggcg cgggcggcga tcgcgccggc cacgtcgctt ccgaacgccg tgacgatgcg
    19201 cccgacccgc tcggtgccca ggtccgaact gagccgcttc tccacgcgct cgcggacgaa
    19261 ctcacggaat tcggactcgc ccggattgta gcgggccagg aagccgccca cggcgagggc
    19321 gaggacgacg acgatgagga atctcatgga tctcggagtt cggtgcgtcc ccgcacgggg
    19381 cgggagccgg tgggcgtctc cgtcctccgg cgcacgacgg acggccccct tacgaccccg
    19441 gcgacggggt ggatcggact atacgaccgg gcggcctcca ggtttgcgcg ggggcggagc
    19501 cgccgccgac cggccgggcc ggattcgctc tccgagagac cgcttcgtac ttttcggccc
    19561 caccgcgccc ggcgatccgc cgggcttttt tgcacccgaa gctccccggc ggccgcgggc
    19621 cgcccgatcg tcgacccgag cgcatgcgcg attacctcgc aagccggatc ctcgccgccc
    19681 tcgagaccct gggcggcgcc ccggacggct tcgtccctga attccagcag ccgtcgaacc
    19741 cggagcacgg ggacctcgcc tcgaacgcgg ccctgcagct ggcccgccat ttccggaagg
    19801 ctccccggca ggtcgcggag gatctctcgg gcgcgctcga cctggatccg cgccggatca
    19861 cggccgtgga aatcgcgggg cccggcttca tcaatttccg cttcgggacg gcctggctca
    19921 cggacgagct ctcccgcatc gtcgaggcgg gcgagcgcta cggccggacg gagggcggcc
    19981 gcgggaaatc ggccatcgtc gagttcgtga gcgccaatcc gaccggcccg ctcaccgtgg
    20041 gccacgggcg caacgcggtg ctgggcgaca ccgtcgcgaa cctcttcgac tggacgggct
    20101 acgcggtcac gcgcgagtat tacttcaacg acgcgggccg gcagatgcgg atcctgggcg
    20161 agtccgtccg ggcgcgctac ctcgccctcg tggacccctc gcgcggcacg cgcgagatcg
    20221 acgggccgtc cggaccggtc accgtcccgg ccgacttccc ggaggacggc tacctgggcg
    20281 agtacatcgg cgacatcgcg cgcggcctct tcgacgcgca cggagcggac ctcgccggtg
    20341 ccgatgccga gggtcccttc aaggaggcgg ccgaaaaggc catcttccgc gagatcgagg
    20401 cgacgctcgt gcgcctcggc atccggatgg atacctactt caacgagcgc acgctctacg
    20461 agagcggccg cgtgtgggac atcatcgcgg cgctgaagga gcgcgacctg gcctacgaga
    20521 aggacggcgc cacctggttc cggaccaccg cgttcggcaa ggaggccgac accgtgctgg
    20581 tcaagtccac cggggagccc acgtaccggc tgcccgacat cgggtaccac gtcgacaagc
    20641 tcgagcgcgg cttcgaccgc atcatcgaca tct
//
LOCUS       DBNHQZ010000369        12426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1736838, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000369 DBNHQZ010000000
VERSION     DBNHQZ010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1736838"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="AC16YU_09985"
     CDS             <1..686
                     /locus_tag="AC16YU_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR6065598.1"
                     /translation="PFCQRPIEHGADFVVHSLTKGLSGFGTDMGGVVIGPHSHYTDLL
                     MYRKDFGGVLSSRAAWSVLAYGLPSLAVRVRHAQGTALHIARHLENHPAVSHVAYPGL
                     PSFVGFEVAQRQMRDYDGNFAPGTLIYFELKGATPAERQRAGAAFINHLAENAYTITL
                     AVSLGNVRTLVEHPSTMTHAPIPPEEQLRAGIEPSGIRLAIGLEAVDDLLRDLESAIE
                     AARRVTEPA"
     gene            complement(694..2412)
                     /locus_tag="AC16YU_09990"
     CDS             complement(694..2412)
                     /locus_tag="AC16YU_09990"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6065599.1"
                     /translation="MSPRTSALLLAGLLAACGTSTPDNPATLVLHGGRIVTLDDERPE
                     ATWLAVRGTTIAVVGDSADVAGLIGPNTEVLDLGGRLAVPGFIEGHAHFMGVGRAKMV
                     LDLTQATSWQDIVDQVGEAASEAEPGAWILGRGWHQEKWDETPEGSVDGVPTHHALSA
                     VSPLNPVELGHASGHATFVNAEAMRLGGISAETPSPEGGEIVRDARGEPTGLLRENAQ
                     GLVGRARAAAEMNRTAEEAIAEARERARLAAEEALSKGVTSFHDAGSGFETVDLFRTL
                     ADEGRLPVRLYVMLSAGNDALEERIEDYRLVGYGDHHLTVRSIKRSIDGALGSHGAWL
                     LQPYADKPESSGLNTEDPAAIARTARIALDHGFQLNVHAIGDRANREVLDLYERAFDG
                     PDTLDRRWRIEHAQHLHPSDIPRFAELGVVAAMQGVHATSDGPWVIAKLGEQRALEGS
                     HVWRSLMDTGAVVTNGTDAPVEDVDPIASFHSTVTRRTRDGSAYYPGQAMTREEALRS
                     YTLLNAWAAFEEDIKGSLTPGKLADIVVLSKDILTVPDEEILSARVDVTIVGGRILYE
                     RTAAPD"
     gene            complement(2409..2954)
                     /locus_tag="AC16YU_09995"
     CDS             complement(2409..2954)
                     /locus_tag="AC16YU_09995"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR6065600.1"
                     /translation="MVASIAVFCGASRRIPEAFAREAETLGRLLAENDFGLVYGGGDI
                     GLMGALARAVHAHGGHVAGYIPTSLRQREGVAYTVADELHVTADLFERKREIFARANG
                     FVVLPGGLGTLEEFLEIVTLRQLGDHDKPVVVVNCRRYFNPLFDFLEEATELGFMPET
                     RSLFTVVDDPESAILALKSGI"
     gene            complement(2962..4068)
                     /locus_tag="AC16YU_10000"
     CDS             complement(2962..4068)
                     /locus_tag="AC16YU_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6065601.1"
                     /translation="MKRRAFIRKGSAIAAGGALAAGCAGGPGEGGAPGVQTRPRLSWR
                     LVSSFPRSLDTIYGAAEVLSRRVEALTDGRFTIRVFPAGEIVPYDQVLDSVGRGTVHM
                     GHSASYYFVGHNPALAFDCTVPFGLNARQFNAWLYHGEGMELTRSMFADFNVVNLPGG
                     NTGTQMGGWFRREIAGLSDIRGLKIRIPGFGGRVLDRLGATVQVLAGGDIYPALERGA
                     IDATEWSGPYDDEKLGFHKVAPYYYYPGWWEPGPNLSFYVNRAAWDGLPSDYRAALEA
                     AAAEANVDMMARYDAQNPPALERLLAGGTELRRFSDDVLQAAARISEEIMHEEASDPA
                     YRRIMESYVAWRHASNGWLRTAEWSMAIYHSNQA"
     gene            complement(4134..4853)
                     /locus_tag="AC16YU_10005"
     CDS             complement(4134..4853)
                     /locus_tag="AC16YU_10005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6065602.1"
                     /translation="MTLENRTAVVTGASAGLGAAISRDLVHRGATVIGLARRMDRLEA
                     MRTELGDRFIPVPCDVTDEASVERAFTAIRGLGVSVDILVNNAGLGLFGEVEALRLDD
                     WDVMMNTNLRGLFLCTRAVLPGMRAKNEETGFGGHIVNIASVAGLIGNPRIAAYNASK
                     FGVRGFSEALMKEVRDQGIKVTCVYPGSIATEFQEVAGTSGTSNPMTADDVAGTVSHV
                     LMTAENYLVSEVVMRPLRPRG"
     gene            5071..10653
                     /locus_tag="AC16YU_10010"
     CDS             5071..10653
                     /locus_tag="AC16YU_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024972.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR6065603.1"
                     /translation="MLRRPAILLPLLFAASAVAQPAEFLPETLYAIGGASYDVRSVDL
                     NGDGAPDIVSSAQGDGGVSVLINQGDGTFAPSVFYLVATGIRSLSVGDFDGDGHRDVV
                     ASANVNTIWFLPGAGDGTLGAPVSTVDNDMSGNATDLAEADFDGDGTLDVVSLAAGTA
                     FVMTGDGDGTFTRTDFVMLDGGHFLYGRLVAEDLDGDGHPDLAVANGDYKVLVALNDG
                     TGDLGAAVVISEDIEAFSVAAGDFDGDGDPDLVAAGMGWIQTFANDGSGAFVLSGEAN
                     ASGSSLGTAVATADLNLDGHLDVVVTAGSVGLNPYYGAGDGSFAAGATIATSLDNHFG
                     MLADDLSGDGKPDLAATDTGVLENALVVRLNATEPASSAVAADDHYVTAVGQALSIPA
                     PGVLANDAGAPPLQAFPLGFPSAGSASLSLDGSLAYIPDPGYEGTVSFTYELTDGGGQ
                     TDQATVSITVTSVEPLLMATSIDIAPMDPWVTRRRTGEPNIDHLAELAAGGGGEVLLN
                     FFGDVQVLAYQDGVEWRDEARFTWFGRRVGNERDHVILVVDLNLGTVTGNATVDGRIY
                     QVRPVSTWHEAREIDQAGFPEEEDDAPEDEEDIAGKTGGGAAWPGALLEPGNSHPIID
                     ILVVYTDDTAAASPDILSEIQLAFDETNLGFAQSGINAEVRLVATELVNYEETGNATT
                     DRTRIKRTSDGHLDHVHALRDQVGADVVSFWVEDGGSSKCGVAYIMKQVDGSFRDRAF
                     FSVVRGCATGNFTFGHELGHVMGARHDGFVDPFNVPYPYSHGWLDVGDRWRTVMSYND
                     ACDCSDEPVQPCPLKAFRSTKNAPVCSRVLFWSDPDGSFDNPNRADPGGAMGLADKAD
                     NVRALNNTAATVAAYSDAVFALVRGRVTHQVSGDSLEGIQITGFPSPVVTDTTGFYET
                     LVPLHWSGTLEARNDTLAFVPPSAYIADATGNIEQDFEGIERTVSGHVWIENTSDFVQ
                     GVVIEGFDEPVETDADGHYEIIVPHEWSGVLVPKLEPYRFDPPSVAISSVIAPTMQDF
                     VALLPPIVSGTVTESGTGAAIEGAWLSGCPVEVLTDAAGAYACPVEFGWSGTLVALAA
                     GYNMTPETRSYEAVWDDLSGQDFVGEPGVLANSDWPLTGRDQARTAGGTGSPVRYGTR
                     WTYEDGSRWPSSPVVGPGGLVYFGTANGRLRALDPTGSPRWLALPSSPPDAGGLAVGA
                     GGVLYMTQSSLAHLSSGTVSAVLPDGSGTLWSTSFQERVDVAPNLGASGTLYVGTSQG
                     DLVAYDASDGTERWRFETEGGAVSSAALTGDETIVVGTQAGRVYAVNPDGTLGWMHDT
                     GFTSSSAPALAPDGTVYIGSDDHRLYAVGADGALKWSFATLGPVRATPALGPGGEVYV
                     GSDDGHLYAISSGGQLRWKYRTGGPVTAGAVVSTHVSPFDPLLPGVEQTETVVYFGSG
                     DGTVYAVNAGGTFTASMPGTLRWSTATDHPIEYAPALDFARQTLYVSSGPRLWAFGGQ
                     PSGGTGLVQITSLGTVLDPEILELRDNIHILVALAGEGGFGGSAILGGLPEDPCQGGP
                     LFIPDCQDLASGLGTATPLVEVPSLVDLQIGFVKEGFGQAVLGGDPDGLLSTYTTVLR
                     DGGTYTGILAGTTHPELLAPNPDGLPTRLTVFEAPTGTGSGTGEPVYVGHFSTDLGTV
                     EIRLTGSGTYTFGPLRYGELSDLALVEPGAYEIQVVPLDGVAGKRQAALVASLDLAAG
                     GPTGILLAGFADPEANLGGPGLTMYRADGGGGTDVIGVAVGPRFELPRHVTLEPAYPN
                     PFNPVTVIAYALPDERDVRLEVFDVLGRSVARLVDGRKAAGRHEVSFDAGRLAGGVYL
                     VRLMAGGQVATRAVTLLK"
     gene            10693..11457
                     /locus_tag="AC16YU_10015"
     CDS             10693..11457
                     /locus_tag="AC16YU_10015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6065604.1"
                     /translation="MTPVESGKDAVDRLLAEARPASDGFTAAGRRLEPDDVVRRLAPF
                     LSGERRRRIESVLDGRTYAVATVVEGLSNSGNVSAVMRTAEALGFQRFDLVTGSAPFK
                     HSRRTTQGAQKWLDVHVWPDPDACVGALKAEGYRVVAAHLGAGAEPVDGVDFTVRTAL
                     VFGNELSGVSERMLQLADARCILPITGFAQSYNISVAAGMALYRAWQDRTARLGRCGD
                     LAAGDRERLRAEFHVRALRNARAILERSIREEAADG"
     gene            11450..11986
                     /locus_tag="AC16YU_10020"
     CDS             11450..11986
                     /locus_tag="AC16YU_10020"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6065605.1"
                     /translation="MADWWPPGALAGRVLDTPVGPVTIAATAGGVAGIRFGRVAMPPD
                     PGARAPASARTAVGMVERASRQLMAYFDEGSTGFDVPLDRTGTPFQLRVWEAIASIPY
                     GATATYGQVARRLGDVRLARAVGAASGANPVPIIVPCHRLVGSDGSLTGFGGGLDVKA
                     LLLRHEARAVQPDLFGGP"
     gene            12032..>12426
                     /locus_tag="AC16YU_10025"
     CDS             12032..>12426
                     /locus_tag="AC16YU_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065606.1"
                     /translation="MRRFLPLSVLLALTGCGGSAPVVEPPPPAPVVEPPPGPAFVVAV
                     VERGQVAVHDLRTGARRTVGGFGGEVASRHAAPAGSSSVVAIADADSVRLVHSDPARG
                     TLRVVASRPVPVRYTVAWASDGSAFAYGY"
BASE COUNT         1741 a         4356 c         4462 g         1867 t
ORIGIN      
        1 cgcccttctg ccagcgcccc atcgagcacg gggcggactt cgtcgtgcac agcctgacga
       61 agggcctgag cgggttcggc acggacatgg gcggcgtcgt gatcgggccg cactcgcact
      121 acaccgacct gctcatgtac cgaaaggact tcggcggggt gctgtcgtcg cgcgcggcgt
      181 ggagcgtcct cgcgtacggc ctgccaagcc tggccgtgcg ggtcaggcac gcccagggca
      241 ctgccctcca catcgctcgc cacctcgaga atcacccggc ggtctcgcac gtggcgtacc
      301 ccgggctgcc gtcgttcgtg ggcttcgagg tcgcccagcg gcagatgcgc gattacgacg
      361 gcaacttcgc ccccggcacg ctgatctact tcgagctcaa gggtgcgacg ccggccgaac
      421 ggcagcgggc gggagccgcc ttcatcaatc acctggccga gaacgcgtac acgatcacgc
      481 tcgccgtcag cctggggaac gtgcggacgc tcgtcgagca tccgtccacg atgacccacg
      541 cccccattcc gccggaagag cagctgcggg cgggcatcga gccgtcgggc atccggctcg
      601 cgatcggtct cgaggccgtg gacgacctgc tgcgcgacct cgagtcggcg atcgaggcgg
      661 cacgccgggt gaccgagccg gcctgacgga cggtcagtcc ggggcggccg tccgctcgta
      721 caggatccgg ccgcccacga tcgtgacgtc cacgcgggcc gagaggatct cctcgtcggg
      781 cacggtgagg atgtccttcg agagcacgac gatgtcggcg agcttgcccg gcgtgagcga
      841 gcccttgatg tcctcctcga acgccgccca ggcgttcagg agggtgtagg agcgcagggc
      901 ctcctcgcgg gtcatcgcct gcccgggata gtaggccgag ccgtcgcgcg tccggcgggt
      961 cacggtcgag tggaaggagg cgatggggtc gacgtcctcg accggcgcgt ccgtcccgtt
     1021 ggtcaccacc gccccggtgt ccatgagcga ccgccagacg tgggagccct ccagggcccg
     1081 ctgctcgccg agcttcgcga tgacccacgg accgtcggac gtggcgtgca cgccctgcat
     1141 cgccgcgacg acgcccagtt cggcgaatcg ggggatgtcc gagggatgca ggtgctgcgc
     1201 atgctcgatg cgccagcgcc ggtccagcgt gtcggggccg tcgaaagccc gctcgtacag
     1261 gtccagcacc tcgcggttgg cccggtcacc gatcgcgtgg acgttcagct ggaagccgtg
     1321 atcgagcgcg atccgcgccg tccgggcgat cgcggcggga tcctcggtgt tcaggccgct
     1381 ggactccggc ttgtcggcgt acggctggag cagccacgct ccgtgcgagc ccagggcgcc
     1441 gtcgattgac cgcttgatgg atcggaccgt gaggtgatgg tccccgtatc ccaccaggcg
     1501 gtagtcctcg atccgctcct cgagagcgtc gttgcccgcc gagagcatca cgtagaggcg
     1561 cacgggcagg cggccctcgt cggcgagcgt ccggaagagg tccaccgtct cgaaaccgga
     1621 gcccgcgtcg tggaacgacg tgacgccctt cgagagggcc tcctcggcgg caaggcgcgc
     1681 ccgctcgcgg gcctcggcga tggcctcctc cgcggtgcgg ttcatctccg ccgcggcacg
     1741 cgcgcggccc accaggccct gggcattctc gcgcagcagg ccggtcggct ctcctcgggc
     1801 gtcgcggacg atctcgccgc cctccgggct gggcgtttcc gcgctgatgc cgcccaggcg
     1861 catggcctcg gcgttcacga aggtcgcgtg cccggaggcg tggccgagct cgacgggatt
     1921 caggggcgac acggcgctga gcgcgtggtg ggtcggcacg ccgtccacgg agccctcggg
     1981 tgtctcgtcc cacttctcct ggtgccaccc gcgcccaagg atccaggcgc ccggctcggc
     2041 ctcggaagcc gcctcgccca cttggtcgac gatgtcctgc cacgaggtgg cctgcgtgag
     2101 atccaggacc atcttggccc gtccgacgcc catgaagtgc gcgtggccct cgatgaagcc
     2161 ggggacggcg agccggccgc ccaggtccag cacctcggtg ttcgggccga tgagtccggc
     2221 gacgtccgca ctgtcgccga cgacggcgat cgtcgtgccg cgcacggcga gccacgtggc
     2281 ctccgggcgt tcgtcgtcaa gggtgacgat gcgcccgcca tgaaggacca gcgtggcggg
     2341 gttgtcgggg gtggaagtgc cgcaggcggc gaggagcccc gcgaggagca gggcggaggt
     2401 tctggggctc atattccgga tttcagggcg agtatggcgg attcggggtc gtcgacgacg
     2461 gtgaacaggc tgcgggtctc cggcatgaag cccagttcgg tcgcctcttc caggaagtcg
     2521 aacagcggat tgaagtagcg gcggcagttc acgacgacca cgggcttgtc gtggtcgccc
     2581 agctgccgga gcgtcacgat ctccaggaat tcctcgagcg tgcccagccc gccgggcagc
     2641 accacgaatc cgttggcccg cgcgaagatc tcccgcttgc gctcgaagag gtcggcggtg
     2701 acgtgcagct cgtccgccac cgtgtaggcg acgccttcgc gctgccggag cgacgtcggg
     2761 atataccccg ccacgtgccc gccgtgcgcg tgcaccgcgc gggcgagcgc gcccatgaga
     2821 ccgatgtccc cgcccccgta caccaggccg aaatcgttct cggcgagcag gcggccgagc
     2881 gtctcggcct ccctggcgaa ggcctcgggg atgcggcggc tcgcgccgca gaaaactgca
     2941 atgctggcga ccattccgga atcaggcctg gttcgaatga tatatggcca tgctccactc
     3001 ggccgtgcgc agccagccgt tcgaggcgtg ccgccaggcg acgtacgatt ccatgatgcg
     3061 ccggtaggcc gggtcggagg cctcctcgtg catgatctcc tcgctgatgc gcgcggcggc
     3121 ctgcaacacg tcgtccgaga agcggcgcag ctcggtgcct ccggccagca ggcgctccag
     3181 cgctggcggg ttctgcgcat cgtagcgcgc catcatgtcc acgttcgcct ccgcggcggc
     3241 cgcctcgagc gcggcgcggt agtccgaagg cagcccgtcc cacgcggcgc ggttcacgta
     3301 gaacgacagg ttgggcccgg gctcccacca gcccggatag tagtagtacg gcgccacctt
     3361 gtggaagccc agcttctcgt cgtcgtacgg ccccgaccac tctgtggcgt cgatcgcccc
     3421 gcgctcgagc gcggggtaga tgtcgccgcc ggccaggacc tggacggtgg cgccgagccg
     3481 gtccaggacc cgtccgccga agcccgggat gcggatcttg agcccgcgga tgtcggagag
     3541 cccggcgatc tcccgccgga accacccgcc catctgcgtg cccgtgttgc ctcccggaag
     3601 gttcaccacg ttgaagtccg cgaacatgct ccgcgtgagc tccatgccct ccccgtggta
     3661 gagccaggca ttgaactgcc gggcgttcag tccgaacggc acggtgcagt cgaaggccag
     3721 cgcgggattg tgccccacga aatagtagct ggcggagtgg cccatgtgga cggtgccgcg
     3781 gccgaccgag tcgagcacct ggtcgtaggg cacgatctcc ccggccggga acacccggat
     3841 cgtgaaccgc ccgtcggtga gagcctccac gcggcgggag aggacctccg cggccccgta
     3901 gatcgtatcg agggaccgcg ggaagctcga gaccagccgc caggacagcc tgggccgcgt
     3961 ctgcacaccc ggcgccccgc cctctccggg cccgcctgcg caccccgccg cgagcgcccc
     4021 accggccgcg atcgccgagc ccttccggat gaatgctctg cgcttcatgg atcccccgat
     4081 gatgacgccg gaaggttaac cgggacgggc acgccgcgca accgccgccg gcgctatccc
     4141 cgcggccgga gcggccgcat gacgacctcc gagaccaggt agttctccgc ggtcatcagc
     4201 acgtgggata cggtcccggc cacgtcgtcg gcggtcatcg ggttgctcgt gccggaggtt
     4261 cccgcgacct cctggaactc ggtcgcgatc gatcccgggt acacgcaggt caccttgatg
     4321 ccctggtcgc gcacttcctt catcagggcc tcgctgaatc cccggacgcc gaacttcgac
     4381 gcgttgtagg ccgctatccg cgggtttccg atgaggccgg cgacggacgc gatgttcacg
     4441 atgtgccctc cgaagcccgt ctcctcgttc ttcgcccgca tgcccggcag gacggcgcgc
     4501 gtgcacagga aaagcccgcg caggttcgtg ttcatcatca cgtcccagtc gtcgagccgg
     4561 agcgcttcca cctcgccgaa gaggccgagg ccggcattgt tgaccaggat gtccacggac
     4621 acgccgagcc cgcggatggc cgtgaacgcg cgctcgaccg acgcctcgtc ggtcacgtcg
     4681 caggggacgg ggatgaagcg gtcgccgagc tcggtgcgca tcgcctccag gcgatccatg
     4741 cggcgcgcga ggccgatcac ggtggcgccg cgatggacga ggtcccggct gatggctgcg
     4801 ccgagcccgg cggaggcgcc ggtgacgacg gcggtcctgt tttcgagggt catgggtctg
     4861 gagtggaggt ggacgcgtct tacgacgacg gtccgcgagg gtttgggggg cgaagcgtgt
     4921 cggcgccgcc cggcgactcg gccgagcccg taccgcggat gtccggtata agttcgggcg
     4981 tgcgcgtatc ctctgaaagg ctcccggcgt ccgactcacc cccggaacgc atcgccggac
     5041 ccgtaccccc cggaacaagc cctgcccccc atgcttcggc gccccgcaat ccttctgccg
     5101 ttactcttcg ccgcgtccgc cgtggcccag ccggcggagt tcctcccgga gaccctttac
     5161 gccatcggcg gcgcgtcgta cgacgtgcgg agcgtcgacc tgaacggcga cggggcgccc
     5221 gacatcgtct cgtccgccca gggcgacggt ggcgtctcgg tgctgatcaa ccagggtgac
     5281 ggcaccttcg ccccgtcggt cttctacctt gtggcgaccg gtatccgctc gctttcggtc
     5341 ggcgactttg acggagacgg ccaccgggac gtcgtggcat ccgccaacgt gaacacgatc
     5401 tggttcctgc cgggcgcggg agacgggacg ctcggcgccc cggtttccac ggtggacaac
     5461 gacatgtccg gtaacgcgac cgacctggcc gaggccgact tcgacgggga cggaacgctg
     5521 gacgtggtct cgctggccgc aggcacggcc ttcgtcatga ccggcgacgg cgacgggacc
     5581 ttcacgcgga cagatttcgt catgctcgac ggtggccact tcctgtacgg ccgcctcgtg
     5641 gccgaggacc tcgacggcga cgggcacccc gacctcgccg tcgcgaacgg cgactacaag
     5701 gtcctcgtgg cgctcaacga cgggacgggc gatctgggcg ctgcggtggt gatttccgag
     5761 gatattgaag ccttctccgt ggccgccggg gacttcgacg gcgacgggga tcccgacctc
     5821 gtggcggccg gcatgggatg gatacagacc tttgccaacg acggcagcgg agcgttcgtg
     5881 ctgtcggggg aggcgaacgc gagcggctcg tcgctcggca ccgcggtcgc cacggccgac
     5941 ctgaacctgg acgggcactt ggacgtcgtg gtaacggccg gctcggtcgg gctcaacccg
     6001 tattacggtg cgggcgacgg gagcttcgct gcgggcgcga cgatcgcgac gtcgctcgac
     6061 aaccacttcg gcatgctcgc ggacgatctc agcggcgacg gcaagccgga cctcgccgcg
     6121 accgacacgg gcgtgctcga gaacgcattg gtggtgcgtc tgaacgcgac cgaacccgcg
     6181 tcgtccgccg tggccgccga cgatcactac gtcaccgccg tgggacaggc cctgtccatc
     6241 ccggcccccg gggtactggc gaacgacgcg ggtgcgccac cgctgcaggc ctttcctctc
     6301 ggttttcctt ccgccggttc cgcgtccctg tcgctcgacg gatcgctcgc ttacatcccg
     6361 gatccggggt acgagggaac ggtcagcttc acgtacgagc tcaccgacgg cggcgggcag
     6421 acggaccagg cgacggtttc gatcaccgtt acgtccgtcg aaccgctgct catggccacg
     6481 agcatcgaca tcgctcccat ggacccctgg gtaacccgcc ggcgcacggg tgaaccgaac
     6541 atcgaccacc tggcggagct cgccgcgggc ggcggggggg aggtgctcct caacttcttc
     6601 ggtgacgtcc aggtgctcgc ctaccaggac ggcgtcgagt ggcgggacga ggcccggttc
     6661 acgtggttcg ggcgccgggt cgggaacgag cgggatcacg tgatcctggt ggtggacctc
     6721 aacctgggca ccgtcaccgg caatgcgacc gtggacgggc gcatctacca ggtgcggccg
     6781 gtgagcacct ggcatgaggc ccgggaaatc gaccaggccg gcttcccgga ggaggaagac
     6841 gacgcgccgg aggacgagga agacatcgcg ggcaagaccg gcggtggcgc cgcatggccg
     6901 ggggcgctgc tcgagcccgg caacagccac ccgatcatcg acatcctggt cgtgtacacg
     6961 gacgatacgg ccgccgcatc gccggacatc ctctcggaaa tccagctcgc cttcgacgag
     7021 accaacctcg gtttcgccca gagcgggata aacgcggagg tccggctggt cgcgaccgag
     7081 ctcgtgaact acgaggaaac ggggaacgcc accacggacc ggacccgcat caagcggacc
     7141 tccgacgggc atctggacca cgtgcacgcg ctcagggacc aggtcggagc ggacgtcgtg
     7201 agcttctggg tggaggacgg cggcagcagc aagtgcggcg tggcctacat catgaagcag
     7261 gtcgacggct cgttccgcga ccgggccttc ttctccgtgg tgcgaggctg cgcgacgggc
     7321 aacttcactt tcggccacga gctcgggcac gtcatgggcg cgcgccacga cggcttcgtc
     7381 gatcccttca acgtgccgta tccgtacagc catggctggc tggacgtcgg cgaccgctgg
     7441 cgcaccgtca tgtcgtacaa cgacgcctgt gactgctccg acgagcccgt gcagccctgc
     7501 ccgctcaagg ccttccggtc cacgaagaat gcgccggtct gcagccgggt cctcttctgg
     7561 tcggaccccg acgggtcctt cgacaatccg aaccgtgcgg atccgggggg cgcgatgggt
     7621 ctcgcggaca aggccgacaa cgtccgcgcg ctgaacaaca cggcagccac cgttgccgcc
     7681 tattccgacg cggttttcgc cctggtccgc ggacgggtta cgcaccaggt ttcgggggac
     7741 agcctggaag gcatccagat cacgggcttc ccctccccgg tggtgaccga caccacgggc
     7801 ttctacgaga ccctggtgcc gctccactgg tcgggtaccc tcgaggcgcg caacgacacg
     7861 ctggcgttcg ttccgccctc ggcctacatc gccgacgcga cgggaaacat cgagcaggac
     7921 ttcgagggca tcgagcggac cgtgtccgga cacgtctgga tcgagaacac ctccgacttc
     7981 gtgcagggcg tcgtgatcga gggtttcgac gagccggtcg aaacggacgc cgacgggcat
     8041 tacgagatca tcgtgcccca cgaatggtcg ggcgtgctcg tgccgaagct cgagccgtac
     8101 cggttcgacc cgccgtccgt cgctatttca tcggtgatcg ccccgacgat gcaggacttc
     8161 gtcgccctgc tgcccccgat cgtctccggc accgtcacgg aatccggaac gggggcggcg
     8221 atcgagggcg cctggctctc gggctgtccg gtcgaggtcc tgacggacgc ggcgggggcg
     8281 tacgcctgtc cggtggaatt cggctggtcc ggaacgctcg tggcgctcgc cgccggctac
     8341 aacatgacgc cggagacccg ctcgtacgag gccgtgtggg acgacctctc tgggcaggac
     8401 ttcgtcggcg agccgggcgt gctggcgaac tcggactggc cgctcacggg gcgcgaccag
     8461 gcacggaccg cgggcggcac cgggtcgccg gtgcggtacg ggacccggtg gacgtacgag
     8521 gacggatccc gctggccgtc gtcccccgtc gtcggcccgg gtgggctggt atacttcggc
     8581 accgcgaacg gacgcctgcg cgcgctcgac cccaccggca gtccccggtg gctcgcgttg
     8641 ccgtcgtcgc caccggacgc gggcggactt gcggtcgggg cgggaggcgt gctctacatg
     8701 acgcagtcct ccctcgccca cctgtcctcc ggcacggtga gcgctgtgct tccggacggc
     8761 agcggcaccc tgtggagcac ctcgttccag gaacgcgtcg acgtcgcacc gaacctcggc
     8821 gcttccggca cgctctacgt gggcacgagc cagggcgacc tcgtggcgta cgacgcttcc
     8881 gacggcaccg agcgctggag gttcgagacg gagggcggcg ccgtctcgtc tgccgcgctg
     8941 accggcgacg agacgatcgt cgtggggacc caggcgggtc gcgtgtacgc cgtgaatccc
     9001 gacggcacgc tgggctggat gcatgacacg ggcttcacta gctcctcggc cccggccctg
     9061 gccccggacg ggacggtgta catcgggtcg gacgaccaca ggctctacgc cgtcggcgcg
     9121 gacggcgcgc tgaagtggtc gttcgcgacc ttgggaccgg tgcgcgcgac gccggcgctc
     9181 ggccccgggg gagaggtcta cgtgggatcg gacgacggcc acctgtatgc gatctcatcg
     9241 ggcggacagc tgcgctggaa gtaccggacc ggcggtcccg tcacggcggg ggcggtggtg
     9301 agcacgcacg tgtccccgtt cgatccgctc ctgcccggcg tggagcagac cgagacggtc
     9361 gtgtatttcg gctcgggcga cgggacggtg tatgccgtta acgcgggggg cacgttcacg
     9421 gcgagcatgc ccggcaccct ccggtggagc actgcgaccg accatccgat cgagtacgcc
     9481 cccgccctgg acttcgccag gcagaccctg tacgtgagct ccggcccccg cctctgggcc
     9541 ttcggcgggc agccgtccgg cgggacgggc ctcgtccaga tcacgagcct ggggacggtc
     9601 ctggaccccg agatcctcga gctcagggac aacatccaca tcctcgtcgc gctagccgga
     9661 gagggcggct tcggcggcag cgccatcctg ggggggcttc cggaggatcc ctgccagggc
     9721 ggaccgcttt tcatcccgga ctgccaggac ctcgcgtcag gcctcggcac ggccacgccc
     9781 ctcgtcgagg tgccctcgct cgtcgacctg cagatcgggt tcgtgaagga gggcttcggg
     9841 caggccgtgc tcgggggcga ccccgacggg ctcctgtcga cctacacgac cgtgctccgt
     9901 gacggcggaa cgtacacggg gatcctggcc ggcaccacgc atccggagct gctcgccccg
     9961 aaccccgacg ggctgcccac ccggctgacc gtgttcgagg cgcccacggg gaccggttcc
    10021 gggacgggcg agcccgtcta cgtcggccac ttctcgacgg acctcgggac ggtcgagatc
    10081 aggctcacgg gcagcgggac gtacacgttc gggcccctgc gctacggcga gctctccgac
    10141 ctcgccctcg tggagcccgg cgcgtacgag atccaggtcg tgccgctgga cggggtcgcg
    10201 ggcaaacggc aggccgcgct cgtcgcgtcc ctggacctgg cggccggcgg gccgaccggc
    10261 atcctgctcg ccggattcgc cgatcccgag gccaacctcg gcgggcccgg gctcacgatg
    10321 taccgggccg acggcggcgg cgggaccgac gtcatcggcg tggcggtcgg gccccggttc
    10381 gaactcccgc ggcacgtgac gctcgagccg gcctacccga acccgttcaa cccggtcacg
    10441 gtgatcgcgt acgcgcttcc cgacgagcgt gacgtgcgcc tggaggtctt cgacgtgctc
    10501 ggccgcagcg tggcacggtt ggtggacggc cggaaggcgg ccgggcgcca cgaggtatcg
    10561 ttcgacgcgg gccggctggc gggcggcgtg tacctggtcc ggctcatggc cggggggcag
    10621 gtcgcgaccc gcgccgtgac gctcctcaag taggtccggc gggggtatct tggcgcggcc
    10681 accccctgcg cgatgactcc cgtcgaatcc ggaaaggacg ctgttgaccg cctgctggcc
    10741 gaggcccggc cggcgtcgga cgggttcacg gcggccggac ggcggctgga gccggacgac
    10801 gtggtccggc ggctcgcgcc gttcctgagc ggggagcgcc ggcggcgcat cgagtccgtg
    10861 ctcgacgggc gcacgtacgc cgtggccacc gtggtggagg ggctttcgaa cagcggaaac
    10921 gtgagcgcgg tgatgcgcac ggccgaggcc ctcggcttcc agcggttcga cctcgtgacc
    10981 ggcagcgcgc ccttcaagca ctcgcggcgg accacgcagg gtgcccagaa gtggctggac
    11041 gtgcacgtgt ggccggaccc cgacgcgtgc gtgggcgcgc tcaaggccga ggggtaccgc
    11101 gtggtggcgg cgcacctggg cgcgggagcc gagcccgtcg acggggtcga cttcaccgtg
    11161 cgtacggccc tcgtcttcgg caacgagctc tcgggcgtgt cggagcggat gctgcagctg
    11221 gcggacgcgc gctgcatcct gccgatcacc ggcttcgcgc agagctacaa catctcggtg
    11281 gcggccggca tggcgctcta ccgggcctgg caggaccgca cggcgcgcct ggggcggtgc
    11341 ggcgacctgg cggccgggga ccgcgagcgg ctgcgcgcgg agttccacgt gcgcgcgctc
    11401 cgcaacgccc gggccatcct ggaacgctcg atccgcgagg aggccgccga tggctgactg
    11461 gtggcctccg ggagctctcg ccgggcgggt cctggacacg ccggtcggcc ccgtgacgat
    11521 cgcggcgacg gccggaggcg tcgcaggcat ccggttcggg cgcgtggcca tgccgccgga
    11581 ccccggcgcg cgggcaccgg cctcggcgag gaccgccgtc gggatggtgg agcgggcgtc
    11641 ccgccagctc atggcgtact tcgatgaggg cagcacgggc ttcgacgtgc ctctcgaccg
    11701 gaccgggact ccgtttcagc tgcgcgtgtg ggaggccatc gcgtcgattc cgtacggggc
    11761 gacggcaacg tacgggcagg tcgcgcggcg gctgggcgac gtccggctcg cgcgggcggt
    11821 cggggcggcg tcgggcgcca accccgtgcc catcatcgtg ccgtgccata ggctcgtcgg
    11881 ttcggacggc agcctcacgg ggttcggcgg cggcctcgac gtcaaggccc tgctgctgcg
    11941 ccacgaggcg cgggccgtgc agccggatct gttcggcgga ccctgagacg ggctcggccg
    12001 ttcggggacc ggtcccttag cgagcccgcc catgcgccga ttcctgccgc tttccgtcct
    12061 cctggccctg acggggtgcg gaggctccgc cccggtcgtc gagcccccgc cgcccgcgcc
    12121 cgtcgtcgag cccccgccgg ggcccgcgtt cgtcgtcgcc gtcgtcgagc ggggacaggt
    12181 ggccgtccac gatctgcgga ccggcgcccg gcggacggtc ggcggcttcg ggggcgaggt
    12241 ggcgtcccgg cacgcggcgc cggccgggtc gtcgtcggtc gtggcgatcg ccgacgccga
    12301 ctcggtgcgg ctggtccatt cggacccggc ccgcggaacg ctgcgggtcg ttgcctcccg
    12361 gcccgtcccc gtgaggtaca cggtggcctg ggcctcggat ggcagcgcct tcgcgtacgg
    12421 gtacta
//
LOCUS       DBNHQZ010000370         8217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_110801, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000370 DBNHQZ010000000
VERSION     DBNHQZ010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_110801"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="AC16YU_10030"
     CDS             complement(<1..825)
                     /locus_tag="AC16YU_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065607.1"
                     /translation="MKTELLIPGPTFIPSGSHVDCRAVTAFAGRRHRPAGFLFGALLL
                     AVLTAGTITAQPARKAVRATVSVPDVNSPITDLRSLDLDALDADPRLRRAGMRSLSDL
                     RDWHVLRASAGSVSTSGSSVASKSGAAGFAAMQEFEGPVAGTPYDFPALVTLADGDIL
                     MAFRDPGVFPEFWDGPPTDPAKVLLARSEDGGATWGEAVQLGEGDFPLDFFNLELTRL
                     GSGRLLLNDLTPFEEDHYWISDDDGATWTATPITERLSPGWHGPIDGKVWYTYLSDA"
     gene            complement(951..4925)
                     /locus_tag="AC16YU_10035"
     CDS             complement(951..4925)
                     /locus_tag="AC16YU_10035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator transcription factor"
                     /protein_id="HIR6065608.1"
                     /translation="MRRPDTSGFVRHGARWVCLAALCAASVHAAAAQPAAEPGRPYFR
                     NFAPAEYDAHVWNSSAIEDRRGVLYVGNNDGLLEYDGVSWRLIVLPGRSHVSSLGRDD
                     EGRIWVGGFGDMGFLAPDPVGRATFVSILPRVPAGATGFTFVEQTVPAADGIYFATRS
                     SVLRYRPDPADPAEGRVERLLKNPEGVDLFPVDGQLLVWQPGIGLERIVDGRTEPLPH
                     GSRFASEPVRFVLGRRDGSILVGTARSGLFLVDREGAVRFGGSSTGVRAGDRVTSGVA
                     LSGGRYAVGTEAGGVLVIDGTGRLLYRIDSSTGLRHDAVNGLYADAAGSLWALLDMGL
                     ARIDVDTPLTVFDGVLGLDEFVMTVHRHGGRLYAGTLSGLSVLDPGTGRFGPVPGVSS
                     RTVGLHPVGEDLLAATASGVAVVRDDSPGATIPLDLGGSGAYLLAGSAPDPDVVFVGY
                     EGGIRLARREGGSWRDLGRVEGVDFPVHSGVGLADGTVWIVDEEGTAFRLRPLAHPIL
                     RPADVEARPFGPAEGLPPVRATVYEVGDRPYFTSFGAAVWTVNEAGDRLVRTSRFDAL
                     RTDHPLDWFGFNEDGQGRVWTPLGNRGMSRADPMPDGTYRANPGPLRRLGPLLVMQAF
                     PDADGVVWMASSDGVVRYDANLQPGPAGGHPALIRRVVARDDSLLYEGFGPPRAIEMA
                     HARNSVRIEYALPSLGGSSEYRTLLEGFDEDWSPWSRETDRTYTNLPEGDYTFRVLAR
                     DVDGMESEEGRLSFTILPPWHRTAWAYLLFAALGLGAIFLLRRYEMGRIRLKYRLQLQ
                     HVESERLKDLDRVRSDFFANISHEFRTPLTLILGPVSDALEERRDLSRDQLGVLHRNA
                     QRLLRLINQILDLSKLEAGAMTLRASREDIIPFLSGLLYSFESVAERRGIGLHLEAPP
                     GRLYVSFERDRLEQVFLNLFSNAIKFTPDGGSITVTCGRSEDGLAEIAVGDTGCGIPE
                     DRLEHIFDRYFQSGLSPAREKEGTGIGLALTRALVELHGGRITVTSRMDVGTTFTVHL
                     PLAGDEDPAEDGDRVSVSGDGAALPAVDDFEEPREPSAPGVEDGRPIVLVVEDHPDVR
                     SYLRECLAETYDVREAVDGADGLERARALVPDLVVMDVTMPRLDGYGLSRALRADERT
                     SHVPIVMLTARAAQADRIEGLETGVDDYLLKPFNPQELRLRVRNLIAQREAARRRFST
                     ATVIRASEVAATSIDRAFLERVVAIIEERLADEQFTVDRLATEVGMSVSQLNRKLNAL
                     IDQAGGSLLRSMRLQRAADLLAQRAGSVSEIAYGVGFSTPAHFSTAFKKQFGVPPSAY
                     RGES"
     gene            complement(5083..7191)
                     /locus_tag="AC16YU_10040"
     CDS             complement(5083..7191)
                     /locus_tag="AC16YU_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6065609.1"
                     /translation="MWYRSRLVALLAPTLFIGTIAHAQPTAQFAWRALNGPFGGTAPV
                     ILEASDGTLFAGSGGNEGGVYRSLDGGLTWQRSSAGIPLADRSVDGIVEADSGHILVG
                     TNSHIGSRFYRSTDGGETWTEQAILGCLAFTKTPEGHLLAANTGYGAIRRSTDNGYTW
                     EEVDVPFGWTDDIAVNDTGHVFVGGWGFLRSRDGGATWDPVAGPGGNVRSLAIDDEGR
                     VYAGNWGEYASNSGVFRSDDNGETWAPLRLGFRVNRNRNITFTPEGHILVGSWEQGIW
                     KSTDDGATWYQANTGLGHLNLRHLHVTADGTVLAGTEGGGTYRSADGGETWTQVGLTA
                     ARATRLAFSPATGDLFALVVGMARSSDRGESWVPVNSGLTNFDLRSFDIAPDGTLYAG
                     GAGLVFRSDDNGDTWTPASSGIENHTVEAVGVAPDGTVYAANYYGIFESEDRGASWTK
                     VDGFSGVRWIDFNEEGDLFAASWGQGMKRRRYGQETWEVLDVGYPWVSPLYVGRNGFV
                     YASTGRSEDNGDTWTAMGYGSSVAGYVENGIGQLFAATYNYGNGVHRSTSYGVTWEKV
                     NAGIPSNDARAITVDSDDYLYVAPYGHSVYKSVLPTTVSTGVEDDSTPPGSFRLDQNY
                     PNPFNPATTFRFTLPGRSFARLAVVDPLGREVAVPVSEILEAGEHVRTWDPRGLASGV
                     YVYILQAGPWRQARTLALVR"
     gene            complement(7388..8074)
                     /locus_tag="AC16YU_10045"
     CDS             complement(7388..8074)
                     /locus_tag="AC16YU_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065610.1"
                     /translation="MKSTLLCALSLIASPVLAQTSSGTLGGGDAQRGDGVFYDAVTLQ
                     VDVPSEITVRMESGDFDTYLIVRSPSGQETINDDYESQSVSQVDVIATETGPWTIWAS
                     SYGSGMEGAYTLSVTRGAELEIETVEGRLDYRDPVALKGEFFDTLERDLPVDGTYIFE
                     LLSLGYDGYLVVTSPSGEVWRNDDAGSTRVSRVGPVRGSAGRWTIQVTSAVAGEQGAY
                     DLNVVRVLEN"
BASE COUNT         1315 a         2920 c         2779 g         1203 t
ORIGIN      
        1 ggcgtccgag aggtacgtgt accagacctt cccgtcgatg ggcccgtgcc agccggggct
       61 gaggcgctcc gtgatcgggg tggccgtcca cgtggccccg tcgtcgtccg aaatccagta
      121 gtggtcctcc tcgaacggcg tcaggtcgtt aaggagcaga cgtccggatc ccaggcgcgt
      181 cagttccagg ttgaagaagt cgagcggaaa gtcgccctct ccgagctgga cggcttcgcc
      241 ccacgtggcc ccgccatcct cgctccgcgc gaggagaacc ttcgccgggt ccgtcggcgg
      301 gccgtcccag aattccggga agacgcccgg atccctgaag gccatgagga tgtcgccgtc
      361 ggccagggtc acgagcgccg gaaagtcgta cggcgtccct gcgaccgggc cctcgaactc
      421 ctgcatggcg gcgaagcccg cggcgcccga cttcgaggcc acggacgaac cgctcgtcga
      481 cacggatccg gcgctcgcac gaagcacgtg ccagtcccgg aggtcggaga ggctccgcat
      541 tcccgcgcgc ctcagcctcg gatcggcgtc cagcgcgtcc aggtccagcg aacgcaggtc
      601 cgtgatcggg ctgttcacgt cggggaccga aaccgtcgcc cggaccgcct tgcgcgcggg
      661 ctgggccgtg atcgtgcccg cggtcaggac ggcgagaagg agggcaccga acaggaatcc
      721 ggccggcctg tgccgccggc cggcgaacgc cgtgacggcg cgacaatcca cgtgggaccc
      781 ggaaggaatg aatgtcgggc ccggtatcag tagttcagtt ttcatggctc tctctggcgt
      841 gttgatggtt ctccgactgc gtgaaccaaa cgtaacggga gagcgccgcg tcgtgctttc
      901 ggattccgcg caaaggcttt cgaagcctgc gcaaacggcc gtccgagccc tcaggactcg
      961 ccccggtacg cggacggagg cacgccgaac tgcttcttga aggccgtgga gaagtgggcc
     1021 ggggtcgaga agcccacgcc gtaggcgatc tccgacaccg agccggcgcg ctgcgccagg
     1081 aggtcggccg cccgctgcag ccgcatggac cggagcagtg agccgcccgc ctggtcgatc
     1141 agcgcgttca gcttgcggtt gagctgggag acgctcatcc cgacttcggt ggcgaggcgg
     1201 tccaccgtga actgctcgtc ggcgagccgc tcctcgatga tcgcgacgac gcgctcgagg
     1261 aaggcccgat cgatgctggt cgcggccact tccgatgccc gtatgacggt ggctgtgctg
     1321 aaccttcgcc gggccgcctc gcgctgcgcg atcaggttcc ggactcgcag cctcagttcc
     1381 tgcgggttga acggcttgag caggtagtca tcgacacccg tctcgagccc ctcgatccgg
     1441 tccgcctgcg cggcgcgcgc ggtgagcatc acgatgggca cgtggctggt gcgctcgtcc
     1501 gcgcgcagcg cacggctgag cccgtagccg tccagccggg gcatcgtgac gtccatgacc
     1561 acgaggtccg ggaccagcgc ccgggcccgc tccagcccgt cggcgccgtc gaccgcctcg
     1621 cggacgtcgt acgtttcggc gagacactcc cgcaagtagc tccgcacgtc cggatggtcc
     1681 tcgaccacga ggacgatggg cctgccgtcc tcgacgcccg gcgccgaggg ctcgcgcggc
     1741 tcctcgaaat cgtccaccgc cggcagcgcg gctccgtcgc ccgagacgga cacccgatcc
     1801 ccgtcctcgg ccggatcctc gtcgccggcc aggggcaggt gaacggtgaa cgtcgtgccg
     1861 acgtccatcc ggctcgtgac cgtgatccgg ccgccgtgca gctccacgag ggccctcgtg
     1921 agcgcgagcc cgatccccgt gccctccttc tcccgtgcgg gcgagagccc ggactggaag
     1981 tagcggtcga agatgtgctc gagccgatcc tccggtatgc cgcagccggt gtcgccgacg
     2041 gcgatctccg cgaggccgtc ctccgagcgg ccgcacgtca ccgtgatcga cccgccgtcc
     2101 ggggtgaact tgatcgcgtt cgagaacagg ttcagaaaga cctgctccag gcgatcccgc
     2161 tcgaacgaaa cgtacagcct gcccggcggc gcctcgaggt gcaggccgat cccccgccgc
     2221 tcggccacgg actcgaagga gtagagcagc ccgctcagga acgggatgat gtcctcgcgc
     2281 gaggcgcgca gcgtcatcgc cccggcctct agcttcgaaa gatccaggat ctggttgatc
     2341 aggcgcagga gccgctgcgc gttccggtgc aggaccccga gctgatcgcg cgacaggtcc
     2401 cgccgctcct cgagcgcgtc cgagacgggt ccgaggatga gcgtgagggg cgtgcggaat
     2461 tcgtgactga tgttcgcgaa gaagtcggat cgcacccggt ccaggtcctt gagtcgctcg
     2521 gactcgacgt gctggagctg cagcctgtat ttcaagcgga tccggcccat ctcgtaccgg
     2581 cggagcagga atatcgcccc gagtccgagg gcggcgaaca gcaggtatgc ccaggccgtg
     2641 cggtgccagg gagggaggat ggtgaacgag aggcgtccct cctcggactc catcccgtcg
     2701 acgtcccggg ccaggacgcg aaacgtgtag tcgccctcgg gaagattggt gtacgtccga
     2761 tcggtttccc gggaccaggg cgaccagtcc tcgtcgaaac cctcgagcag ggtcctgtac
     2821 tcgctcgagc cgccgaggct cggcagcgca tactcgatcc ggaccgagtt ccgggcgtgg
     2881 gccatttcga tcgcacgggg tggaccgaat ccctcgtaca gcagcgaatc gtcgcgcgcc
     2941 acgacgcgcc ggatgagtgc cggatggccg cccgccgggc cgggctgcag gttcgcgtca
     3001 tagcggacga cgccgtcgga actcgccatc cagaccaccc cgtctgcgtc cggaaaggcc
     3061 tgcatgacga gcaggggacc gaggcggcgg agcgggcccg ggttggcccg gtacgtgccg
     3121 tcgggcatcg gatccgcccg tgacatgccc cggttgccga ggggcgtcca cacccttccc
     3181 tgcccgtcct cgttgaatcc gaaccagtcc aggggatggt ccgtgcgcag tgcgtcgaac
     3241 cgggaggtgc gcaccaggcg gtctcccgcc tcgttcaccg tccagacggc tgctccgaac
     3301 gaggtgaagt acggcctgtc gcccacctcg tacacggttg cccggacagg aggaaggcct
     3361 tcggcgggcc cgaacggccg cgcctcgaca tcggccgggc ggaggatcgg gtgcgcgaga
     3421 ggccgcagcc ggaaggccgt gccctcctcg tccacgatcc atacggtacc gtctgcgagg
     3481 ccgaccccgc tgtgcaccgg gaaatcgact ccctcgaccc ggcccaggtc gcgccacgac
     3541 ccgccttccc gacgagccag gcgaatcccg ccctcgtagc ccacgaagac gacgtcgggg
     3601 tccggcgccg atccggcaag caggtaggcc ccggatccgc cgagatccag tggaatcgtg
     3661 gcacccgggg agtcgtcccg cacgacggcg accccggaag ccgtggcggc caggagatcc
     3721 tcaccgaccg gatgaaggcc gacggtccgc gacgaaacgc ccggcaccgg accgaacctg
     3781 cccgtacccg ggtcgagcac gctcaggccg gagagcgtgc cggcgtacag gcgccctccg
     3841 tgccggtgga ccgtcatcac gaactcgtcc agcccgagca ccccgtcgaa gaccgtgagc
     3901 ggagtatcca cgtcgatccg ggccaggccc atgtcgagca gagcccacag gctgccggcg
     3961 gcatcggcgt agagaccgtt caccgcgtcg tgccgcaagc cggtcgacga gtcgatgcga
     4021 tacaggagac ggcccgtccc gtcgatcacg agcacaccgc ccgcctccgt tccgactgcg
     4081 taccgccccc ccgacaaggc cacgccggag gtcacgcggt cgccagcccg caccccggtc
     4141 gaggagccgc cgaaccggac agccccctcg cgatccacga ggaagagccc ggaacgggcc
     4201 gtgccgacca agatcgatcc gtctctgcgc ccgaggacga aacggaccgg ctcggatgcg
     4261 aagcgcgatc catgcgggag gggctcggtc cgcccgtcga cgatacgctc gaggccgata
     4321 ccgggctgcc acaccaggag ctgcccgtcg accggaaaga ggtcgactcc ctccgggttc
     4381 ttcagcagcc gctccacccg gccctcggcc ggatcggcgg ggtcgggccg gtagcggagg
     4441 acggacgacc gcgtcgcgaa gtagatcccg tcggccgcgg gaaccgtctg ctcgacgaac
     4501 gtgaagccgg tcgctccggc aggaacccgc ggcaggatcg agacgaacgt cgcccggccg
     4561 accgggtccg gggcgaggaa acccatgtcg ccgaagccgc ccacccagat ccggccttcg
     4621 tcatcccggc cgagcgagct gacgtggctg cggccgggca ggacgatgag cctccagctc
     4681 actccgtcgt attccagcag gccgtcgtta ttcccgacgt agagcacccc gcgccggtcc
     4741 tcgatggcgg acgagttcca gacgtgggcg tcgtactcgg ccggggcgaa attcctgaag
     4801 taggggcgtc cgggctcggc ggcgggctgg gcggccgcgg catggaccga tgcggcgcag
     4861 agcgctgcca gacagaccca ccgcgccccg tggcgcacga atccgctcgt gtcggggcga
     4921 cgcatcgaag ggggttctgc tgccgtcagg ttcaagtggc cggcccaccg ccgggggcgg
     4981 caaggtacgg cacgcgggcg ccggatcgag gattcgccca gccgcggccg ccgccccggg
     5041 accgggcgtc cgcgcggcgt gcgcccgtga ccggccgtgc gctcaccgga ccagcgccag
     5101 cgtcctcgcc tgccgccagg gaccggcctg gaggatgtac acgtacacgc cgctcgcgag
     5161 gccgcgcggg tcccaggtcc ggacgtgctc gccggcttcg aggatctccg agactggaac
     5221 ggccacctca cgcccgagcg ggtcgacgac cgcgagccgc gcgaaggacc ggcccggcag
     5281 cgtgaaccgg aaagtggtcg ccggattgaa cgggttcggg tagttctggt cgagccggaa
     5341 gctccccggc ggcgtcgagt cgtcctctac ccccgtcgag accgtggtgg gaaggaccga
     5401 cttgtacacc gagtggccgt agggcgccac gtacaggtaa tcgtcggaat cgaccgtgat
     5461 ggcacgggca tcgttcgacg ggatgcccgc attcaccttc tcccaggtga cgccgtagct
     5521 ggtcgaacga tggaccccgt tgccgtaatt gtaagtggcg gcaaagagct gcccgatgcc
     5581 gttctcgacg tacccggcca cgctgctgcc gtatcccatc gcggtccagg tatcgccatt
     5641 gtcctcggac cgccccgtac tggcgtagac gaagccgttc ctccccacat agaggggcga
     5701 gacccacgga tacccgacgt cgaggacctc ccacgtctcc tggccgtacc gcctgcgctt
     5761 catcccctgc ccccagctcg ccgcgaagag gtcgccctct tcgttgaagt cgatccagcg
     5821 cacgcccgag aatccgtcta ccttcgtcca cgaggcgccc cggtcctcgg actcgaagat
     5881 cccgtagtag ttggccgcgt agacggtgcc gtcgggcgcc acccccaccg cctcgaccgt
     5941 gtggttctcg atgccggagc tcgccggcgt ccaggtgtcc ccgttgtcgt ccgatcggaa
     6001 cacgaggccc gcgcccccgg cgtagagtgt cccgtcgggc gcgatgtcga acgagcgcag
     6061 gtcgaagttc gtgagcccgc tgttgacagg cacccaggac tcccctcggt cggaggaccg
     6121 ggccatgccg acgaccaggg cgaagaggtc gcccgtcgcc ggactgaagg cgagcctggt
     6181 cgcccgcgca gccgtgaggc ccacctgcgt ccaggtctcc ccgccgtccg ccgaacggta
     6241 cgtgccgccg ccctccgtcc cggcgaggac ggtcccgtca gccgtcacgt gcaggtggcg
     6301 aaggttcagg tggccgagcc cggtgttggc ctggtaccac gtcgcgccgt catccgtgga
     6361 cttccagatg ccctgctccc agctgccgac caggatgtgc ccttccggcg tgaaggtgat
     6421 gttgcggttc cggttgaccc ggaatccgag cctgaggggc gcccacgtct ccccgttgtc
     6481 gtcggaccgg aatacgcccg aattcgacgc gtactcaccc cagttcccgg catagacgcg
     6541 cccttcgtcg tcgatggcca gggatcggac gttgccgccc ggacccgcga ccgggtccca
     6601 ggtcgccccg ccgtcccgtg accggaggaa gccccacccg ccgacgaaga cgtgtcccgt
     6661 gtcgttgacc gcgatgtcgt ccgtccagcc gaacggcaca tcgacctcct cccacgtgta
     6721 cccgttgtcg gtcgaacggc gtatcgctcc gtacccggtg ttggccgcca gaaggtgccc
     6781 ttcgggcgtc ttcgtgaagg cgaggcaccc caggatcgcc tgctcggtcc aggtctcgcc
     6841 cccgtcggtc gaccggtaga accggctccc gatgtggctg ttggtgccga cgagaatgtg
     6901 gccactgtct gcctcgacga tgccgtcaac ggagcggtcg gccagcggaa tcccggcgct
     6961 ggaacgctgc cacgtgaggc cgccgtcgag cgaacggtag accccgccct cgttcccccc
     7021 cgaacccgcg aacagggtgc cgtccgaggc ctccaggatg accggggccg tgcctccgaa
     7081 cggcccgttg agcgccctcc aggcgaactg ggccgtgggt tgggcgtggg cgatcgtacc
     7141 gatgaagaga gtcggggcaa gaagagcgac gagccgcgaa cggtaccaca tgaccggaac
     7201 ctccgtagac ggtgactctg gacggacacc cccttcccat ccggacagac gctacagaat
     7261 ccggtcggcg gagacacgta gggaccgtcc ccggccgcgc cacggacaac tccctacggg
     7321 atggagcctt tggagacagg ccgaccacac gcacgcgtgg cggcgccccc cgctccgggc
     7381 ctgcgactca gttctcgagc acgcgcacca cgttcaggtc gtacgctccc tgctctcccg
     7441 ccacggcgct cgtgacctgg atggtccacc ggcccgccga gccccgtacc gggcccaccc
     7501 gtgacacgcg cgtcgacccc gcgtcgtcgt ttcgccagac ctcgcccgag ggcgacgtca
     7561 cgaccaggta tccgtcgtat ccgagcgaga gcagctcgaa gatgtaggta ccgtcgacgg
     7621 gcaggtcgcg ctcgagcgtg tcgaaaaatt cccccttcag cgccacgggg tcccggtagt
     7681 ccaggcggcc ctcgaccgtc tcgatctcga gctcggcgcc ccgcgtgacg gaaagcgtgt
     7741 atgctccctc cattccggaa ccataggagg atgcccagat cgtccacggc ccggtctcgg
     7801 tggcaatcac gtccacctgg gaaacggact ggctctcgta gtcgtcgttg atcgtctcct
     7861 gtccggacgg cgagcggacg atcaggtacg tgtcgaaatc gccggactcc atccgcacgg
     7921 tgatctcgga gggcacgtcg acctggagcg tcacggcgtc gtagaagacg ccgtccccgc
     7981 gctgcgcgtc cccgccgccg agcgtgccgc tcgatgtctg ggcgagaacg ggagatgcga
     8041 tcagcgacag cgcgcagagg agagtggact tcatggatcg gctggatcgg atgggagggg
     8101 atcggtccgg atcgaaaccg cccccggaga ccgtggttaa cggaccggcg cttgaacgta
     8161 cgcttgcgca aaaacgatcc gccttcgggc gacgtgacac ccatcgatcc agtctcc
//
LOCUS       DBNHQZ010000371         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_111529, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000371 DBNHQZ010000000
VERSION     DBNHQZ010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_111529"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..517)
                     /locus_tag="AC16YU_10050"
     CDS             complement(17..517)
                     /locus_tag="AC16YU_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6065611.1"
                     /translation="MNGHVLVLNQDYRALTVCSVERATILVLLQKAELVEAEPDRWLR
                     SPSRRVPWPSIVRLRAYVTIPFKRITLTRKNVMRRDRNRCQYCGNRDKLTIDHVMPRS
                     RGGRDSWENLVTACVPCNNRKGSRTPDEAGMPLRRRPFRPSHVMFIRDFVGTLDDTWK
                     PYLFLS"
     gene            complement(693..1517)
                     /locus_tag="AC16YU_10055"
     CDS             complement(693..1517)
                     /locus_tag="AC16YU_10055"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6065612.1"
                     /translation="MRLESIRIYPVKSGGEVRLDRAQVQAAGLRHDRRWMVVEPDGRF
                     VTRRQEPALAGLRVADAFDAWELRHGAGDGCILPKSWAAGRPMDVEIWGQHVRALEAR
                     DDVSAWLSERLGRALALVFVPEADLRPVEPEFARPGDVVGFADAYPALLVNAASVRAL
                     ARVVGRPLEDRRFRPNLVMSGAEAFAEDGWASIRIGSVTFDVAKPCARCRVIDQDPGT
                     GKPDGGVLRALGTFRRSGRKVLFGENLVPRSAGEVRVGDAIEVLARRAEASVIRDA"
     gene            complement(1522..>1719)
                     /locus_tag="AC16YU_10060"
     CDS             complement(1522..>1719)
                     /locus_tag="AC16YU_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6065613.1"
                     /translation="QSDHGAFHRAGIPFVYFGVEDHEDYHRPSDDTERIDADFFARAV
                     DTVILAVEGLDRHLAPTRKID"
BASE COUNT          266 a          618 c          585 g          250 t
ORIGIN      
        1 ggagcgggaa gccatgtcag gagaggaaga ggtacggctt ccacgtgtcg tcgagcgtgc
       61 ccacgaaatc acggatgaac atgacgtggc tcggccggaa cggacgccgc cgaaggggca
      121 tgcccgcctc gtccggcgtc cggctgccct tccggttgtt gcagggcacg cacgccgtga
      181 ccaggttctc ccacgagtcg cgtccgcccc gtgaacgggg catgacgtgg tcgatcgtga
      241 gcttgtcgcg gttcccgcag tactggcagc ggtttcggtc ccgccgcatg acgttcttgc
      301 gcgtgagcgt gatccgcttg aacggaatgg tcacgtacgc gcggagccgg acgatgctcg
      361 gccagggcac gcgccgcgaa ggggaccgga gccaccggtc gggctccgcc tcgacgagct
      421 cggccttctg gagcaggacc aggatggtcg cccgttccac gctgcatacg gtgagggcgc
      481 ggtaatcctg gttcaggacg aggacgtgcc cgttcatgac acgccctccg gggttgcccg
      541 ggccggttcg ggccggtatc ggccgtgcgg ctcacgggtc gaagtgtatg gaccgaagac
      601 gcaccctccg cagaatgcaa taacggtttc ggttcgcggc tcaagcggcc ggtcggtaat
      661 tatcgcacag cgaacccgtt tccgcaagca cctcaggcgt ccctgataac ggaagcctcc
      721 gcccggcgcg ccagcacctc gatcgcgtcg cccacgcgca cctcgcccgc cgaccgcgga
      781 acgaggttct ccccgaacag gaccttgcgg ccgctcctgc ggaacgtgcc cagggcgcgc
      841 agcacccctc cgtctggctt tccggtcccg ggatcctggt cgatcacccg gcagcgggcg
      901 cacggcttgg ccacgtcgaa ggtcacgctc ccgatgcgga tgctcgccca cccgtcctcc
      961 gcgaaggcct cggcgccgga catcaccagg ttcggccgga agcggcggtc ctccaggggg
     1021 cgccctacca cccgcgcgag ggcccggacc gaggcggcgt tcactagcag cgccgggtag
     1081 gcgtcggcga atcccaccac gtcgcccgga cgcgcgaact ccggctccac ggggcggagg
     1141 tcggcctcgg gcacgaacac cagtgcgagg gcgcggccga ggcgctccga aagccacgcc
     1201 gagacgtcat cccgggcctc gagcgcgcgc acgtgctggc cccagatttc cacgtccatc
     1261 ggccggccgg ccgcccacga tttcggcagg atgcaaccgt ccccggcgcc atgccgaagc
     1321 tcccaggcat caaaggcgtc ggccacgcgc agcccggcca gggccggctc ctggcgcctc
     1381 gtcacgaagc gtccgtccgg ctccacgacc atccaccgcc gatcgtggcg cagaccggcc
     1441 gcctggacct gggcccggtc gagccggacc tcgccgccgg acttcacggg gtagatgcgg
     1501 atcgattcca ggcgcacggg ttcagtcgat cttgcgggtc ggggccaggt gccggtccag
     1561 tccttcgacg gccaggatca cggtatcgac ggcccgcgca aagaaatccg cgtcgatccg
     1621 ctcggtgtcg tccgacggcc ggtggtaatc ctcgtggtcc tcgacgccga agtagacgaa
     1681 cgggatcccc gcccggtgga acgccccgtg atcgctctg
//
LOCUS       DBNHQZ010000372         3397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_789940, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000372 DBNHQZ010000000
VERSION     DBNHQZ010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_789940"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="AC16YU_10065"
     CDS             complement(<1..749)
                     /locus_tag="AC16YU_10065"
                     /inference="COORDINATES: protein motif:HMM:NF020028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR6065614.1"
                     /translation="MTGAEDVLWDLTHLHPDADAVREALRAVGTDSEAFASEFRGRVA
                     SLDAPDLARALEALGSIQDRAGRAYAYAYLDWSTDTADSARGALLQHVREAYTAIARR
                     ILFFELEWTKVDDDRAGDLLREPSIAPYRHFLDVQRLQRDHLLSEPEESLLVEKSLTG
                     AQAWTRFFDETVGAVRFHVDGEELTQQQVLARLHEPDREVRKAAALAFTDGLGRELRT
                     LTFVFNTLLADKHSDDRLRRFEHWLAARNLS"
     gene            854..1474
                     /locus_tag="AC16YU_10070"
     CDS             854..1474
                     /locus_tag="AC16YU_10070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6065615.1"
                     /translation="MMRLIPSKSFAGGRQPLRGGPAGAIAAAALLVVLVAACGGAEPR
                     GPRPDPREIVVGSGAPSVTATSYPLPGLELHAMDGRRLATNDLRGRVIVVNFWATWCG
                     PCLREIPDLVRLSGEYAATPLDVIGVALNDPDSTAVSAFVRDHGMTYPVVVDHDGRIA
                     DALGGVFVMPTTFIVDPGGTVVRRIAGIAHPDSLRADVERLLAAGG"
     gene            complement(1484..2770)
                     /locus_tag="AC16YU_10075"
     CDS             complement(1484..2770)
                     /locus_tag="AC16YU_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6065616.1"
                     /translation="MPHDAFERLESVAGITEFRHRGNGLRVLHLPDTSTPTATVMVTY
                     LVGSRHEPEGLTGATHYLEHLMFKGTDRFNRDSGSSVFQTLQRFGAQVNATTWVDRTN
                     YYAMLPSARVGTAMAVEADRMRGARIRPADVEDERTVILNELDRGENEPLRRLVHAVF
                     RTAFEVHPYRHPTIGWREDVASVTAEALRGFYDTYYWPDNAVVSVIGDVTEEAALGLV
                     HEHFGAIPPAPSPVPPVTAVEPPQAGERRTTVSMTGEPPAVLMAWKAPRGTDPDADAL
                     GLLAMVLSSGRMSRLYRALVDTGLAISQSVTVTRFRDPGLFYAYVMLAPGTGHDRIED
                     AVRHELSELLANGVTAGELERARAQLRAHEAFSRDGPYAMAGQLNESIAAGDWRLFAT
                     LRERTGRLGRDEVGDAAARFLQAERLTVGRFVPVQA"
     gene            complement(2772..>3397)
                     /locus_tag="AC16YU_10080"
     CDS             complement(2772..>3397)
                     /locus_tag="AC16YU_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR6065617.1"
                     /translation="AMQEAFRTGADIHTATAARVFSCPLDEVTRDQRRKAKEVNYGIP
                     YGVSSFGLAQRLRCSTGEAQALIDGYHASFPRVSRFLADQVELARERGYAETTLGRRR
                     FVPDIGARNRNVRSAAERVAVNMPIQGTQADMIKIAMVRIHERIRKDGLQARMLLQVH
                     DELVFEAPPPEMGALRRLVREEMESALPIGVPVEVGMDEGENWLDAH"
BASE COUNT          480 a         1208 c         1169 g          540 t
ORIGIN      
        1 ttggacaggt tgcgcgcggc gagccagtgt tcgaagcggc gcagccggtc gtcggagtgc
       61 ttgtccgcga gcagcgtgtt gaagacgaag gtcagcgtcc ggagctcacg cccgagcccg
      121 tccgtgaatg ccagggcggc ggccttgcgc acttcgcggt ccggctcgtg cagccgcgcg
      181 agaacctgct gctgcgtgag ctcctccccg tcgacgtgga agcgcacggc accgacggtc
      241 tcgtcgaaga accgcgtcca cgcctgggcg cccgtcagcg acttctcgac caggagggac
      301 tcctccggct ccgagagcag gtggtcccgc tgaagccgct gcacgtcgag aaagtgccga
      361 tacggggcta tcgagggctc ccggagcaga tcaccggcac ggtcatcgtc gactttcgtc
      421 cactcgagct cgaagaagag gatgcgcctc gcgatggccg tgtacgcctc gcgcacgtgc
      481 tgaagcagtg ccccgcgcgc ggagtcggcc gtgtcggtcg accagtccag gtaggcgtac
      541 gcgtacgccc ggcccgcccg gtcctggatc gatccgagcg cctcgagcgc ccgggccagg
      601 tccggcgcgt cgagggatgc gacgcggccg cggaattcgg atgcgaaggc ctccgaatcg
      661 gtcccgaccg cccggagggc ttccctgacg gcatccgcgt ccggatggag gtgggtcagg
      721 tcccagagga cgtcctctgc gccggtcacg atcggtgctg gtttacgggg catgtgcgct
      781 gctctggatt ggacggcacg gccgggttct gaacctgccc gtgaaagggc ggttcaacgc
      841 gccaatcccg ggcatgatgc gcctgattcc ttcgaaatcg ttcgccggcg gccggcagcc
      901 attgcggggc ggacccgcgg gcgcgatcgc cgccgccgcg ctcctggtcg tgctcgtcgc
      961 tgcgtgcggc ggggcggagc ctcgcggtcc ccgccccgat ccgcgggaga tcgtcgtggg
     1021 ctccggtgcg ccgtctgtca cggccacgtc gtaccccctt cccggtctcg agctgcacgc
     1081 aatggacggt cggcgcctcg ccacaaacga tctgcggggc cgggtgatcg tcgtcaactt
     1141 ctgggcgaca tggtgcgggc cgtgcctccg ggagattccc gacctcgtcc gcctgtccgg
     1201 agagtacgcg gccactccgc tcgatgtgat cggcgtcgcc ctgaacgacc cggattccac
     1261 ggccgtatcc gcgttcgtcc gcgaccatgg catgacgtat cccgtcgtgg tggatcacga
     1321 cggtcggatc gcggacgcgc tgggcggtgt gttcgtcatg cccacgacct tcatagtcga
     1381 tcccggcggc acggtggtgc gccggatcgc gggcatcgcg catccggatt cgctgcgcgc
     1441 ggacgtggag cgcctgctcg cggccggggg atgagcgcag gcctcaggcc tggaccggca
     1501 cgaaacggcc caccgtgaga cgttcggcct gcaggaagcg tgcagccgca tcgccgacct
     1561 cgtcccgccc gagtcgcccg gtccgctccc gcagcgtggc aaagaggcgc cagtcgccgg
     1621 cggcaatcga ctcgttcagc tggcccgcca tcgcatacgg cccgtcccgg ctgaacgcct
     1681 cgtgcgcccg cagctgcgcc cgtgcacgct cgagctcccc cgccgtgacg ccgttcgcca
     1741 ggagctccga cagttcgtgc cggaccgcat cctcgatccg gtcgtgaccg gttccggggg
     1801 cgagcatcac gtacgcatag aagagcccgg ggtcgcgaaa gcgcgtcacg gtcacagact
     1861 ggctgatcgc caggccggta tcgaccagcg cccggtacag gcggctcatg cgtccggacg
     1921 acagtaccat cgccagaaga cccagcgcgt ccgcgtccgg atccgtgccg cggggcgcct
     1981 tccaggccat gagcaccgcg ggcggctcgc ccgtcatgct gaccgtggtg cgccgctccc
     2041 ccgcctgggg cggctccacc gccgtgaccg gcggaaccgg ggacggagcc ggtgggatcg
     2101 cgccgaaatg ttcgtggacg agcccgagcg cggcttcctc cgtcacgtca ccgatcaccg
     2161 acacgacggc gttatccggc cagtagtacg tgtcgtagaa gccgcgcaac gcctccgcgg
     2221 tgaccgaggc cacgtcctcg cgccatccga tcgtcggatg ccggtaggga tgcacctcga
     2281 acgccgtccg gaacacggcg tggaccaggc gtcggagcgg ctcgttttcc cctcggtcca
     2341 gctcgttcag gatgacggtc cgctcgtcct cgacgtccgc cggccggatg cgcgcgccac
     2401 gcatgcggtc cgcctcgacc gccatggccg tcccgacgcg ggccgaaggc agcatggcgt
     2461 agtagttcgt ccggtccacc caggtcgtag cgttcacctg cgcgccgaaa cgctggagcg
     2521 tctggaacac ggacgatccc gaatcccggt tgaaccgatc cgtgcccttg aacatcaggt
     2581 gctccaggta gtgggtcgcg ccggtgagcc cttcgggttc gtgcctcgat ccgacgaggt
     2641 acgtgaccat cacggtagcc gtcggcgtcg acgtgtccgg gaggtgcagc acccggaggc
     2701 cgtttccgcg gtggcggaac tccgtgatgc cggcgaccga ttccaggcgt tcgaatgcgt
     2761 cgtgcggcat ctcagtgcgc gtcgagccag ttctcgccct cgtccatgcc cacctcgacc
     2821 ggcacgccga tcggcagcgc ggactccatt tcctcgcgga ccagcctccg gagcgccccc
     2881 atctcgggcg ggggcgcctc gaagacgagc tcgtcgtgaa cctgtagcag catccgggcc
     2941 tgcaggccgt ccttccggat gcgctcgtgg atgcgcacca tcgcgatctt gatcatgtcg
     3001 gcctgcgtgc cctggatcgg catgttgacg gccacgcgct cggccgcgga gcggacgttc
     3061 cggttccggg ccccgatgtc ggggacgaag cgccgccgcc cgagcgtcgt ctcggcgtat
     3121 ccgcgctccc gggccagctc cacttggtcc gcgaggaatc gagacacgcg cggaaacgag
     3181 gcgtggtacc cgtcgatcag cgcctgcgcc tcgccggtcg agcaccgcag ccgctgggcc
     3241 agcccgaagg acgacacgcc gtatggaatc ccgtagttca cctccttggc cttgcgccgc
     3301 tggtcgcgcg tgacctcgtc cagcggacac gagaagaccc tcgccgccgt ggccgtgtgg
     3361 atgtccgcgc cggtccggaa ggcctcctgc atggcct
//
LOCUS       DBNHQZ010000373        13466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1063943, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000373 DBNHQZ010000000
VERSION     DBNHQZ010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13466
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1063943"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="AC16YU_10085"
     CDS             <1..1213
                     /locus_tag="AC16YU_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6065618.1"
                     /translation="AALVAIGLVAIYSTTHGPAAEFLLESVRRNYDRQMVWGAICLVG
                     ILVALLLPVRLVQKSAPLVYLGAIGLLVAALLFGREINGARSWLTLGPVQLQVSEFAK
                     FGAVLGVAYLVGSRRPGTPGLRFALAIIGLLLVPALLIILQNDTGTALVFIGLIPVML
                     FWAGVPVDLLMLLLAPAVTGYLAIVYEPAAIAFVVVFTLFIYIRTRDRRLTVLAALFT
                     GGTLGAVAVALTRILQPHQVARVLSFTRPGAEEFRHDVGFHLVQSLAAIGSGGLSGKG
                     FMQGTQTQGRYVPEQSTDFVFSVIGEEFGFVGSALVLLLFLLLLVRLVMLGSQMKHPF
                     GMMVAAGTAGIYLVHVFVNVGMVTAVLPVIGIPLPFISYGGSALMAHTALLAVTLSLH
                     MRRDDFSIYVY"
     gene            1289..2362
                     /locus_tag="AC16YU_10090"
     CDS             1289..2362
                     /locus_tag="AC16YU_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR6065619.1"
                     /translation="MRPSAALFATLLLVALAVLLAREAASPETDPLVATFSMVAFDPE
                     TGDLGVVVQSKFPNLRPVVPWLRAGVGAVATQSFAELDYGIRGLDLMENGATAEEALR
                     IVLRGDPDRQERQVGIVDASGNAATWTGTGAFDWKGARIGSSDGAVTAVGPEQGNVGT
                     VATGWGYTAQGNILVSAETVEAMVRAYEQTGGELAARLVAALVAGGKAGGDQRGEQSA
                     ALVVVRKGAGYDGMDNYIDISVYDHPTPIAELERLYRLNNLYFTQSRPENMIPVDPAI
                     ARELQEIWMERGFYDGPPDGVVDEEFRQILVDFMGWENYDLRIAPVRETDLAAGETLR
                     IDHEVLDDIRAVFREGRWLPRRR"
     gene            2421..2606
                     /locus_tag="AC16YU_10095"
     CDS             2421..2606
                     /locus_tag="AC16YU_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6065620.1"
                     /translation="MSRERTRECPACALEGPADADTCPYCGYEYPPRPTRALVWAAIV
                     MAALLAWPVIRILRSLF"
     gene            complement(2768..4306)
                     /locus_tag="AC16YU_10100"
     CDS             complement(2768..4306)
                     /locus_tag="AC16YU_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6065621.1"
                     /translation="MKTRLPIILMAVVTALPWLGQTALAQDSADGSWYLRPRIGVSYY
                     MGDNGTGMSFSSDDQFPLHAGLEVGNNFNSTFSVGLAGAYSAFDDINDFDSNDDTPGD
                     GTRIAVQLIARKRLGTGSVAPFIQFGGGVSFGKTKLYENFVADTNVDTEESKVAFGPL
                     LGIGLDFRLSDAVSFFVETGASYLFGDVTSDGREDDEIPEGEGFGPGDLLGWHALGFK
                     FNLNTFTPVMVTDLICPEGVVDTGDPVTLTGSVNEKASRPVVGSWDFGDGTTATGMTA
                     THGFRPEGTYTVTFTASNGNGRATDSRSCRVTVEDPCETAEIISISPSSMTPDTRTAV
                     RFSGNVRGSAPITYAWDFGDGATSAEANPTHTYAEPGNYTVTLTVTNCGGTVSRTMEI
                     SVAMYEAAICREIEEMNPVFFDRNSSTLSEEARGKLQENLEILGECPNLNARVEGWAA
                     PGERNTQGLSADRAKAVEQFYVDNGIALSRLMTVGMGRAEGMTSKKEGLAGYRRVDTI
                     PVRN"
     gene            complement(4381..5655)
                     /locus_tag="AC16YU_10105"
     CDS             complement(4381..5655)
                     /locus_tag="AC16YU_10105"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR6065622.1"
                     /translation="MEIAKLNLRDKEYDLPVIVGSENEVGLDIRKLRGQSGAITFDSG
                     YGNTGSCDSAITFINGEEGILRYRGYPIEQVSEHSSFLEASYLVMYGELPTKTQLDNF
                     QDRLTHHSLLHEDMKKFFEGYPPSAPPMSVLSTIVASLNGFYEDDDDPDLRELNIIRL
                     IAKIKTIAAFSYKKSIGQPYIYPRNDLDYSSDFLHMMFAVPSETYEVSPLLAKTLDLL
                     LILHIDHEQNCSTSTVRMVGSSGASLFASMSAGISALSGPLHGGANVQVIAMLENIVK
                     DGGDVSKYINMAKDKDSGFKLMGFGHRVYKNFDPRARVIKSYADAVLNDMGVKDPLLD
                     MAKQLEQAALADDYFAERKLYPNVDFYSGILYRAMGIPTNMFTVMFALGRLPGWIAQW
                     LEMKNDPTTRIYRPRQIYTGATERNYVPMERR"
     gene            5876..6586
                     /locus_tag="AC16YU_10110"
     CDS             5876..6586
                     /locus_tag="AC16YU_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6065623.1"
                     /translation="MQETTSGRRAAETAFGHPRLERAWEAIAEAVLERPSTADLFNPY
                     RDRVDGLDRPDAVAVRRANLRSYLAAYRRPPALFLLAEAPGPWGCRFSGVPITAEAQL
                     LDPGFPLSGGRSSLGEEPRSEYSAGIFWRILEPAFPHFLVWNAVPLHPHRAGEPLSIR
                     TPRAGELLEFQPLVRELVSMLAPRRVLAIGRKAELVLGRSEIPCTYVRHPSQGGAVLF
                     AEGVRAALGEAGITPGRV"
     gene            6641..7954
                     /locus_tag="AC16YU_10115"
                     /pseudo
     CDS             6641..7954
                     /locus_tag="AC16YU_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007101493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            8177..8740
                     /locus_tag="AC16YU_10120"
     CDS             8177..8740
                     /locus_tag="AC16YU_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065624.1"
                     /translation="MRRLSAFALVLMLAAAGCAGDSMLVREDPADVAAKSPRLVPIEG
                     LFTFLPDLAGGLVDCGGGFVFPHLYRAKGDISHLGHTSVLITLDECWLEAEPVPHLVG
                     VGTGEYTAANGDQLYGEYVMPTYLDPEGGPMATYDLDPIEYVGGTGRFDGVTGHAVGG
                     GTMSIVTGAGSYWIQGMLASVGSLKQE"
     gene            complement(8764..10026)
                     /locus_tag="AC16YU_10125"
     CDS             complement(8764..10026)
                     /locus_tag="AC16YU_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065625.1"
                     /translation="MTYNHVCRSVVLFALAIVPLAIAACDSSSDLGNGEAIRITGVVT
                     DDAAAKAQGEVENADVRARKVDPDGDTEPAGGSTTTDSQGRYDLAASSRTELMLVTAV
                     RDGFETRTMVDTRSEAGGEVGAVSMTSETRAEADVFLEARQAGRAHVGLGEVAVWVTS
                     VVAARMDAGAATAAEVSAAVGTAEEARRDYLAAEHAATAQVLASMDADEEASFRAYQT
                     VVSGSTSGSVHANALAGLRAAISTSGSDLHADARSREAARAALVALFQDLDLEIRTAM
                     RQEAELSTAIATRAAVDAGFQAMGSANAQAVVQAGSVLSEALASADDDVEFAAAWEAY
                     ASTVRGHLAAELAITTGLMDLAVGALSTLEATLEASLDAATTVDAVVEAKAAYFNGAE
                     LSLRTTLDADTSPDAEIASGLLVLVEVH"
     gene            10222..10803
                     /locus_tag="AC16YU_10130"
     CDS             10222..10803
                     /locus_tag="AC16YU_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6065626.1"
                     /translation="MKLLLRIASAIDRFSDRTGRIIVWLTLLMVLAGGYNAVVRYLDK
                     FTGLGLSSNTYIELQWYLFSLVFLLGAAYTLRHDAHVRVDVLLVRLSPKGRAWVDVMG
                     TVLFLVPFCILMLWVAWPAVSSSWAVREMSPDPGGLPRYPIKAVIPVAFILLLVQGVS
                     LFIRSLAVITGHDGPGTAAAPGTAAAPHAGGHV"
     gene            10818..12122
                     /locus_tag="AC16YU_10135"
     CDS             10818..12122
                     /locus_tag="AC16YU_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6065627.1"
                     /translation="MGPLMFVGALLLIFSGFPVAFALGGTAMIFALVGVGTGILDWHL
                     LMALPDRTFGVMSNYVLLAVPFFIFMGTMLEKSRLAEDLLTTIGALFGPVRGGLALAV
                     VFVGALLAAATGVVGASVVAMGLISLPVMMQYGYGKELSAGVITASGTLGQIIPPSVV
                     LVVLADQLGVSVGDLFIGALVPGLILALLYAGYAGGVAIFRPKLAPALPAEARGGHAK
                     GLGRRVLTVMLPPLVLILLVLGSIFAGIATPTEAGALGSLGAIGLAAANRRLTLAALR
                     ETMTETTKLTSMVMFLLIGSTAFALVFRGLNGDLWIHGLLTGLPGGVIGLLIVANLTI
                     FVLGFFIDFFEIAFIVIPLLAPAAYALGIDLVWFGVMIGMNLQMSFLTPPFGFALFYL
                     RGVAPPGITTAQIYRGAVPFIAIQAVGLVLIILFPGLVTWPF"
     gene            12346..12819
                     /locus_tag="AC16YU_10140"
     CDS             12346..12819
                     /locus_tag="AC16YU_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6065628.1"
                     /translation="MLEALDRTDRRILNILQSDGGITNVELSRQVGLAPATTLDRVRK
                     LRQNGFILGYVALVDSAMVEQSTVAFVAVSLTEHGAEKVEAFRAHIESLPEVLECYHV
                     SGESDFLLKIVARDIRTYEDFLLHRLTSIPCLGRIHTSFVLSTVKHDTRIPIDES"
     gene            12841..>13466
                     /locus_tag="AC16YU_10145"
     CDS             12841..>13466
                     /locus_tag="AC16YU_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6065629.1"
                     /translation="MDDSDRHYDPTTRLIHGRMRSEAWEYGHHLVPPISTSTTFRLDS
                     TSRGAMGFEEFGHPEGPDHHIYIYDRLHEPNKDLLEERLAAAEGGEVAVTFASGMAAI
                     SAALGVLTKAGDHVLTHRAMYGCTHSLLHRWYPRMNVAVSDADLRDVEGAVREIRPET
                     RVVYLETPANPTMDIIDLASLAAEIHLLNTKRDESERIWLVVDNTFSTP"
BASE COUNT         1983 a         4509 c         4613 g         2361 t
ORIGIN      
        1 ggcggccctc gtggcgatcg gcctcgtggc gatctacagc acgacccacg ggccggcggc
       61 ggaattcctg ctcgagagcg tgcggcgcaa ctacgaccgc cagatggtgt ggggagcgat
      121 ctgcctcgtc ggcatcctgg tcgcgctgct cctgcccgtc cgcctggtcc agaaatcggc
      181 cccgctcgtc tacctggggg ctatcgggct gctcgtcgcg gccctgctgt tcggccggga
      241 gatcaacgga gcgcgatcct ggctgacgct ggggcccgtg cagctgcagg tgtccgagtt
      301 cgcgaagttt ggcgccgtgc tcggcgtggc ctacctggtc ggctcacgcc gccccggtac
      361 gcccggcctc cggttcgccc tggcgatcat cggactgctg ctcgtgccgg cgctcctcat
      421 catcctgcag aacgacacgg gcacggcgct ggtgttcatc gggctgattc cggtgatgct
      481 gttctgggcg ggcgtgccgg tggacctcct gatgctgctg ctggccccgg cggtgaccgg
      541 ttacctggcc atcgtgtacg aacccgccgc gattgccttc gtggtcgtat tcacgctgtt
      601 catctacatc cggacccgtg accgcaggct cacggtcctt gcagccctgt tcaccggcgg
      661 cacgctcggc gcggtcgccg tcgcgctcac ccgtatcctg cagccgcacc aggtcgcgcg
      721 ggtcctgtcg ttcacgcggc ccggtgcgga ggaattccgc catgacgtgg gtttccacct
      781 tgtgcagtcg cttgccgcga tcgggtcggg aggactgtcg ggcaagggat tcatgcaggg
      841 aacccagacg cagggccggt acgtccccga gcagtcgacc gacttcgtgt tctcggtgat
      901 cggggaggag ttcggattcg tgggctcggc cctcgtgctg cttctcttcc tgctcctgct
      961 cgtccgcctc gtcatgctgg gaagccagat gaagcacccg ttcggcatga tggtcgcggc
     1021 gggcaccgcc ggcatctacc tcgtccacgt cttcgtgaac gtggggatgg tcaccgccgt
     1081 cctgccggtg atcggcatcc cgcttccctt catctcgtac ggcggttcgg cgctcatggc
     1141 ccatacggcg cttctcgccg tcacgctttc cctgcacatg cggcgggacg acttctccat
     1201 ctacgtgtac tgacggggcg ggctcgcggc gttgcggttc tgtgccgcga ggcggacttt
     1261 tgggacacgc atccgaccga ggcatgccat gcgcccgtcc gccgcgctct tcgccaccct
     1321 gctgctcgtt gcccttgcgg tactcctcgc ccgggaggcc gcctcccccg agacggatcc
     1381 gctcgtcgcc accttctcga tggtggcctt cgacccggag accggtgacc tgggggtcgt
     1441 ggtccagtcc aagttcccga acctcagacc ggttgttccc tggctcaggg ccggcgtggg
     1501 cgccgtggcc acgcagagct tcgccgagct cgactacgga atccgcgggc tggacctcat
     1561 ggagaacggg gcgaccgccg aggaggccct gcgcatcgtg ctccggggcg acccggaccg
     1621 gcaggagcgc caggtgggca tcgtggacgc ctcgggcaat gcggccacgt ggaccgggac
     1681 cggcgcgttc gactggaagg gggcgcgcat cggctcgtcg gacggcgccg tgacggccgt
     1741 cggtccggag cagggcaacg tggggaccgt tgcgaccggc tggggataca cggcccaggg
     1801 caacatcctc gtctccgccg aaacggtcga ggccatggtc cgggcctacg agcagaccgg
     1861 cggggagctg gcggcgcggc tcgtcgccgc gctcgtcgcg ggcggaaagg cgggcggaga
     1921 ccagcgcggg gagcagagcg cggcgctcgt cgtggtccgc aagggcgccg gctacgacgg
     1981 catggacaac tacatcgaca tctcggtcta cgaccacccg acgcccatcg ccgagctcga
     2041 gcgcctgtac cgcctgaaca acctgtactt cacgcagtcg aggcccgaga acatgatccc
     2101 ggtcgatccg gcgatcgcga gggagctgca ggagatctgg atggagcggg ggttctacga
     2161 tggtccgccg gacggggtcg tggacgagga attccggcag atcctggtgg atttcatggg
     2221 ctgggagaac tacgacctgc gcatcgcccc ggtgcgtgag accgatctcg cggccggcga
     2281 aacgctccgc atcgaccacg aggtgctcga cgacatccgg gccgtcttcc gggaaggccg
     2341 atggctgccc cgccggcgat aaccgcgcct gagctcccgc ccgcacgggt gtatccttcg
     2401 ccccgaacga cctggcaccc atgagccgag aacgaacgcg ggaatgcccc gcctgcgccc
     2461 tggaaggacc ggcggacgcc gacacctgcc cgtactgcgg ctacgagtat cccccgcggc
     2521 cgacgcgcgc cctggtctgg gccgcgatcg tcatggccgc cctgctggcc tggcccgtga
     2581 tccggatcct gcgctccctg ttctgaaggc ggatcggccg atcgggcgtc cgcgaaggcg
     2641 gggcagacgg ccgggagact ccgggatccg gacctccgga tacgcgcggg tccgcgctgc
     2701 cctgaaatgc ggagggcgga cccgccgaag cggatccgcc ctcgcattct tcaggattcc
     2761 cttccgccta gttgcggacc gggatcgtgt ccacgcggcg gtagccggca aggccctcct
     2821 tcttggaggt catgccctcg gcccggccca tgcccaccgt catcaggcgg ctgagcgcga
     2881 tgccgttgtc cacgtagaac tgctcgacgg ccttcgcacg gtcggccgag aggccctgcg
     2941 tgttgcgctc gcccggggcc gcccagccct cgacgcgggc gttgaggttc gggcactcgc
     3001 cgaggatctc caggttctcc tggagcttcc cgcgggcctc ctcggacagc gtcgaggagt
     3061 tgcggtcgaa gaagaccggg ttcatctcct cgatctcgcg gcagatggcc gcctcgtaca
     3121 tggcgacgct gatctccatg gtccgggaga cggtgccgcc gcagttggtc accgtcaggg
     3181 tcaccgtgta gttgcccggc tcggcatacg tgtgcgtcgg gttcgcctcc gcggacgtgg
     3241 cgccgtcacc gaagtcccag gcgtacgtga tcggcgcgga gccgcggacg ttgcccgaga
     3301 accggaccgc ggtccgcgta tccggggtca tgctcgacgg gctgatcgag atgatctcgg
     3361 ccgtctcgca cggatcctcg accgtgaccc tgcagctccg gctgtcggtc gcgcggccgt
     3421 tgccgttcga cgccgtgaag gtcaccgtgt aggtgccctc cggacggaag ccgtgggtcg
     3481 cggtcatgcc cgtggccgtc gtgccgtcgc cgaagtccca ggagccgacg accggccgcg
     3541 aggccttctc gttcaccgag cccgtcaacg tcaccgggtc accggtgtcc accacgccct
     3601 cggggcagat caggtcggtc accatgaccg gcgtgaacgt attgaggttg aacttgaagc
     3661 ccagcgcgtg ccagcccagc aggtcgccgg ggccgaagcc ctcgccctcc gggatttcgt
     3721 cgtcttcccg cccgtccgac gtcacgtcgc cgaacaggta cgaggcgccc gtctccacga
     3781 agaacgacac cgcatcgctc agcctgaagt cgaggccgat gccgagcagg gggccgaagg
     3841 ccaccttgct ttcctcggtg tcgacgttcg tatcggcaac gaagttctcg tagagcttcg
     3901 tcttgccgaa gctgacgccg ccgccgaact ggataaacgg cgccacggag cccgtgccga
     3961 gccgcttccg ggcgatgagc tgcacggcga tgcgcgtgcc gtcgcccggg gtatcgtcgt
     4021 tgctgtcgaa gtcgttgatg tcgtcgaaag cgctgtacgc tccggccagg ccgaccgaga
     4081 acgtcgagtt gaagttgttc ccgacttcca gacccgcgtg aagcgggaac tggtcgtccg
     4141 agctgaagct catcccggtg ccgttgtcgc ccatgtagta ggagacacca atccgcggcc
     4201 gcaggtacca gctgccgtcg gcgctgtcct gcgcgagggc cgtctggccc agccagggca
     4261 gggcggtcac caccgccatc aggatgatcg ggagccgagt tttcatcgtg ttcctccgaa
     4321 gaacaggtag ttagcggtca agtggtcctg gtaatgcacg ttcttgcgcg cctttgcgcg
     4381 tcaacgccgt tccatgggca catagttcct ttcggtcgcg cccgtgtaga tctggcgcgg
     4441 gcgatagatc cgcgtcgtcg ggtcgttctt catctcgagc cactgggcga tccagcccgg
     4501 gagtcgcccg agagcgaaca tcaccgtgaa catgttggtt ggtattccca tggcacgata
     4561 aaggattccg ctgtagaaat ccacatttgg atacagtttg cgctcggcga agtagtcgtc
     4621 ggcgagtgcg gcctgctcca gctgcttcgc catgtcgagc agcgggtcct tcacccccat
     4681 gtcgttcagc acggcatcgg cgtacgactt gatgacgcgc gcgcgcgggt cgaagttctt
     4741 gtagacgcgg tgtccgaagc ccatgagctt gaaccccgaa tccttgtcct tggccatgtt
     4801 gatgtacttg gacacgtcgc cgccgtcctt cacgatgttc tccagcatgg cgatcacctg
     4861 cacgttcgca ccgccgtgca gtggtccgga cagggcgctg atgcccgccg acatcgacgc
     4921 gaagaggctc gcgccgctgg aacccaccat gcgcacggtc gatgtcgagc agttctgctc
     4981 gtggtcgatg tgcaggatca ggagcaggtc cagcgtcttc gcaagcagcg gcgagacctc
     5041 gtaggtctcc gatggcaccg caaacatcat gtgcaggaag tcggacgagt agtccaggtc
     5101 gttcctgggg tagatgtacg gctggccgat tgacttcttg tacgagaagg ccgcgatcgt
     5161 cttgatcttg gcgatgagcc ggatgatgtt cagctcgcgg aggtcgggat cgtcgtcatc
     5221 ctcgtagaag ccgttgagcg aggccacgat ggtcgagagg acgctcatcg ggggggcgct
     5281 cggcgggtac ccctcgaaga acttcttcat gtcctcgtgc agcaggctgt gatgcgtgag
     5341 ccggtcctgg aagttgtcca gctgcgtctt cgtcggcagt tcgccgtaca tgaccaggta
     5401 cgaggcctcc aggaaggagg agtgctcgga gacctgctcg atcgggtacc cgcggtaccg
     5461 caggatgcct tcctcgccgt tgatgaaggt gatggcgctg tcgcacgagc ccgtgttgcc
     5521 gtatcccgaa tcgaacgtga tggcgcccga ttgtccgcgg agcttgcgga tgtcgaggcc
     5581 gacctcattc tcgctgccca cgatgacggg gaggtcgtat tccttgtccc tgagattcag
     5641 ttttgcgatt tccatcttgt cacctgccgg tccagtaatc ggggagtggt taattgttcg
     5701 aagctaacgg acggggcatg gaggcgctgt cggggctttg cttgcatttc cgtctgcaac
     5761 gaaaattcgc tgactgtcag gttttcgctg acattcctgc ccgggagggg tcgaacggac
     5821 ggtccgccgc ttcccgggtc cgcggcctac cttgccggaa gtcgaacgga gagccatgca
     5881 ggagactacg agcggccggc gcgcggccga gacggcgttc gggcatcccc ggctcgagcg
     5941 cgcgtgggag gcgatcgcgg aggcggtgct cgagaggccg tcgaccgccg acctcttcaa
     6001 cccgtaccgc gaccgcgtcg acgggctgga tcggcccgac gccgtggccg tccgcagggc
     6061 caacctgcgg tcgtacctcg ccgcgtaccg gcggccaccg gccctcttcc tgctggccga
     6121 ggcgcccggg ccgtggggct gccgcttctc cggggtgccg atcacggcgg aagcccagct
     6181 cctggacccc ggcttcccgc tgtcgggcgg ccggtccagc ctcggagagg agccccgctc
     6241 cgagtacagc gccggaatct tctggcggat cctggagcct gccttccccc acttcctcgt
     6301 atggaacgcc gttcctctgc atccgcaccg ggcgggggag ccgctctcga tccggacgcc
     6361 gcgggcgggg gagctcctgg agttccagcc gctcgtgcgg gagctcgtat cgatgctggc
     6421 gccgcggcgc gtgctggcca tcgggcgaaa ggcggagctg gtcctcgggc ggtcggagat
     6481 cccgtgcacg tacgtccggc atccgtcgca gggcggcgcg gtgctcttcg ccgagggggt
     6541 ccgcgcggcg ctcggcgagg cgggaataac gccgggacgg gtctgagcgg gcgatcctgg
     6601 agggcgcgcg ggcgtatgcc tggtccatcc ggagcccgac atgtccgaca gcctgttcga
     6661 tgcgaccgcg gagaagtccc gcagccggaa cgccccgctc gccgaccgga tgcggccgcg
     6721 cacgctcgat gccttcgtgg gtcaggacca catcctgggc ccgggccgcc tgctgcggcg
     6781 gtccatcgag gccgaccgga tctcgtcgat catcttcttc ggccccccgg gcaccggaaa
     6841 gaccacgctc gcccgcatca tcgcgaacgg cacgcgggcc cacttcacgt cgatgaacgc
     6901 cgtgctggca ggcgtgaagg acatccggga agcgatcgcg gcggcgcagg accgcctccg
     6961 ctaccaccag cagcgcacca tcctcttcgt ggacgaggtg caccgattca acaaggcgca
     7021 gcaggacgcg ctgttgccgc acgtcgagaa tggaacggtg accttcatcg gggccacgac
     7081 ggagaacccg tatttcgaag tgatcaaggc gctcgtgagc cgatcgcgcg tgttcgagct
     7141 gcgggcgctg ggcgacgtcg acctggaacg cgtggcccgg caggccctct cggacgcgga
     7201 gcacgggtac ggccgactcg cggtggacgt ggacgaggac gcgctcgccc acctcgtctc
     7261 taccgcgaac ggcgatgccc gctcgctgct gaacgcgctc gagctggccg tcgagacgac
     7321 cgacgccgcc gaggacggca ggatccacgt ggacatggcc gcggcggagg attcgatcca
     7381 gaagcgggcg gtcctttacg acaaggaggg tgacgcccac ttcgactcga tctcggcctt
     7441 catcaagagc ctgcgcggct cggacccgga cgccgcgctc tactggctcg cccggatggt
     7501 gtatgcgggc gaggacccgc gcttcatctt ccggcgcatg ctgatcttcg cctccgagga
     7561 cgttgggctc gcggacccgc gcgcgatcca ggtcgcgacg gcgtgcatgc aggccttcga
     7621 gttcgtgggc atgcccgagg ggcgcttcca ccttgcggaa tgctgcctgt acctggcgac
     7681 ggcccccaag agcaacacga cgctctcgtt cttcgacgcc ctgagctacg tggagaagga
     7741 aagggcgtcc gacgtcccga accacctcaa ggacgccaac cgcgacaggg aggggctggg
     7801 gcacggggag ggataccagt atcctcacgc gtaccacaac cactacgtgc cgcagcagta
     7861 cctgccagac gcaatgcagg gcacgttctt ctatcagccc tccgacgtcg gattcgagcg
     7921 gacggtcgcg gagcgcatgg cgtactggcg cgagcgggac gagaaggaga gcctggagcg
     7981 gagggtgaag cggtacgcgg aggacgaggg cagtggcgag gaccatgtgg ggggcgagta
     8041 gacaccctgc gggacccgag gggcttcgcc ctacccggcc gggagatcgg acctacatga
     8101 atccgcgacg ttgtcctgca tcgggatagc gacgtggggg ctatcagtga ttcccccaat
     8161 agtcatgagg ttcaagatgc gaagactgtc tgcatttgcc ctggtcctga tgctcgcggc
     8221 ggccggctgc gcaggcgact cgatgctggt ccgggaggat ccggccgacg tcgccgcgaa
     8281 gtctcccagg ctcgtgccga tcgaaggact cttcacgttc cttcctgacc tggccggcgg
     8341 tctcgtagac tgcggcggcg ggttcgtatt cccgcatctc tatcgggcca agggtgacat
     8401 ttcccacctg ggccacacca gcgtcctcat cacactcgac gagtgctggc tggaggccga
     8461 gcccgtaccc catctcgtcg gtgtgggcac gggcgaatac accgcggcca acggcgatca
     8521 gctgtatggc gaatacgtga tgcccaccta tctcgatccg gaaggaggac cgatggcgac
     8581 ctacgatctc gaccccatcg agtacgtggg cggaacgggg cggttcgacg gcgtcacggg
     8641 acatgccgtg ggtggcggca cgatgtccat cgtgaccggg gccggcagtt actggatcca
     8701 gggcatgctc gcgtcggtcg gttcgctgaa gcaggagtag ggtcggaaga acctccgtcg
     8761 aggtcagtgc acctcgacga gaacgagaag tccggatgcg atctccgcgt ccggactggt
     8821 gtcggcgtcg agggtggtcc gaagggaaag ttcggccccg ttgaagtacg cggccttcgc
     8881 ctcgaccacc gcatcgaccg tcgtcgcggc gtcgagggag gcctcgagcg tcgcctcgag
     8941 cgtcgagagg gcgccgacgg ccaggtccat caggcccgtc gtgatggcga gttcggccgc
     9001 gaggtgaccc cggaccgtgg acgcgtaggc ctcccatgcc gcggcgaatt ccacgtcgtc
     9061 gtcggccgag gcgagcgcct cgctcagcac ggatccggcc tgcacgaccg cctgggcgtt
     9121 cgcagagccc atcgcctgga atccggcatc gacggccgcc ctcgtggcga tcgcggtgga
     9181 cagttcggcc tcctggcgca tggcggtccg gatctccagg tccagatcct ggaagagcgc
     9241 cacgagcgcg gcgcgcgccg cttcacggga gcgcgcgtcc gcatgcagat cgctgccgga
     9301 ggtggatatt gccgcgcgca ggccggcgag ggcgttcgcg tggacagagc cgctcgtcga
     9361 gccgctcacg acggtctggt aggcccggaa cgacgcctcc tcgtcggcat ccatggaggc
     9421 gagcacctgg gccgtggccg cgtgctcggc cgccaggtaa tcccggcggg cctcctcggc
     9481 ggttccgacc gctgccgaca cctccgcggc cgtcgccgcg ccggcgtcca tgcgggcagc
     9541 gaccacggag gtgacccaca cggccacctc cccgaggccg acgtgtgcgc gaccggcctg
     9601 gcgggcctcg agaaagacgt ccgcctcggc tcgggtctcc gaggtcatgc tcacggcgcc
     9661 gacctccccg cccgcctcgg agcgggtatc gaccatggtc cgggtttcga agccgtcacg
     9721 gacggccgtg acgagcatca gttcggtccg cgacgacgcg gcgaggtcgt atcgtccctg
     9781 ggaatcggtc gtggtcgacc caccggcggg ctctgtgtcg ccgtcagggt ccaccttccg
     9841 ggcgcggacg tcggcgttct cgacctctcc ctgcgccttg gccgcggcgt cgtcggtgac
     9901 gacgccggtg atccggatgg cctcgccgtt gccgagatcc gagctggaat cgcacgccgc
     9961 gatggcgagc ggcacgatgg ccagggcgaa caggacgact gatcggcaaa catggttgta
    10021 cgtcatctcg attgatgggc tggttgctgg gtgcggtcgg acgggcgggg cagcacccgg
    10081 tcctgtcagg tattaccgcg gccatccgat attcccctat cggccttgtc ccgacgaagt
    10141 tgaagcgcgg cccggcgggg gccgtggcgc gcccggtccc atgcgcctat tttcgccgcg
    10201 gacgcgcccc gacccggaac cgtgaagctg ctcctccgca tcgcgtcggc catcgaccgg
    10261 ttttcggatc ggaccggccg catcatcgtc tggctgaccc tgctcatggt cctggccggc
    10321 gggtacaatg cggtggtccg gtacctggac aagttcacag ggctcggact cagctcgaac
    10381 acgtacatcg agctgcagtg gtacctgttc tcgctcgtct tcctgctggg cgcggcgtac
    10441 acgctcaggc atgacgccca cgtgcgggtc gacgtcctcc tcgtccgcct ctccccgaag
    10501 ggccgcgcct gggtcgacgt gatgggcacg gtgctcttcc tcgtgccctt ctgcatcctg
    10561 atgctgtggg tggcgtggcc cgcggtgtcc agctcgtggg cggtccgcga gatgtcgccg
    10621 gaccccgggg gccttccgcg gtacccgatc aaggccgtca tacccgtcgc gttcatcctg
    10681 cttctcgtgc agggcgtgtc gcttttcatc cggagcctcg ccgtgatcac ggggcacgac
    10741 ggcccgggga ccgctgccgc ccccggaacc gctgccgccc cccacgcagg aggccacgta
    10801 tgacgcccga ggagttcatg ggccctctga tgttcgtcgg ggccctgctg ctcatcttct
    10861 cgggatttcc cgtggcgttc gcgctcgggg ggaccgcgat gatcttcgcc ctggtcggcg
    10921 tcgggacggg catcctggac tggcacctgc tgatggcgtt gcccgaccgg acgttcggcg
    10981 tgatgtcgaa ctacgtgctc ctcgccgtgc ccttcttcat cttcatggga acgatgctgg
    11041 agaagtcgcg cctggccgag gacctgctca cgacgatcgg cgccctgttc ggcccggtcc
    11101 ggggggggct ggcgctggcc gtcgtgttcg tcggggcgct cctggccgcc gcgaccggcg
    11161 tggtcggcgc gtcggtggtc gccatggggc tcatctcgct gccagtgatg atgcagtacg
    11221 gctacgggaa ggagctctcg gcgggcgtga tcacggcctc cgggacgctc gggcagatca
    11281 tcccgccgtc ggtcgtcctc gtggtcctgg ccgaccagct cggcgtctcg gtcggcgacc
    11341 tgttcatcgg cgccctggtg cccgggctga tcctggcgct cctgtacgcc gggtacgcgg
    11401 ggggcgtcgc catcttccgc ccgaagctgg ccccggcgct cccggccgag gcgcggggcg
    11461 gtcacgcgaa agggctcggc cggcgcgtcc tgaccgtcat gctcccgccg ctggtgctga
    11521 tccttctcgt gctggggtcc atcttcgccg ggatcgcgac gcccacggag gcgggggcgc
    11581 tcggctccct gggggcgatc gggctggccg cggcgaaccg caggctgacg ctggcggcgc
    11641 tccgggaaac gatgaccgag accacgaagc tgacgtccat ggtgatgttc ctgctgatcg
    11701 ggtccacggc cttcgcgctc gtgttccggg gactcaacgg cgacctgtgg atccacggcc
    11761 tgctcaccgg gcttcccggc ggggtgatcg gactgctgat cgtggcgaac ctgacgatct
    11821 tcgtcctggg gttcttcatc gacttcttcg agatcgcctt catagtgatc ccgctcctgg
    11881 cgcccgccgc ctacgcgctg ggcatcgacc tggtgtggtt cggggtgatg atcgggatga
    11941 acctgcagat gtcgttcctg acgccgccct tcggattcgc cctgttctac ctgaggggtg
    12001 tcgcgccgcc cggcatcacc acggcccaga tctaccgggg ggcggtgccg ttcatcgcga
    12061 tccaggcggt ggggctggtg ctgatcattc tcttccctgg cctggtcacc tggcccttct
    12121 gaggacgtcg attcgtcgcg tttgctggtc gcgcagatga ggttcgggcg cggccggtac
    12181 ggttggctgg cggagaatgg cccggatgtc gtgaaatgga ctacgtgcgg cagggatttt
    12241 gctctacact cgaataatgc acggccccca cacgaaatgg ccgaataaaa ttggacaggg
    12301 gcacccggtg atcgtatgct ttgcgtggac cagtaccgga cgtccatgct cgaagccctg
    12361 gaccgcaccg atcgccgcat cctgaacatc ctgcagtcgg acggcggcat caccaacgtg
    12421 gagctctcgc gccaggtggg cctggccccg gcgaccacgc tcgacagggt gcgcaagctc
    12481 cggcagaacg ggttcatcct cgggtacgtc gcgctcgtgg actcggccat ggtggaacag
    12541 agcacggtgg cgttcgtggc cgtatcgctc acggagcacg gcgccgagaa ggtggaggcc
    12601 ttccgggccc acatcgagtc gctgcccgag gtgctggaat gctaccacgt ctcgggcgag
    12661 agcgacttcc tgctgaagat cgtggcgcgg gacatccgga cgtacgagga cttcctgctc
    12721 caccggctga cctcgatccc gtgcctgggt cgcatccaca cctccttcgt gctctcgacc
    12781 gtgaagcacg acaccaggat ccccatcgac gaatcctgac cggccccgcc ggcatcagcc
    12841 atggacgatt ccgaccgcca ttacgacccc acgacccgtc tcatccacgg acgcatgcga
    12901 tccgaggcct gggaatacgg tcaccacctg gtgccgccga tctcgacgtc gaccacgttc
    12961 cggctcgatt cgacgagtcg cggcgccatg ggcttcgagg agttcggcca tcccgagggg
    13021 cccgaccacc acatctacat ctacgaccgg cttcacgagc ccaacaagga cctgctcgag
    13081 gagcggctcg cggcggcgga agggggcgag gtggccgtga cgttcgcctc ggggatggcc
    13141 gcgatctcgg ccgccctggg agtcctcacg aaggcgggcg accacgtgct cacgcaccgg
    13201 gccatgtacg gctgcacgca ttcgctgctg caccgctggt acccgcgcat gaacgtggcc
    13261 gtctcggacg cggacctgcg cgacgtcgag ggggcggtcc gggagatcag gcccgagacc
    13321 cgcgtcgtgt acctggagac gccggccaat ccgacgatgg acatcatcga cctggcaagc
    13381 ctcgcggcgg agatccatct cctgaacacg aagcgcgacg agtccgagcg gatctggctg
    13441 gtcgtggaca acaccttctc cacgcc
//
LOCUS       DBNHQZ010000374         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1874517, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000374 DBNHQZ010000000
VERSION     DBNHQZ010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1874517"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="AC16YU_10150"
     CDS             <1..1422
                     /locus_tag="AC16YU_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6065630.1"
                     /translation="ISENGFRYALDHPLSTFSIDVDAASYSNMRRFIRQGRVPPVDAV
                     RIEELVNYFSYDYPQPDGDVPFSLLTEVGQAPWNPAHYLVHVGLQGEKIDFVGRPATN
                     LVFLLDVSGSMDSPDKLPLLKKAFRMLAAELSENDHVAIAVYAGAAGLVLPPTPGDRT
                     EVILEAIDRLQAGGSTAGAAGIHLAYDAAREHFVEGGINRVILATDGDFNVGVSSDSE
                     LIRLIEEKRESGVFLTVLGFGTGNLKDNKMEQLADHGNGAYYYIDSELEARKVLVTEL
                     GGTLHTIAKDVKIQVEFNPAIVRAYRLIGYENRLLASEDFNDDTKDAGELGAGHTVTA
                     LYEVIPVGAVTDTLPPEVDSLRYQDGRFTAAAGSGELMHVKLRYKEPAGTVSRLVDAV
                     VRKPGSTGVVSADFAFSAAVAEFGMIVRGSDHRGTATLGQVLELAHAGLGADPEGYRA
                     DFIRLVGEYGSLVDGLEEPTAAR"
     gene            complement(1423..1821)
                     /locus_tag="AC16YU_10155"
     CDS             complement(1423..1821)
                     /locus_tag="AC16YU_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065631.1"
                     /translation="MTPRFGIDTSILVRLVTADPPESFGYCVERLTEVVESEDAEIIA
                     SGHVIGEAYVALQHHFGVSKPEAKAGLLGALTSGLVAPLQRDETLSALAARGGSGLVD
                     RLIAIDYRRADLDTLTLDRRMAKLENSRLL"
     gene            complement(1799..2041)
                     /locus_tag="AC16YU_10160"
     CDS             complement(1799..2041)
                     /locus_tag="AC16YU_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR6065632.1"
                     /translation="MILKITSKRQVTFPKSVLDALGVGPGDRLELVEEGSGYVIRPRR
                     VDRSKLGMLKNRLRRGVGTFDLDTFREEPHDPSLRD"
BASE COUNT          396 a          784 c          786 g          356 t
ORIGIN      
        1 atctccgaaa acgggttccg gtacgcgttg gaccacccgc tttcgacctt ctcgatcgat
       61 gtggacgccg cctcgtacag caacatgcgc cggttcatcc ggcagggccg ggtgccgccg
      121 gtcgacgccg tgcgcatcga agagctcgtc aactacttct cgtacgacta tccgcagccc
      181 gacggggacg tgcccttctc cctgctgacc gaggtcggac aggcgccctg gaatccggcg
      241 cactacctgg tgcacgtcgg gctccagggc gagaagatcg acttcgtcgg ccgtccggcg
      301 accaaccttg tgttcctgct ggacgtctcc ggttcgatgg actcgcccga caagctcccg
      361 ctcctgaaga aggccttccg gatgctggcg gccgagctct cggagaacga ccacgtcgcg
      421 atcgccgtct acgccggggc ggcgggcctg gtgcttcctc ccacgccggg cgaccggacg
      481 gaggttatcc tcgaggcaat cgaccggctc caggccggtg gctcgacggc cggtgcggcg
      541 ggcatccacc tggcgtacga cgcggcccgc gagcacttcg tggagggcgg catcaaccgg
      601 gtgatcctcg cgaccgacgg cgacttcaac gtgggcgtct ccagcgattc ggagctcatc
      661 cggctcatcg aggagaagcg cgagtcgggc gtcttcctga ccgtcctggg cttcgggacc
      721 ggcaacctga aggacaacaa gatggagcag ctcgccgacc acgggaacgg cgcctattac
      781 tacatagact cggagctcga ggcccgcaag gtgctggtga ccgagctcgg cgggacgctg
      841 cacaccatcg cgaaggacgt caagatccag gtcgagttca acccggccat cgtccgcgcc
      901 taccggctga tcgggtacga gaaccgcctc ctggcctccg aggacttcaa cgacgacacc
      961 aaggacgctg gtgaactcgg cgccggtcac accgtgaccg cgctctacga ggtgatcccc
     1021 gtcggcgccg tgaccgacac gctgccgccc gaggtggaca gcctgcggta ccaggacggc
     1081 cgcttcaccg ccgcggccgg ctccggcgag ctcatgcacg tgaagctccg ctacaaggag
     1141 cccgccggca cggtgagccg gctcgtggac gcggtggtcc ggaagcccgg atcgaccggc
     1201 gtcgtgagcg cggacttcgc cttctcggcc gccgtggccg agtttgggat gatcgtgcgc
     1261 gggtccgacc accgcggcac ggcgacgctc gggcaggtgc tcgagctggc gcatgccggc
     1321 ctcggcgcgg atcccgaggg ctaccgggcc gacttcatcc gcctcgtggg ggagtatggg
     1381 tccctggtgg acgggctgga ggagccgacc gctgcacgct gatcagagaa gccgcgagtt
     1441 ctcgagcttc gccatgcgcc ggtccagcgt gagggtgtcc aggtccgccc ggcggtagtc
     1501 gatggcgatg agccggtcca ccaggccgct gccgccgcgg gcagcgagcg cagaaagcgt
     1561 ctcgtctcgc tggagggggg ccacgagccc gctggtgagg gcgccgagca ggccggcttt
     1621 cgcttccggt ttggagactc cgaagtggtg ctgcagggcg acgtaggcct cgccgatcac
     1681 gtgtcccgag gcgatgatct cggcgtcctc cgactcgacg acttccgtga gccgctcgac
     1741 gcagtaaccg aagctctccg gcggatcggc cgtgaccaga cggaccagga tcgacgtgtc
     1801 aatcccgaag cgaggggtca tggggctcct cgcggaacgt gtccaggtcg aacgtcccga
     1861 ctccacggcg caggcggttc ttcagcatgc cgagcttgga cctgtcgacc cgtcggggcc
     1921 tgatcacgta accgctcccc tcctcgacga gctcgaggcg atccccgggg ccgaccccga
     1981 gggcatccag gacggacttc ggaaaggtca cctggcgttt ggatgtgatc ttgaggatca
     2041 tcgacgcgta aggcactcac cttaccatta tgccgaaaaa gtaaggaggt ttccgggaca
     2101 cgtacatgga gcgcccacgt gtccgggacg taacgaaagg ccctcacgcg ttgtccgagc
     2161 cgatcgccgc ggggatagga agcagtccga cgaccacgaa gaacacgaaa tacgggttga
     2221 acccgctgat ccccagggcc cacgcaccga acaccacgta ggcgccgccg gccagccaga
     2281 ggaccggccg tgaatagggc ccctcccgaa gcgcgcggga cg
//
LOCUS       DBNHQZ010000375         6257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2416559, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000375 DBNHQZ010000000
VERSION     DBNHQZ010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2416559"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="AC16YU_10165"
     CDS             <1..515
                     /locus_tag="AC16YU_10165"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6065633.1"
                     /translation="FSLLVLVAAPAAAQDTRAELASAYSMAESKLLAMAGAVSAEQLA
                     WRPAEGVRSTSEVLNHVAGANYLMASFIGCSAPEGADAPASFEGGPEYEGVSDPAQIK
                     ARLAASVDLLESCAAGLSDEQLAGTMEWFGMSGSGQTFMYFIAGHMHEHVGQLIAYLR
                     MNGVTPPWNS"
     gene            565..1428
                     /locus_tag="AC16YU_10170"
     CDS             565..1428
                     /locus_tag="AC16YU_10170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6065634.1"
                     /translation="MRRILVLAAFATAVLPSARAQSGRVLDALTLSSEILGREMRYAV
                     YLPPDYDTSERSYPVLYLLHGGGDDQAGWIQFGEVLHIADRAVREGLATPMVIVMPDA
                     SGPPRGYFNDPTGSWRFEDFFFDELMPHVESAWRIRAEKRYRAVAGLSMGGGGSFMYA
                     LHRPDLFSAAAPLSASTGPRSVEEMVQRLSPEARALGTERLEEYFRRHSTLALVESLP
                     QDELRSVRWYIDCGDEDFLFEGNALVHIALRKKEVPHEYRVRDGGHTWTYWRTALPDV
                     LAFVSDGFHQH"
     gene            1630..2577
                     /locus_tag="AC16YU_10175"
     CDS             1630..2577
                     /locus_tag="AC16YU_10175"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR6065635.1"
                     /translation="MRFATPALAALLLAFPAAAQEQVTLSPIRDTSIYEESGGLGNGA
                     GAYLFAGETSTGSERRALLAFDVSPIPSSATITGVSLTLHMSKTATGAQTVRLHRLLQ
                     DWGEGTSNADANEGQGAAATTGDATWTDRFFGTSTWDTPGGVFETFSSANVQVGGIGS
                     YTWAVEPMIADVQGWVDGTHGNFGWIVVGASGLKTAKRFDSRENPTEAFRPVLTVTYT
                     TGTAVEDTPAAAGPTLAQNWPNPFGARTEIAFTVAAASHVRLEVYDLLGRRVATLVDA
                     RLPAGTHTASFEAAGEPAGIYLARLQAGERVLVRQMARM"
     gene            2802..5396
                     /locus_tag="AC16YU_10180"
     CDS             2802..5396
                     /locus_tag="AC16YU_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR6065636.1"
                     /translation="MTRSILLLVLLAGCSTGPDYLDPDLPVDRRVDDLVGRMTLEEKV
                     LQMNDQAPAIERLGVPEYNWWNEALHGVARSGLATSFPQAVGIAATWDEDLVFRMATA
                     ISDEARAKHHEYVRRGSRLRYQGLTIWSPNINIFRDPRWGRGQETYGEDPYLTGRIAV
                     PFIRGLQGDDPDYLKTVATVKHFAVHSGPEPDRHTFDAVVSERDLRETYLPHFETGIR
                     EAGAYSLMCAYNRVYGDAACASDLLLKDILRGEWGFDGYVVSDCWAINDIWENHKLVD
                     TPEEASAMAVRSGTDLDCGTEAFPSLVAAVEQGLISEAEIDTAVKRLFRARFRLGMFD
                     PPDRVAYARIPYSVVDRPEHRELAREVTRKSMVLLRNDGVLPLSKDLGSIAVIGPNSD
                     EWLMLLGNYNGTPSEVVTPLRGIREAVSPGTRVLFSRGSDPADGIPLYEIVPAEALAT
                     PDGEPGLVAEYFGDRDRTGDAALSETVPTLDENWADGAPSEELDGDDFGARWTGVLRP
                     GRSGTYRLGVIATMAFEVTFADSVLVASRYNYRNELGDPRVVAAPPLELEAGREYPIV
                     VEASESYGDAMMQLVWAAPSDGLLTEAVAAAREAEAVVLFLGLTPRLEGEEMRVEVEG
                     FTGGDRTDLVLPAAQQRLLEAVTLAGKPTVLVLLGGSALAVGWADRNVGAIVEAWYPG
                     QAAGTAIADVLFGDYNPAGRLPVTFYRSVGDLPPFEDYAMTGRTYRFFEGEPLYPFGH
                     GLSYTSFGYENLEFSAETLGQGGSLTVSVDVTNTGERAGEEVVQLYVRRPESAVDRPI
                     RDLRGFRRVALGPGETTTVAMELEADALRHWDPDADAWALEAGPVLVQVGASSADIRV
                     EGTVRVAE"
     gene            5516..>6257
                     /locus_tag="AC16YU_10185"
     CDS             5516..>6257
                     /locus_tag="AC16YU_10185"
                     /inference="COORDINATES: protein motif:HMM:NF020337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR6065637.1"
                     /translation="MLLLWGLGAPLRAPGQAFESSNLPIVVLQVAQPIPNEPKVAGHM
                     GVVDNGPGVRNRPTDPFNAYDGPIGIEVRGSSSQSFPKKSYAVETRDALGEGLSVPLL
                     GMPEEEDWVLYGPYSDKTFLRNALIMHLSRRIGRYASRTRFVELVLNGSYQGVYVVME
                     RIKRDPNRVDIARLDPDEMTGEDVTGGYILKIDKTDGAQVDGWLSSFPPYPGSPNRPY
                     FQYHYPRPEDIVPEQEAYIQDAVYAFESR"
BASE COUNT          885 a         2169 c         2275 g          928 t
ORIGIN      
        1 tgttctccct cctggtcctc gtggccgcgc cggccgcggc ccaggacacg cgcgccgagc
       61 tcgcctccgc ctactccatg gccgagtcca agctgctggc gatggccggc gccgtctccg
      121 ccgagcagct ggcctggcgt ccggccgagg gcgtgcgctc gacctccgag gtgctcaacc
      181 acgtggccgg cgcgaactac ctgatggcct cgttcatcgg ctgctccgcc ccggagggcg
      241 ccgacgcgcc cgcctcgttc gagggcggtc cggagtacga gggcgtctcg gaccccgcgc
      301 agatcaaggc ccggctcgcg gcgtcggtcg accttctcga gtcgtgcgcg gccggcctct
      361 cggacgagca gctcgccggg accatggagt ggttcgggat gtccggatcg ggccagacgt
      421 tcatgtactt catcgccggt cacatgcacg agcacgtggg gcagctgatc gcgtacctgc
      481 gcatgaacgg cgtcacgccg ccgtggaact cgtaggattc cgttcgtttc gagaggtcgt
      541 cccgttcacc gacgatccct gccgatgcgc cggatcctgg tccttgcggc gttcgccacc
      601 gcggtccttc cctcggcccg tgcccagtcg ggccgcgtcc tggacgcgct gaccctgtcc
      661 agcgagatcc tgggccggga gatgcgctac gccgtgtacc tcccgccgga ctacgacacg
      721 tccgagcgga gctatcccgt gctgtacctg ctgcacgggg gaggggacga ccaggccggc
      781 tggatccagt tcggcgaggt gctgcacatc gcagaccgcg cggtccgcga ggggctcgcg
      841 acgccgatgg tgatcgtcat gccggacgcg agcggcccgc ccaggggcta cttcaacgac
      901 ccgacgggct cgtggcgctt cgaggacttc ttcttcgacg agctcatgcc gcacgtggag
      961 tcggcgtggc gcatccgcgc ggagaagcgc taccgggccg tcgcgggcct ctcgatgggc
     1021 ggagggggct cgttcatgta cgcgctgcac cggccggacc tcttctcggc ggccgccccg
     1081 ctctccgcgt ccacgggtcc gcgctcggtg gaggagatgg tccagcgcct gagcccggag
     1141 gcccgggccc tcggcaccga gcgcctcgag gagtacttcc ggcgtcacag cacgctcgcg
     1201 ctcgtcgagt ccctgccgca ggacgagctc cgctccgtac gctggtacat cgactgcggc
     1261 gacgaggact tcctgttcga ggggaacgcg ctcgtgcaca tcgccctccg gaagaaggag
     1321 gtgccgcacg agtaccgggt gcgcgacggc ggccacacgt ggacctactg gcgaacggcg
     1381 ctgcccgacg tcctggcgtt cgtctcggac ggcttccacc agcactgagc cggtcggcgg
     1441 cgggcacggt ccggcccccg cggtgcacgg tccgactgca ggatccggtc cattcgaccc
     1501 gcgttcgcgg ccggtcgggc agattcggcg cgcggtttcg tccgccgcgc cgtatctgcg
     1561 ccggcgcggc ccggtcggtg gccgacttgg cgccgagggc ccgcgccgtc cacccaatcc
     1621 atacggtcca tgcgcttcgc tacccccgcg ctggccgccc tgctgctcgc ctttccggcg
     1681 gcggcgcagg agcaggtcac gctcagtccc atccgcgaca cgtccatcta cgaggaatcc
     1741 ggcgggctcg gcaacggggc cggcgcgtat ctcttcgcgg gggagacctc gactggcagc
     1801 gagcggaggg cgctcctcgc cttcgacgtc tcgccgattc cgtcgtcggc cacgatcacg
     1861 ggcgtctcgc tcacgctcca catgtccaag acggccaccg gcgcccagac ggtccggctg
     1921 caccggctgc tccaggactg gggggaaggg acgtccaatg ccgacgccaa cgagggacag
     1981 ggcgcggcgg ctacgacggg cgacgcgacc tggacggacc gcttcttcgg cacttccacc
     2041 tgggacactc cgggcggcgt gttcgagacc ttctcgagcg ccaacgtgca ggtgggcggg
     2101 atcggctcgt atacgtgggc ggtcgagccc atgatcgccg acgtgcaggg ctgggtagac
     2161 ggaacgcatg gcaatttcgg ctggatagtg gtaggcgcgt cgggcctgaa gaccgccaag
     2221 cggttcgaca gccgcgagaa tcccacggaa gccttccggc cggtcctcac ggtgacctac
     2281 acgaccggca ccgcggtcga ggacacgccg gcggccgccg ggccgaccct ggcgcagaac
     2341 tggccgaacc cgttcggggc gcggacggag atcgcgttca ccgtcgcggc ggcgtcgcac
     2401 gtacggctcg aggtgtacga cctcctgggc cgccgcgtgg cgacgctcgt cgacgcgcgg
     2461 cttcccgccg ggacccacac ggcctcgttc gaggcggcgg gcgagccggc ggggatctac
     2521 ctggcgcgcc tccaggccgg cgagcgggta ctcgtgcgcc agatggcccg gatgtagccg
     2581 gagccgcgcg gaggtccgcc gggcggagcg ccggccgccg ggccgtgctg cggccctgcg
     2641 cgcggcgagc gtccggatcc gggcccggcg agcggccttc cgcgagtatt cgcacggccg
     2701 gcggtggcgc ggagcgcgaa aatgtccacg cggggtgata ctcttggcgc ggacgccgtc
     2761 gtgacgaagg cggccgcaac cgcagccatc cggaccgcgc catgacccga tcgatcctcc
     2821 tcctcgtcct cctcgccggc tgcagcaccg gccccgatta cctggacccc gacctgccgg
     2881 tcgaccgcag ggtggacgac ctggtcgggc gcatgacgct cgaggagaag gtccttcaga
     2941 tgaacgacca ggcgccggcg atcgagcggc tgggggtccc cgagtacaac tggtggaacg
     3001 aggcgctgca cggggttgcg cggtccgggc tcgcgacgtc ctttccgcag gccgtcggga
     3061 tcgcggcgac atgggacgag gacctggtct tccgcatggc gactgcgata tcggacgagg
     3121 cgcgcgccaa gcaccacgag tacgtccggc gcggttcgcg cctgcgctac caggggctca
     3181 cgatctggtc cccgaacatc aacatattcc gcgacccgcg ctgggggcgc ggccaggaga
     3241 cctacggcga ggatccgtac ctcacgggcc ggatcgccgt gcccttcatc cgcgggctcc
     3301 agggcgacga cccggactac ctgaagacgg tcgccaccgt caagcatttc gccgtgcaca
     3361 gcggccccga gccggaccgt cacacgttcg acgccgtggt ctcggagcgc gacctgcgcg
     3421 agacctacct gccgcacttc gagacgggca tccgggaggc cggggcgtac tcgctgatgt
     3481 gcgcctacaa ccgggtctac ggcgatgcgg cgtgcgcgag cgacctgctg ctgaaggaca
     3541 tcctgcgcgg cgagtggggt ttcgacggct acgtggtctc ggactgctgg gccatcaacg
     3601 acatctggga gaaccacaag ctcgtcgata cgccggagga ggcctccgcg atggccgtcc
     3661 ggagcgggac cgatttggac tgcggcaccg aggcctttcc ctcgctcgtg gcagccgtcg
     3721 agcaggggct catctcagag gccgagatcg acaccgcggt gaagcggctc ttccgcgcgc
     3781 gcttccgcct gggcatgttc gacccgccgg accgcgtggc gtacgcgcgc atcccgtact
     3841 cggtcgtgga tcgtcccgag caccgcgagc tcgcgcgcga ggtcacgcgc aagtcgatgg
     3901 tcctgctccg gaacgacggc gtgctgcccc tctcgaagga cctgggatcg atcgccgtca
     3961 tcgggccgaa ctcggacgag tggctcatgc tgctcggcaa ctacaacggg acgccgtccg
     4021 aggtcgtcac cccgctccgg ggcatccgcg aggccgtttc gcccggcacg cgggtcctgt
     4081 tctcgcgggg gtcggatccg gcagacggca ttccgctcta cgaaatcgtc ccggcggagg
     4141 cgctggcgac tccggacggc gagcccgggc tcgtggccga gtacttcggc gaccgggacc
     4201 ggacgggcga cgcggcgctc tcggagaccg tcccgaccct ggacgagaac tgggcggacg
     4261 gcgccccgtc ggaggagctg gacggggacg acttcggggc gcggtggacg ggcgtcctcc
     4321 ggcccggcag gtcggggaca taccggctcg gcgtcatcgc gaccatggcc ttcgaggtca
     4381 cgttcgccga cagcgtgctc gtcgcctccc ggtacaatta ccgcaacgag ctgggtgatc
     4441 cgcgcgtcgt ggccgcgccg cccctggagc tcgaggcggg ccgcgaatac cccatcgtcg
     4501 tggaggcttc ggagtcgtac ggcgacgcga tgatgcagct ggtctgggcc gcgccctcgg
     4561 acggcctgct gaccgaggcg gtcgcggccg cccgcgaggc cgaggccgtg gtgctgttcc
     4621 tgggcctgac gccgcggctc gagggggagg agatgcgggt cgaggtggag ggattcacgg
     4681 gaggcgaccg caccgacctg gtgctgcccg cggcgcagca gcggctgctc gaggccgtga
     4741 cgctcgccgg caagcccacg gtgctcgtgc tgctcggcgg cagcgcgctc gcggtcggat
     4801 gggccgaccg gaacgtgggc gcgatcgtgg aggcctggta cccggggcag gcggcgggga
     4861 cggcgatcgc cgacgtgctg ttcggggact acaatccggc cggccggctg ccggtcacct
     4921 tctaccggag cgtgggcgac ctgccgccct tcgaagacta cgccatgacg gggcggacat
     4981 accgcttctt cgaaggcgag ccgctctacc ccttcgggca cggactgagc tacacgagtt
     5041 tcggatatga aaacctggaa ttcagcgcgg agaccctcgg gcagggcgga agcctcacgg
     5101 tgagcgtcga cgtgacgaac accggggagc gggccgggga ggaggtcgtg cagctctacg
     5161 tgcggcgacc cgagtcggcc gtcgaccgcc cgatccgcga cctgcgcggc ttccggcgcg
     5221 tggcgctcgg gccgggcgag acgacgacgg tcgccatgga gctcgaggcg gacgcccttc
     5281 gccactggga cccggacgcg gacgcgtggg ccctggaggc ggggccagtc cttgtgcagg
     5341 tgggcgcgtc gtcggcggac atccgggtgg agggcacggt ccgggtcgcg gagtgagcgc
     5401 gccaggggaa cgatccgcac ggcgggtgga ttgcatcgat atgcgacgga acggacggaa
     5461 atggcggacg gcggggcttc ccgggcgatc gggggtggtc ccgttcctgg cggcagtgct
     5521 tcttctctgg gggctcggcg cgccgcttcg ggccccgggg caggctttcg agtcgtcgaa
     5581 cctgcccatc gtggtgctcc aggtggcgca gcccatcccg aacgagccca aggtggcggg
     5641 ccacatgggc gtcgtcgaca acgggccggg tgtgcgcaac cgcccgacgg atcccttcaa
     5701 cgcgtatgac ggaccgatcg gcatcgaggt gcggggctcg tcgtcgcagt ccttccccaa
     5761 gaagtcgtac gccgtcgaga cgcgggacgc gctcggggag ggcctttcgg tgccgctcct
     5821 gggcatgccg gaggaggagg actgggtgct ttacggcccg tattccgaca agaccttcct
     5881 gcgcaacgcg ctcatcatgc acctctctcg ccggatcggc cgctacgcca gccggacccg
     5941 cttcgtggag ctggtgctga acggctcgta ccagggcgtc tacgtggtca tggagcgcat
     6001 caagcgggac ccgaaccggg tggacattgc gcgcctcgac ccggacgaga tgacgggcga
     6061 ggacgtgacg ggcgggtaca tcctcaagat cgacaagacg gacggggcgc aggtggacgg
     6121 ctggctttcc tcgttcccgc cctacccggg atcgccgaac cgaccgtatt tccagtacca
     6181 ctacccgcgg cccgaagaca tcgtgcccga gcaggaggcc tacatccagg acgccgtcta
     6241 cgcgttcgag tcgcgca
//
LOCUS       DBNHQZ010000376         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_384347, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000376 DBNHQZ010000000
VERSION     DBNHQZ010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_384347"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..856
                     /locus_tag="AC16YU_10190"
     CDS             197..856
                     /locus_tag="AC16YU_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065638.1"
                     /translation="MRASVRTSVLTLLLAGAVALPGSAQTGVALRVGTLGPGIEVSRR
                     LAPVVSARAQFSYLPRTHVPEYTLNRTDVDVAFDGHATLRSWALFVDLHPPIGPIRVT
                     TGLVSIGSLVEGRGRPLNDYEYQGKVYTPEEMGTVTSEISWKSRVRPYLGFGTGSPPA
                     SRGIGFQFDLGVVFSGAPDFDLTSATSFIPTPAQIERAEEIVRWAKIYPVLSVGVSFG
                     I"
     gene            complement(866..1675)
                     /locus_tag="AC16YU_10195"
     CDS             complement(866..1675)
                     /locus_tag="AC16YU_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065639.1"
                     /translation="MSEEPDRYLLAAFLPDDEAEELERAARGDDEATGMIRASHALRA
                     MIGDVAALRSDAASEPALAFLAAARLAEGSADREWKDLLDRLERRVTERPESLAVYRR
                     LQERIRQLEQGSDAEAHFRRISGTDPPRIRAIRPFLAAAAVLLIVATAGVLGRVLEDP
                     VARAAHESVRRTARAAPSFRGAPGPSPEARRRAADARRLVLGLWPVYDRAALADLARD
                     PEDSLAPVDWLLVGQARLMLGDRRDAEEALRRVPGPDPEAARLLELIAEGD"
     gene            complement(1699..2361)
                     /locus_tag="AC16YU_10200"
     CDS             complement(1699..2361)
                     /locus_tag="AC16YU_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6065640.1"
                     /translation="MHQSELLGIDRLARRLPFALDAVDEAGAAFARWQRSGRPRDRRT
                     VDLWTYCYIRRYFLVRFAREPSFGVSELEDVVEKAFRRVEEGRASLREPERYARWTTV
                     VCRRTYLNFVSRRRELVPVDRIAEPEADGPSAETLHDRAGTMRVLVAAVERLPAFLRP
                     YARLRFVDGLEYEEIARRTGRPVPTVRAYAHKAILRFRADGRLMRHVRERAGYESGEG
                     GS"
     gene            2647..3090
                     /gene="rplM"
                     /locus_tag="AC16YU_10205"
     CDS             2647..3090
                     /gene="rplM"
                     /locus_tag="AC16YU_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6065641.1"
                     /translation="MDVTSFKTKSARGADVERTWYVVDAENQVVGRLASRVASILRGK
                     HKPSYTPHVDTGDYVIVINAEKARFTGKKETDKEYFSHSGYPGGVRIRTPRVQRERKP
                     TFIIENAVKGMLPKGALGREMFRKLKTYAGPDHPHEAQQPQALTL"
BASE COUNT          457 a         1120 c         1081 g          450 t
ORIGIN      
        1 cctcgccgaa ccgtgccggc gtcgtgacgc cgctcgagca gcgcgacggc gagagcatga
       61 tgatgctcat catgcccgtc atgctgaata cgtacgcctg aggccgcctg cggcgcgggg
      121 ctcccgggct gccctttagg atttggccgg tccgcccgat gcttggggtg atccactcca
      181 tcagatcggt gccgacatgc gcgcctctgt caggacctcg gtcctcaccc tccttctcgc
      241 gggcgccgtg gccctgcccg gctcggccca gaccggcgtg gccctccgcg tgggcacgct
      301 cggtcccggc atcgaggtgt cccgccgcct ggccccggtc gtgtccgccc gggcgcagtt
      361 ttcgtacctg ccgcgtactc acgtgcccga gtacaccctg aaccggaccg acgtggacgt
      421 agccttcgac gggcacgcga cgctccggtc gtgggcgctg ttcgtggacc tccatccgcc
      481 gatcggaccg atccgggtca cgacggggct cgtcagcatc gggtcgctcg tcgagggtcg
      541 cggcaggccg ctcaacgact acgagtacca gggcaaggtg tacacgcccg aggagatggg
      601 gacggtgacg tccgagatct cgtggaagtc gcgggtccgt ccgtacctgg gtttcggcac
      661 cgggtcgcct cccgcctcgc gcggcatcgg attccagttc gacctgggcg tcgtcttctc
      721 cggagcgccc gacttcgatc tgacctccgc cacgtcgttc atcccgaccc cggcccagat
      781 cgagcgggcc gaggagatcg tgcggtgggc gaagatctac ccggtgctgt cggtcggcgt
      841 ctccttcgga atctgaccgc tccggtcagt cgccctcggc gatgagctcc aggagccggg
      901 ccgcttccgg atccgggccg gggacccggc gaagcgcctc ttccgcgtcc cggcggtcgc
      961 cgagcatgag ccgggcctgg ccgacgagaa gccagtcgac gggcgcgagc gaatcctcgg
     1021 gatcgcgggc aaggtccgca agcgccgcgc ggtcgtagac gggccagagt cccaggacca
     1081 gccggcgtgc gtccgccgcg cggcgacggg cctccggcga cgggccgggc gcaccgcgga
     1141 acgagggcgc ggcgcgggcc gtgcgccgca cggactcgtg ggcggccctc gcgacgggat
     1201 cctcgaggac gcggccgagc accccggccg tggccacgat gagcagcacc gccgcggctg
     1261 caaggaatgg acggatcgct cggatgcgcg gcgggtccgt gcccgagatg cggcggaaat
     1321 gcgcttcggc gtcggacccc tgctccagct gccggatccg ctcctggagg cgccggtaga
     1381 cggccagcga ttccggccgc tccgtcaccc gccgctccag gcggtcgagc aggtccttcc
     1441 actcgcggtc cgccgacccc tccgcgagcc gggcggccgc gaggaaggcc agcgccggct
     1501 ccgaggcggc gtccgagcgc agcgcggcca cgtcgccgat catcgcccgg agcgcgtgcg
     1561 aggcgcgtat catcccggtc gcctcgtcgt cgcctcgggc cgcgcgttcg agctcttccg
     1621 cctcgtcgtc gggcaggaac gcggcgagca ggtatcggtc tggttcttcg gacatgcgct
     1681 caagtgtagt catctcccct atgacccgcc ctccccggat tcataccctg cccgctcgcg
     1741 gacgtggcgc atgagtcggc cgtcggcacg gaatcggagg atggccttgt gcgcgtaggc
     1801 ccggacggtg ggcaccggac ggccggtcct gcgagcgatc tcctcgtact ccaggccgtc
     1861 cacgaaccgc agccgggcat agggccgcag aaaggccggc aggcgctcga ccgccgccac
     1921 gagcacccgc atagtcccgg cgcggtcgtg cagcgtctcg gcggacggcc cgtcggcctc
     1981 cggctccgcg atccggtcca caggcacgag ctcgcggcga cggctcacga agttcaggta
     2041 ggtcctccgg cagaccacgg tcgtccagcg ggcgtaccgc tccggctcgc gcagcgacgc
     2101 gcgtccctcc tccacccgcc ggaacgcctt ctcgaccacg tcctcgagct cggacacccc
     2161 gaaggacggc tcccgtgcga accggaccag gaagtagcgg cggatgtagc agtacgtcca
     2221 gagatccacc gtgcgccggt cgcgcgggcg acccgaccgc tgccagcggg cgaacgccgc
     2281 gcccgcctcg tccacggcgt cgagcgcgaa gggaaggcgc cgcgcgaggc ggtcgatgcc
     2341 gaggagctcg gactggtgca tgccggttct gggggtaggc gtggccggga gatggaggag
     2401 gctcccgggc gccgtcgcgt aacggccgcg tcgtatcaag aatagtggaa ttgcggcgtc
     2461 cggcataaac caaccgggcg gctccgcgtg cgaggggacc gcgcgatccc gcgccgacgc
     2521 gcctgacgga ttggagaggt tgacggtgcg gggtcgtgcg gcgtatttta gtggactgcg
     2581 gcatacccac ccgccgcaga ccatcgagca ttcgatgccg ggctccccgt tccggccgcc
     2641 atcatcatgg acgtcacttc cttcaagacg aaaagcgcac gcggcgccga cgtcgagcgt
     2701 acgtggtacg tcgtcgacgc cgagaaccag gtcgtgggcc gcctcgcgtc gcgcgtggcc
     2761 tctatcctgc gcggcaagca caagccctcg tacacccccc acgtggacac gggagattac
     2821 gtgatcgtga tcaacgccga gaaggcgcgc ttcacgggca agaaggagac cgacaaggaa
     2881 tacttcagtc acagtggcta cccgggtggc gtccggatcc gcactccgcg cgtgcagcgc
     2941 gagcgcaagc ccacgttcat catcgagaat gccgtcaagg gcatgctccc caagggcgcc
     3001 ctcggacgcg agatgttccg caagctcaag acctatgccg gtccggacca tccgcacgag
     3061 gcgcagcagc cccaggccct gacgctgtag cacgtatccg ctcgcacc
//
LOCUS       DBNHQZ010000377         4357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2820891, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000377 DBNHQZ010000000
VERSION     DBNHQZ010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2820891"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..955
                     /locus_tag="AC16YU_10210"
     CDS             203..955
                     /locus_tag="AC16YU_10210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6065642.1"
                     /translation="MRFALLKIAAVLLGALSAGSGCGADRGAGEDTLHYRSGTPEAED
                     GTGRFYMGREIARGLETEHRIDRPDSPDRDVRDLPDRLVAALELKPADFVADIGSGAG
                     YYTLRLARQVPHGRVFAVDLQPALLDSVVAMAGAEGLRNVEPVLGTEADPSLPAERID
                     LALIVVSYHEFTHPREMIEGIVRALRPGGRLAIVEYRLEDATIPVLDVHRMSAGQIRA
                     EMEAAGLEYRDGRDVLPQQHLLFFRKPFGPRQ"
     gene            952..1632
                     /locus_tag="AC16YU_10215"
     CDS             952..1632
                     /locus_tag="AC16YU_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6065643.1"
                     /translation="MSADWVVELPALFVLGLFGGAHCIGMCGGFVLIAHGAGASSRGA
                     ARLAAYLSGKTATYAVLGVLAGALGHLVGGVSGAQTALSIAVGVFLVLIGLGAAGMIP
                     ERLPGFSVAGPWIGRALGRIVRSGGTRAPLVIGLLNGLLPCGLVYAALAKAATTGSAG
                     GGAAAMAVFGVGTMPALVLTAWLGGQLGPVARRRVARTGGWLVVAFGVYTLWRTFARM
                     GGAMPAHG"
     gene            complement(1640..2134)
                     /locus_tag="AC16YU_10220"
     CDS             complement(1640..2134)
                     /locus_tag="AC16YU_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065644.1"
                     /translation="MSDPAIPRIHSPRDGAAVDGLACTLSWDPVPGATYRVQVSANER
                     FDALLVDADTDNATVLTLYELLPRDGRRLHWRVAATWSGSDGAWSEPTWFLSADIEDR
                     PPAPRAALARAKTAPRSAPPPTVAATAEPVPPYLEETTGAREVLIAFAVLLLTLFLFL
                     LAAY"
     gene            complement(2127..2336)
                     /gene="ccoS"
                     /locus_tag="AC16YU_10225"
     CDS             complement(2127..2336)
                     /gene="ccoS"
                     /locus_tag="AC16YU_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HIR6065645.1"
                     /translation="MNILYLLVPAAVLLAAIGVAAFAWAVRTGQFDDVDTPPVRAILD
                     EEPAGPSADAAKKPSNDSKPTLDDV"
     gene            complement(2333..>4357)
                     /locus_tag="AC16YU_10230"
     CDS             complement(2333..>4357)
                     /locus_tag="AC16YU_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6065646.1"
                     /translation="SAHPRRADGESERSDEERRLLLRMGAAWSLAANVMLIALAFYAG
                     IDRAESPGLFAAGRWVSLVLAVPAIGWAGSVFFRRAWASVRRAVRDRSLRGLDMDTPI
                     ALGLAVGFVDSARATVTGAGEVWFDSLTLLVAALLTARFLQVRSRRLAGDASDRLLSL
                     VPTMARRVAADGTEQHVVADELAVDDRVRVHPGEVVPVDGTVAEGESRLDNAVVTGES
                     RPEAVSPGTKVLAGSTNVSSVIDVVVEAAGDATRVGRLLAWVRDASSRRAPVVQLADR
                     LSGYFVATVLVLAAVTFAAWSLLDPGRAVSHVVALLVVTCPCALGMATPLAMAVAAGR
                     AARSGLFVKSEAALELLTRADTVVLDKTGTLTVGRPAVMSVAGDEQAVILAAAVEAHI
                     PHPIARAFLLHADRRITASDVRIEPGSGVEGMVDGRRVLAGRPDWVSSRTAPLPPGLA
                     DAVRSAAALARTPVAVAVDGSPVAVVSLGDAVRPDARPLLDALRASGVDVEVLSGDHR
                     AVVADMGRVLGLPADAVRGGVSPEDKRDRVNELRAAGRTVVMVGDGVNDAAALAEADV
                     GIAVSGGTSASLVAADIFATGGGLDAIGLLFDSSRRVMRVIRRNLGISLAYNVVVASL
                     AIAGLVDPLVAAVAMPVSSMAVVLSSILQRTFRPRENVESASSVETVPEP"
BASE COUNT          635 a         1551 c         1596 g          575 t
ORIGIN      
        1 cggccagggg gacggattgt ccacaagggg gcgcgcgaga cgggacgcaa taaaccggtc
       61 cacgggatgc ggttcaacgg gggttacaca cagataatcc acatttccat ccacggacgg
      121 ctgaacatcg gactccgtgc cgcgtgcata gcctcacgcc cgccacggcg gccatttcaa
      181 cgacccgttc aaccacaggg ccatgcgatt tgccctgctc aagattgccg ccgtactgct
      241 gggtgcgctc tccgccgggt ccggttgtgg cgcggatcga ggggcgggcg aagacaccct
      301 gcattaccgg tcgggcacgc ccgaggccga agacgggacc gggcggttct acatggggcg
      361 cgagatcgcg cggggcctgg aaacggagca tcgcatcgac cggccggaca gtcccgaccg
      421 ggacgtgcgc gaccttcccg atcgcctcgt cgccgcgctg gaactcaagc cggcggactt
      481 cgtggccgac atcggctcgg gcgccggcta ctacacgctg cgcctggccc gccaggtgcc
      541 ccacggtcgc gtgtttgccg tggacctgca gcctgccctg ctcgattccg tggtggcgat
      601 ggccggcgcg gagggacttc ggaacgtgga gccggtcctc gggaccgaag cggatccgtc
      661 cctgcccgcg gagcgcatcg acctggccct catcgtggtc tcgtaccacg aattcactca
      721 cccacgcgag atgatcgagg gcatcgtgcg cgcgctgcgg ccgggcggtc gcctggcgat
      781 cgtcgagtat cggctggagg atgccacgat tccggtgctg gacgtccacc gtatgtcggc
      841 cgggcagatc cgtgccgaaa tggaggcggc cggcctcgaa taccgcgacg gccgggacgt
      901 gcttccccag cagcacctgc tgttcttccg caagcccttc gggccccggc aatgagcgcg
      961 gactgggtgg tggagctgcc ggcgctcttc gtgctcggac tcttcggcgg ggcgcactgc
     1021 atcggcatgt gcggcggctt cgtgctgatc gcccacggcg cgggtgcttc gtcgcgcggc
     1081 gccgcccgcc tcgcggccta cctttcgggc aagaccgcca cgtacgccgt gctcggcgtt
     1141 ctggccggcg ccctcggtca cctggtcggc ggcgtgtcgg gcgcgcagac ggccctctcc
     1201 atcgccgtcg gcgtgtttct cgttctcatc ggcctcggtg cggccgggat gatccccgag
     1261 cgccttccgg ggttcagcgt ggccggacca tggatcggga gggcgctcgg gcggatcgtc
     1321 cgttccggag gcacccgcgc gccgctcgtg atcgggctgc tgaacggtct cctgccttgc
     1381 gggctcgtgt acgccgcgct cgccaaggcc gcgacgaccg gctccgccgg gggcggggcc
     1441 gcggcgatgg ccgtcttcgg cgtgggcacg atgccggccc tcgtgctgac ggcctggctg
     1501 ggcggccagc tgggcccggt ggcgcggcgc agggtggccc gcacaggggg ctggctcgtc
     1561 gtggccttcg gcgtgtacac cctctggcgg acgttcgccc gcatgggcgg cgccatgccg
     1621 gcgcatggct gagcccgcct cagtaggccg ccaggagaaa caggaagagc gtcagaagca
     1681 gcacggcgaa cgcgatgagc acctcgcgcg caccggtcgt ctcttccagg taggggggaa
     1741 ccggctcggc cgtcgcggcg acggtcggag gaggcgcgct gcggggcgcg gtcttcgccc
     1801 gggcaagggc ggcccggggc gcgggagggc ggtcctcgat gtcggccgag agaaaccagg
     1861 tcggctcgct ccacgcaccg tccgaacccg accaggtcgc ggcgacgcgc cagtgcagtc
     1921 ggcgaccgtc cctgggcagg agctcgtaca gggtcaggac ggtggcgttg tcggtgtcgg
     1981 cgtcgacgag cagggcatcg aaccgctcgt tcgcggagac ctgcacccgg taggtggcgc
     2041 caggaacggg gtcccaggac agggtgcagg cgaggccgtc gacggccgcg ccgtccctcg
     2101 gggaatgaat gcgggggatt gccggatcag acatcgtcca gggtcggttt cgaatcgttc
     2161 gatggtttct tcgctgcgtc tgcagagggg ccggccggct cctcgtcgag gatcgcgcgg
     2221 acgggggggg tgtccacgtc gtcgaactgc ccggtccgca cggcccacgc gaacgcggcc
     2281 acaccgatcg cggccaacag gaccgccgcc ggcaccagaa ggtacaggat attcatggtt
     2341 cgggaaccgt ttcgacggaa gaagccgact ccacgttctc ccgcggacgg aaggtccgct
     2401 gcaggatgga cgacaggacc acggccatcg agctgacggg catcgcgacg gccgccacga
     2461 gcggatcgac caggccggcg atggccagcg aggccacgac cacgttgtac gcgagcgaga
     2521 tgcccagatt ccgccggatc acgcgcatca cccgccggga cgagtcgaac agcagcccga
     2581 tcgcgtccag cccgccgccc gtggcgaaga tgtcggcggc cacgaggctc gcgctcgtgc
     2641 cgcccgagac ggcgatcccc acgtccgcct cggcgagcgc ggcggcgtcg ttgacgccgt
     2701 ctcccaccat gacgacggtc cggccggctg cccgcagctc gttcacgcgg tcgcgcttgt
     2761 cctcggggct caccccgccc cgcacggcat ccgccggcag tcccaggacc cggcccatgt
     2821 cggctacgac ggcgcgatgg tcaccggaaa gcacctcgac gtcgaccccc gatgcgcgca
     2881 gcgcgtcgag cagcggccgg gcgtccgggc gcacggcatc gcccaggctc acgacggcca
     2941 cgggactgcc gtcgacggcc acggccaccg gcgtccgggc gagggccgcg gcggaccgta
     3001 cggcgtcggc gagcccgggt ggaagtggag ccgttcgcga tgatacccag tcggggcgtc
     3061 cggcgaggac ccggcgcccg tcgaccatgc cctcgacgcc tgagcccggc tcgatgcgga
     3121 cgtccgaggc cgtgatccgc cggtcggcgt ggagcaggaa ggcccgcgcg atggggtggg
     3181 ggatgtgggc ttcgacggca gcggccagga tgaccgcctg ctcgtcgccc gcgacggaca
     3241 tcaccgccgg tcggccgacg gtcagcgtcc ccgtcttgtc cagcaccacc gtgtccgccc
     3301 gcgtgagcag ctcgagggcc gcctcgctct tgacgaacag gccgctacgc gcggcccgcc
     3361 cggcggccac ggccatggcg agcggcgtgg ccatgcccag ggcgcagggg caggtgacga
     3421 cgaggagcgc caccacgtgc gacacggcac gcccgggatc gagcaggctc catgcggcga
     3481 acgtgacggc ggccaggacc aggaccgtcg ccacgaagta gcctgacagc cggtccgcga
     3541 gctggacgac gggagcgcgg cgcgaggagg cgtcccgcac ccaggccaga aggcggccga
     3601 cccgcgtggc atcgccggcg gcctcgacca cgacatcgat tacggacgac acgttggtcg
     3661 agccggccag caccttcgtg ccgggcgaaa cggcctcggg gcgggactcg cccgtgacca
     3721 cggcattgtc caggcgggat tcgccctcgg cgacggtccc gtccacgggg acgacctcgc
     3781 ccggatgcac gcggacccgg tcgtccaccg ccagctcgtc cgccacgacg tgctgctccg
     3841 tgccgtctgc ggccacccgc cgcgccatcg tcggcacgag cgagagcagg cggtccgacg
     3901 cgtcacccgc cagccgccga ctccgcacct ggaggaaccg ggccgtcagg agcgcggcca
     3961 cgagcagggt caggctgtcg aaccagactt cgcccgcgcc ggtcaccgtg gcgcgggcgg
     4021 aatcgacgaa gccgaccgcg aggccgaggg cgatgggagt gtccatgtcc agtccgcgca
     4081 gggaccggtc gcgcacggcc cgccggaccg acgcccaggc ccgtcggaag aacacggagc
     4141 cggcccatcc gatggccggc accgcgagaa cgagactcac ccagcgcccg gcggcgaaga
     4201 gcccgggcga ctcggcccgg tcgatcccgg cgtagaatgc gagcgcgatc agcatgacgt
     4261 tggccgccag cgaccaggcc gcacccatcc gcagcaggag ccgtcgttct tcgtccgacc
     4321 gctcggattc gccatcggcg cgcctgggat gggccga
//
LOCUS       DBNHQZ010000378         6669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_247821, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000378 DBNHQZ010000000
VERSION     DBNHQZ010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_247821"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16YU_10235"
     CDS             <1..195
                     /locus_tag="AC16YU_10235"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6065647.1"
                     /translation="QVDCEWTKSGKVDVIDREVTLFGELDDAQRARLLEIANRCPVHR
                     TIDGGPCVRTYERRPEELGR"
     gene            192..1172
                     /locus_tag="AC16YU_10240"
     CDS             192..1172
                     /locus_tag="AC16YU_10240"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR6065648.1"
                     /translation="MRAIRFDEPGGPDVLYVGTARDPFAGLGEVLVRVEATALNRADL
                     LQRQGRYPVPEGVTSVPGLEMAGTVETVGEGVEGFATGDRVFALLQGGGYAERVVVPS
                     GHLMHVPARLSTAQAAAVPEAFMTAYQALFLLAGLGEGDAVLVHAGASGVGTSAIQLA
                     VARGITVFVTCSADKRGRCEELGAARAIDYRSERFVDAVADATGGRGVRAVIDVVGAP
                     YFEDNLASLSEDGVLVVLATMGGSRVDSLDLRTLFRRRLSVVTSTLRTRSAEYRSELT
                     AGFVRDVIPLLASGKVAPVIDTVLPWTDVARAHRHMEANRNVGKIVLLVD"
     gene            complement(1189..2208)
                     /locus_tag="AC16YU_10245"
     CDS             complement(1189..2208)
                     /locus_tag="AC16YU_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6065649.1"
                     /translation="MTPEVSALPVHLVRPFRIAHGTSTVRTNVLVRLGEAFGEAGLPP
                     YLGTTVDDVAEWMKGVSLPDLPDEGPIPLEHVLEGLPPGPPAARCALDMALHDLWGRR
                     LGYPLYTLFGLDPRCSPVSFVTLSIPEDLDDLARDARSFGPHDRLKLKVGTGNPERDL
                     EVVWTARGATDALIVVDANGAWTRDEAVDMLPALLEAGVSIVEQPIAAGDPDDWYRLR
                     RALGSTRVPALFADESVFTVDDVIALAGAADGINVKLAKAGGIAPARVLIAMARALDL
                     QVLLGCMIESSLAVAAAAHLAPLVDFADLDAPLLVRDDPFDGVEIREGRVLPTDRPGI
                     GAEPV"
     gene            complement(2205..3197)
                     /locus_tag="AC16YU_10250"
     CDS             complement(2205..3197)
                     /locus_tag="AC16YU_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065650.1"
                     /translation="MKKPGTGDPALIRSLVDRVIDELGPGASPEEVRRTAALVLGEIG
                     PGSFPASLAPVILVDQPAAPDSTGGLSAVRERLSGETRLDLWIRTSVDLPVREPRPVA
                     ESVLDSLRAQAERAESGAAGRICLAVGPGSSTMPLDWIPGLLERDSRVRLAVTCARPG
                     LGVDRHLLDRLLRVRSDAPPALAERLFAACMRLPLGRTGRPRIEVLLPWAAVQAEEPA
                     AAVRTSPGLADIRSALAGAADVRAVILVSARASSADWTALRSWRARFGSPPAAVDGLR
                     VEGFPAAGVRPFGDAPLCTSPGQAAAHLASLACGGAVAPLCLSHAGPSGNGAPT"
     gene            complement(3295..4830)
                     /locus_tag="AC16YU_10255"
     CDS             complement(3295..4830)
                     /locus_tag="AC16YU_10255"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR6065651.1"
                     /translation="MSAKGRFSLSLLLLATFAAGILSATVGGNWLGAGDRIGTASYAS
                     QATSESRRPALDPRALAFETAFVDVAEIVNPAVVQIRSEQVREQQSINPFEGSPFEDW
                     FRMPDQQPRTFRTRSLGSGVIVRENGYVVTNYHVIREASDHEVVLSDGTFHKATVVGS
                     DEASDLAVIRIEKQGLPVVPFGSIEDVRPGQWVLAFGSPLAEELGNTVTSGIVSAVGR
                     TSRNLTALNLFASFIQTDAAINPGNSGGPLVDLRGHLIGINSAIYSRSGGYQGIGFAI
                     PVDVVKNVADQLIDSGTVERGFLGVLFDGVSQSLAEAMGVPRGSAQITSVTDGSPAED
                     AGLKAGDIITAVDGHELRSADELRTRVGNMAPGDRVELTLNRDGESRVVRVELGRRSE
                     FVDATAEAAPPGGTQETDELGALGMTLRPLSATAAERLGLPAGTTGILVSAIDTESTA
                     YREADVRQGDVIVEADRRPVTSPEELRSVIADVKSGDSFIVKVLRPQQGQVSTFFTAL
                     TRP"
     gene            complement(5037..6521)
                     /locus_tag="AC16YU_10260"
     CDS             complement(5037..6521)
                     /locus_tag="AC16YU_10260"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6065652.1"
                     /translation="MRRIQDLPDAHLRVFDRPALPDAEAFEDVYLIGICGTGMGSLAG
                     LFHQAGYRVRGSDAAAWPPMSTRLGELGIPVLEGFDAGHLDPRPGLVVVGNACTPTHV
                     EAARARESSLAQASLPEAIAHYFIRDRVSLVVAGTHGKTTTTSLLVHVLRTAGLDPGF
                     LVGGVMRNGDVSYGVGAGSHFVIEGDEYDSAYFDKRPKFLHYRPTHAVVTSLEFDHAD
                     IYDTWSDYRDAFREFARTVNPAGSLVLNADDPEVRALARHTGARVVTYGLAAADLDVT
                     AGGISSEGDGQRFTLVHRGTALGTFFLPMSGRHNLSNALAVCAVALGEGVTPGALREG
                     LATFEGIRRRQEIRGEVDRVTVLDDFAHHPTAVRATIDAVRERWPERRLVAVFEPRSN
                     SSRRKVFEEGYMRAFDRADAVFLSTPPFRHNDRAEDFMDIGRVTASLERRGIHTALGR
                     DADELLPALIDWIRPGDVVLVMSNGGFGNIHARLLAAIAERAPA"
BASE COUNT         1000 a         2272 c         2468 g          929 t
ORIGIN      
        1 caggtcgact gcgagtggac gaagtccggc aaggtcgacg tgatcgatcg cgaggtgacc
       61 ctgttcggcg agctggacga cgcccagcgg gcccgcctgc tcgagatcgc caaccgctgc
      121 cccgtgcatc ggaccatcga cggcggtccg tgcgtgcgga cgtacgagcg tcgcccggag
      181 gagctcggcc ggtgagggcg atccggttcg acgagccggg cggcccggac gtgctgtacg
      241 tcgggacggc gcgggacccg ttcgcggggc tcggcgaggt cctggtccgg gtggaggcta
      301 ccgcgctgaa ccgtgcggat ctcctgcagc gacaggggcg ctacccggtc cccgaggggg
      361 tcacgtcggt ccccgggctc gagatggccg ggacggtcga gacggtgggc gaaggggtgg
      421 agggcttcgc cacgggcgat cgcgtgtttg ccctcctgca gggcgggggg tacgccgaac
      481 gggtcgtcgt cccgtccggg cacctcatgc acgttcccgc gcgcctttcg acggcccagg
      541 cggccgcggt gcccgaggcg ttcatgacgg cgtaccaggc cctcttcctc ctcgcgggcc
      601 tcggagaggg cgacgcggtg ctcgtccacg ccggggcttc gggcgtgggg acctcggcca
      661 tccagctcgc cgtcgcccgg ggcatcacgg tgttcgtcac gtgctcggcc gacaagcggg
      721 ggcgatgcga ggagctcgga gccgcacggg ccatcgacta ccgttcggaa cggttcgtgg
      781 acgcggtggc cgatgcgacc gggggacggg gcgtccgcgc ggtgatcgac gtcgtcgggg
      841 cgccttactt cgaggacaac ctggcgtcgc tctcggagga cggcgtactg gtcgtgctcg
      901 ccacgatggg agggagccgg gtcgattcgc tggacctcag gacgctcttt cggcgccggc
      961 tctcggtcgt gacctcgacc ctcaggaccc ggtcggccga ataccgttcg gagctgacgg
     1021 ccggcttcgt gcgcgacgtg attccgctgc tcgcctcggg caaggtcgcg cccgtgatcg
     1081 acacggtgct gccgtggacc gatgtcgccc gggcccaccg gcacatggag gccaatcgga
     1141 acgtgggcaa gatcgtgctc ctggtcgact gaccgggcgg gccggcgctc agacgggctc
     1201 cgcgccgatg cccgggcggt ccgtgggcag cacgcgtcct tcccggattt ccacgccatc
     1261 gaacggatcg tcccggacga gcaggggagc gtccaggtcg gcgaagtcca ccagcggagc
     1321 gagatgggcg gcggccgcga cggcgaggct gctctcgatc atgcagccca ggagcacctg
     1381 caggtccagc gcgcgggcca tggcgatcag gacacgcgcc ggcgcgatgc ccccggcctt
     1441 ggcgagcttc acgttgatgc cgtcggccgc tccggccagg gcaatcacgt cgtccacggt
     1501 gaacacgctc tcgtcggcaa agagggccgg tacccgggtc gaaccgagcg cccgcctgag
     1561 cctgtaccag tcgtcgggat cgccggccgc gatgggctgc tccacgatcg agactccggc
     1621 ctcgagcagc gccgggagca tgtccaccgc ctcgtcccgg gtccaggcgc cgttggcgtc
     1681 gacgacgatc agcgcatcgg tcgccccccg cgccgtccac acgacctcca ggtcccgttc
     1741 cggatttccg gtgccgacct tgagcttcag gcggtcgtgt ggcccgaacg accgggcgtc
     1801 gcgggccaga tcgtcgaggt cctcgggaat ggaaagggtg acgaagctga ccgggctgca
     1861 gcgcggatcg aggccgaaca gcgtgtacag cggatacccc agccgacgcc cccagaggtc
     1921 gtgaagggcc atgtccaggg cgcaccgggc ggccggcgga ccgggcggaa gcccctcgag
     1981 gacgtgctcg agcggtatgg ggccctcgtc gggaagatcc ggaagcgaaa cgcccttcat
     2041 ccactccgcg acgtcgtcca ccgtggtgcc caggtagggc gggaggccgg cctcgccgaa
     2101 ggcctcgccg aggcggacca ggacgttcgt tcgcaccgtg ctggtcccgt gcgcgatgcg
     2161 gaagggacgg accaggtgga cgggcagcgc ggagacttcc ggcgtcatgt cggtgccccg
     2221 ttgccggagg gcccggcgtg ggagaggcaa agcggggcca cggctccgcc gcaggcgagg
     2281 gacgcgaggt gcgcggccgc ctgcccggga gacgtgcaca gcggggcgtc gccgaagggt
     2341 cgtaccccgg cggccgggaa cccctcgacc ctgagcccgt cgaccgccgc gggaggcgat
     2401 ccgaaccggg cgcgccagga gcgcagagcg gtccagtccg cggatgacgc ccgcgccgag
     2461 acgaggatca cggcacggac gtcggctgcg cccgcgagtg cggatcgaat gtcggcgagg
     2521 cccggcgacg tccgaaccgc cgccgccggc tcctcggcct gcacggccgc ccaggggagg
     2581 aggacctcga tgcgcggccg acccgtgcgg ccgagcggca ggcgcatgca cgcggcgaac
     2641 aggcgctcgg caagggcggg gggagcgtcg gagcggacac gcagcaggcg atccaggagg
     2701 tgccggtcca cgcccagccc gggccgcgcg cacgtgaccg cgaggcgaac ccggctgtcg
     2761 cgctccagaa ggccgggaat ccagtcgagg ggcatggtcg acgagccggg gcccacggcc
     2821 aggcagatcc ggccggccgc tccggattcc gcgcgctccg cctgtgcgcg gagcgaatcg
     2881 aggacggact cggccaccgg tcggggctcg cgaaccggca ggtcgacgga ggtccggatc
     2941 cagaggtcga gccgggtctc cccggaaagc cgctcacgga ccgccgacag gccgcccgtg
     3001 gagtccggtg ccgccggctg gtcgaccagg atcacggggg ccagggaggc gggaaaagat
     3061 ccgggcccga tctcgccgag gaccagggcg gcggtccgcc ggacctcctc cggggaggct
     3121 ccggggccga gctcgtcgat cacgcggtcg acgaggcttc tgatcagcgc ggggtcgcca
     3181 gtgccgggct tcttcatggt cctaacctag gaatgcccgg attcagacga aagggaatcg
     3241 gggccccacg cgcgagcgtg gagccccgat tgcggtcccg cgatgcaccc gcggctacgg
     3301 ccgggtgagg gccgtgaaaa aggtgctgac ctggccctgc tgcgggcgga ggaccttcac
     3361 gatgaaactg tccccggact tcacgtcggc gatcaccgag cggagttcct cgggcgacgt
     3421 gacgggcctg cggtccgcct cgacgatcac gtccccctgc cgcacgtcgg cttcccggta
     3481 ggccgtgctc tccgtgtcga tggccgagac caggatgccg gtggtgcccg ccggaaggcc
     3541 gaggcgctcg gccgcggtcg cggacagcgg cctcagggtc atcccgaggg caccgagctc
     3601 gtcggtctcc tgcgtgccgc ccggaggagc ggcctcggcc gttgcgtcca cgaactcgga
     3661 ccgccggccg agttcgacgc ggacgaccct gctctcaccg tcccggttca gcgtgagctc
     3721 cacccggtcg ccgggagcca tgttgccgac ccgggtcctg agctcgtcgg cggatcgcag
     3781 ctcgtgaccg tcgacggccg tgatgatgtc ccctgccttc agccccgcgt cctcggcggg
     3841 cgatccatcc gtgacgctcg tgatctgggc cgaaccacgc ggcacgccca tcgcctcggc
     3901 gagcgactgg ctcacgccgt cgaagaggac gcccaggaat ccacgttcaa ccgttccgga
     3961 gtcgatgagc tggtcggcga cgttcttcac cacgtccacc ggaatcgcga agccgatgcc
     4021 ctggtagccg cccgaccgcg agtagatggc cgaattgatg ccgatgagat ggccccggag
     4081 atcgacgagc ggtccgcccg agttgcccgg attgatggcc gcgtcggtct ggatgaagga
     4141 cgcgaagagg ttcagtgcag tgaggttccg cgacgtgcgg ccgaccgcgc tcacgatgcc
     4201 ggaggtgacc gtgttgccga gctcctcggc gagcggcgag ccgaatgcca gcacccactg
     4261 gcccggtcgc acgtcctcga tcgacccgaa cggaacgacc ggaagcccct gcttctcgat
     4321 ccggatcacg gccaggtcgc tggcctcgtc cgagccgacc accgtcgcct tgtggaaggt
     4381 gccgtcggac agcacgacct cgtggtcgga cgcctcgcgg atcacgtggt agttcgtgac
     4441 gacgtacccg ttctcgcgga cgatcacgcc cgagcccagg gacctcgtcc ggaaggtgcg
     4501 gggctgctgg tccggcatgc ggaaccagtc ctcgaacgga gaaccctcga acggattgat
     4561 ggactgctgc tcccggacct gctcggagcg gatctggacg acggccggat tcacgatctc
     4621 ggccacgtcg acgaacgccg tctcgaaggc gagcgcgcgc ggatcgagag cgggacgccg
     4681 cgattccgaa gtggcctggg aggcgtacga ggccgttccg atccggtcgc cggccccgag
     4741 ccagttgccg ccgacggtgg cggaaaggat gccggctgcg aacgttgcga gcaggagcag
     4801 ggagagcgag aacctgcctt ttgcgctcat ggtcatgacg atctgtatga tgatgctgac
     4861 tgttccggga gccggtccgc caggatggca gggcccggca tggcctttct gatggcgaaa
     4921 aacgcgtgcc ggaatcgatg ggtccggcag tgccccggta atctggaacg atcgtgaagg
     4981 ccgatttcgt atgtccggcg catgcccgat ccgggagcgg gggcgggatg ccgccgtcag
     5041 gcgggcgcgc gttcggcgat cgcggcgaga agccgcgcgt ggatgttgcc gaacccgccg
     5101 ttggacatga cgaggaccac gtcccccgga cggatccagt cgatcagggc gggcaggagc
     5161 tcgtccgcgt cccggccgag cgccgtgtgg atgccgcgcc gctcgagcga ggccgtgacg
     5221 cgcccgatgt ccatgaaatc ctcggcccgg tcgttatgac ggaacggggg cgtgctcagg
     5281 aatacggcat ccgcgcggtc gaatgcgcgc atgtagccct cctcgaagac cttgcggcgg
     5341 ctggagttcg aacggggctc gaagacggcg acgaggcgcc gctccggcca gcgctcgcgc
     5401 acggcgtcga tcgtcgcgcg cacggccgtc ggatggtggg cgaagtcgtc gagcacggtc
     5461 acgcggtcga cctcgccccg gatctcctgc cggcgccgta tgccctcgaa cgtggcgagc
     5521 ccttcacgga gtgcaccggg cgtgacgccc tccccgaggg ccacggcgca gaccgcgaga
     5581 gcgttcgaga gattgtgccg gcccgacatc ggcaggaaga acgtgcccag ggccgttccc
     5641 cggtggacga gcgtgaatcg ctggccgtct ccctcgctcg agatgccgcc cgcggtgacg
     5701 tcgaggtccg ccgcggcgag cccgtaggtg accacccgtg cacccgtgtg ccgtgccagc
     5761 gccctgacct ccggatcatc ggcgttcagc acgagggacc cggccgggtt caccgtgcgg
     5821 gcgaattccc ggaacgcgtc gcggtaatcg ctccacgtgt cgtagatgtc cgcgtggtcg
     5881 aattccaggg acgtcacgac cgcgtgggtc ggccggtaat ggagaaactt gggacgcttg
     5941 tcgaaatagg cgctgtcgta ctcgtcgccc tcgatcacga agtgggagcc cgcaccgacc
     6001 ccgtagctca cgtcgccgtt gcgcatgacc ccgcccacca ggaacccggg atcgagtccg
     6061 gcggtccgca gcacgtggac gaggaggctc gtcgtggtcg tcttcccgtg cgttccggcg
     6121 acgacgagcg agacccggtc gcggatgaag tagtgcgcta tggcctcagg aagggaagcc
     6181 tgcgccagcg aggactcccg ggccctcgcg gcctcgacat gcgtcggcgt gcacgcgtta
     6241 ccgacgacga cgagccccgg acggggatcc aggtggccgg catcgaatcc ctccaggacg
     6301 gggatgccga gctccccgag ccgggtggac atggggggcc acgcggcggc gtcggatccg
     6361 cgcacgcggt acccggcctg gtggaacagc cccgccaggg atcccatccc ggtcccgcag
     6421 atgccgatca ggtagacgtc ctcgaaggcc tccgcgtcgg ggagtgccgg ccggtcgaag
     6481 acacggagat gggcgtcggg caggtcctgg atgcgcctca tcgtttcggg gccgcgaccg
     6541 gacggcgaac ctcctcgacg tcggtggcgg acacgtcgcc ggccagcccg tcgccgtcga
     6601 agcctcccgc ggggcgcatc ggcccgtgca cgtgctcccg gaagtacccg gtcacgcgct
     6661 ccgacaacg
//
LOCUS       DBNHQZ010000379         3047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2821467, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000379 DBNHQZ010000000
VERSION     DBNHQZ010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2821467"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="AC16YU_10265"
     CDS             <1..1290
                     /locus_tag="AC16YU_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065653.1"
                     /translation="WIYLADLQGISLSRIGPNGEREDLSQGVRCNCGLPADGRDGDRV
                     VVSGRDREVAVAFRADGARDTLAIRGNNVTFLPRGQVVYTRTGRLVASLWNPVTGEVG
                     ADSRVMLDDVRTGSIIRSAHYGISDAGTLVYVAGEPSGLVDLVIRESDGTDTRLPLPY
                     GVYGPVNVSPDESRLLVKDYDRGAQTVMVDMERGGQQVVLPDARDVSVMWSPDGRRIT
                     YVRPSAGDDSEIWEADPNRPSDAELVVRETGNVRVSAWSLDGAWLAYDRQNGAGRELV
                     VRRMRDSTDFVIRNAAGQTLWALDFSRDSRILAYTRVAQGGSAILVDTVPPGGDPVVI
                     AAGGAEEPEWLPDRDALVYRQGDTWYIVDVLDAGNLSFSVPRILFRGPYVNIAGMEYR
                     MMSGGRAVLQLPRNTAATADRIEVITGFDRLIEQAFE"
     gene            1287..>3047
                     /locus_tag="AC16YU_10270"
     CDS             1287..>3047
                     /locus_tag="AC16YU_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="HIR6065654.1"
                     /translation="MIGFSFLQYRIDAELGRGGMGIVYRATDTNLDRTVALKVLPPHA
                     LASEDDRARFYREARAAAKLSHAHICHVYQVDEAVLVEEGASRAPDAEKRPFIAMEYV
                     DGESLAERIAKGPLKLEEAIRIASEVASALGAAHEAGVVHRDVKSGNVMLTKKGEAKV
                     LDFGLAKTASSTQLTRQGSTLGTVAYMSPEQARGEEVDARTDLWALGVVLYEMIAGRL
                     PFAADYEQAALYGILNEDPEPLTALRTGVPMELEAVVAKLLRKESRLRYRAASDVIAD
                     LEAISLGSRAGLSARSVAALPARAAERMVPEGRARLYAAWAGLACLLVGALAAAAVLG
                     RGDDRETVPVSTKRLTQAVPIRGSAVTLDLSPDGRKVAYVSDIVRVLDLESGRITEIP
                     EAGGVITVDFSPDSGSLLLTRSSDVLRIPASGGSPIVVVTSREGGPRAVWGPGDLVFY
                     EEDNRIFKASLAGGVPSPVTTLDEEAGEFDNDWPEVLPGGRVLMATIEILDQPARIGF
                     WDLESGERLGTLDTPGYGAQWVPTGHLLMVMDGSAVAVPFDPASLRQTGPVIPVLPAA
                     ARDGMSFAGDGTFVYTGADIG"
BASE COUNT          468 a          957 c         1133 g          489 t
ORIGIN      
        1 tggatttacc tggcggatct gcagggcata tccctttcgc ggatcgggcc gaacggggaa
       61 cgggaggatc tctcgcaggg cgtgcgctgt aattgcggac ttccggcgga cggtcgggac
      121 ggggaccgcg tggtggtatc gggccgggac cgcgaagtgg ccgtcgcgtt cagggcggac
      181 ggcgccaggg acacgctggc cattcgcggc aacaacgtga cgttcttgcc gcggggacag
      241 gtcgtgtata cgcggacggg gcggctggtg gcctccctct ggaacccggt aaccggtgag
      301 gtgggtgctg acagccgcgt gatgctggac gacgtcagga ccggttccat catccgttcc
      361 gcccactacg gcatttcgga tgcgggcacc ctcgtctacg tcgcggggga gccgtcgggg
      421 ctcgtggacc tggtcatccg ggagtccgac ggcacagata cccggctgcc cctgccctac
      481 ggcgtatacg gaccggtgaa cgtgtcacct gacgagtccc gactgctcgt caaggattac
      541 gaccgtggtg cgcagaccgt catggtagac atggagcggg ggggtcagca ggtggtgctg
      601 ccggatgcgc gcgacgtctc cgtcatgtgg agtccggacg gacgacgcat cacgtacgtg
      661 cggccgtccg cgggagacga ttccgaaatc tgggaggccg acccgaaccg gccttccgac
      721 gccgagctcg tggttcggga aaccggcaat gtcagggtgt ccgcctggtc gctggacggc
      781 gcgtggctgg cgtacgatcg tcagaacgga gccgggcgcg aactggtcgt tcgccgaatg
      841 cgggacagca ccgatttcgt gatccgcaac gccgcgggcc agaccctctg ggcactggac
      901 ttttcgcgag acagtcgaat actcgcctat acgcgggtcg cgcagggagg ctcggccatc
      961 ctggtcgaca cggtgccgcc cggcggcgac cccgtggtca tcgcagcggg aggcgccgag
     1021 gaaccggaat ggcttcccga ccgcgatgcc ctggtctacc ggcaggggga cacctggtac
     1081 atcgtggacg tgctggacgc cgggaatctc tccttctccg taccccggat cctgttcagg
     1141 ggtccttacg tgaacatcgc cggcatggag taccgcatga tgtccggcgg gcgcgccgtc
     1201 ctccagctgc cgagaaacac ggcggcaacg gcggaccgca tcgaggtcat caccggattc
     1261 gaccgactga tcgaacaggc attcgaatga tcggcttcag ctttctgcag taccggatcg
     1321 acgccgagct cggacgtgga ggcatgggca tcgtgtaccg ggcgacggac acgaatctgg
     1381 accggacggt cgccctcaag gtccttccgc cgcatgcgct cgccagcgag gacgaccggg
     1441 cccggttcta ccgggaggcg agagcggcgg cgaagttgag ccacgcccat atctgccacg
     1501 tgtaccaggt ggacgaggcc gtgctggtcg aggagggggc ctcccgtgcc ccggacgccg
     1561 agaagcgccc cttcatcgcg atggagtacg tggacggcga gtcgctggcc gagcgcatcg
     1621 cgaaagggcc gctgaagctc gaggaggcga tccggatcgc gtccgaggtg gccagcgcgc
     1681 tcggcgcggc gcacgaggcg ggtgtcgtgc atcgcgacgt gaagagcggc aacgtgatgc
     1741 tgacgaaaaa gggcgaagcg aaagtgctgg acttcggcct ggcgaagacg gcgtcgtcga
     1801 cgcagctcac gcggcagggc tcgacgctgg ggacggtggc atacatgagc ccggagcagg
     1861 cgagggggga ggaggtcgat gcgcggacgg atctatgggc gctgggcgtc gtgctgtacg
     1921 agatgattgc cggtcggctg cccttcgccg ccgactacga acaggcggcg ctctacggca
     1981 tcctgaacga ggaccccgag ccgctgactg cccttcggac gggcgtgccg atggagctgg
     2041 aagccgtcgt ggccaagctc cttcgcaagg aatcgcgcct gcgataccgg gcggcgtcgg
     2101 acgtgatcgc ggacctggag gccatctcgc tcggctcccg agccggtctg agcgcgcgaa
     2161 gcgtcgccgc cttgccggct cgggccgcgg aacgcatggt gcccgagggg cgggcccgtc
     2221 tctacgcggc gtgggcgggc ctggcctgcc tgctggtcgg agccctggcg gccgcggcgg
     2281 tgcttggccg gggcgacgac agggagacgg tcccggtctc cacgaagcgg ctcacgcagg
     2341 ccgtccccat ccggggctcg gccgtgacgc tggacctgtc gcctgatggc cggaaggtcg
     2401 cgtacgtttc ggacatcgtc cgcgtgctcg acctggaatc gggccggatc acggagattc
     2461 cggaggccgg cggcgtcatc acggtcgatt tttcgccgga ctcgggctcg ctgctgctca
     2521 cgagatcctc ggacgtcctg cgcattcccg cgtcgggggg ttcgccgatc gtggtcgtga
     2581 cgtcccggga gggtggcccc cgggcggtgt ggggccctgg cgatctcgtc ttctacgagg
     2641 aggacaaccg gatcttcaag gcatcgctgg ccggcggcgt cccgtctccg gtcaccacgc
     2701 tcgacgagga agcgggggaa ttcgacaacg actggcccga ggtgcttccc ggaggccgcg
     2761 tgctcatggc gacgatcgag attctcgacc agccggcacg gatagggttc tgggacctcg
     2821 agtccggcga gcgcctcggg acgctggaca cgcccggcta cggcgcgcag tgggttccga
     2881 cgggacacct cctgatggtc atggacggtt cggcggtcgc cgtgccgttc gatcccgcct
     2941 ccctgcgcca gaccggtccc gtgatcccgg tgctgccggc tgcggcgcgg gacgggatga
     3001 gcttcgcggg cgacggtacg ttcgtgtaca ccggggctga catcggg
//
LOCUS       DBNHQZ010000380         5717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_385065, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000380 DBNHQZ010000000
VERSION     DBNHQZ010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_385065"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="AC16YU_10275"
     CDS             complement(<1..1320)
                     /locus_tag="AC16YU_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6065655.1"
                     /translation="MPQSTLIHGILVLAALVTFAWVIRKRAVLLDRAAPDPRLDHVGQ
                     RLARLLVVGFGQSRQPRYLVAGVLHILIFAGFLVLSVRSLTLLGEGFSPGFALPGLRG
                     DAGHAYAVVKDFTALVVLLACVVAVVRRFAFHPARYHDRTARSAHGGEAYVILGLISL
                     LMVADALYEGTAPGATASAGMPLAGLAAAALGGVEAAALATIHYASFWIHNVALLFFL
                     CYLPVSKHFHVVTALPNVFLSKLRPTGHTKPPRHDEPDLGRIEQVGVGFLEDFTWKHV
                     LDFYSCTDCGRCSDHCPAYTTGTPLSPRMISIKSRDCAYSRYPVFGPAPAAGERTPLV
                     GDVITAPELWACTMCGACEEACPVTIEYVDKIVDMRRFLVDQGEVPEALQKSLSDLEQ
                     KGNPYGKPARQRADWLSGSEGVRVLGPGEEADLLYFADSTTSFDPRIQ"
     gene            complement(1660..2424)
                     /locus_tag="AC16YU_10280"
     CDS             complement(1660..2424)
                     /locus_tag="AC16YU_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065656.1"
                     /translation="MNRVVGAIALLAAASVGFGAPAAAAQSTSGTFRVHAPGVPAATV
                     DAVQGDLAFAEERITRMLGPFPDSVSVWILANREGFSEALRDAWGLEDTACWMVGAAS
                     DGVLLLLSPAVWGDEACEHDPADEMHRRLLVTHEAVHAFHGLTNPSEDIGPLEDLGWF
                     TEGLATYVSGQLVDSHAGRAREAIDGGHDPERLDDAWSGPYRYGVTGSMVAFIDEHWG
                     REVLRQALSAESKAAVLALLRTTEAGFLQEWRAWVP"
     gene            2628..3101
                     /locus_tag="AC16YU_10285"
     CDS             2628..3101
                     /locus_tag="AC16YU_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065657.1"
                     /translation="MSISRLHCLLAFVAVSLTMLGCAPSGEQPADTPAEPEVVEVMNP
                     LAPFAGTWSATTYAPDSDEPTMTFTMTATDSTGGWSMHFGHLDAPVPARVVGMMGDSL
                     TLEFGNYASGLREGAMVDRVRSTLIVVGDSLAGRFEATYVDGGTRSGRLAGVRAQ"
     gene            complement(3174..4415)
                     /locus_tag="AC16YU_10290"
     CDS             complement(3174..4415)
                     /locus_tag="AC16YU_10290"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6065658.1"
                     /translation="MEAPTDRHDQIQDFLLLQRVAQRIGSILDLDELLEQIVDDVAQT
                     FGYLRSGILFLDAVRGDIVIAAVRGWTSHYHVKGDRFTMDEGMIGHVARTGQPYYAPD
                     VTKDPYYMVSETSTRSEVDIPIRSRGRIIGVFNAQHAEVDAFPAHRLQLLEALAGHIG
                     VAVENARLFERERHEKERMVRDLAEARAIQLRLFPAHAPEMRNLRVTGLSMPCLAVGG
                     DWYDYIPLPGGRMAIVLGDVAGKGPGAALLMTSTRSIVRMHAELGAGPASVLSQVNDV
                     LVADLPASKFVTMIYAVVDPDRRSVTFASAGHLPPVAAGGSGVRVVRTQPDLPVGIRE
                     WSYSEHTAELHPGDRLFLYTDGVTEARSRASEEYGEARLLRHVGNPGASAESLLQDVQ
                     THSEGHPSVDDITIVVVEALK"
     gene            complement(4571..5605)
                     /locus_tag="AC16YU_10295"
     CDS             complement(4571..5605)
                     /locus_tag="AC16YU_10295"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR6065659.1"
                     /translation="MTLPAACRRLLPSLLVLTALLGACRAQAQQPPDPFAQNERLGRG
                     VNIIGYDPIWHERDEGRFTDAHFRLIREGGFSSVRINLHPFRHMGPADEYRLEESWLD
                     VLDWAVEEALEQDLMVILDLHEFNAMADDPEGKKDMFLAFWRQIAPRFKDAPTTVLFE
                     VLNEPSRGVSPEMWNGYLREALAVIRETNPHRTLIIGPARWNGIGALEELDLPDEDRG
                     VIVTVHYYSPMEFTHQGAPWTEAYRDTLGVRWLGTPDEMERIRSDFELAQQWSLAHDR
                     PIFLGEFGAYDKGDMDSRARYTAAVARTAEELGWSWAYWQFDSDFILYDIDNDRWVEP
                     IHDALVPPGE"
BASE COUNT          946 a         1904 c         1964 g          903 t
ORIGIN      
        1 ctggatcctc ggatcgaacg aggtcgtcga gtcggcgaag tacaggaggt cggcttcctc
       61 tccggggccg agcacgcgca cgccctccga gcccgagagc cagtccgccc gctgtcgggc
      121 cggcttgccg tagggattgc ccttctgctc caggtcggag agcgacttct ggagcgcctc
      181 gggcacctcg ccctggtcca cgaggaagcg ccgcatgtcg acgatcttgt cgacgtactc
      241 gatcgtgacg gggcacgcct cctcgcatgc tccgcacatc gtgcacgccc agagctccgg
      301 cgcggtgatc acgtccccga cgagcggcgt gcgctccccg gcggcgggag ccgggccgaa
      361 cacggggtac cgggagtatg cgcaatcgcg gctctttatg ctgatcatgc gcggcgaaag
      421 gggagtgccc gtcgtgtacg cggggcagtg atcagagcac cgtccgcagt ccgtgcacga
      481 gtagaagtcg aggacgtgct tccaggtaaa gtcttcgagg aagcccacgc cgacctgctc
      541 gatccttccc aggtcaggct cgtcgtgccg cggcggtttg gtatggccgg tcggccggag
      601 cttcgacagg aacacgttcg gaagtgccgt caccacgtgg aaatgcttgg aaaccggcag
      661 atagcacagg aagaagagga gcgccacgtt atggatccag aagctcgcgt agtggatcgt
      721 ggcaagcgcc gcggcctcca cgccgccgag cgcggccgcc gcgaggccgg cgagcggcat
      781 gccggcgctt gccgtggctc ctggggccgt tccctcgtac agggcgtccg ccaccatgag
      841 cagcgagatc agccccagga tcacgtacgc ctcgccgccg tgcgcggagc gggccgtccg
      901 gtcgtggtac cgggccgggt ggaacgcgaa gcgccggacc acggccacga cgcaggcgag
      961 gagaaccacc agcgccgtga aatccttgac gacggcgtac gcatggccgg cgtcgccccg
     1021 aaggcccggc agggcgaatc cgggagagaa tccctctccc agcagggtca gcgaccggac
     1081 ggacaggacc aggaaaccgg cgaagatgag gatgtgcagc accccggcga cgagataccg
     1141 gggctgtcgg gactggccga agccgacgac cagcaatcgt gcgagacgct gccccacgtg
     1201 atcgaggcgc gggtccggcg ccgcgcggtc gagcagcacc gcgcgcttcc ggatcaccca
     1261 ggcgaaggtg accagcgcgg cgaggacgag aatgccgtgg atgagcgtcg actggggcat
     1321 gggcaagcgc gaatggcgaa atttcgctcg tggcctgcgc ccatcctaat cagttcccgg
     1381 ccgtctggca agggggattt ccccacatcc cgccgcgggg atggccagat cgcccggtgc
     1441 cgtccggcga tggtgcgcgg cccatccggg gctcgggtac ggcatgcttc ggttccggcc
     1501 cctgtggaag tgagtgcgag atggtttgcg gcgcaaacga agttgcacgt cttccatacg
     1561 tcgccgggat tcaacccatg agatgcgcga ccagcgcaac gcgcgcgcct acggcggctt
     1621 gaagaccggc cctgcggggc cagagaggga accggtccgc tacggtaccc aggccctcca
     1681 ctcctggaga aaccccgcct cggtcgtccg aaggagtgcg agcaccgcag ccttcgactc
     1741 ggcggagagc gcctgccgaa gcacctcgcg cccccaatgc tcgtcgatga acgcgaccat
     1801 cgagccggtc acgccgtagc gatacggccc ggaccaggca tcatccaggc gctcaggatc
     1861 gtggcctcca tcgatggcct cccgcgcacg gcccgcgtgc gaatccacga gctgacccga
     1921 cacgtacgtc gccaggccct cggtgaacca ccccaggtcc tccagaggcc cgatgtcctc
     1981 gctcgggttg gtcaggccgt ggaaagcatg caccgcttcg tgggtcacga gaaggcggcg
     2041 gtgcatctcg tccgcgggat catgctcgca cgcctcatcg ccccacacgg ccggggacag
     2101 gaggaggagc acaccgtcgc tcgccgctcc gaccatccag cacgcggtat cctccaggcc
     2161 ccaggcatcc cgcagcgcct cggaaaatcc ctcgcggttc gccaggatcc acaccgaaac
     2221 ggaatcgggg aaggggccga gcatcctggt gatccgttcc tcggcgaagg cgagatcgcc
     2281 ctggacggca tcgacggtgg cggccggcac gcccggcgcg tggacgcgga aggtcccgga
     2341 ggtggactgg gcggcagcgg ccggcgcgcc gaaaccaaca gaggcggccg ccagcagcgc
     2401 aattgcgcca acgacacggt tcatgggacc ggtacccgct ctcgcgagct tctgcggaga
     2461 tgcggaccgt gcttacgggc acctcgacgc ggagttgccg gccgggggtt tcggtatccc
     2521 ggctccgaaa ttctcagccc cgcactcctg cccccggcag ggtcacttgc gagtgcactg
     2581 cggattaccc taccatgggc acccagttta atctccggat gacaaccatg tccatctccc
     2641 gtctgcattg cctccttgcc ttcgtggcgg tctctcttac gatgctcggg tgtgccccca
     2701 gcggagagca gcctgccgat accccggcgg agcccgaggt cgtcgaggtg atgaatcccc
     2761 tcgccccgtt cgccggaacc tggagcgcca cgacctacgc tcccgactcg gacgagccca
     2821 ccatgacgtt cacgatgacg gctaccgact ccaccggcgg gtggtccatg catttcggcc
     2881 acctggatgc accggtgccg gccagggtgg tgggaatgat gggtgactcg ctgacgctgg
     2941 agttcgggaa ttacgccagt ggccttcgcg agggagcgat ggtcgaccgg gtgaggtcca
     3001 ccttgatcgt tgtgggtgac tcgctcgccg gtcgcttcga ggccacctac gtggacggcg
     3061 ggaccagatc cggtcgcctg gcgggcgtgc gcgcgcagta gaagcttcgc cggtcggccc
     3121 acggggtcgt cgcagacccg agccgatccg ccggatcacc cgtctgcaag cggctatttc
     3181 agcgcttcga ccacgacgat cgtgatgtcg tcgacggagg gatggccctc ggaatgcgtc
     3241 tggacgtctt gcaggaggct ctcggccgac gcacccggat tgccgacgtg tctgagcagg
     3301 cgagcctcgc catattcctc ggacgcacga ctccgcgcct cggttacgcc gtcggtatac
     3361 aggaagaggc gatcacccgg gtgcagctcc gccgtgtgtt cggaatagct ccactccctg
     3421 attccgacgg gcaggtcggg ctgggtcctg actacgcgga cgcccgatcc gcccgcggcg
     3481 acgggaggga ggtgtccggc actggcgaaa gtgacggacc ggcggtccgg gtcgacgacg
     3541 gcgtagatca tggtgacgaa cttcgacgcc ggcaggtcgg ccacgagcac gtcgttgacc
     3601 tgcgagagca ccgaggccgg tccggcgccc agctccgcat gcatccggac gatgctccgg
     3661 gtcgacgtca tcaggagcgc ggccccgggt ccctttccgg cgacgtctcc gagcacgatc
     3721 gccatccggc cgccgggaag ggggatgtag tcgtaccagt cccctccgac ggcaaggcag
     3781 ggcatcgaca ggccggtgac gcgcaggttt ctcatttccg gggcgtgggc cgggaagagt
     3841 ctcaactgga tggcgcgcgc ttccgcaagg tcccggacca tgcgctcctt ctcgtgccgc
     3901 tcccgctcga atagccgtgc gttctcgacc gccacgccga tatgcccggc aagcgcctcg
     3961 aggagctgga gccggtgggc ggggaaggcg tccacttcgg cgtgctgggc attgaacacg
     4021 ccgatgatcc gtcccctgga tcggatagga atgtcgacct ccgaccgcgt ggaggtctcg
     4081 ctcaccatgt agtacgggtc cttcgtgaca tcgggcgcat agtacggctg ccccgtccgc
     4141 gccacgtggc cgatcatgcc ttcgtccatc gtgaagcggt cccccttcac gtggtagtgc
     4201 gatgtccagc cccggacggc ggcgatgacg atgtcgccgc gcaccgcatc caggaagagg
     4261 atgccggagc gcaggtagcc gaacgtctgc gccacgtcat ccacgatctg ctccagcagc
     4321 tcgtcgaggt ccaggatcga tccgatgcgc tgggcgaccc gctgaagcag caggaagtcc
     4381 tggatttggt cgtggcgatc ggtgggtgct tccatgggca ggggcgccca gatcctgggt
     4441 ggcctgatcg tcggaaggcg cgtcgtcccc gggcagtcgt acgtgccgtc gcgcatgcta
     4501 cgtcgggagc acgaaaaggg tacgcgcggg gctccgtcga ggccggcgaa ttcaagcgcg
     4561 tccgcccgcc ctattcgccg ggcggaacga gcgcgtcgtg tatgggctcg acccagcggt
     4621 cgttgtcgat gtcgtacagg atgaaatccg agtcgaactg ccagtaggcc cagctccagc
     4681 ccagttcttc cgccgtccgc gcgacggccg ccgtgtaccg ggcccgcgaa tccatgtcgc
     4741 ccttgtcgta ggccccgaac tcgccgagga agatcggccg gtcgtgggcg agcgaccact
     4801 gctgcgccag ctcgaagtcg ctgcggatgc gctccatctc gtccggggtg ccgagccacc
     4861 ggacgccgag cgtgtcccgg tatgcttccg tccagggagc tccctggtgt gtgaattcca
     4921 tcggcgagta gtagtgcacc gtcacgatga cgccccggtc ttcgtccggc aggtccagct
     4981 cctccagggc gccgattccg ttccagcggg cggggccgat gatgagcgtt cgatgcggat
     5041 tggtctcgcg gatgacggcc agcgcttcgc gcaggtaccc gttccacatt tccggggaaa
     5101 cgccgcgcga cggttcgttg agcacctcga agagcacggt ggtcggagca tccttgaacc
     5161 gcggggcgat ctgccgccag aaggcgagga acatgtcctt cttgccctct ggatcatcgg
     5221 ccatagcgtt gaactcgtgc aggtccagga tcaccatgag atcctgctcc agcgcctcct
     5281 cgacggccca gtccaggaca tccagccagc tctcctcgag ccggtactcg tccgcgggcc
     5341 ccatgtggcg gaacgggtgg aggttgatcc ggacggaact gaagccccct tcacggatga
     5401 gtcggaaatg ggcgtccgtg aagcgtccct cgtcgcgttc gtgccagatc gggtcatacc
     5461 cgatgatgtt cacgccgcgc cccagcctct cgttctgagc gaacgggtcg ggtggctgct
     5521 gcgcctgagc ccgacaggcg ccgaggagag cggtgagtac gaggagggag gggaggaggc
     5581 gcctgcacgc agccgggagg gtcatgtcac gcatcttggt gggtggacgg aggccgcggc
     5641 gcggccggct gcgggcccac cgcggaccgg gcacacggtt cggaggagtg ctccgaacag
     5701 taacggacag acggtcc
//
LOCUS       DBNHQZ010000381         4309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_113179, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000381 DBNHQZ010000000
VERSION     DBNHQZ010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_113179"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1290)
                     /gene="nadA"
                     /locus_tag="AC16YU_10300"
     CDS             complement(298..1290)
                     /gene="nadA"
                     /locus_tag="AC16YU_10300"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6065660.1"
                     /translation="MQLPVILDPAIGYVDEPVDPGLDLYEEIERLKREKNAVLLAHYY
                     QEPAIQDIADFIGDSLGLSRKAAATDADMIVFAGVHFMAEVAKILSPRKKVVLPDLNA
                     GCSLADSCPPDLFAAFKERYPDHIVISYINCSAATKALSDIICTSSNAAHIIRQIPED
                     QPIIFAPDRNLGRYLVKETGRDMVLWDGVCVVHETFSEERLVRLKVRFPDAPVLAHPE
                     CEEAVLRQADFVGSTSQIRRHASENPASTFIVATEEGILHQMRKDNPDKTFVAAPPES
                     GCACNQCPHMRLNTIEKLYLCMKHEAPEITMDEQLRLRALAPIEKMMAMSEGVK"
     gene            complement(1406..2203)
                     /locus_tag="AC16YU_10305"
     CDS             complement(1406..2203)
                     /locus_tag="AC16YU_10305"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6065661.1"
                     /translation="MTLPFLPAGVIGCVVAAVLAYTALVWLLSLALRDSSIMDVAWGP
                     GFLVAAGAAAVSVGASGGRVAWVLALTATWGFRLGGHILAVAIGRGAEDARYAKWRRE
                     AGRGWWWRSFFKVFLLQGVILCVVALPLVVQVAARGPGVPTEWDVAGIAIWLAGFAIE
                     SVADWQMLRFRRTRTAKGSVMDRGLWRWSRHPNYFGETLVWWGFGLMALSVPGGWVAL
                     AGPLVITWLIVKVSGVAMLERALAETKPGYADYARRTSAFIPWKPRD"
     gene            2283..2723
                     /locus_tag="AC16YU_10310"
     CDS             2283..2723
                     /locus_tag="AC16YU_10310"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR6065662.1"
                     /translation="MIRPSVLFVCTHNSARSQLAEALLRARFGDRYQVYSAGTVKTRV
                     KPEVLTVLAEIGIDASAHRSKSVDDLGGRSFDYVVTVCDSAKEACPFVPAVRARTHAP
                     FEDPSDVTEPPGARLDAFRDARERLAGWIDRVFAGPEPDFDAGG"
     gene            complement(2739..4178)
                     /locus_tag="AC16YU_10315"
     CDS             complement(2739..4178)
                     /locus_tag="AC16YU_10315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR6065663.1"
                     /translation="MSDLLEKIVSLSKRRGFIFQSSEVYGGLGATYDYGPLGVELKRN
                     VAQRWWDAMVYRNENVAGLDAAILMHPTVWKASGHVDAFNDPMIDDRVSKIRYRADQL
                     IEGHVDRLRRKGKDADADRVYDALVDALNAEDPAKALYDVILREEIKSPDSGMADWTE
                     VRQFNLMFETRLGALAGETNTIYLRPETAQGIFVNFHNVREASRLQVPFGIAQIGKAF
                     RNEIVARQFIFRMREFEQMEMQYFVKPGDQMEAFEAWRERRMQWHVDNGVGASRLRWH
                     EHEKLAHYADAAFDIQYEFPMGWQEIEGIHSRTDFDLKGHQEHSGKKMEYFDAQTNER
                     YIPYVVETSAGLDRTILMLLSDAYAEEDVEGDERVVLRFHPAIAPVKVAVLPLVKKDG
                     MPEVAHAIEEDLRSLGLTFYDEKGAIGRRYRRMDEVGTPFCVTVDGDTLADGTVTVRE
                     RDSMEQVRVDKAQVATWLFDRIRAWKPAG"
BASE COUNT          687 a         1530 c         1385 g          707 t
ORIGIN      
        1 ttgccggact gggcgagcgc ggtcgatgcc gcacaggtac ttaggaggat tgcgagaagc
       61 gagacgctcc gaagaatgcc tttcatggca cgatccgcac ctggtgtttc cggggccggc
      121 gcatggaata cgccgcgtat gaccggaata tgaggcttat tgacgtggat gtaaaccatc
      181 cacggggcac tcatttgtgc cccgacgacg caatcggaca agtccctacg cgtacgtagt
      241 ccgatccccg ccgcgtgtcg gtgcctaaag caccctccgg acatcccgcg cgaggggcta
      301 cttcactccc tcgctcatgg ccatcatctt ctcgatcggg gccagtgcgc ggaggcgcaa
      361 ttgttcgtcc atcgtgatct cgggcgcctc gtgcttcatg cacaggtaca gcttctcgat
      421 cgtgttcagg cgcatgtgcg ggcactggtt gcacgcgcac ccgctctcgg gaggcgcggc
      481 cacgaacgtc ttgtccgggt tgtccttccg catctggtgg aggatcccct cctcggtcgc
      541 gacgatgaac gtcgaggcag ggttctcgga ggcgtgccgc cggatctggc tcgtggaccc
      601 cacgaaatcg gcctggcgaa gcaccgcctc ctcgcactcg gggtgcgcga gcacgggcgc
      661 gtccggaaac cggaccttaa gccggacgag cctttcctcg gaaaaggtct cgtgcaccac
      721 gcaaaccccg tcccagagca ccatgtcgcg gccggtttcc ttcaccaggt accgacccag
      781 gttgcggtcg ggcgcgaaga tgatcggctg gtcctccggg atctgccgga tgatgtgcgc
      841 ggcattcgac gacgtgcaga tgatgtcgct gagcgccttg gtcgccgcgg agcagttgat
      901 gtacgagatc acgatgtgat ccgggtacct ctccttgaac gcggcgaaca ggtcgggcgg
      961 gcacgagtcg gcgagcgagc atcccgcgtt caggtccggg agtacgacct tcttccgtgg
     1021 gctcaggatc ttcgcgacct cggccatgaa gtgcacgccc gcgaagacga tcatgtcggc
     1081 gtccgtggcg gcggccttcc gcgagaggcc cagggagtcc ccgatgaagt cggcgatgtc
     1141 ctggatcgcc ggctcctggt aatagtgcgc gagcaggacc gcgttcttct cgcgcttcag
     1201 gcgttcgatc tcctcgtaca ggtccaggcc gggatcgacg ggctcgtcga cgtagccaat
     1261 ggccgggtcc aggatgacgg gaagctgcat gacgatgcgg ttcgggtgcg acgggcgcgc
     1321 acgggaccca atgtcgcgcc gcgacgaacg ttcctaccgc ccgcccgggc gccgcggaat
     1381 cccgcaaatc cttaagaaac cgtcatcaat cgcgcggctt ccacggaatg aaggccgacg
     1441 ttcgccgcgc gtagtccgcg tagccgggct tcgtttcggc gagcgcgcgc tccagcatgg
     1501 cgacgcccga gaccttcacg atgagccacg tgatcacgag cggcccggcg agcgccaccc
     1561 atccacccgg gacggacagg gccatgagcc cgaaccccca ccacaccagc gtctcgccga
     1621 agtagttcgg gtgtcgcgac catcgccaca ggccgcggtc catgacggac cccttcgccg
     1681 tcctcgtcct ccggaaccgt agcatctgcc aatcggcgac cgactcgatc gcgaacccgg
     1741 ccagccagat tgcgatcccc gcgacgtccc actcggtcgg gacgcccgga ccgcgggccg
     1801 cgacctggac gacgagcggc agcgcgacca cgcacaggat gacgccctgg agcaggaaca
     1861 ccttgaagaa gctcctccac caccagcctc ggcccgcctc gcgccgccac ttcgcgtagc
     1921 gggcgtcctc cgctccccgg ccgatcgcca ccgcgaggat gtgccccccg agccggaatc
     1981 cccacgtggc cgtgagcgcg agcacccacg ccacccggcc gcccgacgcg cccacggaga
     2041 cggcggcggc cccggcggcg accaggaagc ccggccccca ggccacgtcc atgatcgacg
     2101 agtcccgcag cgcgaggctc agcagccaca ccagggccgt gtacgcgagg acggctgcga
     2161 ccacacagcc gatgacgccg gcgggcagga atggtaacgt catggtgatc tccggcgagg
     2221 aacctccgtg cgaggcgggc gcttgatccg tgcggaagga ccccgattcg agcctccgga
     2281 ccatgatacg cccgtccgtc ctcttcgtct gtacccacaa ctcggcccgc tcgcaactcg
     2341 ccgaggccct tcttcgcgcc cgtttcggcg accggtacca ggtctactcc gccgggaccg
     2401 tgaagacgcg ggtaaagccc gaggtgctca cggtgctcgc cgagatcggg atcgacgcct
     2461 cggcccaccg gtcgaaaagc gtggacgacc tcggcggccg gtccttcgac tacgtggtca
     2521 ccgtgtgcga ctcggcgaag gaagcgtgcc cgttcgttcc cgccgtccgg gcgcgcacgc
     2581 acgccccgtt cgaggacccg tcagacgtca ccgaaccgcc cggggcgagg ctcgacgcat
     2641 tccgggatgc gcgggagcgg ctcgccgggt ggatcgaccg tgtgttcgcc ggtcctgagc
     2701 ccgatttcga cgcgggcggc tgatcccggg gccgggcgtc atccggccgg cttccacgca
     2761 cggatgcggt cgaacagcca ggtcgccacc tgggccttgt ccacgcggac ctgctccatg
     2821 gagtcgcgct cgcgcaccgt gacggttccg tccgcgagcg tgtccccgtc gaccgtcacg
     2881 cagaagggcg tgcccacctc gtccatccgg cggtaccggc gcccgatcgc tcccttctcg
     2941 tcatagaagg tgaggccgag cgagcggaga tcctcctcga tggcgtgcgc cacctcgggc
     3001 atgccgtcct tcttcacgag cgggagcacg gcgaccttca ccggcgcgat ggccgggtgg
     3061 aaacggagca ccacgcgctc gtccccctcc acgtcctcct cggcataggc atcgctgagc
     3121 agcatcagaa tcgtgcgatc caggccggcg gaggtctcca cgacgtacgg gatgtaccgc
     3181 tcgttcgtct gggcgtcgaa gtactccatc ttcttgccgg agtgctcctg gtgccccttc
     3241 aggtcgaagt ccgtccggga gtggatcccc tcgatctcct gccagcccat cgggaactcg
     3301 tactggatgt cgaatgccgc gtcggcgtag tgcgccagct tctcgtgctc gtgccaccgc
     3361 aggcgggacg cgccgacgcc gttgtccacg tgccactgca tccgcctctc gcgccaggcc
     3421 tcgaaggcct ccatctggtc cccgggcttg acgaaatact gcatctccat ctgctcgaac
     3481 tcgcgcatcc ggaagatgaa ctggcgcgcc acgatctcgt tgcggaacgc cttgccgatc
     3541 tgcgcgatgc cgaagggcac ctgcaggcgc gacgcctccc gcacgttgtg gaagttgacg
     3601 aagatgccct gggccgtctc cggccgcagg tagatcgtgt tcgtctcgcc ggcgagcgcg
     3661 ccgagccggg tctcgaacat caggttgaac tggcgcacct cggtccagtc ggccatcccg
     3721 gagtccggcg acttgatctc ttcccgaaga atcacgtcgt agagcgcctt cgcggggtcc
     3781 tccgcgttca gggcgtccac cagggcgtcg tacacgcggt cggcgtctgc gtccttgccc
     3841 ttcctgcgaa gccggtccac gtgcccctcg atcagctggt cggcccggta ccggatcttc
     3901 gacacgcggt cgtcgatcat cgggtcgttg aacgcgtcca cgtgcccgga cgccttccac
     3961 acggtgggat gcatcaggat cgccgcgtcg agccccgcca cgttctcgtt gcggtagacc
     4021 atcgcgtccc accagcgctg cgcgacgttc cgcttcagct ccacgcccag cggtccgtag
     4081 tcatacgtgg cgccgaggcc gccgtacact tcggacgact ggaagatgaa cccgcggcgc
     4141 ttgctcaggg atacgatttt ctcgaggagg tcggacatgg acggcggctg gtggcgatgc
     4201 tcgggagccg gttcaatccc gaacccgccg cgcgggtccg cccacgccca agaaaatcgg
     4261 ccgcccgccc ccgcccccga gcgcgtgcga agcgaggcgc gcccccgcc
//
LOCUS       DBNHQZ010000382        10604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_654867, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000382 DBNHQZ010000000
VERSION     DBNHQZ010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_654867"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2775
                     /locus_tag="AC16YU_10320"
     CDS             <1..2775
                     /locus_tag="AC16YU_10320"
                     /inference="COORDINATES: protein motif:HMM:NF020243.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6065664.1"
                     /translation="APDPILASVIEIVAAQTGYPVDMLDPELDLEADLGVDTVKQAEM
                     FAAVRARYDIDRDESLQLRDFPTLADVARFVLERLPDSPTPVGGDGVAQLADAPTPAD
                     PGAAIVRRVPVSRVLPPANRYRPTGVSLDRGARVLLFPDSGGTGRALRTRLEKRGVEV
                     LQVEGAPTRDEIETLIARWRQQGPIRGAFWLPALDAEEDVRTADPEASLEALRVRAKL
                     LHATARALYDGLEQGSFFVAATRLDGRCGFGGGAIRAPFGGAVGGFVKALGRERPACL
                     AKVVDFEPGKKSAEIAGRLVDEILNDPSTVEVGYADDRRFGVGLDVRPLPARTGRNAL
                     PDDPVFVVTGAAGSIVSAIVADLAARFRGTFHLLDLSEAPEIDDPDLDAIADDPDGLR
                     RTLFSRLKDAGERATPAVVERLVAGIERRAAARSAIRAVERSGGTARYWPLDLLDRDS
                     VARAVGSITREHKRIDVVLHAAGLEKSRMLPDKEPAEFDLVFDVKTAGYVNLLSAMGR
                     TPVGTAVVFSSIAGRFGNAGQTDYSAANDFLAKVTAALGPETRGITIDWTAWADIGMA
                     SRGSIPTMMARAGIDMLPPALGIPVVGDEIVRGATDGEVVVAQSLGAMLEPRVEDTGL
                     DASGPGPMIDRVLRVHPVDGFVFGADLDPTVQPFLDDHRIDGTPVLPGVMGIEAFVEA
                     ARLLAPDRIPASVESIQFAAPFKFYRDAPRTVEVHARALADGADIVVECRLVGRRRVA
                     SQDADLETVHFAGSVRMRRPADVQPIEAPAHVPDHLDGSAADDLYRVYFHGPAYRVVD
                     SLVRGDGGVSGRLRSGLPPHHLPADRPLQAAPRLIELAFQTAGTVEMRDDLVMGLPSF
                     IRSIRFGPARPVEAGGEIALATRDAAGAFDVVVVRDGEPLLALEGYRTAALPTALPDA
                     AAEVLGALVG"
     gene            2759..3472
                     /locus_tag="AC16YU_10325"
     CDS             2759..3472
                     /locus_tag="AC16YU_10325"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR6065665.1"
                     /translation="MRSSADDIPAGIALERGRLRDVPPLAAWLAAEESAARSALRAEA
                     RRRGWTLGRWVAKRAAMRLAGEPDGADPRRWAVIAAPDGAPEVFLDGAPHPVSISLSH
                     RAGAALAVAAEHPAVVGCDLELLEPRTEAFIDDYLVASERDYVRSAPPGPDRDLRANL
                     AWCGKEAALKVVRTGLRRDTRSMVTGLDAQDAARGGRPDEWHPFRVEDRTDGTVLRAW
                     WRAANGFVEVVALGSLRDA"
     gene            3519..4649
                     /locus_tag="AC16YU_10330"
     CDS             3519..4649
                     /locus_tag="AC16YU_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6065666.1"
                     /translation="MILILQPDTDPGSSEYERLVAHLEGLAPSIEYRVHEVQGSGQRL
                     TEVYLIGDTSALSPDSMRALPCVERVIRISEAYKVLGRHRQDDRPFDFTYRGVRFGQD
                     TLHVFAGLCAVDTPVHVEQMMAALRDHGQVCTRMGAYKPRTSPYSFQGHGKHCLPYVF
                     DLAGRYGIRVVAMEVTHESHVDEIHEALQEAGDPTGVMLQIGTRNTQNFELLKHVGRQ
                     RTYPVLFKRGFGITLDESLNAAEYLASEGNRNVIFCLRGMKTNLADPHRNFVDFAQVP
                     VVKRLTRMPVCIDPSHSVGSRSACPDGILDVMHATAQGVVAGANMVLVDFHPNPAEAL
                     VDGPQALRMNELGLFLEDVRIAREAFLARRQLAARHAGAVAG"
     gene            complement(4653..6299)
                     /gene="pgm"
                     /locus_tag="AC16YU_10335"
     CDS             complement(4653..6299)
                     /gene="pgm"
                     /locus_tag="AC16YU_10335"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HIR6065667.1"
                     /translation="MALHELAGRPAPRSLLVNVPQLVSAYYTERPDPSDPSGRVSFGT
                     SGHRGSSLRGTFNEPQLLAIAQAVAEHRAAAGIGGPLYLGMDTHALSEPAHRTATEVF
                     AANGVQVHVAADGGFTPTPAVSHAILRHNRAGSRGLADGVVITPSHNPPEDGGFKYNP
                     PSGGPADTETTRAIEARANELLAGGCRGVRRLTLREALGSGNVHAFDFVTPYVAALAE
                     VVDLEAVASAGVRIGVDPMGGAGIGYWEPIADRYGLDLDIVNPVVDPTFSFMRVDHDG
                     RIRMDCSSPYAMAGLVELASRFDIAFGNDPDFDRHGIVTPRGGLMNPNHYLAAAVAYL
                     FRNRPGWPEAAGVGKTLVSSSMIDRVAADLGRRLVEVPVGFKWFVEGLLDGSVAFGGE
                     ESAGASFLDRAGGAWSTDKDGIIMDLLAAEMLARTGRDPAEHYRRLTGRFGEPVYARM
                     DAPATGPQKALLSRLSPEDVPGDRLGGDPITAKLTRAPGNGASIGGLKVTTDHGWFAA
                     RPSGTEEIYKIYAESFRGAEHLALIQDEAQRLVRTVFEAA"
     gene            6450..7205
                     /gene="ppgK"
                     /locus_tag="AC16YU_10340"
     CDS             6450..7205
                     /gene="ppgK"
                     /locus_tag="AC16YU_10340"
                     /EC_number="2.7.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017779123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0047330 - polyphosphate-glucose
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--glucose phosphotransferase"
                     /protein_id="HIR6065668.1"
                     /translation="MHVLGIDIGGSGIKGAPVDISTGSLSADRYRIQTPQPATPQKVA
                     KAVGRIAAHFEWKGPIGCTLPARVQHGVAHTATNIHKSWIDTAVDELFSAQTGLPVRV
                     LNDADAAGVASMAFGAGKDRTDLVLMLTVGTGIGSALFVNRQLVPNTELGHLDLDGRN
                     AELFCSDRARKRDGLSWKQWARRFQVYLDTVEFLFAPDLIILGGGISRPSKVSKYLRY
                     LSTRAELVPSILENEAGIIGAAYAARDLLARTG"
     gene            complement(7257..7778)
                     /locus_tag="AC16YU_10345"
     CDS             complement(7257..7778)
                     /locus_tag="AC16YU_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6065669.1"
                     /translation="MTRFLPLMLLVAVAACSGAGRVAETGTRPDDAVRVDTVAWETFE
                     IEPFRDEPAVISTDPEHDVPAPLMDNRADAGIEVTVDGFRVQLFSSLEEEEAAAAEEE
                     ARTLWEETIAAAEPGADLPADLQVYRLFRQPYYRIRAGDFTDREEADRVALIFASRFE
                     GALVVPDQVTVRR"
     gene            complement(7784..8440)
                     /gene="deoC"
                     /locus_tag="AC16YU_10350"
     CDS             complement(7784..8440)
                     /gene="deoC"
                     /locus_tag="AC16YU_10350"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR6065670.1"
                     /translation="MIDHTALKPETTESDVATLCDEAERYCFASVCINPGWVAYAAER
                     LRGTPIRVCTVVGFPLGATTTAVKALETETAVRDGATEIDMVLNVGRLKSGHTDYVED
                     DIRAVVTAARRAGAATVKVILETALLTDQEKVVACVLARNAGADFVKTSTGFSKGGAT
                     AGDVALMRRVVGPSMGVKASGGVRSRADAERMIAHGATRIGASASVEIIQDRETSSSY
                     "
     gene            complement(8700..9638)
                     /locus_tag="AC16YU_10355"
     CDS             complement(8700..9638)
                     /locus_tag="AC16YU_10355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6065671.1"
                     /translation="MHLVAIINPAAAHGRVGRRIEEIRALFRSRSAGTAEIRVTEGRG
                     HATELAHAVADRDVVVAVGGDGTVHEVALGLLGAEGRAALGIVPIGTGNDLAHALGYR
                     GRPADAVAALDRSRRATMDCGMAVLDGNTATPVPFFNNVGLGFDALASFHAVAYKHLP
                     GPLAYGAGVLRALTSWTGPQARIEVDGAVESIPLMFATVANGTRTGGMFRLTPGASLF
                     DGVLDVCTVRYVRPARALMVAPRTLNGSHVRLDEVHMRTGVRIRIDVSEAVPVHADGE
                     IVSRGCRRMDCWVVAGALPVLVPSRDRAGRTPRKRT"
     gene            9731..>10604
                     /locus_tag="AC16YU_10360"
     CDS             9731..>10604
                     /locus_tag="AC16YU_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6065672.1"
                     /translation="MTGRGQALIALLLATLGCGPAGTPTGRPVVDDLGRTIVLSGPVE
                     RVVTLAPSVTELAFEAGASALVVGVTDADDHPDAVTRLPRVQALPVDHEAVVALRPDL
                     VLASDEVNDPRDADRLAALGIPTYFVRIGSLGDVSRVVRNLGTLLGTGRAAREAADSL
                     DARLGALARPPSGDPPDVLLLVGDATLFSFGRGSYVHEMVSAAGGRSITAGFEMRAPV
                     LSEEFVITAAPDVIIGAFGTGYDPARLLELHPAWQDVPAVRDGRVHSIDPDLVLRPGP
                     RLVLGVEAIRGVLDP"
BASE COUNT         1459 a         3923 c         3694 g         1528 t
ORIGIN      
        1 gccccggacc ccatcctggc gagcgtcatc gagatcgtcg ccgcgcagac cggatacccg
       61 gtggacatgc ttgatccgga actcgacctt gaagccgacc tgggcgtcga cacggtgaag
      121 caggccgaga tgttcgcggc cgtccgggcc aggtacgaca tcgatcgcga cgagtccctg
      181 cagctcaggg acttcccgac gctcgccgac gtggcccgat tcgtcctcga gcgccttccc
      241 gactccccga ctcccgtggg tggtgacggc gtggcacagc tcgccgacgc cccgaccccc
      301 gccgatcccg gcgccgcgat cgtccgccgg gtgcccgtga gccgggtcct tccgcctgcc
      361 aaccgctacc gcccgaccgg cgtctcgctg gaccgcgggg cgcgcgtcct cctcttcccc
      421 gactccggcg ggaccggacg ggcgctcagg acgcggctcg agaagcgggg cgtggaggtc
      481 ctccaggtcg aaggcgcgcc gacgcgcgac gagatcgaga ccctcatcgc ccggtggcgg
      541 cagcagggac cgatccgcgg cgcgttctgg ctccccgccc tcgacgcgga ggaggatgtc
      601 cgcacggcgg atcccgaagc gagcctggag gccctccggg tccgggccaa gctgctccat
      661 gcgacggccc gggcactgta cgacggcctg gagcagggct cgttcttcgt cgccgccacg
      721 cgcctcgacg gccggtgcgg cttcgggggc ggggccatcc gcgccccctt cggtggcgcg
      781 gtgggcggat tcgtcaaggc cctcggacgg gagcgccccg cgtgcctggc gaaggtcgtg
      841 gacttcgagc ccgggaagaa gagtgccgag atcgccgggc ggctcgtcga cgagatcctg
      901 aacgacccgt ccaccgtgga ggtcgggtat gccgacgacc ggcgcttcgg cgtcgggctg
      961 gacgtgcgcc cgctgccagc gcgaaccggc cgcaacgcgc ttccggacga ccccgtcttc
     1021 gtcgtgaccg gcgccgccgg aagcatcgtc tcggccatcg tcgccgacct ggcggcgcgc
     1081 ttccgcggga ccttccacct gctcgacctt tccgaggccc cggagatcga cgatccggac
     1141 ctggacgcca tcgccgacga tcccgacggg ctgcggagga cgctcttctc gcgcctgaag
     1201 gatgccggcg agcgggcgac ccccgctgta gtggagcgcc tggtggcggg aatcgagcgc
     1261 cgcgcagctg cccgttcggc cattcgggcc gtcgagcggt ccggcggaac ggcgaggtac
     1321 tggccgctcg acctcctgga ccgcgactcg gtggcacggg ccgtcgggag catcactcgg
     1381 gagcacaagc gcatcgacgt cgtgctccac gcggctggcc tggagaagag ccgcatgctc
     1441 ccggacaagg agccggccga gttcgacctg gtgttcgacg tcaagacggc cgggtacgtg
     1501 aaccttctct cggcgatggg ccgcaccccg gtcgggacgg ccgtggtgtt cagctcgatc
     1561 gccggccggt tcggaaacgc gggacagacg gactacagcg cggcgaacga cttcctggcg
     1621 aaggtcaccg ccgcgctcgg accggagacg cgcggaatca cgatcgactg gaccgcctgg
     1681 gccgatatcg ggatggcctc gcggggctcg attccgacga tgatggcgcg cgcgggcatc
     1741 gacatgcttc cgcccgccct cgggattccg gtcgtcggcg acgaaatcgt ccgcggcgcg
     1801 acggacggcg aggtcgtcgt cgcgcagtcg ctgggcgcca tgctggagcc gcgcgtcgag
     1861 gataccggcc tggatgcttc gggccccggc ccgatgatcg atcgcgtgct ccgcgtgcat
     1921 cccgtggacg gattcgtctt cggcgcggat ctcgatccca ccgtacagcc cttcctggac
     1981 gatcaccgca tcgacggcac gccggtgctt ccgggtgtca tgggcatcga ggcgttcgtc
     2041 gaggccgccc ggctgctggc gcccgacagg atcccggcgt cggtcgagtc gatccagttc
     2101 gcggccccgt tcaagttcta ccgggacgcg ccgaggacgg tcgaggtcca cgcccgggcg
     2161 ctggccgacg gcgccgacat cgtcgtcgag tgccggctgg tgggccgcag gcgcgtggcc
     2221 tcccaggacg ccgacctcga gaccgtccac ttcgcgggca gcgtgcgcat gcgccggccg
     2281 gccgacgtgc agccgatcga ggcgccggcc cacgtgccgg atcacctgga cggatcggcg
     2341 gccgacgacc tgtaccgcgt ctacttccat ggccccgcct accgggtggt cgactcgctg
     2401 gtccgtggcg acggcggcgt gtcggggcgc ctccggtcgg gactcccccc gcaccacctg
     2461 ccggccgatc gaccactcca ggcggcgccc cggctgatcg aactcgcatt ccagacggcg
     2521 ggcaccgtcg agatgcgtga cgacctggtg atgggtctgc cgtccttcat ccgctcgatc
     2581 cggttcgggc ccgcgcggcc ggtcgaggcc ggaggcgaga tcgctctcgc cacccgcgac
     2641 gcggcagggg cgttcgacgt cgtggtcgtc cgggacggcg agccgctcct ggcgctcgag
     2701 ggctatcgaa cggcggccct cccgacggcc ctgcctgacg cggcggcgga ggtcctgggt
     2761 gcgctcgtcg gctgacgaca taccggccgg catcgccctg gaacggggcc gcctgcggga
     2821 cgtgccgccg ctggccgcgt ggctggcggc cgaggagtcg gccgcccggt cggcgctccg
     2881 ggccgaggcg aggcgacgcg gctggaccct gggtcgctgg gtggccaagc gcgccgcgat
     2941 gaggctggcg ggcgagccgg acggggcgga tccccgccgg tgggcagtga tcgcggcacc
     3001 ggacggggcg cccgaggtgt tcctcgacgg cgccccccat cccgtgtcga tcagcctgtc
     3061 gcaccgcgcc ggggcggccc tcgccgtggc cgcggagcac ccggccgtgg tcgggtgcga
     3121 cctggagctc ctggagcccc ggaccgaggc cttcatcgac gactacctgg tcgcgtcgga
     3181 gcgggattac gtgcgctcgg cgccgcccgg ccccgaccgg gacctgaggg ccaacctggc
     3241 ctggtgcggc aaggaggccg cgctgaaggt ggtccggacg gggctgcgac gggacacgcg
     3301 gagcatggtc accgggctcg acgcgcagga cgccgcacgc ggaggcaggc cggacgagtg
     3361 gcatccgttc cgcgtcgagg accggaccga cggcaccgtg ctccgggcct ggtggcgtgc
     3421 cgcgaacggc ttcgtcgagg tcgtcgccct gggatcgctc cgggacgcgt gagcgcggtc
     3481 tgcgtatctt gcgcgcctgc catgcagcgc gaagcaccgt gatcctgatc ctgcagcccg
     3541 acaccgaccc cgggtcctcc gaatacgagc gactcgtggc gcacctcgag ggcctcgccc
     3601 cgtccatcga gtaccgagtc cacgaggtgc agggcagcgg gcagcgcctg accgaggtct
     3661 acctcatcgg cgacacgagc gcgctgtcgc cggactccat gcgcgcgctt ccatgcgtgg
     3721 agcgcgtgat ccggatctcg gaggcctaca aggtgctcgg gcggcatcgg caggacgacc
     3781 ggccgttcga cttcacgtac cgcggcgtcc gcttcggcca ggacaccctc cacgtgttcg
     3841 ccgggctctg cgccgtcgac acgcccgtgc acgtcgagca gatgatggcc gccctcaggg
     3901 accacggcca ggtctgcacg cgcatgggcg cctacaagcc gcgcacgagc ccgtacagct
     3961 tccagggcca cgggaagcac tgcctgccgt acgtgttcga cctcgcgggc cggtacggca
     4021 tccgggtggt ggccatggaa gtcacgcacg aatcgcacgt ggacgagatc cacgaggccc
     4081 tgcaggaggc cggcgatccc accggcgtga tgctccagat cggtacccgc aacacgcaga
     4141 acttcgagct cctcaagcac gtggggcgcc agcggactta ccccgtcctg ttcaagcgcg
     4201 ggttcggcat cacgctcgac gagagcctga acgcggccga gtacctcgcc agcgagggga
     4261 accggaacgt gatcttctgc ctgcggggca tgaagaccaa cctcgccgat ccgcaccgca
     4321 acttcgtcga cttcgcccag gtgccggtcg tcaagcggct gacgcgcatg ccggtctgca
     4381 tcgatccgtc ccactcggtg ggctcccgga gcgcgtgccc cgacggcatc ctggacgtca
     4441 tgcacgccac ggcgcagggc gtcgtagcgg gcgccaacat ggtcctcgtg gatttccacc
     4501 cgaatcccgc ggaggccctg gtggacggtc cccaggcgct ccggatgaac gagctcgggc
     4561 tcttcctcga ggacgtccgg attgcccgcg aggcgttcct ggcccggcgg cagctggccg
     4621 cccggcacgc cggggccgtg gccggctgat tcctacgcgg cttcgaagac cgtccggacc
     4681 agccgctgcg cctcgtcctg gatcagggcc aggtgttcgg ccccgcggaa gctctcggcg
     4741 taaatcttgt atatctcctc ggtaccggac gggcgcgcgg cgaaccagcc gtggtccgtc
     4801 gtgaccttga ggccgccgat ggacgcgccg ttcccgggtg cccgcgtgag cttggccgtt
     4861 atcggatccc ctcccaggcg gtccccggga acgtcctccg gcgagagcct ggagagcagc
     4921 gccttctgcg gccccgtggc cggggcgtcc atcctcgcgt acaccggctc cccgaaccgc
     4981 ccggtgagtc ggcgatagtg ttcggccggg tcccggcccg tgcgggccag catctcggcc
     5041 gccagcaggt ccatgatgat cccgtccttg tcggtggacc acgcgccgcc cgcccggtcc
     5101 aggaaggagg cccccgcgct ctcctccccc ccgaaggcta cagagccgtc gagcagtccc
     5161 tccacgaacc acttgaagcc caccgggacc tcgaccaggc ggcgtcccag gtcggccgcc
     5221 acccggtcga tcatcgagct cgacacgagc gtcttcccga cgcccgccgc ctcgggccag
     5281 cccggccggt tccggaacag gtacgccacg gcagcggcga ggtaatggtt ggggttcatg
     5341 aggcccccgc ggggcgtcac gatgccgtgg cggtcgaaat ccgggtcgtt cccgaacgcg
     5401 atgtcgaacc gggaggcgag ctcgaccagg ccggccatcg cgtacggcga ggagcagtcc
     5461 atgcggatcc ggccgtcgtg gtccacgcgc atgaacgaga aggtcggatc caccacgggg
     5521 ttcacgatgt ccaggtccag gccgtaccgg tccgcgatcg gctcccagta tccgattccg
     5581 gccccgccca tcggatcgac gccgatccgg acgccggccg aggcgacggc ctcgaggtcg
     5641 accacctccg cgagggcggc cacgtagggc gtcacgaaat cgaaggcgtg cacgttgccc
     5701 gagcccaggg cctcgcggag cgtgagtcga cgcacgcccc ggcagccccc ggcgagcagc
     5761 tcgttcgccc gcgcctcgat ggcccgcgtg gtctccgtgt ccgccggccc gcccgagggc
     5821 gggttgtact tgaaaccccc atcctccggg ggattgtgcg acggcgtgat cacgaccccg
     5881 tcggcgaggc ccctcgaccc ggcgcggttg tgccgcagga tcgcgtgcga gacggccggt
     5941 gtgggcgtga agcccccgtc cgccgcgacg tggacctgca cgccgttcgc cgcgaacacc
     6001 tcggtcgcgg tgcggtgcgc gggctcggag agggcgtgcg tgtccatgcc caggtacagg
     6061 ggaccgccga tccccgccgc ggcgcggtgc tccgcgacgg cctgggcgat cgcgaggagc
     6121 tgcggctcgt tgaacgtgcc ccgcagcgac gatccgcgat gtccggacgt gccgaacgag
     6181 acgcgcccgg acggatcgga ggggtcgggg cgctccgtgt aataggccga gaccagctgc
     6241 gggacgttca cgagcaggga gcgcggggcc ggtcggccgg cgagttcgtg gagagccatg
     6301 gtgcagggtt cggtggggcc gggcgaaaaa tacgtgttat ccacgccgct gccccggcca
     6361 ccgggtgaag atccgggccg ggtcgccgat tgctccttcc cccgggtccg cgtaagttcg
     6421 cgccgggcac accatcccgc ggcggaaccg tgcacgtact cggcatcgac atcggcggct
     6481 cgggcatcaa gggagccccg gtcgacattt ccaccggctc cctgtcggcc gaccggtacc
     6541 ggatccagac cccgcagccc gccacgccgc agaaggtggc gaaggcggtc gggaggatcg
     6601 ccgcgcattt cgagtggaag ggtccgatcg gctgcacgct gcccgcgcgc gtgcagcacg
     6661 gggtggccca tacggccacg aacatccaca agtcgtggat cgacaccgcc gtcgacgagc
     6721 tgttctccgc ccagaccggc ctccccgtcc gcgtgctgaa cgacgccgat gccgcggggg
     6781 tcgcctccat ggcgttcgga gccggcaagg accgcacgga cctcgtcctg atgctgaccg
     6841 tcggaaccgg catcgggtcc gcgctcttcg tcaaccgcca gctcgtcccg aacacggagc
     6901 tcggccacct ggacctggac ggccggaatg ccgagctctt ctgctccgac cgggcccgca
     6961 agcgcgacgg cctgtcgtgg aaacagtggg cgaggcggtt ccaggtgtac ctggacacgg
     7021 tggagttcct cttcgccccg gatctcatca tcctgggtgg cggcatatcc cgcccctcca
     7081 aggtctccaa gtacctccgg tacctctcga cgcgggccga actcgtgccc tcgatcctcg
     7141 agaacgaggc gggcatcatc ggggccgcct acgcggcgcg cgacctgctg gcccgaaccg
     7201 gctgacgcgg cgggaatgat ccggccccca ccggcgacgt ccgcgatccg cgcgcgctac
     7261 ctccggaccg tgacctggtc ggggaccacc agcgccccct cgaaccggct cgcgaagatg
     7321 agcgccaccc ggtccgcttc ctcccggtcg gtgaaatcgc ctgcgcggat gcggtagtac
     7381 ggttgacgga acaggcgata gacctgcagg tcggccggca ggtccgctcc gggctcggcg
     7441 gcggcgatcg tctcttccca gagggtccgc gcctcttcct cggcggccgc ggcttcctcc
     7501 tcctcgagcg acgagaagag ctgtacgcgg aatccgtcca cggtgacctc gatgccggca
     7561 tccgcgcggt tgtccatgag cggcgccgga acgtcgtgct ccggatcggt cgatatcacc
     7621 gccggctcgt cgcggaacgg ctcgatttcg aacgtctccc acgcgaccgt gtccacgcgc
     7681 acggcgtcgt ccggccgcgt cccggtctcc gcgacgcgcc cggcgcccga gcacgccgcg
     7741 accgccacga ggagcatcag gggaagaaac ctggtcatgt gattcagtag ctgctgctcg
     7801 tttccctgtc ctggatgatc tcgaccgagg cgcttgcgcc gatgcgggtc gcgccgtgcg
     7861 cgatcatccg ctcggcgtcg gcacgggacc ggacgccccc cgacgccttc acgcccatcg
     7921 aggggccgac cacccttcgc atcagggcca cgtccccggc cgtcgcgccg cccttcgaga
     7981 acccggtcga cgtcttcacg aaatccgccc cggcgttccg ggccaggacg cacgcgacga
     8041 ccttctcctg gtccgtcagc agggccgtct ccaggatcac cttgacggtc gccgctcccg
     8101 cccgtcgggc ggcggtcacc accgcccgga tgtcgtcctc gacgtagtcg gtgtgccccg
     8161 acttcagcct gccgacgttc agcaccatgt cgatctccgt ggcgccgtcg cgcacggccg
     8221 tttccgtctc cagcgccttg acggcggtgg tcgtggcccc gagcgggaat ccgaccaccg
     8281 tgcacacgcg gatcggcgtt ccccggagcc tctcggccgc gtaggccacc cagcccgggt
     8341 tgatgcagac cgacgcgaag cagtaccgct cggcctcgtc gcacagcgtc gcgacgtccg
     8401 attcggtggt ttccggcttg agcgccgtgt gatcgatcat ccgcgcgagc ggcggcgcca
     8461 ggtcgcaggc cgagatgccg tggcgctccg gctggtcggc gcacacgccg aaacggcacg
     8521 ccccgaccgt aaccatgtga tccagcgccg tctcgacgcc gtgcgccggc gccgcgacgc
     8581 ccgcggacgc cgggaccggc cgaccggccg agaattcctc ggccagctgc cgcacgatgc
     8641 gatcgcgctc tgcctcgttc atgccgcaag atacaagcgg cacggatcga atgaccgcat
     8701 caggtccgtt tccgcggggt gcgccctgcc cggtcccggg acggaaccag cacgggcagc
     8761 gccccggcca cgacccagca gtccatgcgc ctgcagcctc gcgatacgat ctccccgtcc
     8821 gcgtgaaccg gaaccgcctc cgacacgtcg atgcgtatcc gaaccccggt tctcatgtgg
     8881 acctcgtcga gccggacatg gcttccgttc agcgtgcgcg gcgcgaccat gagcgcccgc
     8941 gcgggacgca cgtagcgcac cgtgcacacg tccagcacgc cgtcgaagag cgaggcgccg
     9001 ggcgtaagcc ggaacatgcc ccccgtgcgc gtcccgttgg cgaccgtcgc gaacatgagc
     9061 gggatcgact ccacggctcc atcgacctcg atccgggcct gcggacccgt ccacgacgtg
     9121 agcgccctga gcacgcccgc tccgtacgcc agcggaccgg gcaggtgctt gtacgcaacg
     9181 gcatggaacg aggccagcgc gtcgaatccc agccccacgt tgttgaagaa ggggacgggc
     9241 gtggcggtgt tgccgtcgag tacggccatg ccgcagtcca tcgtggcgcg gcggctccgg
     9301 tcgagcgccg cgacagcgtc ggccggcctg ccgcggtacc cgagggcatg cgccaggtcg
     9361 ttccccgtgc cgatcggcac gatccccagg gccgcccgcc cctccgctcc cagcagcccg
     9421 agcgcgacct cgtgaacggt gccgtctccg ccaacggcca ccacgacgtc ccggtcggca
     9481 accgcgtggg ccagttccgt cgcgtggccc ctcccctcgg tcacccggat ctcggccgtt
     9541 ccggcagagc gcgaccggaa cagggcccgg atctcctcga tccggcgccc caccctcccg
     9601 tgggcggccg cggggttgat gatggcgacg agatgcacgt tccgggcgtc cgtggtccgc
     9661 gaacatacgc acccggaacc ggttcaccgg cgtcctctat cttgccccct ttcgaattcg
     9721 gaagagcccc gtgaccggcc gaggacaagc cctcattgct ctgctcctgg cgaccctggg
     9781 atgcggcccc gccggcacgc cgaccggtcg gcccgtcgtc gacgacctgg gccgcacgat
     9841 cgtcctctcg ggcccggtcg agcgcgtggt caccctggcg ccgagcgtca ccgagctcgc
     9901 gttcgaggcc ggcgcctcgg ccctcgtcgt cggggtcacg gacgcggacg accaccccga
     9961 cgccgtcacc cgtcttccgc gggtccaggc gctcccggtc gaccacgagg cggtcgtcgc
    10021 gctgcggccc gacctggtcc tcgcgtccga cgaggtgaac gatccgcgcg acgcggaccg
    10081 tctcgcggcg ctcgggattc ccacctattt cgtgcggatc ggatcgctcg gcgacgtctc
    10141 cagggtcgtc cggaacctcg ggacgctcct gggcaccggg cgcgcggccc gcgaagccgc
    10201 ggactcgctc gacgcgcgcc tcggagcgct cgcccggccg ccatccggcg acccgccgga
    10261 cgtcctcctg ctcgtcggcg atgccacgct attctcgttc ggccgcggca gctacgtgca
    10321 cgagatggtg tcagcggccg gaggccggtc gatcacggcc ggcttcgaga tgcgggcccc
    10381 ggtcctgagc gaggaattcg tgatcaccgc ggctccggac gtcatcatcg gggccttcgg
    10441 gaccggttac gacccggctc gcctcctcga gctgcatccg gcctggcagg acgtgcccgc
    10501 ggtccgcgac ggccgggtgc actccatcga cccggacctg gtccttcggc ccgggccgcg
    10561 gctcgtgctg ggagtcgagg cgatccgggg agtcctcgac ccgc
//
LOCUS       DBNHQZ010000383         5949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3092507, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000383 DBNHQZ010000000
VERSION     DBNHQZ010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3092507"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="AC16YU_10365"
     CDS             complement(<1..702)
                     /locus_tag="AC16YU_10365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6065673.1"
                     /translation="MPSAFVTGGTGFVGSHLAEELLGRGYEVRCLVRSEPKWLSDLDV
                     TLVRGSLSRPETLREAVADADFVFHVGGLTRARTWAAFEEANVADTLRLLDLLRGRAA
                     PPKSVLVTSSLAAVGRGGSDVADESTPLRPVSMYGRSKAVMEAGLEPYRAAMPLVVVR
                     PPAVYGPRDTDVLTFFRSVARGICPIVGRGDVPAVSLVHVRDLVRGMADAAESPVAAG
                     RTYFVGSDRPYSWGEV"
     gene            complement(708..2108)
                     /gene="rlmD"
                     /locus_tag="AC16YU_10370"
     CDS             complement(708..2108)
                     /gene="rlmD"
                     /locus_tag="AC16YU_10370"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR6065674.1"
                     /translation="MKKGTTFEARIEKFADRGKSLARIDGYVVFFPGGVPGDRVRATL
                     VRSTSRHGEARVDELIEPSPLRTEPRCRYFGTCGGCKWQHVRYGAQLEAKAQSVAEAF
                     EHHGGFEHPVVHPALGSESEWFYRNKMEFSFSAERWLTREEIASGERFDLGFALGLHA
                     PGHFNKVIDLTECHLQSPLSVELVNGFRRLALDRGWDAWHLRRHQGYLRHLVIRQALH
                     TGDVMVNLVTSRLDEERNEEVAAFLRTGFPQVTTFVNGVHSGVAQTAFGESLHTVFGP
                     GLVRDRIGPYTFEIGPNAFFQTNTAQAERLYEVAKSYARLEPDDLVYDLYCGAGSISL
                     FVAGDVRQVVGVELIEDAVVNARANATANGVGNVVFETGDMLKLFTPDFVRKHGRPDV
                     LIVDPPRAGMHPKVVRQIAELRPGRFVYVSCNPLTQARDLAALADAYSLDELQPVDLF
                     PHTYHVENVARLTARS"
     gene            complement(2112..2723)
                     /locus_tag="AC16YU_10375"
     CDS             complement(2112..2723)
                     /locus_tag="AC16YU_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6065675.1"
                     /translation="MPSAFRLASALRTLRARFARRREDDEKRAVADLLADVPLFSSLK
                     RGMLLDLAEAVHERTYRRDEFLFYQGDPGIGLFVIRSGGVRLLVEDAEGAMHEIRTAA
                     EGDVLGIEGVIGDGSLRRRESAQATGDTRVIGIFSPEYRTLQRRHPKTGAAIAGLLAR
                     HAAAALGELQALLAERGDAVAAGTLADEAVGRAATRLRQMQGM"
     gene            complement(2736..5186)
                     /locus_tag="AC16YU_10380"
     CDS             complement(2736..5186)
                     /locus_tag="AC16YU_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6065676.1"
                     /translation="MPSFRPLLPVLCILTVLAAPAGRASAQTPIGGERTVRISDVEFE
                     GRLVFPEEQLRLNVRTRANRHLLGIPGATWWLWMYRLGDSIGGSVGRGIRSSGEPPAI
                     LDQRVVDADVERLRLFYVQEGFRSAFVAARIDSTERGDRARVRFVVEPGAPTWIRDLS
                     FRGVGALSDEHRDRLVEGSLVALAPVPDSVRTTTRRAVGQRYSEPLLLEEGRRILEFL
                     RRAGYAAVTRDSIHAVIVPARPDSFDVILDIQPGMRYRFGNVAYSVTGIEASPEPRAD
                     TIRTAAPADSIPGGRITASFTRERRMSSGLLMRTLQFRPGEWYDQERLEATKRRLDAT
                     GVFAFTDFQTDLADTTRARPGGDPRIPLTFRLQTRPRHQIRLQTFMLQRSGALADSDN
                     EIGMGLGTTYSNLNTFGEGEQFRLQSTGTVAADLGGPGGFTSAQWEVSASLTYPYLTF
                     PFARLERWLGLYDARTQLSVSLLAARRDALRLVLRGRGGARYRLEMRHNPTLTSLVDL
                     LDLTVSSPDTLAGFSEIFLDDVLAAIDDPIQRGQIVEDYTEPQINNALRYTLRSARVD
                     PFRRSDGYSYEGSVELGGNLPYLLDRYVLTPGELEGSLPGLPFFRGRGSSGRLLYRQY
                     VRLVGDFRRYIRLDPGSVLAWKAFVGVAHPTGEADLIPFDRRFYSGGANSVRAWRLRQ
                     LGPGSASFVPDVDSVTTGTTNLLGGEIKLEASIEFRRTVIRRLLAADWILAVFADAGN
                     VWSGPRNPGIEGGRFRFDTFYREIGVGSGFGVRLAWEYLIVRLDFAYKVHDPLRRSVF
                     LPDGFNDPVVQFGIGHTF"
     gene            complement(5274..5492)
                     /locus_tag="AC16YU_10385"
     CDS             complement(5274..5492)
                     /locus_tag="AC16YU_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HIR6065677.1"
                     /translation="MYWTLELASHLEDAPWPATRLELIDYAERTGAPSEVIENLRAME
                     DDGEPYESIEEVWPDFPTADDDFYYEDE"
     gene            complement(5632..>5949)
                     /locus_tag="AC16YU_10390"
     CDS             complement(5632..>5949)
                     /locus_tag="AC16YU_10390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6065678.1"
                     /translation="VQEVGRGNGFGNYVKIEHRDAGYVTLYAHLSRFADGLRAGRPVA
                     RGELIAFSGNSGLSKAPHLHYEVRDLTGRALNPVYFFAPSMTPVAYQKLLRQSEQGTI
                     SFD"
BASE COUNT          956 a         2030 c         2100 g          863 t
ORIGIN      
        1 gacctccccc catgaatagg ggcggtcgct tccgacgaag tacgtccggc cggcggcgac
       61 cggggattcg gccgcatccg ccatgccccg cacgaggtcg cgcacgtgca ccaggctcac
      121 ggcgggcacg tcgccgcggc ccacgatggg acagatgccg cgcgccacgg accggaaaaa
      181 ggtgaggacg tccgtgtccc gcggaccata cacggcgggc gggcgaacga ccacgagagg
      241 catcgccgcc cggtagggtt cgagcccggc ctccatgacc gccttggacc gcccgtacat
      301 gctgaccggc cggagcgggg tggactcgtc cgcgacgtcc gatccgccgc ggcccaccgc
      361 ggccaggctc gacgtgacga ggaccgactt cggtggcgcg gcccgcccgc gcaggaggtc
      421 gagcaggcgc agcgtgtccg cgacgttcgc ttcctcgaac gccgcccacg tgcgggcgcg
      481 cgtgagcccg ccgacgtgga acacgaagtc ggcgtcggcg acggcctccc gcagcgtctc
      541 cggccgggac agggatccgc ggaccagcgt gacgtccagg tccgagagcc acttcggctc
      601 cgagcggacc aggcagcgca cctcgtagcc gcggccgagc agttcctcgg ccaggtggct
      661 gcccacgaag cccgtcccgc cggtcacgaa ggcgctgggc atggcgttca ggaccgggcg
      721 gtgagacggg ccacgttctc gacgtggtag gtgtgcggga agaggtccac gggctgcagc
      781 tcgtccagcg agtaggcgtc ggcgagggcc gcgagatccc gggcctgcgt cagcggattg
      841 cagctcacgt acacgaagcg cccgggccgc agctcggcga tctgccgcac gaccttcgga
      901 tgcattccgg cgcggggcgg atcgacgatg agcacgtccg gacgcccgtg cttccgcacg
      961 aagtcgggcg tgaagagctt cagcatgtcg ccggtctcga agacgacgtt cccgacaccg
     1021 ttcgccgtcg cgttggcccg cgcgttcacg acggcgtcct cgatgagctc gacacccacg
     1081 acctgccgca cgtcaccggc cacgaacagc gagatggagc cggctccgca gtacaggtcg
     1141 tacacgaggt cgtcgggctc gaggcgggca taggacttcg cgacctcgta gagccgctcg
     1201 gcctgcgcgg tgttcgtctg gaagaacgcg ttcgggccga tctcgaaggt gtacgggccg
     1261 atacggtcgc ggaccagccc ggggccgaag accgtgtgca gcgattcgcc gaaggcggtc
     1321 tgcgcgacgc ccgaatggac gccgttcacg aaggtggtca cctgggggaa accggtccgg
     1381 agaaaggcgg cgacctcctc gttgcgttcc tcgtcgagcc ggctcgtcac gaggttcacc
     1441 atgacgtcgc cggtatggag ggcctgccgg atcacgaggt ggcgcaggta gccctggtgc
     1501 cgccggagat gccaggcgtc ccagccccgg tccagggcca gccggcggaa cccgttgacc
     1561 agctccacgc tgagcggact ctgcaggtgg cactcggtga ggtcgatgac cttgttgaag
     1621 tggccgggcg cgtgcaggcc gagggcgaag cccaggtcga atcgctcgcc ggaggcgatt
     1681 tcctcgcgtg tgagccaccg ctccgcgctg aaggagaact ccatcttgtt ccggtagaac
     1741 cactcgctct ccgagccgag cgcgggatgg accaccggat gctcgaatcc gccgtggtgc
     1801 tcgaacgcct cggccacgga ctgcgccttg gcctcgagct gagccccgta gcggacgtgc
     1861 tgccacttgc agcctccgca cgtgccgaag tagcggcatc gcggctcggt ccgcagcggg
     1921 ctcggctcga tcagctcgtc gacccgcgcc tccccgtgcc ggctcgtcga gcgcacgagc
     1981 gtggcccgga cccggtcccc gggcacgccg ccgggaaaga acacgacgta gccgtcgatc
     2041 cgcgcgagcg acttgccccg gtcggcgaat ttctcgattc gtgcctcgaa ggtcgttccc
     2101 tttttcatgc gtcacattcc ctgcatctgt cggagacggg tggcggcgcg gccgacggcc
     2161 tcgtccgcga gcgtgccggc cgccacggcg tcgccccgtt cggcgagcag cgcctgcagc
     2221 tcgccgagcg cggcggcggc atgtcgggcc aggagcccgg cgatcgcggc gccggtcttc
     2281 gggtggcggc gctggagggt gcggtactcg gggctgaaga tcccgatcac gcgcgtatcg
     2341 ccggtcgcct gagcggactc gcggcgccgg agggatccgt cgccgatcac cccctcgatc
     2401 ccgagcacat cgccttcagc ggctgtccgt atctcgtgca ttgcgccctc ggcgtcctcc
     2461 acgaggagcc gcaccccgcc cgaccgtatg acgaaaagcc cgatgcccgg gtccccctgg
     2521 tagaagagga actcgtcccg ccggtaggtg cgctcgtgga cggcctccgc gagatccagg
     2581 agcattccgc gcttcaggga cgagaacagg gggacgtccg cgagcaggtc ggccaccgcc
     2641 cgcttttcgt cgtcctcccg gcggcgcgca aagcgcgcgc gcagggtgcg aagggcggaa
     2701 gcgaggcgaa aggcggaggg catggcagag caccttcaga acgtatggcc gatgccgaac
     2761 tggacgacgg gatcgttgaa tccgtcggga agaaacacgc tccgtctcag cggatcgtgc
     2821 accttgtacg caaagtcgag ccgaacgatc aggtattccc aagcgagcct gacgccgaag
     2881 cccgatccga cgccgatttc acggtaaaac gtgtcgaagc ggaagcgacc gccctcgatt
     2941 cccgggttgc gcggccccga ccagacgttc ccggcatccg cgaacaccgc caggatccag
     3001 tcggccgcga gaagcctgcg gatgaccgtc cgccggaact cgatggaggc ctccagcttg
     3061 atctcgccgc cgagcaggtt tgtcgtgccc gtggtcacgg agtcgacgtc cgggacgaag
     3121 ctcgccgatc cgggaccgag ctgccggagg cgccaggcgc gcacgctgtt ggcgccgccg
     3181 ctgtagaagc gccggtcgaa cgggatcagg tccgcctcgc ccgtcggatg ggccacgccc
     3241 acgaaggcct tccaggccag gacggagccg gggtccagcc ggatgtagcg gcgaaagtcg
     3301 cccaccaggc gcacgtactg tcggtagagg agccgccccg agcttccgcg accgcggaag
     3361 aacggcaggc cggggaggct gccctcgagc tctccgggcg tgagtacgta ccggtccagg
     3421 aggtacggca ggttgccgcc cagctcgacc gaaccctcgt agctgtatcc atcgctccgg
     3481 cggaacgggt cgacgcgcgc cgagcgcagg gtgtaccgca gcgcgttgtt gatctgcggc
     3541 tcggtgtaat cctccacgat ctggccgcgc tggatcgggt cgtcgatcgc ggcgagcacg
     3601 tcgtccagga agatctcgga gaagcccgcc agcgtgtccg ggctggacac ggtcaggtcc
     3661 aggagatcga ccagcgaagt gagcgtcgga ttgtggcgca tctcgagccg gtaccgggca
     3721 cctccccgcc cccggagcac cagccggagt gcgtcgcgcc gcgcggcgag gagcgagacc
     3781 gaaagctgcg tccgcgcatc gtacaggccg agccaccgtt cgaggcgggc gaacgggaag
     3841 gtcaggtacg ggtacgtgag cgacgccgag acctcccact gcgccgacgt gaagccgccg
     3901 gggccaccca ggtccgccgc gaccgttccg gtggactgca gcctgaactg ctcgccttcg
     3961 ccgaaggtgt tcagattgct gtaggtggtt ccgaggccca tgccgatctc gttgtccgag
     4021 tccgcgaggg ccccggatcg ctgcagcatg aacgtctgga ggcggatctg gtgacgcggg
     4081 cgggtctgca ggcgaaaggt gaggggtatc cggggatcgc cgcccgggcg cgcccgggtc
     4141 gtgtccgcca gatcggtctg gaagtcggtg aaggcgaaca cgcccgtggc atcgagtcgg
     4201 cgcttcgtgg cctcgagccg ctcctggtcg taccactcgc ccggccggaa ctgcagggtc
     4261 cgcatcagga ggcccgagga catgcggcgc tcgcgcgtga acgaggccgt gatccggccg
     4321 ccagggatgg aatcggccgg cgcggcggtc cggatcgtgt cggcgcgggg ttccggtgac
     4381 gcctcgatcc cggtgaccga ataggccacg tttccgaacc ggtaccgcat ccccggctgg
     4441 atatcgagga tcacgtcgaa cgaatcgggg cgggccggca cgatcacggc gtggatggaa
     4501 tcgcgggtga ccgcggcgta acccgccctt ctcaggaact ccaggatccg gcggccctcc
     4561 tcgagcagca gcggctccga gtaccgctgg ccgaccgccc gcctggtagt cgtgcggacg
     4621 gagtcgggta cgggggcgag cgcgaccagg gagccttcga ccaggcggtc gcgatgctcg
     4681 tccgagaggg ccccgacgcc ccggaacgac aggtcacgga tccacgtggg cgcgccgggc
     4741 tccaccacga agcgcacgcg ggcccggtcg ccccgctccg tgctgtcgat ccgggcggca
     4801 acgaaggcgg accggaagcc ctcctgcacg tagaagagcc tcagccgctc gacgtccgcg
     4861 tcgaccaccc gctggtccaa tatcgccggg ggctcgccgc tggaccgtat gccgcggccg
     4921 accgatcccc cgatcgaatc gcccaggcgg tacatccaga gccaccaggt cgccccggga
     4981 atgcccagaa ggtgccggtt ggcccgggtc ctgacgttca ggcggagctg ttcctcggga
     5041 aagaccaggc gaccctcgaa ctccacgtcg gagatcctga cggtccgctc gccgccgatg
     5101 ggagtctgtg cggaggcccg tccggcaggg gccgccagca ccgtcaggat gcacagtacc
     5161 gggaggaggg gacggaatga gggcatccgg gtgggtctat gtccggctcc ccgcccggac
     5221 cgcttcgttc cgggcccgga gtgcgatccg cacggtgtcc ggacaacggg cgcctactcg
     5281 tcctcgtagt agaagtcgtc gtcggcggtc ggaaaatccg gccagacctc ctcgatgctc
     5341 tcgtacggct cgccgtcgtc ttccatggcc cgaagattct cgatcacctc ggacggtgcc
     5401 ccggtccgct ccgcatagtc gatgagctcg agtcgggtcg ccggccacgg cgcgtcttcg
     5461 aggtgggagg caagttccag ggtccagtac atcacgattc ggcgcgttag gcgggcgctg
     5521 ccgggacgat agtttcttgg ttccgaaaaa gcaagccgat gaaaaaggcc acaaaacggc
     5581 cgttttcgcg gcccgaacgt actccgcgga aggaagtccg ttccgcgccc ctcaatcgaa
     5641 cgagatggtg ccctgctcgg actgccggag cagcttctgg taggcgaccg gcgtcatgct
     5701 cggggcgaag aaatagacgg gattcagcgc cctgcccgtg aggtcgcgca cctcgtaatg
     5761 caggtgcggc gccttgctca ggccggaatt gccggaaaac gcgatgagtt cgccacgcgc
     5821 cacggggcgg cccgccctga ggccgtcggc gaacctggac aggtgcgcgt acagcgtgac
     5881 gtacccggcg tcgcggtgct cgatcttcac gtaattgccg aatccgttgc ctcgtcccac
     5941 ctcctgcac
//
LOCUS       DBNHQZ010000384        19200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3229670, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000384 DBNHQZ010000000
VERSION     DBNHQZ010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3229670"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1537)
                     /locus_tag="AC16YU_10395"
     CDS             complement(287..1537)
                     /locus_tag="AC16YU_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR6065679.1"
                     /translation="MPIKALMLAAGRGSRLGPQTSDLPKPLLQIGTGHLVEHQLSTLA
                     DAGIGPVGMVLGYHADDIRDVVGMRAEYLYNGRWATTNSMASFLAAKEWIDDDLIVLN
                     CDVLFHGDVLRRLLAAGPDSFAFDSTSGEGLEQMKVQLDPEGRLLRMSKTLDAGDTSG
                     ENVGILYFGKSTARRLVEIAERRIEQGHTKDWLGSAVQELCTERPLKGVDVAGCPWVE
                     IDFAYDLDRARKEVYPSLAPKPRKWPRRLGYAAAAMLMVAVTGLVAGLAAVQGHGSTG
                     SESTVLMEVIPLSGGTKTTLANGSAHQTWWLVDNGESVSADIEGPAQFQVESRLVLDR
                     DSSAASPYLLEITVDRDVIDWIRPDLERSRSIRFEGGRVGKRSRETIEIGPGSHRLRI
                     LATLVAERRVLLRLRQVEPDFADD"
     gene            complement(1563..3275)
                     /locus_tag="AC16YU_10400"
     CDS             complement(1563..3275)
                     /locus_tag="AC16YU_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6065680.1"
                     /translation="MKGLTSRYRNTVRTLRDYGRPHRGVLVWGMVGTIAVVALRLAFP
                     WPLKWVMELAIDAAAGSAHGHVFIPLLGNAGLSAVLGLFIALGALLGLAEMAQRVYLK
                     RYAAMTVRDIRDRASRNVSSTGRRDGEIIARMVGDAARVKAELSGILVHVSQNFLLFA
                     GVCLVFVGIAPYLALFFLTSGLFAIVVGYRSTRRVESLSLKQRSKEADYAVVLADAVQ
                     RGTLESGASSLNRQSTRQDVMVTRTIAGASALVHTGVAVFTAVALALSVPGVAAGWLD
                     PGDLFLFIAYALTVHRRLVQVGRQLARSGKLTANLNRLAELAGPDEAGAVATPSLHEA
                     IGLHEVRVGGGRGWTGRRRLSRTSLTIPRGSRVLVVGGTGSGKSTLLRVLAGRDAHKG
                     VVLWDGVPVTGRAVRESPLVKWVPQAPVFGRSRLGTLLGLDDADGHGDQAAVLHASGI
                     PHLIARAPKGLATKVSSTSLTLVEAYGIALSRLLIEQTGDVWLIDEPFAGLSSKRSSI
                     RLGTVLAAAAGRTLVIASVRAVLPDAFDLVVAMKSGRVRFSGTADEYRNWMIRGRTES
                     PVAP"
     gene            complement(3288..4415)
                     /locus_tag="AC16YU_10405"
     CDS             complement(3288..4415)
                     /locus_tag="AC16YU_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIR6065681.1"
                     /translation="MPADVLFTGYAPVHFLCFRPVYERLKKDTDIRVFLSGGYRHKRA
                     SGVSYDLKGFFDFPGVAREDVIPLDEARQRDFDVLVNSHLSDSLFPRSAARTVQIFHG
                     VSYKNYMARDKALRFDTLCLPGPYHAGLYRERGLIRNDGPEVLLTGFAKDDPLVDGSL
                     DRDALLERHDLDPHLPSVLFAPTGDRHNSLELYGEAVIEHLSRGGAWNLLIKPHDHPK
                     DPIGWFERLAPLETDRVRLVRDLDVVPWLHAADLLLSDASSVITEFTLLDRPIVLLHV
                     PKLLERVVRRGGALDTTSYVSQLAPVVDDPADLPSIVASSLASPTAASEHRVRMARDV
                     FYRPGTAAERVHGVVRYAAGLASLPADVVRLEDGGSRVWAA"
     gene            complement(4418..5560)
                     /locus_tag="AC16YU_10410"
     CDS             complement(4418..5560)
                     /locus_tag="AC16YU_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065682.1"
                     /translation="MRTPTSLRRLLAHILLLHGLASESIATPPNVGGLRPDRTADPVW
                     VADTLAIPRIGNRVKAASGPERRLVISMSTEMDSNPFRMKDRWRSDFEGMRDVGERFA
                     GYESVSDQAVEATIAGSLTWKPRGLREVGFDAGLAYQTLLRNGFADQLDVEAGFWVSA
                     SKGARTSFNLDWTPSHFRANYVNESASVSGFEPAHASDIGLRLRHRHRLSGAVDLVGD
                     AAVEWRNYEDAFAERDRRVLAGGLQVKLEPARRLRLEIGLSASRGTTTNGGARFDRSF
                     GQIGGEAGISSRIGDWRPSLTIGVRKRPYTTADESDQTRFGRVDSSWELGIAATRPVA
                     RGIDLFIGARYREKKSNRPGDPLDPDVVPYTRYSVRLGFTATLKGG"
     gene            5813..8062
                     /locus_tag="AC16YU_10415"
     CDS             5813..8062
                     /locus_tag="AC16YU_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6065683.1"
                     /translation="MEGRTVSRFRILNRLGSGGMGVVYRAEDTLLGRHVALKFLPKEV
                     ARSVEAKQRFMVEARAASALQHRGICTIHDIGETDEGELYICMELYLGETLRQRLSRG
                     PLPVPDAMRIAAQIAGAMAKAHSGGMVHRDIKPENVIFTDDDDAVILDFGLAKLASES
                     GLTRPDASVGTVGYMAPEQAGGHAVDQRADLFSLGVMLYEMVAGVHPFAAGHPVAVLY
                     RAMNEEHEPLSAVRPDISPALGELVDRLLRKDPTRREASAAGVSAALGALMAAPASSA
                     PHPPTAGAHRGSATIESLAVLPFLNQSEDPEVDYLSDGITDSLIDTLSRLHRLRVMAR
                     STVFQYGSRAVNPVDVGRELGVRAVLTGRLSLRGDMLLIRAELVDATDGTSLWGERYR
                     RPVADLMAIEEEISRQISRSLLVRLDPDEESRLSGRGAVDPGAYQAYLKGRRVWNQWK
                     TADGMRSAIGFFEQALRLDPLFAPAFAGLADSYSILGNIKAIPPQEAFPKAKGYARQG
                     LAISDEVGELHSSLAFIQRHWDWDWNAADASYRRAIELNPGYATAYRFYGGLLTGLGR
                     HEEGIERARKALDLDPLSLIIRTALGDAYFYARRYDESIAMYEDSIRMDEGFLAGHSD
                     LARSLEITGRYDDAIREYRTAAALAAKVPPEPSSGLANVYARMGRRDDAVRILDELIA
                     MRGRSYVSPYGIASIYASLREVDSAFEWLDRAYEEHDQTMVLVKVHPRLDPLRTDARW
                     SGILERMRL"
     gene            8085..9059
                     /locus_tag="AC16YU_10420"
     CDS             8085..9059
                     /locus_tag="AC16YU_10420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6065684.1"
                     /translation="MKRKILLAMAGLAIVVVAVPVIAVGPYALRVDRFEADPGRGYHA
                     AFYLYVPPGARRATEAGDRVTLLVQPNNSGTNSDDPKVHEKDAWWTGFGRHGLADELN
                     VVLLVPAFLRPGEDWRIYTHALDRDVLTTSRGDLARLDLQLLAMVDRARSELAGDGMA
                     TGERILIQGFSASGMFANRFAVLHPDRVLAVAAGSPGGWPIAPLASWEGETLPYPAGT
                     ADLEELTGQPFDSLGYARVPQLIVMGSLDDNDSVDFRDGWDEEPAALVNRLFGADPIA
                     RWGIAETVYRAAGANARFILEEGVGHDRRRLQHHTTRFFADVLAGQAP"
     gene            9154..11307
                     /locus_tag="AC16YU_10425"
     CDS             9154..11307
                     /locus_tag="AC16YU_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6065685.1"
                     /translation="MSENHPNHSDHDGRGAADHGEHAEHGQEHAGHEKHAAPEKHAAP
                     GKHAAPGKHAGHDAHAGHDKHEGHSPEMFRSKFWLSLLLTLPVVYWSAHVQELLGFTA
                     PEFPGSAWIPPILGTVVFVYGGWVFVKGALRELRARLPGMMTLIALAIAVAFVFSWVV
                     QVGLIRAEARWWELATLVTLMLLGHWIEMRSVNQAQGALQALAKLLPDTATRVTDAGE
                     EEVAVDELRDGDLVLVRPGEGVPADGVVRRGESDVNESMITGESRPVKKGEGDEVIAG
                     TINGAGSLRVEVTGTGENTKLSGIMRLVADAQQSKSRAQHLADRAAQILTVVALAAGS
                     ITFVVWQLTGAAVEFSVVRMVTVLVIACPHALGLAVPLVVAISTTLGAQAGLLVRDRR
                     GLEEARNMDTVVFDKTGTLTLGEFRVVEMTVAEDLSEEDALRIAAGVESESEHPIARG
                     IVRTAEDRDLEIPASDGFRAITGKGVAARVDGAEYHMGGPALLEEEGADVPPGLLEAA
                     DAASDRGQAAIYLLRDGRPLAVFAVADAIREESREAIRALHDRGIEVAMLTGDARAVA
                     DAVAEDLGIDTVFAEVLPEDKASKIRELQEGGRRVAMVGDGVNDAPALATADIGIAIG
                     AGTDVAVEAGHIVLVRSDPRDVARIVTLSRATYRKMLQNLWWAAGYNIVAIPLAAGAL
                     AAWGILLTPAVGAVLMSASTVIVAVNAQLLKRTRL"
     gene            11417..12268
                     /locus_tag="AC16YU_10430"
     CDS             11417..12268
                     /locus_tag="AC16YU_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065686.1"
                     /translation="MARRPVLALLLLLPAVTAHAQVDDHFPLAVGNAWTYFTPLDSVP
                     PDTVWDHTSGVAAAVSVNDTLYFVVDYPFAPADTLRADGDGKVFARLGGRDLLLFDFT
                     VAGGETYPFASPSAPEVNFTVTVDRLDMCEAAAGRFPDCVRLAFDDPEWSDEEFTFVF
                     AQGVGIVWAYGDGGWYEELFAARVAGRIITSREWAASTAAQAFAYPNPFGDSATIVVP
                     ATGAATMRVRVYDVLGRLAATPGEGMCDGLTCRFRLDGALLPAGPYFVRAEQGGHVRT
                     MRLILRR"
     gene            12265..13587
                     /locus_tag="AC16YU_10435"
     CDS             12265..13587
                     /locus_tag="AC16YU_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6065687.1"
                     /translation="MTRPDRRAAPGSIRESGSTVRLARVVGIPGAVLMGLGSILGTGI
                     FVSIGIAAGVAGPSVVLAVALAAVVAAFNGLSSAQLAAGHPVSGGTYEYGYRWLTPTL
                     GFTAGWMFLAAKSASAATAALGLSGYVLTALGLPGAGPRIALAIGSVAVLTMIVASGM
                     SRSNRANAVIVSVTLAALAAFVVAGLPSAADGAALNVSGLFDAGGLQRLLHATALMFV
                     AYTGYGRIATLGEEIRDPARSIPRAIIVTLLVTMGLYVSVTLVAVAAVGAPAMAEATG
                     VAAAPLEVIARGFGVPGVGWIVAVGAVTAMLGVLLNLLLGLSRVLLAMARRGDMPRGL
                     ARVEGIRASPRRAVFAVGAVIALLALTGSVRTTWSFSAFTVLVYYAITNLAALRLPAD
                     LRLYPRWVAAAGAASCLGLAFWVEPRIWLAGLGLIAVGLVWRGVARRP"
     gene            complement(13594..14040)
                     /locus_tag="AC16YU_10440"
     CDS             complement(13594..14040)
                     /locus_tag="AC16YU_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6065688.1"
                     /translation="MRRHVHEEVIPAPPDAVFRLLHTPSAICAWWGARTAVVLAVEAG
                     YWTASWGESDDDPDYVSAARLVEFDPPRRLAFGDSRYFARSGPLPFEASFVTTFEVEP
                     HPDGSILRVTQDGFPDAAVADDFYAACDTGWRDTFAGIRRYLEAGR"
     gene            14100..14855
                     /gene="hisF"
                     /locus_tag="AC16YU_10445"
     CDS             14100..14855
                     /gene="hisF"
                     /locus_tag="AC16YU_10445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR6065689.1"
                     /translation="MLTKRIIPCLDIKDGRTVKGVNFVGLRDAGDPVELGARYAREGA
                     DELVFLDITATLEDRDTFVALVRDIASHLDIPFTVGGGISQVSHVAALLEAGADKVSI
                     NSAPVRRPALVSELAAEFGSQCVVLAVDTRRLGGRDLVHTHGGTRPTEIETGVWIREA
                     VDRGAGEILLTSMDHDGTKEGFAVDITRAVSQDVPVPVIASGGAGAREHFLAVFRDGL
                     ADAALAASVFHFGEIPVPELKAYLHENGVPVRI"
     gene            14859..15464
                     /gene="hisIE"
                     /locus_tag="AC16YU_10450"
     CDS             14859..15464
                     /gene="hisIE"
                     /locus_tag="AC16YU_10450"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR6065690.1"
                     /translation="MDIDFEKGGGLVPAIVQDARTGTVLMLGYMSREALDRTVQTGLV
                     TFFSRERGVLWTKGETSGNTLAFESMAVDCDGDTLLVRARPSGPVCHTGTDTCWGESN
                     EPDPMAFLSELDAVVAERSGAPPEESYTRRLLDGGAAAAGQKVGEEAVETVVAALAQD
                     DGRVVDEAADLVYHLVVLLRARGLSLGDVALRLEERHRPGR"
     gene            15583..17874
                     /locus_tag="AC16YU_10455"
     CDS             15583..17874
                     /locus_tag="AC16YU_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6065691.1"
                     /translation="MPHSCRLALVLAALLLPGPALAQTGTVRGFVTEARSEGPLQGAN
                     VSLLVPGGETRGTVTDGDGYFRIPAVPAGEQRIVVTYIGFRPYDETVRVPDGGTITLR
                     IALEESAEVMEEVVIEAEGGSGVAAVTAGLQAIRPRDVARVPIHGASGDLAAYVQSVP
                     GVVLPGDRGGRFHIRGGSDDQNLVLVEGIPVYGAFHILSLYSAFPDEVIDQADFYTAG
                     FGARYGERLSSVLDVRTRNGNKQAFGGSASLSPFLSGVRLEGPIIPGRASVLLSGRRS
                     LVGDLMPSVLGQNMPYRFGDGFGKLHAFLGSGHSLSVFGLLSDDEGQIAGTQYTFDGE
                     LVQTPATDSLDLRWDNRVVGGTYRLEPGWGQPVRAEFTVGHSRWRSYFGPTGEEARQT
                     GLQSLDIRLDLGFVVSGVDVRLGTLRRSTDVTYHLDDPFREAPEAYDRNRVERSDYAQ
                     LDIPLRALGLWIQPGVRAYGVGEEPVLWEPRVRSTLELPGFDGTHRLTAAWGRMHQSL
                     VGLVDERDVGNPFAVWTVSEADRPVPLASHAVVGWNAAPLRDIQVAVEVFEKRFEHLQ
                     VPVFSAFPTFTTALQTADGRARGVDVRVSFENVPGPYESRVGGWVSYALAEVEYETAN
                     GTEYAPAHDRRHSVQAVVDATKDDMALLAQVQVGSGLPYTPSSGFDTWYLMTPGVDVA
                     SDPGRQRVLYARQNSERLPLYARLDLWIERRIDRGRSVITLRAGGTNVFNRANLFYFD
                     LFTYRRVDQLPFMPSVGVRIEFR"
     gene            17871..18797
                     /locus_tag="AC16YU_10460"
     CDS             17871..18797
                     /locus_tag="AC16YU_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HIR6065692.1"
                     /translation="MIRRLLFLVAGLAALAAGCDDPFIDPFANEDRWFTVWGWIDPDA
                     ARQEVRVIPVTRRPSTIASPDDMNAAVNARVFSIDLGSGTETEWAYRLAQLDDGTYGH
                     VFSASLFVRPADSLRLEVRRSDGRVTWAETVVPTPTPISLGDIGVVYEAENGHLAQDI
                     VVRGVERPWSLRVTYRVEGSLIDKWFKTVDYGRSGRSDGQGNWIFTVDLTADQEAIRE
                     DIRAAVEAGAVPRPEFGDIPDAGLNRMNVGVLELDGTWDLPFGEYDDEAYAFPDALTN
                     VVNGYGYWGSGSRVSQQWEVDSILSRRLGWPF"
     gene            complement(18807..>19200)
                     /locus_tag="AC16YU_10465"
     CDS             complement(18807..>19200)
                     /locus_tag="AC16YU_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6065693.1"
                     /translation="HPGTTFINAHLGWFGSDLDRLGAMLDALPNVVTEVGAVVQELGR
                     QPRRAREFLVRYHDRVLFGKDTYRPFEYHTYFRTFETADEYFPYYRKYHAFWRLYGLD
                     LPDDVLRDIYYRNALRIIPGLDPSLFPE"
BASE COUNT         2932 a         6482 c         6734 g         3052 t
ORIGIN      
        1 tcgcaaggcg taccgagggt tcgaatccct ccctctccgc acggacgccc gcctgcgcac
       61 gcgcagcggg cgttcggcgt ttcggggggt ccccgttcga gcttgctcta aaaggggagg
      121 ccccgaaacg ccgaaacccc atcggggtga acaggcggac gtccgtgcag gacctccccc
      181 ccgggatccc cagcgagcgc agcgagcgga atcccgaccg tgacaggcgg acgtccgtgc
      241 aggacctccc ccccgggatc cccagcgagc ggaatccctc gcgccctcaa tcgtcggcga
      301 aatccggctc gacctgccga aggcgaagga gcacccgcct ctcggccacg agggtagcga
      361 gaatccggag acggtgtgaa cctggtccaa tctcgatcgt ctcgcggctt cgcttgccga
      421 cacgaccgcc ctcgaatcgg atcgaccggc ttcgctccag atcgggtcga atccagtcga
      481 tgacgtcacg gtcgaccgtg atctcgagca ggtacggact tgccgccgac gagtcacggt
      541 ccaggaccag gcgggattcc acctggaact gcgccggccc ctcgatgtcg gccgagaccg
      601 actctccatt gtcgacgagc caccaggtct ggtgagcgga tccgttggcc agggtggtct
      661 tcgtcccccc gctcaggggg atcacttcca tgagtaccgt ggactcgctg ccggtcgatc
      721 cgtgcccttg cacggccgcc aacccggcca ccaaccccgt cactgctacc atgagcattg
      781 ccgcagccgc gtatccgagc cggcgcggcc acttccgagg cttgggtgca agggagggat
      841 acacctcctt tcttgcccgg tcgagatcgt acgcaaagtc gatctccacc cacggacatc
      901 ccgccacgtc cacgcccttc aagggtcgct ccgtgcacag ttcctgcacg gcagagccga
      961 gccagtcctt tgtgtgaccc tgctcgatcc gacgctccgc gatctcgaca agtcgacggg
     1021 cagtcgactt tccgaagtac aggatgccca cgttctcgcc gctcgtatcg cccgcgtcca
     1081 gcgtcttgct catcctgagc aggcggccct ccggatcgag ctgcactttc atctgctcaa
     1141 gaccctcgcc ggacgtggaa tcgaatgcga aggaatcggg gccagcagcg agcagtcgcc
     1201 tgaggacgtc cccgtggaac aggacgtcgc agttgagcac gatcaagtcg tcgtcgatcc
     1261 actccttcgc tgcgaggaac gaggccatac tgttcgtcgt ggcccagcgg ccgttgtaca
     1321 ggtactctgc ccgcataccg accacatccc ggatgtcgtc cgcatggtac ccgagcacca
     1381 ttcccacggg gccgattccg gcgtcggcca gggtcgacag ctggtgctcg accagatgcc
     1441 ccgtccctat ctgcagcagc ggcttcggca gatcggaagt ctgtggtccc aaccggcttc
     1501 cacgtccggc ggcgagcatg agcgctttga taggcatggc aggagacggt tacatgacgc
     1561 cctcagggcg cgacgggtga ttccgtccgg ccgcggatca tccagttccg gtattcgtcc
     1621 gccgtccctg aaaaccggac ccttcccgac ttcatggcga cgacgaggtc gaacgcatca
     1681 ggaaggacgg ctcggaccga ggcgatcacg agagtccttc cggccgctgc ggcaagaacc
     1741 gtcccgagac ggatggacga ccgtttggac gaaaggccgg cgaagggttc gtcgatgagc
     1801 caaacgtcgc ccgtctgctc gatcaggagg cgactcagcg cgattccgta cgcttcgaca
     1861 agggtgaggg aggtcgagga gaccttcgtc gcgagaccct tcggcgcacg ggcgatgagg
     1921 tgcgggatgc cgctggcgtg gaggaccgcc gcctggtcgc cgtggccatc cgcatcgtcc
     1981 agcccgagga gcgtccccag tcgggatcga ccgaagacag gcgcctgggg tacccacttc
     2041 accaggggcg actcgcgcac cgcgcggccc gtcaccggaa ctccgtccca gaggaccaca
     2101 cccttgtgcg cgtccctccc ggcgagcact ctcagaagag tggactttcc tgatcctgtg
     2161 ccgccgacca caagcaccct cgagccgcgt gggatcgtga ggctcgtacg ggaaaggcgc
     2221 cgccgccccg tccagccccg acctcctccc acccggactt cgtgcaatcc aatcgcctcg
     2281 tgcagggaag gcgtcgcaac tgcgccggcc tcgtcgggac ctgcgagctc tgcgagacgg
     2341 ttcaggttgg cagtgagctt gccactgcgg gcgagctgcc ggcctacctg gaccagccgc
     2401 ctgtgcaccg tcagggcgta cgcaatgaac aggaagaggt cgccggggtc gagccacccc
     2461 gccgcgacgc ccgggaccga caatgcgagc gcgacggcgg tgaagaccgc cacgccggtg
     2521 tggaccaggg cgcttgcccc ggcgatggtc cgcgtgacca tgacgtcctg ccgggtgctc
     2581 tgccggttca gggaactggc tccggattcc agtgtgcctc tctggacggc gtcggcgagg
     2641 acgacggcgt agtcggcttc cttggaccgc tgcttcaggg agagggactc cacccgccgg
     2701 gtcgatcggt atccgacgac gatcgcgaag agcccgcttg tgagaaagaa gagggccaag
     2761 tacggagcga tcccgacgaa gacgagacag actccggcga agagaaggaa attctgtgag
     2821 acgtggacca gaatgcccga cagttccgcc ttgacccgcg ccgcgtctcc caccatgcgg
     2881 gcgatgatct ctccgtcgcg gcggccggtc gaagaaacgt ttcgtgacgc cctgtcgcgg
     2941 atatcgcgga cggtcatggc ggcgtaccgc ttcaggtaca cgcgctgtgc catttccgcc
     3001 agaccgagca gggcaccgag ggcgatgaac agcccgagga cggccgacag acccgcgttg
     3061 cccagcaggg ggatgaacac atgtccgtga gccgaacccg cggccgcgtc gatcgccagc
     3121 tccatcaccc atttgagggg ccagggaaag gcgagccgca gtgcgaccac ggcgatcgtc
     3181 ccgaccatgc cccagacgag gacgccccgg tgggggcggc cgtaatcccg aagggtgcgg
     3241 acggtgttcc gatatcggga ggtgagtcct ttcatgcgaa ggacgtttca ggccgcccac
     3301 actcgactgc caccatcctc caggcggacg acgtcggcgg ggagggaggc cagaccggcg
     3361 gcgtagcgga ctacgccgtg aaccctttcg gcagccgtgc ccggcctgta gaagacgtcg
     3421 cgcgccatgc gcaccctgtg ctcggacgcg gccgtcggac tggccaggga cgaggccacg
     3481 atcgacggga gatcggccgg atcgtcgacg accggggcca actgactcac gtaggacgtc
     3541 gtgtcgagcg cgcccccccg ccgcacaacc cgctcgagga gtttcggaac gtggaggagc
     3601 acaatgggcc ggtcgagcag ggtgaactcg gtgatcaccg aggaggcgtc ggacaggagc
     3661 aggtcggccg cgtgcagcca cgggacgacg tccaggtccc gaacgagccg cacccggtcc
     3721 gtttcgagag gagcgaggcg ctcgaaccag ccgatcggat ccttggggtg gtcgtgcggc
     3781 ttgatcagga ggttccaggc tccaccccgt gacagatgct cgatgaccgc ctccccgtac
     3841 agctccaggg aattgtggcg gtcccccgtc ggtgcgaaca gaacgctggg cagatgcggg
     3901 tccagatcgt gccgctccag cagggcatct cggtcgaggc tcccgtccac gagcgggtcg
     3961 tccttcgcga agcccgtgag cagcacctcg ggaccgtcgt tccggatcag gccgcgttcg
     4021 cgatacagac ctgcatggta tggcccggga aggcagaggg tgtcgaaccg gagggccttg
     4081 tcgcgggcca tgtagttctt gtacgacacc ccgtggaaga tctgcaccgt tcgggcggcg
     4141 ctcctgggga agagactgtc ggagagatgc gagttcacca ggacgtcgaa gtcccgctgt
     4201 ctcgcctcgt cgagcggtat gacgtcctcc cgggccaccc cgggaaagtc gaagaagcct
     4261 ttgaggtcat aggagacccc ggaggcgcgc ttgtgacggt agccgcccga taggaagacc
     4321 cggatgtcgg tgtctttctt caggcgttcg tagacgggcc tgaagcaaag gaagtgcacg
     4381 ggcgcgtatc cggtgaagag aacatctgcc ggcatggtca gccccccttc aacgtcgccg
     4441 tgaagcccag acgaacgctg tacctggtgt acggcacgac gtccggatcg agggggtcgc
     4501 ccggtcggtt ggacttcttc tccctgtatc tggctccgat gaacaggtcg atgccgcggg
     4561 ctacgggccg ggttgccgcg attccaagtt cccaggacga gtcgacccga ccgaatcgcg
     4621 tctggtccga ctcatccgcc gtggtatagg gccgtttccg gacccctatc gtaagggagg
     4681 gcctccagtc accgatccta ctggaaatcc ctgcttctcc cccgatctgg ccgaaggacc
     4741 ggtcgaagcg ggctcctccg ttcgtcgttg ttccgcggct ggcgctcagg ccgatctcca
     4801 gtcgcagcct tcgggccggc tccaacttca cctgcaggcc gccggcaagc acgcgccggt
     4861 cgcgttcggc gaaggcatcc tcgtagttgc gccactcgac cgccgcgtcc ccgacgagat
     4921 ccacggctcc cgacaggcga tgccggtgtc tcagacgcag tccgatgtcc gaggcgtgtg
     4981 ccggctcgaa accggacact gacgccgact cattgacgta gttggcacgg aagtgactcg
     5041 gagtccagtc caggttgaag gaagtgcggg ctcccttcga agcgctgacc cagaatcccg
     5101 cctcgacatc caactggtcc gcgaatccgt ttcgcaggag ggtctggtag gcgagccccg
     5161 cgtcgaatcc gacctcccgg aggcctcggg gcttccaggt cagacttccg gcgatggtag
     5221 cctcgacggc ctgatcggaa acggactcgt aacctgcgaa gcgctccccg acatcccgca
     5281 tgccttcgaa atcgctccgc cagcggtcct tcatacggaa tggattcgaa tccatctcgg
     5341 tgctcatgga gatgaccagt cgcctttccg gaccggacgc ggctttcact cgattcccga
     5401 tgcgcgggat cgccagcgtg tccgcgaccc acacaggatc ggcggtccga tccggacgga
     5461 ggcctcccac gttcgggggc gttgcgatgg actcgctcgc caacccgtgg agcagcagga
     5521 tgtgcgcgag gaggcgtcgt agcgaggtgg gggtgcgcac gtcgttgatc ggcgatccgt
     5581 atgtcccacg gtatcgggat tccgacacct gacttaagcg gcgggagcgg ccgcgccgcg
     5641 ggattcctgt aggtgaagac cagcccgctc cgtccccgct acgggccgtc tcatcgccct
     5701 gtctcggcgg gcgtcgtccg gtcccaggcg ggccacccgt cggccgcgtc gccgcgcgtg
     5761 accacgccgc cgattttcgt taccatgagc agcatcctga ccagctccag ccgtggaagg
     5821 ccgaaccgtc tcccgtttcc gcatcctgaa ccgactcggc tcgggcggaa tgggcgtggt
     5881 ctaccgggcg gaggacaccc tgctcggccg gcacgtcgca ctgaagttcc tgccgaagga
     5941 agtcgcccgg agcgtcgagg cgaagcagcg cttcatggtc gaggcaaggg ccgcctcggc
     6001 gctgcagcat cggggcatct gcacgatcca cgacataggc gagaccgacg agggggaact
     6061 gtacatctgc atggagctct acctcggaga aacgctcaga cagcggctct cgcgcggccc
     6121 actgcccgta ccggacgcga tgcgcatcgc tgcccagata gccggcgcca tggccaaggc
     6181 ccactcgggc ggcatggtgc atcgtgacat caagccggag aacgtgatct tcacggatga
     6241 cgacgacgcg gtgatcctgg atttcggtct cgcgaagctg gcgagcgaat ccgggctcac
     6301 acggccggac gcgtccgttg gaacagtggg gtacatggcg cctgaacagg ccggcggtca
     6361 cgcggtagac cagcgggccg acctcttctc actcggggtg atgctctacg agatggtggc
     6421 cggcgtccac ccgttcgccg cgggacaccc tgtagcggtg ctctaccggg cgatgaacga
     6481 ggagcacgag ccgctctcgg ccgtccgtcc ggacatctcc ccggcgctcg gcgagctggt
     6541 ggaccgactg ctccgaaagg acccgacccg gagggaggcg tcggccgcgg gcgtgtcggc
     6601 ggcactcggt gccctcatgg cggcgcccgc gtcctcggcg ccccatcccc cgactgccgg
     6661 ggcacatcgg ggaagcgcga ccatcgagtc gttggctgtc cttccgttcc tgaaccagag
     6721 cgaagacccc gaggtcgatt acctgagcga cgggatcacc gactcgctca tcgacacgct
     6781 gagccgcctg catcgcctgc gcgtcatggc acgcagcacg gtgttccagt acggatcgcg
     6841 ggccgtgaat ccggtcgacg tcggtcgcga actgggcgtc cgcgccgtgc tcacggggcg
     6901 cctgtcgctg cgcggtgaca tgctgctgat ccgagccgag ctggtcgatg ccaccgacgg
     6961 caccagccta tggggggagc ggtaccgccg gcccgtcgcc gacctcatgg ccatcgagga
     7021 ggagatctcg cgacagatat cccgaagcct gctcgtgcgg ctggatcccg acgaggagtc
     7081 gcgcctctcg ggccgcggcg cggtcgatcc gggggcgtac caggcgtacc tgaaggggcg
     7141 tcgcgtctgg aaccagtgga agaccgccga cggcatgcga agcgccatcg ggttcttcga
     7201 gcaggcgctt cggctcgatc ccctgttcgc ccccgcgttc gcggggcttg ccgacagcta
     7261 ctccatactg ggcaacatca aggcgattcc gccgcaggag gccttcccca aggcgaaagg
     7321 atacgcgcgg caggggctgg ccatcagcga cgaggtgggc gagctgcatt cgtccctggc
     7381 attcatccag cgccactggg actgggactg gaatgcggcg gatgcgtcgt accgccgcgc
     7441 catcgagctg aatccgggct atgcgacggc gtacaggttc tacgggggcc tgctgaccgg
     7501 cctgggccgt cacgaggagg gtatcgagcg ggcccgcaag gccctggacc tggatcctct
     7561 gtcgctcatc atccggaccg ccctgggcga cgcctacttc tacgcccgga ggtacgacga
     7621 gtcgatcgcg atgtacgagg actcgatccg tatggacgag ggcttcctcg cgggtcacag
     7681 cgatctcgcg cgttccctcg agatcacggg gcgatacgac gacgcgatcc gcgaataccg
     7741 gaccgccgcg gcgctggccg cgaaggtgcc gccggagccc tcgtccggac tcgcgaacgt
     7801 gtacgcgcgg atgggccgcc gcgacgacgc cgtgcggatc ctggacgagc tcatcgcgat
     7861 gcgcgggcgg agctacgtgt ccccgtacgg gatcgcgtcc atctacgcgt ccctccgcga
     7921 agtggactcg gcgttcgagt ggctggatcg cgcctacgaa gaacacgacc agaccatggt
     7981 cctggtgaag gtgcatccca ggctggatcc gctccgtacc gacgcccggt ggagcggaat
     8041 actcgaacgg atgcggctgt gaacgcagcc gctggactgc gcccatgaag aggaagattc
     8101 tcctggcaat ggcggggctc gccatcgtcg tggttgcggt tcccgtgatc gcggtcggtc
     8161 cctacgcgct gcgcgtcgat cgcttcgagg cggatccggg gcgaggctac cacgcggcct
     8221 tctacctgta cgtcccgccc ggggcgaggc gtgccacgga ggcgggcgat cgcgtgacac
     8281 tgctggtgca gccgaacaac tcgggcacga attccgacga cccgaaggtc cacgagaagg
     8341 acgcctggtg gaccgggttc gggcggcacg gcctcgcgga cgagctgaac gtcgtgctgc
     8401 tcgtgccggc cttcctccgg cccggggagg actggcgcat ctacacccac gccctcgacc
     8461 gcgacgtcct gacgacttcg cgcggcgacc tcgcgcggct ggacctgcag ctcctggcga
     8521 tggtggaccg ggcccggagc gaactcgccg gtgacggaat ggccaccggg gaacgcatcc
     8581 tgatccaggg attctcggcg agcggaatgt tcgccaaccg cttcgccgtg ctccatccgg
     8641 accgcgtact cgccgtcgcg gcggggtcac ccggaggatg gccgatagcg cccctggcgt
     8701 cgtgggaggg cgagacgctg ccgtaccccg ccggtacggc cgacctggag gaactgacgg
     8761 ggcagccctt cgactcgctc ggctatgccc gggtgcccca gctgatcgtc atgggatcgc
     8821 tcgacgacaa cgattcggtc gacttccggg acggctggga cgaggaaccc gcggcgctcg
     8881 tgaatcggct cttcggcgcc gatccgattg cccggtgggg catcgccgaa acggtctacc
     8941 gcgcggccgg agccaacgcg cgcttcatcc ttgaggaggg cgtcggacac gaccgcaggc
     9001 gcctgcagca ccacacgacc cggttctttg cggacgtgtt ggctggccag gcgccgtgac
     9061 gaacggccca ggcgggtcga atgcgggtgg cgtacgcccg tagaaacccg tgcgtccgat
     9121 tcgtttattc ggagaacccc acggagcgca cccatgagcg agaaccaccc gaatcactcc
     9181 gatcacgacg gacgcggtgc cgccgaccac ggcgaacacg cggagcacgg gcaggagcat
     9241 gccggccacg aaaagcacgc cgcccctgaa aagcacgccg cccccggaaa gcacgccgcc
     9301 cccggaaagc acgcgggcca cgacgcgcac gccggccatg acaagcacga gggccacagc
     9361 ccggagatgt tccggagcaa gttctggctc tcgcttctcc tcacgctgcc cgtcgtctac
     9421 tggtccgcgc acgtccagga actcctcggc ttcacggcgc cggagtttcc cggctcggcg
     9481 tggattccgc cgatcctggg cacggtcgtg ttcgtctacg gcggatgggt cttcgtgaag
     9541 ggagccctgc gcgagctgcg cgcgcgcctg cccggaatga tgaccctcat cgcgctggcc
     9601 atcgcggtcg cgttcgtctt ctcgtgggtc gtgcaggtgg gcctgatccg ggccgaggcg
     9661 cgatggtggg agctcgcgac cctggtgacc ctcatgctcc tgggtcactg gatcgagatg
     9721 cgttcggtga accaggccca gggggcgctg caggcgctcg cgaagcttct ccccgacacg
     9781 gcgacgcgcg tgaccgacgc gggggaggag gaggtcgcgg tcgacgagct gcgagacggc
     9841 gacctggtgc tcgtccgtcc gggtgagggc gttcccgcgg acggcgtggt gcgtcgcggg
     9901 gagagcgacg tcaacgagtc gatgatcacc ggggagtcca ggccggtgaa gaagggggaa
     9961 ggggacgagg tgatcgccgg taccatcaac ggggcgggat ccctccgggt ggaggtgacg
    10021 ggcacgggag agaacacgaa gctgtcgggg atcatgcgcc tggtcgccga tgcccagcag
    10081 tccaagtccc gggcccagca cctcgcggac cgggccgcac agatcctgac cgttgtggcg
    10141 ctggcggccg gcagcatcac gttcgtcgtc tggcagctga cgggggcggc cgtcgaattc
    10201 tcggtggtgc gcatggtcac ggtgctggtc atcgcctgcc cccacgccct gggtctcgcc
    10261 gtgccgctcg tggtggccat ctccaccacg ctcggggcgc aggccggtct gctggtccgg
    10321 gatcgccggg gtctcgagga agcccggaac atggataccg tggtcttcga caagacgggg
    10381 accctgacgc tcggcgagtt ccgggtcgtc gagatgaccg ttgcggagga cctctccgag
    10441 gaggatgcgc tgcgcatcgc ggccggcgtc gagtcggagt ccgagcatcc cattgcgcgg
    10501 ggcatcgtca ggacggctga ggaccgggac ctggagattc cggcctccga tggcttccgg
    10561 gcgatcaccg gcaagggagt cgccgcccgg gtagacggcg cggagtacca catgggcggt
    10621 cccgcgctgc tcgaggagga gggagcggac gtgcccccgg gcctcctgga ggcggccgat
    10681 gccgcatccg atcgggggca ggcggccatc tacctgctca gggacggacg gccgctcgcg
    10741 gtcttcgccg tcgcggatgc gatccgggag gagagccgcg aggcgatccg cgccctgcac
    10801 gaccggggca tcgaggtcgc gatgctgacg ggcgacgcgc gcgccgtcgc ggatgccgtg
    10861 gcggaagatc tcgggatcga cacggtcttc gccgaggtgc tccccgagga caaggcgtcg
    10921 aagatccgcg agctgcagga gggggggcgt cgggtcgcga tggtgggcga cggcgtgaac
    10981 gacgccccgg cgctcgccac cgccgacatc ggcatcgcca tcggcgcggg cacggacgtg
    11041 gccgtcgagg ccgggcacat cgtgctggtg aggtcggacc cgagggacgt cgcccggatc
    11101 gtcacgctgt ccagggccac gtaccggaag atgctccaga acctgtggtg ggcggcgggc
    11161 tacaacatcg tggcgatccc cctcgccgcg ggcgccctgg ccgcctgggg catactgctc
    11221 accccggccg tgggcgccgt gctgatgtcg gccagcacgg tcatcgtggc cgtcaacgcc
    11281 cagctcctca agcggacgcg cctgtgacgc ggccgggatc gccccacgcc ggaaacggcc
    11341 attcgccccg tggaattcgg gaggagcaat ctgcattctg tcgtatcccg acccatcagc
    11401 caccggtgcc gatccaatgg ctcgccgtcc ggttctcgcg ctgctgcttc tgctgcccgc
    11461 cgtcacggcg cacgcacagg tcgacgatca cttcccgctg gccgtcggca atgcctggac
    11521 gtatttcacc ccgctggatt ccgtcccgcc ggataccgtg tgggaccata cgtcgggcgt
    11581 ggccgcggcc gtttcggtga atgacaccct gtatttcgtc gtcgattatc cgttcgcgcc
    11641 cgccgacacg ctccgcgcag acggagatgg aaaggtcttc gcgcgtctcg gcgggcgtga
    11701 cttgctcctc ttcgatttca ccgtggcggg gggtgagacc tacccgttcg cgagcccgag
    11761 tgccccggaa gtcaatttca ccgtgacggt cgatcgcctg gacatgtgcg aggcggcggc
    11821 cggccggttc ccggactgcg tccgcctggc cttcgacgat ccggaatggt cggacgagga
    11881 attcaccttc gtcttcgccc agggggtggg aatagtctgg gcgtacggtg acggcggatg
    11941 gtacgaggag cttttcgccg cgcgggtggc cggccgcatc atcacgtcca gggagtgggc
    12001 cgcgtcgacc gccgcgcagg ccttcgcgta tccgaatccc ttcggggact cggcgaccat
    12061 cgtggtgccc gcgacgggtg cggcgacgat gcgcgtgcgg gtctacgacg ttctcggtcg
    12121 gctcgctgcg acgccgggcg aggggatgtg cgacggcctc acgtgccgat tccgactcga
    12181 cggagccctt ttgccggccg gaccgtactt cgttcgggcg gagcagggtg gacacgtacg
    12241 tacgatgcgc ctgatcttgc gtcgatgacg cgacccgatc gacgcgccgc acctggatcc
    12301 atccgcgagt ccggctccac ggtccgcctg gcgcgggtcg tcgggatccc cggcgccgtc
    12361 ctgatgggcc tcggctccat cctgggcacc ggcatcttcg tgagcatcgg cattgcggcc
    12421 ggcgtggcgg gtccgtccgt ggtcctcgcg gtcgccctgg cggcggtcgt cgcggcgttc
    12481 aacggcctct cgagcgcgca gctcgcggcc ggccacccgg tcagcggcgg aacctacgag
    12541 tacggatatc ggtggctgac gccgacgctc ggctttacgg cgggatggat gttcctcgcg
    12601 gcgaagtcgg catcggcggc cacggcggcg ctcggactct ccggctacgt gctgacggcg
    12661 ctgggtctcc ccggcgccgg gccccgaatc gccctggcca tcggctcggt cgccgtcctc
    12721 acgatgatcg tcgcgtccgg aatgagccgg tcgaaccggg caaacgcggt catcgtctcc
    12781 gtgacgctcg cggccctcgc cgccttcgtc gtcgccggcc tcccgtcggc cgcggacggc
    12841 gccgctctga acgtgtccgg gctcttcgat gccggcggcc tgcagcgcct tctgcacgcc
    12901 acggccctca tgttcgtcgc gtacacgggc tacggtcgca tcgccaccct gggcgaggag
    12961 atccgcgatc cggcgcgcag cattccccgg gccatcatcg tcacgttgct ggtcacgatg
    13021 ggactctacg tctcggtgac cctcgtcgcg gtggctgccg tcggcgctcc cgccatggcc
    13081 gaggccacgg gggtcgccgc ggccccgctc gaggtcatcg cccgcggctt cggcgtgccg
    13141 ggcgtgggct ggatcgtcgc ggtgggtgcc gtgacggcca tgctcggagt cctgctgaac
    13201 ctgctcctcg gcctgtccag ggtgctgctc gccatggcgc gccggggcga catgccgcgg
    13261 ggtctcgccc gcgtcgaggg cattcgcgcg tcgccgcgcc gggcggtgtt cgccgtgggt
    13321 gccgtgatcg cgctcctcgc actgacgggc agcgtgagga cgacgtggtc cttcagcgca
    13381 ttcaccgtgc tcgtttacta cgcgatcacg aacctggcgg cgctgcggct gccggcggac
    13441 ctccggctct atccacggtg ggtcgccgcc gcgggcgcag cgtcgtgcct gggcctcgct
    13501 ttctgggtcg agccgcggat ctggctggcg ggcctggggc tgattgccgt cgggctggtg
    13561 tggcgcggtg tggcccggag gccttgaagg gccctaccgc cccgcctcca ggtaccgccg
    13621 gatcccggcg aacgtgtctc tccagcccgt gtcgcaggcg gcgtagaagt cgtccgccac
    13681 ggcggcgtcc gggaagccgt cctgggtgac gcgcagaatg gagccgtcgg gatggggctc
    13741 cacctcgaag gtcgtcacga agctcgcctc gaagggaagc gggccggagc gggcgaaata
    13801 ccgcgaatcg ccgaacgcga gccgccgcgg tggatcgaac tcgacgaggc gggccgccga
    13861 gacgtagtcg gggtcgtcgt cggactcgcc ccacgaggcc gtccagtatc ccgcctccac
    13921 tgcgagcacg acggccgtgc gggcgcccca ccacgcgcag atggcgctcg gcgtgtgcag
    13981 cagccggaac accgcatcgg gcggcgcggg gatgacctct tcgtgcacgt ggcgtcgcat
    14041 gcggtcggaa cgcgcgcgtc cgcccgtggt tcagccaccg cacccgagcg gcccaccccg
    14101 tgctcaccaa gcgcatcatt ccctgcctcg acatcaagga cggccgcacc gtcaaggggg
    14161 tcaacttcgt ggggctccgc gacgcgggcg acccggtgga gcttggcgcg cggtacgccc
    14221 gcgagggtgc ggacgagctg gtcttcctcg acatcacggc gacgctcgag gatcgtgaca
    14281 cgttcgtggc gctggtgcgg gacatcgcga gccacctgga catcccgttc accgtgggag
    14341 gcggcatctc gcaggtgtcc cacgtcgcgg cccttctcga ggccggggcc gacaaggtct
    14401 cgatcaactc ggcgcccgtc cggcgccccg cgctcgtctc ggagctcgcc gccgagttcg
    14461 gcagccagtg cgtggtgctc gcggtcgaca cgcgtcggct tggcggccgc gacctcgtcc
    14521 acacgcatgg cggcacccgt ccgacggaaa tcgaaacggg ggtatggatc cgcgaggcgg
    14581 tggatcgtgg agcgggcgag atcctgctca cctccatgga ccacgacggc acgaaggagg
    14641 gcttcgccgt cgacatcacc cgcgccgtat cgcaggacgt tcccgtcccg gtcatcgctt
    14701 cgggcggcgc gggagcgcgc gagcatttcc tcgccgtctt cagggacggc ctcgccgacg
    14761 ccgcgctcgc ggcatcggtc ttccattttg gcgagattcc ggtgccggag ctcaaggcat
    14821 accttcacga aaacggcgtt cccgtccgca tctgaaccat ggacatcgac ttcgagaagg
    14881 gtggcggcct ggtacccgcc atcgtccagg atgcccgcac gggcacggtg ctgatgctcg
    14941 gttacatgag ccgggaggcg ctggaccgca ccgtccagac ggggctcgtg accttcttca
    15001 gccgcgagcg gggcgtgctc tggaccaagg gcgagacgtc gggcaacacg ctcgccttcg
    15061 agtccatggc cgtcgactgt gacggcgaca cgctcctggt gcgcgcccgc ccgtccggac
    15121 cggtctgcca cacgggtacc gacacgtgct ggggcgagtc gaacgagccg gacccgatgg
    15181 ccttcctctc ggagctcgac gccgtggtcg ccgagcggtc gggtgcgccg ccggaagagt
    15241 cgtacacccg tcgccttctg gacggcggcg cggcggcggc cgggcagaag gtgggcgagg
    15301 aggccgtcga gaccgtggtg gccgccctcg cgcaggacga cgggcgcgtg gtggacgagg
    15361 cggccgacct cgtctaccac ctggtggtgc tcctgcgagc gcgcggcctc tcgctgggcg
    15421 acgtcgcgct gcgcctcgag gagaggcacc ggccaggccg gtagaccgtt ggacgggcgg
    15481 cacggccggt acggaccccg tgacggacga ttcgctccgg gggggttccc ctaatgctcc
    15541 ccgtcggcta gtttcgacgg catcacgtcg aggacggtgc cgatgcctca ttcctgccgc
    15601 ctggccctgg tgctcgccgc actcctgctc ccgggtccgg cgctcgcaca gaccggcacc
    15661 gttcgcgggt tcgtgacgga ggctcggagc gagggcccgc tccagggcgc caacgtgtcg
    15721 ctgctcgttc cgggcggcga gacgcgaggg acggtgacgg atggtgacgg ctacttccgc
    15781 atacccgccg tgccggccgg cgagcagcgg atcgtggtca cgtacatcgg gttccggccc
    15841 tacgacgaga cggtccgggt gccggacggg gggacgatca cgctgcgcat cgccctcgag
    15901 gagtccgcgg aggtcatgga ggaggtcgtg atcgaggcgg agggcggatc gggcgtggcc
    15961 gccgtgacgg ccggcctgca ggcgatccgg ccgcgggacg tcgcgcgggt gcccattcac
    16021 ggagcgtcgg gggacctggc ggcctatgtc cagtccgtgc ccggcgtggt cctgccgggc
    16081 gatcgcggag gccggttcca catccgcggc ggctccgacg accagaacct ggtactggtc
    16141 gagggcatcc cggtctacgg cgccttccac atcctgagcc tgtattcggc ctttccggac
    16201 gaggtgatcg accaggccga cttctacacg gccggcttcg gcgccaggta cggggagcgt
    16261 ctgtcctccg tgctggacgt gcggacccgg aacggcaaca agcaggcgtt cggaggctcg
    16321 gcgtcgctct cgccgttcct gagcggtgtc cgcctggagg ggccgatcat cccgggccgg
    16381 gcgtccgtgc tcctgtccgg tcgccggtcc ctcgtcggag acctcatgcc gtcggtcctc
    16441 gggcagaaca tgccgtaccg cttcggcgac ggtttcggaa agctccatgc gttcctgggg
    16501 tcggggcact ccctgtcggt cttcggcctg ctgtccgacg acgaggggca gatcgccggc
    16561 acccagtaca cgttcgacgg ggagctcgtg cagacgcccg ccaccgattc gctggacctc
    16621 cggtgggaca accgcgtggt cggcggcacc taccggctgg agccggggtg ggggcagccc
    16681 gtgagggcgg agttcaccgt cggtcactcg cgctggcgct cgtacttcgg gccgacgggc
    16741 gaggaggcga ggcagacggg gctccagagc ctggacatcc ggctggacct gggcttcgtg
    16801 gtgagcggcg tggatgtgcg gctcggcacg ctgcggcggt ccaccgacgt cacgtaccac
    16861 ctggacgatc ctttccgcga ggcgcccgaa gcgtatgacc gcaaccgggt ggagcggtcg
    16921 gactacgcgc agctggacat cccgctgcgt gccctgggac tctggatcca gccaggggtc
    16981 cgggcctacg gggtcggcga ggaacccgtc ctgtgggagc cgcgcgtccg gtccacgctc
    17041 gagcttccgg gcttcgacgg cacgcaccgg ctgacggcgg cctgggggcg gatgcatcag
    17101 tcgctcgtcg ggctcgtcga cgagcgggat gtgggcaatc cctttgccgt ctggacggtg
    17161 agcgaggcgg atcgcccggt gcccctggcc agccacgccg tcgtgggatg gaacgcggcc
    17221 ccgctccggg acatccaggt cgcggtcgag gtcttcgaga agcggttcga acacctccag
    17281 gtgcccgtct tcagcgcctt cccgacgttc acgaccgctc tgcagacggc cgacggcagg
    17341 gctcggggag tggacgtccg ggtgtcgttc gagaatgtcc ccgggccgta cgagtcgcga
    17401 gtcggcggat gggtgagcta tgccctcgcc gaggtcgagt acgagaccgc gaacggcacg
    17461 gagtatgcgc ccgcccacga ccgcaggcac agcgtgcagg ccgtggtcga cgcgacgaag
    17521 gacgacatgg cgctgctggc ccaggtccag gtcgggtcgg ggcttccata cactccgtcc
    17581 tccggtttcg acacctggta cctgatgacg ccgggcgtcg acgtcgcgtc cgatcccggc
    17641 cgtcagcggg tcctctacgc gcggcagaac agcgaacgcc tcccgctgta cgcgcgcctg
    17701 gacctgtgga tcgagcggcg catcgaccgc ggccgctcgg tgatcacgct ccgcgccggc
    17761 ggcaccaacg tcttcaaccg tgccaacctg ttctatttcg acctgttcac gtaccggcgc
    17821 gtggaccagc ttcccttcat gccctccgtg ggcgtcagga tcgagttccg atgattcgac
    17881 gtctcctctt cctggtcgcc gggctggcgg cgctcgccgc agggtgcgac gatcccttca
    17941 tcgacccgtt cgccaacgag gaccgctggt tcaccgtctg gggatggatc gatcccgacg
    18001 cggcgcgcca ggaagtgcgg gtcatccccg tcaccaggcg tccgtcgacc attgcgagcc
    18061 cggacgacat gaacgcggcg gtcaacgccc gggtcttctc gatcgacctg ggctcgggca
    18121 ccgagaccga gtgggcgtac cgcctggcgc agctggacga cggcacgtat ggccacgtgt
    18181 tctccgcgtc cctgttcgtg cggcccgccg acagcctgcg gctcgaggtc cggcgttcgg
    18241 acggccgcgt cacgtgggcc gagacggtgg ttccgacgcc cacgccgatc tcgctcggcg
    18301 acatcggagt cgtctacgag gcggagaacg gccacctggc gcaggacatc gtcgtacgcg
    18361 gggtcgagcg gccctggagc ctgcgcgtca cgtaccgcgt ggaggggagc ctcatcgaca
    18421 agtggttcaa gacggtggac tacggtcgca gcggccggtc ggacggccag gggaactgga
    18481 tcttcacggt cgacctgacc gccgaccagg aggcgatccg ggaggacatc cgggccgccg
    18541 tcgaagcggg cgccgtgccg cgcccggagt tcggggacat tcccgacgcg gggctcaacc
    18601 gcatgaacgt cggggtgctc gagctcgacg ggacgtggga cctgccgttc ggggagtacg
    18661 acgacgaggc gtacgccttt ccggacgcgc tcacgaacgt ggtgaacggg tacgggtact
    18721 ggggctcggg gtcgcgggtg tcccagcagt gggaggtcga ttcgatcctg tcgcggcgcc
    18781 tggggtggcc gttctgaggc gcggaatcac tccgggaaca gggacgggtc cagccccggg
    18841 atgatgcgga gcgcgttccg gtagtagatg tcgcgcagca cgtcgtccgg gaggtccagg
    18901 ccgtacaggc gccagaaggc gtggtacttc cggtagtagg ggaagtactc gtcggcggtc
    18961 tcgaacgtgc ggaagtacgt gtggtactcg aacggccggt aggtgtcctt gccgaagagg
    19021 acccggtcgt ggtaccggac caggaactcc cgggcgcggc gcggctgtcg gcccagctcc
    19081 tgcacgacgg cgcccacctc ggtgaccacg ttgggcaggg cgtcgagcat cgcgcccagc
    19141 cggtccaggt cgctgccgaa ccagccgaga tgggcgttga tgaaggtcgt tccgggatgc
//
LOCUS       DBNHQZ010000385         4913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1740304, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000385 DBNHQZ010000000
VERSION     DBNHQZ010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1740304"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2194
                     /locus_tag="AC16YU_10470"
     CDS             <1..2194
                     /locus_tag="AC16YU_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6065694.1"
                     /translation="MSGIVNVVTKEGSGQRFGGAVKVFAGSYATGGEGGADMLRGQQV
                     DEYTEAGIQYRDVDPYSYLSFEPTHFKNGQVSLEGPIFGDRLTFFALGRFFSNDGWLY
                     GARLYKPDGTAGDSSLVPMNNFERYSWQANVRWRLNRKMIVNLITLGQSSESRGFSHY
                     RRWAPDGRSFSFNEGLDAKIKFTHLLSATTFYTLNAAVFRSHNQSYRYEDPFDARYTD
                     FDLVPPDSVEVRPGEYESVLTGFGRFGRGGTDLGRFERETISYLVKGDVSSQLGGNHL
                     VKAGFQVKVDDMGLTGYSLQPAVDPATGQTIEPFRTAIPDSISSRFTWFQDFAPISVA
                     AYIQDKIEFENFIVNAGLRFDWFDSRAQIPADPADPNIFNPFRPTNRFNDLDGDGVIS
                     AEEETPGNRKTVAEREAYWWTDASPKAQISPRLGVAYPITEEGVIHFSYGIFFQIPTV
                     DQMSAGFGYKIANQSGSYGPFGNPDLDAQKTTMYEIGFKQGLGDFVVDVTGYYRDVRA
                     WVSTSVPILTYLPGINYVVFTNRDYANTRGVTLSLNRKFRDHFGFDLSYTYQVVDGSN
                     SNPSDEFFSLLGNAQPTLALLPLDWDQRHKFAGALYTGGDAWGGSLRFRLEDGFPYTP
                     SFTQAALVGNDVQPEFPRNSRRIPASFEVDVSVHYEFALRSLRPRVFVEVYNLLDSRS
                     VVGVFSDTGEPDVTLDQLRTGQFDAGYFVQPTNYSEPRRIQLGIDFRF"
     gene            2261..>4913
                     /locus_tag="AC16YU_10475"
     CDS             2261..>4913
                     /locus_tag="AC16YU_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065695.1"
                     /translation="MHRKAIGAALMALFGLQSGSALAQGVIDRNHVPSLERVDQFERR
                     RDNIDGNNVRASITNYAQTAQTGDPGDFWYEWPKNTGRRYISLTQLFVGARVTGINND
                     TLWVVDVANFRSNPVDENISWTFEPIKGYVNPADASFGIAQSDEPESWPPFWPDKQED
                     VADPGWIGSWNGFFGKDAFNADQEFFYKIGDDQYNRYPTYFPDSTDLTRKGLGTVAEV
                     RTLAWSQILIDDVVFHIHGIKNDGTEPLTQVGFSMWLADLVGGDASDDIPFFDLLEDV
                     AFMTDADGIGDEFFGSDPVGQAVFAFLETPGNAVDRIDNDADGSTAAECLPSEGECSS
                     PVVPIEWLVGENPTNGRDDNGNGLIDENETHAPFDGEQGASPGVGYADYLDNDLDGEQ
                     GGPVVTAAMVAAATPDAFLRWPPNPGGDAVSTKMNGNPIVHLLGVGEEDLGVPFRDNI
                     DNDDSCVEPTPAYPYLSEPGSPVVTQAIVDAAAADPYRRYRVPGTDIVLYDVGTEDLG
                     ACYADGVDNDLDGAVDEGIDEGFDEMIDESRSDGIDNDGDWNALQNDTGLDGVAFNGD
                     PGDSDGLPTTGAGTDFPGERNIDVTDISESDQIGITNVQIIPAFFLNLNGQTDRFLFN
                     SFMIPGDFDADLPSPGENDLVVSSGLFPLEPGQTERISLSIGLGLTRAEVLQSRGYAL
                     QAYLEDYQFAQAPITPELSAVPGDGRVTLYWDSDAEESYDQFLAGLGRNPNDFEGYRV
                     YRSTDPAFLDALTITDGFGNATLRKPIAQFDRIDAFEGFHPTDINGLKFYLGNNRRDP
                     GEDAAGLAHTYVDTDVTNGVRYFYAVTAYDFGSAVDNIPPTETPIRIQRLADGTIRTG
                     PNVVAITPTAAAAGMTDAIIESIDRTKG"
BASE COUNT          864 a         1688 c         1594 g          767 t
ORIGIN      
        1 gatgtcgggc atcgtgaacg tcgtgacgaa ggaggggtcg gggcagcggt tcggcggagc
       61 ggtgaaggtg ttcgccggct cgtacgcgac ggggggcgag ggtggcgcgg acatgctccg
      121 cggccagcag gtggacgaat acacggaggc gggcatccag taccgagacg tggatcccta
      181 ctcctacctg tcgttcgagc cgacgcattt caagaacggc caggtctcgc tcgaggggcc
      241 gatcttcggt gaccgcctga ccttcttcgc gctgggccgc ttcttctcga acgacggctg
      301 gctctacggc gccaggctct acaaacccga cggcacggcc ggtgactcct cgctcgtgcc
      361 catgaacaac ttcgagcggt actcctggca ggcaaacgtc cgctggcggc tgaaccggaa
      421 gatgatcgtg aacctgatca cgctcgggca gtcgtcagag agccggggat tcagccacta
      481 ccgccggtgg gcgcccgacg gccggtcgtt ctcgttcaac gagggcctgg acgccaagat
      541 caagttcacg cacctcctga gcgcgaccac gttctacacc ctgaacgcgg ccgtcttccg
      601 ctcgcacaac cagtcgtacc gctacgagga cccgttcgac gcgcgctaca cggacttcga
      661 cctggtcccg cctgactctg tggaggtccg cccgggcgag tacgagagcg tgctcacggg
      721 cttcggccgg ttcgggcgcg gcgggaccga cctggggagg ttcgagcgcg agacgatctc
      781 gtacctggtc aagggcgacg tgtccagcca gctgggcggc aaccacctgg tcaaggcggg
      841 cttccaggtg aaggtcgacg acatggggct cacgggctac tcgctgcagc cggccgtgga
      901 cccggcgacg ggccagacga tcgaaccctt ccggacggcg atcccggatt ccatctcgtc
      961 ccgcttcacg tggttccagg atttcgcgcc gatctcggtc gcggcctaca tccaggacaa
     1021 gatcgagttc gagaatttca tcgtgaacgc gggcctgcgc ttcgactggt tcgactctcg
     1081 ggcgcagatc ccggccgacc cggccgaccc gaacatcttc aacccgttcc gcccgaccaa
     1141 ccggttcaac gacctggacg gcgacggcgt gatctcggcc gaagaggaga cgcccgggaa
     1201 ccgcaagacc gtggccgagc gcgaggccta ctggtggacc gacgcgtcgc ccaaggcgca
     1261 gatctccccg cgcctgggcg tggcctaccc gatcaccgag gagggcgtga tccacttctc
     1321 gtacggcatc ttcttccaga tcccgacggt ggaccagatg tcggccgggt tcggctacaa
     1381 gatcgcgaac cagagcggca gctacgggcc cttcggcaat ccggacctgg acgcacagaa
     1441 gaccaccatg tacgagatcg gcttcaagca ggggctgggc gacttcgtgg tggacgtgac
     1501 cggctactac cgcgacgtgc gcgcctgggt gtcgacgtcc gtccccatcc tcacgtacct
     1561 gcccggcatc aactacgtgg tcttcacgaa ccgtgactat gcgaacacgc gcggagtgac
     1621 cctgtcgctc aaccggaaat tccgggacca cttcgggttc gacctgagct acacctacca
     1681 ggtggtcgac gggtcgaatt cgaacccttc ggacgagttc ttctcgctcc tgggcaacgc
     1741 gcagcccacg ctcgcgctgc tgcccctcga ctgggaccag cgccacaagt tcgcgggcgc
     1801 gctctacacg ggcggggacg cctggggcgg ctcgctgcgc ttccgcctcg aggacggatt
     1861 cccgtacacg ccgtcgttca cgcaggctgc gctcgtcggc aacgacgtcc agccggaatt
     1921 cccgcgcaac tcgcggcgga ttccggcctc gttcgaggtg gatgtgtcgg tgcactacga
     1981 attcgccctt cgatcgctgc ggccccgcgt gttcgtcgag gtctacaacc tgctcgactc
     2041 gcgcagcgtc gtgggcgtgt tctcggacac gggtgagccc gacgtcacgc tcgaccagct
     2101 gcgcacggga cagttcgacg ccggttactt cgtgcagccc acgaactaca gtgagccccg
     2161 ccgcatccag ctgggcatcg acttccgctt ctgatcccga gactccgatc accccggggc
     2221 cagcccggga tcgccacctt tcggaatgac ccgatccaac atgcaccgca aggcaatcgg
     2281 cgccgcgctc atggcgctgt tcggactcca gtccggctcc gccctcgcgc agggcgtcat
     2341 cgaccggaac cacgtgccca gcctggagcg cgtggaccag ttcgagcgcc ggcgcgacaa
     2401 catcgacggc aacaacgtca gagcctcgat caccaactac gcccagaccg cgcagaccgg
     2461 ggatcccggc gacttctggt acgagtggcc caagaacacc ggccgccgct acatctcgct
     2521 gacgcagctc ttcgtgggcg cgcgcgtcac cggcatcaac aacgacacgc tctgggtcgt
     2581 agacgtggcc aatttccgct cgaacccggt cgacgagaac atctcgtgga ccttcgagcc
     2641 gatcaagggc tacgtgaacc cggccgatgc gtcgttcggc atcgcccagt cggacgagcc
     2701 ggaatcctgg ccgcccttct ggcccgacaa acaggaggac gtcgccgacc cgggatggat
     2761 cggctcgtgg aacggtttct tcgggaagga cgccttcaac gccgaccagg agttcttcta
     2821 caagatcgga gacgaccagt acaaccggta tccgacgtac ttcccggact cgacggacct
     2881 cacacgcaag ggtctgggca cggtggccga ggtcaggact ctggcctgga gccagatcct
     2941 gatcgacgac gtcgtcttcc acatccacgg gatcaagaac gacgggacgg agcctctcac
     3001 gcaggtgggc ttctccatgt ggctggccga cctcgtgggc ggtgatgcgt ccgacgacat
     3061 cccgttcttc gacctgctcg aggatgtcgc gttcatgacg gacgccgacg gcatcggcga
     3121 cgagttcttc ggatcggacc cggtcggaca ggccgtgttc gcattcctgg aaacgccggg
     3181 caatgccgtc gaccggatcg acaacgacgc ggacggctcc acggcggcgg aatgcttgcc
     3241 gtccgagggc gagtgctcga gcccggtggt gccgatcgag tggctggtgg gcgagaatcc
     3301 caccaacggc cgggacgaca acgggaacgg gctcatcgac gagaacgaga cgcacgcccc
     3361 gttcgacggc gagcagggcg cgagcccggg tgtcggctat gccgactacc tggacaacga
     3421 cctggacggc gagcagggcg gtcccgtcgt gacggctgcg atggtggcgg ccgccacgcc
     3481 cgacgcgttc ctccggtggc ctccgaatcc cggtggcgac gccgtgtcga cgaagatgaa
     3541 cggcaacccg atcgtccacc tgctcggcgt cggcgaggag gacctgggcg ttccgttccg
     3601 ggacaacatc gacaacgacg actcgtgcgt ggagccgacc ccggcctacc cgtacctgtc
     3661 cgagcccgga tcgcccgtcg tgacgcaggc catcgtcgac gccgcggccg ccgacccgta
     3721 ccgccggtac cgcgtgccgg gcacggacat cgtcctgtac gacgtcggca ccgaggacct
     3781 gggcgcgtgc tacgcggacg gggtggacaa cgacctcgac ggcgccgtgg acgaggggat
     3841 cgacgaggga ttcgacgaga tgatcgacga gtcgcgctcc gacggcatcg acaacgacgg
     3901 cgactggaac gcgctgcaga acgacacggg actcgacggc gtggccttca acggagatcc
     3961 gggcgactcg gacggcctgc cgacgacggg cgcgggcacg gacttcccgg gcgagcgcaa
     4021 catcgacgtc acggacatct ccgagtcgga ccagatcggc atcaccaacg tgcagatcat
     4081 tccggccttc ttcctgaacc tgaacggcca gacggaccgg ttcctgttca actcgttcat
     4141 gatccccggc gatttcgacg ccgacctgcc gtcgcctggc gagaacgacc tcgtggtctc
     4201 gagcgggctc tttccgctcg agccgggcca gaccgagcgc atctcgctct cgatcgggct
     4261 cggcctcacg cgcgccgagg tgctgcagtc gcgcggctac gcgctccagg cgtacctgga
     4321 ggactaccag ttcgcgcagg ccccgatcac gcccgagctc tcggcggtgc cgggcgacgg
     4381 ccgcgtgacc ctgtactggg attcggacgc cgaggagtcg tacgaccagt tcctggccgg
     4441 cctgggccgc aatcccaacg acttcgaagg ctaccgggtg taccggtcca ccgatccggc
     4501 cttcctggac gcgctcacga tcacggacgg gttcggcaac gcgacgctcc gcaagcccat
     4561 cgcccagttc gaccggatcg acgcgttcga aggcttccac ccgacggaca tcaacgggct
     4621 caagttctac ctgggcaaca accggcgcga tccgggggag gacgccgcgg gcctcgcgca
     4681 tacgtacgtg gacacggacg tgacgaacgg cgtccggtac ttctacgcgg tcacggccta
     4741 cgacttcggc tccgccgtcg acaacatccc gccgaccgag acgccgatcc gcatccagcg
     4801 cctggccgac gggacgatcc ggaccggccc gaacgtggtc gcgatcacgc cgacggccgc
     4861 cgcggccgga atgacggacg ctatcatcga gtcgatcgac cggacgaagg gca
//
LOCUS       DBNHQZ010000386         2428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2417826, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000386 DBNHQZ010000000
VERSION     DBNHQZ010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2417826"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..>2428)
                     /locus_tag="AC16YU_10480"
     CDS             complement(362..>2428)
                     /locus_tag="AC16YU_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR6065696.1"
                     /translation="NAVVIWDPMLNPDGRDRFVDGVNRFQTRVPTSDGEDMEHREPWP
                     GGRTNHYWFDLNRDWLPAEHPESRGRLELYHAWRPQLLTDHHEMGSGSTFFFQPGIPS
                     RRNPKTPEQNVRLTEELATYHAAALDRIGSQYYSEESFDDFYYGKGSTYPDINGAIGI
                     LFEQAGSRGLLRETSDGELSYAFTVRNQFTAAVSTLTGAVAMRTRLLAHQRDFYAGVP
                     AWARSNGVKGWVVSTERAPTRARMLLELVGRHGIEARRLARPIDVDGRRFSPESAYVL
                     PVAQTQGRLLDAMFDPVTTFLDSIFYDVSAWSLPLAYDVDHAAVRSMPSDLIGEAFGP
                     TDIPGGTVIGGAGRSPAAWLMSWDRYFAPRAMYRLQSEGVAPRLATRPFTALVGGRAV
                     RFDRGTVVIPVLPREAAGRGSQGPDVDALVRELAGSEGVTFHAADTGLTPEGPDLGGG
                     STEVLTMPRIALLAGDGTSAYDAGPTWLYLGERLRLPISLLEPDRIARADLSRYNVIV
                     LAGGSYDGLDAAALREWTRKGGTVIGMGSAVNWLARNEFVSVERRESTPDSVLAGLPY
                     AELSTARGAREVGGAILSAALDTTHPLAYGLPAALPVFRQGDSYYDADTPPGEVFGRY
                     SDSPLLAGYLPRSGSDRAAGAVAALARREGDGRVIAFSDVLTFRGYWHGTERLLANAI
                     FLREAF"
BASE COUNT          385 a          842 c          865 g          336 t
ORIGIN      
        1 tctgtgaaac aggtttgcgt agaggccgcg cagcggccga ccatggagcg ggcgaagccc
       61 gcggaataat cccgtcgggc ccacatgctg cgaccccgtc gtccgaagga tggcggggtc
      121 gcagcatagt tagcggtggg gtgagcgcag agcacgcgga gcgtgcaaga atgaaggccg
      181 gaatgacctg cgaaacaggt ttgcgttgag gccgcgccag cggccggcca tggagcgacc
      241 gacaggcgcg gagtcatccc gagggccccg tagagcccgt cccgggtgtc actgggcagg
      301 cgcgaaacgc gccggcactt ccagtccact accacctgca atccgggagc gactcgctcc
      361 ctcagaacgc ctccctgagg aagatcgcgt tggccaggag ccgctcggtg ccgtgccagt
      421 acccccggaa ggtgaggacg tcggagaacg cgatgacgcg cccgtcgccc tcccttcggg
      481 cgagcgccgc caccgcgccg gcggcgcgat cggaccctga ccggggcagg tagccggcga
      541 gcaggggcga gtccgagtac cgtccgaaca cctctcccgg gggggtgtcg gcgtcgtagt
      601 acgagtcccc ctgccggaag accggcagcg cggccgggag gccgtatgcc agcggatgcg
      661 tcgtgtcgag tgcggccgac aggatggcgc ccccgacctc ccgggctccg cgcgcggtcg
      721 agagctcggc atacggaaga cccgcgagga ccgagtccgg cgtcgactcc cggcgctcga
      781 cggagacgaa ttcgttgcgc gccagccagt tcacggcgga ccccatcccg atgactgttc
      841 cccctttccg ggtccactcc cggagggcgg ccgcgtcgag cccgtcgtag gacccgccgg
      901 ccaggacgat gacgttgtac cgggacaggt ccgcccgcgc gatccggtcc ggttccaaca
      961 gggaaatcgg gagacgcagg cgctcgccca ggtacagcca cgtcggcccc gcgtcatagg
     1021 cgctggtgcc gtcgccggcc aggagcgcga tgcggggcat cgtgagcacc tccgtgctgc
     1081 cgccacccag gtcgggccct tccggggtga gccccgtgtc ggccgcgtgg aaggtcacgc
     1141 cctcggaccc ggccagctcc cgcaccaggg cgtcgacgtc cgggccctgg gacccccgtc
     1201 cggccgcctc gcgcggtagg accgggatca ctacggttcc gcgatcgaac cgcacggcgc
     1261 gcccgccgac cagcgcggtg aagggccgtg tcgcgaggcg cggcgcgacg ccctcggact
     1321 gcaggcggta catcgcacgg ggcgcgaagt agcgatccca tgacatgagc caggccgcgg
     1381 gagaacggcc cgccccgccg atgaccgtgc cacccgggat gtcggtcggc ccgaacgcct
     1441 ctccgatgag atcggagggc atcgaacgga cggccgcgtg gtcgacatcg tacgccagcg
     1501 gcagcgacca cgccgagacg tcgtagaaga tcgaatccag gaatgtcgtc accgggtcga
     1561 acatggcatc cagaagacgg ccctgcgtct gcgcaacggg gagcacgtaa gccgattccg
     1621 gcgagaagcg tcggccgtcg acgtcgatcg gtcgggcgag acgccgcgcc tcgattccgt
     1681 gccgtccgac gagctcgagg agcatccggg cgcgggtcgg cgcccgctcg gtggagacga
     1741 cccacccctt gacgccgttc gagcgcgccc aggcgggcac gccggcgtag aaatcgcgct
     1801 ggtgcgcgag gaggcgggtc cgcatcgcga cggccccggt cagggtcgag acggccgccg
     1861 tgaactggtt ccgcaccgtg aacgcgtagc tcagctcgcc gtcggacgtc tcccgcagca
     1921 ggccgcgaga tccggcctgc tcgaagagga tgccgatcgc gccgttgatg tccgggtagg
     1981 tcgatccctt gccgtaatag aaatcgtcga acgattcctc ggagtagtac tgcgagccga
     2041 tccggtcgag cgccgccgcg tggtaggtgg ccagctcctc cgtcaggcgg acgttctgct
     2101 ccggcgtctt cggattgcgc cggctcggga tgcccggctg gaagaagaag gtgctgccgg
     2161 agcccatctc atggtggtcc gtcagcagct gcggtcgcca cgcgtggtag agctcgaggc
     2221 gccccctcga ttccgggtgc tccgccggta gccagtcacg gttcaggtcg aaccagtagt
     2281 ggttcgttcg accccccggc cacggctccc ggtgctccat atcctctccg tcgctcgtgg
     2341 gcacccgggt ctggaagcgg ttcacgccgt ccacgaaccg gtcgcgcccg tcgggattca
     2401 gcatcgggtc ccagatgacg accgcgtt
//
LOCUS       DBNHQZ010000387         3690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_792120, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000387 DBNHQZ010000000
VERSION     DBNHQZ010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_792120"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..213
                     /locus_tag="AC16YU_10485"
     tRNA            136..213
                     /locus_tag="AC16YU_10485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Pro,seq:tgg)
     gene            231..304
                     /locus_tag="AC16YU_10490"
     tRNA            231..304
                     /locus_tag="AC16YU_10490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:265..267,aa:Arg,seq:tct)
     gene            330..400
                     /locus_tag="AC16YU_10495"
     tRNA            330..400
                     /locus_tag="AC16YU_10495"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:362..364,aa:Cys,seq:gca)
     gene            519..2528
                     /locus_tag="AC16YU_10500"
     CDS             519..2528
                     /locus_tag="AC16YU_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HIR6065697.1"
                     /translation="MRLRLALPVTAMILAALGSVPGARAQRDPGFGRAPLASVPYATL
                     PLGAIRPEGWLREQLRRMADGMTGHLDEWYPTVGSSNGWVGGDGDVWERGPYWLDGLV
                     PLAFLLEDEALVAKARPYVEWTLASQDQSGFFGPRPEAEGTENRPQEQRLNAADWWPR
                     MVMLKVLQQWHEATGDPRIIEFMTRYFRYQLDRLDAEPLDHWTGWGAARGGENQASVH
                     WLYNRTGESFLLDLSRKLFEQTVDWTGNFEDRASAEDYWRTHVVNVAMGIRQPAQEYV
                     RTGSERYLRAVEQGMAALMEKHGQAVGMFSGDEMLHGTDPVHGIELCAVVEFMLSLET
                     LMTVTGDVAFVDRLERVAYNALPTQVTGDQAGRQYFQQVNQVRLSSGDHGIFFEGYED
                     ATCFGLLTGYPCCTTNLHQGWPKLTQHAWLRSADGGLAALVYAPTSVRARVGGATVAI
                     REVTSWPMEDTVRFQVTEADAVRFPLHLRIPAWAVGARLTVNGEPGGRFEPGTIAVVD
                     RTWSAGDEVVLALPADIEVSRWHENGIAVHRGPLQFALPIDAVMRPALAKNPGTNSLE
                     ILEAHPTGEWNYGLTVDAGHPLQGFEIVRDPRPAAWPWTEAGAPIRLRATGYPIPSWR
                     EYNSAAGPMPPSPVRARLGTPRTIELVPYGATTLRIAVFPEVRPR"
     gene            2534..3604
                     /locus_tag="AC16YU_10505"
     CDS             2534..3604
                     /locus_tag="AC16YU_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6065698.1"
                     /translation="MTDPAVAVQRKFLIAVVIVVTILFLWMVRSFLIAMFMAAIFSAM
                     ARPLYRRMTRWCRGRTSLAAGLTLVVLIVAVGLPMSGFVTLVVSQAIDITEAARPWVE
                     RQITAPGAWDRLVEQFPLLSRIVPPGTDVAAKLGEFATSVGEFLANSMVDVTRGTAAF
                     FLQLFVMLYAMYFFLVGGRVTLERIIGVIPLDQDTSRALVAQFVSVTRAVLKGSLIIG
                     VIQGVMAGGAFWIAGIQGWAFWTTIMIVMSVIPAIGSALVWIPAVIYLLLNGDVMAGV
                     LLAIWCAAVVGSVDNFLRPILIGRDTKMPDLLVLVGTLGGIFLFGALGFILGPIVAAL
                     FLAVWDLYGAEVREAPPEPQRP"
BASE COUNT          525 a         1266 c         1295 g          604 t
ORIGIN      
        1 tccgtttccg ctccaagatg gtccaccagg acgagctcca ggccgagatc aaggcggccc
       61 gacaggcctg atcgattgcg gccgtccgca tcgcgaccta tcgtggctcc ccggggtggc
      121 ccaggccgtc cctctcgggg tgtagcgcag cccggttagc gcgcctgctt tgggagcagg
      181 aggtcgcgag ttcgaatctc gccaccccga ccagcgtacc ttgccaacct gcgcccgtag
      241 ctcaacggga tagagcagcg gtcttctaaa ccgcaggttg caggttcgag tcctgccggg
      301 cgcgcgattt tcgtaccttg tgataccagg gcgacgtggc cgagtggcta ggcaagggtc
      361 tgcaaaaccc tgtacggcgg ttcgaatccg cccgtcgcct cacacgaagg accgcaggcc
      421 accgagtctg cggtccttcg tagcttacgg cgcggtttcg aagcgtcacg gttcgacagg
      481 aggccgcagc tccacccacg accgatcggc aagccgccat gagactccgc ctcgccctgc
      541 cggtgaccgc catgattctc gccgccctcg gatccgtgcc cggggcgcgg gcccagaggg
      601 atcccgggtt cggccgggcc cccctcgcgt ccgtgccgta tgcgaccctg cccctcggag
      661 cgatccgccc cgaaggatgg ctgcgcgagc agctgcggcg catggccgac ggcatgaccg
      721 ggcacctgga cgagtggtat ccgacggtgg gctcgtcgaa cggatgggtg ggaggcgacg
      781 gcgacgtgtg ggagcgcggg ccctactggc tggacgggct ggtgccgctc gctttcctcc
      841 tcgaggacga ggcgctggtc gcgaaggccc ggccctacgt cgagtggacg ctcgcgagcc
      901 aggaccaaag tgggttcttc gggccgcgcc cggaggcgga gggcacggaa aaccgtccgc
      961 aggaacagcg cctgaacgcc gcggactggt ggccgcggat ggtgatgctc aaggtgctgc
     1021 agcagtggca cgaggcgacg ggcgacccgc ggatcatcga gttcatgacc cgctacttcc
     1081 gctaccagct ggaccgcctg gacgcggagc cgctggacca ctggacgggc tggggagccg
     1141 cgcgcggcgg cgagaaccag gcgagcgtgc actggctgta caaccgcacg ggcgagagct
     1201 tcctgctgga cctgtcgcgg aagctcttcg agcagaccgt cgactggacc ggcaatttcg
     1261 aggaccgcgc ctccgcggag gattactggc gcacgcacgt ggtgaacgtc gcgatgggca
     1321 tcaggcagcc ggcccaggag tatgtccgga cgggctccga gcgttacctg cgcgccgtag
     1381 agcagggcat ggccgcgctc atggagaagc acgggcaggc ggttggcatg ttctcgggcg
     1441 acgagatgct ccacggcacg gaccccgtgc acggcatcga gctctgcgcg gtggtggagt
     1501 tcatgctcag cctcgagacg ctcatgacgg tcacgggtga cgtggccttc gtcgaccggc
     1561 tggagcgcgt cgcctacaac gccctcccga cccaggtcac gggcgaccag gcgggccggc
     1621 agtacttcca gcaggtgaac caggtgcgcc tctcgtcggg cgaccacggc atcttcttcg
     1681 aggggtacga ggacgcgacc tgcttcggcc tcctcaccgg ctacccgtgc tgcaccacga
     1741 acctgcacca gggctggccg aagctcacgc agcacgcgtg gctccggagc gcggacgggg
     1801 gcctcgcggc cttggtctac gcgccgacgt ccgtccgcgc gagggtcggc ggcgccaccg
     1861 tggcgatccg cgaggtcacg tcctggccca tggaggacac cgtgcgcttc caggtcaccg
     1921 aagcggacgc ggtccgcttt cccctgcacc tgcggattcc cgcctgggcc gtcggcgccc
     1981 gcctcacggt gaacggcgag ccgggcggcc gcttcgagcc ggggaccatc gcggtggtcg
     2041 accgcacctg gagcgcgggc gacgaggtgg tcctggcact gcccgcggac atcgaagtgt
     2101 cgcggtggca cgagaacggg atcgccgtgc accgtgggcc gctgcagttc gcgctgccca
     2161 tcgacgcggt catgcgtccc gccctggcga agaacccggg cacgaactcg ctcgagatcc
     2221 tggaggcaca ccccacaggg gaatggaatt acgggctgac ggtggacgcc gggcacccgc
     2281 tccagggctt cgagatcgtc cgcgacccgc ggccggccgc ctggccgtgg accgaggccg
     2341 gcgcgcccat acgcctcagg gccacgggct acccgatccc gagctggcgc gagtacaaca
     2401 gcgcggccgg cccgatgccg ccgagcccgg tccgggcccg gctgggcaca ccccggacga
     2461 tcgagctcgt cccgtacggc gctacgaccc tgcgcatcgc ggtgtttccc gaagtccgtc
     2521 cgcggtgaat cccatgaccg atcctgccgt ggcggtgcag cgcaaattcc tgattgccgt
     2581 cgtcatcgtc gtgacgatcc tgttcctgtg gatggtgcgc tcgttcctga tcgcgatgtt
     2641 catggcagcg atcttcagcg ccatggcccg cccgctgtac cgccggatga cccgctggtg
     2701 ccgaggtcgc acgagcctgg ccgccgggtt gaccctcgtg gtgctgatcg tcgcggtcgg
     2761 cctgccgatg tcgggcttcg tgaccctggt ggtctcccag gcgatcgaca tcaccgaggc
     2821 ggcacgcccc tgggtggagc ggcagatcac ggccccaggc gcctgggacc gcctggtgga
     2881 gcagttcccg ctgctgtccc ggatcgtccc gccggggacc gacgtggccg cgaaactggg
     2941 cgagttcgcg acctcggtcg gcgagttcct ggcgaactcg atggtcgacg tcacgcgcgg
     3001 cacggccgcc ttcttcctgc agctcttcgt gatgctgtac gcgatgtact tcttcctggt
     3061 gggggggcgc gtcaccctgg agcgcatcat cggcgtcatt cccctggacc aggatacctc
     3121 gcgggccctg gtcgcgcagt tcgtctcggt gacccgggcc gtcctcaagg gctcgctcat
     3181 catcggcgtg atccagggcg tgatggccgg gggggccttc tggatcgcgg gcatccaggg
     3241 ctgggccttc tggacgacca tcatgatcgt gatgtcggtg atcccggcca tcgggtcggc
     3301 gctggtgtgg atcccggccg tgatctacct cctgttgaac ggggacgtga tggccggcgt
     3361 cctcctcgcc atctggtgcg ccgcggtcgt gggctcggtg gacaatttcc tcaggccgat
     3421 cctcatcgga cgtgacacga agatgcccga cctgctcgtt ctggtcggca cgctcggcgg
     3481 catcttcctt ttcggtgcgc tgggcttcat cctcgggccc atcgtcgcgg cgctcttcct
     3541 cgcggtctgg gacctgtacg gggcggaggt ccgggaggcg cccccggaac cgcaaaggcc
     3601 atgagaatcg ccctggtggt cctgctgctg gccgcctgcg gctcgcccga gccgcgcccc
     3661 cggatggtgc ccttcgagga accggagtct
//
LOCUS       DBNHQZ010000388         7322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1198236, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000388 DBNHQZ010000000
VERSION     DBNHQZ010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1198236"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="rsgA"
                     /locus_tag="AC16YU_10510"
     CDS             <1..594
                     /gene="rsgA"
                     /locus_tag="AC16YU_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6065699.1"
                     /translation="AGDRDRALAAARELADAVAVSSVAEGGLDALAAVMEAGRTYILL
                     GPSGVGKSTLLNRLAGEDLFATREVRGADQKGRHTTTRRQLVVLPSGALVIDTPGMRE
                     LGMVDVQEGIGETFADLAALAEDCRFRDCTHTTEAGCAVIAAVEAGEVDRGRYESWLR
                     LAREAAHYERDYVEQRRRDRAFGKMVREVLKQKKDRL"
     gene            complement(727..1395)
                     /gene="clpP"
                     /locus_tag="AC16YU_10515"
     CDS             complement(727..1395)
                     /gene="clpP"
                     /locus_tag="AC16YU_10515"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR6065700.1"
                     /translation="MGRLPGDIYSGPIDTGSWGTLVPMVVEQTSRGERAYDIFSRLLK
                     ERIVILGTPINDQIANLMVAQLLFLASEDPERDINIYVNSPGGSIDSGLAVYDTMQYI
                     SCHCATICVGLAASMGSVLLAAGSRGKRATLPNSRIMIHQPWMGGVQGQASDIEIHAR
                     EIIKMKQRLYEILAEHTGQTPAQIEKDGDRDYWMGADEAKEYGLVDNVLLPKPKGVKK
                     KEEE"
     gene            complement(1496..2869)
                     /gene="tig"
                     /locus_tag="AC16YU_10520"
     CDS             complement(1496..2869)
                     /gene="tig"
                     /locus_tag="AC16YU_10520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6065701.1"
                     /translation="MPNTISRISDVEYELRIEASADELAPELRKAIRTQRARTTMKGF
                     RPGRVPESLVEKIYGKALAYGIADEHVQKAYRDEVLEGSAYDVMGSPMITELEYEYGA
                     DLAATVRFGVRPDIELADLSDVLVTRLVHTVTDGEVGEELESIREREAELTTVDGPAE
                     ADSLVTVDLQRLDPESGTPIVGEKREDVSFFVSNEELPESTRKALVGVPAGQTVRIAL
                     VQTENGEEALFDATVKEVKRRDLPELDDEFVTTLTNGEITDVETFRVDLRTRMQRSID
                     RTAKELFEGDLVKAVLDRHDFDVPSSVVDMVLDGRFEDFKRQLGDRMPENFDVEGFRE
                     AGRPEATRQARWMLIRDHVARREGLEVTDEDRDAYFAEMAAGQGMPADFLARYYRSMP
                     KLMQQLDERLQTGKVLDWLAGLVGVEEKDRDTWAAGRDEHVHHEHAHHDHAHHHHDHD
                     HDHDHDH"
     gene            complement(3007..4302)
                     /locus_tag="AC16YU_10525"
     CDS             complement(3007..4302)
                     /locus_tag="AC16YU_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065702.1"
                     /translation="MNGIWIVAKNEFLKRIRSRWFVLTTLLGPLALIAFFAVIGFVSV
                     KAMESGERTVLVIDGTGVLADSLVARSNADLTFRRAEVPADSVRRTVLSGDADGYIDI
                     PASVLAGEGQVTYYSMEGGIGGIFQNRLEWVVERVVADHRLAEQQVSQAVREIFESRT
                     GFRSVKLSKEGEEVGDAGAYAVIGFIMGFLIYIAMLVYGSVVMQGVIEEKLSRVVEII
                     VSSVRPFQLLLGKVLGIGAMGLVQMITWALLIMAVTVFSGTIIALFIDPAQLNLPVDA
                     SQQELLQAVDFQVPTVGAEVFVWFVLFFLVGYLLYASLFSAIGSAVEQQQDAQGLMLP
                     VMMPIIMSIIFIQPIIEAPNSPLAVTLSMIPFFSPIPMVVRVAVTDVPFWQPLLSFAL
                     MVATFLGSVWVSARIYRIGILMYGKKAKLSDLIRWMRYA"
     gene            complement(4299..5243)
                     /locus_tag="AC16YU_10530"
     CDS             complement(4299..5243)
                     /locus_tag="AC16YU_10530"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065703.1"
                     /translation="MPHLQVADISKQFGAVKAVDHVSFEAKPGRILGLLGPNGAGKTT
                     SIRMIAYITVPDSGRVLLDGREVGPWSQRVMGYLPEERGLYKKMKVGEQLTYFGELKG
                     LPRSQAVERTRHWLGRFHAEDWFGRKTGELSKGMQQKVQFIATILHDPSLLILDEPFG
                     GLDPLNADLLEDVLMELKSDGRIILFASHRMEQVEQLCDDICLISDGHIVLDGSLRDV
                     KRSFGRNTVLIAFDGPDTFLDDLEREGRIRVGTRSLGTADLQLLDGTSPRLVLERALS
                     EVSEVSRFELVEPPLREIFVTVVQDQRAAELETSRNRA"
     gene            complement(5307..5579)
                     /locus_tag="AC16YU_10535"
     CDS             complement(5307..5579)
                     /locus_tag="AC16YU_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065704.1"
                     /translation="MKPSAPTYRLEMLPAVPPFHRRARRRKRLDAGQLVYYGPMPRYY
                     GIGEVKHIEDGYVAVDFRGTGEFGVHEDVFEPHYLIPIPASTMDLL"
     gene            5722..6024
                     /locus_tag="AC16YU_10540"
     CDS             5722..6024
                     /locus_tag="AC16YU_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HIR6065705.1"
                     /translation="MTAPTPYRDKIVDRQVGRNAELFAEAIGAMDTAEARYPYLRILI
                     SVLEQAHPEWNQAPTKDRHIAHIVFKMSRGQILMDETAEVVRLRDEERGFSRYDRD"
     gene            complement(6056..6598)
                     /gene="ruvC"
                     /locus_tag="AC16YU_10545"
     CDS             complement(6056..6598)
                     /gene="ruvC"
                     /locus_tag="AC16YU_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6065706.1"
                     /translation="MIILGIDPGSRITGYGVIEVENGRERPIEFGVLRMEEEDDHHLR
                     LRRIHERLTAVIDRCLPDLCAIEMPVYGRNAQSMLKLGRAQAAAMLAALVRDVPVVEY
                     TPAEVKKSVTGNGAATKEQVGYMVRAILELDDQADPMALDASDALAVSLCHAHRIRSS
                     GSGRSSGWKQFVRDNPGRVR"
     gene            complement(6595..>7322)
                     /locus_tag="AC16YU_10550"
     CDS             complement(6595..>7322)
                     /locus_tag="AC16YU_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6065707.1"
                     /translation="SKIKRQKAATDARKSQAWARITRDIMVAAREGGGDPGMNARLAL
                     AVEKAKQENMPKDNVERAIKRGTGEIQGADYEEITYEGYAAGGIAVLVEALTDNTNRT
                     VADLRALFSRAGGSLGQSGSVGFLFERKGQFEVPASAADELTLFDLVADAGAEDLTLD
                     DGSFVITTPVEAFGAVQRALDAKGIEVSSAELIRIPTMTRQVDLATARSVAALIEKLE
                     DYQDVQAVYTTLEFSDELVDALQ"
BASE COUNT         1105 a         2519 c         2491 g         1207 t
ORIGIN      
        1 gcgggcgacc gcgaccgtgc actcgccgcg gcccgcgagc tcgccgacgc cgtcgcggtc
       61 tcgagcgtcg ccgagggagg cctcgacgcg ctcgccgccg tcatggaggc gggacgcacg
      121 tacatcctgc tcggaccctc gggcgtgggc aagtccacgc tcctgaaccg cctcgcgggc
      181 gaggacctct tcgcgacgcg cgaggtgcga ggcgccgacc agaagggccg ccacacgacc
      241 acgcgccggc agctcgtggt cctgccttcg ggcgccctgg tcatcgacac gccgggcatg
      301 cgcgagctgg gcatggtcga cgtgcaggag gggattggcg agaccttcgc ggacctcgcg
      361 gccctggccg aggactgccg gttccgggac tgcacgcaca cgaccgaggc cggctgcgcc
      421 gtcatcgcgg ccgtcgaggc gggcgaggtg gaccggggcc gctacgagag ctggctgcgc
      481 ctcgcgcgcg aggccgccca ctacgagcgc gactacgtgg agcagcggcg gcgggaccgg
      541 gcgttcggga agatggtgcg cgaggtactc aaacagaaga aggaccgcct ttgaggcgcg
      601 cgggtccgcg cgcatctccg cgttggcctt cgtcgccgct cgctgcgacg tgcatcagca
      661 cgactcgctc gcgttcctcg gccgccttga tgtgcacgcg gcctcgcgcg cctgcggcgg
      721 cgggtcctac tcctcctcct tctttttcac gcccttcggc ttcggcagga gcacgttgtc
      781 cacgagcccg tattccttcg cctcgtcggc gcccatccag tagtcgcggt cgccgtcctt
      841 ctcgatctgg gccggcgtct ggccggtgtg ctcggccagg atctcgtaga gccgctgctt
      901 catcttgatg atctcgcggg cgtggatctc gatgtccgac gcctggccct ggacgccgcc
      961 catccacggc tggtggatca tgatgcgcga gttcgggagc gtggcgcgct tgcccctgga
     1021 gccggccgcg agcagcaccg accccatcga cgccgcgagc cccacgcaga tcgtcgcgca
     1081 gtggcacgag atgtactgca tggtgtcgta caccgcgagc cccgagtcga tggatccgcc
     1141 cggcgagttc acgtagatgt tgatgtcgcg ctccgggtcc tcgctcgcga ggaacaggag
     1201 ctgggcgacc atgaggttcg cgatctggtc gttgatcggg gtcccgagga tcacgatccg
     1261 ctccttgagc aggcggctga agatgtcgta ggcccgctcg ccgcgcgagg tctgctcgac
     1321 gaccatcggc accagcgtgc cccacgaccc ggtatcgatc ggaccgctgt agatgtcgcc
     1381 gggcagcctg cccatgcctt tcgcaaaccg ggtaaaggag tcgaccatgt cgggtggcgg
     1441 atggagttcg tgcggtgcag tctaccgggc gcccggaggg cggttccggc ggcgatcagt
     1501 ggtcgtgatc gtggtcgtga tcgtggtcgt gatgatggtg ggcgtggtcg tggtgggcgt
     1561 gctcgtggtg cacgtgctcg tcgcgtccgg ccgcccacgt atcccggtcc ttttcctcga
     1621 cgccgaccag gcccgcgagc cagtccagca ccttgccggt ctgcagcctc tcgtcgagct
     1681 gctgcatgag cttgggcatg gagcggtagt accgggccag gaaatccgcg ggcatgccct
     1741 gcccggcggc catctcggcg aagtacgcgt cgcggtcctc gtccgtgacc tcgaggccct
     1801 cgcggcgcgc gacgtggtcc cggatcagca tccagcgcgc ctggcgcgtg gcctcgggcc
     1861 gccccgcctc gcggaagccc tccacgtcga agttctcggg catgcggtcg cccagctggc
     1921 gcttgaagtc ctcgaaccgt ccgtcgagca ccatgtcgac gacggacgag ggcacgtcga
     1981 aatcgtgccg gtccagcacg gctttcacca ggtccccctc gaagagctcc ttcgccgtcc
     2041 ggtcgatcga gcgctgcatc cgggtgcgca ggtccacgcg gaacgtctcg acgtccgtga
     2101 tctcgccgtt cgtgagcgtc gtaacgaatt cgtcgtcgag ctcgggcagg tcgcgccgct
     2161 tcacctcctt gacggtagcg tcgaagagcg cctcctcgcc gttctcggtc tgcacgaggg
     2221 cgatgcgtac ggtctgcccc gccggaacac cgaccagcgc cttgcgggtg gactcgggca
     2281 gttcctcgtt cgagacgaag aacgagacgt cctcgcgctt ttcgcccacg atcggggtcc
     2341 cggattccgg gtccaggcgc tgcaggtcca cggtcaccag gctgtccgcc tcggccgggc
     2401 cgtcgaccgt cgtgagctcc gcctcgcgct cccgtatgct ctccagctcc tcgcccacct
     2461 cgccgtcggt gaccgtgtgg acgagccggg tcaccaggac gtccgagagg tcggcgagct
     2521 cgatgtccgg ccgcacgccg aagcggacgg ttgccgcgag gtcggctccg tactcgtact
     2581 cgagctcggt gatcatgggc gagcccatga cgtcgtacgc cgagccctcg agcacctcgt
     2641 cgcggtacgc cttctgcacg tgctcgtcgg cgatgccgta ggcgagcgcc ttcccgtaga
     2701 tcttctcgac cagggactcg gggaccctgc ccggccggaa tcccttcatg gtcgtccggg
     2761 cgcgctgcgt ccggatggcc ttccggagct cgggagccag ctcgtcggcc gaggcctcga
     2821 tccggagctc gtactcgacg tcgctgatgc gggagatcgt gttgggcata ggtggtccgc
     2881 ggaggaggag ggagtcggcc ggccgggaaa tcccggcaaa gagcgactca aaccgccacg
     2941 cgggcgggcg gttcttcggc gccgcggacg cggccgcatt tcacggtttc tgtaccgcgg
     3001 ggcgcgtcag gcgtaccgca tccagcggat caggtcggaa agcttcgcct tcttgccgta
     3061 catgagaatg ccgatgcggt agatccgtgc gctcacccag accgagccca ggaacgtggc
     3121 caccatgagc gcgaacgaga ggagcggctg ccagaagggc acgtccgtca cggccacccg
     3181 cacgaccatc gggatgggcg agaagaacgg gatcatggac agcgtcacgg cgagcggcga
     3241 gttgggcgcc tcgatgatcg gctggatgaa gatgatcgac atgatgatgg gcatcatcac
     3301 gggcagcatc aggccctgcg cgtcctgctg ctgctcgacc gccgatccga tggccgaaaa
     3361 gaggctcgcg tagagcaggt agccgaccag gaagaagagc acgaaccaga cgaaaacctc
     3421 ggctccgaca gtcggcacct ggaagtccac ggcctgcagc agctcctgct gggatgcgtc
     3481 gacggggagg ttcagctgcg ccgggtcgat gaagagcgcg atgatcgtgc ccgagaacac
     3541 ggtgacggcc atgatcagga gggcccacgt gatcatctgc acgaggccca tggccccgat
     3601 tccgagcacc ttgccaagga gcagctggaa cggtcgcacc gacgacacga tgatctcgac
     3661 cacacggctg agcttctcct cgatcacgcc ctgcatcacg acggacccgt agaccagcat
     3721 ggcgatgtag atcaggaagc ccatgatgaa cccgatcacc gcgtacgccc cggcgtcccc
     3781 gacttcctcc ccctccttgc tcagcttgac cgagcggaaa ccggtccggc tttcgaagat
     3841 ctcccgcacc gcctgcgaga cctgctgctc ggccagccgg tggtcggcga cgacccgctc
     3901 cacgacccat tccaggcggt tctggaagat gccgccgatg cccccttcca tgctgtagta
     3961 ggtcacttgg ccctcaccgg cgagcaccga cgcggggatg tcgatgtatc cgtcggcgtc
     4021 tccgctgagc accgtccgcc ggaccgaatc cgccggcacc tcggcgcgcc ggaacgtcag
     4081 atccgcgttg cttcgcgcca ccaggctgtc cgccagcacg ccggttccgt cgatgaccag
     4141 cacggtgcgc tcgccgctct ccatggcctt gacggacacg aatccgatga cggcgaagaa
     4201 cgcgatgagc gccagggggc ccaggagggt cgtgaggacg aaccagcggg accgaatccg
     4261 cttcaggaac tcgttcttgg cgacgatcca gatgccgttc atgctcgatt ccggctggtt
     4321 tccagttcgg ccgcccgctg gtcctggacg accgtgacga agatctccct caggggcggc
     4381 tccacgagct cgaagcgcga gacctcgctc acttccgaga gcgcccgctc cagcaccagt
     4441 ctcggggagg ttccgtcgag gagttgcagg tcggccgttc cgagacttcg cgttcccacc
     4501 cggatgcggc cctcgcgctc cagatcgtcc aggaacgtgt ccggtccgtc aaaggcgatc
     4561 agcacggtgt tgcggccgaa gctccgcttc acgtcgcgca gcgatccgtc gagcacgatg
     4621 tggccgtcgg aaatcaggca gatgtcgtcg cagagctgct cgacctgctc catgcggtgc
     4681 gacgcgaaga ggatgatccg gccgtcggac ttgagctcca tgagcacgtc ctcgagcagg
     4741 tcggcgttca acgggtcgag gccgccgaac ggctcgtcca ggatcaggag cgaggggtcg
     4801 tgcaggatcg tcgcgatgaa ctgcaccttc tgctgcatgc ccttcgagag ctcgcccgtc
     4861 ttgcggccga accagtcctc ggcgtggaag cggccgagcc agtgccgggt ccgctcgacg
     4921 gcctgcgacc gcgggaggcc cttgagctcg ccgaagtagg tcagctgctc gccgaccttc
     4981 atcttcttgt agagcccgcg ctcctcgggc aggtagccca tcacgcgctg gctccacggg
     5041 cccacctcgc gcccgtccag gagcacgcgc cccgagtccg gcaccgtgat gtaggcgatc
     5101 atccggatgg aggtcgtctt gccggctccg ttgggtccga gcagccccag gatgcgcccc
     5161 ggtttcgcct cgaacgaaac gtggtccacg gccttcacgg ccccgaactg cttggagatg
     5221 tccgctacct ggagatgggg catgtcatgc gtcgcgcgtc tgccgtcggg cgcaggctac
     5281 ggcacgcggg ccatccggat gcgtcctcac agaagatcca tcgtggaggc cggaatcgga
     5341 atcaggtaat gcggctcgaa cacgtcctcg tgcacgccga actcccccgt gccgcggaag
     5401 tccacggcga cgtagccgtc ctcgatgtgc ttcacctccc cgatgccgta gtaccgcggc
     5461 atcgggccgt agtagacgag ctggcccgcg tccaaccgct tccggcgccg cgccctgcga
     5521 tggaagggcg ggacggcggg cagcatctcc aggcgatatg tcggggcgga cggtttcacg
     5581 gccgtgcggg ctcggccgtt gaacgcgtgc gcgatccggc gggttcgcat gaacccgcgc
     5641 gcgattcatg ggttggaggg gcttcgaagg ctattctccc gacctgcgcc cgacctgcgc
     5701 ccggccgatc ccgcaccccc gatgaccgcc ccgacaccgt accgagacaa gatcgtcgac
     5761 cgccaggtcg gccggaacgc cgagctcttc gccgaggcca tcggagcgat ggacacggcc
     5821 gaggcgcggt atccctacct gcgcatcctg atctcggtcc tggagcaggc ccacccggag
     5881 tggaaccagg cgccgaccaa ggaccggcac atcgcgcaca tcgtcttcaa gatgagccgc
     5941 gggcagatcc tgatggacga gaccgccgag gtggtccggc tgcgcgacga ggagcggggc
     6001 ttcagccggt acgatcgcga ctgaacggga gggcgcccac tgtcagggga ttccgtcagc
     6061 ggacccggcc cgggttgtcc cgcacgaact gcttccagcc cgacgagcgc cccgagccgg
     6121 acgagcggat ccggtgggcg tggcagagcg acacggccag cgcgtccgag gcgtcgagcg
     6181 ccatggggtc ggcctggtcg tcgagctcca ggattgcccg gaccatgtag ccgacctgtt
     6241 ccttggtcgc tgcgccgttg cccgtgaccg acttcttgac ctcggccggc gtgtactcga
     6301 cgaccgggac gtcgcgcacg agcgcggcga gcatggcggc ggcctgggcg cgcccgagct
     6361 tgagcatgga ctgcgcgttt cggccgtaga ccggcatctc gatggcgcac aggtcgggca
     6421 ggcaccggtc gatcaccgcg gtcagccgct cgtggatgcg gcgcagccgc aggtggtggt
     6481 cgtcctcctc ctccatccgc agcaccccga actcgatcgg ccgctcccgc ccgttctcga
     6541 cctcgatcac gccgtagccg gtgatgcggg acccggggtc gatgccgagg atgatcattg
     6601 gagggcgtcg acgagctcgt cgctgaactc gagcgtggtg tagacggcct gcacgtcctg
     6661 gtagtcctcg agtttctcga tgagcgccgc gacggaccgc gccgtcgcga ggtcgacctg
     6721 ccgggtcatg gtcggaatgc ggatgagctc ggcgcttgag acctcgatgc ccttggcgtc
     6781 cagggcgcgc tggaccgcgc cgaaggcttc gacgggcgtc gtgatcacga acgacccgtc
     6841 atccagcgtg aggtcctcgg cccccgcgtc ggccacgagg tcgaagagcg tcagctcgtc
     6901 ggcggccgaa gccggcacct cgaactgccc cttgcgctcg aacaggaagc ccaccgaccc
     6961 ggactggccc agcgagccac cggccctcga gaagagggcc cgcaggtcgg ccaccgtccg
     7021 gttggtgttg tcggtcaggg cctcgaccag gaccgcgatg ccgcccgcgg cgtagccctc
     7081 gtaggtgatc tcttcgtagt cggcgccctg gatctcgccc gtgccgcgct tgatggcgcg
     7141 ctccacgttg tccttgggca tgttctcctg cttcgccttc tcgaccgcga gtgcgaggcg
     7201 ggcgttcatg ccggggtcac cgccgccctc gcgcgcggcg accatgatgt cgcgggtgat
     7261 gcgggcccac gcctgggact tcctggcgtc ggtcgcggcc ttctggcgct tgatcttgga
     7321 cc
//
LOCUS       DBNHQZ010000389        10621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1877301, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000389 DBNHQZ010000000
VERSION     DBNHQZ010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1877301"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC16YU_10555"
     CDS             <1..629
                     /locus_tag="AC16YU_10555"
                     /inference="COORDINATES: protein motif:HMM:NF037527.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baeRF3 domain-containing protein"
                     /protein_id="HIR6065708.1"
                     /translation="ARRGGGQGASVLVPGPGGGGDRANVKRKILEFFRQVAAGVMAAL
                     AGRSDPLVLVGLPHLVGLYREANRYPHLVAEAVLADPEPLGREALRDRALGVLAPRER
                     ERRDELLNQFGTLQSASPERTATHLEDVLGAAATGRVDTLIVARGERAWGRYAGPDRT
                     VVHPVRRSGDEDLVDRAAWYTLRQGGGYRPVEPDMVPGGGPAAAILRY"
     gene            complement(623..2554)
                     /locus_tag="AC16YU_10560"
     CDS             complement(623..2554)
                     /locus_tag="AC16YU_10560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6065709.1"
                     /translation="MTRPAAFALLLLFALTAPGSSAQDRRPIPYPVIPGPGFDRAVEA
                     GTRTTTGHPGPNYWTNTAEYVIEATLSPETSVVRGTGSVTYHNHSPSPLNLVLVHLRQ
                     NLHAEGVVRNRPQRLTGGVTLGEVTVGGEPLAEVPGLRSPGYRVDGTVMTIRPPEPVQ
                     PGESVQLAFEWSYRVPDAGAPRNGTDGEVWYMAYWYPQIAVFDDVNGWKADPYMGEGE
                     FYMGYADYDVRLTVPEGFLVAATGTLQNPDEVLSDQTAGRLEAARNNPEAIIRVVDEE
                     DRKAGVSTASSDSGLLTWHFRAERVRDFAWGTSSRFLWDATVAEVGDHDGDGAPDRAD
                     IFAFWRPEAETWFRSAEFGKFSIEFLSRTFFPYPYPHMTAVEGPMGGGMEYPMMTLIG
                     RSDSDRGLFGVTLHEIAHMYFPMIVGQDEKEDTWMDEGLTSFNTAEGSAEYWGDDPWS
                     GERQGYFSIAGSGQEVESRRHADRYPLRTSARGIAAYSKPAVALHALRGLLGNDLFYR
                     AYREYATRWSWRHPQPEDLFNTFEDVVGRDLDWFWTTMFYTTWTLDQGIASVTEGQDG
                     VRIQIEDHGLSPFPAPVRVTYADGTTAEAWIAVEPWLQGERTQTLRAPAGSVTRVEID
                     PEQFLPDVNRSNNLWKAAQ"
     gene            2680..3720
                     /locus_tag="AC16YU_10565"
     CDS             2680..3720
                     /locus_tag="AC16YU_10565"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR6065710.1"
                     /translation="MKLSLRWYGPDDAVRLADMAQIPDLTGIVTALHGIPPGLAWSRE
                     AIDRRHEEVQAAGLRWVVAESIPVPEPVKLGTDGRDAAIEAWCRSLEALGASGVPVVC
                     YNFMPVFDWMRTDLAERAADGSFSPAFREADLSRFDLDRGFDALPAWAESYAPARLLE
                     LRDSYARLDAERIWDNLGYFLERVVPVAESAGVRLALHPDDPPWPVFGIPRIITDGAS
                     LRRVLELDDRKANGLTFCTGSLGARSDCDLPALVRELGSRIHFAHCRNVRRLGPRHFV
                     ETAHSRSAGDVDLPAVLRAYRETGFEGPMRPDHGRMIWGESGRPAYGLYDRALGASYL
                     SGAWHALGGSLS"
     gene            complement(3737..4180)
                     /locus_tag="AC16YU_10570"
     CDS             complement(3737..4180)
                     /locus_tag="AC16YU_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065711.1"
                     /translation="MQPINRTAVVRGGLVAGIVLILMEAVLMTFLQPTWIDLVRNMGG
                     RPPDLFAAACYAVVLVVVGIVIVWFYAAIRPRYGPGPRTAVRAGAAVWIMGWGAGFGS
                     AAVLGIFPAGFVVIVLGWGLIECLLAALAGAIVYTERPPAGAPEG"
     gene            complement(4222..5073)
                     /locus_tag="AC16YU_10575"
     CDS             complement(4222..5073)
                     /locus_tag="AC16YU_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6065712.1"
                     /translation="MPTSRRSFIRSAAVAAAALPVGLPAASRAARRRGGRVRLGVASY
                     SLRGLDREAAIRVLVALRTPYVSIKSFHLPYESTPAEIEAGRRAFLDADLTIVGGGLV
                     PLTEDDDASMTRYFEYAKAAGFPMMVIGTTPDALPRVERFVKTFGIAVALHNHGPEDP
                     HFPAPSDALPLIRDMDPRVGLCIDVGHAARTGRDVVQEIADAGDRVLDVHLKDLADLS
                     DAASQCVVGEGSMPIPDIFRQLDRMGYGGCANLEYEIDESDPAPGMLRSFAYMRGVLA
                     GLGLSDR"
     gene            complement(5086..6417)
                     /locus_tag="AC16YU_10580"
     CDS             complement(5086..6417)
                     /locus_tag="AC16YU_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065713.1"
                     /translation="MDRRRFIRQTTAAGVGLGLSGQWPGILRTRRPAETVRVAVMGVY
                     GRGGVLASEFARLPDCRVAVICDVDRRAAERVAASLVEGPEPLAPERPRIEPDIRRVL
                     EDPSVDAVAIAAPDHWHAPAAIMAMRAGKAVYVEKPCGTTPAEGEILVAVQRETGRVV
                     QMGNQQRSAPPSIEVIGMIRDGLIGRPYLARTWYANTRGSIGRGAEATVPDWLDYELW
                     QGPAPRTPYRDNVIHYNWHWFHRWGTGEICNNGTHEIDVARWALGVDFPTRVTSSGGR
                     YHFDDDWEFADTQVTTFEFGDRSQITWEGRSCNGLPIHSRGRGTAVHGETGTVIVDRS
                     GYVAYDLDNREIARRIVGESADALDTRGSSDLTDAHVANFLGAIRGTDTLTAPIDDAA
                     RSVLLCHLGNIAQDTGRALRCDPSDGRIVNDPDAMARWERTYEPGWEISSL"
     gene            complement(6498..7511)
                     /locus_tag="AC16YU_10585"
     CDS             complement(6498..7511)
                     /locus_tag="AC16YU_10585"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HIR6065714.1"
                     /translation="MNPPFPVTEATEAFIRALPKTETHLHLEGALPFALLSEAAPDAY
                     ATPPESWRWDFRFPDFTTFERGLIRMASSYYTSPERYHESATRVFRTLALEQNVRYVE
                     TSFASGLVEHLGLDGAAVAEAIRSAAPESLEVRVFMGIHHKGYHDGTAAWIDACTSWK
                     HLDGIDLHGPETDPLGPWAARLWREAREAGLMTKAHAGEFDGPAFVRRAVDELGVSRI
                     EHGVRCLEDPDLVQELAGRGVTLDVCPISNVKLGVVPSYESHPLGALLDAGIPCTVST
                     DDPLVFGNTLFDEYALLASRLGFGHADLARVAANGFRVARVDKAWADARIREIEAVPG
                     GAV"
     gene            7678..8091
                     /locus_tag="AC16YU_10590"
     CDS             7678..8091
                     /locus_tag="AC16YU_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065715.1"
                     /translation="MATKIRRVEYFNTAVRDEPGSAYRILADIAGQGVGLLAFTAVPV
                     GPTLTQLTLFPDDPGRLWETASLARIHLDGPYPALLVQGDDQLGVLADLHEKLYQAGV
                     NVYASNAVTDGKGDFGYIIYVRPDKFDAAARALGI"
     gene            complement(8163..9608)
                     /locus_tag="AC16YU_10595"
     CDS             complement(8163..9608)
                     /locus_tag="AC16YU_10595"
                     /inference="COORDINATES: protein motif:HMM:NF015563.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR6065716.1"
                     /translation="MSRSLLDRLKVPHVFTLLTAVVLACSLLTWVVPSGEYQRETIQV
                     GQMERNVIVPESYGRIPKHYSLKGAVFADPADGMASPVSAEGFLSAIPRGMEAAADII
                     FFIFVIGGVFGILQRTGTIVAGIHRLLARFRNSGPILIIVLMTAIAIGGSTLGMGEEF
                     IPLVPLFLIVSKELGYDRVFGLALVFAAAETGFAAATTNPFTVNIAQGIAELPLNSEI
                     GFRLVFFVCILTVLVTYTLRYGARVKLDRRNSVMPDDPFDIDTGGADVETFSSRHRMI
                     LVACVTIFGFILWAVQARGWWMAEMGGGFILMGLVAIVVARLPLTEAAKAAARGMEDM
                     VVAALVVGFARAVQVVLDDGQVLDTVIYYAASGLENFSTYTAAAGMLVFQTVLNFFIP
                     SGSGQAAVTMPIMAPLADVLGLTRQTAVFAFTCGDGFSNSIIPTSGVLMAMLGLAGIP
                     YEKWLRFMVPLFLRLMAVAFVFLMIAVAIDL"
     gene            9811..10572
                     /locus_tag="AC16YU_10600"
     CDS             9811..10572
                     /locus_tag="AC16YU_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065717.1"
                     /translation="MHRILRFLSPLILLAALSVSGCDLFGSDDTARVTLNSVADTSPV
                     AKATAAKSASGHVVIDSVKVLFKSIRFHTDDDVVEGEGEVPVEGEEEGGEGSATFHSA
                     PIVVELLVDGSPNEIEVAEIPGGTYDKISFHLHKPRGNQEAEEFPDFAGETPNERWSV
                     VVWGLFEDEFFEYKSALNVVQVVDFDEPFDVEDGASHSATLQVDLSTWFVSLGGVDLA
                     PTDTSEQNRAMIDQSIRNSFHLFQDEDEDGEADAE"
BASE COUNT         1623 a         3601 c         3813 g         1584 t
ORIGIN      
        1 ccgcccggcg tggtggcggg cagggcgcct ccgtgctggt tccggggccg gggggagggg
       61 gggaccgcgc caacgtcaag cgcaagatcc tggagttctt ccggcaggtg gccgcgggcg
      121 tgatggccgc gctggccggg cgctcggacc cgctcgtgct ggtggggctt ccgcacctgg
      181 tgggcctgta ccgggaggcg aaccggtatc cgcacctggt cgccgaggcc gtccttgcgg
      241 acccggagcc gctgggccgg gaggcgctcc gcgaccgggc cctgggcgtg ctcgcaccgc
      301 gcgagcgcga gcgccgggac gagctgctca accagttcgg cacgctgcag tccgccagcc
      361 cggagcgcac ggccacccat ctcgaggacg tgctcggcgc ggcggcgacc ggccgcgtgg
      421 ataccctgat cgtggcccgc ggagagcggg cgtggggtcg ttacgccggc ccggaccgga
      481 ccgtggtgca tccggtccga cgctcgggtg acgaggacct ggtggaccgc gccgcctggt
      541 acacgctgcg gcagggggga ggctaccggc cggtggagcc cgacatggtg cccgggggtg
      601 gtcccgcggc cgcgatcctg cgctactgag ccgccttcca gaggttgttc gagcggttga
      661 cgtccggcag gaactgctcc gggtcgatct cgacgcgcgt gaccgatccg gcaggagccc
      721 gaagcgtctg ggtccgctcg ccctggagcc acggctcgac ggcgatccac gcctccgcgg
      781 tcgttccgtc cgcgtaggtc acccggacgg gcgcggggaa cggcgagagc ccgtggtcct
      841 cgatttggat ccggacgccg tcctggccct cggtcaccga cgcgatcccc tggtccaggg
      901 tccaggtcgt gtagaacatc gtcgtccaga accagtccag gtcgcgtccg accacgtcct
      961 cgaacgtgtt gaagaggtcc tccggctggg gatgtcgcca ggaccagcgg gtggcgtact
     1021 cccggtacgc ccggtagaac aggtcgttgc ccagcagccc gcgaagcgcg tgaagggcca
     1081 cggcgggctt gctgtacgcg gcgattccgc gggccgaggt gcgcagcgga tagcggtccg
     1141 cgtggcggcg cgattccacc tcctgcccgg acccggcgat gctgaaatag ccctgccgct
     1201 cgcccgacca cggatcgtcg ccccagtact cggccgagcc ctccgccgtg ttgaaggagg
     1261 tgaggccctc gtccatccac gtgtcttcct tctcgtcctg tcccacgatc atcgggaagt
     1321 acatgtgggc gatctcgtgg agcgtcacgc cgaagagccc gcggtcgctg tccgaccgtc
     1381 cgatgagggt catcatcggg tactccatcc cgccgcccat cggcccctcg acggccgtca
     1441 tgtgcggata cgggtagggg aagaacgtgc gcgagaggaa ctcgatcgag aacttgccga
     1501 actcggccga ccggaaccag gtctcggcct cgggccgcca gaacgcgaag atgtccgccc
     1561 ggtccggggc gccgtcaccg tcgtggtctc cgacctcggc gacggtcgcg tcccacagga
     1621 accgggacga cgtcccccac gcgaaatcgc ggaccctctc ggcgcggaag tgccaggtca
     1681 gcaggccgga gtcactcgat gccgtggaca cgccggcctt ccggtcctcc tcgtccacca
     1741 cgcggatgat ggcctcgggg ttgttccgcg ccgcctcgag ccgccccgcg gtctggtccg
     1801 agaggacctc gtccgggttc tggagcgtcc ccgtcgcggc gacgaggaat ccctcgggca
     1861 cggtcaggcg gacgtcgtaa tccgcgtatc ccatgtagaa ttccccctcg cccatgtacg
     1921 gatcggcctt ccagccgttc acgtcgtcga agacggcaat ctgcgggtac cagtaggcca
     1981 tgtaccagac ttccccgtcg gtgccgttgc ggggcgcgcc ggcgtccggc acgcggtagc
     2041 tccattcgaa cgcgagctgc acgctctcgc ccggctggac cggttcgggc ggacggatcg
     2101 tcatgacggt gccgtccact cggtatccgg gcgagcgcag gcccgggacc tcggcgagcg
     2161 gctcgccgcc gacggtgact tccccgagcg tcaccccgcc ggtcaggcgc tggggacggt
     2221 tccgcacgac gccctcggca tgcaggttct gacgcaggtg caccaggacc aggttgagcg
     2281 gtgagggcga gtggttgtga tacgtgaccg atccggtgcc ccgcacgacg gaggtctccg
     2341 gcgagagcgt ggcctcgatc acgtactcgg ccgtgttggt ccagtagttc ggcccgggat
     2401 gcccggtcgt ggtccgggtg ccggcctcga ccgcccgatc gaacccgggg ccggggatca
     2461 cggggtaggg aatcgggcgc cggtcctgcg cgctcgatcc cggcgcggtg agggcgaaga
     2521 ggagaaggag ggcgaaagcg gcaggacgag tcatcacgga ggtcgttgaa gatgcggctc
     2581 gaaaggcggg aaggtagggg gatgcggcaa gaaagtcgcc cgcccccgct atcttccgcc
     2641 gcggatcccg acgcgggtcc ggcagacccg attgccacca tgaagctcag cctgcgctgg
     2701 tacggtccgg acgacgcggt ccgcctggcc gacatggccc agatccccga ccttaccgga
     2761 atcgtcacgg cgcttcacgg gatcccgccg ggcctggcat ggagccggga ggccatcgac
     2821 cgcaggcacg aggaggtgca ggccgcgggg ctccggtggg tggtcgccga gagcattccg
     2881 gtgccggagc cggtgaagct cggcaccgac ggccgcgacg cggccatcga ggcgtggtgt
     2941 cggagcctgg aggcgctggg ggcgagcggg gtcccggtcg tgtgctacaa cttcatgccc
     3001 gtcttcgact ggatgcggac cgacctggcc gagcgggccg ccgacggctc cttctcgccg
     3061 gccttccgcg aggccgacct ctcgcggttc gacctggacc ggggattcga cgccctgccc
     3121 gcctgggcgg agtcgtacgc cccggcgcgc ctgctggagc tcagggactc gtacgcgcgc
     3181 ctggacgcgg agcggatctg ggacaacctc ggctacttcc tggagcgggt ggtgcccgtc
     3241 gcggagtcgg ccggggtgcg cctcgcgctg caccccgacg atccgccgtg gccggtcttc
     3301 ggcattccgc gcatcatcac cgacggggcg tccctgcgcc gcgtgctgga gctcgacgac
     3361 cgaaaggcca acggcctgac gttctgcacg ggctcgctcg gggcgcggag cgattgcgac
     3421 cttcctgccc tggtgcgcga gctcggaagt cgcatccact tcgcgcattg ccggaatgtc
     3481 cgccgcctgg gcccgcggca tttcgtcgag acggcgcatt cccggtcggc gggcgacgtg
     3541 gatcttcccg cggtcctgcg ggcgtaccgc gagacgggat tcgaaggccc catgcggccc
     3601 gaccacggcc ggatgatctg gggagagtcc gggcggccgg cgtacggcct ctacgaccgg
     3661 gcgctcggcg cctcgtacct ctccggcgcc tggcacgccc tcggtggctc gctctcctga
     3721 acggccggcg agcggctcag ccctccggcg cacccgcggg cgggcgctcc gtgtacacga
     3781 tggctccggc gagcgcggcc agcaggcact cgatcaggcc ccatcccagg acaatcacca
     3841 cgaagcccgc cgggaagatg cccagaacgg ccgcggagcc gaagccggcc ccccatccca
     3901 tgatccagac cgccgccccc gcgcgcactg ccgtcctggg accagggcca tacctcgggc
     3961 ggatcgccgc gtagaaccag acgatgacga tgccaacgac caccagcacc accgcgtagc
     4021 aggcggcggc gaacaggtcg ggcggccggc cgcccatgtt ccggacgaga tcgatccagg
     4081 tcggctgcag gaaggtcatc agcacggctt ccatgaggat caggacgatg cctgccacga
     4141 ggcctccgcg aacgacggct gtccggttga tcggttgcat gtattccagg gcttggggtt
     4201 ggagtgccgt cgatccgcag gttaccggtc cgagaggccg agacctgcca ggactccgcg
     4261 catgtaggcg aacgagcgga gcatgccggg agcgggatcg gactcgtcga tctcgtactc
     4321 gaggttggcg cagccgccgt agcccatccg gtccagctgg cggaagatgt cggggatcgg
     4381 catcgagccc tcgcccacga cgcactggct ggcggcgtcg gacaggtcgg cgagatcctt
     4441 caggtggacg tcgagcacgc ggtctccggc gtcggcgatc tcctggacca cgtcccggcc
     4501 ggtccgcgcg gcatgcccga cgtcgatgca gagcccgacc cgcgggtcca tgtcccggat
     4561 gagggggagg gcgtccgacg gggccgggaa gtggggatcc tccggaccgt ggttgtgcag
     4621 ggcgaccgcg atgccaaagg tcttaacgaa ccgctccacg cgcggcaggg catcgggcgt
     4681 ggtgccgatc accatcatcg ggaatccggc cgccttcgca tactcgaagt agcgcgtcat
     4741 cgacgcgtcg tcgtcctcgg tgagcggaac caggcccccg cccacgatcg tcaggtcggc
     4801 gtcgaggaac gcccggcggc ccgcctcgat ttccgccggg gtcgattcgt agggcaggtg
     4861 gaaggacttg atgctcacgt agggcgtgcg aagcgccacg aggacccgga tcgccgcctc
     4921 gcggtcgagc ccgcgcaggg agtagctcgc gacgcccagg cggacacgtc ctccccggcg
     4981 ccgggccgcg cgggaagcgg ccgggagacc gaccgggagg gcggccgcgg ccacggcggc
     5041 gctgcggatg aagctccttc ggctggtcgg catgtgggtg ctgggctaga ggctcgagat
     5101 ttcccagccg ggctcgtacg ttcgttccca gcgcgccatc gcgtcgggat cgttcacgat
     5161 ccggccgtcg gagggatcgc agcgcagggc gcgacccgtg tcctgcgcga tgttgcccag
     5221 gtggcagagc aggacggagc gggcggcgtc gtcgatgggg gcggtcaggg tgtcggtgcc
     5281 ccggatggcg cccaggaagt tggcgacatg ggcgtcggtg aggtcgctgc ttccgcgcgt
     5341 gtccagggcg tctgcggact cgcccacgat gcggcgggcg atctcccggt tgtccaggtc
     5401 gtacgcgacg tagcccgagc ggtccacgat cacggtgccc gtctcgccgt ggaccgccgt
     5461 gccgcggccc cgggaatgga tgggcaggcc gttgcagctt cgcccctccc aggtgatctg
     5521 cgaccggtcg ccgaactcga acgtcgtgac ctgggtgtcc gcgaattccc agtcgtcgtc
     5581 gaagtggtac cggccgccgc tcgacgtcac gcgggtcggg aagtccacgc ccagggccca
     5641 ccgcgcgacg tcgatctcgt gggttccgtt gttgcagatt tccccggttc cccagcggtg
     5701 gaaccagtgc cagttgtagt ggatgacgtt gtcgcggtac ggcgtgcggg gcgcgggacc
     5761 ctgccagagc tcgtagtcga gccagtcggg gaccgtggcc tcggcgccgc ggccgatgga
     5821 gccgcgcgtg ttcgcgtacc aggtgcgggc caggtagggt cgcccgatga gcccgtcccg
     5881 gatcatgccg atcacctcga tggacggggg ggcggaccgc tgctggttgc ccatctgcac
     5941 gacccggccc gtctcgcgct gcacggcgac caggatctcg ccctcggcgg gcgtcgtgcc
     6001 gcacggcttc tcgacgtaga ccgccttgcc cgcccgcatg gccatgatgg ccgccggggc
     6061 gtgccagtgg tccggggccg cgatcgcgac ggcatcgacg gaggggtcct cgaggacgcg
     6121 gcggatgtcc ggctcgatgc gcggccgttc gggcgcaagg ggctcgggtc cctcgaccag
     6181 cgacgccgcg acacgctccg cggcccggcg gtccacgtcg cagatgacgg cgacccggca
     6241 gtccggcagg cgggcgaact cgctcgcgag cacgccgccc cgaccataca ctcccatgac
     6301 ggcgacccgg accgtctcgg cgggacggcg cgtgcgcagg atgccgggcc actgcccgga
     6361 caggccgagc cccacgcccg cggccgtggt ctgacggatg aaacgacgtc gatccatggt
     6421 gcgctcgcgt tcgggggacg gagccatgaa tctagcatcg cgccgggtaa ggcgctcggc
     6481 ggcccgcgga ttcccgctca gaccgccccg ccgggcacgg cctcgatctc gcggatccgg
     6541 gcgtcggccc acgccttgtc cacccgggcc acgcggaatc cgtttgccgc cacgcgcgcc
     6601 aggtccgcat ggccgaagcc gagtcgggac gcgagcagcg cgtactcgtc gaagagcgta
     6661 ttgccgaaaa ccagtggatc gtccgtgctc accgtgcacg gaatgccggc gtccaggagc
     6721 gcgccgagcg ggtggctctc gtacgacggc acgacgccga gcttcacgtt gctgatcggg
     6781 cagacgtcga gcgtgacacc ccggccggcg agctcctgga ccaggtccgg gtcctcgagg
     6841 caccgcacgc cgtgctcgat gcgggaaacc ccgagttcgt ccacggcgcg gcgaacgaac
     6901 gcgggtccgt cgaactcgcc ggcatgggcc ttcgtcatca ggcccgcctc gcgcgcctcg
     6961 cgccacagcc gggccgccca gggcccgagc ggatcggtct ccgggccgtg caggtcgata
     7021 ccgtcgaggt gcttccagga ggtgcaggca tcgatccacg ccgcggttcc gtcgtggtat
     7081 cccttgtggt ggatccccat gaataccctc acctcgagtg attccggagc agcgctccgg
     7141 atggcctcgg ccacggcggc tccgtcgagc ccgaggtgct cgaccagccc ggaggcgaag
     7201 ctcgtctcga cgtagcggac gttctgctcg agggcgagtg tccggaatac ccgcgtcgcg
     7261 gactcgtggt accgctcggg cgacgtgtag tacgaggagg ccatgcggat gaggccccgc
     7321 tcgaacgtcg tgaaatcggg gaagcggaaa tcccagcgcc aggactcggg cggcgtggcg
     7381 taggcgtccg gcgccgcctc gcttagaagg gcaaacggga gggcaccttc caggtgaaga
     7441 tgcgtctcgg tcttcgggag ggcccggatg aaggcttcgg tggcctcggt gaccggaaag
     7501 ggtggattca tgtcagccct ggtggtacgc gaattggggc gctccccccg tctcttttcc
     7561 gcagccggac gcttatatag cagtccgggc gtccggtccg ggacgggcct cgcgcagggt
     7621 gtcccgtcgc cggcccgacc gcccgcactc gacacgatga acggacggag gtgcgccatg
     7681 gcaacgaaga ttcgccgcgt ggagtatttc aatacggctg tccgggacga gccggggtcg
     7741 gcgtacagga tcctggcgga tatcgcgggc cagggggttg gcctgctggc cttcacggcc
     7801 gttccggtcg gtcccacgct cacgcagctc acgctgtttc cggacgaccc gggccggctg
     7861 tgggagaccg cgagcctggc gaggattcac ctggacgggc cgtacccggc gctcctggtg
     7921 cagggcgacg accagctggg cgttctggcc gatctgcacg agaagctgta ccaggccggg
     7981 gtgaacgtat acgcctccaa cgcggtgacg gacgggaagg gggacttcgg gtacatcatc
     8041 tacgtgcgtc cagacaagtt cgacgcggcc gcccgggccc tggggatctg aaagcggtgg
     8101 ccggtcttcg ccccggcctc gatgatcccc ggcgcgcggc cgtccgtccg aaggcgccgc
     8161 gactacaggt cgatcgcgac cgcgatcatc aggaagacga acgccacggc catgagccgc
     8221 aggaagagcg gcaccatgaa ccggagccac ttctcgtagg gtatcccggc caggccgagc
     8281 atcgccatca ggacacccga cgtcgggatg atcgaattcg agaagccgtc accgcaggtg
     8341 aaggcgaaaa ccgccgtctg gcgcgtgagg cccagcacgt cggcgagcgg cgccatgatg
     8401 ggcatggtca cggccgcctg gcccgagccc gaggggatga agaagttcag gacggtctga
     8461 aagacgagca tgccggccgc tgccgtgtag gtcgagaagt tctcgagccc cgaggcagcg
     8521 taatagatga cggtgtccag gacctggccg tcgtcgagca cgacctgcac ggcgcgggcg
     8581 aagccgacga cgagcgcggc gacgaccatg tcctccatgc ccctggccgc ggccttcgcc
     8641 gcctcggtca gcggcaggcg cgcgacgacg atcgcgacga ggcccatgag gatgaatccg
     8701 ccgcccatct cggccatcca ccatcccctc gcctggacgg cccacaggat gaacccgaaa
     8761 atggtcacgc aggcgacgag gatcatccgg tgccggctgc tgaaggtctc cacgtcggct
     8821 ccgcccgtat cgatgtcgaa gggatcgtcc ggcatcaccg agttcctccg gtcgagcttc
     8881 acgcgggcgc cgtatcgcag cgtgtaggtc accagcaccg tgagtatgca cacgaagaac
     8941 acgagccgaa acccgatctc gctgttgagc ggcagctcgg cgatgccctg cgcgatgttg
     9001 accgtgaagg ggttggtcgt ggcggccgcg aatccggtct cggccgccgc gaacaccagg
     9061 gcgagtccga acacccggtc ataaccgagc tccttcgaca cgatcaggaa gaggggaacg
     9121 agcgggatga actcctcgcc cataccgagc gtggagccgc cgatggcgat cgccgtcatc
     9181 agcacgatga tgaggatcgg gcccgagttc cggaagcggg cgagcagccg gtgaatgccg
     9241 gcgacgatgg taccggtgcg ctgcaggatc ccgaacacgc cgccgatcac gaagatgaag
     9301 aagatgatgt cggcggccgc ctccatgccc cgcgggatgg cgctcaggaa gccctcggcc
     9361 gacacggggc tcgccattcc gtcggccgga tcggcgaaaa cggcgccctt gagcgagtag
     9421 tgcttcggga tgcggccgta gctctccgga acgatcacgt tccgctccat ctggccgacc
     9481 tggatcgtct cgcgctggta ctcgcccgac ggcacgaccc acgtcaggag gctgcacgcg
     9541 aggacgacgg cggtgagcag cgtgaagacg tgcgggacct tcagccggtc gagcagggat
     9601 ctggacacgg gtcgtggcgg tcggatgggc gaggcgaaga tagcgcccag cgctccgtcg
     9661 gggcaccgcg gcatccgatt ccgggcggcg ggtaggggat tcgcgggccc ggcgtgtact
     9721 tatacgggga atgcccgcgt ctgcttaaac cgggactccc gggggccgat gcccccattg
     9781 atcgctccag acaacaaccc gtcactccat atgcaccgca ttctccgttt cctgtcaccc
     9841 ctcatcctgc tcgccgccct ctccgtctcg ggctgcgacc tgttcggctc cgacgacacc
     9901 gcgcgcgtca cgctgaactc cgtggccgat acgtcgccgg tggccaaggc caccgccgcg
     9961 aagtcggcct ccgggcacgt cgtgatcgac tcggtgaagg tcctcttcaa gtcgatccgg
    10021 ttccacacgg acgacgacgt ggtcgaaggt gagggcgagg tgcccgtcga gggcgaggag
    10081 gagggtggcg agggatccgc cacgttccac agcgcgccga tcgtcgtcga gctcctcgtc
    10141 gacggcagcc cgaacgagat cgaggtggcc gaaattccgg gcgggacgta cgacaagatc
    10201 tcgttccacc tgcacaagcc ccggggcaac caggaggccg aggagtttcc cgatttcgcc
    10261 ggcgagactc cgaacgagcg ctggtccgtg gtcgtgtggg gcctgttcga ggacgagttc
    10321 ttcgagtaca agtccgccct gaacgtggtc caggtggtgg acttcgacga gcccttcgac
    10381 gtggaggacg gagcctccca ttccgcaacg ctccaggtgg atctttccac gtggttcgtg
    10441 agcctgggtg gcgtcgatct cgccccgacc gacacgagcg agcagaaccg ggccatgatc
    10501 gaccagtcga tccggaactc gttccacctg ttccaggacg aggacgagga cggagaagcc
    10561 gacgccgagt agctccagga gcgggcgccg aggcccaatt ttcgcagcaa cttgacgctg
    10621 c
//
LOCUS       DBNHQZ010000390         5383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3503073, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000390 DBNHQZ010000000
VERSION     DBNHQZ010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3503073"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="AC16YU_10605"
     CDS             <1..2379
                     /locus_tag="AC16YU_10605"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HIR6065718.1"
                     /translation="TYTRKVGIEFMHISDPGEKRWLMDRIEPVRGMEPIADATKRRML
                     EKLNAAEAFETFLHTKFIGHKRYSLEGSETLIPMIDMLVSDAADQEVTEVVIGMAHRG
                     RLNLLANILNKPYEVIFSEFEGSIDPTTMQGSGDVKYHLGARGEHQSPSGATVKLTLA
                     SNPSHLEAVDPVVEGMVRARQETLREASASAPGGDYLDAVVPILIHGDAAFAGQGVVA
                     ETLHMSQLPGYRTGGTIHIVVNNQIGFTTGPAEARSSEYASDIARMIQAPIFHVNGDD
                     PEACTRVARLALDFRQVFNKDVVIDMLCYRVRGHNEGDEPTYTQPVLYRKIEEKRSVR
                     KLYTELLLRRGDMEPDEAERMLEDYRQRLQDAFERTKELAERPEPPAEHLLVKPRSER
                     LPDVVTSVDVAAVDRVIQALQTFPEWFSLHPKLARQFERRHRLFHEEKRIDWGFAEAL
                     AFGTTLDEGIRVRVSGQDSRRGTFSHRHAAVYDQESGAEYIPLNHIRDGQAHLYVYDS
                     LLSEYAAVGFEYGYSVSVENALVIWEAQFGDFANGAQIVFDQFLAAAEEKWGQKSGLV
                     LLLPHGYEGQGPEHSSARLERFLQLCAEGNMIVANLTTPANMFHGLRRQVHSTVRKPL
                     VIMSPKSLLRHPLAVSVPEDFTEGGFRTLIPADVPDPAAVDRVVFCSGKVYYDTLQAL
                     QKDEARAGRVALVRVEQLYPFPKHEIADELDRFAGVEDVVWLQEEPANMGAWSFVRWR
                     FDEILEERVGDRTHRIRYVGRPASASPATGSTHVHEREQAALIARAIGAASQ"
     gene            complement(2484..2777)
                     /gene="rpsU"
                     /locus_tag="AC16YU_10610"
     CDS             complement(2484..2777)
                     /gene="rpsU"
                     /locus_tag="AC16YU_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6065719.1"
                     /translation="MPRATRHADSRVARAETPPYLSFLRPSAIYRYPVSVGIKVRDNE
                     SIDRALRRFKRAVNRSRVLRIFRANMAYTKPSEERRVAREKAARNARKRGRYY"
     gene            2816..3403
                     /gene="rdgB"
                     /locus_tag="AC16YU_10615"
     CDS             2816..3403
                     /gene="rdgB"
                     /locus_tag="AC16YU_10615"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6065720.1"
                     /translation="MRLLVATRNPGKLAEFRQLLGDLPVVVVQIDEVDGAPDVVEDRP
                     TLEGNARKKAQTLADFSGLPTLADDTGLEVDALGGRPGVFSARYAGPEEDPAANRRLL
                     LAELAGRPDRSARFRAVIAFAEGGSCRLFEGICEGRIAETPRGEGGFGYDPLFVPGGE
                     DRTFAELDPQAKNAISHRGRALRLFSEYLHERLAR"
     gene            3405..3854
                     /gene="dtd"
                     /locus_tag="AC16YU_10620"
     CDS             3405..3854
                     /gene="dtd"
                     /locus_tag="AC16YU_10620"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6065721.1"
                     /translation="MVALVQRVSEASVAIDGREVASVGRGLLVLLGVHRTDTGAEASW
                     LARKVANLRIFPDADGLMNLSGSDTGAEALVVSQFTLYGNTDKGNRPSFVDSAEPALA
                     EPLYRAFARDLGEHLGRTVPTGEFGAVMQVALVNDGPVTLWVERRAS"
     gene            3851..4873
                     /gene="moaA"
                     /locus_tag="AC16YU_10625"
     CDS             3851..4873
                     /gene="moaA"
                     /locus_tag="AC16YU_10625"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6065722.1"
                     /translation="MTAPGPDITRNGHGMLTDSFGRFHDYLRFSLTERCNLRCTYCMP
                     PEGVELKPRSDILTFEEIERLSRLFVAEGVRKIRLTGGEPLVRRHVEELVDRLGAIEG
                     LESLAVTTNGLLLEPLLPRLQAGGITEINVSLDTLREDRFQQITRRPGLERVRSAIDA
                     AIGAGYRPVKVNCVLMRGVNDDEIGDFVRWTLDRPVSVRFIEYMPFEGNSWDDTRMVP
                     DAEVLDRILAEHPGLVRLDDSPTSTSRTYRVPGAVGTVGLVSSMTAAFCPGCNRLRIT
                     ADGRLMVCLFGRAGVSLRDAMRSGDDDDALRRVVRSAVLGKKAAHAGMEVLAAQTDRP
                     MILIGG"
     gene            4965..>5383
                     /gene="moaC"
                     /locus_tag="AC16YU_10630"
     CDS             4965..>5383
                     /gene="moaC"
                     /locus_tag="AC16YU_10630"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6065723.1"
                     /translation="MTESPSLTHIDPHGGVHMVDISDKDDTVRTAVAAGRIVVGDEAF
                     GMIRSRNVSKGDVLLTAQIAGILGAKQTSRLIPLCHEVSLRGVDVELTLADDAPAIDI
                     RAFAKSVGPTGVEMEALTAVSVAALTIYDMCKSVSKA"
BASE COUNT          848 a         1889 c         1826 g          820 t
ORIGIN      
        1 acctacacca ggaaggtcgg gatcgagttc atgcacatct cggatccggg ggagaagcgc
       61 tggctcatgg accgcatcga gccggttcgc ggcatggagc cgatcgcgga cgccaccaag
      121 cggcgcatgc tcgagaagct gaacgccgcc gaggccttcg agaccttcct gcacaccaag
      181 ttcatcgggc acaagcgcta ctcgctggag ggatccgaga cgctcatccc gatgatcgac
      241 atgctggtgt cggacgccgc cgaccaggag gtgaccgagg tggtgatcgg catggcccac
      301 cgcgggcggc tgaaccttct ggccaacatc ctgaacaagc cctacgaggt catcttctcc
      361 gagttcgagg gctcgatcga tcccacgacc atgcagggtt cgggggacgt caagtaccac
      421 ctgggcgccc gcggggagca ccagtccccg agcggggcga ccgtcaagct cacgctcgcc
      481 tcaaacccga gccacctgga ggccgtcgac cccgtggtcg agggcatggt ccgggcgcgg
      541 caggagaccc tgcgcgaggc ctcggcctcg gcgccgggcg gagactacct ggatgccgtc
      601 gtcccgatcc tcatccacgg ggacgcggcc ttcgccggcc agggcgtggt ggccgagacg
      661 ctccacatga gccagctccc gggctaccgc acgggcggga ccatccacat cgtcgtcaac
      721 aaccagatcg gcttcaccac gggcccggcc gaggcgcgga gctcggagta tgcttccgac
      781 atcgcccgga tgatccaggc gcccatcttc cacgtgaacg gggacgaccc cgaggcctgc
      841 acccgggtcg cccggctcgc cctcgatttc cggcaggtct tcaacaagga tgtcgtcatc
      901 gacatgctgt gctaccgcgt ccgcgggcac aacgagggcg acgagcccac ctatacccag
      961 cccgtcctct accggaagat cgaggaaaag cgctcggtcc ggaagctcta caccgagctc
     1021 ctgctccgcc ggggcgacat ggagcccgac gaggcggagc gcatgctgga ggattaccgg
     1081 cagcgcctgc aggacgcctt cgagcgcacg aaggaactcg ccgaacggcc cgagccgccg
     1141 gccgaacacc tgctagtcaa gccccggtcg gagcggctgc ccgacgtcgt cacctcagtc
     1201 gacgtggcgg ccgtggaccg cgtgatccag gccctgcaga ccttccctga atggttcagc
     1261 ctgcacccga agcttgcccg ccagttcgag cgccgacacc ggctgttcca cgaggaaaag
     1321 cggatcgact ggggattcgc cgaggcgctc gccttcggga ccacgctcga cgagggcatc
     1381 cgtgtccgcg tctccgggca ggactcgcgc cgcggcacct tcagccatcg ccacgcggcg
     1441 gtgtacgacc aggagtcggg cgccgagtac atcccgctga accacatccg cgacgggcag
     1501 gcacacctct acgtctacga cagcctgctg tcggagtacg cggccgtcgg cttcgagtac
     1561 ggctactcgg tctcggtcga aaacgccctc gtcatctggg aggcgcagtt cggggacttc
     1621 gcgaacggcg cgcagatcgt gttcgaccag ttcctggcgg cggccgagga gaaatggggc
     1681 cagaagagcg gccttgtcct gctcctcccg cacggctacg aggggcaggg gcccgagcat
     1741 tcctccgcgc gcctcgagcg cttcctgcag ctgtgcgccg agggcaacat gatcgtggcg
     1801 aacctgacga cgccggccaa catgttccac ggacttcggc gacaggtgca ctcgaccgtc
     1861 cggaagccgc tggtcatcat gtcccccaag agcctgctcc ggcatcccct tgccgtctcg
     1921 gtgccggagg acttcacgga gggcggtttc aggactctga tcccggccga cgtgccggac
     1981 ccggccgccg tggaccgggt ggtcttctgc tcgggcaagg tgtactacga cacgctgcag
     2041 gccctgcaga aggacgaggc ccgggccgga agggtcgcgc tcgtccgcgt ggagcagctg
     2101 tacccgttcc cgaagcacga gatcgcggac gagctcgacc gcttcgccgg cgtggaggac
     2161 gtcgtctggc tgcaggagga gccggcgaac atgggcgcgt ggagcttcgt ccggtggcgg
     2221 ttcgacgaga tcctcgagga gcgcgtgggc gaccgcaccc accgcatccg ctacgtgggt
     2281 cgtcccgcct cggccagccc cgcgaccggc agcacccacg tgcacgagcg cgagcaggcg
     2341 gcactcatcg cccgggcgat cggagcggcg tcgcagtgaa aggccgcggc gcccggcgcc
     2401 gatccacgaa aaaagggtga ctccctgccg ggaatcaccc tcttcgaaag tcacatcgcg
     2461 gcccgtgtga ccgcgcgccg atcctagtag taacgacccc gcttccgggc gttgcgggcc
     2521 gccttctcgc gggccacgcg gcgctcttcg gacggcttcg tgtaggccat gttggcgcgg
     2581 aagatgcgca ggacgcggct gcggttcact gcgcgcttga aacggcgaag ggcacggtca
     2641 atcgactcgt tatcgcggac tttgatgcct acggacacgg ggtatcggta aatcgctgat
     2701 ggacgtagaa aagaaagata cggcggcgtt tcggctcgcg caactcggga atcggcgtgc
     2761 cttgtggccc gcggcaccgc ggagtaagtt tccggccccg ccagacgctt tcaccatgcg
     2821 actcctcgtg gccacgcgca atccgggcaa gctcgccgag ttccgccagc tgctcggcga
     2881 ccttccggtc gtcgtcgtcc agatcgacga ggtggacggg gcgcccgacg tggtcgagga
     2941 ccggcccacc ctggagggca acgcccggaa gaaggcgcag acgctcgccg acttctcggg
     3001 cctgcccacg ctggcggacg acacggggct ggaggtcgat gcgctcggag gccgtcccgg
     3061 ggtgttttcg gcccggtatg ccggcccgga ggaggatccg gcggccaacc gccgccttct
     3121 cctggccgag ctcgcggggc gtccggaccg gtccgcccgg tttcgcgccg tcatcgcttt
     3181 tgcggagggc ggatcgtgtc ggctcttcga gggtatctgt gagggccgca tcgcggagac
     3241 gccgcggggc gaaggcgggt tcggatacga tcccctgttc gtgcccggcg gcgaggatcg
     3301 cacgttcgct gagctggacc cccaggccaa gaacgcgatc agtcaccgcg gacgggctct
     3361 ccgcctgttt tccgaatacc ttcacgagcg actcgcccgg tagcatggtg gcactcgtcc
     3421 agcgcgtgag cgaagcctcg gtcgcgatcg acggtcgcga ggtcgcctcc gtcgggcgcg
     3481 gcctgctcgt gctcctcggc gtgcaccgca cggacaccgg ggccgaggcg agctggctgg
     3541 cccgcaaggt cgccaacctg cgcatcttcc ccgacgcgga cgggctcatg aacctttcgg
     3601 gatcggatac gggggccgag gcgctcgtcg tctcccagtt cacgctctac ggcaacacgg
     3661 acaagggcaa ccggccgtcc ttcgtggact ccgccgaacc cgccctggcc gagcccctgt
     3721 accgcgcgtt cgcccgggac ctcggggagc atctcgggcg gaccgtgccc accggcgagt
     3781 tcggcgccgt catgcaggtc gccctcgtga acgacggacc cgtgacgctg tgggtggagc
     3841 ggcgcgcgtc gtgacggccc cgggccccga tatcacccgg aacggccacg gcatgctgac
     3901 cgactcgttc ggccgcttcc acgactacct gcgcttctcg ctgacggagc ggtgcaacct
     3961 ccggtgcacg tactgcatgc ccccggaggg cgtcgagctc aagccgcgct ccgacatcct
     4021 caccttcgag gagatcgagc gcctttcccg cctcttcgtc gcggaaggcg tccgcaagat
     4081 caggctgacg ggcggcgagc ccctggtccg ccggcacgtc gaggagctgg tggaccgcct
     4141 gggcgcgatc gagggcctgg aatccctcgc cgtgaccacg aacggcctgc tcctcgagcc
     4201 gctgctcccg cgcctgcagg cgggcggcat caccgagatc aacgtgagcc tggacacgct
     4261 ccgggaggac cgcttccagc agatcacgcg ccggcccggg ctcgaacgcg tgcgctccgc
     4321 gatcgatgcg gccatcgggg cgggctaccg tccggtgaag gtcaactgcg tgctgatgcg
     4381 cggcgtgaac gacgacgaga tcggcgattt cgtgcgctgg acactcgacc gccccgtctc
     4441 ggtgcgcttc atcgagtaca tgccgttcga gggcaactcg tgggacgaca cccgcatggt
     4501 gccggacgcc gaggtcctcg atcgcatcct ggccgagcat ccggggctcg ttcggctcga
     4561 cgactccccc acgtccacgt cccgcacgta ccgcgtcccc ggggccgtcg ggacggtcgg
     4621 actggtgtcc tcgatgacgg ccgccttctg cccggggtgc aaccggctgc ggatcacggc
     4681 cgacggccgc ctcatggtct gcctgttcgg ccgcgcgggc gtcagcctgc gggatgccat
     4741 gcgcagcggc gacgacgacg acgcccttcg ccgcgtggtc cgctccgcgg tgctgggcaa
     4801 gaaggcggcg cacgccggga tggaggtgct ggccgcgcag accgatcgtc cgatgatcct
     4861 catcggcgga tgagccgcat gcgggcaggc cccgaatcgc cttgccctcc gtcgcgcgcc
     4921 cggcctatct tggcgtccgt ccgtccatcc gtttccgcgc ttccatgaca gagtccccgt
     4981 ccctgacgca catcgatccg cacggcggcg tgcacatggt cgacatctcg gacaaggacg
     5041 acacggtccg cacggccgta gccgccggcc gcatcgtcgt gggagacgag gccttcggga
     5101 tgatccgcag ccggaacgtg tcgaagggcg acgtgctgct gacggctcag atcgccggca
     5161 tcctgggagc caagcagacc agccggctca tcccgctctg ccacgaagtg tccctgcggg
     5221 gcgtggacgt cgagctcacg ctcgcggacg atgcgcctgc catcgacatc cgggccttcg
     5281 ccaagagtgt cggccccacg ggggtcgaga tggaggcgct cacggccgtc tccgtcgcgg
     5341 cgctcaccat ctacgacatg tgcaagtcgg tctccaaggc gat
//
LOCUS       DBNHQZ010000391         5472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1742631, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000391 DBNHQZ010000000
VERSION     DBNHQZ010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1742631"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="AC16YU_10635"
     CDS             <1..984
                     /locus_tag="AC16YU_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6065724.1"
                     /translation="AFVLRTPDDAARLKDALGRIAPAHAVIVGAGFIGLELATLLRER
                     GWRVTVLDANGPLGRHLDPPMVERLDEVLARFGVAVRRERAASLRRPGMGPAVVLKTD
                     RGELVGARIVILAAGIVPLTGLGGDAIRRTDGGTYLVDASMRTSARGVWACGDCVSVP
                     ALPDGSHRWIPLSPVAFRGARVAGHNAARQGRTAELTMPRPAGSYTARIADVEVASVG
                     LSARDARTRGLDADYAEIRSVTASSLHPHSRPVHVGLVFERPQGRLLGAQVIGGYGAA
                     LRADVVAAVLRKGGSVTDLYEVDYAYAPPVAPAFDPLMVAARSAQKALGRR"
     gene            1010..2482
                     /gene="rsmB"
                     /locus_tag="AC16YU_10640"
     CDS             1010..2482
                     /gene="rsmB"
                     /locus_tag="AC16YU_10640"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR6065725.1"
                     /translation="MPDPPRRGSRPPGHGGGRPQRGGQGRGRSAGRPGDRREPRPRSD
                     ESRVEAVRELLRVEEDRAFVGLMRGAGPDRDEDTDRRVTDYVAGVTRWKRWLDHLIAG
                     VYRGDLEAMEAPLRQMLRIGAYDLVIRRIAPHAAINEAVDAARRAVRPEAAGVTNAVL
                     RAVSQLDPAAVAVDTEDPAERLAVRWSHPTWLVRRWLDRYGEKDTVRLLERDNRAPVH
                     GLRIDGPPAARASFRDMLKEARIDWEPSPYLDDFVRVIRLQPVIRARWIERGYCTVQD
                     ESTGLVVRVLDPQPGEHVVDACAAPGGKSLYASSLMGGRGRITAVDQNAARLDLLSRS
                     ASKAPTPIESVVADFADWAAGHAGDPADRVLLDAPCSGLGVMARRADLRWRRGPESFA
                     ELTALQDRLLDAASLVVRPGGLLVYATCTIEPEENAERVHAFLGRHPDFSLEPAGGAV
                     PARLLDADGSLLTLPFRDDVDGAYAARLRRAGQPKPTSGG"
     gene            2574..3668
                     /locus_tag="AC16YU_10645"
     CDS             2574..3668
                     /locus_tag="AC16YU_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6065726.1"
                     /translation="MTFLRSAVLACLGLGLAAGQADAQRVAKYGADFLAGGVGARALA
                     MGGAHVGMASDVHATYWNPAGLQAMGSAEIAYMHAERFAGVVSFDYGAVALPLSARST
                     VSLAFFRSGVNDIKNTLDAWDPERDQPRPNPDQYVTTFSAADHALFIGYSRALAGGLD
                     LGVTAKIIRRKIGSFAGAWGYSFDVGVRYVRGSLALGAVVQDATTMLQSWSVDADRLA
                     PIRDVFGDELPSGGTELVLPVLRLGSAYSWAVPGGRLALAADLDLSFDGQSANVLDVG
                     GGAVAFHPRAGFEYTFRDVVALRGGIARVLDTEAEGLDLSPAVGSGLRLGRFAIDYGF
                     GDFSGLVSELGYSHRVSVSFDLARARPAAE"
     gene            3736..4701
                     /locus_tag="AC16YU_10650"
     CDS             3736..4701
                     /locus_tag="AC16YU_10650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6065727.1"
                     /translation="MTSDARRETVALVVPLYNEEDVLPALVREVEAFRAAHPEIDQVV
                     LVDDGSTDRTAQLAKMLTEGRSGYVLLRFSRNFGHQLAVTAGLHFVRSDAAVVLDGDL
                     QHPLATVGHMIERWRDGYDVVYGIRRSHRADKWPERLAARVYYRLFRKMTGVDAPVDA
                     ADFRLMSRAVLDAYRQFEEQQPYVRGLVAWLGFNQVGVEYEAPARAAGRSKYAWRDRF
                     RLGIDGLASFSARPLRYAVRLGVAVSGLAFLGLVWVLISKLVLGNTVPGWASVTFVGF
                     FFGGVQLFFLGVLGSYVARVYDEVKGRPRYVVREHWTSPGPRGDD"
     gene            4778..>5472
                     /locus_tag="AC16YU_10655"
     CDS             4778..>5472
                     /locus_tag="AC16YU_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6065728.1"
                     /translation="MKRDDWIGLSVSVLMHAVVLLGLSFLTMGAVDPTPIGFVEVEFG
                     PFAEGRPVSRAPQSQAEQTPQPADETEPTEPQPAPAPREARPVDLPDQEVADAETVQE
                     ATSEVVAPEQRPESESELETEQPSDSRPIRPLGSGDPEGDDRTQEGREGAGADETRSA
                     PYQIEGLNRIPVATVTPRYASEVNADITVQITVDPQGRVIGSLPLRKADARLEQAVSD
                     ALARWRFNPLPPGA"
BASE COUNT          706 a         1931 c         2015 g          820 t
ORIGIN      
        1 gccttcgtcc tgcgcacgcc ggacgacgcc gcgcgtctga aggacgcgct cggccggatc
       61 gcgcccgccc atgccgtgat cgtcggggcc ggcttcattg gcctggagct cgccacgctg
      121 ctccgggagc gcggctggag ggtgaccgtg ctcgacgcca acggcccgct gggccggcac
      181 ctcgatcccc cgatggtcga gcggctggac gaggtcctgg cccgattcgg cgtcgccgtg
      241 cggcgcgagc gggcggcctc cctgcggcgt cccggcatgg gccccgccgt cgtcctgaag
      301 acggatcggg gcgagctcgt cggggcgcgc atcgtgattc tcgccgcggg catcgtgccg
      361 ctcacgggtc tcggcggcga cgcgatccgc cgcactgacg gcggaaccta cctcgtggat
      421 gcatccatgc gcacgagcgc ccggggcgtg tgggcgtgcg gcgactgcgt ctccgtgccg
      481 gcgcttccgg acggaagcca tcgctggatt cccctttctc ccgtcgcgtt ccggggcgcc
      541 cgggtggcgg ggcacaatgc cgcgcggcag gggaggaccg cggagctgac catgccccgg
      601 cccgcaggct cgtacacggc gcgcatcgcg gacgtcgagg tggcctcggt cggcctgtcc
      661 gcccgggatg cgcggacgcg gggcctggac gcggattacg ccgagatccg gagcgtcacg
      721 gcttcgtcgc tgcacccgca ctcacggccg gtgcatgtcg gcctcgtatt cgagcggccc
      781 caggggcgcc ttctcggtgc gcaggtcatc gggggctacg gggcggcgct ccgggcggac
      841 gtggtggccg ccgtcctgcg gaagggcggc tccgtgacgg acctgtacga agtcgactat
      901 gcctacgcac ccccggtggc accggccttc gatcccctca tggtggcggc gaggtcggca
      961 caaaaggcgc tcgggcgacg ttagctcccg actccctgat cccgattcca tgcccgatcc
     1021 tccccgtcgc ggaagcagac cacccggaca cggcggaggc cgtccccagc ggggcggcca
     1081 gggcagggga cgaagcgcgg gacggcccgg ggaccgccgg gaaccacgcc cgcggagcga
     1141 cgagtctcgt gtcgaggcgg tccgagagct gctccgggtg gaggaggacc gggcgttcgt
     1201 cgggctgatg cgtggagcgg ggccggaccg cgacgaggac acggaccgcc gcgtcaccga
     1261 ctacgtggca ggcgtgacgc ggtggaagag gtggctcgat cacctcattg ccggcgtcta
     1321 ccggggcgat ctggaggcga tggaggcgcc gctccggcag atgctccgca tcggcgccta
     1381 cgacctggtg atccgccgca tcgcacccca cgcggccatc aacgaggccg tggacgcggc
     1441 acgccgggcg gtcaggcccg aggccgccgg cgtgaccaac gccgttctgc gggccgtctc
     1501 gcagctcgat cccgccgccg tcgccgtcga cacggaggac ccggcggagc ggctggccgt
     1561 gcgctggtcg cacccgacgt ggctcgtgcg ccggtggctg gaccggtacg gggaaaagga
     1621 caccgtgcgc ctgctcgagc gcgacaacag ggctccggtg cacggccttc gcatcgacgg
     1681 ccctcccgca gcaagggcgt cgttccggga catgcttaag gaagcacgaa tcgactggga
     1741 gccatcgcct tacctggatg acttcgtgcg cgtcatcagg cttcaacccg tcatccgggc
     1801 ccggtggatc gagcgggggt actgcacggt ccaggacgag agcaccggcc tcgtggtccg
     1861 cgtgctggac ccgcagccgg gagagcacgt ggtggacgcg tgcgcggcgc ccgggggcaa
     1921 gtccctgtat gcgtcgtcgc tcatgggcgg gcgcgggcgg atcacggccg tcgaccagaa
     1981 cgcggcgcgg ctggacctcc tgtcgcgatc cgcgtcgaag gcgccgaccc cgatcgaatc
     2041 cgtggtggcc gacttcgcgg actgggccgc gggccatgcg ggcgatccgg cggaccgggt
     2101 gctgctcgac gcgccgtgct ctggcctggg agtgatggcc cgccgcgcgg acctgcgctg
     2161 gcgacgcggc cccgaatcgt tcgcggagct caccgcgctg caggatcgac tgctcgacgc
     2221 cgcctcgctg gtcgtccggc cgggaggcct gctggtgtac gccacctgca cgatcgagcc
     2281 cgaagagaac gccgagcgcg tccacgcctt tctcgggcga caccccgatt tcagcctgga
     2341 gccggccggc ggcgcggtcc ctgcccggct gctcgacgcc gacggctctc tcctgacgct
     2401 gcccttccgc gacgacgtgg acggcgcgta cgccgcgaga ctccgtcgcg ccgggcagcc
     2461 gaagcccact tccggcgggt aacggccggg tcaatgcccg gccccgagaa acccgcgctc
     2521 ctctgccgca ttccggagtg cgtgagtcgc acccgcccga acccttcgtc gcgatgacct
     2581 ttctccgttc cgccgtcctc gcctgtctgg gcctcgggct cgccgccggg caggccgacg
     2641 cgcagcgcgt ggccaagtac ggggcggact tcctcgccgg cggcgtggga gcgcgtgccc
     2701 tggccatggg cggcgcccac gtcgggatgg cctcggatgt gcacgcgacc tactggaatc
     2761 cggcgggcct gcaggcgatg ggctcggccg agatcgccta catgcacgcg gagcgtttcg
     2821 ccggcgtcgt ctcgttcgac tacggagcgg tcgccctgcc gctttcggcg cgctccacgg
     2881 tgtcgctcgc ctttttccgc agcggggtca acgacatcaa gaacacgctg gacgcctggg
     2941 atcccgagcg ggaccagccg cgcccgaacc ccgaccagta cgtcacgacg ttttcggccg
     3001 cggaccacgc gctgttcatc ggctactcgc gcgcgctcgc gggcggcctg gatctcggcg
     3061 tcacggcaaa gatcatccgc cgcaagatcg gctcgttcgc gggcgcctgg gggtactcgt
     3121 tcgacgtggg cgtccgatac gtccggggat cgctcgcgct cggcgccgtg gtgcaggacg
     3181 cgaccacgat gctgcagagc tggagcgtgg acgccgaccg cctggccccc atccgcgacg
     3241 tgttcggcga cgagctgcct tccgggggga cggagctggt ccttccggtg ctccgtctcg
     3301 ggagcgcgta ctcgtgggcg gtccccgggg gtcgcctggc gctggcggcg gacctggatc
     3361 tctcgttcga cgggcagtcc gcgaacgtgc tcgacgtcgg cggcggcgcg gtggccttcc
     3421 atccccgggc ggggttcgaa tacacgttcc gggacgtggt ggcgctccgc ggcgggatcg
     3481 cgcgcgtcct ggataccgag gcggagggcc tggacctgag cccggcggtc ggctccggac
     3541 tccgcctggg ccggttcgcg atcgactacg ggttcgggga cttttcgggc ctggtctcgg
     3601 agctcgggta ctcgcaccgc gtgtcggtct cgttcgatct cgcccgcgcg cgccccgcgg
     3661 ccgagtagcg gagccgggcg tgcttgcgcc gggtcgggta ccggtgtatc tacggctgtt
     3721 tcttcgatcc gaacgatgac gagcgacgcc cgacgcgaga cggtagccct ggtcgtgccc
     3781 ctgtacaacg aggaggatgt cctgccggcc ctggtccgcg aggtcgaggc cttccgcgcc
     3841 gcgcacccgg agatcgacca ggtcgtgctg gtcgatgacg ggtccaccga ccgcacggcg
     3901 cagctcgcga agatgctgac cgaggggcga agcgggtacg tccttctccg gttctcgcgg
     3961 aatttcggtc accagctcgc cgtaacggcc ggactacatt tcgtgcggag cgatgcggcc
     4021 gtggtgctcg acggggacct tcagcacccg ctcgcgaccg tcgggcacat gatcgaaagg
     4081 tggcgcgacg gctacgacgt cgtgtacggg atcaggcgct cgcaccgggc cgacaagtgg
     4141 ccggagcgac tcgcggcgcg ggtctattac cgcctgttcc ggaagatgac cggcgtcgac
     4201 gcgcccgtcg acgcggcgga cttccggctc atgtccaggg ccgtactcga cgcgtaccgg
     4261 cagttcgagg agcagcagcc gtacgtgcgg ggcctggtgg cctggctggg gttcaaccag
     4321 gtcggcgtcg agtacgaggc gccggcgcgg gccgcgggcc gcagcaagta cgcctggcgg
     4381 gatcgtttcc ggctcggcat cgacggcctg gcctccttct cggcgcgtcc gctccggtac
     4441 gccgtgcgcc tgggcgtggc cgtctccggg ctcgcattcc tggggctcgt ttgggtcctg
     4501 atctccaagc tcgtgctggg caacacggtg ccgggctggg cgtcggtgac gttcgtcggg
     4561 ttcttcttcg ggggggtgca gctcttcttc ctgggcgtcc tgggctcgta cgtggcccgg
     4621 gtctacgacg aggtcaaggg ccgtccgcgc tacgtggtcc gcgagcactg gacgtctccg
     4681 ggtccgcgcg gcgatgactg atcccaagga caccgcgcgt ggaatcccgc ccgagcctgc
     4741 cgcgtacgag accggaatcc gcaactccga gcctgccatg aagcgagacg actggatagg
     4801 cctgtccgtg agcgtgctca tgcacgccgt tgtcctgctc ggtctttcct tccttacgat
     4861 gggagccgtc gaccccacgc cgatcgggtt cgtggaggtg gagttcggcc cgttcgcgga
     4921 aggccggccc gtgtcgcgcg ccccgcagtc ccaggccgag cagaccccgc agccggccga
     4981 cgagacggag ccgacggagc cgcagccggc cccggctccc cgcgaggctc gcccggtgga
     5041 cctgcccgac caggaggtcg ccgacgccga gaccgtgcag gaggccacgt ccgaggtggt
     5101 cgccccggag cagcgccccg agtcggagtc cgagctcgag accgagcagc cgtcggacag
     5161 ccggcccatc cgtccgctgg gcagtggcga tcccgagggt gacgaccgga cccaggaagg
     5221 ccgggaggga gccggcgccg atgaaacgcg gagcgcgccc taccagatcg aggggctgaa
     5281 ccggattccg gtggcgacgg tcaccccccg gtacgcgtcg gaggtgaatg ccgacatcac
     5341 cgtgcagatc acggtcgacc cccagggacg cgtgatcggc tcgcttccgc tccgcaaggc
     5401 cgacgcgcgc ctcgagcagg ccgtgagcga cgccctggcc cgctggcgct tcaacccgct
     5461 tccgcccggc gc
//
LOCUS       DBNHQZ010000392         3175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2960760, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000392 DBNHQZ010000000
VERSION     DBNHQZ010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2960760"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..555
                     /locus_tag="AC16YU_10660"
     CDS             13..555
                     /locus_tag="AC16YU_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR6065729.1"
                     /translation="MNRRTGLLIAMLAGILAAVQPASAQGLRVGYADPEVIISYMPEY
                     QQVQDQLGQEYRTSQEALQALAQDFQERVERYQKQQPLLTEERRAERERELAQLQTEL
                     QESAARKDQELAARQEELMAPLLDRVQSVIDDIAVAKGLDIVMRSPTLLYVNEDRVVN
                     INLDIAKALGIEIDEEAAQN"
     gene            671..1507
                     /gene="panB"
                     /locus_tag="AC16YU_10665"
     CDS             671..1507
                     /gene="panB"
                     /locus_tag="AC16YU_10665"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR6065730.1"
                     /translation="MSTRIRTVSDVSDVKRITTQTLQEMKAAGIPVAMLTAYDFTSAR
                     ILDGAGIDVLLVGDSASNVMAGHETTLPITLDQMIYHAQCVVRGVQRAFVVVDLPFGS
                     FQGNSRKALASAIRVMKESGAHGVKLEGGQAVVPTVERLLEAGIPVMGHLGLTPQSIY
                     RFGTYKVRAQEEDEADRLREDAVLLERAGCFAIVLEKIPAALAGEVSRSLSIPTIGIG
                     AGAGCDGQVLVTHDALGLSTDFNPRFVRRYANLDRTMAEAVQRYIQDVRNPAFPTDEE
                     SY"
     gene            1510..2301
                     /gene="surE"
                     /locus_tag="AC16YU_10670"
     CDS             1510..2301
                     /gene="surE"
                     /locus_tag="AC16YU_10670"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6065731.1"
                     /translation="MPEGEKPLILVCNDDGIDAPGISALAAALDGLGEVRVAAPVHEQ
                     SAVGHAITVRDPVRAFPWPFRGPSGSIPAHAVTGTPADCVKLAIDKLLPRAPDLVVSG
                     INQGPNTAVNVIYSGTVSAATEAAILGIDAMAVSLCRWGGGDFEAAGLFARRIAEAVL
                     RKRLPPGVLLNVNVPDLPYAQIGGIRITRQARARWEESFHERRDPLDRPYYWLTGQFV
                     NLDEGEDTDIGAIDAGFVSVTPVHHDMTAHAHIRTLREWEWTDNR"
     gene            2325..2792
                     /locus_tag="AC16YU_10675"
     CDS             2325..2792
                     /locus_tag="AC16YU_10675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6065732.1"
                     /translation="MRRIQVELDRVDDAFHFVARNAAGFEVHIDDATARDDGVAHGVG
                     PMQLLLMGLAGCSGVDVASILRKSRQSIDALRIQVTGLRAENETPSPYLGFHMHFELE
                     GDVDPKRVERAVVLSVTKYCSAAATLRHAAPVTASYSVNGEHHDTDVRLGPDS"
     gene            complement(2819..>3175)
                     /locus_tag="AC16YU_10680"
     CDS             complement(2819..>3175)
                     /locus_tag="AC16YU_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065733.1"
                     /translation="MHAWWRGLIRLRASAFGEVLRIGTAVPGDYVRFTAPDDPAGLAY
                     VVGGRLLVAVNVGPKGMTVEGVDLPGGTWRMVSDGRRVDHEATFDEPDATLEGGRTRV
                     VLPPRTMKIWVLDPGS"
BASE COUNT          543 a         1075 c         1102 g          455 t
ORIGIN      
        1 ccacttacga tcatgaacag acggacaggc ttgctgatcg cgatgctcgc cggaatcctt
       61 gccgcggtgc agcctgcatc cgcccagggg ctccgcgtcg ggtacgccga tcccgaagtc
      121 atcatcagct acatgcccga gtaccagcag gtgcaggatc agctcgggca ggagtacagg
      181 accagccagg aggccctgca ggcgctcgcc caggatttcc aggagcgcgt ggagcggtac
      241 cagaagcagc agccgctgct gacggaagag cggcgggcgg agcgtgagcg tgaactcgcc
      301 cagctccaga ccgaactcca ggagtcggcg gcgaggaagg accaggagct cgcggcgcgc
      361 caggaggaat tgatggcccc gctcctggac cgggtccaga gcgtcatcga cgatatcgcc
      421 gtggccaagg gcctggacat cgtcatgcgc tcgcccacgc tcctgtacgt gaacgaggac
      481 cgcgtcgtca acatcaacct cgacatcgcc aaggcgctcg ggatcgagat cgacgaagag
      541 gccgcgcaga actgatcatc gcggcggaag ccgcgcgggg ggcgggcatc gagaccggtg
      601 cccgcccttc ttgttacggg ccgtgccgcc ccgagcggta tattcgcgcc gacccgaccc
      661 gatcgccgac atgagcacgc gaatacgaac ggtgtccgac gtttcggacg tcaagcgcat
      721 caccacgcag acgctccagg agatgaaggc cgccgggatc cccgtggcca tgctcacggc
      781 gtacgacttc acgtcggccc gcatcctgga cggcgcggga atcgacgtgc tcctggtcgg
      841 ggactcggcg tcgaacgtga tggccgggca cgagaccacg ctcccgatca cgctcgacca
      901 gatgatctac cacgcccagt gcgtggtccg gggcgttcag cgggcgttcg tggtggtgga
      961 cctcccgttc ggatccttcc agggcaactc ccggaaggcg ctggcctcgg ccattcgagt
     1021 catgaaggaa tccggggcgc acggcgtcaa gctggagggc gggcaggcgg tcgtcccgac
     1081 ggtcgagcgg cttctcgagg ccgggatccc ggtcatgggg cacctcggac tcacgccgca
     1141 gagcatctac cgcttcggaa cctacaaggt cagggcgcag gaggaggacg aggccgaccg
     1201 gctgcgcgag gacgccgtcc tgctggagcg ggccggctgc ttcgcgatcg tgctcgagaa
     1261 gatccccgcc gcgctggccg gcgaagtgtc caggagcctg tcgattccga cgatcggaat
     1321 cggcgcgggt gcggggtgcg acgggcaggt gctcgtcacc catgacgccc tcggcctgtc
     1381 cacggacttc aatccgcgct tcgtccgtcg ctacgcaaac ctggaccgga ccatggccga
     1441 ggccgtgcag cgatacatcc aggacgtgcg caaccctgcc tttcccaccg acgaggagag
     1501 ctactgaccg tgccggaagg ggaaaagccg ctcattctcg tctgcaacga tgacgggatc
     1561 gacgccccgg ggatctcggc cctcgcggcc gccctcgacg gcctgggcga ggtccgcgtc
     1621 gcggcgccgg tccacgagca gtcggcagtc ggccatgcga tcaccgtgcg cgatcccgtc
     1681 cgggcctttc cgtggccgtt ccgtggcccg tccgggtcca ttcccgccca cgcggtgacg
     1741 ggcactccgg cggactgcgt gaagctggcg atcgacaagc tgctgccgcg cgctccggac
     1801 ctggtcgtct cggggatcaa ccaggggccg aacacggcgg tcaacgtgat ctactccggc
     1861 accgtcagcg ccgcgaccga ggcggcgatc ctcgggatcg acgccatggc ggtgtcgctg
     1921 tgccggtggg gcggagggga cttcgaggcc gcggggctct tcgcccggag gatcgccgaa
     1981 gcggtgcttc gaaagcgtct tccacccggc gtcctgctca acgtgaacgt gccggacctg
     2041 ccgtacgccc agatcggggg catccgcatc acgcgccagg ccagggccag gtgggaggaa
     2101 tctttccacg agcgacggga cccgctggac cggccgtact actggctgac gggacagttc
     2161 gtgaacctcg acgagggcga ggacacggac atcggagcga tcgacgccgg attcgtctcg
     2221 gtcacacccg tgcaccacga catgactgcc catgcccata tccggaccct acgggagtgg
     2281 gagtggaccg ataaccgata atcgattcga ggaagctgaa aggcatgcgc cgtatccagg
     2341 tggagttgga cagagtggac gacgcgttcc atttcgtcgc gcggaacgcc gccggtttcg
     2401 aggtgcacat cgacgacgcc acggcgcgcg atgacggcgt ggcccacgga gtcgggccca
     2461 tgcagctcct gctcatggga ctcgccgggt gcagcggggt ggacgtcgct tcgatcctcc
     2521 gaaagagccg ccagtcgatc gacgccctgc gcatccaggt gacgggcctg cgggccgaaa
     2581 acgagacgcc atcgccctac ctgggcttcc acatgcactt cgagctcgag ggcgacgtgg
     2641 acccgaagcg cgtggagcgg gccgtcgtgc tgagcgtgac gaaatactgc tcggcggccg
     2701 cgacgctgcg ccacgcggcg cccgtcacgg cgagctactc ggtgaacggc gagcaccacg
     2761 acacggacgt ccggctcggt ccggacagct gaccggcccg ggaccctggc cgggcggatc
     2821 aggagcccgg atccaggacc cagatcttca tcgtgcgggg cggaaggacg acgcgggtcc
     2881 ggccgccctc gagcgtggcg tccggctcgt cgaacgtcgc ctcgtgatcc acccgccggc
     2941 cgtcggagac catgcgccag gtccccccgg ggagatcgac tccttcgacg gtcattccct
     3001 tgggacccac gttgacggcc acgagcaagc gccctccgac gacgtacgcg aggcccgccg
     3061 gatcgtccgg ggcggtgaag cggacgtagt cccccgggac ggccgtgccg atgcgcagga
     3121 cctcgccgaa ggcgctcgcc cggagccgga tgagccctcg ccaccaggcg tgcat
//
LOCUS       DBNHQZ010000393        13919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3369773, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000393 DBNHQZ010000000
VERSION     DBNHQZ010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3369773"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="AC16YU_10685"
     CDS             <1..1697
                     /locus_tag="AC16YU_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR6065734.1"
                     /translation="HDSSFPEHAIAYCLAEAGIGLADVDYVVFYDKPFLKFERLLETY
                     VATVPGGFPSFLKAMPLWLKQKLWIPDLIERELGYDGPVVFPEHHMSHAASAFYPSPF
                     ERAAVLTTDGVGEWATTSAGVGHGADLRILEEQHFPHSLGLLYSAFTYFCGFRVNSGE
                     YKLMGLAPYGEPAYVDRILEELVELRDDGSFRLNMEYFTYMAGLTMTGRRFARLFGGP
                     PRGAEAPITRREMDLACSIQVVTEEAVMRMARHVHEITGERDLCLAGGVALNCVANGR
                     LLREGPFERLWVQPAAGDAGGALGAALAAWHQHHGAPRTAASPDAMQGSYLGPAYGRD
                     ALLAFLEGLGIPFEEVGFEALPDHVAGLLAEGRVVGWFQGRMEFGPRALGARSILADP
                     RGRDVQRTVNLKIKFRESFRPFAPSVARDRLAGWFDLDADSPYMLLVAPVRDARIEGV
                     GLDRLKGIDSPIPAVTHVDGSARIQTVHRGTNPLYHRLLEAFEELTGCPVLVNTSFNV
                     RGEPIVCTPGDAWRCFRRTHLDVLVLGPFVVRRDRIPDADAEVIEAEDAARLYGLD"
     gene            1684..2274
                     /locus_tag="AC16YU_10690"
     CDS             1684..2274
                     /locus_tag="AC16YU_10690"
                     /inference="COORDINATES: protein motif:HMM:NF023096.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease family protein"
                     /protein_id="HIR6065735.1"
                     /translation="MDSTEPLATPSVPAEGWDDRTLTVGEVWRYLARILPLVVVVTIG
                     VHALAWGAPGYGDDLLVGGPWIGILVYLLSVVVHEALHVAAMVAFGVPVRTIRVGSRL
                     REGVVYVHAGRDLTVGPYRVVLLLPALVLGVVPTVAGLVAGSWAWTLYGFVMLISAAG
                     DFAVIDRLRGLPPGTVVRDHPEKVGCQVRLPEPALP"
     gene            2348..3661
                     /locus_tag="AC16YU_10695"
     CDS             2348..3661
                     /locus_tag="AC16YU_10695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6065736.1"
                     /translation="MATRREFFGTIGRPAAAALATATFHPAAASRLFDVLAEYRGTPS
                     EAASDEELWREVQQAFTVDRTLVNLNNGGVSPAPAVVQEAMKRHLDYSNEAPVYTMWR
                     ILEPQREGVRQRLARAFGCDPEEIAVVRNASEGLQTCQLGIDFEPGDEVLTTTHDYPR
                     MITTFQQRERRDGIRLVQFPLPIPAEDPAEVVRLFESHITPRTKAILMCHIVNITGQI
                     LPVKAVVQMARARGIPVIVDGAHSFAHFHFTQADLDCDYFSTSLHKWLFAPHGTGFLY
                     VRKERIPDLWPMMAAPETLDGDIRKFEEIGTHPAANFLAIGEALTFHQGIGPERKEAR
                     LRYLRDYWATRLLEHDRVRLHTSLKPEFSCTIANVQIEGVASVALGNYLWDRHRIIVT
                     PIVHPDFEGLRITPNVYTTLEELDRFVDAMEHVVRNGLPEPYGTP"
     gene            3678..4877
                     /gene="lhgO"
                     /locus_tag="AC16YU_10700"
     CDS             3678..4877
                     /gene="lhgO"
                     /locus_tag="AC16YU_10700"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HIR6065737.1"
                     /translation="MTDPVVIIGGGIVGLSTAWHLSRHPEAGPVLVLEKEDEVGLHQT
                     GRNSGVIHSGIYYRPGTLKARLCRDGRQRLAEFCEERGIPYEFCGKVIVAVDDAEAGR
                     LPGLLERGRANGVSCRAIDRAELREREPHVEGRAAILVDDTGIVDFLEVTRHLAALLE
                     SSGHEVRRRSGVRAIGQEGGGYRLDTTAGPVRARFLVNCAGLHADRVARMAGVDPGVR
                     LVPFRGQYYELPGEGRRFCRHLVYPVPDPAYPFLGVHLTRRIDGRVECGPNAVPAMAR
                     EGYRFRDVNLGDLREIVAFLGFRRLALRHWRMGSAEIARAVSTRRFVASLRRLVPGIE
                     RGDLSAAPSGVRAQALTPGGELADDFVIRDARGAVHVVNAPSPAATASLAIGAFLAQH
                     VLGRIAA"
     gene            5333..5806
                     /locus_tag="AC16YU_10705"
     CDS             5333..5806
                     /locus_tag="AC16YU_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065738.1"
                     /translation="MSRPASVHPSETLYSSCRYVDDVRLYMGRAVLTPDEIRVHGWAG
                     THHVSMQIPLKDVTGLARSAQGRGPMLVLDTADGRTVALGISAPGLWRWAVRQRLPAG
                     HPLAPSADEPVPDAPAPRRESHAFLLRVAYLAGDRVPATADGDIRWRPLGELTSA"
     gene            complement(5814..6422)
                     /locus_tag="AC16YU_10710"
     CDS             complement(5814..6422)
                     /locus_tag="AC16YU_10710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6065739.1"
                     /translation="MSALPAAAGNLPDPVPVVTVRVAGPDDFPYAEQICAEMADAARI
                     RGTGIAKRTPEYIRGKMEEGKAIIALSDDRLAGFCYIETWEHGRYVANSGLIVVPEFR
                     KLGLARRIKRRAFRLSRERYPDAKLFGITTSLAVMKINADLGYRPVTFSELTQDDVFW
                     RGCQSCPNVDILTRTRKQHCLCTGMLFDPGAPPPRPEDHPDE"
     gene            6580..7773
                     /gene="aroC"
                     /locus_tag="AC16YU_10715"
     CDS             6580..7773
                     /gene="aroC"
                     /locus_tag="AC16YU_10715"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR6065740.1"
                     /translation="MIRYLTAGESHGEALVGIVEGMPAGVPVTAADLDEHLARRWLGF
                     GRGGRARIEKDHVHIYSGIRFGRTMGSPIALRIDNAAYLRDRSGWPDIMAIEETGDPV
                     EKVTLPRPGHADLAGVQKYGFDDIRPVIDRSSARETAMRVACCSVARRLLLEFGIEVG
                     SHVLRIGEVAADGEATGATVRRLLEEGGASALYAAADRDEVRMLDADASARAIDRIVK
                     AKEDGDSLGGVYEVVVTGVPPGLGSYVHWDRRLDGQLAQAILSIQAQKAVEVGDGVAA
                     ASLQGSRVHDPIRRDGGRFTRESNRAGGVEGGVTTGMPVVVRGFMKPIPTLIRPLGTV
                     DIATGEAQPTRYERSDVTSVPAASTVAEATVALVIANAFLEKFGGDSLDEIRGRFERE
                     VRGSG"
     gene            complement(7802..8437)
                     /locus_tag="AC16YU_10720"
     CDS             complement(7802..8437)
                     /locus_tag="AC16YU_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065741.1"
                     /translation="MKGRIILRRAAPLAAALLLAAAPASGQDRTDDRAAMREARQATR
                     IERQEARRDAALTRLSERLELTDAQKAAVGEVMQADEETGRAGDGWTVAAVLARDLTP
                     AQVDKLFEASREAGIYRRGMADGFRRGAGQAGRRVHRVAERPARVPEAMARLRDARIE
                     GMRKALDLSDEQVRVLRIHDALAGVMGAGPAAVDGRTGVRRGFRGDMRFGR"
     gene            complement(8651..9607)
                     /locus_tag="AC16YU_10725"
     CDS             complement(8651..9607)
                     /locus_tag="AC16YU_10725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR6065742.1"
                     /translation="MRLSDPDSRIFVAGHRGLVGSAVVRALGAAGYGNLVTRARGDLD
                     LRDQAAVARFFDEERPEFVVLAAARVGGILANATWPADFLYDNLAIQTSVIRAAHLAG
                     VRRLVFLGSTCIYPRLAPQPLREESLLTGPLEPTNEWYAVAKIAGLKLCQALRRQHGA
                     DFVSLMPTNLYGPGDNFDPQTSHVLPALIRKFHEARSTGGPVVLWGSGTPMREFLHVD
                     DAARAVRFVLELDEATLHGAAPDGMLNVGVGSDLSIRDLATLVADVVGYEGPVEYDRS
                     RPDGTPRKLVDVSRMRDLGWTAGIPLREGIEETWRWYVRNAR"
     gene            complement(9604..10671)
                     /gene="gmd"
                     /locus_tag="AC16YU_10730"
     CDS             complement(9604..10671)
                     /gene="gmd"
                     /locus_tag="AC16YU_10730"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6065743.1"
                     /translation="MASQKPRVALISGVTGQDGAYLAELLLAKGYVVHGIKRRASSFN
                     TQRVDHLYQDPHDPEVRFHLHYGDLTDATNLIRIVQEVRPDEIYNLGAQSHVQVSFET
                     PEYTANSDAVGVLRLLEAVRILGLGERTRFYQASTSELFGKVRETPQTEQTPFYPRSP
                     YGAAKLYGYWITVNYREAYGLFASNGILFNHESPVRGETFVTRKITRAAARIALGLQD
                     VLYLGNLDARRDWGHARDFVEGMWRILQHHEPDDFVLATGRTTSVRDLAEMAFAAAGL
                     ELEWQGGGVDEKAVDRRTGTVRIAVDPRYHRPTEVDLLVGDASKARRLLGWEPTCTLE
                     QMVEEMVDADLAAARKGEAGR"
     gene            complement(10676..12022)
                     /locus_tag="AC16YU_10735"
     CDS             complement(10676..12022)
                     /locus_tag="AC16YU_10735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR6065744.1"
                     /translation="MHIAVIGTGYVGLVTGTCFAETGFRVTCVDIDEEKVRTLQSGRS
                     TIYEAGLEPLIQRSVRERRLTFTTELAKTAVEADVLFLALPTPPGADGRADLSFVLRV
                     ADELGAVWAARGDRAYRLVVNKSTVPVGTADQVRQALEAHGLEAGRDFDVVSNPEFLR
                     EGVAVSDFMKPERVVVGTSSERAAEIMRSIYEPFVRQGNPILVMDERSSEMTKYAANA
                     FLAMKVSFINEVANLCEMTGANVDQVRVGIGLDSRIGNQFLYPGLGFGGSCFPKDVQA
                     LLRTAADHDYAFETLEAVLRVNRRQHDKLPRRIQAHFGGSLDGVPLAVWGLAFKAKTD
                     DIRESPAIAVVRGLLERGARLKAFDPEAMPNTKRLLGDRIGYATDMYDALDGARALVI
                     CTEWHEFRRPEWDEVIRRLPDRVVFDGRNLYRPSRMAELGFAYFSIGRPYTSPQNA"
     gene            12183..>13919
                     /locus_tag="AC16YU_10740"
     CDS             12183..>13919
                     /locus_tag="AC16YU_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6065745.1"
                     /translation="MHRYLLPALLLLAATPRVAGPGDGPILGFTAEGSAAQLDLEERF
                     AARLDPSNLDAWMRHMTSRPQHVGSPWGKANAEWVAGLFREWGFDAEVVRYDVLFPTP
                     VERAVELVAPARYVARLDEPELEGDATSRMRTDMLPPYNAYSADGDVEAELVYVNYGI
                     PDDYEELARMGIDVAGKIVIARDGGSWRGIKPKVAAEHGAVGCILYSDPRDDGYWQGD
                     VYPDGPYRMEWGTQRGSVADMPTYPGDPLTPFVGATPDAERLPVDEAPTLMRIPVLPI
                     SYGDALPLLEALGGRVAPPAWRGALPLTYHVGPGPARVHLRLRFDRDLAPAFNVIARL
                     EGADFPDEWIIRGNHRDAWVFGAADPASGHVAMMEEARVIGELAKEGHRPRRTIVYAS
                     WDAEEPGLLGSTEWVEHHADLLRERAVAYLNTDGSGRGFLGMGGSHALEPFINEVARA
                     VDDPQTAVSVWERARAKRRADGEKEADSEGDLRISPLGSGSDYTPFLQHLGIPTLNLG
                     FGGESGGGSYHSIYDSYDWYVRFGDPGFAYGVALAEVAGRATLRLANADVLPFRFRSL
                     AAHVARYVDEVTG"
BASE COUNT         1959 a         4830 c         4960 g         2170 t
ORIGIN      
        1 agcatgattc ctcgttcccc gagcatgcca tcgcgtattg cctggccgag gcggggatcg
       61 ggctggcgga cgtcgactac gtcgtctttt acgataagcc gttcctgaaa ttcgagcggc
      121 tgctcgagac ctacgtcgcg accgtgccgg gcggattccc ttcgttcctg aaggcgatgc
      181 cgctctggct caagcagaag ctctggattc cggacctcat tgaacgcgag ctgggctacg
      241 acgggccggt ggtctttccg gagcaccaca tgagccacgc ggcgagcgcg ttctacccgt
      301 ccccgttcga acgggcggcc gtcctgacca cggacggcgt gggcgagtgg gcgaccacgt
      361 ccgccggggt gggacacggc gccgacctcc ggatcctcga ggagcagcac ttcccgcact
      421 cgctgggact gctgtactcg gcgttcacgt acttctgcgg cttccgcgtg aactccggcg
      481 agtacaagct gatggggctt gcgccgtacg gagagccggc gtacgtggac cggatcctgg
      541 aggagctcgt cgagctgcgc gacgacggtt ccttccggct gaacatggag tacttcacgt
      601 acatggccgg cctcacgatg acgggtcgtc ggttcgcgcg cctcttcggc gggccgccgc
      661 gcggtgcgga ggcgccgatc acccggcgcg agatggacct cgcgtgctcg atccaggtcg
      721 tgacggagga ggccgtgatg cggatggcca ggcacgtcca tgagatcacg ggcgagcggg
      781 acctgtgcct ggccggaggc gttgcgctca actgcgtggc caacgggcgg ctgctgcgcg
      841 agggaccgtt cgagcgtctc tgggtgcagc cggccgccgg cgatgcgggc ggagcgctcg
      901 gggcggcgct cgcggcgtgg caccagcacc acggggcgcc gcgcaccgcc gcgagcccgg
      961 acgcgatgca gggcagctat ctcgggcccg cgtacggccg cgacgcgctc ctcgccttcc
     1021 tggagggcct cgggatcccg ttcgaagagg tcggcttcga ggcgctcccc gatcacgtgg
     1081 ccgggctgct cgcggagggc cgggtcgtcg ggtggttcca gggccgcatg gagttcggtc
     1141 cgcgcgccct gggcgcacgc agcatcctgg ccgatccgag gggccgcgac gtgcagcgca
     1201 cggtcaacct gaagatcaag ttccgggaga gcttccgccc cttcgcgccg agcgtcgccc
     1261 gggaccggct cgccggctgg ttcgacctgg acgcggactc gccctacatg ctgctcgtgg
     1321 cgccggtccg agacgcgcgc atcgagggcg tggggctcga ccgcctgaag ggcatcgact
     1381 cgccgatacc ggccgtgacg cacgtcgacg gatccgcgag gatccagacc gtgcaccgcg
     1441 ggacgaatcc cctgtaccat cggcttctcg aggcgttcga ggagctgacc gggtgtccgg
     1501 tcctcgtgaa caccagcttc aacgtccgcg gcgagcccat cgtgtgcacg cccggggatg
     1561 cctggcgctg tttccgccgc acgcacctgg acgtgctcgt gctcggaccc ttcgtggtcc
     1621 ggcgcgaccg gatcccggac gccgacgcgg aggtcatcga agccgaagac gccgcccgac
     1681 tctatggact cgactgagcc tctcgccacg ccgtccgtcc ccgcggaagg ctgggacgac
     1741 cgcaccctga ccgtgggcga agtgtggcgc tacctggcgc gcatcctgcc cctcgtcgtc
     1801 gtcgtcacga tcggcgtgca cgcgctggcc tggggcgcgc ccgggtacgg agacgacctg
     1861 ctcgtcggcg gcccctggat agggatcctg gtctacctcc tgagcgtcgt cgtgcacgag
     1921 gcccttcacg tcgcggcgat ggtggcgttc ggcgtgccgg tccggaccat ccgcgtgggc
     1981 tcccggctga gggagggggt ggtctacgtg catgccggtc gcgatctcac ggtcgggccc
     2041 taccgggtgg tgctcctcct gccggccctg gtgctcggcg tcgtgccgac cgtggccggc
     2101 ctggtcgccg gctcgtgggc gtggaccctg tacgggttcg tgatgctgat ctcggccgcc
     2161 ggggatttcg cggtcatcga ccggcttcgc ggacttcccc ccgggaccgt ggtccgcgac
     2221 caccccgaga aagtgggctg ccaggtgcgc cttccggagc ccgccctgcc ctgagatccg
     2281 ggcgtggcgc aatggacggg catggttacc ttgcgcggaa gcccttccgc atccgatcgg
     2341 tccgcacatg gccacgcgca gagagttctt cggcaccatc ggtcgacccg ccgccgcggc
     2401 gctggcgacc gcgaccttcc atccggcggc cgcttcccgg ctattcgacg tgctcgcgga
     2461 gtaccggggc acgccctcgg aggcggcgag cgacgaggag ctctggcgcg aggtgcagca
     2521 ggcgttcacc gtcgaccgga cgctcgtgaa cctgaacaac ggcggggtga gcccggcgcc
     2581 cgccgtggtg caggaggcga tgaagcgcca cctggactac tcgaacgagg cccccgtcta
     2641 caccatgtgg cgcatcctgg agccgcagcg cgaaggggtg cggcagcgcc tggcccgggc
     2701 gttcggctgc gacccggagg agatcgccgt cgtgcgcaac gcctcggaag gcctccagac
     2761 gtgccagctg ggcatcgatt tcgaaccggg cgacgaggtg ctgaccacga cgcacgacta
     2821 tcctcgcatg atcacgacgt tccagcagcg ggagcggcgt gacggcatcc gtctggtcca
     2881 gttcccgctg cccatcccgg ccgaggatcc cgccgaagtg gtccgcctgt tcgagtcgca
     2941 catcacgccg cgcacgaagg cgatcctgat gtgccacatc gtcaacatca cgggacagat
     3001 cctgcccgtg aaggcggtcg tgcagatggc ccgtgcgcgg ggcattccgg tgatcgtcga
     3061 tggcgcgcac tccttcgccc acttccactt cacgcaggcg gacctcgact gcgactattt
     3121 ctcgacgagc ctgcacaaat ggctcttcgc gccgcacggg accggattcc tgtacgtgcg
     3181 gaaggagcgc attcccgacc tctggccgat gatggccgcc cccgagacgc tcgacggaga
     3241 catccggaag ttcgaggaga tcggcacgca ccccgccgcc aacttcctcg cgatcggcga
     3301 ggccctgacc ttccaccagg gcataggccc cgagcgcaag gaggcgcggc ttcggtacct
     3361 tagggactac tgggccacgc gcctcctgga gcacgaccgc gtccggctgc acaccagcct
     3421 gaagcccgag ttctcctgca cgatcgccaa cgtgcagatc gagggagtgg cgagcgtcgc
     3481 gctcggcaac tacctgtggg accggcaccg catcatcgtg acgccgatcg tccatcccga
     3541 cttcgagggt ctgcgcatca cccccaacgt gtacaccacg ctcgaggagc tggaccggtt
     3601 cgtggacgcg atggagcacg tcgtccggaa cgggcttccc gagccgtacg ggacgccctg
     3661 agcgcgggat ccgaaccatg acggaccccg tcgtcatcat cgggggagga atcgtcggcc
     3721 tgtcgaccgc ctggcacctg tcccggcatc cggaggccgg tcccgtcctt gtactcgaga
     3781 aggaggacga ggtcggcctt caccagacgg gccgcaattc gggcgtcatc cactccggca
     3841 tctattaccg gccgggcacg ctcaaggcgc gcctttgccg cgacgggcgg cagcgcctgg
     3901 ccgaattctg tgaggaacgg gggatcccgt acgagttctg cggcaaggtg atcgtggccg
     3961 tcgacgacgc ggaggccggg aggctgcccg ggctcctgga gcgggggcgg gcaaacgggg
     4021 tttcgtgccg cgccatcgat cgggcggagc ttcgcgagcg cgagccgcac gtggaggggc
     4081 gtgcggccat cctggtcgac gacaccggaa tcgtggattt cctggaggtg acgcgtcacc
     4141 tcgcggcgct cctcgagtcg tcggggcacg aggtccgtcg gcggagcggc gtccgggcga
     4201 tcgggcagga gggcggcggc taccggctgg acaccacggc gggtcccgtg cgggcacgat
     4261 tcctcgtgaa ctgcgccggg ctgcatgccg accgtgtggc gcgcatggcg ggcgtggacc
     4321 cgggggtgcg tctcgtaccc ttccggggac agtactacga gctgcccggc gaggggcgcc
     4381 gcttctgccg gcacctggtg taccccgtgc cggatccggc ctacccgttc ctgggcgtgc
     4441 acctgacgcg tcggatcgac ggccgcgtgg agtgtggccc caacgcggtg ccggccatgg
     4501 cccgcgaggg ttaccggttc cgcgatgtga atctcggcga cctccgcgag atcgtggcat
     4561 tcctgggctt ccggcgcctg gcgctcaggc actggaggat gggctcggcc gagatcgccc
     4621 gggcggtgtc gacgcgccgg ttcgtggcct cgctacggcg cctcgtgccc gggatcgagc
     4681 ggggcgacct gagcgcggct ccctccggtg tccgggcgca ggcgctgacg cccgggggcg
     4741 agctggcgga cgatttcgtc atccgggacg cccggggtgc ggttcacgtc gtgaacgctc
     4801 cctcgcccgc cgcgaccgcg agcctcgcca tcggggcgtt tctggcgcag cacgtcctgg
     4861 gccggatcgc tgcctgaggc gcacacaagg cggtccggat ccaaaaggac gcccggcctt
     4921 gaagccgggc gttcagcgac ggggccctcc cgagggcgtg tcagatccgg actgttcata
     4981 cggtcgcgag gcgaccgcgg ccggatcggc tcctgccgtc cgacacgttg tttcggtgct
     5041 ggtctgtcgg cccacgcagg ggagcgtatc cgtcgccgac taacgtaata cgaatcgatg
     5101 ggagcgtctg caaggcactt ctggggaatg ccatcgggaa ttcccccgta cccgtgcgcc
     5161 atcggcggcg caaggggcac ccgctctttt ccgacgcgcg gcgcaggcct ttccccatgg
     5221 gggtgcaggg ggcgctgcgc gcggaattcc gtggaatcca ctccttgaac ggggagccat
     5281 cgtgacccat attgcccgtc cgccgcttcg agacagtgct gccgatcccc ccatgagcag
     5341 gcccgcttcc gtccatccgt ccgagaccct gtactcgagc tgccgatacg tcgacgacgt
     5401 aaggctgtac atgggtcggg ccgtgctcac cccggacgag atccgggtgc acgggtgggc
     5461 cgggacgcac cacgtctcca tgcagatacc gctcaaggac gtgaccggcc tggcgcgcag
     5521 cgcgcagggg cgcgggccga tgctcgtcct ggacacggcg gacggccgga cggtcgccct
     5581 gggaatatcc gcgccgggcc tttggcgatg ggcggtgagg cagcgccttc ccgccggaca
     5641 tccccttgcg ccctcggccg acgaaccggt gcccgacgcg cccgctccgc ggcgggagtc
     5701 ccacgcattc ctgctccggg tcgcctacct ggccggggac cgcgtccccg ccacggcgga
     5761 tggcgacatc cggtggcggc cgctgggcga gctcacgtcc gcgtgatcgc gatttactcg
     5821 tccgggtgat cctcgggccg cggcggcggc gcgcccgggt cgaagagcat gcccgtgcac
     5881 aggcagtgct gcttcctagt ccgggtcagg atatcgacgt tcgggcagct ctggcagccc
     5941 cgccagaaca cgtcgtcctg ggtgagctcc gagaaggtca ccggccggta cccgaggtcc
     6001 gcgttgatct tcatgacggc caggctcgtg gtgatgccga agagctttgc gtcggggtag
     6061 cgctcccggg acaggcggaa ggccctccgc ttgatgcgcc gcgccagtcc cagcttccgg
     6121 aattcgggca cgacgatgag tcccgagttg gccacgtacc ggccgtgctc ccaggtttcg
     6181 atgtagcaga agccggccag gcggtcgtcc gaaagcgcga tgatggcctt gccctcctcc
     6241 atcttgccgc ggatgtactc cggcgtccgc ttggcgatac cggtcccccg gatccgggcg
     6301 gcatcggcca tctcggcaca gatctgctcg gcgtacggga agtcatccgg tccggcgacg
     6361 cggaccgtga ccacgggaac gggatcgggc aggttgcccg cagccgccgg aagagcgctc
     6421 atacgcgtat tgtcctgggt ataagtgcga tctaatgcga tcttgccggc gaagttcgcg
     6481 gccggttccg cgcccgcacg aaatgcccgc gggcacccgg gcgaacggtc cggtcggcgt
     6541 atcttgcagc ccgtcgaaac ccgtcgagtc ctcgcgacca tgatccgcta ccttaccgcc
     6601 ggagagtccc acggcgaggc cctggtgggc atcgtcgagg gcatgccggc cggagttccg
     6661 gtcaccgcgg cggacctcga cgagcatctc gcccggcgct ggctcggatt cggccgcgga
     6721 gggcgagccc gcatcgagaa ggatcacgtc cacatctact ccggcattcg cttcggccgg
     6781 acgatgggca gcccgatcgc cctgcgcatc gacaacgcgg cctacctgcg cgaccgctcc
     6841 ggatggcccg acatcatggc gatcgaggag acgggtgatc ccgtcgagaa ggtcacgctg
     6901 ccccgtcccg ggcacgcgga cctggccggc gtccagaagt acggcttcga cgacatccgc
     6961 ccggtgatcg accggtccag cgcccgggag acggccatgc gcgtggcctg ctgctccgtg
     7021 gcccgtcgcc tgctcctgga attcggcatc gaggtcggct cgcacgtgct ccgcatcggg
     7081 gaagtcgcgg cggatggaga ggccacgggc gccacggtcc ggcgcctgct ggaggagggg
     7141 ggagcctcgg ccctgtacgc ggccgcggac cgggacgaag tgcggatgct ggacgcggat
     7201 gcgtccgcgc gcgcgatcga ccgcatcgtg aaggccaagg aggacggcga ttcgctgggc
     7261 ggcgtctacg aggtggtggt gaccggcgtg cccccgggcc tcggctcgta cgtgcactgg
     7321 gaccgtcgcc tggacgggca gctcgcgcag gccatccttt cgatccaggc acagaaggcg
     7381 gtcgaggtgg gggacggtgt cgccgcggcg agtctccagg gctcccgcgt gcacgatccg
     7441 atacgccgcg acggcgggcg tttcacgcgc gagtccaacc gggccggcgg cgtggagggc
     7501 ggcgtgacga cgggcatgcc ggtcgtggtc cggggcttca tgaagcccat tcccacgttg
     7561 atccggccgc tcggcacggt ggacatcgcg accggtgagg cgcagccgac gcgctatgag
     7621 cgcagcgacg tcacgtccgt gccggccgcc tcgacggtgg ccgaggcgac cgtcgccctg
     7681 gtcatcgcca atgccttcct ggagaagttc ggtggcgaca gcctggacga gatccggggc
     7741 cgcttcgagc gcgaggtgcg gggctccgga tgagcgcgcg cggcgggagg ccgcgaccgg
     7801 atcagcggcc gaaccgcatg tctccgcgga atccgcgtct cacgccggtc cgtccgtcca
     7861 cggcggcggg gcccgcgccc atcacgccgg cgagggcatc atggatacgc agcacccgga
     7921 cctgctcgtc cgagaggtcc agggctttcc gcatgccctc gatccgggcg tcccggagcc
     7981 tggccatggc ctccgggacc cgggccggcc gctcggcgac gcgatgcaca cggcgacccg
     8041 cctggccggc tccgcgacgg aagccgtcgg ccatgcctct gcggtaaatc ccggcctcgc
     8101 gcgaggcctc gaacagcttg tcgacctggg cgggcgtgag atccctggcg agcacggccg
     8161 ccacggtcca gccgtcgccg gcccggcccg tctcctcgtc cgcctgcatg acctcgccga
     8221 cggccgcctt ctgggcgtcg gtcagctcga gtcgttcgga gagtcgggtg agggccgcgt
     8281 cgcgccgcgc ctcctgtcgc tcgattcggg tggcctgtct tgcctcccgc attgccgcgc
     8341 gatcgtccgt ccggtcctgg ccggatgccg gggccgccgc gaggagcagt gcggcggcca
     8401 gcggtgccgc cctgcgaaga atgattcttc ctttcatgtc gtgcctccgt ggagggtctg
     8461 ttggatgatt cgtacctgca ccacggttaa cggggcggcc tgccccgggt tgtcacgagc
     8521 cggtcacggg atggcggcga tttggccccc gaacggtagc ggcctcgtga cgggtggcga
     8581 cgcgaaccgc cggaccaggt cgcccactga cgccacgtcc ggagcacgca gcgccggggt
     8641 ccggatcgcg tcaacgcgcg ttccggacgt accagcgcca ggtttcctca atgccttcgc
     8701 gcagcggaat gccggcggtc catcccaggt cccgcatccg ggaaacgtcc acgagcttgc
     8761 gaggcgtgcc gtccggcctg gaccggtcgt actccacggg cccttcgtag ccgacgacgt
     8821 ccgccacgag cgtcgccagg tcccggatcg acaggtcgct gccgacgccc acgttcagca
     8881 tgccgtccgg ggcggccccg tgaagcgtcg cctcgtccag ctccagcacg aagcgcacgg
     8941 cgcgggccgc gtcgtccaca tgcaggaact cgcgcatggg cgtgcccgat ccccagagca
     9001 ccaccggtcc gcccgtcgag cgggcctcgt ggaacttgcg gatgagcgcc ggaagcacgt
     9061 ggctcgtctg cgggtcgaag ttgtcgcccg gcccgtacag gttggtcggc atcaggctca
     9121 cgaagtccgc gccgtgctgg cgacggagcg cctggcacag cttgagcccc gcgatcttcg
     9181 cgaccgcata ccactcgttg gtgggctcga gcgggccggt gagcagcgac tcctcgcgca
     9241 gaggctgcgg tgcgagtctc ggatagatgc acgtcgagcc caggaacacc agccggcgga
     9301 cccccgcgag gtgggccgcc cggatcacgc tcgtctggat cgccaggttg tcgtacagga
     9361 agtccgcggg ccaggtggcg ttcgcgagga tgccgccgac gcgcgccgcg gcgagcacca
     9421 cgaactcggg tcgctcctcg tcgaagaagc gggcgacggc cgcctggtcg cgaaggtcga
     9481 gatcccccct ggcgcgggtc acgaggttcc cgtagcccgc cgcgccaaga gcacggacca
     9541 cggccgagcc gaccaggccc cggtggccgg cgacgaagat ccgggaatcc gggtcggaga
     9601 gtctcatcgg cctgcctccc ccttccgagc ggcagcgagg tccgcgtcga ccatctcctc
     9661 caccatctgc tcgagcgtgc acgtcggctc ccagccgagc agccggcggg ccttggacgc
     9721 gtcgccgacc aggaggtcca cctccgtcgg gcgatggtag cggggatcga cggcgatccg
     9781 caccgtgccg gtccggcggt cgaccgcctt ctcgtccacg cccccgccct gccactccag
     9841 ttccaggccg gccgcggcga aagccatctc ggccaggtcg cgtacggagg tcgtccgccc
     9901 ggtcgccagc acgaagtcgt ccggctcgtg gtgctggagg atgcgccaca tgccctcgac
     9961 gaagtcccgc gcgtggcccc agtcccgccg cgcgtccagg ttgcccaggt acagcacgtc
    10021 ctgcagcccg agcgcgatgc gcgcggctgc ccgcgtgatc tttcgcgtga cgaacgtctc
    10081 gccgcggacg gggctctcgt ggttgaagag gatgccgttg ctcgcgaaga ggccgtacgc
    10141 ctcccggtag ttcaccgtga tccagtagcc gtagagcttg gccgcgccgt aggggctgcg
    10201 cggatagaac ggggtctgct ccgtctgggg tgtctcgcgc accttgccga agagctcgga
    10261 cgtcgacgcc tggtagaacc gcgtcctctc gcccagcccc aggatgcgca cggcctcgag
    10321 caggcgcagc acgcccaccg cgtcggagtt cgccgtgtac tcgggcgtct cgaacgaaac
    10381 ctggacgtgg ctctgcgcac cgaggttgta gatctcgtcg ggccggacct cctggacgat
    10441 ccggatcagg ttcgtggcgt cggtcaggtc gccgtaatgc aggtggaacc ggacttccgg
    10501 gtcatgcgga tcctggtaca ggtgatccac gcgctgcgtg ttgaaggagc tcgcgcggcg
    10561 cttgatgccg tgcaccacgt agcccttggc gagcaggagc tcggcgaggt aggcgccgtc
    10621 ctggcccgtg actccggaga tgagcgcgac ccggggtttc tggctggcca tgcgttcagg
    10681 cgttctgggg ggacgtgtag ggacggccga tggagaagta ggcgaagccg agctcggcca
    10741 tgcgggacgg ccggtacagg ttccgtccgt cgaagacgac gcggtcgggg agacgccgga
    10801 tcacttcgtc ccactcgggg cggcggaact cgtgccactc cgtgcagatg acgagcgcgc
    10861 gggccccgtc gagcgcgtcg tacatgtcgg tcgcgtaccc gatgcggtca ccgagcagcc
    10921 gcttcgtgtt cggcatcgcc tccgggtcga aggccttcag ccgggctccg cgctcgagca
    10981 ggccccgcac gacggcgatg gccggcgact cgcggatatc gtcggtcttc gccttgaagg
    11041 cgagccccca tacggccagt ggcacgccgt cgagcgatcc tccgaagtgc gcctggatgc
    11101 ggcgaggcag cttgtcgtgc tgccgccgat tcacgcggag caccgcctcg agcgtttcga
    11161 acgcatagtc gtggtcggcc gccgtgcgga gcagcgcctg gacgtccttc gggaagcagc
    11221 tcccgccgaa tcccaggccc ggatacagga actggttgcc gatgcggctg tccaggccga
    11281 tgcccacgcg cacctggtcc acgttcgctc cggtcatctc gcaaaggttc gcgacctcgt
    11341 tgatgaacga caccttcatg gcaaggaagg cattcgcggc gtacttcgtc atctcggacg
    11401 agcgctcgtc catcacgagg atggggttac cctgccgcac gaacggctcg tagatggagc
    11461 gcatgatctc cgccgcccgc tcgctcgacg tgccgacgac cacgcgctcg ggcttcatga
    11521 agtccgagac cgccacgccc tcgcggagga attccggatt ggagacgacg tcgaaatccc
    11581 ggcctgcctc gaggccgtgg gcctcgagcg cctggcggac ctggtcggcc gtgcccaccg
    11641 ggacggtgct cttgttcacg acgaggcggt acgcacggtc gccccgggcc gcccagacgg
    11701 cgcccagttc gtccgcgacg cggagcacga acgacaggtc ggcccggccg tcggctccgg
    11761 gcggggtggg cagcgccagg aacagcacgt cggcctcgac cgcggtcttc gcgagctcgg
    11821 tggtgaacgt caggcgccgc tcgcggacgg agcgctggat gagcggctcg agccccgcct
    11881 cgtagatggt cgaccgcccg gactggagcg tgcggacctt ctcctcgtcg atgtcgacgc
    11941 aggtgacgcg gaatccggtt tcggcgaagc acgtgcccgt gaccaggccc acgtacccgg
    12001 tgccgatgac ggcgatatgc atggtgttcg gtggcggagt cgtattgcga gccgtcaaag
    12061 ataggccgcg ggccggacgt cagccgttcg atccggcgtt gtccgtcccg gttttcctcc
    12121 gtccggcgtg gccttcgacg ccggtccggg gtagcttccc tcgtccagat cgcccgaaag
    12181 ccatgcatcg gtatctgctc cccgccctgc ttctcctcgc cgccacgccc cgcgtcgccg
    12241 gtcccggtga cggaccgatc ctgggcttca cggccgaggg gtcggccgcc cagctcgacc
    12301 tcgaggagcg cttcgccgcg cggctggacc cgtcgaacct cgacgcgtgg atgcggcaca
    12361 tgacctctcg gccgcagcac gtcggctcgc catggggcaa ggcgaacgcg gagtgggtcg
    12421 ccgggctctt ccgcgagtgg ggcttcgacg cggaggtcgt gcgctacgac gtgctttttc
    12481 cgacgcccgt cgagcgggcc gtcgagctcg tggcccccgc caggtacgtg gcccggctcg
    12541 acgagccgga gctcgaaggc gatgccacgt cgcggatgag gacggacatg ctgccgccct
    12601 acaacgcgta ctcggccgac ggtgacgtcg aggcggagct cgtgtacgtc aactacggga
    12661 ttcccgacga ctacgaggag ctggcccgca tgggcatcga cgtggccggc aagatcgtca
    12721 tcgcgcgcga cggcggctcc tggcgcggca tcaagccgaa ggtcgcggcc gagcatggcg
    12781 cggtcggctg catcctgtac tccgaccccc gggacgacgg ctactggcag ggcgatgtgt
    12841 acccggacgg cccgtaccga atggaatggg gcacccagcg gggcagcgtc gccgacatgc
    12901 ccacctatcc cggcgatccg ctgaccccgt tcgtgggcgc gacgcccgac gcggagcgcc
    12961 tgcccgtgga cgaggcgcct acgctgatga ggattcccgt cctgcccatc tcgtacggcg
    13021 acgcgctgcc gcttctggag gctctcggcg gacgggtggc cccgccggca tggcgcggcg
    13081 cgcttcccct cacgtaccac gtgggccccg gcccggcccg cgtgcatctc cggctgcggt
    13141 tcgatcgcga cctggcgccc gccttcaacg tgatcgcgcg cctcgagggc gccgactttc
    13201 ccgacgagtg gatcatccgg ggcaatcacc gggacgcctg ggtgttcggg gcggcggatc
    13261 cggccagcgg gcacgtcgcg atgatggagg aggcccgcgt gatcggggag ctggcgaagg
    13321 agggccaccg gccccggcgt acgatcgtgt atgcgagctg ggatgcggag gagccggggc
    13381 tcctgggctc caccgagtgg gtggagcacc acgcggacct gcttcgcgag cgtgccgtcg
    13441 cctacctgaa caccgacggc agcgggcggg gcttcctggg catgggaggg tcgcatgccc
    13501 tcgagccgtt catcaacgag gtggcccgcg ccgtcgacga cccgcagacg gccgtgagcg
    13561 tgtgggagcg cgcgcgggcg aagcgccgcg cagacggcga gaaggaggcg gattccgagg
    13621 gcgacctgcg catcagcccg ctcggctcgg ggtcggacta cacgccgttc ctgcagcacc
    13681 tgggcattcc cacgctgaat ctcgggttcg ggggcgagag cggcggcgga tcctaccatt
    13741 ccatctacga ctcgtacgac tggtatgtcc gcttcgggga ccccggcttc gcctacggcg
    13801 tcgccctggc cgaggtcgcg ggccgcgcga ccctccggct ggccaacgcc gacgtgctgc
    13861 cgttccgttt ccgctcgctc gcggcgcacg tggcgcgcta cgtggacgag gtcaccggg
//
LOCUS       DBNHQZ010000394         5170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1743784, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000394 DBNHQZ010000000
VERSION     DBNHQZ010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1743784"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16YU_10745"
     CDS             complement(<1..770)
                     /locus_tag="AC16YU_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6065746.1"
                     /translation="MRPLAPILLLAACLAPRAQAQIDDAVLAIEPRVVEWRRDFHRNP
                     ELSNREFRTAGIVAEHLRGLGLEVSTGVAHTGVVGVLRGGRPGPVVALRADMDALPVP
                     ERNDLPFRSTAVGEYRGEEVPVMHACGHDTHVAMLMAVAEILAGRRDDLPGTVKFIFQ
                     PAEEGAPPGERGGAEVMVEEGVLENPRVDAVFGLHIDAQREVGTIGYRPRGAMAGVQD
                     FRIMVHGRQAHGSTPWHGIDPIVTAAQIVGSLQTVVSR"
     gene            complement(829..1260)
                     /locus_tag="AC16YU_10750"
     CDS             complement(829..1260)
                     /locus_tag="AC16YU_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR6065747.1"
                     /translation="MHSRSVDIGLLVLRLGFGLAFIWFHGWDKITAGPERWESLGGNM
                     ANYGITFWPTLWGFLASVAESVAAFLIAVGFLFRPASFLLAFTMLTASIFHIVSGQGS
                     PAHSIKNLFVAAGVGIAGPGRYSVDAWIASSRAKRAPGTKA"
     gene            complement(1340..1876)
                     /locus_tag="AC16YU_10755"
     CDS             complement(1340..1876)
                     /locus_tag="AC16YU_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065748.1"
                     /translation="MNAPSFRRLAGPAVLLFALAWMGCEGSPVPLSDPPSVPVDPAYL
                     GTWEMASPEEEAGDLAVVHRFNDFEYLVEYREVSRADDGTTVFDEVQLLRMFVTDVDG
                     RPFLNVKGIGGDDEDWFFLEIESVDATQARLRTVSDDLYEDLDDTATPESVLAALRGM
                     IRSPDGYGDSAEFRRVED"
     gene            complement(1948..3300)
                     /locus_tag="AC16YU_10760"
     CDS             complement(1948..3300)
                     /locus_tag="AC16YU_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR6065749.1"
                     /translation="MRRIIEPFRIKVVEPIRFTEPEEREQVLRRAGYNLFKVPSDAVI
                     IDLLTDSGTGAMSAAQWAGIMRGDESYAGSPSFERFEAAVRDITGFRYVIPTHQGRAA
                     ERILATTMIRPGDVVPNNTHFDTTRANVEAAGATALDFRCAESLDLFTPSDFKGNIDL
                     DGLERCLAANEGRVPFAMITVTNNGGGGQPVSMANIRAASELCRAAGVPFYLDACRFA
                     ENAFFIKKREPGYADRSLIEIAREMFSYADGCTMSAKKDGMANIGGFLCSNDAQIARQ
                     EEELLILTEGFPTYGGLAGRDLEAVAIGLYEALDLDYQEYRASSTAYLGEHIAEEGIP
                     IVQPPGGHAIYVDAGSMLPHIPASEFPGQALVIALYREGGIRAVEIGSVMFGGGGAQV
                     PFELVRLAIPRRVYTKSQIDYVVDVFRDLSRMRDRLRGVRIVREPARLRHFTAEFELL
                     "
     gene            complement(3311..>5170)
                     /locus_tag="AC16YU_10765"
     CDS             complement(3311..>5170)
                     /locus_tag="AC16YU_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6065750.1"
                     /translation="EYAGPLRETSFVAEKAREYGLSDVEVIPYEGGPEWDGSVGELWV
                     TSPVLRKVIDFRDISAALAPGSVTGTYEGPLVWIENASDPEAYAGRDVTGAIVLTRAS
                     TRSAFTAATERGALGVVNMAAQRPYTMPETIQWSSIGEPEAGFAFNLPAIIVDDLREL
                     QIRNDEVTVRAVVEAEWRRADNEVVTAVIPGDGSSDEWVWYTAHLFEGIQKQGAADDG
                     SGSVLILETGRALVEAMARGTVRRPARSIRFLWMDEFTGTYAFIEDRADEFSRVVANV
                     NIDMAGQNVTLNNNATRLYRMPYSRIHFLGDVAQEFFEWVGTTNIERIHERGGGYKYS
                     YPILDPYGTRDAWRYVVEPYYGSSDHVVFNDMGVPGVMFNHWPDMVYHTSHDRPAMMD
                     ATQMKRVAFIAAATGLVVAGSPDVSVPQVAAEAFGRGEARIAVDLRTWTRHLASLPAD
                     ELAAGYGAASAAVAQWHAREARNIRTVLQLTEGSTSSVPAVDAATIELLAAEMEARTA
                     ASARALDIAWEGLTSRLRVSAERPEPTPAEVRAAAIVPKPTGSTVRVRIRAEGLPGFT
                     AMEARNFADGTRSLVEIRDAVNAEYALEYGPVTLAAVEAYLRQLADAGILVLE"
BASE COUNT          789 a         1804 c         1790 g          787 t
ORIGIN      
        1 ctccgcgaga ccacggtctg caggctgccg acgatctggg cggccgtaac gatcgggtcg
       61 atgccgtgcc atggcgtgga tccgtgcgcc tgccgaccgt gcaccatgat ccggaagtcc
      121 tggacgcccg ccatcgctcc gcgcggccgg tagccgatcg tgcccacctc gcgctgggcg
      181 tcgatgtgca gcccgaagac ggcgtccaca cgcggattct cgagcacgcc ctcctcgacc
      241 atgacttccg cgccgccccg ctcccctgga ggcgctccct cctcggccgg ctggaagatg
      301 aacttgacgg ttcccggcag gtcgtcgcgc ctgccggcca ggatctcggc gacggccatc
      361 agcatggcca cgtgcgtgtc gtgcccgcac gcgtgcatca ccggcacttc ctcgccccgg
      421 tactcgccga ccgcggtgga gcggaagggc aaatcgttcc tttcaggcac cgggagcgcg
      481 tccatgtccg ctcgcagcgc gacgaccggc ccgggtcttc cgccccggag gacgccgacc
      541 actccggtat gcgcgacgcc ggtcgagacc tcgagcccca ggccgcgcag gtgctccgcg
      601 acgatgccgg cggtccggaa ctcgcggttc gagagctcgg ggttccggtg gaagtcacgt
      661 cgccactcga cgacgcgcgg ctcgatggcc aggaccgcgt cgtcgatctg ggcctgcgca
      721 cgcggggcca ggcaggccgc gaggaggagg atcggagcga gagggcgcat cggcgggcgt
      781 cggattgtga agggtccaag aaggtacgcc cgccgcccgt accacgcctc aggctttcgt
      841 gcccggtgcc cgtttcgccc tcgaggaggc gatccacgcg tccacgctgt accggccggg
      901 tcccgcgatg ccgacgccgg cggccacgaa caggttcttg atcgagtggg cgggcgaacc
      961 ttggccggac acgatgtgga agatcgaggc ggtcagcatc gtgaacgcca aaaggaagct
     1021 ggccggccgg aacaggaagc cgaccgcgat caggaacgcc gccacggact ccgcgacgga
     1081 ggcgagaaat ccccacagcg tgggccagaa cgtgatgccg tagttggcca tgttgccgcc
     1141 gagcgactcc cagcgttcgg gccctgccgt gatcttgtcc cagccgtgga accagatgaa
     1201 ggcgagcccg aagccgaggc gcaggaccag gaggccgata tctacggatc gggagtgcat
     1261 gacggccgcg cggggtgatg aagggaaccc gaatatacgg gcggcggctc tgccggccat
     1321 gtggccggcg tgtccccggt cagtcctcga cgcgccggaa ctcggccgag tcgccgtagc
     1381 cgtcgggcga tcggatcatc ccgcgcaggg ccgcgagcac gctctccggg gtggccgtat
     1441 catcgagatc ctcgtacagg tcgtcagaaa cggtccggag ccgggcctgc gtcgcgtcga
     1501 cggactcgat ctcgaggaag aaccagtcct catcgtcgcc tccgatgccc ttgacgttca
     1561 ggaagggccg gccgtccacg tcggtgacga acatccgcag cagctgcacc tcgtcgaaga
     1621 ccgtggtgcc gtcgtccgcg cgagagacct cccggtactc gaccaggtac tcgaagtcgt
     1681 tgaatcggtg gacgaccgcg aggtcgcccg cctcctcctc gggcgaagcc atctcccagg
     1741 tccccaggta cgcggggtcc accggcaccg agggcgggtc ggagagcggg acgggcgagc
     1801 cctcgcagcc catccaggcc agggcgaaga gaaggacggc ggggccggca agccgccgga
     1861 aagagggtgc attcattgtt tcgagaggtc atccgggggg atcgttcacc cgaataagcc
     1921 gcagccgccc gcccgggggt aacgccgtca gaggagctcg aactcggccg tgaagtgccg
     1981 gagccgcgcg ggctctcgga cgatgcgcac gccccgcagc cggtcgcgca tcctggaaag
     2041 gtcgcgaaag acgtcgacga cgtaatcgat ctggctcttc gtgtagaccc gccgcgggat
     2101 cgcgagccgc acgagctcga aagggacctg cgcgcccccg ccgccgaaca tgaccgagcc
     2161 gatctcgacg gcccggatgc cgccttcccg gtagagcgct atgaccagcg cctgtccggg
     2221 aaactcggag gcggggatgt gcggaagcat ggatccggcg tccacgtaga tcgcgtgacc
     2281 gccgggcggc tgcacgatgg gaatgccttc ctctgcgatg tgctcgccca ggtacgcggt
     2341 cgacgatgcg cggtactcct ggtagtccag gtccagggct tcgtacagtc cgatggcgac
     2401 ggcttccagg tcgcggccgg cgagccctcc gtaggtcgga aagccctcgg tgaggatgag
     2461 cagctcctcc tcctgccggg cgatctgcgc gtcgttcgag cagaggaaac cgccgatgtt
     2521 cgccatgccg tccttcttgg cgctcatcgt gcagccgtcc gcgtaggaga acatctcgcg
     2581 cgcgatctcg atcaggctcc ggtccgcgta tcccggctcc cgcttcttga tgaagaaggc
     2641 gttctccgcg aaccggcacg cgtccaggta gaacgggacg cccgccgccc ggcacagctc
     2701 cgaggccgcg cggatgttcg ccatggacac gggctggccg ccgccgccgt tgttcgtcac
     2761 ggtgatcatc gcgaagggca cccgcccctc gttcgcggcc aggcagcgct cgagcccgtc
     2821 caggtcgatg tttcccttga agtcgctggg cgtgaagagg tccagcgact cggcgcaccg
     2881 gaagtccagg gccgtcgcgc cggccgcctc gacgttggcg cgcgtcgtgt cgaaatgcgt
     2941 gttgttcggc accacgtccc cgggacggat catggtcgtc gccaggatgc gctcggccgc
     3001 acgcccctgg tgggtcggga tcacgtaccg gaagcccgtg atgtccctga cggcggcctc
     3061 gaaccgctcg aaggaggggc tgcccgcgta cgactcgtcc ccgcgcatga tgccggccca
     3121 ctgcgcggcg ctcatcgccc cggtgcccga atccgtgagc aggtcgatga tgacggcgtc
     3181 ggacggcacc ttgaacaggt tgtacccggc gcgccggagg acctgctccc gctcctccgg
     3241 ctccgtgaag cggatgggct cgacgacctt gatgcggaac ggctcgatga tgcggcgcat
     3301 gccggcaaag ctactccagc acgaggattc ctgcgtccgc caactgccgg aggtaggcct
     3361 cgaccgccgc gagcgtcacc gggccgtact cgagggcgta ctcggcgttc accgcgtcgc
     3421 ggatctcgac gaggctccgc gtcccgtccg cgaaattgcg ggcctccatc gcggtgaacc
     3481 cgggcagccc ctcggcccgg atgcgaacgc gcacggtcga gcccgtcggt ttcggcacga
     3541 tggccgcggc ccggacctcg gccggcgtcg gctcgggacg ctccgcgctc acgcgcagcc
     3601 tggaggtgag tccctcccag gcgatgtcga gcgcccgcgc gctcgccgcg gtccgcgcct
     3661 ccatctcggc cgcgagcagt tcgatggtgg ccgcgtcgac cgccggcacg gacgaggtgg
     3721 agccttccgt gagctggagc accgtccgga tgttccgggc ctcccgggcg tgccactggg
     3781 cgacggccgc ggaggccgct ccgtacccgg ctgcgagctc atccgccggc agcgaggcga
     3841 ggtggcgcgt ccacgtacgc aggtcgacgg cgatgcgcgc ctcgccccgg ccgaacgcct
     3901 cggccgccac ctgcggcacg gacacgtcgg ggctgccagc aacgaccagg ccggtggccg
     3961 cggcgatgaa ggccacgcgc ttcatctggg tagcgtccat catggccggg cgatcgtggc
     4021 tcgtgtggta caccatgtcc ggccagtggt tgaacatgac gcccggcacg cccatgtcgt
     4081 tgaacacgac gtggtcgctc gacccgtagt acggctcgac cacgtagcgc caggcgtcgc
     4141 gcgtgccgta ggggtccagg atcgggtagc tgtacttgta tccgccgccc cgctcgtgga
     4201 tgcgctcgat gttcgtcgtg cccacccatt cgaagaactc ctgcgcgacg tccccgagga
     4261 agtggatgcg cgagtacggc atgcggtaca ggcgcgtggc gttgttgttg agggtcacgt
     4321 tctggccggc catgtcgatg ttcacgttgg ccacgacgcg cgagaactcg tcggcccggt
     4381 cctcgatgaa ggcgtacgta ccggtgaact cgtccatcca caggaagcgg atgcttcggg
     4441 cggggcgccg caccgtcccc cgggccatcg cttcgaccag cgcccggccg gtctccagga
     4501 tgagcacgga gcccgagccg tcgtccgccg cgccctgctt ctggatcccc tcgaagaggt
     4561 gcgccgtgta ccagacccac tcgtcggacg agccgtcgcc cggtatgacg gccgtcacca
     4621 cctcgttgtc ggcgcggcgc cactcggcct cgaccaccgc gcggaccgtg acttcgtcat
     4681 tccggatctg gagctcgcgc aggtcgtcca cgatgatggc cgggaggttg aaggcgaagc
     4741 cggcctcggg ctcaccgatc gagctccact ggatggtctc gggcatggtg tacggccgct
     4801 gggccgccat gttgaccacc ccgagggcgc cacgctccgt cgccgcggta aacgccgagc
     4861 gggtggaggc gcgggtaagc acgatcgccc ccgtcacgtc ccgtcccgcg taggcttccg
     4921 gatcggaggc gttctcgatc cagaccagcg gaccctcgta ggtccctgtc acgctgcccg
     4981 gggcgagagc ggccgagatg tcccggaagt cgatcacctt gcgcaggacg ggcgacgtga
     5041 cccatagctc gcctaccgag ccgtcccatt ccggtcctcc ttcgtacggg atgacctcga
     5101 cgtcggagag tccgtactcg cgggccttct ccgcgacgaa ggacgtctcg cgcagcgggc
     5161 cggcgtactc
//
LOCUS       DBNHQZ010000395        11290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2961871, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000395 DBNHQZ010000000
VERSION     DBNHQZ010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2961871"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2447
                     /locus_tag="AC16YU_10770"
     CDS             <1..2447
                     /locus_tag="AC16YU_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065751.1"
                     /translation="RIEELLDGALDREPATRNSWLDEACGDDLELRRSVGALLDAGEE
                     AGTFLEADGPRPVANFREAVLRVREQRQAEKEFVGRQIGPYRVIRRLGKGGMGQVFLA
                     VRDDQSFTRHVALKLIRQGLDTEEILHRFRTEQRILGSLSHPNIARLLDAGAEEGLSY
                     LVMEYIEGIPITKYCEANGLSVRERLELFRVVCGAVHYAHQNLVVHRDLKPGNILVSS
                     DGVPRLLDFGIAKVLNPNLPGVAMPITRPEVRVMTPEYASPEQMRGMSVTTASDVYQL
                     GVLLYELLTGSRPYQTSGLTRDEIERLVCDTEPERPSTAVMAAAPETLDSGAVPEVAP
                     AASRGGRIGRLRRMLEGDLDRIVLMALRKEPARRYQSVDQLQEDVRRYLEGLPVRAQS
                     DTWAYRTAKFVRRHRVSVAVTATAAVLLVVLSVIALRFAVITRAQAERVQVAAARTEQ
                     VRQFLINLFEVADPENARGRQVTARELLEEGAARVERELAGQPDVQADMLSVIGTVYR
                     QLGLYDEAQPFLERSLTMRRSLHGPVHPDVAASLFELGELRVWQGHLGNAEDLHREAL
                     DIRRTTLADRDPLVAASLNGLGVVVAERGRYDEAEGLFREALELREQALGESDAAVAE
                     TIANLAWVLHDDGHLVEAEDQYRRAIAIQRRVLGEDHPDVATTTNNLAALLFDTGNYA
                     AAEPLFREVLDLRTRLYGASHPLVTNAKSWLGRVLQQQGKTDEAEERFREALADHRSQ
                     LGDRHMYVGRDLHILGDLLAATGRHAEAESSLIEALSIYRDNLDSPDHRLIQEARATL
                     SQVYRDWGRPDRSAAY"
     gene            complement(2521..5151)
                     /gene="clpB"
                     /locus_tag="AC16YU_10775"
     CDS             complement(2521..5151)
                     /gene="clpB"
                     /locus_tag="AC16YU_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR6065752.1"
                     /translation="MNLQKFTVKAQEAIQRATGIAASRNHQGLEPPHLLLAFLEEEGG
                     VVEALLHRLGASPDVLRNRATEALDKLPVVTGSSVSGQYVGQDAKKVFDRALAEAEQL
                     KDDFVASEHVLVALAASKDPVGDALRDQGADRAAILGVLRDVRGSQTATDEHAESRYQ
                     ALERYTRDLNALARRGKIDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAE
                     GLAIRIVQGDVPESLRNRRILALDMGALIAGAKYRGEFEDRLKAVVKEVTEAEGQVVL
                     FIDEIHTLVGAGASEGAMDAANILKPALARGELRAIGATTLDEYRKYLEKDKALERRF
                     QTVTVDEPSTGDTVSILRGIKERYEVHHGVRITDGALVAAAELSQRYITDRFLPDKAI
                     DLIDEAASRLRIEIDSMPEELDQLERQIRQLEIEREAVRREKDEAKESRIASQLAELD
                     ERRTALRARWDNEKELIQDLRGAKEEIEELRLEAENMEREGRYGEVAEIRYGRIPELE
                     HHIEETKQKLSDVQKNGALLKEEIDSEDVAEIVSRWTGIPVSRMLESERSKLLRMEDE
                     LARRVVGQAEAVTAVSNAVRRGRAGLQEGSRPIGSFIFLGTTGVGKTELAKALAEFLF
                     NDENALIRIDMSEYQERHTVSRLVGAPPGYVGYEEGGQLTERVRRRPYAVVLLDEIEK
                     AHPEVFNILLQLLDDGRLTDNKGRTADFRNTIVIMTSNLGSELIRERIDAAGERMETA
                     AEDRLRDDLMALLRQRLRPEFLNRIDEIVLFHPLGRAEIASIVDIQFAHVRELARRSA
                     GIELELTDSARAWLADRGFDPVFGARPLKRVLQQEITNKLAEEILSGWIQAGETIRID
                     LADDGAGLTFETTSVGTAAG"
     gene            complement(5296..5931)
                     /locus_tag="AC16YU_10780"
     CDS             complement(5296..5931)
                     /locus_tag="AC16YU_10780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6065753.1"
                     /translation="MLTDSDAAHAHWQRVYASGDPARASWHQAVPRLSLDLIERTDVG
                     LDAAVIDVGGGSSLLVDHLLDRGYLAVSVLDLSSRAIAHARDRLEARAELVTWLCEDV
                     TAMIPERRYALWHDRALFHFLGDPVDRALYAAVLYEAVEPGGWVILATHAPDGPTSCS
                     GLPTCRYDGPALAAELGQGFLLVEEHRETHRTPSGGEQRFAWSVFRRSDDF"
     gene            complement(5932..6321)
                     /locus_tag="AC16YU_10785"
     CDS             complement(5932..6321)
                     /locus_tag="AC16YU_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR6065754.1"
                     /translation="MADYYFARTVDGDLDAVVERVTEALKTEGFGVLTTIDVQATLKN
                     KLDVDFRPYRILGACNPQFAHRALQAEDKIGTMLPCNVVVQDAGGGRTEVAAVDPIAS
                     MAAVGNPALEGIAGEVRERLQRVVRAI"
     gene            complement(6330..7409)
                     /locus_tag="AC16YU_10790"
     CDS             complement(6330..7409)
                     /locus_tag="AC16YU_10790"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR6065755.1"
                     /translation="MGYLPDWVPGLHPLLVHFPIALLFAALLTDLVSLFARSREGALR
                     VANLLYLFGAIAVVAVFFSGREAADQVFLGGEAQSLLTEHSELGEWTLWFFAGYVIVR
                     LPLSVSAFGKSLAMRLVLFVAGLAGVGLMTWTAHHGAELVFRHGAGVQAVEDIPVERP
                     AIRLDSAAADAGGIVSGGKGSWSWTPVRAAAWQESVTWVEGTPGSFGSRLVDGAERGD
                     VLEIELSSETLFFTFPEPLGGIQVDFEADLSEFDGTLAVAHEVTGPDDFRFASFGNGT
                     VRLGRAESGDLLVSETAGADLAGWRAIRVVVDAGHARTYVETVMAAHGHFTPGPAAPV
                     GLRLNGTGRVRLAFMSIVPVSQASE"
     gene            complement(7462..10677)
                     /locus_tag="AC16YU_10795"
     CDS             complement(7462..10677)
                     /locus_tag="AC16YU_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6065756.1"
                     /translation="MLKRIITWSAHNRLLVLLGTGILATLGAWATVNTPVDAIPDLSD
                     VQVIIRTEWPGQSPQIVEEQITYPLATSMLAVPYATTVRGYSMFGTSFVYVIFDDGTD
                     MYWARSRVLEYLNLVSADLPEGVQPALGPDATGVGWVFEYSLVDTTGNLDLSQLRSLQ
                     DFFLKYELQAIDGVAEIATVGGFQRQYQVIVDPQKLAAYGIALADVTDALRRSNQDVG
                     GRLLELGEREFMVRGRGYLRGVDDIRGVPVKRTVGTAATIGDIATVQIGPEIRRGIAE
                     KNGEGEVVGGIVVMRYGENARDVIRNVKARIAELEPGLPPGVRIVPEYDRSMLIDRAV
                     GTLRTKIWQEMAVVLLIVIVFLMHVRSAFVAVVTVPVGILLAMLVMGVLGINANIMSL
                     GGIAIAIGVMVDASLVMVENAHKHLERLGENGPRPPTDAERFGAVLDAAREVGPSLFF
                     SLLIVTVSFLPVFALGQVEGRLFRPLALTKTFAMAAASLLAITLVPALMVTLIRGRIR
                     SEARNPVSRFFIWAYRPAIRFTLRNPWLVILTSFATVLLTLLPMQRMVFGHTWVPFPQ
                     IGSEFMPPLDEGDLLYMPTTLPGISPQKAKELLQQTNRIIKSFPEVKSVFGKIGRAET
                     ATDPAPLDMIETVVVLKPRSEWREGMTHDRLIDELNSSVRIPGLTNAWTMPIRARTDM
                     LATGIRTPVGVKLAGTDLSVLADLAAGVEAAVRDIPGTRSVYAERVTGGSYVDVVVDR
                     PAAARYGLTTGDVLDVVASSIGGRNVTTTVEGLERYPVNVRYPRALRESLPALRNVLV
                     PTPAGGHIPLGQVADLVLVEGPPMIKSENARPNAWVFIDLVEGTDLGAYVARARQQVD
                     GAVTLPAGYSLRWSGQYEYIERANKRLQVLVPLTLAIILLLLVMHFRNLAEATILMIT
                     LPFAVVGALWLLLLLGFKWSLAVGVGLIAVAGLAAETGVVMQVYLDEAVRRFSAEGRL
                     VTRDDLNRALETGAVDRVRPKLMTVLTTIIGLLPVMIGTGTGSEIMQRIAAPMVGGLV
                     SSTILTLIVIPAMYVVYYRFRLSRGTTGADAVTATSGGGAT"
     gene            complement(10681..>11290)
                     /locus_tag="AC16YU_10800"
     CDS             complement(10681..>11290)
                     /locus_tag="AC16YU_10800"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6065757.1"
                     /translation="MEKTVVEGQQVAAGEPLMKIFDTSEIWLVADIYEQDLPWVKVGS
                     RALIQVPYFPDRRLEGRVEHLYYMLNPETRSAEARITLRRVDNVLRPGMYAVVEFEGA
                     PTAPSPVVPEEAIVWTGDQSVIILSVGSGRFRPIAVRTGVQAEGYIQVLEGLSGGEEV
                     VTSAQFLIDSEARLKSAIGAMVGTHDHAGTAGSSSDESGIGG"
BASE COUNT         1710 a         3940 c         3921 g         1719 t
ORIGIN      
        1 agcgcatcga ggagctgctg gacggggccc tggaccgtga gcccgccacg cggaattcct
       61 ggctggacga ggcctgcgga gacgacctgg agctccgccg cagcgtaggg gcgctgctcg
      121 acgccggcga ggaggcgggc acgttcctgg aagcggacgg ccctcgacct gtcgcgaact
      181 tccgggaggc cgtgctccgg gtgcgcgagc agcggcaggc ggaaaaggaa ttcgtgggcc
      241 gccagatcgg cccgtaccgc gtgatccggc gcctgggcaa gggcggcatg gggcaggtgt
      301 tcctcgccgt ccgtgacgac cagtcgttca cgcgccacgt cgcgctcaag ctcatccggc
      361 aggggctcga caccgaggag atcctgcacc gcttccgcac cgagcagcgc atcctgggca
      421 gcctgtcgca cccgaacatc gcacgcctgc tggatgcggg ggcggaggag gggttgtcgt
      481 acctcgtgat ggagtacatc gagggcattc ccatcacgaa gtactgcgag gccaacggcc
      541 tctcggtccg agagcggctc gagctattcc gcgtggtgtg cggcgccgtg cactacgcgc
      601 accagaacct cgtggtgcac cgggacctga agccgggcaa catcctggtg tcgtccgacg
      661 gcgttccgag gctcctggac ttcggcatcg cgaaggtgct gaacccgaat ctccccggcg
      721 tcgcgatgcc catcacgcgc ccggaggtcc gggtgatgac gcccgaatac gcctctcccg
      781 agcagatgcg cgggatgtcg gtgaccaccg cgtccgacgt ttaccagctg ggcgtgctgc
      841 tctacgagct ccttaccggg tcgaggccgt accagacctc cggcctcact cgcgacgaga
      901 tcgagcgcct ggtctgcgac accgagccgg agcgcccgtc gacggccgtg atggcggcgg
      961 cgccggagac cctggactcc ggcgccgttc cggaggtcgc ccccgccgcg tcgcgcggcg
     1021 gaaggatcgg ccgtctgcgc cgcatgctcg agggcgacct ggaccgcatc gtgctcatgg
     1081 cgctgcgcaa ggagccagcg cggcgctacc agtccgtgga ccagctccag gaggacgtgc
     1141 ggcgctacct cgagggcctc cccgtccggg cgcagtcgga cacctgggcc taccgcacgg
     1201 ccaagttcgt tcggcggcac cgggtcagcg tcgcggtcac ggccacggcc gcggtgctgc
     1261 tcgtcgtcct ttccgtgatc gcgcttcgct tcgccgtcat cacgagggcg caggccgagc
     1321 gcgtgcaggt cgccgcggcg cggacggaac aggtccggca gttcctgatc aacctgttcg
     1381 aggtagccga ccccgagaac gcgcgcggcc ggcaggtcac ggcccgggag ctcctggagg
     1441 agggtgccgc gcgggtggag cgcgagctgg ccggccagcc cgacgtgcag gccgacatgc
     1501 tgtcggtgat cggcaccgta taccggcagc tcggactcta cgacgaggcg cagcccttcc
     1561 tggagcgttc gctcaccatg cggcgctcgc tccatggccc cgtgcatccc gatgtcgcgg
     1621 cgagcctttt cgagctgggc gagctccgcg tgtggcaggg ccacctggga aacgccgagg
     1681 acctgcaccg ggaggcgctc gacatccggc ggacgacgct tgcggaccgg gatccgctcg
     1741 tcgcggcgag cctcaacggc ctgggcgtgg tggtcgccga gcgcggccgc tacgacgagg
     1801 cggaagggct cttccgcgag gcgctcgagc tacgggagca ggcactcggc gagtcggacg
     1861 cggcggtcgc cgagacgatc gccaacctcg cgtgggtcct gcatgacgac gggcacctgg
     1921 tcgaggccga ggaccagtac cgccgcgcca tcgcgatcca gcggagggtg ctcggcgagg
     1981 accacccgga cgtggccacg acgaccaaca acctcgcggc ccttctcttc gacaccggga
     2041 attacgcggc tgcggagccg ctcttccgcg aagtgctgga ccttcggacc cggctgtacg
     2101 gcgcgagcca cccgctagtc acgaacgcga agagctggct cgggcgcgtc ctgcagcagc
     2161 agggcaagac ggacgaggcc gaggagcgtt tccgggaggc cctggccgac caccggagcc
     2221 agctcggcga ccggcacatg tacgtgggac gcgacctgca catcctgggc gacctgctcg
     2281 ccgcgaccgg ccgccacgcc gaggccgaat cgagcctgat cgaggcgctg tcgatctacc
     2341 gcgacaacct ggacagcccg gaccaccggc tcatccagga agcccgcgcc accctctcgc
     2401 aggtgtacag ggactgggga cgcccggacc gctcggcggc gtactgagaa gggctgccag
     2461 gccccgcccc gcgacgcgat cagaccgccc gggcacctcc tttcgcgacg ggtgtgatcg
     2521 tcagccggcg gccgtgccga cgctggtggt ctcgaacgtg aggcccgccc cgtcgtccgc
     2581 caggtcgatg cggatggtct cgcccgcctg gatccagccg gacaggattt cctcggccag
     2641 cttgttcgtg atctcctgct gcaggacgcg cttcagcggc cgggcgccga acaccgggtc
     2701 gaagccgcgg tccgccagcc aggcccgcgc cgagtccgtg agttcgagct cgatgccggc
     2761 gctgcggcgg gcgagttccc gcacgtgggc gaactggatg tcgacgatcg atgcgatctc
     2821 ggcgcgtccg agcggatgga acaggacgat ctcgtcgatg cggttcagaa attccgggcg
     2881 cagccgctgg cgcaggagcg ccatgaggtc gtcgcgaagc cgatcctcgg ccgcggtctc
     2941 catgcgctcg cccgccgcat cgatccgctc ccggatcagc tcggaaccga ggttgctcgt
     3001 catgatgacg atggtgttcc ggaagtccgc ggtgcggccc ttgttgtcgg tcagccgccc
     3061 gtcgtcgagc agctgcagga ggatgttgaa cacctcgggg tgagccttct cgatctcgtc
     3121 gagcagcacg acggcgtagg ggcgccggcg cacccgctcg gtgagctggc cgccctcctc
     3181 gtatcccacg tatccgggag gggcgcccac gagccggctc accgtgtgcc gctcctggta
     3241 ctcgctcatg tcgatgcgga tgagcgcgtt ctcgtcgttg aacaggaatt cggcgagggc
     3301 cttcgccagc tcggtcttgc cgacgccggt cgtgcccagg aagatgaacg agccgatggg
     3361 ccggctgccc tcctggaggc cggcacggcc ccggcgcacg gcgttggaga cggcggtcac
     3421 ggcctcggcc tgcccgacca cgcgcctggc gagctcgtcc tccatgcgca ggagcttcga
     3481 gcgctcgctc tcgagcatgc gcgataccgg gatgccggtc cagcgcgaca cgatctcggc
     3541 cacgtcctcc gagtcgatct cctccttgag cagggcgccg ttcttctgga cgtccgagag
     3601 cttctgcttc gtctcctcga tgtggtgctc cagttccggg atcctgccgt agcggatctc
     3661 cgccacctcg ccgtagcgac cttcgcgctc catgttctcc gcctccaggc gcaactcctc
     3721 gatctcctcc ttggccccgc gcaggtcctg gatgagctcc ttctcgttgt cccagcgggc
     3781 gcgaagggcc gtgcggcgct cgtcgagctc ggccagctgc gatgcgatgc gggattcctt
     3841 cgcctcgtcc ttctcgcggc ggaccgcctc gcgctcgatc tcgagctgac ggatctggcg
     3901 ctcgagctgg tccagctcct cgggcatgga gtcaatctcg atgcgcaggc gggaggcggc
     3961 ctcgtcgatg aggtcgatcg ccttgtcggg caggaagcgg tccgtgatgt agcgctggct
     4021 cagctcggcg gccgcgacca gggcgccgtc cgtgatccgt acgccgtgat gcacctcgta
     4081 ccgctccttg atgccgcgca ggatcgacac ggtgtcgccc gtggacggct cgtcgaccgt
     4141 cacggtctgg aagcggcgct cgagcgcctt gtccttctcc aggtacttgc ggtactcgtc
     4201 gagagtcgtc gccccgatcg cccggagctc gccacgcgcg agggcgggtt tcagaatgtt
     4261 ggccgcgtcc atggcaccct cggacgcgcc cgcgccgacg agcgtgtgga tctcgtcgat
     4321 gaagaggacc acctggccct ccgcctcggt cacctccttc acgacggcct tgagccggtc
     4381 ctcgaactcg ccgcggtact tggcccccgc gatgagcgcg cccatgtcga gcgccaggat
     4441 acgccggttg cggaggctct cgggcacgtc cccctggacg atccggatgg cgagcccctc
     4501 ggcgatcgcg gtcttaccca cgcccggctc gccgatgagc accgggttgt tcttggtgcg
     4561 gcgcgacagg atctgcagga cgcgccggat ctcgtcgtcc cggccgatga ccggatcgat
     4621 cttgcctctc cgcgcgaggg cgttcaggtc ccgcgtgtac cgctccaggg cctggtagcg
     4681 gctctcggcg tgctcgtccg tcgcggtctg cgatccgcgg acgtcgcgca gcacgcccag
     4741 gatggccgcg cggtccgcac cctggtcccg gagcgcgtcg cccacggggt ccttcgacgc
     4801 cgcgagcgcg acgagcacgt gctcgctcgc cacgaagtcg tccttgagct gctcggcctc
     4861 ggccagtgcc cggtcgaaga ccttcttggc gtcctggccc acgtactgcc cggagacgct
     4921 cgaccccgtg acgacgggga gcttgtccag cgcctccgtg gcccggttac ggagcacgtc
     4981 gggactcgcc ccgagccgat ggagcagggc ttcgacaacc ccgccctcct cctccaggaa
     5041 ggcgaggagc aggtggggcg gctccaggcc ctggtggttt cgcgacgcgg cgattccggt
     5101 ggccctctgg atggcttcct gggccttgac ggtgaatttc tgcaggttca tgggctgggc
     5161 tcgtacgttt ccccggatcg ggaccgccga ccgacacgat ggcaggcggc ttctccccgc
     5221 aaggccttcc gggacccgtc caagtccgtg ccaaagggcg ccgtcctgac gggctggcag
     5281 tcccgtcgcg gcggatcaga aatcgtccga gcggcggaag accgaccagg cgaaccgctg
     5341 ctccccgccg gacggggtcc ggtgcgtctc ccggtgctcc tcgacaagga ggaatccctg
     5401 cccgagttcg gccgcgagcg ccggcccgtc gtagcggcac gtgggcaggc cgctgcacga
     5461 ggtcgggccg tccggagcgt gggtcgcgag gatcacccac ccgcccggtt cgacggcctc
     5521 gtacaggacg gcggcgtaca gggcacggtc caccgggtct cccaggaagt ggaacagggc
     5581 gcggtcgtgc cagagggcat accggcgctc cgggatcatc gccgtcacgt cctcgcagag
     5641 ccaggtcacg agctccgccc gggcctcgag gcggtcccgc gcatgggcga tggcccgcga
     5701 cgagaggtcc agcacgctca cggccaggta cccgcggtcg agcaggtggt ccacgaggag
     5761 cgaggagccg ccgccgacgt cgatgacggc cgcgtccagg ccgacgtccg tccgctcgat
     5821 gaggtccagg gagagtcggg gcacggcctg gtgccagctc gcccgcgcgg gatcacccga
     5881 ggcgtagacg cgctgccagt gcgcgtgggc cgcgtccgaa tcggtcagca ttcagatcgc
     5941 ccgtacgacg cgctggagac gttcccgtac ctcgcccgcg ataccctcca gggcgggatt
     6001 cccgaccgcg gccatcgagg cgatcggatc cacggccgcc acctcggtcc ggcccccgcc
     6061 ggcgtcctgc accacgacgt tgcagggcag catggtgccg atcttgtcct cggcctgcag
     6121 ggccctgtgg gcgaactggg gattgcacgc cccgaggatg cgatacggtc tgaaatccac
     6181 gtcgagcttg ttcttgaggg tggcctgcac gtcgatcgtg gtcaaaacgc cgaacccttc
     6241 cgtcttcaga gcctcggtca ctcgttcgac gaccgcgtcc aggtcgccgt ccacggtccg
     6301 ggcgaaatag tagtcagcca tggtccgatt cactccgatg cttggcttac cggtacgatg
     6361 gacatgaacg ccaggcggac gcgtccggtc ccgttcagac gcagcccgac gggcgccgcc
     6421 ggaccgggag tgaagtggcc gtgagcggcc atgacggttt ccacgtaggt gcgggcatgc
     6481 ccggcgtcga ccaccactcg gatggcccgc cacccggcga ggtcggctcc ggccgtctcc
     6541 gacaccagga ggtctccgga ctccgcgcgg ccgagccgca ccgtcccgtt gccgaaggag
     6601 gcgaagcgga agtcgtcggg accggtcacc tcgtgggcga ccgcgagcgt gccgtcgaac
     6661 tcggacaggt ccgcctcgaa atcgacctgg atcccgccca gcggctccgg gaaggtgaag
     6721 aagagggtct cgctcgagag ctcgatctcg agcacgtcgc cgcgctcggc gccgtccacc
     6781 aggcgggacc cgaaggagcc cggcgtcccc tcgacccacg tcaccgattc ctgccaggcc
     6841 gccgcccgca ccggcgtcca cgaccacgag cccttgcccc cgctcacgat gcccccggcg
     6901 tcggcggcag cgctgtccag gcggatggcg gggcgctcga ccgggatgtc ttcgaccgcc
     6961 tgcacgccgg cgccgtgccg gaagacgagc tcggccccgt gatgggccgt ccaggtcatc
     7021 agcccgacgc ccgcgaggcc ggcgacgaac aggaccaggc gcatcgccag gctcttgccg
     7081 aaggcgctga ccgacagcgg aagccggacg atgacgtagc ccgcgaagaa ccacagggtc
     7141 cactcgccga gctcggaatg ctcggtgagc agcgactgcg cctcccctcc gagaaagacc
     7201 tggtcggcgg cctcgcggcc gctgaagaag acggccacga cggcgatcgc gccgaacagg
     7261 tacagcaggt tggccacccg cagcgccccc tcccgggacc gggcgaaaag cgagacgagg
     7321 tcggtcagca gtgccgcgaa gagcagggcg atcgggaagt gtacgaggag cgggtgcagg
     7381 cccggcaccc agtccggcag gtatcccatg gcaacgcagg cggttgttgt gcccccgtat
     7441 aacccgcttc ggtctgcaag gttacgttgc gccgccgccg gaggtcgccg tgacggcgtc
     7501 tgcgccggtt gtgccccggg acagccggaa gcggtaatag accacgtaca tcgcaggaat
     7561 gacgatcagc gtgaggatgg tcgagctcac gaggccaccc accatcgggg cggcgatgcg
     7621 ctgcatgatc tcggaccccg ttcctgtgcc gatcatcacc gggagcaggc cgatgatggt
     7681 ggtcaggacc gtcatgagct tcggacggac gcggtcgacc gcgcccgtct cgagcgcccg
     7741 gttcaggtcg tcgcgcgtca cgagccgccc ctcggccgag aagcgccgga ccgcctcgtc
     7801 caggtacacc tgcatcacca cgccggtctc ggccgcgagc cccgcgacgg cgatcaggcc
     7861 gaccccgacg gcgaggctcc acttgaaccc gagcagaagg agaagccaca gcgctccgac
     7921 caccgcgaac ggcagcgtga tcatcaggat cgtggcctcg gccaggttcc ggaagtgcat
     7981 gaccagaagg agcaggatga tggccaacgt cagcgggacc aggacctgca ggcgcttgtt
     8041 cgcgcgctcg atgtactcgt actggccgct ccagcgcagg ctgtaacccg ccggaagcgt
     8101 gacggccccg tcgacctgct gtctggcccg ggcgacgtac gcgcccaggt cggtgccctc
     8161 caccaggtcg atgaacaccc aggcgttcgg gcgcgcgttt tcgctcttga tcatgggcgg
     8221 accctccacc aggaccaggt ccgcgacctg cccgagggga atgtggccgc cggccggcgt
     8281 cggaacgagc acgttgcgca gcgccggcag gctctcgcgg agggccctcg gatagcgaac
     8341 gttcacgggg tatcgctcca ggccctccac ggtggtcgtg acattgcggc cgccgatcga
     8401 ggaggccacg acgtccagga cgtcgcccgt ggtcagtccg taccgggccg cggcgggccg
     8461 gtccacgacc acgtccacgt aggatccgcc ggtcacgcgc tccgcgtaca cggaccgcgt
     8521 cccggggatg tcgcgcacgg cggcctccac ccctgcggcg aggtcggcga gcacggacag
     8581 gtcggtcccg gcgagcttga cgccgaccgg cgtccggatg cccgtcgcca gcatatcggt
     8641 gcgggcgcgg atgggcatcg tccacgcgtt cgtgagtccg ggaatccgga cggaggaatt
     8701 cagctcgtcg atgagccggt cgtgggtcat gccttcgcgc cactcgctcc gtggcttgag
     8761 caccacgacc gtctcgatca tgtcgagcgg tgcaggatcg gtggccgtct cggcccggcc
     8821 gatcttcccg aacacgctct tgacctcggg gaagctcttg atgatgcggt tcgtctgctg
     8881 caggagctcc ttggccttct ggggcgagat gcccgggagg gtagtgggca tgtacaggag
     8941 gtcgccctcg tcgagcggcg gcatgaactc cgatccgatc tgcgggaagg gcacccaggt
     9001 gtgcccgaac accatcctct gcatgggcag gagggtaagc aggacggtgg cgaagctcgt
     9061 caggatgacc agccacgggt tccggagcgt gaagcggatc gcgggacgat aggcccagat
     9121 gaagaaccgc gagaccgggt tgcgcgcctc gctccgtatg cgtccccgga tcagggtgac
     9181 catgagggcc ggcacgagcg tgatggcgag aagcgatgcg gccgccatgg cgaaggtctt
     9241 tgtgagggcc agcggccgga acaggcggcc ctccacctgg ccaagcgcga agacgggaag
     9301 gaaggagacc gtcacgatga gaaggctgaa gaagaggctg ggcccgacct cccgggccgc
     9361 gtcgagcacg gccccgaacc gctcggcgtc cgtgggcggt cgcgggccgt tctcgcccag
     9421 gcgctccagg tgcttgtggg cgttctcgac catgaccagc gacgcgtcga ccatgacgcc
     9481 gatggcgatc gcgatcccgc ccaggctcat gatgttcgcg ttgatgccga gcactcccat
     9541 caccagcatc gcgaggagga tgccgaccgg caccgtcacg acggccacga aggcgctccg
     9601 cacgtgcatc aggaaaacga tgacgatgag cagcacgacc gccatttcct gccagatctt
     9661 ggtccggaga gtgccgacgg cccggtcgat gagcatcgag cggtcgtatt ccggcacgat
     9721 gcgcaccccg ggcggcagtc ccggctcgag ctctgcgatc cgggccttga cgttccggat
     9781 cacgtcccgc gcgttctcgc cgtagcgcat caccacgatg ccgcccacga cctctccttc
     9841 gccgttcttc tccgcgatgc ctcgccggat ctcgggcccg atctgcacgg tcgcgatgtc
     9901 gccgatcgtg gccgccgtgc cgacggtccg cttcaccggg acgccccgga tgtcgtccac
     9961 cccgcggaga tagccgcgcc cccgcaccat gaactcgcgc tcgccgagct cgagcaggcg
    10021 cccgccgacg tcctggttgg agcggcggag ggcgtcggtg acgtcagcca gggcgatgcc
    10081 gtaggccgcc agcttctgcg gatcgacgat gacctggtac tggcgttgga atccgcccac
    10141 cgtggcgatc tcggcgacgc cgtcgatggc ctgcagctcg tacttcagga agaagtcctg
    10201 cagggagcgg agctgggaca ggtccaggtt gccggtcgtg tccaccagcg agtactcgaa
    10261 cacccatccg acgccggtcg cgtcggggcc gagggccggc tggacgccct cggggaggtc
    10321 tgcgctgacc aggttcaggt actccagcac ccgggacctg gcccagtaca tgtcggttcc
    10381 gtcgtcgaag atcacgtaga cgaacgacgt gccgaacatc gagtacccgc ggaccgtggt
    10441 ggcgtacggc acggccagca tggaagtcgc gagcgggtac gtgatctgct cctcgacgat
    10501 ctgggggctc tgaccgggcc actccgtccg gatgatgacc tgcacgtccg agaggtccgg
    10561 gatggcgtcg accggcgtgt tgaccgtggc ccaggcgccc agcgtggcga ggatgcccgt
    10621 gccgaggagg accaggaggc ggttgtgcgc gctccaagtg atgatgcgtt tcagcatggc
    10681 tcagcctccg atgccggatt cgtcggacga ggagcccgcc gtgccggcgt ggtcatgcgt
    10741 gcccaccatg gcgccgatcg cgcttttgag gcgggcctcg gagtcgatca ggaactgggc
    10801 gctcgtcacg acctcctcgc cgcccgagag gccctcgagc acctggatgt agccttccgc
    10861 ctggaccccc gtccggaccg cgatcggacg gaaccggccc gagccgaccg acaggatgat
    10921 tacggactgg tcaccggtcc acacgatcgc ctcctccggg accacgggcg acggcgcggt
    10981 cggcgctccc tcgaactcca cgaccgcgta catgccgggc cggagcacgt tgtccacccg
    11041 ccgcagggtg atgcgtgcct ccgccgagcg ggtctccggg ttgagcatgt agtagaggtg
    11101 ctcgacccgg ccctcgagcc gccggtcggg gaagtacgga acctggatca gcgccctgga
    11161 tcccaccttc acccacggca ggtcctgctc gtagatgtcg gcgaccagcc agatctcgct
    11221 cgtgtcgaag atcttcatga ggggctcgcc cgctgcgacc tgctggccct ccactacggt
    11281 cttctccatg
//
LOCUS       DBNHQZ010000396         3813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3505223, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000396 DBNHQZ010000000
VERSION     DBNHQZ010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3505223"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16YU_10805"
     CDS             complement(<1..485)
                     /locus_tag="AC16YU_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065758.1"
                     /translation="MIGWTLRMAWRDSRGSRRRLVLFVGSMVLGVAALVAINSFGDNL
                     RLAVDEQARTLLGADLSLESGQPFPDSIEAVIAGLGGEQARRTSFASMALFPRTGGTR
                     LATVRAIEGPFPWYGSIGTDPPEAASSYRARGAALVDKTLMQQFGLSVGDSVRIGSRS
                     YA"
     gene            complement(482..1192)
                     /locus_tag="AC16YU_10810"
     CDS             complement(482..1192)
                     /locus_tag="AC16YU_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065759.1"
                     /translation="MSAVLEVRSLGKTYASGGRPLSVLRDVSFTLDERDTCAVVGPSG
                     SGKTTLLGLCAGLDSPSTGDVTLCGEVLGTLDEDARALVRNRHVGFVFQSFRLIPSLT
                     ALENVMVPAELRGDRTSRDRAAELLRTVGLGDRLGHYPAQLSGGEQQRVALARAFVNR
                     PSILFADEPTGNLDTETGDRVVELLFDLNRTAGTTLVVVTHDLELAARTGRVIRLRNG
                     GVESDSGSPAEVAAEETR"
     gene            1253..1978
                     /locus_tag="AC16YU_10815"
     CDS             1253..1978
                     /locus_tag="AC16YU_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR6065760.1"
                     /translation="MTWFARLYPLLTICVLAAAGCGGEESGNGAGAPGPTTAATDAST
                     PRSEAASATVMFFGDSITAGYGIGAEDAFPALVQARADSAGLPVRVVNAGLSGETSAG
                     GLRRIGWVLREPVDVFVLELGGNDALRGTDLAATRRNLQAIVDSVRSRNPATRIVIAG
                     MMIPPNFGAAYAGAFRDLFPDLASANDAVLIPFLLDGVARVDGMMQPDGIHPTRSGHR
                     VMAETTWRYLEPELRELFPAASP"
     gene            complement(2052..3515)
                     /locus_tag="AC16YU_10820"
     CDS             complement(2052..3515)
                     /locus_tag="AC16YU_10820"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR6065761.1"
                     /translation="MEAIIKIAYLVSAILFIFGMKRLQSPATARGGNQLAAVGMLVAV
                     AATLISKSIVTPAEMIAGLVVGGAVGAFLAKRVEMTGMPELVAAFNGFGGAASAVVAG
                     AEIAKLIQNTGTASGEEFYIYTAVTISLSILIGMVTFTGSFVAFGKLSGKMSGNPISF
                     PGMRFLTLLLGVVSIAALVWMAIGAGAYPFEAYTTQAVSIEFTEPMIYGALAIAGAAF
                     ILGILLVIPIGGADMPVVVSLLNSYSGMAAAATGFVLNNTALIVSGALVGASGLILTK
                     IMCDAMNRSLLNVLIGGFGGDSQIAAAGTKSLEGKSITETSPEDTAILLSYAEKVIFV
                     PGYGMAVAQAQHAVRELGEELQKRGVTVKYAVHPVAGRMPGHMNVLLAEANVPYDQLF
                     EMDEINGEFDTCDVSVIIGANDVVNPAARHDTASPIYGMPILNVDHSRTSIVLKRSMR
                     PGYAGIENELFYNSNNRMLFGDAKDSITKVVNEIKAL"
     gene            complement(3562..>3813)
                     /locus_tag="AC16YU_10825"
     CDS             complement(3562..>3813)
                     /locus_tag="AC16YU_10825"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR6065762.1"
                     /translation="FTLASLIGFEVISKVPQTLHTPLMSGSNAISGITIVGALIVAGM
                     VEGAWATWLGFAALIFATINVVGGFLVTDRMLQMFRKRD"
BASE COUNT          621 a         1296 c         1320 g          576 t
ORIGIN      
        1 gcgtaggacc ggctcccgat gcggacgctg tccccgaccg acagcccgaa ctgctgcatg
       61 agggtcttgt cgacgagcgc cgcgccgcgt gcccggtagc tcgacgccgc ctccggcgga
      121 tcggtcccga tcgagccgta ccacgggaac ggcccctcga tggcgcgcac ggtggcgagg
      181 cgcgtgccgc ccgtcctcgg gaacagcgcc atcgacgcga acgaggtgcg gcgcgcctgc
      241 tcgccgccca gtcccgcgat gacggcctcg atggaatcgg ggaacggctg accgctctcg
      301 agcgagaggt cggcgccaag cagcgtacgc gcctgctcgt ccaccgccag gcgcaggttg
      361 tccccgaacg agttgatcgc gacgagggcc gcgacgccca ggaccatcga accgacgaag
      421 agcaccagcc ggcgcctgct cccccggctg tcgcgccacg ccatgcgaag cgtccagccg
      481 atcatcgggt ctcctccgcc gcaacttcgg cgggcgagcc gctgtcggat tcgacacccc
      541 cgttgcgcag gcggatcacc cggcccgtcc gggcggcaag ctccaggtcg tgcgtgacca
      601 cgacgagcgt cgtacccgcc gtccggttca ggtcgaagag cagctccacc acgcggtcgc
      661 cggtctccgt gtccaggttt ccggtcggct cgtccgcgaa caggatactg gggcggttca
      721 cgaaggcccg cgcgagggcg acccgctgct gctcccctcc cgagagctgc gcggggtagt
      781 gccccagcct gtcgccgagc cccacggtcc gcagcagctc ggccgctcgg tccctcgagg
      841 tccggtcccc ccgcagctcc gccggaacca tcacgttttc gagggcggtg agcgagggga
      901 tgaggcggaa ggactggaag acgaagccga cgtggcgatt ccggacgagg gcgcgcgcgt
      961 cctcgtccag ggtcccgagg acctccccgc acagcgtcac gtcaccggtc gaagggctgt
     1021 ccaggcccgc acacaggccg aggagcgtcg tcttcccgga gccggatgga cccacgaccg
     1081 cgcacgtgtc ccgctcgtcg agcgtgaagc tgacgtcgcg cagtacggag agcggccggc
     1141 cgccgctcgc gtacgtcttg cccagggaac ggacctcgag taccgcagac attgcgggga
     1201 aacctcctcg tacaggccgg gtacgcgacg gcactccaac ctattggaat cgatgacgtg
     1261 gttcgcgcgc ctatacccgc tcctgacgat ctgtgttctc gccgcagccg gctgcggagg
     1321 cgaagaatcg ggtaatggcg cgggtgcgcc cgggccgacg accgcggcga ccgatgcatc
     1381 gacgccccgc tcggaggcgg cgtccgcgac cgtgatgttc ttcggcgact cgatcacggc
     1441 cgggtacggg atcggcgcag aggatgcctt tcccgcgctc gtccaggcgc gcgcggattc
     1501 ggcgggcctg cccgtccgcg tggtgaacgc cggcctgagc ggcgaaacct cggccggggg
     1561 ccttcggcgc atcgggtggg tgctccggga gccggtggac gtcttcgtcc tggagctcgg
     1621 cggcaacgat gcgctccgcg ggacggacct ggcggccacg aggcgcaacc tccaggcgat
     1681 cgtcgactcg gtgcgctcgc gcaatcccgc cacgcgcatc gtgatcgcgg gcatgatgat
     1741 tccgcccaac ttcggcgcgg catacgccgg ggcatttcgt gacctctttc ccgacctggc
     1801 ctccgccaac gacgccgtcc tcataccctt cctgctcgac ggggtggcga gggtggacgg
     1861 gatgatgcag ccggacggga tccaccccac ccggtccggc caccgcgtga tggcggaaac
     1921 gacgtggcgt tacctggagc ccgagctccg ggagctcttc ccggccgcga gcccctgacc
     1981 cgaacgggag cgcccgggac ccatgcggac cccgggcgca tctatgccag tcccggtgaa
     2041 ggtggcgccg gtcagagcgc cttgatctcg ttgaccacct tggtgatcga atccttcgcg
     2101 tcgccgaaga gcatgcggtt gttcgagttg tagaacagct cgttctcgat gccggcgtac
     2161 ccggggcgca tggagcgctt caggacgatg gacgtcctcg aatggtcgac gttcaggatc
     2221 ggcatgccgt agatggggct ggccgtgtcg tgccgggcgg ccgggttgac cacgtcgttc
     2281 gccccgatga tcacggacac gtcgcaggtg tcgaactcgc cgttgatctc gtccatctcg
     2341 aagagctggt cgtaggggac gttcgcctcg gcgagcagga cattcatgtg gccgggcatg
     2401 cgccccgcga ccgggtgcac cgcgtacttg acggtgacgc cgcgcttctg cagctcctcg
     2461 cccagctccc gcaccgcgtg ctgcgcctgc gccacggcca tgccgtagcc cggcacgaag
     2521 atcaccttct ccgcgtacga cagcaggatg gcggtgtcct cgggcgaagt ctcggtgatg
     2581 gacttgccct cgagcgactt cgtgccggcg gccgcgatct gggaatcacc accgaaaccg
     2641 ccgatgagca cgttcaggag ggagcggttc atcgcgtcgc acatgatctt cgtaaggatc
     2701 aggcccgacg cgccgaccag ggcgcccgac acgatgagcg cggtgttgtt caggacgaat
     2761 cccgtcgccg ccgcggccat tccggagtag gagttcagga gcgacacgac gaccggcatg
     2821 tcggccccgc cgatggggat gacgagcagg atgccgagga tgaacgcggc cccggcgatg
     2881 gcgagggccc cgtagatcat cggctccgtg aactcgatcg atacggcctg cgtcgtgtac
     2941 gcctcgaacg ggtacgcccc ggcaccgatg gccatccaga caagcgcggc gatcgagacg
     3001 acaccgagca gcagggtcag gaagcgcatc cccgggaagg aaatcgggtt gcccgacatc
     3061 ttcccggaga gcttgccgaa ggccacgaag ctgcccgtga acgtgaccat gccgatcagg
     3121 atcgagaggg agatcgtgac ggccgtgtag atgtagaact cctcgccgct cgcggtcccc
     3181 gtattctgga tgagcttggc gatctcggct ccggccacca cggccgaggc cgcgccgccg
     3241 aaaccgttga acgccgcgac gagctcgggc atgcccgtca tctcgacgcg cttcgcaaga
     3301 aaggcgccga cggcgccgcc gacgacgagg ccggcgatca tctcggcggg cgtgacgatg
     3361 gacttggaga tcagcgtcgc ggcaacggcc acgagcatgc cgaccgctgc gagctggttg
     3421 ccgccacgcg cggtggccgg cgactggagc cgcttcatcc cgaagatgaa gaggatggcg
     3481 gacaccaggt aggcgatttt gatgatggct tccatgtcgg atcgaatggg ttctgtcggg
     3541 cgacggaatc cgcggctgcg gtcagtcgcg cttccggaac atctggagca tgcggtcggt
     3601 cacgagaaag ccgccgacga cgttgatcgt ggcaaagatc agggcggcga acccgagcca
     3661 cgtggcccag gcgccctcga ccatgcccgc aacgatcagg gcgcccacga tggtgatgcc
     3721 gctgatggcg ttcgatcccg acatgagggg agtgtgcagg gtctgtggga ccttgctgat
     3781 gacctcgaac ccgatcaggg atgcgagcgt gaa
//
LOCUS       DBNHQZ010000397         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2557548, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000397 DBNHQZ010000000
VERSION     DBNHQZ010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2557548"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="AC16YU_10830"
     CDS             complement(<1..693)
                     /locus_tag="AC16YU_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065763.1"
                     /translation="MIQVLFLLALWTSFQGGPDDPERASLRAQLVHGPVIERLATAPY
                     GPQESPPAVNRRVEGRRSVPLAFALSVVVPGAGQAYNRQWVKAGVAVAIEAVAVTAWV
                     VRRRDGLAAEDDVEAFAHAGWDPGRYAEWLTDYADYLEVTYGHTVTAPPPDIPAGVDF
                     SSPGSWSDADRLAVDRFFDQVHALERQVYHPETGASFSHVLPYFGEQQYYELIGKYFQ
                     FAPGWSDYPDWID"
     gene            complement(701..1381)
                     /locus_tag="AC16YU_10835"
     CDS             complement(701..1381)
                     /locus_tag="AC16YU_10835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6065764.1"
                     /translation="MDQNLILLAAVLLIAVGVTFLYLLLGRRPGWMPRDAMEWTGSVL
                     SVALIVSAVALIVTAFRVESATSPAMMVAPGGSPPEIVDMALDADAGNFAYRLVEDGS
                     EEDLAGLEGKVVLVNFWATWCAPCLSEIPELNRLAKEYGDRGLVVLSVSDEDPALLSD
                     FDARFGLDTRSVHVQPGAPLPAPFTQAFDVRPTSFVIDRTGRVKRYMLGARSFDIFER
                     FVLPHLEG"
     gene            complement(1415..>1837)
                     /locus_tag="AC16YU_10840"
     CDS             complement(1415..>1837)
                     /locus_tag="AC16YU_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065765.1"
                     /translation="DYVGGLTGARGYPFYALGGNKTLWAQAAYTFPILPDVRRQVLFL
                     YIDKVYARLYGDAAAAWTGPWSGSGGFRKDVGAELRFGLGSFYLLPTAVFVSGTYGLD
                     SFDFRLDADFVTPDGRSSVRYGRSMQWHVGVLFGFDYL"
BASE COUNT          331 a          650 c          621 g          235 t
ORIGIN      
        1 gtcgatccag tccgggtagt cgctccagcc gggcgcgaac tggaagtact tcccgatcag
       61 ctcgtagtac tgctgctcgc cgaaatacgg cagcacgtgc gaaaacgagg cccccgtctc
      121 cggatggtag acctgccgct cgagggcgtg gacctggtcg aagaaccggt ccacggcgag
      181 ccgatcggcg tcgctccacg acccgggcga cgagaagtcg acgcccgcag ggatatcggg
      241 cggcggcgcg gtgacggtgt gtccgtaggt cacctccagg tagtcggcgt agtccgtgag
      301 ccactcggca tagcgccccg gatcccaccc ggcatgggcg aacgcctcga cgtcgtcctc
      361 ggccgcgagc ccgtcgcgcc ggcggacgac ccaggcggtc acggccacgg cctcgatggc
      421 caccgccacg ccggccttga cccactgccg gttgtaggcc tggcccgcgc ctggcaccac
      481 gaccgacagg gcgaatgcca ggggaacgct ccgccgccct tccacccgcc ggttgacggc
      541 gggcggggac tcctgcgggc cgtacggagc ggtcgcgagc cgctcgatca cgggcccatg
      601 gacgagctgc gccctgagcg acgcccgttc cggatcgtcc gggccgccct ggaacgaggt
      661 ccagagcgcc agcaggaaga ggacctggat catgcgcgcg tcacccctcg agatggggca
      721 ggacgaagcg ctcgaagatg tcgaagctcc gggcccccag catgtaccgc ttgacccgcc
      781 cggtccggtc gatcacgaag ctggtcggac gcacgtcgaa cgcctgggtg aacggggccg
      841 gaagcggcgc gccgggctgc acgtgcacgc tgcgcgtgtc caggccgaag cgcgcatcga
      901 aatccgaaag cagcgcgggg tcctcgtccg acaccgagag cacgaccagc ccacggtcgc
      961 cgtactcctt cgccagccgg ttcagctcgg ggatctcgga gaggcacggc gcgcaccacg
     1021 tggcccagaa attgacgagc accaccttgc cctcgagacc cgccaggtcc tcctccgatc
     1081 cgtcctcgac gagccggtac gcaaaattgc cggcatccgc gtccagcgcc atgtcgacga
     1141 tctccggcgg gctgccgccc ggcgcgacca tcatggccgg ggaggtcgcc gattccacgc
     1201 ggaacgcggt cacgatgagc gccacggcgg acacgatgag ggcgaccgag agcacgctcc
     1261 ccgtccactc catcgcgtcg cggggcatcc agccgggccg gcggcccagg agcaggtaca
     1321 ggaacgtcac gccgaccgcg atcaggagca ccgcggcgag aagaatcagg ttctggtcca
     1381 tcgggaatga ggtcaggaag ggccgaagag accctcacag gtagtcgaag ccgaacagta
     1441 cccccacgtg ccactgcatc gagcggccgt accgcacgct ggaccggccg tcgggtgtga
     1501 cgaagtccgc atccagcctg aagtcgaagg agtccagccc gtacgtgccc gatacgaaga
     1561 cggccgtcgg cagcaggtag aacgaaccga gaccgaatcg cagctcggct ccgacatcct
     1621 tccggaagcc gcccgagccg ctccacggac cggtccacgc cgcggccgcg tcgccgtaga
     1681 ggcgggcata caccttgtcg atgtacagga aaagcacctg ccgccgtacg tcgggcagga
     1741 tcgggaacgt atacgccgcc tgcgcccaga gggttttgtt gcctccgagg gcgtagaacg
     1801 gatatccgcg cgcgccggtg agcccgccca cgtagtc
//
LOCUS       DBNHQZ010000398         7181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2826758, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000398 DBNHQZ010000000
VERSION     DBNHQZ010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2826758"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16YU_10845"
     CDS             complement(<1..704)
                     /locus_tag="AC16YU_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065766.1"
                     /translation="MTEPGNPVIAADGLTRRFGTFTAVDAISFEVMPGEILGFLGANG
                     AGKTTAIRMLTGLLAPTGGTASVAGFDVYTGSERIKRTIGYMSQRFSLYEDLTVRENI
                     RFYGGIYGLSAAEIREKTERLVGRLGLGHALDARIRALPLGWRQKLAFSVAVLHGPTI
                     VFLDEPTGGVDPVTRRQFWDLMYEEAERGVTVFVTTHYMDEAEYCDRISIMVAGQIAA
                     IGTPADLKRQHGVDTI"
     gene            complement(697..1608)
                     /locus_tag="AC16YU_10850"
     CDS             complement(697..1608)
                     /locus_tag="AC16YU_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065767.1"
                     /translation="MSAVVATDLVRDFGSTRALDAVSFTVEPGELFGLIGPDGAGKTT
                     LFRILVTLLVPDSGEAFVDGLHVVRDYRELRLRLGYMPGRFSLYPDLSVQENLQFFAT
                     IFGTTVEQNYDLIQDIYGQLEPFRDRRAGALSGGMKQKLALSCALVHRPRVLFLDEPT
                     TGVDAVSRREFWAMLARLKAEGLTIVVSTPYMDEAVRCDRVALIQGGRLLEVDRPDVV
                     AASYDRPLFAVRAGDRLGALRSLRAHPDAHTVYTFGEELHFSAEGAVPDPGKIREYLD
                     RSGIRAEVRRIDAGIEDVFMERMGSHD"
     gene            complement(1622..2542)
                     /locus_tag="AC16YU_10855"
     CDS             complement(1622..2542)
                     /locus_tag="AC16YU_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6065768.1"
                     /translation="MTAMSTNTKWTGAALAALALSVAACTAGDDGSDAFGNFEATEVL
                     VSAEVPGRLVRLAVREGDAVEAGVEVGLVDTLQLALRADQVRAQRAAVRSRLAGITAQ
                     LGVLEEQRRVAERERARFEALLADGAATGKQMDDIEGQMRVLDRQIASARTQYDPIWA
                     EMEVLDAQLATSLDQIRRSRIVNPVAGTVLATYAEPSEMTAAGRPLYKIARLDTLDLR
                     AYVSGAQLPHVRIGQRVTVLIDEDADSERSLEGVVTWIAAEAEFTPKLIQTKEERVSL
                     VYAIRVRVPNPEGRLKIGMPGEVDFASSAN"
     gene            complement(2539..3786)
                     /locus_tag="AC16YU_10860"
     CDS             complement(2539..3786)
                     /locus_tag="AC16YU_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6065769.1"
                     /translation="MTLALLSLLFIAQPDTLTLDRAYRAAAEHYPRAREAGIALAIRD
                     LRVRNLDSNFLPSLSLGGQAVYHTDVASIPMTVPGVELPSVSHDQYRVGLTVDQLVYD
                     GGTTSARRAIEHAAADVAGQEVAVEVYAVREQVEAAWFGSLLGEAEAATLDALAEDVA
                     ARLDLARSYVERGVATGSNAEILEAEQIRIGQQREAALSRSRAARDVLEQLTGWTLVD
                     SARLELPPPGPSVPLSGRRPEIALFELSRRSLDRQADLASRRTRPRVTSFADAAVGRP
                     QGQNMFETEFGPWFSVGVRIGWPVWDWNGSRREREALRLQSDAVTAREEAFEQRVRTA
                     AAQVRREIERLEAHLEGDAAIVGLRARVSAEARSRFENGVITATDYLVERNEEHRARL
                     AESVHRIQLAQARARLATILGAS"
     gene            complement(3783..4388)
                     /locus_tag="AC16YU_10865"
     CDS             complement(3783..4388)
                     /locus_tag="AC16YU_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6065770.1"
                     /translation="MNREHDTEHRIFEAARSVFFEQGFDGARMAEIARRAEINQSMLH
                     YYFRSKDQLFDAVFRKAAGEALPPVVAVLRSDLPMLEKLDRFVNGYIDMIAANPHLPA
                     FILQELRRNPDALRRLVGSATGGVFDGFRAEVRAAVGRGEIRDIAPEHLFANVLALCV
                     FPFIARPMLQTALGLDDPAFDGFIEERRREVSLFIRNALLP"
     gene            complement(4507..5589)
                     /locus_tag="AC16YU_10870"
     CDS             complement(4507..5589)
                     /locus_tag="AC16YU_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6065771.1"
                     /translation="MVRSCIVALLALAGLAGSARAQVDSTRAERPGAKLALLPIIFSS
                     PDTRLALGVLPQYIFHTAPGTRPSNLRADVYYTFNRQFNVLVKPVVWLPGDRWQFAGS
                     FRFRKWPTSFYGIGPGSREADEETFTEYLAHGSVEALRRVRPAVYAGASYVMRWGSIR
                     EVEPVGGLLDRGAVPGTGDSRVAGLGIVATYDTREHVYLPRSGALYRVQVERFARAFG
                     GDRGYGTVTVDLRQYVAVTGPVTAAFQVALAMSDGEVPFRVMPNVGDIVRGYATTRYI
                     DRQRWAAQVEIRAIPVWWRLGLAVFGGAGGVAGRVGDFRTDDLNWAVGIGLRVLMYPR
                     ERITIRQDFAFGQGSSGDYLDLNEAF"
     gene            complement(5713..>7181)
                     /locus_tag="AC16YU_10875"
     CDS             complement(5713..>7181)
                     /locus_tag="AC16YU_10875"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR6065772.1"
                     /translation="ILRVAGVEAEYASLPEADRLRLLERELGQPRPLLSRGATLSPGT
                     DAVLRTFDVIRELVSAEPESVGSVIVSMTHAASDLLEVLLLAKEAGLWDGAGCPLDVV
                     PLFETVDDLERADGLMADLFGNAVYRRHLEGRGRFQEIMLGYSDSNKDGGFWMANWAL
                     HGAQARLVRICRDHEVDFRLFHGRGGTVGRGGGRANRAIASMPAATAGGRIRFTEQGE
                     VISFRYAQQEIAHRHLEQIVSAVLSAQAAAREVGDAHATGEWAEVMDRIARSAMDAYR
                     ALISDGDFWAFYTSSTPIAHISRLPIASRPVSRKKADEVDFEGLRAIPWVFAWTQTRH
                     GVPGWYGTGAGLDAAMEAFGLDTLRAMYAGWPFFAAVIDNLQLEMVRARASVAARYAA
                     LGGDAAGRLHDRIESDLRRAREAVLSITGQDELLDSAPAVQASVRYRDPHTDALNLMQ
                     IELMRRARAERDAGRRERLHELLHRSINGVAAAMQSTG"
BASE COUNT         1106 a         2572 c         2478 g         1025 t
ORIGIN      
        1 tcgatcgtgt cgacgccgtg ctggcgcttc agatcggccg gcgtgccgat ggccgcgatc
       61 tgtccggcga ccatgatcga gatgcggtcg cagtactcgg cctcgtccat gtaatgcgtc
      121 gtgacgaaca ccgtgacccc gcgctccgcc tcctcgtaca tcaggtccca gaactgccgg
      181 cgcgtaacag ggtccacgcc gccggtcggc tcgtccagga agacgatggt cggcccgtgc
      241 agcacggcga cggagaaggc gagcttctgc cgccagccga gcggcagcgc gcggatacgg
      301 gcgtccaggg cgtggccgag gcccagacgg cccaccagcc gctcggtctt ctcccggatt
      361 tccgccgccg acagcccgta gatccccccg tagaagcgaa tgttctcgcg gacggtcagg
      421 tcctcgtaga gagagaaacg ctggctcatg tagccgatcg tgcgcttgat ccgctcggac
      481 ccggtgtaga cgtcgaagcc ggcgacgctc gccgtgccgc cggtgggcgc caggagtccg
      541 gtgagcatgc ggatggccgt cgtcttcccg gctccgttcg cgcccaggaa tccgaggatc
      601 tcgcccggca tcacctcgaa cgagatggcg tccacggccg tgaacgtgcc gaaccggcgc
      661 gtgagaccgt cggccgcgat gacggggttt ccgggctcag tcatggcttc ccatccgttc
      721 catgaacacg tcctcgatgc ccgcatcgat gcgccgcacc tcggcccgga tgcccgaccg
      781 gtccaggtac tcccggatct tgcccggatc ggggacggcc ccctcggcgc tgaaatgcag
      841 ctcctcgccg aatgtgtaca cggtatgggc gtccgggtgc gcccggagcg agcgcagcgc
      901 gccgagccgg tccccggcac ggacggcaaa gagcggccgg tcgtacgagg cggcgaccac
      961 gtcgggccgg tccacctcca gcagccggcc gccctggatc agggccacgc ggtcgcagcg
     1021 cacggcctcg tccatgtagg gcgtggagac cacgatcgtc aggccttccg ccttgagccg
     1081 ggcgagcatg gcccagaact cgcgccgcga gacggcgtcg acccccgtgg tgggttcgtc
     1141 caggaacagg acgcgcggcc ggtgcacgag cgcgcacgac agcgccagct tctgtttcat
     1201 gccgccggag agcgcgcccg ccctgcggtc gcggaacggc tccagctggc cgtagatgtc
     1261 ctggatcagg tcgtaattct gctcgacggt cgtgccgaag atcgtggcga agaactggag
     1321 gttctcctgg acgctcaggt cgggatacag tgaaaaacgc cccggcatgt agcccagccg
     1381 cagccgaagc tcccggtaat cccggaccac gtgaagcccg tccacgaagg cctcgcccga
     1441 gtcgggcacg aggagcgtga ccaggatgcg gaacagcgtc gtcttcccgg ccccgtccgg
     1501 cccgatcagt ccgaacagct cgcccggctc gaccgtgaac gagacggcgt ccagggcgcg
     1561 ggtcgacccg aaatcccgga ccaggtcggt ggcgacgacg gcggacatgg cgggtaccgg
     1621 atcagttcgc ggacgatgcg aagtcgacct cgcccggcat gccgatcttc agccggcctt
     1681 ccggattggg cacgcggacc ctgatcgcgt agaccaggct cacgcgctcc tccttggtct
     1741 ggatgagctt cggggtgaac tcggcctccg ccgcgatcca ggtgacgacc ccctcgaggc
     1801 tccgctcgga gtcggcgtcc tcgtcgatga gcaccgtcac ccgctgcccg atccggacgt
     1861 ggggaagctg cgcgccgctc acgtaggccc gcaggtccag cgtgtcgagc cgcgcgatct
     1921 tgtatagcgg gcggcccgct gccgtcatct cggagggttc ggcgtacgtg gcgagcaccg
     1981 ttcccgcgac cgggttcact atgcggctcc gacggatctg gtcgagcgag gtcgccagct
     2041 gcgcgtccag gacctccatc tcggcccaga tcgggtcata ctgcgtccgg gccgaagcga
     2101 tctgccgatc caggacacgc atctgtcctt cgatgtcgtc catctgcttg ccggtggccg
     2161 cgccgtccgc gagcagcgcc tcgaaccggg cccgctcgcg ctcggccacc cgccgctgct
     2221 cctcgagcac tccgagctgc gcggtgatgc cggcgagccg cgagcgtacg gccgcgcgct
     2281 gagcccgtac ctggtcggcg cggagggcaa gctgaagcgt gtcgacgagg ccgacctcca
     2341 cgccagcctc gaccgcgtca ccttcgcgca cggcgaggcg aacgagccgc ccggggactt
     2401 cggccgagac cagcacctcg gtggcctcga agttgccgaa ggcgtccgac ccgtcgtcgc
     2461 cggccgtgca ggccgcgacg gagagggcga gggcggccag cgcagcgccg gtccatttcg
     2521 tgtttgtgga catcgcggtc atgatgctcc gaggattgtg gccagcctgg cccgggcctg
     2581 ggccagctgg atgcggtgta cggattcagc cagcctggcc cggtgctcct cgttgcgctc
     2641 gaccaggtag tcggtggccg tgatgacccc gttctcgaac cggctccgcg cctcggccga
     2701 gacgcgcgcc cgcagcccta cgatcgccgc gtctccctcc aggtgcgcct cgaggcgctc
     2761 gatctcccgt cggacctggg ccgcggcagt gcggacgcgc tgctcgaagg cctcctcgcg
     2821 ggccgtcacc gcgtcggact ggagccggag cgcctcccgc tcccggcgtg agccgttcca
     2881 gtcccacacg ggccacccga tccggacgcc caccgagaac caggggccaa actcggtctc
     2941 gaacatgttc tggccctgcg gccggcccac ggccgcgtcc gcgaacgagg tgacgcgcgg
     3001 gcgcgtccgt cgggaggcga ggtcggcctg gcggtccagg ctccgtcggg agagctcgaa
     3061 gagcgcgatc tccggccgcc gtccggaaag gggcacggac ggtccgggcg gcggcagctc
     3121 caggcgggcg gagtcgacca gcgtccaccc ggtcagctgc tcgagcacgt cccgcgcggc
     3181 ccgcgagcgg ctcagggccg cctcgcgctg ctgcccgatg cggatctgct cggcctccag
     3241 gatctcggca ttcgagcccg tggcgacccc gcgttccacg tacgagcggg ccaggtccag
     3301 cctcgccgcc acgtcctccg ccagggcgtc cagggtggcc gcttccgcct cgccgagcag
     3361 agagccgaac caggccgcct cgacctgctc gcgcacggcg tagacctcga ccgccacctc
     3421 ctgtccggcg acatcggccg ccgcatgctc gatggccctc cgcgccgacg tcgtgccccc
     3481 gtcgtacacg agctggtcga cggtgagccc cacccggtac tggtcgtggc tcacggaggg
     3541 gagttccacg ccggggacgg tcatcggaat cgacgccacg tcggtgtggt acacggcctg
     3601 cccgcccagc gagagcgacg gcaggaagtt cgaatccagg tttcggacac ggaggtcccg
     3661 aatcgcaagc gcgatgcccg cctcgcgggc gcgtggatag tgttcggccg ccgcgcggta
     3721 ggcccgatcc agcgtcaggg tgtcgggctg cgcgatgaag aggagcgaaa gcagtgccag
     3781 cgtcatggca ggagagcgtt tcggatgaac agggagacct cccggcgccg ctcctcgatg
     3841 aacccgtcga acgcggggtc gtcgagcccc agggccgtct ggagcatggg ccgggcgatg
     3901 aacggaaaga cgcagagcgc gaggacgttc gcgaagaggt gctccggggc gatgtcgcgg
     3961 atctctccgc gcccgaccgc tgcccgcacc tccgcgcgga atccgtcgaa cacgcctccc
     4021 gtcgcggatc cgaccaggcg ccgcaacgca tccgggttgc ggcggagctc ctgcaggatg
     4081 aaggccggca ggtgggggtt cgcggcgatc atgtcgatgt agccgttcac gaagcggtcc
     4141 agcttctcaa gcatcggcag gtccgagcgc aggacggcga cgaccggcgg aagagcctcc
     4201 cccgctgcct tgcggaagac ggcgtcgaac agctggtcct tggagcggaa gtagtagtgg
     4261 agcatcgact ggttgatttc cgcgcgccgg gcgatctcgg ccatccgcgc tccgtcgaag
     4321 ccctgctcga agaagacgga cctggccgcc tcgaaaatcc ggtgttcggt gtcgtgctcc
     4381 cggttcatcg tgggatccgt gggttgcgga ggcaagcata cgacggtttg accatagagt
     4441 tcaactgttt agttgaatta atttgtcgac cgagccaaga tcgcggtgta tcgactccga
     4501 aaggcctcag aaggcctcgt tcaggtccag gtagtccccg ctcgatccct gtccgaacgc
     4561 gaagtcctgc cggatggtga tccgctcccg cgggtacatc aggacgcgga ggccgatgcc
     4621 cacggcccag ttcaggtcat ccgtgcggaa gtccccgacc cggccggcga cgccccccgc
     4681 tccgccgaac acggcaagcc ccaggcgcca ccagacgggt atggcccgta tctcgacctg
     4741 cgccgcccac cgctgccggt cgatgtaccg ggtggttgca tagcccctga ctatatcccc
     4801 gacattcggc atcacgcgga agggcacctc cccgtcgctc atcgcgaggg ccacctggaa
     4861 cgcggcggtc accggtcccg tcaccgcgac gtactgccga agatcgaccg tcaccgtgcc
     4921 gtaaccccgg tcccccccga acgcccgggc gaatcgctcc acctgcacgc gatacagcgc
     4981 gccgctccga ggcaggtaca cgtgctcacg cgtgtcgtac gtggccacga ttccgagccc
     5041 ggccacgcgg ctgtcccccg tgcccggcac cgctccccgg tccagaaggc cgccgacagg
     5101 ctccacctcc cggatgctcc cccagcgcat gacatagctc gccccggcat acaccgccgg
     5161 gcggacccgg cgaagggcct cgaccgagcc atgggccagg tactcggtga aggtctcctc
     5221 gtccgcctcg cgcgagcccg gcccgatgcc gtagaaggac gtgggccact tccggaagcg
     5281 gaacgatccc gcgaactgcc aacggtctcc gggcagccac acgaccggct tcacgagcac
     5341 gttgaactgc cggttgaacg tgtaatagac gtccgcccgg aggttcgagg ggcgggtgcc
     5401 cggcgccgta tggaagatgt actgcggcag gacccccagc gcgagccgcg tgtccggcga
     5461 ggaaaagatg atgggcagga gcgcgagctt cgctcccggc ctttcggcac gggtcgagtc
     5521 gacctgggcg cgggccgatc cggcgagccc cgcgagggcg aggagggcga cgatgcagga
     5581 acggaccatc gcggcacggc ggcttggacc gcggcaggat cggctggcgc atgcacgaca
     5641 gaacggacgc ccgcacccga caaaccggac accgtcggca cgcggatccc gctgtcgcgg
     5701 tagccggaac gctcaccccg tgctctgcat cgcggcggcc acgccgttga tgctccggtg
     5761 cagcagctcg tgcaggcgct cccgacggcc ggcgtcccgc tcggcgcggg cgcgccgcat
     5821 gagctcgatc tgcatcaggt tcagggcgtc ggtgtgggga tcgcggtagc ggacggacgc
     5881 ctgcacggca ggcgccgagt ccaggagctc gtcctggccc gtgatggaaa ggacggcctc
     5941 gcgggcacgg cgcaggtcgg actcgatccg gtcgtgcagg cgcccggccg cgtcgccgcc
     6001 cagcgccgcg tagcgggccg cgacggacgc ccgcgcccgc accatctcga gctgcaggtt
     6061 gtcgatgacg gccgcgaaga agggccaccc ggcgtacatc gcgcgcagcg tgtccaggcc
     6121 gaacgcctcc atcgccgcgt ccagacccgc cccggtgccg taccaccccg gcacgccgtg
     6181 ccgggtctga gtccacgcga acacccacgg aatggcgcgc aggccctcga agtcgacctc
     6241 gtcggccttc ttgcgggaga ccgggcgcga ggcgatgggc aggcgactga tgtgcgcgat
     6301 gggcgtggac gacgtgtaga aggcccagaa gtccccgtcc gagatcagag cgcggtaggc
     6361 gtccatggcg gagcgggcga tccggtccat gacttcggcc cactcgcccg ttgcgtgggc
     6421 gtcgccgacc tcccgcgccg ccgcctgtgc cgagagcacg gcgctcacga tctgctccag
     6481 gtggcggtgc gcgatctcct gctgcgcgta ccggaacgag atcacctcgc cctgctccgt
     6541 gaagcggatg cgtccgcccg cggtcgccgc gggcatcgac gcgatggccc ggttcgcgcg
     6601 gccgcccccc cgacctacgg tgccaccgcg accgtggaag agacggaagt ccacctcgtg
     6661 gtcgcgacag atgcgcacga gcctggcctg cgcgccgtgc agcgcccagt tggccatcca
     6721 gaagccgccg tccttgttcg aatccgagta gccgagcatg atctcctgga agcggccccg
     6781 gccttcgaga tgccgccggt agacggcgtt gccgaagaga tcggccatga ggccgtcggc
     6841 ccgctccagg tcgtcgaccg tctcgaagag cggcaccacg tcgagcggac agccggctcc
     6901 gtcccagagt ccggcctcct tcgcgagcag caggacctcc aggaggtccg acgcggcgtg
     6961 cgtcatgctg acgatcacgg agcccaccga ctccggctcc gccgagacca gctcgcggat
     7021 cacgtcgaac gtgcgcagga cggcgtccgt gccgggcgag agcgtcgcgc cccgggagag
     7081 gagcggccgc ggctgcccga gctcgcgctc gagcaggcga agccggtcgg cttccggcag
     7141 ggaagcgtac tccgcctcga cgccggccac gcgcaggatc t
//
LOCUS       DBNHQZ010000399         7321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2150016, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000399 DBNHQZ010000000
VERSION     DBNHQZ010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2150016"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16YU_10880"
     CDS             complement(<1..448)
                     /locus_tag="AC16YU_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR6065773.1"
                     /translation="MPIRIVIAGAAGRDFHNFNTVYRGRSDTRVVAFTATQIPNIDGR
                     VYPSVLAGPGYPDGIPIVPEDDLERLVTDLEVNCVVFAYSDVTYEHVMRVGSRAMAAG
                     ADFTLLGPRSTMLRSTRPIVSVCAMRTGAGKSQTSRYVARILGDQGL"
     gene            complement(622..1230)
                     /locus_tag="AC16YU_10885"
     CDS             complement(622..1230)
                     /locus_tag="AC16YU_10885"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6065774.1"
                     /translation="MSPAPEPPPTATASRRPWWRSEWVILLAVLAVLHATGTLVYVQA
                     FLQRGLLATGIFSADLLPASGRTPLPPGISFLDADGRSVRLDDLRGEVLFVNLWASWC
                     APCLAEMPAIDRLYETHGDRVRFFMVTLDEDPELGFGHAERSGFSFPVYRPGSAIPSP
                     VYGGVLPTTVVVDGEGRIAVRHEGMARYDSRAFRAILDGLSH"
     gene            complement(1241..3847)
                     /locus_tag="AC16YU_10890"
     CDS             complement(1241..3847)
                     /locus_tag="AC16YU_10890"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR6065775.1"
                     /translation="MTRPTAAALVLCAAFHAPGFAQAPASLPLDGSVPEFRIDRSEIG
                     LQRVARPGTYFDKAGRRFAMLGEESGSFEAWAWPLKLFRDFRLSFFIGESTTPIRAED
                     IVQTVRAEPAATTLTYVFQSFTVRAHFVAHPEEPGGVVLLDVDSTVPLRIVAGFIPAM
                     EPMWPAGLGGQYAFWDGGLKAYLVSESSRRNHGYVGSPSAAGLSYTPAHMLSEQPNEF
                     AIAVDDPGSLRDRFVVIAMAGGPGERQAVREVYERFAADPEAVYRAASHHMRRLLDSS
                     VRVTTPDPLVNRAFAWAAVSYDNLFVRNPHFDGPGLTAGFDRAGRGGRPGFGWFFGGD
                     TYINSLSLNALGSHDTAREAIAFTEAFQREDGKMPHEVTQAREYVDWFGRYGYAYIHA
                     DTSPWFVAAVHDHFLWTADSSWVRARWPALRRAYDWSRATDGNGDGLMDNSLAGLGAL
                     EFGSLTGIETDIYLGAVWIRAAEAMRALARVAGDEETARRADEDFDLATAAFGKFWNG
                     SIGQYSYGFSDEGATVAEVTPWAAVGLMWGYGTDDRARSTLERMARSDMTTDWGVRMM
                     STESPYYEPLNYNYGAVWPFLTSWVATAQYARGLPMRAFSTWRTAIGHVERRALGHVT
                     EVFSGAAHTWPQESVPHQGFCTAATVLPFVRGLLGIAPDAPGGVLTLRPALPADWPAV
                     SVEHLRVGRGTVSIRFGQPSPGRLDVALSRTDGAPAAVRFEPSLPALSRVTGVTVDGA
                     TVPHAIRHAPEATVVSVEVPVADSVRIAVSYEPGFSLVAPAWETEVGDPNRGLRIVSF
                     DASDPSLVDVVLEGLAGRPYEIGVTGADRVESLETDGSVALRGDRLTVQIPGDGPGYA
                     STAVRVHLRPSP"
     gene            complement(3912..5408)
                     /locus_tag="AC16YU_10895"
     CDS             complement(3912..5408)
                     /locus_tag="AC16YU_10895"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR6065776.1"
                     /translation="MSFRSIVALALSIPALAPRAGAQDVPPPRPHLPIPTAAQLAWQR
                     DEMRMFVHFGMNTFTDREWGTGDEDPSTFRPDSLDARQWARVAARTGFRAVVLTARHH
                     DGFALWPTASSRHSVAASPWRGGEGDVVRELADAVAAEGLGLGLYLSPWDRHEPTYGD
                     EEAYNAHYLGQLRELLTGYGPVVEVWFDGAKGEDARDMRYDFAAWWSLVRQLQPGAVL
                     FSDAGPDVRWIGNERGAAGETNWSTYDRSRVGVGMAGIEAYLNTGERGAPDWVPGECD
                     VSIRPGWFHHPGEHPRTLRRLLDLYYSSVGRNCLLLLNVPPAPSGRLDPADVAALDSL
                     ADALRSIFSLNLAAGSRAVASSVHGGSPRFSGERALDGDLDTYWAPGGPGGAPAGSGG
                     ADSLPTEPPYVLTVALPAQASFNVVLLQEPVQLGQRIAGFRVEALTGDAWSVVARGTT
                     VGHKRLLRLAEPVRASAVRIVVEDALALPLIAEFGLFHDPYPAAPLAR"
     gene            complement(5487..>7321)
                     /locus_tag="AC16YU_10900"
     CDS             complement(5487..>7321)
                     /locus_tag="AC16YU_10900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR6065777.1"
                     /translation="ARHAEDRRLELAYHLSEDSATTVRTDRERVAQALQHLMDFTVRH
                     TASGGIVLRTAVDRADSGGLVWNVEFRSSDASADTADLARAFESDDRSSRSSVRMDGG
                     DLAATIGRQLVEQLGGEIWLDPAADGGMSVHAIVPVDPDPDPEAEDRAVLAGLRVLLV
                     DDSEISSAFLAHHLDSAGATVLAADRPAEAVRMVDDEGPIDVIVASLSTARGAAEFVG
                     HLKKIDASQRPPLVLVVPSDLKERSREGRSGSRLGKPVRRSQLVHAVMDAMPDVRSRA
                     AKAEPRIAKARPRIEEAEPRIAESVSGTAGAEPRITGPGPTAGRTERLSVLVAEDDPV
                     NARLMLYLVEGMGHEVTLVRSGDEAVAAFQEREFDAAVMDVRMPGLSGPEAARRILRL
                     CSSTGHQPILVGMTASTASYDREACLSAGMRDCLVKPLTTEVIEEALSGPGRPAAPSA
                     RPRADRPSLPAARHRRTAPDPAAEDDGSAAMETTTDALEEIRRHLDGHYADEPAYAAD
                     LVAAFLRTVPELVDAMEDGLTRGDFDGIRLAARDLKSHCRTIGFARLSALCRTLESVN
                     EEELEPGSLESRVTAVRNTLEAARPALGAEQAELAHLAALHSTL"
BASE COUNT         1081 a         2530 c         2745 g          965 t
ORIGIN      
        1 ggagcccctg atcgccgagg atgcgcgcca cgtaccggct ggtctggctc ttgccggcgc
       61 cggtccgcat cgcgcagacg gagacgatcg gacgggtgga ccgcagcatg gtggagcggg
      121 gcccgagcag ggtgaagtcg gctccggcgg ccatggcgcg ggagccgacg cgcatcacgt
      181 gctcgtacgt gacgtcggag tacgcgaaga ccacgcagtt cacctcgagg tcggtgacga
      241 ggcgctcgag gtcgtcctcg ggcacgatcg gaatgccgtc cgggtagccg ggtccggcca
      301 ggacggacgg gtacacgcgg ccgtcgatgt tcggaatctg ggtggcggtg aacgccacga
      361 cccgtgtgtc ggagcgcccg cggtagaccg tgttgaagtt gtggaaatcc cgtccggctg
      421 cgccggcgat cacgatgcgg atgggcatgg atagggttga gcgggcgcgg cccgggggac
      481 ggcgccgcgt tcggtttgcg gtggcgcaat caagcccgtc accgattccg ggtcaacagg
      541 caaccggcta gggccgttgc cgggggttcc ccgctgccgg tgtgagggga gccgcccgcg
      601 ccatgtgcgg acgggcaagg gtcagtgcga gagtccgtcg aggattgcgc ggaaggcacg
      661 cgagtcgtac ctggccatgc cctcgtgcct cacagcaatg cggccttcgc cgtccacgac
      721 cacggtcgtc ggaagcacgc cgccgtacac cgggctgggg atcgcgctgc ccgggcgata
      781 caccgggaac gagaatccgg accgctcggc atggccgaat ccgagctccg ggtcctcgtc
      841 gagcgtgacc atgaagaacc gcaccctgtc tccgtgcgtc tcgtacaggc gatcgatcgc
      901 gggcatctcg gcgaggcagg gggcgcacca gctggcccac aggttcacga agaggacctc
      961 gccgcgcagg tcgtccagcc gcacgctgcg gccatccgcg tccaggaagg agatgccggg
     1021 cggaagggga gtgcgccccg acgcgggcag taggtcggcc gaaaagatgc ccgtcgcgag
     1081 caggccgcgc tgcaggaagg cctggacgta cacgagcgtt ccggtcgcgt gaaggacggc
     1141 gaggacggcc agcaggatca cccactcgct gcgccaccag gggcggcggg aggcggtcgc
     1201 ggtcggaggg ggttcgggcg cggggctcat tcggtcgcgg tcagggggac ggcctcaggt
     1261 gcacgcggac ggccgtggac gcgtacccgg ggccgtcgcc cgggatctgc accgtgagcc
     1321 ggtcgccgcg cagcgcgacg ctgccgtccg tctcgagcga ctcgacccgg tcggcgccgg
     1381 tgaccccgat ctcgtacggc cgcccggcga gcccctccag gaccacgtcg acgagggacg
     1441 ggtcggacgc gtcgaacgaa acgatccgga ggccccggtt cggatctccg acctccgtct
     1501 cccaggccgg cgcgacgagc gagaaacccg gttcgtagga aacggcgatc cggaccgaat
     1561 cggcgacggg gacctcgacc gacacgacgg tcgcctcggg cgcgtggcgg atcgcgtggg
     1621 gcaccgtggc gccgtcgacc gtgacgccgg tcacccggct gagcgcgggc agggacggct
     1681 cgaagcgcac ggcggcgggg gcaccgtccg ttcgggacag cgcgacgtcg agccgcccgg
     1741 gcgagggctg tccgaaccgg atcgagacgg tgccccggcc cacgcgcagg tgctcgacgc
     1801 tcacggccgg ccagtcggcg ggcagcgcgg gccggagcgt gagcacgccg ccgggcgcgt
     1861 ccggggcgat gccgagaagg ccgcgcacga agggcaggac cgtcgcggcc gtgcagaacc
     1921 cctggtgcgg caccgactct tggggccagg tgtgcgcggc gcccgagaag acctcggtca
     1981 cgtgcccgag cgcgcggcgc tcgacgtgtc cgatggccgt tctccacgtg ctgaaggccc
     2041 gcatcggaag gccgcgggcg tactgggctg tcgccaccca gctcgtgagg aacggccaga
     2101 cggccccgta attgtagttc agcggctcgt agtacgggct ctccgtggac atcatgcgga
     2161 cgccccagtc ggtcgtcatg tcggaccggg ccatgcgctc gagcgtggaa cgcgcccggt
     2221 cgtccgtgcc gtacccccac atcaggccca cggccgccca cggcgtcacc tcggcgacag
     2281 tcgcaccctc gtccgagaag ccgtagctgt actggccgat ggacccgttc cagaacttcc
     2341 cgaaggccgc agtcgcaagg tcgaaatcct cgtccgcccg ccgggccgtt tcctcgtctc
     2401 cggcgacgcg tgcgagcgcg cgcatggcct cggcggcgcg gatccacacg gctccgagat
     2461 agatgtccgt ctcgattccg gtcagcgagc cgaactccag cgcgccgagg cccgcgagcg
     2521 agttgtccat gagcccgtcg ccgttgccgt cggtggcccg cgaccagtcg taggcgcggc
     2581 gcagggcggg ccagcgcgca cggacccatg aggagtccgc cgtccacagg aaatggtcgt
     2641 ggacggccgc gacgaaccag ggggaggtgt ccgcgtggat gtacgcatag ccgtaccggc
     2701 cgaaccagtc gacgtactct ctcgcctgcg tcacctcgtg cggcatcttg ccgtcctcgc
     2761 gctggaacgc ctcggtgaac gctatcgcct cgcgcgccgt gtcgtggctg ccgagcgcgt
     2821 tcaggctgag cgagttgatg tacgtgtccc cgccgaagaa ccagccgaag cccggccggc
     2881 cgccccgtcc ggcgcggtcg aagccggccg tgaggccggg cccgtcgaaa tgcggattcc
     2941 ggacgaagag gttgtcgtac gagacggcgg cccacgcgaa ggcgcggttc acgagcggat
     3001 cgggcgtcgt gacgcggacg gaggagtcca ggaggcgccg catgtggtgg gaggcggccc
     3061 ggtataccgc ctccgggtcg gcggcgaagc gctcgtagac ctcgcgcacg gcctggcgct
     3121 cgcccggccc gcccgccatc gcgatcacca cgaaccggtc ccggaggctc ccggggtcat
     3181 cgacggcgat cgcgaactcg ttcggctgct ccgagagcat gtgggcgggc gtgtaggaga
     3241 gtcccgccgc cgacggcgag cccacgtaac cgtggttgcg gcgcgaggac tcggagacga
     3301 ggtaggcctt gaggccgccg tcccagaagg cgtattggcc cccgagcccg gcaggccaca
     3361 tgggctccat ggcggggatg aacccggcca cgatgcggag gggcacggtc gagtcgacgt
     3421 ccagcagcac gacgccgccc ggctcctccg gatgggccac gaagtgcgcc cggacggtga
     3481 aggactggaa gacgtacgtc agggtggtcg ccgccggctc cgcgcggacg gtctgcacga
     3541 tgtcctcggc ccggatcggg gtcgtgctct cgccgatgaa gaacgagaga cggaaatccc
     3601 ggaacagctt caggggccag gcccaggcct cgaacgagcc gctctcctcg cccagcatcg
     3661 cgaaccggcg ccccgccttg tcgaagtacg tgccgggccg ggccacgcgc tgaaggccga
     3721 tctccgagcg gtcgatgcgg aactcgggga ccgagccgtc cagcggcagg gaggccgggg
     3781 cctgggcgaa tccgggggcg tggaaggccg cgcagagaac gagcgcggcg gcggtcgggc
     3841 gtgtcatggc ggccgtcggt ggatccacgg cgagtatacg ccgatccccg cacaaagcgg
     3901 ccggggacga gtcagcgggc caggggcgcg gccgggtacg gatcgtggaa caggccgaat
     3961 tccgcgatga gcggcagcgc gagcgcatcc tccacgacga tccggaccgc gctcgcccgg
     4021 acgggctccg ccaggcggag gagcctcttg tggccgacgg tggtcccccg cgcgacgaca
     4081 ctccacgcat cgcccgtcag cgcctcgacc cggaagccgg cgatccgctg cccgagctgg
     4141 acaggctcct ggagcaggac gacgttgaag gatgcctgtg cagggagcgc cacggtgagc
     4201 acgtacggcg gctccgtcgg gagggaatcc gccccgccgc tcccggccgg cgctccgccc
     4261 gggccgcccg gagcccagta cgtgtcgagg tcgccgtcga gcgcccgctc tcccgagaac
     4321 cgcggcgaac cgccgtgcac ggacgaggcg acggccctgg acccggcggc gaggttcagc
     4381 gagaagatgc tccgaagggc atccgcgagc gaatcgaggg ccgcgacatc ggccgggtcc
     4441 aggcggcccg acggcgccgg cggcacgttc agcaggagca ggcagttccg gccgaccgac
     4501 gagtagtaca ggtcgagcag ccggcggagc gtgcggggat gctcgcccgg gtggtggaac
     4561 cagcccgggc gtatggacac gtcgcactcg cccggcaccc agtccggggc gccgcgctcg
     4621 cccgtgttca ggtacgcctc gatgccggcc atcccgacgc cgacccggct ccggtcgtag
     4681 gtggaccagt tggtctcgcc ggccgccccg cgctcgttgc cgatccagcg gacatcgggg
     4741 cccgcatccg agaacaggac cgccccgggc tgcagctggc gtacgagcga ccaccaggcg
     4801 gcgaagtcgt agcgcatgtc ccgcgcatcc tcgcccttcg caccgtcgaa ccagacctcc
     4861 accacggggc cgtagccggt gagcagctcg cgcagctggc ccaggtagtg agcgttgtag
     4921 gcctcctcgt cgccgtaggt cggctcgtgc cggtcccagg gggagagata aagcccgagc
     4981 cccaggcctt ccgcggccac cgcgtcggcg agctcccgca ccacgtcgcc ctcgccgccg
     5041 cgccagggcg atgccgcgac ggagtggcgc gacgaggccg tgggccagag ggcaaaaccg
     5101 tcgtggtgcc tggcggtcag cacgacggcg cggaagccgg tccgtgcggc cacgcgggcc
     5161 cactggcggg catcgaggga atccggtcgg aaggtggacg gatcctcgtc gcccgttccc
     5221 cactcgcggt cggtgaaggt gttcatcccg aaatggacga acatccgcat ctcgtcccgc
     5281 tgccaggcca gctgcgccgc ggtcgggatc ggcaggtgcg ggcggggcgg ggggacgtcc
     5341 tgcgcccccg cgcgtggcgc gagggccggg atggacaggg cgagggcgac gatcgaccgg
     5401 aaggacacga cagtgggggt cttgtcgccc aaaatagctg cggcacgccg tgcgtggtac
     5461 cgtccccgca accccgccgg cgagcgtcag agcgtcgagt gcagggcggc caggtgcgcg
     5521 agttcggcct gctctgcgcc gagtgccggc ctggcagcct cgagcgtatt acggacggcc
     5581 gtgaccctgg attccaggga acccggctcc agctcctctt cgttcaccga ttcgagcgtt
     5641 cggcacagcg cggacaggcg ggcgaacccg atcgtccggc agtgggactt gagatcccgg
     5701 gcggccaggc ggatgccgtc gaagtcaccg cgggtgaggc cgtcttccat cgcgtcgacg
     5761 agttcgggca cggtacgcag gaaggcggcg accaggtcgg cggcatacgc cggctcgtcc
     5821 gcgtagtgcc cgtccaggtg ccggcggatc tcctcgaggg cgtccgtcgt ggtttccatt
     5881 gccgccgaac cgtcgtcttc cgcggccggg tccggagcgg tgcggcgatg ccgtgcggcc
     5941 ggaaggctcg ggcggtcggc cctgggccgg gcgctcggcg cggccggccg accgggtccg
     6001 ctgagagcct cctcgatcac ctcggtggtg agcggcttca ccaggcagtc gcgcatgccg
     6061 gccgacaggc aggcctcgcg gtcgtaggac gccgtcgagg ccgtcatgcc gacgagaatc
     6121 ggctggtgcc cggtcgacga gcagagacgc aggatgcggc gcgcggcctc gggtccggag
     6181 agcccgggca tgcgcacgtc catgacggcg gcgtcgaatt cgcgctcctg gaaggcggcg
     6241 acggcctcgt ctccggatcg gaccagggtc acctcgtgcc ccatgccctc caccaggtac
     6301 agcatgagcc gcgcgttcac gggatcgtct tcggcgacga ggacggagag ccgctccgtc
     6361 cgaccggccg tcggcccggg cccggtgatc ctgggctccg cccctgcggt tccggatacg
     6421 gactcggcga tccggggctc ggcctcctcg atcctgggcc tggccttcgc gatccggggc
     6481 tcggccttcg cggcccggga acgcacgtcg ggcatcgcgt ccatgaccgc gtgcaccagc
     6541 tggctccgcc gtacgggctt gcccagcctg gagcccgagc ggccttcgcg ggaccgctcc
     6601 ttcaggtcgg acgggacgac gagcaccagg ggcggacgct gtgatgcgtc gatcttcttc
     6661 aggtgcccca cgaattccgc cgcgccgcgc gcggtggaca ggctcgcgac gatgacgtcg
     6721 atgggtccct cgtcatccac catgcgcact gcctccgcgg gacgatccgc ggccagtacc
     6781 gtcgctcccg cgctgtcgag gtggtgggca aggaaggcgc tcgagatctc gctgtcgtcg
     6841 acgaggagaa cgcgcaggcc ggccaggacg gcccggtcct cggcctcggg gtcgggatcg
     6901 gggtcgaccg gcacgatggc gtgtaccgac attccgccgt ccgcggcggg atcgagccag
     6961 atctcgccgc ccagctgctc gacgagctgg cggccgatcg tggccgcgag gtcacctccg
     7021 tccatgcgca cggaggatcg ggaggaccgg tcgtccgact cgaacgcgcg cgccaggtcg
     7081 gccgtgtccg cggacgcgtc cgagctgcgg aactcgacgt tccatacgag tccgcccgaa
     7141 tcggcgcggt cgaccgctgt ccgcagcacg atgcctcccg atgcggtgtg gcggaccgtg
     7201 aaatccatga ggtgctgcag ggcctgggcg acgcgctcgc ggtccgtccg cacggtcgtc
     7261 gcggagtcct cggacaggtg gtaggccaac tcgagccgcc gatcctcggc gtggcgcgcc
     7321 g
//
LOCUS       DBNHQZ010000400         7603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3506273, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000400 DBNHQZ010000000
VERSION     DBNHQZ010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3506273"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16YU_10905"
     CDS             <1..329
                     /locus_tag="AC16YU_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HIR6065778.1"
                     /translation="IADVDVGDNIGAKLQTDQAEADLKIARAKAEERRASAVASEQEM
                     RAAVEEQRARVVAAEAEIPLAIAEAFRSGNLGVMDYYNLRNIQADTRMRDTIGGPDTP
                     KSPVQE"
     gene            356..571
                     /locus_tag="AC16YU_10910"
     CDS             356..571
                     /locus_tag="AC16YU_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065779.1"
                     /translation="MTGTVRHLAFEGGAWGIEADGGEKLLPLNLDERWRVDGLRIRFR
                     ASPASVLGVIQWGHAVRVSDVRPADDP"
     gene            568..1110
                     /locus_tag="AC16YU_10915"
     CDS             568..1110
                     /locus_tag="AC16YU_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065780.1"
                     /translation="MTDPDRLAGIVSFATVGAVTALQELAARVLSGVEGADPELVAEE
                     TLALVCTTTARAASFALSPSPALAAAAGAALLDLPLTYHDYLIGNSILDGGPTGDAAG
                     HAEIYRRLERKMSFYAAHLQEGALPGPRALSDVMALWMGRVSPPRLPEMPQQRLERLE
                     LVDVLVRHAALVRSHAGREA"
     gene            complement(1117..2394)
                     /locus_tag="AC16YU_10920"
     CDS             complement(1117..2394)
                     /locus_tag="AC16YU_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6065781.1"
                     /translation="MLYVVLIVLTLLLSAFFSGSEIAFVTVNRLRVEVLARRGGVVGP
                     IVQRFLKEPATLLTTTLVGNNLSLVVYSTLLAVYLQEPLSRFAGETLGMAQPGAEALI
                     LLAQTVIGSAVVLVFGEIIPKSLVREVATRAVFALAVPLRITYFLLLPLIKVSGWTAS
                     VLIRFVRADADTFSQFMRRDFELMLEESKKSGELDLDEEESELLSNVFAMGSIRVKES
                     MVPRTDIVAVEVSTPVEDLRQVFVRSGHSKLPVYRDNIDRIVGMAFAYDLFSEPASVE
                     SMMREVRFVPDTKLSKDLLREFLAANTSIAIVIDEYGGTAGLVTTEDLLEELFGDIQD
                     EFDNEIQEMRRLDDNTIVVSGRVDIDELEEKLGWALPEGDYETVAGYLLDRLGTIPAV
                     RDEVLVDGVRFTILQATANRIELVRARREPAQA"
     gene            complement(2403..3203)
                     /locus_tag="AC16YU_10925"
     CDS             complement(2403..3203)
                     /locus_tag="AC16YU_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6065782.1"
                     /translation="MTNAHLMAFDTLLLETGDDGIAVVTVNRPDKLNALNASVLDDLD
                     ACFAALATDDAVRGILITGAGDRSFVAGADIAQFADLDPLGAAAFARRGQAVFDRIEH
                     CPKPVIAVIGGFALGGGCELALACHLRVASTAARLGQPEVGLGLIPGYGGTQRLPRVV
                     GRGLAVEMILTGAQVTADRAYAIGLVNRVFEPDVLLAEARSFLATILRQAPVAVSLAL
                     EAIRASDQAQPDGMALEAALFGQAFATEDVREGVGAFLERRKPSFTGR"
     gene            complement(3200..4069)
                     /locus_tag="AC16YU_10930"
     CDS             complement(3200..4069)
                     /locus_tag="AC16YU_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065783.1"
                     /translation="MPQPEINYTKEAFLHPWNLTFLIVSMLSAFAVSLTGVSWPFETV
                     LIFAAAAELMYLGYMPRQERFRLAVRSRRASEHAKPPSQKEIFQLLTKYSQRRYARQR
                     KLEKDIQSNYRKLSYASQGMLDSHLQKIDGLLDSYLNLLYQKERYEYTASTTKEGEVV
                     RAIKALREDMEDDTPRVRAIKQRRLRILEQRLERSKKSAENLQIIEAQLETIEDVVLY
                     IHEQSLTLRNPEEITFQLDTLLSEVEETQASVEAIEEVFAQPADLLSDVEGFDLPVED
                     SESRTRERGAERS"
     gene            complement(4126..4608)
                     /locus_tag="AC16YU_10935"
     CDS             complement(4126..4608)
                     /locus_tag="AC16YU_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065784.1"
                     /translation="MAAMSPEEYERLKEAEKEHLRKLRQLKRAVHELERQKKVSRAVH
                     DMTEEPRRLLDEQEDLVNQIAIEAAQSEARLDIALESAEAKAPEEPAGISAEEADEVL
                     RRARARELVRRMQEGEDVEPKVPDRAASSAPKPASKEAGGEEDGPPPLPEKTIGRMKP
                     "
     gene            complement(4614..5384)
                     /locus_tag="AC16YU_10940"
     CDS             complement(4614..5384)
                     /locus_tag="AC16YU_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR6065785.1"
                     /translation="MSLWARFKRLMRSIFGGAISAIEDPKLILEQNIRELNDQVPKMN
                     ENIATVKANVMLLQKELRKYEIEIVDLTAKIKAGIQAGRDDIAESFALRLEAARDSME
                     RTREQLKYATAAYDKALQVKKAFMREKERKIGEAKEALRAHERAKWQARVADTLESFE
                     VAGMDATHDEMLTRIQEQTARNEARMELALDSVDTESLKIEEDAQKIRASEIVKQFKL
                     EMGVGDTSGAQAEPSVELPERESESGGKTVGRQRTSNE"
     gene            complement(5478..6809)
                     /locus_tag="AC16YU_10945"
     CDS             complement(5478..6809)
                     /locus_tag="AC16YU_10945"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR6065786.1"
                     /translation="MNAVDAIVRKRNGEALPAGLIRELVEGYVDGRVADYQMSAFLMA
                     ALLRGLDAGETAALTEAMLHSGTVLDLSGIPGAKIDKHSTGGVGDKVSLVVAPLAAAC
                     GVVVPMISGRGLGHTGGTLDKLESIPGFRTDLDIPAMLRQLREIGVAMIGQTDDIAPA
                     DRGLYALRDVTGTVESIPLIAASVMSKKLAAGLDGLVLDVKTGGGAFMPDEADARRLA
                     ETLVGVGEAYGCRTVALLTSMDAPLGRTAGNWPEVAESIRCLRGGGPRDLERLSVELT
                     AEMLLLAGRAGDVEAARERARRALADGSALERFRSLVRAQGGDPAVVEDPDARPGSAP
                     VHEVVSDSEGVVQALDARLIGEACVAMGAGRGRKEDGVDSVAGVTLNKTVGDPVGRGD
                     VLARLHGSDADRVRGVAGAVASAFRLGPGVPAAAPLLRGRYASGAWSAVAD"
     gene            complement(6925..7134)
                     /gene="rpmE"
                     /locus_tag="AC16YU_10950"
     CDS             complement(6925..7134)
                     /gene="rpmE"
                     /locus_tag="AC16YU_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR6065787.1"
                     /translation="MKKDLHPDYHFVTVQLADGTEIKTRSTIEGDRHSSEVDSTNHPF
                     YTGRRQYVDTAGRVEKFMRRYGPKQ"
     gene            complement(7225..>7603)
                     /locus_tag="AC16YU_10955"
     CDS             complement(7225..>7603)
                     /locus_tag="AC16YU_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6065788.1"
                     /translation="FFQYTEKDTKPERTLFIEHGRPLVYANGTRGLRLEGFHLVSVDL
                     QDGPWSADDCLVYDETSFELASIAGRLFRRDDMPQPFGVFYRVERPTYEDQMHAQIAE
                     ITDRSGKGRLDDLLRAGDTWVVS"
BASE COUNT         1131 a         2650 c         2591 g         1231 t
ORIGIN      
        1 acatcgccga cgtggacgtc ggtgacaaca tcggagcgaa gctgcagacg gaccaggccg
       61 aggccgacct gaagatcgcg cgcgccaagg ccgaggaacg gcgggcgtcc gccgtcgcga
      121 gcgagcagga gatgcgcgcc gccgtcgagg agcagcgcgc ccgggtggtc gccgccgagg
      181 ccgaaatccc gctggctatc gcggaggcct tccggtcggg caacctcgga gtgatggact
      241 actacaacct caggaacatc caggccgaca cccggatgcg ggataccatc ggcggcccgg
      301 acacgcccaa gagcccggtc caggagtgac ggccggcgac gcggaggacc ctgccatgac
      361 gggaacggtc cggcacctcg ccttcgaggg cggcgcgtgg ggcatcgaag ccgacggcgg
      421 cgagaagctc ctgccgctga atctggacga gcgttggcgc gtggacggcc tgcgcatccg
      481 gttccgcgcg agcccggcgt ccgtgctcgg cgtgatccag tggggccacg ccgtgcgcgt
      541 gtccgatgtc cgacccgcgg acgatccatg acggacccgg atcggctcgc cggcatcgtc
      601 tcgttcgcca ccgtgggagc agtcaccgcg ctccaggaac tcgccgcgcg cgtgctcagc
      661 ggcgtcgagg gcgcggaccc ggagctcgtc gccgaggaga ctctcgcgct ggtgtgcacg
      721 acgaccgccc gggcggcctc gttcgcgctc tccccgtcgc cggcgctcgc cgcggccgcg
      781 ggcgccgcgc tgctggacct gccgctgacc tatcacgact atctcatcgg caactccatc
      841 ctggacggcg gcccgaccgg cgacgcggcc ggccatgccg agatctaccg gcgcctcgag
      901 cgcaagatgt ccttctacgc ggcccacctc caggagggcg cgcttcccgg gccacgcgcc
      961 ctgtcggacg tcatggccct gtggatgggc cgcgtgagcc cgccccggct cccggagatg
     1021 ccacagcagc gcctggagcg actggaactg gtggacgtcc tggtccgcca cgcggccctc
     1081 gtgcgctcgc acgcgggacg cgaggcctga gacaggtcac gcctgggcgg gctcccgccg
     1141 cgcgcgcacc agctcgatcc ggttggccgt cgcctggagg atcgtgaacc ggacgccgtc
     1201 cacgaggacc tcgtcgcgga cggccgggat cgtgcccagg cgatcgagca ggtagccggc
     1261 cacggtctcg tagtcaccct cgggaagcgc ccatccgagc ttttcctcga gctcgtcgat
     1321 gtccacccgg cccgagacca cgatcgtgtt gtcgtccagg cggcgcatct cctggatctc
     1381 gttgtcgaac tcgtcctgga tgtccccgaa gagctcctcg agaaggtcct ccgtggtgac
     1441 gaggcccgcg gtgcctccgt actcgtcgat cacgatcgcg atcgaggtgt tcgcggccag
     1501 gaattcgcga agcaggtcct tcgacagctt ggtgtccggt acgaaccgta cctcgcgcat
     1561 catcgactcc accgacgcgg gctcgctgaa caggtcgtac gcgaaggcca tgcccacgat
     1621 gcggtcgatg ttgtcccggt acacgggcag cttggaatgc cccgaccgca cgaaaacctg
     1681 ccgcaggtcc tcgaccgggg tcgacacctc gacggccacg atgtcggtgc gcgggaccat
     1741 ggattccttg acgcggatgg accccatggc gaatacgttc gaaaggagct ccgactcctc
     1801 ctcgtccagg tccagctccc cgctcttctt ggactcctcg agcatgagct cgaaatcgcg
     1861 ccgcatgaac tgggagaagg tgtcggcgtc cgcccgcacg aagcggatca gcacggacgc
     1921 agtccatccg gacaccttga tcagcggcag gagcaggaag tacgtgatgc gcagcgggac
     1981 ggcgagggcg aacaccgcgc gggtcgccac ctcccggacc agggacttgg ggatgatttc
     2041 cccgaagacc agcacgacgg cggacccgat cacggtctga gcgagcagga tcagcgcttc
     2101 ggccccgggc tgcgccatgc cgagcgtttc gccggcaaac cggctcaggg gctcctgcag
     2161 gtataccgcg agcagcgtcg agtagacgac gagcgaaagg ttgttgccaa ccagcgtcgt
     2221 ggtcaggagc gtggcgggct ccttcaggaa ccgctgtacg atcggcccca ccacgcctcc
     2281 gcgccgcgcc agcacctcga cccggagcct gttgaccgtg acgaacgcga tctccgatcc
     2341 cgagaagaat gcgctcagga gcagcgtcag gacgatgagg accacgtaga gcatcgacct
     2401 gttcagcgcc cggtgaacga cggttttcgc cgctccagga acgccccgac cccttcacgc
     2461 acgtcctctg tggcgaaagc ctggccgaag agcgccgcct cgagggccat gccgtcgggc
     2521 tgggcctggt cggaggcgcg gatggcctcg agagcgagcg aaacggcgac cggagcctgc
     2581 cggaggatgg tcgccaggaa cgaacgggcc tcggccagga ggacgtccgg ctcgaacacg
     2641 cggttcacga ggccgatggc gtacgcacgg tcggcggtga cctgggcgcc cgtcaggatc
     2701 atttcgacgg cgagcccccg gcccacgacc cgcggcaggc gctgcgttcc gccgtagccc
     2761 gggatgaggc cgagcccgac ctcgggctgg ccgagccggg ccgccgtgga cgcgacgcgc
     2821 aggtggcagg cgagcgcgag ctcgcagccc ccgcccagcg cgaagccacc aatgaccgcg
     2881 atgaccggct tcgggcagtg ctcgatgcgg tcgaacacgg cctgtccgcg gcgcgcgaac
     2941 gccgccgccc cgagcgggtc gaggtcggcg aactgcgcga tgtccgcgcc ggcgacgaag
     3001 ctgcggtcgc ccgccccggt gatgaggatg ccgcggacgg cgtcgtccgt cgcgagcgcc
     3061 gcgaagcacg cgtccaggtc gtccagcacc gatgcattca gggcgttgag cttgtccgga
     3121 cggttgaccg tcaccacggc aatgccgtcg tcaccggtct cgagaagcag ggtatcgaag
     3181 gccatgaggt gagcgttcgt cacgagcgct cggcaccccg ctcccgcgtc cgggactcgg
     3241 agtcctcgac gggcagatcg aagccctcga cgtcggagag caggtccgcc ggctgggcga
     3301 agacctcttc gatggcctcg accgatgcct gcgtctcctc gacctcggac agcagggtgt
     3361 cgagctggaa cgtgatttct tcgggattgc gcagcgtcag ggactgttcg tggatataga
     3421 ggaccacgtc ctcgatcgtc tcgagctgcg cctcgatgat ctgcaggttc tccgcgctct
     3481 tcttggagcg ctccagccgc tgctcgagga tccggagccg gcgctgcttg atggcccgaa
     3541 cgcgcggcgt gtcgtcctcc atgtcctcgc gcagggcctt gatcgcacgc acgacctcgc
     3601 cctccttcgt ggtgctggcc gtgtactcgt agcgctcctt ctggtagagc aggttcagat
     3661 aggagtccag gagtccgtcg atcttctgca ggtggctgtc cagcatgccc tgggaggcgt
     3721 agctgagctt gcggtagttc gactggatgt ccttctcgag cttgcgctgc cgtgcgtagc
     3781 ggcgctggct gtacttggtg agcagctgga agatctcctt ctgcgagggc ggtttcgcgt
     3841 gctcggacgc cctgcgggac cggaccgcga ggcggaagcg ctcctggcgc ggcatgtacc
     3901 ccaggtacat gagctccgcc gccgcggcga agatgagcac cgtctcgaac ggccacgaca
     3961 cccccgtcag gctcacggcg aacgccgaga gcatggagac gatcaggaac gtgaggttcc
     4021 aggggtggag gaaggcctcc ttggtgtagt tgatctccgg ctgcggcatt gaggattcgg
     4081 gtcgcttccg gctgggcttc cggggcgatg cggcgcgtgc gccgatcagg gtttcattcg
     4141 accgatggtc ttttccggaa gcggaggggg gccgtcttcc tctccgccgg cttccttcga
     4201 tgccggcttc ggggcggagg atgccgcgcg gtccggcacc ttcggctcga cgtcctcgcc
     4261 ttcctgcatg cgccggacca gctcccgggc ccgcgcgcgg cgcaggacct cgtcggcttc
     4321 ctcggcactg atgcccgcgg gctcctcggg cgccttcgcc tcggccgact ccagggcgat
     4381 gtccaggcgg gcctcgctct gcgcggcctc gatcgcgatc tggttcacca ggtcctcctg
     4441 ctcgtcgagc aggcgccgcg gttcctcggt catgtcgtgg accgcgcggc tcaccttctt
     4501 ctgacgctcc agttcgtgga cggccctctt gagttggcgc aacttcctga ggtgctcctt
     4561 ctcggcctcc ttcaaccgct cgtattcctc gggggacatg gcggccatcg cggtcactcg
     4621 ttggacgtgc gctgccggcc gacggtcttc ccgccggact ccgactcgcg ctcgggcagc
     4681 tcgaccgacg gctcggcctg ggccccggag gtgtccccga cgcccatctc gagcttgaac
     4741 tgcttcacga tctccgaggc gcggatcttc tgcgcgtcct cctcgatctt gagcgactcg
     4801 gtgtcgacgg aatcgagagc gagctccatc ctggcctcgt tcctggcggt ctgctcctgg
     4861 atgcgggtga gcatctcgtc gtgcgtggcg tccatgccgg cgacctcgaa gctctcgagc
     4921 gtatcggcga cgcgggcctg ccacttggcc ctctcgtggg cccgcagcgc ctccttggcc
     4981 tcgccgatct tgcgctcctt ctcgcgcatg aaggccttct tgacctgcag ggccttgtcg
     5041 taggccgcgg tcgcgtactt gagctgctca cgcgtccgct ccatggaatc gcgcgcggct
     5101 tcgaggcgaa gtgcgaagga ctccgcgatg tcgtcgcggc ccgcctggat gcccgccttg
     5161 atcttggcgg tcaggtccac gatttcgatt tcgtatttcc tgagctcctt ctggagaagc
     5221 atcacgttgg ctttcaccgt cgcgatgttc tcgttcatct tgggcacctg gtcgttcagc
     5281 tcccggatgt tctgctccag gatcagcttg ggatcctcga tagccgagat cgcgccgccg
     5341 aagatggagc gcatgagtcg tttgaagcgg gcccagagag acatggcagt gcgagcgttt
     5401 cgttgcggag aatccgccgt attcgatgaa tatacggaag ggtccctgaa gggatacgcg
     5461 cggaggggcg cgacggttca gtcggcgaca gccgaccagg cgcccgaggc gtagcggccc
     5521 ctcaggaggg gcgcagccgc cggcacaccc ggacccaggc ggaaggccga agctaccgca
     5581 ccggccaccc cccgcacgcg atcggcatcc gatccgtgaa ggcgggcgag gacgtcgccg
     5641 cgtcctacgg gatcaccgac ggttttgttg agggtgacgc cggcaacgct gtccaccccg
     5701 tcttcctttc gcccgcggcc ggcgcccatg gccacacagg cctcgccgat gaggcgggcg
     5761 tccagggcct ggaccacgcc ctccgaatcc gagacgactt cgtgcacggg cgccgatccg
     5821 gggcgggcgt ccgggtcttc gacgaccgcc gggtcgccgc cctgcgcgcg gacgaggctc
     5881 cggaagcgct ccagggccga gccgtccgcg agcgcccggc gcgcacgttc ccgcgcggcc
     5941 tcgacgtcgc ccgcccgccc ggcgagcagg agcatctcgg ccgtgagctc gaccgagagg
     6001 cgttccaggt cccgtgggcc cccgccgcgc aggcaacgga tcgactcggc cacctccggc
     6061 cagtttccgg ccgtgcggcc cagcggagcg tccatcgagg tgagcagagc gaccgtccgg
     6121 cagccgtacg cctccccgac tccgacgagc gtctccgcga ggcggcgggc gtccgcctcg
     6181 tccggcatga aggctccgcc gcccgtcttg acgtcgagca cgagcccgtc caggccggcg
     6241 gccagcttct tgctcatgac ggaggcggcg atgagcggaa tgctctcgac cgtccccgtc
     6301 acgtcgcgca gcgcgtacaa tccgcggtcg gcgggcgcga tgtcgtccgt ctgcccgatc
     6361 atggccacgc cgatttcgcg gagctgccgt agcatggcgg ggatgtccag gtcggtgcgg
     6421 aatcccggga tcgactcgag cttgtccagg gtccctcccg tgtggccgag tccccgcccg
     6481 gagatcatgg ggacgacgac gccgcacgcc gcggccagcg gggccacgac gagcgacacc
     6541 ttgtcgccca cgccgcccgt cgaatgcttg tcgatcttgg cgccggggat tcccgacagg
     6601 tccagcaccg tgccggaatg cagcatggcc tcggtgagcg cggccgtctc gccggcgtcg
     6661 agtccgcgca agagcgccgc catcaggaag gcgctcatct ggtagtcggc cacgcgcccg
     6721 tccacgtacc cctcgaccag ctcgcggatc aggccggcag ggagggcctc cccgttccgc
     6781 ttgcgcacga tggcgtccac cgcgttcatg cgcgcgctcc ccgggccgcc gcaccgaccg
     6841 gcgccgcccg gaaacgacga agggcgatcc gctctcgccg accgcccttc cctaccgcca
     6901 tgcaggccga ccgtgcggcc cgcatcactg cttggggccg taccggcgca tgaacttctc
     6961 cacgcggccg gccgtgtcca cgtactgcct gcgacccgtg tagaacgggt ggttggtcga
     7021 atcgacctcc gacgaatggc gatcgccctc gatggtcgag cgcgtcttga tctccgtacc
     7081 gtcggcaagc tgcaccgtca cgaagtggta gtcgggatgc aggtcctttt tcatggcttt
     7141 cgggaaggaa tggcgccgaa tcggcgtccg atgcaacaga gccgaataat atacggttcc
     7201 tcgggcaccc ggtgcaaccc gggctcagga cacgacccac gtgtcgccgg cgcgcagcag
     7261 gtcgtcgagc cggcccttgc ccgagcggtc cgtgatctcg gcgatctggg cgtgcatctg
     7321 gtcctcgtac gtgggccgct ccacccggta gaacacgccg aagggctgcg gcatgtcgtc
     7381 ccgccggaac aggcggccgg cgatcgacgc cagctcgaag ctggtctcgt cgtagaccag
     7441 gcagtcgtcc gccgaccagg gtccgtcctg aaggtccacg gagaccaggt gaaatccttc
     7501 caggcgcagt ccgcgcgtcc cgttggcgta gaccagcggc cggccgtgct cgatgaagag
     7561 cgtgcgctcg ggcttcgtgt ccttctcggt gtactggaag aag
//
LOCUS       DBNHQZ010000401         5219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_254564, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000401 DBNHQZ010000000
VERSION     DBNHQZ010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_254564"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..2234)
                     /locus_tag="AC16YU_10960"
     CDS             complement(15..2234)
                     /locus_tag="AC16YU_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR6065789.1"
                     /translation="MEGNFSNRVRDVISFSREEAIRLGHDYIGTEHLLLGIIREGEGI
                     AVKILKNLGSDLFKLKKAIEETVRSTGGTLTVGNIPLTKQAEKVLKITYLEAKLYKSD
                     VIGTEHLLLSLLRDDENLAAQILQQGFSVTYDAVRSELDSIVSGKASARGTSGGRSGG
                     SRPQGGQKERGKMEKSKTPVLDNFGRDLTRLAEEGKLDPVVGREREIERVAQVLSRRK
                     KNNPVLIGEPGVGKTAIAEGLAMRIVQRKVSRVLYDKRIVTLDLAALVAGTKYRGQFE
                     ERMKAVMNELEKSPDVILFIDELHTIVGAGGASGSLDASNMFKPALARGEIQCIGATT
                     LDEYRQHIEKDGALDRRFQKILVDPSSPEETIEILSNIKERYEEHHNVRYDDEALRLT
                     VRLSERYITDRFLPDKAIDVMDEAGARVHLSNIRVPADVVRLEEEIEGIRDEKNRVVK
                     SQKFEEAARLRDREKKLLEDLETAKKAWEEKAESEVHDVTVDDISQVVAMMTGIPVDK
                     ISEPETKKLLRMEEELQEAVVGQDEAIRKLSRSIRRTRAGLKDPKRPIGSFIFLGPTG
                     VGKTELAKKLTEYLFDSQDALIRVDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTEKV
                     RRKPYSVVLLDEIEKAHPDVFNILLQVLDDGVLTDGLGRKVDFRNAIVIMTSNIGARD
                     IRNLGKGIGFEQSDTEFDYQALKSTVQDALKRVFNPEFLNRIDDVIVFHPLEKKHIH"
     gene            complement(2344..2691)
                     /locus_tag="AC16YU_10965"
     CDS             complement(2344..2691)
                     /locus_tag="AC16YU_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR6065790.1"
                     /translation="MIATDSDTPVVLSERAAAEIRTIVENKELPDGFALRVGVRGGGC
                     SGMSYLLGFDRQREHDITFEIGGIRMLMDRRHGLYLMGTTIDYQDGLDARGFTFDNPN
                     AASTCGCGSSFTS"
     gene            complement(2781..5054)
                     /locus_tag="AC16YU_10970"
     CDS             complement(2781..5054)
                     /locus_tag="AC16YU_10970"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR6065791.1"
                     /translation="MRPACRIALACAAIVPSVAAAPPHARRPADAANPDSLRVSEVHH
                     APAEAALEFVELVNAGSGEIVLGGLTLADARDVPSTVPDPAGPLTPGGRLVLVRDAVA
                     FSGRWPGVPHLQVTPWPALNNSGDEVIVRIGGTVADRVAYLGAWGASGRSLERRDPSG
                     PSDHPVNWGASTDPSGATPGRVNSIHAPDRSPPAVLLSDRPAPDVVAVLVSEPVDVGD
                     AAFRRDGRDLALLLVAEDRTEALVRARPADPDGTLVVTGASDLFGNRLDRAETEVAHL
                     PRPGSLRISEVLSRPDRGRPGQVSFVEVVSSSERPQSLRGLVLQGAPDAYGIRRQAAV
                     PLLRQAVAPGERAVLFDPGSRADADSLFRAAFPSAASSALLAVPGWPFGSDPPEVRLA
                     AADGSRVASVRLRLTWHDPAIGPSTGRSLEHVAPLSGDSGPTAWTSSTHPSGATPGAS
                     NAAEALPRGRPPRAGDLRIGEILYEAAAGALPLQPEFVEIETASTDAVDPNGTFLVNE
                     AVGSVPDSVRIGFAPDALSRADFIVLAWIVSGSGAERVVGDLAAAFPSSSGRLPAHRI
                     RPAAGARSLRNGGATLVLRAADGTEIDRVEYAPSLHHPALRETAGVSLERVDRDGPSG
                     DPTNWTSTTHPEGATPGWRAGTPPRPPAAPGGLRIEPRVFTPSGDELSRHVAIHVLLH
                     EGPSVTDVHVFDLAGRAVRTLADGRLSNGPDVVYWNGLDDRGLPAPTGPYVVSVRSWS
                     AEGRPRGGRAVVVLARR"
BASE COUNT          772 a         1772 c         1843 g          832 t
ORIGIN      
        1 atgagcgaga tgatctagtg gatgtgcttc ttctcgagcg ggtggaacac gatcacgtcg
       61 tcgatccggt tcaggaactc cggattgaac acccgcttca gcgcgtcctg taccgtggac
      121 ttcagcgcct ggtagtcgaa ctcggtgtcc gactgctcga agccgatgcc cttgcccagg
      181 ttgcggatgt cccgcgcgcc gatgttcgac gtcatgatga cgatcgcgtt gcggaagtcc
      241 accttccggc cgaggccgtc cgtgaggact ccgtcatcga gcacctggag caggatgttg
      301 aagacgtccg ggtgcgcctt ctcgatctcg tcgagcagca ccaccgagta gggcttgcgg
      361 cgcaccttct cggtgagctg cccgccctcc tcgtagccga cgtagccggg aggggcgccc
      421 acgaggcggc tcacggagaa cttctccatg tactcgctca tgtccacccg gatgagcgcg
      481 tcctgcgagt cgaacaggta ctcggtgagc ttcttggcga gctcggtctt gcccacgccc
      541 gtcggcccga ggaagatgaa cgagccgatg gggcgcttcg ggtccttgag ccccgcccgg
      601 gtccggcgga tggaccggga gagcttccgg atggcctcgt cctgcccgac gaccgcctcc
      661 tggagttcct cctccatgcg gaggagcttc ttggtctcgg gttcggagat cttgtcgacc
      721 gggatgccgg tcatcatggc cacgacctgc gagatgtcgt ccaccgtaac gtcgtggacc
      781 tccgactccg ccttttcctc ccacgccttc ttggcggtct cgaggtcctc gagcagcttc
      841 ttttcgcggt cgcgaaggcg tgcggcctcc tcgaacttct ggctcttcac cacgcggttc
      901 ttctcatcgc ggatgccctc gatctcctcc tcgagccgga ccacgtcggc cggcacgcgg
      961 atgttcgaga ggtgcacgcg cgcgccggcc tcgtccatga cgtcgatggc cttgtccggc
     1021 aggaagcggt ccgtgatgta ccgctccgac aggcgaacgg tgagccggag cgcctcgtcg
     1081 tcgtagcgga cgttgtggtg ctcctcgtag cgctccttga tgttggagag gatctcgatg
     1141 gtctcctcgg ggctcgacgg atcgaccagg atcttctgga agcgccggtc gagcgccccg
     1201 tccttctcga tgtgctggcg gtactcgtcg agcgtggtgg cgccgatgca ctggatctcg
     1261 ccgcgcgcga gggccggctt gaacatgttc gacgcgtcca ggctgcccga tgcgccgccc
     1321 gcgcctacga tcgtgtggag ctcgtcgatg aagaggatga cgtcggggct cttctcgagc
     1381 tcgttcatca cggccttcat cctctcctcg aactggccgc ggtacttggt gcccgccacg
     1441 agcgccgcga ggtccagcgt aacgatccgc ttgtcgtaga gcacccggct caccttgcgc
     1501 tgcacgatgc gcatggccag gccctcggcg atcgccgtct tgcccacgcc gggctcgccg
     1561 ataagcaccg ggttgttctt cttgcgccgg ctgagcacct gcgcgacccg ctcgatctcc
     1621 cgttcgcgtc cgacgaccgg gtcgagcttt ccttcctcgg ccaggcgggt gaggtcgcgg
     1681 ccgaagttgt ccagaaccgg agtcttgctt ttttccatct tgccacgctc cttttggccg
     1741 ccctgtggac gggagccccc ggagcggccg ccggaggttc cacgagccga tgccttgccg
     1801 ctgacgatcg aatcgagttc ggagcggacc gcgtcgtacg tgaccgagaa gccctgctgc
     1861 aggatctggg cagccaggtt ctcgtcgtcg cggagcaggc tgagcagcag gtgctcggtc
     1921 ccgatgacgt cgctcttgta cagcttggcc tcgagatagg tgatcttgag caccttctcg
     1981 gcctgcttgg tgagcgggat gttgccgacc gtgagagtgc cgcccgtcga gcgaacggtc
     2041 tcctcgatcg ccttcttcaa cttgaaaaga tcggagccga ggttcttgag aatcttgacg
     2101 gcgattccct cgccctcgcg gatgatgccc agcagcaggt gctccgtccc gatgtaatcg
     2161 tgaccgagcc ggatcgcctc ctcccggctg aaagaaatga cgtcgcggac gcgattgctg
     2221 aagttccctt ccatgttcgg tcagttgtgc gcccgccggt agtccgcgcg gaaggccgac
     2281 ggccggtccg gaatttccgg ccggcgaggc gtcctccgca aggaagcgac gagtgcctgc
     2341 agctcaggac gtgaagctgc tgccgcagcc acaggtggaa gcggcgttgg gattgtcaaa
     2401 cgtgaaaccg cgcgcgtcga gtccgtcctg gtaatcgatg gtcgtcccca tcaggtacag
     2461 tccgtggcgc cggtccatga gcatgcggat gcccccgatc tcgaacgtga tgtcgtgctc
     2521 gcgctgccgg tcgaatccga gcaggtagct catccccgag cacccgccgc cccggacgcc
     2581 gacgcggagc gcgaagccgt ccgggagctc cttgttctcg acgatcgtgc ggatttccgc
     2641 ggccgcccgc tcgctcagca caacgggggt atcgctgtcg gtggcgatca tgattgattc
     2701 cggggtggtt gttggacggg acacttggcc tcgccgatcc aacgaagccc tgagggggca
     2761 gttccgaccc gaaaagggcc tcaccgccgc gcgagaacca ccacggcgcg gccgccgcgg
     2821 gggcgtcctt cggccgacca ggagcgaacc gacaccacgt acgggcccgt cggagcggga
     2881 aggccccggt cgtccaggcc gttccagtac acgacgtccg gcccgttcga gaggcgcccg
     2941 tccgcgagcg tgcggacggc ccggccggcg aggtcgaata cgtgcacgtc ggtcacggac
     3001 gggccctcgt gcagaagcac gtggatggcg acgtgccgcg agagctcgtc cccggagggc
     3061 gtgaacacgc ggggctcgat ccgcaggcca ccaggggcag cgggcggccg tggcggcgta
     3121 ccggcccgcc atcccggagt ggcgccttcc gggtgtgtgg tcgaagtcca gttggtggga
     3181 tccccggaag ggccgtcccg gtcgacgcgc tcgagcgaga cgccggcggt ttcgcggaga
     3241 gcggggtggt ggagcgacgg ggcgtactcg acgcggtcga tctccgtgcc gtcggccgcg
     3301 cgaaggacga gcgtggcccc gccgttccgg agcgaccgtg cgccggccgc cggacggatc
     3361 cggtgcgccg ggaggcgccc ggacgaggac ggaaaggcgg cggccaggtc gcccacgacg
     3421 cgttcggcgc cgctcccgga tacgatccag gcgagcacga tgaagtccgc gcgcgaaagg
     3481 gcgtccgggg cgaagccgat gcgcacggag tccggcacag acccgaccgc ctcgttcacg
     3541 aggaaagtcc cgttcggatc gaccgcatcc gtcgacgcgg tttcgatttc gacgaactcc
     3601 ggctgcaggg ggagggctcc cgcggcggcc tcgtacagga tttcgccgat cctcaggtcc
     3661 cccgcccggg gcggccgccc cctggggagc gcttcggccg cgttggacgc gccgggcgtc
     3721 gcgcccgagg ggtgggtgga gctggtccag gccgtcgggc cggagtcgcc ggacaggggg
     3781 gcgacgtgct cgagactgcg gcccgtggac gggccgatcg ccggatcgtg ccaggtgagc
     3841 cgcagcctca ccgaggcgac ccgggatccg tcggccgccg cgagacggac ctcgggcgga
     3901 tcggaaccga acggccatcc gggcacggcg aggagcgcgg acgatgcggc cgacgggaag
     3961 gctgcccgga acagggagtc cgcgtcggcc cgcgagccgg gatcgaagag cacggccctc
     4021 tcgcccggcg ccacggcttg ccggaggagg ggaacggccg cctgtcgtcg gatgccgtac
     4081 gcgtcgggcg cgccctggag cacgagaccg cggagcgact gcggccgctc cgacgaggac
     4141 acgacctcga cgaacgagac ctgccccggc ctgccccggt ccggccggct gaggacctcc
     4201 gagatgcgca gggatccggg gcggggcaga tgcgccacct cggtttccgc acggtcgagc
     4261 cggttgccga acaggtcgct cgccccggtc acgacgagcg tgccgtccgg atccgccggc
     4321 cgcgcccgga cgagcgcttc ggtgcggtcc tcggcgacca ggagcagggc gaggtcccgc
     4381 ccatccctcc ggaacgcggc gtctccgacg tccacgggct cgctgaccaa gacggcgacg
     4441 acgtccggcg ccggacggtc cgagagcagc acggccggag gcgagcgatc cggtgcgtgg
     4501 atcgagttca cccgccccgg cgtcgccccc gaggggtcgg tcgaggcgcc ccagttcacg
     4561 ggatggtccg agggtccgga cggatccctc ctttcgaggg accggccgga tgcgccccag
     4621 gctccgaggt acgctacccg gtccgccacc gtcccgccga tgcggactat gacctcgtcc
     4681 ccggagttgt tcagcgccgg ccacggggtc acctgcagat gagggacgcc gggccaccgg
     4741 ccggagaagg ccacggcatc gcggaccagg accaaccggc cgcccggcgt gaggggcccc
     4801 gcgggatcgg gcacggtgga ggggacgtcg cgcgcatcgg cgagcgtgag cccgccgagg
     4861 acaatctccc cggatccggc attcacgagt tccacgaact cgagtgccgc ctccgccggg
     4921 gcatggtgga cctccgaaac gcggagcgag tccgggttgg cagcatctgc agggcggcgc
     4981 gcatgcggcg gcgcggccgc caccgagggc acgatcgccg cgcaggcgag ggcgattcgg
     5041 cacgcgggac gcatcgctcg acgggtttac ccccttgaca cgcagaggcg ggcccccgta
     5101 cctacgaggc acttgccaag gacgaacatg gaaacggatc gcctggacga actggagcgc
     5161 cgcgtggccg ccctggaggc cgaactggga gcctggcgca gccgcacgcg ccagctcct
//
LOCUS       DBNHQZ010000402         3378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2827852, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000402 DBNHQZ010000000
VERSION     DBNHQZ010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2827852"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2015)
                     /locus_tag="AC16YU_10975"
     CDS             complement(<1..2015)
                     /locus_tag="AC16YU_10975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR6065792.1"
                     /translation="MKWRTTERVLAALVFAYALVLYVLTVAPATSFWDSGEFIAIANR
                     LQVSHPPGAPFYMLVGRLFSMFVPVGLVALSVNLVSVLASAATVLLAHLIIVRLVREW
                     QGAPSTWTTVDRIVALAGGAIGACTFAVTDSFWFNAVEAEVYALSMFFTAAVVWLILL
                     WREQAEHEEAAMRGGHHPFGLAANRYLVLIAYFFGLAIGVHLLNLLAIFFVALIVFFV
                     EFDREDWSTGKRFVGIVLAGLVSSVGFLVVYPGIIQFLPGVVGDSGSPVLVVTLILAA
                     VAVATWYTHKRRMQALNIVALCVVMVLIGYSTYALIFIRSASDPPIDENDPETVEAIV
                     SYLKREQYGATPILKGATYDNRTGQIDPAKESWLPRRYSPEPLHMRMYARYDSDSEFF
                     WKYQVGHMYLRYFLWNFMGRASDVQDAPAIVGLSEKETMQYVYQTPSERKSRNAYFGL
                     PLLLGLVGAAFHFLRDWRRAFAVLVLFLVTGIGIILYLNQTPMQPRERDYSYVASFFA
                     FSLWVGMGGAGVIQLASEALRSRFREGTAGLERTLVAIAVVIFAAVPLLMAVVNFDDH
                     DRSGRYVAGEYAYNMLMSVDENAILLTNGDNDTFPLWYMQEVEGVRQDVRVANLSLMN
                     TSWYVRQLKNQPSRTSEPLPISMPEDQIRDLTVIPWVPREITLPVNR"
     gene            complement(2056..2784)
                     /gene="rsmI"
                     /locus_tag="AC16YU_10980"
     CDS             complement(2056..2784)
                     /gene="rsmI"
                     /locus_tag="AC16YU_10980"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6065793.1"
                     /translation="MSAGTLYLVPTPIGNLEDLTFRAARILKEVDLVACEDTRTSAVL
                     LQHYGIAASRTSFHAHNEHGKAGRLADRIAAGESVALVSDAGTPGISDPGYLLVRACL
                     ERGIPVVALPGATAFVPALVASGLPSDRFVFEGFLPPKKGRRTRLAQIAAEERTVVLY
                     ESPHRIGRTLGELAAACGGERPAAVARELTKRFEEVRRGTLDELAAHFSETARARGEF
                     VVVIGAAKGPGAAAPGESNGPDPR"
     gene            complement(2781..>3378)
                     /locus_tag="AC16YU_10985"
     CDS             complement(2781..>3378)
                     /locus_tag="AC16YU_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6065794.1"
                     /translation="GLGTPSEWPPVAVEIASDLPGAAPEPVPGLDGGHPALAGLDSAG
                     HRQVFDVALQNARAGRSVVESARHVIQRATQRADQLRVEIHKKNSIAVACIIFMLIGA
                     PLGLSIRRGGLHLAALIAIGIFLFYWVTLVNGEKLADRGLLTPWVGMWAANIVGGIAA
                     AWLTLHVALDRRATPTRWSRLRARLFPAPDEANGDAEA"
BASE COUNT          575 a         1156 c         1185 g          462 t
ORIGIN      
        1 tcccggttga cgggcagcgt gatctcccgc ggcacccagg ggatgaccgt gaggtcgcgg
       61 atctggtcct ccggcatcga gatcggaagc ggctcggagg tgcgcgacgg ctggttcttg
      121 agctgccgca cgtaccagga cgtgttcatc agcgagaggt tcgccacgcg gacgtcctgg
      181 cggacgccct cgacctcctg catgtaccag agcggaaacg tgtcgttgtc cccgttggtc
      241 aggaggatcg cgttctcgtc cacgctcatg agcatgttgt atgcgtattc tccggccacg
      301 taccgcccgg agcggtcgtg gtcgtcgaaa ttcaccacgg ccatgagaag cggcacggcg
      361 gcgaagatca cgacggcgat cgcgacgagg gtccgctcga ggccggccgt accctcccgg
      421 aaccgggacc ggagcgcctc ggacgcgagc tggatcacgc ccgcgccgcc catgcccacc
      481 cacaggctga aggcgaagaa actggccacg taagagtagt cgcgctcgcg cggctgcatg
      541 ggggtctggt tcagatacag gatgatcccg atcccggtca cgagaaacag gaccagcacg
      601 gcgaacgccc gtcgccagtc acgaaggaag tggaaagccg cgcccacgag cccgagcaga
      661 aggggaaggc cgaagtaggc gttccgcgac ttgcgctccg acggggtctg gtagacgtac
      721 tgcatcgtct ccttctccga cagccccacg atggcgggcg cgtcctgcac gtcggacgcg
      781 cgccccatga agttccacag gaagtaccgc aggtacatgt gccccacctg gtacttccag
      841 aaaaactcgc tgtccgagtc gtaccgggcg tacatgcgca tgtgcagcgg ctcgggcgag
      901 taccggcgcg ggagccagga ttccttcgcg ggatcgatct gtcccgtgcg gttgtcgtag
      961 gtcgcgccct tgaggatcgg cgtggccccg tactgctcgc gcttcaggta ggagacgatc
     1021 gcctcgacgg tctccgggtc gttctcgtcg atgggcgggt ccgaggcgga gcggatgaag
     1081 atgagcgcgt acgtcgagta gccgatgagc accatcacga cgcacagggc cacgatgttg
     1141 agcgcctgca tgcgccgctt gtgcgtgtac caggtggcca ccgcgacggc cgcgaggatg
     1201 agcgtgacca cgagcaccgg gctgcccgag tcccccacga cgcccggcag gaactggatg
     1261 atgccggggt acacgaccag gaatccgacc gacgagacca gccccgcgag cacgatgccc
     1321 acgaaccgct ttcccgtgga ccagtcctcg cgatcgaact cgacgaagaa gacgatgagc
     1381 gccacgaaga agatggcgag caggttcagc aggtggacgc cgatcgcgag gccgaagaag
     1441 tacgcgataa gcaccaggta gcggttcgcc gcaaggccga acggatggtg cccgccccgc
     1501 atcgcggcct cctcgtgctc ggcctgctcc cgccacagga ggatcagcca gacgaccgcg
     1561 gccgtgaaaa acatggacag cgcgtagacc tcggcctcga cggcattgaa ccagaacgag
     1621 tccgtcaccg cgaacgtgca cgccccgatg gccccgcccg cgagcgccac gatccggtcc
     1681 acggtggtcc acgtcgacgg ggcgccctgc cactcgcgca cgagccgcac gatgatgagg
     1741 tgcgcgagca gcacggtcgc cgcgctcgca agcaccgaga ccaggttcac cgacagggcc
     1801 acgaggccca cgggcacgaa catcgagaag agccgcccca ccagcatgta gaacggcgcg
     1861 cccggcgggt gcgagacctg cagccggttg gcgatggcaa tgaactcgcc ggaatcccag
     1921 aacgacgtgg ccggtgcgac cgtgagcacg tagagcacga gcgcgtacgc gaagaccagc
     1981 gcggccagca cgcgctccgt ggttctccat ttcatgggaa atgcgccttg ggtcggatgg
     2041 gagtcggcac gcgtcctaac gcgggtccgg cccgttggat tcccccggtg cggccgcgcc
     2101 cgggcccttc gcggcaccga tcaccacgac gaattcgccc ctcgcgcgcg cggtctccga
     2161 aaagtgcgcg gccagctcgt cgagcgtgcc ccggcggacc tcctcgaacc gcttggtgag
     2221 ctcccgcgcc accgcggccg gccgctctcc gccgcacgcc gccgcgagct ccccgagcgt
     2281 ccggccgatg cggtgcggcg actcgtagag cacgacggtc cgctcctcgg cggcgatctg
     2341 cgcgagccgg gtccgccgac ccttcttcgg gggcaggaag ccctcgaaca cgaaccggtc
     2401 cgacgggagg cccgacgcca cgagcgccgg gacgaaggcc gtggcgcccg ggagcgccac
     2461 gacggggatc ccgcgttcca ggcacgcccg gaccaggagg tacccggggt cggagatgcc
     2521 cggcgtgccc gcgtccgaga ccagggccac gctctcgccg gcggcgatcc ggtccgccag
     2581 gcggccggcc ttgccgtgct cgttgtgggc gtggaagctg gtgcgcgacg cggcgatgcc
     2641 gtagtgctgc aggaggacgg ccgacgtgcg cgtgtcctcg caggccacga gatcgacctc
     2701 cttcaggatc cgggctgccc ggaaggtcag gtcctccagg ttgccgatgg gcgtcggtac
     2761 caggtacagc gtgcccgcgc tcatgcctcc gcgtctccgt tcgcctcgtc cggggcgggg
     2821 aacagccgcg cccgcagacg ggaccagcgc gtgggcgtcg cccggcggtc gagcgccacg
     2881 tgaagcgtca gccatgcggc ggcgatcccg ccgacgatgt tggcggccca catgcccacc
     2941 cagggggtga gcaggccgcg gtccgcgagc ttttcgccgt tgacgagcgt cacccagtag
     3001 aagaggaaga tcccgatcgc gatcagcgcg gcgaggtgca gaccgccgcg ccggatgctg
     3061 agtccgagcg gcgcgccgat cagcatgaag attatgcagg ccacggcgat cgagttcttc
     3121 ttgtggatct cgacgcgaag ctggtcggcc cgctgcgtgg cccgctggat gacgtgccgc
     3181 gcgctctcga cgaccgagcg gccggcccgg gcgttctgga gcgcgacatc gaagacctgg
     3241 cggtgcccgg cggagtcgag cccggcgagc gcgggatggc cgccatcgag cccgggcacg
     3301 ggttcgggag cggcgcccgg aaggtccgat gcgatctcca ccgctacggg gggccactcg
     3361 gacggcgtgc ccaggccc
//
LOCUS       DBNHQZ010000403         2722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2828002, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000403 DBNHQZ010000000
VERSION     DBNHQZ010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2828002"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="AC16YU_10990"
     CDS             <1..648
                     /locus_tag="AC16YU_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065795.1"
                     /translation="LGFSESFRMPASSPLEQNYLAYLFPGILLLTVLFTALFSTISII
                     EERRTGFLQAAVVAPVRRSALLAGTLSGGVTLAMAETLLFLAALPLLPMRPGWSGVLL
                     AFLVCALAATAFTAMGVAFAWRMSSTRGFHGVMNVLMLPLWLVSGAVFPVEGAHPVLR
                     IVMQLNPVTYVMNALRDALAHGTAAGIDLAVTAGFAAFLVAVGIRIIRNAPAPAS"
     gene            657..1727
                     /gene="rlmN"
                     /locus_tag="AC16YU_10995"
     CDS             657..1727
                     /gene="rlmN"
                     /locus_tag="AC16YU_10995"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR6065796.1"
                     /translation="MPQSVQDLSSLSRPELEAFATSLGEPAFRGRQLFRWIFGRLEAD
                     FDRMTDLPAAFRRRLSDVARIAVPNEAGRQTASDGTIKFLFALDSGRLVESVLIPDFE
                     EDGRANRLTVCVSSQVGCAMACSFCATGQMGFHQNLHAGEIYFQVALLNRVAVERFGR
                     AVTNVVYMGMGEPLQNYASVLQSVGLLTDPEALGMAARRITVSTVGLAARIRRLADDS
                     FAGNLAVSLHAPDDGKRSDIMPVNRGARTDLAALRDAIRYYARKTGRGVTYEYCMFRG
                     FNDTDQDARRLASVVGWAPSKVNLIMYNPVPGLGFERSDENRLNAFIRILVEHDVVVT
                     VRRSRGQDIDAACGQLATRESG"
     gene            1799..2002
                     /locus_tag="AC16YU_11000"
     CDS             1799..2002
                     /locus_tag="AC16YU_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065797.1"
                     /translation="MSKPRRLRLDLVQWLTGFLGAALVVAIVPQVARFLLRRVVGKFL
                     LETIAFAAVGVLLERGLSLIDRR"
     gene            complement(2033..>2722)
                     /locus_tag="AC16YU_11005"
     CDS             complement(2033..>2722)
                     /locus_tag="AC16YU_11005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6065798.1"
                     /translation="RVWLPLGVAAVALPLFVRQERRALDPVVRPGLFRSRQVALASVL
                     AAGAGLSESVFIFLPEFLVATFSVPVPTASFMLLPLMSAIAVTSLVAGRVLYRTGSRA
                     VIVTCQALLAVGLTAVAVAGDRLAVFYAGSVLIGAGLAGILGPALSYILLHEARIQER
                     TVSQGLITLFISVGQLIGGAAIGAVAASTGSGAEGYGSAFLVIAGVAGVVLLMAFGLK
                     SRAAERAGLAE"
BASE COUNT          394 a          985 c          914 g          429 t
ORIGIN      
        1 ctcgggttca gcgagtcgtt ccgcatgccc gcctcctcgc cgctggagca gaactacctg
       61 gcgtacctgt tccccggcat tctcctgctt acggtgcttt tcacggccct cttctcgacg
      121 atatcgatca tcgaggagcg gcggacgggc ttccttcagg cggcggtcgt cgcaccggtc
      181 cgtcgctccg ccctcctggc cggtacgctt tccggcgggg tgaccctggc gatggcggag
      241 accctgctgt tcctcgcggc gctcccgctg cttccgatgc gaccgggatg gtccggcgtg
      301 ctcctggcgt tcctcgtctg cgccctcgcc gccacggcat tcacggcgat gggggtggcg
      361 ttcgcatggc gcatgtcctc gacgcgcggc ttccacgggg tgatgaacgt actcatgctg
      421 ccgctctggc tcgtctcggg agccgtcttt cccgtcgagg gtgcacaccc cgtgctgcgc
      481 atcgtgatgc agctcaatcc ggtcacgtac gtcatgaacg ccctgcggga cgccctcgcg
      541 cacggcaccg cggcgggcat cgacctcgcg gtcacggccg gctttgccgc gttcctggtg
      601 gcggtgggca tccggatcat ccggaacgcg cccgccccgg cgtcgtgaca ccgaccatgc
      661 cgcaatcggt ccaggatctc tccagcctct cccgtcccga gctcgaggcc ttcgccacgt
      721 cgctcggcga gcccgccttc cgcggcaggc agctcttccg ctggattttc ggccggctcg
      781 aggccgactt cgaccggatg acggacctgc cggcggcctt ccgccggcgc ctgtccgacg
      841 tcgcacggat cgccgtgccg aacgaggcag gtcgccagac ggcatccgac ggcacgatca
      901 agttcctctt cgcgctggat tccggccgcc tcgtggagtc cgtcctcata ccggatttcg
      961 aggaagacgg ccgggcgaac cgcctgaccg tctgcgtctc gagccaggtc ggctgcgcga
     1021 tggcatgctc cttctgcgcg accgggcaga tgggcttcca tcagaacctg catgcgggag
     1081 agatctactt ccaggtcgcg ctcctgaacc gggtggccgt ggagcgattc ggtcgcgcgg
     1141 tgacgaacgt ggtctacatg ggcatgggcg agccgctcca gaactacgcg tccgtgctgc
     1201 agagcgtcgg actgctcacg gacccggaag ccctgggcat ggcggcccgt cggatcaccg
     1261 tgtccacggt gggactcgcg gcccgcatcc ggcgactggc cgacgacagc ttcgccggca
     1321 acctggccgt ttcgctgcac gcgccggacg acggcaagcg ctccgacatc atgccggtga
     1381 accgcggagc gcgcacggac ctggccgcac tgcgcgacgc gattcgctac tacgcgcgaa
     1441 agaccggacg gggcgtcacg tacgaatact gcatgttccg gggattcaac gacacggacc
     1501 aggacgcccg gcgcctggcc tcggtcgtcg gatgggctcc gagcaaggtc aacctgatca
     1561 tgtacaatcc cgtgccgggg ctcggcttcg agcggtcgga cgagaatcgg ctgaacgcgt
     1621 tcatccgcat cctcgtggag cacgacgtgg tcgtcaccgt gcggcggagc cgggggcagg
     1681 acatcgacgc cgcctgcggg cagctcgcca cgcgggagtc cggctgaacc ggggctgctg
     1741 cgacgattac cgatgcgggg cgccctatat tcggtccaaa gtcaacgcat ctcttgccgt
     1801 gtccaaaccg cgccgcctcc gcctggacct ggtgcagtgg ctcaccgggt ttctgggtgc
     1861 cgccctcgtc gtcgccatcg tcccccaggt cgcccgcttc ctgctccggc gtgtggtcgg
     1921 gaaattcctg ctcgagacca tcgcgttcgc ggccgtcggg gtcctgctcg agcgcggcct
     1981 ctcgctcatc gaccggcgct gaacgaaggg cgccgtcggg ccgtgacgcg gctcactccg
     2041 ccaggcctgc ccgttccgcg gcgcggctct tcaggccgaa tgccatgagg agcacgaccc
     2101 ccgccacgcc cgcgatgacg aggaacgcgc ttccgtagcc ctccgccccg gatccggtcg
     2161 acgcggccac cgccccgatc gccgccccgc cgatgagctg ccccacgctg atgaagagcg
     2221 tgatgagtcc ctgcgatacc gtgcgctcct ggatccgggc ctcgtgcagc aggatgtagc
     2281 tgagcgcggg gcccaggatg cccgccagtc ccgcgccgat caggaccgag ccggcgtaga
     2341 acacggcgag ccggtcccct gcgaccgcca ccgcggtgag cccgacggcg agcagcgcct
     2401 ggcaggtgac gatcaccgcc cgggacccgg tccggtacag cacgcgcccg gccacgagcg
     2461 aggtcacggc gatggccgac atgagcggca ggagcatgaa cgaggccgtc gggaccggaa
     2521 cggagaacgt ggccacgagg aactcgggca ggaagatgaa gaccgactcg ctgagccccg
     2581 ctcccgcggc gagtaccgag gccagggcca cctgccggct gcggaacagg cccggacgca
     2641 cgaccgggtc caacgcccgt cgttcctggc gcacgaacag gggcagcgcc acggcggcca
     2701 cgccgagcgg cagccagacg cg
//
LOCUS       DBNHQZ010000404         9570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_119594, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000404 DBNHQZ010000000
VERSION     DBNHQZ010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_119594"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1330)
                     /locus_tag="AC16YU_11010"
     CDS             complement(134..1330)
                     /locus_tag="AC16YU_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR6065799.1"
                     /translation="MPPSDALITEPTSLFAVLAGVLGLVFGLSRIRALEGFFRYLPPV
                     IWAYFLPMFLTTFGVTPSASVTYDWMTRYLLPFSLFLLMITVDLPAILRLGPRALAMM
                     VAGTAGIVIGGPVALAVFGPFLPDDAWMGFAALSGSWIGGTANLVAIKESIGAPDSIL
                     GPIIVVDTVVGYGWMGVLLFLSAFQDRFDRWVGADSRLIEQVNRRLLEIDTSRVPIGV
                     PDLTVILGITFVASVIAVWIGDDLLPPLGDPTIISGTTWAVLVVVTAGLALSFTPLRN
                     LERPGASRIGFLALYLLLTSIGARADLAAVVEAPLYLVAGVLWISIHIGILILVARII
                     RAPLFFVATGSMANIGGAASAPIVASVYLPAMAPVGLLMAVAGYILGIYAALGCAWLL
                     SLVASL"
     gene            complement(1343..2764)
                     /locus_tag="AC16YU_11015"
     CDS             complement(1343..2764)
                     /locus_tag="AC16YU_11015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6065800.1"
                     /translation="MTPRSLQVLLVAGEDASDRLAGLLGACSRPAMEVTRAASVDDAS
                     LIAGSRDLDVVVLDARIAPDLDRGVLRLPSVLLGVPVVLSVDESWTGPPPEGVADYLV
                     PDRDPAHVVERLLTLASQRHAMLRTLREAESQLRAIVEGISDGVMILGERDEVLYANP
                     AVESILEIPLVAILGAPSPIAVRDDSASVIEHDDGAGAARILRVSSIPILWERRQARL
                     VTFQDITAERAIQDSLRRARDEAERVAGLKTSFLANMSHELRMPLASMIGFAQIIHEG
                     SEHKEHREFAGSIIESGNRLLETITSILDLTRLDADAVSTETRPVSVVAIAREAVGML
                     SALASRKGLKLEVAESRHDDTVNTDPSILRRILANLIGNAIKFTDRGGVTVQVEADDA
                     FVRTHVTDTGKGISAEFIPRLFDEFTQESSGFGRSHEGSGLGLAITRRLCEVIGAKID
                     VESTQGEGSTFTLTIPHHGAWAN"
     gene            complement(2770..3906)
                     /locus_tag="AC16YU_11020"
     CDS             complement(2770..3906)
                     /locus_tag="AC16YU_11020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6065801.1"
                     /translation="MAASDLSRILSARHDALRSALREAALDALVVNPGPTLGYLTGLH
                     FHLSERPVLAFFPVDGAPVMVVPELESQKTDAAAFGLTVHTYPERPDLWQAAFNAGVA
                     AAGLGSSRLGVEPRWLRYLELGFLRSAAPGSRLGSAEETVAALRMHKDDAEIAAMQNA
                     TEIAQDALTSTLGEMAEGITERETAAVLIQHLLRYGSEGELPFQPIIAFGPNSANPHA
                     VPTDRALQPGDLVLFDWGANFEGYFSDLTRTFTFGDMDPELLVIADLVEAANAAGREA
                     SGPGVPAGAVDRAARAVIEAGGYGPRFIHRTGHGLGLEVHEDPYIRGDNELVLAPGMT
                     FTVEPGIYLTGRGGVRIEDDVVITTDGARSLSDLPRAVVPLPIG"
     gene            4054..5355
                     /locus_tag="AC16YU_11025"
     CDS             4054..5355
                     /locus_tag="AC16YU_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR6065802.1"
                     /translation="MTSTNPPPGTQALLVRIFEEAVREVRPDRLVADGSWVTDCARPI
                     GSYRRVVLVSIGKGAIPFALAVERTLGDRLHGGLAVVPHGYAENRPADLDLPGRVRVI
                     EAGHPVPDQAGVEASRAVLRLAGEAGEGDLVIACISGGGSALLPAPPADVPIASIRDL
                     TRGLLRSGAPIHDVNAVRRALSRIAGGGLARAAAPAEVVGLVLSDVPGDDLAVVAGGP
                     LAASPTTADDALRVLSARGVEAPPEIVRHLRAAARRNEAEVRASMRLVGSNADLLRAA
                     ALVAGRLGIPATVHRDQLLGEAREAGVRVVRAAVSPQESPGIHLWGGETTVRVTGQGT
                     GGRNQELALGAAIELHATQGDCRVLAAGSDGIDGATRAAGAVCDGRTVALGMERGLDA
                     GRLLDDNDSHRFFASADGLVVTGPTHTNVMDLVAVLRPGAA"
     gene            complement(5393..6400)
                     /locus_tag="AC16YU_11030"
     CDS             complement(5393..6400)
                     /locus_tag="AC16YU_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR6065803.1"
                     /translation="MRNRLLGILLVVLTVVGGLNLVQAVRAHFWSDGDASASSADFKA
                     RTASRSSDAVRDGVFLDRTFKVADGQLLEIAVQHADVTVETGETDEARVTVSVSASSE
                     DRAREIFERMNFEAAQDGDRVILRSTPRSEWNWNGRFRIEVRARIPRQFDLELASTHG
                     DVMLASIDGTVTLNTTHGDVDAERIAGPAVRLNSTHGEIHANALLADLVEVRTTHGDI
                     AVGTVRTTRLDAITTHSDIEIDELEGSGEVQTTHGDVTAGVLKHEGLSVRTTHGDVLL
                     VLPAGSGAAVNLRGEHVALPSGVDFEGVRDEERAEGTLNGGGPALEVSTTFGEVTLRA
                     D"
     gene            6594..9443
                     /gene="alaS"
                     /locus_tag="AC16YU_11035"
     CDS             6594..9443
                     /gene="alaS"
                     /locus_tag="AC16YU_11035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6065804.1"
                     /translation="MSRPQTAEETRADFLAFFREHGHEIVPSDSLVPAGDPTLLFTNA
                     GMNQFKDVFLGEGTRPYARAADTQKCLRVSGKHNDLEEVGYDTYHHTFFEMLGNWSFG
                     DYFKKEAIRWAWDLLVGRWGLDPSRLYATVHEGDPALGLDADDEAAGYWRSETGIDPD
                     HILYCSSADNFWMMGETGPCGPCSEVHVDLRPDEERARIDGRERVNSDDPRVIEIWNL
                     VFIQYNAQRDGTLQPLKAKHVDTGMGFERMVAVLQSRTSTYETDLFMPILDAVAALSP
                     RADVRGYDDLDAADEAGREKLRVALRVVADHVRTVAFAIADGVMPGNAGRGYVIRRIL
                     RRAVRFGYQTLELHEPFLYRIADTVIDLMKGQFPELERNRAYIERVVRAEEESFLETL
                     GTGLQFFDRVMPYLRALDDGESIDAVSARLAKDRPTLDLLEKAYVNAPDRAEIMRRYV
                     ESASRGAMPGEVAFLLHDTYGFPVDLTQLMAREVGFTVDMERYEALMQEQKDRARAAT
                     SFRVDQSSTDAWATVSSGHDSEFVGYERSRVEGSVVRAVRQVRLDDGVRNELILDVTP
                     FYAESGGQVGDTGTLLVGEEEIRVLDTQKRQGRIVHTVDRLPSDPSAPVTAEVDRGRL
                     VRIRKHHTVTHLMHAALREILGPHVAQKGSLVAPDHLRFDFSHFERVTPDELREVERR
                     VNEVIQRNIERREERGVPLKEALERGAMALFGEKYGDTVRVITFDPEYSVELCGGQHV
                     DATGEIGVFRFTSEGSVAAGIRRVEAVAGVDAVRHLHDELDELGRVRGQFRSLQRPSD
                     EEVAELLDRNRRLEKALEEARHVALASRLDAFLEDALMVGDVRLVIGQLDRLDMAALR
                     GLGETLRGQAGEGVVGILGATDPDGSKAYLVVTVSDDLVRRGLQAGRIVGELARTVEG
                     GGGGRPQLATAGGREPSKLPEALAEAPALIGSMLS"
BASE COUNT         1505 a         3234 c         3437 g         1394 t
ORIGIN      
        1 atcgacctga cggatcccgg cttctgggcg cgcggcctgg aggccatcgg tgagctcgtg
       61 gccagggccg aggccctggc ggaagagtca gggggctaga cccgttcaca cgtcccggaa
      121 cgtgtgcccg gccctagagc gaggcgacca gcgagagcag ccacgcacag ccgagcgccg
      181 cgtagatgcc caggatgtac ccggccacgg ccatgagcag gcccaccggg gccatggccg
      241 gcagatagac cgacgccacg atcggggcgg acgcggcgcc gccgatgttg gccatgctgc
      301 ccgtcgcgac gaagaaaagc ggggcacgga tgatccgcgc gaccagaatg aggatgccga
      361 tgtggatgga gatccacagg acacccgcga ccaggtagag cggggcctcc acgacggcgg
      421 cgaggtcggc cctcgccccg atcgaggtga ggagcaggta cagggccagg aagccgatgc
      481 gcgaggcgcc cggccgctcc aggttgcgga gcggggtgaa ggacagggca aggccggccg
      541 tcacgaccac gaggaccgcc cacgtggtgc ccgaaatgat cgtgggatca ccgaggggcg
      601 ggagcaggtc gtccccgatc catacggcga tcacggacgc gacgaacgtg attccgagaa
      661 tgaccgtgag atccggaacg ccgatcggca cgcggctcgt gtcgatctcg agcaggcgcc
      721 ggttcacctg ttcgatcagg cgcgagtcgg cgccgaccca ccggtcgaag cggtcctgga
      781 acgcggagag aaacagcagg acgcccatcc agccgtagcc gaccacggtg tcgaccacga
      841 tgatcggccc gaggatggaa tccggcgctc cgatcgactc cttgatcgcc acgagattgg
      901 ccgtaccccc gatccagctt ccggacaggg ccgcgaatcc catccaggca tcgtcgggca
      961 ggaagggccc gaacacggcg agcgccacgg gtcccccgat cacgatgccc gccgtgccgg
     1021 ccaccatcat cgccagggca cggggaccca ggcgcaggat tgccggaagg tcgaccgtga
     1081 tcatgaggag gaagagcgag aacggcagca ggtaccgggt catccagtcg tacgtcacac
     1141 tggccgaggg cgtgacgccg aaggtcgtca ggaacatggg caggaagtac gcccagatga
     1201 ccggcggcag gtaccggaag aagccctcga gcgcccggat ccggctcagg ccgaacacca
     1261 ggccgagcac gcccgcgagc accgcgaaca gggaggtggg ctcggtgatc agggcgtcgg
     1321 atggaggcat tcgctggtcg gttcagttgg cccaggcgcc gtgatgcggg atcgtcaggg
     1381 tgaaggtcga gccctctccc tgcgtgctct cgacgtcaat tttggcgccg atgacctcgc
     1441 acaggcggcg cgtgatcgcc aggcccaggc ccgagccctc gtgactccgc ccgaacccgc
     1501 tcgactcctg ggtgaactcg tcgaagagtc gggggatgaa ctcggcgctg atgccctttc
     1561 ccgtgtcggt aacgtgggtc cgcacgaagg catcgtcggc ctccacctgc acggtcaccc
     1621 cgccgcggtc cgtgaacttg atcgcgttgc cgatcaggtt ggccaggatc cggcgcagga
     1681 tggacgggtc ggtgttcacc gtgtcgtcgt ggcgcgactc cgcgacctcg agtttcagac
     1741 ccttgcgcga ggccagggcc gacagcatgc ccacggcctc gcgggcgatg gcgaccacgg
     1801 agacgggccg agtttccgtg ctcaccgcat ccgcgtcgag tcgcgtgagg tccaggatgg
     1861 aggtgatggt ctcgagcagg cgattgccgc tctcgatgat gctgccggcg aactcgcggt
     1921 gttccttgtg ctcgcttcct tcgtggatga tctgcgcgaa cccgatcatg ctggcgagcg
     1981 gcatgcgaag ttcgtggctc atgttggcca ggaacgaggt cttcaggccg gccacgcgct
     2041 cggcctcgtc gcgcgcccgc cgcaggctgt cctggatggc ccgctcggcg gtgatgtcct
     2101 ggaacgtgac gagccgcgcc tggcgacgct cccagaggat cgggatggag gacacccgga
     2161 ggatgcgggc ggccccggcc ccgtcgtcgt gttcgatgac cgacgcggaa tcgtcgcgga
     2221 cggctatcgg ggagggggcc ccgagaatcg ccaccagggg aatctcgagg atcgattcga
     2281 ccgcgggatt ggcgtagagc acctcgtcgc gctcccccag gatcatcacg ccgtccgaaa
     2341 tgccttccac gatcgcccga agctgggatt ccgcttcccg cagcgttcgc agcatggcgt
     2401 gacgctggct cgccagcgtg agcagccgct cgaccacgtg tgcggggtcc cggtccggga
     2461 ccaggtagtc ggccacccct tcgggaggcg gaccggtcca cgactcgtcc acggagagca
     2521 ccaccgggac gccgagaagg acggacggaa gccgcagcac gccccgatcg aggtcgggcg
     2581 cgatccgtgc gtcaagcacg accacgtcca ggtcgcggct gccggcgatg agcgaggcgt
     2641 cgtcgaccga cgccgcgcgg gtcacctcca tggcggggcg cgagcaggca cccagaagcc
     2701 cggcgagcct gtccgaggcg tcctccccgg cgacaagcag gacctggagc gatcgcggag
     2761 tcatggctgt cagccgatgg gcagcggaac cacggcgcgc ggcaggtcgc tcagggaccg
     2821 cgcaccgtcc gtggtgatga ccacgtcatc ctcgatccgc actccgcccc tccccgtgag
     2881 atagatcccg ggctccacgg tgaacgtcat gcccggagcc agcacgagct cgttgtcgcc
     2941 ccggatgtac ggatcctcgt gcacctcgag cccgagcccg tgacccgtgc gatggatgaa
     3001 gcgggggccg tatcctccgg cctcgatcac ggcgcgcgcc gcccggtcca cggcgccggc
     3061 ggggactccc ggtccggagg cctcgcggcc ggccgcgtta gccgcctcga cgagatcggc
     3121 gatgacgagc agctccgggt ccatgtcgcc gaacgtgaac gtgcgggtga gatcagagaa
     3181 gtagccctcg aaattggctc cccagtcgaa gagaaccagg tcgccgggct gcagcgcccg
     3241 gtcggtgggc acggcatgcg ggttggccga attggggccg aaggcgatga tcggctggaa
     3301 cggcagctca ccctcggaac cgtaccgcag gagatgctgg atgagcacgg cggccgtctc
     3361 gcgctccgtg attccctcgg ccatctcgcc gagcgtggat gtcagcgcgt cctgcgcgat
     3421 ctcggtggca ttctgcatcg ccgcgatctc cgcgtcatcc ttgtgcatcc gcagggcggc
     3481 gaccgtttcc tcggccgatc cgagcctgct cccgggcgct gcactgcgga ggaaccccag
     3541 ctccaggtac cggagccagc gcggctcgac gccgagccgg gacgagccca ggccggccgc
     3601 ggccacgccg gcgttgaacg ccgcctgcca caggtcgggc cgctcggggt aggtatggac
     3661 cgtgaggccg aacgcggcgg catccgtctt ctggctctcg agctccggca cgaccatgac
     3721 cggggcaccg tcgacgggga agaaggccag cacgggccgc tccgagaggt ggaagtgcag
     3781 gccggtgaga tacccgagcg tgggtccggg attgacgacc agcgcatcga gcgccgcctc
     3841 gcgaagggcc gagcgcaggg catcgtgtcg ggccgacagg atgcgggaca agtcggaagc
     3901 tgccatgggg atggattcgg agttcgggag aatcggcggc caagataaac gaccggggga
     3961 acgcttgccc ctcaaatccg gcgcggttgt cattcggatc gcacggttcg aacttccggc
     4021 tggtgccccg caagcctgag ccggaatccg atcgtgacgt cgacgaatcc tccaccggga
     4081 acgcaggcgc tgctcgtccg gatattcgag gaggccgtcc gggaggtccg ccccgaccgg
     4141 ctcgtcgccg acggatcatg ggtgacggac tgcgcgcggc cgatcgggtc ctaccgccgc
     4201 gtggtcctgg tgtcgatcgg caagggggcg attcccttcg cgctggccgt ggagcgcacg
     4261 ctgggcgacc ggctgcacgg cgggctggcg gtcgtgcctc acggctacgc ggaaaaccgt
     4321 ccggcggacc tggacctgcc cggccgcgtt cgcgtgatcg aggcgggcca tcccgtgccg
     4381 gaccaggccg gggtcgaggc ctcgcgggcg gtgctccggc tcgccggcga ggcgggggag
     4441 ggcgacctcg tgatcgcatg catatcgggc ggaggatccg cccttctccc ggcgccgccc
     4501 gcggacgtcc cgatcgcatc gatccgcgac ctgacgcgcg gccttctgcg ttccggcgcg
     4561 cccatccacg acgtcaacgc cgtgcgccgc gcgctctcgc gcatcgcggg cgggggactg
     4621 gcccgggcgg cggccccggc agaagtggtg ggcctcgttc tctcggacgt tcccggcgac
     4681 gacctcgcgg tcgttgccgg gggaccgctc gccgcgtcgc ccacgacggc cgacgatgcc
     4741 ctgcgcgtgc tctcggcgcg cggcgtggaa gccccgccgg aaatcgtccg ccacctgcgg
     4801 gcggccgcac gtcggaacga ggcggaggtc cgggcgtcca tgcgcctcgt gggctccaac
     4861 gccgacctgc tgcgcgcagc ggcgctcgtc gccgggcggc tggggatccc ggcgacggtg
     4921 caccgggatc agctgctggg tgaagcgcgc gaagccggcg tgcgggtcgt ccgtgccgca
     4981 gtctccccgc aggagtcccc cggcatccac ttgtggggtg gggagacgac cgttcgggtg
     5041 accggtcagg gcaccggggg gcgaaaccag gagttggccc tcggggcggc gatcgagctc
     5101 cacgccacgc agggcgattg ccgggtgctc gccgccggaa gcgacgggat cgacggcgcg
     5161 acccgggcgg ccggcgccgt gtgcgacgga cgcacggtcg ccctcgggat ggagcgcggc
     5221 ctcgacgcgg gccgactgct cgacgacaat gactcgcacc ggttcttcgc ctcggcggac
     5281 ggactcgtcg tcacggggcc gacccacacg aacgtgatgg acctcgtcgc ggtactccgc
     5341 ccgggtgccg cctgacggcg cccgtcggct cgggcggtgg cggattccgg gctcagtccg
     5401 cgcggagcgt gacctcgccg aacgtcgtgg agacctcgag cgcagggccg cctccgttca
     5461 gcgtgccctc ggcccgctct tcgtcccgga cgccttcgaa atcgaccccg gaaggcagcg
     5521 cgacgtgctc gcccctaagg ttcacggcgg cgccgctccc tgccggcaga accagcagca
     5581 cgtcaccatg cgtggtccgg acgctcagcc cctcgtgctt gaggacaccg gctgtcacgt
     5641 ctccgtgcgt cgtctggacc tcccctgagc cctcgagctc gtcgatctcg atgtcggagt
     5701 gcgtggtgat ggcgtccagg cgtgtcgtgc ggaccgtgcc cacggcgatg tcgccgtgcg
     5761 tggtccgaac ctcgacgagg tccgcgagga gcgcattcgc atggatctcg ccgtgcgtgc
     5821 tgttgagccg gacggcggga cccgcgatgc gctcggcatc cacgtccccg tgggtggtgt
     5881 tgagcgtgac cgtgccgtcg atcgatgcga gcatcacgtc accgtgcgtg ctggccagct
     5941 cgaggtcgaa ctgacgcggg atccgggccc gcacctcgat gcggaaccgg ccgttccagt
     6001 tccactcgct gcggggcgtc gaacggagga tgacgcggtc gccgtcctgg gcggcctcga
     6061 agttcatccg ctcgaaaatc tcgcgcgcac ggtcttccga agaggcgctc acggaaaccg
     6121 tgacgcgagc ctcgtccgtc tccccggtct cgaccgtgac gtccgcgtgc tgcaccgcga
     6181 tctcgagaag ctggccatcg gcgaccttga acgtccgatc gaggaagacg ccgtcccgga
     6241 cggcatccga ggacctggat gcggtccggg ccttgaagtc cgccgacgac gcggacgcat
     6301 ccccgtcgga ccagaaatgt gcacgtacgg cctggaccag gttcagtccg ccgactaccg
     6361 tgagtacgac gagcaggatt ccgagaagac ggttacgcat ggcggatcgg tcctccttgg
     6421 gcgggtgact ttcaccggta cggagaggcg ccggccgtgt tgcgctgcgc ccgcaatcgg
     6481 ggaccggggt ctccggacga ccgtttccgt ttccgtatat tcgctcggcc gaaccaggcc
     6541 ggtccccgtc cgatgtccgg atggggcctt tttcgtatgc gccgaagcga tccatgagcc
     6601 gaccgcagac cgccgaagag accagggcgg atttcctcgc gtttttccgg gagcacgggc
     6661 atgaaatcgt gcccagcgac tcgctcgttc cggcgggcga tccgacgctg ctcttcacga
     6721 acgcggggat gaaccagttc aaggacgtgt tcctgggcga gggcacgcgt ccttatgccc
     6781 gggcggccga cacgcagaaa tgcctgcggg tttcgggcaa gcacaacgac ctcgaggaag
     6841 tgggctacga cacgtaccac cacacgttct tcgagatgct gggcaactgg agcttcggcg
     6901 attacttcaa gaaggaagcc atccgctggg cgtgggacct gctcgtcggg cgatggggcc
     6961 tggacccgtc gcgcctgtac gccacggtgc acgaggggga cccggcgctc ggtctcgacg
     7021 ccgacgacga ggcggccggg tactggcgga gcgagacggg catcgatccc gaccacatcc
     7081 tgtactgctc gtccgcggac aatttctgga tgatgggcga gacgggcccc tgcggcccct
     7141 gctccgaggt gcacgtggac ctgcgccccg acgaggagcg ggcgaggatc gacggccgcg
     7201 agcgggtcaa ttcggacgat ccccgggtga tcgagatctg gaacctggtg ttcatccagt
     7261 acaacgcgca gcgggacggc accctgcagc cgctcaaggc caagcacgtg gacacgggca
     7321 tgggcttcga gcggatggtg gccgtgctcc agtcccggac gagcacgtac gagaccgacc
     7381 tcttcatgcc catcctcgac gcggtggcgg cgctctcgcc ccgcgccgat gtccgcggct
     7441 atgacgacct ggacgccgcg gacgaggcag ggcgcgagaa gctgcgcgtg gcgctccgcg
     7501 tcgtcgcgga ccacgtccgc acggtggcat tcgcgatcgc ggacggggtg atgccgggca
     7561 atgccggccg cgggtacgtg atccgccgca ttctgcggcg tgcggtccgc ttcggttacc
     7621 agaccctgga gctccacgag cccttcctgt accggatcgc ggacaccgtc atcgacctga
     7681 tgaagggcca gttcccggag ctggagcgta accgggccta catcgagcgc gtggtccgtg
     7741 ccgaggagga gagcttcctg gagaccctgg gcaccggcct gcagttcttc gaccgggtga
     7801 tgccgtacct gcgcgccctg gacgacggcg agtcgatcga tgccgtctcc gcacgcctcg
     7861 cgaaggaccg accgacgctc gacctgctcg agaaggcgta cgtgaacgcg ccggaccgtg
     7921 ccgagatcat gcggcgttac gtcgaatccg cctcccgcgg ggcaatgccg ggcgaggtcg
     7981 ccttcctgct gcacgacacg tacggattcc cggtcgacct gacccagctc atggcccggg
     8041 aggtcggctt cacggtggac atggagcgct acgaggccct catgcaggag cagaaggacc
     8101 gcgcccgcgc ggcgaccagc ttccgcgtgg accagagctc cacggatgcc tgggccacgg
     8161 tttcgtcggg ccacgactcc gagttcgtcg gctacgagcg cagccgggtg gagggctcgg
     8221 tcgtccgcgc cgttcggcag gtccggctcg acgacggggt gcgcaacgag ctaatcctgg
     8281 acgtcacccc cttctatgcc gagtcgggcg gccaagtggg cgacacgggc acgctcctgg
     8341 tgggcgagga ggagatccgc gtcctggata cgcagaaacg gcagggcaga atcgtccaca
     8401 cggtcgaccg gctcccgtcg gacccgtccg cgccggtcac ggccgaagtg gaccgcggcc
     8461 gcctggtccg catccggaag caccacaccg tgacccacct gatgcacgcg gcgctccggg
     8521 agattctcgg gccccacgtc gcacagaagg gatcgctcgt tgccccggac catctccggt
     8581 tcgacttcag ccacttcgag cgcgtgacac ccgacgaact gcgcgaggtc gagcggcggg
     8641 tgaacgaggt catccagagg aacatcgagc ggcgcgagga gcgcggggtg cctctcaagg
     8701 aggccctgga aaggggcgcg atggcgcttt tcggcgagaa gtacggcgac acggtacgcg
     8761 tcatcacgtt cgatccggag tattcggtcg agctctgcgg cggccagcac gtggacgcca
     8821 cgggcgagat cggcgtcttc cgcttcacct cggagggctc cgtggccgcc ggaatccgcc
     8881 gcgtcgaggc ggtggccggt gtcgacgccg tccggcacct gcacgatgag ctcgacgaac
     8941 tcgggcgggt gcgcgggcag ttccgctcgc tgcagcgccc ctcggacgag gaggtggccg
     9001 agctcctgga ccggaaccgc cgcctcgaaa aggccctcga ggaggcgcgc cacgtggcgc
     9061 tcgcgtcgcg gctcgacgcg ttcctggaag atgccctgat ggtcggcgac gtgcgcctgg
     9121 tcattggcca gctggaccgg ctggacatgg cggccctgcg cgggctcggg gagacccttc
     9181 ggggacaggc cggggagggt gtcgtgggaa tcctcggggc caccgatccg gacggctcga
     9241 aggcctacct ggtggtgacc gtctcggacg acctggtgcg gcgcggccta caggccgggc
     9301 gcatcgtcgg cgagctggcc cgcacggtcg aaggcggcgg cggcggccgc ccccagctcg
     9361 cgaccgccgg cggacgcgag ccctcgaagc tccccgaagc cctcgccgaa gcgcccgccc
     9421 tgatcggctc gatgctgtcc tgacgcggcc tccttcccgg ccgagtggac gtcgaaaacc
     9481 gcgggatccg aagccccgca ttgacgttgg tgcgcccgga gggatgccgg agtggttgaa
     9541 cggggcggtc tcgaaaaccg ttgtaccctt
//
LOCUS       DBNHQZ010000405         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2423632, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000405 DBNHQZ010000000
VERSION     DBNHQZ010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2423632"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="AC16YU_11040"
     CDS             <1..586
                     /locus_tag="AC16YU_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6065805.1"
                     /translation="AIRAEYPSAVLPIVLLTARSDTATLVQGTAAGADDVLVKPFDAE
                     ALAFRLENLIEGRRMLARSDTVGPTEIQVESSEKAFVERVREIVEEHLADSNFGVDWL
                     ADEVGKSPRQLQRRLRAATRLSAAGFIRMMRLGRARQLLAGGATNVSETAYAVGFHDV
                     DHFTRIFRQTFGKLPSEYAKQAASGEPPEPAPTV"
     gene            767..1105
                     /locus_tag="AC16YU_11045"
     CDS             767..1105
                     /locus_tag="AC16YU_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6065806.1"
                     /translation="MNIYVGNLPWRTTDDDLRDMFEPYGQVSSASVIRDRETDRSRGF
                     GFVEMPNSDEAQSAIAALNETDMGGRPLVVNEARPRQERRPGGGGGGGGGFRGGSRGG
                     GGFGGNRDRY"
     gene            1211..2455
                     /locus_tag="AC16YU_11050"
     CDS             1211..2455
                     /locus_tag="AC16YU_11050"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR6065807.1"
                     /translation="MQKLIAWFRETDIDWVTFPFIVGYHVVLLVALPLYLAYRTPGVD
                     LLLASLLLLTLSLLSITMGYHRLFSHRTYEAAGIVEWFLVFFGTATIQGSVVKWSHDH
                     RLHHKHVDKEGDPYGTPKGFWHSHVLWMFKKPAPLRENLVADLLRNPVLRSQHLHYGI
                     WVIVVNVVLTVGIGLAVGDLVGAFVIAFLLRLFLGHHSTWFINSIAHIWGSQPYSKEH
                     SAVNNFIIALLTYGEGYHNYHHTFAGDYRNGVKWYHFDPSKHAIWLLSKVGLTRNLRR
                     VSDLTIQRRLVVADRSLLLEHLQAVSANVSGLRQAGQDAMDEWRRRGHENAEHLVALA
                     HEQVERLRARVEEASHAFNRRVAEVKEATDAYKAAKSASTREEIRLARARLMALREEL
                     RGEWRAWQRLCDDVLDLRPMPA"
     gene            complement(2468..2599)
                     /locus_tag="AC16YU_11055"
     CDS             complement(2468..2599)
                     /locus_tag="AC16YU_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065808.1"
                     /translation="MQSDQLYKVALATVCSIAAILAIGLLVRGLLMGATALVDLVSG"
     gene            complement(2682..>3062)
                     /locus_tag="AC16YU_11060"
     CDS             complement(2682..>3062)
                     /locus_tag="AC16YU_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR6065809.1"
                     /translation="YIGGTPWSNPEEWRYRSALTHAANVTTPTLILHGIADPTDTEPQ
                     SMSFFAALKDLGKEVRYIRFPRELHGFREPRHQRMRDVEEIRWIHEHVTGSAWTPWPA
                     PKTGKPESGDEAGEPESGDESGSG"
BASE COUNT          509 a         1040 c         1010 g          503 t
ORIGIN      
        1 ggcgatccgc gccgagtacc cctccgcggt cctccccatc gtgcttctca ccgcccggag
       61 cgacacggcc accctcgtac agggcacggc ggccggcgcg gacgacgtcc tggtgaagcc
      121 gttcgacgcc gaggccctgg cgttccgcct cgaaaacctg atcgagggac gccggatgct
      181 cgcccggtcg gacacggtcg gtcccaccga aatccaggtc gagtcgtccg aaaaggcctt
      241 cgtggagcgc gtgcgcgaga tcgtggagga acacctggcc gactcgaact tcggcgtgga
      301 ctggctcgcg gacgaggtcg gcaagagccc gcgccagctg cagcgccgcc tccgggccgc
      361 cacgcgcctc tcggcggccg gcttcatccg catgatgcgc ctgggccggg ccaggcagct
      421 gctggccggc ggcgcgacca acgtctcgga gacggcctac gcggtcggat tccacgacgt
      481 ggaccatttc acgcggatct tccgtcagac tttcggcaag cttccgtccg aatacgcgaa
      541 gcaggcggcg agcggtgagc ccccggagcc ggccccgacc gtgtgaattc aagattattt
      601 tcacgaacac ggaacctgcg gatcttttgc cgcgcagcgc ttgttggggc gatcggtttt
      661 ggtgatcacg cccgcagtcg gaaactcagt cgaattagcc ctcgatcgtg caaccggaca
      721 ggtatgcgcc ggtggggtcg ccttggtttg aacggactct cagaacatga acatctacgt
      781 aggaaacctc ccctggcgca cgacggacga cgatctgcgc gacatgtttg aaccctatgg
      841 gcaagtgtcg agcgcatcgg tgatccggga ccgcgagacc gaccgctcgc gcggattcgg
      901 cttcgtcgaa atgcccaaca gcgacgaggc acagagtgcc atcgccgccc tgaacgaaac
      961 ggacatgggc ggacgcccgc tggtcgtgaa cgaggctcgt ccccgtcagg agcggcgccc
     1021 cggcggcgga ggcggcggcg gaggcgggtt ccgtggcgga tcccggggcg gcggcggctt
     1081 cggcggcaac cgcgatcgct actaggcgac ccggccggcc ccctcggccg gcagatcgga
     1141 tctgattcct gcgaaacccc ggtcccgccg gggtttcgta ttatgacccg ttcccaagct
     1201 accccggatc atgcagaagc tcatcgcctg gttccgtgag accgacatcg actgggtcac
     1261 cttcccgttc atcgtcggct accacgtcgt cctcctcgtc gccctgccgc tgtacctcgc
     1321 gtaccgcacc ccgggcgtgg acctgctcct ggcctccctc ctgctgctga cgctctcgct
     1381 cctgagcatc acgatgggat accaccggct gttctcgcac cgcacgtacg aggccgccgg
     1441 cattgtcgag tggttcctgg tgtttttcgg gacggccacg atccagggca gcgtggtcaa
     1501 gtggtcgcac gaccaccggc tgcaccacaa gcacgtggac aaggagggcg atccctacgg
     1561 gacgccgaag ggcttctggc attcgcacgt cctgtggatg ttcaagaagc cggcccccct
     1621 gcgcgagaac ctggtggccg acctgctgcg gaatccggtg ctgcggtcgc agcacctcca
     1681 ctacggcatc tgggtgatcg tcgtgaacgt cgtgctcacc gtgggcatcg gcctcgccgt
     1741 cggcgacctc gtcggggcct tcgtgatcgc gttcctgctg cggctcttcc tggggcacca
     1801 ctcgacgtgg ttcatcaact cgatcgccca tatctgggga tcgcagccct actcgaagga
     1861 gcactcggcg gtcaacaact tcatcatagc gctgctcacc tacggcgagg gctatcacaa
     1921 ctaccaccac acgttcgccg gcgactaccg caacggggtc aagtggtacc acttcgaccc
     1981 gtccaagcac gcgatctggc tcctgtcgaa ggtcggcctc acccgcaacc tccgtcgggt
     2041 gagcgacctg acgatccagc ggcggctggt cgtggcggac cggtcgctgc tcctcgagca
     2101 cctgcaggcg gtgtcggcga acgtgtccgg cctcaggcag gccggacagg acgccatgga
     2161 cgaatggcgc cggcgcggcc acgagaacgc ggagcacctg gtggcgctcg cccacgagca
     2221 ggtcgaacgc ctccgggccc gggtcgagga ggccagtcac gccttcaacc ggcgggtggc
     2281 cgaagtcaag gaggctaccg acgcctacaa ggccgcaaag tcggcctcga cacgcgagga
     2341 aatcaggctt gcgcgcgcgc ggctcatggc gctccgcgag gagctacgtg gcgagtggcg
     2401 ggcatggcag cgcctgtgcg acgacgtgct agacctgcgc ccgatgcccg cctgaggagt
     2461 tccaggttca gccggacacg aggtcgacaa gtgccgtcgc acccattaag agtccgcgca
     2521 ccaggagacc gatagccagg atcgccgcga tggaacagac cgttgcgagg gccaccttgt
     2581 agagttgatc cgactgcatg ggggtgtagc ggcagatgcg atccctgatt acgggaaaat
     2641 cgtgaagatc gcaagaaggc cgcgcgggaa accggaatcg ttcagcccga gccggactcg
     2701 tccccggact ccggctcccc ggcctcgtct ccggattccg gcttcccggt tttcggcgcg
     2761 ggccagggcg tccaggcgga cccggtaacg tgctcgtgga tccagcggat ctcctccacg
     2821 tcgcgcatcc tctggtggcg cggctcgcgg aacccgtgca gctcgcgcgg gaaccggatg
     2881 tagcgcacct ccttgcccag gtccttgagg gccgcgaaga aggacatcga ctgcggctcg
     2941 gtgtccgtcg gatccgcgat gccgtgcagg atgagcgtcg gggtcgtcac gttcgccgcg
     3001 tgcgtgagcg ccgaccggta gcgccactcc tccgggttgg accacggagt gccgccgatg
     3061 ta
//
LOCUS       DBNHQZ010000406         2047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2286398, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000406 DBNHQZ010000000
VERSION     DBNHQZ010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2286398"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16YU_11065"
     CDS             complement(<1..208)
                     /locus_tag="AC16YU_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6065810.1"
                     /translation="MDVERPMMGTLFRIVACAPDSGTGAEAADAAFARIREMEAALGD
                     YDPEGEVSRIARTYEPGVPVEVSAD"
     gene            complement(300..1673)
                     /locus_tag="AC16YU_11070"
     CDS             complement(300..1673)
                     /locus_tag="AC16YU_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065811.1"
                     /translation="MDTRKPGSGMSRRDFVRSTAAVVGSAAALRNPLKASAFFASDEV
                     IRVGLVGCGGRGTGAAAQALTAHPSVRLVAMADAFADRIEECHRNLTDPAATDYTGFD
                     TASIRDRVDVPPERRFTGFDAYEKVIPLADVVILTTPPGFRPLHFEAAVRAGKHVFME
                     KPVATDAPGIRRVLAAAEEAKAKKLNVVVGLQRRYDVQYRDWVERLHAGMIGDIILSR
                     VYWDSAGVWVHERATLEQEAGRKLTEMEYQMRNWYYFNWLCGDHICEQHIHNLDVGNW
                     VKQGYPVRAQGKGGRQVRTGIDHGQIYDHFVIEYEYADGSRMISSCRHQEGCWSAVTE
                     GFHGTNGTAPAPGRILSASGHTLFQHRGKDDPNPYQVEHDELFDAIVNGEYRFSDAEN
                     GAYATMTAIMGRMAAYSGQMVEWEQALASEVDIMPARYAWDADPPVMPDENGRYPIPM
                     PGQSQVI"
     gene            complement(1773..>2047)
                     /locus_tag="AC16YU_11075"
     CDS             complement(1773..>2047)
                     /locus_tag="AC16YU_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR6065812.1"
                     /translation="PEDGAASPWVVPTRLHPRTVRGGAYDDDPEAVRCASRLESNLNW
                     KRRDPQIPKSFWWNTDSPFVGFRLVRPAVPPPPEEIEFFWYQVLGG"
BASE COUNT          292 a          725 c          702 g          328 t
ORIGIN      
        1 ggtcggcgga tacctcgacg ggcacccccg gctcgtaggt ccgggcgatg cgggacacct
       61 cgccttcggg gtcgtagtcg ccgagcgcgg cctccatctc ccgaatcctg gcgaacgccg
      121 catcggcggc ctccgccccg gtccccgaat ccggcgcgca ggccacgatc cggaacagcg
      181 tgcccatcat cggccgctcg acgtccacgc ggacctggcc gctcgccgca ggggcggcgg
      241 ctgcggccag gaaacaggcc aggagcagtc gggcacgcgg gcgcgccgcg cggaagccct
      301 cagatgacct gggactgccc gggcatcgga atcgggtagc gtccgttctc atcgggcatg
      361 accgggggat cggcgtccca ggcgtaccgg gccggcatga tgtcgacctc ggaggcgagg
      421 gcctgctccc actcgaccat ctgcccggaa tacgccgcca tccggcccat gatcgccgtc
      481 atggtcgcgt acgcaccgtt ttccgcatcg gagaaccggt actcgccgtt gacgatggcg
      541 tcgaacagct cgtcgtgctc cacctggtag ggattcgggt cgtccttgcc ccggtgctgg
      601 aacagcgtgt gcccgctcgc cgacaggatg cggcccggcg ccggggccgt gccgttcgtt
      661 ccgtggaaac cctcggtgac ggcgctccag cagccttcct gatggcggca gctgctgatc
      721 atccggctcc cgtccgcgta ctcgtactcg atgacgaagt ggtcgtagat ctggccgtgg
      781 tcgatgccgg tgcgcacctg gcggccgccc ttcccctgcg cccgcaccgg gtagccctgc
      841 ttgacccagt tgcccacgtc caggttgtgg atgtgctgct cgcagatgtg gtcgccgcac
      901 agccagttga agtagtacca gttgcgcatc tggtactcca tttcggtcag cttgcggccg
      961 gcctcctgct cgagcgtggc ccgttcgtgc acccagacgc cggccgagtc ccagtacacg
     1021 cgggacagga tgatgtcgcc gatcatcccg gcgtgcagcc gctcgaccca gtcgcggtac
     1081 tgcacgtcgt agcggcgctg aaggcccacg acgacgttca gcttcttcgc cttcgcctcc
     1141 tcggcggcgg cgagcacgcg gcggatcccc ggggcgtccg tcgccaccgg cttctccatg
     1201 aacacgtgct tgcccgcgcg cacggcggcc tcgaaatgga gcggccggaa gccggggggc
     1261 gtggtcagga tgacgacgtc agccagcgga atcaccttct cgtacgcgtc gaagcccgtg
     1321 aaccggcgct ccggcgggac gtccacccgg tcccggatcg acgccgtatc gaacccggtg
     1381 tagtcggtgg cggcggggtc ggtgaggttg cggtggcact cttcgatccg gtcggcgaag
     1441 gcgtcggcca tcgccacgag gcgaacggac ggatgcgcgg tcagggcctg cgcggcggcc
     1501 ccggttcccc ggccgccgca ccctacgagt ccgacgcgga tcacctcgtc cgacgcgaaa
     1561 aaggcgctcg ccttgagcgg attcctgagg gccgcggcgc tgccgacgac ggcggccgtg
     1621 ctgcggacga aatcgcgtcg ggacatgccc gaccccggct tcctggtgtc catgacttcc
     1681 tgcgggattg cggcgtggag atgctccttc cggacgttag ccaccggagg tttcggccgc
     1741 aaatgctttt ccgctgaaga cccggccgcg actcacccgc cgagcacctg gtaccagaag
     1801 aactcgatct cctcgggggg cggcggcacc gcggggcgga ccaggcggaa tccgacgaac
     1861 ggcgagtcgg tgttccacca gaagctcttc ggaatctgcg gatcgcggcg cttccagttc
     1921 aggttggact cgaggcgcga ggcgcaccgg accgcctccg ggtcgtcgtc gtacgctccg
     1981 ccccgaaccg tccgtggatg cagccgcgtc ggaaccaccc acggcgacgc ggcgccgtct
     2041 tcgggca
//
LOCUS       DBNHQZ010000407         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1203708, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000407 DBNHQZ010000000
VERSION     DBNHQZ010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1203708"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1151)
                     /locus_tag="AC16YU_11080"
     CDS             complement(48..1151)
                     /locus_tag="AC16YU_11080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6065813.1"
                     /translation="MSRRKLYAGLVLAGAALAGGVWWLADPVPEAGIDRARRAITDAR
                     RERAEEFAPVEFAEATAAWDRVLSLVRTESETIAPLRSFDSVRAQVDVAVESARRARA
                     RAARVADSLETRLSRLGREADGQLARLERAADHYPISYVDRDALSQAREYRSHREGAA
                     RNGRLILAVQLAEKQAATVRRIQAGFEERLRPVVSQVPEWRASVRRLKAAAGRDPVVV
                     VDKAARTLYVHRAGRTDSFRVEFGRNWLGPKKMEGDDATPEGEYRVVRRLGRGETRYH
                     RALLINYPNESDRVRFERARTAGTIPRGARIGGSIELHGSGGRQVDWTDGCVALTNRD
                     MERVFAMVPVGSRVLIAGSLTTDLPYVHDTPDD"
     gene            complement(1226..1834)
                     /locus_tag="AC16YU_11085"
     CDS             complement(1226..1834)
                     /locus_tag="AC16YU_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HIR6065814.1"
                     /translation="MKHRIPAVMLLLVSAVLFAACAKAPEQALTAARDALTAARDAEA
                     DQYVPELYVAAADSFAAAEAEIETQNARNAFSRNYDRAEALLAFVTENAQVAQSRVAD
                     QKEMVRMENEALFATVDSTLARVADLMTQAPTGKDGTVALASIREDATLAGSSLAEAR
                     AAQEAGDYIRANAIAEAALEKGQALAAELVRAIEASGRRPRS"
     gene            complement(2103..>2901)
                     /locus_tag="AC16YU_11090"
     CDS             complement(2103..>2901)
                     /locus_tag="AC16YU_11090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6065815.1"
                     /translation="SFDGRRIQGWIVRPPDFDPGRRYPLILEIHGGPFAAYGAHFAAE
                     MQLYAAAGYVVLYTNPRGSTSYGEEFGNLIHHAYPGNDYDDLMSGVDAVVRLGYVDPE
                     QLFVTGGSGGGVLSAWIVGRTDRFRAAVVQKPVINWFSFVLYSDSPAFFHRYWFPGPP
                     WDHVEHYMARSPISLAGNVTTPTMLITGEQDHRTPMAESEQYYAALKIRGVEAALVRV
                     PDASHDIAAHPSNLVAKAAHVLAWFDRYRSPETDGVPRRTQGQPRGW"
BASE COUNT          438 a          976 c         1044 g          443 t
ORIGIN      
        1 ccaggcttcc cggcattacg gacctgcggg ccggggaacg gccgggatca gtcgtcgggg
       61 gtgtcgtgga catagggaag gtcggtagta agggatccgg cgatgagcac gcggctgccg
      121 acgggcacca tcgcgaagac gcgttccatg tcgcggttcg tcagggcgac gcagccgtcg
      181 gtccagtcga cctggcggcc tccgctccca tgcagctcga tggaaccgcc gatacgggcc
      241 ccccggggga tcgtcccggc ggtccgggcc cgctcgaatc ggacacggtc gctctcgttg
      301 ggatagttga tcaaaagcgc acggtgatac ctggtttcgc cgcgccccag gcggcggacc
      361 acgcggtact cgccttcggg tgtcgcgtcg tctccttcca tcttcttggg gccgagccag
      421 ttgcggccga actccacccg gaaggaatcc gtgcgccccg cacggtggac gtacagggtg
      481 cgggccgcct tgtcgaccac gacaaccgga tcgcgtccgg ctgccgcctt cagccggcgg
      541 accgacgccc gccattcggg cacctggctc acgacgggac gaaggcgctc ctcgaagcct
      601 gcctggatgc gtctcactgt ggcggcctgc ttttccgcga gctgcacggc caggatcagc
      661 ctcccgttgc gtgcggcgcc ttcgcggtgg gatcggtact cgcgggcctg ggagagggcg
      721 tcccgatcca cgtacgaaat gggataatgg tcggcagcac gctccaggcg cgcgagttgc
      781 ccgtcggctt cccgtccgag ccgggaaagc cgtgtctcca gggagtccgc cacccgggca
      841 gcgcgggcgc gggcgcggcg cgccgactcc acggcgacat ccacctgggc ccgcacgctg
      901 tcgaaggaac ggaggggcgc gatcgtctcg ctttcggtgc ggacgagcga gagcacccgg
      961 tcccaggcgg ccgtggcctc ggcgaactcc acgggcgcga actcctcggc gcgctcccgg
     1021 cgggcgtcgg tgatggccct gcgggcgcga tcgatgcccg cttccggcac cgggtcggcc
     1081 agccaccata cgccgccggc cagggccgcg cccgcaagta ccagtccggc gtacagcttt
     1141 cgtcggctca aggatgcggc cccggaaaat gggcaatgcc ccggccgccg ggcggtcggg
     1201 gcatcgccac gccatgaaac caggatcagc tgcgcggacg gcgtccggac gcctcgatcg
     1261 cacgcacgag ctcggccgcg agggcctgcc ccttctcgag ggctgcctcg gcgatcgcgt
     1321 tggcgcggat gtagtcgccg gcttcctgtg cggcgcgggc ctccgcgagg gaggatccgg
     1381 ccagcgtcgc gtcctcgcgg atggaggcga gcgcgacggt gccgtccttg ccggtcgggg
     1441 cctgcgtcat gaggtcggcc acgcgggcca gcgtcgaatc gaccgtggcg aagagcgcct
     1501 cgttctccat gcggaccatc tccttctggt cggccacgcg gctctgcgcg acctgggcgt
     1561 tctcggtcac gaaggccagg agagcctcgg cccggtcgta gttccgggag aaggcgttac
     1621 gcgcattctg ggtttcgatc tctgcttcgg ctgcggcgaa cgagtcggcg gcggccacgt
     1681 agagctcggg gacgtactgg tcggcctcgg catcccgggc ggccgtgagg gcgtcgcggg
     1741 cggcggtgag ggcctgctcc ggtgccttcg cgcacgccgc gaagagcacg gccgacacga
     1801 ggaggagcat gacagcggga attcggtgtt tcatcgacgg atcaggtatg gttctctcgg
     1861 gggagagcgg tttttgatcg ccgggcggga aaacggttcc ggccggaaca ggccagggcg
     1921 ggtatcgcaa cccccggctg cgaccctctc ggggtcgtag aagaattgaa cgcccacgga
     1981 tcggggggtt tcataccgtc gattagagat ggattaaagc cattcgtccg ccgatcgggc
     2041 ggcggtaccg taccgggagc gaccaatcgg gcggtagcac cgacgccgga acgcccccgg
     2101 gatcaccatc cgcgcggctg cccctgcgtc cgccgcggca cgccgtcggt ctccggtgac
     2161 cggtaccggt cgaaccaggc gagcacgtgg gcggccttgg ccacgagatt cgacggatgg
     2221 gcggcgatgt cgtgcgacgc gtccggcacc ctcaccaggg ccgcctctac cccccggatc
     2281 ttcagcgcgg cgtagtactg ctccgactcg gccatggggg tgcgatggtc ctgctcaccg
     2341 gttatgagca tggtcggcgt cgtgacgttt cccgcgagcg agatcggcga gcgcgccatg
     2401 tagtgctcga catggtccca cggcgggccg gggaaccagt agcgatggaa aaaagccggg
     2461 ctgtcggagt acaggacaaa gctgaaccag ttgatgaccg gcttctgcac gaccgccgcc
     2521 cggaaccggt ccgtccggcc cacgatccac gcggacagga cgccgccgcc cgagccgccg
     2581 gtcacgaaga gctgctccgg atccacgtaa ccgagacgga ccaccgcgtc cacgcccgac
     2641 atcaggtcgt cgtagtcgtt gccgggatag gcgtgatgga tcaggttgcc gaattcctct
     2701 ccgtagctcg tggaaccccg cgggttcgtg tagagcacga cgtaccccgc ggccgcgtag
     2761 agctgcattt cggccgcgaa atgcgcgccg taggccgcga acgggccgcc gtggatctcg
     2821 aggatcaggg ggtagcgacg cccggggtcg aaatccgggg gacgcacgat ccagccctgg
     2881 atccgccggc cgtcgaacga c
//
LOCUS       DBNHQZ010000408        10247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1071997, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000408 DBNHQZ010000000
VERSION     DBNHQZ010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1071997"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..896)
                     /locus_tag="AC16YU_11095"
     CDS             complement(183..896)
                     /locus_tag="AC16YU_11095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6065816.1"
                     /translation="MGHPEHVVLVTGASRGIGAATARAFAGLPRTALALVSRDRSALE
                     ASAVDCRAAGAAADVFVCDLTDESEVHRMALAVTERFGLPTVVVNNAGLFAPGGVADT
                     DPAAFREQVDANLFTAFVVTHSLLPAMLERGSGHFLFMGSVASIRGYARGAAYCAAKH
                     GLLGLARALREETRDRGLRVTTLLAGATWTDSWAGADLPEERFMPAEDIAAAARDAVL
                     MSDRTVVEEILIRPQLGDV"
     gene            complement(899..1504)
                     /gene="folE"
                     /locus_tag="AC16YU_11100"
     CDS             complement(899..1504)
                     /gene="folE"
                     /locus_tag="AC16YU_11100"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR6065817.1"
                     /translation="MEAEANLYEILHRYDPEATTAIAGHVHGILRRIGEDPDREGLVK
                     TPERVAKAWQFLTHGYGQDPKAILESALFAEEYSEMILVRDMEVYSVCEHHMIPFFGK
                     AHVAYIPNGHIVGLSKIPRVVDVFARRLQVQERLTIQIRDAIEEVLKPVGVAVVIEAT
                     HLCMVMRGVQKQHSIATTSAMSGEFLNSTDTRAEFMRLISR"
     gene            complement(1529..1942)
                     /locus_tag="AC16YU_11105"
     CDS             complement(1529..1942)
                     /locus_tag="AC16YU_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HIR6065818.1"
                     /translation="MPIVYATRRVHFNAAHRMHNPEKSDEWNRTTFGECNHPNWHGHN
                     YVLDVTVAGEPDPETGYLIDLGELKRIVQERVLRHVDHRNLNLDVEFLRGILPSTENF
                     AVAIWRRLEDALPAGRLHSVRLYESERNVVEYRGE"
     gene            2114..2458
                     /locus_tag="AC16YU_11110"
     CDS             2114..2458
                     /locus_tag="AC16YU_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6065819.1"
                     /translation="MAEKTLFQKIADREIPADFVYEDDEIVAFRDIDPKAPVHVLVVP
                     RKPIPTLDDLEPADAALVGRMFLVAKQVAAAEGLRGGWRAVFNCGREAGQAVFHIHLH
                     VLGGRKMSWPPG"
     gene            2469..4490
                     /locus_tag="AC16YU_11115"
     CDS             2469..4490
                     /locus_tag="AC16YU_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065820.1"
                     /translation="MPAVRPSFLAALVLIGIVAALPASARQDGRYEAGFERDVNRYRW
                     SGGLALAVPVAGWSLRLSEAFRSDAYLLFDDRLTFRDENAVAIDALRASSARVRPVVR
                     GRSNWYSLSRVLAQSLTGGILWEPARGVTLEPAAGVAMDRRPGVAQPGGVAPMRTDVG
                     PAVALRLDVPRRTVGGYTVRAEGGAGWQRIDPRRAGGLRLRGDAGRTFERTTVHAEVL
                     FASARRDAYQAASFLNRDEALSRAAESIESTRSDTATATLRVDAPVSEGLTLGSRVIL
                     SANNRAIRTHSAPLDALFFDTDFRRQSAEAELSARYARRGVELRASVEGGAETERRTL
                     TNEDDLPGPQAAQKRDLLRQADYDRGFVMLRLDVRVPLGRRLIFQADGSANGIRHDTP
                     IINADDRDEAFYSGRVGLLFRADRTLEADLQVFGSWYHTVYLKAARSAENNVQRSLRL
                     RPAVRWRPSRATRLELSSEVRATYTVDDFVLPGRQPTDQAAREMRYDLTVEQDLGSGL
                     RIVADGRYSDLRLGRFLDQVFAEIPYDTLRTYGGWLRLRTGGRVTGEIGLRFFMRTDY
                     DRSATVRYVRLGEDGTVLVEPDGRPQTTTISRPGRRSILQFGPTCAVVFPLRGGAFLR
                     FDGWMTVQRVTARLYGDLPEESADIIRRAASRGTRTLIPNLSVSMQWTF"
     gene            4504..5685
                     /gene="coaE"
                     /locus_tag="AC16YU_11120"
     CDS             4504..5685
                     /gene="coaE"
                     /locus_tag="AC16YU_11120"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6065821.1"
                     /translation="MKTLGVTGGIGSGKTTVCRLLEERGAEVFYADEVGKRILQEDPE
                     ARRELVEAFGERAYAADGRLDRAYVASRVFGDAGEVDRINAIVHPRVHAAFDLARRDA
                     AARGVRVLVKEAALLLDSGGKGGLDAILVVTAPEADRVRRVAGRDGSDPADVRLRMAH
                     QRSDASFREAADFVIENDGSLQELEARVDAVWRELVSRPRWPEVPPDLPRRGNAFTRW
                     FGRAGLRLLRFRVVADLPNLKQFVAIGAPHTTNWDFVLAMLMLMALGLRIHWIGKHTI
                     FRWPFGGLMRALGGIPVDRSEPGDIIDQVVARFRAEERFAVGLSPEGTRRRAERWKSG
                     FYRIAVGAGVPIVLGVIDFRRRELRLDSVFHPTGDFEADLPLIRRVYRAEQAKYPELF
                     A"
     gene            complement(5758..8871)
                     /locus_tag="AC16YU_11125"
     CDS             complement(5758..8871)
                     /locus_tag="AC16YU_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6065822.1"
                     /translation="MIGQTVAHYRIDSRLGSGGMGVVYRAFDLQLKRPVALKFLPPHL
                     SADEDAKQRFMQEAQAASALNQANICIVHHIGETDDGQLYIVMAFYEGQTLKYRLRDG
                     ALPEPLALDLARQMAAGLARAHEAGIVHRDIKPANIMVTDRDEVRILDFGVAKLESSA
                     DFTKAGTTVGTAAYMSPEQTRGGRVGPAADVWSLGVVLYEMLSGKRPFDAGYDQALAY
                     AITNEEPAPLAGVSPETAALVRRMLAKDPAGRPADGSAALAALGGRADPAGGSAPSPS
                     PARDRAAAPSLARTVGLFAAGSIATLAIVYAAMIRFGLPDWVLTLGIVLLLAGLPVLV
                     FATRAESRRAGLDSGERRSLSGLPSWITVRRAWIGGGLAMAGLVVVSAGWMGLRALGI
                     GPAGTLLTRGVLTESDRVIVAEFEDRTDDGTLGRTIAQALRIDLAQSPVVSLMDDAAV
                     RAALERMQRDPSTRIDLAVAREVAQREGAKAVIAGEVAPLGSSYVISLRLLDAAGGQE
                     LAPLRETAESDATIVQAVDRLSRALRERIGESLRTIRAGAPLEQVTTTSLEALRLYTA
                     GVNAFDRRDFEEAIENLEEAIAIDSTFGMAYRKLAVIYWNEGRNREQQFRLSTRTYEL
                     RTRLPERERFMAEAWYHVAVGRDQEAALRVYRGMLERFPNDQTALNNAAVAALALKRP
                     DEAARYLRQGIRTAADPISNLYQNLVTALTDAGRIDEAEAALDSLSAVFPDRVLVGNI
                     ASMVRTSRTGDYRAEADSIDALIAIETDADVLRSLSSVRVRFAQYLGRLAEAERLARQ
                     VEEMRPSDAPDALVLERELDALIARLAATGDRDAAAARLEALLARYPLAGLAPLDRPY
                     SMLAYVNAQLGRTAETRRLLDEQARLWPGGWVRWDFVRYLALGLAALADEDPAAAVER
                     IREGLPYGPGFGAAACWRCGAYELGLALRAAGDAEGAARVLNDALGRPAAIETTNDLG
                     TITLAWRLLGDVSETLGRTDDAIRAYERFLELWADADPELQPQVADVRGRVDRLLAAR
                     SRESDGS"
     gene            complement(8906..9829)
                     /locus_tag="AC16YU_11130"
     CDS             complement(8906..9829)
                     /locus_tag="AC16YU_11130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6065823.1"
                     /translation="MPQWVRIALTVGLLALVAANVDAYAMAAALRDASWLPVAVALAL
                     VPFNWALAGVSWLLIGRRIDPGLRYGEAMSVVLAGHAVAIWTPARVAEFAGRAYLHGR
                     GKATAWMAGTGLETFLRYAPPMLIGGIALGFVDAQPAFPWQVARLVGAAGLVLIVGVA
                     MFPERLRRFLSRRRERPALAFLSTTSRGDILAVLGLNAARLLTGSVQMTLFAVAFRAG
                     ASPMVLFAACASALSVKTAIPPVTVGDLGIREAASVVLLGAVGVSAPVALNAALFVYL
                     ANVIVGAVVGALVWATRGNRLDGRPPVEKAS"
     gene            complement(9870..10181)
                     /gene="yajC"
                     /locus_tag="AC16YU_11135"
     CDS             complement(9870..10181)
                     /gene="yajC"
                     /locus_tag="AC16YU_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6065824.1"
                     /translation="MSTLTFFLLGAPAGEQANPLAMFLPLVLIFLVFYFFIIRPQKKK
                     EDNRKKMIEAVRKGDKVVTIGGIHGTVMQVDETSVLLQADANTKLRVEKNALSNVSGK
                     D"
BASE COUNT         1410 a         3644 c         3742 g         1451 t
ORIGIN      
        1 gggcgtggtt catggcgccc gggagcctgg gcgaggtctt cgcatcggag ccggagaccc
       61 tgtggcggcg cgtgctcgtc cgcctgggcg gccggttcgc gctcctggtc aattatccgg
      121 acgatccgcg gctcaactga ggcccgccgc gccgcccggg tggacaggtc ccgggcgtcg
      181 gttcacacgt cgccgagctg cggccggatc aggatctcct cgaccaccgt ccggtcggac
      241 atgagcacgg cgtcccgggc ggccgccgcg atgtcttcgg ccggcatgaa gcgctcctcg
      301 ggcaggtccg cgccggccca gctgtccgtc caggtggcgc ccgcgaggag cgtcgtgacc
      361 cggagcccgc ggtcgcgggt ctcttcccgc aacgccctcg cgagacccag gagcccgtgc
      421 ttggccgcgc agtaggcggc gccgcgggca tatccccgga tggacgccac cgagcccatg
      481 aacaggaagt gtccggaccc cctttcgagc atcgcgggca gcagcgagtg cgtgacgacg
      541 aaggccgtga acaggttcgc gtccacctgc tcgcggaagg ccgccgggtc ggtgtccgcc
      601 acgccgcccg gtgcgaagag gccggcgttg ttcacgacga cggtcggcag gccgaaccgc
      661 tccgtgacgg cgagagccat ccggtgcacc tcgctttcgt cggtcaggtc gcagacgaag
      721 acgtccgccg cggcgcctgc ggcccggcag tccacggcgg aggcctcgag cgcggagcgg
      781 tcgcgggaca cgagcgccag ggccgtccgc ggcaggccgg cgaacgcccg ggcggtggcc
      841 gcgccgatgc cccggctcgc gcccgtcacg agaacgacgt gttccggatg gcccatggtc
      901 atcggctgat cagtcgcatg aactcggcac gcgtatcggt ggaattgagg aactcgccgc
      961 tcatcgcgct ggtcgtcgcg atcgagtgct gcttctgcac gccgcgcatc accatgcaca
     1021 ggtgggtcgc ctcgatcacg accgccacgc cgacgggctt gagcacctcc tcgatggcgt
     1081 cgcggatctg gatcgtgagc cgctcctgca cctgcagccg ccgggcgaac acgtccacga
     1141 cgcgcgggat cttgcttagc cccacgatgt ggccgttcgg gatgtaggcc acgtgcgcct
     1201 tcccgaagaa cgggatcatg tggtgctcgc acacgctgta gacctccatg tcccgcacca
     1261 ggatcatctc ggagtattcc tcggcgaaga gggcgctctc caggatggcc ttcggatcct
     1321 gcccgtatcc gtgggtcagg aactgccagg ccttggcgac gcgctctggc gtcttgacca
     1381 ggccctcgcg gtcggggtcc tctccgatgc ggcggaggat gccgtgcacg tgacccgcga
     1441 tggcggtggt ggcctcgggg tcgtaccggt gcaggatttc gtacagattg gcttcggctt
     1501 ccacgaaaag gctcggatgg tttacggact actcgccgcg gtactcgacc acgttccgct
     1561 cggactcgta caggcggacc gaatgcagcc ggccggccgg cagggcgtcc tcgagacggc
     1621 gccagatggc gaccgcgaag ttctcggtgg acggcaggat gccccggagg aattcgacgt
     1681 cgaggttcag gttgcgatgg tccacgtgcc ggaggacgcg ctcctggacg atccgcttca
     1741 gctcgcccag gtcgatcagg tagccggtct ccggatcggg ctcgccggcc accgtcacgt
     1801 cgagcacgta gttgtgcccg tgccagttcg ggtggttgca ctcgccgaac gtggtccgat
     1861 tccattcgtc cgatttctcc ggattgtgca tccggtgcgc ggcgttgaaa tgtacccggc
     1921 gggtcgcgta gacgatcggc atggagggcg tcgttcgttg tcgtaacaaa cgtctaccgt
     1981 ccggccttgt tgcatccgcg gcccgctgcg taccgcccgc cgcggcccgc cgcgccgcgc
     2041 gggatgcgcc gcgcggcccc tccggcggcg gccgcgctcg cctatattcc gggcgtgcag
     2101 cccacgcgat gccatggccg aaaagacgct cttccagaag atcgccgacc gggagatccc
     2161 ggccgacttc gtgtacgagg acgacgagat cgtggccttc cgggacatcg atccgaaggc
     2221 gcccgtccac gtgctcgtgg ttccgcgcaa gccgatcccg acgctcgacg acctggagcc
     2281 cgccgacgcc gcgctcgtcg ggcggatgtt cctcgtggcg aaacaggtcg cggccgcaga
     2341 gggcctccgc gggggatggc gcgcggtgtt caactgcggg agggaggccg gacaggccgt
     2401 cttccacatc cacctgcacg tgctgggcgg ccggaagatg agctggcccc ccggctgatc
     2461 ccgccgccgt gcccgccgtc cgcccgtctt tcctggcggc cctggtcctg atcgggatcg
     2521 tcgccgcgct gcccgcctcc gcccggcagg acggccgcta cgaggccggc ttcgagcgcg
     2581 acgtgaaccg ctaccggtgg tccggcggac tggccctggc ggtgcccgtc gccgggtgga
     2641 gcctgcgcct gtcggaagcc ttccgctcgg acgcctacct gctgttcgac gaccggctca
     2701 cgttccggga cgagaacgcc gtggccatcg acgccctgag ggcctcgtcg gcgcgcgtgc
     2761 gcccggtcgt gcgcggccgg tccaactggt acagcctgag ccgcgtcctc gcgcagtcgc
     2821 tgaccggcgg catcctgtgg gagccggcgc ggggcgtgac cctggaaccc gcggccggcg
     2881 tggcgatgga ccggcggccc ggggtggcac agccgggcgg cgtggcgccc atgcggaccg
     2941 acgtgggtcc cgcggtggcg ctccgcctgg acgtgccccg gcggaccgtg ggcgggtaca
     3001 cggtgcgggc ggagggcgga gccggttggc agcgcatcga tccgcgccgg gcgggcgggc
     3061 ttcggcttcg cggcgacgcc gggcggacgt tcgagcggac cacggtgcac gcggaggtgc
     3121 tcttcgcctc ggcgcggcgg gacgcctacc aggcggcctc cttcctgaac cgcgacgagg
     3181 ccctctctcg cgccgcggag tcgatcgagt cgacccgcag cgacaccgcc acggcgacgc
     3241 tccgcgtgga cgcccccgtc tcggagggcc tcacgctcgg ctcgcgggtg atcctgtcgg
     3301 cgaacaaccg ggccatccgg acgcacagcg cgccgctcga cgcgctcttc ttcgacacgg
     3361 acttccggcg gcagtcggcg gaggccgagc tgagcgcccg gtacgcgagg cggggcgtgg
     3421 agctccgggc atccgtcgag ggcggcgccg agaccgagcg gcgcacgctc acgaacgagg
     3481 acgacctgcc cggcccgcag gccgcccaga agcgggacct cctgcggcag gccgactacg
     3541 accgcggttt cgtcatgctg cgcctggacg tgcgcgtccc cctcggccgg cggctcatct
     3601 tccaggcgga cggctcggcg aacggcatcc ggcacgacac gccgatcatc aacgcggacg
     3661 accgggacga ggccttctac agcggccgcg tggggctgct gttccgggcg gaccgcaccc
     3721 tcgaggcgga cctccaggtg ttcggctcct ggtaccacac ggtgtacctg aaggcggccc
     3781 ggtccgccga gaacaacgtg cagcgcagcc ttcgcctgcg cccggccgtc cgctggcggc
     3841 cgtcgcgggc gacgcgcttg gagctgtcgt ccgaggtccg ggccacgtac acggtcgacg
     3901 acttcgtgct gcccggccgg cagccgaccg accaggcggc ccgcgagatg cggtacgacc
     3961 tgacggtcga gcaggacctc ggctcggggc tccgcatcgt ggccgacgga cggtactcgg
     4021 acctgcggct ggggcgcttc ctggaccagg tcttcgccga gatcccctac gacacgctgc
     4081 gcacgtacgg gggctggctg cggctccgga ccggcgggcg cgtgacgggc gagatcggcc
     4141 tgcgcttctt catgcggacc gactacgacc gctcggcgac cgtccgctac gtgcgtctgg
     4201 gcgaggacgg caccgtcctg gtcgagcccg acggccgccc gcagacgacc acgatcagcc
     4261 gccccggacg gcgcagcatc ctgcagttcg ggccgacctg cgccgtggtg tttccgctgc
     4321 ggggcggcgc cttcctgcgc ttcgacggct ggatgaccgt gcagcgcgtg acggccaggc
     4381 tctacggcga cctgcccgaa gagtcggccg acatcatccg tcgcgccgcg tcgcggggca
     4441 cgcgtacatt gattccgaat ctttccgtct ccatgcaatg gaccttctga ggccggcgat
     4501 cccatgaaga cgctcggcgt taccggcggg atcgggagcg ggaagacgac cgtgtgccgc
     4561 ctgctcgagg agcggggggc cgaggtcttc tacgcggacg aggtcggcaa gcgcatcctg
     4621 caggaggatc cggaggcgcg gcgcgagctc gtcgaggcct tcggcgagcg ggcgtacgcc
     4681 gccgacggcc ggctggatcg tgcgtacgtg gcgtcccgcg tgttcggcga cgccggggag
     4741 gtcgacagga tcaacgcgat cgtgcatccg agggtgcacg cggccttcga cctcgcgcgg
     4801 cgcgacgccg cggcccgcgg cgtgcgggtg ctcgtgaagg aggccgcgct gctcctggac
     4861 tcgggcggga agggcggact ggacgcgatc ctcgtggtca ccgctccgga ggccgaccgc
     4921 gtgcgccgcg tcgcggggcg cgacggctcc gacccggccg acgtccggct ccggatggcg
     4981 caccagcgat cggacgcgtc gttccgcgag gcggcggact tcgtcatcga gaacgacggc
     5041 tcgctgcagg agctcgaggc gcgggtagac gccgtatggc gggagctcgt gtcccgtccc
     5101 cgctggcccg aggtgccgcc ggacctgccg aggcgcggca acgccttcac ccggtggttc
     5161 gggcgcgccg ggctgcgcct tctccgcttc cgcgtggtcg cggacctccc gaacctgaag
     5221 cagttcgtgg cgatcggcgc gccgcacacc acgaactggg acttcgtgct cgccatgctg
     5281 atgctcatgg cgctcggcct caggatccac tggatcggga agcacacgat cttccggtgg
     5341 ccgttcggcg gcctgatgcg ggcgctcgga gggatcccgg tggaccgctc cgagccgggc
     5401 gacatcatcg accaggtcgt ggcgcggttc cgcgcggagg aacgcttcgc ggtaggcctc
     5461 tcgcccgagg gcacccgccg ccgcgccgag cggtggaagt cgggcttcta caggattgcc
     5521 gtcggggccg gcgtccccat cgtcctcggg gtgatcgact tccgccggag ggagctcagg
     5581 ctggactcgg tgttccaccc gacgggcgac ttcgaggccg acctcccgct gatccggcgg
     5641 gtgtaccgtg cggagcaggc gaagtacccg gagctcttcg cttagggagg tggcggggcg
     5701 gtgcgccccg atcgtcggcc cgccggcgtt cccgccacgc ggtccggttg ccccgcccta
     5761 cgacccgtcg gactcgcgcg agcgcgcggc gaggagccgg tccacccgcc cgcgcacatc
     5821 ggccacctgc ggctgcagct cggggtccgc gtccgcccag agctccagga agcgttcgta
     5881 ggcccggatc gcatcgtcgg tgcggccgag cgtctcgctc acgtcgccga ggaggcgcca
     5941 ggccagggtg atggtcccga gatcgttcgt ggtctcgatc gcggcgggcc ggccgagtgc
     6001 gtcgttcagc acccgggccg ccccctcggc gtcgcccgcc gccctgagcg cgaggcccag
     6061 ctcgtatgcg ccgcagcgcc agcacgcggc cgcgccgaac cccggaccgt acggcaggcc
     6121 ctcgcggatc cgctccaccg cggccgccgg gtcctcgtcc gcgagcgcgg cgaggccgag
     6181 cgccaggtac cgcacgaaat cccaccggac ccacccgccc ggccacaatc tggcctgctc
     6241 gtccaggagg cgacgcgtct cggccgtccg cccgagctgg gcattcacgt aggccagcat
     6301 cgagtagggc cggtccaggg gcgccaggcc cgcgaggggg taccgggcga gcagggcctc
     6361 gagccgcgcc gccgccgcgt cccggtcgcc ggtggcggcc aggcgggcga tgagggcatc
     6421 gagttcccgc tccaggacca gggcgtccgg ggcatccgac ggccgcatct cctccacctg
     6481 gcgggcgagg cgctcggcct cggcgagccg tcccaggtac tgggcgaagc gcacgcgcac
     6541 ggacgaaagc gaccgcagga cgtccgcgtc cgtttcgatg gcgatcaggg catcgatcga
     6601 atcggcctcc gcccggtagt cgccggtccg gctggtgcgc accatcgagg cgatgttgcc
     6661 cacgaggacc cgatccggga agaccgcgga caacgagtcc agggcggcct cggcctcgtc
     6721 gatgcgtccg gcgtcggtga gcgcggtgac gagattctgg tacaggttcg atatcgggtc
     6781 cgccgccgtg cggatgccct gcctcaggta gcgggcggcc tcgtccgggc gcttgagcgc
     6841 gagcgcggcc acggccgcgt tgttcagggc cgtctggtcg ttcgggaagc gttccagcat
     6901 gccgcgatac acccggagcg cggcctcctg gtcccgtccc acggcgacgt ggtaccaggc
     6961 ttcggccatg aaacgctccc gctccggaag gcgcgtgcgg agctcgtacg tgcgcgtcga
     7021 cagccggaac tgctgctccc ggttcctccc ctcgttccag tagatgacgg ccagcttccg
     7081 gtaggccatg ccgaaggtcg aatcgatcgc gatcgcctcc tccaggttct cgatcgcttc
     7141 ctcgaaatcc cgccggtcga aggcgttcac gccggccgtg tacaggcgga gcgcctcgag
     7201 ggacgtcgtc gtcacctgct ccagcggcgc gccggcccgg atggtgcgga gcgactcccc
     7261 gatccgctca cgcagcgcgc gagagagccg gtccacggcc tgcacgatgg tcgcgtcgct
     7321 ctcggcggtc tctcgcaggg gggcgagctc ctgcccgccg gccgcgtcca gcaggcgcag
     7381 gctgatgacg tagctggagc cgagcggcgc cacctcgccg gcgatgaccg ccttggcccc
     7441 ctcgcgctgc gccacctcgc gggccacggc caggtcgatc cgggtcgagg ggtcgcgctg
     7501 catgcgttcg agcgcggccc ggacggcggc gtcgtccatg agcgatacga cgggcgactg
     7561 ggccaggtcg atccgcaggg cctgcgcgat ggtgcggccg agcgtgccgt cgtcggtccg
     7621 gtcctcgaat tcggcgacga tgacgcggtc cgactccgtg agcacgccgc gggtgagcag
     7681 ggtgcccgcg ggcccgatgc cgagcgcccg cagccccatc caccccgccg agacgaccac
     7741 gagaccggcc atggccaggc cacctccgat ccacgcccgc cgcaccgtga tccacgacgg
     7801 aagcccggag agcgagcgcc gctcgcccga gtccaggccg gcgcgccggg actcggcgcg
     7861 ggtcgcgaag acgaggaccg ggaggccggc aaggagcagg acgataccga gcgtcagcac
     7921 ccagtccggc aggccgaacc ggatcatggc cgcgtagacg atcgcgaggg tcgcgatcga
     7981 gccggccgcg aagaggccga ccgtgcgcgc gagcgagggc gcagccgcgc ggtcccgcgc
     8041 cggcgacggc gacggggcgc tgccgcccgc cgggtcggcg cgtcctccga gggcggccag
     8101 cgcggccgag ccgtcggcgg gccggccggc cgggtccttg gcgagcatgc gccggacgag
     8161 cgcggccgtc tccggcgaga cgccggcgag cggggcgggc tcctcgttcg tgatggcgta
     8221 ggccagggcc tggtcgtacc cggcgtcgaa gggacgcttg cccgacagca tctcgtacag
     8281 gaccacgccc agcgaccaga cgtccgcggc gggtccgacc cggcctccgc gcgtctgctc
     8341 agggctcatg taggccgcgg tcccgacggt cgtccccgcc ttggtgaagt cggccgacga
     8401 ctcgagcttg gccacgccga agtccaggat ccggacctcg tcgcggtccg tgaccatgat
     8461 gttggccggc ttgatgtccc ggtggacgat gccggcctcg tgcgcgcggg cgagcccggc
     8521 ggccatctgc cgcgccaggt ccagggccag gggctccgga agcgcgccgt cgcgcagccg
     8581 gtacttgagc gtctgcccct cgtagaaggc catgacgatg tacagctgcc cgtcgtcggt
     8641 ctcgccgatg tggtggacga tgcagatgtt cgcctggttc agcgccgacg cggcctgcgc
     8701 ttcctgcatg aaccgctgct tcgcgtcctc gtcggccgac aggtgcggcg gcaggaattt
     8761 gagcgcgacc ggccgcttga gctgcaggtc gaaggcgcgg tacacgacgc ccatgccgcc
     8821 acttccgagg cgggaatcga tgcggtaatg ggcgacggtc tggccgatca tcggtctggg
     8881 ggtagcggcc tgtagatacg gaaattcacg atgccttctc aacaggcggt cgcccgtcga
     8941 gtcgattccc ccgggtcgcc cagacgagcg cccccaccac cgcgccgacg atcacgttgg
     9001 ccaggtacac gaagagcgcg gcgttcaggg cgacgggcgc cgacacgccc acggccccga
     9061 gcaggacgac ggacgcggcc tcgcggatgc ccaggtcccc gaccgtcacc ggcgggatcg
     9121 ccgtcttgac cgagagcgcg gacgcgcacg ccgcgaaaag caccatcggc gaggccccgg
     9181 cccggaaggc gaccgcgaag agcgtcatct ggacggatcc cgtgaggagc cgcgccgcgt
     9241 tcaggcccag gaccgcgagg atgtccccgc ggctcgtagt cgagagaaag gccagcgcgg
     9301 gccgctcgcg ccgccgtgac aggaagcgcc ggagccgctc ggggaacatc gcgacgccca
     9361 cgatcaggac gagtcccgcg gcgcccacca gccgcgccac ctgccagggg aaggcgggct
     9421 gggcgtccac gaagcccagc gcgatgccgc cgatcagcat cggcggcgcg tagcggagga
     9481 aggtctcgag gcccgtcccg gccatccagg ccgtcgcctt gccccgcccg tgcaggtacg
     9541 cgcgtcctgc gaactccgcc acgcgcgccg gcgtccagat ggccaccgcg tgccccgcca
     9601 ggaccacgct catggcttcg ccgtagcgca gcccgggatc gatgcggcgg ccgatcagga
     9661 gccaggagac gccggccagc gcccagttga atggaacgag cgcgagggcg accgcgaccg
     9721 gcaaccaact cgcgtcacgc agggcggccg ccatcgcgta cgcgtcgacg ttcgcggcga
     9781 ccagggccag cagcccgacc gtcagcgcga tccggaccca ctgggggatg cggcgcgacc
     9841 ggcgcgcggc cggggcgccg ggggcgggat cagtcctttc cggagacgtt ggagagggcg
     9901 ttcttctcga cgcgcagctt ggtgttcgcg tccgcctgta gcagcacgct ggtctcgtcc
     9961 acctgcatga cggtgccgtg gatgccgccg atggtgacca ccttgtctcc cttgcggacg
    10021 gcctcgatca tcttcttgcg gttgtcctcc ttcttcttct gcggccggat gatgaagaaa
    10081 tagaagacca ggaagatgag gacgaggggc aggaacatgg ccagcggatt ggcctgttcg
    10141 ccagccgggg caccaagaag gaagaaagtc agggtcgaca tgttcggatg gtatcgttga
    10201 gcggtggacc aatatacggg cttcgggggg cgcagacgag aaatcgt
//
LOCUS       DBNHQZ010000409        10158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_255664, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000409 DBNHQZ010000000
VERSION     DBNHQZ010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_255664"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..413)
                     /locus_tag="AC16YU_11140"
     tRNA            complement(341..413)
                     /locus_tag="AC16YU_11140"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(377..379),aa:Glu,seq:ctc)
     gene            complement(574..1644)
                     /locus_tag="AC16YU_11145"
     CDS             complement(574..1644)
                     /locus_tag="AC16YU_11145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6065825.1"
                     /translation="MRLLRLLPAIALVLALSSCDYTETERIVGGVNLDVLFATPSLSE
                     ISAIEAEWALRDVSARTVRIEHIKSVGTGDARFLVQVVSHVVEGTKHYGGIVTPDGAA
                     PGSLPVLVYLHGGDGGTSLDDEVLPAIETIGSAIGDMVMVVPSFRSETLRFDGRTFTS
                     EGPPSPWDRDVDDAMALLSAAFDVAPAADPERVAALGLSRGGGVALLMGARDERVDRV
                     VEFFGPTDFFGSYVQAIVEEALDGNPPDLPGVSYLDSEFIQPLADAMLSIGDVRPELV
                     RRSAVLFVDSMPPVQVHHGTADPVVDVSQAESLIRAMQRAGKPAAEFESYLYQGGGHN
                     ALTLDGSITRTAEFLAPLWVVP"
     gene            1866..3020
                     /locus_tag="AC16YU_11150"
     CDS             1866..3020
                     /locus_tag="AC16YU_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR6065826.1"
                     /translation="MADPIYLDYNATTPVDPRVLEHMLPYFTERFGNPSSKRHAYGWA
                     ADEAVERAREQVAGLLGAEPHEILFTSGATEAANLGIKGYAETMASRGRHVVTCATEH
                     KAVLEACRSLARRGFEVTVLPVDADGLLQPDVVEEALRDDTVLVAVMWANNETGVVHP
                     VARIAERVHARGIALFCDATQAVGKVPVDAGHVDLLACSSHKFYGPKGTGALFVARRE
                     RRLRVAPQIEGGGQESGLRGGTLNVPGIVGIGAAAEIAGEDMEADGSRLEALRDEIER
                     SVGRGFPDLRVNGRGAPRLPNTSSMTFHGLSAADLIAGTRDLALSTGSACSSGTGKPS
                     HVLQAMGLSDADGAATIRLSVGRFTTADEASAAAERLASVASSLLERTPA"
     gene            3094..3615
                     /locus_tag="AC16YU_11155"
     CDS             3094..3615
                     /locus_tag="AC16YU_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HIR6065827.1"
                     /translation="MEPIVNRVAQSGIEVLDLSMFEPDGDPVELDIAPFLVEGLILRE
                     KPFREAVAAFDFSPFAGRLVAVRCSADAIVPTWAWMLVATRLDGIATAVTVGSPADAM
                     REHFARAVAAFDWSRYEDRTVVVKGCGSGRVPEAAYVMATTALQRVARKVMFGEPCST
                     VPLWRRGSGGRGS"
     gene            3622..4761
                     /locus_tag="AC16YU_11160"
     CDS             3622..4761
                     /locus_tag="AC16YU_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR6065828.1"
                     /translation="MQPATVPAAEVSVPGGRLGQRLFALYRRHERIAPALFFFAGIGW
                     DAATVFRIDALIDNVLVALYLAALGVLVTASALDGAGQLRSRFLARWRPWFPAAMQFF
                     LGALFSVFVFFYSQSASLSQTSVYLGLLVALLVANEFLHRRMLDLHLGLGLYYLALAS
                     FLVYFVPVVTGRMGTAMFLLGTLAAAGAVLAIVSLLRRRGVFASARQAASVSALVVVL
                     FGFHQAAYHLNWIPPVPIALRDAGIYREVVREGDTYRMRFAPQPWWMFWRRDERHLDW
                     RPGEPVYCFTAVFAPTRLESRIVHVWEHLDEATGEWAESDRIGYDIVGGRDYGYRGYT
                     VKRTMRPGRWRVRVETSGGRLLSRIPFTVREAEPADTAWTWITAR"
     gene            complement(4889..5512)
                     /locus_tag="AC16YU_11165"
     CDS             complement(4889..5512)
                     /locus_tag="AC16YU_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6065829.1"
                     /translation="MKREERNGRRSFQPTSGIIVAAAVTVMLFGCDQQGSLGDEPELN
                     AQQTTFFAGKAAASALLSSADYIGVQGVTVEPLARGGFPDKIDGLFRVNLAGSPGTTV
                     ADIHDSKDVVVAKLTFAPHGFVGWHSHPGPAIVTISAGELTIVNESDCVPRLYAAGKA
                     FLDPGQGNVHVAVNDTEAEVVVYATFIDIPPGKGPTELLDTPGDCSL"
     gene            5718..8054
                     /locus_tag="AC16YU_11170"
     CDS             5718..8054
                     /locus_tag="AC16YU_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HIR6065830.1"
                     /translation="MFQLELFGSPSLAGDDGAWLTGRAVQRHRIALLALLSRAPAQRL
                     SRDRLIFYLWPESDAEPGRNLLNVSTYVLRKALGENAILSAGDDLRLNTEVVQTDVVE
                     FEAALRRADHARVVALYRGPFLDGFFLSETPEFEHWADRERELLAGGYGQAAEALAVA
                     AEAEGDTSKAAEWWKARAAHDPYDSRVALRLMQALEASGNRAGALQHAAMHQRLLQEE
                     FGMGPAPEIAALAERLRSETAAFSRRTVATGEQEREPPAPPDAEQNVPAPVDPRPAPP
                     TYASTPAVVEGRGSRRRMWPGAATVLAVISFVVIVWVVRGRDAEPERSIVVLPFISMS
                     PDSDNEYFSDGLTEEIITRLSAVPDLKVISRTSAMHYRGRAMPLRQIADELNVTHVLE
                     GSVRRSDGRVRISAQLIEAQTDEHLWAENYEYDLGDLFRVQEEIAREVAQALDVTLGE
                     RERMLLARKGTGDPEAYELYRRGRYFWNSRTKEGHEQAIGYYRRAIERDSSYADAYAG
                     LADVYLTAYQLNVFQMPETEAYSRLKREAERALALDDQSADAHRSFAIALWWQRNWPG
                     AEREFRRALELEPGNATARSWYSLLLGGWGRFDEALRESRRAYDLDPFAPAVGSNYAR
                     QCYLARDYDCAIEVYRKTLEVSPSWANGYRGLGLAYAQTGMLEEAMLAVQEAVALRPD
                     RTDYLADLAYVHALAGSPAQAREFLDRAKEQPWEGFNAARAHVAMDEPDSAFAWFERS
                     NWQWSHRANLYDPALDPLRSDPRFAQWLVRIRREMGIE"
     gene            complement(8108..>10158)
                     /gene="recG"
                     /locus_tag="AC16YU_11175"
     CDS             complement(8108..>10158)
                     /gene="recG"
                     /locus_tag="AC16YU_11175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6065831.1"
                     /translation="PRRAQAFAEAGVTTVRDLLQVYPRRYLDRSTVTPIREIRADSGT
                     VTVVGTVRACGVVPGRARRRFELLLEDESGGRIKCVWFRGVGWIPKAFKVGDRVAVHG
                     KPQAYAGAISLPHPDFDRLDDEGPALETGRIIPLYPGGAAFERAGITSRSMRRILYEL
                     IREHGLKLEDPLPEAMRRELGLLDGRVALRAVHFPKNRTELEQARARLKFDELFWIQL
                     MLARLRRKREAAPGPVLGDLGPLGRRFVSEVLPFVLTGDQEAAIRDVVRDASTGRQMN
                     RLVQGDVGSGKTVVGIAAMLLAIDHGYQAAFMAPTEILAEQHHAVLRQYLDPLGVEVR
                     LLVGGQRKAYRDEALAELADGRAHVAVGTHAVIQEGVAFHRLGLAVVDEQHRFGVMQR
                     ARLFSKGEHPHMLLMTATPIPRSLAMTLYGDLDVTLIREKPAGRRPIETWLRTEKKRE
                     EVFEFVKGQLREGRQCYVVYPLVEESEKLDLKDAESGYEALKQAFRPYTVDLVHGRMS
                     GWEKDDVMSRFKAGKTDLLVSTTVIEVGVDVPNATVMLVEHAERFGLSQLHQLRGRVG
                     RGAEQSWCILMADFKRSKESEERLEAMISTDDGFEISEIDLRMRGAGDFFGTRQSGMP
                     DLKIADITRDLEILTAARDAASSLVDRDPELQEPEHQSLREHFERYYADGSLGLARVG
                     "
BASE COUNT         1496 a         3563 c         3538 g         1561 t
ORIGIN      
        1 aaggtcgacc cggtagcggt agtctccgtg gccggttatg gcttcgttca tggttggcgt
       61 gatggattcc gggtatccga gtatacgccc gtgctgaacc gccccggctt tcccagagac
      121 tccaacccct caaaacccca actgccaggt gtccgattta tcggggggag gtctgtattc
      181 cggtgtttcc ggccaccgat tccggactct gtccggcctg gcaagttgga cagcccgcca
      241 ctttgtctgt cgggcacccg tcgggaaacc ccctccccca tgagaaaagc cctccccaac
      301 cccctgccca gcagggagcc atggagaggg cttcgcttcg taccgcgtac gggatttgaa
      361 cccgtgttac cggcgtgaga ggccggcgtc ctggacccct agacgaacgc ggcaggtgat
      421 cgagtccgaa taaactacgg cgtccgcggc caggtttccg gcctccagac ggccttcctc
      481 agatttccgc cgttcccgta ccggcgcatc cacgccgccc gctgcaccga cccgcgcccc
      541 gccctccgcc agcacccgca cgaccctccc ccctcacggc accacccaca gcggcgccag
      601 gaactccgcc gtccgcgtga tgctgccgtc cagcgtcagc gcattgtggc cgccgccctg
      661 gtacaggtag ctctcgaact ccgcggccgg cttgccggcg cgctgcatgg cgcggatcag
      721 cgactcggcc tggctcacgt ccacgaccgg gtcggcggtg ccgtggtgca cctggaccgg
      781 gggcatcgag tccacgaaga ggaccgcgga gcgccgcacg agctcggggc gcacgtcgcc
      841 gatcgagagc atggcgtccg cgagcggctg gatgaactcg ctgtccaggt agctgacgcc
      901 cggaaggtcg ggcgggttgc cgtcgagggc ctcctccacg atcgcctgca cgtaggagcc
      961 gaagaagtcg gtcgggccga agaattccac gacccggtcc acccgctcgt cgcgggcgcc
     1021 catgagcagc gccacgccgc cgccgcggct caggcccagc gccgcgacgc gctccggatc
     1081 ggccgcgggg gccacgtcga aggccgcgct caggagggcc atggcgtcgt ccacgtcgcg
     1141 gtcccagggg ctcggcgggc cctcggaggt gaacgtacgg ccgtcgaagc ggagcgtctc
     1201 gctccggaac gagggaacga ccatcaccat gtcgccgatc gccgacccga tggtctcgat
     1261 cgcgggcagg acctcgtcgt cgagcgacgt gccgccgtcg ccgccgtgca ggtacacgag
     1321 gaccggcagc gagccgggcg cggcgccgtc cggggtgacg atcccgccgt agtgcttcgt
     1381 gccctcgacc acgtgcgaga cgacctgcac caggaagcgc gcgtcgccgg tgccgacgct
     1441 cttgatgtgc tctatccgca ccgtccgcgc ggagacgtcg cgcagcgccc actcggcctc
     1501 gatggccgaa atctccgaga gcgacggcgt cgcaaagagc acgtccaggt tcacgccgcc
     1561 cacgatccgc tcggtttcgg tgtagtcgca ggacgagagg gcgaggacga gggcgatcgc
     1621 cgggaggagt cgcagcaggc gcatggaagt ggtcgaatgg gatggcggcc ttacgcgggg
     1681 acggggcgca ggttcggggc gcgagggccc cgcgcctggg ctcgcctcga ttcgatccgg
     1741 cggcggcgcc ctcccggccc gggcagcagc gtgcgggcct cccccgttcc cacccgcggg
     1801 cgccgagaaa ccgcggccgg ggccggcccg ttccaagcgg gcaccccgaa cgggcccccg
     1861 gtttcatggc cgatcccatc tacctggact acaacgcgac cacccccgtc gacccgcggg
     1921 tgctggagca catgctgccg tacttcacgg agcgcttcgg caatccgtcc tcgaagcggc
     1981 acgcctacgg ctgggcggcg gacgaggccg tcgagcgcgc ccgcgagcag gtcgcgggcc
     2041 tcctcggggc cgagccgcac gagatcctct tcacctccgg cgccaccgag gccgcgaacc
     2101 tcggcatcaa gggctatgcc gagaccatgg cgagccgggg ccgccacgtt gtgacgtgcg
     2161 cgaccgagca caaggccgtc ctcgaggcgt gccgttcgct cgcgcgccgc ggattcgagg
     2221 tcaccgtact gcccgtcgac gcggacggcc tcctgcagcc ggacgtggtg gaggaggcgc
     2281 tccgcgacga cacggtgctc gtcgccgtga tgtgggcgaa caacgagacg ggcgtcgtcc
     2341 accccgtggc ccggatcgcc gagcgcgtgc acgcgcgcgg catcgcgctc ttctgcgacg
     2401 ccacgcaggc cgtgggcaag gtgccggtcg acgccgggca cgtggacctc ctcgcctgct
     2461 cgagccacaa gttctacgga ccgaagggca cgggcgcgct gttcgtcgcg cggcgcgaac
     2521 gccgcctccg cgtcgcgccc cagatcgagg gcgggggcca ggagagcggc ctgcgcggcg
     2581 gcaccctcaa cgtgccgggc atcgtgggca tcggcgccgc ggccgagatc gcgggcgagg
     2641 acatggaggc ggacggctcg cgcctcgagg ccctgcggga cgaaatcgag cgctcggtcg
     2701 ggcgcggttt tcccgacctg cgcgtcaacg gccggggcgc accgcgtctg ccgaacacga
     2761 gcagcatgac cttccacggc ctttccgcgg ccgacctgat cgcgggcacg cgcgacctcg
     2821 cgctgtccac cggatcggcc tgctcgtcgg gaacgggcaa accgagccac gtcctccagg
     2881 cgatggggct ttcggacgcg gacggtgcgg ccaccatccg cctgagcgtg gggcggttca
     2941 cgaccgcgga cgaagcgtcg gccgccgcgg agcgcctcgc ctcggtcgcc tcctccctcc
     3001 tcgagcggac gcccgcctga ccgaaccgaa gcccgagcct ggccgatccg aatcccccgc
     3061 ccgaccgacc cgaaacccaa gcctgaccga gccatggaac ccatcgtgaa ccgggtggcg
     3121 cagagcggca tcgaggtcct ggacctctcg atgttcgagc ccgacggcga ccccgtcgag
     3181 ctggacatcg cgccgttcct ggtcgagggg ctcatcctgc gcgagaagcc gttccgggaa
     3241 gcggtcgccg cgttcgactt ctcgccgttc gcgggccgcc tggtcgccgt ccggtgctcc
     3301 gcggacgcga tcgttccgac ctgggcctgg atgctcgtcg cgacgcgcct ggacgggatc
     3361 gcgacggcgg tcacggtcgg atcgcccgcc gacgcgatgc gcgagcactt cgcgcgggcg
     3421 gtcgccgcgt tcgactggtc gcgctacgaa gaccggacgg tcgtcgtcaa gggctgcggc
     3481 tcgggccgcg tgcccgaggc ggcgtacgtg atggcgacga cggccctgca gcgcgtcgcc
     3541 cggaaggtga tgttcggcga gccttgctcg acggtgccgc tgtggcgccg cgggtcgggc
     3601 ggccgagggt cctgagcgct gatgcagccg gcgacggtcc ccgcggcgga ggtttcggtc
     3661 ccgggcggac gcctggggca gcgcctgttc gcgctgtacc gccgccacga gcggatcgcg
     3721 cccgcgctgt tcttcttcgc gggcatcggc tgggacgcgg ccacggtgtt ccgcatcgac
     3781 gccctgatcg acaacgtgct cgtcgccctg tacctggccg cgctcggcgt gctcgtcacc
     3841 gcctccgcgc tggacggggc cgggcagttg cgctcgcgct tcctggcccg ctggcgcccg
     3901 tggtttccgg ccgcaatgca gttcttcctc ggggcgctct tcagcgtctt cgtcttcttc
     3961 tactcgcaga gcgcgtccct gagccagacg tcggtgtacc tggggctgct cgtcgcgctc
     4021 ctcgtcgcga acgagttcct gcaccgccgc atgctggacc tgcacctggg cctcgggctg
     4081 tattacctgg cgctcgcgag cttcctggtg tacttcgtcc ccgtcgtgac cgggaggatg
     4141 gggacggcga tgttcctgct cggcaccctg gcggcggccg gggccgtgct cgccatcgtc
     4201 tcgctgcttc ggcggcgcgg ggtgttcgca tccgcccgcc aggcggcgtc ggtgagcgca
     4261 ctcgtcgtgg tgctcttcgg attccaccag gcggcgtacc acctgaactg gattccgccc
     4321 gtcccgatcg cgctccggga tgcggggatc taccgggagg tcgtacgcga gggcgacacg
     4381 taccggatgc gcttcgcacc gcagccctgg tggatgttct ggcggcggga cgagcggcac
     4441 ctcgactggc ggccgggcga gcccgtctac tgcttcaccg cggtcttcgc gcccacgcgg
     4501 ctcgagagcc ggatcgtcca cgtgtgggag cacctggacg aggcgacggg cgagtgggcc
     4561 gagtccgacc ggatcggcta cgacatcgtg ggcgggcgcg actacgggta ccggggctac
     4621 accgtcaagc gcaccatgcg gcccggccgc tggcgggtgc gcgtggagac ctccggcgga
     4681 cggctcctga gccggatccc cttcaccgtg cgcgaggcgg agccggccga cacggcctgg
     4741 acgtggatca cggcgcggtg aggaatggcc cggaccggcc gaaccgatcc gggccatccc
     4801 gggaatcact accgagccct actacagctg cacttgcatg cgaccgtcct tgcgcgcagc
     4861 tctaattgca caatgccgcg gacgggaact acagcgagca gtcgcccggg gtatcgagga
     4921 gctcggtggg ccccttgccc ggcggaatgt cgatgaaggt cgcgtagacg acgacctcgg
     4981 cctccgtgtc gttgacggcg acgtgtacgt tcccctgtcc cgggtccagg aatgcctttc
     5041 cggccgcgta caggcgcggc acgcagtcgc tttcgttgac gatcgtaagc tcacccgcgg
     5101 atatggtcac gatcgcaggc cccggatggc tgtgccagcc gacgaacccg tgtggagcga
     5161 aggtgagttt cgcgacgacg acgtctttcg agtcgtgtat gtctgcgact gtcgtgcccg
     5221 gactgcctgc caggttgact cggaacagtc cgtcgatctt gtccgggaag ccgccgcgtg
     5281 ccagcggctc caccgtgacg ccctggaccc cgatgtagtc tgccgaggag agcaacgccg
     5341 acgctgcggc cttccccgcg aaaaaggtcg tctgttgagc gttcaattcg ggttcgtcgc
     5401 cgagcgagcc ctgctggtcg catccgaaca gcattacggt cacggccgca gcgacgatga
     5461 tgccggacgt cggttggaaa gaccgccggc cgttccgttc ctctcgtttc atggttctga
     5521 cctccaggtg tgttttgtgt ggacgccgag gatcattaat cggattttca tggtctacct
     5581 cgttcaggcg aggcaggtag cacggacaac gcggtgcgct tctgatgccc actccaaaag
     5641 cggtcgcggg gagatgctaa gcgcagggaa tgaacgcgaa gtgtcgctgc ggtgaacgcc
     5701 cgataaaccg gttcctgatg ttccaactcg agctcttcgg gagtccatcc cttgcgggtg
     5761 acgacggagc ctggctcacc ggtcgagccg tccagcgtca ccgcatcgcc ctgctggccc
     5821 tgctctcgcg ggcgcccgcc cagcgattga gccgggaccg gttgattttc tacctctggc
     5881 cggagagcga tgcggagccg gggcgcaacc tcctgaatgt ctcgacatac gtgctccgga
     5941 aggcgctggg cgagaacgca atcctgtcgg cgggtgacga cctgcggctg aataccgagg
     6001 tggtgcagac ggatgtggtg gagttcgagg cagccctccg gcgggcggat catgcacgcg
     6061 tggtggcgct ctacaggggt cccttcctgg acggcttctt tctgagcgag acgccggaat
     6121 tcgaacattg ggcagaccgg gagcgcgagc tgctcgccgg cggatatggc caggcagcgg
     6181 aagcccttgc cgtggcggcc gaggccgagg gggacacttc gaaggcggcg gagtggtgga
     6241 aggcgcgggc ggcgcacgat ccctacgact cacgcgtggc gctccggctc atgcaggcgc
     6301 tggaagcgag cggaaaccgt gctggagcgc tccaacacgc cgccatgcac cagcgcctgc
     6361 tccaggagga attcgggatg ggcccagctc ccgagatcgc tgccctggcg gagcggcttc
     6421 ggagcgagac cgcggccttc agccggcgga cagtggccac gggagaacag gagagggaac
     6481 ctcctgcccc accggatgcg gagcagaatg tcccggcccc cgtggaccct cgccccgcac
     6541 cgccgacgta cgcgtccacg ccggccgtgg ttgagggccg ggggtcacgc cggaggatgt
     6601 ggccgggcgc cgcgacggtg ctcgccgtca tctccttcgt ggtcatcgtc tgggtagttc
     6661 gggggcgcga tgccgagccg gagcgctcga tcgtcgtact gcccttcatc agcatgagcc
     6721 cggacagcga caacgaatac ttcagcgacg ggctcaccga ggagatcatc acccgtctgt
     6781 cggccgtccc tgatttgaag gtgatctcgc ggacctcggc gatgcactac aggggacgcg
     6841 caatgcctct ccggcagatc gcggatgagc tgaatgtcac tcacgtcctc gagggcagcg
     6901 tgcggcggag tgacggccgc gtgcggatct cggcgcagct catcgaagcc cagaccgacg
     6961 agcacctctg ggcggagaat tacgagtatg acctggggga cctcttccgg gtgcaggaag
     7021 agattgcacg ggaggttgcg caggctctcg acgtgactct gggggagcgg gagcggatgc
     7081 tgctggccag gaaaggcacc ggcgatccgg aagcatacga gctctaccgc aggggccgct
     7141 atttctggaa ctcgcggacg aaggagggtc acgagcaggc gatcgggtac taccggcggg
     7201 cgatcgagcg cgactcgagc tatgcggatg cgtacgccgg gctggcggac gtctacctca
     7261 ccgcctacca gctcaacgtg ttccagatgc cggaaacgga ggcatactcc cggctcaagc
     7321 gggaggcgga acgggcgctc gccctcgacg accagtcggc cgatgcccac cggtcgttcg
     7381 cgatcgccct gtggtggcag cggaactggc cgggcgcgga gcgcgaattc cgccgggccc
     7441 tcgagctgga accgggtaat gcgaccgcgc gcagttggta ttcactgctg ctcggcgggt
     7501 ggggacggtt tgacgaagcg ctccgggaga gccgccgcgc ctatgatctg gacccgttcg
     7561 cgcccgcggt cggcagcaac tacgcccggc agtgctatct cgcccgcgac tatgactgcg
     7621 ccatcgaagt gtaccgcaag acactcgagg tcagcccctc ctgggcgaac ggctaccggg
     7681 ggcttggcct ggcgtatgcg cagacgggca tgctcgagga ggcgatgctc gcggttcagg
     7741 aggccgtcgc cctgagaccg gaccgcacgg actacctggc ggatctcgcc tacgtgcatg
     7801 cgcttgccgg aagcccggcg caggcccggg agttcctgga ccgggccaag gagcagccgt
     7861 gggaaggatt caacgccgcg cgggcgcacg tggcgatgga cgagcccgac agcgccttcg
     7921 cctggttcga gcgcagcaac tggcagtggt cgcaccgggc caacctgtac gaccccgcgc
     7981 tggatcccct gcgctccgac ccgcgctttg cgcagtggtt ggtgcggata cggcgggaga
     8041 tggggatcga atgaacaccg gccggtgacg tggatcagca gacgccccgg tcgcctcccc
     8101 gctcgcctca ccccacccgc gccagcccca ggctgccgtc cgcgtagtac cgctcgaagt
     8161 gctcccggag cgactggtgc tcgggctcct ggagctccgg gtcccggtcc acgaggctgg
     8221 acgccgcatc gcgcgccgcg gtgaggatct ccaggtcgcg cgtgatgtcc gcgatcttca
     8281 ggtcgggcat cccgctctgg cgcgtcccga agaagtcgcc agctccgcgc atccgcaggt
     8341 cgatctccga gatctcgaaa ccgtcgtcgg tcgagatcat ggcctcgagg cgctcctcgc
     8401 tctccttcga gcgcttgaag tccgccatca ggatgcacca cgactgctcg gcgccgcggc
     8461 ccacgcggcc gcgcagctgg tgcagctggc tcagcccgaa gcgctcggca tgctcgacga
     8521 gcatgaccgt ggcgttcggc acgtccacgc ccacctcgat cacggtcgtg gacacgagga
     8581 ggtcggtctt gccggccttg aaccggctca tcacgtcgtc cttctcccag cccgacatgc
     8641 gcccgtgcac gaggtccacc gtgtacggcc ggaaggcctg cttcagggcc tcgtagcccg
     8701 actcggcgtc cttcaggtcc agcttctcgg actcctcgac cagcgggtac acgacgtagc
     8761 actgcctccc ttcccgaagc tgccccttca cgaactcgaa gacctcctcg cgcttcttct
     8821 ccgtgcgcag ccaggtctcg atggggcgcc gtccggcggg tttctcgcgg atcagcgtca
     8881 cgtccaggtc gccgtagagc gtcatggcca gcgagcgcgg gatcggggtc gcggtcatga
     8941 gcagcatgtg cgggtgctcg cccttcgaga acaggcgcgc gcgctgcatg accccgaagc
     9001 ggtgctgctc gtcgaccacc gcgaggccca accggtggaa ggccacgccc tcctggatca
     9061 cggcgtgcgt gccgacggcc acgtgggcgc ggccgtcggc gagctccgcg agcgcctcgt
     9121 cccggtacgc cttgcgctgc ccgccaacga gcaggcgcac ctcgacgccg agcgggtcca
     9181 ggtactgccg cagcacggcg tggtgctgct cggccaggat ctcggtcggc gccatgaacg
     9241 ccgcctggta gccgtggtcg atggcgagca gcatggccgc gatgcccacg accgtctttc
     9301 cgctgcccac gtcgccctgc accaggcggt tcatctggcg cccggtcgac gcgtcgcgga
     9361 ccacgtcgcg gatggccgcc tcctggtcgc ccgtcagcac gaagggcagc acctcggaca
     9421 cgaaccgccg ccccagcggc cccaggtccc cgagcaccgg ccccggcgcg gcctccctct
     9481 tcctgcgtaa gcgcgcgagc atgagctgga tccagaacag ctcgtcgaac ttgaggcgcg
     9541 cgcgcgcctg ctcgagctcc gtgcggttct tggggaaatg gacggccctg agcgccaccc
     9601 ggccgtccag gaggcccagc tcgcgccgca tcgcctcggg aagcggatcc tccagcttca
     9661 gcccgtgctc ccggatcagc tcgtacagga tgcggcgcat gctccggctc gtgatgccgg
     9721 cccgctcgaa ggccgcgccg ccgggataga gcgggatgat ccggccggtc tcgagcgccg
     9781 gaccctcgtc gtccagccgg tcgaaatccg gatggggcag gctgatcgcg ccggcatacg
     9841 cctgcggctt gccgtgcacg gccacgcggt cgcccacctt gaaggccttc gggatccatc
     9901 cgacgccccg gaaccagacg cacttgatgc gcccgcccga ctcgtcctcg agcaggagct
     9961 cgaaccggcg ccgggcgcgc ccgggcacca cgccgcacgc ccgaacggtg ccgacgaccg
    10021 tgaccgtccc cgagtcggcg cggatctcgc ggatcggcgt gacggtggag cggtccaggt
    10081 agcgccgggg gtacacctgc agcaggtcgc ggacggtcgt cacgccggcc tccgcgaagg
    10141 cctgggcccg gcggggac
//
LOCUS       DBNHQZ010000410         5009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3373914, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000410 DBNHQZ010000000
VERSION     DBNHQZ010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3373914"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16YU_11180"
     CDS             complement(<1..296)
                     /locus_tag="AC16YU_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HIR6065832.1"
                     /translation="MAQRVLACIGTKKGLFVAESSANRDDFELRGPFSPGVAVYSALI
                     DRRDFPRIFASSCNAWFGMNFLRSDDVGRSFEATKSAPTFPENDGRALANIWSL"
     gene            complement(299..574)
                     /locus_tag="AC16YU_11185"
     CDS             complement(299..574)
                     /locus_tag="AC16YU_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6065833.1"
                     /translation="MDVTISVPSVLRSHCGGRSSLALKAESVRISLTELKRSHPSLHA
                     RVCDETGAVRRHINIFVGSAHMRDLEGLDTPLAQGDVITILPAVSGG"
     gene            complement(753..3128)
                     /locus_tag="AC16YU_11190"
     CDS             complement(753..3128)
                     /locus_tag="AC16YU_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6065834.1"
                     /translation="MFRSHLSVAFRILVRHPGYSAINVLGLAVGLASTLLILLFVADE
                     LSYDAFQEDADRIVRITADVLDESGASRLESVLVDPGWAPLIDEGLPGVEAVVRLTPV
                     GPVLSSGDRRIACGDCYWVDADLFRVLTFDLVEGDPATALSEPSSLVLSRSRALALFG
                     EVDVVGRSVTVNGDREVTVTGVFEDLPHDTHLPADGFGTLSSFAWFDPTAWGSPNYAT
                     YVKLAPGTDPDELEAGLADLVARHRTPEDAARIRLGVQRIRDIHLHSHRVGELGVNGN
                     ASTVALLAAVAFFILAIACINFMNLSTARSSLRAREVGVRKATGAQRSQLVGQFLGES
                     VMLASAALVVAALLVQLLLPAFNAFTGKDVSLLSAGTLAVLVGVGLAAGVAAGSWPAF
                     FLSSFRPAEALRGENGNRSTGGRLRAALVVIQFTIAVVLAIGTMVVFRQLDFVRSRPL
                     GYDRENVLVLPVMWDRMERFGPIEDRLLANPDVVSLSQSNPVPGGRLHTSIEAVVEDQ
                     AAGRLESASLFAVWADDGFLETYGIDLLAGRNFALEFASDADTGFLLNALAAQRLGFD
                     RPESAVGSPLRVGGWRGRVLGVVEDVHFESLHQPIAPQVYFMDPRNFRAVSVRIRPGA
                     DVAEVAAFLEREWALHDPSAIFSYTVLDDRLRQLYDGERRLGRLIGVFAGLALLVTCL
                     GVFGLAAFAAERRTREIGIRKVFGAGVPSIVARLNRDILALVGAAIVIGVPVGVTVAY
                     RWLDTFAYGTGIPWWLLAAVAIGFVAIATATVSIHAVRAATANPVRALRYE"
     gene            complement(3166..>5009)
                     /locus_tag="AC16YU_11195"
     CDS             complement(3166..>5009)
                     /locus_tag="AC16YU_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6065835.1"
                     /translation="AISFENYPNPWLGGNNWGGRTSTYLRLGEGQTAAGLEEVAQSTV
                     HRLMAEYMDALRGNGYLAEADEAFTLHLQPLEEMHRDVVVWIPYEAAAHNPRYSLILA
                     TIGFMILLIACINFMTLSVGQSTTRAREVGVRKALGAGREQIMRQHWGESVALATLSI
                     AAGIGLAFLALPGFRSLTGSEVSFGSVPLVGAAAAIVLLIAVVGVIAGGYPAVVLSRF
                     VPVRVLKGDVDAPRQGLFTRSLVVLQFTISISLIACTVIMTRQLGFMLEKDLGFDEEF
                     VVAVDAQQVPGPEKEALLERLKLELASNPDILGVERTGYAFTRGHDRNTWRDANGNTR
                     GAYNYGVGYDYLEVMGMELAAGRFFSEDHPADPTSSIVVNQALVDEFGIEQPIGHVLT
                     NWLDFIYDESPTIIGVVRDFNFLPLHQKVPPLVMNMHPDYYSWMGAVLIKVRPGDVAG
                     ALSFIEASWNRAMPGKPFTYSFVDEDMAAQYGTERRWQRILAYSSMLAILIASMGLFG
                     LALLAVNRRTKEIGIRKVLGASVPRLSTLLTREFAVMVVVAAVIATPLSVLAMRKWLD
                     GFAYRIDLSPWVFVLATALALLIAVATVSLHAVRAALSNPVRSLRYE"
BASE COUNT          871 a         1723 c         1718 g          697 t
ORIGIN      
        1 tcgagggacc agatgttggc aagcgcccgg ccgtcgttct cggggaaggt gggcgccgac
       61 ttcgtcgcct cgaagctccg gcccacgtcg tcggagcgga ggaagttcat gccgaaccag
      121 gcattgcagc tcgacgcgaa gatccgcggg aaatcgcgcc ggtcgatgag tgccgagtac
      181 acggccaccc cgggcgagaa cgggccgcgg agctcgaagt cgtcgcggtt cgccgacgac
      241 tcggccacga agagaccctt cttggttccg atgcaggcca agacacgttg agccatggtc
      301 agcctcctga tactgcgggg agtatggtta tgacgtcgcc ctgcgcgagc ggcgtgtcaa
      361 gcccttccag gtcgcgcatg tgcgcgctgc ccacgaagat gttgatgtgg cgccgcaccg
      421 ctcccgtttc gtcgcagacg cgggcatgaa gcgacgggtg gctccgcttc agctcggtga
      481 gcgaaatccg aacgctctcg gccttcaatg cgaggctgga ccgccctccg cagtggctgc
      541 gcaggacgga cggcacgctg atggtcacgt ccatggtccg ggtgaggttc gtcgccgcgg
      601 ggcgatgccg acgaaccgcg ggggcgcggc gccgtcggct gccgggaatt tcccgaccgg
      661 gacgttcagc tgcaagcggt ccatcgcggg caggtcgatt tgccacccga acggcgcagc
      721 gccgattccg cgagcggcgc cgggcgcagc cctcactcgt accggagcgc ccggaccgga
      781 ttcgccgtgg cggcgcggac ggcgtggatg ctcacggttg ctgtggcgat cgcgacgaag
      841 ccgatcgcga ccgcggcgag gagccaccac ggaatccccg tgccgtaggc gaacgtgtcc
      901 agccagcggt aggcgaccgt cacgccgacg ggcacgccga tcacgatcgc cgcgccgacc
      961 agtgccagga tgtcccggtt cagccgcgcc acgatggagg gcactccggc cccgaacacc
     1021 ttgcggatgc cgatctcgcg tgtccggcgc tcggccgcga acgcggccag cccgaacacc
     1081 cccaggcagg tcacgagcag ggcaagcccg gcaaacacgc cgatcagccg gccgaggcgt
     1141 cgctcgccgt cgtagagctg gcgcagccgg tcgtccagca cggtgtacga gaagatggcg
     1201 gatggatcgt gcagcgccca ctcccgttcc aggaaggctg ccacttccgc cacgtcggcg
     1261 cccgggcgaa tcctcaccga gaccgcgcgg aagttgcgcg gatccatgaa gtagacctgc
     1321 ggcgcgatcg gctggtggag cgattcgaaa tgtacgtcct ccacgacgcc caggacccgg
     1381 cctcgccagc cgccgacgcg cagcggcgat ccgacggcgg actccggacg atcgaagccc
     1441 agccgctgcg cggcgagcgc gttgagcagg aagcccgtgt cggcgtccga tgcgaactcg
     1501 agggcgaaat tgcgaccggc gaggaggtcg atgccgtacg tctccaggaa tccgtcgtcc
     1561 gcccagacag cgaagaggga tgcggactcg agccggccgg cggcctggtc ctcgacgacg
     1621 gcctcgatcg acgtgtgcag ccggccgccc gggacagggt tcgactgcga caggctcacg
     1681 acgtccgggt tcgccagcag ccgatcctcg atcgggccga accgctccat ccggtcccac
     1741 atcaccggca ggaccagcac gttctcgcgg tcgtatccga gcggccggga ccggacgaag
     1801 tcgagctgcc ggaagacgac catcgttccg atcgccagga cgaccgcgat cgtgaactgg
     1861 atgacgacca gggccgcccg cagcctcccc cccgtcgacc ggtttccgtt ctccccgcgc
     1921 agcgcttccg ccggccggaa ggacgacagg aagaaggcgg gccacgaccc ggcggccacg
     1981 cccgccgcca ggccgacgcc cacgaggacc gcgagcgtgc cggccgacag gaggctcacg
     2041 tccttccccg tgaacgcgtt gaacgcaggc aggagcagct gcaccaggag cgccgcgacg
     2101 acgagcgccg cggaggcgag catcaccgac tcgccaagga actgccccac gagctgcgac
     2161 cgctgggccc cggtcgcctt gcggacgccc acctcgcgcg cgcgcagcga agagcgcgcc
     2221 gtggacaggt tcatgaaatt gatgcacgcg atcgcgagga tgaagaaggc cacggccgcg
     2281 agcagggcca ccgtcgaggc gttcccgttc acacccagct cgcccacgcg gtgcgagtgc
     2341 aggtggatgt cccggatccg ctgcaccccc aggcggatgc gtgccgcgtc ctccggagtg
     2401 cggtgccgcg ccacgaggtc cgcaagcccg gcctcgagct cgtcggggtc ggtccccggt
     2461 gccagcttca cgtaggtcgc gtagttgggc gagccccagg cggtcgggtc gaaccacgcg
     2521 aacgaggaca gcgtcccgaa tccgtccgcc ggcaggtgcg tgtcgtgcgg caggtcctcg
     2581 aagaccccgg tgacggtaac ctcccggtcg ccgttcacgg tgacggaccg gccgacgacg
     2641 tccacctcgc cgaagagcgc gagggcacgc gatcggctca gcacgagcga gctcggctcc
     2701 gagagggccg tcgcgggatc gccctctaca aggtcgaacg tcagcacccg gaagagatcc
     2761 gcgtccaccc agtagcagtc cccgcaggcg atcctccggt ccccgctcga gagcacgggg
     2821 cccaccggag tgaggcgcac gacggcttcc accccgggaa gcccctcgtc gatgagcggt
     2881 gcccatcccg gatccacgag cacgctctcg agacggctcg cgcccgattc atcgagaacg
     2941 tccgcggtga tccgcacgat acggtcggcg tcttcctgga acgcgtcgta cgagagctcg
     3001 tccgcgacga aaagcaggat gagcagcgtc gacgccagcc ccacggccag gccgaggacg
     3061 ttgatcgccg agtagcccgg gtgccggacc aggatccgga acgccacgga cagatggctt
     3121 ctaaacatgg ctgattcctc tgcaggcggc cggtcgcccg acggatcact cgtagcgcag
     3181 gctgcggacg gggttggaga gcgccgctct cacggcgtgg agggacaccg tggccacggc
     3241 gatcaggagg gcgagcgcgg tggcgagcac gaagacccat gggctcaggt cgatccggta
     3301 ggcgaagccg tcgagccatt tccgcatggc cagcacggaa aggggcgtgg cgatgacggc
     3361 ggccacgacg accatcacgg cgaactcccg cgtcagcagg gtggacagcc tgggcaccga
     3421 cgccccgagc accttccgga tgccgatctc ctttgtgcgt cggttcaccg ccagtagcgc
     3481 gagcccgaaa aggcccatcg acgcgataag gatggccagc atcgaggagt acgccaggat
     3541 ccgctgccag cgccgctccg tcccgtactg ggccgccatg tcctcgtcga cgaaggagta
     3601 ggtgaacggc ttgcccggca tcgcccggtt ccaggaggcc tcgatgaagg acagagcgcc
     3661 ggcgacgtcg cccggccgga ccttgatgag gacggcgccc atccagctgt agtagtccgg
     3721 atgcatgttc atgacgaggg gcgggacctt ctggtgcagc ggcaggaaat tgaaatccct
     3781 gaccacgccg atgatggtcg gcgactcgtc gtagatgaaa tcgagccagt tcgtgagcac
     3841 gtggccgatc ggctgctcga tgccgaattc gtcgacgagc gcctggttca ccacgatgga
     3901 cgaggtcggg tcggccggat ggtcctccga gaagaagcgc ccggcggcga gttccatgcc
     3961 catcacctcc aggtagtcgt accctacgcc gtagttgtag gcaccgcgcg tgttgccgtt
     4021 ggcgtcccgc cacgtgttgc ggtcgtgccc gcgcgtgaac gcgtaccccg tgcgctcgac
     4081 gccgaggatg tcgggattgg acgcgagctc cagcttgagc cgctccagta gtgcctcctt
     4141 ctcgggcccg gggacctgct gggcatcgac ggccacgacg aactcctcgt cgaagccgag
     4201 atccttctcc agcatgaagc cgagctgccg cgtcatgatg accgtgcagg cgatcaggct
     4261 gatcgatatc gtgaactgca gcacgaccag cgatcgcgtg aagaggccct gtcgcggcgc
     4321 gtccacgtcc cctttcagca cccggaccgg aacgaagcgc gagagcacga ccgccggata
     4381 gccgcccgcg atgaccccga cgacggcgat caggagaacg atcgccgccg cggctccgac
     4441 gaggggcacg ctgccgaacg agacctccga gccggtcagg ctgcggaagc cgggaagcgc
     4501 gaggaaggcg agcccgattc ccgcggcaat cgacagggtc gcgagcgcga cggactcgcc
     4561 ccagtgctgc cgcatgatct gttcgcgccc ggcgccgagc gccttgcgga cgcccacctc
     4621 cctcgcacgc gtcgtggact gtccgacgga cagcgtcatg aagttgatgc aggcgatgag
     4681 caggatcatg aagcctatgg tcgccaggat cagcgagtac ctggggttgt gcgcggcggc
     4741 ctcgtacgga atccatacga cgacgtcccg gtgcatctcc tcaagcggct gcaggtgcag
     4801 cgtgaacgcc tcgtccgctt cggccaggta gccattgccg cgcagggcgt ccatgtactc
     4861 ggccatcagc cggtgcaccg tcgactgggc gacttcctcc aggccggcag ccgtctgccc
     4921 ttcgcccagc cggagatagg tgctcgtccg gccaccccag ttgttgcccc ccagccacgg
     4981 attcgggtaa ttctcgaacg aaatggccg
//
LOCUS       DBNHQZ010000411         6445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3374043, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000411 DBNHQZ010000000
VERSION     DBNHQZ010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3374043"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="AC16YU_11200"
     CDS             complement(<1..1483)
                     /locus_tag="AC16YU_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR6065836.1"
                     /translation="MKRLSAALALLLLVAGPALARQDSATRDRLLMDFGWRFTTGDPA
                     GAEAPGFDDSAWRMVDLPHDWSIEGPYDEQAATTGRGGYLPTGVGWYRRTFTVDPVDR
                     RVWVEFDGVHQNSDVWVNGAHVGHRPYGYIGFMHDVTDQVRTGANVVAVRVDNSKQPN
                     SRWYTGSGIYRHAWLSLVPPLHIAHWGTTVTTPSVDSAAATVSVRTRIVNEGPETRRV
                     RLWTWLTDDRWGRRVGGDEAPVELAPGAWADIEQSFQVLRPALWSPDTPSMYTLHQDL
                     QGEGGGDRVETIVGIRSIAFDADRGFLLNGQQVKMRGVNLHHDGGPVGAAVPEGVWER
                     RFRILKEMGANAIRTAHNPMAPEFMDLADRMGFLVMNEAFDEWTHGKVEYGYHVYFDE
                     WSERDLTDFIRRDRNHPSVVLWSLGNEIGEQHAPGGEDVLRRLREIAKREDPTRPITT
                     GNDHIAADDGATTEGFRELLDVVGYNYVDRWHERRELYAWPGRQ"
     gene            complement(1521..4109)
                     /locus_tag="AC16YU_11205"
     CDS             complement(1521..4109)
                     /locus_tag="AC16YU_11205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR6065837.1"
                     /translation="MSVRFPRMLGLVLVAGASALPIHAQPGPPVSVDDRGVMRWTASG
                     EEVTLFGVNYTLPHAFAYRALGYVGADRKQAIDADVNHLARLGLDAFRIHVWDREISD
                     RDGNLVENDHLDLLDYLLARLEEHGVKSILTPIAWWGTGYPERDQESTGFSNFYGKGE
                     MAVDSVARVAQRNYLEQFIAHVNPYTGKSYRDDPDIIAVEIFNEPSHPGGPEETTRFI
                     DALADALRGAGFAKPIFYNISQGYGERHGEAVCAARIQGVSCQWYPTGLVRGSTIRGN
                     MLPNVDRYPTPFTETPDCADKALMVYEFDAADVAGSYMYPAMARAFRGAGFQFAAQFA
                     YDPLAIAWSNTEYQTHFLNLVYAPGKAISFMIAGEVFRQVPRGSVFGAYPESATFGPF
                     RVSYEEDLSEMAAETVFLHSNSTGTAPPAPDRLLRLAGVGSSPVVAYEGTGAYFLDRE
                     SGGVWRLEVYPDVVWIDDPFERPSLDRALARVRWGTWPMRIDLPDLGPGFTVTPANEG
                     NDHRPAVDGTTFDIRPGVYLVTRSGIEVPDAPADRRPFAAPGANAGEPELVHEPPVEL
                     AEGSPFTVRARIVSDSPVDSALVLVAGQGGFGRFGRVRPPRLERASGYDFKATVPAER
                     VRGGLLDYRIVVYRGGSATTFPGAVEGQPSDWDFVSDAVYRVPVVGAGVPVVLFDARR
                     DMDRMLYPNPWGYVRFRTSLVGGTLPGSLALQAVATNLEPEPHHLAVRTWLPAEHLRG
                     ATTLGSDAVLRVLGRATESETESIQVALIERNGTAWAVSVPLTGSWQDVEVPLTSLRR
                     VPLALLPRPYPGFLPYTLEAATGSAAIDLANLDGLQIVIGGDAVPAEERSAARGFEIE
                     RILLIQ"
     gene            complement(4164..5072)
                     /locus_tag="AC16YU_11210"
     CDS             complement(4164..5072)
                     /locus_tag="AC16YU_11210"
                     /inference="COORDINATES: protein motif:HMM:NF046981.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR6065838.1"
                     /translation="MRNPVRSMLLLVVGVALALPTALLAQETITLVNPSFETAGGTTP
                     CTRSTNFNTGVPGWSTDTDPEDSGVSDNANATDGVCAAWLFNNDPEIWQLTDHVIAAG
                     ETYTLEADARSSWQTNLFEVRLYYDVAGTRTIVGTAQHDFEGISEPMVTLTLAAAADD
                     MGLVAIGNRLGVAVKNTTTEVDAWAEVDNVRLTVSGGTAVEDGTVPAGFTLAQNYPNP
                     FNPGTTIAYELQAAGSVRLSIHDTLGRTVRTLDEAVQAPGTHAAAWDGLLEDGTPATT
                     GIYLYRLTMQNGLSTTSRARAMLLLH"
     gene            complement(5268..>6445)
                     /locus_tag="AC16YU_11215"
     CDS             complement(5268..>6445)
                     /locus_tag="AC16YU_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HIR6065839.1"
                     /translation="FDNFDWGPNTFNGGNGPTYAKIRYVARATDVIDKIQKSEADVPD
                     AVKAKYVAEAKALRAWLMFVIYDFFGPVNAKLDPETLYDTEITPRMTKTDYVEAMETD
                     LLEAIPALPDMYNGDVANWGRVSKGAARMVLLRLYMHEHEWAKAEAVARDIMGMGYGL
                     EPYYPDVFNNEQNMEIIYAVPVNESAPNWYIQEIIPSNFGSSGDITREPGWYGFWMPW
                     DYYDKYDADDERQDALLSSYVTRTGQTRDRNSGMRGAVPLKFTNISGTSSNGVTFDVP
                     VFRYAEVLLSLAEAINEQVGPAEAYEYANLVRERAELEGFSGMNQDQFRDALLDERAR
                     EFFGEGLRRMDLIRHGRFIQVARDAGITNAQPHHVLFPIPQSVIQEGGGIIEQNPGY"
BASE COUNT          949 a         2223 c         2229 g         1044 t
ORIGIN      
        1 cctgccggcc cggccacgcg tacagctcgc ggcgctcgtg ccaccggtcc acgtagttgt
       61 atccgacgac atccaggagc tcgcggaagc cctctgtcgt cgcaccgtcg tcggccgcga
      121 tgtggtcgtt gccggtggtg atggggcgcg tcggatcctc ccgcttcgcg atctcgcgca
      181 ggcgccgcag gacgtcctca ccgccgggcg cgtgctgctc tccgatctcg ttgcccaggc
      241 tccagaggac gaccgacgga tggttgcggt cgcggcggat gaaatccgtg aggtcgcgct
      301 cggaccactc gtcgaagtac acgtggtagc cgtactccac cttcccgtgc gtccactcgt
      361 cgaaggcctc gttcatcacg aggaagccca tgcggtcggc caggtccatg aactccggcg
      421 ccatcgggtt gtgggcggtc cggatggcgt tcgcgcccat ttccttcagg atcctgaagc
      481 ggcgctccca gacgccctcg ggcacggccg ctccgaccgg cccgccgtcg tggtggaggt
      541 tcacgccgcg catcttgacc tgctgcccgt tcaggaggaa gccgcgatcg gcgtcgaagg
      601 cgatcgagcg gatgccgact atcgtttcca cgcggtcgcc gccgccctcc ccctggaggt
      661 cctggtgcag ggtatacatc gacggcgtgt cgggggacca gagcgccggc cgcaggacct
      721 ggaagctctg ctcgatgtcg gcccacgcgc ccggggcgag ctcgacggga gcctcgtctc
      781 cccccacccg cctgccccac cggtcgtcgg tgagccaggt ccacagacgc acgcggcggg
      841 tttccgggcc ctcgttcacg atgcgggtcc gcaccgagac cgtggccgcg gccgagtcca
      901 ccgacggcgt cgtgaccgtc gtgccccaat gggcgatgtg aagcggcggc accagcgata
      961 gccatgcgtg gcggtagatg ccgctccccg tgtaccagcg agagttgggc tgcttcgaat
     1021 tgtccacccg cacggcgacc acgttcgcgc ccgtgcgcac ttggtccgtc acgtcatgca
     1081 tgaacccgat gtagccgtag ggccggtgtc cgacgtgcgc gccgttcacc cagacgtcgg
     1141 agttctggtg cacgccgtcg aactcgaccc agaccctgcg atcgaccggg tccacggtga
     1201 acgtgcgacg gtaccacccg acgcccgtcg gcaggtaccc gccccttccg gtggtcgccg
     1261 cctgctcgtc gtacggcccc tcgatggacc agtcgtgcgg aagatccacc atgcgccacg
     1321 cggagtcgtc aaatccgggc gcctcggcgc ccgcgggatc gcccgtcgta aaccgccagc
     1381 cgaagtccat gaggaggcgg tcgcgcgtgg cggaatcctg ccgggccagg gctgggcccg
     1441 ccacgaggag aaggagcgcc agggcggcgc tgaggcgttt catgaggttg ccggatcgcg
     1501 tggtcgacga gcggccccgg ttactggatg agaaggatcc gctcgatctc gaagccgcgc
     1561 gccgcggagc gctcctccgc cggtacggcg tcgccgccga tgacgatctg gagcccgtcg
     1621 aggttcgcga ggtcgatcgc cgcagatccc gtcgcggctt ccagcgtgta gggcaggaag
     1681 ccggggtagg gccgcggaag cagtgcgagc ggcacccggc gcagactcgt gagcgggacc
     1741 tcgacgtcct gccacgagcc tgtcagaggc acgctgacgg cccaggcggt gccgttccgc
     1801 tcgatgaggg ccacctggat cgactcggtc tcgctctcgg tggccctccc gagcacgcgc
     1861 agcacggcat ccgatccgag cgtggtcgcc ccccggaggt gctctgccgg cagccaggtg
     1921 cggaccgcca ggtggtgcgg ctcgggctcc aggttcgtcg ccacggcctg cagcgcgagc
     1981 gagcccggga gcgtgccccc caccaacgaa gtgcggaagc ggacgtagcc ccacggattg
     2041 ggatagagca tgcggtccat gtcgcgccgc gcgtcgaaca ggaccaccgg gacgcccgcg
     2101 ccgacgaccg ggacccggta gaccgcgtcc gagacgaagt cccagtccga cggctgcccc
     2161 tcgacggccc ccgggaaggt ggtcgccgaa ccgccgcggt acacgacgat ccggtaatcg
     2221 aggagccctc cgcgcacgcg ttcggcgggc acggtggcct tgaaatcgta gccggaagcc
     2281 cgctccagcc ggggcgggcg cacgcggccg aagcgtccga atccgccctg gcctgctacg
     2341 aggaccaggg ccgagtcgac cgggctatcc gaaacgatgc gggcgcggac ggtgaagggc
     2401 gatccctcgg cgagctcgac cggcggctcg tgcacgagct ccggctcccc ggcgttcgcc
     2461 ccgggcgccg cgaacgggcg ccggtccgcg ggcgcgtccg gcacctcgat cccgctccgc
     2521 gtcaccaggt acacgcccgg gcggatgtcg aaggtcgtgc cgtcgaccgc ggggcggtga
     2581 tcgttgcctt cgttcgccgg cgtcaccgtg aaccccggtc ccaggtcggg aaggtcgatc
     2641 cgcatcggcc acgtccccca ccggacgcgc gccagggcgc gatcgaggct cggtcgctcg
     2701 aagggatcat cgatccagac gacgtccggg tacacctcca gccgccacac gcccccggac
     2761 tcgcggtcca ggaagtacgc ccccgtgccc tcgtaggcga cgaccgggga gcttccgaca
     2821 ccggccaagc ggagcagacg gtccggcgcg ggcggagcgg tgcccgtcga gttcgaatgc
     2881 aggaagacgg tctccgccgc catctcgctc aggtcctcct cgtagctcac gcggaacggg
     2941 ccgaacgtgg ccgattccgg gtacgccccg aacacggagc cccgcggcac ctgccggaac
     3001 acctcgcccg cgatcatgaa gctgatggcc ttgccgggcg catagaccag gttcaggaag
     3061 tgcgtctggt actcggtgtt gctccaggcg atcgccagcg ggtcgtaggc gaactgggcg
     3121 gcgaactgga agcccgcacc ccggaaggcg cgcgccatcg ccgggtacat gtacgagccc
     3181 gccacgtcgg ccgcgtcgaa ctcgtagacc atgagcgcct tgtccgcgca gtcgggcgtc
     3241 tccgtgaacg gcgtcggata ccggtccacg ttcggcagca tgtttccccg gatggtcgag
     3301 ccccgcacga ggcccgtggg gtaccactgg cacgaaacgc cctggatccg ggccgcgcag
     3361 acggcctcgc cgtgccgctc gccgtatccc tggctgatgt tgtagaagat cggcttcgcg
     3421 aatcccgcac cgcggagcgc atcggcgagc gcgtcgatga accgcgtggt ctcttccggc
     3481 ccacccggat ggctcggctc gttgaagatc tcgacggcga tgatgtccgg atcgtcccgg
     3541 taggacttgc ccgtgtacgg gttgacgtgc gcgatgaact gctccaggta gttccgctgc
     3601 gccacgcgtg cgacggagtc gaccgccatc tcgcccttgc cgtagaagtt cgagaagccc
     3661 gtcgactcct ggtcccgctc ggggtagccc gtgccccacc aggcgatggg cgtcaggatc
     3721 gacttgacgc cgtgctcctc gagccgcgcg agcaggtaat ccagcaggtc caggtggtcg
     3781 ttctcgacca ggttgccgtc gcggtcgctg atctcccggt cccagacgtg gatgcggaac
     3841 gcgtcgagcc cgagccgtgc caggtggttc acgtccgcgt cgatcgcctg cttcctgtcc
     3901 gcgcccacgt acccgagggc ccggtaggcg aaggcgtgcg gaagcgtgta gttcacgccg
     3961 aagagcgtca cctcctcgcc ggaggcagtc cagcgcatga cgccccggtc gtcgaccgag
     4021 acgggtgggc cgggctgggc atggatcggc agggcggacg ccccggccac gagcacgagg
     4081 ccgagcatcc gcgggaatcg gacggacatg ttcccggagc gtttgagaag cggggaaagg
     4141 gacgagggga gcggccttcc ggatcagtgg agcagcagca tcgcgcgcgc ccgggaggtc
     4201 gtcgagagcc cgttctgcat cgtaaggcgg tacaggtaga tgcccgtcgt cgccggagtg
     4261 ccgtcctcga gcagcccgtc ccaggcggcc gcgtgggtcc ccggagcctg gacggcctcg
     4321 tcgagcgtgc ggaccgtgcg gccgagcgtg tcgtggatgc tcagccggac ggaccccgcc
     4381 gcctgcagtt cgtacgcgat cgtcgttccg ggattgaagg ggttcgggta gttctgcgcc
     4441 agggtgaagc cggcgggaac ggtcccgtcc tccacggccg tgccgcccga taccgtgagg
     4501 cggacgttgt cgacctcggc ccatgcgtcg acttcggtcg tcgtgttctt gacggcgacg
     4561 ccgagcctgt tgccgatggc cacgaggccc atgtcgtcgg ccgccgccgc cagggtcagg
     4621 gtaaccatgg gctcgctgat gccctcgaaa tcatgctgcg cggtcccgac gatcgtgcgc
     4681 gttcccgcga catcgtagta cagacgcact tcgaagagat tggtctgcca gctgctccgg
     4741 gcatccgcct cgagcgtata cgtctcgccg gcggcgatca cgtggtcggt cagctgccag
     4801 atctcggggt cgttgttgaa cagccacgcc gcgcagacgc cgtccgtcgc gttggcgttg
     4861 tccgacacgc cggaatcctc gggatccgtg tcggtgctcc atccgggcac gcccgtgttg
     4921 aagttggtcg agcgggtgca gggcgtggtc ccgcccgccg tctcgaagct cgggttcacc
     4981 agggtgatcg tctcctgggc cagcagcgcc gtcgggagcg cgagcgctac gccgacgacg
     5041 agcagcagca tcgatcgtac cggattcctc atgatggcac ctcctgtcga gtggattgcg
     5101 gagcgcggta cggttcctgg cggaccgccc cccgggtgta cgtgtcaaag accgtgatca
     5161 cgggcagcgc gatgccctcc gcatcggggg gcctgcgccg ccgtgcagac gggaatcagc
     5221 gcggcggctg gaaggaccgt cagaggtcgg ctccggacca tgtcgcctca gtagcccgga
     5281 ttctgctcga tgatgccccc gccttcctgg atgacggact gcgggatcgg gaacagcacg
     5341 tggtgcggct gggcgttggt gatgcccgcg tcgcgcgcga cctggatgaa cctgccgtgc
     5401 cggatcaggt ccatgcggcg caggccttcc ccgaagaact cccgggcccg ctcgtccagc
     5461 agcgcgtccc ggaactggtc ctggttcatg ccgctgaacc cttcgagctc ggcccgctcg
     5521 cgcaccaggt tcgcgtactc gtacgcctcc gcgggcccaa cctgctcgtt gatggcctcg
     5581 gccagcgaca gcaggacctc ggcgtagcgg aataccggca cgtcgaacgt cacgccgttc
     5641 gagctcgtgc cggagatgtt ggtgaacttc aacggcacgg cgccgcgcat gccgctgtta
     5701 cggtcccggg tctgcccggt gcgcgtgaca tagctcgaca ggagggcgtc ctgccgctcg
     5761 tcatccgcgt cgtacttgtc gtagtagtcc cagggcatcc agaagccgta ccagccgggt
     5821 tcgcgcgtga tgtcgccgct cgatccgaag ttggacggga tgatctcctg gatgtaccag
     5881 ttgggcgcgg actcgttgac cgggaccgcg tagatgatct ccatgttctg ttcgttgttg
     5941 aacacgtccg ggtagtaggg ctccaggccg taccccatgc ccatgatgtc gcgcgcgacc
     6001 gcctcggcct tcgcccactc gtgctcgtgc atgtacaggc ggaggagcac catgcgcgcg
     6061 gcccccttgc tcacgcggcc ccagttggcg acgtcgccgt tgtacatgtc gggaagggcg
     6121 ggtatcgcct cgagaaggtc ggtctccatg gcctccacgt agtcggtctt cgtcatccgc
     6181 ggcgtgatct ccgtgtcgta cagggtctcg ggatcgagct tcgcgttcac cggtccgaag
     6241 aagtcgtaga tgacgaacat cagccaggcc cgaagggcct tggcctccgc cacgtacttc
     6301 gccttcacgg cgtcgggaac gtcggcctcg gacttctgga tcttgtcgat cacgtcggtc
     6361 gcgcgcgcga cgtagcggat cttcgcgtac gtcggcccgt tgccgccgtt gaacgtgttc
     6421 ggcccccaat cgaagttgtc gaacg
//
LOCUS       DBNHQZ010000412         4128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_527904, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000412 DBNHQZ010000000
VERSION     DBNHQZ010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_527904"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16YU_11220"
     CDS             <1..615
                     /locus_tag="AC16YU_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6065840.1"
                     /translation="SLAVVLAVTASALVFGSLTGYALAKLKWRGRDAVFSVILFTMMV
                     PFMVMLIPLYTMVVTFGWTDSLTGLVVPFMMNATAVLILRQSFVTVPTDVLDAARVDG
                     CSEPRILFTIVWPLSIPALVTAGIIVFIGSWNEVLWPIMVIRSEDWMTMPQMVALFSV
                     GGGAQARLGPQLAAAVLLALPIILVYIAFQRHFVASLASTGVKG"
     gene            635..2050
                     /locus_tag="AC16YU_11225"
     CDS             635..2050
                     /locus_tag="AC16YU_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065841.1"
                     /translation="MVSVWSFAILGCLALPSGCDSADDNHGGQPADAWFNSAHLDHLG
                     EEIARTGGSYRIIHIYSEAPDYGWVGDADEGVAALDDAARAAVVYLHHYEYTGDEASA
                     EKARQLLRFIMYMQRGNGLFYNFVWNADLDINETHPNSRADAFEWWAARAVWALGTGI
                     RVLGEEDSTMVGAMVERVRLTYPHLRSLLAHYGEYTNRGGRSVPLWLIGETGADATSE
                     LLMGLVAIQSARPDAEVSDMIARFAEGIAHMQYGSMNSFPYGAHASWIDQWHGWGNSQ
                     TQALAEAGYTASAEFEAEHFYPRLLVEGWMHSFSLDDPGSVREFEQIAYAVRAVSVGL
                     IRLFEATGDARYAKMAGLAASWLTGNNVAGETMYDHASGRGFDGIGQNSVNRNAGAES
                     TIEALFAVLEVDRYPQARQWLEARAGDAVHLTRDGKEYAYRVFTSGSGDSAIRIGVVM
                     NLTDERLSLLENSALDQFLAG"
     gene            2070..3080
                     /locus_tag="AC16YU_11230"
     CDS             2070..3080
                     /locus_tag="AC16YU_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR6065842.1"
                     /translation="MKASLFTRTPDPILVPSADLPWAAGAVFNPGAWRDGDVVHLLFR
                     AIPGGHRRILLDSPGTGETVFGFDPYVSAIGYASSSDGVRFECRPVPFISPDTPIDHF
                     GAEDARVSRIDDTYFITYTALSEPAFGPRRAWHIGLASTEDFIGLRKHGAIGPPVASK
                     DSVIFPRRIRGRIVMLHRIIPDIQLISFDDLDQMMSPPESAWRRHLDTLPEHVVMRPR
                     AGWESEKIGAGPTPVETAEGWLLIYHGVDEHHVYRVGMALLDLDDPQRVISRTRTPVM
                     EPEAEYECVGDVNNVVFPQGAVVIDGTLHLYYGAADRVIGHATAPLSALVDYLRSGAG
                     NP"
     gene            3127..>4128
                     /locus_tag="AC16YU_11235"
     CDS             3127..>4128
                     /locus_tag="AC16YU_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR6065843.1"
                     /translation="MTPERLHGGRPVLTAEPAHAWERRVVLNPAAVLVEAGPGLEALF
                     NAWDLGPDGRQRLREAGGACVMLYRAQGDVEPEKACSPSYLGLAVFTPLLDCVMRRPG
                     PVIEPIERFHGLGVEDPRCTRVGDTYYLYYTGYSSRRMDGKGPDGNVQICLATTRDFV
                     EWNLLGPVPGDLNRVPNKNAALLPEIVDDKWLLLHRPMDGPDAMTIHLAEADHPEGPW
                     RSRGALMQSHPYAEFVRSWLGAGGPPMPLGEDRFLMVYHQGHYTPEMRREYDIAVALL
                     DFGLPDPVVRRIEPLMRPSLALERLGDPDLGVDNVLFTCANFRWGDLLVVPYAGADSR
                     "
BASE COUNT          716 a         1278 c         1367 g          767 t
ORIGIN      
        1 agcctggccg ttgtactcgc cgtaacggct tcggccctcg ttttcggatc cctgaccggc
       61 tacgccctgg ccaaactgaa atggcgtggg cgggatgcgg tattctccgt catcctgttc
      121 accatgatgg tgccgttcat ggtgatgctg ataccgctgt acacgatggt ggtgacgttc
      181 ggatggacgg attccctgac cgggctcgtg gtgcctttca tgatgaacgc cacggcggtc
      241 ctgattctgc ggcagagttt cgtgaccgtg ccgacggacg ttctggacgc ggcccgagtc
      301 gatggatgca gtgagccgcg catactgttc accatcgtct ggcccctctc gattccggcc
      361 ctggtcactg ccgggatcat cgtattcatc gggagctgga acgaggtgct ctggccgatc
      421 atggtgatcc gaagcgagga ttggatgacc atgccgcaga tggtggccct gttctcggtc
      481 gggggtggag cccaggccag gctggggcca cagctggctg cggccgtgct gctcgcactg
      541 ccgattatcc tggtctacat cgcctttcag cggcatttcg tcgcgagtct ggcatccacg
      601 ggggtgaagg ggtgacatga aagcaacgat gacgatggtc tctgtctggt cgttcgccat
      661 actgggttgc cttgcgctcc cgagcgggtg tgactcggcg gacgacaacc acggagggca
      721 gcctgcggat gcgtggttca actccgcgca tctggatcac ctgggcgagg agatcgccag
      781 gaccggcggc tcgtaccgca tcatccacat ctactccgag gcgcccgatt acggctgggt
      841 gggggacgct gatgaaggag ttgccgcact ggacgacgcc gcgagggccg cggtagtcta
      901 cctgcaccat tacgagtaca ccggtgacga ggcatcggcg gaaaaggcgc ggcaactgct
      961 tcggttcatc atgtacatgc agcggggaaa cggcctcttc tacaatttcg tctggaatgc
     1021 cgatctcgac atcaacgaga cgcatccgaa cagcagggcg gatgccttcg aatggtgggc
     1081 agcccgggcg gtctgggcgc tgggcacggg aatccgggtg cttggagaag aggactcgac
     1141 catggtcggg gcgatggtcg aacgcgtacg tctcacgtat ccgcacctgc gctccctcct
     1201 ggctcactac ggtgagtaca cgaatcgggg cggcaggagc gttccgttgt ggcttatcgg
     1261 ggaaaccggg gcggacgcca cgagcgagct gttgatgggt ctcgtcgcta tccagagcgc
     1321 gcgcccggat gccgaggtat cggacatgat cgcgaggttc gcggagggca ttgcgcacat
     1381 gcagtacggc tccatgaact cgttcccgta cggcgcgcac gcttcgtgga tagaccagtg
     1441 gcacggctgg ggcaactcac agacccaggc actggcggag gccggttaca cggccagcgc
     1501 cgagttcgaa gccgagcatt tctaccccag gctcctggtg gagggttgga tgcattcgtt
     1561 ttccctcgac gacccaggca gcgtccggga attcgaacag atcgcctatg cggtgcgggc
     1621 cgtgagcgtg ggcctcatcc ggttgttcga ggcaaccggc gacgcgcgct acgcgaagat
     1681 ggcggggctc gctgccagct ggctgaccgg caacaacgtc gccggcgaga ccatgtacga
     1741 tcacgcctcg ggtcgcggat tcgacggtat cggccagaac tcggtcaaca ggaacgccgg
     1801 tgcggagtcc acgatcgagg ccttgttcgc cgtcctggaa gtcgatcggt atccccaggc
     1861 gcgccagtgg ctggaggcaa gggccggaga tgccgtccac ctgacgagag acggcaagga
     1921 gtacgcctac cgtgtgttca cgagcggaag tggagactcc gcgatccgta tcggcgtcgt
     1981 catgaacctg acggacgaac gcctgtctct tctcgaaaac agcgcgctcg accagttcct
     2041 ggcgggctga cccaacatag tcggaccgaa tgaaggcatc cctgtttacg cgaactcccg
     2101 atcccatcct cgtaccgagc gcggacctgc cctgggccgc aggcgccgtg ttcaacccgg
     2161 gagcatggag agacggtgat gtcgttcacc tgcttttccg ggcgatcccg ggaggacacc
     2221 ggcggatcct gctggacagc ccgggcacgg gcgagacggt cttcggattc gatccctacg
     2281 tctcggccat cggctacgct tcgagctccg acggggtgcg attcgaatgc cggcccgtac
     2341 ccttcatctc acccgacacg ccgattgacc atttcggcgc ggaggatgcc agggtgtcga
     2401 gaatcgacga cacctacttc atcacctata ccgcgctgag cgagccggcg ttcggtccga
     2461 gacgcgcgtg gcacatcggg ctggcctcca cggaagactt cattggcctg agaaaacacg
     2521 gcgcgatcgg acctccggtc gcgtcgaaag actcggtcat cttcccgcgc cggatccggg
     2581 gtcgcatcgt catgctccac aggatcatcc cggacattca gctgatctcg ttcgatgacc
     2641 tggatcagat gatgtcaccg ccggaatcgg cctggcggag gcatctggat acccttccgg
     2701 agcatgtcgt catgcgcccg cgtgccgggt gggagtcgga gaagatcggt gcaggtccga
     2761 caccggtcga gaccgcggag gggtggctgc tgatctacca tggggttgat gagcaccacg
     2821 tgtaccgggt cggaatggca ctcctcgatc tggacgatcc gcagcgcgtg atcagccgca
     2881 cgaggacacc ggtaatggag cccgaagcgg aatacgagtg cgtaggtgac gtgaacaacg
     2941 tcgtcttccc gcagggtgcc gtcgtcatcg acggaacgct gcatctgtac tacggtgcgg
     3001 ccgaccgggt gatcggtcac gccaccgctc ccctttcagc cctggtcgat tacctgcgta
     3061 gcggtgccgg caatccgtga cccatcattc cccggccgcg tgcggacgat caccgagcca
     3121 ccccccgtga cgccggaacg ccttcacggg ggtcggccgg ttctgacagc ggaacccgcg
     3181 cacgcgtggg agcgtcgggt tgtgctcaat cccgcggccg tactcgtcga agccggccct
     3241 gggctcgagg cgctcttcaa cgcctgggac ctggggccgg acgggcggca gcggcttcga
     3301 gaggccggcg gagcgtgcgt gatgctctac cgtgcccagg gggacgtcga acccgagaag
     3361 gcatgctcgc cctcctacct gggactggcc gtgttcaccc cgctcctgga ttgcgtgatg
     3421 cgccgacctg gaccggtgat cgaaccgatc gagcgctttc acggcctcgg cgtcgaagac
     3481 ccgcgatgca ccagggtagg agacacctac tacctgtact acacgggcta ctcgtctcgg
     3541 cgaatggatg ggaaggggcc cgacggaaac gtacagatct gcctggccac gacgcgtgat
     3601 ttcgtcgagt ggaatctgct cggtccggtc ccgggtgatc tgaatcgcgt tccgaacaag
     3661 aatgccgcgc tgctccccga gatcgtcgac gacaagtggc tgctcctcca tcggcccatg
     3721 gatggacccg atgcgatgac cattcacctc gcggaagcgg atcaccccga gggtccctgg
     3781 cgctcgcggg gggccctgat gcagagtcac ccgtacgccg agttcgtccg gtcgtggctc
     3841 ggtgccggcg gtccgcccat gccactcggc gaggacaggt ttctcatggt ttaccaccag
     3901 ggccactaca cgcctgaaat gaggcgcgag tacgacatag cagtggctct ccttgatttc
     3961 gggttgcccg atcccgtcgt gcgccggatt gagcccctga tgcgtccctc cctcgctctc
     4021 gaacggctgg gagacccgga tctgggggtc gacaacgtac tgttcacctg cgccaacttc
     4081 cggtggggag acctgctggt ggtgccgtac gcgggtgcgg acagtcga
//
LOCUS       DBNHQZ010000413         5204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3100944, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000413 DBNHQZ010000000
VERSION     DBNHQZ010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3100944"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1513)
                     /locus_tag="AC16YU_11240"
     CDS             complement(101..1513)
                     /locus_tag="AC16YU_11240"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR6065844.1"
                     /translation="MSELSKIEREILAAAREPVRADAASAYHGALRSVDRELMLESAS
                     RFGTPQYFLDVDALRRRAGLFATTMSAHVPRAECCYAFKCNDLPALVGALRGEGFHAD
                     VSGQFELELALRMGFDRILFSGPGKTERELGLAVEHAERVIVNLDNLDEIDLLGSLTP
                     ANGERPRLAVGFRVNTATARGGAWWKFGFELDELGEAIRRVDACPGLEWAGIQFHGSW
                     NKTPAGYVAQIERIAGWLAENVAAERLRTLRFFDVGGGFYPEDQGLLLQSEDRGTLLD
                     LAGRRIGDRAGAFREAGIDPVGFQVTPVEPLDAFARAIGEAVRRHVLPLSPDVTVYFE
                     PGRYIATFATTILVRVMAVKSHGVIVDGGINMLGDYKLAEYSFAPVVNLSRPSDDLRR
                     RVICGSLCDPADLWGYSHYGDELRRGDVVAVLHQGAYTFSGAWRFIKSIPAYVAASGG
                     ELELARREETFDVRYAGCRM"
     gene            complement(1553..2848)
                     /locus_tag="AC16YU_11245"
     CDS             complement(1553..2848)
                     /locus_tag="AC16YU_11245"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6065845.1"
                     /translation="MDDNAYGDSLVAFPARDVGFAELHVFLRSLEAYGVLDRVLLACP
                     AHYFEVHPEPVPLHVDEEFRDPDRAVDRIVARAADAGRRIGGVIGVDEDLQFGLSSAL
                     AARSGVPFHSDDACVRASNKFVQKTWFRRAAVPTSPFALVGDPSDADEVGFPCVVKAV
                     TSYASQYMGRCDDRQQLAATLERLRGAAGRAGDDPRFRPQTVSTGGVTLRLDPRRDFL
                     VEAFVRGEEYSCDVLVRGGEVHVVRVARKVQGPRFGFFDGAVLLGRREIDRCGVGLDR
                     LTEVCRRIASALAVREGVCMVDFRLPGGKIVVLEASIRPGLSATNHLVYETYGYTPLA
                     LLAMLLMGRAPEVRIPEEGAAAVYLNEGGRTWDARAVAALRERHGRDRVYVYDQSEDA
                     GPIPENDPSALIRGYVVVTGVTDLAWEAVVERALGGIGT"
     gene            3052..4716
                     /locus_tag="AC16YU_11250"
     CDS             3052..4716
                     /locus_tag="AC16YU_11250"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6065846.1"
                     /translation="MHPARIPAALLAVAFVASPVRAQFDPYDTGGPLLPEQASYDVSY
                     YDLNVRVNPADSTLRGHVRIVAGVHSPIDWFVIDLDPVLTIESISGAEPTVADLEFER
                     RGGRVWIQLPATRQPGSRVDVTVSYGGRPRIAPRPPWDGGLTWARTPSGAPWIATTCQ
                     QEGADVWWPLKEHVSDEPDSMAIRVTVPDPLVVASNGRFRGKSAAEPGWTTWDWFVST
                     PINTYNVALNIAPYVRLDTSFVSVAGGTVPVQFFVLPENERDGRNLLPEILDHLAFFE
                     DTLGPYPFRADKYGVAQTPHLGMEHQTIIAYGANFRNESMTRGTDWGFDALHHHELSH
                     EWWGNLVTNVDWSDMWLHEGFGSYMQPLYVERKQGPDAYTAYLRNSRRGLRNEQAVAP
                     RQVMSGKEVYNGDIYAKGAWVLHTMRYLLGDDAFFEVLRRQAYPDPALEAVTDDRQAR
                     FGTTDDFMKLAERISGSDLDWFFDVYIRQPDLPELVAERAGDTVTLSWSVPGDLPFPM
                     PVDVRIGDVTERVDLSAGGVELRIPEGMEFGIDPDDRVLRVGNRTR"
     gene            complement(4746..>5204)
                     /locus_tag="AC16YU_11255"
     CDS             complement(4746..>5204)
                     /locus_tag="AC16YU_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065847.1"
                     /translation="SDAGLESLFARRARSVDDPTSAGLAVPEGPVDAHWLLLPGPVAV
                     PEAHFADPAWQTVARFRSRHPALADGVHAKISDEPYAFYRGMRLDRGLEDVVLVVVGA
                     EGRLRLNVSRIFPDDVVLRDAVSGAIALVSFGQLSMTAGPGGLVLLELVD"
BASE COUNT          804 a         1914 c         1753 g          733 t
ORIGIN      
        1 tcgtgctgcg ggagttcctt aacgcggagc agaccgcctg agcagacgcg accgcggggc
       61 cacgcatcgc ccggatcgcg aacagggcgc caccccggcg tcacatccgg caccccgcat
      121 atcgtacgtc gaacgtctcc tcgcggcgcg cgagctcgag ctcgccgccc gaggcggcca
      181 cgtacgccgg gatcgacttg atgaagcgcc atgcgccgga gaacgtgtag gcgccctggt
      241 gcaggaccgc cacgacgtcg ccgcggcgca gctcgtcgcc gtagtgcgag tacccccaga
      301 ggtccgcggg atcgcacaac gagccgcaga tcacgcgccg ccgcagatcg tccgacggcc
      361 gcgagaggtt cacgaccggc gcgaaggagt actcggccag cttgtagtct ccgagcatgt
      421 tgatgccgcc gtccacgatg acgccgtggc tcttcacggc catgacccgt acgaggatgg
      481 tcgtcgcgaa cgtcgcgatg tagcgccccg gctcgaagta gaccgtgacg tccgggctga
      541 gaggcagcac gtgccgccgg acggcctccc cgatcgcgcg ggcgaacgcg tcgagcggct
      601 cgaccggggt cacctggaag ccgaccggat cgatccccgc ctcgcggaag gccccggccc
      661 ggtcgccgat cctcctcccc gcgaggtcca ggagcgtgcc gcgatcctcc gactgaagga
      721 ggagcccctg gtcctccgga tagaatcctc cgcccacgtc gaagaacctg agcgtgcgca
      781 gccgctccgc cgcgacgttc tccgcgagcc agcccgcgat cctctcgatc tgcgccacgt
      841 acccggccgg agtcttgttc cagctgccgt ggaactggat gcccgcccac tccaggcccg
      901 ggcaggcatc gacgcggcgg atggcctcgc cgagctcgtc gagctcgaac ccgaacttcc
      961 accaggcgcc gccgcgcgcc gtcgccgtgt tgacgcggaa gcccaccgcg aggcgcggcc
     1021 gctcgccgtt tgccggcgtg agcgagccga ggaggtcgat ctcgtccagg ttgtccaggt
     1081 tgacgatcac ccgttccgcg tgctccacgg ccaggcccag ctcgcgctcc gtcttgcccg
     1141 gccccgagaa caggatccgg tcgaagccca tgcgcagggc gagctcgagc tcgaactgcc
     1201 cggacacgtc ggcatggaag ccctctcccc gcagggcgcc gaccagcgcg gggaggtcgt
     1261 tgcacttgaa ggcgtagcag cactcggccc tcgggacgtg ggcgctcatc gtggtcgcga
     1321 acagcccggc gcggcggcgc agcgcgtcca cgtccaggaa gtactgcggc gtcccgaagc
     1381 gcgaggcgct ctcgagcatc agctcccggt ccaccgaccg cagggccccg tggtaggcgg
     1441 acgccgcgtc ggcccgcacg ggctcacgcg ccgcggccag gatctcgcgt tcgattttcg
     1501 aaagctcact catgccatat ccctgtgatg tccccgtgtg gatgttcatg gcctacgtcc
     1561 cgattccgcc cagagccctc tcgaccacgg cctcccacgc caggtccgtg acgccggtca
     1621 cgaccacgta accccggatc agcgccgacg gatcgttctc ggggatgggt ccggcgtcct
     1681 cggactggtc gtagacatag acgcggtccc ggccgtggcg ctcccggagc gccgcgaccg
     1741 cccgcgcgtc ccacgtccgc cctccctcgt tcaggtagac ggccgcggcg ccctcctccg
     1801 ggatgcgcac ctccggcgcc cggcccatca gaagcatcgc caggagggcg agcggcgtgt
     1861 agccgtacgt ctcgtagacg aggtggttcg tggcgctgag ccccggacgg atcgatgcct
     1921 cgagcaccac gattttgccg ccgggaagcc ggaagtccac catgcagacg ccctcccgca
     1981 cggcgagggc cgatgcgatc cggcggcata cctccgtgag gcggtcgagc cccaccccgc
     2041 accggtcgat ctcgcgccgg ccgaggagaa cggccccgtc gaagaatccg aacctcggcc
     2101 cctgcacttt ccgggccacg cgcacgacgt gcacctcgcc gccgcggacc aggacgtcgc
     2161 agctgtactc ctctccacgg acgaacgcct cgaccaggaa gtcgcgccgc gggtcgagcc
     2221 gcagcgttac gccgccggtc gacacagtct ggggacgaaa ccgggggtcg tcccccgccc
     2281 ggcccgccgc gccgcgcagc cgctcgaggg tcgcggctag ctgctgccgg tcgtcgcagc
     2341 ggcccatgta ctggctggcg tagctcgtga cggccttgac cacgcacggg aatccgacct
     2401 cgtccgcgtc ggatgggtcg ccgacgagcg cgaacgggga ggtcggcacc gccgcgcgcc
     2461 ggaaccaggt cttctgcacg aacttgttcg acgcgcgcac gcaggcatcg tccgagtgga
     2521 agggcacgcc cgaccgggcg gcgagcgcgc tcgaaaggcc gaactgcagg tcctcgtcca
     2581 cgccgatcac gccgcctatg cgccgcccgg cgtccgccgc ccgggcgacg atccggtcca
     2641 cggcgcggtc tgggtcacgg aactcctcgt cgacgtgcag gggaacgggc tccgggtgaa
     2701 cctcgaagta gtgcgcgggg caggcgagaa gaacccggtc cagaacgccg tacgcctcca
     2761 ggctacgcag gaacacgtga agctcggcga accccacgtc gcgggccgga aaggcgacca
     2821 ggctgtcccc gtaggcgttg tcgtccatgc ccgctccgtc cggtctcttg atgtgcccgc
     2881 ccggaccgcg cgcggggcgt tccctgtcgc gcggttccgg ccgcggcaga agatcggcac
     2941 gtgcgggcgg gcggccaata cgaatccggg acggccagcg gaatcgggct ttgattccga
     3001 cgcctgccgc tcgtagcgtt gggacccacc cggcaccgac cgacctccgc catgcaccct
     3061 gcccggattc ccgccgccct cctcgccgtc gccttcgtcg cctcgcccgt gcgggcccag
     3121 ttcgatccct acgataccgg agggccgctc ctgccggagc aggcgtcgta cgacgtctcg
     3181 tactacgacc tgaacgtccg cgtgaacccg gccgattcga cgctccgcgg gcacgtccgc
     3241 atcgtggccg gcgtgcactc gcccatcgac tggttcgtga tcgacctcga tcccgttctc
     3301 accatcgagt cgatatcggg cgcggagccg actgtggccg atctggagtt cgagcggcgg
     3361 ggcggccgcg tgtggatcca gctccccgcc acgcgccagc cgggttcccg ggtcgacgtc
     3421 acggtgtcgt acggcggacg cccacgcatc gcgccgcggc caccgtggga cggaggactc
     3481 acctgggcgc gcacgccgtc cggtgccccg tggatcgcca cgacctgcca gcaggagggc
     3541 gccgacgtct ggtggccgct caaggagcac gtctccgacg agccggactc gatggccatc
     3601 cgcgtgaccg ttcccgatcc gctggtcgtc gcgtcgaacg gccggttccg cggcaagtcg
     3661 gcggccgagc cgggctggac cacgtgggac tggttcgtgt cgacccccat caacacgtac
     3721 aacgtcgcgc tgaacatcgc gccctacgtc cgcctggaca cgtcgttcgt gagcgtggcg
     3781 ggtggcacgg tgcccgtgca gttcttcgtg ctgccggaga acgagcggga cggccgcaac
     3841 ctcctgcccg agatcctgga ccacctcgcg tttttcgagg ataccctcgg cccctacccg
     3901 ttccgcgcgg acaagtacgg cgtcgcgcag acgccgcacc tcgggatgga gcaccagacg
     3961 atcatcgcct acggggccaa cttccggaac gagtccatga cccgcggcac ggactggggg
     4021 ttcgacgcgc tgcaccacca cgagctctcg cacgagtggt ggggcaacct ggtcacgaac
     4081 gtcgactggt ccgacatgtg gctgcacgag ggcttcggct cgtacatgca gccgctgtac
     4141 gtggagcgga agcagggtcc ggatgcctac accgcgtacc tgcggaacag caggcgcggg
     4201 ctccggaacg aacaggccgt cgcgccgcgc caggtcatgt cgggcaagga ggtctacaac
     4261 ggggacatct acgcgaaggg cgcctgggtt ctgcacacga tgcggtacct gctgggtgac
     4321 gacgccttct tcgaggtcct gcgacgccag gcctatcccg atcccgccct cgaggccgtg
     4381 acggacgacc gccaggcccg cttcggaacg accgatgact tcatgaagct cgccgagcgc
     4441 atttccggga gcgatctcga ctggtttttc gacgtgtaca tccggcagcc cgacctgccg
     4501 gagctcgtgg cggagcgggc gggtgacacc gtcacgctgt catggagcgt gcccggggac
     4561 cttccgttcc ccatgcccgt cgacgtgcgg atcggcgacg tgaccgagcg cgtggacctg
     4621 tccgcgggtg gggtcgaact ccggatcccg gagggcatgg agttcggcat cgacccggac
     4681 gaccgcgtgc tccgcgtcgg aaaccgcacc cgctgacctc gcggcgagtc cctgccgccg
     4741 actcctcagt cgacgagttc gaggagcacg agcccgcccg gcccggccgt catcgacagc
     4801 tgtccgaacg acaccagggc gatggcgccg gagacggcat cacgcagcac gacgtcgtcc
     4861 gggaaaattc tcgagacatt cagccggagc cttccctcgg cccccaccac gaccaggacc
     4921 acgtcctcga ggccacgatc cagccgcatc ccgcggtaga aggcataggg ctcatcggag
     4981 atcttggcat gcacgccgtc cgccagcgcg ggatgccggg agcggaagcg cgcaacggtc
     5041 tgccaggccg ggtccgcgaa gtgcgcctcg ggcacggcca cggggccggg cagaagcagc
     5101 cagtgcgcat cgaccggccc ctcgggcacg gccaggcccg cggacgtcgg atcatcgacg
     5161 ctgcgggcgc ggcgtgcgaa caggctctcg agcccggcat ccga
//
LOCUS       DBNHQZ010000414         2650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3374572, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000414 DBNHQZ010000000
VERSION     DBNHQZ010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3374572"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1441
                     /locus_tag="AC16YU_11260"
     CDS             155..1441
                     /locus_tag="AC16YU_11260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6065848.1"
                     /translation="MMHEGHEKLQRPDVAAIPSAEPSLFDKCHRFFDSESLFAQVKNA
                     GLYPYFRPIERNDGSHAIINGREVIMAGSNNYLGLTSDPRVQEAAAQAIRQYGTGCTG
                     SRFLNGTLDIHIQLEERLARFMGREACVLFSTGYMTNQGVIQALASKGDIIFSDKDNH
                     SCIVAGTQVSMAETWRYRHNDMVHLRRLLDRASVEYPEAGKLIVTDGVFSMSGTFARI
                     PELLELAEEFGAALMLDDAHAVGVVGPGGHGSASHFGLQDRVHLTTGTFSKSFASLGG
                     FCVGDHDVIEFIRHQASAHIFSASMPPANVGTVLKCLEILEAEPERVERLWKISDYMR
                     AGFRDAGFNVWSSQSPIIPVVVGDLHTCFRFWADLLEEGVFVNPVVPPAVPKGQSLMR
                     TSYMATHTDEDLDFILNAFVRVGRRHGVIGDNGVAG"
     gene            1520..>2650
                     /locus_tag="AC16YU_11265"
     CDS             1520..>2650
                     /locus_tag="AC16YU_11265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6065849.1"
                     /translation="MARVQVIPVDGLGGRKSFIDFPYAHYRNDPNFVPPLRLDAARLL
                     NPRKNAFFEHGRIQPFLARADDGRVLGRIAAIVNGEHLKKHGDGVGFFGFFESVDDRE
                     VSTALFDAAGDWLRQQGLRSMRGPANPSLNDVAGLLVDGFLTPPYILMAYNPPYYERL
                     VLEYGFERVMTMWAYFVHRKYADLDRFKRGADLVFRRYPSLHIRNLDMARFDEEVRTV
                     RSIYNDAWSHNWGFVPMTEAEFAQLARNLKQIVDPRMAFLAEIDGEVIGFGIAIPNLN
                     QVLQRIRNGRLLPTGLFKLIMADFLGGIQEVRLPLLGVRRAYHGRGIDAALMLQFIEK
                     VPPIGFPSCEMSWILESNPAMKNALEALGGVRDKEYALYERAL"
BASE COUNT          440 a          885 c          849 g          476 t
ORIGIN      
        1 cggccattta aggtggcctt gcccgtcgtt aatgtcgtat tattgacgtg gtcacgcgcc
       61 attgcgggca gcctccgcgg gagtgagccc gactggtcgt tgccaacgga ggcgggacca
      121 tccgcccttc catcccgctc cccgatcggt ccacatgatg cacgagggcc acgaaaagct
      181 ccagcggccg gatgtcgccg ccattccgtc cgccgagccg tcgctgttcg acaagtgcca
      241 ccgcttcttc gattcggaga gcctcttcgc ccaggtgaag aacgcggggc tgtatccgta
      301 tttccgcccg atcgagcgca atgacggttc ccacgcgatc atcaacggtc gcgaagtgat
      361 catggcgggg tcgaacaact atctgggcct gacgtccgat ccccgggtgc aggaggccgc
      421 cgcgcaggcg atccgccagt acggcacggg ctgcacggga agccgcttcc tgaacggcac
      481 gctcgacatc cacatccagc tcgaggagcg gctggcgcgg ttcatgggcc gggaggcctg
      541 cgtcctgttc tcgaccgggt acatgacgaa ccagggcgtc atccaggccc ttgcctcgaa
      601 gggcgacatc atcttttctg acaaggacaa tcattcgtgc atcgtcgccg gcacgcaggt
      661 cagcatggcc gagacgtggc gctaccggca caacgacatg gttcacctga ggcggctcct
      721 ggaccgcgcc tcggtggagt atcccgaggc cgggaagctc atcgtcacgg acggcgtctt
      781 ctcgatgtcc gggaccttcg cccggatacc cgagctcctg gagctggccg aggagttcgg
      841 cgcggcgctc atgctcgacg acgcgcacgc cgtgggcgtc gtgggaccgg gcgggcatgg
      901 cagcgcatcc catttcgggc tgcaggaccg cgtccacctg acgacgggca cgttctcaaa
      961 atcgttcgcc tcgctcggcg gattctgtgt cggagaccac gacgtaatcg agtttatccg
     1021 ccaccaggcg tccgcccaca tcttcagcgc ctcgatgccg cccgccaacg tgggcaccgt
     1081 gctcaagtgc ctggagatcc tggaggccga gcccgagcgt gtcgagcggc tctggaagat
     1141 ctcggattac atgcgcgccg gattccggga cgccggtttc aacgtgtggt cgagccagtc
     1201 ccccatcatc ccggtcgtcg tgggcgacct gcacacgtgc ttccgcttct gggcggacct
     1261 gctcgaggag ggcgtcttcg tgaatccggt ggtcccgccc gctgtcccga agggccaatc
     1321 cctgatgcgc acgtcctaca tggcgactca caccgacgaa gacctggact tcatcctgaa
     1381 cgcgttcgtg cgggtcggac gccgtcacgg cgtcatcggt gacaatgggg tcgcgggctg
     1441 accgtgattc gccgcagtgc cggaaccgtg acgtcctccc ggtccctccg ccttgtcgtt
     1501 tcgagccacg agcgtgaaca tggccagagt gcaggtcatc ccggttgacg gtctcggagg
     1561 ccggaagtcc ttcatcgact tcccgtatgc gcactaccgg aacgatccga acttcgtccc
     1621 gcccctgcgc ctggatgccg ccaggctcct caatccgcgg aagaacgcgt tcttcgagca
     1681 cggacggatc cagccgttcc tcgcccgggc ggacgacggg cgcgtgctcg gccggatcgc
     1741 ggcgatcgtg aatggcgagc acctgaagaa gcatggcgac ggggtcggct tcttcggctt
     1801 tttcgaatcg gtggacgatc gcgaggtgtc gacggccctc ttcgacgccg cgggcgattg
     1861 gctccggcag cagggactgc ggtccatgag ggggccggcg aatccctccc tgaacgacgt
     1921 tgcagggctg ctcgtcgatg gcttcctgac gcccccgtac atcctgatgg cctacaatcc
     1981 gccgtactac gagcggctgg tgctcgagta cggcttcgag cgcgtgatga ccatgtgggc
     2041 ctacttcgtg caccggaagt acgcagacct ggaccggttc aagcgcggcg ccgacctcgt
     2101 tttccgtcgc tacccgagcc tccacatccg gaacctggac atggcccggt tcgacgagga
     2161 ggtgcgcacg gtccgctcga tctacaacga cgcctggtcc cacaactggg gcttcgtgcc
     2221 catgaccgag gccgaattcg cgcaactggc ccggaacctg aagcagatcg tcgacccacg
     2281 gatggccttc ctggccgaga tcgatggcga ggtgatcggg ttcggcatcg cgatcccgaa
     2341 cctgaaccag gtcctccagc gaatccggaa cgggcgcctc ctgcccaccg ggctcttcaa
     2401 gctgatcatg gccgactttc tcggcggcat ccaggaagtg cggctgccgc tcctgggcgt
     2461 ccggcgggcc tatcacggcc gaggcatcga tgcggccctc atgctccagt tcatcgaaaa
     2521 ggtgccgccg atcggcttcc cgtcctgcga gatgagctgg atcctcgaga gcaacccggc
     2581 aatgaagaac gcgctggagg cgctcggcgg ggtccgcgac aaggagtacg ccctgtacga
     2641 gagggcgctc
//
LOCUS       DBNHQZ010000415         3817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3238429, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000415 DBNHQZ010000000
VERSION     DBNHQZ010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3238429"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="AC16YU_11270"
     CDS             complement(<1..528)
                     /locus_tag="AC16YU_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065850.1"
                     /translation="MAIFEEPVPARRMRFPTPCLAILPLLAAAGCAEPPLAYVGGGAC
                     TGCHPAETVAWTGSDHDLAMQPADSSTILARFDGSSLAHAGLETTFFRDGDRFLVRTD
                     GPDGSMQDFEIRYTFGVRPLQQYLVELPGGRLQALPFAWDARPGEEGWYHLYPDDSLT
                     IRDPLHWTGVFQNWNH"
     gene            596..1018
                     /locus_tag="AC16YU_11275"
     CDS             596..1018
                     /locus_tag="AC16YU_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR6065851.1"
                     /translation="MAELKTRPSDVSVESFLDSVADDGKRADSRALLDLMREVTGEEP
                     VMWGGSIVGFGAYRYRYDSGREGDWFLTGFSPRKQNLTLYVMAGFDRYDELMARLGKH
                     KTGKSCLYLNRLSDVDAGVLRELVGRSVAHMKSMYPAS"
     gene            1020..1244
                     /locus_tag="AC16YU_11280"
     CDS             1020..1244
                     /locus_tag="AC16YU_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6065852.1"
                     /translation="MPWYDYRCRRCGSVFEIRRSMNERSDGVSVECSECGSAETERVF
                     RPIAVGVAGSPRPEAPQGASCGPHCACHPN"
     gene            complement(1269..2651)
                     /locus_tag="AC16YU_11285"
     CDS             complement(1269..2651)
                     /locus_tag="AC16YU_11285"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR6065853.1"
                     /translation="MRRALLLLAISAAACSPEPPEALGPGRRNTLGDEAARLFEANLM
                     AAWYPRAIDTVAGGYLSDFDADWNAAGPQHKMIVTQARHVWTTARAGEFLGDPETWVP
                     RSAHGVDFLARSMWDAEHGGFFWMVTREGAPVPEPDGRLLKQAYGNAFAIYGLAAYAD
                     VSGDAAALSLARRAFGWLDAHAHDPVHGGYFNYLELDGTPLRAGLADRPADRGYDPTP
                     PKDQNSSIHLLEAFTELYRVWPDSVLRVRLAEMLHLVRDTIVTAPGTLQLFFLEDWTP
                     VSYRDSSEAKRRANEYLDHVSFGHDVETAYLVLEAAEALGDVGEVVRSLDVGKRLVDH
                     ALRHGWDRENGGFIDAGYYLAGDSTLTVLRDTKNWWAQAEGLNTLLLLGDLYPEDTLT
                     YHARFLEQWAYVSAFLVDHERGGWYEGGLDRQPELARGPKAHIWKSAYHDGRALMNVA
                     RRLAGAYGPR"
     gene            complement(2658..3761)
                     /locus_tag="AC16YU_11290"
     CDS             complement(2658..3761)
                     /locus_tag="AC16YU_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR6065854.1"
                     /translation="MDRRTFLRTGSAAAVGALALPACRTSAPEAPAVTQPVIPRWRGF
                     NLTELTGGREVRPFLESDFEWMAEWGFDFARLPMSYWSWSGPDDWMRIDDSVLAHVDQ
                     AVDFGRQYGIHVNLNFHRIPGWCVNGPELEPHQLFTGPPESQAMALEAATHHWRHFAE
                     RYRGVPSDRLSFDLFNEPPWMDDHARYIEIARALVGAIREVDPDRLIVADGADIGQTP
                     VMGLVDLRLVQSTRGYLPKMVSHFTATWVPENEFESFEVPSWPMVDMNGVLWNRETLR
                     ERLIVPWLPLARAGVPVHVGEWGCFIRTPHDVALAWMADLLSLWKEVGWGWAMWNLRG
                     SFGIVDSGREDVAYEEWRGHRLDRGMLELLREG"
BASE COUNT          590 a         1313 c         1354 g          560 t
ORIGIN      
        1 gtggttccag ttctggaaga cgccggtcca gtgcaggggg tcacgaatgg tcagggagtc
       61 atccgggtac agatggtacc agccctcctc gccgggccgc gcgtcccatg cgaaggggag
      121 cgcctgcagg cggccgccgg ggagctcgac gagatactgc tggagcggcc gcacgccgaa
      181 cgtgtagcgg atctcgaaat cctgcatcga tccgtctggg ccgtcggtgc ggaccaggaa
      241 gcggtcaccg tcccggaaga acgtcgtctc gaggcccgca tgggcgagcg aggacccgtc
      301 gaaccgggcc aggatggtgg acgagtcggc cggctgcatg gcgaggtcgt ggtcggaccc
      361 ggtccaggcg acggtctccg ccgggtgaca gcccgtgcac gcgcctcctc cgacgtaggc
      421 caggggaggc tccgcgcacc ccgcggccgc gaggaggggc aggatcgcca ggcagggagt
      481 cggaaatcgc atgcggcggg cgggcacggg ctcttcgaag atagccacgg cgacgagccg
      541 gatccgatcg cgccccggag cgtgaacatg cccggttccg cattccgtga ccttcatggc
      601 cgagctcaag acccgcccca gcgacgtgag cgtcgagtcc ttcctggact ccgtggccga
      661 cgacgggaag cgtgccgatt cccgtgcact gctcgacctg atgcgcgagg tcaccgggga
      721 ggagcccgtg atgtggggcg gcagcatcgt cgggttcggc gcctaccgct accggtacga
      781 cagcggccga gaaggggact ggttcctgac cggcttcagc ccgcgcaagc agaacctgac
      841 cctttacgtc atggccggct tcgaccgata cgacgagctg atggcccggc tcgggaagca
      901 caagaccgga aagtcgtgcc tgtacctgaa caggctttcg gacgtcgacg ccggcgtgct
      961 ccgcgagctc gtcggccgct cggtcgccca catgaaatcc atgtatcccg catcctgaca
     1021 tgccctggta cgattaccgc tgccgccgct gcggctccgt cttcgagatc cgtcgctcca
     1081 tgaacgagcg ttccgacggc gtttcggtcg agtgctccga gtgcggctcc gcggagacgg
     1141 agcgcgtgtt ccggccgatt gccgtcggcg tggccggctc gcccaggccc gaggcgccgc
     1201 agggggcgtc gtgcgggccg cactgcgcct gccatccgaa ttaggccggc cgcgggaagc
     1261 gtgaacggtc atcggggacc gtaggccccg gccagcctcc gggccacgtt catgagggcc
     1321 cggccgtcgt gataggccga cttccagatg tgcgccttcg ggccgcgggc gagttccggc
     1381 tgccggtcca gaccgccctc gtaccagccg ccgcgctcgt ggtcgaccag gaaggccgac
     1441 acgtaggccc actgctccag gaagcgggcg tggtaggtga gggtatcctc cgggtacagg
     1501 tcgccgagga ggaggagcgt gttcagtccc tccgcctgcg cccaccagtt cttggtgtcc
     1561 cgcagcaccg tcagcgtgga atcgcccgcc aagtagtacc ccgcgtcgat gaagccgccg
     1621 ttctcccggt cccagccgtg gcgcagcgcg tggtccacga gacgcttgcc gacgtccagc
     1681 gaacgcacga cctcgcccac gtcgccgagt gcctccgccg cctccagcac caggtaggcg
     1741 gtctccacgt cgtgcccgaa cgagacgtgg tccaggtact cgttcgcccg gcgcttcgcc
     1801 tcggacgagt cgcggtacga caccggggtc cagtcctcga ggaagaagag ctgcagcgtg
     1861 ccgggggccg tgacgatcgt gtcccggacc aggtgcagca tctcggccag gcgaacccgc
     1921 agcacggagt ccggccagac gcggtagagc tcggtgaagg cttcgagcag gtggatggac
     1981 gagttctggt ccttcggggg cgtggggtcg tagccccggt cggcgggccg gtcggcgagc
     2041 cccgcgcgga gcggggtgcc gtccagctcc aggtagttga agtacccgcc gtgcacgggg
     2101 tcgtgggcgt gcgcgtccag ccagccgaag gcccgccggg cgagcgagag cgccgccgcg
     2161 tcgcccgaca cgtccgcgta tgccgcgaga ccgtagatcg cgaaggcgtt tccgtaagcc
     2221 tgcttcagaa ggcgtccgtc cggctccggc acgggtgcgc cctcgcgggt gaccatccag
     2281 aagaatcccc cgtgctccgc gtcccacatc gagcgggcaa gaaagtccac gccgtgcgcg
     2341 gaacgcggca cccaggtctc cgggtcgccc aggaattccc cggcccgggc ggtggtccag
     2401 acgtgccggg cctgggtcac gatcatcttg tgctggggcc ctgcggcgtt ccagtccgcg
     2461 tcgaagtcgc tcaggtaacc gcccgccacg gtgtcgatcg cgcggggata ccacgcggcc
     2521 atgaggttgg cctcgaagag gcgggccgcc tcgtcgccga gggtgttgcg gcgcccgggc
     2581 ccgagagcct ccggcggctc cggggagcac gccgcggccg agatggcgag gagcaggagg
     2641 gctcgacgca cggccggtca cccctcccgg agcagctcga gcatcccgcg gtccagccgg
     2701 tgcccgcgcc actcctcgta cgccacgtcc tcgcggcccg agtccacgat cccgaacgac
     2761 ccgcgaagat tccacatggc ccatccccag cccacttcct tccagagcga gagcaggtcg
     2821 gccatccacg cgagcgccac gtcgtgcggc gtccggatga agcagcccca ctcgcccacg
     2881 tgcacgggca cgccggccct ggcgagcggt agccacggca cgatgagccg ctcgcggagc
     2941 gtctcccggt tccagaggac gccgttcatg tccaccatcg gccaggacgg gacttcgaac
     3001 gactcgaact cgttttccgg gacccacgtg gcggtgaaat ggctgaccat tttgggcagg
     3061 tagccccgcg tgctctgcac gagtctcagg tccacgaggc ccatcaccgg ggtctggccg
     3121 atgtccgcgc cgtcggccac gatcaggcgg tccgggtcca cctcgcggat cgccccgacc
     3181 agtgcgcgcg cgatctcgat gtaccgcgcg tggtcgtcca tccagggcgg ttcgttgaag
     3241 aggtcgaagc tcaggcgatc gctggggacg ccccggtagc gctcggcgaa gtggcgccag
     3301 tgatgcgtcg cggcctccag ggccatcgcc tgcgactcgg gcggccccgt gaacagctgg
     3361 tgcggctcga gctcggggcc gttcacgcac cagcccggga tccggtggaa gttcaggttg
     3421 acgtgaattc cgtactgccg tccgaaatcg accgcctggt ccacatgggc gagcaccgag
     3481 tcgtcgatcc gcatccagtc gtccgggccg gaccaggacc agtacgacat ggggagccgg
     3541 gcgaagtcga aaccccactc ggccatccac tcgaaatcgg actccaggaa gggccgtacc
     3601 tcgcggcccc cggtgagctc ggtcaggttg aagccgcgcc accgcgggat gacgggctgc
     3661 gtcaccgcgg gagcttccgg ggcggacgtg cggcaggccg ggagcgccag ggcgccgacg
     3721 gcggcggccg atccggtcct gaggaaggtg cggcgatcca tggcgacgga ttcctcagat
     3781 gcatggcgca attgcctttt tagggaccgt gagcgtg
//
LOCUS       DBNHQZ010000416         2845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_529400, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000416 DBNHQZ010000000
VERSION     DBNHQZ010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_529400"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="AC16YU_11295"
     CDS             complement(<1..370)
                     /locus_tag="AC16YU_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065855.1"
                     /translation="MQFLLVGEDGERAGGVRGSLREGDSLRMVPGAGLLTDAGELSVA
                     GLALIESAVRCDAVLFLWDFATAPVLNTLTHAMRDSLAGPSLAVCRPGEAAEALAAGA
                     DAVLVAPFDSADLEAGILAHR"
     gene            467..901
                     /locus_tag="AC16YU_11300"
     CDS             467..901
                     /locus_tag="AC16YU_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065856.1"
                     /translation="MRILVIALMLVLSLPASAQDRVDPGALGGVWRLEFDIDPSSGES
                     AVERIALKLATGFLDEMEIRFSFLEENRLRVMVDAFDTKKEEWSTWRITPEGYLAMGD
                     SDHFDAEDTLWYVDGDRLVAFSSDDREEDGRLKGLALVRILD"
     gene            909..1667
                     /locus_tag="AC16YU_11305"
     CDS             909..1667
                     /locus_tag="AC16YU_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR6065857.1"
                     /translation="MRVPAILLLLTLPLALAACSASREARRARSVGIEWVAIPGGHYL
                     MGDTFERENQDALPLHRVTVAPFRVLRYEVTYRQYDQFAGSTGRRLPDASGTTRGDQA
                     VVDVDWDDASAFCAWIGARLPSEREWEFAAAGGPAKQLYPGTDDPDSVDAYVRHRVNS
                     IGMPGVVGRRRPNVYGLYDMGGNVAEWVGAFYQFYPEDGEEPDWVDLDERGIRILRGG
                     SYSMEPEIARTYWRAGTLREVRSPAIGFRCARGR"
     gene            complement(1714..>2845)
                     /locus_tag="AC16YU_11310"
     CDS             complement(1714..>2845)
                     /locus_tag="AC16YU_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065858.1"
                     /translation="GCLYVPAFEAWALERTSDGTGPVVALSGGGAVSWSRVAALREVR
                     PGDRADRIVRLCPDARIRVRDASVEWACWDGVLDAVNRITPFIAPSRPPWAWFRVDAI
                     ESLRELAAEPGIRIGVARDRATARLAAVRSADRHVLRVAPERRRAFLDRFDVGLLAEL
                     GLPEEMAVRLRLFGYPTLGSLAHLARRQLKAQFGADGEALHDLLHPGEDSAIPLHVPP
                     PAVDVAEEPEAPVRDAELSAVVALLAGRASEGLGDRRTQRIVLEVHPGGGLPRRASRL
                     LPEPHNRADTLRRFALRLLPEVVRPDEAVDRVALRLEALRRPDVSQVDLFSVRPAVVG
                     AVRRVQRRYPGGIRRAVLRPHALLPEDEVRLEAYDDGAPRAA"
BASE COUNT          429 a         1069 c          990 g          357 t
ORIGIN      
        1 ggcggtgcgc gaggatcccg gcctcgaggt cggccgagtc gaagggcgcc acgagcacgg
       61 catcggcacc cgccgccagc gcttccgcgg cctcgccggg gcggcacacg gccagggagg
      121 ggcccgcaag gctgtcgcgc atggcatgcg tgagcgtgtt cagcaccgga gcggtcgcga
      181 aatcccacag gaaaaggacg gcatcgcatc gcacggccga ctcgatgagg gccagccccg
      241 ccaccgacag ctccccggca tcggtgagaa ggcccgcacc ggggaccatc cgaagggaat
      301 ccccttcgcg gagggatccg cgcacgccgc ccgcccgttc gccgtcctca ccgaccagga
      361 gaaactgcat ggtaccgctc gtcgacgcgt gaagatacgc gcgatcgccg aaccgcaacc
      421 gcgagccgct cggcccgata tactggcgtt acccttcagg aaagccatgc gcattctcgt
      481 cattgccctc atgctcgtcc tctcgcttcc cgcctccgcc caggaccgtg tcgatcccgg
      541 cgccctcggc ggagtgtgga gactggaatt cgacatcgat ccctcctcgg gtgaatccgc
      601 cgtcgaacgc atcgctctca agctggccac gggattcctg gacgaaatgg agatccgctt
      661 ctccttcctg gaggagaacc gcctgcgggt gatggtggac gccttcgaca cgaagaagga
      721 ggaatggtcc acctggcgca tcacgcccga gggctacctg gccatgggcg acagcgatca
      781 cttcgacgcg gaggacacgc tctggtacgt ggacggcgac cgcctggtgg ccttctcgag
      841 cgacgacagg gaagaggacg gccggctcaa gggcctggcg ctcgtccgaa tcctcgacta
      901 ggaggggcgt gcgcgttccg gccattctcc tccttctgac ccttccgctc gctctcgcgg
      961 catgttccgc ctcccgcgag gcgaggcgcg ccaggagcgt gggcatcgag tgggtcgcca
     1021 ttcccggcgg ccattacctc atgggcgaca cgttcgagcg cgagaaccag gacgcgctgc
     1081 ccctgcaccg ggtgaccgtc gcgcccttcc gcgtgctccg ctacgaggtc acgtaccgac
     1141 agtacgacca gttcgccggc tccacgggcc gccgcctgcc cgacgcctcc ggcaccacgc
     1201 ggggggacca ggccgtggtc gacgtggact gggacgacgc ctcggccttc tgcgcgtgga
     1261 tcggagcccg tctgccgtcc gagcgggaat gggagttcgc ggcggccggc ggcccggcca
     1321 agcagctcta tcccggcacc gacgatccgg actccgtcga cgcctatgtc cggcaccggg
     1381 tgaacagcat cggcatgccc ggggtcgtcg gacggcggcg accgaacgtg tacggcctgt
     1441 acgacatggg ggggaacgtg gccgaatggg tgggcgcctt ctaccagttc tatcctgagg
     1501 atggagagga gccggactgg gtggacctcg acgagcgggg catccgcatc ctgcgcggcg
     1561 gcagctactc gatggaaccc gagatcgcgc gcacctactg gcgggccggc acgctgcgcg
     1621 aggtccgctc gcccgcgatc ggcttccgct gcgcccgcgg ccggtagacg tccggcgatg
     1681 cgccgtgcgt ggtcccggcg gcgtccccgg acctcaggcc gccctcggcg ccccgtcgtc
     1741 gtaggcctcg agcctgacct cgtcctccgg gagcagggcg tgcgggcgca ggacggcacg
     1801 gcggatgccc cccgggtagc gccgctgcac gcggcgcacg gccccgacga cggcggggcg
     1861 cacggagaag agatccacct gggagacgtc gggccgacga agagcctcca ggcgaagcgc
     1921 cacgcggtcc acggcctcgt ccggccgcac gacctcgggc aggagccgca gggcgaagcg
     1981 ccgcagggtg tccgcccggt tgtggggctc cggcagcagg cgggacgccc gcctcggcag
     2041 gccgccgccg ggatgcacct ccagcacgat gcgctgggtg cgccggtccc ccagcccctc
     2101 cgaggcccgg ccggccagga gggcgacgac cgccgagagc tccgcgtccc gcaccggggc
     2161 ttcgggctcc tccgccacgt cgacggccgg cggcggtacg tgcagcggga tggcggaatc
     2221 ctcgccgggg tgcagcaggt cgtgcagcgc ctccccgtcc gccccgaact gtgccttcag
     2281 ctgccgcctc gccaggtgcg ccagggagcc gagcgtggga tagccgaaca ggcgaagccg
     2341 cacggccatc tcctccggaa ggccgagctc cgcgagcagg cccacgtcga agcggtccag
     2401 gaacgcccgt ctccgctccg gcgccacccg cagcacgtgc cgatccgcgg accggaccgc
     2461 ggccaggcgg gccgtcgccc ggtcccgggc cacgccgatg cggatgcccg gctccgccgc
     2521 gagctccctc agcgactcga tcgcatcgac gcggaaccag gcccacggcg gccgggaagg
     2581 cgcaatgaac ggcgtgatgc ggttcacggc gtccagcacg ccgtcccagc acgcccactc
     2641 cacggacgcg tcccgcaccc ggatccgcgc gtccggacac agccggacga tccggtccgc
     2701 ccggtcgccc ggacgaacct ccctgagcgc cgccacccgg ctccaggaca cggcgccgcc
     2761 cccggagagg gccaccaccg gccccgtccc gtccgacgtc cgctccaggg cccacgcctc
     2821 gaaggccgga acgtacaggc agccc
//
LOCUS       DBNHQZ010000417         6865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_801595, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000417 DBNHQZ010000000
VERSION     DBNHQZ010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_801595"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..2366
                     /gene="ftsH"
                     /locus_tag="AC16YU_11315"
     CDS             255..2366
                     /gene="ftsH"
                     /locus_tag="AC16YU_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6065859.1"
                     /translation="MSDKDRDQIKMDRQGGEGGPRMPDRRPRFALWIYMAIFMALLVH
                     VFLFVSETQPNSVTYSRFLEFIEKGYVAEVVVVNNTRIEGQFTEEAVREGWVPAPPPA
                     QNPFGMGTAEPGTAFVSTKPEDHELVQFILDHNEQARAEGKAIVSIDADQQDNWFGTV
                     LAYLIPLIIIVALWMFLIRRMNPGSQVLNIGKNKAVLFDAMGDQRLTFRDVAGLDEAK
                     EEVVEVVEFLRNPKKFTKLGGKLPKGVLLVGPPGTGKTLLAKAVAGEAGTPFFSLSGS
                     DFVEMFVGVGAARVRDLFRQAKEKAPCIIFIDEIDAIGRTRGRGMMMGANDERENTLN
                     QLLVEMDGFNTDKGVIIMAATNRPDVLDTALLRPGRFDRQILIDKPDRKERAEIFEVH
                     TRELILGLDVELEVLAGQTPGFAGAEIANVCNEAALLAARNGKEAIEMEDFEKAIDRV
                     IAGLEKKNKIISPEEREIVAYHEAGHAITGWFLEYTDPVVKVSIVPRGLAALGYAQYL
                     PEERYLYTKEALLDRMTMAIGGRVAEELVFGRISTGAQNDLERITKLSYAMVVDYGMS
                     EKVGYVSFNMSKEGGESPMFEKPYSDETARIIDQEAKALIDGVRVRARAMLEEKREAL
                     ETMAQALLKKEVLGPRDLVEILGPRPFGEYVQVGNGGSAGSVEAPGPIAGDGSVTEPG
                     GVGEAEGDGARKESESASTSI"
     gene            2573..2947
                     /gene="rpsL"
                     /locus_tag="AC16YU_11320"
     CDS             2573..2947
                     /gene="rpsL"
                     /locus_tag="AC16YU_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR6065860.1"
                     /translation="MPTIQQLVRKGRHDKVRKTKSPALVSCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGIEVIAYIPGEGHNLQEHSIVLVRGGRVKDLPGVKYHIVRGAL
                     DTSGVNDRRQSRSKYGAKRPKN"
     gene            3035..3502
                     /gene="rpsG"
                     /locus_tag="AC16YU_11325"
     CDS             3035..3502
                     /gene="rpsG"
                     /locus_tag="AC16YU_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR6065861.1"
                     /translation="MRKRKAERRLPAPDPVHNDVLVSRFVNAVMHSGKKNVARSLVYQ
                     AIDIAEERSKEEGIEVFRKAVNNVAPLVEVRSRRVGGATYQVPIEVRPERRIALAFRW
                     LIQYARARNDKSMANRLAAELVAAARGEGGAIKKKDDTHRMAEANKAFSHFRF"
     gene            3541..5634
                     /gene="fusA"
                     /locus_tag="AC16YU_11330"
     CDS             3541..5634
                     /gene="fusA"
                     /locus_tag="AC16YU_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6065862.1"
                     /translation="MSSTNGFALERTRNIGIMAHIDAGKTTTTERILFYTGRLHRMGE
                     VHEGGATMDWMEQEKERGITITSAATTCFWADHRINIIDTPGHVDFTIEVERSLRVLD
                     GAVALFCAVGGVEPQSETVWRQADRYRVPRIAFVNKMDRTGADFEAAISQMRERLKAN
                     PIPVQVPIGEGHMFRGVIDLIRNKAIIWHDETQGATWDEIDIPADLQKDARHWRIHLL
                     EAVAEHNDELLLKYLEGEDITPEELKAVVRQATLAMDITPVFCGSAFKNKGVQRLLDG
                     VVDYLPSPLDVPAIRGTSLDGNTEVERHPDPSGPFSAIAFKIATDPYVGKLTFFRVYS
                     GSLDKGSQVLNSSTGKKERVGRILFMHANSREDVDTVRAGDIAAAVGLKEVYTGDTLC
                     DPSKPVVLEQMDFPEPVIRIAIEPKTKADNDKLGLGLQKLAEEDPTFKVNVDHETGQT
                     VIAGMGELHLEIIVDRLRREFKVEANVGRPQVAYREAIQATVDERYQHKKQTGGRGQF
                     AEVWIEIGPNESGVGFEFENGITGGAIPREFIPSVEKGLKSALYQGPLAGYPVEGVKA
                     RLYDGKFHTVDSDQMSFEVAGRMAFRSAARRAKPVIMEPIMSVEVVTPEEYMGDVIGD
                     LNSRRGRIESMGQRQDAQVIKALVPLSNMFGYSTDMRSITQGRAIYSMQFDHYAQVPK
                     SVADEIVEAATGVSA"
     gene            5688..>6865
                     /gene="tuf"
                     /locus_tag="AC16YU_11335"
     CDS             5688..>6865
                     /gene="tuf"
                     /locus_tag="AC16YU_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR6065863.1"
                     /translation="MAKETFQRTKPHVNVGTIGHVDHGKTTLTAAITQVLAKKLGRGE
                     IRSFDSIDNAPEERERGITIATAHVEYETEARHYAHVDCPGHADYVKNMVTGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPYIVVFMNKVDLVDDEELLDLVEMEIRELLS
                     TYEFPGDDIPVVRGSALGALNGEAQWEEKVAELMGAVDSYIPTPQRDMEKPFLMPIED
                     VFSITGRGTVVTGRIERGVVTVGETVDIIGMQEEKQSTTVTGVEMFRKLLDRGEAGDN
                     VGLLLRGTKKDDVERGMVVIKPGSVTPHKEFDCEVYVLSKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGDIELPEGVEMVMPGDNTQFKVKLIVPVAMEQGLRFAIREGGRTVGAGVVS
                     "
BASE COUNT         1319 a         2144 c         2390 g         1012 t
ORIGIN      
        1 gtgctcggac ggttcgactc gcgcggggat gccgagcgtg cggtctcggc tgcgggcgac
       61 ctcgttcccg agggcgcctg gttcctgcac gtgcttcccc gttgagtcgc gcgggaaggg
      121 cccgcgagga accatacagg gggttccggg taacgcgatg cccctccgat cggccggccc
      181 gaaaccagcc cggcgggggt gcgtagcgtt gtatgatgcc tgtcgtccgt gcggcgggcc
      241 acagccggtt ccccatgtcg gacaaggatc gcgaccagat caagatggac cgccagggag
      301 gcgagggcgg cccgcggatg ccggaccggc gaccgcgctt cgccctgtgg atctacatgg
      361 ccatattcat ggccctgctg gtccacgtgt tcctgttcgt cagcgagaca cagccgaact
      421 cggtcacgta ctccagattc ctcgaattca tcgagaaggg atacgtggcg gaggtcgtgg
      481 tcgtcaacaa cacccggatc gagggacagt tcaccgagga ggccgtccgc gagggctggg
      541 tccccgcgcc gccaccggcc cagaatccgt tcgggatggg cacggccgag cccgggacgg
      601 ccttcgtctc cacgaagccg gaggaccacg agctcgtcca gttcatcctg gaccacaacg
      661 agcaggcgcg ggcggagggg aaggccatcg tctcgatcga tgccgaccag caggacaact
      721 ggttcggcac ggtgctggcc tacctgattc cgctcatcat catcgtcgcg ctctggatgt
      781 tcctcatccg gcgcatgaac ccgggctccc aggtcctgaa catcggcaag aacaaggccg
      841 tcctcttcga cgcgatgggt gaccagcggc tcacgttccg cgacgtggcg ggcctggacg
      901 aggcgaagga ggaggtcgtc gaagtcgtcg agttcctgag gaatccgaag aagttcacga
      961 agctcggggg caagctgccc aagggtgtgc tcctcgtcgg gccgcccggc acgggcaaga
     1021 cgctcctggc gaaggcggtc gccggcgagg ccgggacccc gttcttctcg ctctcgggat
     1081 cggattttgt cgagatgttc gtgggtgtgg gcgcggcccg ggttcgcgac ctgttccgcc
     1141 aggccaagga aaaggcaccg tgcatcatct tcatcgacga gatcgacgcc atcggccgga
     1201 cgcgtggccg cggcatgatg atgggcgcga acgatgagcg cgagaacacg ctcaaccagc
     1261 tgctcgtcga gatggacggc ttcaacacgg acaagggcgt catcatcatg gccgccacca
     1321 accggccgga tgtcctggat acggcgctgc ttcgtcccgg ccggttcgac cggcagatcc
     1381 tgatcgacaa gccggaccgc aaggaacggg cggagatctt cgaggtccac acgcgcgagc
     1441 tgatcctggg cctggacgtc gagctcgaag tgctggccgg ccagacgccg ggattcgccg
     1501 gggcggagat cgccaacgtg tgcaacgagg ccgcccttct cgctgcccgc aacggcaagg
     1561 aagcgatcga gatggaggac ttcgaaaagg cgatcgaccg ggtgatcgcc gggctcgaga
     1621 agaagaacaa aatcatctct cccgaagagc gcgagatcgt cgcctaccac gaggccggcc
     1681 acgccatcac ggggtggttc ctggagtaca ccgaccccgt cgtgaaggtg tcgatcgttc
     1741 cgcgaggcct ggccgcgctc gggtacgcgc agtacctgcc cgaggagcgg tacctttata
     1801 ccaaggaggc gctcctggac cggatgacga tggccatcgg cgggcgcgtg gccgaggagc
     1861 tcgtgttcgg ccgcatatcg accggcgcgc agaacgacct cgagcgcatc accaagctct
     1921 cgtacgcgat ggtggtggac tacggcatga gcgagaaggt cgggtatgtc tcgttcaaca
     1981 tgtcgaagga gggcggcgag agcccgatgt tcgagaagcc gtattcggac gagaccgccc
     2041 ggatcatcga ccaggaggcc aaggccctga tcgacggagt ccgggtgcgg gcccgcgcta
     2101 tgctcgagga aaagagggaa gcgctggaga ccatggcgca ggccctgctc aagaaggaag
     2161 tgctcggccc ccgtgacctg gtggagatcc tgggcccgcg gccgttcggc gagtacgtgc
     2221 aggtgggcaa cggcggctcg gcgggatccg tggaagcccc cgggccgatc gccggagacg
     2281 ggtccgtgac ggagcccggc ggcgtcggtg aggccgaggg ggacggtgca cggaaggaat
     2341 ccgaatcggc cagtacttcg atatagtgat atagggcagt atatcgtact gctctcgatc
     2401 cttgctgccg gattcggtca gggtgcgcct gcgtggcttc tcatcgccct ttcttcacgt
     2461 gaacactggc ttggttcgac tgaatcggag tatattaaca ggctgaaagt acgcgcgcgc
     2521 agacgtgcgt gcgatttcac cgatcaacga cctgccaaga aaggaagcgc tcgtgccaac
     2581 gattcagcag ctagtccgca aggggcgcca cgacaaggtg cggaagacga agtctcccgc
     2641 cctcgtgtcg tgcccgcagc gccggggcgt ctgcacgcgt gtgtacacca ccacgcccaa
     2701 gaagccgaac tccgcgctgc gcaaagtcgc caaggtgcgt ctcacgaacg gcatcgaggt
     2761 catcgcctac atcccgggcg agggccacaa cctgcaggag cactccatcg tgctcgtgcg
     2821 cggcggccgc gtcaaggacc tgcccggcgt caagtaccac atcgtgcgag gcgccctgga
     2881 tacttccggg gtcaacgacc gccgccagag ccgctccaag tacggagcca agcgacccaa
     2941 gaactagcac cctgcccttc ggggacgcga gccccgaagc cgtcggagga ccgatgcgga
     3001 agcgggcctc cggtaaccgg acgaaagcga gaccatgcgt aagagaaaag cggaacggag
     3061 actgccggcg cccgacccgg tgcacaacga cgtccttgtg tcccggttcg tgaacgcggt
     3121 gatgcacagc ggcaagaaga acgtggcgcg cagcctcgtg taccaggcga tcgacatcgc
     3181 cgaggagcgc tcgaaggagg aaggcatcga ggtcttccgc aaggccgtga acaacgtcgc
     3241 tcccctggtg gaggtgcgca gccggcgggt gggcggcgcc acgtaccagg tgccgatcga
     3301 ggttcgtccc gaacggcgga tcgcgctggc gttccgctgg ctcatccagt acgcacgcgc
     3361 ccgcaacgac aagagcatgg ccaaccgcct ggcggccgag ctcgtcgccg ccgcgcgcgg
     3421 agagggaggc gcaatcaaga agaaggacga cacccaccgc atggcggagg cgaacaaggc
     3481 cttctcgcac ttccgcttct aggaccgcgc ccaatcgcaa cggccattcc cgacgatccc
     3541 atgtcttcca ccaacggctt cgcgctggag cgcacgcgca acatcggcat catggcccac
     3601 atcgatgccg ggaagacgac gaccaccgag cgcatcctgt tctacacggg acgccttcac
     3661 cgcatgggcg aagtccatga gggcggcgcc accatggact ggatggagca ggagaaggag
     3721 cgcggcatca cgatcacgag cgcggccacc acgtgcttct gggcggacca ccggatcaac
     3781 atcatcgaca cgcccggcca cgtggacttc acgatcgagg tggagcgctc gctccgcgtc
     3841 ctcgacgggg ccgtcgcgct gttctgcgcc gtgggcggcg tggagccgca gtcggagacc
     3901 gtgtggcgcc aggcggaccg ctaccgcgtt ccgcgcatcg cattcgtcaa caagatggac
     3961 cgcacgggcg ccgatttcga ggccgccatc agccagatgc gtgagcgcct gaaggccaac
     4021 cccatcccgg tgcaggtgcc catcggcgag ggccacatgt tccgtggcgt gatcgacctc
     4081 atccgcaaca aggcgatcat ctggcacgac gagacgcagg gcgccacgtg ggacgagatc
     4141 gacattcccg ccgacctcca gaaggacgcg cggcactggc gcatccacct gctcgaggcg
     4201 gtggccgagc acaacgacga gctcctgctc aagtacctgg agggcgagga catcacgccc
     4261 gaggaactga aggcggtcgt ccggcaggcc acgctcgcga tggacatcac gccggtcttc
     4321 tgcggctcgg ccttcaagaa caagggcgtc cagcgccttc tcgacggggt cgtcgactac
     4381 cttcccagcc cgctcgacgt gccggccatc aggggcacgt cgctcgacgg gaacacggag
     4441 gtcgagcggc atcccgatcc gtcgggtccc ttcagcgcga tcgccttcaa gatcgcgacg
     4501 gatccgtacg tcgggaagct gacgttcttc cgcgtctact cgggctcgct ggacaagggc
     4561 agccaggtgc tgaactcgag cacgggcaag aaggagcgcg tgggccgcat tctcttcatg
     4621 cacgccaaca gccgcgaaga cgtggatacg gtccgcgccg gcgacatcgc cgcggccgtg
     4681 gggctcaagg aggtctatac cggcgacacg ctgtgcgatc cgtccaagcc ggtggtcctc
     4741 gagcagatgg acttccccga gcccgtcatc cggatcgcca tcgagcccaa gaccaaggcg
     4801 gacaatgaca agctgggcct gggcctgcag aagctggccg aggaggaccc caccttcaag
     4861 gtgaacgtgg accacgagac cggccagacc gtcatcgccg ggatgggcga gctccacctc
     4921 gagatcatcg tcgaccggct ccgccgcgag ttcaaggtcg aggccaacgt gggccgcccg
     4981 caggtcgcct atcgcgaggc catccaggcg accgtcgacg agcgctacca gcacaagaag
     5041 cagacgggcg gccgcggcca gttcgccgag gtctggatcg agatcggacc gaacgagtcc
     5101 ggagtcggat tcgagttcga gaacgggatc accggaggcg ccatcccgag ggagttcatc
     5161 ccctcggtcg agaagggcct caagtcggcg ctgtaccagg gcccgctcgc cgggtatccg
     5221 gtcgagggcg tgaaggcccg cctgtacgac ggcaagttcc acacggtcga ctcggaccag
     5281 atgtcgttcg aggtggccgg gcgcatggcc ttccgcagcg cggcacggcg cgccaagcca
     5341 gtcatcatgg agccgatcat gtcggtcgag gtcgtcacgc ccgaggagta catgggcgac
     5401 gtcatcggcg acctgaacag ccgccgcggg cggatcgaga gcatgggcca gcggcaggac
     5461 gcgcaggtca tcaaggccct cgttccgctt tcgaacatgt tcggttactc gaccgacatg
     5521 cgctcgatca cgcagggacg cgcgatctac agcatgcagt tcgaccacta tgcccaggtg
     5581 cccaagagcg tggccgacga gatcgtcgag gccgcgaccg gcgtcagcgc ctgacccgca
     5641 cctacaaccg aaagaaccag aatccagcca aatccggagc agcagccatg gccaaggaga
     5701 cattccagcg tacgaagccc cacgtgaacg tgggaacgat cggacacgtg gaccacggta
     5761 agacgacatt gacggcggcg atcacgcagg tcctggcgaa gaagctgggg cggggcgaga
     5821 tccgctcgtt cgactcgatc gacaacgcac ccgaggagcg cgagcgcggg atcacgatcg
     5881 cgacggctca cgtcgagtac gagacggagg cgcgccacta cgcgcacgtg gactgtccgg
     5941 gccacgcgga ctacgtgaag aacatggtga cgggggcggc tcagatggac ggtgcgatcc
     6001 tggtggtggc ggcgaccgac ggtccgatgc ctcagacgcg ggagcacatc ctgttggcgc
     6061 gccaggtggg ggtgccctac atcgtggtgt tcatgaacaa ggtggacctg gtcgacgacg
     6121 aggagctttt ggacctggtg gagatggaga tccgggagct cctgtcgacg tacgagttcc
     6181 cgggcgacga catcccggtg gtgcggggct cggcgctggg agcgctcaac ggcgaggcgc
     6241 agtgggagga gaaggtggcc gagctcatgg gcgcggtgga ctcgtacata ccgacgccgc
     6301 agcgggacat ggagaagccg ttcctgatgc cgatcgagga cgtgttctcg atcacgggcc
     6361 ggggtacggt ggtgacgggc cggatcgagc gcggggtggt gacggtcggg gagacggtgg
     6421 acatcatcgg gatgcaggag gagaagcagt cgacgacggt gacgggggtg gagatgttca
     6481 ggaagctcct ggaccgtggc gaggcggggg acaacgtggg gctgctcctg cggggcacga
     6541 agaaggacga cgtggagcgg ggtatggtgg tgatcaagcc ggggagcgtg acgccgcaca
     6601 aggaattcga ctgcgaggtg tacgtgctct cgaaggaaga gggaggccgt cacacgccgt
     6661 tcttcaaggg gtaccgtccc cagttctact tccggacgac cgacgtgacg ggcgacatcg
     6721 agctgcccga gggcgtcgag atggtgatgc cgggcgacaa cacgcagttc aaggtgaagc
     6781 tgatcgtgcc ggtggcgatg gagcaggggc tgcggttcgc catccgcgag ggtggccgga
     6841 cggtcggcgc cggcgtcgtg tccaa
//
LOCUS       DBNHQZ010000418         6714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3377131, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000418 DBNHQZ010000000
VERSION     DBNHQZ010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3377131"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="AC16YU_11340"
     CDS             complement(<1..887)
                     /locus_tag="AC16YU_11340"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6065864.1"
                     /translation="MTGLQLAQYRIDAELGRGGMGIVYRATDTKLDRTVALKVLPASA
                     LSSEDDRARFYREAKAAAQLHHAHIATVFEIGEAVPEGADAGPSDGPRPYIAMEFIDG
                     STLSARIARGPFKLPEAVRIGLEIADALKAAHAKGIVHRDIKSANIMLTADGTAKVLD
                     FGLAKTAASTQLTRQGSTLGTVAYMSPEQARGEEVDGRTDLYALGTVMYEMVAGRLPF
                     AGDYEQAILYAILNSPPEPLTAVRTGVPNGLEFVVAKCLSKDRELRYQTAADLVADLK
                     SLSLGGSGFSRVESMHSSAS"
     gene            1128..3116
                     /locus_tag="AC16YU_11345"
     CDS             1128..3116
                     /locus_tag="AC16YU_11345"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6065865.1"
                     /translation="MKIRVVVAIALFATLAPASVATAQDDVPRAIRRDVPMTNAIRRA
                     HDAGTRDRTGRPGPAYWQLRTDYEIDARLDPSTQTISGTETITLHNDSPDSLDRIVLR
                     LDHNIFRDDVPRGFSVPAEETEGMVVTRLIVNGREIDLYARPEPRFGRNDPRNGITGL
                     TQTVAAIGLEEPIPPGATATVDVEWHTRLPGGPNGRGHRMTQRWEDYLFQPTQWFPRI
                     AKYDDLRGWDTNVYLGPAEFYNNFGRFDVRIDVPGGWIVSGTGVLQNPEEVLTDTARE
                     RLATVLESDAEVTVVAENEPKTADGASLVWHFVADQVNDFAWAASPRFVWKATRATIP
                     SRGPIPIHMVHLPDRARYFERAGEITRHALEFYSALWAPYPFPQLTLQDGPSSGMEYP
                     MVINSNQGAADHETAHQWWPMMVGNNETWYGWMDEGFNVYMNVLSGADSRGEAPQLDG
                     LGQQYGQIAGLEDEPPLMWNANYGGPLYGFQTYQKTSMMLSMLGGIVGDEAVQEAMRA
                     YTAEWSFRHPSPWDYAFLVERVLGVDLGWFWYYWLWTTESVDGSIESVDRDGERTSVT
                     VRQDGGMPSPVVLRVELAADASTTMPNARMIDARTAVVTWPVDVWFDGRRTFEAVLDF
                     GPAEVARVVFDPQCRFPDRNPSDNVWPATGSDGSWCAQ"
     gene            3212..5068
                     /locus_tag="AC16YU_11350"
     CDS             3212..5068
                     /locus_tag="AC16YU_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR6065866.1"
                     /translation="MPRALLLVVALCATTASAQPYLLPAGYDSGLPRVAANDNRTPGG
                     ALRDGVLEIDLELVEADFRPETDDRPGVRVLALREVGGLPMIPAPLVRVEAGVRVRAR
                     ITNTLADSTIHVFGWHARPAGSPDSLTVRPGNTGMVEFEAGEPGTYFYQVRVGSAPTV
                     AFGPETEQLIGALVVDPPGGSPPDRVLVINIWSRDVPEEEAESGYLEVLTINGKSWPH
                     TELQRPEVGELQRWRVVNGSVRNHPMHLHGFYFDVTDRAGLLASEEYADRDRRTVVTE
                     FMRSFTTMDMEWTPTRPGTWVFHCHLSFHVSAETRLPGADALDGHGHHPHMAGLVLGI
                     EIPPGPTDLVERHEAAHLELHALEYADTDSTTRFAFSFDPSFVPDSTRPATPGPLLLM
                     RQYQPTWVTVRNHLGVPTGVHWHGLELDAWADGVPGWSASDGKMSPVIAPDSSFTYKL
                     SAMRPGTFIYHTHLDDIDQLTRGLYGPLIVLGEHDSFDPATDHVNVVGWRKPDPGGMD
                     DVEMNGTTAQPEVRARVGETHRIRVINIAPAGNIGVHMTSGDEPVPLVPVAKDGADLP
                     AHQQVPAEQSGRFGVGETADFEFTPTEPGTYTLHIGYAPSAHWRQTWTVTDR"
     gene            5096..>6714
                     /locus_tag="AC16YU_11355"
     CDS             5096..>6714
                     /locus_tag="AC16YU_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6065867.1"
                     /translation="MSGVRSLSCMPTIDPSPAPSRGSDPMIGRQVNQYRIVERLGEGG
                     MGLVYKAEDTSLGRTVALKFLPRTFAANSEFEARFMREARTASALDHPNIGVIHEIAR
                     SDQGELYIAMAYYGGETLERKIEDGPLPVDEALEYAIQIARGLERAHDEGIIHRDIKP
                     GNVIFTDRGLAKIVDFGLARMEDVTMTRDGTSLGTVAYMSPEQAHGTGVDRRTDLWAL
                     GVVVYEMLAGRRPFAGGYEAAILYAAAHEPHVSLSALRPEVPEVVSVIVDGLLAKDPE
                     SRYQSATDVIGALEAARVGNAAPGATLSGLMPSVAVPAAAAAPARDRRRPAIVAGASV
                     LGLAAVAVAAWLLFGSGGGPSVSPEARERARAEHDTALEMLSDGRFSVAQARLERAVQ
                     LDPEYSAAWSTLAGLHIQTREYLQAVQAGERAVAIDSTNSVAWYNLGYALQETGAPEE
                     AADAYRRAIRADRNLQQAYSAMGDVLIELGRASEALTVLDEGIRTAPESDIRFLLDKN
                     MGKALLALESPGEALEHLERSLAAHADWPETL"
BASE COUNT         1050 a         2384 c         2284 g          996 t
ORIGIN      
        1 gacgctgacg aatgcatcga ttccacccgg gagaagccag agcctcccag gctcagggac
       61 ttcaggtcgg ccaccaggtc tgcggccgtc tggtaccgga gctcacggtc cttcgagagg
      121 cacttcgcca cgacgaactc gagtccgttc ggcacgccgg tgcgtacggc ggtgagcggt
      181 tcaggcgggg aattgagtat cgcgtacagg atcgcctgct cgtaatcgcc ggcgaacggc
      241 agccggcccg cgaccatctc gtacatcacg gtgcccagag cgtacaggtc ggtgcggcca
      301 tcgacttctt ccccgcgcgc ctgctcgggc gacatgtacg ccacggtgcc gagcgtcgag
      361 ccctgccgcg tgagctgcgt cgaggccgcc gtcttcgcca gcccgaaatc cagcaccttg
      421 gccgtgccgt cggccgtcag catgatgttc gccgacttga tgtcccggtg aacgatgccc
      481 ttggcatgcg ccgccttgag cgcatccgcg atttcgaggc cgatgcggac ggcttccggg
      541 agcttgaagg gtcccctcgc gatgcgcgcc gagagcgtcg aaccgtcgat gaactccatt
      601 gcgatgtacg ggcgcgggcc gtcggagggg ccggcatcgg cgccttcggg caccgcctct
      661 ccgatctcga acaccgtagc gatatgcgcg tgatgaagct gtgcagccgc cttggcctcc
      721 cggtagaaac gggcacgatc gtcctcggac gagagggccg aagcgggcag gaccttgagc
      781 gcgaccgtcc ggtcgagctt cgtgtcggtc gcgcggtaca cgatgcccat tccgccacgg
      841 ccgagctcgg cgtcgatgcg gtactgggcc agttggagac cggtcatatg ggggtcgacc
      901 gaatggggga agcagcactg aagaaacgtc gcccgcccgg gcttcacaac tcctttgaat
      961 cacgacccgg ggcggcgtcc gccggaacat tcgcgtggcc gccccgacgc ccgtggacga
     1021 tttccggatg agcgggccag gctcctcttc cgcccctccc ttgcgatccc cgtccgaggg
     1081 ctgtaccgtc ggatgcctgc cgtgtccatc cgaccaaccg ccccgccatg aagatccgcg
     1141 tcgtagtcgc catagccctc ttcgccacgc tcgcgcccgc ctctgtcgcc acggcccagg
     1201 acgacgtccc ccgcgccatc cgccgcgacg tcccgatgac gaatgccatc cggcgggcgc
     1261 acgacgccgg cacccgggac cgcacaggcc gtccggggcc cgcctactgg cagcttcgca
     1321 cggactacga gatcgacgcc cgcctcgatc catccacgca gaccatctcg ggcaccgaga
     1381 cgatcacgct tcacaacgac agcccggatt cgctcgaccg catcgtgctt cggctggatc
     1441 acaacatctt ccgggacgac gtgccgcggg gcttctcggt cccggccgaa gagaccgagg
     1501 ggatggtggt cacccgcctt atcgtgaacg gccgcgagat cgacctctac gcgcggcccg
     1561 agccccgatt cggccgcaac gatccgcgga acgggatcac gggcctcacc cagacggtgg
     1621 ccgcgatcgg cctggaggag ccgatcccgc cgggcgccac cgcgaccgtc gacgtcgagt
     1681 ggcacaccag gctcccgggc gggcccaacg gccgcggaca ccgcatgacg cagcgctggg
     1741 aggattacct gttccagccc acgcagtggt tcccgcgcat cgcgaagtac gatgacctgc
     1801 gcggatggga caccaacgtg tacctggggc cggccgagtt ctacaacaac ttcgggcgct
     1861 tcgacgtccg catcgatgtc cccggcggct ggatcgtgag cggcacgggc gttctgcaga
     1921 atccggaaga ggtgctcacc gacacggccc gggagcgcct ggcgaccgtc ctggaatcgg
     1981 acgccgaggt caccgtcgtc gccgagaacg agccgaagac ggccgacggc gcctcgcttg
     2041 tctggcactt cgtcgcggac caggtgaacg acttcgcgtg ggcggcgtcg ccccgcttcg
     2101 tgtggaaggc gacgcgcgcc acgattcctt cccgtggccc gatcccgatc cacatggtcc
     2161 acctgcctga ccgggcgcgg tacttcgagc gcgcgggaga gatcacgcgg cacgccctcg
     2221 agttctattc cgccctgtgg gcgccatacc ccttcccgca gctcaccctt caggacggac
     2281 cctcgtccgg catggagtac cccatggtca tcaactcgaa ccagggcgcc gcagaccacg
     2341 agaccgccca ccagtggtgg cccatgatgg tcggcaacaa cgagacctgg tacggctgga
     2401 tggacgaggg cttcaacgtg tacatgaacg ttctctccgg tgcggactcg cgcggcgagg
     2461 cgccgcagct cgacggcctc ggccagcagt acgggcagat cgccggcctg gaggacgagc
     2521 cgcccttgat gtggaacgcc aactacggcg gccccctgta cggcttccag acctaccaga
     2581 agacgtccat gatgctctcg atgctcggcg ggatcgttgg tgacgaggcg gtgcaggaag
     2641 cgatgcgcgc gtacacggcc gagtggtcct tccggcatcc ttcgccgtgg gattacgcct
     2701 tcctcgtgga gcgggtgctc ggcgtggacc tcggctggtt ctggtactac tggctctgga
     2761 ccacggagtc ggtggacggg tcgatcgagt ccgtggacag ggacggcgaa cggacttccg
     2821 tgacggtgcg ccaggacggc ggcatgccgt cgccggtcgt gctccgcgtg gagctcgccg
     2881 cggacgcatc gaccacgatg ccgaacgccc ggatgatcga cgcgcggacg gccgtcgtga
     2941 cctggcccgt cgacgtctgg ttcgacggcc ggcggacgtt cgaggccgtg ctcgacttcg
     3001 gaccggccga ggtcgcgcgg gtcgtcttcg acccgcagtg ccgattcccg gatcgcaatc
     3061 cgtcggacaa cgtctggccg gcgacgggaa gcgacgggag ctggtgcgcg caataaactg
     3121 tgcgacaggg ccttcgcggc gatgggggac gtcatatgtc cgccggggaa ttcctatact
     3181 ccggccgctc ccccctccag gaccccccta gatgccccga gccctgctgc tcgtcgttgc
     3241 gctttgtgcc acgacggcga gcgcccagcc gtacctcctc ccggccggct acgattccgg
     3301 ccttccgcgc gtagccgcga acgacaacag gacgcccggc ggcgctctcc gggacggcgt
     3361 gctggagatc gatctcgagc tggtcgaagc cgatttccga cccgagaccg acgaccgtcc
     3421 gggcgttcgc gtgctcgcgc tgcgcgaggt cggcggcctt ccgatgatcc cggcgccgct
     3481 ggtccgggtc gaggccggcg tccgtgtgcg cgcccggatc acgaacaccc tcgccgactc
     3541 gacgatccac gtcttcggct ggcacgcccg gccggcgggg tcgccggaca gcctcacggt
     3601 ccgtccgggc aacacgggca tggtggagtt cgaggccggc gagcccggca cgtacttcta
     3661 ccaggtccgc gtcgggagcg cgccgacggt cgccttcgga ccggaaacgg agcagctgat
     3721 cggtgcgctg gtcgtggatc cgccgggcgg ctccccgccg gaccgcgtcc tggtgatcaa
     3781 catctggtcg cgggacgtgc cggaggagga ggccgagtcg gggtacctcg aggtcctcac
     3841 gatcaacgga aagtcctggc cccacaccga gctgcagcgc cccgaggtcg gtgagctcca
     3901 gcggtggcgg gtcgtgaacg gctccgtgcg caatcacccg atgcacctgc acggcttcta
     3961 cttcgacgtg accgatcgcg ccggcctgct ggccagcgag gagtatgccg acagggatcg
     4021 ccgcacggtc gtcaccgagt tcatgcggag cttcacgacg atggacatgg agtggacccc
     4081 gacgcgaccc gggacctggg tgttccactg ccacctctcc ttccacgtgt cggccgagac
     4141 gcgccttccg ggcgccgacg cgctcgacgg gcacggccac catccgcaca tggccggcct
     4201 ggtcctcggc atcgaaattc cgcccggacc cacggacctc gtcgagcgtc acgaggcggc
     4261 ccacctcgag ctccacgcac tcgagtatgc cgatacggat tcgaccacgc ggttcgcgtt
     4321 ctcgttcgat ccgtcgttcg tgccggactc cacgcgcccg gcgacgcccg ggccactgct
     4381 cctgatgcgc cagtaccagc cgacgtgggt caccgtccgc aaccacctgg gcgtcccgac
     4441 cggcgtgcac tggcacggcc tggagctgga cgcgtgggcg gacggcgtgc cgggatggag
     4501 cgcgtccgac ggcaagatgt cgccggtcat cgcgcccgac tcgtccttca cctacaagct
     4561 ctcggccatg cggcccggaa cgttcatcta ccatacgcac ctggacgaca tcgaccagct
     4621 gacgcgcggc ctgtacggtc ccctcatcgt gcttggcgag cacgattcgt tcgatcccgc
     4681 gaccgaccac gtgaacgtcg tgggatggcg caagccggat ccgggcggca tggacgacgt
     4741 cgagatgaac ggcacgaccg cgcagcccga ggtgcgcgcg agggtcgggg aaacccaccg
     4801 catccgcgtg atcaacatcg cgcccgccgg caacatcggc gtccacatga cgtcgggcga
     4861 cgagccggtg ccgctggtcc ccgtcgccaa ggacggggcc gacctgcccg cccaccagca
     4921 ggtgccggca gaacagagcg gccggttcgg cgtcggagag acggccgact tcgagttcac
     4981 gcccaccgag cccggcactt acacgctgca catcggctac gcgcccagcg cccactggcg
     5041 gcagacctgg acggtgaccg accggtaggg atcttcccgc ggcgtacggc gcttcatgtc
     5101 cggcgtccgg agcctgtcct gcatgcccac gatcgacccc tcgcccgcac cctcccgcgg
     5161 ctccgatccg atgatcggcc gccaggtgaa ccagtaccgc atcgtcgaac gcctgggcga
     5221 aggcgggatg ggcctcgtgt acaaggccga ggacacctcg ctcgggcgga cggtcgccct
     5281 gaagttcctg ccccggacgt tcgcggccaa ttccgagttc gaggcccggt tcatgcgcga
     5341 ggcgcggacc gccagcgcgc tcgaccatcc gaacatcggc gtgatccacg agatcgcgcg
     5401 cagcgaccag ggcgagctgt acatcgccat ggcgtactac ggcggcgaga ccctcgagcg
     5461 caagatcgag gacggcccgc tgccggtgga cgaggcgctc gaatacgcca tccagatcgc
     5521 ccgcggcctg gagcgggcac acgacgaggg catcatccac cgggacatca agcccggcaa
     5581 cgtcatcttc accgaccgcg gcctggcgaa gatcgtggac ttcggcctgg cccggatgga
     5641 agacgtcacg atgacccggg acggcacgtc gctcggcacg gtcgcgtaca tgagccccga
     5701 gcaggcccac ggcacgggag tcgaccggcg gacggacctc tgggcgctgg gcgtcgtggt
     5761 ctacgagatg ctcgcgggcc gcaggccctt cgccgggggc tacgaggccg cgatcctcta
     5821 cgccgcggcg cacgaacccc atgtgtcgct ttcggccctg cgccccgagg tgccggaagt
     5881 ggtttcggtg atcgtggacg ggctcctggc caaggatccg gagagccggt accagtcggc
     5941 gaccgacgtg atcggcgccc tcgaggccgc ccgcgtgggg aacgccgctc caggcgccac
     6001 gctttcgggc ctgatgccga gcgttgccgt ccccgcggcc gcggccgcgc cggcgcggga
     6061 tcgccgccgc ccggcgatcg ttgcgggggc ctccgtgctg ggcctcgctg ccgtcgcggt
     6121 ggccgcgtgg ctcctcttcg gaagcggagg cggtccgtcg gtcagtccgg aggcgcgcga
     6181 gcgggcgcgc gccgagcacg acacggcgct cgagatgctt tccgacggac gattctccgt
     6241 cgcgcaggcc cgcctggaac gggccgtgca actcgatccg gaatactcgg cagcgtggtc
     6301 cacgctggcc ggcctgcaca tccagacgcg ggaatacctg caggcggtcc aggccggaga
     6361 gcgcgcggtc gcgatcgact cgacgaacag cgtcgcctgg tacaacctgg gctacgccct
     6421 gcaggaaacg ggcgcgcccg aggaggcggc cgacgcgtac cgccgggcga tccgggccga
     6481 ccggaacctg cagcaggcct acagtgcgat gggcgacgtg ctcatcgagc tgggccgggc
     6541 gagcgaggcg ttgacggtgc tggacgaggg cattcggacg gcgcccgagt cggacatccg
     6601 gttcctgctc gacaagaaca tgggcaaggc gctgctcgcc ctcgagagcc cgggcgaggc
     6661 cctggagcac ctggagcgct cgcttgcggc gcacgccgac tggcccgaga cgct
//
LOCUS       DBNHQZ010000419         5226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1076986, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000419 DBNHQZ010000000
VERSION     DBNHQZ010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1076986"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="AC16YU_11360"
     CDS             complement(<1..741)
                     /locus_tag="AC16YU_11360"
                     /inference="COORDINATES: protein motif:HMM:NF016146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease I family protein"
                     /protein_id="HIR6065868.1"
                     /translation="MHPRLHGILALAAFVAALAAPAALAQTCPPPLGDSPGMLRGRYA
                     PRRVLSYNEGRDTLYAHWAEARGGTIRTVYTGREVPLTEGDPTQVLYRQGVNTEHSWP
                     RSRGSDGYPAEADLHMLFPSDERTNSRRGNLPYGEVSDALVQTWAVGGDGFRGPPPGD
                     ADYVSKIGAEAFEPPDPVKGDLARAHFYFALMYPDRVDALFFERELPDLLAWHLADPP
                     SREEMRRDSVIQAIQGNCNPFVRWPDLVG"
     gene            925..1410
                     /locus_tag="AC16YU_11365"
     CDS             925..1410
                     /locus_tag="AC16YU_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6065869.1"
                     /translation="MARMLAAVFCLALAVAPLPLAAQGLTQAESDAIEDTVGRLFAEI
                     TEATNALDLERLLALHEDGDELTYVAAGRVTRSHEAFAGLLQAQLGGLSAADVRLLDP
                     SVDVLARDVALATSTYEFTATFPSGSRFTTTGTYMAVFVLRDGGWKIRYSSHTFPQRE
                     P"
     gene            complement(1454..2029)
                     /locus_tag="AC16YU_11370"
     CDS             complement(1454..2029)
                     /locus_tag="AC16YU_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065870.1"
                     /translation="MKRILLLAALTVAFVGTASAQYSTRGFFVRPHLVGAAWRLDDFE
                     EDAEAGGGLGLALGYGFNERVAAYLEFSGASIDPDTGNDYGLGHFDLGAMFTVGSYAS
                     KLKGNFMVALNGRAAELDQPGADIEASGAGLTLGGGVLYFTSPNLALDLNLLFTVGEI
                     SEVRVNGLTWNTNLDATSTRLNLGVAWFPGR"
     gene            complement(2415..3293)
                     /locus_tag="AC16YU_11375"
     CDS             complement(2415..3293)
                     /locus_tag="AC16YU_11375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6065871.1"
                     /translation="MHPIARSRVPGVNDRPDGDMAALSETLIDRLEQAEAEAWIDVCE
                     ADAIRTDGFGWAAWLTEDATIFCAPRSNVLFHNRAIGLWDERADEAAIDRVVDLFRTA
                     RAPRFFVHVSPRAEARGLPEALAERGFEHHNDWLKLYRTVDDPPEEEPDAHITRLPSR
                     LGPRYGLDVTRAFDWPIGLAETIGATPDRPGWKVYVAWIDAEPAAYGGLYVCGGSAYL
                     GPAFTREEFRRRGAQTALIKHRIREAARHGCALLVTETADDTPEKPNPSTRNLMSAGF
                     ELAYRRANWRMDLATH"
     gene            complement(3356..4417)
                     /gene="arsB"
                     /locus_tag="AC16YU_11380"
     CDS             complement(3356..4417)
                     /gene="arsB"
                     /locus_tag="AC16YU_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIR6065872.1"
                     /translation="MPHAVTRRLSTLDRFLTLWIFGAMAVGVGLGALLPGVEGFIDRF
                     QVGTTNIPIAVGLILMMYPPLAKVRYEKFGAVLRHGRVLALSLVQNWIVGPLVMFGLA
                     MAFLPDMPGYAVGLILVGLARCIAMVLVWNDLADGDRDLAAGLVAANAVFQVLLYGVY
                     AWFFITLLPPFFGMEGAIVDVSIGEIAGTVLLYLGVPFAAGLVTRLVLLRLKGDAWYE
                     TTFIPRISPVTLVALLFTIVVMFSLKGELIVELPFDVLRVAVPLVLYFVIMFLITFFA
                     ARAMRGRYPEVTTVALTAASNDFELAIAVAVAVFGIGSAEAFATVIGPLVEVPVLIGL
                     VQVSLFFRRRFFPEAVAGS"
     gene            complement(4497..4856)
                     /locus_tag="AC16YU_11385"
     CDS             complement(4497..4856)
                     /locus_tag="AC16YU_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6065873.1"
                     /translation="MTDEQRSASCSPDPGRARVLLPEADLEFAAGMMKALGHPVRLRM
                     AELIHAGGGDLCVCEIERDFDLTQPTITHHLKVLREAGVIRSRKEGLWVRHAVEPAAF
                     EHLRALVHTLADLPEPA"
     gene            5041..>5226
                     /locus_tag="AC16YU_11390"
     CDS             5041..>5226
                     /locus_tag="AC16YU_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR6065874.1"
                     /translation="MISQASRYALRALAYLATHDTERFLPVRRLGEALDISVPFLAKI
                     LNQLVTAGLLKAHRGPTG"
BASE COUNT          831 a         1800 c         1814 g          781 t
ORIGIN      
        1 ccccaccaga tcgggccagc gcacgaacgg gttgcagttg ccctggatgg cctggatcac
       61 gctgtcccgc cgcatctcct cgcgcgatgg cgggtccgcg aggtgccacg cgagaaggtc
      121 aggcagctcg cgctcgaaga agagggcgtc gacccggtcg ggatacatga gtgcgaagta
      181 gaaatgggcg cgggcgaggt cgcccttcac ggggtcgggc ggctcgaagg cctccgctcc
      241 gatcttcgag acgtaatcgg cgtcgcccgg tggcgggccc cggaagccgt cgcctcccac
      301 cgcccaggtc tggaccaggg cgtcggacac ctcgccatac ggcaggttgc cccgccggct
      361 gttcgtgcgc tcgtccgacg gaaagagcat gtgcaggtcc gcctccgccg ggtacccgtc
      421 cgagccgcgc gagcgtggcc acgagtgctc cgtgttcacg ccctgccggt acagtacctg
      481 tgtcggatcc ccctcggtga gcggcacttc ccggccggtg tagaccgttc ggatcgtgcc
      541 cccacgcgcc tcggcccagt gggcgtagag ggtgtcgcga ccctcgttgt acgacaggac
      601 ccgccgggga gcgtaccggc cgcgcagcat tccgggcgag tcgccgagag gaggcgggca
      661 ggtctgcgcc agggcggcgg gcgccgcgag cgcggccacg aatgcggcaa gcgccagaat
      721 gccgtgtaat cgtgggtgca tcggaccgcc tccagcgtgg aaggtacggc ggaggcgacg
      781 gcctcccccg gcgtcgcacc gtgggaatcc gtgcgggaga atccccgctt gcccgcccgc
      841 cggggcaccg gtagactgcg cctcgatatc gccagtgcag ttcggcattc ggtggtctcc
      901 cttttgtaat aacggattca actcatggcc aggatgctcg ccgcggtctt ctgcctcgcc
      961 ctcgctgtcg ctccccttcc cctcgccgcc cagggcctca cacaggcgga atccgacgcc
     1021 atcgaggaca cggtcggccg gctgttcgcg gaaatcacgg aggcgacgaa cgccctggac
     1081 ctcgagcgcc ttctcgcgct ccacgaggac ggcgacgagc tgacctacgt ggccgccggc
     1141 cgagtgacga ggtcgcacga ggccttcgcc ggcctgctcc aggcgcagct cggcggcctc
     1201 tcggcggcgg acgtgcgcct gctggatccc tccgtcgacg tcctcgcgcg cgacgtcgcg
     1261 ctcgcgacct ccacgtacga attcacggcc accttcccgt ccgggagccg gttcacgacg
     1321 acgggcacgt acatggccgt cttcgtcctt cgggacggcg gatggaagat ccggtactcg
     1381 tcgcacacgt ttccgcagcg ggaaccgtag gatcggcacg gaccgatcgc ccctcggcgc
     1441 cggcccgtcc ggttcagcgt cccgggaacc aggccacgcc caggttcagg cgggtcgatg
     1501 tggcgtccag gttggtgttc cacgtgaggc cgttcaccct gacctcgctg atctcgccta
     1561 ccgtgaagag caggttcagg tcgagcgcga ggttcggcga cgtgaaatag agcacgcccc
     1621 cgccaagggt cagccctgcg ccgctggcct cgatgtcggc gcccggctgg tcgagctccg
     1681 cggcccggcc gttgagcgcg accatgaagt tgcccttcag cttggacgca tacgatccga
     1741 ccgtgaacat ggccccgagg tcgaagtgcc cgagcccgta gtcgttgccg gtgtcggggt
     1801 ctatgctggc gccggagaac tcgagatacg ccgccacgcg ctcattgaac ccgtagccca
     1861 gggccaggcc cagaccgcct ccggcctcgg cgtcttcctc gaagtcgtcc agccgccagg
     1921 ccgcgccgac gaggtgcggg cggacgaaga atccgcgcgt ggaatactga gccgacgccg
     1981 tgccgacgaa ggcgacggtc agggcggcga gaaggaggat gcgcttcatg tcatttctcg
     2041 gtggagtgac ggacaaatgc atctacaccc ggacgccgag cgtccgggtg aatgtatcgg
     2101 ccggaaaccc gggaaacatg agcggtaccg cccgattcgg cgcccaggga gatcccggcc
     2161 gccagcaccc cggtccggtt cgcgggattg ccgcgcgcac atattcctgt gaaggcatac
     2221 tgcacacgac cggcgttatg ctccgtccgg atggcggtgc gggcttcgat ccggcggacg
     2281 cggctgcatt cgacgaatcg cgaagccggg gcgggttcgg tcggccagca ttccagaacc
     2341 ggccgcccac gcggcccgcc cgccgtccgt tgtgtcgccg cggcacgctg ccgcgcgccg
     2401 aaccgccggc gtcttcagtg cgtcgcgagg tccatccgcc agttcgcccg gcgatacgcg
     2461 agctcgaacc ccgcgctcat caggttgcgc gtggacgggt tcggtttctc cggggtgtca
     2521 tcggccgtct cggtaacgag cagcgcgcat ccgtgccggg ccgcctcccg gatccggtgc
     2581 ttgatgaggg cggtctgggc tccacgccgg cggaactcct cgcgcgtgaa ggcggggccc
     2641 aggtaggccg agccgccgca cacgtacagg ccaccgtagg cggccggctc ggcgtcgatc
     2701 caggcgacgt acactttcca gcccggacgg tcgggcgttg caccgatggt ctcagccagc
     2761 ccgatcggcc aatcgaatgc ccgggtcaca tccagcccgt accgggggcc gagtcgcgac
     2821 ggcaggcggg tgatgtgggc gtccggctcc tcctccggcg gatcgtctac cgtcctgtag
     2881 agcttgagcc agtcgttgtg gtgctcgaag ccgcgctccg ccagcgcctc gggcaggcca
     2941 cgggcctccg cccggggcga gacgtgcacg aagaatcgcg gcgccctcgc ggtgcggaac
     3001 aggtcgacca cccggtcgat ggccgcctcg tcggcccgct cgtcccatag cccgatcgcc
     3061 cggttgtgga agaggacgtt gctgcggggc gcgcagaaga tcgtggcgtc ctccgtcagc
     3121 caggcggccc atccgaagcc gtcggtgcgg atggcgtctg cctcgcacac gtcgatccag
     3181 gcttcggcct cggcctgttc caggcggtcg atcagggttt cggacagcgc ggccatgtcc
     3241 ccatccggac gatcgttgac tccgggtacg cgcgagcggg cgattgggtg catggcacgg
     3301 atctcggcgc cgtgcacctc gcccgggagg gccctcggtg cgacgcgggg accgctcagc
     3361 tccccgccac cgcctccggg aagaacctgc ggcggaagaa gagcgacacc tgcacgagcc
     3421 cgatcagcac gggcacctcg accagggggc cgatgaccgt ggcgaacgcc tcggccgacc
     3481 cgatcccgaa gacggcgacc gccaccgcga tcgccagctc gaaatcgttg ctcgcggccg
     3541 tgagcgcgac ggtcgtcacc tcgggatacc gtccgcgcat cgcgcgcgcc gcgaagaagg
     3601 tgatgaggaa catgatgacg aagtacagga cgagcggcac cgccacccgg aggacgtcga
     3661 acggcagctc cacgatgagc tcgcccttca gcgagaacat gaccacgatc gtgaagagca
     3721 gggcgacgag cgtcacgggg ctgatcctgg ggatgaacgt cgtctcgtac cacgcgtcac
     3781 ccttcaggcg gagcagcacg agccgcgtca cgagtccggc ggcaaacggc actcccaggt
     3841 acagcagcac ggtcccggcg atctcgccga tcgagacgtc cacgatcgcg ccctccatcc
     3901 cgaagaacgg cgggagcaac gtgatgaaga accaggcgta gacgccgtag agcagcacct
     3961 ggaagacggc gttggcggcc acgaggccgg ccgcgagatc gcggtccccg tcggcgaggt
     4021 cgttccacac gaggaccatc gcgatgcagc gggcgagccc gacgaggatc aggccgaccg
     4081 cgtagccggg catgtccggg agaaacgcca tggcgagccc gaacatcacg agcggaccca
     4141 cgatccagtt ctgcacgagc gacagcgcca gcacccgccc gtgccgcagg accgcgccga
     4201 acttctcgta gcggaccttg gcgagaggcg ggtacatcat caggatgagc ccgacggcga
     4261 tcgggatgtt cgtcgtcccg acctggaagc ggtcgatgaa tccctcgacc ccgggcaaga
     4321 gcgccccgag cccgacgcca acggccatcg ccccgaagat ccagagggtg aggaaccggt
     4381 ccagcgtcga aagtcggcgg gtgacggcgt gcggcatggg tcgtgggaac ggggtggcgt
     4441 gcgcggccgg attccggaac gggggtaggc gcgttgcgcg cggccgcctt cgcggttcag
     4501 gcgggctccg gcaggtcggc gagggtatgg accagggcgc ggagatgctc gaaggcggcc
     4561 ggctccacgg catggcgtac ccacagaccc tccttgcggc tgcggattac gccggcctcc
     4621 cggaggacct tcaggtggtg ggtgattgtg ggctgcgtca ggtcgaaatc gcgctcgatc
     4681 tcgcagacgc aaaggtctcc ccctccggca tggatcagct ccgccatgcg caggcggacg
     4741 ggatggccga gcgccttcat catgccggcc gcgaactcga ggtcggcctc cggcaggagg
     4801 actcgcgctc gtccggggtc cggcgagcag gatgcgctcc gctgttcgtc cgtcattaca
     4861 tagatacccg tctatatact ggtcgacgcg aaaataggca ggtgttgcgg gcggcgcaat
     4921 ccccgcgcga gtcggatcgc cgacccggca atgccgcggg gtgccgcaag gtcgcgtatc
     4981 gccgtcgcgc cccgctcggt agcatttcct atattcgcct cgcttttccg gattgactgg
     5041 atgatttcac aggcatcgcg gtacgcgctg cgggcgctcg cctacctcgc aacccatgac
     5101 acggagcggt ttctgcccgt gcgtcggctg ggcgaggcgc tggacatctc ggttccgttc
     5161 ctggccaaga tcctgaacca gctcgtgacg gccggcctgc tgaaagccca ccgtgggccg
     5221 accgga
//
LOCUS       DBNHQZ010000420         3968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3241215, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000420 DBNHQZ010000000
VERSION     DBNHQZ010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3241215"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="AC16YU_11395"
     CDS             <1..556
                     /locus_tag="AC16YU_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065875.1"
                     /translation="TAEVRDCFPDARVPATACHCFPRINMKGARRPYGDRLVMIGDSG
                     VSRLYKDGIGAAYRTSKAAAMAAALHGVSADALRTHFLPACRAITFDNAIGKFIFGVA
                     HFIQKSRFLRRGVFRMTAFEQTTPGRARRMSSVLWDLFTGSAPYREVFLRTLHPVFLA
                     ILVWNLTVANLPGSTLPSGEPDVS"
     gene            553..948
                     /locus_tag="AC16YU_11400"
     CDS             553..948
                     /locus_tag="AC16YU_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6065876.1"
                     /translation="MKEVLGKTYAAGDDIVRQGEVGDCMFVIQEGEAEVLIDDDEGAQ
                     IRVDVMHAGEVFGEMAILERQTRSATVRALGPMRVLTIDRKTFLRRVQEDPSLAFMVL
                     KAMSNRVRKLDLELAALKAAASSAEGSTT"
     gene            945..2612
                     /locus_tag="AC16YU_11405"
     CDS             945..2612
                     /locus_tag="AC16YU_11405"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6065877.1"
                     /translation="MTPGNPSSGLDPGGFRFEPIRPVQDAGVPPCNGNCPSGTHIREW
                     IGLVAQRSKLGLTKEEAYRRAWESLVDRNPFPAVMGRVCPHPCESECNRTAKDGAVSV
                     NELERFIGDYAIERGWPLVRMTTATRSESVAVVGAGPAGLSFAYQMARRGYLVTVFDR
                     REQAGGMLRYGIPDYRLPPAVLDAEIARIMDLGVELRLGSAVGEHPTLDELRRGFDIL
                     FVGIGAQIGRRMGIPGEDGAGVWAGTEYLDRLNRGERVDTGRHVVVVGGGNTAIDAAR
                     SARRRDAAVVLLYRRTRAEMPAIGHEIDEAIEEGVEIVYLAAPSEILRDQTGTIRKVL
                     VQRMRLGEADASGRRRPVPIEGDTFDIAASAVIVAVSQEPDWTALDGLKRDGGWMRTA
                     ADGLLDANTYAGGDVRGLGTAAQAIGHGRAAAETAHARQDGTAYPGNGAKRHGTDLSR
                     VKLDLYEGAKRAAPERLPVPDRLADPLRTVTDTIDEEAFLAEASRCLSCESCFGCRHC
                     VTYCNGGGFVEVADPMPGMYFRMDLSVCEGCGKCIEVCPCGYLASES"
     gene            complement(2620..>3968)
                     /locus_tag="AC16YU_11410"
     CDS             complement(2620..>3968)
                     /locus_tag="AC16YU_11410"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6065878.1"
                     /translation="DDDEAERALSRLRDDVRDDAARQEVPTRAQTNGDARIHILVVDD
                     EPVNQQVLANHLAFSNYRISQAMNGRQALDLVQSDERFDLVLLDIMMPRMSGYEVCEQ
                     IRQRYLPSELPVIMVTAKNQVSDLVQGFDSGANDYIAKPFTKDELLARLKTHLNLLHI
                     NSAYGRFLPRDFLHYLGRESIIDVQLGDNVEMEMSIFVSDIRAFTTISEGMTPSENFQ
                     FVNDYMAVAGPVIREHSGFIDRYTGDSIMGLFAERPDDAVNAAIDTMRKVREMNRPRI
                     DAGLPEIRIGVGVHTGTLRLGIVGESERVQGDIFSDAVNLANRIEGLCKVYGVSIIVS
                     GETLGQLRDPSAYRSRFLGHAQVKGKTRAVSLYEIFDGDDEATIARREATRGDYAAGM
                     EAYFDRRFEEAAGRFAAVLEVDADDVTAAMYRDRCVLLTVSGVPDEWDGVQIIDRK"
BASE COUNT          630 a         1282 c         1392 g          664 t
ORIGIN      
        1 cacggccgag gttcgcgact gcttcccgga tgcccgggtg cccgccaccg catgccactg
       61 cttcccgcgc atcaacatga agggcgcccg gcgcccctat ggtgaccggc tcgtcatgat
      121 cggcgattcg ggggtttcgc gcctgtacaa ggacgggatc ggcgcagcct atcgtacgag
      181 caaggcggcg gcgatggccg ctgcgcttca cggcgtgtcg gccgatgctc tccgcaccca
      241 ttttcttcct gcctgccgcg cgatcacttt cgacaacgca atcgggaagt tcatcttcgg
      301 ggttgcccat ttcatccaga agagccggtt tctgcggcgg ggcgtatttc gcatgaccgc
      361 gttcgagcag acgacgcccg gccgcgcgcg acgcatgagc agcgtgctct gggacctgtt
      421 caccggctcc gcgccgtacc gggaggtgtt cctgcgtaca ctgcatccgg tcttcctggc
      481 catcctggtg tggaatctca cggtcgccaa cctgccgggc agcaccctgc cgtccgggga
      541 gccagacgtc tcatgaagga agtgctcgga aagacctacg cggcgggaga cgacatcgtc
      601 cggcagggcg aggtcggcga ctgcatgttc gtgatccagg agggtgaggc ggaggtcctc
      661 atcgacgacg acgagggcgc ccagatccgg gtcgacgtca tgcacgcggg cgaggtgttc
      721 ggcgaaatgg cgatcctgga acgacagacg cgctcggcta cggtgcgggc gctgggtccc
      781 atgcgcgtgc tcacgatcga ccggaagacg ttcctgcgcc gcgtgcagga ggacccttcc
      841 ctggcgttca tggtgctcaa ggccatgtcg aaccgcgtac gcaagctgga cctggagctg
      901 gccgcgctca aggccgcggc gtcgtccgcg gagggatcga cgacatgact ccgggaaacc
      961 cgtcatccgg gctcgatccg ggcggtttcc ggttcgagcc gatccgtccc gtgcaggacg
     1021 cgggcgtacc gccgtgcaac ggcaattgcc cgagcggcac gcacatccgg gagtggatcg
     1081 gcctcgtcgc gcagcgctcc aagctgggcc tgacgaagga ggaggcctac cggcgggcgt
     1141 gggagtctct cgtggaccgc aatccgttcc cggccgtcat gggccgggtg tgccctcatc
     1201 cctgcgagtc ggagtgcaac cgcacggcga aggatggcgc tgtttccgtt aacgagctcg
     1261 agcgattcat cggtgactac gccatcgagc ggggctggcc gctggtacga atgacgaccg
     1321 cgacccggag cgagagcgtg gccgtggtgg gcgcgggccc cgccggcctg tcgttcgcct
     1381 accagatggc ccgtcgcggc tatctcgtga ccgtgttcga ccgccgggag caggccggcg
     1441 gcatgctccg gtacggaatt cccgactacc gcctgccccc ggcagtgctc gacgcggaga
     1501 tcgcgcggat catggacctg ggcgtggagc tccggctggg ctctgcggtc ggagagcatc
     1561 cgacactcga cgagctccgg cgcgggttcg acatcctgtt cgtcggaatc ggagcccaga
     1621 tcggtcggcg gatggggatc cccggcgagg atggggcggg ggtatgggcc ggaacagaat
     1681 acctggatcg gctgaaccgg ggggaacgcg tggacacggg gcggcacgtc gtcgtcgtcg
     1741 gcgggggcaa cacggcgatc gatgcggcgc ggtcggcgcg gcgccgggac gcggcggtgg
     1801 tgcttctgta ccgtcggacg cgagccgaga tgccggcgat cggccacgag atcgacgagg
     1861 cgatcgagga gggagtcgag atcgtctacc tcgctgcgcc ttcggaaatc cttcgggacc
     1921 agaccggaac gatccggaag gtcctggtac agcgcatgcg gctgggggag gcggatgcga
     1981 gcggacgccg gaggccggtg ccgatcgagg gagacacctt cgacatcgcg gcctcggccg
     2041 tgatcgtcgc cgtatcgcag gagcccgact ggacggcgct ggacggcctg aagcgggacg
     2101 gcgggtggat gcgtacggca gcggacggac tgctggacgc gaacacctac gcgggtggcg
     2161 acgtgcgcgg cctgggaacg gcggcccagg ccatcggcca cggccgcgcc gccgcggaga
     2221 ccgcccatgc gcgccaggac ggcacggcct atcccggaaa cggcgcgaag cggcacggca
     2281 ccgatctttc gcgcgtgaaa ctggacctgt acgagggcgc gaagcgcgcg gcgccggagc
     2341 ggctgccggt gccggatcgc cttgcagacc cgctcaggac ggtgacggac acgatagacg
     2401 aagaggcatt cctcgccgag gcgtcgcgct gcctgtcctg cgaatcctgc ttcggctgcc
     2461 ggcactgcgt cacgtactgc aacgggggcg gattcgtcga ggtggccgat ccgatgcccg
     2521 gcatgtattt ccgcatggac ctctccgtct gcgagggatg cggcaagtgc atcgaggtct
     2581 gtccgtgcgg ttacctggcc tccgagtcgt gacgtacggt catttccggt cgatgatctg
     2641 gacgccgtcc cactcgtccg ggacaccgga caccgtgagc agcacgcagc ggtcccggta
     2701 catggcggcc gtcacgtcgt cggcgtcgac ttcgagtacg gccgcaaacc gcccggcggc
     2761 ttcctcgaac cgccggtcga aataggcctc catgcccgcg gcatagtccc cccgggtggc
     2821 ctcgcggcgc gcaatggtgg cctcgtcgtc cccgtcgaag atctcgtaga gcgagaccgc
     2881 tctcgtcttg cccttgacct gggcgtgccc caggaagcgg ctgcgatagg cggacgggtc
     2941 gcgcagctgg cccagcgtct ctccgctcac gatgatgctt accccgtaga ccttgcacag
     3001 gccctcgatc cggttggcca ggtttacggc gtccgagaag atgtcgccct ggacgcgctc
     3061 ggattcgccg acgatgccca gccggagggt gccggtatgc acacccacgc cgatgcggat
     3121 ctcgggcagc cccgcgtcga tgcggggccg gttcatttcc cgaaccttgc gcatcgtgtc
     3181 gatcgccgcg ttcaccgcgt cgtcgggccg ttccgcgaac aggcccatga tcgagtcgcc
     3241 cgtataccgg tcgatgaagc ccgagtgctc ccggatcacc gggccggcca cggccatgta
     3301 gtcgttgacg aactggaaat tctcggatgg cgtcatgccc tccgatatcg tcgtgaacgc
     3361 gcgaatgtcc gacacgaaga tggacatctc catctcgacg ttgtcgccca gctgcacgtc
     3421 gatgatggac tcgcgcccga ggtaatgcag gaaatcgcgc ggcaggaacc gaccgtaagc
     3481 cgagttgatg tgcagcaggt tcaggtgcgt cttgagacgg gccaggagct cgtccttggt
     3541 gaagggcttg gcgatgtagt cgttcgcgcc ggagtcgaag ccctgcacca ggtcggagac
     3601 ctggttcttg gccgtgacca tgattacggg caattcgctc ggcaggtatc gctgccggat
     3661 ctgctcacag acctcgtacc cggacatgcg cggcatcatg atgtcgagca gcacgaggtc
     3721 gaatcgctcg tccgactgta ccaggtccag tgcctggcga ccgttcatcg cctggctgat
     3781 gcggtagttc gagaaggcca ggtgattggc gagcacctgc tggttgacgg gctcatcgtc
     3841 gacgaccagg atatgaatcc gggcgtcgcc gttcgtctgg gcacgggtcg gcacttcctg
     3901 ccgtgccgcg tcgtcccgca catcgtcgcg caggcgcgaa agcgcccgct ccgcctcgtc
     3961 gtcatcca
//
LOCUS       DBNHQZ010000421         6028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2022641, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000421 DBNHQZ010000000
VERSION     DBNHQZ010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2022641"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC16YU_11415"
     CDS             <1..254
                     /locus_tag="AC16YU_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065879.1"
                     /translation="AQERTDLRMAQESLEAVRAEMTEMERALEEEIRELSARWDPETV
                     DLEEVHLTPRKSDVQVERLVLAWVPYASGPDGRRIPLHR"
     gene            complement(348..1745)
                     /locus_tag="AC16YU_11420"
     CDS             complement(348..1745)
                     /locus_tag="AC16YU_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6065880.1"
                     /translation="MRLSFFGAARTVTGSKHLLELDNGKRLLLDCGLFQGRREESDRL
                     NREFGFDPATIDAVLLSHAHIDHAGLLPKLWMEGFRGRIYATHATHSLSTLLLLDSAH
                     IQEKDVHFVNKIRRRRDKPSIEPLYRTAEAEGVLEHFVGVGYRQAFSPAPGVSVEYRD
                     AGHILGSASMVLTIEERGKTTRLGFTGDVGNPNRPILRDPQAMADCDYLISESTYGGE
                     VHDPPDRTRARLLEVITRTSARGGKVIIPSFAVGRTQEIVHALDRLWNEGKLPPIPVF
                     VDSPLAVSATGVFRAHPECFDREMNEYLLTDDDPFGFERLTYVRDVEESKALNGLNTP
                     AVIISASGMCEAGRILHHLRNNVESDRNTVMIVGYCADHTLGKRIVERRPEINIFGEP
                     HRLRAEVVVMNSLSAHADEPGMLDFIGKMDRERLRRIFLVHGSPDRQEKMRLALVSAG
                     YRGVEIPEHGESVEL"
     gene            complement(1742..2356)
                     /locus_tag="AC16YU_11425"
     CDS             complement(1742..2356)
                     /locus_tag="AC16YU_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065881.1"
                     /translation="MTRTPHRSAWRAATLLGLAVVVAWAPGCQTIREVAALRSVAFDF
                     GAVGDVRLAGIRFENVHSIDDLGLLDGARIVAAVADRNMPLSFVVGVLGTNPESNATT
                     ARLVRLEWTLFLEETETVSGVLDEPIALPPGETVNIPVAMQLDLFSFFDRSAADLVRL
                     AAGLTGVTDDATRIRIRATPTVDTPLGPIRYPEPISIVNVRTGG"
     gene            2425..3246
                     /locus_tag="AC16YU_11430"
     CDS             2425..3246
                     /locus_tag="AC16YU_11430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR6065882.1"
                     /translation="MKPGVLRTAVVQLRSTSDRKANLERAGAFVEEAAGRGARLVCLP
                     ENFGWLDREGRKIDVAETPEDGPSVALARDLAARLGIHVAAGSVALRTDDPGRTTNTQ
                     LVFGPDGSLLGSYDKIHLFDVDIDAEHSFRESRYVQPGDRVVTVDVDGWRVGLSICYD
                     LRFPELYRELAGRGAEVLLVPSAFTARTGRDHWETLLRARAIENLCYVAAAAQWGRHN
                     DRRESHGDAMIVDPWGEILARLPEGEGVLVADLEADRLEAVRRALPALDHRRLGV"
     gene            complement(3499..4827)
                     /locus_tag="AC16YU_11435"
     CDS             complement(3499..4827)
                     /locus_tag="AC16YU_11435"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6065883.1"
                     /translation="MLPGPASAQVLDDLADRLVAADPLPTAVAGKTATHGVLSSFLPD
                     DFTGVVVEARASSGAVTAEVRFLGGNGPGPWLPMRIVRSATSDALVAGYRSDLVRSGV
                     RVEVRIAAPDDAAVSIGQVGTFDNRLDQDRRPAPDGLAPLPAAATGTIAPPPLITRGE
                     WGAEPFIGTPSPLANPSYEYMTFHHAAGYSATTLAEGIEQVKAIQDLHQVVRGWSDIG
                     YQFLIDRGGRLYQGRPFMTDATSLEELPVLALGAHVGGANTGNIGVSLLGCYHPSEGS
                     YCEQVITPEAWDTYVTLFAFLSERYGVPTNRIRGHRDFSATACPGDNNYVLLPQLRTE
                     MLELLESGGGTLEDPDDFVLSNTFPNPADDEIRVRYALREPGFVSLDLFDATGRRVRK
                     LAAGYHETDTWFTASMDTADLPAGMYFYRIRVEGFAGVAYDRTRSVAVVH"
     gene            complement(4898..5956)
                     /locus_tag="AC16YU_11440"
     CDS             complement(4898..5956)
                     /locus_tag="AC16YU_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6065884.1"
                     /translation="MKHTTLRIVLALVLLTGMTTAARAQTFGESGEEPGTITKVGTTS
                     AQFLKLGVGARAIALGGAFVAEASDLSAIYWNPAGLATLPGGAVQFNHTKYLADIDYS
                     VAAFGTRLGRLGTLAASIIYLGSGDMAVRTTAQPDGTGERFSVSNLSIQLGFARSLTD
                     RFSIGTTFKYIREQIWHSSASALAFDVGVLFRTPFDRLRLGASMANFGPKMQMSGRDI
                     LFSTDPGANQSGNVEVVNAEYLMDRHALPLLFRVGVAMDAFTDANHRLLVLVDAAHPN
                     DNSEYVNFGAEYGFRDLVHLRVGHRNTFETDGEQGLTYGGGLGLRLDRSIRAQVNYAY
                     ADFGLLEATHWFSFDLSF"
BASE COUNT          964 a         1971 c         2174 g          919 t
ORIGIN      
        1 cggcccagga acgaaccgat ctgcgcatgg cacaggagag cctggaggcc gtgcgggccg
       61 aaatgaccga aatggagcgt gcactcgagg aggagatccg cgagctgtcc gcacggtggg
      121 atccggaaac ggtggacctg gaagaggtcc atctgactcc gaggaaatcg gacgtgcagg
      181 tggagcgcct ggtcctggcc tgggtgccct acgcgtccgg tcccgacgga cgcaggatcc
      241 cgctccaccg gtaggaggat ggggacggag tcccggcgac gacatcccgg aactcggcgc
      301 gggcctgctg aagggactcc tcgacggtcg ccgctgatcc ccccgggtca gagttcgacg
      361 ctttcgccgt gctccgggat ttccaccccg cggtacccgg ccgagacgag cgccaggcgc
      421 atcttctcct ggcgatcggg cgagccgtgc accaggaaga tccgtcgcag ccgctcccgg
      481 tccatcttgc cgatgaaatc gagcatgccc ggctcgtcgg cgtgggccga gaggctgttc
      541 atgaccacga cctcggcgcg caggcggtgg ggctccccga agatgttgat ctccgggcgg
      601 cgttccacga tgcgcttgcc gagcgtgtga tcggcgcagt agcccacgat catgaccgtg
      661 ttccggtcgc tctccacgtt gttgcgcagg tggtgcagga tccggcccgc ctcgcacatt
      721 ccggaggcag aaatgatgac ggcgggcgta ttcaggccat tgagcgcctt ggactcctcc
      781 acatcacgga cgtaggtcag tcgctcgaag ccgaacgggt cgtcgtcggt gagcaggtac
      841 tcgttcatct cgcggtcgaa gcactccggg tgggcccgga atacccccgt cgcgctgacg
      901 gcgagcggac tgtccacgaa aacggggatg ggcggcagct tgccctcgtt ccagagcctg
      961 tccagcgcat gcacgatctc ctgcgtgcgc cccacggcga aggacgggat gatcaccttg
     1021 cccccgcgtg cgctggtgcg ggtgatgacc tccaggagac gggccctcgt gcggtccggc
     1081 ggatcgtgca cctcgccgcc gtacgtggat tcggaaatga ggtagtcgca gtcggccatc
     1141 gcctgcggat cccgcaggat gggccggttg ggattcccga cgtccccggt gaagccgaga
     1201 cgcgtcgtct tcccgcgctc ttcgatcgtg agcaccatgc tggccgagcc gaggatgtgg
     1261 cccgcatccc ggtattcgac gctcacgccg ggagcgggcg agaaagcctg ccggtagccc
     1321 acgccgacga agtgctccag cacgccttcg gcctccgcgg tgcggtacag gggctcgatc
     1381 gagggcttgt cccgacggcg cctgatcttg ttgacgaagt gaacgtcctt ctcctggatg
     1441 tgggcgctgt cgagcagcag gagcgtgctc aggctgtggg tcgcgtgcgt ggcgtagatc
     1501 cggccgcgga agccctccat ccacagcttg ggcagcagcc cggcatggtc gatgtgcgcg
     1561 tgcgaaagga gcacggcgtc gatcgtcgcc gggtcgaacc cgaactcgcg gttcaggcgg
     1621 tcggactctt cacgccgccc ctggaaaagg ccgcagtcga gcagcaaccg cttgccgttg
     1681 tcgagctcga gcaggtgctt cgaaccggtg accgtacggg ctgccccgaa gaacgaaagt
     1741 ctcatcctcc ggtccgaacg ttcacgatgg agatgggctc ggggtagcgg atcggaccca
     1801 gcggggtgtc gaccgtgggt gtcgcacgga tgcgaatccg cgtggcatcg tccgtcacgc
     1861 cggtcaggcc cgcggcgagc cggaccaggt ccgcggcgct ccggtcgaag aagctgaaga
     1921 gatcgagctg catcgccacc ggaatgttga cggtctcacc gggagggagg gcgatgggct
     1981 cgtccagcac gccggacacg gtctccgtct cctcgaggaa gagcgtccat tcgagcctga
     2041 cgaggcgggc ggtggtggcg ttgctctcgg gattggtgcc cagcacgccc acgacgaagg
     2101 acaggggcat attccggtcc gccacggccg ccacgatccg ggcgccgtcg agcaacccca
     2161 ggtcgtcgat cgagtggacg ttctcgaacc ggatcccggc caggcggacg tcgccgacgg
     2221 cgccgaaatc gaaggcgacc gagcggaggg cagccacctc gcgaattgtc tggcatcccg
     2281 gagcccacgc gacgacgacg gccagcccga gcagcgtggc cgctcgccag gcagaccggt
     2341 gaggggtacg cgtcatgact atctttggaa tgattccgtg cgtcgccagt atacgggcgg
     2401 gccctgcgac ccgccaccgg aaccatgaag ccaggcgttc tccggaccgc ggtcgtccag
     2461 ctccgctcca cctcggaccg gaaggcgaat ctcgagcgcg ccggcgcctt cgtcgaggag
     2521 gccgccggcc gcggggcgcg gctcgtctgc ctgcccgaga actttggatg gctggaccgg
     2581 gagggccgca agatcgacgt cgccgagacg ccggaggacg gtccgtccgt cgctcttgcc
     2641 cgcgacctgg cggcccgcct gggaatccat gtcgccgcgg ggagcgtcgc cctgcggacc
     2701 gacgacccgg gccggaccac gaacacgcag ctcgtgttcg gtccggacgg gagcctgctc
     2761 gggagctacg acaagatcca cctcttcgac gtggacatcg acgcggagca ctcgttccgc
     2821 gagtcgcgct acgtgcagcc tggcgatcgc gtggtgaccg tggacgtcga cggctggcgg
     2881 gtgggcctgt cgatctgcta cgacctgcgc tttccggagc tctaccggga gctcgccggg
     2941 cgcggagcgg aggtgctgct cgtgccgtcc gccttcacgg ctcgcaccgg cagggaccac
     3001 tgggagacgc tcctccgggc ccgcgccatc gagaatctgt gctacgtggc ggccgccgcc
     3061 caatggggcc ggcacaacga tcggcgggag agccacgggg atgcgatgat cgtggatcct
     3121 tggggcgaga tcctggcccg gctgccggag ggcgagggcg tcctggtcgc ggacctggag
     3181 gcggatcgcc tggaggccgt ccgacgggcg cttccggcgc tcgaccaccg gcgtctcggc
     3241 gtctgaccgt accggacaaa cagaaagcag cccggcccac tgtgctaccc ccagggccgg
     3301 gctgcttcac ccagaaccaa tcatcactga gaggtgatcc accgagccgg ggctgtgccc
     3361 cgtggtggca ttgcaacgat acgacgaagt cataagacga atctgtaggt ggggtaccgt
     3421 ccgtcgcgtg ggcgaagtcc ctacacggct gccgggcatc ggaggatgcc ccgacactgc
     3481 gggaggggca gcggccgctc agtggacgac ggcgacggag cgcgtccggt cgtacgcgac
     3541 gccggcgaat ccctcgacgc ggatgcggta gaagtacatg ccggcgggga ggtcggccgt
     3601 gtccatcgac gcggtgaacc acgtgtccgt ttcatggtag ccggcggcca gtttccggac
     3661 gcgccggccg gtggcgtcga acaggtccag cgacacgaag cccggctcgc gcagggcgta
     3721 tcgcacccgg atctcgtcgt ccgccgggtt cggaaacgtg ttcgacagca cgaagtcgtc
     3781 cgggtcctcg agggtgccgc ctccggactc gagcagctcc agcatctccg tgcggagctg
     3841 gggcagcagg acgtagttgt tgtcgcccgg gcaggccgtc gccgagaaat cgcggtgccc
     3901 ccggatgcgg ttcgtcggaa cgccgtagcg ctccgagagg aaggcgaaga gcgtgacgta
     3961 cgtgtcccag gcctcggggg tgatgacctg ctcgcagtag gatccctcgg acggatggta
     4021 gcagccgagc agggacacgc cgatgttgcc ggtgttcgcg ccccccacgt gggcgccgag
     4081 ggcgaggacc ggcagttcct cgagcgacgt ggcgtccgtc atgaagggcc ggccctggta
     4141 caggcggccg ccgcggtcga tcaggaactg gtagccgatg tcgctccagc cgcggaccac
     4201 ctggtgcagg tcctggatgg ccttgacctg ctcgatccct tccgcaagcg tcgtggccga
     4261 gtagccggcc gcgtggtgga aggtcatgta ctcgtaggac gggttggcga gcggggaagg
     4321 cgttccgatg aacggctcgg cgccccattc cccgcgcgtg atgagcggcg gaggggcgat
     4381 cgttcccgtg gcggcggcgg ggagcggggc gagtccgtcc ggggcgggcc ggcggtcctg
     4441 gtccagtcgg ttgtcgaacg tgccgacctg gccgatcgac acggccgcgt cgtccggcgc
     4501 ggcgatgcgg acctcgacgc ggacgcccga gcggacgagg tcgctgcgat acccggcgac
     4561 cagggcgtcg ctcgtggccg accgcacgat gcgcatcggg agccacggcc ccgggccgtt
     4621 cccgcccagg aaccggacct cggccgtcac ggcaccggac gaggcccgcg cctcgacgac
     4681 cacgcccgtg aagtcgtccg gcaagaagga cgagagcacg ccgtgggtcg ccgtcttgcc
     4741 ggcgacggcg gtcggcaggg gatccgcggc caccaggcgg tccgcgaggt cgtccaggac
     4801 ctgcgccgag gcagggcccg gaagcaccag gaggaccgtg aatgcggcca ggagaacgac
     4861 ccgggacctc gcccgggccg ggtggcctgc cggcgactca gaacgagaga tcgaatgaga
     4921 accagtgcgt ggcctcgaga agcccgaaat cggcataggc gtagttcact tgcgcgcgga
     4981 tgctccggtc caggcgcagg ccgagacctc cgccgtaggt caggccctgc tcaccgtcgg
     5041 tctcgaacgt attgcggtgt ccgacgcgca ggtggaccag gtcgcggaat ccgtactccg
     5101 ctccgaagtt cacgtactcg ctgttgtcgt tggggtgggc cgcatccacg agaacgagga
     5161 gccggtggtt ggcgtccgtg aaggcgtcca tcgcgacgcc cacgcggaaa aggagaggga
     5221 gggcgtggcg gtccatgagg tactcggcgt tcacgacctc gacgtttccg gactggttcg
     5281 ctcccgggtc cgtgctgaag aggatgtccc gaccgctcat ctgcatcttg ggaccgaaat
     5341 tcgccatgct cgcgccgagc cgcaaccggt cgaacggggt ccggaacagt acgcccacgt
     5401 cgaacgccag cgccgacgcc gaggagtgcc agatctgctc acggatgtac ttgaaggtcg
     5461 tcccgatcga gaatcggtcc gtgagcgagc gggcgaagcc gagctggatc gagagattcg
     5521 acacgctgaa ccgctcgccc gtgccgtcgg gctgcgccgt ggtgcggacg gccatgtcgc
     5581 ccgatcccag gtagatgatc gaggcggcga gcgtgcccag ccgtccgaga cgggttccga
     5641 acgcggccac gctgtagtcg atgtccgcca ggtacttcgt gtggttgaac tggacggcgc
     5701 cgcccggaag cgtggccagg ccggccgggt tccagtagat ggccgacagg tcgctcgcct
     5761 cggcgacgaa cgcgcccccc agggctatgg cccgggcccc gacgcccagc ttcaggaact
     5821 gcgcggaggt cgtgcccacc ttggtgatcg tgccgggctc ttccccggac tcgccgaagg
     5881 tctgggcacg ggccgccgtg gtcatgcccg tcaggaggac cagcgcgagg acgatgcgta
     5941 gagtcgtgtg cttcatttcg ctgggtatcc gggctacttg atgaccgcga acttgccgat
     6001 gtacgatcca cccggcccgc cccgcgcg
//
LOCUS       DBNHQZ010000422         4423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1077229, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000422 DBNHQZ010000000
VERSION     DBNHQZ010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1077229"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..677)
                     /locus_tag="AC16YU_11445"
     CDS             complement(402..677)
                     /locus_tag="AC16YU_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HIR6065885.1"
                     /translation="MIKKRRITVDEARQAGADLGLDWSKVDLEQFRHGLEIELEHGAR
                     DPETDVTGDDLALTGRIAWAHLKEFPDYYTRLDKLEAEADAYWASRR"
     gene            complement(913..1812)
                     /locus_tag="AC16YU_11450"
     CDS             complement(913..1812)
                     /locus_tag="AC16YU_11450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR6065886.1"
                     /translation="MRILGRSQLAFSLALGLILGLGAGAISAFGTGTGWLPGLVVLSV
                     FLFFALRKSVGRWRATRRPLPPGATQWLERSVGLYRRLGREGRRRFETDVQIILDEWI
                     FEGVGQMEVTFEMRLAVAAGVALLLHGRPDWELPHRQTVLFYPDSFDDEYLVESEGEF
                     DGMAHQQGPIILSAKALEESWRDGEDGGNVVLHELAHLLDYKTEFADGVPSLVDPRSI
                     AAWQALVRKEMRRIRRRRSILDGYGATDQAEFFAVAVESFFEQPDLMERRHPELFTAL
                     KAAFNMDPRTGSALLPENRFADE"
     gene            complement(1874..2380)
                     /locus_tag="AC16YU_11455"
     CDS             complement(1874..2380)
                     /locus_tag="AC16YU_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6065887.1"
                     /translation="MSTVKRIRFTARIEAHPALVYERMTGDESYRDWTSAFIKGSRYE
                     GSWARGQKIRFLSPAGDGMVAEIAENRPGEFVSIRHLGFIAKGVEDTSSEAVRAWAPA
                     FENYTFQAVDGGTRLVVDQDVTAEHEDYMREAWPRALERLKTLCEGGDGEWKLLANMW
                     TTRPRENG"
     gene            complement(2686..>4423)
                     /locus_tag="AC16YU_11460"
     CDS             complement(2686..>4423)
                     /locus_tag="AC16YU_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6065888.1"
                     /translation="GVVLMLFVIGLDLEPAKLWRLRKPIQGLGGQQVGVSGIVLAGAG
                     LAFGLGIRPALAVGMTLALSSTAIVLQTLAEKGQLDSEGGRSTFSVLLFQDIAVIPML
                     ALMPLLATAGASAPGVGHGEAAAWLAGLPPLAQTLFVLGAVGAVLAGGRLLVRPVLRI
                     VAAARLREVFTAAALLLVIGVALLMQGVGLSPALGTFLAGVVLANSEYRHELESDIEP
                     FKGLLLGLFFIAVGASIDFALIGSRPGLILGITAGILLTKGAILLGLTRMFRLGLDSA
                     LLVAFALPQVGEFAFVLLSFARQEGVLEAGITDPLVAAVALSMAVTPLLLLLNDRVIR
                     PRFGTRAEASRPADAIDHRAEVLIAGFGGVGSTIGRFLRANGVATTVLDSDPERVDLL
                     RRIGLRVYYGDATRHDLLETAGAADARILVVALGTPETTLQVVRMARRHFPHLAVVAR
                     AFDWADAHDLVEEGVEHVVRQSLEPGLRLGTEVLRRKGFRAYQAHRAALRFLHHDMES
                     QQYLTGKRREDRKDYLSAVRARIEDLEQILLRDLREADQPRDEGWDDTTLREEFGGLP
                     PRGDVDPDGADR"
BASE COUNT          734 a         1524 c         1502 g          663 t
ORIGIN      
        1 gaacccgcca ccaaagaaca agctgaacag aatgaggacg gacgcgaccg tggatgacgg
       61 ggcctcattc atggcgtcgc ctggatgcgt gtaacggccg gcgggtatac ggcgggcaca
      121 ttctcactcg cccaggtcca gaatacccaa gcgatcccgg aaccgccatc cagcgccact
      181 tcaccgcgga atcctgatcg tcgtcggtgt ccgctctgca atgtcggtca tcatcttgtc
      241 gtccgcaggc ccgttacacg ctgatcgccg ggaggcacag gccgagagag ggattgcttt
      301 cattccggta tcgggatgaa accctcacag atgccggtcg gccgacaccc ctggacgagt
      361 gccgcgcccc ggcagcttca tgcaccggtc aggtggcgtg ctcaacggcg cgatgcccag
      421 taggcatcgg cttcagcctc gagtttgtcc agacgcgtgt agtagtcggg gaactccttc
      481 agatgcgccc acgcgatcct ccccgtaaga gccaggtcat cgcccgtgac gtcggtttcc
      541 gggtcccggg caccatgctc gagctcgatt tcgagtccat gacggaactg ttcgaggtca
      601 accttggacc agtcgaggcc gaggtccgcg ccggcctgtc tcgcttcgtc aactgtgatg
      661 cggcgcttct tgatcatgat gtcttctcgc tgttgcatgc cgggcaggtc agttgttcgt
      721 ggattgcgaa cggtgcgcgc cggctgacga atggccgctc tcccgtgaaa ggcagcgcgg
      781 cgtatggaac agtcagtgta gatgcggagg ctccctcgag gcaagaaggc agattccctc
      841 gtcgggtgca gtggcaggct ttacagccgt ggcacctgtc cactgaagcg ggtcagccgc
      901 gatcgaacct ctctactcgt cggcgaaccg gttttcgggg aggagtgcag aacctgtccg
      961 agggtccatg ttgaatgccg ccttcagcgc ggtgaaaagc tcagggtgcc gcctttccat
     1021 cagatcgggc tgttcaaaaa agctctccac cgctacggcg aagaattcgg cctggtccgt
     1081 cgcaccgtaa ccatccagga tggagcgcct ccgccgtata cgtctcattt ccttccgtac
     1141 gagtgcctgc caggccgcga tggagcgggg gtcgacgagg gaaggcacgc catcggcgaa
     1201 ctccgtcttg tagtcgagca ggtgggccag ttcgtggagt acgacgttcc cgccatcctc
     1261 tccgtcccgc cagctctctt cgagcgcttt cgcggagagg atgatgggac cctgctggtg
     1321 cgccatcccg tcaaactcgc cctcgctctc gacgagatac tcgtcgtcga aggaatccgg
     1381 gtagaagagg acggtctgcc ggtgcgggag ctcccaatcg gggcggccgt gcagcagcag
     1441 cgcgacgccc gcggcgacgg cgagtcgcat ctcgaaggtg acctccatct gcccgacacc
     1501 ctcgaagatc cactcgtcga ggatgatctg gacgtccgtc tcgaaccggc gccgcccctc
     1561 ccggccgaga cgacggtaca gcccgacgga acgctccagc cattgcgtcg cgccgggagg
     1621 aaggggcctg cgggtcgcgc gccatcgccc gaccgatttc cgcagggcga agaagaggaa
     1681 caccgacagg acgacgagcc cgggcagcca ccccgtaccc gttccgaacg ccgaaatggc
     1741 gccggcgccc aggcccagga tcaggcccag cgcgaggctg aaggcgagtt gggatcggcc
     1801 gaggatgcgc atgcgcggtg acacgtcgac cgggcaggag ggttcgcccg ttcgccttcg
     1861 cctcgtggat cggctacccg ttctcccggg gacgcgtcgt ccacatgttc gcgaggagtt
     1921 tccattcgcc gtccccgccc tcgcagagcg tcttgagcct ctcgagcgcc ctgggccatg
     1981 cctctctcat gtagtcctcg tgctcggccg ttacgtcctg gtcgacgacg aggcgcgtcc
     2041 cgccgtccac cgcctggaag gtgtagttct cgaaagccgg ggcccacgcc cgcacggcct
     2101 cgctcgaggt gtcctcgacc cccttcgcga tgaagccgag atgccggatc gagacgaact
     2161 cgcccgggcg gttctcggcg atctcggcga ccatgccgtc tccggccggg gagaggaagc
     2221 ggatcttctg cccccgggcc caggacccct cgtagcgcga tcccttgatg aaggccgaag
     2281 tccagtccct gtacgactcg tcgccggtca tgcgctcgta cacgagggcc gggtgcgcct
     2341 cgatgcgggc cgtgaaccgg atgcgcttga cggtggacac gcgaggattc gatggaggcg
     2401 gggtacggcg cggcggccag gtgccgggcg tggcgggcca tggcccatga tcggtgcggc
     2461 gcggcgagcg cgcaaccggc gcttcggccg gcgcaactgc tgccagagga cgcgagccgt
     2521 ccgggcacga taccgagccc gatgcagaag cgccagtccg gatccgcgcc gtgcccgagc
     2581 gtggccgcgg ggcggaatgc ggcggacacc gcgaagggga agcattccgg cgctcccggt
     2641 tccgggtacc cgcgccgggc gcccggccgt gccctgaaca cggggtcagc gatccgcgcc
     2701 gtccgggtcg acgtcgccgc gcggcgggag gcctccgaac tcttcccgga gcgtcgtgtc
     2761 gtcccatcct tcgtcccggg gctgatccgc ctcgcgaagg tcacggagca ggatctgctc
     2821 gaggtcctcg atccgcgcac gcacggcgga caggtagtcc ttccggtcct cccggcgctt
     2881 ccccgtgaga tactgctgcg actccatgtc gtgatgcagg aaccgcagcg ccgcgcgatg
     2941 cgcctggtag gcccggaatc ccttgcgccg caggacttcc gtgccgagcc ggagacccgg
     3001 ctcgaggctc tgccgcacga cgtgctccac cccctcctcg acgaggtcgt gcgcgtccgc
     3061 ccagtcgaag gcccgcgcca cgacggcgag atgcgggaaa tgcctcctgg ccatccgcac
     3121 gacctggagc gtggtctccg gcgtgccgag cgccaccacc aggatccggg cgtcggcggc
     3181 cccggccgtt tccaggaggt catggcgcgt cgcgtcgccg tagtacacgc gcagcccgat
     3241 ccggcgcaga aggtccaccc gctccggatc ggaatcgagc accgtcgtgg cgaccccgtt
     3301 ggcgcgcagg aaccggccga tcgtggagcc cacgccgccg aagcccgcga tcagcacctc
     3361 ggcgcggtgg tcgatggcgt cggccggccg tgacgcctcg gcgcgcgtcc cgaaccgcgg
     3421 gcggatcacc cggtcgttga gcagcaggag cagcggggtg acggccatcg agagggccac
     3481 ggccgccacg agcggatccg tgattccggc ctcgaggacc ccctcctggc gtgcgaacga
     3541 cagcagcacg aaggcgaact cgcccacctg cggcagcgcg aaggccacga gaagggcgct
     3601 gtcgaggccg agccggaaca tccgggtcag gccgaggagg atcgccccct tcgtcaggag
     3661 gatcccggcc gtgatgccga ggatcaggcc cggccgagag ccgatcagcg cgaaatcgat
     3721 ggacgcgccg accgcaatga agaacagccc cagcaggagg cccttgaagg gctcgatgtc
     3781 gctctcgagc tcgtggcggt actcgctgtt ggccagcacc acgcccgcca ggaaggtgcc
     3841 gagcgcgggg ctcaggccca cgccctgcat gagcagcgcg acgccgatca cgagaagcag
     3901 ggccgccgcg gtgaacacct cgcgcaggcg agcggccgcc acgatccgga gcaccggacg
     3961 cacgagcagg cgtccgccgg caagaacggc gcccaccgct cccagcacga acagcgtctg
     4021 cgccaggggc ggaagcccgg cgagccaggc cgcggcctcg ccgtgtccga cgcctggggc
     4081 cgatgcaccg gcggtagcca gcagcggcat gagggcgagc atggggatga cggcgatgtc
     4141 ctggaagagc aggacgctga acgtgcttcg gccgccctcc gaatccagtt ggcccttctc
     4201 cgccagcgtc tggagcacga tggctgtgga ggagagggcg agcgtcatgc cgaccgcgag
     4261 cgccggcctg attccgagcc cgaacgccag gcccgcaccg gcgagcacga tcccgctcac
     4321 gcccacctgc tggccgccca gcccctggat gggcttgcgc agtcgccaga gcttggcggg
     4381 ctcgagatcg agcccgatca cgaacagcat gaggaccacg ccg
//
LOCUS       DBNHQZ010000423         2440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3241512, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000423 DBNHQZ010000000
VERSION     DBNHQZ010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3241512"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="AC16YU_11465"
     CDS             <1..1709
                     /locus_tag="AC16YU_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6065889.1"
                     /translation="PVEGVTRLALALPANQALVASSGRRVAFAPDGQSFVYTGPGSGG
                     SQLWLRALDTLDATAIPGTAAATSPFFSPDGHEIGFLTLAPFALKVVPLAGGEPRIVT
                     SEVSGGAAVWGTDGYIYFDAGDNLSRIHPDGTGREVVSRLDTLQRETGFAWPTMLPDD
                     RGLLIRLRHAGEAPDEYHIVAIAPRTGKRSAVVRAVLGQYAPSGHLLYVTADGSLFAQ
                     TLDLNELALTGSPHLLWRGMSIGGFGSADLVLSPSGTLLYTSGVGTTAITELAWVTRG
                     GAITSTDTTRPDGLLRDLAVSPDGSRIALQIAATSTGASNKEDIWVKQLGGGPTSRLT
                     IEGMVNRHPTWSPDGREVFFLSNRDGGFAVYRQRWDGSRPAERVTTEPRGIGAVTVSS
                     DGRALVVQSEAGASGYGDLLIYRLGLDTAPEPLVATPAREYAPTLSPDGRWLAYTSDE
                     SGLTQVYVRPFPDVGRFKKQVSTAGGAGPRWSPIGDALYYRSATDDLMAVAVRATPDF
                     SAAPPERLFSATPYATAYDVSPDGQRFLMLHFATAAGGQEPDRLVIVQHFLADLERRL
                     PR"
     gene            1755..1919
                     /locus_tag="AC16YU_11470"
     CDS             1755..1919
                     /locus_tag="AC16YU_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065890.1"
                     /translation="MCDKLSGMATGYLAHNVKDDRLTAARLGATNQRGEHGTILVNTF
                     FDELTAVVGR"
     gene            1916..2128
                     /locus_tag="AC16YU_11475"
     CDS             1916..2128
                     /locus_tag="AC16YU_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065891.1"
                     /translation="MTEPTARLSTAVADRYRIERELDAGGMATVYLAYDVKHDRKVAI
                     KVLRPELAAVLGAERFVQEIMTTGNL"
BASE COUNT          400 a          827 c          802 g          411 t
ORIGIN      
        1 cgcctgtcga gggtgtgact cgactcgcgc tggcgctccc tgccaaccag gcgctcgtcg
       61 cgtccagtgg acgacgcgta gcattcgcac cagacggaca gtccttcgta tacacgggac
      121 cgggcagtgg cggcagtcaa ctctggctgc gggccctcga tacactcgac gccacggcca
      181 ttcccggcac cgccgcggcc accagcccgt tcttctcccc ggacgggcac gagatcgggt
      241 tcctgactct cgcgccgttc gcgctcaagg tcgttccgct cgctggtggc gagccgcgca
      301 tcgtgaccag tgaagtctcg ggtggcgccg cggtctgggg gaccgatggg tacatctact
      361 tcgatgcggg agacaacctc tcgcgaattc atcccgacgg gacgggccgc gaagtggtct
      421 cgcgactcga tacgctccag cgcgaaacgg gcttcgcctg gcccaccatg ctgcccgacg
      481 accgggggct gctcatacgc ctccgccacg ctggcgaggc tccggacgag tatcacatcg
      541 tcgcgatcgc cccccggacg ggcaagcgga gcgccgtggt gcgcgccgta ttggggcaat
      601 acgctccttc gggacatctg ctctacgtca cggcggatgg atcactgttt gcgcagacct
      661 tggacctcaa cgagctcgcc ctcacgggct caccgcatct gctgtggcgt gggatgagca
      721 tcggtgggtt tggatcggcc gatctcgtgc tgtctccttc cggaacgctg ctctacacga
      781 gcggcgtggg caccacggcc atcaccgagt tggcctgggt cacgcgcggc ggggcgatca
      841 cgtcgaccga caccactcgg ccggacggcc tgctgcgcga cctggccgtg tcaccggacg
      901 gatccaggat cgcactgcag atcgcggcca cctccacggg cgcgtccaac aaggaggaca
      961 tctgggtgaa acaacttggc ggcgggccaa cctctcggct caccatcgag ggcatggtca
     1021 accggcaccc gacgtggtcg cccgacggtc gcgaagtctt cttcctttcg aaccgggacg
     1081 gtggattcgc ggtgtaccgg cagcgatggg atggcagccg ccccgcggaa cgcgtgacga
     1141 ccgaaccgcg agggatcggg gccgtgaccg tctcttcgga cggccgcgcg ctggtggtgc
     1201 agagcgaggc cggggcaagc ggttacgggg acctcctcat ctaccgcctg gggctcgata
     1261 ccgcccccga accgttggtg gccacgcccg cccgggaata cgcgcccacg ctctcgccgg
     1321 acggccgctg gctcgcctat acatcggatg agagcggcct tacgcaagtc tatgtccgcc
     1381 ccttcccgga cgtcggccga ttcaagaagc aagtctcgac cgctggaggg gcagggcccc
     1441 gctggagtcc aataggcgac gcgctgtact atcggagcgc cacggacgac ctcatggcgg
     1501 tggcggtacg ggccacgccc gatttctcgg cggccccccc ggaacggctc ttttccgcca
     1561 ccccgtatgc gacagcctac gacgtgtcgc ccgacggaca gcgcttcctc atgttacact
     1621 tcgcaacggc ggccggtgga caggagcccg accggctggt catcgtgcag cactttctcg
     1681 cggacttgga gcggcggttg ccgcggtagc ggggcgttcc tctattgcgg gatgtcctac
     1741 atccagggcg agacgtgtgc gacaagctca gcgggatggc caccggctac ctcgcccaca
     1801 acgtcaagga tgaccgcctc accgccgcgc gcctgggtgc cacgaatcaa cgaggcgagc
     1861 acggaacgat cctcgtgaac accttcttcg atgaattgac ggccgtggtg gggcgatgac
     1921 cgaaccgact gcacgccttt ccacggccgt cgccgatcgc taccggatcg agcgcgaact
     1981 cgacgccggt ggcatggcca cggtgtacct ggcctacgat gtcaaacacg accgcaaggt
     2041 ggccatcaaa gtcctccgtc ccgagctggc cgccgtgctc ggcgccgagc ggttcgtgca
     2101 ggagatcatg accacgggga acctgtagca tccgaacatc ctgcccctct tcgattctgg
     2161 agaggcagcg ggtcccgttt gggcccacgg tttcttggcg gtctgagtga gggtcaccag
     2221 ggatctcggt aaccctatcg ataaccctac cggccgtcat gcccagacgc agggagcgcg
     2281 gttgagcggt gtttggtggc taggcgggca tggcggaacc ggaggccagg cagcacgtta
     2341 gagcgagcga gcgctgtcgt gtcatgtcga gtgcgcgcac ctgacgccgc cctcataatc
     2401 cgtcggtccc aggttcgaat cctggagggc ccattcacgg
//
LOCUS       DBNHQZ010000424         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1752733, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000424 DBNHQZ010000000
VERSION     DBNHQZ010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1752733"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16YU_11480"
     CDS             complement(<1..254)
                     /locus_tag="AC16YU_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6065892.1"
                     /translation="MDRLWSPWRSVHVTGWDGKSPEEKDRLLERIGESSDDEANLVVW
                     RGGAVFVLMNRYPYNNGHMMIAPYRAVASYEARREDEQIE"
     gene            330..1163
                     /locus_tag="AC16YU_11485"
     CDS             330..1163
                     /locus_tag="AC16YU_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6065893.1"
                     /translation="MKTDFHLHSTASDGTLDADALVRELAAREVAWAGLTDHDTVAGW
                     SSASREAERLGIEITPGAELSVVCHGDPVHLLALGFDPEHPPLAAWLDRFRTDRRERA
                     EAMLRKLRGAGIPVDASVLPAEAGDSVVTRPHIADAVVAAGGAATNGEVFERYIGDGA
                     PCFVPMPPRASAEAIRVIHGAGGIAVLAHPGDWTAHPTVLELVREGLDGIEAGHPTHD
                     DSLRAYYEAQAIGHGLVVTAGSDFHGRSAAEFDRLGGYALTEPLRDLLRERLRKARTR
                     A"
     gene            1209..>3442
                     /gene="porZ"
                     /locus_tag="AC16YU_11490"
     CDS             1209..>3442
                     /gene="porZ"
                     /locus_tag="AC16YU_11490"
                     /inference="COORDINATES: protein motif:HMM:NF052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system anionic LPS delivery
                     protein PorZ"
                     /protein_id="HIR6065894.1"
                     /translation="MAHRTRRRATACLRIAAVLAAALGLTRTAVARQVAGPDLWEAHT
                     SMQFVREVAAGGDRIWAATSGGIFGYGLQGGEIERFTVVEGLHSVDTGTLAWDARRSV
                     IWAGYFDGVLDRIDPESGAVTQFRDIERAGQFSSRGINRIRVVGDSLLVATEFGVVVF
                     DAVRGEVRDAYTRMGSFTAATPAFDLLIADLDGVPTLWVATAEGLAHAPLDAPNLQDP
                     ASWSNETSGLPPGAEAVWSVGMHRDRIYVGTETDLFERTPTGFEPLLVTTRGVGRIVT
                     VGGQLYAAERFLLAYVDAEGASGTLQVPGYENPVGLATDLDGGLWIGDNLGGLLSVTH
                     APGSGAVVVNLAIVPEGPADTQFTDMEFADDGTLWAASFTNTFSGFHRRSVDGIWTAY
                     TRLNRPELELRSSYMLIHAAPDGSGWAGSEGNGLARVLPDESIETYDHTNSSLRPATG
                     TAAYVIVGGMDSDAAGRLWVTSRGAGNPLHVRATDGSWTGLPPFVGEGLTSRATAYGR
                     IMVDSYGQKWVIVHDENNFRVVKGLLVADTGSDPASPEDDSFRAFLQKGAAGQGLPSL
                     TVTSVAEDRDGLVWIGTESGIAYVLNTGIVARDASARPIWPPWADRSQGTFVLFGLHV
                     NDLAVDPANRLWVASNEGAWLIEQVEAGYALVEHFTEENSPLFSNEVLSVAIEERTGE
                     VFFATDRGTVSYAGDAIAPVAEADDLLVYPNPFRPGAGTGTVTFEGLVEETDIRVVTP
                     GGIVV"
BASE COUNT          493 a         1173 c         1266 g          510 t
ORIGIN      
        1 atctcgatct gctcgtcctc ccggcgcgcc tcgtacgagg cgaccgcccg gtagggtgcg
       61 atcatcatgt gcccgttgtt gtacggatag cgattcatca gcacgaacac ggccccgccg
      121 cgccagacca ccaggttcgc ctcgtcgtcc gacgactcgc cgatgcgctc gagcagccgg
      181 tccttctcct ccggcgactt tccgtcccat ccggtgacgt gaaccgagcg ccacgggctc
      241 cagagccgat ccatgttcga gggggcaaat cctgtgggac gggagtatac gccgccgacg
      301 agaaacgcag tacggatcgc ccatcgccca tgaagaccga ctttcacctg cactcgaccg
      361 cgtccgacgg gaccctggac gcggacgcgc tggtccggga gctggccgcc cgggaggtcg
      421 cctgggcggg gctcaccgac cacgacacgg tggcgggttg gagttccgct tcccgcgagg
      481 ccgagcgcct cggaatcgag atcaccccgg gggcagagct ttcggtcgtg tgccatggcg
      541 atccggtcca cctgctggcg ctcggattcg atccggagca tccgccgctc gccgcgtggc
      601 tcgaccggtt ccgcacggac cggcgggagc gcgcggaggc catgctcagg aagctgcgcg
      661 gcgccggcat tccggtggac gcgtccgttc tccccgccga ggcgggcgac agcgtggtca
      721 cgaggcccca catcgcggac gccgtggtgg ccgcgggcgg cgccgcgacg aacggagagg
      781 ttttcgagcg gtacatcggg gacggagcgc cgtgcttcgt ccccatgccg ccgcgggcct
      841 ccgccgaggc catccgcgtc attcacgggg cgggcggcat cgccgtgctc gcgcacccgg
      901 gcgactggac cgcgcacccg accgtgctcg agctggtccg ggagggcctg gacggcatcg
      961 aggcgggtca tcccacgcac gacgactcac tcagggccta ctacgaggcc caggcgatcg
     1021 gacacggcct ggtcgtgacc gcgggctcgg atttccacgg ccgctcggcg gcggaattcg
     1081 accggctcgg cggctacgcc ctgacggaac ctctccgcga cctcctgcgt gagcggctcc
     1141 gcaaggcccg gacgagggcc tgacggagcc ctgtcgcacg ggacccgacc ggtccgcaac
     1201 cctgatccat ggcacatcgc accagacgcc gggccacggc ctgtctccgg atcgcggcgg
     1261 ttctcgccgc ggccctcggg ctcacccgga cggcggtcgc ccggcaggtc gccgggccgg
     1321 acctgtggga ggcgcacacc tccatgcagt tcgtccgcga agtggccgcg ggcggggacc
     1381 ggatctgggc ggcgacgtcg ggggggatct tcggatacgg cctgcaggga ggcgaaatcg
     1441 agcggttcac ggtcgtcgag ggccttcaca gcgtggatac ggggacgctg gcctgggatg
     1501 cccgtcgctc cgtgatctgg gcgggctact tcgacggcgt gctcgaccgg atcgacccgg
     1561 agtcgggagc cgtgacgcag ttcagggata tcgagcgggc cggccagttc tcctcgaggg
     1621 gcatcaatcg catccgggtg gtcggcgatt cgctgctcgt cgccacggag ttcggggtcg
     1681 tcgtcttcga cgccgttcgt ggcgaggtcc gggacgcgta cacccgcatg ggctcgttca
     1741 ccgcggcgac gcccgcgttc gacctcctga tcgcggacct ggacggggtg ccgacgctct
     1801 gggtggcgac ggccgaaggc ctggcgcacg ccccgctcga cgccccgaac ctgcaggatc
     1861 ccgcctcgtg gtcgaacgag acctcgggcc tgccgccggg agcggaggcc gtatggagcg
     1921 tcgggatgca tcgcgaccgc atctacgtgg gtacggaaac cgacctgttc gagaggactc
     1981 cgaccgggtt cgagccgctc ctcgtcacga cgcgcggcgt gggccggatc gtgacggtcg
     2041 gcggacagct gtacgcggcg gagcgtttcc tgctcgccta cgtggacgcg gagggcgcgt
     2101 cggggaccct gcaggtcccc gggtacgaga acccggtggg gctcgccacg gacctggacg
     2161 gcgggctctg gatcggggac aacctcggcg ggctgctctc ggtcacgcac gcgcccggca
     2221 gcggcgcggt cgtggtgaac ctggcgatcg ttcccgaggg gccggcggac acgcagttca
     2281 ccgacatgga gttcgcggac gacggcacgc tctgggccgc gtcgttcacc aacacgttct
     2341 cgggcttcca tcggcgaagc gtggacggga tctggacggc ctacacgcgc ctgaaccggc
     2401 ccgagctcga gctccggagc agctacatgc tgatccacgc ggccccggac ggctcgggat
     2461 gggccggatc ggaggggaac ggcctggccc gcgtgctgcc ggacgagtcc atcgagacct
     2521 acgaccacac gaattcctcg ctccgccccg cgaccggcac ggcggcctac gtgatcgtgg
     2581 gagggatgga ctcggatgcc gcaggaaggc tctgggtgac gtcgcgcggc gccgggaatc
     2641 cgctgcacgt gcgggcgacc gacgggtcgt ggaccggcct gccgcccttc gtgggcgagg
     2701 ggctgacgag ccgcgcgacc gcctacggcc ggatcatggt cgactcgtac ggccagaagt
     2761 gggtcatcgt ccacgacgag aacaacttcc gcgtcgtgaa ggggctcctg gtggcggaca
     2821 ccgggtcgga cccggcgagt ccggaggacg attcgttccg ggcattcctc cagaagggcg
     2881 cggccgggca ggggctgcct tccctcacgg tgacgtcggt ggccgaggac cgggacgggc
     2941 tggtctggat cggcacggag agcggcatcg cgtacgtgct gaacaccggg atcgtggccc
     3001 gcgacgcgag cgcgcgcccc atctggccgc cgtgggcgga ccgctcgcag gggacgttcg
     3061 tgctcttcgg gctccacgtg aacgacctcg ccgtggatcc ggccaaccgg ctgtgggtgg
     3121 cctcgaacga gggggcctgg ctcatcgagc aggtggaggc gggctacgcc ctggtggagc
     3181 atttcacgga agagaactcg ccgcttttct ccaacgaggt gctctccgtc gccatcgagg
     3241 agcggaccgg cgaggtgttt ttcgccacgg accggggcac ggtatcgtac gcgggtgacg
     3301 cgatcgcccc ggtcgcagag gccgacgacc tgctggtcta tccgaacccg ttccgccccg
     3361 gcgccggcac cggaacggtg actttcgagg ggctggtcga ggagacggac atccgggtgg
     3421 tcacaccggg cggcatcgtg gt
//
LOCUS       DBNHQZ010000425         4734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2565604, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000425 DBNHQZ010000000
VERSION     DBNHQZ010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2565604"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="AC16YU_11495"
     CDS             <1..576
                     /locus_tag="AC16YU_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR6065895.1"
                     /translation="NEVPCVLYGHGVEPVVFQVPEPSLHPLIYTNEFHRVQIKIGRKA
                     AMDCVLKHVDFHPVSDRPMHADFQILKTGEEITLTVPVRYTGTAVGQRDGGRTQAFHH
                     ELEITCMPKDIPDHIEVDISALAIGDTIHLSEITVEGVTFSAAPDTLLFSVVAPRIEV
                     EEVVPVEGEEAAVLGEEGEEGEGEESEGEED"
     gene            601..1167
                     /gene="pth"
                     /locus_tag="AC16YU_11500"
     CDS             601..1167
                     /gene="pth"
                     /locus_tag="AC16YU_11500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6065896.1"
                     /translation="MSKRLIVGLGNPGPEYASTRHNVGFMVADRVAERARVDVRKGRD
                     EALSGWGRWRGHEMGIAKPQTYMNRSGLSVEAMVRRWKLDLADLLVVVDDVHLPLGTL
                     RIRPGGGTGGHNGLQDISDWLDSDEFPRLRFGIGGEFEKGGQSDYVLSPFDPSEEEAV
                     RAAVDRAADACLAFVTDGVQVAMNRFNG"
     gene            1345..3426
                     /locus_tag="AC16YU_11505"
     CDS             1345..3426
                     /locus_tag="AC16YU_11505"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR6065897.1"
                     /translation="MDAALIPYLAPVTGLLALLYAWSRSAWIGRQDPGTPRMVEIAQA
                     ISEGARAFLRQEYRVLVIFVLVLAVLLAVVNGSQPTSSWMIAVSFTVGAFCSGLAGFF
                     GMTVATRANVRTTHAARTGLASALNVAFSGGLVMGLSVVGLGVLGLGTLFLAYDGLTD
                     WDMPRVVGVISGFSLGASSIALFARVGGGIYTKAADVGADLAGKVYEGIPEDDPRNPA
                     TIADNVGDNVGDVAGMGADLFESYVGSIIGTMVLGTVFMGPLTASGGHELAAILLPLA
                     LAGVGILVSIAGSFFVRVKEGGNPQHGLNIGEFGAAGFMAILSYFIIVEVLPAQWTYA
                     GTTYTETGVFWAVIIGLAAGTAIGLVTEYFTATGRGPVNGIARQSITGSATNIIAGLG
                     VGMYSTGIPAVILAAAIMGAHEFAGLYGIAIAAVGMLSVTGIQLAVDAYGPISDNAGG
                     IAEMAELPPEVRGRTDKLDAVGNTTAAIGKGFAIGSAALTALGLFAAYMQQAGVPNID
                     VANPTILAGVLLGATLPYVFSAMAMGAVGRAAGDMIREVGRQFAEIPGLREGTAKADH
                     TRCVAISTKAAIREMIAPGVMAVAVPVIVGFIDKSMLGGLLAGLTVSGVLLAIFQANA
                     GGAWDNAKKTIESTMVVDGKSYGKGSDAHKAAVVGDTVGDPLKDTSGPSLNILIKLIA
                     VVSLVIAPLLA"
     gene            3514..>4734
                     /locus_tag="AC16YU_11510"
     CDS             3514..>4734
                     /locus_tag="AC16YU_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6065898.1"
                     /translation="MAQVEVTMPKMGESITEGTVIVWHKKVGDAVELDEPLLEIGTDK
                     VDTEVPAPAAGVLKEIRVEEGATVDVGSVIAVIDTEGEPAGDGQTTEAGETAEAPAAA
                     AAAPAAAQAAKTAEAPVAKAPAAKAPAAEAPAPEPPAAEAPAAPASGGRVEVVMPKMG
                     ESIMEGTVISWHKKVGDAVELDEVLLEIATDKVDTEVPSPAAGTLVEILAEEGATVEV
                     GAPIAVIAVGEGAAVAEAPKAPAAAKAQAAPAAPAAQEAPAETGPVPRQGEDGRFYSP
                     LVRSIAEKEGVSLFELSGIPGSGSGGRVTKADVLNYLERRAAALAASPVPGAPAASKP
                     SLTVSKPPSEASAAEPEQAASAAARTEIIEMDRMRQIIAEHMTRSKATSAHVTSFAEI
                     DVTNLVRLREAKKDE"
BASE COUNT          711 a         1615 c         1728 g          680 t
ORIGIN      
        1 aacgaggtcc cgtgcgttct gtacgggcac ggcgtggagc ccgtggtctt ccaggtgccc
       61 gagccgagcc tgcacccgct gatctacacg aacgagttcc atcgcgtgca gatcaagatc
      121 ggccgcaagg cggcaatgga ctgcgtgctc aagcacgtgg acttccaccc ggtttcggac
      181 cggcccatgc acgccgactt ccagatcctg aagaccggcg aggagatcac gctcaccgtc
      241 ccggtgcggt acacgggaac ggccgtcggg cagcgtgacg gcgggcgcac gcaggcgttc
      301 caccacgagc tcgagatcac ctgcatgccg aaggacatcc cggaccacat cgaggtggac
      361 atctcggcgc tggcgatcgg cgacacgatc cacctgagcg agatcacggt ggagggggtc
      421 acgttcagcg cggcgcccga caccctgctc ttctcggtgg tcgcgcctcg gatcgaggtc
      481 gaggaggtcg tgccggtcga gggcgaggaa gccgccgtgc tgggcgagga aggcgaggaa
      541 ggcgagggcg aggagtccga aggggaagag gactgacccg ggcccggccc gctcgatgcc
      601 gtgtccaagc gcctgatcgt cgggctcggg aatcccgggc ccgagtatgc ctcgacgcgc
      661 cacaacgtcg gtttcatggt ggccgaccgc gtggcggagc gggcgcgcgt ggacgtgcgc
      721 aagggccgcg acgaggcgct atcgggatgg gggcgctggc gcggccacga gatggggatc
      781 gcgaagccgc agacctacat gaatcggagc ggcctctcgg tcgaggccat ggtgcgccgg
      841 tggaagctgg atctggccga cctcctggtg gtggtggacg acgtccacct cccgctcggc
      901 accctgcgga tccgccccgg cgggggcacc ggcggacaca acggcctgca ggacatcagc
      961 gactggctgg actcggacga attcccgcgc ctgcgcttcg gcatcggcgg cgaattcgag
     1021 aagggagggc aatccgacta cgtgctctcg cccttcgacc cgtccgagga ggaggccgtg
     1081 cgcgcggccg tggaccgggc cgcggacgcc tgcctggcgt tcgtcacgga cggcgtccag
     1141 gtggccatga accgcttcaa cggctgatcg aaaaatgagc aaacaggtgg gatcatcggc
     1201 ctgtttgccc tactttcggg cgcctttcgg cggcacgggc ccggggcccg agtccattcg
     1261 cggaagcgct tgccggacct gcccagcagg ccggcgcttt gttttcgcaa cccactgacc
     1321 cgacgcgttc ctggctctcg accgatggat gctgccctca taccctatct cgccccggtg
     1381 acgggactgc tcgccctgct gtacgcgtgg tcgcgttcgg catggatcgg tcgccaggat
     1441 cccggtactc ccagaatggt ggagatcgcc caggccatct cggagggcgc gcgcgcgttc
     1501 ctgcggcagg agtaccgggt gctggtcatc ttcgtcctgg tgctcgccgt gctcctggcg
     1561 gtcgtgaacg ggagccagcc gacgtcctcc tggatgatcg cggtctcgtt cacggtgggg
     1621 gcgttctgct ccggcctcgc cggcttcttc gggatgacgg tggccacgcg cgcgaacgtc
     1681 cgcacgacgc acgccgcccg gacgggcctg gcctcggcgc tgaacgtcgc gttctcgggc
     1741 ggtctggtca tggggctcag cgtcgtcggc ctcggcgtcc tgggtctcgg cacgctgttc
     1801 ctggcctacg acggcctcac cgactgggac atgccgcggg tcgtgggcgt tatctcgggc
     1861 ttctcgctcg gcgcttcgtc catcgcgctc ttcgcgcgcg tgggcggcgg catctacacg
     1921 aaggccgccg acgtgggcgc cgacctggcc ggcaaggtgt acgagggcat tcccgaggac
     1981 gacccgcgca acccggcgac catcgcggac aacgtgggcg acaacgtggg cgacgtcgcg
     2041 ggcatgggcg ccgacctgtt cgagagctac gtggggtcga tcatcggcac gatggtgctc
     2101 ggcaccgtgt tcatggggcc gctcacggcg tcgggcggac acgagctcgc cgcgatcctg
     2161 ctcccgctcg cactcgcggg cgtgggcatc ctggtgtcga tcgccggctc gttcttcgtg
     2221 cgcgtgaagg agggaggcaa cccgcagcac ggcctgaaca tcggcgagtt cggcgcggcc
     2281 ggcttcatgg ccatcctgtc gtacttcatc atcgtggagg tgctgcccgc gcagtggacg
     2341 tacgcgggca ccacgtacac cgagacgggc gtgttctggg ccgtgatcat agggctcgcc
     2401 gccgggaccg cgatcggcct ggtgacggag tacttcacgg cgaccgggcg cggcccggtg
     2461 aacggcatcg cgcggcagag catcacgggc tccgccacca acatcatcgc ggggctgggc
     2521 gtgggcatgt actcgacggg gatcccggcg gtcatcctcg cggccgccat catgggcgcg
     2581 cacgagttcg cgggcctgta cggcatcgcg atcgcggccg tcggcatgct ctcggtgacg
     2641 ggcatccagc tcgcggtcga cgcctacggg ccgatctcgg acaacgccgg cggcatcgcc
     2701 gagatggccg agcttcctcc cgaggtccgc ggccgcacgg acaagctcga tgccgtcggc
     2761 aacacgaccg cggccatcgg caagggcttt gcgatcgggt cggcggcgct caccgcgctg
     2821 ggcctgttcg cggcctacat gcagcaggcg ggcgtgccga acatcgacgt ggccaacccc
     2881 acgatcctcg ccggcgtcct gctcggcgcc acgctgccgt acgtgttcag cgcgatggcg
     2941 atgggcgcgg tcgggcgcgc ggccggggac atgatccgcg aggtgggccg gcagttcgcc
     3001 gagatcccgg gcctgcgcga gggcacggcc aaggccgacc acacccggtg cgtcgcgatc
     3061 tcgaccaagg ccgcgatccg ggagatgatc gcgccgggcg tcatggccgt cgccgtgccg
     3121 gtcatcgtgg gcttcatcga caagagcatg ctgggcggcc tcctggcagg cctcacggtg
     3181 tcgggcgtgc tgctcgccat cttccaggcc aacgcgggcg gcgcgtggga caacgccaag
     3241 aagaccatcg agagcacgat ggtggtcgac ggcaagtcct acggcaaggg ctcggacgcg
     3301 cacaaggccg ccgtcgtggg cgacaccgtg ggcgacccgc tcaaggacac ctccggcccc
     3361 agtctgaaca tcctgatcaa gctcatcgcc gtcgtttcgc tggtcatcgc tccgctcctg
     3421 gcctgatccg ggcggctgcg tgaaccggcg cccggctccg ccgtaccttg gagcctcgga
     3481 gcgaccccca acagcccagc ccagcacccg gacatggccc aggtcgaagt cactatgccc
     3541 aagatgggcg agtcgatcac ggaaggcacc gtcatcgtct ggcacaagaa ggtgggcgac
     3601 gcggtcgagc tcgacgagcc cctgctcgag atcggcaccg acaaggtgga caccgaggtg
     3661 cccgcgccgg ccgccggcgt gctgaaggag atccgggtgg aggagggggc gacggtggac
     3721 gtgggctccg tgatcgccgt catcgacacc gagggcgagc cggcgggcga cgggcagaca
     3781 accgaggcgg gggagaccgc ggaggcgccc gctgccgctg ctgccgctcc ggctgccgcc
     3841 caggccgcca agaccgccga ggcgcctgtc gccaaggccc cggccgccaa ggccccggcc
     3901 gccgaggcac cggccccgga acccccggcc gccgaggccc ccgcggcgcc cgcttccggc
     3961 ggccgcgtcg aggtcgtgat gcccaagatg ggcgagtcga tcatggaggg gacggtcatc
     4021 tcctggcaca agaaggtggg cgacgcggtg gagctcgacg aggtgctcct cgagatcgcg
     4081 accgacaagg tggacaccga ggtgccctcg ccggccgcgg ggaccctcgt ggagatcctg
     4141 gccgaggaag gtgcgacggt cgaggtgggc gcgccgatcg cggtgatcgc tgtcggggag
     4201 ggggcagccg tggcggaggc gccgaaggct cccgcggccg caaaggcaca ggccgctccc
     4261 gccgccccgg cggcgcagga ggcgcccgcg gagaccggac ccgtgccgcg ccagggcgag
     4321 gacgggcggt tctattcgcc gctcgtccgc tcgatcgccg agaaggaggg cgtgagcctg
     4381 ttcgagcttt cgggcatccc gggatccggc tccggcggac gcgtgaccaa ggcggacgtc
     4441 ctcaactacc tggagcgccg ggccgccgcg ctcgcggcgt cacccgttcc gggcgctccg
     4501 gcggcttcaa agccgagcct gaccgtctcg aagccgccgt ccgaggcgtc ggccgcggaa
     4561 ccggagcagg ctgcctcggc cgcggcccgc accgagatca tcgagatgga ccgcatgcgg
     4621 cagatcatcg ccgagcacat gacgcggtcc aaggcgacgt ccgcccacgt cacgtccttc
     4681 gccgagatcg acgtgacgaa cctggtgcgc ctgcgcgagg cgaagaagga cgag
//
LOCUS       DBNHQZ010000426         4359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1347887, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000426 DBNHQZ010000000
VERSION     DBNHQZ010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1347887"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="AC16YU_11515"
     CDS             complement(<1..306)
                     /locus_tag="AC16YU_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR6065899.1"
                     /translation="MARTFDVPEFYRSRLVTTVKAARRAADPRKRDLSPTLLDFGPVR
                     FRLARHFGFCYGVENAVEIAYRAVAGHPDRRVFLLSEMIHNPHVNDDLRDRGVRFLRT
                     "
     gene            complement(429..1559)
                     /gene="tgt"
                     /locus_tag="AC16YU_11520"
     CDS             complement(429..1559)
                     /gene="tgt"
                     /locus_tag="AC16YU_11520"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6065900.1"
                     /translation="MRFELDHVDSGTGARAGRLVTDHGEIPTPVFMPVGTAGSVKGVD
                     PRELASDLDTRILLGNTYHLYLRPGTDVLRSAGGLHRFMGWDRPILTDSGGYQVFSLS
                     AIRKLTRDGVRFQSHIDGSYHLFTPESVVDVQREIGSDIMMVLDECPPGDATHEYAAR
                     SNDLTIEWAARCKTRFEETGPLYGFDQALFGIVQGVVYPDLRRESARRLVEIGFPGYA
                     IGGLSVGEPAEKTYAMVEVLRDVLPADRPRYLMGVGTPENLVRNVALGVDMFDCVMPT
                     RNGRNGTLFTTEGIVNIRNRKWREHHGPLDPGLDLYASRTFSRAYARHLVMSGEILGL
                     RIASLQNLGLYAWLMREARAAILEDRYGSWMADVLSRVTHRL"
     gene            complement(1562..2680)
                     /locus_tag="AC16YU_11525"
     CDS             complement(1562..2680)
                     /locus_tag="AC16YU_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR6065901.1"
                     /translation="MSRTLRLGLFIVAGATLLMLGLFAIGNRTFLFSDTLTVRTRFTR
                     VAGLQSGAPVQYQGVNVGRVGSVRLPAAPGERIEVSMAISHNAAHLVRENTQAQIKSD
                     GLVGEQIIVLVSTAEPSPAVEPDGYIVGIDPFDLFEITDRALQAVLGFQEAAAAFEQI
                     MTDVQAGEGTLGKIVYDPTLYEELVSTANETRRVMSTLSESAEANAEVLVGLADRVTL
                     GIETILLKVESGDGTVARLINDPGLYESMLASADTLRTIATDLKAVTSVAENAATWGA
                     LGAFRFAELMEAAKHNWLFRRYFEERGTMEQAPFEIRERALAQTYRQIELERRELLLW
                     EERLKEREAALREAGETVPSAESSAPPDSTRAPEGQRP"
     gene            complement(2703..3464)
                     /locus_tag="AC16YU_11530"
     CDS             complement(2703..3464)
                     /locus_tag="AC16YU_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065902.1"
                     /translation="MSPATDIDAIDPVVRLVDLHKAFGATPVLEGITMSVARGSTAVV
                     MGASGSGKSVLIKHVVQLLRPDRGEVWAWDERVDELRGDALDALRLRIGYLFQGGALF
                     DSMSVFENIEFILERHTRMGRRERADRVFELLAWVHLEEKADQAPSELSGGQKKRVAL
                     ARALALEPHLMLFDEPTTGLDPVSVRSVSELIVRLREERGMSSVVITHDLLCAEIVAD
                     RVHFLHGGVFLADGTVDEVRAAEHPEIRNFFGGSR"
     gene            complement(3461..4261)
                     /locus_tag="AC16YU_11535"
     CDS             complement(3461..4261)
                     /locus_tag="AC16YU_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR6065903.1"
                     /translation="MRLTLRPVKRSRALALFFEAIGAFGQFFGHFLARFWRRPFEFRE
                     LLRQMDEVGTRSFALTGITGLAIGIVLAMQTRGTLVRFGAESMLPNMLALSVIKEIGP
                     VLTSLVLAGRLGAGIGAEIGSMRVTEQIDALEVAALKPFHFLVVTRVLACALMFPVLT
                     IWTDAIALAGGYLESLIATEVDYRIFISTAFSALRFSDVLVDTLKTGIFGMIVGLVSC
                     FLGYHVRGGTREVGQAAMQSVVVSSLLILVADVFVVRLSILIFGDISG"
BASE COUNT          721 a         1458 c         1553 g          627 t
ORIGIN      
        1 cgtgcggaga aagcgcacgc cccggtcccg caggtcgtcg ttgacgtgcg ggttgtggat
       61 catctccgag agcaggaaca cccgccggtc ggggtggccg gccacggcgc ggtaggcgat
      121 ctcgacggcg ttctcgacgc cgtagcagaa gccgaagtgc cgcgccaggc ggaagcggac
      181 ggggccgaag tcgagcaggg tcggcgacag gtcccgcttc cgggggtccg cggcccggcg
      241 ggccgccttc accgtggtga cgaggcggct ccggtagaac tcgggaacgt cgaaagtgcg
      301 ggccatcggg ggacggtcga cgggctcggc ggagcccggc cggggagtgc attcggcaac
      361 gcgcctctta cgcgggccgc cgggtcctgt tcgagggccg accgtgcgcg gcccgcggtc
      421 cgcaccgctc acaggcgatg cgtgactcgg gaaagcacgt ccgccatcca ggagccgtac
      481 cggtcctcca ggatcgccgc ccgggcctcg cgcatgagcc aggcgtacag cccgaggttc
      541 tggagcgacg cgatgcgcag ccccaggatc tcgcccgaca tcacgaggtg acgcgcgtac
      601 gcccgggaaa acgtgcgcga cgcgtacaga tccaggcccg gatccaaggg cccatggtgc
      661 tcgcgccact tccggttccg gatgttcacg atgccttcgg tcgtgaagag cgtcccgttg
      721 cgcccgttcc gcgtcggcat gacgcagtcg aacatgtcca caccgagcgc gacgttgcgg
      781 accaggttct ccggcgtgcc gacgcccatg aggtagcggg gacggtccgc gggcagcacg
      841 tcgcggagca cctcgaccat ggcgtacgtc ttctcggcgg gctcgccgac ggacaggccg
      901 ccgatcgcgt agcccgggaa cccgatctcc acgaggcgcc gggccgactc gcgccgcagg
      961 tcgggataga cgactccctg cacgatgccg aagagcgcct ggtcgaatcc gtacaggggc
     1021 ccggtctcct cgaaccgggt cttgcagcgg gcggcccact cgatggtcag gtcgttcgag
     1081 cgcgcggcgt actcgtgcgt ggcgtcaccc ggagggcatt cgtccagcac catcatgatg
     1141 tccgatccga tctcgcgctg cacgtcgacg acggattcgg gcgtgaaaag gtggtacgag
     1201 ccgtcgatgt ggctctggaa gcggaccccg tcgcgcgtga gcttgcggat ggccgagagc
     1261 gagaacacct ggtatccgcc cgaatccgtg aggatggggc ggtcccagcc catgaagcgg
     1321 tgcaggccgc cggccgagcg cagcacgtcg gtgcccgggc gcaggtacag gtggtacgtg
     1381 ttgccgagca ggatgcgcgt gtccaggtcg ctcgcgagct cgcgcgggtc cacgcccttc
     1441 accgatcctg ccgtaccgac cggcatgaac acgggcgtcg ggatctcgcc gtgatccgtg
     1501 accaggcggc cggcgcgggc gccggtcccg gagtcgacgt ggtcgagctc gaaccgcacg
     1561 gtcagggtcg ctggccttcc ggcgcgcgcg tggagtcggg cggagcggag gactcggccg
     1621 aaggaacggt ctcgccggcc tcgcgcaggg cggcctcgcg ctccttcagg cgctcctccc
     1681 aaaggagcag ctcgcgccgc tcgagctcga tctgccggta ggtctgggcg agcgcgcgct
     1741 cgcggatctc gaagggagcc tgctccatgg tcccccgctc ctcgaaatag cgccggaaaa
     1801 gccagttgtg cttggccgcc tccatgagct cggcgaaccg gaaggcgccg agagcgcccc
     1861 aggtggccgc gttctccgcg accgaggtca cggccttcag gtccgtggcg atggtccgga
     1921 gcgtgtcggc ggaggcgagc atggactcgt acaggccggg atcgttgatg aggcgcgcga
     1981 cggtcccgtc gccggattcg accttcagca ggatggtctc gatgccgagg gtcacgcggt
     2041 cggccagccc cacgagcacc tcggcgttgg cctcggcgct ctcggagagc gtgctcatca
     2101 cgcgccgggt ctcgttggcc gtcgagacga gctcctcgta cagggtcgga tcgtagacga
     2161 ttttaccgag cgtgccttcg ccggcctgca cgtcggtcat gatctgctcg aaggcggcgg
     2221 ccgcctcctg gaatccgagc acggcctgga gcgcccggtc cgtgatctcg aagaggtcga
     2281 aaggatcgat gccgacgatg tatccgtccg gctcgacggc cggcgacggc tccgccgtgg
     2341 agacgaggac gatgatctgc tcgcccacaa gcccgtcact cttgatctgg gcctgcgtgt
     2401 tctcgcgcac caggtgggcc gcgttgtgcg agatggccat ggagacctcg atgcgctcgc
     2461 ccggtgcggc cggcaggcgc acgctgccga cccgcccgac gttcacgccc tggtactgga
     2521 ccggggcgcc ggactgaagg ccggccacgc gggtgaaccg cgtccgcacg gtcagcgtgt
     2581 cgctgaacag gaacgtgcgg ttgccgatcg cgaagagccc gagcatgagc agcgtggctc
     2641 cggcgacgat gaaaaggccg agtcgtagcg tgcgagacat gggtcccggg gattgcgggt
     2701 gcctaccgtg agcccccgaa gaagttgcgg atttcgggat gttccgcggc ccggacctcg
     2761 tccaccgtgc cgtcggcgag gaacacgcct ccgtgcagga aatggacgcg gtcggccacg
     2821 atctcggcgc agagcaggtc gtgcgtgatg acgacggagc tcatgccccg ctcctcgcgc
     2881 agccgcacga tgagctccga gacggagcgc accgagaccg gatccaggcc ggtcgtcggc
     2941 tcgtcgaaga gcatgaggtg cggctcgaga gcgagggcgc gggcgagggc cacgcgcttt
     3001 ttctggccgc ccgagagctc cgacggcgcc tggtcggcct tctcctcgag gtggacccat
     3061 gccaggagtt cgaagacgcg gtcggcccgc tcccggcgtc ccatccgggt gtgccgctcg
     3121 aggatgaact cgatgttctc gaacacggac atcgagtcga agagcgcgcc tccctggaac
     3181 aggtagccga tgcgcagacg cagggcgtcg agggcgtcgc cgcgcagctc gtccacccgc
     3241 tcgtcccacg cccagacctc gccccggtcc gggcggagca gctgcaccac gtgcttgatg
     3301 agcacgctct ttcccgatcc cgacgcgccc atcacgaccg ccgtcgagcc acgggcgacg
     3361 gacatcgtga tgccctcgag cacgggcgtc gccccgaacg ccttgtggag gtccaccagg
     3421 cggacgaccg ggtcgatagc gtcgatgtcg gtcgcggggc tcatcccgaa atgtcgccga
     3481 agatgagaat cgagagccgg accacgaaga cgtccgccac caggatcagg agcgacgaca
     3541 cgacgacgga ctgcatggcc gcctggccca cctcccgcgt cccgccgcga acgtggtatc
     3601 ccaggaagca cgacaccagc ccgacgatca tcccgaagat cccggtcttg agcgtatcca
     3661 cgagaacgtc ggagaaccga agggcggaga aggcggtcga tatgaagatg cggtaatcca
     3721 cctcggtcgc gatcaacgat tcgagatacc cgccggcgag cgcgatcgcg tcggtccata
     3781 tggtgagcac cgggaacatg agcgcgcagg cgagcacccg cgtgaccacc aggaaatgga
     3841 acggcttcag ggccgcgacc tccagggcgt cgatctgctc ggtcacgcgc atcgagccga
     3901 tctccgcccc gatgccggcg cccaggcgcc ccgcgaggac gagcgaggtg agcacgggac
     3961 cgatctcctt gatgaccgac agcgccagca tgttcggcag catcgattcg gcgccgaagc
     4021 ggaccagggt gccgcgcgtc tgcatggcca ggacgatgcc gatcgcgagc ccggtgatgc
     4081 cggtcagggc gaagctgcgg gtgcccacct cgtccatctg gcgcagcagc tcgcggaatt
     4141 cgaacggtcg gcgccagaag cgggccagga aatggccgaa gaactggccg aacgcgccga
     4201 tcgcctcgaa gaagagggcc agtgcgcggg agcgctttac gggtctgagg gtcaggcgca
     4261 tcgggtcaga aggcgtgttc ccagcgcagc tgcacgcgca ggatcgctcc cctgctcaag
     4321 agcgaaacca ggagctcgcg caggatctcg tactccatg
//
LOCUS       DBNHQZ010000427         3105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3785265, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000427 DBNHQZ010000000
VERSION     DBNHQZ010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3785265"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16YU_11540"
     CDS             complement(<1..236)
                     /locus_tag="AC16YU_11540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6065904.1"
                     /translation="MGLYSRYVLPLATHRVCSGRPHALQRMNVVPLARGRVLEVGFGS
                     GLNLPYYDTDSVTGVLGLDPSPDMTRMASGRLKDA"
     gene            complement(250..684)
                     /locus_tag="AC16YU_11545"
     CDS             complement(250..684)
                     /locus_tag="AC16YU_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6065905.1"
                     /translation="MSAPRHAWPGGAPSVGQEARRSRTVTARDIELFTEISGDRNPLH
                     YDEALAASTRFGGIVVQGGVTTAILNAVVAEDLPGPGTVFLQTNWRFLAPVRPGDTIT
                     GEVRVRSVREDKPITELDTRVTLADGTVVLDGTAVCYTVPLR"
     gene            complement(784..1542)
                     /locus_tag="AC16YU_11550"
     CDS             complement(784..1542)
                     /locus_tag="AC16YU_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065906.1"
                     /translation="MNPGTQGLAVPAAGAGTGRPTKRFRWFYVAIGFVMTLIVVAGFW
                     PSFFGPLVRGVPGDRHWVLNLHALVFVGWLGLFILQGVLVYRRRKDLHRRLGDYGVAY
                     GVVVLLVGLAATFVVPPLHVASGRWTPDYAAGFLLLPLGDLLLFGGFFVAAVVLKHRH
                     ALHKRLMVLATVALIFPGAARMISPVDAPVLLLAVWLSPLLAAIAFDAVTRKRVERIY
                     IVGGVILVVGFGRILLLESEGWLRIGRAILGLLA"
     gene            complement(1646..2536)
                     /locus_tag="AC16YU_11555"
     CDS             complement(1646..2536)
                     /locus_tag="AC16YU_11555"
                     /inference="COORDINATES: protein motif:HMM:NF018659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HIR6065907.1"
                     /translation="MNARAFALVLVLAVLLVAVRLVLDGGEARDDRAAPPTQEAATLL
                     KLRSVAEPSGVVFHPARRTLFVVDDGGVLCEFTPDGRVVRQALVRDADFEGITVDPST
                     GLLYIAIEGAESLLEVDPETFEALREFALPRAFEGRTLMKAGGQGIEAVTFVPDDAHP
                     HGGTFFVANQSMRLDRIPEDLSVIVEAELPLRSAGAEPGAARILRYFLPGVADIAGLH
                     FDAKRHVILAASDATDRLLVLAPDGRLLASRDLPGQDQEGITVDSTGALFIAQDSGGV
                     LRLEPGWDDPFEGALPDGDF"
     gene            complement(2549..>3105)
                     /locus_tag="AC16YU_11560"
     CDS             complement(2549..>3105)
                     /locus_tag="AC16YU_11560"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR6065908.1"
                     /translation="IPKAGLEATAFHETYPGHHLQMSVALFGKGVHPVLRYLYVASMA
                     EGWGLYSELLADEMDLYSSDLDRLGMLSNQALRAARLVVDPGMHVLGWSRDQAIGYMM
                     DNTAESLAAVESEVDRYLATPAQATAYMTGSLEILRLRAEAEGRLEERFDVRGFHDVI
                     LADGAVTLPMLRRAVERWADGLQD"
BASE COUNT          516 a         1064 c         1097 g          428 t
ORIGIN      
        1 gcatccttca gtcgtccgga ggccatgcgg gtcatgtccg gggagggatc gaggcccagc
       61 actccggtca ccgagtccgt gtcgtagtac gggaggttca gtccggatcc gaatccgacc
      121 tcgaggaccc gcccgcgcgc gagcggcacc acgttcatgc gctgcagcgc gtggggcctg
      181 ccgctgcaga cccggtgagt ggccagggga aggacgtatc gggagtagag ccccattccc
      241 cgcgaggtat cagcgaagcg gaacggtata acatacggcc gtgccgtcga gaaccacggt
      301 gccgtcggcc agggtcaccc gcgtgtccag ctccgtgatc ggcttgtcct cgcgcacgct
      361 ccgcacgcgc acctcgccgg tgatcgtgtc tcccggccgg accggcgcca ggaaacgcca
      421 gttcgtctgc aggaagaccg ttcccggccc gggcaggtcc tcggccacca cggcgttcag
      481 aattgccgta gtgacacccc cctgcacgac gatgccgccg aaacgggtcg acgcggccag
      541 cgcttcgtcg tagtgcagcg ggttgcgatc gcccgagatc tcggtgaaga gctcgatgtc
      601 gcgcgccgtc accgtgcgcg agcggcgagc ctcctgcccg accgacgggg caccgcccgg
      661 ccaggcgtgc cggggtgcgc tcatccggcc gtcctccgga gtcggtccgc cccggcgtcc
      721 gcctccatgg tgctggccgg cacggaggtc cccggtacga tgggttcacc ggtcatggtc
      781 agatcaggcc aggaggccca ggatggcccg tccgatccgg agccagccct ccgattcgag
      841 gagcaggatg cggccgaacc cgacgaccag gatgaccccg ccgacgatgt agatccgctc
      901 gacccgcttc cgcgtcacgg catcgaacgc gatcgcggcg aggagcggcg agagccacac
      961 tgcgagaagg agaacgggtg cgtcgacggg cgatatcatg cgcgcggccc cggggaagat
     1021 gagggcgacc gtggcgagga ccatcagccg cttgtgcagc gcgtgccggt gcttcaggac
     1081 gacggccgcg acgaaaaatc ctccaaagag caggagatcg ccgagcggca ggagcaggaa
     1141 gccggccgcg tagtccggag tccaccggcc ggaggcgacg tgcagcggcg ggaccacgaa
     1201 cgtcgcggcc agacccacca gcagcaccac gacgccgtac gcgacgccgt agtcgccgag
     1261 gcggcggtgc aggtccttcc tcctccgata cacgagcacg ccctggagaa tgaacaggcc
     1321 gagccagccg acgaacacca gcgcgtgcag gttgagcacc cagtgccggt cgcccgggac
     1381 gccccggacc agcggcccga agaagctcgg ccagaagccc gccacgacga tgagggtcat
     1441 gacgaagccg atggccacat agaaccatcg gaaacgcttc gtcggccggc ccgtgccggc
     1501 gccggccgcc ggtacggcga ggccctgggt gccaggattc atcgatgttc cgggttcgtg
     1561 ggtcggcccg tgcgggggtg atcgccccgg cgggaggggg tgcttcgagc ggggcggccc
     1621 atcttacggc gctccccgtt tccggtcaaa agtcgccgtc cggcaaagcg ccctcgaacg
     1681 ggtcgtccca gccaggctcc agccggagca cgccgcccga gtcctgcgcg atgaagagcg
     1741 cgcccgtcga atccaccgtg atgccttcct ggtcctgtcc cggaaggtcc cgtgacgcga
     1801 gaagccggcc gtccggagca aggaccagga gccgatccgt cgcgtcgctc gcggccagga
     1861 tcacgtggcg cttggcgtcg aagtgcaggc ccgcgatgtc ggccacgccc ggcaggaagt
     1921 atcggaggat ccgcgccgca cccggttccg cgcccgcgga acgcagcgga agctcggcct
     1981 cgacgatgac cgagagatct tcaggaatcc tgtcgaggcg catggactgg ttggcgacga
     2041 agaacgtccc tccgtgggga tgcgcatcgt ccggcacgaa ggtgacggcc tctatgccct
     2101 ggccacccgc cttcatcaga gtccggccct cgaaggcccg cgggagtgcg aattcccgga
     2161 gcgcttcgaa ggtctccgga tccacctcca ggagggattc cgccccttcg atcgcgatgt
     2221 agagcaggcc ggtcgacgga tcgacggtga ttccctcgaa atccgcgtcc cggacgaggg
     2281 cctggcgcac cacgcgtccg tccggcgtga attcgcacag cacgccgccg tcgtcgacca
     2341 cgaaaagggt ccttcgagcc gggtggaaga cgacgccgga aggctccgct accgagcgga
     2401 gtttcaggag cgtcgccgct tcctgcgtgg ggggggcggc ccggtcgtcc cgggcctcgc
     2461 cgccgtcaag gacgagtcgg acggccacga ggagcacggc caggacgagc acgagggcga
     2521 aggcgcgggc gttcatccgt cttcggggtc agtcctgaag gccgtccgcc cagcgctcca
     2581 cggcccgccg gagcatgggc agcgtcacgg cgccgtccgc gaggatcacg tcgtggaagc
     2641 cccggacgtc gaagcgctct tcgagccggc cctccgcctc ggcgcgcagg cgcaggatct
     2701 ccaggcttcc ggtcatgtag gccgtggcct gcgccggcgt cgcgaggtag cggtcgacct
     2761 cggactcgac ggcggcgagt gattcggccg tgttgtccat catgtatccg atggcctggt
     2821 cgcggctcca gccgagtacg tgcatgccgg ggtcgacgac gagccgggcc gcgcgcagcg
     2881 cctggttgga cagcatcccc aggcgatcca ggtccgacga gtaaaggtcc atctcgtcgg
     2941 cgagcagctc cgagtagagg ccccagcctt ccgccatcga cgcgacgtac aggtatcgga
     3001 gcacgggatg cacgcccttc ccgaaaaggg cgacggacat ctgcaggtgg tgcccggggt
     3061 acgtctcgtg gaaggccgtg gcttcgagtc cggccttcgg gatgg
//
LOCUS       DBNHQZ010000428         4263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3105419, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000428 DBNHQZ010000000
VERSION     DBNHQZ010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3105419"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1090)
                     /locus_tag="AC16YU_11565"
     CDS             complement(170..1090)
                     /locus_tag="AC16YU_11565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6065909.1"
                     /translation="MDEHDPEKRIRLLERKLERSERNRAELEEQRSKGQLLYRRLHAD
                     LAAAQAQLVQAEKLASLGELTAGIAHEIKNPLNFVNNFAEVNLELVQELAEALRTVGA
                     YDRVADLLNDLRTNSEAVQRHGRRADSIVRTMMQHASGGTGEKTRTDLNALVNEHIDL
                     AYHGRRAHASGFQVSIVRDLADDAGSVDIVPQSIGRVLLNLLGNAFDAVQEHAAAAGS
                     GFAAEIRVFTRRHPGRVEIRVADNGPGMPEDVRDRIFEPFFTTKPTGSGTGLGLSLSY
                     DIVTSGHGGSLTVESEPGRGATFVVSLPVG"
     gene            complement(1093..2232)
                     /locus_tag="AC16YU_11570"
     CDS             complement(1093..2232)
                     /locus_tag="AC16YU_11570"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR6065910.1"
                     /translation="MKTTIHHSTDIDSDEAIAEIMAECESAWTDSVPDAGFLFASTDH
                     DLEVLQRPVMERWPELQLVGCSGNGQISSLRGTQDDSVVLLLFWSDQVRFRAGLGREV
                     SRDPVAAARQAVSEATEGEAHAPALCVLLPEGWKVDIRVVLETVQGMLGPETLVVGGL
                     SDTETHDRPTRQAFRGAALSDAVTCLCFFGPLRTSSGVRSGMRPVGRKHRVTSASGPY
                     VHTIDGRPAESLWTEYVGAHSLWYPLAVFPEDGDQFYLAASPVVTDDGSAYFFNPIPE
                     GATVQMADVTQDEIIAAVAESCDAAVAGFGEAVPSAALVFSCSGRKGMLGTRVQDEID
                     LLRSRLDARIPIAGFYTAGELCPLPGQATTRDHGFTFVAVLLGEE"
     gene            complement(2236..3384)
                     /locus_tag="AC16YU_11575"
     CDS             complement(2236..3384)
                     /locus_tag="AC16YU_11575"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR6065911.1"
                     /translation="MLAVVGHSEDIDSADAIAEVMASSADRLGGAVPQAGLLFAGVDR
                     DFAAMQDAVLARWPGLQLIGGSSLGEVSSVEPATEDSVALMLFAPDRVRIRAGIGTGV
                     SRDPDAAARAAVAMACGGDAEDPSLCLLLGPGLWVDTLALVSAVQDALPPGTRLVGGM
                     TADRRGKAERTIQMGCGEVSDDAACVLTFHGPLAVSHAVRSGWLPLGPFHRITRSVGN
                     VVETINDRPALDLYVEYFGGHSVDFPLAIYPHEADDDSFFLNVALQTEENGGLAFNQH
                     IAEGARVRLADARAPEIIDAAEAVAREAVGAFPDARPEVGIVVSCAGRMFALGTRTRE
                     EVDRLRGIVGLDMPLIGFYSNGEVCPLAGQREARLHNCTFVSLLLGET"
     gene            3672..4040
                     /locus_tag="AC16YU_11580"
     CDS             3672..4040
                     /locus_tag="AC16YU_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6065912.1"
                     /translation="MSNKQKMVVVVTNGVDNERSSVAWSVANGGVGMGFEVTMFLVSS
                     GVDWARKGAAEKARPNPLDPSIGEMMRNVMDNGGQVLVCPPCANVRGYTEDALIDGVT
                     VAGSAAMLGVAAQGAITLSF"
BASE COUNT          678 a         1476 c         1485 g          624 t
ORIGIN      
        1 gcggcaagga ggacgtgacg catggccttt ccgacagtct ggatgtgagg gatgacccgg
       61 ggccgtgtgg ggactgtact gtcggggcgt cggataatca aggggccggc tcgcgacccg
      121 atgacatccc ctcgagccgt gtcctgcagc ccccggacgc gtctccccct caccccaccg
      181 gcagcgatac gacgaacgtc gcgccccggc ccggctcgct ctcgaccgtg agactgcccc
      241 cgtggccgct ggtcacgatg tcgtacgaga gcgacaggcc gaggccggtt ccggaccccg
      301 tgggcttcgt cgtgaagaac ggctcaaaga tccggtcccg gacgtcctcc ggcatccccg
      361 gcccgttgtc ggcgacacgg atctcgacgc gcccggggtg ccggcgggtg aagacgcgga
      421 tctccgcggc gaagcccgat ccggcggctg cggcgtgctc ctggaccgcg tcgaacgcat
      481 tgccgagaag gttcaggagg acgcggccga tcgactgcgg cacgatgtcg accgatccgg
      541 catcgtcggc caggtcgcgg acgatggaga cctggaagcc cgatgcgtgg gcacgccgtc
      601 cgtggtaggc caggtcgatg tgctcgttca ccagcgcgtt caggtcggtc cgcgtcttct
      661 ctcccgtgcc gcccgacgcg tgctgcatca tggtccgcac gatggaatcg gcgcgtcggc
      721 cgtgcctctg cacggcctcg gagttcgtgc gcaggtcatt caggaggtcg gccacccggt
      781 cgtaggcccc gacggtgcgc agggcttccg cgagttcctg gaccagctca aggttcacct
      841 cggcgaagtt gttgacgaag ttgagcgggt tcttgatctc gtgggcgatc ccggccgtga
      901 gctcgccgag ggaggcgagc ttttccgcct gcacgagctg ggcctgggcc gcggcgaggt
      961 cggcgtgcag gcggcggtac aggagctggc ccttggagcg ctgctcctcc agctcggcgc
     1021 ggttgcgctc ggaacgctcg agcttccgct ccaggagccg gatccgcttt tcggggtcgt
     1081 gctcgtccat cgtcattcct cgcccagcag cacggccacg aaggtgaaac cgtgatcccg
     1141 cgtggtcgcc tggcccggca gggggcacag ctcgccggcc gtgtagaacc ccgcgatcgg
     1201 aatgcgggcg tccagccggg accggaggag gtcgatctcg tcctggaccc gcgtcccgag
     1261 catgcccttg cggccggagc acgagaagac cagcgccgcc gacgggaccg cttctccgaa
     1321 gccggcaacc gccgcgtcgc aggactcggc caccgcagcg atgatctcgt cctgggtcac
     1381 gtcggccatc tggaccgtcg ccccttcggg gatgggattg aagaagtacg cagagccgtc
     1441 gtcggtcacc acgggcgacg cggccaggta gaactggtcg ccgtcctccg ggaagaccgc
     1501 cagcggatac cacaacgagt gcgcccccac gtactcggtc cagagcgact cggcaggccg
     1561 gccgtcgatc gtgtgcacgt aaggcccgga ggcggacgtg acccggtgct tgcgcccgac
     1621 cggcctcatg ccgctccgga ccccgctgga cgtccggagc gggccgaaga agcagagaca
     1681 ggtcacggcg tcggagagtg ccgctccgcg gaaggcctgg cgggtcggcc ggtcgtgcgt
     1741 ctccgtgtcg gagagaccgc cgacgaccag cgtctccgga cccagcatgc cctgcacggt
     1801 ctcgagcacg acgcggatgt cgaccttcca cccctccggc aggagcacgc acagggcggg
     1861 cgcgtgggcc tcgccctcgg tggcttcgga caccgcctgc ctcgccgcgg cgaccggatc
     1921 gcgagagact tcccgtccga ggcccgcgcg gaagcgcacc tggtccgacc agaacagcag
     1981 cagcaccacc gaatcgtcct gcgtgccgcg cagggacgag atctgcccgt ttccgctgca
     2041 cccgacgagc tgcagctcgg gccagcgctc catgaccggc cgctgcagca cctcgagatc
     2101 gtgatcggtg gaggcgaaca ggaaaccggc atccggaacg gaatccgtcc aggccgactc
     2161 gcattccgcc atgatctcgg cgatcgcttc gtccgagtcg atgtccgtgc tgtggtggat
     2221 cgtcgtcttc atggatcagg tctcccccag gagcagcgag acgaacgtgc agttgtgaag
     2281 ccgcgcctcc cgctgccccg ccagcggaca gacctcaccg ttcgagtaga atccgatgag
     2341 cggcatgtcc aggccgacga tacccctgag ccggtcgacc tcctcgcggg tccgcgtccc
     2401 gagggcgaac atccggcctg cgcaggagac cacgatgccc acttcgggac gggcgtccgg
     2461 gaaggcgccg accgcctcgc gggccaccgc ctcggcggcg tcgatgatct ccggggcccg
     2521 ggcgtcggcc aggcggacgc gcgcgccctc cgcgatgtgc tggttgaacg cgaggccgcc
     2581 gttttcctcg gtctgcaggg cgacgttcag gaagaacgaa tcgtcgtcgg cctcgtgcgg
     2641 atagatggcg agcggaaagt ccacggagtg gccgccgaag tactcgacgt acaggtcgag
     2701 cgcgggacga tcattgatgg tctcgacgac gttgccgacc gaccgggtga tccggtggaa
     2761 gggtccgagc ggcagccatc cgctgcgaac ggcgtgggag acggcgagcg gaccgtggaa
     2821 cgtcaggacg cacgctgcat cgtcggaaac ctcgccgcag cccatctgga tcgtccgctc
     2881 ggccttgccg cgccggtccg ccgtcatccc gcccacgagc cgggtgcccg gaggaagcgc
     2941 gtcctggacg gcgctcacca gcgccagggt gtccacccag agaccgggac cgagaagaag
     3001 gcacaggctc gggtcctccg catcgccgcc gcaggccatc gccaccgcgg ctcgggctgc
     3061 ggcgtccggg tccctcgaaa cgcccgtccc gatcccggcc cggatgcgca cgcgatccgg
     3121 cgcgaagagc atcaatgcga ccgagtcctc ggtcgccggc tccacggacg agacttctcc
     3181 gagggaggaa cctccgatga gctggaggcc gggccagcgc gcaaggacgg cgtcctgcat
     3241 cgccgcgaag tcgcgatcga cgccggcgaa caggagtccg gcctgcggga cggcgccgcc
     3301 gagccggtcg gcgctggacg ccatcacctc ggcgatggca tcggcggaat cgatgtcctc
     3361 gctgtgtccg acgacggcga gcatggggtt ctggagtgcg ggggcaccga tgatacgaaa
     3421 gcgagtcgag agcaacgggc accggcgacc actcgccggc caccgatgca ttcccgggga
     3481 gcgaactccc gccgcgagcc gcccgcccgg gactggcgtc aagatttctt tcgcctcgat
     3541 ggacctggat ccgggcggat ggcgtcagcc tgattggatg ggtcgcacgc cgggaatcca
     3601 gacccccgga tcgacggcgt ctcccatccc gcttttccat gacagctcaa tcacagagag
     3661 gtaaacgaat catgtcgaac aagcagaaga tggtcgtcgt cgtcacgaac ggtgtggaca
     3721 acgagcggag ctccgtggcc tggtccgtcg cgaacggcgg cgtgggcatg ggattcgagg
     3781 tcaccatgtt ccttgtcagc tccggcgtgg actgggcgag aaagggcgca gcggagaagg
     3841 cccgcccgaa cccgctcgat ccgtccatcg gggaaatgat gcggaacgtg atggacaacg
     3901 gcggccaggt cctggtctgc ccgccctgtg ccaacgtccg cggctacacc gaagacgcac
     3961 tgatcgacgg cgtcaccgtg gccggatcgg ccgccatgct cggcgtcgcc gcccagggcg
     4021 ccatcacgct ctccttctga cccggcccgg tcgaaggtcg ccggagcgac gctccgtccg
     4081 ggtcgcggcg tggcgcactg cgccccgggc cgctaccttc gcgccaaccc tgcggaagga
     4141 tgacagaccg cgaccccgtt caccgcgcat tcagtgagca cgtggaggcg cagctcgacg
     4201 ccctgtacgg cgtcgcgctg cgcctcgcgc gtgacccggt ggacgccgaa gatctcgtgg
     4261 ccg
//
LOCUS       DBNHQZ010000429         1952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_262050, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000429 DBNHQZ010000000
VERSION     DBNHQZ010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_262050"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1470)
                     /locus_tag="AC16YU_11585"
     CDS             complement(175..1470)
                     /locus_tag="AC16YU_11585"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR6065913.1"
                     /translation="MVLVAAALGVAPVRAQEAVPEFFVDAVVTRGAGVSHQSRVDLYL
                     RIPFERLSFGSSIDGFTARYEVSVEALTRAGDGLTSTPVRSRTWDATFRVPTYAETTN
                     PSAFVSTTQAIDLDPGSYQLSVQVRDLASGESFVRDLPLEVRDMNRPHAISDLTLLES
                     YDPSTLSIVPRADNVVGTEEPGFDLFCEVFSSAPRQVRIHHQVLRIHKDGGIHRIPAE
                     GLDPASGEVVSERSAVVDLTTGRSQHVVHVPVEDMKVGTYGARAWIEGADGTVIDETF
                     KIVQVRWTGLAAHIQDVDLAIEQLEYIANRKDLQHIRSAPTRAERYARFRAFWDRRDP
                     TPGTRRNESMEEYYYRVNFANRRYSAVTDGWKTDRGFVLVKFGEPDIVERRPHSFDFE
                     PYEVWVYQRMGRQYIFVDKTGFGDYQLLVPVWDERTRLY"
     gene            complement(1723..>1952)
                     /locus_tag="AC16YU_11590"
     CDS             complement(1723..>1952)
                     /locus_tag="AC16YU_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6065914.1"
                     /translation="DASTGQNAIRQAEEFTRSVDVTGLVLTKLDGTAKGGIVIGISNE
                     FGIPVKYIGVGERIEDLQVFDRQRFVEALFR"
BASE COUNT          334 a          671 c          643 g          304 t
ORIGIN      
        1 ccggtccctg aggggccaac cccgccgcgg gcgggcttga ccctgcgggc tcggacacac
       61 gaaccggctg ccctccctca tcagtacagc cgcgtccgtt ccctgagggg ccaaccccgc
      121 cgcgggcggg cttgaccctg cgggctcgga ctcacgaacc gtctgcccct ctcatcaata
      181 gagccgcgtc cgttcgtccc acacggggac gaggagttgg tagtcgccga acccggtctt
      241 gtccacgaag atgtactggc gccccatgcg ctggtagacc cagacctcgt acggctcgaa
      301 atcgaatgaa tggggccgcc gctccacgat gtcgggctcg ccgaacttga cgagcacgaa
      361 gccccggtcc gtcttccacc cgtcggtcac ggccgagtag cggcggttgg cgaagttgac
      421 ccggtagtag tactcctcca tgctctcgtt gcgccgcgtg cccggggtcg ggtcgcggcg
      481 gtcccagaac gcacggaacc gcgcataccg ctcggcgcgg gtcggagcgg agcggatgtg
      541 ctgcagatcc ttgcggttgg cgatgtactc gagctgctcg atcgccaggt ccacgtcctg
      601 gatgtgcgcg gcaagcccgg tccagcggac ctgcacgatc ttgaaggtct cgtcgatcac
      661 ggtgccgtcc gccccctcga tccatgcccg ggcgccgtag gtgcccacct tcatgtcctc
      721 gacgggcacg tgcacgacgt gctggctgcg ccccgtcgtg aggtcgacga cggccgagcg
      781 ctcggagacg acctcgcccg atgccgggtc cagtccctcc gcggggatcc gatggatgcc
      841 gccgtccttg tggatccgca gcacctggtg gtggatccgc acctgccggg gcgccgacga
      901 gaacacctcg cagaacaggt cgaaccccgg ctcctccgtc ccgaccacgt tgtcggcccg
      961 cgggacgatg gagagtgtcg aggggtcgta cgactcgaga agcgtcaggt cgctgatcgc
     1021 gtggggccgg ttcatgtccc tgacctcgag aggcaggtcg cggacgaacg actcgccgga
     1081 cgccaggtcg cggacctgca cggagagctg gtaggagccg gggtcgagat cgatcgcctg
     1141 ggtcgtggaa acgaaggccg acggattcgt ggtttccgcg taggtcggga cccggaaggt
     1201 cgcgtcccac gtccggctcc ggacgggagt ggaggtcagc ccgtcgccgg cgcgggtaag
     1261 ggcctcgacc gagacctcgt accgcgcggt aaatccgtcg atcgaactcc cgaacgagag
     1321 ccgttcgaac ggaatgcgca ggtacaggtc cacccgggac tggtgggaca cgcccgcccc
     1381 gcgggtgacg acggcatcca cgaaaaactc cggcacggcc tcctgcgccc gtaccggagc
     1441 aacgccgagt gccgccgcaa cgagaaccat gctccagatc cgcacccggt ggcccgcttt
     1501 gtggcacata cagtagcgat tcgtcatacg gtcgatggga agtggaaccc cgacggcccg
     1561 gccgtcgcct cacaaacgga atatcgcaag atcggcggag agcgtcccgc gagccgtcat
     1621 cgacggtaga atgccgacga cgaacggtaa gccacgcaaa gcgaaccgga ggcttgcgaa
     1681 gccggcccga ggctcgcggc gtgttccggg catccgcgag ccctagcgga acagggcttc
     1741 cacgaaacgc tgccggtcga agacctgcag atcctcgatg cgctcgccga ccccgatata
     1801 ctttacggga attccgaact cgttcgaaat gccaatcacg atccccccct ttgcggtccc
     1861 gtcgagcttc gtgagaacga gcccggtaac gtccacgcta cgcgtgaact cctccgcctg
     1921 gcggatcgcg ttctgccccg tggacgcgtc ca
//
LOCUS       DBNHQZ010000430         7954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3380178, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000430 DBNHQZ010000000
VERSION     DBNHQZ010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3380178"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16YU_11595"
     CDS             complement(<1..274)
                     /locus_tag="AC16YU_11595"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844576.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR6065915.1"
                     /translation="MSTHDPFGARDTFDTGSGTGIVYRLDRLADHGFRHLDRLPFSIK
                     VLLESVLRNCDDYVYTRDHVAMLADYDPKAPAREEIPFMPARVLLQD"
     gene            540..612
                     /locus_tag="AC16YU_11600"
     tRNA            540..612
                     /locus_tag="AC16YU_11600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Ala,seq:cgc)
     gene            complement(718..1749)
                     /locus_tag="AC16YU_11605"
     CDS             complement(718..1749)
                     /locus_tag="AC16YU_11605"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6065916.1"
                     /translation="MQDTPSTPDSRPPSRRTFLAAAARSAAGLALVGAYPGWLWLQGR
                     RQALSRRSAWSMGTSVSLTSPREAESADAVRRAFASLARVQDRLSVHDPASALSTLNR
                     EPGIWMDADEDLLAVARAARALGERTGGALDVTVLPVLRRLGFRPGEPAGAGLERIGF
                     EHLRVEGSRVSLDTGGYGVDFGGIAKGYAVDLGVEATRAAGSGPVLLEAGGDLFAAGR
                     PEPGSRWTIGVRDPVRVQGIAARFEVEDEAVATSGTYFQRRTVDGRSVSHLIDPRTGA
                     PSERVLSSTIVARDCMTADALATATAVMEPDAALDLAQSLPGVEGLWIYPDRSYRATP
                     GLASRLEWV"
     gene            2087..2671
                     /locus_tag="AC16YU_11610"
     CDS             2087..2671
                     /locus_tag="AC16YU_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HIR6065917.1"
                     /translation="MLRTLIPAIALVALAFSGRAVQPEPVAEPDGHAWLVQDHQFIGA
                     TRCRTCHRKEEDGAQFDKWAASAHASAYTTLASDQAKAIAAEKGIEDPQKAAECLVCH
                     TTGHGVPAEMLGDKYDIAEGVTCEACHGAGGDYYKKATMEGIAAGDIEGASVGLVMPT
                     AETCITCHNEKSPTFKAFNYEEQVAKIAHPNPNN"
     gene            2736..3929
                     /locus_tag="AC16YU_11615"
     CDS             2736..3929
                     /locus_tag="AC16YU_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065918.1"
                     /translation="MPERSSAQFRYDGQVYAMAYAWEAPADESGRRFDTYEGLRLRVR
                     PGDALDVRTHVLFARRSRMDAAGSLSYEWEERVYQLYADARLSGRELTGRLGRQFVYR
                     GTINGTFDALALRYRPRADWDVQALAGFAAPYARDLDFTPASDDENLWDERGSLGLFV
                     SKTFGRRIRANASWFQRHRSGDIAWSLAGVGFSAGYGHGRVVQAEVEYNTEQESIQRM
                     RVRDWGEIGRWILSIEASTQRPRIFEDSFFSRFSVTGYGQVRLGLSYDLGSGRAGYEF
                     VGTDFEGDQRSGEHILTYGLDQGRFSGMLGFVYQAGYGGERISPYVDARYELLDGVTL
                     RGNFTYLEYERRTISLDEDATAWTAGVLVRPVSRWWLQLDVQQAANTFYDQDTRLLAR
                     AGVSF"
     gene            3952..4866
                     /locus_tag="AC16YU_11620"
     CDS             3952..4866
                     /locus_tag="AC16YU_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6065919.1"
                     /translation="MKVLAAVLFVPATFFWQMAVMPMALPGSIDGGVRFSHRLHMEDA
                     GTACADCHEPAAESMTGADNLLPDHDVCSACHEVEEPDECGTCHTDADDPEGLVYITA
                     YSTLFPHATHLAGGFECADCHAGIEAAEPDDDVELPGMARCVDCHNEEVASVECRTCH
                     EAGEDLMPATHVVGFARTHGPQAAQNAISAHGDLTCQTCHDDVFCQDCHEGENLDRLS
                     HPLNYEFTHALDVQSGAVNCTTCHTETAFCVDCHAENQLMPQTHTAGWVNALDGGRHR
                     IEALNDLDTCIACHEDDAEITCGGCHVD"
     gene            4856..5836
                     /locus_tag="AC16YU_11625"
     CDS             4856..5836
                     /locus_tag="AC16YU_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain c-type cytochrome"
                     /protein_id="HIR6065920.1"
                     /translation="MSTRAFGIAARLSAALGTVLFVAFAVGCADLADEPEDYYHPAEW
                     MVSASADFHGAAVLASPGKAESCQTCHGETYGGGTSGVACTTCHAAYPHEAAFGGSPR
                     PTHAAFMREQAGWDLSMCQSCHGAAYDGDGVASKTCLTCHDGDGGPEACNTCHGDDAT
                     GVPPPDLLGNEASTFRGVGAHEVHAALGTGSEACLSCHAVPASYGAAGHIDDDPARAE
                     IVFGGLAVHAGAEPSYDASAGTCGSTYCHGGFSFPKEGSPYAFAYTGDFITGNGPEVE
                     WTGGEAEATCGSCHGLPPTGHINNAACTNCHNTFPANHIDGKINVFGSSR"
     gene            6083..>7954
                     /locus_tag="AC16YU_11630"
     CDS             6083..>7954
                     /locus_tag="AC16YU_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6065921.1"
                     /translation="MTTPRGLLIVLAGFLLMPVTAAVAQEGDDAECMACHDDRSLIVE
                     RDGRRVSMHVNYQAFAQSVHAREGCISCHADVDVDDLPHAEDLDRVDCSMCHDDEVEQ
                     FTASLHGQAAHSNRFLAPSCTTCHGKHDILSSKNRAARTYVTNIPSLCGECHKDGTRV
                     SELRAISQRHILEDYAESIHGDGLFRRGLIVTAVCTSCHFAHNILPHENPNSSINQAN
                     IASTCQQCHAKIEEVHVKIIDGELWEKRPHEVPICVDCHQPHRVRRVDYTASFPDRRC
                     LDCHSNPDLSVTEADGSMRPLFVDPDDLLHSSHQNQSCISCHTSVTESRDPVCLDSGP
                     VDCAMCHAEQVDQFAISTHGQKVASGDERAPYCTTCHGDHRVLAPDNPESPVFPRNLP
                     DLCAGCHENRVEPILTNARDGDNILTDYSGSIHGKGLLQSGLLVTATCVDCHTAHMEL
                     PISDTLSTVHPSNVASTCARCHDGIMEQFNRSVHSPLANDTDQKLPACNDCHRSHTIR
                     RTDAGDFRQDMLNQCGDCHAEVTETYFDTFHGKVSQLGSEVTAKCSDCHGAHNILPPT
                     DPESMLSRQNVVETCGKCHPSSNRQFVGYLTHATHHDRDKYPYLYYTFWFMTSLLVGV
                     "
BASE COUNT         1291 a         2762 c         2746 g         1155 t
ORIGIN      
        1 agtcctggag gaggacgcgc gccggcatga acggaatctc ctcccgggcg ggcgccttcg
       61 ggtcgtagtc ggcgagcatc gcgacgtggt cccgcgtgta cacgtagtcg tcgcagttcc
      121 ggagcacgga ctcgagcagg accttgatgg agaacggcag gcggtcgagg tgcctgaaac
      181 cgtggtcggc cagccggtcc agccggtaga cgatgccggt cccggaaccg gtgtcgaacg
      241 tgtcgcgggc gccgaagggg tcgtgcgtgc tcatggtcat gcgggtcgat tcgaggacgg
      301 gtcgtgcggg cgggccgacg ccgcggccgc gggttccgaa gatacccgct ggctccggac
      361 attgttcgtc ccggggcggc aacgcggccg gccgtcatgg ccgaaaagcg gtgaaatcca
      421 cccgcgccgg gtgcctcacc gggtgccgga cgggagccgc cttgaacggg ccgagggacc
      481 gggcgtatat ttgaagtctg cggaaccggg tccggtgggc gacgtttcgc ggccgattcg
      541 gggctatagc tcagttggta gagcgcttgg atcgcaccca agaggtcagg agttcgaatc
      601 tccttagctc cacggggcgg ctcttcaaag ggggagccgc ccgtttcatt tccggcagcc
      661 cccgccgacc atccgcagtc ccgatcgtcc gggaccggga cgggtccgcc ggttccgtca
      721 gacccactcg aggcgcgagg ccagacccgg cgtggcccgg taggaccggt ccgggtagat
      781 ccacagtccc tcgacaccgg ggagcgactg cgcgaggtcg agcgccgcgt ccggctccat
      841 gacggcggtg gccgtcgcga gggcgtcggc cgtcatgcag tcgcgggcga cgatcgtcga
      901 gctgaggacc cgctcggagg gcgcaccggt gcgggggtcg atcaggtgcg acaccgaccg
      961 gccgtccacc gtccgccgct ggaagtacgt tcccgaggtg gccacggcct cgtcctcgac
     1021 ctcgaaccgg gccgcgatgc cctgcacccg cacgggatcc cgcacgccga tcgtccagcg
     1081 agagccgggc tccggccggc cggcagcgaa gaggtcgcct ccggcctcca gcagaacggg
     1141 tcccgatccg gcggcgcgcg tggcctccac gcccagatcc acggcgtagc ccttcgcgat
     1201 gccgccgaag tccaccccgt atccgcccgt gtccaggctc acccggctgc cctcgacgcg
     1261 caggtgctcg aaaccgatgc gctccaggcc cgcaccggcg ggctcaccgg gccggaagcc
     1321 caggcgcctg agcacgggaa gcacggtgac gtcgagcgcg cccccggtcc gctctccgag
     1381 cgcacgggcg gcgcgcgcga cggcgagaag gtcctcgtcg gcatccatcc agatcccggg
     1441 ctcccggttc agcgtgctga gcgcggaggc gggatcgtgg accgagagcc ggtcctgcac
     1501 gcgggccagc gatgcgaagg cgcgtcgcac ggcgtccgcc gactccgctt cgcgaggcga
     1561 ggtcagcgac acggaggtcc ccatggacca ggccgaccgg cgggagagcg cctggcgacg
     1621 cccctggagc cacagccacc ccgggtaggc gcccacgagg gcgaggcccg cggccgagcg
     1681 ggcggcggcc gccaggaacg tccgcctgga cggcggtcgt gagtcgggag tagacggagt
     1741 gtcctgcatg gtggacgcgg agtgacggga tttggcggcc gaaacgacgg gcggcggagc
     1801 gggattccgg ggggtgtgcg agtatcgcga attcgtcggt ggggggccgg gcgggactcc
     1861 tgcctgcccg tgccgggcat acccctacac gggctggaaa accccactcg aatagtgcag
     1921 aattgtacag atttctgctg ccgtgtaggg ttttccccca caacacggca cagggacggg
     1981 ggcccctgac ggcattggcg cccggcatgg ttctggttac tttcccgtgg gcgtaaggcc
     2041 tgcccggcag gccgcataca cgaccaacca ccagattccg aagaccatgc tccgcaccct
     2101 gattcccgcc atcgccctcg tggcactcgc gttctccggg cgggccgtac agcccgagcc
     2161 ggttgccgag ccggacgggc acgcctggct tgtccaggat caccagttca tcggcgccac
     2221 gcgctgccgc acctgccacc ggaaggagga ggacggcgcc cagttcgaca agtgggccgc
     2281 gagcgcccat gccagcgcct acacgacgct tgcgtccgac caggccaagg ccatcgccgc
     2341 cgagaagggc atcgaagatc cccagaaggc cgccgagtgc ctggtctgcc acacgaccgg
     2401 ccacggtgtt ccggccgaga tgctgggtga caagtacgac atcgccgagg gcgtgacctg
     2461 cgaggcctgc cacggcgcgg gcggcgacta ctacaagaag gccactatgg aaggcatcgc
     2521 cgccggcgat atcgagggcg cctccgtggg cctcgtaatg ccgacggccg agacctgcat
     2581 cacgtgccac aacgagaaga gcccgacctt caaggcgttc aactatgaag agcaggtcgc
     2641 gaagatcgcc catccgaatc cgaacaactg atccggatcg gcgacggggc gcggcgcggg
     2701 cattcgcgct cgccgcgctc ctggtcctgg cggccgtgcc ggagcgctcc tcggcgcagt
     2761 tccggtacga cggacaggtt tacgcgatgg cctatgcgtg ggaagcgcct gccgacgaaa
     2821 gcggcaggcg cttcgacacg tacgagggcc ttcgtctccg ggtgcgtccg ggtgatgccc
     2881 tggacgtacg gacccatgtg ctgttcgccc ggcgttccag aatggacgcg gcggggagcc
     2941 tgtcgtacga gtgggaggag cgggtgtacc agctgtacgc cgacgctcgg ctctcgggcc
     3001 gcgagctgac cggccgcctc ggccgccagt tcgtgtaccg cgggacgatc aacggcacct
     3061 ttgacgcgct ggccctccgc tacaggcccc gggcggactg ggatgtccag gccctggcgg
     3121 gcttcgccgc gccgtacgcg cgcgacctgg acttcactcc cgcgtccgac gacgagaacc
     3181 tctgggacga gcggggctca ctggggcttt tcgtctcgaa gacgttcgga cggcgcatcc
     3241 gggccaacgc ctcctggttc cagcgccacc gcagcggcga catcgcctgg agcctggcgg
     3301 gcgtaggttt ctccgcggga tacggccacg gccgcgtcgt gcaggcggag gtcgaataca
     3361 ataccgagca ggagagcatc cagcggatgc gggtgcgcga ctggggtgag atcggccgct
     3421 ggatcctctc gatcgaggcg tccacgcagc gtccccgcat cttcgaggat tccttcttct
     3481 cgcggttctc ggtcaccggg tacgggcagg tccggctcgg cctgtcgtac gatctcgggt
     3541 ccggacgggc cggatacgag ttcgtcggga cggacttcga gggcgaccag cgctccggcg
     3601 agcacatcct gacgtacggc ctcgaccagg ggcgcttctc gggcatgctc ggcttcgtgt
     3661 accaggccgg gtacgggggc gagcgcatct cgccctacgt ggacgcgcgc tacgagctgc
     3721 tcgacggcgt gacgctgcgc ggcaacttca cctacctcga gtacgagcgc cggacgatca
     3781 gcctggacga ggacgcgacc gcctggacgg cgggtgtcct ggtccgcccc gtttcgcgct
     3841 ggtggctgca gctggacgtg cagcaggccg ccaacacctt ctacgaccag gatacgcgcc
     3901 tgctggcccg tgccggcgtc tcgttctgac cgtaaccgcc gacagcatac catgaaagta
     3961 cttgccgccg ttctgttcgt gccggcgacg ttcttctggc agatggccgt catgccgatg
     4021 gcgcttccgg gctccatcga cggcggggtg cgtttctcgc accggctgca catggaggac
     4081 gcgggcacgg cctgcgcgga ctgccacgag ccggccgccg agtcgatgac gggcgccgac
     4141 aacctgctcc cggatcacga cgtgtgctcc gcctgccacg aggtcgagga gccggacgag
     4201 tgcggcacct gccacaccga cgcggacgac ccggagggcc tcgtttacat caccgcctac
     4261 tcgacgctct ttccccacgc gacgcacctc gcgggcggct tcgagtgcgc agactgccac
     4321 gccggcatcg aggccgcgga gcccgatgac gacgtcgagc tgcccggcat ggcgcggtgc
     4381 gtggactgcc acaatgaaga ggtggcgtcc gtcgagtgcc ggacctgcca cgaggcgggc
     4441 gaggacctga tgccggccac ccacgtggtc ggattcgccc ggacccacgg cccgcaggcg
     4501 gcccagaacg cgatctcggc gcacggcgac ctgacgtgcc agacgtgcca tgacgacgtg
     4561 ttctgccagg actgccacga gggcgagaac ctggatcgac tctcgcaccc gctcaactac
     4621 gagttcaccc atgcgctcga cgtgcagtcc ggggcggtga actgcacgac gtgtcacacg
     4681 gagaccgcgt tctgcgtcga ctgccacgcg gagaaccaac tcatgccgca gacccacacc
     4741 gccggctggg tgaacgcgct cgacggcggc aggcaccgga tcgaggcgct caacgacctc
     4801 gatacctgca tcgcgtgcca cgaggacgat gccgagatca cgtgcggagg atgccatgtc
     4861 gactagagca ttcggaatcg cggcccgcct gtcggcggcg ctcgggacgg tcctgttcgt
     4921 ggcgttcgcg gtcgggtgcg cggacctcgc cgacgagccg gaggactatt accatccggc
     4981 ggagtggatg gtgtcggcgt ccgccgattt ccacggggcg gcggtgctcg ccagcccggg
     5041 aaaggccgag tcctgccaga cgtgccacgg cgaaacgtac gggggcggca cgtcgggcgt
     5101 cgcctgtacc acgtgccacg cggcctatcc gcacgaagcc gcgttcggcg gcagtcccag
     5161 gcccacgcac gcggcgttca tgcgcgagca ggcggggtgg gacctgtcca tgtgccagtc
     5221 gtgccatgga gcggcatatg acggcgacgg cgtggcctcc aagacctgcc tgacctgcca
     5281 cgacggggac ggtgggccgg aggcctgcaa cacctgccac ggcgacgacg cgaccggcgt
     5341 gccgccgccc gatctcctcg gcaacgaggc ctcgacgttc cgcggcgtgg gcgcgcacga
     5401 ggtgcacgcc gcgctcggca ccgggtccga ggcctgcctg tcgtgccacg cggtgcccgc
     5461 gtcctacgga gcggccggtc acatcgatga cgacccggcg cgtgccgaga tcgtgttcgg
     5521 gggcctggcg gtgcatgcgg gggccgagcc atcctacgac gcgtcggccg gcacctgcgg
     5581 gtccacgtac tgccacggcg gcttctcctt cccgaaggag ggcagcccgt acgcgttcgc
     5641 gtacacgggc gacttcatca cggggaacgg cccggaggtc gagtggacgg ggggcgaggc
     5701 cgaggcgacc tgcggatcct gccacggcct cccgccgacc ggccacatca acaacgccgc
     5761 ctgcaccaac tgccacaaca ccttcccggc aaaccacatc gacgggaaga tcaacgtgtt
     5821 cggctcgtcc cgctgaccgg cgggaagttc gcccggtcac cactgatcgc agccggcccg
     5881 gggccggcgc gaggtccggc acggccgtag gggttccccc tgcgcgccgt gcttccggtt
     5941 tcctgcggcc cgtgtcggtc agggactacg tccggcactg gccctgtcct acggcgaacg
     6001 tcgacttccg ggagcatctt tcggccgcgt gagagcccgt ccacggccgg gctttcacgc
     6061 cggaaaacag cgcagcacga ccatgacgac gcctcgcgga ttgctcatcg tgttggccgg
     6121 cttcctgctg atgccggtca cggccgccgt cgcccaggaa ggggacgacg cggagtgcat
     6181 ggcgtgccac gacgaccgga gcctgatcgt ggagcgggac ggccggcgcg tgtcgatgca
     6241 cgtgaactac caggcgttcg cccagtcggt gcatgcgcgg gagggctgca tctcgtgcca
     6301 tgccgacgtg gacgtcgacg acctgcccca tgccgaggac ctggatcggg tcgactgctc
     6361 catgtgccac gacgacgagg tggagcagtt caccgccagc ctgcacgggc aggccgccca
     6421 ttccaaccgg tttctcgcgc cgtcgtgcac gacctgccac ggcaagcacg acatcctctc
     6481 ttcgaagaac agggccgcgc ggacctacgt gaccaacatt ccctcgctgt gcggagagtg
     6541 ccacaaggac ggcacgcgcg tgagcgagct ccgtgcgata agccagcggc acatcctcga
     6601 ggactacgcg gagtccatcc acggggacgg cctgttccgg cgcggcctca tcgtgacggc
     6661 cgtctgcacg tcctgccact tcgcgcacaa catcctgccg cacgagaacc cgaactcgtc
     6721 gatcaaccag gcgaacatcg cctcgacgtg ccagcagtgc cacgccaaga tcgaggaggt
     6781 gcacgtcaag atcatcgacg gagagctgtg ggagaagcgg ccgcacgagg tcccgatctg
     6841 cgtggattgc caccagccgc accgggtccg gcgggtggac tacacggcga gcttcccgga
     6901 ccgccggtgc ctggactgcc actcgaatcc ggacctctcg gtcacggagg cggacggctc
     6961 gatgcgcccc cttttcgtgg atccggacga cctgcttcac tcgtcgcacc agaaccagtc
     7021 gtgcatcagc tgccacacgt cggtgaccga gagccgggat cccgtctgcc tggactccgg
     7081 tccggtggac tgcgccatgt gccacgcgga gcaggtggac cagttcgcaa tcagcacgca
     7141 cgggcagaag gtggcctccg gagacgagcg ggcgccctac tgcaccacgt gccacgggga
     7201 ccatcgcgtg ctcgcgccgg acaacccgga atccccggtc tttccgcgga acctgccgga
     7261 cctgtgcgcg gggtgtcacg aaaaccgggt cgagcccatc ctgacgaacg cccgggacgg
     7321 agacaacatc ctcacggact actccggcag catccatggg aaggggctgc tccagagcgg
     7381 cctcctggtg acggccacgt gcgtggactg tcacacggcg cacatggaac tgccgatcag
     7441 cgacacgctc tcgacggttc atccgtccaa cgtcgcctcg acctgcgccc ggtgccacga
     7501 cggcatcatg gagcagttca accggtcggt ccactcgccg ctcgcgaacg acacggacca
     7561 gaagctgccg gcctgcaacg actgtcaccg ctcgcacacg atccgacgga ccgacgccgg
     7621 ggacttccgc caggacatgc tgaaccagtg cggggactgc cacgcggaag tcaccgagac
     7681 gtacttcgac accttccacg gcaaggtgtc gcagctcggc tccgaggtca ccgccaagtg
     7741 ctccgattgc cacggggccc acaacatcct gccgccgacc gacccggaat cgatgctcag
     7801 ccggcagaac gtggtcgaga cgtgcggaaa gtgccacccg agctccaacc ggcagttcgt
     7861 gggctacctg acccacgcca cgcaccatga ccgggacaag tatccctacc tgtactacac
     7921 gttctggttc atgacgtcgc tgctcgtggg cgtc
//
LOCUS       DBNHQZ010000431         3612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_668768, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000431 DBNHQZ010000000
VERSION     DBNHQZ010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_668768"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="AC16YU_11635"
     CDS             <1..176
                     /locus_tag="AC16YU_11635"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6065922.1"
                     /translation="PASIALSRELKRHGWRFFGPTTAYAFMQAMGLVNDHQEGCCCRA
                     PALAARQDFLRPS"
     gene            366..971
                     /locus_tag="AC16YU_11640"
     CDS             366..971
                     /locus_tag="AC16YU_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR6065923.1"
                     /translation="MKTRFLSLFLPVLAAVMMLGCTESPVVAQSDDSEIAAKRGTAPG
                     QQDMTILDLVLYLSGQEEGADFTILRDVVVGISQNTDIDLVAALSSEDDQVTVFAPND
                     AAFVALLGDLGASSLEDVVALLGWDGLASVVLYHVTPGRRMAGTVLMQKQLPTLNGAV
                     IEKEKGGAVLNGSVTIIAPDAGIASNGIVHVIDRVLLPPTE"
     gene            complement(1072..2301)
                     /locus_tag="AC16YU_11645"
     CDS             complement(1072..2301)
                     /locus_tag="AC16YU_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR6065924.1"
                     /translation="MSWTGVLVFVATYALISARRLSWLGFDRPAGALLGAVACVVLGV
                     LGPSEALGAVNGPTILLLFGVMGMGAFLAVDGFFDGLERAVTRRAATPARLLALVVWG
                     AGLLSAFITNDAVCVLAAPLVVRLVRRHELPPLPFLLALATAANTGSVATLVGNPQNM
                     LCAVLGDLSFRTHLAVAGPLAVVALAVNHGVLWLAFRRRLPRGPLANGEAAGRISGRQ
                     ALTLAVILGTAVAYTAGADLAWAATAGFAALMLMHRRDTRDLWARVDWSLLLFFAGLF
                     VVVEGLMRSGAPDLVFDRFPLAGSGTEGPADWLRLGGIFLAGSNAVSNVPFILIVRDQ
                     MATLARPELAWELLAVASTFAGNLTLLGSVANIIVAESARDIGGIGFFDYLKIGFPLA
                     VATTVLGTLWLLAFFPA"
     gene            3046..>3612
                     /locus_tag="AC16YU_11650"
     CDS             3046..>3612
                     /locus_tag="AC16YU_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HIR6065925.1"
                     /translation="MLLIVLLSGPATAQPVRNGDDVVWARDVAGAALTLDGVLDEAAW
                     ASAEAIPLVWNQEHGAPGDGQKIEYNPLIADPLDGNDGTLYILRDGNTLWMGLEANDK
                     SIGGSRSLWRFDGIVMAMLDRSELALIDYVGPNSFVMNPTEWIYGWFNPADTLAGGLP
                     IPDGPPRFFGNYGVGFNDPYDAERTPEKE"
BASE COUNT          625 a         1233 c         1206 g          548 t
ORIGIN      
        1 cgcccgcctc gatcgcgctc tcgagggagc tcaagcgcca cgggtggcgc ttcttcggac
       61 cgaccacggc ctatgccttc atgcaggcga tgggactcgt gaacgaccac caggaggggt
      121 gctgctgccg cgcgccggcc ctcgccgcgc gccaggactt cctgcgcccg tcgtagcccc
      181 cgtcggggcg aggcgcatcc gcggatgtcg atcggatctg gcggatgtga aacgccgaaa
      241 tcgtctattc gaaacgattc tacgggccaa aacagtgata atgtattttt ttgttcaaac
      301 aagttggacg aattggacga tttgttgtat tatgggtcac ccaacaacaa agcccggctc
      361 gaaccatgaa aacccgtttc ctctccctct tccttcccgt cctggccgcc gtcatgatgc
      421 tcggctgcac ggagtccccg gtcgtcgccc agtccgacga ctcggagatc gccgccaagc
      481 gcgggaccgc ccccggccag caggacatga ccatcctgga cctggtcctg tacctgtcgg
      541 gacaggagga gggcgccgac ttcaccattc ttcgtgacgt ggtcgtcggc atcagccaga
      601 acaccgacat cgacctcgtc gccgctctct ccagcgagga cgaccaggtc actgtcttcg
      661 ccccgaacga cgcggccttc gtcgcgctgc tcggcgatct cggagcctcg agcctcgagg
      721 acgtggtcgc gctcctcggc tgggacggcc tggcctcggt ggtgctctac cacgtgacgc
      781 ccggccgccg catggccggc acggtgctga tgcagaagca gctgccgacg ctcaacggcg
      841 ccgtgatcga gaaggagaag ggcggcgccg tgctcaacgg atccgtcacc atcatcgcgc
      901 ccgacgcggg gatcgcctcg aacggcatcg tccacgtgat cgaccgcgtt ctcctgcccc
      961 cgaccgagta attccaccgg tcgatgcacg ccgcggacgg ccgtaaccgc ccgccgctcg
     1021 aaggcctcgg cgcgatcgcg tcggggcctt cggcgtttcg acggcgggcg gtcaggccgg
     1081 gaagaacgcg aggagccaga gggtgccgag gaccgtggtc gcgaccgcca gcgggaagcc
     1141 gatcttcagg tagtcgaaaa agccgattcc accgatatcg cgcgcactct cggcgacgat
     1201 gatgttggcc accgagccga ggagcgtcag gtttcccgcg aaggtggacg cgacggcgag
     1261 cagctcccag gcgagctcgg gacgggccag ggtcgccatc tggtcccgga cgatcaggat
     1321 gaaggggacg ttggacaccg cgttcgagcc agccaggaaa atcccgccga gcctgagcca
     1381 gtcggccggc ccttccgtgc ccgagccggc aagcgggaag cggtcgaaca ccaggtccgg
     1441 cgcaccgctc cgcatcaggc cctctaccac gacgaagagg ccggcgaaga agaggagcag
     1501 cgaccagtcg acccgggccc agagatcgcg cgtgtcccgg cggtgcatga gcatgagcgc
     1561 cgcgaatccg gccgtcgccg cccaggccag gtcggcgccc gcggtgtagg ccacggccgt
     1621 acccaggatg acggccagcg tcagtgcctg tcgtccggag atccggccgg ccgcctcgcc
     1681 gttcgcgagg ggcccgcggg gaagccggcg gcggaaggcg agccacagta ccccgtggtt
     1741 gacggcaagg gcgacgacgg cgaggggtcc cgccaccgca aggtgggtcc ggaaggagag
     1801 gtcgccgagg acagcgcaga gcatgttctg cgggtttccg acgagcgtcg ccacgctgcc
     1861 ggtgttcgcc gccgtcgcga gggcgaggag gaacggcagg ggcggcagtt catggcgacg
     1921 cacgaggcgc accacgaggg gcgccgcgag gacgcacacc gcgtcgttcg tgatgaaggc
     1981 gctgaggagc cccgctcccc atacgacgag cgcgagcagc cgggcgggcg tcgcggcccg
     2041 gcgcgtgacg gcccgctcca ggccgtcgaa gaacccgtcc acggccagga acgcccccat
     2101 gcccatcacg ccgaagagaa ggaggatcgt ggggccgttc accgcgccga gcgcctcgga
     2161 ggggccgagg actccgagca ccacgcacgc gaccgcgccc aggagagcgc ccgccggccg
     2221 gtcgaagccg agccacgaca gccgccgcgc cgaaatcagc gcgtaggtcg cgacgaagac
     2281 gaggacaccg gtccagctca tgcgggaagc agtccgacgg acggatggag gggagccggc
     2341 ccccatcccg tgtttcggac tggtttcgaa tgcgaaaaac gacgttcccg atgccgcaaa
     2401 ttatgtttcg atgttgtttc gcgatagtgg ctccaagggc tttgattctc cgggcgatgc
     2461 cgataccatg cacccatgac gcatcacgga cgcccccccg gatgacttga gtccgtgttc
     2521 gtgaaccgtc cggttcgcgc gctcgtgagc tgagcccggc ctcgattccg gccgggctct
     2581 tttgtttgtc cgcggatcgc ctgccgggcc acaccgaatg atcgaacgac cgcggggcgg
     2641 agcgggccgt cgaggtccgc ggggcacgcc cggggggggc gcttacgcac attccagagg
     2701 cgcacgcccg tagtgccgtg accctcgcgc cattccgggg tcgcactgcg cggatttcgg
     2761 accggtctgg gcacaattcc cttccctttc tcccgtcggg gctgtatcta aacgatatcg
     2821 gcgtggtcgt ccggtggcac cctggtacgg attgcgccga atcccacgac atcgaaccca
     2881 cctgcgcttt cgcttcgagc ggcctgcgat tctctcggcg acggttctgc ccccagggcc
     2941 gtggccggag ggtgatcacg aggctcgtcg cgaaggcatc aagtcctgca tatccgatcc
     3001 gaggtgacac caatgcagaa atcccttaca acggcaagcg cttccatgct gctcatcgtc
     3061 ctcctgtcgg gccccgccac cgcgcagccc gtgcgcaacg gggacgacgt ggtatgggcg
     3121 cgcgacgtgg ccggcgccgc gctcacgctc gacggcgtcc tcgacgaagc cgcctgggcc
     3181 tcggccgagg cgatcccgct ggtctggaac caggagcacg gcgctccggg cgacggccag
     3241 aagatcgagt acaacccgct catcgccgac ccgctcgacg ggaacgacgg cacgctctac
     3301 atccttcgcg acggaaacac gctctggatg ggtctcgagg ccaacgacaa gtccatcggc
     3361 ggcagccgct ccctgtggag attcgacggc atcgtcatgg cgatgctcga ccgctcggaa
     3421 ctcgccctga tcgactacgt cggccccaac agcttcgtga tgaatccgac cgagtggatc
     3481 tacggatggt tcaaccccgc cgacacgctg gcgggcggat tgcctattcc tgatggtcct
     3541 ccgcggttct ttggcaatta cggcgtcggt ttcaacgatc cgtacgacgc cgagcgcacg
     3601 ccggagaagg aa
//
LOCUS       DBNHQZ010000432         3208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2159732, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000432 DBNHQZ010000000
VERSION     DBNHQZ010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2159732"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="AC16YU_11655"
     CDS             <1..527
                     /locus_tag="AC16YU_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6065926.1"
                     /translation="VHEMGLHDTVAALVTVATPHHGSSLADVVLEQPERVREWMTDAA
                     RWVSDAAMQGTPADFRRAVADLTPEYVTGTFNPAVPDHPSVRYWSWSARAGRGTDTPI
                     NPLLRPLNAMLYAREGPNDGFVSVESAVWGEHLGILEADHAQQIGVGKSIGSRFDSSR
                     FYAEVAERLGREGF"
     gene            complement(541..1011)
                     /locus_tag="AC16YU_11660"
     CDS             complement(541..1011)
                     /locus_tag="AC16YU_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065927.1"
                     /translation="MRPVLVGLFLLALLPACDSQKETEQFENEASLTPVGFTRTDEYG
                     DVLSGDEDDWRMAPRFLGKVRVDPAYPNPVGTGIVTIPVSVLEFDGVGGGLVLRARSS
                     SGRLIILDDIPDAGSPGAYVFQFSSVLLARTGLVRLFVMDLRGELISYGDLMIE"
     gene            1097..1906
                     /locus_tag="AC16YU_11665"
     CDS             1097..1906
                     /locus_tag="AC16YU_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065928.1"
                     /translation="MRALDVENLTVRLAGRPVLHEVGFGVDRGSWLGVLGPNGSGKTT
                     LLRAIGGLIPFEGAIRLDGRPLDEWPDRERARRLAFVRQSLGIAFDFSVQDVVLLGRA
                     PHKGWLTPFDRNDRRLVRDALHHVDLEGFEDRSMFALSGGEQRRVLLAQALVQQADLL
                     LLDEPTTHLDVHHQYEFLDHVRRLVDDGATAIGVFHDLELAARYTDRVLVLDEGHVRA
                     NGPAREVLSTELIARVFLMEVESRGAGDGDLGLRYRAPIPRPERNGGSPEA"
     gene            1931..2482
                     /locus_tag="AC16YU_11670"
     CDS             1931..2482
                     /locus_tag="AC16YU_11670"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR6065929.1"
                     /translation="MKIYTRTGDAGDTALFGGGRVPKDHPRIRAYGTVDETNAFLGQA
                     RSHVRGNGSFSVLDDMLERVQNELFVVGADLATPTESKASVPRVGPAHIEALERDIDR
                     LDADLPELRRFILPGGHPCAATLHAARTVCRRAEREIVTAFEDPGVSRDAAVYLNRLS
                     DFLFVAARWANRAAGLEDASWTP"
     gene            2494..>3208
                     /locus_tag="AC16YU_11675"
     CDS             2494..>3208
                     /locus_tag="AC16YU_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065930.1"
                     /translation="MAAPPAEPTRGRRLAFTAAMLALPVLFFVALEGGLRMAGYGEDL
                     PLFVPVQGAPEWLVQNPQVARRYFSRQARVPTGLGDAFRARKDSAAYRIFVLGGSSAA
                     GYPYYFGGAFSRMLQQRLQQTFPGRRIEVVNTGLAAINSYALVDFSREILGQAPDAIL
                     VYAGHNEYYGALGVASSESIGRSRALVRAYLALQRFRTVQLLRAGLARLAALGAGESG
                     TPPGRTLMERMVGEQRIPAG"
BASE COUNT          446 a         1193 c         1111 g          458 t
ORIGIN      
        1 tggtccacga gatgggatta cacgacaccg tggccgcgct cgtgacggtg gcgaccccgc
       61 accacggatc ctccctcgcc gacgtcgtgc tcgagcagcc cgagcgggtc cgcgagtgga
      121 tgaccgacgc ggcgcgctgg gtgtccgacg ccgcgatgca ggggacgccg gccgacttcc
      181 gccgcgcggt ggccgacctg acgccggagt acgtgaccgg aaccttcaac cccgccgtcc
      241 ccgaccaccc gtccgtgcgc tactggtcct ggtcggcccg cgccgggcgc ggcaccgaca
      301 cgcccatcaa cccgctcctg cggccgctga acgccatgct ctacgcgcgc gaggggccga
      361 acgacggatt cgtctcggtc gagagcgccg tgtggggcga gcatctcggg atcctggagg
      421 ccgaccatgc ccagcagatc ggggtcggca agtcgatcgg ctcgcgcttc gattcgagcc
      481 ggttctacgc ggaggtagcg gagcggctcg ggcgcgaggg gttctgacgg ctcggcgggc
      541 tcactcgatc atcaggtcgc cgtaggagat gagctccccc cgtaggtcca tcacgaacag
      601 ccgcaccagg cccgttcgcg caagcagcac ggacgagaac tggaacacgt aggcgccggg
      661 gctcccggcg tccgggatgt cgtcgaggat tatcaggcgg ccggagctgc tccgcgcccg
      721 gaggaccagg ccgccgccca ccccgtcgaa ctcgaggacc gagaccggga tcgtcacgat
      781 cccggtgccg accgggttcg ggtaggccgg atcgacccgg accttgccca ggaagcgcgg
      841 cgccatgcgc cagtcgtcct cgtccccgga gagcacgtcc ccgtactcgt cggtccgcgt
      901 gaaaccgacg ggcgtgaggc tcgcctcgtt ctcgaactgc tcggtctcct tctggctgtc
      961 gcaggccgga aggagcgcga gcaggaacag gccgaccagt acggggcgca tgccggtcat
     1021 catggggttc gctcgaaatc tcgtccggtc gagaacccgg ccgccgcccg cctcgttccc
     1081 cgcgaaaccg gagaccatgc gcgcgctcga cgtcgagaac ctgaccgtac ggctggccgg
     1141 acgcccggtg ctgcacgagg tcggcttcgg ggtcgaccgc ggctcctggc tcggcgtcct
     1201 ggggccgaac ggcagcggca agacgaccct cctgcgcgcg atcggcgggc tcataccctt
     1261 cgagggggcg atccggctcg acggccgccc gctcgacgaa tggccggacc gcgagcgcgc
     1321 ccgccgcctc gccttcgtgc ggcagtcgct cggcatcgcg ttcgactttt cggtacagga
     1381 cgtggtcctc ctgggccggg cgccgcacaa gggctggctc acgcccttcg accggaacga
     1441 ccgccgcctg gtgcgcgacg cgctccacca tgtggacctg gaagggttcg aggaccgctc
     1501 gatgttcgcg ctctcgggcg gcgagcagcg ccgcgtactg ctggcgcagg cgctcgtgca
     1561 gcaggcggat ctcctgctgc tcgacgagcc cacgacgcac ctggacgtac accaccagta
     1621 cgaattcctc gatcacgtcc gccggctcgt ggacgacggg gcgacggcga tcggcgtgtt
     1681 ccacgacctg gagctcgcgg cgcgctacac cgaccgcgtg ctggttctcg acgagggcca
     1741 cgtgcgcgcc aacggcccgg cccgggaggt gctctcgacc gagctcatcg cgcgcgtgtt
     1801 cctcatggag gtcgaatcgc gcggcgccgg cgacggcgac ctgggcctgc gctaccgcgc
     1861 tcccattccc cgcccggagc gaaacggagg gagtccggag gcgtagtcag gccccacatt
     1921 cgcgacgccc atgaagatct acacccgcac cggcgacgcc ggcgacaccg cgctcttcgg
     1981 cggcggccgg gtccccaagg accacccccg catccgggcg tacggaacgg ttgacgagac
     2041 caatgccttc ctgggccagg cgcggtcgca cgtacggggg aacggatcgt tctcagtgct
     2101 cgacgacatg ctggaacggg tccagaacga gctcttcgtg gtcggcgccg acctcgcgac
     2161 gcccacggaa tcgaaggcgt ccgtgccccg ggttggcccg gcccacatcg aggcgctgga
     2221 gcgggacatc gaccgcctgg acgccgacct ccccgagctt cgccgcttca tcctgccggg
     2281 cgggcatccg tgcgccgcga cgctgcacgc cgcgcgcacc gtctgccgga gggccgagcg
     2341 cgagatcgtc accgcattcg aggacccggg cgtgagccgc gacgcggccg tctacctgaa
     2401 ccgcctctcg gatttcctgt tcgtcgccgc ccggtgggcg aaccgggccg ccggcctgga
     2461 agacgcctcc tggacaccct gacctccgga tccgtggccg cccctcccgc agagccgacc
     2521 cgcggccgcc gcctggcctt cacggccgcg atgctggccc tgccggtcct gttcttcgtc
     2581 gcgctcgagg gcgggctgcg catggccggg tacggcgagg acctgcccct cttcgtcccc
     2641 gtacaggggg ccccggagtg gctcgtgcag aatccgcagg tcgcccgccg ctacttcagc
     2701 cggcaggctc gcgtgcccac ggggctcggc gacgccttcc gtgcccggaa ggactcggcc
     2761 gcgtaccgca tattcgtgct cggcggatcg agcgccgccg ggtaccccta ctacttcggg
     2821 ggcgccttct cccggatgct tcagcagcgg ctgcagcaga cgttccccgg acgccgcatc
     2881 gaggtggtca acacggggct cgcggccatc aactcgtacg ccctggtcga cttctcgcgc
     2941 gagatcctgg ggcaggcacc cgacgcgatc cttgtctacg ccgggcacaa cgagtactac
     3001 ggagcgctcg gcgtcgcctc gtccgagagc atcggccggt cccgggcgct cgtccgggcg
     3061 tacctggccc tgcagcggtt ccgcacggtg cagctcctgc gcgccggcct cgcgcgcctc
     3121 gcggccctcg gcgcgggcga gtcgggcacg ccccccggcc gcacgctcat ggagcggatg
     3181 gtcggcgagc agcgcatccc ggcgggct
//
LOCUS       DBNHQZ010000433         5345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_128310, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000433 DBNHQZ010000000
VERSION     DBNHQZ010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_128310"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..2058
                     /gene="mutL"
                     /locus_tag="AC16YU_11680"
     CDS             199..2058
                     /gene="mutL"
                     /locus_tag="AC16YU_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR6065931.1"
                     /translation="MNASTTAIDSGIIHVMPDVLANKIAAGEVIQRPGSAVKELVENA
                     LDAGARRIEVVVKDAGRTLIQVVDDGCGMSPLDARTAFERHATSKIRSIEDLERIRTL
                     GFRGEALASLSAVSRVELRTRRLADAQGTCVRVEGGEDLRSEPCAAPVGTSVSARQLF
                     FNVPARRAFLKTDATELRHVLDAVQYAALAHPEVAFVLVHDDTELLRVESEAGPDALA
                     SRVRGLSGHNPGRDLVPVDEETSYLRIHGLAGRPAVRRRARGEQFLFVNGRPVRNRYL
                     EHAVFSAYRGLVPEGSYPWFVLFLDIDPRHVDVNVHPAKAEVKFDDERGVYGMLRAVV
                     SRALSEWEGTPAVDAGLRDLLGGPAGQVSEPPKERAQGGGSRTDGWVGRAETASPGAA
                     SELLYGPSTGDARSAPGGLPAAGPSADEADDPEGTGPEDVRLWQLQDTYVVTPIRSGL
                     MVVDQYAAHERILYERALQALSDGFGLSQQLLFPRTVELSAADFALIGDLLADLRSLG
                     FDLDRLSGRTVLIRGVPADIRTGDERTVLEEVLEQFRDNARIERLGRRENQARSIARR
                     SAIRAGTRLDELEMRALIDQLFQCAQPYAGPDGRPTLVRIPVDELDRRFGRRR"
     gene            2070..3458
                     /gene="tilS"
                     /locus_tag="AC16YU_11685"
     CDS             2070..3458
                     /gene="tilS"
                     /locus_tag="AC16YU_11685"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6065932.1"
                     /translation="MSSRLRSIEETVQRQLDDHGLTDRKRPLVVAVSGGIDSVVVAHA
                     VHALGYRVVLAHVHHGLRGEEADRDAQLVEEMAREWNCPVHVVEIGDDLNEPSSGESM
                     QDRARRLRYAGLEHVAREVGSEVIITGHHRDDQAETVLLQLLRGTGPEGLAGMPAVRF
                     LSTDSPVRLLRPLLSQSRDRIRAYAETAGLAWREDASNESDVYLRNAVRRRVLPLLDD
                     VAGGESADRIARAAELLRQYLDSDLDALTEALLRGACRSLPGGGVLLDDRVLEPLPEV
                     WKGRLILSALESHLPAATRSRTTVDRALELLLRQTGRSVPVEGGAMWRERGGLALVPE
                     RPLPPPASLAVAALVEWDGTLFRLDPVSRPDTEDGRDGVILDADRLPPTLTIRAWSAG
                     DRLQPRGMSGSRKVKDLLVDARVAAHARARSPVVATGVDVVWVVGIRADRRFLADSGT
                     TNRVRLSASYSA"
     gene            3570..4136
                     /gene="hpt"
                     /locus_tag="AC16YU_11690"
     CDS             3570..4136
                     /gene="hpt"
                     /locus_tag="AC16YU_11690"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR6065933.1"
                     /translation="MSEVGRSIEINGERFRVYLDRETICRRVRDLAEAISRDYEGRIP
                     IFIGVLNGAFIFLADLMRHMTIECEVDFLKLSSYGAARITSGQVTELKRIDADIEGRH
                     VIVVEDIVDTGLSMDWIMEHLAEHRPASLATAVLLHKPDASRTRVSLDYVGFRIENKF
                     VVGYGLDYAQVGRGLPDLYMLDPAGRQA"
     gene            complement(4194..>5345)
                     /locus_tag="AC16YU_11695"
     CDS             complement(4194..>5345)
                     /locus_tag="AC16YU_11695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039904.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR6065934.1"
                     /translation="FPGAEHSRFGHALGAMALMHDTLASLAEKGTPIAAGEHTAACAA
                     ALLHDIGHGPFSHSLEHELIRGFRHEAMSRVLLEHLDQRFGGRLRDTIAIFDGTYPRP
                     FFHALVSSQLDMDRLDYLRRDSHYTGVVEGRVGVERIIKTLRVVEQDGVERMAIEAKG
                     FHAVENFLFARRLMYWQVYLHKAVLAGDHVLRAILRRVRHLVAGGEPEAVSGGAPDFM
                     FFLRNDVDAESLGEPEVRDRYCALDDADMLFSIKHWMSSTDPVLSDLATRFIHRRLPA
                     VTYFPAPVEPAVLDAWRGAVAHWLVERGMSSGGRAAADAEYYVAAGTARHSAYDTSDT
                     GIAILARDGSLADMGSAAESAPIAALGDGEEKHFVCHPKEVRSVYPPAA"
BASE COUNT          771 a         1813 c         1948 g          813 t
ORIGIN      
        1 catcgccgat tctcgtacga gccgcgcttc tacaacccgg agcgggagga gaagctcaag
       61 cggcggatcc ggatccggag ccatgcgcgc cgccggaatc cggcgggggt catctatttc
      121 gtgctgctcc tggcgatggt ggtgtacatc tacctgaagc tgggctgaaa gggcccggga
      181 cgaaggggga acggaagcgt gaacgcatcg acgacggcca tcgactccgg catcatccac
      241 gtgatgcccg acgtcctcgc gaacaagatc gcggcgggcg aggtcatcca gcggccgggg
      301 tcggcggtga aggagctcgt cgagaacgcg ctcgacgccg gcgcgcgccg catcgaggtc
      361 gtcgtgaagg atgcgggccg aacgctgatc caggtcgtgg acgatggatg cggcatgtcg
      421 ccgctcgatg ctcggaccgc tttcgagcgg cacgcgacgt ccaagatccg gtcgatcgag
      481 gacctggagc gcatccgcac gctgggtttc cgcggcgagg cgctggcgtc gctctccgcg
      541 gtctcccgcg tggagcttcg gacccgccgg ctggccgacg cacaggggac ctgcgtgcgc
      601 gtcgagggcg gcgaggacct gcggtcggag ccctgcgcgg cgccggtggg cacatcggtt
      661 tcggcgcgtc agctcttctt caacgttccc gcccggcggg ccttcctgaa gacggacgcc
      721 acggagcttc gccacgtcct ggatgccgtg cagtacgcgg ccctggccca tccggaggtc
      781 gccttcgtcc tggtccacga cgacacggag ctgctccggg tcgagtccga ggccggaccg
      841 gatgccctgg cgagccgggt gcgcggcctc tcggggcaca atcccggtcg ggacctcgtg
      901 cccgtcgacg aggaaacgag ctacctcagg atccacggat tggccgggcg ccccgcggtc
      961 cggcgccgcg cccgcggcga gcagttcctc ttcgtgaacg ggcgtccggt acggaaccgg
     1021 tacctggagc atgccgtgtt ctcggcgtac cggggcctcg tgcccgaggg gtcatatccc
     1081 tggttcgtcc ttttcctgga tatcgatccg cgccacgtgg acgtgaatgt ccatccggcc
     1141 aaggccgagg tgaagttcga cgacgaacgc ggcgtttacg gcatgctccg cgccgtggta
     1201 agccgggccc tgagtgaatg ggaagggact cccgcggtgg atgccggcct ccgcgacctg
     1261 ctcggagggc cggccgggca ggtgtccgag ccgccgaagg aacgcgctca ggggggtggt
     1321 tcacgtaccg acgggtgggt gggccgcgcg gagaccgcct cgccgggcgc agcgtccgag
     1381 ctgctctacg gcccgtcgac gggcgatgcg cgatcggccc cgggcggatt gccggccgcc
     1441 gggccctccg ccgacgaggc ggacgacccg gaggggacgg gtccggagga cgtccgcctg
     1501 tggcagctgc aggataccta cgtcgttacg cccatccgct cggggctcat ggtcgtcgac
     1561 cagtacgcgg cacacgagcg gatcctctac gagcgggccc tgcaggcgct ctcggacggt
     1621 ttcgggctgt cgcagcagct gctgtttccc cgcacggtcg agctttcggc cgccgatttc
     1681 gcgctcatcg gcgatctgct ggcggacctg cgctcgctgg gcttcgacct ggatcggctc
     1741 agcggccgca cggtgctcat ccgcggcgtg ccggcggaca tccggaccgg ggacgagcgt
     1801 acggtcctcg aggaggtgct cgagcagttc cgggataatg cccggatcga gcggctcgga
     1861 cggcgcgaga accaggcccg aagcatcgcg cggcggagcg cgatccgggc cggcacccgg
     1921 ctggacgagc tggagatgcg cgcactgatc gaccagctgt tccagtgcgc gcagccgtac
     1981 gccggccccg acggccggcc caccctcgtg cgcattcccg tcgatgagct cgatcgccgg
     2041 ttcggccggc gcaggtagga gtccttccga tgagcagccg actccgctcc atcgaggaaa
     2101 ccgtccagcg acagctcgac gaccacggcc tgaccgaccg caagcgaccc ttggtcgtgg
     2161 cggtcagcgg gggcatcgac tccgtggtcg tcgcccacgc cgtccacgct ttgggctatc
     2221 gcgtggtact ggcgcacgtc caccacgggc tgcggggcga ggaggcggac cgggacgcgc
     2281 agctcgtcga ggagatggcg cgcgagtgga actgcccggt ccacgtggtc gagatcgggg
     2341 acgatctgaa cgagccctcc tccggcgagt cgatgcagga cagggcccgc cggcttcggt
     2401 atgccgggct cgagcacgtt gcgcgcgagg tgggctccga ggtgatcatc acgggccacc
     2461 accgggacga ccaggccgag accgtgctcc tgcagctgct gcgcggcacg ggccccgagg
     2521 gcctcgcagg catgccggcc gtgcgcttcc tgtccacgga ctcgccggtc cgcctgctcc
     2581 gtcccctgct ctcgcagagc cgggaccgca tccgcgcgta cgccgaaacc gcgggactcg
     2641 cctggcggga ggacgcctcg aacgagagcg atgtttacct gcgcaacgcc gtgcgccggc
     2701 gcgttctgcc gctgctcgac gacgtggccg gaggagagtc tgccgaccgc atcgcgcggg
     2761 cggcggagct gcttcgccag tacctggact ccgacctgga cgccctgacc gaggccctgc
     2821 tccgcggtgc atgccggtcg ctgccgggcg gcggggtcct gctcgatgat cgcgtcctgg
     2881 agcccctgcc ggaggtctgg aagggacggc tcatcctgag cgccctggag tcgcacctgc
     2941 cggcggcgac ccgctcgcgg accacggtgg accgggccct ggagctgctt cttcgacaga
     3001 ccggccggtc cgtaccggtc gagggcggtg cgatgtggcg cgagcggggt ggactggccc
     3061 tggtgccgga gcggcccctt ccgccgcctg cttccctcgc cgtggccgca ctggtcgagt
     3121 gggacggcac cctgttccgc ctggatccgg tgtcccgccc ggacacggag gacggccgcg
     3181 acggggtgat cctggacgcc gaccggctgc cgcccaccct cacgatccgg gcctggtcgg
     3241 cgggcgaccg gctgcagccg cggggcatgt cgggatcgcg caaggtgaag gacctgctcg
     3301 tggacgcccg cgtggccgcc cacgcacgcg cacgttctcc ggtcgtggcc acgggggtcg
     3361 atgtcgtgtg ggtcgtggga atccgggcgg accgtcgctt tctggccgac tccgggacga
     3421 cgaaccgggt gcgcctgtcc gcctcgtatt cggcctgagc cccgactgcg atccgaaggg
     3481 cgagcgggat gccgcgccgg ccgggcgcga ttgcggacga aggcccgtat cttccggccc
     3541 ttcgcagcca aagctcacct caggtatccg tgtcggaagt cgggcggtcg atcgagatca
     3601 acggggagcg gttcagggtg tacctggacc gggagaccat ctgccggcgc gtgcgcgacc
     3661 ttgccgaggc gatcagccgc gattacgagg gcaggattcc catcttcatc ggcgtgctga
     3721 acggcgcatt catcttcctg gccgatctca tgcgccacat gacgatcgag tgcgaggtgg
     3781 acttcctgaa gctctcgtcc tacggcgccg cgcgcatcac gtcgggccag gtgaccgagc
     3841 tcaagcggat cgacgccgac atcgagggcc gtcacgtgat cgtggtcgag gacatcgtgg
     3901 acacggggct gtcgatggac tggatcatgg agcacctggc ggagcaccgt ccggcctccc
     3961 tggccacggc cgtgctgctg cacaagcccg acgcctcgcg cacccgcgtc tcgctcgatt
     4021 acgtgggctt ccgcatcgag aacaagttcg tcgtggggta cggcctggac tatgcgcagg
     4081 tggggagggg gcttccggac ctgtacatgc tcgacccggc cggtcgccag gcctagggcc
     4141 cacggcgcgg aagcgcccgg gacgggacgc tgagccccgg atccggcggc gtgtcaggcg
     4201 gccggagggt acacggagcg gacctccttc gggtgacaga cgaagtgctt ctcctcgccg
     4261 tcaccgagcg ccgcgatggg tgcggattcg gcggcggagc ccatgtcggc gagcgagccg
     4321 tcccgggcca ggatggcgat ccccgtgtcg gaggtgtcgt aggcggagtg ccgcgccgtg
     4381 ccggccgcga cgtagtactc ggcgtccgcg gcggcgcgac ccccgctgct catgccccgt
     4441 tccacgagcc aatgggccac ggctccgcgc cacgcgtcga gcaccgcagg ctcgacgggc
     4501 gcggggaagt aagtgaccgc gggaagcctc cgatggatga agcgggttgc gaggtccgag
     4561 aggaccgggt ccgtgctcga catccagtgc ttgatcgaga agagcatgtc cgcgtcgtcg
     4621 agcgcgcagt acctgtcgcg gacttccggc tctcccaggc tctccgcatc gacgtcgttg
     4681 cggagaaaga acatgaagtc gggcgccccg cccgagacgg cttccggctc gccgcccgcc
     4741 accaggtggc gaacccgccg gaggatggcc ctgagcacgt ggtctcccgc gagcacggcc
     4801 ttgtgcaggt acacctgcca gtacatcagg cgccgggcaa agaggaagtt ctcgaccgcg
     4861 tggaatcctt tcgcctcgat cgccatgcgc tcgacgccat cctgctcgac tacgcggagg
     4921 gtcttgatga tccgctccac accgacccgc ccctcgacga ctcccgtgta gtgggagtcg
     4981 cggcggaggt agtcgagccg gtccatgtcg agctggctcg agacgagggc atggaagaac
     5041 ggcctggggt aggtcccgtc aaagatcgcg atggtgtcac gcagccgccc cccgaaacgc
     5101 tggtccaggt gttccaggag cacgcggctc atcgcctcgt gccggaatcc acggatgagc
     5161 tcgtgctcga gcgagtggct gaacggaccg tggccgatgt cgtgcagcag ggcggccgcg
     5221 cacgccgcgg tgtgctcgcc cgccgcgatc ggggtgccct tctcggcgag gctggcgagc
     5281 gtgtcgtgca tgagggccat cgcgccgagg gcatggccga agcgcgagtg ctccgcaccc
     5341 ggaaa
//
LOCUS       DBNHQZ010000434         9896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2836621, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000434 DBNHQZ010000000
VERSION     DBNHQZ010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2836621"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="AC16YU_11700"
     CDS             <1..778
                     /locus_tag="AC16YU_11700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6065935.1"
                     /translation="ITEPDHGSDITTMNTTAVRDGDEWVVNGVKTFITNAGIASFYVV
                     VCQTDPEAGHRGQSMIVVPADAEGLGTADVGSKMGIRMIPTGELSFRDVRVPAENLVG
                     AEGKGFYQILEFFDESRVETAAQGLGTAQGAFDRALEYVKSREQFGKKIAQFQATQHK
                     LAEMATKIELARLVTYKAAWNFDQGRIDPKLTSMAKWYAGRTAVEVADEAIQLLGGYG
                     YIAEYEVERFYRDAKICEIYEGTKEIQKNTIAGAVIGKLK"
     gene            complement(787..1578)
                     /locus_tag="AC16YU_11705"
     CDS             complement(787..1578)
                     /locus_tag="AC16YU_11705"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR6065936.1"
                     /translation="MKRRDFITTTALAGIGLGAHSSSVPVAAGSSGPPEPRQILIAGG
                     GFGTAFIRYMAGLTGKERPRICYLPTASADSPYGSIRFFEHCAPLDVHPLVQESFIVS
                     SRMDQTWEEVLLSMDGIVVSGGNTLNQQVIWKAHGIDAVLREAWDRGIVLGGASAGSL
                     CWFEEGTTDSRPKELTKIECLGFLRGSHSPHYDGEADRRPTYHRLIGTGVLKPGYACD
                     NDAGIHFVDNEVRRVVKTREDARVYHVGIQGGEVVERELPAEMIG"
     gene            1946..3412
                     /locus_tag="AC16YU_11710"
     CDS             1946..3412
                     /locus_tag="AC16YU_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR6065937.1"
                     /translation="MSISGPTVRNLAHQALLESAWPAYGSATNPAVALLMQQLSRIWD
                     PLAAGGQGDWLDESRVTFTRNASGRETGLLIEEWHVPSGAWIPSYRQSTTLDSNGLPV
                     ERLDEQWDPDANGGQGGWAPALRVTFTWNDQNRIIVSMTEFWDEDSGDYIIYSRLTTT
                     YDASGERVEQQVFESGLFGPLTPSLRYSFEYDEDGNTVRRLYESYNTLAGEFVNNEDL
                     HYSYDSNGERIEELEFYWDPAANGGAGGWVNYYRTTTAYSPNSSAPTQIVETEQSWDG
                     VGGAWENVERNVLEGSPSLAAPAEIIDTDQVWDPSANGGAGDWLNVDRDVTSFEDLRP
                     VSSVAQTWDPMQTAWVNETRDTVEYDASGNAVSSLGETWNGADWENATLLLMTYEDFG
                     TAIEDENITRGHRLDANYPNPFTESTTISFTLSAPEHVTLEVFDLLGRRVRTVVDADL
                     PPGSHSATVAGESLPAGLYVYRLNGRHFRDSRSMLLVR"
     gene            3536..5623
                     /locus_tag="AC16YU_11715"
     CDS             3536..5623
                     /locus_tag="AC16YU_11715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6065938.1"
                     /translation="MIRLLRPALLGALATLVALPAFAQSRIKSMPGYEQYERVNAERS
                     NAFRSGALSVRWSDDSRSFEYALDGKTWRYDLASKAATEVADAAPESSRRRGGPERGR
                     QYDSADSPDGTLRAFYRDRNLWLSNADGANERQVTTDGNEATRIKNGTASWVYGEELG
                     QLTAMWWSPSGEKLAYYRFDESGVPDFYLQLDQTKLQSTLDVEAYPKAGVDNPVVELF
                     VYDVGSGRTTSIDVRDGRPFTDDVVGHYVYNVAWTPDGTELLVNRTNRWQNILEIAAC
                     SPDTGACRVVVREEWPDSWVSNRPEMEFLEDGRWFIFASERTGWRNYYLYDLDGTLVT
                     PLTEHEFEVAGIELVDETAGVLWYMARSGDNPMKTQLHRVGLDGKGDARVTDPSLNHT
                     VAMSPDGRFFVDVAQAHDYPPVTTLWDVRGPAVAELAISDITGMQALGLEVPELFTFK
                     AADGVTDLYGMLSKPSDFDPSKAYPLLVSVYAGPATNGARETFGLPHPLAEYGFLVAS
                     LDARSAGWRGKRFLDAIYRKLGTVEIDDKAAGVRALRERPYVDGSRVGIFGTSYGGYA
                     SVMAILRHPDVFQAASAASAVTDWRHYDTIYTERYMRTPQSNAAGYDAGSALTYVDSL
                     RGALLIYYGTADNNVHPNNSMQLIHALQEAGKSFDVQVGPDRGHSGINPYRMMEFFID
                     NLVLRPAGPVGSN"
     gene            5719..6882
                     /locus_tag="AC16YU_11720"
     CDS             5719..6882
                     /locus_tag="AC16YU_11720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6065939.1"
                     /translation="MNELPVFYFTRMVLGYRVPVLRRLNERLHNRLVVFSGRPPGSST
                     LKYLEHGEDPGYRHVLLPDHWIGGDRAHFQPFRDAFRKFGTPSVVLAEESPRSVSLPF
                     LLRYARKVGSGRVLWGHFSSLNRRFDPRHNARDRYRLTLARRVEACACYTDGVADLLR
                     PYVPSERLFVARNTIDMAPLAAQYDRLASEGRESVRRRLGLDEEEAVVFFMGRLVPEK
                     GTTLLLETFALLGRDRPATLLIVGDGPDREAMKAWCADRSVDRVRFLGPLSDEDAAPY
                     LFSSDLALMPGYLGLIINHAFAFGLPVVSRADPDGVLYHSPEVEYVRNGENGFLSSGP
                     GPEDLLGAVRAVLRDRERLSANARAFALEKLALDRMVDGLVAAIAYADGHRRS"
     gene            complement(6919..8238)
                     /locus_tag="AC16YU_11725"
     CDS             complement(6919..8238)
                     /locus_tag="AC16YU_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR6065940.1"
                     /translation="MRILVWGLGYVGTVSAACLADLGHEVVGVEVDSTKVEAVNDARS
                     AIKEPGLGELIGKSVGSGRLRAVLEGRELVPWADASLICVGTPSASDGAPSLKHVENV
                     AIEIGEGLRAAGGYHVVVLRSTVFPGTTRSVLVRHLETHSGLKAGRDFGVVMNPEFLR
                     EANALQDFWSPPYTVVGELDDRAGAVAASLYDGIDAPVYRVALEEAEMVKLVNNAYHA
                     LKIGFANEIGRICDSIDVDSHAVMDLVCADTKLNISAAYLRPGFAFGGSCLPKDLRSI
                     TYHARREGVSVPVLDGILPSNRLQFEAARRKVNELGVRSVGVLGLSFKSDTDDLRESP
                     VIDLIRSLWQDGMDVSVHDPNVNPADMMGSNLAYLERQLPQIHEIMRPRISDILENCE
                     AVIVTQKRADFVEALAGLEAKVTVLDLVRVGDRRLTDTIPGYIGLSW"
     gene            complement(8395..9186)
                     /locus_tag="AC16YU_11730"
     CDS             complement(8395..9186)
                     /locus_tag="AC16YU_11730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6065941.1"
                     /translation="MLNPVPALGLVLLAASGCGTAGRDVDPRAVFEHGGVVRGDTLRP
                     EIALVFTGDAYADGGWHIRETLREADVPAAFFLTGNFYRSDDFRDLVSALAEDGHYLG
                     AHSDRHVLYASWEHRDSTLVDADSFARDVLDNYREMARFGIGKEDAPLFLPPFEWYNG
                     ETSEWTRDLGLHLVNYTRGTRSHADYTTPDMGAGYVPGDVIWRSIFEAERSDPNGLNG
                     FILLMHIGTAPERTDKFHLRLGELIGELGALGYTFVRVDDLVLRG"
     gene            complement(9180..>9896)
                     /locus_tag="AC16YU_11735"
     CDS             complement(9180..>9896)
                     /locus_tag="AC16YU_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6065942.1"
                     /translation="NNRARPEAYAMKGFVDDGTVGRVNSAQARWVRRTGIPGFGGWFT
                     NRSLSGGGALIDLLHMIDLALFFMGNPEPAYVLGQTFDEFITDPTFKGPWGIPDVAGG
                     TTDVETAAHGFVTFKTGQVLSLQVSWAEMVEREEVSVTFQGTRAGGKVERLFGRDGLD
                     ETAIDSCELYAQQQGRAVNSSIVVEACEDMGRIRSASNFVLAIEGAEEPLNTPDQALA
                     LMTIIDAIYESAATGRPVAC"
BASE COUNT         1699 a         3350 c         3223 g         1624 t
ORIGIN      
        1 gatcaccgag ccggatcatg ggtcggacat caccacgatg aacaccaccg ccgtacgcga
       61 cggcgacgag tgggtggtga acggcgtcaa gaccttcatc acgaacgccg ggatcgcctc
      121 gttctacgtg gtcgtctgcc agaccgaccc ggaggccggt caccgcggcc agtccatgat
      181 cgtcgttccc gccgacgccg aagggctcgg aacggccgat gtcggcagca agatgggcat
      241 ccgcatgatt ccgacgggcg aactctcgtt cagggacgtg cgtgtgccgg ctgagaacct
      301 ggtcggcgcc gaggggaagg gcttctacca gatcctggag ttcttcgacg agagccgcgt
      361 ggagacggcc gcccagggac tcgggacggc ccagggcgcc ttcgaccgcg cgctcgaata
      421 tgtgaaaagc cgggagcagt tcggcaagaa gatcgcgcag ttccaggcca cgcagcacaa
      481 gctggcggaa atggccacga agatcgaact ggcgcggctc gtgacgtaca aggccgcctg
      541 gaatttcgac cagggccgca tcgacccgaa gctcacgtcc atggcgaaat ggtacgcggg
      601 ccggacggcg gtcgaggtgg cggacgaggc catccagctg ctcggcgggt acggctacat
      661 cgccgagtac gaggtggagc gcttctaccg cgacgccaag atctgcgaga tctacgaggg
      721 aacgaaggag atccagaaga acacgatcgc cggggcggtc atcgggaagc tgaagtagga
      781 ctccgcctat ccgatcatct ccgccggcag ctcccgctcg accacttcgc ctccctggat
      841 tccgacgtgg tagacccggg cgtcctcgcg cgtcttcacc acccgacgga cctcgttgtc
      901 cacgaagtgg atacccgcat cgttgtcgca tgcgtagccg ggcttgagca cccccgtgcc
      961 gatcagccgg tggtacgtgg ggcgccggtc ggcctccccg tcgtagtgcg gcgagtggct
     1021 gccgcgcagg aatccgaggc actcgatctt cgtgagttcc ttcgggcggg agtcggtcgt
     1081 accctcctcg aaccagcaga gtgaccccgc gctggcgccg ccgagcacga tgccccggtc
     1141 ccacgcctcg cggagcaccg cgtcgatgcc gtgcgccttc cagatcacct gctggttgag
     1201 cgtgttgccg cccgaaacga cgatgccgtc catcgacaga agtacctcct cccaggtctg
     1261 gtccatccgg gagctcacga tgaagctctc ctgcacgagg gggtggacgt cgagcggtgc
     1321 gcagtgctcg aagaaccgga tgctcccgta gggcgagtcg gccgatgcgg tgggcaggta
     1381 acagatgcgc ggacgctcct tgccggtcag gccggccatg tagcggatga acgccgtgcc
     1441 gaagccgccg cccgcaatca gaatctggcg cggctcgggc gggccggacg atccggcggc
     1501 gaccgggacg gaggaggagt gagcgccgag cccgatgccg gcgagggcgg tcgtcgtgat
     1561 gaaatccctt cgtttcatgg cagtcctctc gtggggtcgt tggtcccggg cgtctcgtgc
     1621 cgcgagggcc gggatggaca acatactgcc agggcacgac cggaagcgag ccgggcctgg
     1681 tgcgcccggt cctcctgcag aaccgacgaa acggcggagc acctccgttc ccgacccccg
     1741 catgtccgga ctcgtcgatt ccctgcgcgt ctacggaaga cgagatgcca tcttcaccgc
     1801 tacctccact ctcatggctg ctcgaatccg ctggatcgtt gcgatcctcg ctcccctctg
     1861 cctggcctcc ccgtccaatg gccaaccggc cacacgaacc tccggtcacg atttgaccgc
     1921 gattccaggg ggccttcacc cactcatgtc gatctccggt ccgaccgtca ggaatcttgc
     1981 ccaccaggca cttctcgaat ccgcctggcc ggcttacgga tccgccacca accccgccgt
     2041 agccctgctc atgcagcagc tttcccggat ctgggatccc cttgccgctg gcggccaggg
     2101 cgactggctc gacgagagtc gcgtgacctt cacacgcaat gcctccggcc gggaaaccgg
     2161 gctgctcatc gaggagtggc acgtgccgtc gggagcctgg atcccatcct acaggcagtc
     2221 cacgacgctg gactcgaacg gactccccgt cgagcgactc gacgagcagt gggacccgga
     2281 cgccaacggc ggccagggtg gctgggcgcc ggccctccgt gtcaccttta cctggaacga
     2341 ccagaacagg ataatcgtca gcatgacgga attctgggat gaggactctg gcgattacat
     2401 catttactcc cggctgacca ccacgtacga cgcatcgggc gagcgggtcg agcagcaggt
     2461 tttcgagtcg ggcctcttcg gccctctcac cccgagtctc cggtacagtt tcgaatatga
     2521 cgaggacggc aacacggtcc gccggctgta cgaatcgtac aacacgttgg ccggcgaatt
     2581 cgtgaacaat gaagatctgc attacagcta cgactccaac ggcgagcgga ttgaggaact
     2641 ggagttctac tgggatcccg cggccaatgg cggtgcgggc ggctgggtga actattaccg
     2701 gacgaccacg gcctactctc cgaactccag cgctccgacg cagatcgtgg agaccgagca
     2761 gagttgggat ggtgtgggcg gcgcctggga aaacgttgag aggaacgtgc tcgaaggcag
     2821 tccgagcctg gcggcaccgg ccgagatcat cgacaccgat caggtctggg acccgtccgc
     2881 gaacggcggt gccggggact ggctcaacgt cgatcgggac gtcacttcct tcgaagacct
     2941 gcggcccgtg tccagcgtgg cgcagacgtg ggaccccatg cagacggcct gggtgaacga
     3001 gacccgcgat accgtcgagt atgacgcctc ggggaacgcc gtttccagcc tgggtgaaac
     3061 ctggaacggg gcggactggg agaacgcgac cctgctgctg atgacctacg aagacttcgg
     3121 caccgcgatc gaggacgaga acatcacgcg cggtcatcgt ctcgacgcca actacccgaa
     3181 tcccttcacg gaatccacga ccataagctt caccctgtcg gctcccgagc atgtgacgct
     3241 cgaggtgttc gacctgctgg ggcggcgtgt gcggaccgtc gtcgacgcgg atctgccgcc
     3301 cggctcacac agcgccaccg tggcgggtga atcgctgccg gccggattgt acgtctaccg
     3361 tctgaacggt cggcacttcc gcgactcccg gagcatgctg ctcgtcaggt aggcggggat
     3421 cgaccgtcga aggccgaccg gtgcgccaac cgcgtgacca accggtcgta accgctatct
     3481 tgctggaccg gaccgacacc tcgggccgat acgccctgac gaacccgatc cagccatgat
     3541 ccgattacta cgccccgccc tcctgggcgc gctcgcgacg ctcgtagcgc tgccggcctt
     3601 cgcccagtcc cgcatcaagt cgatgccggg ctacgagcag tacgagcgcg tgaacgccga
     3661 acggtcgaac gctttccggt cgggagcgct cagcgtgcgc tggtccgacg acagccgctc
     3721 gttcgagtac gcgctggacg gcaagacgtg gcgctacgac cttgcatcca aggcggccac
     3781 cgaggtcgcg gacgcggctc ccgagtcgtc gcggcgacgc ggcgggccgg agcgcggacg
     3841 gcagtacgat tcggccgact cgcccgacgg cacactcagg gccttctacc gcgaccggaa
     3901 cctgtggctc tcgaacgccg atggcgcgaa cgagcggcag gtgacgacag acgggaacga
     3961 ggcgacccgg atcaagaacg gcacggccag ctgggtttac ggcgaggagc tcggccagct
     4021 gacggccatg tggtggtctc cgtcggggga gaagctcgcg tattaccgat tcgacgagag
     4081 cggcgtcccc gacttctacc tgcaactgga ccagacgaag ctccagagca cgctcgacgt
     4141 cgaggcgtac ccgaaggcgg gcgtggacaa cccggtcgtg gagctcttcg tgtacgacgt
     4201 cggcagcggc cgtacgacgt cgatcgacgt ccgcgacggg cggcccttca cggacgacgt
     4261 cgtgggacac tacgtgtaca acgtcgcgtg gacgccggac gggacggagc tcctcgtgaa
     4321 ccggacgaac cgctggcaga acatccttga gatcgcggcc tgcagcccgg atacgggtgc
     4381 gtgccgagtg gtcgtgcgcg aggagtggcc agacagctgg gtgagcaaca ggcccgaaat
     4441 ggaattcctg gaggacggcc gctggttcat cttcgcgtcc gagcggacgg gctggcgcaa
     4501 ctactacctg tacgacctgg acgggacgct ggtgaccccg ctcaccgagc acgagttcga
     4561 ggtggcgggc atcgagctcg tcgacgagac ggcgggcgtc ctgtggtaca tggcccgctc
     4621 gggcgacaat cccatgaaga cgcagctgca tcgcgtggga ctcgacggca agggtgacgc
     4681 gcgggttacg gacccgtccc tgaaccacac cgtcgcgatg tcgccggacg gcaggttttt
     4741 cgtggacgtg gcccaggccc acgactaccc gcccgtaacc acgctctggg acgtccgcgg
     4801 gccggcggtc gccgagctcg cgatcagcga catcacgggc atgcaggcgc tcggcctcga
     4861 ggtgccagaa ctcttcacgt tcaaggccgc cgacggggtg accgaccttt acgggatgct
     4921 gtcgaagccg tccgacttcg atccgtcgaa ggcgtacccg ctgctcgtca gcgtctacgc
     4981 cggccccgcc acgaacggcg cccgggagac gttcgggctg ccgcatcccc tcgcggaata
     5041 cggtttcctc gtcgcatcgc tcgacgcgcg cagcgcgggc tggcgcggca agcggttcct
     5101 ggatgcgatc taccggaagc tgggcacggt cgagatcgac gacaaggccg cgggcgtgag
     5161 agcgctccgt gagcgaccgt acgtggacgg ctcgagggtc gggatcttcg gcacgtcgta
     5221 cggcgggtac gcatccgtca tggccattct gcgccacccg gacgtgttcc aggcggcctc
     5281 cgcggcatcg gccgtcacgg actggcgcca ctacgacacg atctacaccg agcgctacat
     5341 gcgcacgccg cagtcgaacg ccgccggcta cgatgccgga tccgccctga cgtacgtgga
     5401 cagccttcgc ggcgccctgt tgatctacta cgggacggcc gacaacaacg tgcacccgaa
     5461 caactccatg cagctgatcc atgcgctgca ggaggcgggc aagagcttcg acgtgcaggt
     5521 cggtccggac cgcgggcatt cgggcatcaa tccgtaccgg atgatggagt tcttcatcga
     5581 caacctggtg ctgcggccgg ccgggccggt ggggtcgaac tgaggcagcg gcgagaccgc
     5641 atccgctgca tgccctctcc tgacggcgat cggcggttgg cccacgcggc cgtcatctcg
     5701 cgcccgacaa tcgaaccgat gaacgagctg cccgtcttct atttcacgcg catggttctc
     5761 ggttaccggg tgccggttct gcgtcgactc aacgagcgac tgcacaaccg actggtcgtg
     5821 ttctcgggcc gcccacccgg ctcgtcaacg ctcaagtacc tggagcacgg tgaggacccg
     5881 ggctatcggc acgtgctgtt gcccgatcac tggatcggcg gagaccgggc acacttccag
     5941 ccgttccgcg atgcgtttcg aaagttcgga accccgtctg tcgtccttgc ggaggaatcc
     6001 ccgcgctcgg tgtcgctgcc gttcctgctg cggtacgcac gcaaggtcgg cagcggtcgc
     6061 gtgctatggg ggcacttctc ctcgctcaat cgccggttcg atccccgcca caacgccagg
     6121 gaccggtacc gtctcacact cgcccgacgg gtcgaagcct gtgcctgtta tacggacggc
     6181 gttgccgacc tgctgcggcc ttacgtgccg tctgagcgac tgttcgtggc gcgcaatacc
     6241 atcgacatgg caccgctcgc tgcgcagtat gaccgactgg cctccgaagg tcgggaatcg
     6301 gtgcggcgcc gcctgggact ggacgaagag gaggccgtgg tgttcttcat ggggcgcctg
     6361 gttccggaga aaggcacgac gctcctgctg gagaccttcg ccctgctggg tcgggatcgc
     6421 cccgccacgc ttctcatcgt cggcgacggt ccggaccgag aagccatgaa ggcctggtgc
     6481 gcggaccgtt ccgtcgaccg ggtacgcttc ctcggcccgc tctccgacga ggatgcggca
     6541 ccctatctct tctcctccga tctcgcgctc atgcccgggt acctcgggct catcatcaac
     6601 cacgccttcg ccttcggcct tcccgtcgtc tcgcgcgccg atccggacgg cgtcctgtat
     6661 cacagtcccg aggtcgagta cgtccggaac ggtgaaaacg gattcctgtc gtctggaccc
     6721 ggaccggagg acctgctggg cgccgtccgg gccgtcctcc gggatcggga gaggctgtcc
     6781 gctaacgcac gggcctttgc tctggagaag ctggcactcg accgcatggt cgacgggctg
     6841 gtcgccgcga tcgcatatgc tgacggccat cgtcgctcgt aaccgggagc ccgccagggc
     6901 cacacccggc ggcggagctc accacgacag gccgatgtaa ccgggtatgg tgtccgtcag
     6961 ccgccggtcc ccgacccgca ccaggtccag taccgtgact tttgcctcca gacccgccag
     7021 cgcctcgacg aaatcggctc gcttctgcgt gactatgacg gcctcgcagt tttccaatat
     7081 atccgaaatc cgcggccgca tgatctcgtg gatctgtggc agctgacgct ccaggtaggc
     7141 caggttgctc cccatcatgt cggcagggtt cacgttcggg tcgtggaccg acacatccat
     7201 gccgtcctgc cagagacttc ggatcaggtc gatgaccggg ctttctcgca gatcatccgt
     7261 gtcggacttg aagctgagtc ccagcacgcc cacgctccgc acgccgagtt cgttcacctt
     7321 gcggcgggcc gcctcgaact gaagccgatt gctcggcaga atgccgtcga gcaccggcac
     7381 ggagacccct tcgcgacgcg catgataggt gatggagcgg aggtccttcg gtagacagga
     7441 gccgccaaag gcaaacccgg gacgaaggta ggccgcagag atgttcagct tcgtatcggc
     7501 gcagaccagg tccatgacgg catggctgtc gacatcgatc gaatcgcaga tccgcccgat
     7561 ctcgttcgcg aagccgatct tgagcgcgtg gtatgcattg ttgacgagct tgaccatctc
     7621 cgcctcctcg agggccaccc ggtacacggg agcgtcgatg ccgtcgtaga gcgaggcggc
     7681 aacggctccc gcccgatcgt cgagctcgcc gacgaccgtg tacggtggcg accagaaatc
     7741 ctggagcgcg ttggcctcgc gcaggaactc cggattcata acgacgccga aatcacggcc
     7801 tgccttcagg ccggagtgcg tttcgaggtg ccgaacgagc accgagcgtg tcgtccccgg
     7861 gaagacggtg ctccgcagca ccacgacgtg gtaccccccg gccgcccgca gtccctcgcc
     7921 gatctcgatc gcgacgtttt cgacgtgctt cagggacggt gccccgtccg aggcgctcgg
     7981 cgtgccgaca cagatcagtg aggcatcggc ccacgggaca agctccctgc cctccaggac
     8041 cgcccgcagg cgccccgaac ctacgctctt cccgatcagc tcgcccaggc cgggctcctt
     8101 gatcgcgctg cgcgcgtcgt tgacggcctc aaccttcgtc gagtcaacct cgacgccgac
     8161 gacctcgtgc ccaaggtccg cgaggcaggc tgccgaaacc gttccaacat atccgaggcc
     8221 ccataccagg attcgcattc gccggcccgt ttctggtcag gtgtaacgaa gaccggggaa
     8281 tgtacaacga ccggaagtcg acggggcggc ccggagggat tctccctccc gcgagccatc
     8341 gaattcgggc ctgcttgaca gttcggcaag cccggctcgt cgcgcgccgt gcccctaccc
     8401 ccgcaggacc aggtcgtcga cgcggacgaa ggtgtatccg agggcgccca gctccccgat
     8461 cagctcgccg agccggaggt ggaacttgtc cgtccgctcg ggcgccgttc cgatgtgcat
     8521 cagcaggatg aacccgttca ggccgttcgg atccgaccgc tcggcctcga agatgcttct
     8581 ccagatgaca tcgcccggaa cgtagccggc gcccatgtcg ggcgtcgtgt aatccgcgtg
     8641 gctccgcgtg ccccgtgtgt aattgaccag gtgaaggccg agatcccgcg tccattcgct
     8701 cgtctcgccg ttgtaccact cgaaaggggg cagaaagagc ggggcgtcct ccttcccaat
     8761 cccgaaccgg gccatctccc ggtagttgtc caggacgtcg cgcgcgaacg agtcggcatc
     8821 gaccagcgtg ctgtcccgat gctcccagga ggcgtacagg acgtgccggt cggaatgggc
     8881 ccccaggtag tgaccgtcct ccgcgagagc cgagacgagg tcccggaagt cgtcactgcg
     8941 gtagaagttg cccgtcagga agaaggcggc cggaacgtcc gcctcgcgca gcgtctcccg
     9001 gatatgccac cccccgtccg cgtacgcgtc gccggtgaag acgagggcga tctccggccg
     9061 gagcgtgtcg ccgcggacga ctccgccgtg ctcgaagacg gccctggggt cgacgtcccg
     9121 ccccgcggtg ccgcatcccg aggcggccag caggacgagg cccagggcgg ggaccgggtt
     9181 cagcacgcca ccggcctccc cgtcgcggcc gactcgtaaa tcgcgtcgat gatcgtcatg
     9241 agggcgagcg cctggtcggg ggtgttcagt ggctcctccg cgccctcgat cgcgaggacg
     9301 aagttggagg ccgaccggat gcgccccatg tcctcgcatg cctcgacgac gatggaactg
     9361 ttgaccgcgc ggccctgctg ctgcgcatac agctcgcacg agtcgatcgc ggtctcgtcc
     9421 agaccgtccc gcccgaacag gcgctccacc tttccacccg cgcgggtccc ctggaacgtc
     9481 accgatacct cctcgcgctc gaccatctcc gcccacgaga cctgcagact gagcacctgg
     9541 cccgtcttga aggtcacgaa gccgtgcgcc gccgtttcga cgtcggtcgt tccgcccgcg
     9601 acgtccggga tgccccacgg gcccttgaaa gtcggatccg tgatgaactc gtcgaacgtc
     9661 tggccgagca cgtaggccgg ctcggggttg cccatgaaga agagcgcgag gtcgatcatg
     9721 tggaggaggt cgatcagcgc tccgcctccc gagagcgaac ggttcgtgaa ccatccgccg
     9781 aagcccggga tgccggtgcg ccggacccac cgggcctggg ccgaattgac gcgtcccacg
     9841 gtcccgtcgt cgacgaagcc cttcatcgcg tacgcttccg gacgcgcgcg gttgtt
//
LOCUS       DBNHQZ010000435         5200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_669725, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000435 DBNHQZ010000000
VERSION     DBNHQZ010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_669725"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1061)
                     /gene="gap"
                     /locus_tag="AC16YU_11740"
     CDS             complement(54..1061)
                     /gene="gap"
                     /locus_tag="AC16YU_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6065943.1"
                     /translation="MAIKLGINGFGRIGRLVFRAILERGSSEFDIVGVNDLTDAATLA
                     HLFKYDSVHGRFAGDVSVDGDHLVINGDRFPVFSEKDPASLPWGKLGCDVVVESTGIF
                     RSREKAALHLQAGAKKVVISAPSKGTVDATVVLGVNDSVLTGKEEVISNASCTTNCLA
                     PMVKVLDDTFGLKKGFMTTVHAYTSDQRLHDAPHSDLRRARAAAMSIVPTTTGAAKAV
                     GLVLPHLKGKLDGYALRVPTPDGSLTDLAAELGRETTAEEINAAMKAASEGPLKGIME
                     YSEEPLVSIDIVHNPHSCIFDSLSTMASGSLVKVVGWYDNEWGYASRTVDIVSKLMSR
                     A"
     gene            1351..2049
                     /locus_tag="AC16YU_11745"
     CDS             1351..2049
                     /locus_tag="AC16YU_11745"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6065944.1"
                     /translation="MSTLDPTRKISRRHELREDKVVTFYARAMGLVEKNRGVVLAAGA
                     VVIIVVVAVVGFSMLTAQRDQEAVERMASAVRAWEAGDYRTALDGSDTFMGLTAVIDE
                     YGSTDTGNLAHYYAADAHFQLGEYDQALEHFRAYDKEANHLGAAALAGEAAIMATRGE
                     HEDAGDLYRRASTIFPSDVTSPMYLLRAGQSYEAAGSADDARRAYETIRDEYPASPEA
                     RDIAFFLARVGSRS"
     gene            2065..3453
                     /locus_tag="AC16YU_11750"
     CDS             2065..3453
                     /locus_tag="AC16YU_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6065945.1"
                     /translation="MPVPSILVVDDEAAIRRTLREILEYEDFAIGEAEDGEDALRRVR
                     EDRYDLVLLDVKMPKLDGMEVLQRLSADMPELPVVMISGHGTIETAVEATKLGAFDFI
                     EKPPDLNRLLLTIRNALDRGQLEAENRRMRQTIAERHSTELTPILGDSAPIRQIKETI
                     DRVGPTEARILITGEAGTGKELVARWIHQRSHRSEGPLVEVNCAAIPSELIESELFGH
                     EKGSFTGATKQRIGKFEQAHGGTLFLDEIGDMSLSAQAKVLRALQESSITRVGGDRSL
                     PVDVRVVAATNKNLIAQIDEHRFREDLYHRLSVILVHVPPLRDRRSDIPIITRHIAER
                     IARRNGVEPRPFTDAALEGLKRFEWRGNVRELYNVVERLTILSDGESIGASDVERLVA
                     PGASGAGPMTGLLDRFDQFTDFRDHAERLFIEHQLSLHDWNISRTAEAIGIQRSHLYN
                     KINKYGIERVTD"
     gene            3491..4171
                     /locus_tag="AC16YU_11755"
     CDS             3491..4171
                     /locus_tag="AC16YU_11755"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6065946.1"
                     /translation="MNPLLEATGLQKSYESVSGQPLAVLQGVDLVVEAGTMIAIVGES
                     GTGKSTLLHLLGALDRPDAGSVRFRGKEVFARGDRDLARFRNESIGFVFQFHHLLPEF
                     TAVENVAMPALIAQRPLSEARVRAAALLDLLGLGDRADHRPTQLSGGEQQRVAVARAL
                     MNEPALVLADEPTGNLDVRTGEQLQDELLRLSREMGQTFVVVTHNPAFARRADLVYAL
                     KGGLLQPA"
     gene            complement(4176..>5200)
                     /locus_tag="AC16YU_11760"
     CDS             complement(4176..>5200)
                     /locus_tag="AC16YU_11760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6065947.1"
                     /translation="GGEIVAAEYVGLADRATQRSVDERTIYHWASITKTFTAIALMQL
                     RDRGLVSLDDPVTRYVPEIRGAHNPFGSMDDITIRHLLTHTAGFRAATWPWGGQPWQP
                     YEPTEWSQLVAMMPYTDVAFEPGSKFSYSNLGMVFVGRVIEAVTGEPYEAYVEKNILR
                     PLGMSTAYFDHTPYHLLPYRSNSYFSGPDGVRENGLDFDTGVTVMNGGLNASVPDMAR
                     CLGFLTGVSGVDVLSRASLEEMWREAVPAGTEAGLVQGMGLGFFLLHDGERTFVGHTG
                     SQMGFISFIHVHPETGTGAIGNFNTVGIPPEPGTPPQPDTRGISADIRSLLFSTVFPL
                     FGSDGR"
BASE COUNT          841 a         1744 c         1845 g          770 t
ORIGIN      
        1 ggacctgccg gcaaccgtcc gcgctccccc gaatcgaagg gttgcgtccc ggttcaggcc
       61 ctggacatca gcttggagac gatgtcgacg gtgcggcttg cgtagcccca ttcgttgtcg
      121 taccagccga ccaccttgac gagcgagccg gacgccatgg tcgacagcga gtcgaagatg
      181 cacgaatgcg ggttgtgcac gatgtcgatg gaaacgagcg gctcctcgga gtactccatg
      241 atgcccttga gcgggccctc cgaggcggcc ttcatcgccg cgttgatctc ctcggcggtg
      301 gtctcgcgcc cgagctcggc ggccaggtcg gtcagcgatc cgtcgggcgt gggcacgcgc
      361 agggcgtagc cgtcgagctt gcccttgaga tggggaagca ccaggccgac cgccttggcc
      421 gcgcccgtgg tcgtcggaac gatggacatg gccgcggcgc gcgcccggcg caggtcgctg
      481 tggggcgcgt cgtgcagacg ctggtccgac gtgtaggcgt gcaccgtggt catgaagccc
      541 ttcttcagcc cgaacgtgtc gtcgagcacc tttaccatgg gggccaggca gttcgtggtg
      601 cagctggcgt tggagatcac ttcctccttg ccggtgagca cggagtcgtt cacgccgagg
      661 accacggtcg cgtcgacggt gcccttcgac ggagcggaga tgacgacctt cttcgcgccg
      721 gcctgcaggt gcagggcggc cttctcgcgc gagcggaaga tgcccgtgga ctcgacgacc
      781 acgtcgcagc ccagcttgcc ccagggcagg ctggccgggt ccttctcgct gaagacgggg
      841 aagcggtccc cgttgatgac caggtggtcg ccgtcgaccg agacgtcgcc cgcgaagcgg
      901 ccgtggaccg agtcgtactt gaagaggtgg gcaagcgtgg cggcatcggt caggtcgttc
      961 acgccgacga tgtcgaattc gctggagccg cgctcgagga tggcgcggaa gacgaggcgg
     1021 ccgatgcggc cgaatccgtt gataccgagc tttatggcca tgggtcgttc tcagatcgtg
     1081 agggtcctgc tggtcgatga ccggccgcgg ggcaggtcga aagaaaagtc gcgtacgccc
     1141 ggcgagggtg ctgcgaagat tatggtaaac cgttgaacgg gaagcgcttc cgggcccctg
     1201 aaacggggtg cagggacgat cgcccccggc gttcgccggc cggcagccgg cgggggcggc
     1261 gcgtcggatt gcaggcggcc gagggggagt cgtattttac ttgtctttgc agcacgcacc
     1321 cgactcgcgg cgagcccgcg gcccaaccct atgtccacgc tcgatccgac ccgcaagatc
     1381 tcccgccgcc atgagctgcg ggaggacaag gtcgtcacct tctacgcccg cgccatgggc
     1441 ctcgtcgaga agaaccgggg cgtggtgctc gcagccgggg ccgtggtgat catcgtggtc
     1501 gtggccgtcg tgggcttctc tatgctgacg gcccagcggg accaggaggc ggtagagcgc
     1561 atggcatccg ccgtgcgggc gtgggaggcg ggagattacc ggacggcgct cgacgggagc
     1621 gacacgttca tgggactgac ggccgtcatc gacgagtacg gctcgacgga cacgggaaac
     1681 ctcgcgcatt actacgccgc ggacgcccac ttccagctgg gcgagtacga ccaggccctc
     1741 gagcacttcc gggcctacga caaggaggcc aaccacctgg gcgcggcggc cctcgcgggc
     1801 gaggcggcga tcatggccac ccgcggcgag cacgaggacg cgggcgacct gtaccggcgt
     1861 gcttccacga tcttcccgag cgacgtcacg agcccgatgt acctgctccg cgccggacag
     1921 tcctacgagg ccgccgggag tgccgacgat gcgcgccgcg cgtacgagac gatccgcgac
     1981 gagtacccgg cgtccccgga ggcgcgggac atcgcgttct tcctggcgcg cgtgggctcc
     2041 cggtcctgac ccgcggagcg agccatgccc gtcccctcca tcctggtcgt cgacgacgag
     2101 gccgccatcc ggcggaccct cagggaaatc ctggaatacg aggacttcgc gatcggtgag
     2161 gccgaggacg gggaggacgc gctgcgccgc gtgcgcgagg accgctacga cctggtgctc
     2221 ctcgacgtca agatgccgaa gctcgacggc atggaggtgc tgcagcgcct ctccgcggac
     2281 atgcccgagc tccctgtcgt catgatttcg ggccacggca cgatcgagac ggcggtcgag
     2341 gccaccaagc tgggcgcgtt cgacttcatc gagaagcccc cggacctgaa ccggctgctc
     2401 ctcacgatcc ggaacgcgct cgaccgcggc cagctcgagg ccgagaaccg gcgcatgcgc
     2461 cagacgatcg cggagcgcca ctcgaccgag ctgacgccca tcctggggga cagcgctccg
     2521 atccggcaga tcaaggagac catcgaccgc gtgggaccca ccgaggcccg catcctcatc
     2581 acgggggagg ccggaacggg caaggagctc gtcgcgcgct ggatccacca gcgttcccac
     2641 cggagcgagg gtcccctggt cgaggtcaac tgcgcggcca tcccgtccga gctcatcgag
     2701 agcgagctct tcgggcacga aaaaggctcc ttcaccggcg ccacgaagca gcgcatcggc
     2761 aagttcgagc aggcgcacgg cggaacgctg ttcctggacg agatcggcga catgagcctg
     2821 tcggcccagg ccaaggtgct ccgggcgctg caggagagca gcatcacgcg ggtgggcggg
     2881 gaccgcagcc tgcccgtgga cgtgcgcgtg gtggcggcga ccaacaagaa cctgatcgcg
     2941 cagatcgacg agcaccgctt ccgtgaggac ctgtaccacc ggctgagcgt gatcctcgtt
     3001 cacgtgcctc cgctcaggga ccgccgctcg gacatcccca tcatcacgcg ccacatcgca
     3061 gagcgcatcg cgcgccgcaa cggcgtcgag ccgcgtccct tcacggacgc cgcgctcgag
     3121 gggctcaagc ggttcgagtg gcggggcaac gtgcgcgagc tctacaacgt ggtcgagcgg
     3181 ctgaccatcc tgtcggacgg cgagtcgatc ggcgcctcgg acgtcgagcg cctggtggct
     3241 cccggcgcct cgggggcggg accgatgaca gggctgctcg accggttcga ccagttcacc
     3301 gacttccggg accatgccga gcgcctgttc atcgagcacc agctgtcgct ccacgactgg
     3361 aacatcagcc ggaccgccga ggcgatcggc atccagcggt cgcacctcta caacaagatc
     3421 aacaagtacg gcatagagcg ggtgaccgac tgagtccgcc cgccgcgccg tccgcgggaa
     3481 ccgccaggac gtgaatcccc tactcgaggc cacggggctc cagaagagct acgagtccgt
     3541 ctccgggcag ccgcttgccg tgctccaggg cgtggacctc gtggtggagg ccggcacgat
     3601 gatcgccatc gtaggcgaga gcggcacggg caagagcacg ctgctgcacc tcctgggtgc
     3661 gctggaccgg ccggacgcgg ggtcggtccg attccggggc aaggaggtct tcgcgcgcgg
     3721 cgaccgggac ctcgcgcggt tccggaacga gtcgatcggg ttcgtcttcc agttccacca
     3781 cctgctgccg gagttcacgg ccgtcgagaa cgtggccatg ccggcgctca tcgcgcagcg
     3841 cccgctctca gaggcgcgcg tccgggcggc ggcgctcctg gatctgctcg gtctcggcga
     3901 ccgggcagac caccggccga cgcagctctc cggcggagaa cagcagcggg tggccgtcgc
     3961 acgggccctc atgaacgagc cggccctcgt gctcgccgac gagccgaccg gcaacctgga
     4021 cgtacgcacc ggcgagcagc tccaggacga gctcctgcgc ctgagccggg agatgggcca
     4081 gacattcgtg gtcgtgaccc acaacccggc cttcgcccgg cgcgccgacc tggtctacgc
     4141 cctgaagggc ggcctgctgc agccggcctg acggctcatc gaccgtcgga cccgaagagg
     4201 gggaagaccg tgctgaagag gaggctgcgg atgtccgcgg agatgccccg cgtgtcgggc
     4261 tgcggcgggg tcccgggttc gggggggatg cccaccgtgt tgaagttgcc gatggccccc
     4321 gtgccggttt ccgggtgcac gtggatgaag gagatgaacc ccatctggct cccggtgtgg
     4381 ccgacgaagg tccgctcccc gtcatggagc aggaagaagc cgagtcccat gccctggacg
     4441 agcccggcct ccgtccccgc gggcacggcc tcccgccaca tctcctcgag cgaggcgcgc
     4501 gacaggacgt ccaccccgga cacgccggtg aggaacccga ggcagcgggc catgtccggg
     4561 accgaggcgt tcaggccccc gttcatgacc gtgacgcccg tgtcgaaatc gaggccgttc
     4621 tcccggacgc cgtccggtcc cgagaagtag ctgttggagc ggtagggcag gaggtggtac
     4681 ggggtgtggt cgaagtaggc cgtcgacatc ccgagcgggc gcaggatgtt cttctcgacg
     4741 tacgcctcgt agggctcgcc cgtgaccgcc tcgatcacgc gcccgacgaa gaccatgccg
     4801 agattggaat agctgaactt cgatccgggc tcgaaggcca cgtccgtgta cggcatcatc
     4861 gcgacgagct gcgaccactc cgtgggctcg tacggctgcc agggctggcc tccccagggc
     4921 cacgtggcgg cgcggaagcc ggccgtatgg gtgaggaggt gccggatggt gatatcgtcc
     4981 atcgagccga acgggttgtg cgccccgcgg atctcgggca cgtagcgggt cacggggtcg
     5041 tcgagcgaga ccagcccgcg gtcccggagc tgcatgagcg cgatcgcggt gaacgtcttg
     5101 gtgatggacg cccagtggta gatcgtacgc tcgtccacgg agcgctgcgt cgcccgatcg
     5161 gccagcccga cgtactcggc ggccacgatc tcgccccctg
//
LOCUS       DBNHQZ010000436         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2432780, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000436 DBNHQZ010000000
VERSION     DBNHQZ010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2432780"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16YU_11765"
     CDS             complement(<1..252)
                     /locus_tag="AC16YU_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6065948.1"
                     /translation="MSTTDRPGWMAEIGPLAVVLIWGSNFIIMKAVLAVMHPHAMNAL
                     RLTVSFAVLGLLHWRIRNRAAVPLTRQRIRQIVGVGLVGY"
     gene            364..702
                     /locus_tag="AC16YU_11770"
     CDS             364..702
                     /locus_tag="AC16YU_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6065949.1"
                     /translation="MLGEGITKLYYSIGEVAQLSGEEPHVLRYWESEFPQLHPRKNRA
                     GNRVYTNDDIDLVFRIRRLLREEKYTIDGARQALKREGAAPAPASTDDDLRELREFLG
                     RLLDRIRESG"
     gene            768..1997
                     /locus_tag="AC16YU_11775"
     CDS             768..1997
                     /locus_tag="AC16YU_11775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6065950.1"
                     /translation="MIRVSPRGFALLSLMLLAGLSAGMPAGARAQVTDEALAALVEGI
                     SADSMRASLEVLTGERSFYVGDSLYTEVGGWLYDRTVTIPTRYWATNDLPADWLRRRL
                     ESFGLTAWFHEPVGYPGVTNVLGRIDGVTEPAKQVLVGAHYDAVGDHPGADDNGSGTA
                     AVLEIARLLAGMQPDRTILFALWDAEEIGLVGSERYAQEAKARGDQIIAAINLDMIGW
                     NSGDPGMLVASYDPWGGTWLEARMLQLAQGLELPLDAMPYAMGRSDHVSFATKGYESV
                     MLIEHFGNDFNPYYHSSDDTIDHIDFDYMSAITRLAAAGVAWSAFDALSVGTGVEDLP
                     VAADATAGPWPNPASDRLHFDVRGPVRIELFDVLGRRVLTSPAADVDVSALPVGLYAY
                     RVTGEGRPAAGMVAVSR"
     gene            2069..2142
                     /locus_tag="AC16YU_11780"
     tRNA            2069..2142
                     /locus_tag="AC16YU_11780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:Pro,seq:ggg)
     gene            2365..>3393
                     /locus_tag="AC16YU_11785"
     CDS             2365..>3393
                     /locus_tag="AC16YU_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065951.1"
                     /translation="MRTIPATVLLLSLAAAPAAAQRPTFGATDSVAVQPLSFGFDAEV
                     GLSVFENELGDSERYFSSVFMPMVRKGLWQAGLMLKLRFNGDGMREEDYDETRDYLSI
                     IRFVQYSEKDASGPYGRLGDLEEARLGFGQVVNDYQNTVSLDRPRIGVEFDYDAGAYR
                     MESFVSDVVEVGPYGLRGAWRPFHAEEGSRRQWAVFGVSIAGDVSREARLLNPVSPGT
                     AFLLDPDVLPVDSLGIVPASEGSRPFFVSVDAGMPIRVTGTDEWIGYTTVTRMADHGW
                     GFGVGTEFRRELGTGRLEGQFEQRVSFGEYMPSYFNALYEVDRIRDFGVTLEDGAHLD
                     AANGKLNLL"
BASE COUNT          521 a         1130 c         1189 g          553 t
ORIGIN      
        1 gtacccgacg agacccaccc cgacgatctg ccgtatccgc tgccgcgtga ggggaacggc
       61 ggcccggttc cggatccgcc agtgcaggag ccccagcacg gcgaaggaca cggtgagccg
      121 cagggcgttc atcgcgtgcg gatgcatcac ggccagcacc gccttcatga tgatgaagtt
      181 cgagccccag atcaggacga ccgccagggg tccgatctcg gccatccagc ccggtcggtc
      241 ggtcgtactc atgtgaagct tgaatcgcgg cggccgaaga taccgggcca cagcggaaag
      301 ggggtgcgcg gcgacgggct ttttttcgta gaatgccccg catcaatcca gtcgagcttc
      361 cgcatgctcg gagaaggcat taccaagctc tactactcga tcggcgaggt cgcccagctc
      421 tccggcgagg agccccacgt gctccgctac tgggagtcgg aattcccgca gcttcatccg
      481 cgaaagaacc gcgcgggcaa ccgggtctac acgaacgacg acatcgacct ggtcttccga
      541 atccgccgcc tgctgcgcga ggagaagtac acgatagacg gtgcgcggca ggccctgaag
      601 cgcgagggtg ccgccccggc ccccgcgtcg acggacgacg acctgcgcga gctgcgcgaa
      661 ttcctgggtc gcctgctcga ccgtatcagg gagagcggct gattcctgcc tttcgcggtg
      721 cgcgtccggc ggcgtagcct cgggtcccga tgtccctcgt gcatgccatg atccgagtct
      781 ccccccgcgg gttcgccctc ctttcgctca tgctgctggc aggcctgtcg gcaggcatgc
      841 ccgcgggcgc ccgggcgcag gtcaccgacg aggcgcttgc ggcgctggtc gagggaatca
      901 gcgcggactc catgcgcgcc agcctcgaag tcctgacggg ggagcggtcg ttctacgtcg
      961 gcgacagcct gtacaccgag gtaggcggtt ggctgtacga ccggaccgtg acgattccga
     1021 cgcgctactg ggcgacgaac gacctgcccg ccgactggct gcgccgccgg ctcgaatcct
     1081 tcggcctgac cgcctggttc catgagccgg tcgggtatcc cggcgtgacc aacgtcctgg
     1141 ggcggatcga cggcgtgacg gagccggcga agcaggtgct cgtcggcgcg cactacgacg
     1201 ccgtcggcga tcatcccggg gccgacgaca acggcagcgg gaccgccgcg gtgctcgaga
     1261 tcgcgcggct cctggccggg atgcagcccg accggaccat cctcttcgcg ctctgggacg
     1321 ccgaggagat cggcctggtc ggcagcgagc gctatgccca ggaggccaag gcccgcgggg
     1381 accagatcat cgccgccatc aacctggaca tgatcggctg gaacagcggt gatccgggga
     1441 tgctcgtcgc cagctacgat ccgtggggtg gcacctggct cgaggcccgc atgctgcagc
     1501 tcgcccaggg cctggagctg ccgctggacg ccatgcccta cgcgatgggg cgctccgatc
     1561 acgtttcgtt cgccacgaag ggttacgagt ccgtgatgct catcgagcac ttcggaaacg
     1621 acttcaatcc gtattaccac tcgtccgacg acacgatcga ccacatcgac ttcgactaca
     1681 tgtccgccat cacccggctc gcggcggccg gcgtcgcgtg gagcgcgttc gacgcgctgt
     1741 cggtggggac gggggtggag gatctccccg tggcggcgga cgccaccgcc gggccctggc
     1801 cgaatccggc gtccgaccgg ctgcacttcg acgtgcgggg cccggtccgg atcgagctct
     1861 tcgacgtgct ggggcggcgc gtgctcacga gtcccgctgc ggacgtcgac gtctcggccc
     1921 tgccggtcgg actgtacgcg taccgcgtga cgggcgaagg gcggcccgcc gccggaatgg
     1981 tcgcggtcag ccggtagaaa cgttctcgcc gggaactccg ggcgagtggt tatattagga
     2041 tcgttgcgcg ccacccggcg cgcagcctcg ggacgtggcg cagtccggta gcgcacttgc
     2101 atggggtgca aggggtcgtg ggttcaaatc ccgccgtccc gacatcgggc gccgatttcg
     2161 gcgccggaag ggcgattctg cgagagcggc gtcgcccttt tctcgtgggt accagggagg
     2221 atcgtggcgc aagcgtcaag gtgcgggtac ctggatcggt gaggccatcg ggaatagttg
     2281 ccccggcccg atctgtggga gcgaccgatg gccagtgaca gagcccagcc gctaccttgg
     2341 ggccggcaat cgattcccag gcccatgcgc accatccccg cgaccgtcct gctcctgtcc
     2401 ctcgccgcgg cccccgccgc cgcacagcgc cccaccttcg gcgccaccga ctccgtcgcg
     2461 gtccagccgc tttcgttcgg cttcgacgcc gaggtcggcc tgagcgtgtt cgagaacgag
     2521 ctgggcgatt ccgagcggta tttctcgtcc gtgttcatgc ccatggtccg gaagggcctg
     2581 tggcaggcgg ggctcatgct caagctgcga ttcaacggcg acggcatgcg cgaggaggac
     2641 tacgacgaga cccgcgacta cctgtcgatc atccggttcg ttcagtattc cgagaaggat
     2701 gcctcggggc cttacggccg actcggcgac ctggaggagg cccggctggg attcgggcag
     2761 gttgtgaacg actaccagaa caccgtgagc ctggaccggc cgcgcatcgg ggtggagttc
     2821 gattacgacg cgggggcgta ccggatggag tcgttcgttt ccgacgtggt ggaggtcggc
     2881 ccgtacgggc ttcggggcgc gtggcggccg ttccacgcgg aggagggcag ccggcggcag
     2941 tgggcggtgt tcggcgtgtc gatcgcgggc gacgtgtcgc gggaggcccg ccttctgaac
     3001 cccgtgagtc ccgggacggc ctttctgctg gaccccgacg tcctgcccgt cgattcgctc
     3061 gggatcgtgc ccgccagtga gggatcgcgc cccttcttcg tctcggtcga cgcgggaatg
     3121 ccgatccgcg tcaccggcac ggacgaatgg atcgggtaca cgaccgtcac caggatggcg
     3181 gatcacggct ggggcttcgg ggtgggcacg gaattccggc gcgagctcgg cacggggcgg
     3241 ctcgagggcc agttcgaaca gcgggtcagc ttcggcgaat acatgccgtc gtacttcaac
     3301 gcgctctacg aggtggaccg catccgggac ttcggcgtga ccctggagga cggagcccac
     3361 ctggacgccg ccaacggcaa gctcaacctg ctc
//
LOCUS       DBNHQZ010000437         4508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_401280, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000437 DBNHQZ010000000
VERSION     DBNHQZ010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_401280"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="AC16YU_11790"
     CDS             <1..620
                     /locus_tag="AC16YU_11790"
                     /inference="COORDINATES: protein motif:HMM:NF039288.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6065952.1"
                     /translation="REVKPYLDLADRLARIARQLHGGRAERVVVRCHGDVPRRFGEVL
                     KVSALRGMLSGTVTAPVNLVNAVALADETGLRSDLETWHDPGSYTNLVEVVVEGEGRT
                     LAVAGTIFGEEDPRVVRIDGFSVEVRPEGQLLFYRNVDRPGMLARVGALLAEGSVNIA
                     GLALGRQVRGQTALTAISVDDAIPDSVLERIRGLEGVEEVRQVDV"
     gene            1126..4488
                     /locus_tag="AC16YU_11795"
     CDS             1126..4488
                     /locus_tag="AC16YU_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6065953.1"
                     /translation="MFRKWGMLALALLVTPVMVSAQITGKISGVVTDGETGDPLPGAS
                     VVVLGTTLGTITDVDGNYFIIGVPIGEFDVQASFVGYQTSTVAGVGISGGYTRELNFT
                     LSPGVELDEIVVEYERPLIQKDAIGVPQVVSAEDIQNLPVRGAANVAALQGGVVSTET
                     SSNLYIRGGREQEVAYVVDGIGVIGAVAVPTQAIQEQEMLIGSIPARYGNAMSGIISI
                     TTKEGGASNFFGSIEAITSEVLDSYGYNTLSATVGGPLAGRKASFFLSGQYDSRGAAA
                     RAGGFPQLSEGDYALLQENPQVIEFHDTHGEGLVAYVPVPSAALAALAGEDGTIPDGD
                     AFDAIIAAAEAAGYSPDDWGLASATPIFAPLTYTADRWTFQDEYPSSTSDALALNGNV
                     VLSPSDAVRLRVVGAFETSKGDNTSNIHSLYNTATTPYGQYERDTYRIGATFTHYISS
                     STFYQLGGNFSDYEGVDYRTGFSNKVEDVLFYGDISHPANAVAARYYTYDEANGHFQQ
                     EYSDGSLPNLRDIHSTWAPPGAGTAGFSKFHNTSLSLRASATTQVGLHQIDFGVNWSQ
                     SKNRYFSGGSYALSRYYNDGDVEAGADEAVDSYDELSFGVMDTRVFYYGYDYLGLNEV
                     DDQDVDAFAAGENYNIEPYQPIYYAGYIQDKIEFRDIVLQIGARLDVFDNNALVLRDP
                     YSLFEITRAGDLTSNVPSNIKSNYSVFFDESTGEIRGYRDLEGQFYDPEGNSVVSRDV
                     TFGAAVQIKTDESGAEMKRLTSNVFKEYEPQVEFQPRIGVSFPVTDQALFYASYDVVT
                     QRPSENVYDTIHQYKQATEFSKRNNNPNLKPERTTQYELGFRQRLGARAAIQLSGFYR
                     QIENKIALRIFQNVTPGNYQSYQNVDFGTVKGVELEFDLRRTQGLALTANYTLSFAKG
                     TGADAASTGQIVWRQETLPFYPNFLSFLDFDQRHTANVTLDYRLGQGEGPSIGGSRPL
                     QNFGVNLVATVGSGLPYTRRDDTSPLYTAFNGFLEGELNSSRLPSTTMLNLRVDRRFP
                     LTGRTNLVVYLWVQNLLDTKNVLAVYPQTGLAGDDGYLSGPEGRDQVAAIANSQGALL
                     ADSFADHYGIAADNPFRYGIPRQTRIGLRLDF"
BASE COUNT          812 a         1480 c         1470 g          746 t
ORIGIN      
        1 agcgggaggt caagccctac ctggacctgg cagatcggct ggcccgcatc gcgcggcagc
       61 tccacggcgg acgcgcggag cgggtcgtgg tccgctgcca cggggacgtg ccgcggcgct
      121 tcggcgaggt gctcaaggtc tcggcgttgc gcgggatgct ctcgggaacc gtcaccgcgc
      181 cggtcaacct ggtcaacgcc gtggcgctgg cggacgagac cggcctgagg tccgacctcg
      241 agacctggca cgatccggga agctacacca acctcgtcga ggtcgtggtc gagggcgagg
      301 gtcgcacgct cgccgtggcg ggcaccatct tcggagaaga ggacccgcgg gtggtccgga
      361 tcgacggctt ctcggtcgag gtccggcccg aaggccagct gctcttctac cggaacgtgg
      421 accggccggg aatgctcgcc cgggtcggag ccctgctggc cgagggaagc gtcaacatcg
      481 ccgggctcgc actcgggcgg caggtgcggg gccagaccgc gctgacggcc atcagcgtgg
      541 acgacgcgat tcctgattcc gtcctcgagc gcatccgcgg gctcgagggg gtggaggagg
      601 tccggcaggt cgacgtgtga aggcggaacg gcaagtggtt tccggtgtcg ggggcatggg
      661 gtccgctccg tagggccatg aggggggatc cccgatcgtt tcgggagagg ccgatccgtg
      721 acgtgcaaaa tcgagccgaa aacagggcat tttcccgcga ctgcaaatcc ttcgccctgc
      781 actcacacag ggttcacgtt gccttgacaa tagaaaatga cccgtctata tttgccgggc
      841 attacggacc cacaaccagc gcggcaccgc atcggaccat cgctacggca cttcgaaaca
      901 ccttgcgtgc aattgtgaac ggggacaacg ccgcacacgc ggcgcgtgat ggggaagtcg
      961 acacgccgtc ctatgccggg cgcacccgcg ctatcagact gtacggcatc ttcaatccca
     1021 ggttgcgtga acccgtcctt cgtgccgatg cacgatccgc ttcgtacggc gtcgggtcgt
     1081 accacaccct gtaagccaca caccatacac aggaggaggt agcttatgtt tcgtaaatgg
     1141 ggaatgcttg cgctcgccct gctcgtcaca ccggtgatgg tctctgccca gatcacgggc
     1201 aagatctccg gcgtcgtgac ggacggcgag accggcgacc cgctccccgg ggcaagcgtc
     1261 gtcgttctcg gcacgaccct cggcacgatc accgacgtcg acggcaatta cttcatcatc
     1321 ggtgttccga tcggtgagtt cgacgttcag gcgtcgttcg tcggctatca gacgtcgacc
     1381 gtggcgggag tcgggatctc gggcggttac acccgggagc tgaacttcac gctgtcgccc
     1441 ggcgtcgagc tcgacgagat cgtcgtcgag tacgagcggc cgctcatcca gaaggacgcc
     1501 atcggtgtcc cgcaggtggt gtctgcggag gacatccaaa acctcccggt tcggggcgct
     1561 gcgaacgtgg cggcgcttca gggcggcgtc gtgagcacgg agacgtcgag caacctctat
     1621 atccgcggcg gccgcgagca ggaggtcgcc tacgtcgttg acggcatcgg ggtgatcggt
     1681 gccgtggcgg ttcccacgca ggcgatccag gagcaggaga tgctcatcgg ctccatcccc
     1741 gcccgctacg gcaacgcgat gtccggcatc atctccatca cgaccaagga gggtggcgcg
     1801 tccaacttct tcggatcgat cgaggccatc acgtcggagg tgctggattc gtacgggtac
     1861 aacaccctgt ccgcgacggt cggtggaccg ctggcgggtc ggaaggccag cttcttcctt
     1921 tccggtcagt acgacagccg cggcgccgcg gcccgggccg gcggcttccc gcagctctcc
     1981 gagggcgatt acgcgctcct ccaggagaac ccgcaggtca tcgagttcca cgacacgcac
     2041 ggcgagggcc tggtggcata cgttccggtg ccttccgccg ccctggccgc cctggccggt
     2101 gaggatggga cgatccccga cggcgacgcc tttgacgcca tcatagcggc ggcggaagcc
     2161 gccggataca gcccggacga ctggggcctg gccagcgcga cgccgatctt cgcgccgctg
     2221 acgtacacgg ccgaccgctg gaccttccag gacgagtacc cttcctcgac gtccgacgcg
     2281 ctggcgctga acggcaacgt ggtcctcagc ccgtcggacg ccgtccggct ccgcgtggtc
     2341 ggcgccttcg agacctcgaa gggcgacaat acgtcaaaca tccactcgct ctacaacacg
     2401 gcgacgacgc cgtacgggca gtacgagcgg gatacgtacc gcatcggggc cacgtttacg
     2461 cactacatct cgagctccac gttctaccag ctcgggggca acttctccga ctacgagggc
     2521 gtcgattacc gcacgggttt ctcgaacaag gtcgaggacg tcctcttcta cggtgacatc
     2581 tcgcacccgg ccaatgcggt cgccgcgcgg tattacacgt atgacgaggc caatggccac
     2641 ttccagcagg agtacagcga cggcagcctt ccgaacctca gggacatcca ctccacgtgg
     2701 gcgcctcccg gagcgggcac ggcgggcttc tccaagttcc acaacaccag cctcagcctt
     2761 cgtgccagtg ccacgaccca ggtcgggctg caccagatcg atttcggcgt gaactggtcc
     2821 cagtcgaaga accggtactt ctccggcggc tcctacgccc tgtcgcggta ctacaacgac
     2881 ggggacgtgg aggccggcgc cgacgaggcg gtcgacagct acgacgagct ctcgttcggc
     2941 gtgatggaca cccgcgtctt ctactacggg tacgactacc tcggcctgaa cgaggtggac
     3001 gaccaggacg tggacgcctt cgccgcgggc gagaactaca acatcgagcc gtaccagccg
     3061 atctactacg ccggctatat ccaggacaag atcgagttcc gggatatcgt gctgcagatc
     3121 ggcgcccggc tcgacgtctt cgacaacaat gccctggtcc ttcgcgaccc gtactcgctg
     3181 ttcgagatca cgagggcggg cgacctgacg tcgaacgtcc cgtcgaacat caagagcaat
     3241 tacagcgtct tcttcgacga gtcgaccgga gagatccgtg gctatcgcga cctggagggt
     3301 cagttctacg atccggaagg caactcggtg gtctcccgcg acgtgacgtt cggtgcggcg
     3361 gtccagatca agacggatga gagtggcgcc gagatgaagc ggctgaccag caacgtgttc
     3421 aaggaatacg agccgcaggt cgagttccag ccgcgcatcg gcgtgagctt tccggtcacg
     3481 gaccaggccc tcttctacgc cagctacgat gtggtcacgc agaggccgtc ggagaacgtg
     3541 tacgacacga tccaccagta caagcaggcc acggagttct ccaagcggaa caacaacccg
     3601 aacctgaagc cggagcgtac gacgcagtac gagctcggat tccgtcagcg cctcggcgcg
     3661 cgggcggcga tccagctgtc cggtttctat cggcagatcg agaacaagat cgcgctccgc
     3721 atcttccaga acgtgacgcc cggcaactac cagtcctatc agaacgtgga cttcggcacg
     3781 gtgaagggcg tcgaactgga gttcgacctg cggcggacgc agggcctggc actgaccgcg
     3841 aactacaccc tgtccttcgc gaaaggcacc ggtgcggacg cggcctcgac cggacagatc
     3901 gtgtggcgcc aggagacgct tccgttctac ccgaacttcc tcagcttcct ggacttcgac
     3961 cagcggcaca cggcgaacgt gaccctggac taccggctgg gccagggcga gggaccgtcg
     4021 atcggcggct cgcggccgct gcagaacttc ggggtgaacc tggtggcgac ggtcggctcg
     4081 ggcctgccgt acacgcggcg cgacgacacg agcccgctgt acaccgcgtt caacggattc
     4141 ctcgagggcg agctgaacag ctcgcgcctg ccgtcgacga ccatgctgaa cctgcgcgtg
     4201 gaccggcgct tcccgctcac cggccgcacg aatctcgtgg tctacctgtg ggtgcagaac
     4261 ctgctcgaca cgaagaacgt gctggcggtc tatccgcaga caggcctcgc gggagacgac
     4321 ggatacctct ctggtccgga agggcgtgac caggtcgcag ccatcgcgaa ctcgcagggt
     4381 gcccttctcg ccgattcctt cgcggaccac tacgggatcg cggcggacaa cccgttccgg
     4441 tacggcattc cgaggcagac ccggatcggt ctccgtctcg acttctgatt cggaccgggg
     4501 cgcggggg
//
LOCUS       DBNHQZ010000438         3369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2704437, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000438 DBNHQZ010000000
VERSION     DBNHQZ010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2704437"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="AC16YU_11800"
     CDS             complement(<1..725)
                     /locus_tag="AC16YU_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065954.1"
                     /translation="MKRSGFVRFQVLALLVLAGLLVWRATRGPELPEAAVSLTGLEVG
                     ELYSSAFSLGTAGRVRLDAAGSVESPAHPVPAAYAWILGADRSVVWRADADSLPPDGT
                     GFAVVDTILLEAGIHQAYFTTLGPTPESRSDRPFLGLKRHWTNDARSWRFLLSPVDSI
                     RISGVDEAPESPPDDAPLWTSGPVRNRSTRDTWLAVDSPTSIRVRAVAGVCDSGCDEA
                     MIEDAVTGQTVWSLSGAESQPAGG"
     gene            984..3176
                     /locus_tag="AC16YU_11805"
     CDS             984..3176
                     /locus_tag="AC16YU_11805"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6065955.1"
                     /translation="MTSAVARARSLMIGLVGAASLVVAIGFHVLAWNAGRPVGGLLLG
                     VFHALTFVGLGSLWIFLRGLVERHRPGPARSFWSLSLVAAGAAALTFLALLVAGPPAE
                     AQLPASVLMGFDLDTGAPIVLATVLKMSVLAAIQGAFAVFLLVRLRGLVLVKRTRSSV
                     RNWKIMVALMVVAAVAVGGSPPEAEMNEVQTILVICAIVFMLVNAFRLSWIVSLSFRQ
                     KMATAGLSLVLLGILSVGQGVMSSGAPQSGIVAGYQGYVQHFSMPLALFSTQASMFGI
                     LYCLTTMLSLLFHLPTSGEFQQRAGERAAMHALTHSVGGVFDSDRLHAAIAGSPVEAG
                     SAHSAWLSIADPDSGSLRPRVVAAHGVDIDRIELLIDHEALYADLLARRVPLVLDHAL
                     ADHRIRARPGDGVDSLVVAPLLARDHMVGALFACKQVEMGFEKDEVETIAILAGQAAL
                     AIENARLFEQQVEKERLARELAIAREVQRRLLPQEIPVLEGVSMAASSVSAQEVGGDY
                     YDFVRLGANRLGIIIADVSGKGTSAAFYMAEMQGIFQAVSRLAPSPAEFLYHANVAIS
                     ESLERNTFVSAVYGVLDVEAETLVLARAGHCPVAVVNVNGEGSFLRSGGLGLGLDRGE
                     TFRDSVVEIDLRLAPGDVYALYTDGVVETRNRDGEEFGYDRLLRVLAANRHEEAGTVH
                     AAMIDELTRFADGLEYGDDMTLVILKWHGLPTASVASGEIVAERAGYA"
BASE COUNT          495 a         1175 c         1151 g          548 t
ORIGIN      
        1 ccgccagccg gctgcgattc ggccccggag aggctccaga ccgtctgccc ggtcaccgcg
       61 tcctcgatca tagcctcgtc gcagccggag tcgcagaccc cggcgacggc cctgacacgg
      121 atcgaggtcg gagagtccac cgcgagccag gtgtcgcggg tcgagcggtt ccggacggga
      181 cccgacgtcc agagcggagc gtcgtcgggt gggctttcgg gagcttcatc cacgccggat
      241 atccggatgg agtccacggg agagagcagg aagcgccacg atcgcgcgtc gttcgtccag
      301 tgccgcttca gtccgaggaa ggggcgatcc gaccgcgact ccggggtcgg tccgagggtc
      361 gtgaaatagg cctggtggat gcccgcctcg agcagaatgg tatcgacgac ggcgaacccc
      421 gtgccatcgg gaggcagcga atcggcgtcg gcgcgccata cgacggaccg gtccgcgccc
      481 aggatccagg cgtaggccgc cggcacggga tgcgccgggc tctcgaccga gcccgccgcg
      541 tcgaggcgca cgcgacccgc cgtcccgaga ctgaacgccg aggagtacag ctcgccgacc
      601 tccaggccgg tgagcgagac ggccgcctcc ggcagttccg gcccccgggt cgcccgccag
      661 acgaggagcc cggccagcac gagaagcgcg agcacctgga agcggacgaa tccggacctc
      721 ttcattgcgt cggacagtga aacatcggcc ggatgatacg tcgaccatgg ccggaagtta
      781 ccccgggacg gccccggcag gagggcttcc gcggctcctc gagcgcggcc gcggcggcgt
      841 ttctccgttc gcggacgctt ccctaccttg aagtttgtca tgtaacaaag gtctccggcg
      901 ccgagtagaa cgcaccggca gtccgatttg ccgggccgtg cgtccggcac caacgcatga
      961 tcccggtccc aaggctgaca cgaatgacga gtgccgtcgc gcgcgcacga tccctgatga
     1021 tcgggctcgt cggcgctgcc tcgctcgtgg tcgcgatcgg gttccacgtc ctggcctgga
     1081 atgccggccg accggtcggc ggcctcctgc tgggggtgtt ccacgcgctg accttcgtcg
     1141 ggctcggctc cctctggatc ttcctgcggg gcctggtgga acggcaccgg cccggtcccg
     1201 cccgttcgtt ctggtccctg tcgctcgtcg cggccggagc ggcggcgctc acgtttctcg
     1261 ccctcctggt cgccgggcca cccgcggagg cccaactgcc ggcctccgtg ctcatgggct
     1321 tcgacctgga caccggggcg cccatcgtgc tcgcgaccgt cctgaagatg agcgtgctcg
     1381 cggcgatcca gggcgcgttc gccgtgttcc ttctcgttcg gctgcgcggc ctggtgctgg
     1441 tcaaacgcac ccgatcgagc gtcaggaact ggaagatcat ggtggccctg atggtggtcg
     1501 cggccgtggc cgtcggcggc tcgccccccg aggccgagat gaacgaggtc cagaccatcc
     1561 tcgtgatctg cgccatcgtg ttcatgctcg tcaacgcgtt ccgcctgtcg tggatcgtgt
     1621 cgctgagctt ccggcagaag atggcgaccg cggggctgag cctggtcctg ctcggcatcc
     1681 tctcggtcgg gcagggcgtg atgagcagcg gcgcgcccca gtccggcatc gtggcgggct
     1741 accagggcta cgtgcagcac ttcagcatgc cgctggcgct cttctccacg caggcctcca
     1801 tgttcggcat cctgtactgc ctgacgacga tgctttcgct gctgttccat ctcccgacat
     1861 ccggggaatt ccagcagcgg gcgggcgagc gcgcggccat gcacgcgctg acccactcgg
     1921 tcggcggcgt cttcgattcg gaccggcttc acgcggccat cgccggctcc ccggtcgagg
     1981 cgggatcggc ccattcggcc tggctttcca tcgcggatcc cgacagcggc tcgcttcggc
     2041 cccgcgtggt cgcggcgcac ggcgtggaca ttgaccggat cgagctcctc atcgaccacg
     2101 aggcgctcta cgcggacctc ctggcccggc gtgtcccgct cgttctggac cacgcgctcg
     2161 ccgaccaccg gatacgggcg cggccgggcg acggcgtgga cagcctggtg gtcgcccccc
     2221 ttctcgcgcg cgaccacatg gtgggcgcgc tcttcgcctg caagcaggtg gagatgggct
     2281 tcgagaagga cgaggtcgag accatcgcca tcctggcggg ccaggccgct ctcgcgatcg
     2341 agaacgcacg cctcttcgag cagcaggtcg agaaggagcg gctcgcccgt gagctcgcga
     2401 tcgcgcgcga ggtccagcgc cggctccttc cgcaggagat ccccgtgctc gagggcgttt
     2461 ccatggccgc gtcgtcggtc tcggcgcagg aggtcggggg cgattactac gatttcgtga
     2521 gactgggcgc gaaccgcctg ggcatcatca tcgccgacgt gtcgggcaag ggcacgtccg
     2581 cggccttcta catggccgag atgcagggga tcttccaggc cgtgagccgc ctcgccccct
     2641 ccccggccga gttcctttac cacgccaacg tcgccatctc cgaatcgctc gagcgcaaca
     2701 cgttcgtttc cgcggtgtac ggagtgctgg acgtcgaggc cgagaccctg gtgctcgccc
     2761 gggcgggtca ctgccccgtt gcggtggtca acgtcaacgg cgagggctcc ttcctgcggt
     2821 caggcggcct cgggctgggg ctggaccggg gcgagacctt ccgcgattcc gtcgtggaga
     2881 tcgacctgcg cctggctccc ggcgacgtgt acgcgctgta taccgacggc gtcgtcgaga
     2941 cgcggaaccg cgacggcgag gagttcggtt acgatcggct gctgcgcgtc ctcgccgcga
     3001 atcgccacga ggaggccggc accgtccatg ccgccatgat cgacgaactg acgaggttcg
     3061 ccgacggcct ggaatacggc gacgacatga ccctcgtcat cctgaaatgg cacggactgc
     3121 ccactgcatc ggtcgcctcc ggggagatcg tggcagaacg tgccggttac gcgtagctta
     3181 cgcgccgccc agccggaccg gcggctccgg cccaatcgtc atccgaagaa ccccaacgaa
     3241 atcatgagca acttctccgt cggcttcagg tcctccggaa gcgtgcagat cctggacctg
     3301 cgtggagagc tcgacgcgca caccgccgaa gaactcgagg cggccatgga gaaatgccac
     3361 ggcgaccag
//
LOCUS       DBNHQZ010000439        14967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2568518, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000439 DBNHQZ010000000
VERSION     DBNHQZ010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2568518"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16YU_11810"
     CDS             complement(<1..268)
                     /locus_tag="AC16YU_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR6065956.1"
                     /translation="MCLVLVALGIRSDAPLVIAANRDEFHARPTAPAAYWPEPAGMLA
                     GRDLLHGGTWLAVARSGRFAAVTNIREPSHHRRESRSRGELPVQF"
     gene            385..900
                     /locus_tag="AC16YU_11815"
     CDS             385..900
                     /locus_tag="AC16YU_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065957.1"
                     /translation="MRFSSIRLVSAALLAMVLLWAPDSRAQTTRMGVHAGLNLDGSEV
                     FIGLNGQFGITISDRQALVGLTGELYPFIDNMSVTVVELDALFPFRVSAVELYAGGGL
                     ALRMRRLDLPSDSLADDSDTDVGLNIKVGAVFGNEEAGIRPFVELDQTIGAGTDLAAR
                     AGVFFMLGGGR"
     gene            921..2024
                     /gene="aroB"
                     /locus_tag="AC16YU_11820"
     CDS             921..2024
                     /gene="aroB"
                     /locus_tag="AC16YU_11820"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6065958.1"
                     /translation="MSDSDHARIDVELPSGVRYPIVFGPLDGLPSGMARHGLRQGRCV
                     ILSDENVAALYGDSVSGAFSRDGWDVRLVVLPAGESTKSLEHFGMVLDAVLPWGIDRS
                     TPLVALGGGVVGDIGGFAAASLLRGLPLVHAPTSLLAQVDSAIGGKTGINHPAGKNLV
                     GAFHQPRLVWSDAAVLTTLPRREWTGGVAEVVKHALIEGDPLLERLEARLDRFLAADP
                     AVVPALIREAAAVKARIVEIDERESGIRAFLNFGHTFAHALERALGYGTLTHGEAVAV
                     GMRAALHLSRSRRPTLAFERAVAMVDSIPVPAIPGSLGLDELAGAMRLDKKADGRRLR
                     FVLLDELGSPCLVDDVGPEEVEAAWRFALRRGA"
     gene            2086..6765
                     /locus_tag="AC16YU_11825"
     CDS             2086..6765
                     /locus_tag="AC16YU_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6065959.1"
                     /translation="MFQFLRRLPGRTLRGLLALALVAVVALVALTRTQVGRDALARRI
                     ESEFAETYDGALTIGRLTGNLMREFVASDITVVDGAGGESLHIDSLLVRTSWEDLVRR
                     RIVVPRIVLVRPSVRIQVGDSGRYRVPGFARRVPAADSVDGFLRFRSARIQVADGRLV
                     TEPSDSARVRPERPFFADPAGLRIEGVALDALLDWSATQRQFDLFQLSARGVTPQIEL
                     RSAETQVILQDDRISINRLRIALDRSRLAAEGMILLRENEVSEVDLSVEAPEVSFDEL
                     RSAIPGLPLAGSGAFGIRLSGSPGGIAVSRLEFATRESFLVASGTAVGLPDSLDFEIS
                     ADRIHARSGDLRALLPDAAFLRSASFDSLGASGYARGVAWVTGASVDSLRSDGSLVLN
                     GDAGSVASTFSVDLRDRSRPLVSGDAELDGLDLAAFLGDRTTRLTGRLEVATEGLLPR
                     TMTGDVRVELADSRIGNRHVEHLVAHASAEGTALRFDVAMDTQAAGWLRADGLWTRDS
                     LGTLSADAAWSRFDVAPLLGTDSLHTAATGRLGALFTGHDEASFAGVLRLEIDSTVVR
                     IGGRESLVLPQRHRLDLARADAPVPRIRLSGDALDAEIHGDVRLEALSAVASFWTDAI
                     AGAVRRELDKPYPGTAPDPASQLVENARDIRTSVLRGGAEQALADLGLGRPLELRIDA
                     TVRRPELLTGWIPGLPNVRGRIQVSADARADADSLGLSMTLSGDTVRVDSTGLLNPRA
                     RFRATTAFDQAVERSLRLDGGLVADSIASGPVLLPSIDVSLRIADGRARIAVDAGRHD
                     DIGPLHVRAEVESRPDRNRLEIREASLDARNYAWTLSRPATIDLYAGGITVEDLFVES
                     DYSDLYGNQSVAVHGAYSGLATDTLFVEADGVRLEELTRFASLKPALGGRLDGQAAFT
                     RSNGRPLLVGDATTTGLVLDGRLIGHVVARSRLVPGSPEVSIRLDLTPADTAGFRWPQ
                     PPREVRRNALTVTGTVRLPVADEGDPGEWNLDVRASRADLFFLKYIFNETLEDVGGYL
                     AGEGRVTGSISRPVFDVDFTASEGTFYVPVTGVRYTLEGDVGLTRDAIVFPGVTVRDA
                     GGGSALVTGSLYFNEYEYFSFGLNAALDELQIINTGYTDELPFYGSIRGSGSATLTGP
                     LAAAYLRVPDGVARDDSELFIPIVESATATDDSFIVFTDSTGLVPDLRELARRPFLLA
                     RRATMERRFLDGLDIDINLFAPRGSVVHLVIDPLLGDVINAESTGRVQIRREQGEFEI
                     FGTLEVEGGDYLFTAGEVFVRRFIIEEGGSISWESDPIDARLDIPAVYRTRASWAGLD
                     AANTGSSALIPLVVELRITGRVMSPAVELSLAIDRTNQNVLGDYQALEARLNNPERAA
                     EYATSVLLTNSFQLTTETVDSRTGEQLAFNSVSQLVSAQLNRFLNAALPNVDVSFGLL
                     GERASDLDITYGVALRLLDERLIIRGEGVYQGSRTDATNVAAETMQGEVVVEVRLSPT
                     VSVEVFFRREGDVFQSAELTNTTGAGLSYQTEFSTWRSLFDRLFGWLTPDPEPTESPT
                     SGSGELR"
     gene            complement(6758..7210)
                     /locus_tag="AC16YU_11830"
     CDS             complement(6758..7210)
                     /locus_tag="AC16YU_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6065960.1"
                     /translation="MSGISRIEFLSGLSEWFGHEGPDLPGGVERPASGARPVLRGADF
                     VRSARRAVEDGLDRPSFGVADLARALRLSPRQLQRRIQDSAGCSPCQFIRTIRLERAR
                     SLIEDGRQSVGDAAQAVRISSLSYFARRFREEFGLNPSEFGLNPDSTT"
     gene            complement(7401..8396)
                     /locus_tag="AC16YU_11835"
     CDS             complement(7401..8396)
                     /locus_tag="AC16YU_11835"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase"
                     /protein_id="HIR6065961.1"
                     /translation="MAEPHRLQPELETATGAVRAAARLCLNVLREMELMPVRKRDSSP
                     VTVADYGSQALICREIGRAFPGATVVAEESSAELSGAGHPGLSDSVLAHVRAFHPSAA
                     AEDLARWIDMGSGGEVTSRYWTVDPVDGTKGFLRGNQFAVVLALVEDGRPVLACLACP
                     RLELGDTCGVLAWALRGEGAYVEPLFGGGTAERLRVRATTDASAARYCESVEHTHSSH
                     DDAARLAEALGIAEPPIRMDSQAKYAVVAHGDAEIYLRLPSGRRFVENVWDHAAGSLL
                     VEEAGGRVTDISGAPLDFGHGRRLTMNRGVIATNGLLHDQVLGALRSSSGESPGG"
     gene            complement(8389..8877)
                     /locus_tag="AC16YU_11840"
     CDS             complement(8389..8877)
                     /locus_tag="AC16YU_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HIR6065962.1"
                     /translation="MREDFAEMAVSLPKLNLPAPPDVRLRRRQRGTEIFDRVRRKWVA
                     LTPEEWVRQHAVHLLLERVGVAPGLVAVERELVWEGTRRRVDIVAYGRDGRPWMVVEC
                     KAPAVSVGQDAMDQAGRYARSMGAAFVVVTNGLQLVGWQTLPGGGSLAMEELPQAPTR
                     DG"
     gene            complement(8909..9844)
                     /locus_tag="AC16YU_11845"
     CDS             complement(8909..9844)
                     /locus_tag="AC16YU_11845"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR6065963.1"
                     /translation="MHHRHLLGLRTWSADEIGLVLDTARQFREVLDRPIRRVPTLRGV
                     TVVNLFFEPSTRTRISFELAEKRLSADTVNFSASISSVTKGETLKDTAQNIEAMRIDM
                     VVIRHASPGAPQFLTQCVDAVVINAGDGAHEHPTQGLLDLLTISDRFESLDGLNVSII
                     GDITHSRVVRSDLYGLTRLGANVTLCGPPTLMPPGIEHLDAGGRVRIVRGLDEALEGA
                     DVAIALRIQLERQGKTLLPSLREYHETFGIRMEHLERHPDLLVMHPGPVNRGVELASE
                     VVDHERAIILNQVTNGVAVRMAVLYLLSGHGGEAA"
     gene            complement(9905..10477)
                     /gene="pyrR"
                     /locus_tag="AC16YU_11850"
     CDS             complement(9905..10477)
                     /gene="pyrR"
                     /locus_tag="AC16YU_11850"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR6065964.1"
                     /translation="MEPQDVVKAQLMDERDLDRTLDRMACRILEYAAPGTDPSETMGI
                     VGMQSRGVHLARRLQRKMHDLDGIDCPMGVLDATMYRDDFRLRLKQPAVRETHVPFDV
                     SERHLFLIDDVLYTGRTARAALDALMDLGRPASVHFLVVVDRGLRELPIRADVVGRHV
                     PTLPGEEVRVRLREEDDREGVWLVTTPRAR"
     gene            10612..11544
                     /locus_tag="AC16YU_11855"
     CDS             10612..11544
                     /locus_tag="AC16YU_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6065965.1"
                     /translation="MNTQDPVFRYLDPTVVSQLRNMELRARLIVEGFITGLHKSPYHG
                     FSVEFAEHRPYNPGDELRHVDWKVYAKTDRHYVKQYEEETNLRHYVVLDTSPSMRYRG
                     TAALSKLEYGTYLAAALHFLMIRQRDATGLIGFDDTVHTLRPPRSTHGYLRELLGSLD
                     RLSRLPAEPHRTAAASVLHEVAERIGRRSLVVVITDLFENVGQHDDLLKALRHLRYRG
                     HEVLVFHVLHSETERLFEFPDVPMVFRDMETGEELTLQPSQIRENYREAVRHFSTRFR
                     SRCRERNIDFVELDTSEPYSTALLAYLNKRSRLS"
     gene            11630..13399
                     /locus_tag="AC16YU_11860"
     CDS             11630..13399
                     /locus_tag="AC16YU_11860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR6065966.1"
                     /translation="MTHRIALALAGLLSLSPVLDTRAQETDRAPVAQPAPTPTADEAP
                     DLVRTYQETITPGDLAAHLYLFASDFFEGRETAERGQKLAAAYLAAQYRKMGVQPAGT
                     VKTDDPLAPAAYFQPFPLYSSAMTRSSMTVTGADGATTTSVFSEDERDGRSYPGYGGG
                     SGLEGGVIFGGFGISDEQAGFDEMAALSASGLDLASNWLVILAGEPRDADGRPVLSED
                     GSPTRWAGSPNVKLGAFFRGDTPPLGILYVADFASDESMAAMTERAAAQLGRGGRLSL
                     DPPDGENMSARGRFPSILMISTELADAILAGTGHTVRSLASGIAGSREPNVFAVEGSR
                     VASDVSFDVAETSSENVLGFIEGSDPELKDQIVVISSHYDHVGVNEAAEGDGIFNGAD
                     DDGSGTVAVLEIAEAFARAREDGHGPRRSVLFLNVTGEEKGLLGSAYYTDREPVFPIE
                     NTVTNLNIDMIGRVDPTHPGETDEYVYIIGSNLISQELHDINLRMNSLLGTDLDLNER
                     FNSKDDPNQFYRRSDHWNFGKHQVPFIFFFTGTHEDYHGVDDEPEKILYDRMADISRL
                     IFATAWQVANQDTPPEVTGTGFN"
     gene            complement(13415..13699)
                     /locus_tag="AC16YU_11865"
     CDS             complement(13415..13699)
                     /locus_tag="AC16YU_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR6065967.1"
                     /translation="MSEHRNSNGSGDAPSLSDRVEAALDQLRPYLMADGGSVRLLDIT
                     DEMVVELELLGACGTCPMSTMTLRAGIEQALRRSVPEITRVEAVNATAGF"
     gene            complement(13750..14862)
                     /locus_tag="AC16YU_11870"
     CDS             complement(13750..14862)
                     /locus_tag="AC16YU_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR6065968.1"
                     /translation="MMDLTDRVLGALRTVVEPKSGHDIVRLGSVRELHAGEGAVRFTV
                     VVDDPVSDFARTVAERCERAVRAEIGAEAEITVSVDHPMIGLGADVSVDGSNPEGSAL
                     ARVRNIIAVASGKGGVGKSTVAVNLAVSLARAGYATGLVDTDIYGPSVPTMMGLEQAR
                     PRLNDARRMVPVEAHGVRVLSMGFLVDPDRAVIWRGPMVSSAVQQFLGETEWGELDFL
                     VMDLPPGTGDIQLTIVQTIPLAGAVVVSTPQRVALSDARKGVAMFHQVDVPVLGIVEN
                     MAWFEPPEVPGKRYYLFGQGGARSLAAELGVPFLVEIPIEEPLREACDGGVPVSVTST
                     GLAAVAFATLAEETARQVFRRNATQPLTERVEILHR"
BASE COUNT         2276 a         5329 c         5029 g         2333 t
ORIGIN      
        1 cgaactggac cgggagctcg ccgcgggacc tggactcccg ccggtgatgc gacggctcgc
       61 gaatgttggt caccgccgcg aaccggcccg agcgggcgac cgccagccag gtgccgccgt
      121 ggaggaggtc gcggccggcg agcatgcccg ccggctccgg ccagtaggca gccggcgccg
      181 tgggacgggc atggaactcg tcccgattcg ctgcgatcac gaggggtgcg tccgaacgga
      241 tgccgagggc gaccaggacc aggcacatgg gaaagggtgc gatgccaaat gtggcgatcc
      301 cgcagagaat acgggaaacg gacatatcca cgccccctgc gcgtatcttg atcgggtcca
      361 ttcgtcgacc ctccccgaaa tcccatgcga ttctcttcga ttcggctcgt ctccgcggcg
      421 ctcctcgcga tggtccttct ctgggccccc gattcccgcg cgcagaccac caggatgggc
      481 gtgcacgccg gcctgaatct cgacggcagc gaggtattca tcggcctgaa cggccagttc
      541 gggatcacga tctcggaccg gcaggccctc gtgggcctca cgggcgagct ctatcccttc
      601 atcgacaaca tgtcggtgac ggtcgtcgag ctggacgccc tcttcccgtt ccgcgtgtcg
      661 gctgtcgaac tctacgccgg tggaggcctc gcgctcagaa tgcggcggct cgatctgcca
      721 tccgactccc tcgccgatga ctcggacacc gacgtcgggc tgaacatcaa ggtcggggcc
      781 gtgttcggca acgaggaagc cggcatccgg ccgttcgtgg agctcgacca gacgatcggc
      841 gccggaacgg acctcgccgc acgcgccggc gtcttcttca tgctcggcgg tgggcggtag
      901 agcacccgca ggccccgtcc atgtcggatt cggaccatgc gcgcatcgac gtcgagcttc
      961 cctccggcgt gcgctatccg atcgtattcg gcccgctcga cgggctgccg tccggcatgg
     1021 ccaggcacgg gcttcgccag ggccggtgcg tcatcctctc ggacgagaac gtggcggcgc
     1081 tttacgggga ctccgtttcg ggggcgttct cccgggacgg gtgggacgtc cgcctcgtgg
     1141 tgctgcccgc cggggagtcc accaagtccc tcgagcattt cggcatggtg ctcgacgcgg
     1201 tgctcccgtg gggcatcgac cgcagcacgc cgctcgtcgc gctcggcggc ggcgtggtcg
     1261 gggacatcgg cggcttcgcg gcggcgagcc tgctccgggg actgcccctg gtccacgcac
     1321 ccacctcgct cctcgcccag gtggacagcg ccatcggagg caagaccgga atcaatcatc
     1381 cggccgggaa gaacctggtc ggcgccttcc accagccccg gctcgtgtgg tccgacgccg
     1441 cggtcctgac cacgctgccc cggcgtgaat ggaccggcgg cgtggccgag gtcgtgaagc
     1501 acgcgctgat cgagggcgac ccgctcctcg aacggctcga ggcacggctg gaccggttcc
     1561 ttgccgcgga ccccgccgtc gtgcccgcgc tcatccgcga agcggccgcg gtgaaggcgc
     1621 gcatcgtgga gatcgacgag cgggagtcgg gcatacgggc gttcctgaac ttcggccata
     1681 cgttcgccca cgcgctcgag cgggccctcg gctacggcac gctgacgcac ggcgaggcgg
     1741 tcgccgtggg catgagggcg gccctccacc tgtcccggag ccgccggccc acccttgcct
     1801 tcgagcgggc cgtcgccatg gtggattcga ttcccgtccc cgcgatcccc ggatccctcg
     1861 gcctggacga actcgcgggc gccatgcggc tcgacaagaa ggcggatggc cgccggcttc
     1921 ggttcgtcct tctcgacgag ctcggctcgc cctgcctggt ggatgacgtc ggcccggagg
     1981 aggtggaggc ggcgtggcga ttcgcgctcc gccggggcgc atgacgggca cctgatcgaa
     2041 tcccgctcgt tcaccagttc tgcgtccagc cgacccgacc atcccgtgtt ccagttcctg
     2101 cgccgacttc cgggccgcac cctccgcggg ctcctggcgc tggctctcgt cgcggtcgtc
     2161 gccctcgtcg ccctgacgcg aacccaggtg ggtcgcgacg cgctggcccg ccggatcgag
     2221 tccgagtttg ccgagaccta cgacggcgcg ctcacgatcg gtcgcctcac cggcaacctc
     2281 atgcgggagt tcgtcgcttc cgacatcacc gtcgtggacg gcgcgggcgg ggagtcgctc
     2341 cacatcgaca gcctgctggt caggaccagc tgggaagacc tcgtgcgcag gcggatcgtg
     2401 gtcccgcgga tcgtcctggt ccggccgtcg gtccggatcc aggtgggaga cagcggacgt
     2461 taccgggtgc ccggcttcgc gcgccgcgtt ccggcggccg attccgtgga cggcttcctg
     2521 cgtttccgct ccgcacggat ccaggtggcc gacggccggc tcgtcacgga gccgtcggac
     2581 agtgcccggg tgcgtcccga acggcccttc ttcgccgatc ccgccggtct gaggatcgag
     2641 ggcgtcgctc tggacgccct cctggactgg tcggccaccc agcgccagtt cgatctcttc
     2701 cagctgtctg cccggggcgt cacgccacag atcgaactcc ggtccgccga aacgcaggtg
     2761 atcctccagg acgaccgcat ttcgatcaat cggctccgga tcgccctgga ccgaagccgc
     2821 ctcgcggccg agggcatgat tctcctcagg gagaacgagg tcagcgaggt ggatctatcg
     2881 gtcgaggccc ccgaggtctc cttcgacgag ctgaggagcg ccatacccgg actccccctc
     2941 gccggctcgg gggcgttcgg catccgcctg agcggctcgc ccgggggtat cgcggtatcc
     3001 cggcttgagt tcgcgacgag ggagagcttt ctcgtcgcct cgggtaccgc ggtcggcctt
     3061 cccgattccc tggacttcga gatctccgcg gatcggatcc atgcccggtc gggggacctt
     3121 cgcgcgctgc tcccggacgc tgcctttctg cgaagcgctt cattcgacag cctgggcgcc
     3181 tccgggtacg cgcggggtgt cgcctgggtc acgggcgctt cggtggattc gctccgctcg
     3241 gacgggagcc tggtcctgaa cggggatgca ggctccgtgg cctcgacgtt ttccgtcgac
     3301 ctgagggacc gttcccgtcc gctggtttcg ggggacgccg agcttgacgg cctggacctg
     3361 gcggccttcc tcggcgatcg gaccacccgg ctcacgggcc gccttgaggt ggccacggag
     3421 ggcctgctcc cgcgcacgat gacgggagat gtccgggtcg aactcgcgga cagccgcatc
     3481 gggaatcgcc acgtggagca cctcgtggcg catgccagcg cggaaggaac cgcgctgcgc
     3541 ttcgatgtcg caatggacac gcaggctgcc ggctggctgc gggcggacgg actctggacg
     3601 cgtgattccc tcggaaccct ctcggccgac gcggcctgga gccggttcga cgtggctccg
     3661 ctcctgggta ccgacagcct gcacacggcg gccaccggaa ggctcggcgc gctcttcacg
     3721 ggccacgacg aggcctcgtt cgccggcgtg ctccgcctgg agatcgactc cacggtcgtc
     3781 cgcatcggcg ggcgcgagtc cctcgtcctc ccgcagcggc accggctgga cctggcgcgg
     3841 gccgacgccc ccgttccccg catccggctg agcggcgacg cgctggacgc ggaaatccac
     3901 ggtgatgtcc gcctggaggc gctctccgcc gtcgcgtcct tctggacgga tgcgatagcc
     3961 ggggcggtac gtcgcgaact cgacaagccg tatccgggca cggcgcccga ccccgcgtcc
     4021 cagctcgtcg agaacgcgcg cgacatccgg acctcggtcc tgcgcggagg tgcggagcag
     4081 gccctcgcgg acctcggcct cggacgcccc ctcgagctgc gcatcgatgc cacggtccgg
     4141 aggcccgaac tcctgacggg ctggatcccc ggcctgccga acgtccgggg gcggatccag
     4201 gtctcggccg atgcgcgggc cgacgccgat tcgctggggc tttcgatgac cctgtccggc
     4261 gatacggtcc gcgtcgacag cacgggcctg ttgaatcccc gcgcccggtt ccgggccacc
     4321 acggccttcg accaggcggt ggagcgctcc cttcggctcg acggcggcct cgtggccgat
     4381 tcgatcgcga gcggcccggt gctcctgccg tcgatcgacg tgtcgctccg gatcgccgat
     4441 gggcgcgccc gcatagccgt cgacgcgggc cgacacgacg acatcggccc gctgcacgtc
     4501 cgtgccgagg tcgagtccag gccggaccgc aaccggctcg aaatccgcga ggcgtcgctc
     4561 gacgcccgca attatgcctg gactctgagc cgcccggcga cgatcgacct gtacgccggc
     4621 ggtatcacgg tcgaggacct cttcgtcgaa agcgactact ccgacctgta cggtaaccag
     4681 tcggtggccg tccacggggc ctattccggg ctggcgaccg acaccctgtt cgtcgaggcc
     4741 gacggcgtcc ggctcgagga gctcacccgc ttcgccagcc tgaagccggc cctgggcggt
     4801 cgcctcgacg ggcaggcggc cttcacgcgc agcaatgggc ggccgctgct ggtcggcgac
     4861 gcgaccacga ccgggctcgt gctggacggc cggctcatcg gccacgtggt cgcccggagc
     4921 cggctcgtgc ccggcagccc ggaggtcagc atccgcctcg acctgacgcc cgccgacacg
     4981 gccggcttcc gttggccgca gccgccccgc gaggtgcggc ggaacgccct cacggtgacc
     5041 gggaccgtgc gcctcccggt cgcggacgaa ggggatcccg gcgagtggaa cctggatgtc
     5101 cgggcgtccc gcgcagacct cttcttcctc aagtacatct tcaacgaaac gctggaggac
     5161 gtcggcggat atctcgcggg cgaggggcgg gtgaccggct cgataagccg accggtgttc
     5221 gacgtcgact tcacggccag cgaaggcacg ttctacgtcc cggtcaccgg cgtgcgctat
     5281 acgctcgaag gcgatgtcgg cctcacccgg gacgccatcg tttttcccgg ggtgaccgtc
     5341 cgggacgccg gcggcggaag tgcgctggtc accggcagcc tgtatttcaa cgagtacgag
     5401 tacttcagct tcggcctgaa cgccgcgctc gacgagctcc agatcatcaa caccgggtat
     5461 acggacgagc tgccgttcta cggttcgatc cgcggctccg gcagcgccac gctcaccggt
     5521 cccctcgccg cggcatacct ccgcgttccg gacggtgtcg cccgggacga ctcggagctg
     5581 ttcatcccga tcgtggaatc cgcgactgcc acggacgaca gcttcatcgt cttcacggat
     5641 tccacgggcc tggtgccgga cctgcgcgag ctggcccgcc gtccgttcct cctcgcccgg
     5701 cgggccacga tggagcgccg cttcctggac ggcctcgaca tcgacatcaa cctgttcgcg
     5761 ccgcggggct ccgtcgtgca cctcgtcatc gatccgctgc tcggcgacgt gatcaacgcg
     5821 gagagcaccg ggcgtgtgca gatccggcgg gaacagggtg agttcgaaat cttcgggacg
     5881 ctcgaggtcg aaggcggcga ttacctcttc accgcgggcg aggtcttcgt ccggcggttc
     5941 atcatcgagg agggcggatc catttcatgg gagtccgatc cgatcgatgc ccggctcgac
     6001 atcccggccg tgtaccgcac gcgggcgtcg tgggccgggc tggatgccgc gaataccggc
     6061 tcgagcgcgc tgatcccgct cgtggtcgag ctccggatca cgggccgtgt catgtcaccc
     6121 gccgtggagc tctcgctggc catcgaccgc acgaaccaga acgtgctcgg ggactaccag
     6181 gcgctcgagg cgcgcctgaa caacccggag cgtgccgccg agtacgccac gagcgtgttg
     6241 ctcaccaact cgttccagct cacgaccgag accgtggact cgcggacggg tgagcagctg
     6301 gccttcaaca gcgtgtcgca gctcgtctcg gcccagctga acaggttcct caacgccgcg
     6361 ctccccaacg tcgatgtcag cttcggcctg ctcggggagc gagcgagcga cctggacatc
     6421 acgtacggcg tggccctgcg gctgctcgac gagcgcctga tcattcgggg cgagggggtg
     6481 taccagggct cccgtaccga cgcgacgaac gtcgccgccg agaccatgca gggcgaagtc
     6541 gtggtggagg tccggctgag ccccaccgtc tccgtcgagg tgttctttcg gcgcgagggg
     6601 gacgtcttcc agagtgccga gctcacgaac accacgggag ccggcctgtc ctaccagacc
     6661 gaattctcga cgtggagatc cctgttcgac cggcttttcg gctggcttac accggatccc
     6721 gagccgacgg agtctcccac gagcggaagc ggcgagctca ggtagtcgag tcggggttca
     6781 gcccgaattc ggacggattc agcccgaatt cctcgcggaa acggcgggcg aagtaggaca
     6841 ggctggaaat ccggacggcc tgcgccgcgt cgcccaccga ctgccgcccg tcctcgatca
     6901 gtgaccgggc gcgctccagg cggatcgtcc ggatgaactg gcacggcgag cagcccgccg
     6961 aatcctggat gcgtcgctgc agctgccggg gcgacaggcg aagagcccga gccaggtccg
     7021 cgaccccgaa cgaggggcga tcgagtccgt cctcgaccgc gcgccgcgcg gagcggacga
     7081 aatccgctcc ccggaggacg ggacgtgccc cgctcgccgg gcgctccacg ccgccgggga
     7141 gatccggccc ttcatggccg aaccattccg acaggccgga aaggaattcg atgcgtgaga
     7201 tgccggacat gatacctgga aacggttgaa tacgccggat ttgcgatgag caaagattgc
     7261 gccatgattc agcctgcccc cgggtatcgg ccgattcggg cgatcccaaa ctccgcccgc
     7321 tgcccgcctg cgattccgga acccgtttcg ggacctggac tcgtgggccc ggaaacacgg
     7381 ggacggcccc cggcgccgct tcacccgccg ggcgattcgc cggaggagga tcgcagcgcg
     7441 cccagcacct ggtcatggag cagcccgttg gtcgcgatca cgccccggtt catggtcagg
     7501 cggcggccgt ggccgaagtc caggggagcc ccgctgatgt cggtgacccg gccacccgct
     7561 tcctccacga gcaggcttcc cgccgcatgg tcccagacgt tctcgacgaa acgccgcccc
     7621 gagggcagtc ggaggtagat ctccgcgtcg ccgtgagcga ccacggcgta cttcgcctgg
     7681 ctgtccatgc ggatcggggg ctccgcgatc ccgagcgcct ccgccaggcg ggcggcatcg
     7741 tcgtgcgacg agtgcgtatg ctcgaccgat tcgcagtacc gggccgcgga cgcgtcggtc
     7801 gtcgcgcgca cccgcagtcg ctccgccgta ccgccgccga acagcggctc gacgtacgcc
     7861 ccttctccgc gcagcgccca cgcgaggacg ccgcacgtgt cgcccaactc caggcgcgga
     7921 caggccaagc acgcgagaac gggacgcccg tcctcgacca gggcgaggac gacggcgaac
     7981 tggtttcccc tcaggaatcc cttggtgccg tccaccgggt ccaccgtcca gtacctggag
     8041 gtgacctcgc cgcccgagcc catgtcgatc cagcgcgcga ggtcttcggc ggcggcggac
     8101 gggtggaatg cccgcacgtg cgccaggacg ctgtcggaca gcccggggtg accggcaccg
     8161 gacagttcgg ccgacgactc ctccgccacg acggtggccc ccgggaacgc gcgaccgatc
     8221 tccctgcaga tcagggcctg gctcccgtaa tcggccacgg tcacggggct cgaatcccgc
     8281 ttgcgtacgg gcatgagctc catctcgcgc agcacgttca ggcacaggcg cgccgccgcg
     8341 cgcaccgcgc cggtggccgt ttcgagctcg ggctgcaggc ggtgaggttc agccatcgcg
     8401 ggtcggggct tgtggaagct cctccatcgc gagggatccg ccgcccggca gcgtctgcca
     8461 gcccacgagc tgcaggccgt tcgtgacgac gacgaacgcg gcgcccatcg atcgggcgta
     8521 tcgaccggcc tggtccatgg cgtcctggcc cacggagacg gccggtgcct tgcactccac
     8581 gaccatccac ggccggccgt cccggccgta ggccacgatg tcgacgcggc ggcgggttcc
     8641 ctcccagacg agctccctct ccacggcgac gagtccgggc gcgaccccga cccgttcgag
     8701 aagcaggtga accgcgtgct gccgaaccca ctcctcgggc gtcagcgcca cccatttcct
     8761 gcgcacgcga tcgaagatct cggtgccgcg ctgccgccgt cgcagccgga cgtcgggcgg
     8821 ggccggcagg ttcagtttcg gcagggacac ggccatttcg gcgaagtctt cgcgcatcgc
     8881 agtcctcacc tctccgatcg ggcgggcatc acgccgcttc ccctccgtgc ccggagagca
     8941 ggtagagcac cgccatgcgg acggccaccc cgttggtcac ctggttaagg atgatggccc
     9001 gttcgtgatc gacgacttcg gacgcgagct ccacgccccg gttcaccggg cccgggtgca
     9061 tcaccaggag gtccggatgc ctctccaggt gctccatgcg gatgccgaag gtctcgtggt
     9121 actcccggag gctcgggagc agggtcttcc cctggcgctc cagctggatg cggagcgcga
     9181 tcgccacgtc ggctccctcg agcgcctcgt cgagcccgcg cacgatccgc acccgtcccc
     9241 ctgcgtccag gtgctcgatg ccggggggca tgagggtggg cggcccgcac agcgtgacgt
     9301 tcgcgcccag ccgggtaagg ccgtacaggt ccgagcggac gacccgggaa tgcgtgatgt
     9361 ccccgatgat ggagacgttc agcccgtcga gcgactcgaa ccggtccgaa atggtcagga
     9421 gatccaggag cccctgggtc ggatgctcgt gggcgccgtc cccggcattg atgaccaccg
     9481 catcgacgca ctgggtcagg aactggggtg caccgggcga cgcgtgccgg atgacgacca
     9541 tgtcaatccg catggcctcg atgttctgcg ccgtgtcctt gagcgtctcg cccttggtca
     9601 ccgacgagat cgaggccgag aagttcaccg tgtcggcgga gagccgcttc tccgcgagct
     9661 cgaacgagat ccgtgtccgc gtcgagggct cgaagaacag gttgacgacc gtgacgccgc
     9721 gaagcgtcgg cacgcgccgg atggggcggt ccaggacctc tcgaaactgc cgggcggtgt
     9781 cgagcaccag cccgatctcg tcggcggacc aggtcctgag acccagcaga tgacggtgat
     9841 gcaacgaagg acccggatcg gccggggcgg gagtgctggg tgcgttcctg gaatcgtcca
     9901 cggatcaacg ggctctcggg gtggtcacca accagacgcc ttcgcggtca tcctcctcgc
     9961 gcagccgcac gcggacctcc tcgcctggaa gcgtgggaac gtggcgaccc acgacatcgg
    10021 cccggatcgg aagctcgcgc aggccgcggt cgacgacgac caggaagtgg accgaagccg
    10081 gccgccccag gtccatcagg gcgtcgaggg cggcgcgcgc cgtgcggccg gtgtacagca
    10141 catcgtcgat gaggaaaaga tggcgctcgg acacgtcgaa cgggacgtgc gtttcgcgca
    10201 cggcgggctg tttcagccgc agccggaagt cgtcacggta catagtcgcg tccagcacgc
    10261 ccatcggaca gtcgattccg tcgaggtcgt gcatcttgcg ctgcaggcgg cgtgccaggt
    10321 gtaccccgcg cgactgcatg cccacgatgc ccatcgtctc cgacggatcc gttcccggcg
    10381 ctgcgtattc gaggatccgg cacgccatgc ggtcgagcgt gcgatcgagg tcgcgctcgt
    10441 ccatgagctg tgccttgacg acgtcctgcg gctccatggc ggcaaaggta cgtcaggcgg
    10501 tcggcgcgga gcaccccttc acgcgtattc acgcaaggcg gttcggccga ttccgcgcac
    10561 ggtggaactc ggcccgcgaa tcccgtacaa acccgcccag acccagcgag catgaacacg
    10621 caggacccgg tcttccgcta tctcgacccg acggtcgtgt cgcagctccg gaacatggag
    10681 ctgcgggcgc gcctgatcgt cgagggattc atcacgggac tgcacaagag cccataccac
    10741 ggcttttccg tggagttcgc ggagcaccgc ccctacaacc ccggcgacga gctgcgccac
    10801 gtggactgga aggtctacgc gaagaccgac cgccactacg tcaagcagta cgaggaggag
    10861 accaacctcc ggcactacgt ggtgctcgac acctcgccct cgatgcgcta ccggggcaca
    10921 gccgcgctgt cgaagctcga atacggaacc tacctcgccg cggccctgca tttcctgatg
    10981 atccgccagc gcgacgccac cgggctcatc gggttcgacg acacggtcca tacgctgcgc
    11041 cccccccgca gcacccacgg ttacctcagg gagctgctcg ggtccctgga ccggctgtcg
    11101 cgactgccgg ccgagcccca ccggaccgct gcggcctcgg tgctgcacga agtcgcagag
    11161 cgcatcggcc ggcgctcgct ggtcgtagtc atcaccgatc tcttcgagaa cgtggggcag
    11221 cacgacgacc tgctcaaggc gctccggcac ctgcgctacc gcggacacga ggtgctggtc
    11281 ttccatgtcc tgcactcgga gacggagcgc ctgttcgaat ttccggacgt ccccatggtt
    11341 ttcagggaca tggagacggg tgaagagctc accctgcagc cgtcccagat ccgcgagaac
    11401 taccgcgagg cggtgcggca cttcagcacg cgcttccgga gccgctgtcg cgagaggaac
    11461 atcgatttcg tcgaactcga tacgtcagag ccgtacagca cggcgctgct ggcgtacctg
    11521 aacaagcgga gccggctctc gtgaccgcgg accgtacaat gcatcctttt ggccgccccg
    11581 ttcgtagctt ttcgagtccc gtccaacgcc gcacctacgc agacgcccga tgacccaccg
    11641 aatcgccctc gccctcgcag gcctcctctc gctctcgccg gtcctcgaca cgcgggccca
    11701 ggagaccgac cgcgccccgg tcgcccagcc ggcgccgacg ccgaccgcgg acgaggcccc
    11761 cgacctcgtg cgcacgtacc aggagacgat cacgccgggc gacctcgccg cgcacctgta
    11821 cctgttcgcc tccgatttct tcgaggggcg ggaaaccgcc gagcgcgggc agaagctcgc
    11881 cgccgcctac ctcgcggcgc agtaccgcaa gatgggcgtc cagcccgcgg gcacggtcaa
    11941 gacggacgat ccgctcgccc ccgccgccta cttccagccc tttcccctgt actccagcgc
    12001 catgacccgt tcgtcgatga ccgtcacggg ggcggacggc gcgacgacca cgagcgtctt
    12061 ctcggaggat gagcgggacg gccgctcgta cccgggctac ggcggcggca gcggcctcga
    12121 gggcggcgtc attttcggtg gattcggcat ctcggacgag caggccggct tcgacgagat
    12181 ggccgcactg tccgcctcgg ggctggacct ggcatcgaac tggctcgtga tcctcgccgg
    12241 cgagccccgg gacgccgacg gccggccggt gctctcggaa gacggctccc ccacgcggtg
    12301 ggccggctca ccgaacgtga agctcggcgc cttcttccgg ggagacactc ctccgctggg
    12361 gatcctgtac gtcgccgact tcgcgtccga cgaatcgatg gcggccatga ccgagcgcgc
    12421 ggcggcgcag ctggggcgcg gcggccggct ctcgctcgac ccgcccgatg gcgagaacat
    12481 gtccgcccgc gggcgcttcc cgtcgatcct catgatctcg accgagctcg ccgacgcgat
    12541 cctcgctgga accggccaca cggtccggtc gctggcgtcg gggatcgcgg ggagccgcga
    12601 gcccaacgtc ttcgccgtcg aggggagccg ggtggcgagt gacgtgtcgt tcgatgtcgc
    12661 ggagacgtcg tcggagaacg tcctcggctt catcgagggc tccgatcccg agctcaagga
    12721 ccagatcgtc gtgatctcgt cgcactacga tcatgtcggg gtcaacgagg cggccgaggg
    12781 ggacgggatc ttcaacgggg ccgacgacga cggctcgggc acggtcgctg tcctcgagat
    12841 cgccgaggcc ttcgcccggg ctcgcgagga cggccacggc ccccgccggt ccgtcctgtt
    12901 cctgaacgtg accggtgagg agaagggact cctgggctcg gcttattaca cggatcgcga
    12961 gcccgttttc ccgatcgaga acacggtcac gaacctgaac atcgacatga tcggccgggt
    13021 ggatcccacg catcccgggg agacggacga atacgtgtac atcatcggct cgaacctgat
    13081 ctcccaggag ctgcacgaca tcaacctgcg gatgaacagc ctgctcggca cggacctgga
    13141 cctgaacgag cggttcaact cgaaggacga tccgaaccag ttctaccgcc gctccgacca
    13201 ctggaacttc ggcaagcacc aggtgccgtt catcttcttc ttcacgggca cgcacgagga
    13261 ttaccacggc gtcgacgacg agcccgagaa gatcctctac gatcggatgg ccgacatcag
    13321 ccggctgatc ttcgccacgg cctggcaggt ggccaaccag gacacgccgc ccgaggtcac
    13381 ggggaccggg ttcaactgag cgcgcccccc ggcctcagaa gccggcggtc gcattcaccg
    13441 cttcgacccg ggtgatttcg ggtacggatc ggcgcagggc ctgctcgatg ccggcccgaa
    13501 gggtcatcgt gctcatcggg cacgtcccgc aggcaccgag aagctccagt tccacgacca
    13561 tctcgtccgt gatgtcgagc aggcggacgg atccgccgtc ggccatgagg tagggacgaa
    13621 gctgatccag ggccgcttct acgcggtcgc tgagcgacgg ggcgtcgccg gagccgttgg
    13681 agtttcggtg ttcggacatc gatacgggat tttccacgcc gctgataacg cccgcggggg
    13741 gccttcggtt caacggtgca ggatctcgac ccgctccgtg agaggctgcg tggcattcct
    13801 gcggaagacc tgccgggcgg tctcctcggc cagcgtggcg aacgcaacag cggccaggcc
    13861 cgtcgaggtc accgacaccg gtacccctcc gtcacacgcc tctctcagtg gttcctcgat
    13921 cgggatctcg accaggaacg gcacccccag ctcggcggcc agcgatctgg ctccgccctg
    13981 tccgaacagg tagtagcgct tgcccgggac ctcggggggc tcgaaccagg ccatgttttc
    14041 gacgatgccc agtacgggaa cgtccacctg gtggaacatg gccaccccct tccgggcgtc
    14101 ggagagcgcg acgcgctgcg gcgtcgaaac gaccacggca ccggcgagcg gaatggtctg
    14161 gacgatcgtc agctggatgt cgcccgtccc ggggggcagg tccatgacca ggaaatcgag
    14221 ctcgccccac tccgtctcgc cgaggaactg ctgcacggcc gacgagacca tggggcctcg
    14281 ccagatcacc gcgcggtccg ggtccaccag gaatcccatc gaaagcacgc gcacgccgtg
    14341 cgcctcgaca ggcaccatcc ggcgggcatc gttcagccgc gggcgggcct gttcgaggcc
    14401 catcatcgtg ggcaccgacg gcccgtagat gtccgtgtcc acgagccccg tggcgtagcc
    14461 ggcccgggcc agcgagacgg ccaggttcac ggctaccgtg ctcttgccga cgcccccctt
    14521 gccggaggcg acggcaatga tgttgcgcac ccgggcgagg gcgctccctt ccggattcga
    14581 gccgtccacc gaaacgtccg cgccgaggcc gatcatcgga tggtccaccg aaacggtgat
    14641 ctccgcttcc gcgccgatct ccgcgcgcac ggcacgctcg cagcgctcgg cgaccgtccg
    14701 cgcaaaatcg gacacgggat cgtccaccac gacggtgaat cgtaccgcgc cctccccggc
    14761 atgcagctcg cgcacggatc cgaggcgcac gatgtcatgc ccggatttcg gttcgacgac
    14821 ggtgcgcagc gcgcccagga cgcggtcggt aagatccatc atgatcggga cggtcggctc
    14881 gggtggtcgt tttcaacggc ccctgacccg attgttccac cttcgctcag gcttccaaca
    14941 gcccgagctc ccgcagcgcc aggccgt
//
LOCUS       DBNHQZ010000440         5847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3245713, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000440 DBNHQZ010000000
VERSION     DBNHQZ010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3245713"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..505
                     /locus_tag="AC16YU_11875"
     CDS             224..505
                     /locus_tag="AC16YU_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR6065969.1"
                     /translation="MQLCRVTGTAVAPQKAQAFETSKLLVVQSIRLDGTVDGTPDRLA
                     LDPGYGAGPGDVVLVVREGGAVRQILERDDAPANVVIVGVVDDWEVDEG"
     gene            complement(525..1340)
                     /gene="mazG"
                     /locus_tag="AC16YU_11880"
     CDS             complement(525..1340)
                     /gene="mazG"
                     /locus_tag="AC16YU_11880"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR6065970.1"
                     /translation="MSRTKIHDPEFDEKSDAVEAYADFVGIIRHLRRDCPWDRVQTHE
                     SVKHLLIEEAYETVEAIDEENWPELRRELGDLLLHVVFHAVIAEQDGRFTMREVIEGE
                     TEKLVRRHPHVFGDTAVDGVEDVLSNWEQIKLKEKGRTSTLDGVPVQLPALLRAFRMQ
                     EKAAGVGFDFPTPDGAWSKVQEELSEFRTLVEGGAPAAEAEQELGDLLFAIVNHARLL
                     ELNPENALRRTNEKFVRRFRHIEKRLRDEGRSVADADLAEMDRYWDEAKSLER"
     gene            1653..2126
                     /locus_tag="AC16YU_11885"
     CDS             1653..2126
                     /locus_tag="AC16YU_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6065971.1"
                     /translation="MARGVNKVILIGNLGQDPELRYTGGGTAVCNLRIATNESYKDTN
                     GEWVERTEWHNVVAWARLAEICNEYLKKGSQAYFEGSLQTRSYEDKEGNTKYITEIKA
                     REMMILGGRDDSFSGGGPAAASRSRQSTPAKAGATAGGSEGGDDYTFEPDDELPF"
     gene            2362..3729
                     /locus_tag="AC16YU_11890"
     CDS             2362..3729
                     /locus_tag="AC16YU_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6065972.1"
                     /translation="MATATRGQWSGRTGFILAAAGSAIGLGNIWRFPYTAGENGGGAF
                     VLIYLVFVALVGVPVLLTELSIGRKTERNAVGAFKALVPGTWWRYVGGLGIVTGFGIL
                     AFYSVVAGWTLAYLWRSLTGEIGGALTAEQSGAIFTGLIGDPVLAVALTAAFLFLTIV
                     VVQRGVSKGIEAVTKVLMPLLFILLVILAARSLTLPGGLEGLTYFLAPDFSKITVSVV
                     MSALGQALFSLSLGMGAMITYGSYFPTKENMPQAGVSVALFDTLIAIIAGIMIFPALF
                     SVGVEPNAGPGLVFVVLPSIFGALPAGNLFAIAFYALLAIAALTSTISLLEVVVAYFV
                     DERDWKRGRAAWLMGSICFLIAVPSALSQGGVDFLTTLPGIGMDFLSVQNIVWGNYSL
                     SIGAVFITFFAGWVWGVPKALASLEASGHKLPLRGLWGFLVRYVCPIAVGLVLIFIIW
                     TQQYF"
     gene            3805..5187
                     /locus_tag="AC16YU_11895"
     CDS             3805..5187
                     /locus_tag="AC16YU_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6065973.1"
                     /translation="MEADPPHALPALVDAATQAVEVDPVTFVLGGAVLLLLLLVSALV
                     SGSEVALFSIDATTREHLATAGDRASHRVLRLLERPRALLVSILILNTVVNVGAAVLA
                     AVLTHRLAALYGWSEALTFVGEVIALTFVLLVVSEITPKLLASRNAVGFARRVSLPLS
                     IAHRPLFPLSAAIARRMQAFHGRFAASSTRLSGEDVKAMAEIGEAHGSLEEEERELIH
                     SIVEFGETTVREIMISRLDIVAIPAGATLSDAVATIRKSGHSRMPLYVEHLDNILGVV
                     YAKDLLRYMVSHAREEHIDWTRITRPPMFVPLGKKLDDLLRDFQSRKTHIAIVVDEYG
                     GTAGLVTLENVLEEIVGDIRDEHDEFEEALFEKLDDERYRFDARIDLDELNELLGTEI
                     DTEEFDFETLGGLIFHLTGAIPSVGDEVSYGDLHMTVETVLHHRIGRVLLERRPDRER
                     PATVQDDPVP"
     gene            5358..>5847
                     /locus_tag="AC16YU_11900"
     CDS             5358..>5847
                     /locus_tag="AC16YU_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6065974.1"
                     /translation="MRQIKEYCGIVGVYNAPNASHLIYLGLHALQHRGQESAGIATST
                     YDEARNKQVMPAHKDAGLVLDVFRDPAIFETKLLGSAGIGHNRYSTTGSSDNLANIQP
                     LVVNYRDGNLALAHNGNLSNARELRSSFSTRGTIFQTTSDSELILHLVAQSPRRRQID
                     KVL"
BASE COUNT          905 a         2014 c         1905 g         1023 t
ORIGIN      
        1 gagcggagct cggtgatcgt ggcgactgct tcgatgccgc gggtgctgta catggattga
       61 ccggcttgtt gacggacgcg gagtgcgtaa tccctcccat cttagacacc gcccgtcgca
      121 attcataata caattcctgc acgaatcacg ccgcggcgca tgccttggca cgagcgcctg
      181 tccggggtac attgcgcacg gagtaccgga acgggatctg accatgcagc tctgccgagt
      241 gacgggcacc gcggtagcac cccagaaggc ccaggccttc gagacgagca aactcctcgt
      301 ggtgcagtcc atccgcctcg acggaaccgt cgacggaacg ccggaccggc tggcgctcga
      361 tcccggctac ggcgccggac ccggcgacgt ggtcctggtg gtccgggagg gtggggccgt
      421 ccggcagatc ctggagcggg acgacgcgcc ggccaacgtc gtgatcgtgg gcgtcgtcga
      481 cgactgggag gtggacgaag gctgatccgc gatgcgactc gggatcagcg ctcgagcgac
      541 ttcgcctcgt cccagtagcg gtccatttcg gcgagatcgg cgtccgccac ggagcgtccc
      601 tcgtcgcgga gccgcttctc gatatgccgg aatcgacgca cgaatttctc gttggtgcgg
      661 cgcagcgcgt tctccgggtt gagctcgagc aggcgggcat ggttgacgat cgcgaagagc
      721 aggtccccga gctcctgctc ggcctctgcg gccggtgccc cgccctcgac gagcgtgcgg
      781 aattccgaga gctcctcctg caccttgctc cacgccccgt cgggggtggg aaagtcgaag
      841 ccgacgcctg ccgccttctc ctgcatccgg aaggcccgga gcagggccgg cagctggacc
      901 ggcacgccgt cgagcgtgga ggtcctcccc ttctccttca gcttgatctg ttcccagttc
      961 gagagcacgt cctccacccc gtcgacggcc gtgtcgccga acacgtgggg gtgacggcgg
     1021 accagtttct cggtctcgcc ctcgatcacc tccctcatcg tgaaccgtcc gtcctgctcc
     1081 gcgatcacgg cgtggaagac cacgtgcagg agcaggtcgc cgagctccct gcgcagctcg
     1141 ggccagttct cctcgtcgat cgcctcgacg gtctcgtacg cctcctcgat caggagatgc
     1201 ttcaccgatt cgtgcgtctg gacgcggtcc cacggacagt cgcgccgcag gtgccggatg
     1261 atgcccacga aatcggcgta agcctcgacg gcatcggatt tctcgtcgaa ctcggggtcg
     1321 tggatcttcg tccgggacat ggcggggcgg gcggcggagg agcgttctca acgtcccccg
     1381 ccgcgaaagg tttggggcga atccggggcc gcccgggcgg ttctgcaagt tgtccacagt
     1441 gacggcacgt cgtcacccga ctggcgtata attcctgtcc gttacgccgc gcacggtgcg
     1501 gccctccata gggcgcacgg cgcgggtctg gacggaccag tgcttgtgca tctccctggg
     1561 aatcgtgcgg gcaggccggg caaccggtgg actcatgtag aatcgccagg gaccaatcaa
     1621 cacatcagga caacgcgaca ggaggtgtaa tcatggcacg tggcgtcaac aaggtcatcc
     1681 tcatcggcaa cctggggcag gaccccgagc tccggtacac cggtggcggc accgcggtgt
     1741 gcaatctccg gatcgccacc aacgagtcgt acaaggacac gaacggcgag tgggtcgagc
     1801 ggacggagtg gcacaacgtg gtcgcctggg cccggctcgc cgagatctgc aacgagtacc
     1861 tgaagaaggg ctcccaggcg tacttcgagg gctccctgca gacccgctcg tacgaggaca
     1921 aggagggcaa cacgaagtac atcaccgaga tcaaggcccg ggagatgatg atcctcggcg
     1981 gccgcgacga ctcgttctcc ggcggcggac ccgccgcggc ttcgaggagc cggcagtcga
     2041 cgccggccaa agcgggggcg acggccggtg gttcagaggg aggcgacgac tacacgttcg
     2101 agccggacga cgagctgccg ttctagcccg cgggatcctc gcggagaccg ggattccggg
     2161 ccggtggacg acaccggcgg atcgactcgg cgggccccgg cgatcgaggg gccgccgtgc
     2221 cgccagacct ggccggggcc atggcggcgg ggccgggttc tccgccgggc ggggccgctt
     2281 gcgcggggct aaggcgtacc gttatccttg ggcctttcac gggcgcggca cgccctaccc
     2341 acggaatcaa catcgcgatc catggctacc gccactcggg gtcagtggtc cggcaggacc
     2401 ggcttcatcc ttgcagccgc cggctcggcg atcggcctcg gcaacatctg gcgattcccg
     2461 tacacggcgg gtgagaacgg cggcggcgca ttcgtcctca tctacctggt cttcgtggcg
     2521 ctggtcggcg tgccggtgct tctgaccgag ctttcgatcg gccggaagac cgagcggaac
     2581 gcggtgggcg ccttcaaggc cctggtgccg ggcacctggt ggcgctacgt gggcggactc
     2641 ggcatcgtga ccggcttcgg catcctcgcc ttctactccg tggtcgccgg ctggacgctc
     2701 gcgtacctgt ggagatcgct gaccggggag atcggcggcg cgctcacggc cgagcagagc
     2761 ggagccatct tcacgggact catcggcgat cccgttctcg ccgtcgcgct cacggcggcg
     2821 ttcctgttcc tgacgatcgt cgtcgttcag cggggggtct cgaaaggcat cgaggccgtg
     2881 acgaaggtgc tgatgccgct cctctttatc ctgctggtga tccttgcggc ccgttcgctc
     2941 acgctgcccg gcggattgga gggcctcacc tatttcctgg cgcccgactt ctccaagatc
     3001 accgtcagcg tggtcatgag cgccctcggc caggcgctct tcagcctgtc gctgggcatg
     3061 ggcgccatga tcacgtacgg gtcgtacttc cccacgaagg agaacatgcc gcaggcgggt
     3121 gtttcggtcg cgctgttcga cacgctgatc gccatcatcg ccggcatcat gatcttcccg
     3181 gcgctcttct ccgtgggcgt cgagccgaat gccgggccgg gtctggtctt cgtcgtgctg
     3241 ccgtcgatct tcggggccct tccggcgggc aacctgttcg ccattgcctt ctacgccctg
     3301 ctcgccattg ccgcgctcac gtccacgatc tcgctgctcg aggtagtggt cgcctatttc
     3361 gtggacgaac gggactggaa gcggggtcgg gcagcctggc tcatgggatc gatctgcttc
     3421 ctgatcgccg tgccgtccgc gctttcccag ggcggcgtcg actttctgac caccctgccg
     3481 ggcatcggca tggacttcct gtcggtccag aacatcgtct ggggcaacta ctcgctctcg
     3541 atcggcgccg tgttcattac gtttttcgcc ggatgggtgt ggggcgtgcc caaggcactc
     3601 gcttcgctcg aggcgtcggg gcacaagctt ccccttcgcg gcctttgggg cttcctggtc
     3661 cggtacgtct gccccatcgc ggtcgggctc gtgctgatct tcatcatctg gacgcagcag
     3721 tatttctgat gcgggcgccg cgaccccggt accgtatctt ttcgcacgac cgttccacat
     3781 caaggcctcg tcccgccgcc gttcttggaa gctgaccctc cccacgcgct accggcgctg
     3841 gtggacgccg ccacgcaggc cgtcgaggtc gatcccgtca cgttcgtgct cggaggagcc
     3901 gtgctgctcc tcctgctgct cgtctccgcg ctggtctcgg gatcggaggt ggccctcttc
     3961 tccatcgacg ccacgacgcg cgagcacctg gccacggccg gcgaccgcgc cagccaccgg
     4021 gtgctccggc tcctcgagcg cccgcgcgca ctcctggtct cgatcctgat cctgaatacg
     4081 gtcgtgaacg tcggcgccgc cgtactggcg gccgtcctca cgcaccggct cgccgccctc
     4141 tacggatgga gcgaagccct caccttcgtg ggcgaggtga tcgcgctgac cttcgtgctg
     4201 ctggtcgtga gcgagatcac gcccaagctc cttgccagcc ggaacgccgt cggcttcgca
     4261 cgccgcgtgt cgctgcccct gtccatcgcc catcggccgc tgtttccgct ctcggcggcg
     4321 atcgcccggc gcatgcaggc cttccacggg cgcttcgccg catcgtccac gcggctgtcc
     4381 ggggaggacg taaaggccat ggccgagatc ggtgaagccc acgggtcact cgaggaggag
     4441 gagcgcgagc tcatccactc catcgtggag ttcggcgaga ccaccgtgcg cgagatcatg
     4501 atcagccggc tggacatcgt cgccatcccg gcaggggcca ccctgtcgga cgcggtcgcc
     4561 accatccgga agagcggcca ctcgcggatg cccctgtacg tggagcacct ggacaacatt
     4621 ctcggtgtcg tctacgccaa ggacctgctc cgctacatgg tttcgcacgc ccgcgaggag
     4681 catatcgact ggacgcggat cacccggccg cccatgttcg tgccgctcgg caagaagctg
     4741 gacgacctcc tgcgggattt ccagtcccgg aagacccata tcgcgatcgt cgtggacgag
     4801 tacggcggga cggccggcct ggtcacgctc gagaacgtgc tcgaggaaat cgtaggcgac
     4861 atccgtgacg agcacgacga gttcgaggag gcgctcttcg agaagctcga cgacgagcgc
     4921 taccggttcg acgcgcgaat cgatctcgac gaactgaacg agctcctcgg gaccgaaatc
     4981 gacacggagg aattcgactt cgagacgctg ggcggcctca tcttccacct gaccggcgcc
     5041 attccctcgg tcggcgacga ggtctcgtac ggcgacctgc acatgaccgt cgaaaccgtc
     5101 ctccaccacc ggatcggccg agtcctcctg gagcggcgcc cggaccggga gcgtccggcc
     5161 accgtgcagg acgacccggt cccatgaacc cggcccgtgc gcctggtcgc gcccgccgga
     5221 cggcttcctc cgcggcgccc ggtgcgtgaa accggccacc ccggtttcgc attcaacccg
     5281 tttccgggac accttccgaa ccccccgggt cccgttcggg cgacatggcc cggcacccgg
     5341 ccgaccagcg cctccgaatg cgccagatca aggagtactg cggcatcgtc ggcgtctaca
     5401 atgcgcccaa tgcttcgcat ctcatctacc tgggcctgca tgcgctccag catcgcggcc
     5461 aggaatcggc gggcatagcg acgtccacgt acgacgaggc gcgcaacaag caggtcatgc
     5521 cggcgcacaa ggatgccggc ctcgtcctgg acgtgttccg ggatcccgcg atcttcgaga
     5581 cgaaacttct cggaagcgcc ggaatcggcc acaaccggta ctcgaccacg ggttcctcgg
     5641 acaacctggc caacatccag ccgctcgtgg tgaactaccg cgacggcaac ctggccctgg
     5701 cccacaacgg caacctctcg aatgcccggg agctccggtc cagcttcagc acccgcggca
     5761 cgatcttcca gaccacgagc gattcggagc tcatcctgca cctggtcgcc cagagtcctc
     5821 gccgacggca gatcgacaag gtgctcg
//
LOCUS       DBNHQZ010000441         4708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_536650, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000441 DBNHQZ010000000
VERSION     DBNHQZ010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_536650"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="AC16YU_11905"
     CDS             <1..748
                     /locus_tag="AC16YU_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR6065975.1"
                     /translation="DFDLYIYRDAYSVIPAQNPANYYGVTRGSTYLSSVNDGDWALYA
                     GVEFGSETDPPNGYDYQRTPGTVEVMASSATAGGTVELWIDSLDTGTRIAQVAVQGTG
                     SWTSFDTFVAGVDSISGRHDLYVRFSGSGGGELLRMQSLRFRPRRVPVTIGTHRNEAP
                     PKGWSLDRNFPNPAGSTTTIAFDLPRPSHVRLALYDVLGRRLDELVSGPRPAGRHEVS
                     LDTSRLPTGSYVYALDAGGLRLTQTMAVVH"
     gene            complement(755..1648)
                     /locus_tag="AC16YU_11910"
     CDS             complement(755..1648)
                     /locus_tag="AC16YU_11910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6065976.1"
                     /translation="MKSLTLVLILSMAVTPAISNESSAQDRPPWGFGREIVLNEDDVA
                     AFPEPPDGIVDFREDIPHGRLEMIEYESKTVGTTRRMNVYTPPGYTADRAYPVLYLLH
                     GIGGDETEWARFATPDVLFDNLIADGQAVPMIVVMPNGRAQENDRAEGNVFASAPAFA
                     AFERDLLDDVIPTIEARYGVLADREHRALAGLSMGGGQSLDFGLKHLDTFAWIGGFSS
                     APNTYPPAELIPDPAAAKEQIELLFLSCGNKDGLIGISQGVHAYLEEHGVPHVWHVDG
                     NGHDPTHWRNSLYHFAQLLFR"
     gene            complement(1673..4066)
                     /locus_tag="AC16YU_11915"
     CDS             complement(1673..4066)
                     /locus_tag="AC16YU_11915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HIR6065977.1"
                     /translation="MTLWYDRPAAIWEEALPLGNGRLGAMMFGGVGTEILQLNEETVW
                     AGEPGNNILPGLRERLHEIRRLIFEGRHQDAQRLADAVQPWDTGLDTNYGMSYQTVGD
                     LVLAFDDTSGVLGYRRDLDISRAVATVRYDRDGVRFTREAVASLADDVIAVDLTADRP
                     GAISLSLGMRSPHERHTIRTGTDELVLTGTSGNLENKAGRVQFVTIARPRVEGGRLEA
                     TDSTLVISGADRVTLFVSIGTNVRSYRDLSGDAEERARTALDGAYAKDFDVLKRAHVA
                     AYGSLFDRVHLDLGVTDSVRKPVDVRLAEFGRGHDPHLVALTFQFGRYLLISSSQPGG
                     QPATLQGIWNRDLYAPWDSKYTVNINTEMNYWPAEVTHLPEMHEPLFDLIRDVSETGR
                     EAARGLYGARGWTIHHNTDLWRISGVVDGAFYGLWPMGGPWLSQHLWYHYVYGGDAGF
                     LRDAYPALKGVSLFLKDVLQEDPETGWLVVCPSMSPENAHHPGTSIACGTTMDNQLVH
                     DVFSNVIEASALLGLDRAYADSLSAIVARLPPMQVGRWGQLQEWMHDWDRPDDTHRHV
                     SHLYGLYPSNQVSPHRTPELFSAARTSLVARGDASTGWSMGWKVNLWARLLDGDHALK
                     LISDQLTPAIQPDGTQRGGTYPNLFDSHPPFQIDGNLGFTSGVAEMLLQSHDGAVHLL
                     PALPGAWPQGEVTGLRARGGFVVDLRWKDGALTTATVHSTLGGNCRIRSYVPLAGDGL
                     TEARGSNSNRFFAVPNTAAPLIHAETPVEAFVPPTSYLYDVSTEPGDVIEVTRLGVP"
     gene            complement(4426..>4708)
                     /locus_tag="AC16YU_11920"
     CDS             complement(4426..>4708)
                     /locus_tag="AC16YU_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR6065978.1"
                     /translation="ELETSVMMHVSPGLVRPLGEAGEGRAKRFRVRGLREGLAWAPRD
                     WAQVTGDTGVGDPSAAAAEKGRRFFEAVTRVLSTFIVDLAGADLDDLYE"
BASE COUNT          770 a         1591 c         1551 g          796 t
ORIGIN      
        1 cgacttcgac ctgtacatct accgcgacgc ctattcggtc attccggcgc agaatcccgc
       61 caactactac ggcgtgaccc ggggcagcac gtacctgtcc agcgtgaacg acggggactg
      121 ggccctctac gccggcgtcg agttcggatc ggagaccgat cccccgaacg gctacgacta
      181 ccagcgaacg ccgggaaccg tcgaggtgat ggcatcgagc gcgaccgcgg gcggcacggt
      241 ggaactctgg atcgactcgc tggacacggg cacacgcatc gcccaggtgg ccgtgcaggg
      301 cacgggcagt tggacctcct tcgacacgtt cgtcgccggg gtcgattcca tctccggccg
      361 acacgacctg tacgtccggt tctcgggctc cgggggcggc gagctgctgc ggatgcagag
      421 tctgcgtttc cggcccagac gagttcccgt cacgatcggc acgcatcgaa acgaagcgcc
      481 gccaaagggc tggagtctcg atcggaactt ccccaacccc gccggatcca ccacgacgat
      541 cgcctttgac cttccccggc cttcgcatgt caggctggcc ctgtacgacg tcctcgggcg
      601 gcgcctcgac gagctcgtga gcggtccgcg accggccggc cggcacgagg tctcgctgga
      661 cacgtcacgc cttcccacgg gctcctacgt ctatgcgctg gatgccggcg gtctgcggct
      721 cacgcagacc atggccgtcg tgcactgacc ccgctcatct gaagagcagt tgagcgaagt
      781 ggtagaggct gttccgccag tgtgtcgggt cgtgcccgtt gccgtccaca tgccagacgt
      841 gcggcacccc gtgctcctcc aggtaggcat gcacgccctg gctgatgccg atcagtccgt
      901 ccttgttgcc acacgacagg aacagcaact cgatctgctc cttcgctgcc gcaggatccg
      961 ggatcagctc ggcgggtggg taagtgttcg gcgccgacga gaagccaccg atccaggcga
     1021 aggtgtcgag gtgcttcagg ccgaaatcaa gggattggcc tccccccatc gaaaggccgg
     1081 cgagggcgcg gtgctcgcgg tcggcgagca cgccgtagcg cgcctcgatc gtcggaatga
     1141 cgtcatcgag aaggtcgcgc tcgaacgccg cgaaggcagg cgccgaggca aagacgttgc
     1201 cctcggcgcg gtcgttctcc tgggcccgcc cgttcggcat gaccacgatc atggggacag
     1261 cctggccgtc ggcgatcagg ttgtcgaaga ggacgtcggg cgtggcgaag cgcgcccact
     1321 ccgtctcgtc gccgccgatg ccgtgcagca ggtagaggac cgggtacgca cggtcggcag
     1381 tgtatcccgg gggcgtgtag acattcatcc tgcgcgtcgt acccacggtc ttcgattcgt
     1441 actcgatcat ctcgagcctg ccgtgcggaa tgtcctcgcg gaaatcgacg atgccgtccg
     1501 gcggctcggg gaaggcggcg acatcgtctt cgttgagcac gatttcccgt ccgaatcccc
     1561 acggcgggcg atcctgggct gacgattcgt tgctgatcgc aggcgtcacg gccatggaga
     1621 ggatgagtac gagcgtgagg gacttcatcg gaggatcgga gatattggcg tatcagggca
     1681 caccgagccg ggtcacctcg atgacgtcgc ccggctccgt tgacacgtcg tacaggtacg
     1741 atgtgggtgg cacgaatgcc tcgacaggtg tttccgcatg aatcaagggc gccgcggtgt
     1801 tcggcaccgc gaagaaacgg ttcgaattcg atccgcgggc ttccgtcagg ccgtccccgg
     1861 ccagtgggac gtacgatcgg atcctgcagt tgccgccgag cgtcgagtgg acggtcgcgg
     1921 tcgtcagcgc cccgtccttc caacgcagat cgaccacgaa tccgccccgg gcgcgcagac
     1981 cggtcacctc tccctgtggc cacgcccccg gcagagccgg cagcaggtgg acggcgccat
     2041 cgtggctctg gagcagcatt tccgcgacgc ccgaagtgaa gccgagattg ccgtcgatct
     2101 ggaagggcgg atgagagtcg aagaggttcg ggtaggtgcc gccgcgctgg gtgccgtcgg
     2161 gctggatggc aggggtgagc tggtccgaga tcagcttcag ggcatggtcg ccgtcgagca
     2221 gccttgccca gaggttcacc ttccatccca tcgaccagcc cgtggaggcg tcgccgcggg
     2281 caacgagcga ggtccgggcg gccgagaaca gttccggcgt gcggtgcggt gacacctggt
     2341 tcgacgggta cagtccgtag aggtgcgaca cgtggcgatg ggtatcgtcc ggccggtccc
     2401 agtcgtgcat ccactcctgg agctgccccc accgcccgac ctgcatcggc ggcagccggg
     2461 ccacgatcgc ggagaggctg tcggcgtacg ccctgtccag gccgagcagg gccgatgcct
     2521 cgatgacgtt cgaaaacacg tcgtgcacga gctggttgtc catcgtggtt ccgcaggcga
     2581 tgctcgtgcc cggatgatgg gcgttctcgg gcgacatgga cgggcagacc acgagccatc
     2641 ccgtctccgg gtcctcctga aggacatctt tcaggaagag cgacacccct ttgagcgcgg
     2701 gatacgcgtc ccgcaggaac ccggcgtcgc cgccgtacac gtaatggtac cacaggtgct
     2761 ggctgagcca cgggccgccc atgggccaca ggccgtagaa ggcgccgtcc accacgccgg
     2821 atatgcgcca gagatcggtg ttgtggtgga tcgtccagcc cctcgccccg tacagcccgc
     2881 gcgccgcttc ccgcccggtc tccgaaacgt cccgtatgag atcgaacaac ggctcgtgca
     2941 tctccggcag atgggtgacc tccgccggcc agtagttcat ctcggtgttg atgttcaccg
     3001 tgtacttgct gtcccacggg gcgtacagat cgcgattcca gataccctgc agggtcgctg
     3061 gctggcctcc cggctgggaa ctcgagatca gcaggtaccg gccgaactga aaggtgagcg
     3121 cgaccaggtg cgggtcgtgg ccccggccga actccgccag tcgcacgtcg acgggtttgc
     3181 gcacggaatc cgtgacgccg agatcgagat gcacccggtc gaacagcgag ccgtaggccg
     3241 ccacatgggc ccgcttcagc acgtcgaagt ccttggcgta cgcaccgtcc agcgcggttc
     3301 gcgccctctc ctccgcatcg cccgacaggt cccggtagct ccggacgttc gttccgatcg
     3361 acacgaacag ggtcacccgg tcggcgcccg agatcacgag cgtgctgtcg gtggcctcga
     3421 gccgaccgcc ctccaccctc ggcctggcga tcgtgacgaa ttgcacacgt cccgccttgt
     3481 tctcgaggtt gcctgaggta ccggtcagca ccaattcgtc cgtcccggtt cgaatcgtgt
     3541 gtcgctcgtg agggcttcgc attccgagcg agagggagat cgcgcccggc cggtcagcgg
     3601 tcaggtcgac ggcgatgacg tcgtccgcga gcgacgcgac ggcctcgcgg gtgaatcgga
     3661 ccccgtcccg gtcgtagcgg actgtcgcaa cggcccggga gatgtccagg tcgcgtcgat
     3721 agcccagaac tcccgacgta tcatcgaacg cgagcaccag gtccccgacc gtctggtagc
     3781 tcattccgta gttggtgtcc agtccggtat cccagggctg aacggcgtcg gccagtcgct
     3841 gggcatcctg gtgacgtccc tcgaagatca ggcgacggat ctcgtgcaga cgttcgcgca
     3901 gcccgggcag aatattgttt ccgggctccc ctgcccagac cgtctcctcg ttcagctgca
     3961 ggatctcggt cccgacgccg ccgaacatca tggcgcccag gcgcccgttg ccgagcggca
     4021 atgcctcctc ccatatcgcg gcaggtcggt cgtaccacag cgtcattgtc ccggaagacg
     4081 ggtgtgatcc ggacttcgtg caacccgaaa gagccgccgc agacagaatc agccgggcaa
     4141 tgcgtgcgcc ggtccggttg cgcatcccga atgggccggt cccccggtga tgtcgatcgg
     4201 cttccatcgc ttgcacggcc aacgcggatt caatggcccc ccacgaatcg gtgcgtgagc
     4261 atgatccgct ttcccgaaat cgtgggtgat gtcccgatca tcatgggtcg gcaccggcgg
     4321 ccattgcatg gatccctcaa tatggaccct gccggcggcg aaatccagcg tgccgatggc
     4381 ccacgccggg cgcgtattga cctggcgtac ccgtctccct gcggctcact cgtacaggtc
     4441 atcgaggtcc gcgccggcga ggtcgacgat gaaggtcgag agcacccggg tgacggcctc
     4501 gaagaaccgg cggcccttct ccgccgccgc cgccgacgga tcgccgacgc ccgtgtcccc
     4561 ggttacctgg gcccagtcgc gcggggccca ggcaaggccc tcccggaggc cccgcaccct
     4621 gaaccgcttc gcgcgcccct cgccggcctc gccgagcgga cggaccaggc cgggactcac
     4681 gtgcatcatc acgctcgtct cgagctcg
//
LOCUS       DBNHQZ010000442         7684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3789140, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000442 DBNHQZ010000000
VERSION     DBNHQZ010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3789140"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="AC16YU_11925"
     CDS             <1..1252
                     /locus_tag="AC16YU_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6065979.1"
                     /translation="AVDVSLSMLVDRGAEWKQIFDEAWRYQRDYFYDPGMHGNDWDAV
                     YRRYAPLVPHVRHRADLNYVLDQTNGELSVGHSFVFGGDMPDVDSPKTGLLGADLEAH
                     DGAWRIARIFTSESWNPGLSAPLDQPGLDVSEGDYVVGVNGQRLTASMDPYRLLDGTS
                     GLQTVLHVNGRPSMDGARTVTVEPIGGETGLRQRAWVEDNRRKVDELSGGRVAYAWIP
                     NTGGGGVVSFDRYFFAQQDRQAAVIDERFNGGGLLDDYMVDLMTRTLRAAITNEVPGG
                     RPFRLPAGILGPKVLLINEMAGSGGDFFPWVFRHQEAGPLIGKRTWGGLVKSSVHYAL
                     IDGGALTAPDNAVFDPVEGRFIAENEGVPPDIEVEIDARSAADGRDPQLERAVEEALR
                     LLDEAELPDVRAPSFPRPSKRPGN"
     gene            1261..1758
                     /locus_tag="AC16YU_11930"
     CDS             1261..1758
                     /locus_tag="AC16YU_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR6065980.1"
                     /translation="MADLFERLAALGVAFDRVDHPPVFTVDEATRLVPPLHGARTKNL
                     FVRDKSGRRHYLVTVGHGKAVDLKALARTVGVPSLSFGSPDRLKRHLGIEPGSVSLLA
                     VVNDTAGAVEVIVDRELWASERIRCHPLVNTVTLSILRADLERFLDDTGHRPRVMDVP
                     GREDG"
     gene            1755..2207
                     /locus_tag="AC16YU_11935"
     CDS             1755..2207
                     /locus_tag="AC16YU_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6065981.1"
                     /translation="MSAHIRHTASFRVHRPVDVVFPLFSPEGERLWVPGWNYVDVMGT
                     SELSEDYVFLTESHDHASGQAVWIVKRFEPGRHLVAFYKVEPGDKVGVVTVRCREEAP
                     GVTEVEVTYAYRALSDRGLAFVERFTAEAYEAYIGEWKELLDRYFGKG"
     gene            complement(2252..5032)
                     /locus_tag="AC16YU_11940"
     CDS             complement(2252..5032)
                     /locus_tag="AC16YU_11940"
                     /inference="COORDINATES: protein motif:HMM:NF026255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HIR6065982.1"
                     /translation="MRPTLLRLRGVLLAAALLLATDVAAQDSRDVRGVVLDTAGVGLR
                     GATVVALSKPDSVIVKFAIADRDGSFTLRRVPAGEYVLQVTFVGYSTWSREVTLSGED
                     LDVGTLVLTELVSELDELVISSDHVPFVVKKDTLEYNARAFAVRPNATVEDLLKRLPG
                     IEVADDGSIKAQGEDVKKVLVEGKEFFGSDPTVATRNLPAEAVDKVQVYDKLSDMAEF
                     TGIRDGDEEKTLNLDLTDDAQQGYFGNVAGGYGDPERYDGQASVNRFTPRTQLSLLAN
                     VNNINRQGFSFQDYIRFMGGMQTLMSDGMRPGGIQLGTDISDGFSETMAFGVNASHDF
                     GAKTSVRSSWFMSSIDNRQDRTVNQEELLGSSVSSLVNESSGQTSDNVTHRVNLNVDH
                     KLQDGHDLRFRGNLQASGSTLLNQGGRTTRTATGLTQNTNVATYASDGDDLGGDASLT
                     WRRRLSERGRSLVAEARVNLNDSDVSSDLESVTGLYEEGDLVTYDEIVQQQSRLGNTL
                     SQTQRLSLTEPLGAGRQLEFSAEHNRVDEDQDKRIYDLVSGEPVFNDALSSGFDRTHT
                     FWRGGSRFRHNDERLSLGAGLEVQRSQLDGRILDRDEDVGSGYTHLLPSASIGWQFRQ
                     GMNLDARYRTSTREPSMTELSPVVDNSDPLNVFVGNPDLTPEYRHTLNLHFMLFDQFT
                     FTNLFSMVAVTHTRNKIVRSRTVDDQFRQTVTSVNSGDDWTVNGRVDFGTPIRPIGAK
                     ISLSNSTLFSRGSEFVNQVQNSTDILRSTVEARLENRTKDLFDVRGGARYTFNRIGYS
                     LNRALNQDYVNRTYFADATWYPHPSWTLSASLDYRTYAQDVFGENRDVALVEATVAKT
                     FLDDRVEIRLTGLDLLDQNEGVDFSNSATRIREERIESLGRYVMLKFVYHLSAFGRGR
                     NRGMTVDVRRHD"
     gene            complement(5079..5831)
                     /locus_tag="AC16YU_11945"
     CDS             complement(5079..5831)
                     /locus_tag="AC16YU_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HIR6065983.1"
                     /translation="MRRPLLLVAIALLTLPARAQEGVVTYDESVKIEIELPPEMAQMR
                     DQIPNSSTSTKLLYFSEAGTLMKNAPKKDGEESGVRVGGEGMMIRMMTSGDVDNQVYT
                     DLEGGRVIEKRDFLGRTFLVTGEPEAYAWRLTGEQGEFLGYACMKAVATRDSTTVEAW
                     FTPEIPVPGGPGPYGGLPGLILAVNVDDGRMSITAKEVSLGALDEGVLTPPEGGREVT
                     RDEFRAIVDEKMKEMGAQRGREGATFHVRIGN"
     gene            5999..6472
                     /locus_tag="AC16YU_11950"
     CDS             5999..6472
                     /locus_tag="AC16YU_11950"
                     /inference="COORDINATES: protein motif:HMM:NF039391.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HIR6065984.1"
                     /translation="MARPFATLLLLLSVSPAAGQSAGVDGLAWLQGCWIQVDGERTIE
                     EHWMTPRGDAMVGMSRTSRSGRLRAYELVVIHSDSSGLTYTAHPSGQPGAAFRSTSVG
                     DGTVAFENPDHDFPRRIVYERAGADSLLAWIEGPPGGEPRRTTFRYGRTPCPGPG"
     gene            6474..6974
                     /locus_tag="AC16YU_11955"
     CDS             6474..6974
                     /locus_tag="AC16YU_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cif family virulence factor"
                     /protein_id="HIR6065985.1"
                     /translation="MNLALRPSPILALALVATACGAPSPQEFDEQRLASVEADVRAFV
                     SSIPIHLAERGPTAWLDHFDRGPDFFMASDGVEVFPDHATADELVRGFAPSIAAMEIT
                     WDSVRVQVLTPDWAVFSTAYRETLTDTSGVDITFGGYVTATVVRRAAGWRIQNLHWSS
                     PAPGGS"
     gene            6991..>7684
                     /locus_tag="AC16YU_11960"
     CDS             6991..>7684
                     /locus_tag="AC16YU_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6065986.1"
                     /translation="MREMSLKTTAFLALLVSAAACEPEAPDPMRAATAWGSPAWEVRL
                     GDSTAHWIGLHALDRNVVWAAGRGGRVARTTDGGATWTVVTVPGADSLQFRDAHAFSA
                     DEAFVLSIGNGPDSRIYATRDGGATWTDVSDNVPDLGHGMVVSRLEPSHHVAGRVYLS
                     FDGHYSDDYAPYVHVSEDYGASWRRITGGLPDWSVNVVKEHPRTPELLFAGNEVGVYA
                     SFDRGGAWHRLPG"
BASE COUNT         1113 a         2658 c         2660 g         1253 t
ORIGIN      
        1 cgcggtcgac gtctccctga gcatgctcgt ggaccgcggt gccgagtgga agcagatctt
       61 cgacgaggcc tggcgctacc agcgggacta cttctacgac ccgggcatgc acggcaacga
      121 ctgggacgcg gtgtaccggc ggtatgcgcc cctcgtgccg cacgtgcggc accgggccga
      181 cctcaactac gtgctggacc agacgaacgg cgagctgtcc gtcggccact cgttcgtctt
      241 cggcggggac atgccggacg tcgattcgcc gaagaccggg ctcctgggcg ccgacctgga
      301 ggcccatgac ggcgcctggc gcatcgcgcg catcttcacc tccgagagct ggaatccggg
      361 cctttcggcc ccgctcgacc agccgggcct cgacgtctcc gagggcgact acgtggtggg
      421 cgtgaacggc cagcggctca cggcctccat ggacccgtac cgcctgctcg acggcacgtc
      481 gggcctgcag accgtgctcc acgtgaatgg ccgcccgtcc atggacggcg cccggaccgt
      541 gacggtggag ccgatcgggg gcgagacggg cctgcgccag agggcgtggg tggaggacaa
      601 tcgccggaag gtcgacgagc tctcgggcgg acgcgtcgcg tacgcctgga tcccgaacac
      661 gggcgggggc ggcgtggtgt cgttcgaccg ttacttcttc gcgcagcagg accggcaggc
      721 ggccgtcatc gacgagcgct tcaacggcgg cggcctgctc gacgactaca tggtggacct
      781 catgacgcgg accctccgcg cggccatcac caacgaggtc ccgggcggga ggccgttccg
      841 gctgcccgcg ggcatcctgg ggccgaaggt gctgctcatc aacgagatgg ccgggtctgg
      901 cggggacttc ttcccgtggg tgttccgtca ccaggaggcc gggccgctca tcgggaagcg
      961 tacctggggc ggcctggtga agtcgtccgt gcactacgcg ctcatcgacg gcggcgcgct
     1021 cacggcgccc gacaacgccg tgttcgatcc cgtcgaggga cgattcatcg cggagaacga
     1081 gggcgtcccg ccggacatcg aggtcgagat cgatgcacgt tccgcggccg acggccggga
     1141 tccgcagctc gaacgggccg tcgaggaggc cctgaggctg ctcgacgagg ccgaactgcc
     1201 cgacgtccgg gctccgtcct tcccgcgacc gtcgaagcgt cccggaaact gaacggcccc
     1261 atggctgacc tgttcgaacg gctcgccgcc ctcggcgtcg ccttcgaccg cgtggaccac
     1321 cccccggtct tcacggtcga cgaggcgacc cgcctggtac ccccgctcca cggcgcgcgc
     1381 acgaagaacc tgttcgtgcg cgacaagtcg ggcaggcgcc actacctcgt gaccgtcggc
     1441 cacgggaagg cggtggacct gaaggccctc gcccgcacgg tcggggttcc gagcctttcc
     1501 ttcgggtcgc ccgaccgcct gaagcggcac ctgggcatcg aaccggggtc ggtgagcctg
     1561 ctcgccgtcg tcaacgacac ggccggggcc gtcgaggtga tcgtggaccg cgagctctgg
     1621 gcgagcgagc gcatccggtg ccatccgctc gtgaacacgg tgacgctctc gatcctgcgc
     1681 gccgacctgg aacggttcct cgacgacacg ggacaccggc cccgggtcat ggacgtgccc
     1741 gggcgggagg acggatgagt gcgcacatcc gccataccgc gtccttccgc gtccaccgac
     1801 ccgtcgacgt cgtcttcccg ctcttcagcc ccgaggggga acggctctgg gtacccggct
     1861 ggaactacgt ggacgtgatg ggcacgtccg agttgtcgga ggactacgtc ttcctcaccg
     1921 agtcccacga ccacgcctcg ggacaggccg tctggatcgt caagcgcttc gagcccgggc
     1981 ggcacctcgt cgcgttctac aaggtcgagc cgggagacaa ggtgggcgtg gtcacggtgc
     2041 ggtgccgcga ggaggcgcct ggcgtgacgg aggtcgaggt gacgtacgcc taccgggcgc
     2101 tgtcggaccg tggcctcgcg ttcgtcgagc gcttcaccgc agaggcgtac gaggcctaca
     2161 tcggggaatg gaaggagctg ctcgaccggt atttcgggaa ggggtgaccc cggtcggcgc
     2221 tgcccgtgtg ccggctcgcg tgcgccgcgc gtcagtcgtg ccgccgcaca tcgacggtca
     2281 ttccgcgatt gcgcccgcgg ccgaacgccg acaggtggta cacgaacttc agcatcacgt
     2341 accggcccag ggactcgatg cgctcctccc ggatccgggt cgcggagttc gagaagtcga
     2401 ccccctcgtt ctggtccagg agatcgaggc ccgtgagccg gatctcgacg cggtcgtcga
     2461 ggaacgtctt cgcgacggtc gcctccacga gcgccacgtc acggttctcc ccgaagacgt
     2521 cctgggcgta ggtccggtag tcgagcgacg ccgaaagggt ccacgacggg tgcgggtacc
     2581 aggtggcatc cgcgaagtac gtccggttca cgtaatcctg gttcagggcc cggttcagcg
     2641 agtagccgat ccggttgaac gtgtaacggg cgccgccccg cacgtcgaac aggtccttgg
     2701 tccggttctc gaggcgcgcc tcgacggtcg agcggaggat gtccgtcgag ttctggacct
     2761 ggttcacgaa ctcggagcct cgcgagaaga gggtcgaatt cgacaggctg atcttcgcgc
     2821 cgatgggccg gatgggtgtt ccgaagtcga cccggccgtt caccgtccag tcgtcgccgg
     2881 agttgacgga cgtcacggtc tgccggaact ggtcgtccac cgtgcgcgag cggacgatct
     2941 tgttgcgcgt gtgcgtcacc gccaccatcg agaagagatt cgtgaaggtg aactggtcga
     3001 acagcatgaa atggaggttc agcgtgtggc ggtactccgg cgtgagatcg ggattgccca
     3061 cgaagacgtt cagcgggtcc gagttgtcga cgaccggcga cagctccgtc atggacggct
     3121 cgcgcgtcga ggtgcggtac cgtgcgtcca ggttcatgcc ctgccggaac tgccagccga
     3181 tgctcgccga cgggaggagg tgcgtgtagc cgctcccgac gtcttcgtcc cggtccagga
     3241 tcctgccgtc gagctgcgag cgctggacct ccaggcccgc cccgagcgac agccgttcgt
     3301 cgttgtggcg gaagcgggaa ccgccccgcc agaacgtgtg cgtccggtcg aagcccgagc
     3361 tgagcgcgtc gttgaacacg ggctcgcccg acaccaggtc gtagatccgc ttgtcctggt
     3421 cctcgtccac gcggttgtgc tcggcgctga actcgagctg gcgccccgct ccgagcggct
     3481 cggtcagcga caggcgctgc gtctggctca gcgtgttgcc caggcgcgac tgctgctgca
     3541 cgatctcgtc gtacgtgacc aggtcgccct cctcgtacag gccggtcacc gactcgaggt
     3601 cggacgacac gtccgagtcg ttcaggttca cgcgggcctc ggcgaccagg ctgcgccccc
     3661 gttccgaaag ccgcctgcgc cacgtgagcg acgcgtctcc cccgaggtcg tcgccgtccg
     3721 acgcgtacgt ggccacgttc gtgttctgcg tgagccccgt cgccgtccgg gtcgtgcgcc
     3781 ctccctggtt caggagcgtc gagccggacg cctgcaggtt gccgcgaaac cgcaggtcgt
     3841 gcccgtcctg cagcttgtgg tccacgttca ggttcacccg gtgcgtgacg ttgtccgacg
     3901 tctggccgct cgactcgtta acgagcgacg acacggacga gcccagcagt tcttcctggt
     3961 tcaccgtccg gtcctgccgg ttgtcgatcg agctcatgaa ccacgaggag cgcaccgagg
     4021 tcttcgcgcc gaaatcgtgg ctggcgttca cgccgaaggc catcgtctcc gagaagccgt
     4081 cgctgatgtc cgtgcccagc tggatgccgc ccggccgcat cccgtccgac atgagcgtct
     4141 gcatgccgcc catgaagcgg atgtagtcct ggaacgagaa gccctgccgg ttgatgttgt
     4201 tcacgttggc gagcagcgag agctgcgtcc gcggcgtgaa ccggtttacc gaggcctgcc
     4261 cgtcgtagcg ctccgggtcg ccgtatccgc cggccacgtt cccgaagtac ccctgctgcg
     4321 cgtcgtcggt caggtccagg ttcagggtct tctcctcgtc gccgtcccgg atgcccgtga
     4381 actcggccat gtccgagagc ttgtcgtaca cctgcacctt gtccacggcc tccgcgggca
     4441 ggttgcgggt cgcgaccgtc gggtcgctgc cgaaaaactc cttgccctcg accagcacct
     4501 tcttcacgtc ctcgccctgc gccttgatcg agccgtcgtc cgcgacctcg atgccgggaa
     4561 gccgcttgag caggtcctcg accgtcgcgt tcggccgcac ggcgaaggcg cgcgcgttgt
     4621 actcgagcgt gtccttcttg acgacgaacg gcacgtggtc cgacgagatg acgagctcgt
     4681 cgagctccga gacgagctcg gtgagcacga gcgtcccgac gtccaggtcc tcgcccgaga
     4741 gcgtgacctc gcggctccag gtcgagtacc ccacgaaggt gacctgcagg acgtactctc
     4801 cggccggcac gcgcctcagc gtgaacgaac cgtcccggtc cgcgatcgcg aacttcacga
     4861 tcacggagtc gggcttcgag agcgcgacga cggtcgcgcc ccgcaggccc actccggcgg
     4921 tgtcgagcac gacgccgcgg acgtcacggc tgtcctgggc ggcgacgtcg gttgcgagga
     4981 ggagcgcggc ggcgaggagc acgccacgaa gccgtaaaag ggtcggtcgc atggggatcg
     5041 gcggggctga acggggcagc gtacgggagg atcgggcctc agttgccgat ccggacgtga
     5101 aacgtggccc cttcgcggcc acgctgtgcg cccatttcct tcatcttctc gtcaacgatc
     5161 gcgcggaatt cgtcccgggt gacctcgcgg ccgccctccg ggggcgtgag cacgccctcg
     5221 tcgagcgcgc cgagcgagac ctccttcgcc gtgatggaca tgcgcccgtc atccacgttc
     5281 acggcgagga tcaggcccgg caggccgccg tacggacccg gcccgcccgg aacggggatt
     5341 tcgggggtga accacgcctc gaccgtggtg ctgtcccggg tcgcaacggc cttcatgcag
     5401 gcgtagccca ggaattctcc ctgctcgccg gtgagccgcc acgcgtacgc ctccggctcg
     5461 cccgtcacga ggaacgtgcg cccgagaaag tcccgcttct cgatcacccg cccgccctcg
     5521 agatcggtgt agacctggtt gtccacgtcg cccgacgtca tcatccggat catcatgccc
     5581 tcgccgccga cccgcacgcc gctctcctcg ccgtccttct tcggagcgtt cttcatgagc
     5641 gtaccggcct cggagaagta aagcagcttc gtcgaggtcg acgagttcgg gatctggtcg
     5701 cgcatctgcg ccatctcggg gggcagctcg atctcgatct tgacgctctc gtcgtaggtc
     5761 acgacgccct cctgggcgcg ggccgggagc gtgaggaggg cgatggcgac gaggagtagc
     5821 ggacggcgca tggctgcggg gtggcgggtt gagacgaaga caacgccggg acggcgaatt
     5881 ggatcaaccg gaaaacgcgg gtgccgggcg gaatattgcg cggcacgggc ccacggcttg
     5941 cccggccgag tcgcgggggc ttttcttgcg tcatccgcca cccccgaccc ggcgacccat
     6001 ggcccggccg ttcgctaccc tcctcctgct cctctcggtc tcccccgccg ccgggcagtc
     6061 cgccggcgtg gacggcctcg cttggcttca gggctgctgg atccaggtgg acggcgagcg
     6121 cacgatcgag gagcactgga tgaccccgag gggcgacgcc atggtgggca tgagccggac
     6181 gagccggagc ggccgcctgc gcgcgtacga gctcgtcgtc atccactcgg actcgtccgg
     6241 tctcacgtat accgcccacc cgtccggaca acccggcgcc gcattccgct ccacgtccgt
     6301 cggcgacggg acggtcgcgt tcgagaatcc ggaccacgat tttccgcgcc ggatcgtgta
     6361 cgagcgcgcg ggggcggact ccctcctggc ctggatcgag gggccacccg gcggggagcc
     6421 ccgtcgaacc accttccgtt acggccggac accttgtccc gggcccggct gacatgaacc
     6481 tcgcgctccg accctccccg atcctggccc tcgcgctcgt cgcgacggcc tgcggcgctc
     6541 cgtccccgca ggaattcgac gagcagcgcc tcgcctcggt cgaggccgac gtccgcgcgt
     6601 tcgtctcgtc catcccgatc cacctcgccg agcgcggacc aacggcgtgg ctcgatcact
     6661 tcgaccgcgg tccggacttc ttcatggcct cggacggcgt ggaggtcttt cccgaccatg
     6721 ccacggccga cgagctcgtg cgggggtttg cgccgtcgat cgccgccatg gagatcacgt
     6781 gggactccgt ccgcgtgcag gtgctcacgc ccgactgggc cgtcttctcg accgcgtacc
     6841 gcgagaccct cacggatacg tccggggtgg acatcacgtt cgggggctac gtgacggcga
     6901 ccgtcgtccg gcgcgcggcc gggtggcgca tccagaacct gcactggtcc agcccggcgc
     6961 ccggcggctc gtgacgggcg gaccgacacc atgagagaga tgtcattgaa gacgactgcg
     7021 ttcctcgctc tcctcgtctc ggcggccgcg tgcgagcccg aggctccgga tccgatgcgg
     7081 gcggcgaccg catggggcag cccggcgtgg gaggtgcggc tcggggactc gaccgcgcac
     7141 tggataggcc tgcatgccct ggaccggaac gtggtctggg cggcggggcg gggcgggcgc
     7201 gtggcccgga ccacggacgg gggcgccacc tggacggtcg tcacggtgcc gggcgcggac
     7261 agcctgcagt tccgggacgc gcacgcgttc tcggccgacg aggccttcgt cctctcgatc
     7321 ggcaacggcc ctgattcgcg catctacgcg acgcgggacg ggggcgcgac gtggacggac
     7381 gtgtcggaca acgtgccgga cctcgggcat ggcatggtcg tgagccgtct cgagccgtcg
     7441 caccacgtgg ccgggcgggt ctatctgtcg ttcgacggcc attacagcga cgattatgcg
     7501 ccgtacgtcc acgtcagcga ggattacggc gcgagctggc gacggatcac cggcggcctt
     7561 ccggactggt cggtgaacgt cgtgaaggag catccccgca cgccggagct tctcttcgcc
     7621 gggaacgagg tgggcgtgta cgcgtcgttc gaccggggcg gtgcctggca ccgcctgccc
     7681 ggcc
//
LOCUS       DBNHQZ010000443         1953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2162421, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000443 DBNHQZ010000000
VERSION     DBNHQZ010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2162421"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /gene="ispG"
                     /locus_tag="AC16YU_11965"
                     /gene_synonym="gcpE"
     CDS             complement(<1..976)
                     /gene="ispG"
                     /locus_tag="AC16YU_11965"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR6065987.1"
                     /translation="MMDRPRRISNSVQVGPVTIGGGAPISVQSMTTSKTHDVAATLAE
                     VRELAEAGADIVRVAVPRPEDAAVLKEIVDGSPVPIVADIHFNHKYALQAIEAGVAKV
                     RINPGNIGKEEWEREVLHAAKAAGIPIRIGVNSGSLERDILDKHGYPKPEALYESAMR
                     HVEICSRAGFDDLVISVKHSDVYFMIQSYRLLAERTPYPLHLGVTESGSLRSGTIKSS
                     IGIGALLADGIGDTIRVSLATASVHEVEAGHQILKSLRIGRPGVNIIACPTCGRLAGD
                     MFPIVEAVEAAVVARGFDKDLSVAIMGCAVNGPGEAAGADLGVSLGRGR"
     gene            complement(1140..>1953)
                     /locus_tag="AC16YU_11970"
     CDS             complement(1140..>1953)
                     /locus_tag="AC16YU_11970"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6065988.1"
                     /translation="DLPRILFESWQLLAYAFLHLAPGMGGLLHVLFNLLWLVWIGRDL
                     EELQGPARLFGLYVIGAIGGALLTVALHAAFPAASLFSGIVHGASGAVLAVMAGVALL
                     YPDKQILLFLLGSIRLIHLVLGFLMLDILFLAAGGTSVSAHLGGALTGFVFARLQQRG
                     VDVVAWAAPFFRSRPRGARSRYRAESPSVLERLEGWLAGRRASGPMTRTQEPRAERKA
                     PSAGSSGEVAEGDRADEPAESDVDRILDKISESGYESLTAAEKRILYEASRR"
BASE COUNT          361 a          700 c          603 g          289 t
ORIGIN      
        1 cacgtccgcg gccgagcgag actccgaggt ccgcccctgc cgcctcgccc ggtccgttca
       61 ccgcgcaacc catgatcgcg acgctcagat ccttgtcgaa gccccgcgcc accaccgccg
      121 cctcgacggc ctcgacgatc ggaaacatgt cgcccgccag tcgcccgcaa gtgggacagg
      181 cgatgatgtt cacccccggc cgcccgatgc gaagggactt caggatctgg tgtccggcct
      241 ccacttcgtg cacggatgcc gtcgcgaggg acacgcggat ggtgtcgccg atcccgtcag
      301 cgagcaaggc cccgatcccg atggagctct tgatcgtgcc ggaacgcagc gaacccgatt
      361 cggtcacgcc caggtgcagc ggatatggcg ttctctcggc caacagccga tacgactgga
      421 tcatgaagta gacatccgag tgcttcaccg agatgaccag gtcgtcgaag ccggcgcgag
      481 agcagatctc gacgtgacgc atcgcgctct cgtacagcgc ttccggcttc ggataaccgt
      541 gcttgtccag gatgtcccgc tcgagcgagc ctgagttcac accgatccgg atcggaatac
      601 cggctgcctt ggccgcgtgc agcacttcgc gctcccactc ctcctttccg atgttgcccg
      661 ggttgatgcg gaccttggcc acgccggcct cgatggcctg cagggcgtac ttgtgattga
      721 agtggatgtc cgcgacgatg ggcacgggcg agccgtccac gatctccttc aaaacggccg
      781 catcctccgg gcgcggcacg gccactcgaa cgatgtccgc gccggcctcc gccaactccc
      841 ggacctccgc aagcgtggcg gcgacgtcgt gggttttcga cgtcgtcatg gactgcacgg
      901 aaatgggggc tcccccaccg atcgtgaccg gtcctacctg tacggagttc gagatgcgac
      961 ggggccgatc catcatgcct ggattgatct tgaggccgat acaaccatcg agccacaatc
     1021 gggttcggcg gaaacggctc ctccgtgctg gctgtctgcc tgaccagagc gaccgtcgaa
     1081 cccgaacagg ccgcgggggg ctcaaacccc gatccgcaaa aatccacgac gcagaccctc
     1141 taccgtctcg acgcctcgta cagaatccgc ttctccgccg cggtaagcga ttcgtaccca
     1201 gactcgctga tcttgtccag gatgcggtcg acgtccgatt cggccggctc gtcggcacga
     1261 tcgccctctg cgacctcccc ggatgaaccg gccgacggag ccttccgctc cgcccgcggc
     1321 tcctgcgtcc gggtcatcgg ccccgaagcc cggcggccgg caagccaccc ctccaggcgc
     1381 tccagcacgg acggcgattc cgccctgtac cgcgaacggg caccgcgagg gcgagagcgg
     1441 aagaacgggg ccgcccaggc cactacgtcc acgccccgtt gctgcagcct ggcgaacacg
     1501 aatcccgtca gcgcgccgcc caggtgcgca gacaccgacg tccctcctgc cgccaggaag
     1561 agaatgtcca gcatcaggaa cccaaggacc aggtggatca gtcggatcga gcccagcagg
     1621 aagagcagaa tctgcttgtc cggatagagc agggccacgc cggccatgac ggcgagcacg
     1681 gcgccggacg cgccgtgcac gatacccgaa aaaaggctcg cggccggaaa cgccgcgtgc
     1741 agggccaccg taagcaaggc cccgccgatc gccccgatca cgtagagccc gaacagacgg
     1801 gcgggcccct gaagctcctc caggtcccga ccgatccaca cgagccacag caggttgaac
     1861 agcacgtgca ggaggccgcc cattcccggg gccaggtgca ggaaggcgta ggccagcagc
     1921 tgccaggact cgaacaggat ccgtgggagg tcc
//
LOCUS       DBNHQZ010000444         2661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2163039, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000444 DBNHQZ010000000
VERSION     DBNHQZ010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2163039"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="AC16YU_11975"
     CDS             complement(<1..805)
                     /locus_tag="AC16YU_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6065989.1"
                     /translation="MSRTVRSAIRLASVAVFAAVLGCSAGSPEPASAQQGARLPHPPA
                     EGEKLDGMTIEEYDPVSTLRVPGQPVTTARYPYVDVHSHWFRAATLSNGAVDTLIAQM
                     DSLNLAVIVNLSGGSGERLAANIRGLEGRHPERFVTFANVSFDSIGAPGWTERAVARL
                     AADLDAGARGLKIYKNLGMDVRDVDGNRVPVDDPRLDPIWALCGERGVPVLIHTADPA
                     MFWEPRDRFNERWLELKLNPGRYRPPDRYPPFEDLMAEQYRMFAKHPGTT"
     gene            951..>2661
                     /gene="uvrB"
                     /locus_tag="AC16YU_11980"
     CDS             951..>2661
                     /gene="uvrB"
                     /locus_tag="AC16YU_11980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR6065990.1"
                     /translation="MDTLREGLPDGFRLEAPFVPTGDQPRAIEELVAGLERGDVHQTL
                     LGATGTGKTFTIANVIERIGRPTLVISHNKTLAAQLYAELKQFFPSNAVEFFISYYDY
                     YQPEAYIVHSDTYIEKDMSINDRIDRLRLRATSSLVSGRPDVIVVASVSCIYGLGSPD
                     EFRAEIVQLRRGAVIERNDLLRRLISIYYTRNDIEFQPGTFRVRGDVVEVFPAYLEDR
                     AYRIAFWGDEVEKISVFEPLTGHAIAEEPYLTVYPAKLFVTPRDQVERAIGSIEDELR
                     WRLAVLRDSGKVLEAHRLEQRTLFDIEMLREVGYCSGIENYSRHLSGRAPGDRPHCLL
                     DYFPDDFLMVVDESHVTLPQVRAMYNGDRARKLTLVEYGFRLPSALDNRPMTFEEFEA
                     RQHRVIYASATPGDYELELGGGVFVEQIIRPTGIPDPAIEIRPTTNQIDDLLHEVRTV
                     SEGGERTLVTTLTKRMAEDLADYLQSFGLRVRYLHSDIDALERVEILRGLRLGDFDVL
                     IGVNLLREGLDLPEVALVAILDADKEGFLRSDRSLIQTAGRAARNVNGRIVMYADTVT
                     GSMA"
BASE COUNT          419 a          913 c          916 g          413 t
ORIGIN      
        1 aggtcgttcc gggatgcttc gcgaacatcc ggtattgctc ggccatgagg tcctcgaagg
       61 gcgggtagcg gtccggggga cggtaccgcc ccgggttcag cttcagctcc agccatcgct
      121 cgttgaagcg gtcgcggggc tcccagaaca tcgcggggtc ggccgtgtgg atgagtacgg
      181 ggacgcccct ctctccgcac agcgcccaga tcgggtccag ccgcggatcg tcgacgggga
      241 cgcggtttcc gtccacgtcg cggacgtcca tgcccaggtt cttgtagatc ttcagccccc
      301 tcgcaccggc gtccaggtcc gccgcgaggc gcgccacggc ccgctccgtc cagccgggag
      361 cgccgatcga atcgaacgag acgttggcga acgtcacgaa ccgctcgggg tgacggccct
      421 cgagtccccg gatgttggcg gcgagccgct ccccgctgcc gcccgacagg ttcacgatga
      481 cggccaggtt cagcgagtcc atttgggcga tgagcgtgtc caccgctccg ttcgacaagg
      541 tggccgcccg gaaccagtgg ctgtgcacgt ccacgtaggg atatctcgcg gtcgtgaccg
      601 gctgccccgg tacgcgcagc gtcgaaacgg gatcgtactc ctcgatggtc atgccgtcga
      661 gcttctcgcc ctcggccgga ggatggggca gtcgcgcacc ctgctgcgcg gacgcgggct
      721 ccggggagcc ggcggagcat ccgaggacgg cggcgaaaac ggcgacggag gccaggcgga
      781 tcgcggagcg gacggttctg gacataggcg gaaggagtgg aggtggcgcc ccttggatgc
      841 ggcgcggccc gtcgtgtttc cccggacccg atgacgcgcc gcggcgttgg catcgcccga
      901 cgcacagcca gcacggctcc ggccccgcga tgtccgacga agtccgaagc atggataccc
      961 tccgtgaggg tcttcccgac ggcttccgcc tggaggcgcc gttcgtgccg accggcgacc
     1021 agccccgcgc gatcgaggag ctggtggcgg gcctggagcg gggagacgtc caccagacgc
     1081 tgctcggcgc gacgggcacg ggcaagacct tcacgatcgc gaacgtcatc gagcgcatcg
     1141 gccgcccgac gctcgtcatc agccacaaca agacgctggc ggcgcagctg tacgcggagc
     1201 tcaagcagtt cttcccgtcg aacgcggtcg agttcttcat ctcgtactac gactactacc
     1261 agccggaggc gtacatcgtc cactcggaca cgtacatcga gaaggacatg tcgatcaacg
     1321 accggatcga ccgccttcgc ctgcgggcca cgtcgtcgct cgtgtccggc cgtccggacg
     1381 tgatcgtggt ggccagcgtc tcctgcatct acggcctggg ctcgccggac gaattccgcg
     1441 ccgagatcgt ccagcttagg cgcggggccg tcatcgagcg gaacgacctg ctccggcggc
     1501 tcatatcgat ctactacacg cgcaacgaca tcgaattcca gccggggacg ttccgcgtac
     1561 ggggggacgt ggtcgaggtc ttcccggcgt acctcgagga ccgggcctac cgcatcgcct
     1621 tctggggcga cgaggtggag aagatctcgg tgttcgagcc cctgacgggc cacgccatcg
     1681 ccgaggagcc gtacctcacc gtctacccgg cgaagctctt cgtgacgccg cgcgaccagg
     1741 tggagcgggc catcgggtcg atcgaggacg agctccggtg gcgcctcgcg gtcctgcgcg
     1801 acagcggtaa ggtgctcgag gcccaccgcc tggagcagcg gacgctcttc gacatcgaga
     1861 tgctgcgcga ggtcggctac tgctccggga tcgagaacta ctcgcgccac ctctccggcc
     1921 gcgctccggg cgacaggccg cactgcctgc tcgactactt ccccgacgac ttcctgatgg
     1981 tcgtcgacga gagccacgtg acgctcccgc aggtccgggc gatgtacaac ggggaccgcg
     2041 cgcgcaagct cacgctcgtg gagtacggct tccgcctgcc gtcggcgctc gacaaccggc
     2101 cgatgacgtt cgaggagttc gaggccaggc agcaccgggt gatctacgcg tcggccacgc
     2161 cgggcgacta cgagctcgag ctcggagggg gcgtcttcgt ggagcagatc atccggccga
     2221 cgggcatccc ggacccggcc atcgagatcc gcccgacgac caaccagatc gacgacctgc
     2281 tgcacgaggt ccgcaccgtc tcggagggcg gcgagcggac gctcgtcacg acgctcacga
     2341 agcggatggc cgaagacctg gccgactatc tgcagtcctt cggccttcgc gtgcgctacc
     2401 tgcactcgga catcgatgcg ctggagcgcg tcgagatcct gcgcggcctg cgcctgggcg
     2461 acttcgacgt gctgatcggc gtgaacctcc ttcgcgaggg gctggacctg cccgaggtgg
     2521 cgctcgtggc catcctggac gcggacaagg agggattcct gcgctcggac cgctctctca
     2581 tccagacggc gggccgggcc gcgcgcaacg tgaacggccg catcgtgatg tacgcggaca
     2641 cggtcaccgg gtccatggcg c
//
LOCUS       DBNHQZ010000445        15456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3519869, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000445 DBNHQZ010000000
VERSION     DBNHQZ010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3519869"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16YU_11985"
     CDS             complement(<1..180)
                     /locus_tag="AC16YU_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065991.1"
                     /translation="MALFVPIMLVLLASARPAAAQDADTSGVRRVGLEARLCETRGGG
                     RQECFDVRLRDGSSTV"
     gene            complement(189..752)
                     /gene="lptC"
                     /locus_tag="AC16YU_11990"
     CDS             complement(189..752)
                     /gene="lptC"
                     /locus_tag="AC16YU_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR6065992.1"
                     /translation="MRHQAALLFACLTLAACERRAVPVSMETARGEDAPIQETWDAHL
                     VLSEAGIPRLDIRSAYQASFERGDSAFVLMTGTEAPGADRVTAVVYGESGDTTAVVRA
                     DRLYWYEEDERLEARGNVIVDAANGRRLESEHLEWDDAARTVTTPGFARIHSDDGVFE
                     GYGLEADEALEDARLNNVTGMLEVVDE"
     gene            complement(754..1446)
                     /locus_tag="AC16YU_11995"
     CDS             complement(754..1446)
                     /locus_tag="AC16YU_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6065993.1"
                     /translation="MIHVEIESGVEATLGVVRAEGVNSLSRPAVLEKAFAETLGQRAG
                     GLSPEEEALRSAVRDMLRHGRYRPTGRAKPASEYLLRAVSEGAFPRINAPVDVCNVVS
                     VGSLLPISVWDADRAGSRNYLVRHGMAGESYVFNATGQSIELEDLLLAARVGGPGERG
                     EPVVNPVKDSLLTRTDEASTHVAALIYAPARGFDARLSEAVERFARLVEACGTGVSVK
                     TAILRPPGSVEV"
     gene            1667..2005
                     /locus_tag="AC16YU_12000"
     CDS             1667..2005
                     /locus_tag="AC16YU_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6065994.1"
                     /translation="MTFSIEPWTGHTVVVHVGKALDFRNAANFKSACQDQVRDGVRNF
                     VLDFSETGILDSTGLGSIFSLYRQVSPLEGQVVFASVSRPVQVVVQLTRTYKVFRQFP
                     SLEAAREALQ"
     gene            2051..2503
                     /locus_tag="AC16YU_12005"
     CDS             2051..2503
                     /locus_tag="AC16YU_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6065995.1"
                     /translation="MVTTRHRFSDLDNVMDEVHSLFQHWEEDGLFEPLFDRDKIQLIR
                     LAVHEWMANLVQHADFSDRTPEIILDLHPNGERVRCVIEDNSEGFDLDVKLLDRMNGL
                     DKYPERGMGLLMLNAATEFLDYRRGTNGHHRLEFSVSADDNPWLSIPF"
     gene            2482..3648
                     /locus_tag="AC16YU_12010"
     CDS             2482..3648
                     /locus_tag="AC16YU_12010"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6065996.1"
                     /translation="MAKHSILVVEDEQTVRRLLEYRLAKEEYRVRTAANGEEALELIG
                     QELPDLVVSDIMMPVMDGFALQAAMQERRETRAIPFIFLTAKADEQSRLKGRRMGVDD
                     YITKPFDIEQLLSRVERLLDRTRQFSTQLDARIGQDFSKRLMPKRMPEVDGYRARFHN
                     APREQGGGDLFDWNSPEEGIYFFTIGDVMGKGLQAKVYAFSFLGYLRTMLKSLMRASR
                     SPADLLRQVNQMLLDDPPMEETFASLLLFRWEPAKHLVTFANAGHCRPVLAARSGAEI
                     LAHSDLILGLDRSAEYTDSSIEMPPGSALLAYTDGLLEQEMAAGHQLGEAGLKASVGR
                     VFGEPDPVQSLLDDVLALSSHREFKDDIMVFWLERLQIRERVTPRWFSPFADAR"
     gene            3763..4191
                     /gene="ndk"
                     /locus_tag="AC16YU_12015"
     CDS             3763..4191
                     /gene="ndk"
                     /locus_tag="AC16YU_12015"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR6065997.1"
                     /translation="MSVQRTLAILKPDCVEKDLVGEVIRRIQNAGFTLRALKMVRLTK
                     REAEGFYAVHRGRPFFEELTDFMSSGPCVPMVLEKEDAVADFRVLIGATDPAEAEPGT
                     IRRDLAESKGRNVVHGSDSVENGRLECGYFFPEHEIVANH"
     gene            4169..5449
                     /locus_tag="AC16YU_12020"
     CDS             4169..5449
                     /locus_tag="AC16YU_12020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR6065998.1"
                     /translation="MKSSPITDLRKGLLPMLLALAACAAPQDQPAADAPPALRTGAEI
                     LAESGFERLRGLRVGLIANHTTRVGNRHLSDLLAEAQDVELAALFGPEHGIRGDTDDG
                     VGIGDTLDAATGVPVYSLYGQRRSPPPGVLDSIDVLVFDIQDVGARFYTFISTMGHSM
                     QAAAAAGVPFVVLDRPNPLGGVRVDGFVLEPGHESFVGLYPIPVQHGMTVGELALMIR
                     GEGWLPGLDSLELDIVRMDGWARDRIWPDLGREWIPTSPNIPSFEAALVYPGTCFIEG
                     TTASEGRGTETPFLLIGAPWLDAARAASLLAEAGLPGVEFHPTRFTPVSIPGKDTHPK
                     HENVELAGIHIGVTDPRAVRPLAVGIHLLSAFASDPGAPEDFIRTAGLARLSGSGRLA
                     SFLAAGTDPDSVIATWSDEVAAFEIARRPYLLYE"
     gene            complement(5480..5695)
                     /locus_tag="AC16YU_12025"
     CDS             complement(5480..5695)
                     /locus_tag="AC16YU_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HIR6065999.1"
                     /translation="MVFERRNYILLLVGLALVVVGYVVMRVENEVDGFVSLYVAPLLI
                     LGGYLEIIYAILWRPAQGKPAADGDSG"
     gene            5889..6359
                     /gene="mreD"
                     /locus_tag="AC16YU_12030"
     CDS             5889..6359
                     /gene="mreD"
                     /locus_tag="AC16YU_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR6066000.1"
                     /translation="MPPFVRTVVLGLGVVAAQWLVFGRLRLWGSYPDAVLLYVAWLGL
                     RQGRRIGAFGGFSVGFLLDVIYDTWGLHTLIKTIVGFSLGMFPSAEREALLILPRQAF
                     LGGLVIALVQNGVLVILLALQTGTSDTFLVTGLWLGSALYTAVVGTLAALAAGR"
     gene            6449..6521
                     /locus_tag="AC16YU_12035"
     tRNA            6449..6521
                     /locus_tag="AC16YU_12035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6482..6484,aa:Ala,seq:ggc)
     gene            complement(6587..6787)
                     /locus_tag="AC16YU_12040"
     CDS             complement(6587..6787)
                     /locus_tag="AC16YU_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066001.1"
                     /translation="MSRFLERFPVLVFRIAMVAAAGVAVAALLVLVGVTWHDASLWVL
                     WVGVPVGLIVLVLWVIDLLRAR"
     gene            6890..7426
                     /locus_tag="AC16YU_12045"
     CDS             6890..7426
                     /locus_tag="AC16YU_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR6066002.1"
                     /translation="MADQRHLDLLKSGVAAFNAWREKNPDICPDLSGAELTGIDLRGA
                     DLSRAALADAFMTVAELDGCSFAEADLTGANFTGADATGADFHQANMRGADLSYAVLS
                     HADLRRADLRDAHFTEAILHGADLRHARMNGAVLKRAILIGANLAGAELDRADLEDAS
                     LDGATLPIGLGRSLRSDD"
     gene            complement(7456..7986)
                     /locus_tag="AC16YU_12050"
     CDS             complement(7456..7986)
                     /locus_tag="AC16YU_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HIR6066003.1"
                     /translation="MQSTQLSALSRPFDPGDIAWKPVATSPDRTTARVVPYVTNRAIM
                     DRLDLVCGPEGWRNEFRPGPNGGLLCGLSIRVVREDGPAEWITKWDGADNTDVQPVKG
                     GLSASMRRAAVQWGIGRYLYRVPPVYARIDEKGRLAERPRLPDAVAEVRADPAPKRPA
                     PVRALPRPMNGRTVAA"
     gene            8162..9472
                     /locus_tag="AC16YU_12055"
     CDS             8162..9472
                     /locus_tag="AC16YU_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6066004.1"
                     /translation="MPESTEGIIQAAVSAPDTAWILVSAALVLLMTPALAFFYGGLVR
                     SKNVLNTMMMSFVALGISAVVWVVAGYSLAFGEGSGPVGGLSMALLRGVGLEAKGTIP
                     HLLFMAYQGTFFIVTAALISGAVVERMRFGAYVPFIAAWGLLVYAPVCHWVWGGGWIG
                     SMGALDFAGGAVVHVNAAAAAVVAASTLGPRRDYGRLALLPHHVPFVLLGSGLLWFGW
                     FGFNAGSALAANGLAVLAFVNTLLAPAAALVVWMAIDHLRSGHVTAVGAATAIVVGLV
                     AVTPAAGFVSPLSALLVGALAAVPCYFAILGRARTRLDDSLDVFAAHGIGGIVGAVLT
                     GVFAEAAWNGTVNGLLFGNPGQVGIQVLSVVAVFAYSASATFVILKAIGRFVPLRAGA
                     TGERVGMDILHHGEEAYSDGEGAILIPNREIAPESVILPAPVPA"
     gene            9500..9853
                     /locus_tag="AC16YU_12060"
     CDS             9500..9853
                     /locus_tag="AC16YU_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6066005.1"
                     /translation="MKLIKAIIRPERLNDVLVALFHADVRGLTVSRVRGHGGERETQE
                     TYRGTTVRMELADKVQLDIGVSEPFVEPTVRAILGAARTGEVGDGKIFVLPVESVYRI
                     RTGEMDRSAVTPVAP"
     gene            9960..10580
                     /locus_tag="AC16YU_12065"
     CDS             9960..10580
                     /locus_tag="AC16YU_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6066006.1"
                     /translation="MDSVYLISLIVGGFFVLLSIFGGGDQEGDADADAEVDADSDFDH
                     DADVDHDFDHDVDAGHDLGAHADVGPGFVDLLSIRALFLFAAFFGLTGTVLGLVGSQE
                     PLTAVFSVLTGLVVGLGGNWLIKRFAYEQVSSAVSSLDLTGRTARVILPFVGPERGRI
                     LVEARGQRMQITARSLDTEGDDPFSPGDEVVVVRMDGRVAEVVKPE"
     gene            10638..11903
                     /locus_tag="AC16YU_12070"
     CDS             10638..11903
                     /locus_tag="AC16YU_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR6066007.1"
                     /translation="MELFVIPAILILGFLAVLGIVRANLKICQPNEVLIFSGRKRRLA
                     DGSPVGYRVIRGGRGFRIPIIERVDRLQLNTIPIDLTVTNAYSKGGIPLTVRAIANVK
                     ISSTEVELNNAVERLLGKPLEEIQTIAKETLEGNLRGVCASLTPEEVNEDRLKFAKVL
                     LDEADQDLGALGLQLDTLKIQSVEDDRGYLDAIGRQSTAHIIAAAEVVEAQKREQAKL
                     AEADADRAIVKAQTDVRIIKAKLDAQAEAEEAKINVAAQVARATAEQELAEQEIALAE
                     KRRYAEVVVAAEAEKKAKEEHAKGDAARITEDGKAEVDILRQKLELWKLAGNDAERLF
                     LIQMLPDILKEVIKTVDNLKIDKITVVDSGGGGGQGVPAVFHQIAGATPALLESLKAS
                     TGVDIAGLLSRASAPREDEVEEEKPAKGG"
     gene            complement(11929..12510)
                     /locus_tag="AC16YU_12075"
     CDS             complement(11929..12510)
                     /locus_tag="AC16YU_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066008.1"
                     /translation="MQYDPTNPTVAGLEAARRTSAFLRTLPTVGPGPEGLLVEALSSA
                     LRPGVALVRRPAVDTVAGTVTVLLAVVGPDRRIAVVDGSVSSAALALVYGTFDAVYAV
                     RADDLADRSPRVASAIAGSEVRLFTPDGRRALACCAESPAAAADRRAEDRQPVPIRHA
                     RRTVVRPLRLNRPGEWADAFERALEGARLSLSA"
     gene            13158..>15456
                     /locus_tag="AC16YU_12080"
     CDS             13158..>15456
                     /locus_tag="AC16YU_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6066009.1"
                     /translation="MKKLILIVAALFAVAGTQYALQLAPEAVITIEAWSPQQLHDAGL
                     TTHQSGGLRVVGLGSLVYLRGTEAGGEEVASYEWTATSPSGAVIPTDSSGTERTTFRP
                     DEIGTWTVGLTITTASGAAQTTATVVAGTYAGVGNLGGAIPDAGEGQCAACHPHNTST
                     WQGTGHSSKFTRAIDGIDHSFYAESCIECHTVGYDDSAVNGGFDDVASLLDWTFPAAI
                     QPGNWQDIVDNYAPLAQLANIQCENCHGPGSQHKGNKANIDISVEVGVCAQCHEEEPY
                     HLKPIMWRNSRHALGDFSRGTRTTCADCHSTYGFIARIDVQSNLDQQVGKGEGVTCAA
                     CHDPHEAGDGHPHQLRTIEDVTLVDGTEVTWGGHGKLCMNCHQARRTAYTQVIVQNST
                     RLDPHHSNQTDMLAANNVAQTFGRYIPSSTHRNALEDACVSCHMYETPAEGEPGHFLV
                     GDHTYAVRAEVDGQVVENVAVCERCHGVLESFSSVMARMDYDGDGVVEGVRDELHGVE
                     EELAMLLPPVGSPEVDQSSTTIREEYNLMQRWALFNLNFVHDDGSDGIHNFQYAMGLL
                     KVSIEAVKYGVISPGMITSVTDVPNDQGRMVRVVWERFGGDGVSTDPVARYIVLRNDG
                     PPAAGKAPLPNVEDLDLETTTDGTRMMLGGEGWTQVGSADAFQADEYSLVVPTLFDGQ
                     DTEFMVVAKTASSIYSVVSLPAVGMSADNLVPTAPMGLAAAATSWDVTLTWQALDLLH
                     NPDVDYYAVYRGETEGFVPGEPIATV"
BASE COUNT         2473 a         5390 c         5134 g         2459 t
ORIGIN      
        1 caccgtcgat gagccgtccc ggagccgcac gtcgaagcac tcctgccgcc ctcccccgcg
       61 agtttcgcac aggcgggcct ccaggcccac ccggcgtacc ccgctcgtgt cggcgtcctg
      121 cgccgcagcg ggacgcgcgg acgccaagag gaccagcatg atcggcacga aaagggccat
      181 gcgggcggtc attcgtccac gacctccagc atgcccgtga cgttgttcag ccgcgcgtcc
      241 tccagggcct cgtcggcctc caggccatag ccctcgaaga cgccgtcatc cgagtggatg
      301 cgggcgaagc cgggcgtcgt gacggtgcgc gccgcgtcgt cccactccag gtgctcggac
      361 tccaggcgcc gcccattcgc cgcgtcgacg atcacgttgc cacgggcctc gagccgctcg
      421 tcctcctcgt accagtacag gcggtccgcg cggaccacgg cggtcgtgtc gcccgactcg
      481 ccgtacacta cggccgtcac ccggtccgcc cccggcgcct ccgttcccgt catgagcacg
      541 aacgcgctgt cgccccgctc gaacgacgcc tggtacgccg accggatgtc gagccgggga
      601 atgcccgcct cggaaagcac gaggtgcgca tcccacgtct cctggatcgg ggcgtcctcg
      661 ccccgggccg tttccatgga cacgggaacg gccctgcgct cgcaggccgc gagcgtgagg
      721 cacgcgaata gcagggccgc ctggtgccgc atctcagacc tccaccgaac ccggcggacg
      781 gagtatcgcc gttttgacgc tcaccccggt accgcaggcc tcgaccaggc gcgcgaaccg
      841 ttccaccgcc tcggaaagcc gggcgtcgaa accccgggcg ggtgcgtaga tcagcgccgc
      901 cacgtgggta gaggcttcgt ccgtacgggt gaggaggctg tccttcaccg ggttcacgac
      961 gggctcgccc cgctcgcctg gcccgcccac ccgggccgcc agcagcaggt cctcgagctc
     1021 gatgctctga cccgtcgcgt tgaagacgta cgattcaccc gccatgccgt gccgcacgag
     1081 ataattccgg gagcctgccc ggtccgcgtc ccaaaccgag atcgggagga gcgagcccac
     1141 gctgaccacg ttgcacacgt cgacgggcgc gttgatccgg ggaaacgctc cctccgagac
     1201 ggccctgagc aggtattcgg acgccggctt cgcccggccc gtgggccggt accggccgtg
     1261 ccggagcatg tcgcggacgg cggagcggag cgcttcctcc tccggcgaca gccctcctgc
     1321 ccgctgcccg agcgtctcgg cgaaggcttt ttcgagaacc gcggggcgcg acagggaatt
     1381 cacgccctcc gcgcgcacca cgccgagtgt ggcctcgacg cccgattcga tctcgacgtg
     1441 aatcatggct gcgatcggaa cgacgggccc atcggaagtc ccggttttct atattcgatc
     1501 ccgtgctgcg cgggctgggt cccggttggg tggcagcatc gcaaaccgtc ccaaaatggc
     1561 gtttctggtg cgattctgac cctttagttc cccaacccgg cgtcgatact acgagccagg
     1621 gaccgcgtac acaaccagtg catcttcgac catgcaagct acttcgatga cgttttccat
     1681 tgagccctgg accgggcaca cggtggtcgt ccacgtcggc aaggcgctcg acttccgcaa
     1741 tgcggccaac ttcaagtcgg cgtgccagga ccaggtgcgc gacggggtcc gcaatttcgt
     1801 gctcgacttc tcggagaccg gaatcctgga ctcgacgggc ctgggatcca tcttctcact
     1861 gtaccggcag gtgtcgccgc tcgaaggcca ggtcgtcttc gcgtcggtat ccaggccggt
     1921 ccaggtcgtg gtgcagctca cgcgcaccta caaggtcttc cggcaatttc cgtccctgga
     1981 agcggcccgg gaggccctgc agtaagccga gtgccctgtc gtgcgcgcgc gccccgaaca
     2041 tgacgacagc atggtcacca cccgtcacag gttcagcgat ctggacaacg tcatggacga
     2101 ggtccattcc ctcttccagc actgggaaga ggatggactg ttcgagccgc tgttcgatcg
     2161 ggacaagatc cagctgatcc gcctggcggt gcacgagtgg atggcgaacc tggtccagca
     2221 cgccgacttc tcggaccgga cgcccgaaat catcctggac ctgcatccca acggggagcg
     2281 cgtccggtgc gtcatcgaag acaactccga gggcttcgac ctggacgtga agcttctcga
     2341 ccgcatgaac ggcctcgaca agtacccgga gagggggatg gggctgctga tgctcaacgc
     2401 cgcaacggag ttcctggact accggagggg aacgaacggc catcaccggc ttgaattctc
     2461 ggtatccgcc gacgacaacc catggctaag cattccattc tagtcgtcga ggacgagcaa
     2521 accgtacgcc gtctgctgga ataccgcctg gccaaggagg agtaccgggt gcgaacggcg
     2581 gcgaacggcg aggaggccct cgagctgatc gggcaggagc tgcccgacct ggtcgtctcc
     2641 gacatcatga tgccggtgat ggacggattc gcgctccagg ccgccatgca ggagcgccgc
     2701 gagacgcggg cgatcccctt cattttcctc accgcgaagg ccgacgagca gagccggctc
     2761 aagggtcgcc gcatgggcgt cgacgactac atcacgaagc ccttcgacat cgaacagctg
     2821 ctctcgcgcg tggaacgcct gctcgatcgc acccggcaat tctcgaccca gctcgacgcc
     2881 cggatcggac aggatttctc gaagcggctc atgcccaagc ggatgcccga ggtcgacggg
     2941 taccgggccc gcttccacaa cgcgccccgc gagcagggcg gcggagacct gttcgactgg
     3001 aactcaccgg aagagggcat ctacttcttc acgatcggcg acgtgatggg caagggcctc
     3061 caggccaagg tgtatgcgtt cagcttcctg ggctacctgc gcacgatgct gaagtccctc
     3121 atgcgcgcgt cgcgctcccc ggccgacctg ctgcgccagg tcaaccagat gctgctcgac
     3181 gatccgccga tggaagagac cttcgcctcc ctcctgctct tccgctggga gcccgcgaag
     3241 cacctggtca cgttcgcgaa cgccgggcac tgccggcccg tcctcgcggc caggagcggg
     3301 gcagagatcc tcgcccattc ggacctgatc ctggggctgg accgatccgc cgagtacacg
     3361 gactcctcga tcgagatgcc gccgggcagc gcgctgctcg cctacacgga cggtctcctg
     3421 gagcaggaaa tggcggcggg ccaccagctg ggcgaagcgg gcctgaaggc gtccgtcggc
     3481 cgcgtcttcg gggagccgga tccggttcag agcctgctcg acgacgtgct ggcgctttcg
     3541 agccaccggg agttcaagga cgacatcatg gtgttctggc tcgagcgtct gcagatccgg
     3601 gagcgggtca cgccccggtg gttctctccg ttcgcggacg cgcgctgaga cgaggtttca
     3661 ccgctaattc cccccggcac gggcacggtg ccgcgcggat ggcgtacgct tgcgccgacc
     3721 ggattccggt ccgcatcttc cgtacgaacc agccggcccg ccatgtccgt ccagcgcacg
     3781 ctcgccatcc tgaagcccga ctgtgtcgag aaggacctcg tcggtgaggt catccgccgg
     3841 atccagaacg caggcttcac cctgcgtgcc ctcaagatgg tgcgcctcac gaagcgcgag
     3901 gccgagggtt tctacgcggt ccaccgcggg cgtccgtttt tcgaggaact gacggacttc
     3961 atgtccagcg gcccgtgcgt gccgatggtg ctcgagaagg aggacgccgt cgccgatttc
     4021 cgcgtgctca tcggggccac cgacccggcc gaggccgagc cgggtaccat tcgccgcgac
     4081 ctggcggagt ccaagggccg gaacgtcgtc cacggatccg actccgtcga gaacggccgg
     4141 ctggagtgcg gctacttctt ccccgagcat gaaatcgtcg ccaatcactg acctccggaa
     4201 ggggctcctt ccgatgctcc tcgccctcgc cgcctgcgcc gcgccccagg accaacccgc
     4261 cgccgacgcg ccgccggcgc tgcggaccgg ggccgagatc cttgcggaat cgggattcga
     4321 gcgcctcagg ggactccggg taggcctcat cgcgaaccac acgacccggg tcggcaaccg
     4381 ccacctctcg gacctgctcg cggaggccca ggacgtcgag ctcgcggccc tcttcggtcc
     4441 cgagcacggg atccgcgggg acaccgacga cggggtcggc atcggcgaca cgctcgacgc
     4501 cgccaccggc gtaccggtct acagtctgta cggacagcgg cggtctccgc cgcccggcgt
     4561 gctggactcg atcgacgtcc tcgtcttcga catccaggac gtgggcgccc ggttctacac
     4621 cttcatatcc acgatggggc actccatgca ggccgccgcc gcggccggtg tcccgttcgt
     4681 ggtgctggat cggcccaatc ccctcggcgg cgtccgggtg gacggcttcg tcctggagcc
     4741 cggccacgag tcgttcgtgg gcctgtaccc gatcccggtg cagcacggga tgaccgtcgg
     4801 cgagctcgcg ctgatgatcc gtggagaggg gtggcttccc ggcctcgact cgctcgagct
     4861 ggacatcgtg cggatggacg ggtgggcgcg tgaccggatc tggcccgacc tcggacggga
     4921 gtggattccg acaagcccca acatcccgag cttcgaggcg gcgctcgtgt accccggcac
     4981 ctgcttcatc gagggtacga ccgccagcga ggggcgcggg accgagacgc cgttcctgct
     5041 catcggcgct ccctggctcg atgccgcgcg ggcggcatcg ctcctggcgg aggccggcct
     5101 gccgggggtc gaattccacc cgacccgctt cacgcccgtg agcattccgg gcaaggacac
     5161 ccacccgaag cacgagaacg tggagctcgc gggcatccat atcggtgtga cggatccgcg
     5221 cgccgttcgg ccgctcgccg tcggcatcca cctgctctcc gccttcgcgt ccgatcccgg
     5281 cgcccccgag gacttcatcc gcacggccgg cctggcgcgg ctgtcggggt cgggccggct
     5341 cgcctcgttc ctcgcggccg ggaccgatcc ggactcggtg atcgccacgt ggagcgacga
     5401 ggtggccgcc ttcgagatcg cgcggcgccc gtacctcctg tacgagtgaa ccgggctccg
     5461 gcgaaggttc cggtccggat cagccggaat cgccgtctgc ggccggcttg ccctgggcgg
     5521 gccgccacag gatggcgtag atgatctcca ggtagccccc gaggatcagg aggggcgcga
     5581 cgtagagcga gacgaatccg tccacctcgt tctcgacccg catgacgacg tagcccacca
     5641 cgacgagcgc gagacccacc aggagcagga tgtagttcct gcgctcgaag accatgggcg
     5701 ccacgtgacg agcgcctttc tgccggcggg aagcgggtcg gggcctggcc atgctcgggg
     5761 cgtcgcgctg cgcgggattc ggggctgcct gggaattgct ccgggtccaa tccggtcgcg
     5821 tcgtgcttgt tccgaatccg tcctcccgta gattccggcc ctcgagccac cgaaacgcag
     5881 tccgagccat gccccccttc gtccgaaccg tggtgctggg cctgggcgtc gtcgcggccc
     5941 agtggctcgt gttcggcagg ctccggctct ggggctcata tccggacgcc gtcctgctct
     6001 acgtcgcctg gctggggctc cgtcagggcc gtcgcatcgg cgcgttcggc ggcttctccg
     6061 tgggcttcct gctggacgtg atctacgaca cgtggggtct gcacacgctc atcaagacga
     6121 ttgtcggctt cagcctgggc atgttcccgt ccgcggagcg ggaggccctc ctgatcctcc
     6181 cgcgacaggc gttcctgggc ggcctggtca tcgccctcgt ccagaacggc gtcctcgtga
     6241 tcctgcttgc cctccagacc ggaacgagcg acaccttcct ggtcaccggc ctgtggctcg
     6301 gctcggcgtt gtacacggca gtcgtgggta cgctggccgc cctggcggcc ggccgctgag
     6361 cccggaaccg gcatcccgct cgggggtgga aggcggccgc gcgggttgat cggcgccgga
     6421 agccgaccgt attttgcgcg ggacctccgg ggctatagct cagctggtag agcgcttgaa
     6481 tggcattcaa gaggtcaggg gttcgactcc ccttagctcc accccaaggc ccggaccggg
     6541 atcggttcgg gcctttctgc tgcccgaggg caggccgaac cggactttac cgggcccgca
     6601 ggaggtcgat cacccagagt acgaggacga tcagtccgac gggaacaccg acccacagca
     6661 cccacaggga cgcgtcatgc caggtcacgc cgacgagaac gaggagcgcg gccacggcca
     6721 caccggccgc ggccaccatg gcgatacgga acacgaggac ggggaaccgt tcgagaaagc
     6781 ggctcacggg caacggaaac tgtcgtcgaa tgcgggaaag taaccgattc atccgtctgg
     6841 tccaccctcc cttccggaac ggggcggacc accgagagag agagcgagca tggccgacca
     6901 gcgacacctg gatctcctga agagcggagt ggccgcgttc aacgcgtggc gtgagaagaa
     6961 cccggacatc tgtcccgatc tctccggcgc cgagctcacc ggcatcgacc tgcgcggagc
     7021 cgacctcagc cgcgccgccc tcgccgacgc gttcatgacc gtcgccgagc tggacggatg
     7081 ctccttcgcc gaagccgacc tgaccggggc caacttcacg ggcgcggacg cgacgggagc
     7141 cgacttccac caggcgaaca tgcggggtgc cgacctctcc tacgccgtgc tcagccacgc
     7201 ggacctgcgc cgcgcggacc ttcgggacgc gcacttcacc gaggccatcc tccacggagc
     7261 cgacctccgg cacgcccgga tgaacggcgc cgtcctgaag cgggccatcc tgatcggcgc
     7321 caacctggcc ggggcagagc tcgaccgtgc agacctcgag gacgcgtcgc tcgacggcgc
     7381 gacgctcccc atcgggctcg gccggtcgct tcgctcggac gactgacccg gtgccggcca
     7441 cgcgccggct cgcggtcagg ccgccacggt ccgcccgttc atgggccgcg gcagggcccg
     7501 caccggcgcc ggccgcttcg gcgccgggtc cgcgcgcact tccgcgacgg catccggaag
     7561 acgggggcgc tcggccagcc ggcccttctc gtcgatgcgg gcgtacacgg gcggaacccg
     7621 gtacaggtat cggccgatcc cccactgcac cgccgcgcgc cgcatgctgg ccgacaggcc
     7681 ccccttcacg ggctgaacgt ccgtgttgtc cgcaccgtcc cacttcgtga tccactcggc
     7741 cgggccgtcc tcgcgcacca cgcggatgga aaggccgcac aggaggcctc cgttcgggcc
     7801 gggacggaat tcgttgcgcc atccctccgg accgcatacc aggtccagcc ggtccatgat
     7861 cgcccggttc gtgacgtagg gcacgacgcg cgccgtcgtc cggtcgggcg aagtcgcgac
     7921 gggcttccag gcgatgtcgc cgggatcgaa gggacgggac agggcggaaa gctgggtgct
     7981 ctgcatgggg gttgctcgct gttgttgaat gcaaggtcgg atgggagtgt gacagtgttt
     8041 tgtcatatcc tgttgagaat cgtacaatgg ccatcactgc cgatgtttga caagtgcatt
     8101 gaatctttcg cctcatttta ttagtcttgt tgtgcgtttc accttcgtta ctgtttctcc
     8161 catgcccgaa tccaccgagg ggatcatcca ggcggccgtt tccgccccgg atacggcctg
     8221 gatcctggtg tcggcggcgc tcgtgctgct catgacgccg gcgctcgcct tcttctacgg
     8281 tggcctggtc cgctcgaaga acgtgctcaa cacgatgatg atgagcttcg ttgcgctcgg
     8341 catctcggcc gtcgtgtggg tcgtcgcggg ctattcgctc gcattcggcg agggctccgg
     8401 cccggttggc ggcctgtcga tggccctgct gcgcggcgtc ggcctcgagg cgaaggggac
     8461 cattccgcac ctcctcttca tggcctacca ggggacgttc ttcatcgtca ccgcggcgct
     8521 gatctcgggc gccgtcgtag agcggatgcg cttcggcgcg tacgtgccct tcatcgcggc
     8581 ctgggggctc ctcgtgtacg cgcccgtctg ccactgggtg tggggcggcg gctggatcgg
     8641 gtcgatgggg gcgctcgact tcgcgggcgg cgccgtggtc catgtcaacg ccgccgcggc
     8701 cgccgtcgtc gccgcatcga ccctcggacc gcgccgtgac tatggccggc tcgccctgct
     8761 cccgcaccat gttccgttcg tgctcctggg ctccgggctg ctctggttcg ggtggttcgg
     8821 cttcaacgcg ggctctgccc tcgccgccaa cgggctcgcc gtcctggcgt tcgtcaacac
     8881 gctcctggcc cccgcggccg cgctcgtggt ctggatggcg atcgatcacc tccgatccgg
     8941 gcacgtgaca gccgtgggcg ccgcgacggc catcgtcgtg gggctcgtcg ccgtgacgcc
     9001 ggccgccggc ttcgtctcgc cgctgtccgc cctgctcgtg ggcgcgctcg ccgcggtccc
     9061 gtgctacttc gccatactgg gccgtgcccg aacccggctc gacgacagcc tggacgtgtt
     9121 cgccgcgcac gggatcgggg gcatcgtcgg cgcggtcctc accggcgtgt tcgccgaggc
     9181 cgcctggaac ggcaccgtga acggcctgct cttcggcaac ccgggccagg tcggcatcca
     9241 ggtgctctcg gtcgtggccg tcttcgcgta cagcgcgtcg gcgaccttcg tgatcctgaa
     9301 ggccatcggg cggttcgtcc cgctcagggc cggagcgacc ggggagcgcg tgggcatgga
     9361 catcctgcat cacggagagg aggcgtattc ggacggcgaa ggggcgatcc tcataccgaa
     9421 ccgggagatc gcgcccgagt ccgtcattct ccccgccccc gtcccggcct gagccgggca
     9481 caatcagccg gcacgaacca tgaagctcat caaggcgatc atccggcccg agcgcctgaa
     9541 cgacgtcctc gtcgcgctct tccatgccga cgtccggggc ctgaccgtca gccgggtccg
     9601 cgggcacggg ggcgagcgag agacccagga gacgtaccgc ggcaccaccg tccgcatgga
     9661 gctcgccgac aaggtccagc tggacatcgg cgtctcggag ccgttcgtcg aacccacggt
     9721 gcgggccatt ctcggcgccg cccggaccgg ggaggtcggg gacgggaaga tcttcgtact
     9781 tccggtcgag agcgtgtatc gcatccggac cggcgagatg gaccggtccg cggtgactcc
     9841 cgtcgcgccg tagcggatct cgcgggggtc tcccccacgc catcgcgagc ccggcgactg
     9901 ttgacgcgcc ccgcaaccgc gtctatcatg ccggcgtatc gtatggtcgg tgccccggca
     9961 tggattcggt ctacctgata agcctgatcg tcggcggctt cttcgtcctg ctttcgatct
    10021 tcggaggcgg cgaccaggag ggcgatgcgg atgcggacgc cgaggtcgat gcggattccg
    10081 acttcgacca cgacgcggac gtcgatcacg atttcgacca cgacgtggac gccggtcacg
    10141 acctgggcgc gcacgccgac gtgggccccg gcttcgtgga tctcctctcg atccgggcgc
    10201 ttttcctttt cgcggccttc ttcgggctca ccggaaccgt gctgggcctc gtcggctcac
    10261 aggagcccct gacggccgtg ttctcggtgc tcaccggcct cgtcgtcggc ctgggcggca
    10321 actggctgat caagcggttc gcctacgagc aggtgtccag cgccgtgtcc tcgctggatc
    10381 tcacgggacg cacggcccgc gtgatcctgc cgttcgtcgg gccggagcgc ggcaggatcc
    10441 tggtcgaggc ccggggccag cggatgcaga tcaccgcccg gagcctggac accgagggcg
    10501 acgatccgtt ctcgccggga gacgaggtgg tcgtggtccg gatggacggc cgcgtcgcgg
    10561 aagtggtcaa gccggaatga tcaacccagc ggaccacgcg tccgcccacg ggcgccccct
    10621 gggcgcggag tgacgacatg gaactgttcg tgataccggc catcctgatc ctgggctttc
    10681 tcgcggtcct ggggatcgtt cgtgccaacc tgaagatctg ccagcccaac gaagtcctga
    10741 tcttcagcgg ccgcaagcgc cggctcgccg acggaagccc cgtcggctac cgggtgatcc
    10801 ggggcgggcg cggattccgg ataccgatca tcgagcgggt ggaccgcctc cagctcaaca
    10861 ccatcccgat cgacctcacc gtcacgaacg cctactcgaa gggaggcata ccgctaacgg
    10921 tgcgcgcgat cgcgaacgtc aagatctcgt ccaccgaggt ggagctgaac aatgccgtcg
    10981 agcgcctgct cggcaagccg ctcgaggaga tccagaccat cgccaaggag acgctcgagg
    11041 gcaacctgcg cggcgtgtgc gcgtccctca ctcccgagga ggtcaacgag gaccggctca
    11101 agttcgcgaa ggtcctcctg gacgaggccg accaggacct cggcgcgctg ggcctgcagc
    11161 tggacacgct caagatccag agcgtggagg acgaccgcgg ctacctggat gccatcggcc
    11221 gccagtcgac ggcgcacatc atcgcggcgg ccgaggtagt cgaggcgcag aagcgcgagc
    11281 aggccaagct ggccgaggcc gacgccgacc gggcgatcgt gaaggcgcag accgacgtcc
    11341 gcatcatcaa ggcgaagctc gacgcgcagg ccgaggcgga ggaggcgaag atcaacgtcg
    11401 ccgcccaggt cgcgagggcc acggccgagc aggagctggc ggagcaggag atcgcgctcg
    11461 ccgagaagcg ccgttatgcg gaggtcgtcg tggcggcgga ggccgaaaag aaggccaagg
    11521 aggagcacgc caagggcgac gccgcgcgca tcaccgagga cggcaaggcc gaggtggaca
    11581 tcctgaggca gaagctggaa ctctggaagc tcgcgggaaa cgacgccgag cggctcttcc
    11641 tgatccagat gcttcccgac atcctgaagg aggtcatcaa gaccgtcgac aacctgaaga
    11701 tcgacaagat caccgtcgtc gactccggag gtggaggcgg ccagggggtc cccgcggtgt
    11761 tccaccagat cgccggtgcg acgcccgcgc tgctcgagag cctcaaggca tccacgggag
    11821 tcgacatcgc gggcctgctc agccgcgcat cggccccccg ggaagacgag gtagaggagg
    11881 agaagcccgc caagggcggc tgaccggcac gccgccttcc ggagacggtc aggcgctcag
    11941 ggagagccgg gcaccttcca aggcacgctc gaacgcgtcg gcccattcac ccggccggtt
    12001 cagtcgaagc ggacggacca ccgtgcgcct cgcgtgccgg atcggtacgg gctgcctgtc
    12061 ctccgccctg cggtcggcgg ccgcggcggg cgactccgca cagcaggcca gcgccctccg
    12121 cccgtccggc gtgaacaggc gcacctcgga accggcgatg gccgaggcga cccgtggcga
    12181 gcggtccgcc aggtcgtcgg ctcgcacggc atacaccgcg tcgaacgtcc cgtacaccag
    12241 cgccagggcc gcgctcgaaa cggacccgtc caccacggcg atccgccggt ccggtcccac
    12301 cacagccagg agcaccgtga ccgttcctgc gaccgtgtcc accgccggtc ggcgcaccag
    12361 ggccacccct ggccggagcg ccgaggacag cgcctccacc aggagccctt ccggaccggg
    12421 cccgaccgtg gggagtgtgc gaaggaacgc cgacgtgcga cgagcggcct cgaggcctgc
    12481 aacggtgggg ttggtcggat cgtactgcat ggagctctcc gtctggtccg gaagcaggat
    12541 acgtacgggg gtatgacagc gatttgtcat atcccttgtt gtgcctggtt ctcccttccg
    12601 cagtgccgtc gcgcgggccc tccgcgggcg tctcgcggac gactcctgtt gcccgggtga
    12661 aggagtcatg aatccgtagg ggcgccgcct ccgaccagga ccccgggcgg gtaccggatc
    12721 ccgtcggccc gtccgcggat cggccgtcgc ggacgcgcag aattgtcgcg cgcagggtca
    12781 ggaagcgagt ttcgggcgaa tcaggaatcc ccatcgcggg tgcacgcaaa cgggcggctt
    12841 tgttgtaggg cttctcccca ccattacggt aggtggtccc ctacgactat atcggatttg
    12901 gactacgccg gaggcgtcct ctcgccggta ttattccgca gtcgaaatcc tatggcgctg
    12961 caggcatgtc caccacacgg ttaccgggcg aatcgccctg ccgtcccgac cagggagtgg
    13021 ccgacgaacc gcgtggcgcc ttttcgatcc tgtaccacgg ggcgacccgc acccgggcat
    13081 tgggcccgcg atgcggccga cccggataat gaaccaaacg ggagcggtta gtccgctgcc
    13141 gggagcaaac caacaatatg aagaagttga tactgattgt ggccgcgctg ttcgcggtcg
    13201 ccggtaccca gtacgctctt cagctcgctc ctgaggccgt cattaccatc gaggcctgga
    13261 gcccgcagca gcttcacgat gcaggtctga ccacgcacca gtccggcgga ctgcgcgtcg
    13321 taggcttggg ttcactcgtg tatctgcgcg gtacggaagc tggaggcgag gaagtcgcct
    13381 cctacgagtg gacggcgacg tcgccgagcg gcgcagtgat tccgaccgat tcctccggga
    13441 cggagcgcac gacgttccgg cccgacgaaa tcggcacctg gaccgtcggc ctgaccatta
    13501 ccacggcatc cggtgctgca cagaccactg ccacggtcgt cgcaggcacg tacgccggcg
    13561 tgggcaacct gggcggtgca attcccgacg caggcgaggg acaatgcgcg gcgtgccacc
    13621 cgcacaatac ctccacctgg cagggcacgg ggcactcgtc gaagttcacg cgggcgatcg
    13681 acggcatcga ccactccttc tacgcggagt cctgcatcga atgtcacacc gtcggatacg
    13741 acgattcggc tgtcaacggt ggtttcgacg acgtggcctc gctccttgac tggacgttcc
    13801 ccgcagcgat tcaacccgga aactggcagg acatcgtaga caactacgct ccgctggccc
    13861 agctcgccaa catccagtgc gagaactgcc acggtcccgg ctcgcagcac aagggcaaca
    13921 aggccaacat cgacatctcg gttgaggtcg gcgtctgcgc ccagtgtcac gaggaggaac
    13981 cctaccacct caagccgatc atgtggcgca attcgaggca cgccctcgga gacttctccc
    14041 gcggaacgag aaccacgtgt gccgactgcc actcgaccta cggcttcatt gcccggatcg
    14101 acgttcagtc aaacctcgac cagcaggtcg gcaagggcga gggcgtcacc tgcgcagcgt
    14161 gccacgaccc gcacgaggcg ggtgacggcc acccgcacca gctccgcacg atcgaggacg
    14221 tcaccctcgt cgacggcact gaggtcacgt ggggcggcca cggcaagctc tgcatgaact
    14281 gccaccaggc ccgccgcacg gcctacaccc aggtcatcgt ccagaactcg acccgcctgg
    14341 atccgcacca ctcgaaccag acggacatgc tcgcggcgaa caatgtcgcg cagacgttcg
    14401 gacgctacat cccgagctcg acgcaccgca acgcccttga ggatgcgtgc gtctcgtgcc
    14461 acatgtacga gactccggcc gaaggcgaac ccggtcattt cctcgtgggc gaccacacct
    14521 atgccgttcg cgcagaggtg gatggccagg ttgtcgagaa cgtcgccgtc tgcgagcgat
    14581 gccacggcgt gcttgagtcc ttctcgagcg tgatggcgcg catggactat gacggcgacg
    14641 gcgtcgtcga gggagtccgc gacgagctgc acggcgtcga ggaggagctg gccatgctgc
    14701 tgcctcccgt cggatcaccc gaggtcgacc aatccagcac caccatccgc gaggagtaca
    14761 acctcatgca gcgctgggcg ctgttcaacc tgaacttcgt ccacgacgac ggctccgacg
    14821 gcatccacaa cttccagtat gccatgggcc tgctgaaggt gtccatcgag gccgtcaagt
    14881 acggggtgat ctctcccggc atgatcacct cggtcaccga cgtcccgaac gaccagggca
    14941 ggatggtccg cgtcgtgtgg gagcgcttcg gcggcgacgg tgtctcgacc gacccggtcg
    15001 ccaggtacat cgtgctgcgc aacgacgggc cgccggccgc cggaaaggca ccgctgccga
    15061 acgtcgagga cctggatctg gagacgacga ccgacggcac ccgcatgatg ctcggaggcg
    15121 aaggttggac gcaggtcggc tcggccgacg ccttccaggc cgacgagtac agccttgtcg
    15181 tcccgacgct gttcgacggg caggacacgg agttcatggt ggtggccaag accgcaagca
    15241 gcatctactc cgtggtgagt ctgccggcgg tcggcatgtc tgcggacaac ctcgtcccga
    15301 cggccccgat gggccttgcg gccgcggcca cgtcctggga cgtgaccctc acgtggcagg
    15361 cgctcgacct cctgcacaac cccgacgtcg actactacgc cgtgtaccgg ggcgagaccg
    15421 agggcttcgt gccgggcgag ccgatcgcga cggtga
//
LOCUS       DBNHQZ010000446         6591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2029914, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000446 DBNHQZ010000000
VERSION     DBNHQZ010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2029914"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="AC16YU_12085"
     CDS             <1..220
                     /locus_tag="AC16YU_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066010.1"
                     /translation="AQGIRGVAFGGGCVWMALPGRIVAFSPGGVLASDWAIELDGPVV
                     DLAVRDEGLVLLTARSLYVASAACAPEP"
     gene            complement(221..1798)
                     /locus_tag="AC16YU_12090"
     CDS             complement(221..1798)
                     /locus_tag="AC16YU_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6066011.1"
                     /translation="MISLIGGATDLTFAASETSPDMSISAENVRLILGLKLKNLRHAR
                     GLSLKDLAAGSGLSISYLSEIEKGKKYPKPDKLLALSTMLEVPFDELVSVSVDESLHP
                     LADTLRSPFFREFPFELFGLATQDLVSLFSSDPARAGALLRTFLEIGQMYDVRVEHFL
                     FAALRQYQLMHDNYFAEIEAVADGFRAEVGPEAVDGDAEGVLRAYLEQAFGYRVDEEM
                     LEERSDLAGFRSVFADGPEPVLYVHPRLLPSQKAFVYGREIGYRLLGLRERAVTSSWL
                     QVESFEQVLNNFRASYFAGAVLIARDDILDTLGHLFRRRSFDGDALLQAVHRLGTTPE
                     TFLYRVGQLLPHMAGFQEFFFLRVTLRPESDRVELTKVFNRSRIALPQGFWLDEHYCR
                     RWSGLRLLQQDGGSGEYGVHAQRSHFTREGVSFFVFSLSHPLTLSSGARTSITLGFLI
                     DDNFRQKVRFWDDPAVPDEEVNVTCERCPLSPEACSERVAEPVLLRSVESREAKEQAL
                     ARLLELGRKGAAPTVRA"
     gene            1956..2606
                     /locus_tag="AC16YU_12095"
     CDS             1956..2606
                     /locus_tag="AC16YU_12095"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR6066012.1"
                     /translation="MRIALFGPPGAGKGTQAALIGYRYGFRSISTGELIRTAMRDGTP
                     IGTEARRYVEDGTLVPDLLVSRLAEAAVEECDFDGFILDGYPRTVGQARSLDAFLDRH
                     DAALDALICLHVPDAELVDRLSARRVHRKTGTTYHLVYNPPPPGVPPDLLIRRPDDHP
                     DAIRRRLSEYRSVTEPVMAWYGDHLRLVRVDASGTVSEVALRLTTVLERLAVRALA"
     gene            2673..3932
                     /locus_tag="AC16YU_12100"
     CDS             2673..3932
                     /locus_tag="AC16YU_12100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6066013.1"
                     /translation="MTTYGERVLRQEVGPCTLLVLPTGAKDVVSVRLSIDTHPDFAAG
                     EELVQDVTAMLLQKGTRRRDRFEVASILEDRGARLSFSGSGVRVRASGRMLRGDVGVI
                     VRLAGEMLTEPAFDEDELSKTRLRVKGSVLRSASDTGHRAAVELARHALEPGHPGFMA
                     RPDEDLAALESLDGRAIRAFHASRFRGPVRCVAVGDVDAAALREAIDEAFSAWPSGPS
                     RGVAYPDSPATVPGAVRTPIPDRPNLDVVLGHSLPTRRDDQEFDALHAGVFALGGNFS
                     ARLMAHVRDELGLTYGIGSSLSGIGIHHGGLWQTSLTLSADRLSEGIDATRRELDRFV
                     SEGVAPGELDEKKETLAGRFVVGLATTGGVAQALLSAEENGFGPGYLDDYPRRVAALT
                     PDVVNRVVRERLDTSRLFVSIAGSVPG"
     gene            complement(3951..4589)
                     /locus_tag="AC16YU_12105"
     CDS             complement(3951..4589)
                     /locus_tag="AC16YU_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6066014.1"
                     /translation="MATRTRRSTVEEQPTHLFEDHNLDLESLSDDELEQLLFEEDEVS
                     TKGPLNLPTMAGLSLILVGIAYILQQMGIWSPLDLTALAAMLPWLAGILIILLGFGVL
                     SWRPRRRPRKRDRRKQAASIELEQPGPAKAKGRAGDRRKLHKSRDRKVSGVCGGLAEY
                     FGIDPTLMRIAFVIGTIVTQGTLVPAYIILAFVMPNPEKPTLEERITIIRDS"
     gene            complement(4636..5220)
                     /locus_tag="AC16YU_12110"
     CDS             complement(4636..5220)
                     /locus_tag="AC16YU_12110"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6066015.1"
                     /translation="MTHSKAQLRELLLARRQGLPPEVAAPASASISDRILALPELESA
                     RVVLAFWPMTSRREVDIRPALRTLAERGVVVGLPIIRPSDPARVMAFGTYASDEALAA
                     GPFGTLQPTGPGTLDGRAVDAVVVPALGATREGHRLGYGGGYYDRFLATTDAFTVIPL
                     YSVCLVDSLPIEAHDQAVDAVVTESGEFRAPRPT"
     gene            5294..5665
                     /locus_tag="AC16YU_12115"
     CDS             5294..5665
                     /locus_tag="AC16YU_12115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6066016.1"
                     /translation="MDQGQDRFAEFEAKRERLNRLVLQDHGHLGTRRFFALDSAAYRD
                     GALDGRTKELLGLVASTVLRCNDCIDYHLGRCVELGFGDDELDDALNVALVVGGSIVI
                     PHLRHAVESIGLLRAREERGD"
     gene            5810..>6591
                     /locus_tag="AC16YU_12120"
     CDS             5810..>6591
                     /locus_tag="AC16YU_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6066017.1"
                     /translation="MFDALSEKLDGALKSLAGQGRITELNVAQTMREIRRALLDADVN
                     FQVAKDFTERVRDRALGSEVLTSVSPGQLLVKIVYDELVHFLGEEHVGIEPAPVPPTV
                     ILVAGLQGSGKTTFCGKLALHFKSKGRAPMLAAADVYRPAAIDQLRTLAEQVGVPVYS
                     VEENGEPVRDAVRVAREALASARRQARDILIIDTAGRLHVDEPMMQEVQQIRDAVSPH
                     EILFVVDAMTGQDAVNTAVSFNERLDFSGVVLTKLDGDSRGGA"
BASE COUNT         1025 a         2216 c         2337 g         1013 t
ORIGIN      
        1 cgcgcagggc attcggggag tcgcgttcgg cggtggatgc gtctggatgg cgctgccggg
       61 ccgtatcgtg gccttttcgc ccggaggcgt cctggcatcc gactgggcga tcgagctcga
      121 tggtcccgtc gtggaccttg ccgtccggga cgaagggctc gtcctgctca ccgcccggtc
      181 gctctacgtg gcgtcggccg cctgcgcgcc ggagccctga tcaggccctg accgtcggag
      241 ccgcgccctt cctgccgagt tcgagcagcc gggcaagcgc ctgctccttg gcctcccggc
      301 tctcgacgga acgcagcagc acgggctcgg ccacgcgctc cgagcacgct tccggtgaga
      361 ggggacaccg ctcgcacgtc acgttcacct cctcgtcggg aacggccggg tcgtcccaga
      421 atcggacctt ctgccggaag ttgtcgtcga tcaggaaccc gagggtgatg ctggtgcggg
      481 cgcccgacga gagggtgagc ggatgcgaga gcgagaagac gaagaacgag acgccctccc
      541 gggtgaagtg ggatcgctgc gcgtgcacgc catactcgcc gctgcccccg tcctgctgca
      601 ggaggcggag gcccgaccac ctgcggcagt agtgctcgtc gagccagaag ccctgcggga
      661 gggcgatgcg cgaccggttg aagaccttgg tcagctcgac ccggtccgac tcggggcgga
      721 gggtcacccg aaggaagaag aactcctgga agcccgccat gtgcggcagg agctgcccga
      781 cccggtacag gaaagtctcc ggggtggtcc cgagccggtg gacggcctgg agcagggcgt
      841 ccccgtcgaa gctgcggcga cggaacaggt ggccgagcgt gtcgaggatg tcgtcgcggg
      901 caatgagcac ggccccggcg aagtacgagg cccggaagtt gttcaggacc tgttcgaacg
      961 actcgacctg cagccacgac gaggtgacgg cgcgttcccg caggcccagg agccggtacc
     1021 cgatctcgcg gccgtatacg aaggccttct gggacggcag gaggcgcggg tgcacgtaca
     1081 ggacgggctc cggcccatcg gcgaaaacgg atcggaagcc ggcgaggtcg gagcgctcct
     1141 cgagcatctc ttcgtccacc cggtacccga acgcctgctc caggtacgcg cggagcacgc
     1201 cttccgcgtc gccgtccacc gcctccggcc ccacctccgc acggaagccg tccgccacgg
     1261 cctcgatctc cgcgaaatag ttgtcgtgca tcagctggta ctgccggagc gccgcgaaca
     1321 ggaaatgctc gacccgcacg tcgtacatct ggccgatctc gaggaacgtc cggagcagtg
     1381 cgccggcccg tgccggatcg gacgagaaga ggctgaccag gtcctgggtg gccaggccga
     1441 acagctcgaa gggaaactcg cgaaagaagg gggaccggag ggtgtcggcg agcggatgga
     1501 gcgactcgtc cacgctcacg gacacgagct cgtcgaacgg cacctccagc atggtcgaca
     1561 gcgcgagcag cttgtcgggc ttcgggtact tcttgccctt ctcgatctcg ctgaggtagg
     1621 agatggacag ccccgaaccc gcggcaaggt ccttcaggga caagccccgg gcgtgcctga
     1681 ggttcttgag cttcaggccc aggatcagtc gtacgttctc ggcactgatc gacatgtccg
     1741 gcgaggtttc gctggcggcg aaagtaaggt ccgtcgcccc gccgatcaac gaaatcatgc
     1801 tggcagcgaa tcttcgctcg cgccccgaat ccgattcccg ggcgccgagg gccgtcgtct
     1861 cccgctccgt acagcgggat cagggaatgc ccgacaggcc ggcacccgga tggagccgac
     1921 tttcacggcc acaggttcga tttacctcag ggacgatgcg catcgctctc ttcgggccgc
     1981 caggtgcggg aaaaggcacc caggcggcgc tcatcggata ccgttacggc ttccgctcca
     2041 tatcgacggg cgagctcatc cggacggcca tgcgggacgg cacgccgatc ggaacggagg
     2101 cccgccgcta cgtggaggac ggaaccctgg tcccggacct cctggtgagc cggctggccg
     2161 aagcggcggt ggaggaatgc gacttcgacg gcttcatcct ggacggctat ccccggacgg
     2221 tcgggcaggc ccgctccctg gacgcgttcc ttgaccgcca cgatgcggcg ctcgacgcac
     2281 tgatctgcct gcacgtgccc gacgcggagc tcgtggaccg gctttccgcc cgccgggtgc
     2341 accggaagac gggcacgaca taccacctcg tctacaaccc gccgccgccc ggcgtcccgc
     2401 cggacctgct catccggagg cccgacgatc acccggacgc gatccggcgc cgcctctcgg
     2461 aataccggtc ggtaaccgag cccgtcatgg cctggtacgg cgaccacctc cgactcgttc
     2521 gggtggacgc gtccggaacg gtgtcggagg tggccttgcg cctcacgacg gtcctcgaac
     2581 gcctcgccgt ccgggccctg gcctgaccgg gctgcggttc gcgcggagcg gatcaccccc
     2641 gtaccttggg gcctccgaat ccagggcacc gcatgacgac gtacggcgag agagtcctcc
     2701 ggcaggaagt gggaccgtgc acgctcctcg tgctgcccac cggggcgaag gacgtggtgt
     2761 ccgtccggtt gtcgatcgat acgcatcccg acttcgcagc gggcgaggag ctggtccagg
     2821 acgtcacggc catgctgctc cagaagggga cccggcgccg cgaccggttc gaggtggcgt
     2881 cgatcctgga ggatcgcggc gcgcggctgt ctttctccgg ctccggcgta cgggtccggg
     2941 cgtccggccg gatgctccgg ggcgacgtgg gcgtgatcgt ccggctggcg ggcgagatgc
     3001 tcacggagcc ggcgttcgac gaggacgagc tctcgaagac gcgacttcgc gtgaagggct
     3061 ccgtcctccg atccgcgtcg gacacgggcc accgggccgc cgtcgagctg gcgcggcatg
     3121 ccctggagcc gggccatccc gggttcatgg cccgcccgga cgaggatctc gcagcgctgg
     3181 aatcgctcga cggacgggcg atccgggcct tccacgcgtc ccgattccgg ggtccggttc
     3241 gctgcgtcgc ggtcggggac gtggatgcgg cggccctccg ggaagcgatc gacgaggcct
     3301 tctccgcctg gccctccggg ccctcacgcg gcgtagcgta cccggattca ccggcgaccg
     3361 ttcccggcgc ggtccggacg ccgatcccgg accggccgaa cctggacgtg gtcctgggac
     3421 actcgctgcc cacgcggcgg gacgatcagg aattcgacgc cctgcacgcg ggggtattcg
     3481 ccctcggcgg caacttctcg gcccggctca tggcccacgt ccgcgacgaa ctgggcctga
     3541 cgtatggcat cggatcctcg ctgtcgggaa tcgggatcca ccacgggggg ctctggcaga
     3601 cgtcactgac gctctcggcg gaccgtctct ccgagggcat cgacgcgact cggcgcgagc
     3661 tggaccgctt cgtgtccgag ggcgtggcgc cgggcgagct cgacgagaag aaggagaccc
     3721 tggccgggcg cttcgtcgtg gggctcgcga cgaccggcgg cgtggcgcag gcgctgctct
     3781 cggccgagga gaacggtttc ggaccggggt acctcgacga ctatccgcgg cgcgtcgccg
     3841 cgcttacacc ggacgtggtc aaccgggtcg tccgcgaacg gctggatacg tcgcggctct
     3901 tcgtctcgat cgccggctcc gtgccgggct gatcccgcgc ccggaagccg tcagctgtcg
     3961 cggatgatgg tgatccgctc ctcgagcgtt ggcttctccg gattcggcat cacgaacgcg
     4021 aggatgatgt aggccgggac gagcgttccc tgcgtgacga tggtgccgat cacgaacgcg
     4081 atccgcatca gggtaggatc gatcccgaag tactcggcca ggcccccgca cacgcccgag
     4141 accttccggt cgcgcgactt gtgcagcttt ctccggtcgc cggcgcggcc cttggccttt
     4201 gccgggcccg gctgctcgag ttcgatcgac gcggcctgct tccgtcgatc ccgcttccgc
     4261 ggccgccgcc tggggcgcca cgagagcacg ccgaacccga ggaggatgat caggatgccg
     4321 gccagccagg gcagcatggc ggcgagcgcc gtcaggtcca gtggggacca gatgcccatc
     4381 tgctggagga tgtaggcgat ccccaccagg atgagcgaga ggccggccat ggtcgggagg
     4441 ttcaggggtc ccttggtcga tacctcgtcc tcctcgaaca gcaactgctc gagctcatcg
     4501 tcggacagcg attcgagatc caggttgtgg tcctcgaaca ggtgggtcgg ctgttcctcg
     4561 acggtcgatc gtctcgttct cgtagccatg gattaccggg tcggcccgtg gtgttacgcg
     4621 cggtgcaggg tgaggttacg tcggacgggg ggcgcggaac tcccccgact ccgtcacgac
     4681 ggcgtcgacc gcctggtcat gcgcctcgat cgggagggaa tctacgaggc agacggaata
     4741 aagggggatc accgtgaacg catccgtcgt ggcgagaaat cggtcgtagt agccgccccc
     4801 gtacccgagc cggtgaccct cgcgggtcgc tccgagtgcc ggcacgacga cggcgtcgac
     4861 agctctcccg tccagcgtcc cggggccggt cggctgcagg gtcccgaagg ggccggcggc
     4921 gagggcttcg tccgaggcat aggtcccgaa cgccatgacc cgcgccggat ccgacgggcg
     4981 gatgatgggg agacccacga cgacgccccg ctccgcgagc gtgcggaggg ccggccggat
     5041 gtcgacctcc cggcgggagg tcatcggcca gaacgcgagc acgacgcgcg ccgactcgag
     5101 ctccggaagc gccaggatcc ggtcggagat cgacgccgag gccggcgcgg cgacctcggg
     5161 cggcagtccc tgccggcggg ccaggagcag ttccctcagc tgggccttgc tgtgcgtcat
     5221 ggggtccgga tcgagaaggg aacggccggg ttggagggag gatcgacgtg gcattcaccc
     5281 atcttccgct gtaatggatc agggacagga tagattcgcc gagttcgagg caaaacgtga
     5341 acggctgaac cggctcgtgc tgcaggatca cgggcacctg ggcacccggc ggttcttcgc
     5401 cctcgattcg gccgcatacc gtgacggcgc gctcgacgga cgcaccaagg agctgctggg
     5461 gctggtggcg tcgacggtgc ttcgctgcaa cgactgcatc gactaccacc tggggcggtg
     5521 cgtcgagctc ggcttcggcg acgacgagct cgacgacgcc ctgaacgtgg cgctcgtcgt
     5581 gggcgggtcg atcgtgatcc cgcacctgag gcacgcagtc gagtcgatcg gcctgctgcg
     5641 ggcgcgtgaa gaacgtgggg attgagggga gcggcatcac gcggcgcccg ggccggtgcg
     5701 gccggggagg cggggcgatt gtggaagcca cgattgattc gtactttttc ctgtctgtac
     5761 ccattcagcg gatccaccag gaatccgcgc acccggccgc cccgcgatca tgttcgacgc
     5821 cctgtccgaa aagctcgacg gcgccctgaa atcccttgcg ggacaggggc ggatcaccga
     5881 gctgaacgtc gcgcagacga tgcgcgagat ccggagggca ctgctcgacg cggacgtcaa
     5941 cttccaggtg gccaaggact tcacggagcg ggtgcgcgac cgcgccctcg gctccgaggt
     6001 cctgacgtcg gtctcgcccg gccagctcct cgtcaagatc gtctatgacg agctggtcca
     6061 cttcctgggc gaggagcacg tgggcatcga gcccgcgccc gtcccgccga ccgtgatcct
     6121 ggtggcgggc ctccaggggt cgggcaagac cacattctgc ggcaagctgg ccctgcactt
     6181 caaatcgaag ggccgcgccc cgatgctggc tgctgccgac gtctaccgtc ccgcggcgat
     6241 cgaccagctg aggacgctcg cggaacaggt gggcgtgccg gtgtattcgg tcgaggagaa
     6301 cggagagccg gtgcgcgacg ccgtacgcgt cgcccgtgag gccctcgcct ccgcccggcg
     6361 ccaggctcgc gacatcctga tcatcgacac ggccggccgt ctgcacgtgg acgaaccgat
     6421 gatgcaggag gtgcagcaga tccgggacgc ggtcagcccg cacgagatcc tgttcgtcgt
     6481 cgatgccatg acgggccagg acgccgtgaa cacggccgtc tcgttcaacg agcgcctgga
     6541 cttcagcggt gtcgtcctga ccaagctcga cggcgactcg cgcggcggcg c
//
LOCUS       DBNHQZ010000447         4066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_3385695, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000447 DBNHQZ010000000
VERSION     DBNHQZ010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_3385695"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16YU_12125"
     CDS             complement(<1..251)
                     /locus_tag="AC16YU_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HIR6066018.1"
                     /translation="MIPPTILPRLQVATDTLRESGMTTAYDSIWGAGNAVPTQPPGPV
                     EQVMLSDDKIFVVLAVVLIIWIGISIFLLRTDRKLDSLER"
     gene            complement(271..981)
                     /gene="ccsA"
                     /locus_tag="AC16YU_12130"
     CDS             complement(271..981)
                     /gene="ccsA"
                     /locus_tag="AC16YU_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR6066019.1"
                     /translation="MSPHRAAVDVRRYRIVRNVVLAWLTAMILAAFLFQIPRINILEH
                     TARNLYFHVPMWFTMMAAVFVSAWHSLQVLRGGGLIRDIRALQAARVGATFGVMGLVT
                     GIVWSKYTWYVGTDVWWNFDPRQTMAALQLLIYGAYFVLRSAVDEPGRRARVAAVYNL
                     FAAVTVPFLLYVLPRQLQSLHPGADGNPAFSEITHPIMRLVFYPAVIGFIGLFWVLYT
                     QRVRLAAAKLRLTRDPEL"
     gene            complement(1051..1722)
                     /locus_tag="AC16YU_12135"
     CDS             complement(1051..1722)
                     /locus_tag="AC16YU_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6066020.1"
                     /translation="MNWIHGTWAVLEKDLRLEMRSRYAVNMLLMFVLSSLLLVAFAVG
                     QDEISPRLRAALLWIVILFSASIGLGRAFVAEEERGTVLLLRLNVRGSMVYAGKLAFN
                     FLLLLAVDIVATAVVALILDLDVAVPGLLAAVLALGSLGLAGATTLLAAIIARASNRG
                     PLLPVLLFPILVPLLLSVVSGTREALEGGTGWASAGDELATLAGFAGVIITTAVLLFD
                     FVWDD"
     gene            complement(1739..2680)
                     /gene="murB"
                     /locus_tag="AC16YU_12140"
     CDS             complement(1739..2680)
                     /gene="murB"
                     /locus_tag="AC16YU_12140"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6066021.1"
                     /translation="MEAERQELTERIGRHRVGRGVPLAPYTTFRIGGPAALYVEAATT
                     RELVLALSAARELGIPHFLLGMGANILIGDGGFDGLVVRNATRRVAVDPALGLLTAES
                     GAVMYPDVIGTAVAAGLSGIEHYVGIPSTVGGALWQNLHFLSPPPERTRTMFIAEVLQ
                     GASILGEDGDVRSVDTAYFAFGYDYSVLHDRDDVVLSATFRLEPGDPSVLRRTMAANL
                     EWRRERHPPLDTEPSAGSVFKKIDGVGAGRLIDGCGLKGHRVGGAEVSPRHANIFVNR
                     GGATAADVRSLIEYVQGVVERETGYRLEPEIAFVGTF"
     gene            2870..3706
                     /locus_tag="AC16YU_12145"
     CDS             2870..3706
                     /locus_tag="AC16YU_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIR6066022.1"
                     /translation="MAQNPYPNVTRALARFAVLGVVLLIVLGMAGGCMTTSIRSGEGA
                     VKYSVFGGTDLEARFGEGLQVHAPWVDLIRYDLRVQEQLEDITALSSNGLQIGMDASI
                     RWRPRAEDLPELHTTYGIDYYRKLVQPELRSAVREIVGQYTPEELYSSRRTELQQQVF
                     QRVRDAVESNYVQVDAILIRDVSLPEQIRGAIENKLKEEQEAERYQFTIQKERLEAER
                     KQIEAQGQAEYQRIITASLSEQFLRYKGIEATQMLANSPNAKTVVVGGGRDGLPLILG
                     GQ"
     gene            complement(3745..>4066)
                     /locus_tag="AC16YU_12150"
     CDS             complement(3745..>4066)
                     /locus_tag="AC16YU_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6066023.1"
                     /translation="EVYDRMLALCPLEDDVLLWNERGELTESCFANVFLERDGRLLTP
                     PARSGLLPGTLRGRLLADRRAVEAVLTPADLADGRVVLGNSVRGLYAAASVTDRRVRR
                     PLEV"
BASE COUNT          710 a         1385 c         1413 g          558 t
ORIGIN      
        1 cgttccagcg aatcgagctt gcggtccgtt ctcaacagga agatcgaaat cccgatccag
       61 atgatgagca cgaccgcgag caccacgaag atcttgtcat ccgagagcat gacctgctcc
      121 acgggcccgg gcggctgggt cggaacggcg tttccggcac cccagatgct gtcgtaggcg
      181 gtggtcatgc ccgactcccg gagggtgtcc gtggcgacct ggaggcgcgg aagaatcgtc
      241 ggcgggatca tcgtcggaag ggttgtgggc tcagagctcg ggatcgcggg tgaggcgcag
      301 cttcgcggcg gccagccgga cgcgctgggt gtacagcacc cagaacagcc cgatgaatcc
      361 gatcacggcc gggtagaaga ccagccgcat gatggggtgc gtgatctcgg agaaggcggg
      421 attgccgtcc gccccggggt gcaggctctg cagctgccgc ggaagcacgt acaggaggaa
      481 cggcacggtc acggcggcga acaggttgta caccgcggcc acgcgggccc ggcgcccggg
      541 ctcgtccacg gccgagcgga gcacgaagta cgccccgtag atcaggagct ggagggccgc
      601 catggtctgg cgcggatcga aattccacca cacgtcggtc cccacgtacc acgtgtactt
      661 ggaccagacg atgcccgtca cgagccccat gaccccgaac gtcgcgccga cccgggccgc
      721 ctgcagcgcc cggatgtccc ggatcagccc gccgccgcgg agcacctgca gggaatgcca
      781 cgcggacacg aagacggccg ccatcatcgt gaaccacatg ggcacgtgga aatagaggtt
      841 gcgcgccgtg tgctccagga tgttgatccg ggggatctgg aacaggaacg ccgcgaggat
      901 catcgccgtg agccaggcga ggaccacgtt ccggacgatc cggtatcggc gtacgtcgac
      961 ggcggcccgg tggggggaca tggcgaatcg ggctgggggc agttggcggg gcggcaaaga
     1021 taatggccgg gcgggcccgg cgcatcccat tcagtcgtcc cagacaaaat cgaaaagcag
     1081 gaccgccgtc gtgatgatga cgcccgcgaa cccggcgagc gtcgcgagct cgtcgcccgc
     1141 ggaggcccac ccggtgccgc cctcgagcgc ctcgcgcgtg ccggagacga ccgacaggag
     1201 caggggcacg aggatgggga acagcagcac cggcaggagc ggtccgcggt tcgatgcgcg
     1261 cgcgatgatc gccgcgagga gggtcgtggc gccggcgagt cccagcgatc ccagcgcgag
     1321 caccgcggcc agcagccccg ggacggccac gtccaggtcc aggatcaggg cgacgaccgc
     1381 ggtggccacg atgtcgacgg cgaggagcag caggaagttg aacgccagct tcccggcgta
     1441 gaccatcgat ccgcgcacgt tcaggcggag gagcagcacc gtgccccgtt cctcttcggc
     1501 cacgaaagca cgtccgaggc cgatggacgc cgagaacagg atcacgatcc agaggagcgc
     1561 cgcccgaagc cgcggggaga tctcgtcctg tcccaccgcg aaggccacga ggagcagcga
     1621 cgacaggacg aacatgagca gcatgttcac ggcgtaccgg ctgcgcatct ccaggcgcag
     1681 gtccttctcg agcacggccc aggttccgtg tatccagttc atgtggcgaa tcggcggttc
     1741 agaacgtccc tacgaaggca atctcgggct cgagccggta cccggtctcg cgctcgacga
     1801 ccccctgcac gtattcgatc agacttcgaa cgtccgcggc cgtggcccca ccgcggttca
     1861 cgaagatgtt cgcgtgccgt ggcgacactt cggcgccgcc cacccggtgc cccttgagtc
     1921 cgcacccgtc gatcagtcgc ccggcgccca cgccgtcgat cttcttgaac acggatccgg
     1981 cgctcggctc cgtgtccagc ggggggtgcc gctcgcggcg ccactccagg ttcgcggcca
     2041 tcgtccggcg gagcaccgac gggtctccag gctcgaggcg gaacgtggcc gagaggacca
     2101 cgtcgtcccg gtcgtgcagc accgagtagt cgtagccgaa ggcgaagtac gcggtgtcga
     2161 ccgaccggac gtccccgtcc tcgccgagaa tggaggctcc ctggagcacc tcggcgatga
     2221 acatggtccg cgtcctttcg ggcggaggcg acaggaaatg gaggttctgc cagagggcgc
     2281 cgccgaccgt cgacggaatg cccacgtaat gctctatgcc ggaaaggccg gccgcgacgg
     2341 ccgtgccgat gacgtcggga tacatcacgg cgccgctctc ggccgtgagg aggccgagcg
     2401 cggggtcgac cgccacgcgc cgcgtggcgt tccggacgac gaggccgtca aagcctccgt
     2461 ccccgatcag gatgttcgcg cccatcccga gcaggaaatg cgggatgccc agctcccggg
     2521 cggccgacag cgcgaggacg agctcccgcg tcgtggccgc ctccacgtag agcgccgccg
     2581 ggccgccgat gcggaacgtc gtgtagggag ccagcggcac tccccgcccg acgcgatggc
     2641 gtccgatccg ttccgtcagc tcctgccgct ccgcctccat gggcctgcgc gctccgtcgg
     2701 gtgtcgtgtc cgcccgcgtg ccgctccggc gcggccgctt cggcggcggt cgccaatata
     2761 cgccgggtcc gtgccccgta ttctcgccga atgaaccgtc cggcgtgcaa cccggcgggc
     2821 cgagtccggt acgacggcac tgcaacggat caaacgacag gcgatcgaaa tggcccagaa
     2881 tccatacccc aacgtgactc gtgcgctcgc gcggttcgcc gtactcggcg tcgtcctcct
     2941 gatcgtcctc ggcatggccg ggggctgcat gacgacctcc atccgctccg gagagggcgc
     3001 cgtgaagtac tccgtcttcg gtgggaccga cctcgaagcc aggttcggag agggactgca
     3061 ggtccacgct ccctgggtgg acctgatccg gtacgacctg cgcgtacagg agcagctcga
     3121 ggacatcacg gcgctttcgt cgaacggcct gcagatcggc atggacgcat ccatccgctg
     3181 gcgcccgcgg gccgaggatc tgcccgagct gcacaccacg tacggcatcg actactaccg
     3241 gaagctcgtc cagccggagc ttcgcagcgc ggtgcgcgag atcgtgggcc agtacacgcc
     3301 cgaagagctc tactcctcgc gccggaccga gctgcagcag caggtattcc agcgggtgcg
     3361 tgacgccgtg gagtccaatt acgtgcaggt ggacgccatc ctgatccggg acgtgtcgct
     3421 tcccgagcag atccgcggcg cgatcgagaa caagctcaag gaggagcagg aggccgagcg
     3481 ctaccagttc acgatccaga aggaacggct cgaggccgag cgcaagcaga tcgaggcgca
     3541 gggccaggcc gagtaccaga ggatcatcac cgccagcctt tccgagcagt tcctgcgcta
     3601 caagggcatc gaggcgacgc agatgctcgc caactcgccc aacgccaaga ccgtggtcgt
     3661 agggggaggc cgggacgggc tgccgctgat cctgggcgga cagtagtggc cctgccggcg
     3721 ccctgggctc ggcggccgcg ggtctcagac ttccagcggc cgccgaaccc gccgatccgt
     3781 gacggacgcg gccgcgtaca gcccgcggac cgagttgccg agcacgacgc gcccgtcggc
     3841 caggtcggcc ggcgtgagca cggcctcgac ggcccttctg tccgcgagca ggcgcccccg
     3901 gagcgttccg ggcagcaggc ccgagcgcgc gggcggggtg aggagccgcc cgtcgcgctc
     3961 caggaacaca ttggcgaagc agctctcggt gagttcgccc ctctcgttcc agagtagcac
     4021 gtcgtcctca agcggacaca gcgccagcat gcggtcgtag acttcc
//
LOCUS       DBNHQZ010000448         6612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_268427, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000448 DBNHQZ010000000
VERSION     DBNHQZ010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_268427"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1005)
                     /locus_tag="AC16YU_12155"
     CDS             complement(121..1005)
                     /locus_tag="AC16YU_12155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR6066024.1"
                     /translation="MIASMTGFGRGQVEVDGRSATVEIRTVNGRFCEVSVRSPRDLSE
                     YEGEITRRVKDTLARGRVSVSVQVDAADRPAVGLAVDLEMVRGYRALLDDLRKAAGLD
                     GETVKLEHLLRFQELFTPPSEESDAPAQAWVVVQQALDAALEALTAMRRQEGLALRAE
                     LLLRAERLEEGLAVVENRVPLRIPEIRDRLRDRLQELLQDARVDSDRLEQEMAYVADR
                     LDVTEECVRLHSHLALFREALDSPEPVGRKLNFLAQEMNREVNTIGSKANDTEVAHRV
                     VGMKEDLEKIREQVENVE"
     gene            complement(1082..2929)
                     /locus_tag="AC16YU_12160"
     CDS             complement(1082..2929)
                     /locus_tag="AC16YU_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066025.1"
                     /translation="MRSALLLLFLTIAAPVMAQTPRYDHFDAAIYARAYEVREMADLD
                     WLRERFDVMAGHVRVSKVYLETHRDMILVSGETLEKAKAFFAERGIRTSGGITLTVDE
                     SNRFETFCYSNPEHRAWVRQVVEHTARHFDEVILDDFFFTSCKSEGEIAAKGDRSWTA
                     YRLALMDEAARELVLGPAKAVNPDVEVVIKYPNWYAHFQGLGFNLETEPAIFDGLYTG
                     TETRDAVRSAQHLQPYLGYDVFRYFENLKPGGNRGGWVDTGGMRTLDRYAEQLWVTLF
                     AKAPEITLFDFRQMQRPLLAGHRAPWKGSLDFDAVVAPHILGDGSIDPSLTLARAAGW
                     ALAKVDPVLAALGNPVGIPSYRPFHATGEDFLHDFLGMIGLPIDLRSEFPADAPTVLL
                     TEYAAADPNIVSRIRERLVAGGNVVITTGLLRALADRGIRDIAEIEVTGRRALVEDFI
                     AGRGDPIRGRKKILIPQVHYLTNDSWELVSAVDGPMGWPILHDADYAAGHLFVLTIPD
                     NPADLYALPDAVLDRIRQVVTEDFFVRVDGPSEIALFAYDNDAFVVESFRDGASEIRI
                     VTDGRLTRLRDVESGEILDGAPVEQGPDAGRRVFATGIGPHAFRAFAAE"
     gene            3045..3230
                     /locus_tag="AC16YU_12165"
     CDS             3045..3230
                     /locus_tag="AC16YU_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066026.1"
                     /translation="MADRVRRVGELMLRHHDVPMSFADACLVCMAEVEDSAIFTLDRD
                     FSEYRRGRNDPLRLIMP"
     gene            complement(3575..4636)
                     /locus_tag="AC16YU_12170"
     CDS             complement(3575..4636)
                     /locus_tag="AC16YU_12170"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HIR6066027.1"
                     /translation="MYSSPTAGRDAQPFRHEGRHRAAQTLLFALAPLLLATCRPVETL
                     PARLEDVPAQAHADRTLRELEILSADTVATDIGWVRGIRTDDEGRLYVHDHSEQQIVV
                     LDSTGTRLRTLGRRGAGPGEFEFISGFDVRGDGTVVVADGQLSRLTVFGSDGAVLASR
                     GMDGRTHGIAATAKAFVVFDPLARAAFRVLSDSLADGHVVPLIADFDAGGDPMEQLRR
                     AEGLVDGVAAGYVLVTMYAGMILVYDSEGRLTAARRTIDGPALSEVARALDTEMTEGV
                     AFSMLVNATDTEIHVGSTAQADPFLVDVYRRDDARYSHSFEIPHPECGPRYVDERVIV
                     SGCADGPVLRTVYRVVSHD"
     gene            complement(4935..5855)
                     /locus_tag="AC16YU_12175"
     CDS             complement(4935..5855)
                     /locus_tag="AC16YU_12175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6066028.1"
                     /translation="MNLRISALLTVLLVLAGSAAAQDGTIYPLDAPAEPAAIPLGTGG
                     VEGQTAPESWFRQWGDPMARNVSIATLTPFLPEPGKANGAAVIVAPGGGFMWLSMGNE
                     GWEVAEALAEQGVAAFVLKYRLRPTAASLDDFRDSMNRPPSPPPSDTTQVAGPPRPPR
                     WDLSDQLEDAEAAYAMIVDRAEEWGVDTQRIGMIGFSAGAGLTMHSTLNSQTMELAFI
                     GPIYGGMGPVEVPEDAPPMFSVIASDDFLFRGQFGVVESWFAAGIPVEFHLYQNGGHG
                     FGLGNPDRTSNRWFDAFLHWLDVNGFLAAE"
     gene            complement(6110..6397)
                     /locus_tag="AC16YU_12180"
     CDS             complement(6110..6397)
                     /locus_tag="AC16YU_12180"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6066029.1"
                     /translation="MSPSIDDIRRKVVDLMRERGVTRAGVFGSFARGDENDSSDVDFL
                     VEFESGRSLLDLSGLRLDLAAALDREVDVATPDSLHPRLRERVLAELVPLL"
BASE COUNT         1098 a         2191 c         2270 g         1053 t
ORIGIN      
        1 ggtgaccggg tggtccccga cacggagaag cgcatttcgg gcacggcctc gagcagccgc
       61 cgcgcgatgc tcgtcttgcc cgagccgctg ggcgcggtga ggaccacgac gaccgggccg
      121 ctattcgaca ttctcgacct gctcgcggat cttctcgagg tcctccttca tgccgacgac
      181 gcggtgcgcc acctcggtgt cgttggcctt cgagccgatc gtgttcacct cgcggttcat
      241 ttcctgcgcg aggaaattca gcttgcggcc cacgggctcg ggcgagtcga gggcctcgcg
      301 gaagagcgcg aggtgcgagt ggagccggac gcactcctcg gtgacgtcga ggcggtcggc
      361 cacgtaggcc atttcctgct ccaggcggtc gctgtccacg cgggcgtcct gcaggagctc
      421 ctgcagccgg tcacgcagcc ggtcacggat ctcggggatg cggagcggga cccggttctc
      481 gacgaccgcg agcccctcct ccaggcgctc ggcccggagc aggagctcgg cgcgcagcgc
      541 cagcccttcc tgcctgcgca tcgcggtcag ggcctcgagg gcggcgtcga gcgcctgctg
      601 cacgacgacc caggcctggg ccggggcgtc ggattcctcg gagggcggcg tgaacaactc
      661 ctggaagcgg agcaggtgct cgagcttcac ggtctccccg tcgagaccgg cggctttgcg
      721 caggtcgtcg agcagggccc ggtagccgcg gaccatctcc aggtcgaccg cgagccccac
      781 cgccggccgg tccgccgcgt ccacctgcac ggagaccgac acgcgcccgc gggccagcgt
      841 gtccttcacg cggcgcgtga tctcgccctc gtactcggac aggtcgcggg gcgagcggac
      901 agagacctcg cagaaccggc cgttcaccgt gcggatctcg accgttgccg aacggccgtc
      961 gacctcgacc tgaccccggc cgaagccggt catgctggcg atcatgtgcg cgtggagctg
     1021 tgggggcctc ggaacggcgg aaaatacggc gcggcggggc gggatgcggg gatggggcgg
     1081 ctcactcggc cgcgaacgcc cggaaggcat gggggccgat gcctgttgcg aatacgcgcc
     1141 ttcccgcgtc cggaccctgc tcgaccggcg ctccgtcgag gatctccccc gactccacgt
     1201 cgcgcagccg cgtgaggcgt ccgtccgtca cgatccggat ctccgacgcc ccgtcgcgga
     1261 aggactccac cacgaaggcg tcgttgtcgt acgcgaagag ggcgatttcc gagggcccgt
     1321 ccacgcggac gaagaagtcc tccgtcacga cctgtcggat ccggtcgaga acggcgtccg
     1381 ggagggcata gaggtccgcc ggattgtcgg ggatcgtgag gacgaagagg tggcccgcgg
     1441 cgtagtccgc gtcgtgcagg atgggccagc ccatagggcc gtcgacggcc gagacgagct
     1501 cccacgagtc gttcgtgagg tagtggacct gggggatgag gatcttcttc cggcccctga
     1561 tcgggtcccc ccggcccgcg atgaagtcct cgacgagggc ccgacggccg gtgacctcga
     1621 tctctgcgat gtcgcggatg ccgcggtccg cgagcgctcg gaggagaccc gtggtgatga
     1681 cgacgtttcc gcccgccacg agccgctccc ggatgcggct gacgatattc gggtccgcgg
     1741 cggcatattc ggtgaggagc accgtgggcg cgtcggctgg gaattcggac cgcaggtcga
     1801 tcggcagccc gatcatcccc aggaagtcgt gcaggaagtc ctcgcccgtg gcgtggaagg
     1861 ggcggtagga ggggatgccg acgggattcc cgagggcggc gagcacggga tcgaccttcg
     1921 cgagcgccca gcccgcggcg cgcgccagcg tgagcgaggg atcgatcgag ccgtcgccca
     1981 ggatgtgcgg cgcgacgacg gcgtcgaagt ccaggctgcc cttccacggc gcccggtgcc
     2041 cggcgaggag cggccgctgc atctgccgga agtcgaagag cgtgatctcg ggggccttcg
     2101 cgaagagcgt cacccagagc tgctcggcgt accggtccag cgtgcgcatg ccacccgtgt
     2161 ccacccagcc gccgcggttg ccgcccggct tcaggttctc gaaatagcgg aagacgtcgt
     2221 agcccaggta cggctgcagg tgctgggcgc tccggacggc gtcgcgtgtc tcggtccccg
     2281 tgtacaggcc gtcgaagatc gcgggctccg tctccaggtt gaatccgagt ccctggaagt
     2341 gcgcgtacca gttggggtac ttgatgacga cctccacgtc ggggttaacg gccttcgcag
     2401 ggccgaggac cagctcgcgg gccgcctcgt ccatgagggc gaggcggtac gccgtccagc
     2461 tccggtcgcc cttcgccgcg atctcccctt ccgacttgca gctcgtgaag aagaagtcgt
     2521 ccaggatcac ctcgtcgaaa tgccgggcgg tgtgctcgac gacctgccgc acccacgcgc
     2581 ggtgctctgg gttcgagtag cagaaggtct cgaagcggtt gctctcgtcg accgtcagcg
     2641 tgatgccgcc cgacgtccgg atgccgcgct cggcgaagaa cgccttggcc ttctccagcg
     2701 tctcgccgct gaccaggatc atgtcgcggt gggtctccag gtagaccttg gagacgcgca
     2761 cgtggcctgc catcacgtcg aaccgttcgc ggagccagtc gagatcagcc atctcgcgga
     2821 cttcgtaggc gcgggcgtag atggcggcgt cgaagtggtc gtatcgcggg gtctgtgcca
     2881 tgactggggc ggcaatcgtc aggaagagca ggaggagggc ggagcgcatc gcggcatccg
     2941 gatgtcgacg agtgcacgga gtgtacgacg caggcggatt ccctaatccc gggcggatcg
     3001 ggagccggag gttacggcga cgcacacgcg acgctctaac ggaagtggcc gatcgcgtgc
     3061 gcagggtggg cgagctgatg ctgcgccatc acgacgtgcc gatgtcgttc gccgacgcct
     3121 gtctcgtctg catggccgaa gtcgaggaca gcgcgatatt cacgctcgat cgggacttca
     3181 gcgaatatcg gcgcggcagg aacgatccac tccggctgat catgccttga gggggaggcg
     3241 gtccggaatg gctggccaga ggcttgagtg gaggcccgtc gacacgtcgg gttgactcgt
     3301 cgttggcaac gacatgctcc acagacctgt cgaaatagtc aagaaatatc gacaggtcat
     3361 caagtacggc ctgctcggcc gggaatagcg gtcatcgact ggcggttcct cgaactgtcg
     3421 acgacggata cgaacgaggt tgcgccgccg ggataggatg gagccatgga gacctcagca
     3481 agaagcggct cctgcgccct ccacgcacgc gaatgggcgt acaccgactt accgcccgtc
     3541 cgccctggtg acacccgtcc gagccggcgg gacgtcagtc gtgcgagacc acgcggtaca
     3601 cggtcctcag cacgggcccg tccgcgcacc cggatacgat cacgcgctcg tcaacgtatc
     3661 gcgggccgca ctccggatgc gggatctcga acgaatgcga atacctggcg tcgtctcggc
     3721 ggtacacgtc cacgagaaac ggatcggcct gcgcggtgct gcccacgtgg atctcggtgt
     3781 ccgtcgcgtt gacgagcatg ctgaacgcga cgccctcggt catttcggta tccagggcgc
     3841 gagccacctc cgagagggcc ggcccgtcga tggtccggcg cgcggcggtg agtcgtcctt
     3901 ccgagtcgta gaccaggatc atcccggcgt acatcgtgac gagcacgtac cccgccgcga
     3961 cgccgtcgac caggccttcg gcgcgccgga gctgctccat ggggtcgccg cccgcgtcga
     4021 aatccgcgat gagcgggacc acatgcccgt ctgccaggct gtccgagagc actcggaagg
     4081 ctgccctcgc cagggggtcg aacacgacga acgccttcgc ggtcgcagcg attccgtgcg
     4141 tgcggccgtc catgccgcgc gacgccagca cggcgccgtc cgatccgaac acggtcagcc
     4201 tcgacaactg gccgtccgcc acgaccaccg tcccgtcgcc ccggacgtcg aatcctgaaa
     4261 tgaactcgaa ttcgccgggt cccgcacccc tgcggcccag ggtacgaagc cgcgtacccg
     4321 tggaatcgag caccacgatc tgctgctcgg agtggtcatg gacgtacagt cttccttcgt
     4381 cgtccgtccg gataccgcgc acccaaccga tgtccgtcgc gaccgtgtcg gccgagagaa
     4441 tctcgagctc ccggagggtc cggtcagcgt gcgcctgcgc agggacgtcc tcaaggcgcg
     4501 cggggagcgt ctcgacgggg cggcaggtgg cgaggaggag aggcgcgagc gcgaaaagaa
     4561 gcgtctgcgc cgcgcgatgg cggccctcat gtctgaaggg ctgtgcgtca cggcccgcag
     4621 tcggtgacga gtacatcgtg ccagttgacg aggcgggagg actcaaggcc ggccggcatc
     4681 tccttcgccc gtgggcgacg caatgtacga cgccatccga ccttccggtc gacgaggctc
     4741 ccggattcgc gcccgtcgga agcgcctcgc ggcgcaccat ccgagtctcc agcaaggact
     4801 cgcgcggttc gagcgttggg ccgggatccc gggaggcaac gtgcgattcc catcaggtgc
     4861 ccattgcagc ccgaattcac ggcaagactt cgcctaagac actgtccggt tcttccgggt
     4921 ccgttcagcc tgcctcactc ggcagcgagg aagccgttca cgtccagcca atggaggaag
     4981 gcatcgaacc agcggttgct ggtgcggtcg ggattgccga ggccgaagcc atgaccgccg
     5041 ttctggtaca ggtgaaactc cacgggaatc ccggcggcga accaggactc gaccacgccg
     5101 aactgaccgc gaaagagaaa gtcatccgat gcgatgacgc tgaacatggg cggtgcgtcc
     5161 tccgggacct cgaccggtcc catccccccg tagatcggcc cgataaaggc cagttccatc
     5221 gtctgggagt tgagcgtcga gtgcatggtg agaccggcac cggccgaaaa gccgatcatc
     5281 ccgatacgct gcgtgtcgac accccattcc tcggcccgat ccacgatcat ggcgtacgcg
     5341 gcctcggcat cctccagctg gtccgacagg tcccagcgcg gcggtctcgg aggccccgcg
     5401 acttgcgtcg tgtcggaggg cggtggcgac ggcgggcgat tcatcgagtc gcggaaatcg
     5461 tcgagcgatg cggcggtcgg gcggagccgg tacttgagca cgaaggcagc caccccctgc
     5521 tccgcaaggg cctccgcgac ctcccagccc tcgtttccca tggacaacca catgaagcct
     5581 ccaccgggag ccactatgac ggccgcgccg ttcgccttgc ccggttcggg cagaaagggc
     5641 gtgagcgtcg cgatcgagac gttgcgagcc atggggtccc cccactgccg gaaccagctc
     5701 tcgggcgcgg tctggccctc cactccgccc gtcccgagcg gaatggccgc gggctcggca
     5761 ggggcgtcga gcggatagat ggtcccgtcc tgcgctgcgg cggaacccgc aaggaccagc
     5821 aggacggtca ggagggctga aatacggagg ttcatgggaa cacctgtcgg cagaggggtg
     5881 atgcaatgaa cgggaagtcg tcgatcgaag aacgaggagg ctccaacccg cggatgtcaa
     5941 gctacgacac acagtccaag gcggaggctt cctcgtcgcg ggacggattg aacggcgacc
     6001 tgctgttcgg tgccgttcga gaacccctct aattcgacac cgtcaacata tacctcgacg
     6061 agccgtatgg cttccttgat ctcgcgcagc agaatgtcag ccgagcgagt catagtagtg
     6121 gcacgagctc tgcaagcacc cgctcccgaa gtcgcggatg aagtgagtca ggcgtcgcca
     6181 cgtcaacttc gcgatcaagc gccgccgcga gatcgagcct caaccccgag aggtccaaca
     6241 ggctgcgacc gctctcaaac tccacgagga agtctacgtc actggagtcg ttctcgtcgc
     6301 cgcgggcaaa cgagccgaac acaccggcgc gcgtgacccc gcgctcgcgc atcaggtcaa
     6361 ctaccttgcg gcggatatcg tctattgatg gactcatgag atccagtacg acgcccgttt
     6421 taggacccgg ttcagattgg cgcttccgtc acggtgcctt ctaacccttc ctgcacggac
     6481 gtccgcctgt cgcgggtggg attccgctcg ctttgctcga tgggtatccc gcggggggag
     6541 gtcctgcacg gacgtccgcc tgttacgggc gggattccgc tcgctttgct cgctgggtat
     6601 cccgcggggg ga
//
LOCUS       DBNHQZ010000449        15020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1217173, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000449 DBNHQZ010000000
VERSION     DBNHQZ010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 15020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15020
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1217173"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2827
                     /locus_tag="AC16YU_12185"
     CDS             <1..2827
                     /locus_tag="AC16YU_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6066030.1"
                     /translation="VLGERLIRQDEVSTTTVMRGEEIQALPVDNFQDAISLAGGVVEN
                     AAGQDPGIHVRGGRTSEIAYLVDGVLVQDPLSGSLGGFDVARQSIDELQVLTGGFSAK
                     YGKAMSGIILVNTPAGGNEYHGALRLQTDGLSMEGVGQGGPALDYRRPFSNDWGTRSV
                     EGTISGPIVKDRVTFFLNADRLDSDSYLSEFEGPVRPAMLPWSQLEGLTFLRPGTTSL
                     IDANGAPVELDLETLGIEEGTVVTAEMIEDLREAGVDVDRQAKAAVSDYRQDHQLGLY
                     DDRTRFVANLGIKVNDDIDTRLGYKLTRREWRTYDHGFKYLPQYNDKSQREIDVFSAQ
                     VTHRVASNMFYELRASYTGNRYYEFLYDESKDENDPWRFGRLFSPYSVDGAFNTEGLA
                     DAEGGNNYDFLGYSEVGIYVDYPYLLTGDLDQPFQLSRNLDLDGDGTVDHFEGDRLTE
                     DLVQELKDLYATLSDEEIEAMGLSKNLIPVLAPPTERDYEERNIKDFQLGLDFTAQVD
                     DRNMLQAGVEFRKMSLFRYLILANTYWDPQTDPSLETTLTHRPEDEFYVDVKPFEVAA
                     YVEDKIEFGSLVMRPGLRLDVLDPDMATLADYGQVTVMQRPSQPNDPRPETVQASVKW
                     RISPRFGIAFPLTDRARLSFNYGQFLQYPEYNRLYESYRTAAWIPGDGYVPLEEFGFD
                     LGFESFIGNPDIKPEKTIFYQVDGEFLVSESFKLGASLFYKDIYDYVAVSRTLTQGGQ
                     FVWVMDNLDYANSRGFEVRAEKRLEEYLGFSASYTYSRSVGNADDYAGRYDDWYNNSV
                     AGTIPAKKAEPLDWDQPHTLGFMVNGKYSGLKGSLLGRFGSGLPYTPTTTRGLPLGPK
                     NSARRPWTGSIDARLEYRFDLPRYGWITPFFEVTNLLDRKNVLAVYSDTGSPDFTLDP
                     GTQYEDAQRPNLIGPPRHFEVGFTLGF"
     gene            2915..6004
                     /locus_tag="AC16YU_12190"
     CDS             2915..6004
                     /locus_tag="AC16YU_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR6066031.1"
                     /translation="MKRPVNIQGRPLAILTGLLVTWAGLAFSARAQQGMTKAQYMDYL
                     TRSGRIEYPAGPIIAGKTLGPADLKLSVLDIGNVRARIRNTGTLGYDRDLLCYEFPFN
                     SGITYRWTLGPMIAGRIDGQKYVSGAALGAVRGTTEDEFRPLYGFDSGDYRSELNIGI
                     AFSDKPASWPSSWPTIDEALADNGQHILNPAARTAFLANPTFDPVATGTARIGSKGFP
                     GVADGEIRAPREAYFVITDNDPAQPAAPLPMNVRVDVWAMQWDDFINRNFIIYKMVFT
                     NIGEKTIENVYIGIHDDPDAPEQGSNEWTDDYAYLVPPGKDTDGDGVPDPDADPRADV
                     DGDGMYTGEDSLLWNTVYLWDGDDQAAGFVASDVGWVGLKFLETPNDPATGEPRGVTT
                     LDIFQYSAAPNSDVTGYDQMAGALHVGLPSTDPQQGGGTPLQRPDWPDGAPGDAFQIQ
                     NSYGPDITITAASGPYTMAPGESLPFTFASVHGTSRSDIVNNAKLTQILYNAQYRAAS
                     GPPIPKVVAVPGDGSVTLYWDDAAELGYYPDGTYGDPLTGNNAFEGYKIFRSDDGGET
                     WGKPIVDLFGAVQGYIPLAVFDLNNRFSGESDTRRHFNLGTNSGLRHTWTDSNVNNGQ
                     EYLYAVVAFDSQDGPVPPLETPINAANPDAPDDNTVRVVPMARPAGEAAGRVFNAVDQ
                     RDVAIHVAGSSDVAEIPVTLVDPNALVAGTYEVSFDYDEGELVYRVERGGEVVDDMSG
                     RPVEGIPLFDADTDFAPIFAGMRLTVENVPFGLKNDYQVEGTGLEAQSSYYMGWDFGE
                     GMPSNQVMDYELRFVASDLTYTEYYDGVAVTAPFQAWNVTTGEQITVEIIDSDGSGTW
                     NGTEWIIFVNTGYTGSGEWEGAYPDDYALYVRFTSGSTYAAGDIYRFETNKALTDADR
                     YEFLVAAPVYSEELLADDLELITVVPNPFVVSSQYERGRFGVQRILQFHQLPEECTIR
                     IYTTAGELVQKLEHDGGSIEPWNLQSYNGQEVGFGVYLYMVQTPDGAEHTGKFAVIK"
     gene            6077..7219
                     /locus_tag="AC16YU_12195"
     CDS             6077..7219
                     /locus_tag="AC16YU_12195"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR6066032.1"
                     /translation="MKSIRLILAALVLCAVSGPASGQGFNNAGTSVANFLKIPVGARG
                     TALGGAYSALSSDATSLYWNVAGIMGVENNEVAFVHNEWLLDLSHEYIAGVFRLGTND
                     RIGLSISYLDIGEMEETSPAQPQGTGLFFSAYDVAIGVAYARQLTDRFALGINTKYVQ
                     ESIAKSSASGVAFDLGLLFRAEWNDLRLGATISNFGPDLRMDGENLRTKLDPYPTAGD
                     SPDDVPLFLETETFALPIQFQFGAAITPYTTESLALTTVLDIRDTRDFNQEIRLGAEV
                     EVMNAFFLRGGTNLFLVEGSPFGNGDDTVIEGSDTADPTGIGPSYINPTTITRSDLRD
                     GQALYNLGLGLDVVLPSTGMNFRFDYAFSRIRTLDDVHRFGVALAF"
     gene            complement(7239..8531)
                     /locus_tag="AC16YU_12200"
     CDS             complement(7239..8531)
                     /locus_tag="AC16YU_12200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6066033.1"
                     /translation="MIHRSVAPVFLATLLAAMAGPLSSSAQPSGLMAIRAGSMLDVKA
                     GVLVPDAVVLVEGNRIAAAGSGLPVPAGARVVDLGDAVLLPGLIDAHTHLCIQPDYAA
                     NNPILYKSIPYQTAEAVAAARATLMAGFTTIRDVDSEGADWADVAVRDAIEDGVVPGP
                     RMQVSTRALSITGGYMNNDGLAPQIDVPQFGALVDSPEAIVEEIRLEVKHGTDWIKLY
                     ATGTTRHINLDDMTPLPQFTREEIALVMREAERFRVPVAAHAYGGQGALDAVTLGVRS
                     IDHGMLLDDRILDEMAARGTFWVPTISVYFGEGPRESWDERTVAIVDAHRDTFGRALG
                     RGVRIAYGTDAGALPHGTNAVDFPVMVEYGMAPIDAIRSATMNAAELMRMDGLVGSIE
                     AGKLADLIAVPSSPLDDVRVLSDVLFVMKDGEVYRNER"
     gene            8699..9097
                     /locus_tag="AC16YU_12205"
     CDS             8699..9097
                     /locus_tag="AC16YU_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066034.1"
                     /translation="MLRALKVVALMAVVPLAAIACGDNVVGPENEPEVTNETDTFQWQ
                     VSDLESVSQTFTYDWQNTGLTANVDQSSAVTGGTAVLKIRGTDGTLLYSRSLLEDGTF
                     ETDSGDPGEWTIEVTLSDVTGTLNFRVQKP"
     gene            9247..12258
                     /locus_tag="AC16YU_12210"
     CDS             9247..12258
                     /locus_tag="AC16YU_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR6066035.1"
                     /translation="MSRVSLVLALALLAFVHDTRAQSLGDAARTLDFRMVGPTRGGRV
                     TAVAGHPATPGTYYMGATGGGVWKTTDYGQSWRVVSDGAFESPSIGSIDVADSDPDIV
                     YVGTGSDGIRSNVIIGRGIYRSNDAGETWSFLGLRDTGQIGAVVVHPTNPEVVWVAAL
                     GSPFGPGPERGVYRSVDGGATWKHVLFVSDRTGAVDLELNPANPDEVYAALWHAERKP
                     WTILSGDDSESGIYRSTDAGATWRKVEAGLPEGLIGKIDLAVTPADPSRVYALVEAAP
                     DVEGLYRSNDRGETWLHVSAEGGLMRRPFYYTNVDADPVDPDVVYVGNESFFRSGDGG
                     TTWERRSTPHGDNHDLWIDPHDTDVWIQSNDGGANVTRDGGRTWSTQHNQPTAELYQV
                     HVDDRFPYWLYAGQQDNSTIAVPSLPTGDREAGQTAYWESTGGCETGPVVPKPGDPDV
                     VYANCKGRFGVYDRRTGQERQYAVGAVDLYGVNPSELPFRFQRVVPVEVSPHDPDVVY
                     HGSQYVHRTADGGRTWERISPDLTANRTERQVVSGSPITRDITGEEHYSVLYVIEESP
                     VAPGVIWAGSNDGPVHVTRDGGATWEDATPPMPPEGRVNGIDPSPHRAGTAYVAAYRY
                     LLDDFAPYAFRTDDFGETWTRISDGFPADSPVRVVREDPVRPGLLYAGTETGLFVSLD
                     DGASWTAFQGDLPVTPITDLRVHRGDLVLSTMGRSFWILDDVSWLREWSGEIGSADAH
                     LFGPRDAYRMRYSSSRGGDHGPEYPAPGATIRYHLGRDVESAALEILDASGAVVRSLE
                     AGAAEDGAEDPDQGMRGPRSAAAPGGALGTTTGLHRFTWDLRHEGASAPGGRPSRGPM
                     AVPGSYRVRLTAGGVTLDRPFDVLVDPRVAAAGVTQADLEAQLAHNLEVRDLVSAFRD
                     GLARVDTLLARAEGDVKAAYAALRAEVVTDNADSYPPRLLDSQLSYLASMTTVADQAP
                     GGDAVERLGQLRAQVEAWLERVAATMEEGRP"
     gene            12364..12807
                     /locus_tag="AC16YU_12215"
     CDS             12364..12807
                     /locus_tag="AC16YU_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR6066036.1"
                     /translation="MLSKTSQHAIRAMVAIAQRREGGFVTAKAVSEALGLPPSFLAKV
                     LQRLTSAGLLDSLRGPTGGVALARPAESIRLVDIVEAIDGRAPFGSCLFGWPGCSSDR
                     PCPLHREWGKALQPLLDLLETATLADTTELVTIGTAEDGLLHEAI"
     gene            12839..13300
                     /locus_tag="AC16YU_12220"
     CDS             12839..13300
                     /locus_tag="AC16YU_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6066037.1"
                     /translation="MRHTISASLVLTVLLLAAGCGGGSPEPQASSTPSAGSDLSAAEL
                     EHGIGPISAFTLDAVDPALAATGEEVFTVKCSACHKMSERYVGPALGDVTTRRSPAYI
                     MNMILNPEGMVQRHPEARKLLAEFMTPMANQNLTEEEARAVLEFLRTQAPQ"
     gene            13327..15020
                     /gene="nosZ"
                     /locus_tag="AC16YU_12225"
                     /pseudo
     CDS             13327..15020
                     /gene="nosZ"
                     /locus_tag="AC16YU_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009745144.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
BASE COUNT         2611 a         5151 c         5039 g         2219 t
ORIGIN      
        1 ggtgctcggt gagcggctca tccgccagga cgaggtctcg acgacgacgg tcatgcgcgg
       61 ggaggagatc caggcgttgc cggtggacaa cttccaggac gcgatctccc tcgccggcgg
      121 cgtggtcgag aatgcggccg gccaggatcc gggcatccac gtgcgcggcg gccggacgtc
      181 cgagatcgcg tacctcgtcg acggagtgct cgtgcaggat ccgctgtccg gcagcctggg
      241 cggcttcgac gtggcccgcc agtccatcga cgagctccag gtgctcaccg gcggattcag
      301 cgccaagtac ggcaaggcga tgtcggggat catcctcgtg aacacgcccg cgggcggcaa
      361 cgagtaccac ggagcccttc ggctgcagac cgacggcctt tcgatggagg gcgtcggcca
      421 gggcggcccc gcgctcgact accgccggcc gttctcgaac gactggggga cccggagcgt
      481 cgagggaacg atctccggac ccatcgtcaa ggaccgtgtg acgttcttcc tgaacgcgga
      541 ccgcctggat tccgactcgt acctgagcga gttcgagggg ccggtccgcc cggcgatgct
      601 gccgtggagc caactggagg gcctgacctt cctgcgcccc ggcaccacca gcctcatcga
      661 cgcgaacggc gccccggtcg aactcgatct cgagaccctc ggcatcgagg agggaacggt
      721 cgtcacggcc gagatgatcg aggatctgcg cgaggcgggc gtggacgtgg accggcaggc
      781 gaaggccgcc gtgagcgact accggcagga tcaccagttg ggcctctacg acgaccgcac
      841 gcggttcgtc gccaacctgg gcatcaaggt caacgacgac atagacacca ggctcgggta
      901 caagctgacc cgccgcgagt ggcgcaccta cgaccacgga ttcaagtacc tgccccagta
      961 caacgacaag tcacagaggg agatcgacgt gttcagcgcg caggtcacgc atcgcgtggc
     1021 gtcgaacatg ttctacgagc tccgggcatc gtatacgggc aaccggtact acgaattcct
     1081 gtacgacgag tcgaaggacg agaacgatcc ctggcggttc ggccgcctgt tcagcccgta
     1141 cagcgtggac ggtgccttca acacggaagg cctcgcggac gcggaggggg gcaacaacta
     1201 cgacttcctg ggctactcgg aggtcgggat ctacgtcgac tacccgtacc tcctgaccgg
     1261 cgacctggac cagcccttcc agctgtcccg gaacctggac ctggacgggg acggcaccgt
     1321 ggaccatttc gagggcgacc gcctcacgga agacctggta caggagctca aggacctgta
     1381 cgcgaccctg tcggacgagg agatcgaggc gatgggcctg agcaagaacc tgatccccgt
     1441 cctcgcgccg ccgacggagc gggactacga ggagcggaac atcaaggact tccagctcgg
     1501 actggacttc acggcgcagg tcgacgaccg gaacatgctc caggcggggg tggagttccg
     1561 gaagatgagc ctgttccggt acctgatctt agcgaacacg tactgggatc cgcagaccga
     1621 tccgtccctg gagacgacgc tcacgcaccg gccagaggac gagttctacg tggacgtgaa
     1681 accgttcgag gttgcggcct acgtcgagga caagatcgag ttcggcagtc tggtgatgcg
     1741 cccgggcctg cgcctggacg tgctcgatcc ggacatggcc acgctcgcag actacggcca
     1801 ggtgaccgtc atgcagcggc cgagccagcc gaacgatccg aggcccgaaa ccgtgcaggc
     1861 ctcggtcaag tggcggatca gcccgcgctt cggcatcgcg ttcccgctca ccgaccgggc
     1921 gaggctctcc ttcaactacg gccagttcct ccagtatccg gagtacaacc ggctctacga
     1981 aagctatcgc acggccgcct ggataccggg ggacgggtac gtccctctcg aagagttcgg
     2041 cttcgacctg ggcttcgagt ccttcatcgg caacccggac atcaagcccg agaagaccat
     2101 cttctaccag gtggacgggg agttcctcgt gagcgagtcg ttcaagctcg gcgcgtcgct
     2161 gttctacaag gacatctacg actacgtggc ggtgagccgc acgctgaccc agggcggcca
     2221 gttcgtctgg gtgatggaca acctcgacta tgcgaactcg cgcggattcg aggttcgcgc
     2281 ggagaagcgg ctcgaggaat acctcggctt ctcggcgagc tatacgtaca gccggtcggt
     2341 cggcaacgcc gacgactacg ccgggcgata cgacgactgg tacaacaact ccgtcgccgg
     2401 gacgattccg gccaagaagg ccgagccgct ggactgggac cagccgcaca cgctgggctt
     2461 catggtgaac ggcaagtaca gcggactcaa ggggagcctg ctgggacggt tcgggagcgg
     2521 cctcccgtac acgccgacga ccacgcgcgg cctgccgctc gggcccaaga actcggcgcg
     2581 aaggccgtgg accggctcga tcgacgcccg gctcgaatac cgcttcgacc tgcctcggta
     2641 cggatggatc acgcccttct tcgaggtgac caacctcctc gaccggaaga acgtgctcgc
     2701 cgtgtactcg gacacgggat ccccggactt cacgctcgat ccgggcaccc agtacgagga
     2761 cgcgcagcgt ccgaacctca tcgggccgcc gcgccacttt gaagtgggct tcacgctcgg
     2821 attctgatcg cgggcggacc ctgccgggcc gggcacgcgc ccggtccggc gccgctcccc
     2881 aaacgctcct gcttcagacc acaggctatc agctatgaag cgaccagtga acatacaggg
     2941 ccgcccgctg gccatcctca ccggattgct ggtgacgtgg gccggcctgg cgttttcggc
     3001 ccgtgcccaa cagggcatga cgaaggcgca gtacatggac tatctcacgc ggtcgggccg
     3061 catcgagtac ccggccggcc cgatcatcgc ggggaagacc ctggggccgg ccgacctcaa
     3121 gctcagcgtg ctcgacatcg gcaatgtccg ggcccgcatc cggaacacgg gcacgctcgg
     3181 gtacgaccgc gacctgctgt gctacgagtt cccgttcaac agcggaatca cgtaccgatg
     3241 gacgctcggc cccatgatcg cgggccggat cgacggtcag aagtacgtct ccggcgcggc
     3301 gctcggcgcc gtgcgcggca ccacggagga cgagttcagg ccgctctacg gcttcgactc
     3361 gggcgactac cggtccgagc tcaacatcgg catcgcgttc tccgacaagc cggcgtcctg
     3421 gccctcgtcg tggccgacga tcgacgaggc cctggcggac aacgggcagc acatcctgaa
     3481 tccggccgcc cggaccgcct tcctcgccaa ccccacgttc gacccggtgg cgacagggac
     3541 ggcacggatc ggctcgaagg gattcccggg cgtcgccgac ggcgagatcc gcgcgccgcg
     3601 cgaggcctac ttcgtgatca cggacaacga ccctgcccag ccggcggcgc cgctgcccat
     3661 gaacgtgcgg gtggacgtgt gggcgatgca gtgggacgac ttcatcaacc gcaacttcat
     3721 catctacaag atggtcttca ccaacatcgg tgaaaagacc atcgagaacg tgtacatcgg
     3781 catccacgac gacccggacg ctcccgagca ggggtcgaac gagtggacgg acgactacgc
     3841 gtacctggtg ccgccgggca aggacaccga cggcgacggc gttccggatc ccgatgccga
     3901 cccgcgcgcg gacgtcgacg gggacggcat gtacacgggc gaggactcgc tgctctggaa
     3961 caccgtctac ctgtgggacg gcgacgacca ggcggccgga ttcgtggcga gcgacgtggg
     4021 atgggtgggc ctcaagttcc tcgagacgcc gaacgacccg gcgacaggcg agccccgcgg
     4081 cgtcacgacg ctcgacatct tccagtactc cgccgccccg aactccgacg tgacggggta
     4141 cgaccagatg gcgggcgccc tgcacgtggg cctcccgagc acggatccgc agcagggagg
     4201 cggcacgccg ctccagcggc ccgactggcc ggacggagcc ccgggcgacg ccttccagat
     4261 ccagaactcg tacggtccgg acatcacgat caccgcggcg tccggtccct acaccatggc
     4321 gccgggcgaa agcctgccgt tcactttcgc gtccgttcac ggcacgagcc gatcggacat
     4381 cgtcaacaac gcgaagctta cgcagatcct gtacaacgcc cagtacaggg cggcctccgg
     4441 tcctcccata ccgaaggtcg tcgcggttcc cggagacggg tcggtgaccc tgtactggga
     4501 cgacgcggcg gagctcggct actatcccga cgggacgtac ggggacccgc tgacgggcaa
     4561 caatgccttc gaggggtaca agatcttccg ctcggacgac ggcggggaga cctggggcaa
     4621 gccgatcgtg gacctcttcg gcgccgtgca ggggtacatc ccgctggcgg tcttcgacct
     4681 gaacaaccgc ttctcggggg agagcgacac gcgccggcac ttcaacctgg gcaccaactc
     4741 gggactgcgc cacacctgga cggacagcaa cgtcaacaac ggccaggagt acctgtacgc
     4801 ggtcgtggcc ttcgattcgc aggacggccc ggtgccgccg ctcgagacgc cgatcaacgc
     4861 ggcgaatccc gacgcgccgg acgacaacac ggtccgcgtg gtgccgatgg ctcgcccggc
     4921 gggtgaggcc gccggccggg tgttcaacgc ggtcgaccag cgggacgtgg ccatccacgt
     4981 ggccggcagc tccgacgtgg ccgagattcc ggtgacgctc gtcgatccga acgcgctcgt
     5041 cgcgggcacc tacgaggtgt ccttcgacta cgacgagggc gagctggtgt accgggtgga
     5101 gcgcggcggg gaggtggtcg acgacatgtc gggccgtccg gtcgagggca tcccgctctt
     5161 cgacgcggac accgacttcg cgcccatctt cgccggcatg cgcctcacgg tcgagaacgt
     5221 tccgttcggg ctcaagaatg actaccaggt agagggtacc ggcctcgagg cccagtcttc
     5281 ctactacatg gggtgggact tcggcgaggg gatgccctcg aaccaggtca tggactacga
     5341 gctacgcttc gtggcgagcg acctgaccta caccgagtac tacgacgggg tcgcggtcac
     5401 ggcgcccttc caggcctgga acgtgaccac cggggagcag atcacggtcg agatcatcga
     5461 ctcggacggc agcggcacat ggaacggcac ggagtggatc atcttcgtga acaccggcta
     5521 caccgggtcc ggtgaatggg aaggggccta cccggacgac tacgccctgt acgtccgctt
     5581 cacgagcggc tcgacgtacg cggccggcga catctaccgg ttcgagacga acaaggcgct
     5641 gacggatgcc gaccgctacg agttcctcgt ggccgcgccg gtatactcgg aagagctgct
     5701 cgcggacgac ctggagctca tcacggtcgt gcccaatcca ttcgtggtat cgagccagta
     5761 cgagcgcggc cggttcggcg tgcagcgcat cctgcagttc catcagctgc ccgaggagtg
     5821 cacgatccgg atctacacga cggccggcga gctcgtccag aagctcgagc acgacggcgg
     5881 ctcgatcgag ccctggaacc tgcagagcta caacggccag gaggtgggct tcggcgtgta
     5941 cctgtacatg gtgcagacgc ccgacggagc cgagcacacc ggcaagttcg cggtgatcaa
     6001 atagaacatg ctgacggcga acgcggggcg gccccgggcc gcctcgccgc accccattca
     6061 aggacgagaa agcatcatga aaagcatacg actcatcctg gcggccctgg tgctctgcgc
     6121 ggtcagcggc cccgcctccg ggcagggctt caacaacgcc ggaacgtctg tggccaactt
     6181 cctgaagatc ccggtcgggg cccgcggaac ggccctgggc ggcgcctaca gcgccctgtc
     6241 gagcgacgcg accagcctgt actggaacgt cgccggcatc atgggcgtgg agaacaacga
     6301 ggtggccttc gtgcacaacg agtggctgct cgatctctcg cacgagtaca tcgccggcgt
     6361 cttccgcctc gggaccaacg accggatcgg cctctcgatc tcgtacctgg acatcggtga
     6421 gatggaggag acctcgccgg cgcagcccca gggcacgggc ctgttcttca gcgcgtacga
     6481 cgtggccatc ggcgtcgcgt acgcccggca gctgacggac cggttcgcgc tcggcataaa
     6541 cacgaagtac gtacaggagt cgatcgccaa gtcgtcggcc tccggcgtcg ccttcgacct
     6601 gggcctcctg ttccgggcgg aatggaacga cctgcgcctc ggcgccacga tctcgaactt
     6661 cggcccggac ctgcggatgg acggcgagaa cctccggacc aagctcgatc cgtatccgac
     6721 ggcgggcgac agcccggacg acgtcccgct gttcctcgag accgagacct tcgcgctgcc
     6781 gatccagttc cagttcggcg cggcgatcac cccgtacacg acggaatcgc tggcgctgac
     6841 gaccgtcctg gacatccggg acacgcgtga cttcaaccag gagatccgcc tcggcgccga
     6901 ggtcgaggtg atgaacgcgt tcttcctgcg cggcggaacg aacctgttcc tggtcgaggg
     6961 cagccccttc ggcaacgggg acgacacggt gatcgaggga tcggacacgg ccgacccgac
     7021 gggcatcggc ccttcgtaca tcaacccgac cacgatcacc cggagcgatc tccgcgacgg
     7081 gcaggccctg tacaacctgg gcctcggcct ggacgtggtt ctgcccagca cgggcatgaa
     7141 cttccgcttc gactacgcct tctcccggat ccgcacgctc gacgacgtgc accgcttcgg
     7201 ggtggcgctg gcgttctgaa ccgggtccga ccgcggccct agcgctcgtt ccggtacacc
     7261 tccccgtcct tcatcacgaa gaggacgtcg gagagcacgc ggacgtcatc gagcggactg
     7321 gacggcacgg cgatgaggtc cgcgagcttc ccggcctcga tcgagccgac gaggccgtcc
     7381 atccgcatga gttccgcggc gttcatcgtc gccgaccgga tcgcgtcgat cggcgccatt
     7441 ccgtattcga ccatcacagg gaagtccacg gcgttcgtgc cgtggggcaa cgcgccggcg
     7501 tccgtgccgt aggcgatgcg gacgccccgg ccgagcgccc gcccgaacgt gtcgcggtgc
     7561 gcgtcgacga tcgcgacggt gcgctcgtcc cacgattcgc gcggcccctc gccgaaatag
     7621 accgaaatcg tcgggaccca gaaggtgccc ctcgccgcca tctcgtccag gatccggtcg
     7681 tcgagaagca tcccgtggtc gatggaccgg acgccgagcg tcacggcgtc gagcgcgccc
     7741 tgcccgccgt aggcatgggc ggcgaccggc acgcggaacc gctccgcctc gcgcatcacg
     7801 agcgcgatct cctcgcgggt gaactgcggc aggggcgtca tgtcgtccag gttgatgtgc
     7861 cgcgtggtcc cggtcgcgta cagcttgatc cagtcggtcc cgtgcttcac ctcgagacgg
     7921 atctcctcga cgatggcctc cggcgagtcg accagcgccc cgaactgggg cacgtcgatc
     7981 tgcggcgcca gcccgtcgtt gttcatgtac ccccccgtga tcgagagtgc gcgcgtggac
     8041 acctgcatgc gcggtcccgg gacgacgccg tcctcgatcg cgtcccgcac ggccacgtcg
     8101 gcccagtccg cgccctcgga gtccacgtcg cggatcgtgg tgaaaccggc catgagcgtc
     8161 gcgcgtgccg cggccacggc ctcggccgtc tggtagggga tcgacttgta caggatgggg
     8221 ttgttcgcgg cgtagtccgg ctggatgcac aggtgcgtgt gcgcgtcgat gagccccgga
     8281 agcagcaccg cgtccccgag gtcgacgacc cgggcccccg caggcacggg gagacccgac
     8341 ccggcggccg cgatccggtt gccctcgacc aggacgaccg cgtccggaac gaggacaccg
     8401 gccttgacgt ccagcatcga gccggcccgg atggccatga gccccgaagg ctgggcggag
     8461 gacgacaggg ggcctgccat ggcggccagg agggtcgcga ggaataccgg ggcaacggaa
     8521 cggtggatca tggcgggcgc gggatgaggc attctcccca cgatacgcaa gtcggtgctc
     8581 gcaagctgac gccgtggtgt gcgggggacc cgccgggatc cttttcagaa ggcctcccgt
     8641 tatgccgata cccggaggcc acccgggacg gtttcaccac gaacgcagcg agggagccat
     8701 gcttcgtgcg ctaaaggtcg tcgctctcat ggccgtagtt ccccttgcgg ccatcgcctg
     8761 cggcgacaac gttgtaggac ctgagaacga gcccgaggtc acgaacgaga ccgacacctt
     8821 ccagtggcag gtttccgatc tcgagagcgt ctcgcagacg ttcacgtatg actggcagaa
     8881 cacgggtctc acggccaacg tggaccagtc gtcagcggtc acgggcggaa cagcggtact
     8941 gaagatccgc ggaacggacg gaacactcct ctactctcgc agcctgctcg aagatggcac
     9001 gttcgagacg gattcgggcg atccgggcga gtggaccatc gaggtgacgc tgtccgacgt
     9061 gaccggcaca ctgaactttc gcgtgcagaa gccctgatcc ccgactcgcc ggggtgcccg
     9121 gcaggccgag gcgggatcgg agtgcggacg acaggcccca cgggcgatct ccggacagtc
     9181 gaaccggtcg atcctgacac cactgggccc gatattgggc gcgccagccc aatatcccgc
     9241 ccatcgatgt cccgagtatc gctcgtcctc gctctcgccc tgctcgcttt cgtccatgac
     9301 acgcgggccc agtccctggg ggacgcggcc cgcacgctcg acttccgcat ggtgggcccg
     9361 acccgcggcg gccgcgtcac cgccgtggcg ggccaccctg cgacgcccgg cacgtattac
     9421 atgggcgcga ccgggggcgg cgtctggaag accaccgact acggacagtc gtggcgggtc
     9481 gtctctgacg gtgcgttcga gtccccgtcg atcggatcca tcgacgtggc cgactccgat
     9541 ccggacatcg tgtacgtggg cacgggctcg gacgggatcc gctcgaacgt catcatcggc
     9601 cgcggcatct accgctcgaa cgacgccggc gagacgtgga gcttcctcgg gctgcgggac
     9661 acgggccaga tcggcgccgt ggtcgtccac ccgacgaacc ccgaggtggt ctgggtcgcg
     9721 gcgctcggca gcccgttcgg gccggggccg gagcgcggcg tgtaccggtc ggtcgacggc
     9781 ggcgccacgt ggaaacacgt cctcttcgta tccgaccgga cgggcgccgt cgacctggaa
     9841 ctcaaccccg cgaacccgga cgaggtgtac gcggccctct ggcacgccga gcgcaagccc
     9901 tggaccatcc tgagcggcga cgactcggag agcggcatct acaggtcgac cgacgccggg
     9961 gccacctggc ggaaggtcga ggccggcctg cccgaaggcc tcatcgggaa gatcgacctc
    10021 gcggttacgc cggccgaccc gagccgcgtc tatgcgctgg tcgaggcggc gcccgacgtc
    10081 gagggtcttt accggtcgaa cgaccggggc gagacctggc tgcacgtttc cgcggagggc
    10141 ggcctcatgc gccggccctt ctactacacg aacgtcgatg cggaccccgt cgacccggac
    10201 gtggtctacg tgggcaacga gagcttcttc cggtcagggg acggagggac gacgtgggaa
    10261 cgccgctcca cgccccacgg cgacaaccac gacctgtgga tcgacccgca cgacaccgac
    10321 gtctggatcc agtcgaacga cggcggtgcc aacgtgacgc gcgacggcgg ccgcacgtgg
    10381 tccacccagc acaaccagcc gacggccgag ctctaccagg ttcacgtcga cgaccgcttt
    10441 ccgtactggc tgtacgccgg ccagcaggac aactccacga tcgccgtgcc gtcgctaccg
    10501 acgggggacc gcgaggcggg ccagaccgcg tactgggagt cgaccggcgg ctgcgagacc
    10561 gggcccgtcg tgccgaagcc cggcgacccc gacgtggtct acgccaactg caagggccga
    10621 ttcggcgtgt acgaccgccg aacggggcag gagaggcagt acgcggtcgg cgcggtcgac
    10681 ctctacggcg tgaacccgtc cgagctgccc ttccgtttcc agcgcgtcgt gccggtcgag
    10741 gtgtcgccgc acgatcccga cgtcgtgtac cacggatcgc agtacgtgca ccgcacggcc
    10801 gacggcggcc gcacgtggga acgcatcagc ccggacctga ccgcgaaccg gaccgagcgg
    10861 caggtcgtct ccgggagccc gatcacgcgc gacatcacgg gggaggagca ctactccgta
    10921 ctgtacgtca tcgaggagtc gcccgtcgcg ccgggcgtga tctgggccgg ctcgaacgac
    10981 ggccccgtgc acgtgacgcg cgacggcggc gcgacctggg aggatgcgac gccgcccatg
    11041 ccgcccgagg gccgcgtgaa cggcatcgat ccgtccccgc accgggcggg cacggcctac
    11101 gtggcggcct accgctacct gcttgacgat ttcgcgccgt acgccttccg gaccgacgac
    11161 ttcggagaga cctggacgcg catctcggac ggctttccgg ccgactcgcc ggtccgcgtg
    11221 gtgcgcgagg acccggtgcg tcccggcctg ctgtacgcgg ggacggaaac ggggctcttc
    11281 gtctcgcttg acgacggggc gtcctggacg gcgttccagg gcgacctccc cgtgaccccg
    11341 atcacggacc tgcgcgtgca ccgcggcgac ctggtcctct cgacgatggg ccggtcgttc
    11401 tggatcctgg acgacgtgtc ctggctgcgc gagtggtcgg gcgaaatcgg gtcggccgat
    11461 gcgcaccttt tcggcccccg ggacgcgtat cggatgcggt attcgtcgtc gcgcggcgga
    11521 gaccatggcc cggaataccc ggcgccggga gccacgatcc ggtaccacct gggcagggac
    11581 gtggaatccg ccgctctcga gatactcgat gcctcgggag cggtcgtccg ctccctcgaa
    11641 gccggagcgg cggaggatgg cgccgaggat ccggaccagg gaatgcgtgg cccccggtcg
    11701 gccgcggcac cgggcggagc gctcgggacg acgacgggcc ttcaccgttt cacgtgggat
    11761 ctcaggcacg agggcgcctc tgcaccgggc ggccgcccat cgagggggcc catggcggtc
    11821 ccgggatcct accgggtgcg gctcaccgcg ggcggagtga cgctcgaccg accgttcgac
    11881 gtgctcgtcg atccccgcgt cgcggccgcc ggcgtgacgc aggccgatct cgaggcgcag
    11941 ctcgcgcaca acctcgaggt ccgcgacctg gtctcggcct tccgggacgg gctcgcacgc
    12001 gtcgacaccc tcctggcccg ggccgagggc gacgtgaagg cggcgtacgc ggcccttcga
    12061 gcggaggtcg tgaccgacaa cgccgactcg tacccgccgc ggctcctgga ctcgcagcta
    12121 tcctacctgg cgtcgatgac cacggtcgcc gaccaggccc ccggcggcga tgccgtggaa
    12181 cggctcgggc agctgcgcgc gcaggtggag gcgtggctcg aacgggtcgc tgcgacgatg
    12241 gaggaggggc gcccgtaggc cgaccgtggc gacgtcttgt gaatgcagcg tggagaacgc
    12301 cctcccggct tgcgctcgcc ccgcgcagat cgtaaataca gataagggac tcgtaaatgc
    12361 gaaatgcttt ccaagaccag ccagcacgct atccgggcca tggtggccat cgcgcagcgg
    12421 cgtgaagggg gattcgtgac ggcgaaggcc gtgagcgagg cgctcgggct gccgccgtcc
    12481 ttcctcgcga aggtcctgca gcggctcacc tccgccggac tcctggattc gcttcgcggt
    12541 ccgaccgggg gcgtcgcgct ggcgaggccg gcggagtcca tccgcctggt ggacatcgtc
    12601 gaggccatcg acggccgggc ccccttcgga tcctgcctgt tcgggtggcc cggatgctcg
    12661 agcgatcgcc cctgcccgct ccaccgggag tgggggaagg cgctgcagcc gctgctcgac
    12721 ctgctggaaa cggccacgct ggccgacacc accgaactcg taacaattgg aacggccgaa
    12781 gacggccttc tccatgaagc catataggac aacagactcg tcaacccgaa tcagatccat
    12841 gagacacacc atttctgcat cgctcgtcct caccgtcctg ctcctcgccg cggggtgcgg
    12901 gggcggatcg cccgagccgc aggcctcgtc gaccccgtcg gccggaagcg acctcagcgc
    12961 cgccgagctc gaacacggca tcgggccgat ctcggccttc acgctcgacg ccgttgaccc
    13021 ggccctcgcc gcgaccggcg aggaggtgtt cacggtgaag tgctcggcct gccacaagat
    13081 gagcgagcgc tacgtgggcc cggcactcgg tgacgtgacc acgcgccggt cgccggccta
    13141 catcatgaac atgatcctga accccgaggg aatggttcag cgccacccgg aggcccgcaa
    13201 gctcctggcc gaattcatga ccccgatggc caaccagaac ctgaccgagg aagaagcccg
    13261 ggccgtgctc gagttcctcc ggacccaggc cccccagtaa gccccctaaa ccaaccctgg
    13321 actgaaatga cacgctcgaa cctcaaacac ctgctcctcg gggcggccgc ggtcgcactc
    13381 gtggcgacga tcgcgacgtc ctgcagcaac ccggagggac tgggtgccgg ctccggcaac
    13441 gccgcctcca aggtctacgt cgcgccggga tcgtacgacg agtactacgc cttcatgtcg
    13501 ggcggctact cgggccagat caccgtccac ggcctgccct cgggacgcct gctcaagacc
    13561 gtggccgtct tctcgcagtt ccccgagaac ggctggggct acgccgagga gacgaaggcg
    13621 atgctgaaca cgtcgtacgg cttcattccg tgggacgact cgcaccaccc gcagctctcg
    13681 cagaccgacg gcgtgcccga cggccgctgg atcttcatca acgcgaacaa cacgccgcgc
    13741 gtggcccgga tcgacctggc gaccttcgag accgaggaga tcctcgagat cccgaactcc
    13801 gccggcaacc acgcctcgcc gttcatcacc cagaactcgg aatacatcgt cgcggcgacg
    13861 cgcttctcgg tgccgattcc gcagaaggac atctcgatcg cggagtacaa ggagtagttc
    13921 aagggcaaca tctcgttcgt gcgggccgac cagccgggcc agatggacgt cgcattccag
    13981 atcatgatgc ccggctacga ctacgacgtg tcgcacgcgg gcaagggccc ctcgcacggc
    14041 tgggcgttct tctcgaccta caacaccgag caggcgaaca cgctcaagga ggtcaacgcg
    14101 tcgcagaacg acaaggactt catcgcggcc gtcaactgga agcggggcga ggagtgcgtc
    14161 gcttcgggcg ccgcgaagca gttcggcggc gtgtacgtcc acaaccacat gggcgctgac
    14221 cgcgtcgccg tctcggagac ccgcgagggc gtgaaggtcc tgtacccgga ggactgcgag
    14281 gggctcgtgt acttcctgcc cacgcccaag tcgccgcacg gcgtggacgt ggatccgacg
    14341 ggtgagtaca tcgtgggcgg tggcaagctg gcgaccgtga tcccggtcca ctccttctcg
    14401 aagatgctcg cggcgatcga ggccggggcg ttcgagggcg aggcggacgg catccccgtc
    14461 atccagtacg aggccgtgat cgccggcgag gtcgagaacg gaggcctcgg gccgctccac
    14521 accgagttcg acggcaaggg cttcgcgtac accacggcct tcctgtcgtc cgaaatcgtc
    14581 aagtggcaga tcgggacctg gcaggtcgtg gaccgcatcc cggtctacta ctcggtgggc
    14641 cacctgatga taccgggcgg ggacacgcgc cagccgtggg gcgagtacct ggtcgcgctc
    14701 aacaagatca ccaaggaccg ctacctcccg acgggtcccg agctcgccca cgcagcccag
    14761 ctgatcgaca tttcgggcga gaagatggaa ctgctcctcg acttcccgac gatgggcgag
    14821 ccgcactacg cgcagggcat cgcggcggag ctggtcacgc agcgccaggt gcagctcttc
    14881 aacatcatgg agaacacgaa cccgaacgcc gtgagatccg aagcggaagc cagggtcgag
    14941 cgcaacggca acgaagtgca cgtctacgca tccgcgatcc gctcgcactt cgcgccggac
    15001 aacatcgagg gcgtgaaggt
//
LOCUS       DBNHQZ010000450        16633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2031060, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000450 DBNHQZ010000000
VERSION     DBNHQZ010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 16633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16633
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2031060"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="AC16YU_12230"
     CDS             complement(<1..422)
                     /locus_tag="AC16YU_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6066038.1"
                     /translation="MPSLVVPGRAEPVAIGKIIGIGQNYARHAAEMRSAPPEEPMLFL
                     KPSTAVVRDGGEVVLPGVSGEVHHEVELVVVVGRGGRWIPESEALSHVAGYAVGLDMT
                     ARDLQARAKEKGHPWAVAKGFDSFAPLGPVVAAGSVPDP"
     gene            544..855
                     /gene="rplU"
                     /locus_tag="AC16YU_12235"
     CDS             544..855
                     /gene="rplU"
                     /locus_tag="AC16YU_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014940015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6066039.1"
                     /translation="MFAIIEIGGKQYKVRENDRIYVPRQDAEPEATLTFDRVLLVSND
                     DAVQVGTPVVDGASVDATVLAQVKADKVLIFRKKRRKRFRVKRGHRQPYTQIRIDAVK
                     A"
     gene            893..1153
                     /gene="rpmA"
                     /locus_tag="AC16YU_12240"
     CDS             893..1153
                     /gene="rpmA"
                     /locus_tag="AC16YU_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6066040.1"
                     /translation="MAHKKGMGSTKNGRDSQPKMLGVKAFAGEHVQAGAIIVRQRGTK
                     FHPGTNVGRGNDDTLFATATGTVRFARGRNDRRFVHVDPAQA"
     gene            complement(1229..2296)
                     /locus_tag="AC16YU_12245"
     CDS             complement(1229..2296)
                     /locus_tag="AC16YU_12245"
                     /inference="COORDINATES: protein motif:HMM:NF045046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6066041.1"
                     /translation="MKRILVTGAGSNAGRNVVAALRMADETAFVVGADTNAFHLACAD
                     VDASYLLPQPSEPGFLEALHGIVSDERVTYVHPQPDAELAFLSEHRDELPGLLLIPDH
                     ADLVAARNRLHTNRLLTAADVPVPTVVRLHQNIDTAQALAEVAERSGRACVRTIQSRT
                     GIGSLPVGTGEQISAWLTYWGAQSGLSATEFMLSTFLPGRVFSFQSLWFEGRLVTSAA
                     VERMESLFSSNGLPTLPASGPSVARTVHRDDVNAIAGSAVRALSPRPHGPFSVLLRED
                     SQGIPCVTDVLAGRYPATIDFLAAAGMNLPRLQMSLAYGTVTPALPRFDAVEDELYWV
                     RGVDRKPHLFKGAPWVHQRAA"
     gene            2577..3416
                     /locus_tag="AC16YU_12250"
     CDS             2577..3416
                     /locus_tag="AC16YU_12250"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6066042.1"
                     /translation="MLHRIHTPDSGAYLATYRPATPSFTAHARMSRSLVPVWQALHDG
                     DPDGAFRLAMAALEAQRSPSATEKAGLLAGLAGAEYVSGRLDHALRNAQRSLELVPAQ
                     WTARRLVVCIRSRRQEYREAYAGLAELPMDLNGPSWDERLSPRDVQIGLASCAWALGQ
                     WGLVTEHLEQAYPGGPATMPEPEREDSFRVRLFLNRPDLAAEVAALLIANWSPERSDE
                     LLQTLVQRRFTAQALPLYRAVFAMYPGSELIRRRLVALCIKEGAVDEARRLVAPSALR
                     TAA"
     gene            3449..4351
                     /locus_tag="AC16YU_12255"
     CDS             3449..4351
                     /locus_tag="AC16YU_12255"
                     /inference="COORDINATES: protein motif:HMM:NF020252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HIR6066043.1"
                     /translation="MNPDRGSPGLGHLADAAPPLPSTIVRARRLMDRTDGVSPEQVLD
                     AVRHDPVVVSRILRVANAAHYGTQRTVHGIGRAIQLLGPMTVLGIVTGMHIVSSPSPA
                     AAPSGYCFLRLVRHSVATGYLVEALNAHLPAPFPPDMAYTAGLLHDLGRILLASHDPE
                     GARSVYADPGTVATGSAEELLALEQVAFGFDHTEAGEYVARRLDFPELLVSAIRNHHR
                     PEGGSPNREAVRLARLVGLADAAASSLGYPARDERPADTFDGHAAWTALGFADAAAAL
                     AATRARAASMSGVVDGGLGIRSDT"
     gene            complement(4386..5792)
                     /locus_tag="AC16YU_12260"
     CDS             complement(4386..5792)
                     /locus_tag="AC16YU_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6066044.1"
                     /translation="MSRQPHILFVDDEQLLHSLFERLFARHGMKVTSCLSATRAVELL
                     EGTEFDLVVTDFMMPDMDGFALLGHIRQHHPRTRVIMITAHANVQHAVRMMQHGAIDY
                     IPKPFSTAELVERVQASLAREAPGTADPGEVAPPDAAPARGARARKSQVAYIGDAPAI
                     RRLMDVLPRVASNRAPVFIQGESGTGKEILARLIHQMSDRSGGPYLTINCANLPRELV
                     ESHLFGHKKGSFTGAIEDMVGAFDRADGGTLLLDEITEIDLSVQAKLLRVLQESEFQR
                     IGSNDVHKVDVRVIATSNRNLAETIALGQFREDLYHRLAVFPLTVPPLRDHPTDIPLL
                     AGHFVTRYCALYDLQPKKLAGPLLERFQGYSWPGNIRELENLVQRGVLLSAERGTIEA
                     GDVYDDFFRDADPTRAAQAGSPDPKAQTIEDMERFMILQALEDTGNNQQMAAQKLGIS
                     ARTIRNKMKKYREDGLLD"
     gene            complement(5917..6660)
                     /locus_tag="AC16YU_12265"
     CDS             complement(5917..6660)
                     /locus_tag="AC16YU_12265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6066045.1"
                     /translation="MLTRHQASRRADRIPEPPIRGAVDGVSSIHLANVLTHRLGSLVS
                     GIAGFTELLLERLGESDQRELAMRIMESALRIEHVLADLTHYTRPISPEIEPVPVATL
                     FDDVLRVLPDEVAERIVVDAGDLGARNVVADPYLARDVLTYLLLNAIEATPGKEPVTL
                     RASPGVPASVLWLEVENPASLEPAIAGRMFEPFFTTKARNLGVGLPLARRYARMQLGD
                     VTLAADGLDGRVVLRFSLPASRLDPESAP"
     gene            7110..7385
                     /locus_tag="AC16YU_12270"
     CDS             7110..7385
                     /locus_tag="AC16YU_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066046.1"
                     /translation="MRSLFLQFSVLIGALTLANRIAGEATATEAALAALGAATAMYIV
                     LVVGDVVVQRAVDYLAPAPPEAIPAARPSADASSAAPSVETPSALAA"
     gene            7432..8193
                     /locus_tag="AC16YU_12275"
     CDS             7432..8193
                     /locus_tag="AC16YU_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6066047.1"
                     /translation="MSQDLQLFAVRYTGDPSIDNRESVVVAALPLVRSIVARLSIPDH
                     PLAAREDLENVGLLGLLQALDGYDPDRGTPFVSYAYGRIRGALVDYLRSIDALPRERR
                     RRLAEAHQAMDVLRQKLGGEPADLEVANHLGMTLVEYHTLLRDAQSRFALSLHAPVDR
                     EGEQTVLESIPNTDTTEAFDLIDRTSLKDYIQTLINDLPEREQTIIALYYFENLTLRE
                     IAVLMDRTEARISQILGKVLKQLREHLARVQHRAA"
     gene            8358..10442
                     /locus_tag="AC16YU_12280"
     CDS             8358..10442
                     /locus_tag="AC16YU_12280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6066048.1"
                     /translation="MNRLRESTGVILWILVFAFGVIWVLQDSGGLDVVGVLSNNVGTV
                     NGEPISFEEYSRALDTQVQNYQEQNGESMPPQMLDQTRNAVFDQLVELKLREQELERL
                     GLQVSDQEVVEMVQGENPHPIIRLYFGDGQGGVDRALLQSVIENPEARADWLNIENYL
                     RAERLREKLDNLITAGFHVSDADVEGEYLRRSRTVDVSFVALRFASIPNDSVQVTDSD
                     LRAFYDRNREDFARKRSYTFRYASEAKSPTPEDTAHVLDDLEGMRDAFAAAESDSTYL
                     VRNGSSTAYADAYFRPDELDPAVSDVVFDNPVPGTVVGPVIGGGFAHLVKIIDVRAPE
                     ETAVRARHILFRAAENDPAARQEAETQAREVLQRIRGGEDFAEMARTYSSDGTAANGG
                     DLGWFGPGRMVEPFEEAAFGARVGQPVGPVATQFGFHLIEVTERATVEAKVADFTLPL
                     QPSVATLNRIQEQLDDLQYFSTENGDFSGEAQRRGLEVTTVEIEEEQDFIPGIGASTT
                     LVNFLADAEAGSVSPVIELDTRFIVGVVDAVQPAGYRPLEEVRSQLEPRVRNEKKAAQ
                     LKARLQAAVASSSDLEGIAQVVGQPVATANSLSFQNLVVPTLGRDPGFVGAALGLEEG
                     SLSGVIEGTSAVYVIRADQVTEPAPISDAQRSALRNQILAQGRDRLRTQWITDLREKA
                     EIVDNRRALLVQ"
     gene            complement(10511..11314)
                     /gene="dapF"
                     /locus_tag="AC16YU_12285"
     CDS             complement(10511..11314)
                     /gene="dapF"
                     /locus_tag="AC16YU_12285"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6066049.1"
                     /translation="MTGAGNDFIVIDNRIYRFAVGALALLARTFCPRRTGVGADGLLA
                     LEAVDGPDTHFRMRYHNADGSVGTMCGNGARCLARYAYRSGIRHRPLRFVTDAGIYEA
                     DVPDDAEAPVRLFLPAPRGYRVLERGLHPDLPDGGHFIWTGTEHVVVFVPSVDSAPVE
                     TAGPELRAAREVSGSGANVDFVEVAGPEELRVRTWEKGVEGETLACGTGATAAALVAC
                     LSGRIPAGRIRVRMPGGDLAVGVEGDPAEPDRLYLEGPAETVFHGTLAA"
     gene            11401..12369
                     /locus_tag="AC16YU_12290"
     CDS             11401..12369
                     /locus_tag="AC16YU_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6066050.1"
                     /translation="MSEKRIRIEKAEPLLLFGFNDVYLRRIEQAFPDVQITARGDQLI
                     LQGEDPSMDRIARVVDELVLLVNRNGHLTENDVDTVLALFGRGDGAPPESPDSQHTVV
                     FTPSGGSVRAKTPNQIQLVESARTQDIVFAIGPAGTGKTYTAVALAVAALKSRQVKRI
                     VLCRPAVEAGERLGFLPGDFREKIDPYLRPLYDALEDMHSREKLLSLMEQNIVEIAPL
                     AYMRGRTINAAFVILDEAQNATALQMKMFLTRLGAASRAIITGDITQTDLPVREQSGL
                     VQARDILEGVEGIDFVYFDERDVIRHRLVKDIIKAYERYHGGEDAG"
     gene            complement(12391..13410)
                     /locus_tag="AC16YU_12295"
     CDS             complement(12391..13410)
                     /locus_tag="AC16YU_12295"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR6066051.1"
                     /translation="MSAARSIAFLLILAAPASVGAQHRFPIEIDGRTGFIDASGAVAI
                     EPRFEAGEVFSDGLGAVRVDGLWGFIDPAGAWAVEPGFLEAGAFNEGRAPVRDSLGWW
                     LIDRQGTRLTGHAYPELRAFSEGRALFRAPKGTARPGKAGDWGMLDGSGREVVPAVFA
                     GAMDFSGGLVPVQDWRRFLVFKLERLWGYADSTGAVVIPTRYTSAWPVSDGLAFMTRD
                     GRGLYLDTRGNLVRESTHPQASPYHDGLSRVGDGQRWGFVDREGAVRIELRYEGARDF
                     SEGLAAVREGGLWGFVDTDGRMAIPARYEQAGSFRGPLAAVHLEGRPAWIDREGRIVW
                     TTGAR"
     gene            complement(13407..13805)
                     /gene="panD"
                     /locus_tag="AC16YU_12300"
     CDS             complement(13407..13805)
                     /gene="panD"
                     /locus_tag="AC16YU_12300"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR6066052.1"
                     /translation="MTITMLRAKLHRVRVTQAELYYEGSITVDQELLQAAGILPYEKV
                     AVVNVNNGARFETYTIPAPPGSRTICMNGPAARLASVGDEVIVIAYADMTPEEARNHV
                     ARVLLVDGNNDPVELRRLSVELALQEAADA"
     gene            complement(13865..14728)
                     /gene="panC"
                     /locus_tag="AC16YU_12305"
     CDS             complement(13865..14728)
                     /gene="panC"
                     /locus_tag="AC16YU_12305"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6066053.1"
                     /translation="MQLIRTVPDMQAAADRLRADGRRLVLVPTMGSLHEGHLALVRAA
                     RAEGDHVTVSIFVNPTQFGPGEDFDRYPRDLDGDLDRLEAAGGADAVFAPEVDALYPD
                     GSHGLTWVTVDRMGDHLCGARRPGHFRGVATVVAKLFLACRPHVAVFGLKDAQQFFIL
                     RRMVVDLGFGVRLVGVETVREADGLALSSRNRYLDPPERAEAVVLSRAVAAARELLAG
                     GERDAAAVTARMQAEIAKARRGRLDYAELVDTRDLQPVVGLRPGMTVLAAVAVHFGRT
                     RLIDNAIVDLR"
     gene            14876..16276
                     /locus_tag="AC16YU_12310"
     CDS             14876..16276
                     /locus_tag="AC16YU_12310"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR6066054.1"
                     /translation="MADFRVERDSLGEVRVPADAAYGAQTQRAADNFPISGIRFSRRF
                     IEALGIVKWACAQANRDMGLLDADKADVISSVTERVIEGELDDQFVLDIFQTGSGTSS
                     NMNANEVIANLATESMGGDRGSKLVHPNDHVNMSQSSNDVIPAAMHVAARVAIDRELV
                     PALDRLATALEAKATQFDDVVKSGRTHLMDATPVRLGQEFGGYAAQVRNGLRRLEAAA
                     AELDELALGGTATGTGINCPVGFPARAIRHIATKTALPFREAENHFEAQAAKDAYVFV
                     AGALNALAVGLLKIVNDIRHLASGPTSGLAEIRLPAIQPGSSIMPGKVNPVMSEAMMM
                     VCARVMGNHTTVTIAGQHGNFELNVMMPVMTHAMLESISILAGACDAFRTRALEGIEV
                     DRERCRELLELNPSIATALNSAIGYDKAATVAKKSAAERRSVRDVAKEMGLLSDEELD
                     QVLNVRDMTEPGVPGH"
     gene            16347..>16633
                     /locus_tag="AC16YU_12315"
     CDS             16347..>16633
                     /locus_tag="AC16YU_12315"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6066055.1"
                     /translation="MARRPVIGLTTSLEEGQQRLDLRYVAAVERAGGIPVILPTAQSE
                     ATAVAVADMIDGLVIPGGPALTLGLDGTLPADIDEPDPRRVASDRWILAHFR"
BASE COUNT         2613 a         5959 c         5608 g         2453 t
ORIGIN      
        1 ggatcgggca cggacccggc ggcaacgacg ggcccgagcg gcgcgaacga gtcgaatccc
       61 ttcgccaccg cccagggatg ccccttctcc ttggcccgcg cctgcaggtc gcgcgccgtc
      121 atgtccagcc ccacggcata cccggccacg tgggagagcg cctcggactc cggaatccac
      181 cgaccgccgc gccccacgac gacgacgagc tcgacctcgt gatggacttc gccggagact
      241 ccgggaagga ccacctcgcc gccgtcccgg accaccgccg tcgaaggttt caagaagagc
      301 atgggctcct ccggcggagc gctccgcatt tcggccgcgt ggcgcgcgta attctggccg
      361 atgccgatga tcttgcctat ggcgacgggt tcggcgcgac cgggaacgac gagactcggc
      421 atcgacggcg tttgatttga ggcttgatga attcgtatat tttcaagctg cgtcaagata
      481 cgacgtcagc gtttcactct cgcgccggta gccaacccgg cgctccaaag cagagcagat
      541 tccatgtttg cgatcatcga gatcggcggc aagcagtaca aggtccgcga gaacgaccgg
      601 atctatgtgc cccgccagga cgccgagccc gaggccacgc tcaccttcga ccgcgtgctc
      661 ctggtctcga acgacgacgc ggtccaggtc ggcacaccgg tcgtggacgg ggcctcggtc
      721 gacgcgaccg tcctcgccca ggtcaaggcc gacaaggtgc tcatcttcag gaagaagcgg
      781 cgcaagcgat tccgcgtgaa gaggggccat cgccagccgt atacgcagat ccggatcgac
      841 gcggtcaagg cctgaccgcc cgttcccacg aatcccagat ccgagagaga ccatggcaca
      901 caaaaaagga atggggtcga ccaagaacgg tcgtgactcc cagcccaaga tgctcggcgt
      961 gaaggccttc gccggagagc acgtccaggc cggcgcgatc atcgtgcgcc agcgcgggac
     1021 caagttccac ccggggacca acgtcggccg cggcaacgac gacacgcttt tcgccacggc
     1081 caccggtacg gtccgcttcg cgcgcggccg caacgaccgg cgattcgtgc acgtggatcc
     1141 ggcccaggcc tgatccgcga cccctttccg cctggcaggg gctcccgccg caaggccgga
     1201 gcctctcttt ttttgcgccc ggacggcctc aggccgcgcg ctggtggacc cagggggcgc
     1261 ccttgaacag gtggggcttg cggtccactc ctcggaccca gtagagttcg tcctcgaccg
     1321 cgtcgaagcg cggcagggcc ggcgtgacgg tcccataggc caggctcatc tgcagcctgg
     1381 gcaggttcat gccggcggcc gcgagaaagt cgatcgtggc cgggtaccgg cccgccagga
     1441 cgtccgtgac gcagggaatc ccctggctgt cctcgcgaag cagcacgctg aacgggccgt
     1501 gaggcctcgg gctgagcgcg cgtacggcgc ttccggcgat ggcgttcacg tcgtcccggt
     1561 gcacggtgcg tgcgacggac gggcccgatg cgggcagtgt cggcagcccg ttcgacgaga
     1621 acaggctctc catgcgctcc acggcggcgg acgtgacgag tctcccctcg aaccagaggc
     1681 tctggaagga aaagacacgg ccgggcagga acgtcgacag catgaactcg gtagccgaca
     1741 ggccggactg cgctccccag taggtgagcc acgccgagat ctgctctccg gtcccgaccg
     1801 ggagcgaccc gatgccggtc cgggactgga tggtcctgac gcaggcccgg cccgatcgct
     1861 ccgccacttc ggcgagcgcc tgggccgtgt cgatgttctg gtgcagccgc acgacggtcg
     1921 gcacggggac gtcggccgcg gtcagcaggc ggttggtgtg caggcggttt ctcgccgcca
     1981 ccaggtcggc atgatccgga atcaggagga gccccgggag ctcgtcgcga tgctcggaca
     2041 ggaaggcgag ttccgcatcg ggctgcggat gcacgtacgt cacccgctcg tcggaaacga
     2101 tgccgtgcag cgcttcgaga aatcccggct cggacggctg cggcagcagg tacgaggcat
     2161 ccacgtcggc gcaggccagg tgaaacgcgt tcgtgtcggc gccgacgacg aacgcggtct
     2221 cgtcggccat gcggagggcg gcgacgacgt tgcggccggc gttgctgccg gcaccggtga
     2281 cgaggatgcg cttcatgggt accaggggac ggggacggct tcggtcgttc agtctggccg
     2341 ggatgcacgc ggcgtgccaa gccgggtatc ggccgaacgg ccccttcccg aagcgcccgg
     2401 aggctccccg cgggaaaatc cggccgcgcg gcgcggcaca ttctgcccga aatccagtcc
     2461 gccggggccc cacatccacc cccttggcgc attcgggctt tggcagaggg tttgcagccg
     2521 atggctcgtc ggccgatcga cgggccacat cccctcgccg ccatcccctc ggacccatgc
     2581 ttcatcgaat ccatacccca gactcgggag cctacctggc aacgtatcgc ccggccacgc
     2641 cctcgttcac ggcgcatgcc cgcatgagcc gatccctggt cccggtctgg caggccctgc
     2701 acgacggtga cccggacggc gcgttccgcc tcgcgatggc ggccctcgag gcccagcgca
     2761 gcccctcggc gacggagaag gcgggcctgc tcgccggcct cgccggcgcg gagtatgtca
     2821 gcgggagact ggaccatgcg ctccgcaacg cccaacgctc gctggaactg gtccccgcgc
     2881 agtggaccgc gcgccgcctc gtcgtctgca tccggtcccg gagacaggag tatcgggagg
     2941 cctacgccgg gctcgcggag ctgccgatgg atctgaatgg cccgtcgtgg gacgaacgcc
     3001 tctcgccgcg cgacgtgcag atcggactcg cctcctgcgc ctgggcgctt ggccagtggg
     3061 gcctggtgac ggagcatctc gagcaggcgt accccggcgg ccccgccacg atgcccgaac
     3121 ccgagcgcga ggacagcttc cgcgtgcggc ttttcctgaa ccgcccggac ctggccgccg
     3181 aggtggccgc gctgctcatc gccaactggt cgcccgaacg ctcggacgag ctattgcaga
     3241 ccctggtgca gcgccgcttc acggcgcagg cccttcccct gtaccgcgcc gtattcgcga
     3301 tgtatcccgg aagcgagctc atccggcgcc gactcgtggc cctctgcatc aaggagggcg
     3361 ccgtcgacga ggcacgccgc ctcgtcgctc cgtcagccct ccgaacggcc gcctgaccgg
     3421 caacccgatg cagggaaccc cgcaccccgt gaaccccgac cgtggatcgc ccggactcgg
     3481 ccatctcgcg gatgcggctc cgcccctgcc ctcgacgatc gtgcgggccc gccgcctgat
     3541 ggaccggacc gacggcgtat ccccggagca ggtcctggac gcggtccggc atgatcccgt
     3601 ggtggtgtcc cggatcctga gagtggccaa cgccgcgcac tacggcacgc agcgtaccgt
     3661 tcacggcatc ggcagggcca tccagcttct cgggccgatg accgtgctcg gcatcgtgac
     3721 gggcatgcac atcgtctcca gtccgtcgcc cgccgcggcg ccgtccggct actgcttcct
     3781 gcgcctcgtg cggcattccg tcgcgacggg gtacctggtc gaggccctca acgcgcacct
     3841 gccggccccc ttcccgcccg acatggccta taccgcggga ctccttcacg acctcggacg
     3901 gatcctgctc gccagccacg atcccgaggg cgcccgcagc gtgtacgcgg accccggcac
     3961 cgtcgcgacc ggaagcgcgg aggagctcct ggccctcgag caggtggcct tcggcttcga
     4021 ccacaccgag gccggcgagt acgtggcccg ccggctggat ttccccgaac tgctcgtgag
     4081 cgcgatacgc aaccatcacc ggcccgaggg cggctcgccg aaccgggagg cggtccggct
     4141 ggcgcgactg gtcggcctcg ccgacgccgc cgcctccagc ctgggctatc ccgcccggga
     4201 cgagcggccg gccgacacgt tcgacggcca cgcggcctgg acagcactcg gattcgccga
     4261 cgccgctgcc gccctcgccg cgacccgggc gcgcgctgcg tcgatgagcg gcgtcgtgga
     4321 cggcgggctc gggatccgga gcgatacctg acccgtgccc gatgcgactt cggcgaggcc
     4381 gccgatcagt ccagaagccc gtcctcccgg tacttcttca tcttgttccg gatcgtccgc
     4441 gcgctgatgc ccagcttctg cgcggccatc tgctggttgt tgcccgtgtc ctcgagcgcc
     4501 tgcaggatca tgaaccgctc catgtcctcg atggtctgcg ccttgggatc gggcgagccc
     4561 gcctgggccg cccgggtggg gtcggcgtcc cggaaaaagt catcgtacac gtcgcccgcc
     4621 tcgatcgtcc cgcgctcggc cgagagcagg accccgcgct gcacgaggtt ttcgagctcg
     4681 cggatgttgc cgggccagga atatccctgg aaccgctcca ggagcgggcc ggcgagcttc
     4741 ttcggctgca ggtcgtagag cgcgcagtac cgggtcacga agtggccggc caggagcggg
     4801 atgtccgtcg ggtggtcgcg cagtggcgga accgtgagcg gaaacacggc gagccggtga
     4861 tacaggtcct cgcggaactg gccgagggcg atggtctcgg cgaggttgcg gttgctggtc
     4921 gcgatcaccc gcacgtccac cttgtggacg tcgttcgagc cgatccgctg gaactcggac
     4981 tcctgcagca cgcgcaggag cttggcctgc acggacaggt cgatctcggt gatctcgtcc
     5041 aggagcagcg tgccgccgtc cgcccggtcg aacgcgccga ccatgtcctc gatcgcgccc
     5101 gtgaaggagc ccttcttgtg cccgaagagg tggctctcga cgagctcgcg cggaaggttc
     5161 gcgcagttga tcgtgaggta gggcccgccc gagcggtcgc tcatctggtg gatgagtcgt
     5221 gcgaggatct ccttgcccgt cccagactcc ccctggatga acacgggagc ccggttggac
     5281 gccacgcggg ggagcacgtc catgagccgg cggatggcgg gtgcgtcgcc gatgtacgcg
     5341 acctggctct tgcgggcgcg cgctccccgg gcgggcgccg cgtccggcgg cgccacttcg
     5401 cccgggtctg cggtccccgg cgcctcgcgg gcgagcgagg cctgcacccg ctccacgagc
     5461 tccgccgtgg agaagggctt cgggatgtag tcgatcgccc cgtgctgcat catccggacc
     5521 gcgtgctgca cgttcgcgtg cgccgtgatc atgatgacgc gggtccgcgg atggtgctgg
     5581 cggatgtgcc cgagcagggc gaatccgtcc atgtccggca tcatgaaatc ggtgaccacg
     5641 aggtcgaact cggtgccttc caggagttcc accgcgcgcg tcgcgctcag gcagctcgtc
     5701 accttcatgc cgtggcgggc gaacagccgc tcgaacaggc tgtggagcag ttgctcgtcg
     5761 tcgacgaaca ggatgtgcgg ctgccgactc atgcagggtg cgggggctcg gtgctggcgt
     5821 cgaatatacc caccgcgccc ccttcgagca cgaagcccga accctccggg ccgtgtgaac
     5881 aggccccgtc cgaggcttac ttcttcagca taccgtctac ggcgccgatt cgggatccag
     5941 gcgggacgcc ggaagcgaga atcggagcac cacccggccg tccaggccgt ccgcggccag
     6001 ggtaacgtcc ccgagctgca tgcgggcata tcgcctcgcc agcggcaggc ccacgcccag
     6061 gtttcgggcc ttggtcgtga agaacggctc gaacatccgg ccggcgatgg ccggctcgag
     6121 cgacgccggg ttttcgacct cgagccacag gaccgacgcg ggaacgcccg gtgatgccct
     6181 gagggtcacc ggctccttcc ccggcgtggc ttcgatggcg ttcagaagca ggtacgtgag
     6241 gacgtcgcgt gcgaggtacg ggtcggccac cacgttccgg gccccgaggt cccctgcgtc
     6301 caccacgatg cgctcggcca cctcgtcggg cagcacgcga agcacgtcgt cgaacagggt
     6361 cgcgaccggc accggctcga tctcgggcga gatcgggcgg gtgtaatgcg taaggtcggc
     6421 aagcacatgc tcgatgcgca gagcgctctc catgatccgc atcgccagct cccgctgatc
     6481 cgactccccc agccgctcga gcagaagctc cgtgaatccg gcaatgccgg aaaccaggct
     6541 gccgagcctg tgggtgagca cgttcgcgag gtggatggac gatactccgt ccaccgcgcc
     6601 ccggatcggg ggctcgggga ttcggtcggc gcggcgggat gcctggtggc gggtcagcat
     6661 ggcggagtcc gggagttgac ccgaaccgat tcaaaccgcg tgccgccggg ccaaattggg
     6721 gctcgcgacc tcgtttaacg ccaccgggcg cctcccgcgg atcgcgccgg ggcacttccg
     6781 gctgctcgcg ggaggaactc cttgcacggg ggaccggcag ctttttcctc cggaggggtg
     6841 caggaatcgc gtcgcggcgg gatgcgggtg cacgggggaa tcgaccggaa accgccctcg
     6901 cgacgcagca gccacgccct cggagcggct gaagaaaagc ggcccgcatc ggcagtaatc
     6961 ggccctttgg cgatttccct taagcggggc cgctgccgcc gccggacatc cggcgccgac
     7021 tccgaccgcg atgaagagtg ccccgggcgc cggtgcggca cgccgcttgc tcaccccgct
     7081 gccagagtcc gaaccatccc ccgaaacccg tgcgatcact cttcctgcag ttcagcgtgc
     7141 tcatcggcgc cctgaccctt gccaaccgaa tcgccggcga agcgaccgcg accgaggctg
     7201 ccctggccgc tctcggcgcg gcgactgcga tgtacatcgt gctcgtcgtg ggcgatgtcg
     7261 tcgtgcagcg cgccgtcgac tacctggccc ccgcgccacc cgaggccatc ccggccgcga
     7321 ggccgtccgc cgacgcctcg tccgccgccc cttccgtcga aactccgagc gccctcgccg
     7381 cctgagcctc tctcgcacgg ccgcgcgccc cccttacccc caaaccaagc catgtcccag
     7441 gatctacagc tgttcgccgt ccggtacacc ggagacccct cgatcgacaa ccgggaatcc
     7501 gtcgtcgtcg cggcgcttcc cctggtccgt tcgatcgtcg cccgcctcag cattcccgac
     7561 catcccctcg cggcccggga agatctcgag aacgtcggcc tcctcggcct cctgcaggcc
     7621 ctcgacggct acgaccccga ccgggggacc ccgttcgtct cgtacgccta cggtcgcatc
     7681 cggggcgccc tcgtggacta cctccgctcg atcgacgccc tccccaggga gcggcggcgc
     7741 cggctcgcgg aggcgcacca ggcgatggat gtgctccgcc agaagctcgg cggagagccg
     7801 gccgacctgg aggtggccaa ccacctgggc atgaccctgg tcgagtatca cacgctcctg
     7861 cgggacgcac agtcccgctt cgccctttcg ctccacgccc cggtcgaccg cgaaggcgag
     7921 cagaccgtgc tcgaatccat ccccaacacg gatacgaccg aggccttcga cctcatcgac
     7981 cgcacctcgc tgaaggacta catccagacg ctcatcaacg accttcccga gcgggagcag
     8041 acgatcatcg ccctgtacta cttcgagaac ctgaccctgc gcgagatcgc ggtgctcatg
     8101 gaccgcaccg aggcccggat ctcccagatc ctgggcaagg tcctgaagca gctccgcgag
     8161 cacctggccc gggtccagca ccgcgcggcg tagggcgatc cgggatggca attgcggcac
     8221 aagggctcga acaacctcgg gcccttgccg tatattgcag gtctttgtag gcccggccca
     8281 caggcctacg gggtggtccg cagggaccgc ccgcgattct ccatccacgc attggttgag
     8341 gttggttcat gggtgtgatg aacaggctcc gcgagagcac cggggtcatc ctgtggattc
     8401 tggtattcgc cttcggcgtc atctgggtgc tccaggactc cggtggcctg gacgtggtcg
     8461 gcgtgctgtc gaacaatgtc gggaccgtga acggcgagcc gatctcgttc gaggagtaca
     8521 gccgtgctct cgatacgcag gtccagaact accaggagca gaacggcgag agcatgccgc
     8581 cgcagatgct ggaccagacc cgcaacgccg ttttcgacca gctggtcgag ctcaagctcc
     8641 gcgagcagga actcgagcgc ctgggcctgc aggtgtccga ccaggaggtc gtcgagatgg
     8701 tccaggggga gaacccccat ccgatcatcc ggctgtattt cggtgacggg cagggcggcg
     8761 tggaccgggc gctgctgcag agcgtcatcg agaatccgga ggcgcgggcc gactggctca
     8821 acatcgagaa ctacctgcgc gccgagcggc tccgcgagaa gctcgacaac ctcatcaccg
     8881 cgggcttcca cgtctcggat gccgatgtcg agggagagta cctccggcgc agccggaccg
     8941 tcgacgtgag cttcgtcgcg ctgcgcttcg cctcgatccc gaacgactcc gtccaggtca
     9001 ccgattcgga cctgcgggcg ttctacgaca ggaaccggga ggacttcgcg cgcaagcggt
     9061 cgtacacctt ccgctacgcc tcggaggcga agtccccgac acccgaggac acggcgcacg
     9121 tcctggacga cctcgagggg atgcgcgacg ccttcgcagc ggccgagagc gattcgacgt
     9181 acctcgtccg caacgggtcc tctacggcat acgcggatgc ctatttccgc cccgacgagc
     9241 tggatccggc cgtctcggac gtggtcttcg acaacccggt gcccggcacc gtggtgggtc
     9301 cggtgatcgg cggcggcttc gcgcacctgg tcaagatcat cgacgtgcgg gcgcccgagg
     9361 agaccgcggt gcgtgcccgc cacatcctgt tccgggcggc ggagaacgat cccgcggccc
     9421 gtcaggaggc ggagacccag gcccgcgagg tgctccagcg gatccgcggc ggcgaggact
     9481 tcgccgaaat ggcccggacg tattcgagcg acggcactgc cgcgaacggc ggcgacctgg
     9541 gctggttcgg ccccggacgc atggtggaac cgttcgagga ggcagcgttc ggcgcccgcg
     9601 tcgggcagcc cgtggggccc gtggcgacgc agttcggttt ccacctgatc gaggtgaccg
     9661 agcgggcgac ggtcgaggcg aaggtggccg acttcacgct gcccctccag ccgagcgtag
     9721 ccacgctcaa ccgcatccag gagcagctcg acgacctgca gtacttctcc acggagaacg
     9781 gtgacttcag cggggaggcg cagcgccgcg ggctcgaggt cacgaccgtc gagatcgagg
     9841 aggagcagga cttcatcccc ggcatcggcg ccagcacgac gctggtcaac ttcctggccg
     9901 acgcggaggc cggctcggtc agccccgtga tcgagctcga tacgcgcttc atcgtgggcg
     9961 tggtcgacgc ggtgcagccc gcgggctacc gcccgctcga ggaggtccgc tcgcagctcg
    10021 agccgcgggt gcgcaacgag aagaaggcgg cccagctcaa ggcccgtctc caggccgccg
    10081 tcgcgtcgtc ctccgacctc gagggcatcg cgcaggtcgt cggccagccg gttgccacgg
    10141 cgaacagcct ctccttccag aacctcgtcg tgccgaccct cggacgggat cccggattcg
    10201 tcggggcggc gctcggcctg gaggaaggct cgctctccgg cgtcatcgag ggcacgagcg
    10261 ccgtctacgt catccgcgcg gaccaggtca ccgagcccgc gccgatctcc gacgcgcagc
    10321 gcagtgccct gcgcaaccag atactcgcgc agggccgtga ccgcctgcgt acccagtgga
    10381 tcacggacct ccgggaaaag gccgagatcg tggacaaccg ccgggcgctt ctcgtccagt
    10441 agcggacgcc ttcccagcct cgccgatccg cgcccggtcg tcttcggacg gccgggcgct
    10501 ttcgcgtggg tcaggcagcg agcgttccgt ggaacacggt ctcggcggga ccctccaggt
    10561 acagacggtc cggctccgcg ggatccccct cgacgcccac cgcgagatct cctcccggca
    10621 tgcggacgcg gatccggccg gccggaatcc gccccgagag acaggccacg agcgccgcag
    10681 ccgtggcccc cgtgccgcac gcaagggtct ccccctccac ccccttttcc caggtccgga
    10741 cgcggagctc ctccgggccg gccacctcga cgaagtcgac gttggccccg gacccggaga
    10801 cctcgcgggc cgcgcggagc tcgggcccgg ccgtctccac gggggccgag tccaccgacg
    10861 gtacgaagac cacgacgtgc tcggtgcccg tccagatgaa gtgtcctccg tcgggcaggt
    10921 ccgggtgcag tccccgctcg agcacccggt agccccgcgg cgcgggcagg aagagccgga
    10981 ccggggcctc ggcgtcgtcc ggcacgtcgg cctcgtagat gcccgcgtcg gtcacgaacc
    11041 ggaggggccg gtgacggatg cccgaccggt acgcgtaccg ggccaggcac cgcgcgccgt
    11101 ttccgcacat cgtgcccacc gacccgtccg cgttgtgata gcgcatgcgg aaatgcgtgt
    11161 ccggcccgtc caccgcctcc agggcgagca ggccgtcggc tccgacgccc gtgcgccggg
    11221 gacagaacgt ccgggccagg agcgcgagcg cgccgaccgc gaaccgataa atgcggttgt
    11281 cgatgacgat gaaatcgttc ccggccccgg tcattttcgt aaattcgacg tcgacgcgtc
    11341 gagacccgtc ttccggcatg gatttcaccc accgattggt tacccggtag tttacccacg
    11401 ttgtccgaga agcgcatccg aatcgagaaa gccgagcccc tcctgctctt cggcttcaac
    11461 gacgtttacc tgagacgcat cgagcaggcg ttccctgacg tccagatcac cgcacgcggg
    11521 gaccagctca tcctgcaggg agaggatcct tcgatggatc ggatcgcccg cgtcgtggac
    11581 gagctggtgc tccttgtcaa ccggaacggg catttgacgg agaacgacgt cgacacggtg
    11641 ctcgcgctct tcggccgcgg cgacggcgcg ccgcccgaat cgcccgacag ccagcacacc
    11701 gtggtcttca cgccctccgg cgggtcggtc cgggcgaaga cgccgaacca gatccagctc
    11761 gtcgagtctg cgcggaccca ggacatcgtg ttcgcgatcg gtccggcggg taccgggaag
    11821 acgtacacgg cggtcgcgct cgcggtcgcc gccctcaagt ccaggcaggt caagcgcatc
    11881 gtcctgtgcc ggccggccgt cgaggcgggc gagcggctcg gcttcctccc cggggatttc
    11941 cgggagaaga tcgaccccta cctcaggccc ctgtacgatg ccctggagga catgcactcg
    12001 cgcgagaagc tcctgagcct catggagcag aacatcgtcg agatcgcccc gctcgcctac
    12061 atgcggggcc gcacgatcaa cgcggcgttc gtcatcctcg acgaggcgca gaacgcgacc
    12121 gcgctccaga tgaagatgtt cctgacgcgg ctcggcgcgg ccagccgggc catcatcacg
    12181 ggcgacatca cgcagaccga cctgcccgtg cgagagcaga gcgggctcgt gcaggcccgg
    12241 gacattctcg agggcgtcga ggggatcgac ttcgtgtatt tcgacgagcg ggacgtgatc
    12301 cggcaccgcc tcgtgaagga catcatcaag gcctacgagc gctaccacgg gggcgaggac
    12361 gcgggctgac cgccgcagcc gccggcctgc tcaccgggcg ccggtcgtcc agacgatccg
    12421 cccctcgcgg tcgatccagg ccgggcggcc ttccaggtgc accgcggcga ggggaccgcg
    12481 gaacgatccc gcctgctcgt accgtgccgg aatcgccatg cggccgtcgg tgtccacgaa
    12541 gccccacagg ccgccttcgc ggaccgccgc gaggccctcg gagaagtcgc gggcgccctc
    12601 gtaccgcagc tcgatccgca cggcaccctc gcgatccacg aagccccacc tttggccgtc
    12661 cccgacccgg ctcagcccgt cgtggtacgg cgaggcctgg ggatgtgtcg attcccgaac
    12721 gaggttcccg cgcgtgtcca ggtacaggcc acggccgtcg cgcgtcatga aggccagtcc
    12781 gtccgatacc ggccacgcgg aggtgtaccg ggtcgggatc acgacggcgc ccgtggaatc
    12841 cgcatacccc cacagccgct ccagcttgaa gacgaggaag cgccgccagt cctggaccgg
    12901 gaccagtccg ccggaaaagt ccatcgcccc cgcgaagacc gcgggaacga cctcgcgacc
    12961 cgacccgtcg agcatgcccc agtcgccggc cttgcccgga cgcgcggtcc ccttcggggc
    13021 ccggaacagg gcccgaccct cgctgaaggc gcgcagctcg ggataggcgt ggccggtgag
    13081 gcgggtcccc tgccggtcga tcagccacca tcccaggctg tcacggacgg gcgcacgccc
    13141 ttcgttgaat gctccggcct cgagaaaccc gggctccacg gcccaggcgc cggccgggtc
    13201 gatgaagccc cagagcccgt ccacgcgcac cgcgccgagc ccgtcggaga agacctcgcc
    13261 cgcctcgaag cgcggttcga tggccaccgc gcccgaggcg tcgatgaagc cggtccgccc
    13321 gtcgatttcg atcgggaacc ggtgctgcgc gccgacgctt gccggggcgg ccaggatcag
    13381 gagaaaggcg atcgagcgtg cggcgctcac gcgtccgccg cctcctgcag ggcgagctcg
    13441 accgacaggc ggcgaagctc cacggggtcg ttgttcccgt ccaccaggag caccctcgcg
    13501 acgtggttcc gggcctcctc gggggtcatg tccgcgtagg cgatgacgat cacctcgtca
    13561 ccgaccgagg cgagccgggc cgccgggccg ttcatgcaga tggtccggct tccgggaggc
    13621 gccgggatcg tgtaggtctc gaaccgggcg ccgttgttca cgttcacgac ggccaccttc
    13681 tcgtacggaa ggatgccggc cgcctggagc agctcctggt cgacggtgat cgatccctcg
    13741 taatacagct ccgcctgggt gacgcgcacc cggtgcagtt tcgcgcggag catcgtgatc
    13801 gtcatggcgg cccacctgcg ggtatcgtcc ggtctaattg ccgggctgac cgaaggtttc
    13861 gccttcagcg aaggtccacg atcgcgttgt cgatgagccg ggtccgcccg aaatggacgg
    13921 cgacggcggc caggacggtc attccgggac gcagcccgac gacgggctgc aggtcccgcg
    13981 tgtccacgag ctcggcatag tccaggcgcc cccgccgggc tttcgcgatc tccgcctgca
    14041 tgcgcgcggt gacggccgcc gcgtcgcgct cgccgccggc gagcagctcc cgggccgcgg
    14101 ccaccgcccg cgacaggacc acggcctcgg cacgctccgg aggatccagg taccggttgc
    14161 gcgacgacag ggcgaggccg tccgcctcgc gcacggtctc cacccccacc aggcggacgc
    14221 cgaatcccag gtccaccacc atccgccgca ggatgaagaa ctgctgggcg tccttcaggc
    14281 cgaacacggc gacgtggggt cggcacgcca ggaacagctt cgcgaccacc gtcgccacgc
    14341 cccggaagtg cccgggacgg cgcgcgccgc acaggtggtc tcccatccga tcgaccgtca
    14401 cccaggtcag gccatgcgag ccgtccgggt acagggcatc gacctccggc gcgaagacgg
    14461 cgtccgcgcc gccggccgcc tcaagccggt ccaggtcgcc gtcgaggtcg cgcggatacc
    14521 gatcgaagtc ctcgcccggt ccgaactgcg tggggttcac gaagatcgac acggtcacgt
    14581 ggtccccctc ggcccgcgcc gcgcggacca gcgccaggtg cccttcgtgc agggagccca
    14641 tcgtcggcac gaggaccagc cgccgtccgt cggcccggag cctgtccgcg gcggcctgca
    14701 tgtcgggtac ggttcggatc agctgcacgg catcgccgga tggggccggg aagctacgcc
    14761 aaaccgcccg tcacccccaa cgagcccgta ttcagacaca atccacgagg gcccggaggt
    14821 ggtccgggta tattgacgga cgttcccctg tttctcctgc ttaccgagat cagtcatggc
    14881 cgattttcga gtggaacgcg actcactcgg cgaggtccgg gtgccggccg acgccgccta
    14941 cggggcgcag acccagcgag ccgccgacaa ttttcccatc tccggaatcc gattctcccg
    15001 ccgcttcatc gaggcactcg gcatcgtcaa gtgggcctgc gcgcaggcca accgggacat
    15061 ggggctgctg gatgccgaca aggcggacgt catcagttcc gtcacggagc gcgtgatcga
    15121 gggcgagctc gacgaccagt tcgtgctcga catcttccag acgggcagcg gcacgtcgtc
    15181 gaacatgaac gccaacgagg tcatcgcgaa cctggcgacc gaatcgatgg gcggcgaccg
    15241 gggctccaag ctggtgcacc cgaacgacca cgtgaacatg tcgcagtcgt cgaacgacgt
    15301 catcccggcg gccatgcacg tggcggcaag ggtcgcgatc gaccgggagc tcgttccggc
    15361 gctggaccgc ctcgccacgg cgctcgaggc caaggccacc cagttcgacg acgtggtgaa
    15421 gagcggccgc acccacctca tggacgctac gccggtccgt ctcgggcagg aattcggcgg
    15481 atacgccgcg caggtccgca acggcctgcg gaggctcgag gcggccgccg cggagctcga
    15541 cgagctcgcg ctcggcggca ccgcgacggg cacgggcatc aactgccccg tcgggttccc
    15601 ggcgcgcgcg atccggcaca tcgcgacgaa gaccgccctc ccgttccgcg aggccgagaa
    15661 ccacttcgag gcccaggcgg ccaaggatgc gtacgtgttc gtggccgggg ccctgaacgc
    15721 gctcgcggtg ggtcttctca agatcgtgaa cgatatccgg cacctcgcga gcggccccac
    15781 cagcggactc gccgagatcc ggctgccggc catccagccg ggctcgtcga tcatgccggg
    15841 aaaggtgaac cccgtcatga gcgaggccat gatgatggtg tgcgcccgcg tcatgggcaa
    15901 ccacaccacg gtcacgatcg ccgggcagca cggcaatttc gagctgaacg tgatgatgcc
    15961 cgtcatgacg cacgccatgc tggagagcat ctccatcctg gccggcgcct gcgacgcctt
    16021 ccgcacgcgc gcgctcgagg gcatcgaggt ggaccgcgag cggtgccgtg agctgctcga
    16081 gctcaaccct tcgatcgcga ccgcgctcaa cagcgccatc gggtacgaca aggcggccac
    16141 cgtcgccaag aagtccgcgg ccgagcggcg ctcggtgagg gacgtggcga aggagatggg
    16201 actgttgtca gacgaggaac tcgaccaggt cctgaacgtg cgcgacatga ccgagcccgg
    16261 cgtaccgggg cactgatccg gggagccgtc ccgtgtccgc ggcgggttcg tatgttcgga
    16321 tccgttccgc atccgcgtac ttcggcatgg cacgacgacc cgtcatcgga ctgaccacgt
    16381 ccctcgagga gggccagcag cggctcgacc tgcggtacgt cgcggccgtc gagcgggcgg
    16441 gcggcatacc ggtcatcctg cccacggcgc agtccgaggc gaccgctgtc gcggtagcgg
    16501 acatgatcga cggcctcgtg attcccggtg gacccgcgct cacgctgggc ctcgacggga
    16561 cgcttccggc ggacatcgac gagcccgacc cgcgccgtgt cgcctcggac cgctggatcc
    16621 tggcccattt ccg
//
LOCUS       DBNHQZ010000451         7940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_1218839, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000451 DBNHQZ010000000
VERSION     DBNHQZ010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_1218839"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="AC16YU_12320"
     CDS             <1..1023
                     /locus_tag="AC16YU_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6066056.1"
                     /translation="VTLNGAAVPAEHVAASLAADSLSWSSDDGPSAITVGADENLFQV
                     FQRVRRAGADGFVVVPTAQAEMFDRLRGFLARPSRSLDLAEGANLVVILSDEPDVRSW
                     SVAAAGTVSETPLANVVGVIPGRRADEIVLFAGHYDHVGVVQPVDGDSIANGANDNAS
                     GTTAVIELARHFAARGVPERTILFVAFAAEESGGFGSRHFASQVDPDRIVAMFNIEMI
                     GKPAVEGPNTAWITGFERTDFGPILQRAVEGTEFAFHPDPYPDQDLFFRSDNAMFARL
                     GVPAHSISTTPIDVDPDYHQVTDEVETLDLEHMTNTIRAIAAGAATIISGEETPTRLD
                     PETVER"
     gene            complement(1024..2409)
                     /locus_tag="AC16YU_12325"
     CDS             complement(1024..2409)
                     /locus_tag="AC16YU_12325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6066057.1"
                     /translation="MDTLLSAKAAELKSGFNGDVLIPGDPGYEEARAIWNAMIDKRPA
                     IIARCRGTADVVRTVNFARSEGIPLAVRGAGHNIAGKGTCDGGLLLDLSGMTAVTVDP
                     AARRVTVEGGATLADVDEATLAHGLAVPTGINSTTGIGGLTLGGGFGWLSRKFGMTVD
                     SLESAEVVTAAGDVVKASASEHPDLFWAISGGGGNFGVVTRFGFLAHPVGPDLLSGLF
                     VYPLSEGKSVLQQYREFMVTAPDELSVWVVLRLAPPLPFLPESVHGSKVVVLPLAYAG
                     NPDEGMAHIEPLARFGTVLGSHVGVQPFVDWQKAFDPLLAPGARNYWKSHNCTGLADG
                     LLDAAIDSISKLPSPQSDVLIGALGGAVSRMDPDSAAYPHRDTRFIVNVHGRWDDAAD
                     DERGMRWTRSVFDACAPYASGGVYVNFMSDDEAARVPEAYGASYDRLARVKSRYDPAN
                     LFRTNQNIVPA"
     gene            complement(2576..3217)
                     /locus_tag="AC16YU_12330"
     CDS             complement(2576..3217)
                     /locus_tag="AC16YU_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR6066058.1"
                     /translation="MGTDRETGKSQASHLTLRRSIGTLGILLPVTLAGGQLLVDPEGG
                     LQISISAYYATPMRDVLVGVLFAIGAFLFAYRGYDRHDDLVTDLACICCVGLALFPST
                     SGVGIVEDMHMVLGMALFLLLAYTAAVRFTLTDPNGTPTPAKLRRNRVYVASGVIMIA
                     SIALGIVYYTFFEETVVASLKPIFWLEALALWAVGWSWFVKGETLLKDSVERT"
     gene            3376..3720
                     /locus_tag="AC16YU_12335"
     CDS             3376..3720
                     /locus_tag="AC16YU_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6066059.1"
                     /translation="MPSDDPLDRVFGALSNPTRRAILTRLAEGEATVLELAEPFAMSQ
                     PAISRHLKVLEEAGLVERGRDAQRRPCRLSPGPMNAVIAWLDTFGEPRQARFPRLDLL
                     FEGLDRLEAKLR"
     gene            complement(3742..4500)
                     /locus_tag="AC16YU_12340"
     CDS             complement(3742..4500)
                     /locus_tag="AC16YU_12340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6066060.1"
                     /translation="MDGLGFDGRVALVTGGASGIGEACVHEFARGGARVAIADMDADR
                     GDAVAARVRASGADAVFIVVDVSDPDSVRRMVARTVEAFGRLDIAVNNAGIGGELSPT
                     GEYSIEGWRRVIDVNLNGVFYCMRYEIPQMLEHGGGAIVNMASILGAVGFATASAYVA
                     AKHGLLGLTKVAALEYAAQGIRVNSVGPAFIRTPMVLESMDEQTLTALEGMHALGRLG
                     EPGEVAGLTAFLCSDRASFMTGGYYLVDGGYTAR"
     gene            4651..6144
                     /locus_tag="AC16YU_12345"
     CDS             4651..6144
                     /locus_tag="AC16YU_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR6066061.1"
                     /translation="MSRATGPCPVAAVLIGLLAGCGSPSSDRQADPGFELADSALFSG
                     PGAITNAVADFDNDGDLDLFVGFNGRPNRLYRNDGGRFTDVATALGVADSSATRSVAW
                     GDFDSDGNLDLFVGFGAGETSWSRLFRNDGERFTDEAADRGVTIGGAFRQASFVDFDR
                     DGDVDLFVAFRDRPNALLRNDDGRFTDAAADLGVADDRRSVGAAWFDYDSDGDLDLVV
                     ANMDGDANGLLRNDGGAFVDVAHEAGLADGGRAPGDEASGTVRPTIVDFDNDGRLDVF
                     MANYGPNGLFRNLGSGRFENVATPLGLAVDGRYDTAVWADHDLDGWPDLYVNGTITGG
                     TSYRDYLFRREGDRFVDVTPPLLLDLNADHGATWFDYDRDGDPDLALTGASSDGMHQL
                     IRNSRDGGRSLQVLVLDAAGRYTVAGSEVRLQDSDSGRLLGTRMVDTGSGYNSQGAAP
                     VLFGLPDAGLVDVEVTTLTAHGREITRIPRVDPAAFRGQVLVVRAGR"
     gene            complement(6215..>7940)
                     /locus_tag="AC16YU_12350"
     CDS             complement(6215..>7940)
                     /locus_tag="AC16YU_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 51"
                     /protein_id="HIR6066062.1"
                     /translation="SYAIVLYEAAAEGNLVRVQADNFDGPFDINNGAKLELGSTAKLR
                     TLATYLQVVEALHRQYATVRADRLRAIPVEQNDHITAWALDYLAEHPDASLSGMLEAA
                     LYRRYSASTGESFYTGGSPHTFQNFTAADDSLELPVLEGFRRSTNLVFIRLMRDIVAY
                     HVHRLPGNPAAMLADGADPRRGVYLARFADREGQQFMYSFYKRYRGKTGPEFWNAFRA
                     AHRLTPGRLAWVFRSVMPDAGEPAFAEFMRTYSGPALPNGNSGDLYRNSDPHGLSWQD
                     RSVRAGIHPLELWLMQHLLSHPADSWDDVASAGADVRQEAYGWLLRATAREAQNRRIR
                     TVLEEDAFAAIHSGWRELGYPFASLVPSYATAIGSSADRPDALAVLVGIVLRGGMRYG
                     ALQIDEVHFGVGTPFETHLHADPPPPVRVLSTEVASTLRWCMTEVVEHGTAVRAKAAV
                     RLPDGTVPLIGGKTGTGDNRHDQFGSRGQLIGSQLLNRTATFAFFIGDRIFGVVTAFV
                     SGPEAEDHQFTSSLPTQVLRTLGSTLMPLFEEPPSARAVQQTQPLPLPDNLVGPPPDP
                     VIQLHRPI"
BASE COUNT         1279 a         2688 c         2739 g         1234 t
ORIGIN      
        1 gtcaccctga acggggcggc ggtgccggcc gagcacgtgg ccgccagcct cgccgcggac
       61 tccctctcct ggtcttcgga cgacgggcct tccgcgatca ccgtgggtgc cgacgagaac
      121 ctgttccagg tcttccagcg ggtgcggcgg gctggagcgg acggtttcgt agtcgtcccg
      181 acggcgcagg cggagatgtt cgaccgcctc cgcggcttcc tcgcccgccc ctcgcgctcc
      241 ctggacctcg cggagggcgc gaacctggtc gtcatccttt cggacgagcc cgacgtgcgg
      301 agctggtcgg tcgccgcggc cggcacggtc tccgaaacgc ccctcgccaa cgtggtcggc
      361 gtgatcccgg gccggcgggc ggacgagatc gtcctcttcg ccggtcacta cgaccacgtg
      421 ggcgtcgtgc agcccgtgga cggcgattcg atcgccaacg gcgccaacga caacgcctcc
      481 ggcacgaccg ccgtcatcga gctggcccgt cacttcgcgg cccggggcgt tccggagcgc
      541 accatcctgt tcgtcgcctt cgcggccgag gaatcgggcg gattcgggtc gcgccacttc
      601 gcctcgcagg tcgatccgga ccggatcgtc gcgatgttca acatcgagat gatcggcaag
      661 cccgccgtcg agggaccgaa cacggcctgg atcacgggtt tcgagcggac ggacttcggc
      721 ccgatccttc agcgggcggt cgagggcacc gagtttgcgt tccaccccga cccctacccg
      781 gaccaggacc tgttcttccg gtcggacaac gccatgttcg cccgcctggg cgtgccggcg
      841 cactcgatct ccacgacacc gatcgacgtg gatccggatt accaccaggt gaccgacgag
      901 gtcgagacgc tggacctcga gcacatgacg aacacgatcc gggcgatcgc cgcgggcgcg
      961 gcgacgatca tctcgggcga ggagacgccc acacggctcg atccggagac cgtggagcgg
     1021 tagtcaggcc ggaacgatgt tctggttcgt gcggaagaga ttcgcgggat cgtaccgcga
     1081 cttcacccgg gcgagtcgat cgtagctcgc gccgtaggcc tcgggtaccc gagccgcctc
     1141 gtcgtccgac atgaagttga cgtagacgcc cccggacgcg tacggagcgc aggcgtcgaa
     1201 caccgaccgg gtccagcgca tgccccgctc gtcgtccgcg gcatcgtccc agcggccgtg
     1261 gacgttcacg atgaatcggg tgtcacggtg cgggtacgcc gccgagtcgg gatccatccg
     1321 cgagaccgca cccccgagtg ccccgatcag cacgtcgctc tggggcgagg gaagcttcga
     1381 gatggagtcg atcgccgcat cgagcagccc gtcggcgagt ccggtgcagt tgtgggattt
     1441 ccagtagttg cgcgctcccg gtgcgaggag cgggtcgaac gccttctgcc agtccacgaa
     1501 cggctgtacg cccacgtggc tgccgagcac cgtgccgaag cgggccaggg gttcgatgtg
     1561 agccatgcct tcgtcgggat tgcccgcgta tgcgagcggg agcacgacga ccttcgaacc
     1621 gtgcaccgac tccgggagga agggcagggg aggcgcgagg cgaagcacca cccagaccga
     1681 gagctcgtcc ggagcggtga ccatgaactc gcggtactgc tgcaggaccg acttgccctc
     1741 ggagagcgga tagacgaaga gcccgctcag gaggtcgggc ccgaccggat gggcgaggaa
     1801 cccgaagcgc gtgaccaccc cgaagttgcc gcccccgccg ctgatcgccc agaacaggtc
     1861 gggatgctcc gacgcgctgg ccttcaccac gtctccggcg gccgtcacga cctcggccga
     1921 ctcgaggctg tccaccgtca tcccgaactt gcggctgagc cagccgaagc cgccgccgag
     1981 cgtcaggccg ccgatgccgg tcgtcgagtt gatgcctgtg ggaacggcca ggccgtgggc
     2041 gagcgtcgcc tcgtcgacgt ccgcgagcgt tgcgccgccc tcgaccgtca cccggcgcgc
     2101 cgcgggatcg acggtcaccg cggtcatgcc ggagaggtcg agcagcagcc cgccgtcgca
     2161 cgtgcccttc cccgcgatgt tgtgcccggc accccggacc gcgagcggga tgccctccga
     2221 gcgcgcgaag ttcaccgtcc tgaccacgtc ggccgttccc ctgcaccggg cgatgatggc
     2281 cggacgcttg tcgatcatgg cgttccagat cgcccgggcc tcctcgtacc cgggatcccc
     2341 ggggatcagc acgtcgccat tgaagcctga cttgagctcg gcggccttcg ccgaaagaag
     2401 ggtgtccatg ctcacctctg actggatttg ggtgtacgac cggatgccgg gcggggacaa
     2461 ccgtccggaa tccgacggag cgcgtcggag ccggcctcgg gggtagcagt cccgacccta
     2521 ccctaacgaa ccggcactcg aaaagcaata ctcgctccgg ccgcgcggcg ccggatcagg
     2581 tccgctcgac gctgtccttc agcagggtct ctcccttcac gaaccacgac cagccgacgg
     2641 cccagagcgc cagggcctcg agccagaaga tcggcttgag ggacgcgacc acggtctcct
     2701 cgaagaaggt gtagtacacg atgccgagcg cgatgctcgc gatcatgatc acgccgctgg
     2761 cgacgtacac ccggttccgc ctgagcttgg ccggcgtcgg cgtgccgttc gggtccgtca
     2821 gcgtgaaccg gacggccgcc gtgtaggcga gaaggagaaa gagcgccatt ccgaggacca
     2881 tgtgcatgtc ctccacgatg cccactccgc tcgtggacgg gaagagggcc agccccacgc
     2941 agcagatgca ggccaggtcg gtgacgagat cgtcatggcg gtcatatccc cggtacgcga
     3001 agaggaaggc gccgatcgcg aacagcaccc ccacgagcac gtcgcgcatc ggggtcgcgt
     3061 agtatgcgct gatggagatc tggagcccac cctccgggtc cacgagcagc tggccgcccg
     3121 cgagcgttac gggcagcagg atgcccaagg tgcctatgga gcgccgcagc gtcaggtggg
     3181 acgcctggct cttgcccgtt tccctgtccg ttcccatgcg tgacgatctc gtggagcgat
     3241 cagagatagt gcgccgggag ggatagggtc aagtggacgc cggccgtgcg cgtgcgacgg
     3301 gacggtcgtc gccggccttg acaggacccg ttggatggta tataatgata ttgttataga
     3361 acgaaacagc tatttatgcc ttccgatgat ccactcgatc gggtgttcgg cgcgctgtcg
     3421 aacccgacgc gaagggccat cctgacccgg ctcgccgagg gggaagcgac ggtgctggag
     3481 ctcgcggagc ccttcgccat gagccagccg gcgatctcgc ggcacctgaa ggtgctcgag
     3541 gaggcgggtc tcgtcgagcg ggggcgcgac gcgcagcgga ggccgtgccg cctctcgccc
     3601 gggcccatga acgcggtcat cgcgtggctg gacacgttcg gcgagccccg gcaggcacga
     3661 ttcccgcggc tcgacctgct gttcgagggg ctcgatcggc tcgaggcgaa gctccggtag
     3721 gaggcttgcg ggtccgggct gctaccgcgc cgtgtacccg ccgtccacca ggtagtatcc
     3781 cccggtcatg aacgacgcgc ggtccgagca caggaaggcc gtgagtccgg cgacttcccc
     3841 gggttcgccg agccggccaa gggcgtgcat gccttcgaga gccgtcagcg tctgttcgtc
     3901 catggactcc aggaccatgg gggtcctgat gaaggccggc ccgacggagt tgacgcggat
     3961 gccctgggcg gcatactcca gcgcggccac cttcgtcagt cccagcaggc cgtgctttgc
     4021 cgcgacgtag gccgacgcgg tggcgaagcc cacggcgccg agaatggacg ccatgttcac
     4081 tatcgccccg ccgccgtgct cgagcatctg cgggatctcg tagcgcatgc agtagaacac
     4141 gccgttcagg ttgacgtcga tcacgcgccg ccagccctcg atgctgtact ccccggtcgg
     4201 gctgagctcg cccccgatgc cggcgttgtt gacggcgatg tccaggcgcc cgaatgcctc
     4261 gacggtccgg gccaccatgc gccggacgga gtccgggtcg gatacgtcca cgacgatgaa
     4321 cacggcatcc gcgccggacg cccgcacccg ggccgcgacc gcatccccgc ggtcggcgtc
     4381 catgtccgcg atcgccacgc gggcgccgcc ccgggcgaac tcgtggacgc acgcctcccc
     4441 gatgccggag gcgccgcccg tcacgagggc cactcttccg tcgaatccaa ggccatccat
     4501 gatcatcctc cccgagcgga atacccttgc aggccagccg aaacggggta atcggggccg
     4561 ggagcaccgg cggactcacc cgtacccgga gtatacgtgc gcaggagcgc cgggcgtata
     4621 ctccggagtc gccttcagat acccttcacc atgtcacgcg cgaccggtcc atgccccgtc
     4681 gcggcagtcc tgatcggcct cctggcggga tgtggatcgc cgtcgtccga tcgtcaagcc
     4741 gatcccggat tcgagctggc cgactcggcc ctgttttccg ggcccggcgc gatcacgaac
     4801 gccgtggcag acttcgacaa tgacggggat ctcgatctct tcgtcgggtt caacggacgt
     4861 ccaaaccggc tctatcgaaa cgacggaggc cggttcacgg acgtcgcgac ggcgctcggg
     4921 gtggcagaca gctcggccac ccgatccgtg gcctggggtg atttcgactc ggacgggaat
     4981 ctggacctgt tcgtgggctt cggcgcgggc gagacctcgt ggagtcgcct cttccgcaac
     5041 gacggtgagc ggttcacgga cgaggcggcc gaccggggcg tgacgatcgg gggggcgttt
     5101 cgccaggcga gcttcgtgga cttcgaccgg gatggcgacg tggacctgtt cgtcgcgttc
     5161 cgggaccggc ccaatgcgct cctgcgcaac gacgacggac gcttcacgga cgccgccgcg
     5221 gacctgggcg tggcggacga ccgccgcagc gtgggtgcgg cctggttcga ctacgattcg
     5281 gacggagacc tggacctggt ggtcgccaac atggacggcg acgccaacgg cctgttgagg
     5341 aacgacggcg gcgccttcgt ggacgtggcg cacgaggcag gcctcgccga cggcggccgc
     5401 gcgcccggag acgaggcgtc aggcaccgtg cgcccgacca tcgtcgactt cgacaacgac
     5461 gggcgcctgg acgtcttcat ggcgaactac gggccgaacg gcctcttccg gaacctgggg
     5521 agcgggcgtt tcgagaacgt ggccacaccg ctgggcctcg ccgtggacgg gcggtacgac
     5581 accgcggtct gggccgatca tgacctcgac gggtggccgg acctgtatgt gaacggcacg
     5641 ataaccggtg gcacgagcta cagggactat ctcttccgcc gggaagggga tcgcttcgtc
     5701 gacgtgaccc cgcctctgct gctcgacctg aatgccgatc acggcgccac ctggttcgac
     5761 tacgaccggg acggcgaccc ggacctggcg ctcaccgggg catcctccga cggcatgcac
     5821 cagctcatcc gcaatagccg ggacggaggc cgctccctcc aggtgctcgt cctggacgcg
     5881 gccggccggt acaccgtcgc gggctcggag gtgcgcctcc aggactccga ttccggccgg
     5941 ctgctcggaa cacgcatggt ggatacgggc tccggctaca actcgcaggg cgccgctccc
     6001 gtgctgttcg ggctccccga tgccggcctc gtcgacgtgg aggtgacgac gctcaccgcc
     6061 cacggccgcg aaatcacccg gatcccccgc gtcgatccgg ccgcattccg tgggcaggtc
     6121 ctcgtggtcc gggccgggag gtgagcccgg acaggccggt ggtcccccga ggctccgcga
     6181 tgctcgagcg catcgacgga ttccggatga ccgttcagat cggccgatgg agctggatca
     6241 cgggatcggg cggaggtccg acgaggttgt ccgggagcgg aagaggctga gtctgctgga
     6301 cggcccgtgc gctcgggggc tcttcgaaga ggggcatcag cgtggagccg agcgtccgga
     6361 gaacctgtgt cgggagcgaa ctcgtgaact ggtggtcttc cgcttccggt ccgctcacga
     6421 aggccgttac cacgccgaag atccggtcgc cgatgaagaa ggcgaacgtg gcagtccggt
     6481 tgagcagctg ggagccgatg agctggcccc ggcttccgaa ctgatcgtgc cggttgtccc
     6541 cggtgccggt ctttccgccg atgaggggga ccgtgccgtc cggaagcctc accgccgcct
     6601 tcgcccgcac ggccgtcccg tgttccacca cctccgtcat gcaccaccgc agggtggaag
     6661 ccacctcggt cgacaggaca cggacgggtg ggggcggatc ggcatgcagg tgggtctcga
     6721 acggggtgcc cacgccgaaa tgcacctcgt cgatctgaag tgccccgtaa cgcatccctc
     6781 cacgaagcac gatgccgacc agcaccgcca gcgcatcggg cctgtctgcc gagctgccga
     6841 tggccgtcgc atacgagggt acgagcgacg cgaacgggta cccgagctcc ctccagccgg
     6901 agtggatcgc ggcgaacgca tcttcctcga gcaccgtccg gatccgtcgg ttctgagctt
     6961 ccctggccgt ggcccggagc agccatccgt acgcttcctg tcgcacgtcg gcccccgcgg
     7021 aagcgacgtc atcccacgaa tccgcgggat ggctgagcag gtgctgcatg agccagagct
     7081 cgagcgggtg tatgccggcc cgtacgctcc gatcctgcca ggaaagccca tgcggatccg
     7141 agttccggta caggtccccg gaatttccat tcggaagggc aggaccggag tacgttctca
     7201 tgaactcggc gaatgcaggt tccccggcgt ccggcatcac ggagcggaag acccatgcca
     7261 accgcccggg cgtcaggcgg tgggccgccc ggaacgcatt ccagaattcg ggaccggtct
     7321 ttccccggta gcgcttgtag aacgaataca tgaactgctg cccttcacga tccgcgaagc
     7381 gggcgaggta gacgccccgg cgtgggtccg caccgtcggc gagcatggct gccgggttac
     7441 caggcagccg gtgaacgtgg taggcgacga tgtcgcgcat caggcggatg aaaacgaggt
     7501 ttgtcgaccg ccggaagccc tccaggaccg ggagttcgag gctgtcatcc gccgcggtga
     7561 agttctggaa ggtgtggggg cttccccccg tgtagaagga ctcccccgtg cttgcggaat
     7621 agcgccggta cagggcggct tccagcatgc cggacagcga ggcatcggga tgctcggcga
     7681 ggtagtccag cgcccaggca gtgatatggt cgttttgctc gacgggaatt gcccggagcc
     7741 gatccgcccg aaccgtggca tactgccggt ggagggcctc gacgacctgg aggtaggtcg
     7801 cgagggtgcg cagcttcgcg gtcgaaccga gctccagctt ggcgccattg ttgatgtcga
     7861 acggaccgtc gaagttgtcg gcctgcaccc gaacgaggtt gccctcggcg gccgcctcgt
     7921 acagaacgat cgcgtaggag
//
LOCUS       DBNHQZ010000452         7516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2843151, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000452 DBNHQZ010000000
VERSION     DBNHQZ010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2843151"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC16YU_12355"
     CDS             <1..296
                     /locus_tag="AC16YU_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HIR6066063.1"
                     /translation="WLKGSHARVAACNDCHAPHDNLAAKLAVKGLNGMKHSWAFTTGL
                     FPEPIRATAMNRDVAQASCLYCHGELTHAIQAVSSGDPGCIRCHPSVGHRTRK"
     gene            315..1664
                     /locus_tag="AC16YU_12360"
     CDS             315..1664
                     /locus_tag="AC16YU_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR6066064.1"
                     /translation="MRKNVLILIGAVLATFVIAYVLTDVIEKRSEAVNPFFRVVELAD
                     TTENPETWGKNFPLQYDSYLRTVDMVRTRFGGSDAVLQDPTPDDPRRVTSQSKLEHLP
                     QLKRMWAGYAFAIDFREERGHAYMLEDQLFTGRQQVPQPGTCLNCHASMVVPWLKAGD
                     GDLVRGFETLNPLPYDEARQDVRHPISCIDCHDPESMQLRVTRPAFMEGIRAVKAAQG
                     VADYDVNRDATHLEMRTFVCGQCHVEYYFEGAEKRLTFPWANGLEIDSIYAYYGAAAF
                     RDWTHAETGADMLKAQHPEFETWSMGTHSKAGVACADCHMPYRRVGAQKISDHHVRSP
                     LLDVAASCQTCHRDTEETLLARAEGIQARHATLRDRALDALVDLIDEIMAASEAGVDE
                     ARLAEAREFQRRASFYVDFVEAENSTGFHADQEGARILAESIDYARRGQLALRDGAR"
     gene            1812..2219
                     /locus_tag="AC16YU_12365"
     CDS             1812..2219
                     /locus_tag="AC16YU_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066065.1"
                     /translation="MNAKSLAYGTIAGTVALFVMGYVIYELLLSGTYTANMNGSLNRP
                     EMDMLLILLSTVVYAFLLTYIFTKWAGIRTLAAGATAGAIIGLLFTLSMDLGMYAMMD
                     VMQPVMLVVDPIASAVWTACGGAAIGLALSKAG"
     gene            2381..3136
                     /locus_tag="AC16YU_12370"
     CDS             2381..3136
                     /locus_tag="AC16YU_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066066.1"
                     /translation="MHRHANPALLFAALVLSWAGCSSASRVSSLEPELPIVLDASTDE
                     WGGRMMRVQDGLASIGVQDDPETVFVAFVTRDRSAIQKIVGSGFTVWLDAEGGEERRL
                     GIRFPLGMGAPQGRGPVSEGQGGFRNAHGEQGGPLEVIGEDGRPGRYGPDQADGIRVR
                     AAIEPDNFMYELAVDRRLLGPTGVLGVGFEADEIERPGGDGQGRGTRPGEARQGRGRS
                     ADPGIRGGRGPAGDVGPLKIWARVRLHNRQDSY"
     gene            3250..7239
                     /locus_tag="AC16YU_12375"
     CDS             3250..7239
                     /locus_tag="AC16YU_12375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6066067.1"
                     /translation="MNARRISHYRILEELGRGGMGVVYRAEDTRLGRMVAMKFMADQL
                     ASDDRSQERFLQEARAAAALSHTNIATLYGIEEDGGRRFITMEYVEGRTLTEIIASGV
                     MPVDRALDLAIQMARGLERAHEAGIVHRDIKPANIRVTPRGELKILDFGLAKLAGSAD
                     LSPEGTTVGTAAYMSPQQVLGEEVDSTSDLWSLGVVLYEMLSGRSPFSHQYVQALYYE
                     ILNETAPAVSTLRSDVDPAVDGILERLLAKETRHRYPSASELIDDLVRIRNGLLQPDA
                     TGRSAVDRPVARADSRASGVFRSAERERRQITTLFCEMAGHGELATDLDPEDLEEASD
                     WLDALCRRQVERFGGQAEPAPGGGVRGFFGFPTAHEHDARHAVQCGLAILAEASTARS
                     RKDRTRLSVRAGVHTGMSLVRDRGGRIDIQGGGSDVAARLAECGASGELVVGKATHRL
                     VRDHFETVSLGLRDVRGFTEDQPLFAVTAERTTVAASGAVAGSAPSPLVGRDLELDLV
                     RRRWLAARAGSGQAILLSGEPGIGKSRLVEAFLEDLGSAWHSRLHCSPLDQNTAFHPL
                     ASALRQLVQPEAPGGTGIDPGELDALLGACGVTAREDVALVAELMDVELPSEWPRSAA
                     SPSARRLRLLQVLVQALVMRYRDASGVVIVEDLHWADPSTVEWLDLLVEELPSLPVLL
                     LALTRPPSLPAWLSRTHASEIALGKLTPEDLASITRHRSGDKPLPPEIVRHVAARTDG
                     VPLFAEELTSMILESGLLRDAGDRYELTGPVPEHLIPATLHGSLMARLDQQASARQVA
                     QVGAVIGRRFPYALLRAVEGEGDDEGLTQALNRLVEAELVTRRGFIPEATFEFRHALI
                     RDAAYESLLRKRRRELHERTAVALSEGPFDATPAAVARHYTEAGLPAKALPLRVEAGR
                     TAMRRNANREAVEQLRAGLELLAELDEGPGRDVHELEILSLLGPAVMMTKGYSDPEGE
                     EIFLRIQEICRRVNAMQPLFFSLYGLVSVYLIRGEYDRAFDILARSLETAQEVGETRY
                     LVMSHNATGVARMFAGQPAEALPHLDAVLRLHDNERDADLALLGWGRLDVSAATSRQL
                     ALQLLGDSDGALAQCREALAAAEGSPNHMTVYHAHLYAGLLYLMRREWAACRDVMQAY
                     LPMAIEYGDPFYIAIATVVSSLAHRDPGQADTFRLAGTVLDQMRSAGYALGLGFLLCH
                     QADALLAKGETAEAASVVDDALRHLGERNGEFWEAEVFRLKGAVEAANGRDPEPWIGR
                     ALETSRRQGARWLELRATADLARRRLEAGRADEARTLIRDALAGLAGGDGLPDLEEAR
                     ALAGRLESA"
     gene            complement(7244..>7516)
                     /locus_tag="AC16YU_12380"
     CDS             complement(7244..>7516)
                     /locus_tag="AC16YU_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6066068.1"
                     /translation="DYGSGERGILLYLRSSLTGDEGELIRHYRHANPAFPHQSTADQF
                     FGEEDLEAYRALGFHACDRALAGLVEGLPGDATEPVFDAWRAGAAA"
BASE COUNT         1117 a         2665 c         2652 g         1082 t
ORIGIN      
        1 cctggctgaa gggctcgcat gcccgcgtcg cggcctgcaa cgactgccac gccccgcacg
       61 acaatctcgc ggccaagctc gccgtgaagg gcctgaacgg catgaagcac tcgtgggcct
      121 tcacgaccgg cctgtttccc gagcccatac gggcgacggc gatgaaccgg gacgtggccc
      181 aggcgtcgtg cctgtactgc cacggcgagc tcacccacgc catccaggcc gtctcgtccg
      241 gagatccggg atgcatccgg tgccacccga gcgtcggcca ccggacccga aagtagcctc
      301 ttccacaacg acccatgcgc aagaacgtcc tcatactcat tggcgcggtc ctcgcgacct
      361 tcgtcatcgc ctacgtcctg accgacgtga tcgagaagcg atcggaagcg gtcaatccgt
      421 tctttcgcgt cgtggagctg gccgacacga ccgagaatcc cgagacctgg ggcaagaact
      481 tcccgctgca gtacgactcg tacctccgca cggtcgacat ggtgcgcacg cgcttcggcg
      541 gttcggacgc cgtccttcag gacccgaccc cggacgatcc caggcgcgtc acgtcccaga
      601 gcaagctcga gcacctgccc cagctcaagc ggatgtgggc cgggtacgcg ttcgcgatcg
      661 acttccgcga ggagcgcggg cacgcgtaca tgctcgagga ccagctgttc acggggcgcc
      721 agcaggtgcc gcagccgggt acctgcctga actgccacgc ctccatggtc gtcccgtggc
      781 tgaaggcggg cgacggcgac ctggtcaggg gattcgagac cctgaacccg ctgccctacg
      841 acgaggcccg ccaagatgtg aggcatccga tctcctgcat cgactgccac gaccccgagt
      901 cgatgcagct gcgcgtcacg aggcccgcct tcatggaggg catccgggcc gtgaaggccg
      961 cccagggcgt cgcggactac gacgtgaacc gggacgccac gcacctcgag atgcgcacct
     1021 tcgtctgcgg ccagtgccac gtggagtact acttcgaggg cgccgagaag cggctcacgt
     1081 tcccgtgggc gaacggcctg gagatcgatt ccatctacgc ctactacggc gccgccgcct
     1141 tcagggactg gacgcacgcc gaaacgggag ccgacatgct caaggcccag catcccgagt
     1201 tcgagacgtg gtcgatgggc acccactcga aggccggcgt cgcctgcgcg gactgccaca
     1261 tgccgtaccg gcgggtgggc gcccagaaga tctccgacca ccacgtccgc agcccgctcc
     1321 tggacgtggc cgcctcgtgc cagacctgcc accgggatac ggaggagacg ctgctcgcgc
     1381 gggccgaggg catccaggcc cggcacgcga cgctgcgcga ccgggcgctc gacgcgctcg
     1441 tcgacctgat cgacgagatc atggcggcct ccgaggccgg cgtggacgag gcgcggctcg
     1501 ccgaggcccg ggagttccag cgccgggcct ccttctacgt ggacttcgtc gaggccgaga
     1561 actcgaccgg attccacgcg gaccaggaag gggcgagaat cctcgccgag tccatcgact
     1621 acgcgcgccg gggacagctc gcgctgcggg acggggcgcg ctgatccttg tgccgcatgg
     1681 gagtccggcg tcggggcatc ttgacagtgt ggcctcccgg atcgtaacga gtggccgccg
     1741 ccccttgcgg gcgccggacc ggcggtccgg gcgtccgccg cgacgaccct cacacaaccg
     1801 gagacacgcg aatgaacgcc aagtccctcg cctacggcac gatcgccggc acggtggccc
     1861 tgttcgtcat ggggtacgtc atatacgaac tgctcctctc gggcacgtac acggccaaca
     1921 tgaacggcag cctgaaccgt cccgagatgg acatgctgct gattctgctg tccaccgtcg
     1981 tgtatgcgtt cctcctgacc tacatcttca cgaagtgggc gggaatccgg acgctggccg
     2041 ccggcgccac ggcaggggca atcatcggtc tcctgttcac gctctccatg gatctcggca
     2101 tgtacgcgat gatggatgtc atgcagccgg tgatgctcgt cgtcgacccc atcgccagtg
     2161 cggtatggac ggcgtgcggc ggcgcggcga tcgggcttgc gctgtcgaag gccggctgat
     2221 tcggccgcgg ggcgggatcg gtacctggtg gacgagccgc cgggcgcttc aggtggcctt
     2281 ttctgcgccg atgcactgga caggcccgta ctccggcggg agcaggaaac gccctgaccc
     2341 gccgggcgtc ggatgctccg tcaccagctc cgcgcacgcc atgcaccgcc acgcgaaccc
     2401 cgcgctcctt ttcgcagccc tcgtcctttc ctgggcgggc tgctcgtccg cttcccgggt
     2461 gtccagcctc gagcccgagc tccccatcgt cctcgacgcc tcgacggacg aatggggcgg
     2521 ccgcatgatg cgcgtccagg acggcttggc ctcgatcggc gtgcaggacg acccggagac
     2581 ggtcttcgtc gcgttcgtga cccgcgaccg gtcggcgatc cagaagatcg tcggctccgg
     2641 gttcacggtc tggctcgacg ccgagggcgg cgaggagcgg cgcctcggca tccgcttccc
     2701 gctcggcatg ggcgcgcccc aggggcgcgg gcccgtctcc gagggtcagg gcggcttccg
     2761 caacgcgcac ggggagcagg gagggccgct cgaggtgatc ggagaggacg gcaggccggg
     2821 gcggtacggg cccgaccagg cggacgggat ccgcgtgcgg gccgcgatcg agcccgataa
     2881 cttcatgtac gaactcgcgg tcgatcgcag gcttctgggt ccgaccggcg tcctgggcgt
     2941 aggcttcgaa gccgacgaga tcgagcgtcc cgggggcgac gggcaggggc gcggcaccag
     3001 gccgggcgag gcgcgccagg gtcgcggccg ctcggccgac ccgggcatcc ggggaggacg
     3061 tggaccggcc ggcgacgtcg gcccgctgaa gatctgggcg agagtccggc tgcacaaccg
     3121 ccaggattcc tactgacgag tcgggggagt tgtctgtagg cgccgcaccc tgtacgtttc
     3181 ggggttcgga cgccggcggg ttcgcctgcc ggggcatccg gcccggatcc agctaccccg
     3241 gacttcccca tgaacgcccg ccggatttcc cattaccgga tcctcgagga actcggtcga
     3301 ggcggcatgg gcgtggtata ccgggcggag gacacccgct tggggcgcat ggtggccatg
     3361 aagttcatgg ccgaccagct cgcctccgac gaccgttcgc aggaacgctt cctccaggaa
     3421 gcgcgggccg ccgccgctct cagccacacg aacatcgcga cgctgtacgg catcgaggag
     3481 gacggggggc gccggttcat cacgatggag tacgtcgagg ggcgaacgct cacggagatc
     3541 atcgcctccg gcgtgatgcc ggtcgaccgg gccctggacc tggccatcca gatggcgcgg
     3601 ggtctggagc gggcccacga ggcgggcatc gtccaccggg acatcaagcc ggccaacatc
     3661 cgcgtgacgc cccgcggcga gctcaagatc ctggacttcg ggctggcgaa actcgccggc
     3721 tcggccgacc tctcgcccga gggcacgacc gtgggaacgg ccgcctacat gagcccgcag
     3781 caggtgctcg gcgaggaggt ggactcgacg agcgacctgt ggtcgctcgg cgtcgtgctc
     3841 tacgagatgc tctcgggccg gagcccgttc tcgcaccagt acgtccaggc cctgtactac
     3901 gagatcctga acgagacggc ccctgccgtg tcgaccctgc gttcggacgt ggatccggcc
     3961 gtcgacggca tcctggagcg actgctggcg aaggagacca ggcaccgcta cccgtccgcg
     4021 agcgagctca tcgacgacct ggtgcggatc cggaacggcc tgctgcagcc cgacgcgacg
     4081 gggcggtcgg cagtggaccg gcccgtggcg cgggccgatt cgcgggcgtc gggggtcttc
     4141 cgaagcgccg agcgtgaacg ccgccagatc accaccctgt tctgcgagat ggcggggcac
     4201 ggtgagctgg ccactgacct cgacccggag gacctcgagg aggcgtccga ctggctcgac
     4261 gcgctctgcc ggaggcaggt ggagaggttc ggaggccagg cggagccggc cccgggcggc
     4321 ggggtgcgcg gctttttcgg attcccgacg gcgcacgagc acgacgcgcg acacgccgtg
     4381 cagtgcggcc tcgcgatcct ggccgaggcc tcgacggccc gcagccggaa ggaccggacc
     4441 cggctctccg tccgggcggg cgtgcatacg ggcatgagcc tggtccgaga ccggggaggc
     4501 cggatcgaca tccagggcgg cggctcggac gtggcggcgc ggctggccga atgcggcgct
     4561 tcgggcgaac tcgtcgtcgg caaggccacc caccgcctgg tccgtgacca cttcgagacc
     4621 gtgagtctcg gccttcgcga cgtccgcgga ttcaccgagg accagccgct cttcgcggtc
     4681 acggccgagc ggacgacggt ggcggcctcg ggagccgttg ccggcagcgc tccctcgccg
     4741 ctcgtcggtc gcgacctgga actggacctg gtacgccgac gatggctcgc cgcccgcgcg
     4801 ggcagcgggc aggcgatcct gctcagcggg gagccgggca tagggaaatc gcgcctggtc
     4861 gaggcctttc tcgaggatct cgggagcgcg tggcattcgc ggctccactg ctcgcccctg
     4921 gaccagaaca ccgcgttcca cccgctggcg tcggccctgc gacagctggt acagcccgag
     4981 gctcccggcg ggaccgggat cgatccgggc gaactggacg ccttgctcgg cgcctgcggg
     5041 gtgactgccc gcgaagacgt cgccctggtc gcggagctca tggacgtgga gctcccctcc
     5101 gagtggccac gctccgcagc ctcgccgtcc gccaggcggc tccggctcct gcaggtcctc
     5161 gtccaggcac tcgtgatgcg gtaccgggac gcgtcgggcg tcgtcatcgt ggaggacctg
     5221 cactgggccg atccgtcgac cgtcgagtgg ctcgacctgc tggtcgagga gctgccctcc
     5281 ctacccgtcc tcctgctcgc cctcacgcgg cccccgagcc tgccggcctg gctgtcccgg
     5341 acacacgcct cggagatcgc gctcggcaag ctcacgccag aggacctggc gtcgatcacg
     5401 cggcaccggt cgggagacaa gccgcttccg cccgagatcg tccgtcacgt ggccgcgcgg
     5461 accgacggcg tgcccctctt tgccgaggag ctcaccagca tgatcctgga gtcggggctc
     5521 cttcgggacg cgggcgaccg gtacgagctg accggacccg tgccggagca ccttattccg
     5581 gccaccctcc acggatcgct catggcgcgg ctggaccagc aggcctccgc ccgccaggtg
     5641 gcccaggttg gggccgtcat cggccgacgc tttccgtacg cgctcctgcg ggccgtcgaa
     5701 ggggagggcg acgacgaggg tctcacgcag gcgctgaacc gcctggtgga ggcggagctc
     5761 gtgacgcggc gcggcttcat ccccgaggcg acgttcgagt tcaggcacgc gctcatccgt
     5821 gatgccgcct acgagagcct cttgcgcaag cgacgccggg agctgcacga gcgcaccgcg
     5881 gtcgccctct ccgaaggccc gttcgacgcg acgcccgccg cggtcgcccg gcactacacc
     5941 gaggccggtc ttcccgccaa ggcccttccg ctccgcgtcg aggccggacg gacggcgatg
     6001 cggcggaacg ccaaccgcga ggcggtcgag cagctccggg ccgggctcga gctcctcgcc
     6061 gagctcgacg aggggcccgg gcgcgacgtg cacgaactgg agatcctgag cctgctcgga
     6121 cccgcggtca tgatgaccaa gggctattcg gaccccgagg gcgaggagat cttcctgcgc
     6181 atccaggaaa tctgccggcg cgtgaacgcc atgcagccgc tcttcttctc gctgtacgga
     6241 ctggtctcgg tatacctcat ccggggcgag tacgaccggg cgttcgacat cctggcacgg
     6301 agcctcgaga cggcccagga ggtcggagaa acccgctacc tggtcatgag ccacaacgcg
     6361 acgggcgtcg cccgcatgtt cgcgggccag ccggccgagg ccctgcccca tctcgatgcc
     6421 gtgctccgcc tgcacgacaa cgagcgcgat gcggacctgg cgctcctcgg ttggggcagg
     6481 ctcgacgtga gcgccgccac ctcgcgccag ctcgctcttc aactcctcgg cgattccgac
     6541 ggagccctcg cccagtgccg ggaggccctc gccgcggccg agggctcgcc gaaccacatg
     6601 accgtctacc acgcgcacct ctacgcaggg ctgctgtacc tgatgcggcg cgagtgggcg
     6661 gcctgccggg acgtcatgca ggcctacctg ccgatggcga tcgagtacgg ggacccgttt
     6721 tacatcgcga tcgcgactgt cgtgtcgagc ctcgcgcaca gggaccccgg ccaggcggac
     6781 acgttccgcc tggcgggaac ggtccttgac cagatgcggt cggccggcta tgccctgggg
     6841 ctgggtttcc tgctctgcca ccaggccgac gcgctcctgg cgaagggcga aaccgcggag
     6901 gcggcctccg tcgtggacga cgccctgcgg cacctcgggg agcgcaacgg ggaattctgg
     6961 gaggccgagg tcttccgcct caagggcgcc gtcgaggccg cgaacggccg cgacccggag
     7021 ccctggatcg gccgggcgct cgagacgtcc cggcggcagg gagcccggtg gctggagctg
     7081 cgggcgacgg ccgacctcgc gcggcgccgc ctcgaggcgg gccgggcgga cgaggcgcgt
     7141 acgctcatcc gcgatgcgct ggcgggcctg gccgggggcg acggactgcc tgacctggaa
     7201 gaggcccgcg ccctggccgg ccgcctcgag tccgcctgag cgctcaggcc gcggccccgg
     7261 ctcgccaggc gtcgaagacg ggctcggtcg cgtccccggg aagcccctcg acgaggcccg
     7321 caagcgcccg gtcgcacgcg tggaagccca gcgcgcggta ggcctccagg tcctcctcgc
     7381 cgaagaactg gtcggccgtc gactggtgcg ggaaggccgg gttggcgtgg cggtagtgcc
     7441 ggatgagctc gccctcgtcc ccggtgagcg acgatctcag gtacaggagg atgccgcgct
     7501 ctcccgaccc gtaatc
//
LOCUS       DBNHQZ010000453         3196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rhodothermales bacterium isolate MAG04990
            SRR25158347_k127_2844164, whole genome shotgun sequence.
ACCESSION   DBNHQZ010000453 DBNHQZ010000000
VERSION     DBNHQZ010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414398
            Sequence Read Archive: SRR25158347
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodothermales bacterium (soil metagenome)
  ORGANISM  Rhodothermales bacterium
            Bacteria; Pseudomonadati; Rhodothermota; Rhodothermia;
            Rhodothermales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:21:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,452
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Rhodothermales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158347_k127_2844164"
                     /isolate="MAG04990"
                     /isolation_source="soil"
                     /db_xref="taxon:2306054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.09 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="AC16YU_12385"
     CDS             <1..1212
                     /locus_tag="AC16YU_12385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR6066069.1"
                     /translation="AFERGTSVYLVDRVIPMLPERLSADLCSLRPDEERLAFSCIMTL
                     DAEGTVLDYRITESVIRSRARLTYEEAQEILDDPARAGEADADVVAALRALARLAEVV
                     RRRRMDHGAIDFDLPEVRVVLDEAGIVTEVVRKVRLGTHRLIEEFMLLANRTVASHAD
                     RRHPDAAFVYRVHDRPNTERMGQLVSFVRAFGYKVTSQGGKISSQELNRLLGEARGRP
                     EEPVIEEAALRAMAKAVYSPENVGHFGLAFTHYTHFTSPIRRYPDLMVHRLLKDLAAG
                     RPTPGFGDLKAACEHASERERAADDAQRESVKLKQVEYMQQHVGDRFTGVVSGVTRFG
                     VFVELDALLVEGLIHVRELSDDYYEYDETTFSLTGRYKGRTFRLGDSLDIIVAAANTE
                     TREIDFVLPGA"
     gene            1217..2725
                     /locus_tag="AC16YU_12390"
     CDS             1217..2725
                     /locus_tag="AC16YU_12390"
                     /inference="COORDINATES: protein motif:HMM:NF039827.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HIR6066070.1"
                     /translation="MRKPLGAHTVLALLVRNQFRRLRRTVGRPGDRLMMLIVGVLGFY
                     MAFTLVVLGVYFPKFALKFGVETGAVELVNAYAVSILFGLFGLRFLFQKTPRLQLLPY
                     LHLPVGRGYLIGFFVASTTLSLHNLFPLLFTVPLAVFHIAPEYGTAGAVAWTLGGFAL
                     LLVSNFANLYMRTLLQKQEGLFLTILGSFMVITFLDEYVGAHVIRTISEAVFTPLLRA
                     DALTIASLGVFVLGTAVFAAAAIYESVRGMTFDLVAPRRAGRLYELAERWSVVGHLVW
                     LELRLMSRNRRPRHYLVVSLLFSTVYLVFLLASPAVFGEFVFAAVMGLFASGGFVLNY
                     GQLMFGWDSMYFDGIIGRDLRMRVLARSKVLVLQGSCLFLFVISLPLFLWLRPDLVPL
                     HVAFLFYNAGVTSQLIMELAVRNSDPIDLGRNGGVLNYEGFSIRHWLWFFPTALPPTL
                     LLLALKEQEALAWTLLAVVGLVGLAATEMWIRRHAAALESRKHYMAAGFRGR"
     gene            2722..>3196
                     /locus_tag="AC16YU_12395"
     CDS             2722..>3196
                     /locus_tag="AC16YU_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6066071.1"
                     /translation="MMIRVNQLLKQYGGRTVLDVPSWHVAPGEIVGLVGSNGAGKTTF
                     LRLVLDLIQPDNGSVLIDGYDVSESDDWKSFTGSYLDERYLIGFLTAEEFLDFIRTVY
                     GLSRAQAETSLVAYRSFLPENGSARYLQELSTGNAKKVGITAALFFEPRLVILDEP"
BASE COUNT          441 a         1135 c         1045 g          575 t
ORIGIN      
        1 gcgttcgagc gcgggaccag cgtgtacctc gtggaccgcg tcatcccgat gctgcccgag
       61 cggctgtccg cggacctgtg ctcgctccgc cccgacgagg agcggctcgc gttctcgtgc
      121 atcatgacgc tcgacgcaga ggggacggtc ctggactacc ggatcacgga gtccgtcatc
      181 cgctcgcgcg cccggctcac ctacgaggag gcccaggaga tcctggacga tcccgcccgg
      241 gccggcgagg cggacgcgga cgtggtggcg gccctccggg cgctcgcgcg gctcgccgag
      301 gtcgtccgcc gccggcgcat ggaccacggg gcgatcgact tcgacctgcc cgaggtccgg
      361 gtggtgctcg acgaggcggg catcgtcacc gaggtcgtgc gcaaggtccg gctcgggacg
      421 caccggctca tcgaggagtt catgctgctc gcgaaccgga cggtggcctc gcacgcggac
      481 cggcgtcatc ccgacgccgc gttcgtgtac cgcgtgcacg accgcccgaa caccgagcgc
      541 atgggccagc tcgtgtcctt cgtccgcgcg ttcgggtaca aggtcacctc ccagggcggg
      601 aagatctcca gccaggagct gaaccggctg ctcggcgagg cccgtggacg ccccgaggag
      661 ccggtcatcg aggaggcggc cctccgcgcg atggccaagg ccgtgtacag ccccgagaac
      721 gtcggccact tcgggctcgc cttcacgcac tacacccatt tcacgagccc gatccgccgc
      781 tatcccgacc tcatggtgca ccggctgctc aaggacctgg ccgcggggcg tcccacgccc
      841 gggttcggcg acctgaaggc ggcctgcgag cacgcctccg agcgggagcg ggcggccgac
      901 gacgcccagc gcgagtccgt gaagctgaag caggtcgagt acatgcagca gcacgtgggc
      961 gaccgtttca cgggcgtcgt gagcggcgtc acgcggttcg gcgtgttcgt ggagctcgat
     1021 gcgctgctcg tggagggact cattcacgtt cgagaacttt cggacgatta ctacgagtac
     1081 gacgaaacca ccttttccct gaccggccga tacaaagggc gcaccttccg cctgggcgat
     1141 tccctcgaca tcatcgtggc ggcggccaac accgaaacgc gggaaatcga cttcgtgctg
     1201 cccggcgcct gagcgcatgc gcaaacccct cggcgcccat accgtcctcg ccctgctcgt
     1261 ccggaaccag ttccggcgcc tgcgccgcac cgtgggccgc ccgggcgacc gcctgatgat
     1321 gctcatcgtc ggcgtgctgg gcttctacat ggccttcacg ctcgtggtgc tcggggtcta
     1381 cttcccgaag ttcgcgctga agttcggcgt cgagacgggg gccgtcgagc tcgtgaacgc
     1441 ctacgccgtg agcatcctgt tcgggctctt cggcctgcgc tttctcttcc agaagacacc
     1501 ccgcctgcag ctcctgccct acctgcacct gcccgtgggg cgcggctacc tgatcgggtt
     1561 cttcgtggcc tcgaccacgc tgtcgctgca caacctcttc ccgctgctct tcacggtccc
     1621 gctcgccgtc ttccacatcg ccccggaata cggcacggcg ggggcggtcg cgtggaccct
     1681 cggcgggttc gcgctgctcc tggtctcgaa tttcgcgaac ctgtacatgc ggacgctgct
     1741 gcagaagcag gagggcctct tcctgacgat cctgggctcc ttcatggtca tcacgttcct
     1801 ggacgagtac gtcggggcgc acgtgatccg gaccatttcg gaggccgtgt tcacgccgct
     1861 cctccgggcg gacgccctca cgatcgcctc gctcggcgtg ttcgtcctcg ggacggcggt
     1921 tttcgccgcg gcggccatct acgaatcggt ccgcggcatg acgttcgacc tcgtggcccc
     1981 gcgacgggcc ggccgcctgt acgagctggc cgagcgctgg agcgtggtcg gccacctcgt
     2041 gtggctcgag ctccggctga tgtcgcgcaa ccggcggccc cggcactacc tggtggtctc
     2101 gctgctcttc agcacggtct acctcgtgtt cctcctcgcg agccccgcgg tgttcggcga
     2161 gttcgtgttc gcggccgtca tggggctctt cgcctcgggc ggcttcgtgc tgaactacgg
     2221 gcagctgatg ttcgggtggg actcgatgta cttcgacggc atcatcgggc gcgacctgcg
     2281 catgcgggtc ctggcccgca gcaaggtgct ggtgctccag ggctcgtgcc tgttcctgtt
     2341 cgtgatcagc ctcccgctgt tcctgtggct ccgtccggac ctcgtgccgc ttcacgtggc
     2401 cttcctgttc tacaacgcgg gcgtcacgag ccagctcatc atggagctcg cggtgcgcaa
     2461 cagcgacccg atcgacctcg gccgcaacgg cggcgtgctc aactacgagg gcttctcgat
     2521 ccggcactgg ctctggttct tcccgacggc cctgcccccg accctcctgc tcctcgcgct
     2581 caaggagcag gaggcgctcg cgtggaccct tctggccgtt gtcgggttgg tggggctcgc
     2641 cgccaccgag atgtggatcc gccgccacgc ggccgccctc gaatccagga aacactacat
     2701 ggccgccggc ttccgtggtc gatgatgatc cgcgtcaacc agctcctgaa gcagtacgga
     2761 ggccgcaccg tgctcgacgt cccgtcgtgg cacgtggcgc ccggtgagat cgtcgggctg
     2821 gtcggctcga acggagccgg caagaccact ttcctgcgcc tggtgctgga cctcatccag
     2881 ccggacaacg ggtccgtgct gatcgacgga tacgacgtct cggagtccga cgactggaag
     2941 agcttcacgg gctcctatct cgacgaacgc tacctcatcg gcttcctgac ggccgaggag
     3001 ttccttgact tcatccggac ggtctacggg ctgtcgcggg cccaggccga gacctcgctc
     3061 gtcgcctacc ggagcttcct ccccgagaac ggctccgcgc gctacctgca ggagctctcc
     3121 accggaaacg cgaagaaagt gggcatcacg gcggcgttgt tcttcgagcc ccggctggtg
     3181 atcctggacg agccct
//
